FASTKD2 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	FASTKD2
Entrez Gene ID	22868
Full Name	FAST kinase domains 2
Synonyms	KIAA0971
General protein information	Preferred Names FAST kinase domains 2 Names FAST kinase domain-containing protein 2, mitochondrial
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 2 Map Location: 2q33.3
Summary	This gene encodes a protein that is localized in the mitochondrial inner compartment and that may play a role in mitochondrial apoptosis. Nonsense mutations have been reported to result in cytochrome c oxidase deficiency. [provided by RefSeq, Oct 2008].
Disorder MIM:	612322
Disorder Html:	?Mitochondrial complex IV deficiency, 220110 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001136193.2	NP_001129665.1	FAST kinase domain-containing protein 2, mitochondrial
NM_001136194.1	NP_001129666.1	FAST kinase domain-containing protein 2, mitochondrial
NM_001136193.1	NP_001129665.1	FAST kinase domain-containing protein 2, mitochondrial
NM_014929.3	NP_055744.2	FAST kinase domain-containing protein 2, mitochondrial

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	FASTKD2	XP_003309502.1
	FASTKD2	XP_002799081.1
Bos taurus (cattle)	FASTKD2	NP_001095587.1
Danio rerio (zebrafish)	fastkd2	XP_692810.5
Xenopus tropicalis (tropical clawed frog)	fastkd2	XP_002937162.1
Mus musculus (house mouse)	Fastkd2	NP_766010.1
Gallus gallus (chicken)	FASTKD2	XP_421951.2
Homo sapiens (human)	FASTKD2	NP_001129665.1
Canis lupus familiaris (dog)	FASTKD2	XP_005640642.1
Rattus norvegicus (Norway rat)	Fastkd2	NP_001009673.1

Gene Ontology
Bibliography

Related articles in PubMed

The FASTK family of proteins: emerging regulators of mitochondrial RNA biology.
Jourdain AA, Popow J, de la Fuente MA, Martinou JC, Anderson P, Simarro M
Nucleic acids research45(19)10941-10947(2017 Nov)

A Genome-wide CRISPR Death Screen Identifies Genes Essential for Oxidative Phosphorylation.
Arroyo JD, Jourdain AA, Calvo SE, Ballarano CA, Doench JG, Root DE, Mootha VK
Cell metabolism24(6)875-885(2016 Dec)

FASTKD2 is an RNA-binding protein required for mitochondrial RNA processing and translation.
Popow J, Alleaume AM, Curk T, Schwarzl T, Sauer S, Hentze MW
RNA (New York, N.Y.)21(11)1873-84(2015 Nov)

Mitochondrial RNA Granules Are Centers for Posttranscriptional RNA Processing and Ribosome Biogenesis.
Antonicka H, Shoubridge EA
Cell reports(2015 Feb)

Fas Activated Serine-Threonine Kinase Domains 2 (FASTKD2) mediates apoptosis of breast and prostate cancer cells through its novel FAST2 domain.
Das S, Yeung KT, Mahajan MA, Samuels HH
BMC cancer14852(2014 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Despite the fact that proteins of the FASTK family FASTKD1-5 share the same domains, they exhibit various-sometimes opposing-functions in almost all steps of mitochondrial RNA metabolism.
Title: The FASTK family of proteins: emerging regulators of mitochondrial RNA biology.

CRISPR-mediated deletion of FASTKD2 leads to aberrant processing.
Title: FASTKD2 is an RNA-binding protein required for mitochondrial RNA processing and translation.

Authors discovered a novel association of a polymorphism in the pro-apoptotic gene FASTKD2 (fas-activated serine/threonine kinase domains 2) with better memory performance
Title: FASTKD2 is associated with memory and hippocampal structure in older adults.

Fas Activated Serine-Threonine Kinase Domains 2 (FASTKD2) mediates apoptosis of breast and prostate cancer cells through its novel FAST2 domain.
Title: Fas Activated Serine-Threonine Kinase Domains 2 (FASTKD2) mediates apoptosis of breast and prostate cancer cells through its novel FAST2 domain.

regulation of FASTKD2 by NRIF3 and the DIF-1 complex acts as a novel death switch that selectively modulates apoptosis in breast cancer
Title: A novel transcription complex that selectively modulates apoptosis of breast cancer cells through regulation of FASTKD2.

The following FASTKD2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the FASTKD2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu09087	NM_001136193.2 Latest version!	Homo sapiens FAST kinase domains 2 (FASTKD2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu09087	NM_001136194.1 Latest version!	Homo sapiens FAST kinase domains 2 (FASTKD2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu09087	NM_014929.3 Latest version!	Homo sapiens FAST kinase domains 2 (FASTKD2), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
View Old Accession Versions >>
OHu09087	NM_001136193.1	Homo sapiens FAST kinase domains 2 (FASTKD2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

FASTKD2 ( NM_001136193.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001129665.1 Homo sapiens FAST kinase domains 2 (FASTKD2), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu09087
Clone ID Related Accession (Same CDS sequence)	NM_001136193.2 , NM_001136194.1 , NM_001136193.1 , NM_014929.3
Accession Version	NM_001136193.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2133bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-30
Organism	Homo sapiens(human)
Product	FAST kinase domain-containing protein 2, mitochondrial
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA021045.1, BC001544.1 and AC008269.4. On Nov 23, 2018 this sequence version replaced NM_001136193.1. Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. All three variants encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.187060.1, SRR1803612.163016.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000402774.8/ ENSP00000385990.3 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGTTGACAA CTTTGAAGCC ATTTGGAAGT GTTTCAGTGG AGAGCAAAAT GAATAACAAA 
GCGGGCTCCT TTTTCTGGAA CCTTAGACAA TTCAGTACAT TAGTTTCAAC AAGCAGAACT 
ATGAGGCTAT GTTGTTTGGG ACTTTGCAAA CCAAAAATAG TTCATTCAAA CTGGAACATT 
TTAAATAACT TTCATAACAG AATGCAATCA ACTGATATCA TTAGATATCT CTTTCAGGAT 
GCATTCATTT TTAAATCAGA TGTTGGCTTT CAAACAAAGG GCATAAGCAC TCTAACAGCC 
CTTAGAATTG AAAGACTACT TTATGCTAAA AGACTGTTTT TTGACTCAAA GCAGTCTCTT 
GTCCCTGTTG ATAAATCTGA TGATGAATTG AAGAAAGTAA ACCTTAATCA TGAAGTCTCC 
AATGAAGATG TTCTTACCAA GGAAACAAAA CCAAACCGTA TCAGCAGTAG AAAACTGTCT 
GAGGAATGTA ATTCCCTGAG TGATGTGTTA GATGCATTTT CAAAAGCGCC CACATTTCCT 
AGTAGCAACT ATTTCACAGC AATGTGGACA ATTGCCAAAA GACTGTCTGA TGACCAGAAG 
CGCTTTGAAA AACGACTGAT GTTTAGCCAC CCTGCATTTA ATCAGCTCTG TGAACATATG 
ATGAGAGAAG CCAAGATCAT GCAGTATAAG TACCTACTGT TCAGTCTTCA CGCCATAGTG 
AAGCTTGGAA TCCCTCAGAA CACTATTTTG GTGCAGACTT TGCTGAGGGT GACCCAGGAA 
CGTATCAATG AGTGTGATGA GATATGCCTT TCAGTTTTGT CAACTGTTTT AGAGGCAATG 
GAACCATGCA AGAATGTTCA TGTTCTACGA ACGGGATTCA GAATACTAGT TGATCAGCAA 
GTTTGGAAAA TAGAAGATGT CTTCACATTA CAAGTTGTGA TGAAGTGTAT TGGAAAAGAT 
GCACCGATTG CTCTTAAGAG GAAACTGGAG ATGAAAGCCT TGAGGGAATT AGACAGATTT 
TCTGTTTTGA ATAGCCAACA CATGTTTGAA GTACTAGCTG CCATGAATCA CCGATCTCTT 
ATACTCCTGG ATGAATGCAG TAAGGTGGTC CTAGATAATA TCCATGGGTG TCCTTTAAGA 
ATAATGATCA ACATATTGCA GTCCTGCAAA GACCTCCAGT ACCATAATTT GGATCTCTTC 
AAGGGACTTG CAGATTATGT GGCTGCAACT TTCGACATCT GGAAGTTCAG AAAAGTTCTT 
TTTATCCTCA TTTTATTTGA AAACCTTGGC TTTCGACCTG TTGGTTTAAT GGACCTGTTT 
ATGAAGAGAA TAGTAGAGGA TCCTGAATCC CTAAACATGA AAAACATTCT ATCTATTCTT 
CATACTTACT CTTCTCTCAA TCATGTCTAC AAATGCCAGA ACAAAGAACA GTTCGTGGAA 
GTTATGGCTA GTGCTCTGAC TGGTTATCTT CACACTATTT CTTCTGAAAA CTTATTGGAT 
GCAGTATATT CATTTTGCTT GATGAATTAC TTTCCCCTGG CTCCTTTTAA TCAGCTTCTG 
CAAAAAGACA TCATCAGTGA GCTGCTGACA TCAGATGACA TGAAGAATGC TTACAAGCTG 
CATACTTTGG ATACTTGTCT AAAACTTGAT GATACTGTCT ATCTGAGGGA CATAGCCTTG 
TCACTCCCAC AGCTGCCGCG GGAGCTGCCA TCGTCACATA CAAATGCAAA GGTGGCAGAG 
GTGCTGAGCA GCCTTCTGGG AGGTGAAGGA CACTTCTCAA AGGATGTGCA CTTGCCACAC 
AATTATCATA TTGATTTTGA AATCAGAATG GACACTAACA GGAATCAAGT GCTACCACTT 
TCTGATGTGG ATACAACTTC TGCTACAGAT ATTCAAAGAG TAGCTGTGCT ATGTGTTTCC 
AGATCTGCTT ATTGTTTGGG TTCAAGCCAC CCCAGAGGAT TCCTTGCTAT GAAAATGCGG 
CATTTGAATG CAATGGGTTT TCATGTGATC TTGGTCAATA ACTGGGAGAT GGACAAACTA 
GAGATGGAAG ATGCAGTCAC ATTTTTGAAG ACTAAAATCT ATTCAGTAGA AGCTCTTCCT 
GTTGCTGCTG TAAATGTGCA AAGCACACAA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001129665.1
CDS	193..2325
Translation	MLTTLKPFGSVSVESKMNNKAGSFFWNLRQFSTLVSTSRTMRLCCLGLCKPKIVHSNWNILNNFHNRMQSTDIIRYLFQDAFIFKSDVGFQTKGISTLTALRIERLLYAKRLFFDSKQSLVPVDKSDDELKKVNLNHEVSNEDVLTKETKPNRISSRKLSEECNSLSDVLDAFSKAPTFPSSNYFTAMWTIAKRLSDDQKRFEKRLMFSHPAFNQLCEHMMREAKIMQYKYLLFSLHAIVKLGIPQNTILVQTLLRVTQERINECDEICLSVLSTVLEAMEPCKNVHVLRTGFRILVDQQVWKIEDVFTLQVVMKCIGKDAPIALKRKLEMKALRELDRFSVLNSQHMFEVLAAMNHRSLILLDECSKVVLDNIHGCPLRIMINILQSCKDLQYHNLDLFKGLADYVAATFDIWKFRKVLFILILFENLGFRPVGLMDLFMKRIVEDPESLNMKNILSILHTYSSLNHVYKCQNKEQFVEVMASALTGYLHTISSENLLDAVYSFCLMNYFPLAPFNQLLQKDIISELLTSDDMKNAYKLHTLDTCLKLDDTVYLRDIALSLPQLPRELPSSHTNAKVAEVLSSLLGGEGHFSKDVHLPHNYHIDFEIRMDTNRNQVLPLSDVDTTSATDIQRVAVLCVSRSAYCLGSSHPRGFLAMKMRHLNAMGFHVILVNNWEMDKLEMEDAVTFLKTKIYSVEALPVAAVNVQSTQ

Target ORF information:

RefSeq Version	NM_001136193.2
Organism	Homo sapiens(human)
Definition	Homo sapiens FAST kinase domains 2 (FASTKD2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001136193.2

ORF Insert Sequence:

ATGTTGACAA CTTTGAAGCC ATTTGGAAGT GTTTCAGTGG AGAGCAAAAT GAATAACAAA 
GCGGGCTCCT TTTTCTGGAA CCTTAGACAA TTCAGTACAT TAGTTTCAAC AAGCAGAACT 
ATGAGGCTAT GTTGTTTGGG ACTTTGCAAA CCAAAAATAG TTCATTCAAA CTGGAACATT 
TTAAATAACT TTCATAACAG AATGCAATCA ACTGATATCA TTAGATATCT CTTTCAGGAT 
GCATTCATTT TTAAATCAGA TGTTGGCTTT CAAACAAAGG GCATAAGCAC TCTAACAGCC 
CTTAGAATTG AAAGACTACT TTATGCTAAA AGACTGTTTT TTGACTCAAA GCAGTCTCTT 
GTCCCTGTTG ATAAATCTGA TGATGAATTG AAGAAAGTAA ACCTTAATCA TGAAGTCTCC 
AATGAAGATG TTCTTACCAA GGAAACAAAA CCAAACCGTA TCAGCAGTAG AAAACTGTCT 
GAGGAATGTA ATTCCCTGAG TGATGTGTTA GATGCATTTT CAAAAGCGCC CACATTTCCT 
AGTAGCAACT ATTTCACAGC AATGTGGACA ATTGCCAAAA GACTGTCTGA TGACCAGAAG 
CGCTTTGAAA AACGACTGAT GTTTAGCCAC CCTGCATTTA ATCAGCTCTG TGAACATATG 
ATGAGAGAAG CCAAGATCAT GCAGTATAAG TACCTACTGT TCAGTCTTCA CGCCATAGTG 
AAGCTTGGAA TCCCTCAGAA CACTATTTTG GTGCAGACTT TGCTGAGGGT GACCCAGGAA 
CGTATCAATG AGTGTGATGA GATATGCCTT TCAGTTTTGT CAACTGTTTT AGAGGCAATG 
GAACCATGCA AGAATGTTCA TGTTCTACGA ACGGGATTCA GAATACTAGT TGATCAGCAA 
GTTTGGAAAA TAGAAGATGT CTTCACATTA CAAGTTGTGA TGAAGTGTAT TGGAAAAGAT 
GCACCGATTG CTCTTAAGAG GAAACTGGAG ATGAAAGCCT TGAGGGAATT AGACAGATTT 
TCTGTTTTGA ATAGCCAACA CATGTTTGAA GTACTAGCTG CCATGAATCA CCGATCTCTT 
ATACTCCTGG ATGAATGCAG TAAGGTGGTC CTAGATAATA TCCATGGGTG TCCTTTAAGA 
ATAATGATCA ACATATTGCA GTCCTGCAAA GACCTCCAGT ACCATAATTT GGATCTCTTC 
AAGGGACTTG CAGATTATGT GGCTGCAACT TTCGACATCT GGAAGTTCAG AAAAGTTCTT 
TTTATCCTCA TTTTATTTGA AAACCTTGGC TTTCGACCTG TTGGTTTAAT GGACCTGTTT 
ATGAAGAGAA TAGTAGAGGA TCCTGAATCC CTAAACATGA AAAACATTCT ATCTATTCTT 
CATACTTACT CTTCTCTCAA TCATGTCTAC AAATGCCAGA ACAAAGAACA GTTCGTGGAA 
GTTATGGCTA GTGCTCTGAC TGGTTATCTT CACACTATTT CTTCTGAAAA CTTATTGGAT 
GCAGTATATT CATTTTGCTT GATGAATTAC TTTCCCCTGG CTCCTTTTAA TCAGCTTCTG 
CAAAAAGACA TCATCAGTGA GCTGCTGACA TCAGATGACA TGAAGAATGC TTACAAGCTG 
CATACTTTGG ATACTTGTCT AAAACTTGAT GATACTGTCT ATCTGAGGGA CATAGCCTTG 
TCACTCCCAC AGCTGCCGCG GGAGCTGCCA TCGTCACATA CAAATGCAAA GGTGGCAGAG 
GTGCTGAGCA GCCTTCTGGG AGGTGAAGGA CACTTCTCAA AGGATGTGCA CTTGCCACAC 
AATTATCATA TTGATTTTGA AATCAGAATG GACACTAACA GGAATCAAGT GCTACCACTT 
TCTGATGTGG ATACAACTTC TGCTACAGAT ATTCAAAGAG TAGCTGTGCT ATGTGTTTCC 
AGATCTGCTT ATTGTTTGGG TTCAAGCCAC CCCAGAGGAT TCCTTGCTAT GAAAATGCGG 
CATTTGAATG CAATGGGTTT TCATGTGATC TTGGTCAATA ACTGGGAGAT GGACAAACTA 
GAGATGGAAG ATGCAGTCAC ATTTTTGAAG ACTAAAATCT ATTCAGTAGA AGCTCTTCCT 
GTTGCTGCTG TAAATGTGCA AAGCACACAA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FASTKD2 ( NM_001136194.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001129666.1 Homo sapiens FAST kinase domains 2 (FASTKD2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu09087
Clone ID Related Accession (Same CDS sequence)	NM_001136193.2 , NM_001136194.1 , NM_001136193.1 , NM_014929.3
Accession Version	NM_001136194.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2133bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-11
Organism	Homo sapiens(human)
Product	FAST kinase domain-containing protein 2, mitochondrial
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA475994.1, DA756417.1, BC001544.1 and AC008269.4. This sequence is a reference standard in the RefSeqGene project. Transcript Variant: This variant (1) represents the longest transcript. All three variants encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.238724.1, SRR1803614.31442.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTTGACAA CTTTGAAGCC ATTTGGAAGT GTTTCAGTGG AGAGCAAAAT GAATAACAAA 
GCGGGCTCCT TTTTCTGGAA CCTTAGACAA TTCAGTACAT TAGTTTCAAC AAGCAGAACT 
ATGAGGCTAT GTTGTTTGGG ACTTTGCAAA CCAAAAATAG TTCATTCAAA CTGGAACATT 
TTAAATAACT TTCATAACAG AATGCAATCA ACTGATATCA TTAGATATCT CTTTCAGGAT 
GCATTCATTT TTAAATCAGA TGTTGGCTTT CAAACAAAGG GCATAAGCAC TCTAACAGCC 
CTTAGAATTG AAAGACTACT TTATGCTAAA AGACTGTTTT TTGACTCAAA GCAGTCTCTT 
GTCCCTGTTG ATAAATCTGA TGATGAATTG AAGAAAGTAA ACCTTAATCA TGAAGTCTCC 
AATGAAGATG TTCTTACCAA GGAAACAAAA CCAAACCGTA TCAGCAGTAG AAAACTGTCT 
GAGGAATGTA ATTCCCTGAG TGATGTGTTA GATGCATTTT CAAAAGCGCC CACATTTCCT 
AGTAGCAACT ATTTCACAGC AATGTGGACA ATTGCCAAAA GACTGTCTGA TGACCAGAAG 
CGCTTTGAAA AACGACTGAT GTTTAGCCAC CCTGCATTTA ATCAGCTCTG TGAACATATG 
ATGAGAGAAG CCAAGATCAT GCAGTATAAG TACCTACTGT TCAGTCTTCA CGCCATAGTG 
AAGCTTGGAA TCCCTCAGAA CACTATTTTG GTGCAGACTT TGCTGAGGGT GACCCAGGAA 
CGTATCAATG AGTGTGATGA GATATGCCTT TCAGTTTTGT CAACTGTTTT AGAGGCAATG 
GAACCATGCA AGAATGTTCA TGTTCTACGA ACGGGATTCA GAATACTAGT TGATCAGCAA 
GTTTGGAAAA TAGAAGATGT CTTCACATTA CAAGTTGTGA TGAAGTGTAT TGGAAAAGAT 
GCACCGATTG CTCTTAAGAG GAAACTGGAG ATGAAAGCCT TGAGGGAATT AGACAGATTT 
TCTGTTTTGA ATAGCCAACA CATGTTTGAA GTACTAGCTG CCATGAATCA CCGATCTCTT 
ATACTCCTGG ATGAATGCAG TAAGGTGGTC CTAGATAATA TCCATGGGTG TCCTTTAAGA 
ATAATGATCA ACATATTGCA GTCCTGCAAA GACCTCCAGT ACCATAATTT GGATCTCTTC 
AAGGGACTTG CAGATTATGT GGCTGCAACT TTCGACATCT GGAAGTTCAG AAAAGTTCTT 
TTTATCCTCA TTTTATTTGA AAACCTTGGC TTTCGACCTG TTGGTTTAAT GGACCTGTTT 
ATGAAGAGAA TAGTAGAGGA TCCTGAATCC CTAAACATGA AAAACATTCT ATCTATTCTT 
CATACTTACT CTTCTCTCAA TCATGTCTAC AAATGCCAGA ACAAAGAACA GTTCGTGGAA 
GTTATGGCTA GTGCTCTGAC TGGTTATCTT CACACTATTT CTTCTGAAAA CTTATTGGAT 
GCAGTATATT CATTTTGCTT GATGAATTAC TTTCCCCTGG CTCCTTTTAA TCAGCTTCTG 
CAAAAAGACA TCATCAGTGA GCTGCTGACA TCAGATGACA TGAAGAATGC TTACAAGCTG 
CATACTTTGG ATACTTGTCT AAAACTTGAT GATACTGTCT ATCTGAGGGA CATAGCCTTG 
TCACTCCCAC AGCTGCCGCG GGAGCTGCCA TCGTCACATA CAAATGCAAA GGTGGCAGAG 
GTGCTGAGCA GCCTTCTGGG AGGTGAAGGA CACTTCTCAA AGGATGTGCA CTTGCCACAC 
AATTATCATA TTGATTTTGA AATCAGAATG GACACTAACA GGAATCAAGT GCTACCACTT 
TCTGATGTGG ATACAACTTC TGCTACAGAT ATTCAAAGAG TAGCTGTGCT ATGTGTTTCC 
AGATCTGCTT ATTGTTTGGG TTCAAGCCAC CCCAGAGGAT TCCTTGCTAT GAAAATGCGG 
CATTTGAATG CAATGGGTTT TCATGTGATC TTGGTCAATA ACTGGGAGAT GGACAAACTA 
GAGATGGAAG ATGCAGTCAC ATTTTTGAAG ACTAAAATCT ATTCAGTAGA AGCTCTTCCT 
GTTGCTGCTG TAAATGTGCA AAGCACACAA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001129666.1
CDS	426..2558
Translation	MLTTLKPFGSVSVESKMNNKAGSFFWNLRQFSTLVSTSRTMRLCCLGLCKPKIVHSNWNILNNFHNRMQSTDIIRYLFQDAFIFKSDVGFQTKGISTLTALRIERLLYAKRLFFDSKQSLVPVDKSDDELKKVNLNHEVSNEDVLTKETKPNRISSRKLSEECNSLSDVLDAFSKAPTFPSSNYFTAMWTIAKRLSDDQKRFEKRLMFSHPAFNQLCEHMMREAKIMQYKYLLFSLHAIVKLGIPQNTILVQTLLRVTQERINECDEICLSVLSTVLEAMEPCKNVHVLRTGFRILVDQQVWKIEDVFTLQVVMKCIGKDAPIALKRKLEMKALRELDRFSVLNSQHMFEVLAAMNHRSLILLDECSKVVLDNIHGCPLRIMINILQSCKDLQYHNLDLFKGLADYVAATFDIWKFRKVLFILILFENLGFRPVGLMDLFMKRIVEDPESLNMKNILSILHTYSSLNHVYKCQNKEQFVEVMASALTGYLHTISSENLLDAVYSFCLMNYFPLAPFNQLLQKDIISELLTSDDMKNAYKLHTLDTCLKLDDTVYLRDIALSLPQLPRELPSSHTNAKVAEVLSSLLGGEGHFSKDVHLPHNYHIDFEIRMDTNRNQVLPLSDVDTTSATDIQRVAVLCVSRSAYCLGSSHPRGFLAMKMRHLNAMGFHVILVNNWEMDKLEMEDAVTFLKTKIYSVEALPVAAVNVQSTQ

Target ORF information:

RefSeq Version	NM_001136194.1
Organism	Homo sapiens(human)
Definition	Homo sapiens FAST kinase domains 2 (FASTKD2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001136194.1

ORF Insert Sequence:

ATGTTGACAA CTTTGAAGCC ATTTGGAAGT GTTTCAGTGG AGAGCAAAAT GAATAACAAA 
GCGGGCTCCT TTTTCTGGAA CCTTAGACAA TTCAGTACAT TAGTTTCAAC AAGCAGAACT 
ATGAGGCTAT GTTGTTTGGG ACTTTGCAAA CCAAAAATAG TTCATTCAAA CTGGAACATT 
TTAAATAACT TTCATAACAG AATGCAATCA ACTGATATCA TTAGATATCT CTTTCAGGAT 
GCATTCATTT TTAAATCAGA TGTTGGCTTT CAAACAAAGG GCATAAGCAC TCTAACAGCC 
CTTAGAATTG AAAGACTACT TTATGCTAAA AGACTGTTTT TTGACTCAAA GCAGTCTCTT 
GTCCCTGTTG ATAAATCTGA TGATGAATTG AAGAAAGTAA ACCTTAATCA TGAAGTCTCC 
AATGAAGATG TTCTTACCAA GGAAACAAAA CCAAACCGTA TCAGCAGTAG AAAACTGTCT 
GAGGAATGTA ATTCCCTGAG TGATGTGTTA GATGCATTTT CAAAAGCGCC CACATTTCCT 
AGTAGCAACT ATTTCACAGC AATGTGGACA ATTGCCAAAA GACTGTCTGA TGACCAGAAG 
CGCTTTGAAA AACGACTGAT GTTTAGCCAC CCTGCATTTA ATCAGCTCTG TGAACATATG 
ATGAGAGAAG CCAAGATCAT GCAGTATAAG TACCTACTGT TCAGTCTTCA CGCCATAGTG 
AAGCTTGGAA TCCCTCAGAA CACTATTTTG GTGCAGACTT TGCTGAGGGT GACCCAGGAA 
CGTATCAATG AGTGTGATGA GATATGCCTT TCAGTTTTGT CAACTGTTTT AGAGGCAATG 
GAACCATGCA AGAATGTTCA TGTTCTACGA ACGGGATTCA GAATACTAGT TGATCAGCAA 
GTTTGGAAAA TAGAAGATGT CTTCACATTA CAAGTTGTGA TGAAGTGTAT TGGAAAAGAT 
GCACCGATTG CTCTTAAGAG GAAACTGGAG ATGAAAGCCT TGAGGGAATT AGACAGATTT 
TCTGTTTTGA ATAGCCAACA CATGTTTGAA GTACTAGCTG CCATGAATCA CCGATCTCTT 
ATACTCCTGG ATGAATGCAG TAAGGTGGTC CTAGATAATA TCCATGGGTG TCCTTTAAGA 
ATAATGATCA ACATATTGCA GTCCTGCAAA GACCTCCAGT ACCATAATTT GGATCTCTTC 
AAGGGACTTG CAGATTATGT GGCTGCAACT TTCGACATCT GGAAGTTCAG AAAAGTTCTT 
TTTATCCTCA TTTTATTTGA AAACCTTGGC TTTCGACCTG TTGGTTTAAT GGACCTGTTT 
ATGAAGAGAA TAGTAGAGGA TCCTGAATCC CTAAACATGA AAAACATTCT ATCTATTCTT 
CATACTTACT CTTCTCTCAA TCATGTCTAC AAATGCCAGA ACAAAGAACA GTTCGTGGAA 
GTTATGGCTA GTGCTCTGAC TGGTTATCTT CACACTATTT CTTCTGAAAA CTTATTGGAT 
GCAGTATATT CATTTTGCTT GATGAATTAC TTTCCCCTGG CTCCTTTTAA TCAGCTTCTG 
CAAAAAGACA TCATCAGTGA GCTGCTGACA TCAGATGACA TGAAGAATGC TTACAAGCTG 
CATACTTTGG ATACTTGTCT AAAACTTGAT GATACTGTCT ATCTGAGGGA CATAGCCTTG 
TCACTCCCAC AGCTGCCGCG GGAGCTGCCA TCGTCACATA CAAATGCAAA GGTGGCAGAG 
GTGCTGAGCA GCCTTCTGGG AGGTGAAGGA CACTTCTCAA AGGATGTGCA CTTGCCACAC 
AATTATCATA TTGATTTTGA AATCAGAATG GACACTAACA GGAATCAAGT GCTACCACTT 
TCTGATGTGG ATACAACTTC TGCTACAGAT ATTCAAAGAG TAGCTGTGCT ATGTGTTTCC 
AGATCTGCTT ATTGTTTGGG TTCAAGCCAC CCCAGAGGAT TCCTTGCTAT GAAAATGCGG 
CATTTGAATG CAATGGGTTT TCATGTGATC TTGGTCAATA ACTGGGAGAT GGACAAACTA 
GAGATGGAAG ATGCAGTCAC ATTTTTGAAG ACTAAAATCT ATTCAGTAGA AGCTCTTCCT 
GTTGCTGCTG TAAATGTGCA AAGCACACAA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FASTKD2 ( NM_001136193.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001129665.1 Homo sapiens FAST kinase domains 2 (FASTKD2), transcript variant 2, mRNA.

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CloneID	OHu09087
Clone ID Related Accession (Same CDS sequence)	NM_001136193.2 , NM_001136194.1 , NM_001136193.1 , NM_014929.3
Accession Version	NM_001136193.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2133bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-05
Organism	Homo sapiens(human)
Product	FAST kinase domain-containing protein 2, mitochondrial
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA475994.1, DA021045.1, BC001544.1 and AC008269.4. Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. All three variants encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.163016.1, SRR1660805.22485.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTTGACAA CTTTGAAGCC ATTTGGAAGT GTTTCAGTGG AGAGCAAAAT GAATAACAAA 
GCGGGCTCCT TTTTCTGGAA CCTTAGACAA TTCAGTACAT TAGTTTCAAC AAGCAGAACT 
ATGAGGCTAT GTTGTTTGGG ACTTTGCAAA CCAAAAATAG TTCATTCAAA CTGGAACATT 
TTAAATAACT TTCATAACAG AATGCAATCA ACTGATATCA TTAGATATCT CTTTCAGGAT 
GCATTCATTT TTAAATCAGA TGTTGGCTTT CAAACAAAGG GCATAAGCAC TCTAACAGCC 
CTTAGAATTG AAAGACTACT TTATGCTAAA AGACTGTTTT TTGACTCAAA GCAGTCTCTT 
GTCCCTGTTG ATAAATCTGA TGATGAATTG AAGAAAGTAA ACCTTAATCA TGAAGTCTCC 
AATGAAGATG TTCTTACCAA GGAAACAAAA CCAAACCGTA TCAGCAGTAG AAAACTGTCT 
GAGGAATGTA ATTCCCTGAG TGATGTGTTA GATGCATTTT CAAAAGCGCC CACATTTCCT 
AGTAGCAACT ATTTCACAGC AATGTGGACA ATTGCCAAAA GACTGTCTGA TGACCAGAAG 
CGCTTTGAAA AACGACTGAT GTTTAGCCAC CCTGCATTTA ATCAGCTCTG TGAACATATG 
ATGAGAGAAG CCAAGATCAT GCAGTATAAG TACCTACTGT TCAGTCTTCA CGCCATAGTG 
AAGCTTGGAA TCCCTCAGAA CACTATTTTG GTGCAGACTT TGCTGAGGGT GACCCAGGAA 
CGTATCAATG AGTGTGATGA GATATGCCTT TCAGTTTTGT CAACTGTTTT AGAGGCAATG 
GAACCATGCA AGAATGTTCA TGTTCTACGA ACGGGATTCA GAATACTAGT TGATCAGCAA 
GTTTGGAAAA TAGAAGATGT CTTCACATTA CAAGTTGTGA TGAAGTGTAT TGGAAAAGAT 
GCACCGATTG CTCTTAAGAG GAAACTGGAG ATGAAAGCCT TGAGGGAATT AGACAGATTT 
TCTGTTTTGA ATAGCCAACA CATGTTTGAA GTACTAGCTG CCATGAATCA CCGATCTCTT 
ATACTCCTGG ATGAATGCAG TAAGGTGGTC CTAGATAATA TCCATGGGTG TCCTTTAAGA 
ATAATGATCA ACATATTGCA GTCCTGCAAA GACCTCCAGT ACCATAATTT GGATCTCTTC 
AAGGGACTTG CAGATTATGT GGCTGCAACT TTCGACATCT GGAAGTTCAG AAAAGTTCTT 
TTTATCCTCA TTTTATTTGA AAACCTTGGC TTTCGACCTG TTGGTTTAAT GGACCTGTTT 
ATGAAGAGAA TAGTAGAGGA TCCTGAATCC CTAAACATGA AAAACATTCT ATCTATTCTT 
CATACTTACT CTTCTCTCAA TCATGTCTAC AAATGCCAGA ACAAAGAACA GTTCGTGGAA 
GTTATGGCTA GTGCTCTGAC TGGTTATCTT CACACTATTT CTTCTGAAAA CTTATTGGAT 
GCAGTATATT CATTTTGCTT GATGAATTAC TTTCCCCTGG CTCCTTTTAA TCAGCTTCTG 
CAAAAAGACA TCATCAGTGA GCTGCTGACA TCAGATGACA TGAAGAATGC TTACAAGCTG 
CATACTTTGG ATACTTGTCT AAAACTTGAT GATACTGTCT ATCTGAGGGA CATAGCCTTG 
TCACTCCCAC AGCTGCCGCG GGAGCTGCCA TCGTCACATA CAAATGCAAA GGTGGCAGAG 
GTGCTGAGCA GCCTTCTGGG AGGTGAAGGA CACTTCTCAA AGGATGTGCA CTTGCCACAC 
AATTATCATA TTGATTTTGA AATCAGAATG GACACTAACA GGAATCAAGT GCTACCACTT 
TCTGATGTGG ATACAACTTC TGCTACAGAT ATTCAAAGAG TAGCTGTGCT ATGTGTTTCC 
AGATCTGCTT ATTGTTTGGG TTCAAGCCAC CCCAGAGGAT TCCTTGCTAT GAAAATGCGG 
CATTTGAATG CAATGGGTTT TCATGTGATC TTGGTCAATA ACTGGGAGAT GGACAAACTA 
GAGATGGAAG ATGCAGTCAC ATTTTTGAAG ACTAAAATCT ATTCAGTAGA AGCTCTTCCT 
GTTGCTGCTG TAAATGTGCA AAGCACACAA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001129665.1
CDS	411..2543
Translation	MLTTLKPFGSVSVESKMNNKAGSFFWNLRQFSTLVSTSRTMRLCCLGLCKPKIVHSNWNILNNFHNRMQSTDIIRYLFQDAFIFKSDVGFQTKGISTLTALRIERLLYAKRLFFDSKQSLVPVDKSDDELKKVNLNHEVSNEDVLTKETKPNRISSRKLSEECNSLSDVLDAFSKAPTFPSSNYFTAMWTIAKRLSDDQKRFEKRLMFSHPAFNQLCEHMMREAKIMQYKYLLFSLHAIVKLGIPQNTILVQTLLRVTQERINECDEICLSVLSTVLEAMEPCKNVHVLRTGFRILVDQQVWKIEDVFTLQVVMKCIGKDAPIALKRKLEMKALRELDRFSVLNSQHMFEVLAAMNHRSLILLDECSKVVLDNIHGCPLRIMINILQSCKDLQYHNLDLFKGLADYVAATFDIWKFRKVLFILILFENLGFRPVGLMDLFMKRIVEDPESLNMKNILSILHTYSSLNHVYKCQNKEQFVEVMASALTGYLHTISSENLLDAVYSFCLMNYFPLAPFNQLLQKDIISELLTSDDMKNAYKLHTLDTCLKLDDTVYLRDIALSLPQLPRELPSSHTNAKVAEVLSSLLGGEGHFSKDVHLPHNYHIDFEIRMDTNRNQVLPLSDVDTTSATDIQRVAVLCVSRSAYCLGSSHPRGFLAMKMRHLNAMGFHVILVNNWEMDKLEMEDAVTFLKTKIYSVEALPVAAVNVQSTQ

Target ORF information:

RefSeq Version	NM_001136193.1
Organism	Homo sapiens(human)
Definition	Homo sapiens FAST kinase domains 2 (FASTKD2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001136193.1

ORF Insert Sequence:

ATGTTGACAA CTTTGAAGCC ATTTGGAAGT GTTTCAGTGG AGAGCAAAAT GAATAACAAA 
GCGGGCTCCT TTTTCTGGAA CCTTAGACAA TTCAGTACAT TAGTTTCAAC AAGCAGAACT 
ATGAGGCTAT GTTGTTTGGG ACTTTGCAAA CCAAAAATAG TTCATTCAAA CTGGAACATT 
TTAAATAACT TTCATAACAG AATGCAATCA ACTGATATCA TTAGATATCT CTTTCAGGAT 
GCATTCATTT TTAAATCAGA TGTTGGCTTT CAAACAAAGG GCATAAGCAC TCTAACAGCC 
CTTAGAATTG AAAGACTACT TTATGCTAAA AGACTGTTTT TTGACTCAAA GCAGTCTCTT 
GTCCCTGTTG ATAAATCTGA TGATGAATTG AAGAAAGTAA ACCTTAATCA TGAAGTCTCC 
AATGAAGATG TTCTTACCAA GGAAACAAAA CCAAACCGTA TCAGCAGTAG AAAACTGTCT 
GAGGAATGTA ATTCCCTGAG TGATGTGTTA GATGCATTTT CAAAAGCGCC CACATTTCCT 
AGTAGCAACT ATTTCACAGC AATGTGGACA ATTGCCAAAA GACTGTCTGA TGACCAGAAG 
CGCTTTGAAA AACGACTGAT GTTTAGCCAC CCTGCATTTA ATCAGCTCTG TGAACATATG 
ATGAGAGAAG CCAAGATCAT GCAGTATAAG TACCTACTGT TCAGTCTTCA CGCCATAGTG 
AAGCTTGGAA TCCCTCAGAA CACTATTTTG GTGCAGACTT TGCTGAGGGT GACCCAGGAA 
CGTATCAATG AGTGTGATGA GATATGCCTT TCAGTTTTGT CAACTGTTTT AGAGGCAATG 
GAACCATGCA AGAATGTTCA TGTTCTACGA ACGGGATTCA GAATACTAGT TGATCAGCAA 
GTTTGGAAAA TAGAAGATGT CTTCACATTA CAAGTTGTGA TGAAGTGTAT TGGAAAAGAT 
GCACCGATTG CTCTTAAGAG GAAACTGGAG ATGAAAGCCT TGAGGGAATT AGACAGATTT 
TCTGTTTTGA ATAGCCAACA CATGTTTGAA GTACTAGCTG CCATGAATCA CCGATCTCTT 
ATACTCCTGG ATGAATGCAG TAAGGTGGTC CTAGATAATA TCCATGGGTG TCCTTTAAGA 
ATAATGATCA ACATATTGCA GTCCTGCAAA GACCTCCAGT ACCATAATTT GGATCTCTTC 
AAGGGACTTG CAGATTATGT GGCTGCAACT TTCGACATCT GGAAGTTCAG AAAAGTTCTT 
TTTATCCTCA TTTTATTTGA AAACCTTGGC TTTCGACCTG TTGGTTTAAT GGACCTGTTT 
ATGAAGAGAA TAGTAGAGGA TCCTGAATCC CTAAACATGA AAAACATTCT ATCTATTCTT 
CATACTTACT CTTCTCTCAA TCATGTCTAC AAATGCCAGA ACAAAGAACA GTTCGTGGAA 
GTTATGGCTA GTGCTCTGAC TGGTTATCTT CACACTATTT CTTCTGAAAA CTTATTGGAT 
GCAGTATATT CATTTTGCTT GATGAATTAC TTTCCCCTGG CTCCTTTTAA TCAGCTTCTG 
CAAAAAGACA TCATCAGTGA GCTGCTGACA TCAGATGACA TGAAGAATGC TTACAAGCTG 
CATACTTTGG ATACTTGTCT AAAACTTGAT GATACTGTCT ATCTGAGGGA CATAGCCTTG 
TCACTCCCAC AGCTGCCGCG GGAGCTGCCA TCGTCACATA CAAATGCAAA GGTGGCAGAG 
GTGCTGAGCA GCCTTCTGGG AGGTGAAGGA CACTTCTCAA AGGATGTGCA CTTGCCACAC 
AATTATCATA TTGATTTTGA AATCAGAATG GACACTAACA GGAATCAAGT GCTACCACTT 
TCTGATGTGG ATACAACTTC TGCTACAGAT ATTCAAAGAG TAGCTGTGCT ATGTGTTTCC 
AGATCTGCTT ATTGTTTGGG TTCAAGCCAC CCCAGAGGAT TCCTTGCTAT GAAAATGCGG 
CATTTGAATG CAATGGGTTT TCATGTGATC TTGGTCAATA ACTGGGAGAT GGACAAACTA 
GAGATGGAAG ATGCAGTCAC ATTTTTGAAG ACTAAAATCT ATTCAGTAGA AGCTCTTCCT 
GTTGCTGCTG TAAATGTGCA AAGCACACAA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FASTKD2 ( NM_014929.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_055744.2 Homo sapiens FAST kinase domains 2 (FASTKD2), transcript variant 3, mRNA.

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CloneID	OHu09087
Clone ID Related Accession (Same CDS sequence)	NM_001136193.2 , NM_001136194.1 , NM_001136193.1 , NM_014929.3
Accession Version	NM_014929.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2133bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-12
Organism	Homo sapiens(human)
Product	FAST kinase domain-containing protein 2, mitochondrial
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA475994.1, BC001544.1 and AC008269.4. On Oct 25, 2008 this sequence version replaced NM_014929.2. Transcript Variant: This variant (3) differs in the 5' UTR compared to variant 1. All three variants encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.228720.1, SRR1803616.259398.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTTGACAA CTTTGAAGCC ATTTGGAAGT GTTTCAGTGG AGAGCAAAAT GAATAACAAA 
GCGGGCTCCT TTTTCTGGAA CCTTAGACAA TTCAGTACAT TAGTTTCAAC AAGCAGAACT 
ATGAGGCTAT GTTGTTTGGG ACTTTGCAAA CCAAAAATAG TTCATTCAAA CTGGAACATT 
TTAAATAACT TTCATAACAG AATGCAATCA ACTGATATCA TTAGATATCT CTTTCAGGAT 
GCATTCATTT TTAAATCAGA TGTTGGCTTT CAAACAAAGG GCATAAGCAC TCTAACAGCC 
CTTAGAATTG AAAGACTACT TTATGCTAAA AGACTGTTTT TTGACTCAAA GCAGTCTCTT 
GTCCCTGTTG ATAAATCTGA TGATGAATTG AAGAAAGTAA ACCTTAATCA TGAAGTCTCC 
AATGAAGATG TTCTTACCAA GGAAACAAAA CCAAACCGTA TCAGCAGTAG AAAACTGTCT 
GAGGAATGTA ATTCCCTGAG TGATGTGTTA GATGCATTTT CAAAAGCGCC CACATTTCCT 
AGTAGCAACT ATTTCACAGC AATGTGGACA ATTGCCAAAA GACTGTCTGA TGACCAGAAG 
CGCTTTGAAA AACGACTGAT GTTTAGCCAC CCTGCATTTA ATCAGCTCTG TGAACATATG 
ATGAGAGAAG CCAAGATCAT GCAGTATAAG TACCTACTGT TCAGTCTTCA CGCCATAGTG 
AAGCTTGGAA TCCCTCAGAA CACTATTTTG GTGCAGACTT TGCTGAGGGT GACCCAGGAA 
CGTATCAATG AGTGTGATGA GATATGCCTT TCAGTTTTGT CAACTGTTTT AGAGGCAATG 
GAACCATGCA AGAATGTTCA TGTTCTACGA ACGGGATTCA GAATACTAGT TGATCAGCAA 
GTTTGGAAAA TAGAAGATGT CTTCACATTA CAAGTTGTGA TGAAGTGTAT TGGAAAAGAT 
GCACCGATTG CTCTTAAGAG GAAACTGGAG ATGAAAGCCT TGAGGGAATT AGACAGATTT 
TCTGTTTTGA ATAGCCAACA CATGTTTGAA GTACTAGCTG CCATGAATCA CCGATCTCTT 
ATACTCCTGG ATGAATGCAG TAAGGTGGTC CTAGATAATA TCCATGGGTG TCCTTTAAGA 
ATAATGATCA ACATATTGCA GTCCTGCAAA GACCTCCAGT ACCATAATTT GGATCTCTTC 
AAGGGACTTG CAGATTATGT GGCTGCAACT TTCGACATCT GGAAGTTCAG AAAAGTTCTT 
TTTATCCTCA TTTTATTTGA AAACCTTGGC TTTCGACCTG TTGGTTTAAT GGACCTGTTT 
ATGAAGAGAA TAGTAGAGGA TCCTGAATCC CTAAACATGA AAAACATTCT ATCTATTCTT 
CATACTTACT CTTCTCTCAA TCATGTCTAC AAATGCCAGA ACAAAGAACA GTTCGTGGAA 
GTTATGGCTA GTGCTCTGAC TGGTTATCTT CACACTATTT CTTCTGAAAA CTTATTGGAT 
GCAGTATATT CATTTTGCTT GATGAATTAC TTTCCCCTGG CTCCTTTTAA TCAGCTTCTG 
CAAAAAGACA TCATCAGTGA GCTGCTGACA TCAGATGACA TGAAGAATGC TTACAAGCTG 
CATACTTTGG ATACTTGTCT AAAACTTGAT GATACTGTCT ATCTGAGGGA CATAGCCTTG 
TCACTCCCAC AGCTGCCGCG GGAGCTGCCA TCGTCACATA CAAATGCAAA GGTGGCAGAG 
GTGCTGAGCA GCCTTCTGGG AGGTGAAGGA CACTTCTCAA AGGATGTGCA CTTGCCACAC 
AATTATCATA TTGATTTTGA AATCAGAATG GACACTAACA GGAATCAAGT GCTACCACTT 
TCTGATGTGG ATACAACTTC TGCTACAGAT ATTCAAAGAG TAGCTGTGCT ATGTGTTTCC 
AGATCTGCTT ATTGTTTGGG TTCAAGCCAC CCCAGAGGAT TCCTTGCTAT GAAAATGCGG 
CATTTGAATG CAATGGGTTT TCATGTGATC TTGGTCAATA ACTGGGAGAT GGACAAACTA 
GAGATGGAAG ATGCAGTCAC ATTTTTGAAG ACTAAAATCT ATTCAGTAGA AGCTCTTCCT 
GTTGCTGCTG TAAATGTGCA AAGCACACAA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_055744.2
CDS	318..2450
Translation	MLTTLKPFGSVSVESKMNNKAGSFFWNLRQFSTLVSTSRTMRLCCLGLCKPKIVHSNWNILNNFHNRMQSTDIIRYLFQDAFIFKSDVGFQTKGISTLTALRIERLLYAKRLFFDSKQSLVPVDKSDDELKKVNLNHEVSNEDVLTKETKPNRISSRKLSEECNSLSDVLDAFSKAPTFPSSNYFTAMWTIAKRLSDDQKRFEKRLMFSHPAFNQLCEHMMREAKIMQYKYLLFSLHAIVKLGIPQNTILVQTLLRVTQERINECDEICLSVLSTVLEAMEPCKNVHVLRTGFRILVDQQVWKIEDVFTLQVVMKCIGKDAPIALKRKLEMKALRELDRFSVLNSQHMFEVLAAMNHRSLILLDECSKVVLDNIHGCPLRIMINILQSCKDLQYHNLDLFKGLADYVAATFDIWKFRKVLFILILFENLGFRPVGLMDLFMKRIVEDPESLNMKNILSILHTYSSLNHVYKCQNKEQFVEVMASALTGYLHTISSENLLDAVYSFCLMNYFPLAPFNQLLQKDIISELLTSDDMKNAYKLHTLDTCLKLDDTVYLRDIALSLPQLPRELPSSHTNAKVAEVLSSLLGGEGHFSKDVHLPHNYHIDFEIRMDTNRNQVLPLSDVDTTSATDIQRVAVLCVSRSAYCLGSSHPRGFLAMKMRHLNAMGFHVILVNNWEMDKLEMEDAVTFLKTKIYSVEALPVAAVNVQSTQ

Target ORF information:

RefSeq Version	NM_014929.3
Organism	Homo sapiens(human)
Definition	Homo sapiens FAST kinase domains 2 (FASTKD2), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_014929.3

ORF Insert Sequence:

ATGTTGACAA CTTTGAAGCC ATTTGGAAGT GTTTCAGTGG AGAGCAAAAT GAATAACAAA 
GCGGGCTCCT TTTTCTGGAA CCTTAGACAA TTCAGTACAT TAGTTTCAAC AAGCAGAACT 
ATGAGGCTAT GTTGTTTGGG ACTTTGCAAA CCAAAAATAG TTCATTCAAA CTGGAACATT 
TTAAATAACT TTCATAACAG AATGCAATCA ACTGATATCA TTAGATATCT CTTTCAGGAT 
GCATTCATTT TTAAATCAGA TGTTGGCTTT CAAACAAAGG GCATAAGCAC TCTAACAGCC 
CTTAGAATTG AAAGACTACT TTATGCTAAA AGACTGTTTT TTGACTCAAA GCAGTCTCTT 
GTCCCTGTTG ATAAATCTGA TGATGAATTG AAGAAAGTAA ACCTTAATCA TGAAGTCTCC 
AATGAAGATG TTCTTACCAA GGAAACAAAA CCAAACCGTA TCAGCAGTAG AAAACTGTCT 
GAGGAATGTA ATTCCCTGAG TGATGTGTTA GATGCATTTT CAAAAGCGCC CACATTTCCT 
AGTAGCAACT ATTTCACAGC AATGTGGACA ATTGCCAAAA GACTGTCTGA TGACCAGAAG 
CGCTTTGAAA AACGACTGAT GTTTAGCCAC CCTGCATTTA ATCAGCTCTG TGAACATATG 
ATGAGAGAAG CCAAGATCAT GCAGTATAAG TACCTACTGT TCAGTCTTCA CGCCATAGTG 
AAGCTTGGAA TCCCTCAGAA CACTATTTTG GTGCAGACTT TGCTGAGGGT GACCCAGGAA 
CGTATCAATG AGTGTGATGA GATATGCCTT TCAGTTTTGT CAACTGTTTT AGAGGCAATG 
GAACCATGCA AGAATGTTCA TGTTCTACGA ACGGGATTCA GAATACTAGT TGATCAGCAA 
GTTTGGAAAA TAGAAGATGT CTTCACATTA CAAGTTGTGA TGAAGTGTAT TGGAAAAGAT 
GCACCGATTG CTCTTAAGAG GAAACTGGAG ATGAAAGCCT TGAGGGAATT AGACAGATTT 
TCTGTTTTGA ATAGCCAACA CATGTTTGAA GTACTAGCTG CCATGAATCA CCGATCTCTT 
ATACTCCTGG ATGAATGCAG TAAGGTGGTC CTAGATAATA TCCATGGGTG TCCTTTAAGA 
ATAATGATCA ACATATTGCA GTCCTGCAAA GACCTCCAGT ACCATAATTT GGATCTCTTC 
AAGGGACTTG CAGATTATGT GGCTGCAACT TTCGACATCT GGAAGTTCAG AAAAGTTCTT 
TTTATCCTCA TTTTATTTGA AAACCTTGGC TTTCGACCTG TTGGTTTAAT GGACCTGTTT 
ATGAAGAGAA TAGTAGAGGA TCCTGAATCC CTAAACATGA AAAACATTCT ATCTATTCTT 
CATACTTACT CTTCTCTCAA TCATGTCTAC AAATGCCAGA ACAAAGAACA GTTCGTGGAA 
GTTATGGCTA GTGCTCTGAC TGGTTATCTT CACACTATTT CTTCTGAAAA CTTATTGGAT 
GCAGTATATT CATTTTGCTT GATGAATTAC TTTCCCCTGG CTCCTTTTAA TCAGCTTCTG 
CAAAAAGACA TCATCAGTGA GCTGCTGACA TCAGATGACA TGAAGAATGC TTACAAGCTG 
CATACTTTGG ATACTTGTCT AAAACTTGAT GATACTGTCT ATCTGAGGGA CATAGCCTTG 
TCACTCCCAC AGCTGCCGCG GGAGCTGCCA TCGTCACATA CAAATGCAAA GGTGGCAGAG 
GTGCTGAGCA GCCTTCTGGG AGGTGAAGGA CACTTCTCAA AGGATGTGCA CTTGCCACAC 
AATTATCATA TTGATTTTGA AATCAGAATG GACACTAACA GGAATCAAGT GCTACCACTT 
TCTGATGTGG ATACAACTTC TGCTACAGAT ATTCAAAGAG TAGCTGTGCT ATGTGTTTCC 
AGATCTGCTT ATTGTTTGGG TTCAAGCCAC CCCAGAGGAT TCCTTGCTAT GAAAATGCGG 
CATTTGAATG CAATGGGTTT TCATGTGATC TTGGTCAATA ACTGGGAGAT GGACAAACTA 
GAGATGGAAG ATGCAGTCAC ATTTTTGAAG ACTAAAATCT ATTCAGTAGA AGCTCTTCCT 
GTTGCTGCTG TAAATGTGCA AAGCACACAA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The FASTK family of proteins: emerging regulators of mitochondrial RNA biology.
Nucleic acids research45(19)10941-10947(2017 Nov)
Jourdain AA,Popow J,de la Fuente MA,Martinou JC,Anderson P,Simarro M

A Genome-wide CRISPR Death Screen Identifies Genes Essential for Oxidative Phosphorylation.
Cell metabolism24(6)875-885(2016 Dec)
Arroyo JD,Jourdain AA,Calvo SE,Ballarano CA,Doench JG,Root DE,Mootha VK

FASTKD2 is an RNA-binding protein required for mitochondrial RNA processing and translation.
RNA (New York, N.Y.)21(11)1873-84(2015 Nov)
Popow J,Alleaume AM,Curk T,Schwarzl T,Sauer S,Hentze MW

Mitochondrial RNA Granules Are Centers for Posttranscriptional RNA Processing and Ribosome Biogenesis.
Cell reports(2015 Feb)
Antonicka H,Shoubridge EA

Fas Activated Serine-Threonine Kinase Domains 2 (FASTKD2) mediates apoptosis of breast and prostate cancer cells through its novel FAST2 domain.
BMC cancer14852(2014 Nov)
Das S,Yeung KT,Mahajan MA,Samuels HH

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FASTKD2 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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