CHSY1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	CHSY1
Entrez Gene ID	22856
Full Name	chondroitin sulfate synthase 1
Synonyms	CHSY,CSS1,ChSy-1,TPBS
General protein information	Preferred Names chondroitin sulfate synthase 1 Names chondroitin sulfate synthase 1 N-acetylgalactosaminyl-proteoglycan 3-beta-glucuronosyltransferase 1 N-acetylgalactosaminyltransferase II carbohydrate synthase 1 chondroitin glucuronyltransferase 1 chondroitin glucuronyltransferase II chondroitin synthase 1 glucuronosyl-N-acetylgalactosaminyl-proteoglycan 4-beta-N-acetylgalactosaminyltransferase 1
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 15 Map Location: 15q26.3
Summary	This gene encodes a member of the chondroitin N-acetylgalactosaminyltransferase family. These enzymes possess dual glucuronyltransferase and galactosaminyltransferase activity and play critical roles in the biosynthesis of chondroitin sulfate, a glycosaminoglycan involved in many biological processes including cell proliferation and morphogenesis. Decreased expression of this gene may play a role in colorectal cancer, and mutations in this gene are a cause of temtamy preaxial brachydactyly syndrome. [provided by RefSeq, Dec 2011].
Disorder MIM:	608183
Disorder Html:	Temtamy preaxial brachydactyly syndrome, 605282 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_014918.5	NP_055733.2	chondroitin sulfate synthase 1 precursor
XM_024449873.1	XP_024305641.1	chondroitin sulfate synthase 1 isoform X2
XM_006720435.3	XP_006720498.1	chondroitin sulfate synthase 1 isoform X3
NM_014918.4	NP_055733.2	chondroitin sulfate synthase 1 precursor
XM_011521364.2	XP_011519666.1	chondroitin sulfate synthase 1 isoform X1
XM_017022011.1	XP_016877500.1	chondroitin sulfate synthase 1 isoform X3

Pathways from BioSystems

Homologies

	CHSY1	XP_001084554.2
Rattus norvegicus (Norway rat)	Chsy1	NP_001099738.1
Homo sapiens (human)	CHSY1	NP_055733.2
Gallus gallus (chicken)	CHSY1	XP_413889.3
Drosophila melanogaster (fruit fly)	CG9220	NP_996440.2
Canis lupus familiaris (dog)	CHSY1	XP_545821.3
Danio rerio (zebrafish)	chsy1	NP_997843.1
Bos taurus (cattle)	CHSY1	NP_001178086.1
Mus musculus (house mouse)	Chsy1	NP_001074632.1
Xenopus tropicalis (tropical clawed frog)	chsy1	NP_001090724.1
Pan troglodytes (chimpanzee)	CHSY1	XP_523172.4
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001010	XP_309203.5

Gene Ontology
Bibliography

Related articles in PubMed

CHSY1 promotes aggressive phenotypes of hepatocellular carcinoma cells via activation of the hedgehog signaling pathway.
Liu CH, Lan CT, Chou JF, Tseng TJ, Liao WC
Cancer letters403280-288(2017 Sep)

Chondroitin sulfate synthase 1 expression is associated with malignant potential of soft tissue sarcomas with myxoid substance.
Momose T, Yoshimura Y, Harumiya S, Isobe K, Kito M, Fukushima M, Kato H, Nakayama J
Human pathology5015-23(2016 Apr)

A novel CHSY1 gene mutation underlies Temtamy preaxial brachydactyly syndrome in a Pakistani family.
Sher G, Naeem M
European journal of medical genetics57(1)21-4(2014 Jan)

Common variants in left/right asymmetry genes and pathways are associated with relative hand skill.
Brandler WM, Morris AP, Evans DM, Scerri TS, Kemp JP, Timpson NJ, St Pourcain B, Smith GD, Ring SM, Stein J, Monaco AP, Talcott JB, Fisher SE, Webber C, Paracchini S
PLoS genetics9(9)e1003751(2013)

Chondroitin synthases I, II, III and chondroitin sulfate glucuronyltransferase expression in colorectal cancer.
Kalathas D, Theocharis DA, Bounias D, Kyriakopoulou D, Papageorgakopoulou N, Stavropoulos MS, Vynios DH
Molecular medicine reports4(2)363-8(2011 Mar-Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

CHSY1 overexpression in HCC contributes to the malignant behavior of hepatocellular carcinoma cells via activation of the hedgehog signaling pathway.
Title: CHSY1 promotes aggressive phenotypes of hepatocellular carcinoma cells via activation of the hedgehog signaling pathway.

CHSY1 expression is closely associated with malignant potential of soft tissue sarcomas with myxoid substance.
Title: Chondroitin sulfate synthase 1 expression is associated with malignant potential of soft tissue sarcomas with myxoid substance.

A novel missense mutation (c.1897 G > A) in the CHSY1 gene in two Temtamy preaxial brachydactyly syndrome patients from a consanguineous Pakistani family.
Title: A novel CHSY1 gene mutation underlies Temtamy preaxial brachydactyly syndrome in a Pakistani family.

elongation of chondroitin sulfate chains may be tightly regulated by the cooperative expression of chondroitin synthase-1 and chondroitin N-acetylgalactosaminyltransferase-1 in peripheral neurons and peripheral neuropathies
Title: A chondroitin synthase-1 (ChSy-1) missense mutation in a patient with neuropathy impairs the elongation of chondroitin sulfate chains initiated by chondroitin N-acetylgalactosaminyltransferase-1.

The present study focused on the expression of chondroitin-synthesizing enzymes in colorectal cancer.
Title: Chondroitin synthases I, II, III and chondroitin sulfate glucuronyltransferase expression in colorectal cancer.

The following CHSY1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CHSY1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu48170	XM_017022011.1 Latest version!	Homo sapiens chondroitin sulfate synthase 1 (CHSY1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu48170	XM_006720435.3 Latest version!	Homo sapiens chondroitin sulfate synthase 1 (CHSY1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu103996	XM_024449873.1 Latest version!	Homo sapiens chondroitin sulfate synthase 1 (CHSY1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OHu22488	NM_014918.5 Latest version!	Homo sapiens chondroitin sulfate synthase 1 (CHSY1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$265.30 $379.00
OHu64998	XM_011521364.2 Latest version!	Homo sapiens chondroitin sulfate synthase 1 (CHSY1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$391.30 $559.00
View Old Accession Versions >>
OHu22488	NM_014918.4	Homo sapiens chondroitin sulfate synthase 1 (CHSY1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$265.30 $379.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CHSY1 ( XM_006720435.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_006720498.1 Homo sapiens chondroitin sulfate synthase 1 (CHSY1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHu48170
Clone ID Related Accession (Same CDS sequence)	XM_006720435.3 , XM_017022011.1
Accession Version	XM_006720435.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1593bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	chondroitin sulfate synthase 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006720435.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGCAGC TTTTTTATGA GAATTACGAG CAGAACAAAA AGGGGTACAT TAGAGATCTC 
CATAACAGTA AAATTCACCA AGCTATCACA TTACACCCCA ACAAAAACCC ACCCTACCAG 
TACAGGCTCC ACAGCTACAT GCTGAGCCGC AAGATATCCG AGCTCCGCCA TCGCACAATA 
CAGCTGCACC GCGAAATTGT CCTGATGAGC AAATACAGCA ACACAGAAAT TCATAAAGAG 
GACCTCCAGC TGGGAATCCC TCCCTCCTTC ATGAGGTTTC AGCCCCGCCA GCGAGAGGAG 
ATTCTGGAAT GGGAGTTTCT GACTGGAAAA TACTTGTATT CGGCAGTTGA CGGCCAGCCC 
CCTCGAAGAG GAATGGACTC CGCCCAGAGG GAAGCCTTGG ACGACATTGT CATGCAGGTC 
ATGGAGATGA TCAATGCCAA CGCCAAGACC AGAGGGCGCA TCATTGACTT CAAAGAGATC 
CAGTACGGCT ACCGCCGGGT GAACCCCATG TATGGGGCTG AGTACATCCT GGACCTGCTG 
CTTCTGTACA AAAAGCACAA AGGGAAGAAA ATGACGGTCC CTGTGAGGAG GCACGCGTAT 
TTACAGCAGA CTTTCAGCAA AATCCAGTTT GTGGAGCATG AGGAGCTGGA TGCACAAGAG 
TTGGCCAAGA GAATCAATCA GGAATCTGGA TCCTTGTCCT TTCTCTCAAA CTCCCTGAAG 
AAGCTCGTCC CCTTTCAGCT CCCTGGGTCG AAGAGTGAGC ACAAAGAACC CAAAGATAAA 
AAGATAAACA TACTGATTCC TTTGTCTGGG CGTTTCGACA TGTTTGTGAG ATTTATGGGA 
AACTTTGAGA AGACGTGTCT TATCCCCAAT CAGAACGTCA AGCTCGTGGT TCTGCTTTTC 
AATTCTGACT CCAACCCTGA CAAGGCCAAA CAAGTTGAAC TGATGAGAGA TTACCGCATT 
AAGTACCCTA AAGCCGACAT GCAGATTTTG CCTGTGTCTG GAGAGTTTTC AAGAGCCCTG 
GCCCTGGAAG TAGGATCCTC CCAGTTTAAC AATGAATCTT TGCTCTTCTT CTGCGACGTC 
GACCTCGTGT TTACTACAGA ATTCCTTCAG CGATGTCGAG CAAATACAGT TCTGGGCCAA 
CAAATATATT TTCCAATCAT CTTCAGCCAG TATGACCCAA AGATTGTTTA TAGTGGGAAA 
GTTCCCAGTG ACAACCATTT TGCCTTTACT CAGAAAACTG GCTTCTGGAG AAACTATGGG 
TTTGGCATCA CGTGTATTTA TAAGGGAGAT CTTGTCCGAG TGGGTGGCTT TGATGTTTCC 
ATCCAAGGCT GGGGGCTGGA GGATGTGGAC CTTTTCAACA AGGTTGTCCA GGCAGGTTTG 
AAGACGTTTA GGAGCCAGGA AGTAGGAGTA GTCCACGTCC ACCATCCTGT CTTTTGTGAT 
CCCAATCTTG ACCCCAAACA GTACAAAATG TGCTTGGGGT CCAAAGCATC GACCTATGGG 
TCCACCCAGC AGCTGGCTGA GATGTGGCTG GAAAAAAATG ATCCAAGTTA CAGTAAAAGC 
AGCAATAATA ATGGCTCAGT GAGGACAGCC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006720498.1
CDS	575..2167
Translation	MQQLFYENYEQNKKGYIRDLHNSKIHQAITLHPNKNPPYQYRLHSYMLSRKISELRHRTIQLHREIVLMSKYSNTEIHKEDLQLGIPPSFMRFQPRQREEILEWEFLTGKYLYSAVDGQPPRRGMDSAQREALDDIVMQVMEMINANAKTRGRIIDFKEIQYGYRRVNPMYGAEYILDLLLLYKKHKGKKMTVPVRRHAYLQQTFSKIQFVEHEELDAQELAKRINQESGSLSFLSNSLKKLVPFQLPGSKSEHKEPKDKKINILIPLSGRFDMFVRFMGNFEKTCLIPNQNVKLVVLLFNSDSNPDKAKQVELMRDYRIKYPKADMQILPVSGEFSRALALEVGSSQFNNESLLFFCDVDLVFTTEFLQRCRANTVLGQQIYFPIIFSQYDPKIVYSGKVPSDNHFAFTQKTGFWRNYGFGITCIYKGDLVRVGGFDVSIQGWGLEDVDLFNKVVQAGLKTFRSQEVGVVHVHHPVFCDPNLDPKQYKMCLGSKASTYGSTQQLAEMWLEKNDPSYSKSSNNNGSVRTA

Target ORF information:

RefSeq Version	XM_006720435.3
Organism	Homo sapiens(human)
Definition	Homo sapiens chondroitin sulfate synthase 1 (CHSY1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006720435.3

ORF Insert Sequence:

ATGCAGCAGC TTTTTTATGA GAATTACGAG CAGAACAAAA AGGGGTACAT TAGAGATCTC 
CATAACAGTA AAATTCACCA AGCTATCACA TTACACCCCA ACAAAAACCC ACCCTACCAG 
TACAGGCTCC ACAGCTACAT GCTGAGCCGC AAGATATCCG AGCTCCGCCA TCGCACAATA 
CAGCTGCACC GCGAAATTGT CCTGATGAGC AAATACAGCA ACACAGAAAT TCATAAAGAG 
GACCTCCAGC TGGGAATCCC TCCCTCCTTC ATGAGGTTTC AGCCCCGCCA GCGAGAGGAG 
ATTCTGGAAT GGGAGTTTCT GACTGGAAAA TACTTGTATT CGGCAGTTGA CGGCCAGCCC 
CCTCGAAGAG GAATGGACTC CGCCCAGAGG GAAGCCTTGG ACGACATTGT CATGCAGGTC 
ATGGAGATGA TCAATGCCAA CGCCAAGACC AGAGGGCGCA TCATTGACTT CAAAGAGATC 
CAGTACGGCT ACCGCCGGGT GAACCCCATG TATGGGGCTG AGTACATCCT GGACCTGCTG 
CTTCTGTACA AAAAGCACAA AGGGAAGAAA ATGACGGTCC CTGTGAGGAG GCACGCGTAT 
TTACAGCAGA CTTTCAGCAA AATCCAGTTT GTGGAGCATG AGGAGCTGGA TGCACAAGAG 
TTGGCCAAGA GAATCAATCA GGAATCTGGA TCCTTGTCCT TTCTCTCAAA CTCCCTGAAG 
AAGCTCGTCC CCTTTCAGCT CCCTGGGTCG AAGAGTGAGC ACAAAGAACC CAAAGATAAA 
AAGATAAACA TACTGATTCC TTTGTCTGGG CGTTTCGACA TGTTTGTGAG ATTTATGGGA 
AACTTTGAGA AGACGTGTCT TATCCCCAAT CAGAACGTCA AGCTCGTGGT TCTGCTTTTC 
AATTCTGACT CCAACCCTGA CAAGGCCAAA CAAGTTGAAC TGATGAGAGA TTACCGCATT 
AAGTACCCTA AAGCCGACAT GCAGATTTTG CCTGTGTCTG GAGAGTTTTC AAGAGCCCTG 
GCCCTGGAAG TAGGATCCTC CCAGTTTAAC AATGAATCTT TGCTCTTCTT CTGCGACGTC 
GACCTCGTGT TTACTACAGA ATTCCTTCAG CGATGTCGAG CAAATACAGT TCTGGGCCAA 
CAAATATATT TTCCAATCAT CTTCAGCCAG TATGACCCAA AGATTGTTTA TAGTGGGAAA 
GTTCCCAGTG ACAACCATTT TGCCTTTACT CAGAAAACTG GCTTCTGGAG AAACTATGGG 
TTTGGCATCA CGTGTATTTA TAAGGGAGAT CTTGTCCGAG TGGGTGGCTT TGATGTTTCC 
ATCCAAGGCT GGGGGCTGGA GGATGTGGAC CTTTTCAACA AGGTTGTCCA GGCAGGTTTG 
AAGACGTTTA GGAGCCAGGA AGTAGGAGTA GTCCACGTCC ACCATCCTGT CTTTTGTGAT 
CCCAATCTTG ACCCCAAACA GTACAAAATG TGCTTGGGGT CCAAAGCATC GACCTATGGG 
TCCACCCAGC AGCTGGCTGA GATGTGGCTG GAAAAAAATG ATCCAAGTTA CAGTAAAAGC 
AGCAATAATA ATGGCTCAGT GAGGACAGCC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CHSY1 ( XM_017022011.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016877500.1 Homo sapiens chondroitin sulfate synthase 1 (CHSY1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OHu48170
Clone ID Related Accession (Same CDS sequence)	XM_006720435.3 , XM_017022011.1
Accession Version	XM_017022011.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1593bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	chondroitin sulfate synthase 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGCAGC TTTTTTATGA GAATTACGAG CAGAACAAAA AGGGGTACAT TAGAGATCTC 
CATAACAGTA AAATTCACCA AGCTATCACA TTACACCCCA ACAAAAACCC ACCCTACCAG 
TACAGGCTCC ACAGCTACAT GCTGAGCCGC AAGATATCCG AGCTCCGCCA TCGCACAATA 
CAGCTGCACC GCGAAATTGT CCTGATGAGC AAATACAGCA ACACAGAAAT TCATAAAGAG 
GACCTCCAGC TGGGAATCCC TCCCTCCTTC ATGAGGTTTC AGCCCCGCCA GCGAGAGGAG 
ATTCTGGAAT GGGAGTTTCT GACTGGAAAA TACTTGTATT CGGCAGTTGA CGGCCAGCCC 
CCTCGAAGAG GAATGGACTC CGCCCAGAGG GAAGCCTTGG ACGACATTGT CATGCAGGTC 
ATGGAGATGA TCAATGCCAA CGCCAAGACC AGAGGGCGCA TCATTGACTT CAAAGAGATC 
CAGTACGGCT ACCGCCGGGT GAACCCCATG TATGGGGCTG AGTACATCCT GGACCTGCTG 
CTTCTGTACA AAAAGCACAA AGGGAAGAAA ATGACGGTCC CTGTGAGGAG GCACGCGTAT 
TTACAGCAGA CTTTCAGCAA AATCCAGTTT GTGGAGCATG AGGAGCTGGA TGCACAAGAG 
TTGGCCAAGA GAATCAATCA GGAATCTGGA TCCTTGTCCT TTCTCTCAAA CTCCCTGAAG 
AAGCTCGTCC CCTTTCAGCT CCCTGGGTCG AAGAGTGAGC ACAAAGAACC CAAAGATAAA 
AAGATAAACA TACTGATTCC TTTGTCTGGG CGTTTCGACA TGTTTGTGAG ATTTATGGGA 
AACTTTGAGA AGACGTGTCT TATCCCCAAT CAGAACGTCA AGCTCGTGGT TCTGCTTTTC 
AATTCTGACT CCAACCCTGA CAAGGCCAAA CAAGTTGAAC TGATGAGAGA TTACCGCATT 
AAGTACCCTA AAGCCGACAT GCAGATTTTG CCTGTGTCTG GAGAGTTTTC AAGAGCCCTG 
GCCCTGGAAG TAGGATCCTC CCAGTTTAAC AATGAATCTT TGCTCTTCTT CTGCGACGTC 
GACCTCGTGT TTACTACAGA ATTCCTTCAG CGATGTCGAG CAAATACAGT TCTGGGCCAA 
CAAATATATT TTCCAATCAT CTTCAGCCAG TATGACCCAA AGATTGTTTA TAGTGGGAAA 
GTTCCCAGTG ACAACCATTT TGCCTTTACT CAGAAAACTG GCTTCTGGAG AAACTATGGG 
TTTGGCATCA CGTGTATTTA TAAGGGAGAT CTTGTCCGAG TGGGTGGCTT TGATGTTTCC 
ATCCAAGGCT GGGGGCTGGA GGATGTGGAC CTTTTCAACA AGGTTGTCCA GGCAGGTTTG 
AAGACGTTTA GGAGCCAGGA AGTAGGAGTA GTCCACGTCC ACCATCCTGT CTTTTGTGAT 
CCCAATCTTG ACCCCAAACA GTACAAAATG TGCTTGGGGT CCAAAGCATC GACCTATGGG 
TCCACCCAGC AGCTGGCTGA GATGTGGCTG GAAAAAAATG ATCCAAGTTA CAGTAAAAGC 
AGCAATAATA ATGGCTCAGT GAGGACAGCC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016877500.1
CDS	349..1941
Translation	MQQLFYENYEQNKKGYIRDLHNSKIHQAITLHPNKNPPYQYRLHSYMLSRKISELRHRTIQLHREIVLMSKYSNTEIHKEDLQLGIPPSFMRFQPRQREEILEWEFLTGKYLYSAVDGQPPRRGMDSAQREALDDIVMQVMEMINANAKTRGRIIDFKEIQYGYRRVNPMYGAEYILDLLLLYKKHKGKKMTVPVRRHAYLQQTFSKIQFVEHEELDAQELAKRINQESGSLSFLSNSLKKLVPFQLPGSKSEHKEPKDKKINILIPLSGRFDMFVRFMGNFEKTCLIPNQNVKLVVLLFNSDSNPDKAKQVELMRDYRIKYPKADMQILPVSGEFSRALALEVGSSQFNNESLLFFCDVDLVFTTEFLQRCRANTVLGQQIYFPIIFSQYDPKIVYSGKVPSDNHFAFTQKTGFWRNYGFGITCIYKGDLVRVGGFDVSIQGWGLEDVDLFNKVVQAGLKTFRSQEVGVVHVHHPVFCDPNLDPKQYKMCLGSKASTYGSTQQLAEMWLEKNDPSYSKSSNNNGSVRTA

Target ORF information:

RefSeq Version	XM_017022011.1
Organism	Homo sapiens(human)
Definition	Homo sapiens chondroitin sulfate synthase 1 (CHSY1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017022011.1

ORF Insert Sequence:

ATGCAGCAGC TTTTTTATGA GAATTACGAG CAGAACAAAA AGGGGTACAT TAGAGATCTC 
CATAACAGTA AAATTCACCA AGCTATCACA TTACACCCCA ACAAAAACCC ACCCTACCAG 
TACAGGCTCC ACAGCTACAT GCTGAGCCGC AAGATATCCG AGCTCCGCCA TCGCACAATA 
CAGCTGCACC GCGAAATTGT CCTGATGAGC AAATACAGCA ACACAGAAAT TCATAAAGAG 
GACCTCCAGC TGGGAATCCC TCCCTCCTTC ATGAGGTTTC AGCCCCGCCA GCGAGAGGAG 
ATTCTGGAAT GGGAGTTTCT GACTGGAAAA TACTTGTATT CGGCAGTTGA CGGCCAGCCC 
CCTCGAAGAG GAATGGACTC CGCCCAGAGG GAAGCCTTGG ACGACATTGT CATGCAGGTC 
ATGGAGATGA TCAATGCCAA CGCCAAGACC AGAGGGCGCA TCATTGACTT CAAAGAGATC 
CAGTACGGCT ACCGCCGGGT GAACCCCATG TATGGGGCTG AGTACATCCT GGACCTGCTG 
CTTCTGTACA AAAAGCACAA AGGGAAGAAA ATGACGGTCC CTGTGAGGAG GCACGCGTAT 
TTACAGCAGA CTTTCAGCAA AATCCAGTTT GTGGAGCATG AGGAGCTGGA TGCACAAGAG 
TTGGCCAAGA GAATCAATCA GGAATCTGGA TCCTTGTCCT TTCTCTCAAA CTCCCTGAAG 
AAGCTCGTCC CCTTTCAGCT CCCTGGGTCG AAGAGTGAGC ACAAAGAACC CAAAGATAAA 
AAGATAAACA TACTGATTCC TTTGTCTGGG CGTTTCGACA TGTTTGTGAG ATTTATGGGA 
AACTTTGAGA AGACGTGTCT TATCCCCAAT CAGAACGTCA AGCTCGTGGT TCTGCTTTTC 
AATTCTGACT CCAACCCTGA CAAGGCCAAA CAAGTTGAAC TGATGAGAGA TTACCGCATT 
AAGTACCCTA AAGCCGACAT GCAGATTTTG CCTGTGTCTG GAGAGTTTTC AAGAGCCCTG 
GCCCTGGAAG TAGGATCCTC CCAGTTTAAC AATGAATCTT TGCTCTTCTT CTGCGACGTC 
GACCTCGTGT TTACTACAGA ATTCCTTCAG CGATGTCGAG CAAATACAGT TCTGGGCCAA 
CAAATATATT TTCCAATCAT CTTCAGCCAG TATGACCCAA AGATTGTTTA TAGTGGGAAA 
GTTCCCAGTG ACAACCATTT TGCCTTTACT CAGAAAACTG GCTTCTGGAG AAACTATGGG 
TTTGGCATCA CGTGTATTTA TAAGGGAGAT CTTGTCCGAG TGGGTGGCTT TGATGTTTCC 
ATCCAAGGCT GGGGGCTGGA GGATGTGGAC CTTTTCAACA AGGTTGTCCA GGCAGGTTTG 
AAGACGTTTA GGAGCCAGGA AGTAGGAGTA GTCCACGTCC ACCATCCTGT CTTTTGTGAT 
CCCAATCTTG ACCCCAAACA GTACAAAATG TGCTTGGGGT CCAAAGCATC GACCTATGGG 
TCCACCCAGC AGCTGGCTGA GATGTGGCTG GAAAAAAATG ATCCAAGTTA CAGTAAAAGC 
AGCAATAATA ATGGCTCAGT GAGGACAGCC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CHSY1 ( XM_024449873.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024305641.1 Homo sapiens chondroitin sulfate synthase 1 (CHSY1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu103996
Clone ID Related Accession (Same CDS sequence)	XM_024449873.1
Accession Version	XM_024449873.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1788bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	chondroitin sulfate synthase 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGGTG CTCCATGGAG GGCGTTCACA GGGAAGGGGG ATGGAGGGTG CTCCATGGAG 
GGCGTTCACA GGGAAGGAGG ATGGAGGGTG CTCCATGGAG AGTGTTCACA GGGAAGGAGG 
ATGGAGGGTG CTCCATGGAG GTTGTTCACA CTCAGAGCAA GGGCTGGCCT GGAGGTCCCA 
TGGCCCTGGA GCCTGATGCA GCAGCTTTTT TATGAGAATT ACGAGCAGAA CAAAAAGGGG 
TACATTAGAG ATCTCCATAA CAGTAAAATT CACCAAGCTA TCACATTACA CCCCAACAAA 
AACCCACCCT ACCAGTACAG GCTCCACAGC TACATGCTGA GCCGCAAGAT ATCCGAGCTC 
CGCCATCGCA CAATACAGCT GCACCGCGAA ATTGTCCTGA TGAGCAAATA CAGCAACACA 
GAAATTCATA AAGAGGACCT CCAGCTGGGA ATCCCTCCCT CCTTCATGAG GTTTCAGCCC 
CGCCAGCGAG AGGAGATTCT GGAATGGGAG TTTCTGACTG GAAAATACTT GTATTCGGCA 
GTTGACGGCC AGCCCCCTCG AAGAGGAATG GACTCCGCCC AGAGGGAAGC CTTGGACGAC 
ATTGTCATGC AGGTCATGGA GATGATCAAT GCCAACGCCA AGACCAGAGG GCGCATCATT 
GACTTCAAAG AGATCCAGTA CGGCTACCGC CGGGTGAACC CCATGTATGG GGCTGAGTAC 
ATCCTGGACC TGCTGCTTCT GTACAAAAAG CACAAAGGGA AGAAAATGAC GGTCCCTGTG 
AGGAGGCACG CGTATTTACA GCAGACTTTC AGCAAAATCC AGTTTGTGGA GCATGAGGAG 
CTGGATGCAC AAGAGTTGGC CAAGAGAATC AATCAGGAAT CTGGATCCTT GTCCTTTCTC 
TCAAACTCCC TGAAGAAGCT CGTCCCCTTT CAGCTCCCTG GGTCGAAGAG TGAGCACAAA 
GAACCCAAAG ATAAAAAGAT AAACATACTG ATTCCTTTGT CTGGGCGTTT CGACATGTTT 
GTGAGATTTA TGGGAAACTT TGAGAAGACG TGTCTTATCC CCAATCAGAA CGTCAAGCTC 
GTGGTTCTGC TTTTCAATTC TGACTCCAAC CCTGACAAGG CCAAACAAGT TGAACTGATG 
AGAGATTACC GCATTAAGTA CCCTAAAGCC GACATGCAGA TTTTGCCTGT GTCTGGAGAG 
TTTTCAAGAG CCCTGGCCCT GGAAGTAGGA TCCTCCCAGT TTAACAATGA ATCTTTGCTC 
TTCTTCTGCG ACGTCGACCT CGTGTTTACT ACAGAATTCC TTCAGCGATG TCGAGCAAAT 
ACAGTTCTGG GCCAACAAAT ATATTTTCCA ATCATCTTCA GCCAGTATGA CCCAAAGATT 
GTTTATAGTG GGAAAGTTCC CAGTGACAAC CATTTTGCCT TTACTCAGAA AACTGGCTTC 
TGGAGAAACT ATGGGTTTGG CATCACGTGT ATTTATAAGG GAGATCTTGT CCGAGTGGGT 
GGCTTTGATG TTTCCATCCA AGGCTGGGGG CTGGAGGATG TGGACCTTTT CAACAAGGTT 
GTCCAGGCAG GTTTGAAGAC GTTTAGGAGC CAGGAAGTAG GAGTAGTCCA CGTCCACCAT 
CCTGTCTTTT GTGATCCCAA TCTTGACCCC AAACAGTACA AAATGTGCTT GGGGTCCAAA 
GCATCGACCT ATGGGTCCAC CCAGCAGCTG GCTGAGATGT GGCTGGAAAA AAATGATCCA 
AGTTACAGTA AAAGCAGCAA TAATAATGGC TCAGTGAGGA CAGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024305641.1
CDS	16..1803
Translation	MEGAPWRAFTGKGDGGCSMEGVHREGGWRVLHGECSQGRRMEGAPWRLFTLRARAGLEVPWPWSLMQQLFYENYEQNKKGYIRDLHNSKIHQAITLHPNKNPPYQYRLHSYMLSRKISELRHRTIQLHREIVLMSKYSNTEIHKEDLQLGIPPSFMRFQPRQREEILEWEFLTGKYLYSAVDGQPPRRGMDSAQREALDDIVMQVMEMINANAKTRGRIIDFKEIQYGYRRVNPMYGAEYILDLLLLYKKHKGKKMTVPVRRHAYLQQTFSKIQFVEHEELDAQELAKRINQESGSLSFLSNSLKKLVPFQLPGSKSEHKEPKDKKINILIPLSGRFDMFVRFMGNFEKTCLIPNQNVKLVVLLFNSDSNPDKAKQVELMRDYRIKYPKADMQILPVSGEFSRALALEVGSSQFNNESLLFFCDVDLVFTTEFLQRCRANTVLGQQIYFPIIFSQYDPKIVYSGKVPSDNHFAFTQKTGFWRNYGFGITCIYKGDLVRVGGFDVSIQGWGLEDVDLFNKVVQAGLKTFRSQEVGVVHVHHPVFCDPNLDPKQYKMCLGSKASTYGSTQQLAEMWLEKNDPSYSKSSNNNGSVRTA

Target ORF information:

RefSeq Version	XM_024449873.1
Organism	Homo sapiens(human)
Definition	Homo sapiens chondroitin sulfate synthase 1 (CHSY1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024449873.1

ORF Insert Sequence:

ATGGAGGGTG CTCCATGGAG GGCGTTCACA GGGAAGGGGG ATGGAGGGTG CTCCATGGAG 
GGCGTTCACA GGGAAGGAGG ATGGAGGGTG CTCCATGGAG AGTGTTCACA GGGAAGGAGG 
ATGGAGGGTG CTCCATGGAG GTTGTTCACA CTCAGAGCAA GGGCTGGCCT GGAGGTCCCA 
TGGCCCTGGA GCCTGATGCA GCAGCTTTTT TATGAGAATT ACGAGCAGAA CAAAAAGGGG 
TACATTAGAG ATCTCCATAA CAGTAAAATT CACCAAGCTA TCACATTACA CCCCAACAAA 
AACCCACCCT ACCAGTACAG GCTCCACAGC TACATGCTGA GCCGCAAGAT ATCCGAGCTC 
CGCCATCGCA CAATACAGCT GCACCGCGAA ATTGTCCTGA TGAGCAAATA CAGCAACACA 
GAAATTCATA AAGAGGACCT CCAGCTGGGA ATCCCTCCCT CCTTCATGAG GTTTCAGCCC 
CGCCAGCGAG AGGAGATTCT GGAATGGGAG TTTCTGACTG GAAAATACTT GTATTCGGCA 
GTTGACGGCC AGCCCCCTCG AAGAGGAATG GACTCCGCCC AGAGGGAAGC CTTGGACGAC 
ATTGTCATGC AGGTCATGGA GATGATCAAT GCCAACGCCA AGACCAGAGG GCGCATCATT 
GACTTCAAAG AGATCCAGTA CGGCTACCGC CGGGTGAACC CCATGTATGG GGCTGAGTAC 
ATCCTGGACC TGCTGCTTCT GTACAAAAAG CACAAAGGGA AGAAAATGAC GGTCCCTGTG 
AGGAGGCACG CGTATTTACA GCAGACTTTC AGCAAAATCC AGTTTGTGGA GCATGAGGAG 
CTGGATGCAC AAGAGTTGGC CAAGAGAATC AATCAGGAAT CTGGATCCTT GTCCTTTCTC 
TCAAACTCCC TGAAGAAGCT CGTCCCCTTT CAGCTCCCTG GGTCGAAGAG TGAGCACAAA 
GAACCCAAAG ATAAAAAGAT AAACATACTG ATTCCTTTGT CTGGGCGTTT CGACATGTTT 
GTGAGATTTA TGGGAAACTT TGAGAAGACG TGTCTTATCC CCAATCAGAA CGTCAAGCTC 
GTGGTTCTGC TTTTCAATTC TGACTCCAAC CCTGACAAGG CCAAACAAGT TGAACTGATG 
AGAGATTACC GCATTAAGTA CCCTAAAGCC GACATGCAGA TTTTGCCTGT GTCTGGAGAG 
TTTTCAAGAG CCCTGGCCCT GGAAGTAGGA TCCTCCCAGT TTAACAATGA ATCTTTGCTC 
TTCTTCTGCG ACGTCGACCT CGTGTTTACT ACAGAATTCC TTCAGCGATG TCGAGCAAAT 
ACAGTTCTGG GCCAACAAAT ATATTTTCCA ATCATCTTCA GCCAGTATGA CCCAAAGATT 
GTTTATAGTG GGAAAGTTCC CAGTGACAAC CATTTTGCCT TTACTCAGAA AACTGGCTTC 
TGGAGAAACT ATGGGTTTGG CATCACGTGT ATTTATAAGG GAGATCTTGT CCGAGTGGGT 
GGCTTTGATG TTTCCATCCA AGGCTGGGGG CTGGAGGATG TGGACCTTTT CAACAAGGTT 
GTCCAGGCAG GTTTGAAGAC GTTTAGGAGC CAGGAAGTAG GAGTAGTCCA CGTCCACCAT 
CCTGTCTTTT GTGATCCCAA TCTTGACCCC AAACAGTACA AAATGTGCTT GGGGTCCAAA 
GCATCGACCT ATGGGTCCAC CCAGCAGCTG GCTGAGATGT GGCTGGAAAA AAATGATCCA 
AGTTACAGTA AAAGCAGCAA TAATAATGGC TCAGTGAGGA CAGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CHSY1 ( NM_014918.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_055733.2 Homo sapiens chondroitin sulfate synthase 1 (CHSY1), mRNA.

Price & Availability↑

CloneID	OHu22488
Clone ID Related Accession (Same CDS sequence)	NM_014918.5 , NM_014918.4
Accession Version	NM_014918.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2409bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-21
Organism	Homo sapiens(human)
Product	chondroitin sulfate synthase 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC023024.6, AB023207.1, BC046247.1, AC019254.12 and BQ007796.1. This sequence is a reference standard in the RefSeqGene project. On Nov 23, 2018 this sequence version replaced NM_014918.4. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY358529.1, AB023207.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1966682, SAMEA1968540 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000254190.4/ ENSP00000254190.3 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCCGCGC GCGGCCGGCG CGCCTGGCTC AGCGTGCTGC TCGGGCTCGT CCTGGGCTTC 
GTGCTGGCCT CGCGGCTCGT CCTGCCCCGG GCTTCCGAGC TGAAGCGAGC GGGCCCACGG 
CGCCGCGCCA GCCCCGAGGG CTGCCGGTCC GGGCAGGCGG CGGCTTCCCA GGCCGGCGGG 
GCGCGCGGCG ATGCGCGCGG GGCGCAGCTC TGGCCGCCCG GCTCGGACCC AGATGGCGGC 
CCGCGCGACA GGAACTTTCT CTTCGTGGGA GTCATGACCG CCCAGAAATA CCTGCAGACT 
CGGGCCGTGG CCGCCTACAG AACATGGTCC AAGACAATTC CTGGGAAAGT TCAGTTCTTC 
TCAAGTGAGG GTTCTGACAC ATCTGTACCA ATTCCAGTAG TGCCACTACG GGGTGTGGAC 
GACTCCTACC CGCCCCAGAA GAAGTCCTTC ATGATGCTCA AGTACATGCA CGACCACTAC 
TTGGACAAGT ATGAATGGTT TATGAGAGCA GATGATGACG TGTACATCAA AGGAGACCGT 
CTGGAGAACT TCCTGAGGAG TTTGAACAGC AGCGAGCCCC TCTTTCTTGG GCAGACAGGC 
CTGGGCACCA CGGAAGAAAT GGGAAAACTG GCCCTGGAGC CTGGTGAGAA CTTCTGCATG 
GGGGGGCCTG GCGTGATCAT GAGCCGGGAG GTGCTTCGGA GAATGGTGCC GCACATTGGC 
AAGTGTCTCC GGGAGATGTA CACCACCCAT GAGGACGTGG AGGTGGGAAG GTGTGTCCGG 
AGGTTTGCAG GGGTGCAGTG TGTCTGGTCT TATGAGATGC AGCAGCTTTT TTATGAGAAT 
TACGAGCAGA ACAAAAAGGG GTACATTAGA GATCTCCATA ACAGTAAAAT TCACCAAGCT 
ATCACATTAC ACCCCAACAA AAACCCACCC TACCAGTACA GGCTCCACAG CTACATGCTG 
AGCCGCAAGA TATCCGAGCT CCGCCATCGC ACAATACAGC TGCACCGCGA AATTGTCCTG 
ATGAGCAAAT ACAGCAACAC AGAAATTCAT AAAGAGGACC TCCAGCTGGG AATCCCTCCC 
TCCTTCATGA GGTTTCAGCC CCGCCAGCGA GAGGAGATTC TGGAATGGGA GTTTCTGACT 
GGAAAATACT TGTATTCGGC AGTTGACGGC CAGCCCCCTC GAAGAGGAAT GGACTCCGCC 
CAGAGGGAAG CCTTGGACGA CATTGTCATG CAGGTCATGG AGATGATCAA TGCCAACGCC 
AAGACCAGAG GGCGCATCAT TGACTTCAAA GAGATCCAGT ACGGCTACCG CCGGGTGAAC 
CCCATGTATG GGGCTGAGTA CATCCTGGAC CTGCTGCTTC TGTACAAAAA GCACAAAGGG 
AAGAAAATGA CGGTCCCTGT GAGGAGGCAC GCGTATTTAC AGCAGACTTT CAGCAAAATC 
CAGTTTGTGG AGCATGAGGA GCTGGATGCA CAAGAGTTGG CCAAGAGAAT CAATCAGGAA 
TCTGGATCCT TGTCCTTTCT CTCAAACTCC CTGAAGAAGC TCGTCCCCTT TCAGCTCCCT 
GGGTCGAAGA GTGAGCACAA AGAACCCAAA GATAAAAAGA TAAACATACT GATTCCTTTG 
TCTGGGCGTT TCGACATGTT TGTGAGATTT ATGGGAAACT TTGAGAAGAC GTGTCTTATC 
CCCAATCAGA ACGTCAAGCT CGTGGTTCTG CTTTTCAATT CTGACTCCAA CCCTGACAAG 
GCCAAACAAG TTGAACTGAT GAGAGATTAC CGCATTAAGT ACCCTAAAGC CGACATGCAG 
ATTTTGCCTG TGTCTGGAGA GTTTTCAAGA GCCCTGGCCC TGGAAGTAGG ATCCTCCCAG 
TTTAACAATG AATCTTTGCT CTTCTTCTGC GACGTCGACC TCGTGTTTAC TACAGAATTC 
CTTCAGCGAT GTCGAGCAAA TACAGTTCTG GGCCAACAAA TATATTTTCC AATCATCTTC 
AGCCAGTATG ACCCAAAGAT TGTTTATAGT GGGAAAGTTC CCAGTGACAA CCATTTTGCC 
TTTACTCAGA AAACTGGCTT CTGGAGAAAC TATGGGTTTG GCATCACGTG TATTTATAAG 
GGAGATCTTG TCCGAGTGGG TGGCTTTGAT GTTTCCATCC AAGGCTGGGG GCTGGAGGAT 
GTGGACCTTT TCAACAAGGT TGTCCAGGCA GGTTTGAAGA CGTTTAGGAG CCAGGAAGTA 
GGAGTAGTCC ACGTCCACCA TCCTGTCTTT TGTGATCCCA ATCTTGACCC CAAACAGTAC 
AAAATGTGCT TGGGGTCCAA AGCATCGACC TATGGGTCCA CCCAGCAGCT GGCTGAGATG 
TGGCTGGAAA AAAATGATCC AAGTTACAGT AAAAGCAGCA ATAATAATGG CTCAGTGAGG 
ACAGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_055733.2
CDS	593..3001
Translation	MAARGRRAWLSVLLGLVLGFVLASRLVLPRASELKRAGPRRRASPEGCRSGQAAASQAGGARGDARGAQLWPPGSDPDGGPRDRNFLFVGVMTAQKYLQTRAVAAYRTWSKTIPGKVQFFSSEGSDTSVPIPVVPLRGVDDSYPPQKKSFMMLKYMHDHYLDKYEWFMRADDDVYIKGDRLENFLRSLNSSEPLFLGQTGLGTTEEMGKLALEPGENFCMGGPGVIMSREVLRRMVPHIGKCLREMYTTHEDVEVGRCVRRFAGVQCVWSYEMQQLFYENYEQNKKGYIRDLHNSKIHQAITLHPNKNPPYQYRLHSYMLSRKISELRHRTIQLHREIVLMSKYSNTEIHKEDLQLGIPPSFMRFQPRQREEILEWEFLTGKYLYSAVDGQPPRRGMDSAQREALDDIVMQVMEMINANAKTRGRIIDFKEIQYGYRRVNPMYGAEYILDLLLLYKKHKGKKMTVPVRRHAYLQQTFSKIQFVEHEELDAQELAKRINQESGSLSFLSNSLKKLVPFQLPGSKSEHKEPKDKKINILIPLSGRFDMFVRFMGNFEKTCLIPNQNVKLVVLLFNSDSNPDKAKQVELMRDYRIKYPKADMQILPVSGEFSRALALEVGSSQFNNESLLFFCDVDLVFTTEFLQRCRANTVLGQQIYFPIIFSQYDPKIVYSGKVPSDNHFAFTQKTGFWRNYGFGITCIYKGDLVRVGGFDVSIQGWGLEDVDLFNKVVQAGLKTFRSQEVGVVHVHHPVFCDPNLDPKQYKMCLGSKASTYGSTQQLAEMWLEKNDPSYSKSSNNNGSVRTA

Target ORF information:

RefSeq Version	NM_014918.5
Organism	Homo sapiens(human)
Definition	Homo sapiens chondroitin sulfate synthase 1 (CHSY1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_014918.5

ORF Insert Sequence:

ATGGCCGCGC GCGGCCGGCG CGCCTGGCTC AGCGTGCTGC TCGGGCTCGT CCTGGGCTTC 
GTGCTGGCCT CGCGGCTCGT CCTGCCCCGG GCTTCCGAGC TGAAGCGAGC GGGCCCACGG 
CGCCGCGCCA GCCCCGAGGG CTGCCGGTCC GGGCAGGCGG CGGCTTCCCA GGCCGGCGGG 
GCGCGCGGCG ATGCGCGCGG GGCGCAGCTC TGGCCGCCCG GCTCGGACCC AGATGGCGGC 
CCGCGCGACA GGAACTTTCT CTTCGTGGGA GTCATGACCG CCCAGAAATA CCTGCAGACT 
CGGGCCGTGG CCGCCTACAG AACATGGTCC AAGACAATTC CTGGGAAAGT TCAGTTCTTC 
TCAAGTGAGG GTTCTGACAC ATCTGTACCA ATTCCAGTAG TGCCACTACG GGGTGTGGAC 
GACTCCTACC CGCCCCAGAA GAAGTCCTTC ATGATGCTCA AGTACATGCA CGACCACTAC 
TTGGACAAGT ATGAATGGTT TATGAGAGCA GATGATGACG TGTACATCAA AGGAGACCGT 
CTGGAGAACT TCCTGAGGAG TTTGAACAGC AGCGAGCCCC TCTTTCTTGG GCAGACAGGC 
CTGGGCACCA CGGAAGAAAT GGGAAAACTG GCCCTGGAGC CTGGTGAGAA CTTCTGCATG 
GGGGGGCCTG GCGTGATCAT GAGCCGGGAG GTGCTTCGGA GAATGGTGCC GCACATTGGC 
AAGTGTCTCC GGGAGATGTA CACCACCCAT GAGGACGTGG AGGTGGGAAG GTGTGTCCGG 
AGGTTTGCAG GGGTGCAGTG TGTCTGGTCT TATGAGATGC AGCAGCTTTT TTATGAGAAT 
TACGAGCAGA ACAAAAAGGG GTACATTAGA GATCTCCATA ACAGTAAAAT TCACCAAGCT 
ATCACATTAC ACCCCAACAA AAACCCACCC TACCAGTACA GGCTCCACAG CTACATGCTG 
AGCCGCAAGA TATCCGAGCT CCGCCATCGC ACAATACAGC TGCACCGCGA AATTGTCCTG 
ATGAGCAAAT ACAGCAACAC AGAAATTCAT AAAGAGGACC TCCAGCTGGG AATCCCTCCC 
TCCTTCATGA GGTTTCAGCC CCGCCAGCGA GAGGAGATTC TGGAATGGGA GTTTCTGACT 
GGAAAATACT TGTATTCGGC AGTTGACGGC CAGCCCCCTC GAAGAGGAAT GGACTCCGCC 
CAGAGGGAAG CCTTGGACGA CATTGTCATG CAGGTCATGG AGATGATCAA TGCCAACGCC 
AAGACCAGAG GGCGCATCAT TGACTTCAAA GAGATCCAGT ACGGCTACCG CCGGGTGAAC 
CCCATGTATG GGGCTGAGTA CATCCTGGAC CTGCTGCTTC TGTACAAAAA GCACAAAGGG 
AAGAAAATGA CGGTCCCTGT GAGGAGGCAC GCGTATTTAC AGCAGACTTT CAGCAAAATC 
CAGTTTGTGG AGCATGAGGA GCTGGATGCA CAAGAGTTGG CCAAGAGAAT CAATCAGGAA 
TCTGGATCCT TGTCCTTTCT CTCAAACTCC CTGAAGAAGC TCGTCCCCTT TCAGCTCCCT 
GGGTCGAAGA GTGAGCACAA AGAACCCAAA GATAAAAAGA TAAACATACT GATTCCTTTG 
TCTGGGCGTT TCGACATGTT TGTGAGATTT ATGGGAAACT TTGAGAAGAC GTGTCTTATC 
CCCAATCAGA ACGTCAAGCT CGTGGTTCTG CTTTTCAATT CTGACTCCAA CCCTGACAAG 
GCCAAACAAG TTGAACTGAT GAGAGATTAC CGCATTAAGT ACCCTAAAGC CGACATGCAG 
ATTTTGCCTG TGTCTGGAGA GTTTTCAAGA GCCCTGGCCC TGGAAGTAGG ATCCTCCCAG 
TTTAACAATG AATCTTTGCT CTTCTTCTGC GACGTCGACC TCGTGTTTAC TACAGAATTC 
CTTCAGCGAT GTCGAGCAAA TACAGTTCTG GGCCAACAAA TATATTTTCC AATCATCTTC 
AGCCAGTATG ACCCAAAGAT TGTTTATAGT GGGAAAGTTC CCAGTGACAA CCATTTTGCC 
TTTACTCAGA AAACTGGCTT CTGGAGAAAC TATGGGTTTG GCATCACGTG TATTTATAAG 
GGAGATCTTG TCCGAGTGGG TGGCTTTGAT GTTTCCATCC AAGGCTGGGG GCTGGAGGAT 
GTGGACCTTT TCAACAAGGT TGTCCAGGCA GGTTTGAAGA CGTTTAGGAG CCAGGAAGTA 
GGAGTAGTCC ACGTCCACCA TCCTGTCTTT TGTGATCCCA ATCTTGACCC CAAACAGTAC 
AAAATGTGCT TGGGGTCCAA AGCATCGACC TATGGGTCCA CCCAGCAGCT GGCTGAGATG 
TGGCTGGAAA AAAATGATCC AAGTTACAGT AAAAGCAGCA ATAATAATGG CTCAGTGAGG 
ACAGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CHSY1 ( NM_014918.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_055733.2 Homo sapiens chondroitin sulfate synthase 1 (CHSY1), mRNA.

Price & Availability↑

CloneID	OHu22488
Clone ID Related Accession (Same CDS sequence)	NM_014918.5 , NM_014918.4
Accession Version	NM_014918.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2409bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-02
Organism	Homo sapiens(human)
Product	chondroitin sulfate synthase 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AB023207.1, BC046247.1, AC019254.12 and BQ007796.1. This sequence is a reference standard in the RefSeqGene project. On Sep 17, 2009 this sequence version replaced NM_014918.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB023207.1, BC046247.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1966682, SAMEA1968540 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCGCGC GCGGCCGGCG CGCCTGGCTC AGCGTGCTGC TCGGGCTCGT CCTGGGCTTC 
GTGCTGGCCT CGCGGCTCGT CCTGCCCCGG GCTTCCGAGC TGAAGCGAGC GGGCCCACGG 
CGCCGCGCCA GCCCCGAGGG CTGCCGGTCC GGGCAGGCGG CGGCTTCCCA GGCCGGCGGG 
GCGCGCGGCG ATGCGCGCGG GGCGCAGCTC TGGCCGCCCG GCTCGGACCC AGATGGCGGC 
CCGCGCGACA GGAACTTTCT CTTCGTGGGA GTCATGACCG CCCAGAAATA CCTGCAGACT 
CGGGCCGTGG CCGCCTACAG AACATGGTCC AAGACAATTC CTGGGAAAGT TCAGTTCTTC 
TCAAGTGAGG GTTCTGACAC ATCTGTACCA ATTCCAGTAG TGCCACTACG GGGTGTGGAC 
GACTCCTACC CGCCCCAGAA GAAGTCCTTC ATGATGCTCA AGTACATGCA CGACCACTAC 
TTGGACAAGT ATGAATGGTT TATGAGAGCA GATGATGACG TGTACATCAA AGGAGACCGT 
CTGGAGAACT TCCTGAGGAG TTTGAACAGC AGCGAGCCCC TCTTTCTTGG GCAGACAGGC 
CTGGGCACCA CGGAAGAAAT GGGAAAACTG GCCCTGGAGC CTGGTGAGAA CTTCTGCATG 
GGGGGGCCTG GCGTGATCAT GAGCCGGGAG GTGCTTCGGA GAATGGTGCC GCACATTGGC 
AAGTGTCTCC GGGAGATGTA CACCACCCAT GAGGACGTGG AGGTGGGAAG GTGTGTCCGG 
AGGTTTGCAG GGGTGCAGTG TGTCTGGTCT TATGAGATGC AGCAGCTTTT TTATGAGAAT 
TACGAGCAGA ACAAAAAGGG GTACATTAGA GATCTCCATA ACAGTAAAAT TCACCAAGCT 
ATCACATTAC ACCCCAACAA AAACCCACCC TACCAGTACA GGCTCCACAG CTACATGCTG 
AGCCGCAAGA TATCCGAGCT CCGCCATCGC ACAATACAGC TGCACCGCGA AATTGTCCTG 
ATGAGCAAAT ACAGCAACAC AGAAATTCAT AAAGAGGACC TCCAGCTGGG AATCCCTCCC 
TCCTTCATGA GGTTTCAGCC CCGCCAGCGA GAGGAGATTC TGGAATGGGA GTTTCTGACT 
GGAAAATACT TGTATTCGGC AGTTGACGGC CAGCCCCCTC GAAGAGGAAT GGACTCCGCC 
CAGAGGGAAG CCTTGGACGA CATTGTCATG CAGGTCATGG AGATGATCAA TGCCAACGCC 
AAGACCAGAG GGCGCATCAT TGACTTCAAA GAGATCCAGT ACGGCTACCG CCGGGTGAAC 
CCCATGTATG GGGCTGAGTA CATCCTGGAC CTGCTGCTTC TGTACAAAAA GCACAAAGGG 
AAGAAAATGA CGGTCCCTGT GAGGAGGCAC GCGTATTTAC AGCAGACTTT CAGCAAAATC 
CAGTTTGTGG AGCATGAGGA GCTGGATGCA CAAGAGTTGG CCAAGAGAAT CAATCAGGAA 
TCTGGATCCT TGTCCTTTCT CTCAAACTCC CTGAAGAAGC TCGTCCCCTT TCAGCTCCCT 
GGGTCGAAGA GTGAGCACAA AGAACCCAAA GATAAAAAGA TAAACATACT GATTCCTTTG 
TCTGGGCGTT TCGACATGTT TGTGAGATTT ATGGGAAACT TTGAGAAGAC GTGTCTTATC 
CCCAATCAGA ACGTCAAGCT CGTGGTTCTG CTTTTCAATT CTGACTCCAA CCCTGACAAG 
GCCAAACAAG TTGAACTGAT GAGAGATTAC CGCATTAAGT ACCCTAAAGC CGACATGCAG 
ATTTTGCCTG TGTCTGGAGA GTTTTCAAGA GCCCTGGCCC TGGAAGTAGG ATCCTCCCAG 
TTTAACAATG AATCTTTGCT CTTCTTCTGC GACGTCGACC TCGTGTTTAC TACAGAATTC 
CTTCAGCGAT GTCGAGCAAA TACAGTTCTG GGCCAACAAA TATATTTTCC AATCATCTTC 
AGCCAGTATG ACCCAAAGAT TGTTTATAGT GGGAAAGTTC CCAGTGACAA CCATTTTGCC 
TTTACTCAGA AAACTGGCTT CTGGAGAAAC TATGGGTTTG GCATCACGTG TATTTATAAG 
GGAGATCTTG TCCGAGTGGG TGGCTTTGAT GTTTCCATCC AAGGCTGGGG GCTGGAGGAT 
GTGGACCTTT TCAACAAGGT TGTCCAGGCA GGTTTGAAGA CGTTTAGGAG CCAGGAAGTA 
GGAGTAGTCC ACGTCCACCA TCCTGTCTTT TGTGATCCCA ATCTTGACCC CAAACAGTAC 
AAAATGTGCT TGGGGTCCAA AGCATCGACC TATGGGTCCA CCCAGCAGCT GGCTGAGATG 
TGGCTGGAAA AAAATGATCC AAGTTACAGT AAAAGCAGCA ATAATAATGG CTCAGTGAGG 
ACAGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_055733.2
CDS	477..2885
Translation	MAARGRRAWLSVLLGLVLGFVLASRLVLPRASELKRAGPRRRASPEGCRSGQAAASQAGGARGDARGAQLWPPGSDPDGGPRDRNFLFVGVMTAQKYLQTRAVAAYRTWSKTIPGKVQFFSSEGSDTSVPIPVVPLRGVDDSYPPQKKSFMMLKYMHDHYLDKYEWFMRADDDVYIKGDRLENFLRSLNSSEPLFLGQTGLGTTEEMGKLALEPGENFCMGGPGVIMSREVLRRMVPHIGKCLREMYTTHEDVEVGRCVRRFAGVQCVWSYEMQQLFYENYEQNKKGYIRDLHNSKIHQAITLHPNKNPPYQYRLHSYMLSRKISELRHRTIQLHREIVLMSKYSNTEIHKEDLQLGIPPSFMRFQPRQREEILEWEFLTGKYLYSAVDGQPPRRGMDSAQREALDDIVMQVMEMINANAKTRGRIIDFKEIQYGYRRVNPMYGAEYILDLLLLYKKHKGKKMTVPVRRHAYLQQTFSKIQFVEHEELDAQELAKRINQESGSLSFLSNSLKKLVPFQLPGSKSEHKEPKDKKINILIPLSGRFDMFVRFMGNFEKTCLIPNQNVKLVVLLFNSDSNPDKAKQVELMRDYRIKYPKADMQILPVSGEFSRALALEVGSSQFNNESLLFFCDVDLVFTTEFLQRCRANTVLGQQIYFPIIFSQYDPKIVYSGKVPSDNHFAFTQKTGFWRNYGFGITCIYKGDLVRVGGFDVSIQGWGLEDVDLFNKVVQAGLKTFRSQEVGVVHVHHPVFCDPNLDPKQYKMCLGSKASTYGSTQQLAEMWLEKNDPSYSKSSNNNGSVRTA

Target ORF information:

RefSeq Version	NM_014918.4
Organism	Homo sapiens(human)
Definition	Homo sapiens chondroitin sulfate synthase 1 (CHSY1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_014918.4

ORF Insert Sequence:

ATGGCCGCGC GCGGCCGGCG CGCCTGGCTC AGCGTGCTGC TCGGGCTCGT CCTGGGCTTC 
GTGCTGGCCT CGCGGCTCGT CCTGCCCCGG GCTTCCGAGC TGAAGCGAGC GGGCCCACGG 
CGCCGCGCCA GCCCCGAGGG CTGCCGGTCC GGGCAGGCGG CGGCTTCCCA GGCCGGCGGG 
GCGCGCGGCG ATGCGCGCGG GGCGCAGCTC TGGCCGCCCG GCTCGGACCC AGATGGCGGC 
CCGCGCGACA GGAACTTTCT CTTCGTGGGA GTCATGACCG CCCAGAAATA CCTGCAGACT 
CGGGCCGTGG CCGCCTACAG AACATGGTCC AAGACAATTC CTGGGAAAGT TCAGTTCTTC 
TCAAGTGAGG GTTCTGACAC ATCTGTACCA ATTCCAGTAG TGCCACTACG GGGTGTGGAC 
GACTCCTACC CGCCCCAGAA GAAGTCCTTC ATGATGCTCA AGTACATGCA CGACCACTAC 
TTGGACAAGT ATGAATGGTT TATGAGAGCA GATGATGACG TGTACATCAA AGGAGACCGT 
CTGGAGAACT TCCTGAGGAG TTTGAACAGC AGCGAGCCCC TCTTTCTTGG GCAGACAGGC 
CTGGGCACCA CGGAAGAAAT GGGAAAACTG GCCCTGGAGC CTGGTGAGAA CTTCTGCATG 
GGGGGGCCTG GCGTGATCAT GAGCCGGGAG GTGCTTCGGA GAATGGTGCC GCACATTGGC 
AAGTGTCTCC GGGAGATGTA CACCACCCAT GAGGACGTGG AGGTGGGAAG GTGTGTCCGG 
AGGTTTGCAG GGGTGCAGTG TGTCTGGTCT TATGAGATGC AGCAGCTTTT TTATGAGAAT 
TACGAGCAGA ACAAAAAGGG GTACATTAGA GATCTCCATA ACAGTAAAAT TCACCAAGCT 
ATCACATTAC ACCCCAACAA AAACCCACCC TACCAGTACA GGCTCCACAG CTACATGCTG 
AGCCGCAAGA TATCCGAGCT CCGCCATCGC ACAATACAGC TGCACCGCGA AATTGTCCTG 
ATGAGCAAAT ACAGCAACAC AGAAATTCAT AAAGAGGACC TCCAGCTGGG AATCCCTCCC 
TCCTTCATGA GGTTTCAGCC CCGCCAGCGA GAGGAGATTC TGGAATGGGA GTTTCTGACT 
GGAAAATACT TGTATTCGGC AGTTGACGGC CAGCCCCCTC GAAGAGGAAT GGACTCCGCC 
CAGAGGGAAG CCTTGGACGA CATTGTCATG CAGGTCATGG AGATGATCAA TGCCAACGCC 
AAGACCAGAG GGCGCATCAT TGACTTCAAA GAGATCCAGT ACGGCTACCG CCGGGTGAAC 
CCCATGTATG GGGCTGAGTA CATCCTGGAC CTGCTGCTTC TGTACAAAAA GCACAAAGGG 
AAGAAAATGA CGGTCCCTGT GAGGAGGCAC GCGTATTTAC AGCAGACTTT CAGCAAAATC 
CAGTTTGTGG AGCATGAGGA GCTGGATGCA CAAGAGTTGG CCAAGAGAAT CAATCAGGAA 
TCTGGATCCT TGTCCTTTCT CTCAAACTCC CTGAAGAAGC TCGTCCCCTT TCAGCTCCCT 
GGGTCGAAGA GTGAGCACAA AGAACCCAAA GATAAAAAGA TAAACATACT GATTCCTTTG 
TCTGGGCGTT TCGACATGTT TGTGAGATTT ATGGGAAACT TTGAGAAGAC GTGTCTTATC 
CCCAATCAGA ACGTCAAGCT CGTGGTTCTG CTTTTCAATT CTGACTCCAA CCCTGACAAG 
GCCAAACAAG TTGAACTGAT GAGAGATTAC CGCATTAAGT ACCCTAAAGC CGACATGCAG 
ATTTTGCCTG TGTCTGGAGA GTTTTCAAGA GCCCTGGCCC TGGAAGTAGG ATCCTCCCAG 
TTTAACAATG AATCTTTGCT CTTCTTCTGC GACGTCGACC TCGTGTTTAC TACAGAATTC 
CTTCAGCGAT GTCGAGCAAA TACAGTTCTG GGCCAACAAA TATATTTTCC AATCATCTTC 
AGCCAGTATG ACCCAAAGAT TGTTTATAGT GGGAAAGTTC CCAGTGACAA CCATTTTGCC 
TTTACTCAGA AAACTGGCTT CTGGAGAAAC TATGGGTTTG GCATCACGTG TATTTATAAG 
GGAGATCTTG TCCGAGTGGG TGGCTTTGAT GTTTCCATCC AAGGCTGGGG GCTGGAGGAT 
GTGGACCTTT TCAACAAGGT TGTCCAGGCA GGTTTGAAGA CGTTTAGGAG CCAGGAAGTA 
GGAGTAGTCC ACGTCCACCA TCCTGTCTTT TGTGATCCCA ATCTTGACCC CAAACAGTAC 
AAAATGTGCT TGGGGTCCAA AGCATCGACC TATGGGTCCA CCCAGCAGCT GGCTGAGATG 
TGGCTGGAAA AAAATGATCC AAGTTACAGT AAAAGCAGCA ATAATAATGG CTCAGTGAGG 
ACAGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CHSY1 ( XM_011521364.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011519666.1 Homo sapiens chondroitin sulfate synthase 1 (CHSY1), transcript variant X1, mRNA.

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CloneID	OHu64998
Clone ID Related Accession (Same CDS sequence)	XM_011521364.2
Accession Version	XM_011521364.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2493bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	chondroitin sulfate synthase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011521364.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGCGC GCGGCCGGCG CGCCTGGCTC AGCGTGCTGC TCGGGCTCGT CCTGGGCTTC 
GTGCTGGCCT CGCGGCTCGT CCTGCCCCGG GCTTCCGAGC TGAAGCGAGC GGGCCCACGG 
CGCCGCGCCA GCCCCGAGGG CTGCCGGTCC GGGCAGGCGG CGGCTTCCCA GGCCGGCGGG 
GCGCGCGGCG ATGCGCGCGG GGCGCAGCTC TGGCCGCCCG GCTCGGACCC AGATGGCGGC 
CCGCGCGACA GGAACTTTCT CTTCGTGGGA GTCATGACCG CCCAGAAATA CCTGCAGACT 
CGGGCCGTGG CCGCCTACAG AACATGGTCC AAGACAATTC CTGGGAAAGT TCAGTTCTTC 
TCAAGTGAGG GTTCTGACAC ATCTGTACCA ATTCCAGTAG TGCCACTACG GGGTGTGGAC 
GACTCCTACC CGCCCCAGAA GAAGTCCTTC ATGATGCTCA AGTACATGCA CGACCACTAC 
TTGGACAAGT ATGAATGGTT TATGAGAGCA GATGATGACG TGTACATCAA AGGAGACCGT 
CTGGAGAACT TCCTGAGGAG TTTGAACAGC AGCGAGCCCC TCTTTCTTGG GCAGACAGGC 
CTGGGCACCA CGGAAGAAAT GGGAAAACTG GCCCTGGAGC CTGGTGAGAA CTTCTGCATG 
GGGGGGCCTG GCGTGATCAT GAGCCGGGAG GTGCTTCGGA GAATGGTGCC GCACATTGGC 
AAGTGTCTCC GGGAGATGTA CACCACCCAT GAGGACGTGG AGGTGGGAAG GTGTGTCCGG 
AGGTTTGCAG GGGTGCAGTG TGTCTGGTCT TATGAGGCTT CTCCTGTCGC CTGTCTGTGC 
CAGTGTCTTA GGCATTCCTT GTTTTTGATG ATCTTGACGG TTTTGAGGAG TGCTAGTCAG 
ATGCAGCAGC TTTTTTATGA GAATTACGAG CAGAACAAAA AGGGGTACAT TAGAGATCTC 
CATAACAGTA AAATTCACCA AGCTATCACA TTACACCCCA ACAAAAACCC ACCCTACCAG 
TACAGGCTCC ACAGCTACAT GCTGAGCCGC AAGATATCCG AGCTCCGCCA TCGCACAATA 
CAGCTGCACC GCGAAATTGT CCTGATGAGC AAATACAGCA ACACAGAAAT TCATAAAGAG 
GACCTCCAGC TGGGAATCCC TCCCTCCTTC ATGAGGTTTC AGCCCCGCCA GCGAGAGGAG 
ATTCTGGAAT GGGAGTTTCT GACTGGAAAA TACTTGTATT CGGCAGTTGA CGGCCAGCCC 
CCTCGAAGAG GAATGGACTC CGCCCAGAGG GAAGCCTTGG ACGACATTGT CATGCAGGTC 
ATGGAGATGA TCAATGCCAA CGCCAAGACC AGAGGGCGCA TCATTGACTT CAAAGAGATC 
CAGTACGGCT ACCGCCGGGT GAACCCCATG TATGGGGCTG AGTACATCCT GGACCTGCTG 
CTTCTGTACA AAAAGCACAA AGGGAAGAAA ATGACGGTCC CTGTGAGGAG GCACGCGTAT 
TTACAGCAGA CTTTCAGCAA AATCCAGTTT GTGGAGCATG AGGAGCTGGA TGCACAAGAG 
TTGGCCAAGA GAATCAATCA GGAATCTGGA TCCTTGTCCT TTCTCTCAAA CTCCCTGAAG 
AAGCTCGTCC CCTTTCAGCT CCCTGGGTCG AAGAGTGAGC ACAAAGAACC CAAAGATAAA 
AAGATAAACA TACTGATTCC TTTGTCTGGG CGTTTCGACA TGTTTGTGAG ATTTATGGGA 
AACTTTGAGA AGACGTGTCT TATCCCCAAT CAGAACGTCA AGCTCGTGGT TCTGCTTTTC 
AATTCTGACT CCAACCCTGA CAAGGCCAAA CAAGTTGAAC TGATGAGAGA TTACCGCATT 
AAGTACCCTA AAGCCGACAT GCAGATTTTG CCTGTGTCTG GAGAGTTTTC AAGAGCCCTG 
GCCCTGGAAG TAGGATCCTC CCAGTTTAAC AATGAATCTT TGCTCTTCTT CTGCGACGTC 
GACCTCGTGT TTACTACAGA ATTCCTTCAG CGATGTCGAG CAAATACAGT TCTGGGCCAA 
CAAATATATT TTCCAATCAT CTTCAGCCAG TATGACCCAA AGATTGTTTA TAGTGGGAAA 
GTTCCCAGTG ACAACCATTT TGCCTTTACT CAGAAAACTG GCTTCTGGAG AAACTATGGG 
TTTGGCATCA CGTGTATTTA TAAGGGAGAT CTTGTCCGAG TGGGTGGCTT TGATGTTTCC 
ATCCAAGGCT GGGGGCTGGA GGATGTGGAC CTTTTCAACA AGGTTGTCCA GGCAGGTTTG 
AAGACGTTTA GGAGCCAGGA AGTAGGAGTA GTCCACGTCC ACCATCCTGT CTTTTGTGAT 
CCCAATCTTG ACCCCAAACA GTACAAAATG TGCTTGGGGT CCAAAGCATC GACCTATGGG 
TCCACCCAGC AGCTGGCTGA GATGTGGCTG GAAAAAAATG ATCCAAGTTA CAGTAAAAGC 
AGCAATAATA ATGGCTCAGT GAGGACAGCC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011519666.1
CDS	16..2508
Translation	MAARGRRAWLSVLLGLVLGFVLASRLVLPRASELKRAGPRRRASPEGCRSGQAAASQAGGARGDARGAQLWPPGSDPDGGPRDRNFLFVGVMTAQKYLQTRAVAAYRTWSKTIPGKVQFFSSEGSDTSVPIPVVPLRGVDDSYPPQKKSFMMLKYMHDHYLDKYEWFMRADDDVYIKGDRLENFLRSLNSSEPLFLGQTGLGTTEEMGKLALEPGENFCMGGPGVIMSREVLRRMVPHIGKCLREMYTTHEDVEVGRCVRRFAGVQCVWSYEASPVACLCQCLRHSLFLMILTVLRSASQMQQLFYENYEQNKKGYIRDLHNSKIHQAITLHPNKNPPYQYRLHSYMLSRKISELRHRTIQLHREIVLMSKYSNTEIHKEDLQLGIPPSFMRFQPRQREEILEWEFLTGKYLYSAVDGQPPRRGMDSAQREALDDIVMQVMEMINANAKTRGRIIDFKEIQYGYRRVNPMYGAEYILDLLLLYKKHKGKKMTVPVRRHAYLQQTFSKIQFVEHEELDAQELAKRINQESGSLSFLSNSLKKLVPFQLPGSKSEHKEPKDKKINILIPLSGRFDMFVRFMGNFEKTCLIPNQNVKLVVLLFNSDSNPDKAKQVELMRDYRIKYPKADMQILPVSGEFSRALALEVGSSQFNNESLLFFCDVDLVFTTEFLQRCRANTVLGQQIYFPIIFSQYDPKIVYSGKVPSDNHFAFTQKTGFWRNYGFGITCIYKGDLVRVGGFDVSIQGWGLEDVDLFNKVVQAGLKTFRSQEVGVVHVHHPVFCDPNLDPKQYKMCLGSKASTYGSTQQLAEMWLEKNDPSYSKSSNNNGSVRTA

Target ORF information:

RefSeq Version	XM_011521364.2
Organism	Homo sapiens(human)
Definition	Homo sapiens chondroitin sulfate synthase 1 (CHSY1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011521364.2

ORF Insert Sequence:

ATGGCCGCGC GCGGCCGGCG CGCCTGGCTC AGCGTGCTGC TCGGGCTCGT CCTGGGCTTC 
GTGCTGGCCT CGCGGCTCGT CCTGCCCCGG GCTTCCGAGC TGAAGCGAGC GGGCCCACGG 
CGCCGCGCCA GCCCCGAGGG CTGCCGGTCC GGGCAGGCGG CGGCTTCCCA GGCCGGCGGG 
GCGCGCGGCG ATGCGCGCGG GGCGCAGCTC TGGCCGCCCG GCTCGGACCC AGATGGCGGC 
CCGCGCGACA GGAACTTTCT CTTCGTGGGA GTCATGACCG CCCAGAAATA CCTGCAGACT 
CGGGCCGTGG CCGCCTACAG AACATGGTCC AAGACAATTC CTGGGAAAGT TCAGTTCTTC 
TCAAGTGAGG GTTCTGACAC ATCTGTACCA ATTCCAGTAG TGCCACTACG GGGTGTGGAC 
GACTCCTACC CGCCCCAGAA GAAGTCCTTC ATGATGCTCA AGTACATGCA CGACCACTAC 
TTGGACAAGT ATGAATGGTT TATGAGAGCA GATGATGACG TGTACATCAA AGGAGACCGT 
CTGGAGAACT TCCTGAGGAG TTTGAACAGC AGCGAGCCCC TCTTTCTTGG GCAGACAGGC 
CTGGGCACCA CGGAAGAAAT GGGAAAACTG GCCCTGGAGC CTGGTGAGAA CTTCTGCATG 
GGGGGGCCTG GCGTGATCAT GAGCCGGGAG GTGCTTCGGA GAATGGTGCC GCACATTGGC 
AAGTGTCTCC GGGAGATGTA CACCACCCAT GAGGACGTGG AGGTGGGAAG GTGTGTCCGG 
AGGTTTGCAG GGGTGCAGTG TGTCTGGTCT TATGAGGCTT CTCCTGTCGC CTGTCTGTGC 
CAGTGTCTTA GGCATTCCTT GTTTTTGATG ATCTTGACGG TTTTGAGGAG TGCTAGTCAG 
ATGCAGCAGC TTTTTTATGA GAATTACGAG CAGAACAAAA AGGGGTACAT TAGAGATCTC 
CATAACAGTA AAATTCACCA AGCTATCACA TTACACCCCA ACAAAAACCC ACCCTACCAG 
TACAGGCTCC ACAGCTACAT GCTGAGCCGC AAGATATCCG AGCTCCGCCA TCGCACAATA 
CAGCTGCACC GCGAAATTGT CCTGATGAGC AAATACAGCA ACACAGAAAT TCATAAAGAG 
GACCTCCAGC TGGGAATCCC TCCCTCCTTC ATGAGGTTTC AGCCCCGCCA GCGAGAGGAG 
ATTCTGGAAT GGGAGTTTCT GACTGGAAAA TACTTGTATT CGGCAGTTGA CGGCCAGCCC 
CCTCGAAGAG GAATGGACTC CGCCCAGAGG GAAGCCTTGG ACGACATTGT CATGCAGGTC 
ATGGAGATGA TCAATGCCAA CGCCAAGACC AGAGGGCGCA TCATTGACTT CAAAGAGATC 
CAGTACGGCT ACCGCCGGGT GAACCCCATG TATGGGGCTG AGTACATCCT GGACCTGCTG 
CTTCTGTACA AAAAGCACAA AGGGAAGAAA ATGACGGTCC CTGTGAGGAG GCACGCGTAT 
TTACAGCAGA CTTTCAGCAA AATCCAGTTT GTGGAGCATG AGGAGCTGGA TGCACAAGAG 
TTGGCCAAGA GAATCAATCA GGAATCTGGA TCCTTGTCCT TTCTCTCAAA CTCCCTGAAG 
AAGCTCGTCC CCTTTCAGCT CCCTGGGTCG AAGAGTGAGC ACAAAGAACC CAAAGATAAA 
AAGATAAACA TACTGATTCC TTTGTCTGGG CGTTTCGACA TGTTTGTGAG ATTTATGGGA 
AACTTTGAGA AGACGTGTCT TATCCCCAAT CAGAACGTCA AGCTCGTGGT TCTGCTTTTC 
AATTCTGACT CCAACCCTGA CAAGGCCAAA CAAGTTGAAC TGATGAGAGA TTACCGCATT 
AAGTACCCTA AAGCCGACAT GCAGATTTTG CCTGTGTCTG GAGAGTTTTC AAGAGCCCTG 
GCCCTGGAAG TAGGATCCTC CCAGTTTAAC AATGAATCTT TGCTCTTCTT CTGCGACGTC 
GACCTCGTGT TTACTACAGA ATTCCTTCAG CGATGTCGAG CAAATACAGT TCTGGGCCAA 
CAAATATATT TTCCAATCAT CTTCAGCCAG TATGACCCAA AGATTGTTTA TAGTGGGAAA 
GTTCCCAGTG ACAACCATTT TGCCTTTACT CAGAAAACTG GCTTCTGGAG AAACTATGGG 
TTTGGCATCA CGTGTATTTA TAAGGGAGAT CTTGTCCGAG TGGGTGGCTT TGATGTTTCC 
ATCCAAGGCT GGGGGCTGGA GGATGTGGAC CTTTTCAACA AGGTTGTCCA GGCAGGTTTG 
AAGACGTTTA GGAGCCAGGA AGTAGGAGTA GTCCACGTCC ACCATCCTGT CTTTTGTGAT 
CCCAATCTTG ACCCCAAACA GTACAAAATG TGCTTGGGGT CCAAAGCATC GACCTATGGG 
TCCACCCAGC AGCTGGCTGA GATGTGGCTG GAAAAAAATG ATCCAAGTTA CAGTAAAAGC 
AGCAATAATA ATGGCTCAGT GAGGACAGCC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

CHSY1 promotes aggressive phenotypes of hepatocellular carcinoma cells via activation of the hedgehog signaling pathway.
Cancer letters403280-288(2017 Sep)
Liu CH,Lan CT,Chou JF,Tseng TJ,Liao WC

Chondroitin sulfate synthase 1 expression is associated with malignant potential of soft tissue sarcomas with myxoid substance.
Human pathology5015-23(2016 Apr)
Momose T,Yoshimura Y,Harumiya S,Isobe K,Kito M,Fukushima M,Kato H,Nakayama J

A novel CHSY1 gene mutation underlies Temtamy preaxial brachydactyly syndrome in a Pakistani family.
European journal of medical genetics57(1)21-4(2014 Jan)
Sher G,Naeem M

Common variants in left/right asymmetry genes and pathways are associated with relative hand skill.
PLoS genetics9(9)e1003751(2013)
Brandler WM,Morris AP,Evans DM,Scerri TS,Kemp JP,Timpson NJ,St Pourcain B,Smith GD,Ring SM,Stein J,Monaco AP,Talcott JB,Fisher SE,Webber C,Paracchini S

Chondroitin synthases I, II, III and chondroitin sulfate glucuronyltransferase expression in colorectal cancer.
Molecular medicine reports4(2)363-8(2011 Mar-Apr)
Kalathas D,Theocharis DA,Bounias D,Kyriakopoulou D,Papageorgakopoulou N,Stavropoulos MS,Vynios DH

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CHSY1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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