DOLK cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	DOLK
Entrez Gene ID	22845
Full Name	dolichol kinase
Synonyms	CDG1M,DK,DK1,SEC59,TMEM15
General protein information	Preferred Names dolichol kinase Names dolichol kinase SEC59 homolog dolichol kinase 1 transmembrane protein 15
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 9 Map Location: 9q34.11
Summary	The protein encoded by this gene catalyzes the CTP-mediated phosphorylation of dolichol, and is involved in the synthesis of Dol-P-Man, which is an essential glycosyl carrier lipid for C- and O-mannosylation, N- and O-linked glycosylation of proteins, and for the biosynthesis of glycosyl phosphatidylinositol anchors in endoplasmic reticulum. Mutations in this gene are associated with dolichol kinase deficiency.[provided by RefSeq, Apr 2010]. COMPLETENESS: complete on the 3' end.
Disorder MIM:	610746
Disorder Html:	Congenital disorder of glycosylation, type Im, 610768 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_014908.4	NP_055723.1	dolichol kinase
NM_014908.3	NP_055723.1	dolichol kinase

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	DOLK	NP_001264588.1
Xenopus tropicalis (tropical clawed frog)	dolk	XP_002935551.1
Canis lupus familiaris (dog)	DOLK	XP_537820.2
Rattus norvegicus (Norway rat)	Dolk	NP_001101296.1
	DOLK	XP_001109312.1
Bos taurus (cattle)	DOLK	NP_001030442.2
Pan troglodytes (chimpanzee)	DOLK	XP_001162301.1
Mus musculus (house mouse)	Dolk	NP_808316.1
Gallus gallus (chicken)	LOC100859010	XP_004945920.1
Danio rerio (zebrafish)	dolk	NP_001103954.1
Homo sapiens (human)	DOLK	NP_055723.1

Gene Ontology
Bibliography

Related articles in PubMed

Dolichol kinase deficiency (DOLK-CDG): Two new cases and expansion of phenotype.
Rush ET, Baker CV, Rizzo WB
American journal of medical genetics. Part A173(9)2428-2434(2017 Sep)

Architecture of the human interactome defines protein communities and disease networks.
Huttlin EL, Bruckner RJ, Paulo JA, Cannon JR, Ting L, Baltier K, Colby G, Gebreab F, Gygi MP, Parzen H, Szpyt J, Tam S, Zarraga G, Pontano-Vaites L, Swarup S, White AE, Schweppe DK, Rad R, Erickson BK, Obar RA, Guruharsha KG, Li K, Artavanis-Tsakonas S, Gygi SP, Harper JW
Nature545(7655)505-509(2017 05)

Widespread Expansion of Protein Interaction Capabilities by Alternative Splicing.
Yang X, Coulombe-Huntington J, Kang S, Sheynkman GM, Hao T, Richardson A, Sun S, Yang F, Shen YA, Murray RR, Spirohn K, Begg BE, Duran-Frigola M, MacWilliams A, Pevzner SJ, Zhong Q, Trigg SA, Tam S, Ghamsari L, Sahni N, Yi S, Rodriguez MD, Balcha D, Tan G, Costanzo M, Andrews B, Boone C, Zhou XJ, Salehi-Ashtiani K, Charloteaux B, Chen AA, Calderwood MA, Aloy P, Roth FP, Hill DE, Iakoucheva LM, Xia Y, Vidal M
Cell164(4)805-17(2016 Feb)

Autosomal recessive dilated cardiomyopathy due to DOLK mutations results from abnormal dystroglycan O-mannosylation.
Lefeber DJ, de Brouwer AP, Morava E, Riemersma M, Schuurs-Hoeijmakers JH, Absmanner B, Verrijp K, van den Akker WM, Huijben K, Steenbergen G, van Reeuwijk J, Jozwiak A, Zucker N, Lorber A, Lammens M, Knopf C, van Bokhoven H, Gr?newald S, Lehle L, Kapusta L, Mandel H, Wevers RA
PLoS genetics7(12)e1002427(2011 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These patients represent an earlier and more severe form of DOLK-CDG (CDG-1m) with a striking presentation at birth that expands the known phenotypic spectrum.
Title: Dolichol kinase deficiency (DOLK-CDG): Two new cases and expansion of phenotype.

We thus identified a combined deficiency of protein N-glycosylation and alpha-dystroglycan O-mannosylation in patients with nonsyndromic DCM due to autosomal recessive DOLK mutations.
Title: Autosomal recessive dilated cardiomyopathy due to DOLK mutations results from abnormal dystroglycan O-mannosylation.

Mutation in dolichol kinase is associated with a defect in dolichol phosphate biosynthesis causing a new inherited disorder with death in early infancy
Title: A defect in dolichol phosphate biosynthesis causes a new inherited disorder with death in early infancy.

dolichol kinase is a polytopic endoplasmic reticulum membrane protein with a cytoplasmically oriented CTP-binding site
Title: Human dolichol kinase, a polytopic endoplasmic reticulum membrane protein with a cytoplasmically oriented CTP-binding site.

The following DOLK gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DOLK cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu18726	NM_014908.4 Latest version!	Homo sapiens dolichol kinase (DOLK), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
View Old Accession Versions >>
OHu18726	NM_014908.3	Homo sapiens dolichol kinase (DOLK), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DOLK ( NM_014908.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_055723.1 Homo sapiens dolichol kinase (DOLK), mRNA.

Price & Availability↑

CloneID	OHu18726
Clone ID Related Accession (Same CDS sequence)	NM_014908.4 , NM_014908.3
Accession Version	NM_014908.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1617bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-04
Organism	Homo sapiens(human)
Product	dolichol kinase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BF056649.1 and BC035556.1. On Aug 31, 2019 this sequence version replaced NM_014908.3. ##Evidence-Data-START## Transcript is intronless :: BC035556.1, AY358759.1 [ECO:0000345] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGACCCGAG AGTGCCCATC TCCGGCCCCG GGGCCTGGGG CTCCGCTGAG TGGATCGGTG 
CTGGCAGAGG CGGCAGTAGT GTTTGCAGTG GTGCTGAGCA TCCACGCAAC CGTATGGGAC 
CGATACTCGT GGTGCGCCGT GGCCCTCGCA GTGCAGGCCT TCTACGTCCA ATACAAGTGG 
GACCGGCTGC TACAGCAGGG AAGCGCCGTC TTCCAGTTCC GAATGTCCGC AAACAGTGGC 
CTATTGCCCG CCTCCATGGT CATGCCTTTG CTTGGACTAG TCATGAAGGA GCGGTGCCAG 
ACTGCTGGGA ACCCGTTCTT TGAGCGTTTT GGCATTGTGG TGGCAGCCAC TGGCATGGCA 
GTGGCCCTCT TCTCATCAGT GTTGGCGCTC GGCATCACTC GCCCAGTGCC AACCAACACT 
TGTGTCATCT TGGGCTTGGC TGGAGGTGTT ATCATTTATA TCATGAAGCA CTCGTTGAGC 
GTGGGGGAGG TGATCGAAGT CCTGGAAGTC CTTCTGATCT TCGTTTATCT CAACATGATC 
CTGCTGTACC TGCTGCCCCG CTGCTTCACC CCTGGTGAGG CACTGCTGGT ATTGGGTGGC 
ATTAGCTTTG TCCTCAACCA GCTCATCAAG CGCTCTCTGA CACTGGTGGA AAGTCAGGGG 
GACCCAGTGG ACTTCTTCCT GCTGGTGGTG GTAGTAGGGA TGGTACTCAT GGGCATTTTC 
TTCAGCACTC TGTTTGTCTT CATGGACTCA GGCACCTGGG CCTCCTCCAT CTTCTTCCAC 
CTCATGACCT GTGTGCTGAG CCTTGGTGTG GTCCTACCCT GGCTGCACCG GCTCATCCGC 
AGGAATCCCC TGCTCTGGCT TCTTCAGTTT CTCTTCCAGA CAGACACCCG CATCTACCTC 
CTAGCCTATT GGTCTCTGCT GGCCACCTTG GCCTGCCTGG TGGTGCTGTA CCAGAATGCC 
AAGCGGTCAT CTTCCGAGTC CAAGAAGCAC CAGGCCCCCA CCATCGCCCG AAAGTATTTC 
CACCTCATTG TGGTAGCCAC CTACATCCCA GGTATCATCT TTGACCGGCC ACTGCTCTAT 
GTAGCCGCCA CTGTATGCCT GGCGGTCTTC ATCTTCCTGG AGTATGTGCG CTACTTCCGC 
ATCAAGCCTT TGGGTCACAC TCTACGGAGC TTCCTGTCCC TTTTTCTGGA TGAACGAGAC 
AGTGGACCAC TCATTCTGAC ACACATCTAC CTGCTCCTGG GCATGTCTCT TCCCATCTGG 
CTGATCCCCA GACCCTGCAC ACAGAAGGGT AGCCTGGGAG GAGCCAGGGC CCTCGTCCCC 
TATGCCGGTG TCCTGGCTGT GGGTGTGGGT GATACTGTGG CCTCCATCTT CGGTAGCACC 
ATGGGGGAGA TCCGCTGGCC TGGAACCAAA AAGACTTTTG AGGGGACCAT GACATCTATA 
TTTGCGCAGA TCATTTCTGT AGCTCTGATC TTAATCTTTG ACAGTGGAGT GGACCTAAAC 
TACAGTTATG CTTGGATTTT GGGGTCCATC AGCACTGTGT CCCTCCTGGA AGCATACACT 
ACACAGATAG ACAATCTCCT TCTGCCTCTC TACCTCCTGA TATTGCTGAT GGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_055723.1
CDS	301..1917
Translation	MTRECPSPAPGPGAPLSGSVLAEAAVVFAVVLSIHATVWDRYSWCAVALAVQAFYVQYKWDRLLQQGSAVFQFRMSANSGLLPASMVMPLLGLVMKERCQTAGNPFFERFGIVVAATGMAVALFSSVLALGITRPVPTNTCVILGLAGGVIIYIMKHSLSVGEVIEVLEVLLIFVYLNMILLYLLPRCFTPGEALLVLGGISFVLNQLIKRSLTLVESQGDPVDFFLLVVVVGMVLMGIFFSTLFVFMDSGTWASSIFFHLMTCVLSLGVVLPWLHRLIRRNPLLWLLQFLFQTDTRIYLLAYWSLLATLACLVVLYQNAKRSSSESKKHQAPTIARKYFHLIVVATYIPGIIFDRPLLYVAATVCLAVFIFLEYVRYFRIKPLGHTLRSFLSLFLDERDSGPLILTHIYLLLGMSLPIWLIPRPCTQKGSLGGARALVPYAGVLAVGVGDTVASIFGSTMGEIRWPGTKKTFEGTMTSIFAQIISVALILIFDSGVDLNYSYAWILGSISTVSLLEAYTTQIDNLLLPLYLLILLMA

Target ORF information:

RefSeq Version	NM_014908.4
Organism	Homo sapiens(human)
Definition	Homo sapiens dolichol kinase (DOLK), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_014908.4

ORF Insert Sequence:

ATGACCCGAG AGTGCCCATC TCCGGCCCCG GGGCCTGGGG CTCCGCTGAG TGGATCGGTG 
CTGGCAGAGG CGGCAGTAGT GTTTGCAGTG GTGCTGAGCA TCCACGCAAC CGTATGGGAC 
CGATACTCGT GGTGCGCCGT GGCCCTCGCA GTGCAGGCCT TCTACGTCCA ATACAAGTGG 
GACCGGCTGC TACAGCAGGG AAGCGCCGTC TTCCAGTTCC GAATGTCCGC AAACAGTGGC 
CTATTGCCCG CCTCCATGGT CATGCCTTTG CTTGGACTAG TCATGAAGGA GCGGTGCCAG 
ACTGCTGGGA ACCCGTTCTT TGAGCGTTTT GGCATTGTGG TGGCAGCCAC TGGCATGGCA 
GTGGCCCTCT TCTCATCAGT GTTGGCGCTC GGCATCACTC GCCCAGTGCC AACCAACACT 
TGTGTCATCT TGGGCTTGGC TGGAGGTGTT ATCATTTATA TCATGAAGCA CTCGTTGAGC 
GTGGGGGAGG TGATCGAAGT CCTGGAAGTC CTTCTGATCT TCGTTTATCT CAACATGATC 
CTGCTGTACC TGCTGCCCCG CTGCTTCACC CCTGGTGAGG CACTGCTGGT ATTGGGTGGC 
ATTAGCTTTG TCCTCAACCA GCTCATCAAG CGCTCTCTGA CACTGGTGGA AAGTCAGGGG 
GACCCAGTGG ACTTCTTCCT GCTGGTGGTG GTAGTAGGGA TGGTACTCAT GGGCATTTTC 
TTCAGCACTC TGTTTGTCTT CATGGACTCA GGCACCTGGG CCTCCTCCAT CTTCTTCCAC 
CTCATGACCT GTGTGCTGAG CCTTGGTGTG GTCCTACCCT GGCTGCACCG GCTCATCCGC 
AGGAATCCCC TGCTCTGGCT TCTTCAGTTT CTCTTCCAGA CAGACACCCG CATCTACCTC 
CTAGCCTATT GGTCTCTGCT GGCCACCTTG GCCTGCCTGG TGGTGCTGTA CCAGAATGCC 
AAGCGGTCAT CTTCCGAGTC CAAGAAGCAC CAGGCCCCCA CCATCGCCCG AAAGTATTTC 
CACCTCATTG TGGTAGCCAC CTACATCCCA GGTATCATCT TTGACCGGCC ACTGCTCTAT 
GTAGCCGCCA CTGTATGCCT GGCGGTCTTC ATCTTCCTGG AGTATGTGCG CTACTTCCGC 
ATCAAGCCTT TGGGTCACAC TCTACGGAGC TTCCTGTCCC TTTTTCTGGA TGAACGAGAC 
AGTGGACCAC TCATTCTGAC ACACATCTAC CTGCTCCTGG GCATGTCTCT TCCCATCTGG 
CTGATCCCCA GACCCTGCAC ACAGAAGGGT AGCCTGGGAG GAGCCAGGGC CCTCGTCCCC 
TATGCCGGTG TCCTGGCTGT GGGTGTGGGT GATACTGTGG CCTCCATCTT CGGTAGCACC 
ATGGGGGAGA TCCGCTGGCC TGGAACCAAA AAGACTTTTG AGGGGACCAT GACATCTATA 
TTTGCGCAGA TCATTTCTGT AGCTCTGATC TTAATCTTTG ACAGTGGAGT GGACCTAAAC 
TACAGTTATG CTTGGATTTT GGGGTCCATC AGCACTGTGT CCCTCCTGGA AGCATACACT 
ACACAGATAG ACAATCTCCT TCTGCCTCTC TACCTCCTGA TATTGCTGAT GGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DOLK ( NM_014908.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_055723.1 Homo sapiens dolichol kinase (DOLK), mRNA.

Price & Availability↑

CloneID	OHu18726
Clone ID Related Accession (Same CDS sequence)	NM_014908.4 , NM_014908.3
Accession Version	NM_014908.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1617bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-03
Organism	Homo sapiens(human)
Product	dolichol kinase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BF056649.1 and BC035556.1. This sequence is a reference standard in the RefSeqGene project. On Apr 2, 2004 this sequence version replaced NM_014908.2. ##RefSeq-Attributes-START## RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGACCCGAG AGTGCCCATC TCCGGCCCCG GGGCCTGGGG CTCCGCTGAG TGGATCGGTG 
CTGGCAGAGG CGGCAGTAGT GTTTGCAGTG GTGCTGAGCA TCCACGCAAC CGTATGGGAC 
CGATACTCGT GGTGCGCCGT GGCCCTCGCA GTGCAGGCCT TCTACGTCCA ATACAAGTGG 
GACCGGCTGC TACAGCAGGG AAGCGCCGTC TTCCAGTTCC GAATGTCCGC AAACAGTGGC 
CTATTGCCCG CCTCCATGGT CATGCCTTTG CTTGGACTAG TCATGAAGGA GCGGTGCCAG 
ACTGCTGGGA ACCCGTTCTT TGAGCGTTTT GGCATTGTGG TGGCAGCCAC TGGCATGGCA 
GTGGCCCTCT TCTCATCAGT GTTGGCGCTC GGCATCACTC GCCCAGTGCC AACCAACACT 
TGTGTCATCT TGGGCTTGGC TGGAGGTGTT ATCATTTATA TCATGAAGCA CTCGTTGAGC 
GTGGGGGAGG TGATCGAAGT CCTGGAAGTC CTTCTGATCT TCGTTTATCT CAACATGATC 
CTGCTGTACC TGCTGCCCCG CTGCTTCACC CCTGGTGAGG CACTGCTGGT ATTGGGTGGC 
ATTAGCTTTG TCCTCAACCA GCTCATCAAG CGCTCTCTGA CACTGGTGGA AAGTCAGGGG 
GACCCAGTGG ACTTCTTCCT GCTGGTGGTG GTAGTAGGGA TGGTACTCAT GGGCATTTTC 
TTCAGCACTC TGTTTGTCTT CATGGACTCA GGCACCTGGG CCTCCTCCAT CTTCTTCCAC 
CTCATGACCT GTGTGCTGAG CCTTGGTGTG GTCCTACCCT GGCTGCACCG GCTCATCCGC 
AGGAATCCCC TGCTCTGGCT TCTTCAGTTT CTCTTCCAGA CAGACACCCG CATCTACCTC 
CTAGCCTATT GGTCTCTGCT GGCCACCTTG GCCTGCCTGG TGGTGCTGTA CCAGAATGCC 
AAGCGGTCAT CTTCCGAGTC CAAGAAGCAC CAGGCCCCCA CCATCGCCCG AAAGTATTTC 
CACCTCATTG TGGTAGCCAC CTACATCCCA GGTATCATCT TTGACCGGCC ACTGCTCTAT 
GTAGCCGCCA CTGTATGCCT GGCGGTCTTC ATCTTCCTGG AGTATGTGCG CTACTTCCGC 
ATCAAGCCTT TGGGTCACAC TCTACGGAGC TTCCTGTCCC TTTTTCTGGA TGAACGAGAC 
AGTGGACCAC TCATTCTGAC ACACATCTAC CTGCTCCTGG GCATGTCTCT TCCCATCTGG 
CTGATCCCCA GACCCTGCAC ACAGAAGGGT AGCCTGGGAG GAGCCAGGGC CCTCGTCCCC 
TATGCCGGTG TCCTGGCTGT GGGTGTGGGT GATACTGTGG CCTCCATCTT CGGTAGCACC 
ATGGGGGAGA TCCGCTGGCC TGGAACCAAA AAGACTTTTG AGGGGACCAT GACATCTATA 
TTTGCGCAGA TCATTTCTGT AGCTCTGATC TTAATCTTTG ACAGTGGAGT GGACCTAAAC 
TACAGTTATG CTTGGATTTT GGGGTCCATC AGCACTGTGT CCCTCCTGGA AGCATACACT 
ACACAGATAG ACAATCTCCT TCTGCCTCTC TACCTCCTGA TATTGCTGAT GGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_055723.1
CDS	431..2047
Translation	MTRECPSPAPGPGAPLSGSVLAEAAVVFAVVLSIHATVWDRYSWCAVALAVQAFYVQYKWDRLLQQGSAVFQFRMSANSGLLPASMVMPLLGLVMKERCQTAGNPFFERFGIVVAATGMAVALFSSVLALGITRPVPTNTCVILGLAGGVIIYIMKHSLSVGEVIEVLEVLLIFVYLNMILLYLLPRCFTPGEALLVLGGISFVLNQLIKRSLTLVESQGDPVDFFLLVVVVGMVLMGIFFSTLFVFMDSGTWASSIFFHLMTCVLSLGVVLPWLHRLIRRNPLLWLLQFLFQTDTRIYLLAYWSLLATLACLVVLYQNAKRSSSESKKHQAPTIARKYFHLIVVATYIPGIIFDRPLLYVAATVCLAVFIFLEYVRYFRIKPLGHTLRSFLSLFLDERDSGPLILTHIYLLLGMSLPIWLIPRPCTQKGSLGGARALVPYAGVLAVGVGDTVASIFGSTMGEIRWPGTKKTFEGTMTSIFAQIISVALILIFDSGVDLNYSYAWILGSISTVSLLEAYTTQIDNLLLPLYLLILLMA

Target ORF information:

RefSeq Version	NM_014908.3
Organism	Homo sapiens(human)
Definition	Homo sapiens dolichol kinase (DOLK), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_014908.3

ORF Insert Sequence:

ATGACCCGAG AGTGCCCATC TCCGGCCCCG GGGCCTGGGG CTCCGCTGAG TGGATCGGTG 
CTGGCAGAGG CGGCAGTAGT GTTTGCAGTG GTGCTGAGCA TCCACGCAAC CGTATGGGAC 
CGATACTCGT GGTGCGCCGT GGCCCTCGCA GTGCAGGCCT TCTACGTCCA ATACAAGTGG 
GACCGGCTGC TACAGCAGGG AAGCGCCGTC TTCCAGTTCC GAATGTCCGC AAACAGTGGC 
CTATTGCCCG CCTCCATGGT CATGCCTTTG CTTGGACTAG TCATGAAGGA GCGGTGCCAG 
ACTGCTGGGA ACCCGTTCTT TGAGCGTTTT GGCATTGTGG TGGCAGCCAC TGGCATGGCA 
GTGGCCCTCT TCTCATCAGT GTTGGCGCTC GGCATCACTC GCCCAGTGCC AACCAACACT 
TGTGTCATCT TGGGCTTGGC TGGAGGTGTT ATCATTTATA TCATGAAGCA CTCGTTGAGC 
GTGGGGGAGG TGATCGAAGT CCTGGAAGTC CTTCTGATCT TCGTTTATCT CAACATGATC 
CTGCTGTACC TGCTGCCCCG CTGCTTCACC CCTGGTGAGG CACTGCTGGT ATTGGGTGGC 
ATTAGCTTTG TCCTCAACCA GCTCATCAAG CGCTCTCTGA CACTGGTGGA AAGTCAGGGG 
GACCCAGTGG ACTTCTTCCT GCTGGTGGTG GTAGTAGGGA TGGTACTCAT GGGCATTTTC 
TTCAGCACTC TGTTTGTCTT CATGGACTCA GGCACCTGGG CCTCCTCCAT CTTCTTCCAC 
CTCATGACCT GTGTGCTGAG CCTTGGTGTG GTCCTACCCT GGCTGCACCG GCTCATCCGC 
AGGAATCCCC TGCTCTGGCT TCTTCAGTTT CTCTTCCAGA CAGACACCCG CATCTACCTC 
CTAGCCTATT GGTCTCTGCT GGCCACCTTG GCCTGCCTGG TGGTGCTGTA CCAGAATGCC 
AAGCGGTCAT CTTCCGAGTC CAAGAAGCAC CAGGCCCCCA CCATCGCCCG AAAGTATTTC 
CACCTCATTG TGGTAGCCAC CTACATCCCA GGTATCATCT TTGACCGGCC ACTGCTCTAT 
GTAGCCGCCA CTGTATGCCT GGCGGTCTTC ATCTTCCTGG AGTATGTGCG CTACTTCCGC 
ATCAAGCCTT TGGGTCACAC TCTACGGAGC TTCCTGTCCC TTTTTCTGGA TGAACGAGAC 
AGTGGACCAC TCATTCTGAC ACACATCTAC CTGCTCCTGG GCATGTCTCT TCCCATCTGG 
CTGATCCCCA GACCCTGCAC ACAGAAGGGT AGCCTGGGAG GAGCCAGGGC CCTCGTCCCC 
TATGCCGGTG TCCTGGCTGT GGGTGTGGGT GATACTGTGG CCTCCATCTT CGGTAGCACC 
ATGGGGGAGA TCCGCTGGCC TGGAACCAAA AAGACTTTTG AGGGGACCAT GACATCTATA 
TTTGCGCAGA TCATTTCTGT AGCTCTGATC TTAATCTTTG ACAGTGGAGT GGACCTAAAC 
TACAGTTATG CTTGGATTTT GGGGTCCATC AGCACTGTGT CCCTCCTGGA AGCATACACT 
ACACAGATAG ACAATCTCCT TCTGCCTCTC TACCTCCTGA TATTGCTGAT GGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Dolichol kinase deficiency (DOLK-CDG): Two new cases and expansion of phenotype.
American journal of medical genetics. Part A173(9)2428-2434(2017 Sep)
Rush ET,Baker CV,Rizzo WB

Architecture of the human interactome defines protein communities and disease networks.
Nature545(7655)505-509(2017 05)
Huttlin EL,Bruckner RJ,Paulo JA,Cannon JR,Ting L,Baltier K,Colby G,Gebreab F,Gygi MP,Parzen H,Szpyt J,Tam S,Zarraga G,Pontano-Vaites L,Swarup S,White AE,Schweppe DK,Rad R,Erickson BK,Obar RA,Guruharsha KG,Li K,Artavanis-Tsakonas S,Gygi SP,Harper JW

Widespread Expansion of Protein Interaction Capabilities by Alternative Splicing.
Cell164(4)805-17(2016 Feb)
Yang X,Coulombe-Huntington J,Kang S,Sheynkman GM,Hao T,Richardson A,Sun S,Yang F,Shen YA,Murray RR,Spirohn K,Begg BE,Duran-Frigola M,MacWilliams A,Pevzner SJ,Zhong Q,Trigg SA,Tam S,Ghamsari L,Sahni N,Yi S,Rodriguez MD,Balcha D,Tan G,Costanzo M,Andrews B,Boone C,Zhou XJ,Salehi-Ashtiani K,Charloteaux B,Chen AA,Calderwood MA,Aloy P,Roth FP,Hill DE,Iakoucheva LM,Xia Y,Vidal M

Autosomal recessive dilated cardiomyopathy due to DOLK mutations results from abnormal dystroglycan O-mannosylation.
PLoS genetics7(12)e1002427(2011 Dec)
Lefeber DJ,de Brouwer AP,Morava E,Riemersma M,Schuurs-Hoeijmakers JH,Absmanner B,Verrijp K,van den Akker WM,Huijben K,Steenbergen G,van Reeuwijk J,Jozwiak A,Zucker N,Lorber A,Lammens M,Knopf C,van Bokhoven H,Gr?newald S,Lehle L,Kapusta L,Mandel H,Wevers RA

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DOLK cDNA ORF clone, Homo sapiens(human)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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