PUF60 cDNA ORF clone, Homo sapiens(human)

The following PUF60 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PUF60 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu20077 NM_014281.5
Latest version!
Homo sapiens poly(U) binding splicing factor 60 (PUF60), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$219.50-$307.30
$439.00
OHu22582 NM_001136033.3
Latest version!
Homo sapiens poly(U) binding splicing factor 60 (PUF60), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$219.50-$307.30
$439.00
OHu23020 NM_001271100.2
Latest version!
Homo sapiens poly(U) binding splicing factor 60 (PUF60), transcript variant 8, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$159.50-$223.30
$319.00
OHu22582 XM_017013239.1
Latest version!
Homo sapiens poly(U) binding splicing factor 60 (PUF60), transcript variant X17, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$219.50-$307.30
$439.00
OHu23020 XM_017013240.1
Latest version!
Homo sapiens poly(U) binding splicing factor 60 (PUF60), transcript variant X18, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$159.50-$223.30
$319.00
OHu20314 NM_078480.3
Latest version!
Homo sapiens poly(U) binding splicing factor 60 (PUF60), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $307.30
$439.00
OHu20744 NM_001271096.2
Latest version!
Homo sapiens poly(U) binding splicing factor 60 (PUF60), transcript variant 4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $307.30
$439.00
OHu21541 NM_001271098.2
Latest version!
Homo sapiens poly(U) binding splicing factor 60 (PUF60), transcript variant 6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $307.30
$439.00
OHu27468 NM_001271099.2
Latest version!
Homo sapiens poly(U) binding splicing factor 60 (PUF60), transcript variant 7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $307.30
$439.00
OHu27624 NM_001271097.2
Latest version!
Homo sapiens poly(U) binding splicing factor 60 (PUF60), transcript variant 5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $307.30
$439.00
OHu64963 NM_001362895.2
Latest version!
Homo sapiens poly(U) binding splicing factor 60 (PUF60), transcript variant 9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $307.30
$439.00
OHu64963 NM_001362896.2
Latest version!
Homo sapiens poly(U) binding splicing factor 60 (PUF60), transcript variant 10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $307.30
$439.00
OHu64964 NM_001362897.2
Latest version!
Homo sapiens poly(U) binding splicing factor 60 (PUF60), transcript variant 11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $307.30
$439.00
OHu64963 XM_017013234.1
Latest version!
Homo sapiens poly(U) binding splicing factor 60 (PUF60), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $307.30
$439.00
OHu64963 XM_017013238.1
Latest version!
Homo sapiens poly(U) binding splicing factor 60KDa (PUF60), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $307.30
$439.00
OHu64963 XM_017013237.1
Latest version!
Homo sapiens poly(U) binding splicing factor 60KDa (PUF60), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $307.30
$439.00
OHu64963 XM_011516929.2
Latest version!
Homo sapiens poly(U) binding splicing factor 60KDa (PUF60), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $307.30
$439.00
OHu64964 XM_017013236.1
Latest version!
Homo sapiens poly(U) binding splicing factor 60 (PUF60), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $307.30
$439.00
OHu64964 XM_011516930.2
Latest version!
Homo sapiens poly(U) binding splicing factor 60KDa (PUF60), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $307.30
$439.00
OHu97848 XM_017013235.1
Latest version!
Homo sapiens poly(U) binding splicing factor 60 (PUF60), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $307.30
$439.00
View Old Accession Versions >>
OHu64964 NM_001362897.1 Homo sapiens poly(U) binding splicing factor 60 (PUF60), transcript variant 11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $307.30
$439.00
OHu27624 NM_001271097.1 Homo sapiens poly(U) binding splicing factor 60 (PUF60), transcript variant 5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $307.30
$439.00
OHu23020 NM_001271100.1 Homo sapiens poly(U) binding splicing factor 60 (PUF60), transcript variant 8, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$159.50-$223.30
$319.00
OHu22582 NM_001136033.2 Homo sapiens poly(U) binding splicing factor 60 (PUF60), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$219.50-$307.30
$439.00
OHu20077 NM_014281.4 Homo sapiens poly(U) binding splicing factor 60 (PUF60), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$219.50-$307.30
$439.00
OHu64963 NM_001362896.1 Homo sapiens poly(U) binding splicing factor 60 (PUF60), transcript variant 10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $307.30
$439.00
OHu27468 NM_001271099.1 Homo sapiens poly(U) binding splicing factor 60 (PUF60), transcript variant 7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $307.30
$439.00
OHu20744 NM_001271096.1 Homo sapiens poly(U) binding splicing factor 60 (PUF60), transcript variant 4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $307.30
$439.00
OHu21541 NM_001271098.1 Homo sapiens poly(U) binding splicing factor 60 (PUF60), transcript variant 6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $307.30
$439.00
OHu20314 NM_078480.2 Homo sapiens poly(U) binding splicing factor 60 (PUF60), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $307.30
$439.00
OHu64963 NM_001362895.1 Homo sapiens poly(U) binding splicing factor 60 (PUF60), transcript variant 9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $307.30
$439.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu20077
    Clone ID Related Accession (Same CDS sequence) NM_014281.4 , NM_014281.5
    Accession Version NM_014281.4 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1629bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-10-20
    Organism Homo sapiens(human)
    Product poly(U)-binding-splicing factor PUF60 isoform b
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CN371576.1, AF217197.2 and AW249619.1. On Sep 1, 2012 this sequence version replaced NM_014281.3. Transcript Variant: This variant (2) lacks an alternate in-frame exon in the coding region, compared to variant 1. The encoded isoform (b) is shorter than isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163655.487795.1, BC008875.2 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGGCGACGG CGACCATAGC TCTCCAGGTC AATGGCCAGC AAGGAGGGGG GTCCGAGCCG 
    GCGGCGGCGG CGGCAGTGGT GGCAGCGGGA GACAAATGGA AACCTCCACA GGGCACAGAC
    TCCATCAAGA TGGAGAACGG GCAGAGCACA GCCGCCAAGC TGGGGCTGCC TCCCCTGACG
    CCCGAGCAGC AGGAGGCCCT TCAGAAGGCC AAGAAGTACG CCATGGAGCA GAGCATCAAG
    AGTGTGCTGG TGAAGCAGAC CATCGCGCAC CAGCAGCAGC AGCTCACCAA CCTGCAGATG
    GCGGCTCAGC GGCAGCGGGC GCTGGCCATC ATGTGCCGCG TCTACGTGGG CTCTATCTAC
    TATGAGCTGG GGGAGGACAC CATCCGCCAG GCCTTTGCCC CCTTTGGCCC CATCAAGAGC
    ATCGACATGT CCTGGGACTC CGTCACCATG AAGCACAAGG GCTTTGCCTT CGTGGAGTAT
    GAGGTCCCCG AAGCTGCACA GCTGGCCTTG GAGCAGATGA ACTCGGTGAT GCTGGGGGGC
    AGGAACATCA AGGTGGGCAG ACCCAGCAAC ATAGGGCAGG CCCAGCCCAT CATAGACCAG
    TTGGCTGAGG AGGCACGGGC CTTCAACCGC ATCTACGTGG CCTCTGTGCA CCAGGACCTC
    TCAGACGATG ACATCAAGAG CGTGTTTGAG GCCTTTGGCA AGATCAAGTC CTGCACACTG
    GCCCGGGACC CCACAACTGG CAAGCACAAG GGCTACGGCT TCATTGAGTA CGAGAAGGCC
    CAGTCGTCCC AAGATGCTGT GTCTTCCATG AACCTCTTTG ACCTGGGTGG CCAGTACTTG
    CGGGTGGGCA AGGCTGTCAC ACCGCCCATG CCCCTACTCA CACCAGCCAC GCCTGGAGGC
    CTCCCACCTG CCGCTGCTGT GGCAGCTGCT GCAGCCACTG CCAAGATCAC AGCTCAGGAA
    GCAGTGGCCG GAGCAGCGGT GCTGGGTACC CTGGGCACAC CTGGACTGGT GTCCCCAGCA
    CTGACCCTGG CCCAGCCCCT GGGCACTTTG CCCCAGGCTG TCATGGCTGC CCAGGCACCT
    GGAGTCATCA CAGGTGTGAC CCCAGCCCGT CCTCCTATCC CGGTCACCAT CCCCTCGGTG
    GGAGTGGTGA ACCCCATCCT GGCCAGCCCT CCAACGCTGG GTCTCCTGGA GCCCAAGAAG
    GAGAAGGAAG AAGAGGAGCT GTTTCCCGAG TCAGAGCGGC CAGAGATGCT GAGCGAGCAG
    GAGCACATGA GCATCTCGGG CAGTAGCGCC CGACACATGG TGATGCAGAA GCTGCTCCGC
    AAGCAGGAGT CTACAGTGAT GGTTCTGCGC AACATGGTGG ACCCCAAGGA CATCGATGAT
    GACCTGGAAG GGGAGGTGAC AGAGGAGTGT GGCAAGTTCG GGGCCGTGAA CCGCGTCATC
    ATCTACCAAG AGAAACAAGG CGAGGAGGAG GATGCAGAAA TCATTGTCAA GATCTTTGTG
    GAGTTTTCCA TAGCCTCTGA GACTCATAAG GCCATCCAGG CCCTCAATGG CCGCTGGTTT
    GCTGGCCGCA AGGTGGTGGC TGAAGTGTAC GACCAGGAGC GTTTTGATAA CAGTGACCTC
    TCTGCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_055096.2
    CDS84..1712
    Translation

    Target ORF information:

    RefSeq Version NM_014281.4
    Organism Homo sapiens(human)
    Definition Homo sapiens poly(U) binding splicing factor 60 (PUF60), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_014281.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGGCGACGG CGACCATAGC TCTCCAGGTC AATGGCCAGC AAGGAGGGGG GTCCGAGCCG 
    GCGGCGGCGG CGGCAGTGGT GGCAGCGGGA GACAAATGGA AACCTCCACA GGGCACAGAC
    TCCATCAAGA TGGAGAACGG GCAGAGCACA GCCGCCAAGC TGGGGCTGCC TCCCCTGACG
    CCCGAGCAGC AGGAGGCCCT TCAGAAGGCC AAGAAGTACG CCATGGAGCA GAGCATCAAG
    AGTGTGCTGG TGAAGCAGAC CATCGCGCAC CAGCAGCAGC AGCTCACCAA CCTGCAGATG
    GCGGCTCAGC GGCAGCGGGC GCTGGCCATC ATGTGCCGCG TCTACGTGGG CTCTATCTAC
    TATGAGCTGG GGGAGGACAC CATCCGCCAG GCCTTTGCCC CCTTTGGCCC CATCAAGAGC
    ATCGACATGT CCTGGGACTC CGTCACCATG AAGCACAAGG GCTTTGCCTT CGTGGAGTAT
    GAGGTCCCCG AAGCTGCACA GCTGGCCTTG GAGCAGATGA ACTCGGTGAT GCTGGGGGGC
    AGGAACATCA AGGTGGGCAG ACCCAGCAAC ATAGGGCAGG CCCAGCCCAT CATAGACCAG
    TTGGCTGAGG AGGCACGGGC CTTCAACCGC ATCTACGTGG CCTCTGTGCA CCAGGACCTC
    TCAGACGATG ACATCAAGAG CGTGTTTGAG GCCTTTGGCA AGATCAAGTC CTGCACACTG
    GCCCGGGACC CCACAACTGG CAAGCACAAG GGCTACGGCT TCATTGAGTA CGAGAAGGCC
    CAGTCGTCCC AAGATGCTGT GTCTTCCATG AACCTCTTTG ACCTGGGTGG CCAGTACTTG
    CGGGTGGGCA AGGCTGTCAC ACCGCCCATG CCCCTACTCA CACCAGCCAC GCCTGGAGGC
    CTCCCACCTG CCGCTGCTGT GGCAGCTGCT GCAGCCACTG CCAAGATCAC AGCTCAGGAA
    GCAGTGGCCG GAGCAGCGGT GCTGGGTACC CTGGGCACAC CTGGACTGGT GTCCCCAGCA
    CTGACCCTGG CCCAGCCCCT GGGCACTTTG CCCCAGGCTG TCATGGCTGC CCAGGCACCT
    GGAGTCATCA CAGGTGTGAC CCCAGCCCGT CCTCCTATCC CGGTCACCAT CCCCTCGGTG
    GGAGTGGTGA ACCCCATCCT GGCCAGCCCT CCAACGCTGG GTCTCCTGGA GCCCAAGAAG
    GAGAAGGAAG AAGAGGAGCT GTTTCCCGAG TCAGAGCGGC CAGAGATGCT GAGCGAGCAG
    GAGCACATGA GCATCTCGGG CAGTAGCGCC CGACACATGG TGATGCAGAA GCTGCTCCGC
    AAGCAGGAGT CTACAGTGAT GGTTCTGCGC AACATGGTGG ACCCCAAGGA CATCGATGAT
    GACCTGGAAG GGGAGGTGAC AGAGGAGTGT GGCAAGTTCG GGGCCGTGAA CCGCGTCATC
    ATCTACCAAG AGAAACAAGG CGAGGAGGAG GATGCAGAAA TCATTGTCAA GATCTTTGTG
    GAGTTTTCCA TAGCCTCTGA GACTCATAAG GCCATCCAGG CCCTCAATGG CCGCTGGTTT
    GCTGGCCGCA AGGTGGTGGC TGAAGTGTAC GACCAGGAGC GTTTTGATAA CAGTGACCTC
    TCTGCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu20077
    Clone ID Related Accession (Same CDS sequence) NM_014281.4 , NM_014281.5
    Accession Version NM_014281.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1629bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-27
    Organism Homo sapiens(human)
    Product poly(U)-binding-splicing factor PUF60 isoform b
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CN371576.1, AF217197.2 and AW249619.1. On May 31, 2019 this sequence version replaced NM_014281.4. Transcript Variant: This variant (2) lacks an alternate in-frame exon in the coding region, compared to variant 1. The encoded isoform (b) is shorter than isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.86649.1, SRR1803615.37601.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGGCGACGG CGACCATAGC TCTCCAGGTC AATGGCCAGC AAGGAGGGGG GTCCGAGCCG 
    GCGGCGGCGG CGGCAGTGGT GGCAGCGGGA GACAAATGGA AACCTCCACA GGGCACAGAC
    TCCATCAAGA TGGAGAACGG GCAGAGCACA GCCGCCAAGC TGGGGCTGCC TCCCCTGACG
    CCCGAGCAGC AGGAGGCCCT TCAGAAGGCC AAGAAGTACG CCATGGAGCA GAGCATCAAG
    AGTGTGCTGG TGAAGCAGAC CATCGCGCAC CAGCAGCAGC AGCTCACCAA CCTGCAGATG
    GCGGCTCAGC GGCAGCGGGC GCTGGCCATC ATGTGCCGCG TCTACGTGGG CTCTATCTAC
    TATGAGCTGG GGGAGGACAC CATCCGCCAG GCCTTTGCCC CCTTTGGCCC CATCAAGAGC
    ATCGACATGT CCTGGGACTC CGTCACCATG AAGCACAAGG GCTTTGCCTT CGTGGAGTAT
    GAGGTCCCCG AAGCTGCACA GCTGGCCTTG GAGCAGATGA ACTCGGTGAT GCTGGGGGGC
    AGGAACATCA AGGTGGGCAG ACCCAGCAAC ATAGGGCAGG CCCAGCCCAT CATAGACCAG
    TTGGCTGAGG AGGCACGGGC CTTCAACCGC ATCTACGTGG CCTCTGTGCA CCAGGACCTC
    TCAGACGATG ACATCAAGAG CGTGTTTGAG GCCTTTGGCA AGATCAAGTC CTGCACACTG
    GCCCGGGACC CCACAACTGG CAAGCACAAG GGCTACGGCT TCATTGAGTA CGAGAAGGCC
    CAGTCGTCCC AAGATGCTGT GTCTTCCATG AACCTCTTTG ACCTGGGTGG CCAGTACTTG
    CGGGTGGGCA AGGCTGTCAC ACCGCCCATG CCCCTACTCA CACCAGCCAC GCCTGGAGGC
    CTCCCACCTG CCGCTGCTGT GGCAGCTGCT GCAGCCACTG CCAAGATCAC AGCTCAGGAA
    GCAGTGGCCG GAGCAGCGGT GCTGGGTACC CTGGGCACAC CTGGACTGGT GTCCCCAGCA
    CTGACCCTGG CCCAGCCCCT GGGCACTTTG CCCCAGGCTG TCATGGCTGC CCAGGCACCT
    GGAGTCATCA CAGGTGTGAC CCCAGCCCGT CCTCCTATCC CGGTCACCAT CCCCTCGGTG
    GGAGTGGTGA ACCCCATCCT GGCCAGCCCT CCAACGCTGG GTCTCCTGGA GCCCAAGAAG
    GAGAAGGAAG AAGAGGAGCT GTTTCCCGAG TCAGAGCGGC CAGAGATGCT GAGCGAGCAG
    GAGCACATGA GCATCTCGGG CAGTAGCGCC CGACACATGG TGATGCAGAA GCTGCTCCGC
    AAGCAGGAGT CTACAGTGAT GGTTCTGCGC AACATGGTGG ACCCCAAGGA CATCGATGAT
    GACCTGGAAG GGGAGGTGAC AGAGGAGTGT GGCAAGTTCG GGGCCGTGAA CCGCGTCATC
    ATCTACCAAG AGAAACAAGG CGAGGAGGAG GATGCAGAAA TCATTGTCAA GATCTTTGTG
    GAGTTTTCCA TAGCCTCTGA GACTCATAAG GCCATCCAGG CCCTCAATGG CCGCTGGTTT
    GCTGGCCGCA AGGTGGTGGC TGAAGTGTAC GACCAGGAGC GTTTTGATAA CAGTGACCTC
    TCTGCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_055096.2
    CDS13..1641
    Translation

    Target ORF information:

    RefSeq Version NM_014281.5
    Organism Homo sapiens(human)
    Definition Homo sapiens poly(U) binding splicing factor 60 (PUF60), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_014281.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGGCGACGG CGACCATAGC TCTCCAGGTC AATGGCCAGC AAGGAGGGGG GTCCGAGCCG 
    GCGGCGGCGG CGGCAGTGGT GGCAGCGGGA GACAAATGGA AACCTCCACA GGGCACAGAC
    TCCATCAAGA TGGAGAACGG GCAGAGCACA GCCGCCAAGC TGGGGCTGCC TCCCCTGACG
    CCCGAGCAGC AGGAGGCCCT TCAGAAGGCC AAGAAGTACG CCATGGAGCA GAGCATCAAG
    AGTGTGCTGG TGAAGCAGAC CATCGCGCAC CAGCAGCAGC AGCTCACCAA CCTGCAGATG
    GCGGCTCAGC GGCAGCGGGC GCTGGCCATC ATGTGCCGCG TCTACGTGGG CTCTATCTAC
    TATGAGCTGG GGGAGGACAC CATCCGCCAG GCCTTTGCCC CCTTTGGCCC CATCAAGAGC
    ATCGACATGT CCTGGGACTC CGTCACCATG AAGCACAAGG GCTTTGCCTT CGTGGAGTAT
    GAGGTCCCCG AAGCTGCACA GCTGGCCTTG GAGCAGATGA ACTCGGTGAT GCTGGGGGGC
    AGGAACATCA AGGTGGGCAG ACCCAGCAAC ATAGGGCAGG CCCAGCCCAT CATAGACCAG
    TTGGCTGAGG AGGCACGGGC CTTCAACCGC ATCTACGTGG CCTCTGTGCA CCAGGACCTC
    TCAGACGATG ACATCAAGAG CGTGTTTGAG GCCTTTGGCA AGATCAAGTC CTGCACACTG
    GCCCGGGACC CCACAACTGG CAAGCACAAG GGCTACGGCT TCATTGAGTA CGAGAAGGCC
    CAGTCGTCCC AAGATGCTGT GTCTTCCATG AACCTCTTTG ACCTGGGTGG CCAGTACTTG
    CGGGTGGGCA AGGCTGTCAC ACCGCCCATG CCCCTACTCA CACCAGCCAC GCCTGGAGGC
    CTCCCACCTG CCGCTGCTGT GGCAGCTGCT GCAGCCACTG CCAAGATCAC AGCTCAGGAA
    GCAGTGGCCG GAGCAGCGGT GCTGGGTACC CTGGGCACAC CTGGACTGGT GTCCCCAGCA
    CTGACCCTGG CCCAGCCCCT GGGCACTTTG CCCCAGGCTG TCATGGCTGC CCAGGCACCT
    GGAGTCATCA CAGGTGTGAC CCCAGCCCGT CCTCCTATCC CGGTCACCAT CCCCTCGGTG
    GGAGTGGTGA ACCCCATCCT GGCCAGCCCT CCAACGCTGG GTCTCCTGGA GCCCAAGAAG
    GAGAAGGAAG AAGAGGAGCT GTTTCCCGAG TCAGAGCGGC CAGAGATGCT GAGCGAGCAG
    GAGCACATGA GCATCTCGGG CAGTAGCGCC CGACACATGG TGATGCAGAA GCTGCTCCGC
    AAGCAGGAGT CTACAGTGAT GGTTCTGCGC AACATGGTGG ACCCCAAGGA CATCGATGAT
    GACCTGGAAG GGGAGGTGAC AGAGGAGTGT GGCAAGTTCG GGGCCGTGAA CCGCGTCATC
    ATCTACCAAG AGAAACAAGG CGAGGAGGAG GATGCAGAAA TCATTGTCAA GATCTTTGTG
    GAGTTTTCCA TAGCCTCTGA GACTCATAAG GCCATCCAGG CCCTCAATGG CCGCTGGTTT
    GCTGGCCGCA AGGTGGTGGC TGAAGTGTAC GACCAGGAGC GTTTTGATAA CAGTGACCTC
    TCTGCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu22582
    Clone ID Related Accession (Same CDS sequence) NM_001136033.2 , NM_001136033.3 , XM_017013239.1
    Accession Version NM_001136033.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1551bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-10-20
    Organism Homo sapiens(human)
    Product poly(U)-binding-splicing factor PUF60 isoform c
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC234917.3, BG771821.1, BC011265.1 and AW249619.1. On Sep 1, 2012 this sequence version replaced NM_001136033.1. Transcript Variant: This variant (3) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at an alternate in-frame downstream start site, compared to variant 1. The encoded isoform (c) has a shorter N-terminus, compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC011265.1, SRR1660805.18195.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGGAGAACG GGCAGAGCAC AGCCGCCAAG CTGGGGCTGC CTCCCCTGAC GCCCGAGCAG 
    CAGGAGGCCC TTCAGAAGGC CAAGAAGTAC GCCATGGAGC AGAGCATCAA GAGTGTGCTG
    GTGAAGCAGA CCATCGCGCA CCAGCAGCAG CAGCTCACCA ACCTGCAGAT GGCAGCAGTG
    ACAATGGGCT TTGGAGATCC TCTCTCACCT TTGCAATCGA TGGCGGCTCA GCGGCAGCGG
    GCGCTGGCCA TCATGTGCCG CGTCTACGTG GGCTCTATCT ACTATGAGCT GGGGGAGGAC
    ACCATCCGCC AGGCCTTTGC CCCCTTTGGC CCCATCAAGA GCATCGACAT GTCCTGGGAC
    TCCGTCACCA TGAAGCACAA GGGCTTTGCC TTCGTGGAGT ATGAGGTCCC CGAAGCTGCA
    CAGCTGGCCT TGGAGCAGAT GAACTCGGTG ATGCTGGGGG GCAGGAACAT CAAGGTGGGC
    AGACCCAGCA ACATAGGGCA GGCCCAGCCC ATCATAGACC AGTTGGCTGA GGAGGCACGG
    GCCTTCAACC GCATCTACGT GGCCTCTGTG CACCAGGACC TCTCAGACGA TGACATCAAG
    AGCGTGTTTG AGGCCTTTGG CAAGATCAAG TCCTGCACAC TGGCCCGGGA CCCCACAACT
    GGCAAGCACA AGGGCTACGG CTTCATTGAG TACGAGAAGG CCCAGTCGTC CCAAGATGCT
    GTGTCTTCCA TGAACCTCTT TGACCTGGGT GGCCAGTACT TGCGGGTGGG CAAGGCTGTC
    ACACCGCCCA TGCCCCTACT CACACCAGCC ACGCCTGGAG GCCTCCCACC TGCCGCTGCT
    GTGGCAGCTG CTGCAGCCAC TGCCAAGATC ACAGCTCAGG AAGCAGTGGC CGGAGCAGCG
    GTGCTGGGTA CCCTGGGCAC ACCTGGACTG GTGTCCCCAG CACTGACCCT GGCCCAGCCC
    CTGGGCACTT TGCCCCAGGC TGTCATGGCT GCCCAGGCAC CTGGAGTCAT CACAGGTGTG
    ACCCCAGCCC GTCCTCCTAT CCCGGTCACC ATCCCCTCGG TGGGAGTGGT GAACCCCATC
    CTGGCCAGCC CTCCAACGCT GGGTCTCCTG GAGCCCAAGA AGGAGAAGGA AGAAGAGGAG
    CTGTTTCCCG AGTCAGAGCG GCCAGAGATG CTGAGCGAGC AGGAGCACAT GAGCATCTCG
    GGCAGTAGCG CCCGACACAT GGTGATGCAG AAGCTGCTCC GCAAGCAGGA GTCTACAGTG
    ATGGTTCTGC GCAACATGGT GGACCCCAAG GACATCGATG ATGACCTGGA AGGGGAGGTG
    ACAGAGGAGT GTGGCAAGTT CGGGGCCGTG AACCGCGTCA TCATCTACCA AGAGAAACAA
    GGCGAGGAGG AGGATGCAGA AATCATTGTC AAGATCTTTG TGGAGTTTTC CATAGCCTCT
    GAGACTCATA AGGCCATCCA GGCCCTCAAT GGCCGCTGGT TTGCTGGCCG CAAGGTGGTG
    GCTGAAGTGT ACGACCAGGA GCGTTTTGAT AACAGTGACC TCTCTGCGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001129505.1
    CDS232..1782
    Translation

    Target ORF information:

    RefSeq Version NM_001136033.2
    Organism Homo sapiens(human)
    Definition Homo sapiens poly(U) binding splicing factor 60 (PUF60), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001136033.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGGAGAACG GGCAGAGCAC AGCCGCCAAG CTGGGGCTGC CTCCCCTGAC GCCCGAGCAG 
    CAGGAGGCCC TTCAGAAGGC CAAGAAGTAC GCCATGGAGC AGAGCATCAA GAGTGTGCTG
    GTGAAGCAGA CCATCGCGCA CCAGCAGCAG CAGCTCACCA ACCTGCAGAT GGCAGCAGTG
    ACAATGGGCT TTGGAGATCC TCTCTCACCT TTGCAATCGA TGGCGGCTCA GCGGCAGCGG
    GCGCTGGCCA TCATGTGCCG CGTCTACGTG GGCTCTATCT ACTATGAGCT GGGGGAGGAC
    ACCATCCGCC AGGCCTTTGC CCCCTTTGGC CCCATCAAGA GCATCGACAT GTCCTGGGAC
    TCCGTCACCA TGAAGCACAA GGGCTTTGCC TTCGTGGAGT ATGAGGTCCC CGAAGCTGCA
    CAGCTGGCCT TGGAGCAGAT GAACTCGGTG ATGCTGGGGG GCAGGAACAT CAAGGTGGGC
    AGACCCAGCA ACATAGGGCA GGCCCAGCCC ATCATAGACC AGTTGGCTGA GGAGGCACGG
    GCCTTCAACC GCATCTACGT GGCCTCTGTG CACCAGGACC TCTCAGACGA TGACATCAAG
    AGCGTGTTTG AGGCCTTTGG CAAGATCAAG TCCTGCACAC TGGCCCGGGA CCCCACAACT
    GGCAAGCACA AGGGCTACGG CTTCATTGAG TACGAGAAGG CCCAGTCGTC CCAAGATGCT
    GTGTCTTCCA TGAACCTCTT TGACCTGGGT GGCCAGTACT TGCGGGTGGG CAAGGCTGTC
    ACACCGCCCA TGCCCCTACT CACACCAGCC ACGCCTGGAG GCCTCCCACC TGCCGCTGCT
    GTGGCAGCTG CTGCAGCCAC TGCCAAGATC ACAGCTCAGG AAGCAGTGGC CGGAGCAGCG
    GTGCTGGGTA CCCTGGGCAC ACCTGGACTG GTGTCCCCAG CACTGACCCT GGCCCAGCCC
    CTGGGCACTT TGCCCCAGGC TGTCATGGCT GCCCAGGCAC CTGGAGTCAT CACAGGTGTG
    ACCCCAGCCC GTCCTCCTAT CCCGGTCACC ATCCCCTCGG TGGGAGTGGT GAACCCCATC
    CTGGCCAGCC CTCCAACGCT GGGTCTCCTG GAGCCCAAGA AGGAGAAGGA AGAAGAGGAG
    CTGTTTCCCG AGTCAGAGCG GCCAGAGATG CTGAGCGAGC AGGAGCACAT GAGCATCTCG
    GGCAGTAGCG CCCGACACAT GGTGATGCAG AAGCTGCTCC GCAAGCAGGA GTCTACAGTG
    ATGGTTCTGC GCAACATGGT GGACCCCAAG GACATCGATG ATGACCTGGA AGGGGAGGTG
    ACAGAGGAGT GTGGCAAGTT CGGGGCCGTG AACCGCGTCA TCATCTACCA AGAGAAACAA
    GGCGAGGAGG AGGATGCAGA AATCATTGTC AAGATCTTTG TGGAGTTTTC CATAGCCTCT
    GAGACTCATA AGGCCATCCA GGCCCTCAAT GGCCGCTGGT TTGCTGGCCG CAAGGTGGTG
    GCTGAAGTGT ACGACCAGGA GCGTTTTGAT AACAGTGACC TCTCTGCGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu22582
    Clone ID Related Accession (Same CDS sequence) NM_001136033.2 , NM_001136033.3 , XM_017013239.1
    Accession Version NM_001136033.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1551bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-27
    Organism Homo sapiens(human)
    Product poly(U)-binding-splicing factor PUF60 isoform c
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BG771821.1, BC011265.1 and AW249619.1. On May 31, 2019 this sequence version replaced NM_001136033.2. Transcript Variant: This variant (3) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at an alternate in-frame downstream start site, compared to variant 1. The encoded isoform (c) has a shorter N-terminus, compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR3476690.1022935.1, SRR7410570.432098.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGGAGAACG GGCAGAGCAC AGCCGCCAAG CTGGGGCTGC CTCCCCTGAC GCCCGAGCAG 
    CAGGAGGCCC TTCAGAAGGC CAAGAAGTAC GCCATGGAGC AGAGCATCAA GAGTGTGCTG
    GTGAAGCAGA CCATCGCGCA CCAGCAGCAG CAGCTCACCA ACCTGCAGAT GGCAGCAGTG
    ACAATGGGCT TTGGAGATCC TCTCTCACCT TTGCAATCGA TGGCGGCTCA GCGGCAGCGG
    GCGCTGGCCA TCATGTGCCG CGTCTACGTG GGCTCTATCT ACTATGAGCT GGGGGAGGAC
    ACCATCCGCC AGGCCTTTGC CCCCTTTGGC CCCATCAAGA GCATCGACAT GTCCTGGGAC
    TCCGTCACCA TGAAGCACAA GGGCTTTGCC TTCGTGGAGT ATGAGGTCCC CGAAGCTGCA
    CAGCTGGCCT TGGAGCAGAT GAACTCGGTG ATGCTGGGGG GCAGGAACAT CAAGGTGGGC
    AGACCCAGCA ACATAGGGCA GGCCCAGCCC ATCATAGACC AGTTGGCTGA GGAGGCACGG
    GCCTTCAACC GCATCTACGT GGCCTCTGTG CACCAGGACC TCTCAGACGA TGACATCAAG
    AGCGTGTTTG AGGCCTTTGG CAAGATCAAG TCCTGCACAC TGGCCCGGGA CCCCACAACT
    GGCAAGCACA AGGGCTACGG CTTCATTGAG TACGAGAAGG CCCAGTCGTC CCAAGATGCT
    GTGTCTTCCA TGAACCTCTT TGACCTGGGT GGCCAGTACT TGCGGGTGGG CAAGGCTGTC
    ACACCGCCCA TGCCCCTACT CACACCAGCC ACGCCTGGAG GCCTCCCACC TGCCGCTGCT
    GTGGCAGCTG CTGCAGCCAC TGCCAAGATC ACAGCTCAGG AAGCAGTGGC CGGAGCAGCG
    GTGCTGGGTA CCCTGGGCAC ACCTGGACTG GTGTCCCCAG CACTGACCCT GGCCCAGCCC
    CTGGGCACTT TGCCCCAGGC TGTCATGGCT GCCCAGGCAC CTGGAGTCAT CACAGGTGTG
    ACCCCAGCCC GTCCTCCTAT CCCGGTCACC ATCCCCTCGG TGGGAGTGGT GAACCCCATC
    CTGGCCAGCC CTCCAACGCT GGGTCTCCTG GAGCCCAAGA AGGAGAAGGA AGAAGAGGAG
    CTGTTTCCCG AGTCAGAGCG GCCAGAGATG CTGAGCGAGC AGGAGCACAT GAGCATCTCG
    GGCAGTAGCG CCCGACACAT GGTGATGCAG AAGCTGCTCC GCAAGCAGGA GTCTACAGTG
    ATGGTTCTGC GCAACATGGT GGACCCCAAG GACATCGATG ATGACCTGGA AGGGGAGGTG
    ACAGAGGAGT GTGGCAAGTT CGGGGCCGTG AACCGCGTCA TCATCTACCA AGAGAAACAA
    GGCGAGGAGG AGGATGCAGA AATCATTGTC AAGATCTTTG TGGAGTTTTC CATAGCCTCT
    GAGACTCATA AGGCCATCCA GGCCCTCAAT GGCCGCTGGT TTGCTGGCCG CAAGGTGGTG
    GCTGAAGTGT ACGACCAGGA GCGTTTTGAT AACAGTGACC TCTCTGCGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001129505.1
    CDS164..1714
    Translation

    Target ORF information:

    RefSeq Version NM_001136033.3
    Organism Homo sapiens(human)
    Definition Homo sapiens poly(U) binding splicing factor 60 (PUF60), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001136033.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGGAGAACG GGCAGAGCAC AGCCGCCAAG CTGGGGCTGC CTCCCCTGAC GCCCGAGCAG 
    CAGGAGGCCC TTCAGAAGGC CAAGAAGTAC GCCATGGAGC AGAGCATCAA GAGTGTGCTG
    GTGAAGCAGA CCATCGCGCA CCAGCAGCAG CAGCTCACCA ACCTGCAGAT GGCAGCAGTG
    ACAATGGGCT TTGGAGATCC TCTCTCACCT TTGCAATCGA TGGCGGCTCA GCGGCAGCGG
    GCGCTGGCCA TCATGTGCCG CGTCTACGTG GGCTCTATCT ACTATGAGCT GGGGGAGGAC
    ACCATCCGCC AGGCCTTTGC CCCCTTTGGC CCCATCAAGA GCATCGACAT GTCCTGGGAC
    TCCGTCACCA TGAAGCACAA GGGCTTTGCC TTCGTGGAGT ATGAGGTCCC CGAAGCTGCA
    CAGCTGGCCT TGGAGCAGAT GAACTCGGTG ATGCTGGGGG GCAGGAACAT CAAGGTGGGC
    AGACCCAGCA ACATAGGGCA GGCCCAGCCC ATCATAGACC AGTTGGCTGA GGAGGCACGG
    GCCTTCAACC GCATCTACGT GGCCTCTGTG CACCAGGACC TCTCAGACGA TGACATCAAG
    AGCGTGTTTG AGGCCTTTGG CAAGATCAAG TCCTGCACAC TGGCCCGGGA CCCCACAACT
    GGCAAGCACA AGGGCTACGG CTTCATTGAG TACGAGAAGG CCCAGTCGTC CCAAGATGCT
    GTGTCTTCCA TGAACCTCTT TGACCTGGGT GGCCAGTACT TGCGGGTGGG CAAGGCTGTC
    ACACCGCCCA TGCCCCTACT CACACCAGCC ACGCCTGGAG GCCTCCCACC TGCCGCTGCT
    GTGGCAGCTG CTGCAGCCAC TGCCAAGATC ACAGCTCAGG AAGCAGTGGC CGGAGCAGCG
    GTGCTGGGTA CCCTGGGCAC ACCTGGACTG GTGTCCCCAG CACTGACCCT GGCCCAGCCC
    CTGGGCACTT TGCCCCAGGC TGTCATGGCT GCCCAGGCAC CTGGAGTCAT CACAGGTGTG
    ACCCCAGCCC GTCCTCCTAT CCCGGTCACC ATCCCCTCGG TGGGAGTGGT GAACCCCATC
    CTGGCCAGCC CTCCAACGCT GGGTCTCCTG GAGCCCAAGA AGGAGAAGGA AGAAGAGGAG
    CTGTTTCCCG AGTCAGAGCG GCCAGAGATG CTGAGCGAGC AGGAGCACAT GAGCATCTCG
    GGCAGTAGCG CCCGACACAT GGTGATGCAG AAGCTGCTCC GCAAGCAGGA GTCTACAGTG
    ATGGTTCTGC GCAACATGGT GGACCCCAAG GACATCGATG ATGACCTGGA AGGGGAGGTG
    ACAGAGGAGT GTGGCAAGTT CGGGGCCGTG AACCGCGTCA TCATCTACCA AGAGAAACAA
    GGCGAGGAGG AGGATGCAGA AATCATTGTC AAGATCTTTG TGGAGTTTTC CATAGCCTCT
    GAGACTCATA AGGCCATCCA GGCCCTCAAT GGCCGCTGGT TTGCTGGCCG CAAGGTGGTG
    GCTGAAGTGT ACGACCAGGA GCGTTTTGAT AACAGTGACC TCTCTGCGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu23020
    Clone ID Related Accession (Same CDS sequence) NM_001271100.1 , NM_001271100.2 , XM_017013240.1
    Accession Version NM_001271100.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1500bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-10-20
    Organism Homo sapiens(human)
    Product poly(U)-binding-splicing factor PUF60 isoform h
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC234917.3, BG771821.1, BC009734.1 and AW249619.1. Transcript Variant: This variant (8) differs in the 5' UTR and contains multiple differences in the coding region, compared to variant 1. These differences result in initiation of translation at an alternate in-frame downstream start site. The encoded isoform (h) is shorter than isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC009734.1, SRR1803611.179234.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGGAGAACG GGCAGAGCAC AGCCGCCAAG CTGGGGCTGC CTCCCCTGAC GCCCGAGCAG 
    CAGGAGGCCC TTCAGAAGGC CAAGAAGTAC GCCATGGAGC AGAGCATCAA GAGTGTGCTG
    GTGAAGCAGA CCATCGCGCA CCAGCAGCAG CAGCTCACCA ACCTGCAGAT GGCGGCTCAG
    CGGCAGCGGG CGCTGGCCAT CATGTGCCGC GTCTACGTGG GCTCTATCTA CTATGAGCTG
    GGGGAGGACA CCATCCGCCA GGCCTTTGCC CCCTTTGGCC CCATCAAGAG CATCGACATG
    TCCTGGGACT CCGTCACCAT GAAGCACAAG GGCTTTGCCT TCGTGGAGTA TGAGGTCCCC
    GAAGCTGCAC AGCTGGCCTT GGAGCAGATG AACTCGGTGA TGCTGGGGGG CAGGAACATC
    AAGGTGGGCA GACCCAGCAA CATAGGGCAG GCCCAGCCCA TCATAGACCA GTTGGCTGAG
    GAGGCACGGG CCTTCAACCG CATCTACGTG GCCTCTGTGC ACCAGGACCT CTCAGACGAT
    GACATCAAGA GCGTGTTTGA GGCCTTTGGC AAGATCAAGT CCTGCACACT GGCCCGGGAC
    CCCACAACTG GCAAGCACAA GGGCTACGGC TTCATTGAGT ACGAGAAGGC CCAGTCGTCC
    CAAGATGCTG TGTCTTCCAT GAACCTCTTT GACCTGGGTG GCCAGTACTT GCGGGTGGGC
    AAGGCTGTCA CACCGCCCAT GCCCCTACTC ACACCAGCCA CGCCTGGAGG CCTCCCACCT
    GCCGCTGCTG TGGCAGCTGC TGCAGCCACT GCCAAGATCA CAGCTCAGGA AGCAGTGGCC
    GGAGCAGCGG TGCTGGGTAC CCTGGGCACA CCTGGACTGG TGTCCCCAGC ACTGACCCTG
    GCCCAGCCCC TGGGCACTTT GCCCCAGGCT GTCATGGCTG CCCAGGCACC TGGAGTCATC
    ACAGGTGTGA CCCCAGCCCG TCCTCCTATC CCGGTCACCA TCCCCTCGGT GGGAGTGGTG
    AACCCCATCC TGGCCAGCCC TCCAACGCTG GGTCTCCTGG AGCCCAAGAA GGAGAAGGAA
    GAAGAGGAGC TGTTTCCCGA GTCAGAGCGG CCAGAGATGC TGAGCGAGCA GGAGCACATG
    AGCATCTCGG GCAGTAGCGC CCGACACATG GTGATGCAGA AGCTGCTCCG CAAGCAGGAG
    TCTACAGTGA TGGTTCTGCG CAACATGGTG GACCCCAAGG ACATCGATGA TGACCTGGAA
    GGGGAGGTGA CAGAGGAGTG TGGCAAGTTC GGGGCCGTGA ACCGCGTCAT CATCTACCAA
    GAGAAACAAG GCGAGGAGGA GGATGCAGAA ATCATTGTCA AGATCTTTGT GGAGTTTTCC
    ATAGCCTCTG AGACTCATAA GGCCATCCAG GCCCTCAATG GCCGCTGGTT TGCTGGCCGC
    AAGGTGGTGG CTGAAGTGTA CGACCAGGAG CGTTTTGATA ACAGTGACCT CTCTGCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001258029.1
    CDS232..1731
    Translation

    Target ORF information:

    RefSeq Version NM_001271100.1
    Organism Homo sapiens(human)
    Definition Homo sapiens poly(U) binding splicing factor 60 (PUF60), transcript variant 8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001271100.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGGAGAACG GGCAGAGCAC AGCCGCCAAG CTGGGGCTGC CTCCCCTGAC GCCCGAGCAG 
    CAGGAGGCCC TTCAGAAGGC CAAGAAGTAC GCCATGGAGC AGAGCATCAA GAGTGTGCTG
    GTGAAGCAGA CCATCGCGCA CCAGCAGCAG CAGCTCACCA ACCTGCAGAT GGCGGCTCAG
    CGGCAGCGGG CGCTGGCCAT CATGTGCCGC GTCTACGTGG GCTCTATCTA CTATGAGCTG
    GGGGAGGACA CCATCCGCCA GGCCTTTGCC CCCTTTGGCC CCATCAAGAG CATCGACATG
    TCCTGGGACT CCGTCACCAT GAAGCACAAG GGCTTTGCCT TCGTGGAGTA TGAGGTCCCC
    GAAGCTGCAC AGCTGGCCTT GGAGCAGATG AACTCGGTGA TGCTGGGGGG CAGGAACATC
    AAGGTGGGCA GACCCAGCAA CATAGGGCAG GCCCAGCCCA TCATAGACCA GTTGGCTGAG
    GAGGCACGGG CCTTCAACCG CATCTACGTG GCCTCTGTGC ACCAGGACCT CTCAGACGAT
    GACATCAAGA GCGTGTTTGA GGCCTTTGGC AAGATCAAGT CCTGCACACT GGCCCGGGAC
    CCCACAACTG GCAAGCACAA GGGCTACGGC TTCATTGAGT ACGAGAAGGC CCAGTCGTCC
    CAAGATGCTG TGTCTTCCAT GAACCTCTTT GACCTGGGTG GCCAGTACTT GCGGGTGGGC
    AAGGCTGTCA CACCGCCCAT GCCCCTACTC ACACCAGCCA CGCCTGGAGG CCTCCCACCT
    GCCGCTGCTG TGGCAGCTGC TGCAGCCACT GCCAAGATCA CAGCTCAGGA AGCAGTGGCC
    GGAGCAGCGG TGCTGGGTAC CCTGGGCACA CCTGGACTGG TGTCCCCAGC ACTGACCCTG
    GCCCAGCCCC TGGGCACTTT GCCCCAGGCT GTCATGGCTG CCCAGGCACC TGGAGTCATC
    ACAGGTGTGA CCCCAGCCCG TCCTCCTATC CCGGTCACCA TCCCCTCGGT GGGAGTGGTG
    AACCCCATCC TGGCCAGCCC TCCAACGCTG GGTCTCCTGG AGCCCAAGAA GGAGAAGGAA
    GAAGAGGAGC TGTTTCCCGA GTCAGAGCGG CCAGAGATGC TGAGCGAGCA GGAGCACATG
    AGCATCTCGG GCAGTAGCGC CCGACACATG GTGATGCAGA AGCTGCTCCG CAAGCAGGAG
    TCTACAGTGA TGGTTCTGCG CAACATGGTG GACCCCAAGG ACATCGATGA TGACCTGGAA
    GGGGAGGTGA CAGAGGAGTG TGGCAAGTTC GGGGCCGTGA ACCGCGTCAT CATCTACCAA
    GAGAAACAAG GCGAGGAGGA GGATGCAGAA ATCATTGTCA AGATCTTTGT GGAGTTTTCC
    ATAGCCTCTG AGACTCATAA GGCCATCCAG GCCCTCAATG GCCGCTGGTT TGCTGGCCGC
    AAGGTGGTGG CTGAAGTGTA CGACCAGGAG CGTTTTGATA ACAGTGACCT CTCTGCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu23020
    Clone ID Related Accession (Same CDS sequence) NM_001271100.1 , NM_001271100.2 , XM_017013240.1
    Accession Version NM_001271100.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1500bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-27
    Organism Homo sapiens(human)
    Product poly(U)-binding-splicing factor PUF60 isoform h
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BG771821.1, BC009734.1 and AW249619.1. On May 31, 2019 this sequence version replaced NM_001271100.1. Transcript Variant: This variant (8) differs in the 5' UTR and contains multiple differences in the coding region, compared to variant 1. These differences result in initiation of translation at an alternate in-frame downstream start site. The encoded isoform (h) is shorter than isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR7410570.432094.1, BC009734.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGGAGAACG GGCAGAGCAC AGCCGCCAAG CTGGGGCTGC CTCCCCTGAC GCCCGAGCAG 
    CAGGAGGCCC TTCAGAAGGC CAAGAAGTAC GCCATGGAGC AGAGCATCAA GAGTGTGCTG
    GTGAAGCAGA CCATCGCGCA CCAGCAGCAG CAGCTCACCA ACCTGCAGAT GGCGGCTCAG
    CGGCAGCGGG CGCTGGCCAT CATGTGCCGC GTCTACGTGG GCTCTATCTA CTATGAGCTG
    GGGGAGGACA CCATCCGCCA GGCCTTTGCC CCCTTTGGCC CCATCAAGAG CATCGACATG
    TCCTGGGACT CCGTCACCAT GAAGCACAAG GGCTTTGCCT TCGTGGAGTA TGAGGTCCCC
    GAAGCTGCAC AGCTGGCCTT GGAGCAGATG AACTCGGTGA TGCTGGGGGG CAGGAACATC
    AAGGTGGGCA GACCCAGCAA CATAGGGCAG GCCCAGCCCA TCATAGACCA GTTGGCTGAG
    GAGGCACGGG CCTTCAACCG CATCTACGTG GCCTCTGTGC ACCAGGACCT CTCAGACGAT
    GACATCAAGA GCGTGTTTGA GGCCTTTGGC AAGATCAAGT CCTGCACACT GGCCCGGGAC
    CCCACAACTG GCAAGCACAA GGGCTACGGC TTCATTGAGT ACGAGAAGGC CCAGTCGTCC
    CAAGATGCTG TGTCTTCCAT GAACCTCTTT GACCTGGGTG GCCAGTACTT GCGGGTGGGC
    AAGGCTGTCA CACCGCCCAT GCCCCTACTC ACACCAGCCA CGCCTGGAGG CCTCCCACCT
    GCCGCTGCTG TGGCAGCTGC TGCAGCCACT GCCAAGATCA CAGCTCAGGA AGCAGTGGCC
    GGAGCAGCGG TGCTGGGTAC CCTGGGCACA CCTGGACTGG TGTCCCCAGC ACTGACCCTG
    GCCCAGCCCC TGGGCACTTT GCCCCAGGCT GTCATGGCTG CCCAGGCACC TGGAGTCATC
    ACAGGTGTGA CCCCAGCCCG TCCTCCTATC CCGGTCACCA TCCCCTCGGT GGGAGTGGTG
    AACCCCATCC TGGCCAGCCC TCCAACGCTG GGTCTCCTGG AGCCCAAGAA GGAGAAGGAA
    GAAGAGGAGC TGTTTCCCGA GTCAGAGCGG CCAGAGATGC TGAGCGAGCA GGAGCACATG
    AGCATCTCGG GCAGTAGCGC CCGACACATG GTGATGCAGA AGCTGCTCCG CAAGCAGGAG
    TCTACAGTGA TGGTTCTGCG CAACATGGTG GACCCCAAGG ACATCGATGA TGACCTGGAA
    GGGGAGGTGA CAGAGGAGTG TGGCAAGTTC GGGGCCGTGA ACCGCGTCAT CATCTACCAA
    GAGAAACAAG GCGAGGAGGA GGATGCAGAA ATCATTGTCA AGATCTTTGT GGAGTTTTCC
    ATAGCCTCTG AGACTCATAA GGCCATCCAG GCCCTCAATG GCCGCTGGTT TGCTGGCCGC
    AAGGTGGTGG CTGAAGTGTA CGACCAGGAG CGTTTTGATA ACAGTGACCT CTCTGCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001258029.1
    CDS164..1663
    Translation

    Target ORF information:

    RefSeq Version NM_001271100.2
    Organism Homo sapiens(human)
    Definition Homo sapiens poly(U) binding splicing factor 60 (PUF60), transcript variant 8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001271100.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGGAGAACG GGCAGAGCAC AGCCGCCAAG CTGGGGCTGC CTCCCCTGAC GCCCGAGCAG 
    CAGGAGGCCC TTCAGAAGGC CAAGAAGTAC GCCATGGAGC AGAGCATCAA GAGTGTGCTG
    GTGAAGCAGA CCATCGCGCA CCAGCAGCAG CAGCTCACCA ACCTGCAGAT GGCGGCTCAG
    CGGCAGCGGG CGCTGGCCAT CATGTGCCGC GTCTACGTGG GCTCTATCTA CTATGAGCTG
    GGGGAGGACA CCATCCGCCA GGCCTTTGCC CCCTTTGGCC CCATCAAGAG CATCGACATG
    TCCTGGGACT CCGTCACCAT GAAGCACAAG GGCTTTGCCT TCGTGGAGTA TGAGGTCCCC
    GAAGCTGCAC AGCTGGCCTT GGAGCAGATG AACTCGGTGA TGCTGGGGGG CAGGAACATC
    AAGGTGGGCA GACCCAGCAA CATAGGGCAG GCCCAGCCCA TCATAGACCA GTTGGCTGAG
    GAGGCACGGG CCTTCAACCG CATCTACGTG GCCTCTGTGC ACCAGGACCT CTCAGACGAT
    GACATCAAGA GCGTGTTTGA GGCCTTTGGC AAGATCAAGT CCTGCACACT GGCCCGGGAC
    CCCACAACTG GCAAGCACAA GGGCTACGGC TTCATTGAGT ACGAGAAGGC CCAGTCGTCC
    CAAGATGCTG TGTCTTCCAT GAACCTCTTT GACCTGGGTG GCCAGTACTT GCGGGTGGGC
    AAGGCTGTCA CACCGCCCAT GCCCCTACTC ACACCAGCCA CGCCTGGAGG CCTCCCACCT
    GCCGCTGCTG TGGCAGCTGC TGCAGCCACT GCCAAGATCA CAGCTCAGGA AGCAGTGGCC
    GGAGCAGCGG TGCTGGGTAC CCTGGGCACA CCTGGACTGG TGTCCCCAGC ACTGACCCTG
    GCCCAGCCCC TGGGCACTTT GCCCCAGGCT GTCATGGCTG CCCAGGCACC TGGAGTCATC
    ACAGGTGTGA CCCCAGCCCG TCCTCCTATC CCGGTCACCA TCCCCTCGGT GGGAGTGGTG
    AACCCCATCC TGGCCAGCCC TCCAACGCTG GGTCTCCTGG AGCCCAAGAA GGAGAAGGAA
    GAAGAGGAGC TGTTTCCCGA GTCAGAGCGG CCAGAGATGC TGAGCGAGCA GGAGCACATG
    AGCATCTCGG GCAGTAGCGC CCGACACATG GTGATGCAGA AGCTGCTCCG CAAGCAGGAG
    TCTACAGTGA TGGTTCTGCG CAACATGGTG GACCCCAAGG ACATCGATGA TGACCTGGAA
    GGGGAGGTGA CAGAGGAGTG TGGCAAGTTC GGGGCCGTGA ACCGCGTCAT CATCTACCAA
    GAGAAACAAG GCGAGGAGGA GGATGCAGAA ATCATTGTCA AGATCTTTGT GGAGTTTTCC
    ATAGCCTCTG AGACTCATAA GGCCATCCAG GCCCTCAATG GCCGCTGGTT TGCTGGCCGC
    AAGGTGGTGG CTGAAGTGTA CGACCAGGAG CGTTTTGATA ACAGTGACCT CTCTGCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu22582
    Clone ID Related Accession (Same CDS sequence) NM_001136033.2 , NM_001136033.3 , XM_017013239.1
    Accession Version XM_017013239.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1551bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product poly(U)-binding-splicing factor PUF60 isoform X13
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000008.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGGAGAACG GGCAGAGCAC AGCCGCCAAG CTGGGGCTGC CTCCCCTGAC GCCCGAGCAG 
    CAGGAGGCCC TTCAGAAGGC CAAGAAGTAC GCCATGGAGC AGAGCATCAA GAGTGTGCTG
    GTGAAGCAGA CCATCGCGCA CCAGCAGCAG CAGCTCACCA ACCTGCAGAT GGCAGCAGTG
    ACAATGGGCT TTGGAGATCC TCTCTCACCT TTGCAATCGA TGGCGGCTCA GCGGCAGCGG
    GCGCTGGCCA TCATGTGCCG CGTCTACGTG GGCTCTATCT ACTATGAGCT GGGGGAGGAC
    ACCATCCGCC AGGCCTTTGC CCCCTTTGGC CCCATCAAGA GCATCGACAT GTCCTGGGAC
    TCCGTCACCA TGAAGCACAA GGGCTTTGCC TTCGTGGAGT ATGAGGTCCC CGAAGCTGCA
    CAGCTGGCCT TGGAGCAGAT GAACTCGGTG ATGCTGGGGG GCAGGAACAT CAAGGTGGGC
    AGACCCAGCA ACATAGGGCA GGCCCAGCCC ATCATAGACC AGTTGGCTGA GGAGGCACGG
    GCCTTCAACC GCATCTACGT GGCCTCTGTG CACCAGGACC TCTCAGACGA TGACATCAAG
    AGCGTGTTTG AGGCCTTTGG CAAGATCAAG TCCTGCACAC TGGCCCGGGA CCCCACAACT
    GGCAAGCACA AGGGCTACGG CTTCATTGAG TACGAGAAGG CCCAGTCGTC CCAAGATGCT
    GTGTCTTCCA TGAACCTCTT TGACCTGGGT GGCCAGTACT TGCGGGTGGG CAAGGCTGTC
    ACACCGCCCA TGCCCCTACT CACACCAGCC ACGCCTGGAG GCCTCCCACC TGCCGCTGCT
    GTGGCAGCTG CTGCAGCCAC TGCCAAGATC ACAGCTCAGG AAGCAGTGGC CGGAGCAGCG
    GTGCTGGGTA CCCTGGGCAC ACCTGGACTG GTGTCCCCAG CACTGACCCT GGCCCAGCCC
    CTGGGCACTT TGCCCCAGGC TGTCATGGCT GCCCAGGCAC CTGGAGTCAT CACAGGTGTG
    ACCCCAGCCC GTCCTCCTAT CCCGGTCACC ATCCCCTCGG TGGGAGTGGT GAACCCCATC
    CTGGCCAGCC CTCCAACGCT GGGTCTCCTG GAGCCCAAGA AGGAGAAGGA AGAAGAGGAG
    CTGTTTCCCG AGTCAGAGCG GCCAGAGATG CTGAGCGAGC AGGAGCACAT GAGCATCTCG
    GGCAGTAGCG CCCGACACAT GGTGATGCAG AAGCTGCTCC GCAAGCAGGA GTCTACAGTG
    ATGGTTCTGC GCAACATGGT GGACCCCAAG GACATCGATG ATGACCTGGA AGGGGAGGTG
    ACAGAGGAGT GTGGCAAGTT CGGGGCCGTG AACCGCGTCA TCATCTACCA AGAGAAACAA
    GGCGAGGAGG AGGATGCAGA AATCATTGTC AAGATCTTTG TGGAGTTTTC CATAGCCTCT
    GAGACTCATA AGGCCATCCA GGCCCTCAAT GGCCGCTGGT TTGCTGGCCG CAAGGTGGTG
    GCTGAAGTGT ACGACCAGGA GCGTTTTGAT AACAGTGACC TCTCTGCGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016868728.1
    CDS240..1790
    Translation

    Target ORF information:

    RefSeq Version XM_017013239.1
    Organism Homo sapiens(human)
    Definition Homo sapiens poly(U) binding splicing factor 60 (PUF60), transcript variant X17, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017013239.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGGAGAACG GGCAGAGCAC AGCCGCCAAG CTGGGGCTGC CTCCCCTGAC GCCCGAGCAG 
    CAGGAGGCCC TTCAGAAGGC CAAGAAGTAC GCCATGGAGC AGAGCATCAA GAGTGTGCTG
    GTGAAGCAGA CCATCGCGCA CCAGCAGCAG CAGCTCACCA ACCTGCAGAT GGCAGCAGTG
    ACAATGGGCT TTGGAGATCC TCTCTCACCT TTGCAATCGA TGGCGGCTCA GCGGCAGCGG
    GCGCTGGCCA TCATGTGCCG CGTCTACGTG GGCTCTATCT ACTATGAGCT GGGGGAGGAC
    ACCATCCGCC AGGCCTTTGC CCCCTTTGGC CCCATCAAGA GCATCGACAT GTCCTGGGAC
    TCCGTCACCA TGAAGCACAA GGGCTTTGCC TTCGTGGAGT ATGAGGTCCC CGAAGCTGCA
    CAGCTGGCCT TGGAGCAGAT GAACTCGGTG ATGCTGGGGG GCAGGAACAT CAAGGTGGGC
    AGACCCAGCA ACATAGGGCA GGCCCAGCCC ATCATAGACC AGTTGGCTGA GGAGGCACGG
    GCCTTCAACC GCATCTACGT GGCCTCTGTG CACCAGGACC TCTCAGACGA TGACATCAAG
    AGCGTGTTTG AGGCCTTTGG CAAGATCAAG TCCTGCACAC TGGCCCGGGA CCCCACAACT
    GGCAAGCACA AGGGCTACGG CTTCATTGAG TACGAGAAGG CCCAGTCGTC CCAAGATGCT
    GTGTCTTCCA TGAACCTCTT TGACCTGGGT GGCCAGTACT TGCGGGTGGG CAAGGCTGTC
    ACACCGCCCA TGCCCCTACT CACACCAGCC ACGCCTGGAG GCCTCCCACC TGCCGCTGCT
    GTGGCAGCTG CTGCAGCCAC TGCCAAGATC ACAGCTCAGG AAGCAGTGGC CGGAGCAGCG
    GTGCTGGGTA CCCTGGGCAC ACCTGGACTG GTGTCCCCAG CACTGACCCT GGCCCAGCCC
    CTGGGCACTT TGCCCCAGGC TGTCATGGCT GCCCAGGCAC CTGGAGTCAT CACAGGTGTG
    ACCCCAGCCC GTCCTCCTAT CCCGGTCACC ATCCCCTCGG TGGGAGTGGT GAACCCCATC
    CTGGCCAGCC CTCCAACGCT GGGTCTCCTG GAGCCCAAGA AGGAGAAGGA AGAAGAGGAG
    CTGTTTCCCG AGTCAGAGCG GCCAGAGATG CTGAGCGAGC AGGAGCACAT GAGCATCTCG
    GGCAGTAGCG CCCGACACAT GGTGATGCAG AAGCTGCTCC GCAAGCAGGA GTCTACAGTG
    ATGGTTCTGC GCAACATGGT GGACCCCAAG GACATCGATG ATGACCTGGA AGGGGAGGTG
    ACAGAGGAGT GTGGCAAGTT CGGGGCCGTG AACCGCGTCA TCATCTACCA AGAGAAACAA
    GGCGAGGAGG AGGATGCAGA AATCATTGTC AAGATCTTTG TGGAGTTTTC CATAGCCTCT
    GAGACTCATA AGGCCATCCA GGCCCTCAAT GGCCGCTGGT TTGCTGGCCG CAAGGTGGTG
    GCTGAAGTGT ACGACCAGGA GCGTTTTGAT AACAGTGACC TCTCTGCGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu23020
    Clone ID Related Accession (Same CDS sequence) NM_001271100.1 , NM_001271100.2 , XM_017013240.1
    Accession Version XM_017013240.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1500bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product poly(U)-binding-splicing factor PUF60 isoform X14
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000008.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGGAGAACG GGCAGAGCAC AGCCGCCAAG CTGGGGCTGC CTCCCCTGAC GCCCGAGCAG 
    CAGGAGGCCC TTCAGAAGGC CAAGAAGTAC GCCATGGAGC AGAGCATCAA GAGTGTGCTG
    GTGAAGCAGA CCATCGCGCA CCAGCAGCAG CAGCTCACCA ACCTGCAGAT GGCGGCTCAG
    CGGCAGCGGG CGCTGGCCAT CATGTGCCGC GTCTACGTGG GCTCTATCTA CTATGAGCTG
    GGGGAGGACA CCATCCGCCA GGCCTTTGCC CCCTTTGGCC CCATCAAGAG CATCGACATG
    TCCTGGGACT CCGTCACCAT GAAGCACAAG GGCTTTGCCT TCGTGGAGTA TGAGGTCCCC
    GAAGCTGCAC AGCTGGCCTT GGAGCAGATG AACTCGGTGA TGCTGGGGGG CAGGAACATC
    AAGGTGGGCA GACCCAGCAA CATAGGGCAG GCCCAGCCCA TCATAGACCA GTTGGCTGAG
    GAGGCACGGG CCTTCAACCG CATCTACGTG GCCTCTGTGC ACCAGGACCT CTCAGACGAT
    GACATCAAGA GCGTGTTTGA GGCCTTTGGC AAGATCAAGT CCTGCACACT GGCCCGGGAC
    CCCACAACTG GCAAGCACAA GGGCTACGGC TTCATTGAGT ACGAGAAGGC CCAGTCGTCC
    CAAGATGCTG TGTCTTCCAT GAACCTCTTT GACCTGGGTG GCCAGTACTT GCGGGTGGGC
    AAGGCTGTCA CACCGCCCAT GCCCCTACTC ACACCAGCCA CGCCTGGAGG CCTCCCACCT
    GCCGCTGCTG TGGCAGCTGC TGCAGCCACT GCCAAGATCA CAGCTCAGGA AGCAGTGGCC
    GGAGCAGCGG TGCTGGGTAC CCTGGGCACA CCTGGACTGG TGTCCCCAGC ACTGACCCTG
    GCCCAGCCCC TGGGCACTTT GCCCCAGGCT GTCATGGCTG CCCAGGCACC TGGAGTCATC
    ACAGGTGTGA CCCCAGCCCG TCCTCCTATC CCGGTCACCA TCCCCTCGGT GGGAGTGGTG
    AACCCCATCC TGGCCAGCCC TCCAACGCTG GGTCTCCTGG AGCCCAAGAA GGAGAAGGAA
    GAAGAGGAGC TGTTTCCCGA GTCAGAGCGG CCAGAGATGC TGAGCGAGCA GGAGCACATG
    AGCATCTCGG GCAGTAGCGC CCGACACATG GTGATGCAGA AGCTGCTCCG CAAGCAGGAG
    TCTACAGTGA TGGTTCTGCG CAACATGGTG GACCCCAAGG ACATCGATGA TGACCTGGAA
    GGGGAGGTGA CAGAGGAGTG TGGCAAGTTC GGGGCCGTGA ACCGCGTCAT CATCTACCAA
    GAGAAACAAG GCGAGGAGGA GGATGCAGAA ATCATTGTCA AGATCTTTGT GGAGTTTTCC
    ATAGCCTCTG AGACTCATAA GGCCATCCAG GCCCTCAATG GCCGCTGGTT TGCTGGCCGC
    AAGGTGGTGG CTGAAGTGTA CGACCAGGAG CGTTTTGATA ACAGTGACCT CTCTGCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016868729.1
    CDS240..1739
    Translation

    Target ORF information:

    RefSeq Version XM_017013240.1
    Organism Homo sapiens(human)
    Definition Homo sapiens poly(U) binding splicing factor 60 (PUF60), transcript variant X18, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017013240.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGGAGAACG GGCAGAGCAC AGCCGCCAAG CTGGGGCTGC CTCCCCTGAC GCCCGAGCAG 
    CAGGAGGCCC TTCAGAAGGC CAAGAAGTAC GCCATGGAGC AGAGCATCAA GAGTGTGCTG
    GTGAAGCAGA CCATCGCGCA CCAGCAGCAG CAGCTCACCA ACCTGCAGAT GGCGGCTCAG
    CGGCAGCGGG CGCTGGCCAT CATGTGCCGC GTCTACGTGG GCTCTATCTA CTATGAGCTG
    GGGGAGGACA CCATCCGCCA GGCCTTTGCC CCCTTTGGCC CCATCAAGAG CATCGACATG
    TCCTGGGACT CCGTCACCAT GAAGCACAAG GGCTTTGCCT TCGTGGAGTA TGAGGTCCCC
    GAAGCTGCAC AGCTGGCCTT GGAGCAGATG AACTCGGTGA TGCTGGGGGG CAGGAACATC
    AAGGTGGGCA GACCCAGCAA CATAGGGCAG GCCCAGCCCA TCATAGACCA GTTGGCTGAG
    GAGGCACGGG CCTTCAACCG CATCTACGTG GCCTCTGTGC ACCAGGACCT CTCAGACGAT
    GACATCAAGA GCGTGTTTGA GGCCTTTGGC AAGATCAAGT CCTGCACACT GGCCCGGGAC
    CCCACAACTG GCAAGCACAA GGGCTACGGC TTCATTGAGT ACGAGAAGGC CCAGTCGTCC
    CAAGATGCTG TGTCTTCCAT GAACCTCTTT GACCTGGGTG GCCAGTACTT GCGGGTGGGC
    AAGGCTGTCA CACCGCCCAT GCCCCTACTC ACACCAGCCA CGCCTGGAGG CCTCCCACCT
    GCCGCTGCTG TGGCAGCTGC TGCAGCCACT GCCAAGATCA CAGCTCAGGA AGCAGTGGCC
    GGAGCAGCGG TGCTGGGTAC CCTGGGCACA CCTGGACTGG TGTCCCCAGC ACTGACCCTG
    GCCCAGCCCC TGGGCACTTT GCCCCAGGCT GTCATGGCTG CCCAGGCACC TGGAGTCATC
    ACAGGTGTGA CCCCAGCCCG TCCTCCTATC CCGGTCACCA TCCCCTCGGT GGGAGTGGTG
    AACCCCATCC TGGCCAGCCC TCCAACGCTG GGTCTCCTGG AGCCCAAGAA GGAGAAGGAA
    GAAGAGGAGC TGTTTCCCGA GTCAGAGCGG CCAGAGATGC TGAGCGAGCA GGAGCACATG
    AGCATCTCGG GCAGTAGCGC CCGACACATG GTGATGCAGA AGCTGCTCCG CAAGCAGGAG
    TCTACAGTGA TGGTTCTGCG CAACATGGTG GACCCCAAGG ACATCGATGA TGACCTGGAA
    GGGGAGGTGA CAGAGGAGTG TGGCAAGTTC GGGGCCGTGA ACCGCGTCAT CATCTACCAA
    GAGAAACAAG GCGAGGAGGA GGATGCAGAA ATCATTGTCA AGATCTTTGT GGAGTTTTCC
    ATAGCCTCTG AGACTCATAA GGCCATCCAG GCCCTCAATG GCCGCTGGTT TGCTGGCCGC
    AAGGTGGTGG CTGAAGTGTA CGACCAGGAG CGTTTTGATA ACAGTGACCT CTCTGCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu20314
    Clone ID Related Accession (Same CDS sequence) NM_078480.2 , NM_078480.3
    Accession Version NM_078480.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1680bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-22
    Organism Homo sapiens(human)
    Product poly(U)-binding-splicing factor PUF60 isoform a
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CN371576.1, AF114818.1, AF217197.2 and AW249619.1. This sequence is a reference standard in the RefSeqGene project. On Sep 1, 2012 this sequence version replaced NM_078480.1. Transcript Variant: This variant (1) encodes the isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF114818.1, SRR1803613.125708.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGGCGACGG CGACCATAGC TCTCCAGGTC AATGGCCAGC AAGGAGGGGG GTCCGAGCCG 
    GCGGCGGCGG CGGCAGTGGT GGCAGCGGGA GACAAATGGA AACCTCCACA GGGCACAGAC
    TCCATCAAGA TGGAGAACGG GCAGAGCACA GCCGCCAAGC TGGGGCTGCC TCCCCTGACG
    CCCGAGCAGC AGGAGGCCCT TCAGAAGGCC AAGAAGTACG CCATGGAGCA GAGCATCAAG
    AGTGTGCTGG TGAAGCAGAC CATCGCGCAC CAGCAGCAGC AGCTCACCAA CCTGCAGATG
    GCAGCAGTGA CAATGGGCTT TGGAGATCCT CTCTCACCTT TGCAATCGAT GGCGGCTCAG
    CGGCAGCGGG CGCTGGCCAT CATGTGCCGC GTCTACGTGG GCTCTATCTA CTATGAGCTG
    GGGGAGGACA CCATCCGCCA GGCCTTTGCC CCCTTTGGCC CCATCAAGAG CATCGACATG
    TCCTGGGACT CCGTCACCAT GAAGCACAAG GGCTTTGCCT TCGTGGAGTA TGAGGTCCCC
    GAAGCTGCAC AGCTGGCCTT GGAGCAGATG AACTCGGTGA TGCTGGGGGG CAGGAACATC
    AAGGTGGGCA GACCCAGCAA CATAGGGCAG GCCCAGCCCA TCATAGACCA GTTGGCTGAG
    GAGGCACGGG CCTTCAACCG CATCTACGTG GCCTCTGTGC ACCAGGACCT CTCAGACGAT
    GACATCAAGA GCGTGTTTGA GGCCTTTGGC AAGATCAAGT CCTGCACACT GGCCCGGGAC
    CCCACAACTG GCAAGCACAA GGGCTACGGC TTCATTGAGT ACGAGAAGGC CCAGTCGTCC
    CAAGATGCTG TGTCTTCCAT GAACCTCTTT GACCTGGGTG GCCAGTACTT GCGGGTGGGC
    AAGGCTGTCA CACCGCCCAT GCCCCTACTC ACACCAGCCA CGCCTGGAGG CCTCCCACCT
    GCCGCTGCTG TGGCAGCTGC TGCAGCCACT GCCAAGATCA CAGCTCAGGA AGCAGTGGCC
    GGAGCAGCGG TGCTGGGTAC CCTGGGCACA CCTGGACTGG TGTCCCCAGC ACTGACCCTG
    GCCCAGCCCC TGGGCACTTT GCCCCAGGCT GTCATGGCTG CCCAGGCACC TGGAGTCATC
    ACAGGTGTGA CCCCAGCCCG TCCTCCTATC CCGGTCACCA TCCCCTCGGT GGGAGTGGTG
    AACCCCATCC TGGCCAGCCC TCCAACGCTG GGTCTCCTGG AGCCCAAGAA GGAGAAGGAA
    GAAGAGGAGC TGTTTCCCGA GTCAGAGCGG CCAGAGATGC TGAGCGAGCA GGAGCACATG
    AGCATCTCGG GCAGTAGCGC CCGACACATG GTGATGCAGA AGCTGCTCCG CAAGCAGGAG
    TCTACAGTGA TGGTTCTGCG CAACATGGTG GACCCCAAGG ACATCGATGA TGACCTGGAA
    GGGGAGGTGA CAGAGGAGTG TGGCAAGTTC GGGGCCGTGA ACCGCGTCAT CATCTACCAA
    GAGAAACAAG GCGAGGAGGA GGATGCAGAA ATCATTGTCA AGATCTTTGT GGAGTTTTCC
    ATAGCCTCTG AGACTCATAA GGCCATCCAG GCCCTCAATG GCCGCTGGTT TGCTGGCCGC
    AAGGTGGTGG CTGAAGTGTA CGACCAGGAG CGTTTTGATA ACAGTGACCT CTCTGCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_510965.1
    CDS84..1763
    Translation

    Target ORF information:

    RefSeq Version NM_078480.2
    Organism Homo sapiens(human)
    Definition Homo sapiens poly(U) binding splicing factor 60 (PUF60), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_078480.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGGCGACGG CGACCATAGC TCTCCAGGTC AATGGCCAGC AAGGAGGGGG GTCCGAGCCG 
    GCGGCGGCGG CGGCAGTGGT GGCAGCGGGA GACAAATGGA AACCTCCACA GGGCACAGAC
    TCCATCAAGA TGGAGAACGG GCAGAGCACA GCCGCCAAGC TGGGGCTGCC TCCCCTGACG
    CCCGAGCAGC AGGAGGCCCT TCAGAAGGCC AAGAAGTACG CCATGGAGCA GAGCATCAAG
    AGTGTGCTGG TGAAGCAGAC CATCGCGCAC CAGCAGCAGC AGCTCACCAA CCTGCAGATG
    GCAGCAGTGA CAATGGGCTT TGGAGATCCT CTCTCACCTT TGCAATCGAT GGCGGCTCAG
    CGGCAGCGGG CGCTGGCCAT CATGTGCCGC GTCTACGTGG GCTCTATCTA CTATGAGCTG
    GGGGAGGACA CCATCCGCCA GGCCTTTGCC CCCTTTGGCC CCATCAAGAG CATCGACATG
    TCCTGGGACT CCGTCACCAT GAAGCACAAG GGCTTTGCCT TCGTGGAGTA TGAGGTCCCC
    GAAGCTGCAC AGCTGGCCTT GGAGCAGATG AACTCGGTGA TGCTGGGGGG CAGGAACATC
    AAGGTGGGCA GACCCAGCAA CATAGGGCAG GCCCAGCCCA TCATAGACCA GTTGGCTGAG
    GAGGCACGGG CCTTCAACCG CATCTACGTG GCCTCTGTGC ACCAGGACCT CTCAGACGAT
    GACATCAAGA GCGTGTTTGA GGCCTTTGGC AAGATCAAGT CCTGCACACT GGCCCGGGAC
    CCCACAACTG GCAAGCACAA GGGCTACGGC TTCATTGAGT ACGAGAAGGC CCAGTCGTCC
    CAAGATGCTG TGTCTTCCAT GAACCTCTTT GACCTGGGTG GCCAGTACTT GCGGGTGGGC
    AAGGCTGTCA CACCGCCCAT GCCCCTACTC ACACCAGCCA CGCCTGGAGG CCTCCCACCT
    GCCGCTGCTG TGGCAGCTGC TGCAGCCACT GCCAAGATCA CAGCTCAGGA AGCAGTGGCC
    GGAGCAGCGG TGCTGGGTAC CCTGGGCACA CCTGGACTGG TGTCCCCAGC ACTGACCCTG
    GCCCAGCCCC TGGGCACTTT GCCCCAGGCT GTCATGGCTG CCCAGGCACC TGGAGTCATC
    ACAGGTGTGA CCCCAGCCCG TCCTCCTATC CCGGTCACCA TCCCCTCGGT GGGAGTGGTG
    AACCCCATCC TGGCCAGCCC TCCAACGCTG GGTCTCCTGG AGCCCAAGAA GGAGAAGGAA
    GAAGAGGAGC TGTTTCCCGA GTCAGAGCGG CCAGAGATGC TGAGCGAGCA GGAGCACATG
    AGCATCTCGG GCAGTAGCGC CCGACACATG GTGATGCAGA AGCTGCTCCG CAAGCAGGAG
    TCTACAGTGA TGGTTCTGCG CAACATGGTG GACCCCAAGG ACATCGATGA TGACCTGGAA
    GGGGAGGTGA CAGAGGAGTG TGGCAAGTTC GGGGCCGTGA ACCGCGTCAT CATCTACCAA
    GAGAAACAAG GCGAGGAGGA GGATGCAGAA ATCATTGTCA AGATCTTTGT GGAGTTTTCC
    ATAGCCTCTG AGACTCATAA GGCCATCCAG GCCCTCAATG GCCGCTGGTT TGCTGGCCGC
    AAGGTGGTGG CTGAAGTGTA CGACCAGGAG CGTTTTGATA ACAGTGACCT CTCTGCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu20314
    Clone ID Related Accession (Same CDS sequence) NM_078480.2 , NM_078480.3
    Accession Version NM_078480.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1680bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-24
    Organism Homo sapiens(human)
    Product poly(U)-binding-splicing factor PUF60 isoform a
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF114818.1, AF217197.2 and AW249619.1. This sequence is a reference standard in the RefSeqGene project. On May 17, 2019 this sequence version replaced NM_078480.2. Transcript Variant: This variant (1) encodes the isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.13392.1, SRR1803611.197650.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000526683.6/ ENSP00000434359.1 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGGCGACGG CGACCATAGC TCTCCAGGTC AATGGCCAGC AAGGAGGGGG GTCCGAGCCG 
    GCGGCGGCGG CGGCAGTGGT GGCAGCGGGA GACAAATGGA AACCTCCACA GGGCACAGAC
    TCCATCAAGA TGGAGAACGG GCAGAGCACA GCCGCCAAGC TGGGGCTGCC TCCCCTGACG
    CCCGAGCAGC AGGAGGCCCT TCAGAAGGCC AAGAAGTACG CCATGGAGCA GAGCATCAAG
    AGTGTGCTGG TGAAGCAGAC CATCGCGCAC CAGCAGCAGC AGCTCACCAA CCTGCAGATG
    GCAGCAGTGA CAATGGGCTT TGGAGATCCT CTCTCACCTT TGCAATCGAT GGCGGCTCAG
    CGGCAGCGGG CGCTGGCCAT CATGTGCCGC GTCTACGTGG GCTCTATCTA CTATGAGCTG
    GGGGAGGACA CCATCCGCCA GGCCTTTGCC CCCTTTGGCC CCATCAAGAG CATCGACATG
    TCCTGGGACT CCGTCACCAT GAAGCACAAG GGCTTTGCCT TCGTGGAGTA TGAGGTCCCC
    GAAGCTGCAC AGCTGGCCTT GGAGCAGATG AACTCGGTGA TGCTGGGGGG CAGGAACATC
    AAGGTGGGCA GACCCAGCAA CATAGGGCAG GCCCAGCCCA TCATAGACCA GTTGGCTGAG
    GAGGCACGGG CCTTCAACCG CATCTACGTG GCCTCTGTGC ACCAGGACCT CTCAGACGAT
    GACATCAAGA GCGTGTTTGA GGCCTTTGGC AAGATCAAGT CCTGCACACT GGCCCGGGAC
    CCCACAACTG GCAAGCACAA GGGCTACGGC TTCATTGAGT ACGAGAAGGC CCAGTCGTCC
    CAAGATGCTG TGTCTTCCAT GAACCTCTTT GACCTGGGTG GCCAGTACTT GCGGGTGGGC
    AAGGCTGTCA CACCGCCCAT GCCCCTACTC ACACCAGCCA CGCCTGGAGG CCTCCCACCT
    GCCGCTGCTG TGGCAGCTGC TGCAGCCACT GCCAAGATCA CAGCTCAGGA AGCAGTGGCC
    GGAGCAGCGG TGCTGGGTAC CCTGGGCACA CCTGGACTGG TGTCCCCAGC ACTGACCCTG
    GCCCAGCCCC TGGGCACTTT GCCCCAGGCT GTCATGGCTG CCCAGGCACC TGGAGTCATC
    ACAGGTGTGA CCCCAGCCCG TCCTCCTATC CCGGTCACCA TCCCCTCGGT GGGAGTGGTG
    AACCCCATCC TGGCCAGCCC TCCAACGCTG GGTCTCCTGG AGCCCAAGAA GGAGAAGGAA
    GAAGAGGAGC TGTTTCCCGA GTCAGAGCGG CCAGAGATGC TGAGCGAGCA GGAGCACATG
    AGCATCTCGG GCAGTAGCGC CCGACACATG GTGATGCAGA AGCTGCTCCG CAAGCAGGAG
    TCTACAGTGA TGGTTCTGCG CAACATGGTG GACCCCAAGG ACATCGATGA TGACCTGGAA
    GGGGAGGTGA CAGAGGAGTG TGGCAAGTTC GGGGCCGTGA ACCGCGTCAT CATCTACCAA
    GAGAAACAAG GCGAGGAGGA GGATGCAGAA ATCATTGTCA AGATCTTTGT GGAGTTTTCC
    ATAGCCTCTG AGACTCATAA GGCCATCCAG GCCCTCAATG GCCGCTGGTT TGCTGGCCGC
    AAGGTGGTGG CTGAAGTGTA CGACCAGGAG CGTTTTGATA ACAGTGACCT CTCTGCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_510965.1
    CDS13..1692
    Translation

    Target ORF information:

    RefSeq Version NM_078480.3
    Organism Homo sapiens(human)
    Definition Homo sapiens poly(U) binding splicing factor 60 (PUF60), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_078480.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGGCGACGG CGACCATAGC TCTCCAGGTC AATGGCCAGC AAGGAGGGGG GTCCGAGCCG 
    GCGGCGGCGG CGGCAGTGGT GGCAGCGGGA GACAAATGGA AACCTCCACA GGGCACAGAC
    TCCATCAAGA TGGAGAACGG GCAGAGCACA GCCGCCAAGC TGGGGCTGCC TCCCCTGACG
    CCCGAGCAGC AGGAGGCCCT TCAGAAGGCC AAGAAGTACG CCATGGAGCA GAGCATCAAG
    AGTGTGCTGG TGAAGCAGAC CATCGCGCAC CAGCAGCAGC AGCTCACCAA CCTGCAGATG
    GCAGCAGTGA CAATGGGCTT TGGAGATCCT CTCTCACCTT TGCAATCGAT GGCGGCTCAG
    CGGCAGCGGG CGCTGGCCAT CATGTGCCGC GTCTACGTGG GCTCTATCTA CTATGAGCTG
    GGGGAGGACA CCATCCGCCA GGCCTTTGCC CCCTTTGGCC CCATCAAGAG CATCGACATG
    TCCTGGGACT CCGTCACCAT GAAGCACAAG GGCTTTGCCT TCGTGGAGTA TGAGGTCCCC
    GAAGCTGCAC AGCTGGCCTT GGAGCAGATG AACTCGGTGA TGCTGGGGGG CAGGAACATC
    AAGGTGGGCA GACCCAGCAA CATAGGGCAG GCCCAGCCCA TCATAGACCA GTTGGCTGAG
    GAGGCACGGG CCTTCAACCG CATCTACGTG GCCTCTGTGC ACCAGGACCT CTCAGACGAT
    GACATCAAGA GCGTGTTTGA GGCCTTTGGC AAGATCAAGT CCTGCACACT GGCCCGGGAC
    CCCACAACTG GCAAGCACAA GGGCTACGGC TTCATTGAGT ACGAGAAGGC CCAGTCGTCC
    CAAGATGCTG TGTCTTCCAT GAACCTCTTT GACCTGGGTG GCCAGTACTT GCGGGTGGGC
    AAGGCTGTCA CACCGCCCAT GCCCCTACTC ACACCAGCCA CGCCTGGAGG CCTCCCACCT
    GCCGCTGCTG TGGCAGCTGC TGCAGCCACT GCCAAGATCA CAGCTCAGGA AGCAGTGGCC
    GGAGCAGCGG TGCTGGGTAC CCTGGGCACA CCTGGACTGG TGTCCCCAGC ACTGACCCTG
    GCCCAGCCCC TGGGCACTTT GCCCCAGGCT GTCATGGCTG CCCAGGCACC TGGAGTCATC
    ACAGGTGTGA CCCCAGCCCG TCCTCCTATC CCGGTCACCA TCCCCTCGGT GGGAGTGGTG
    AACCCCATCC TGGCCAGCCC TCCAACGCTG GGTCTCCTGG AGCCCAAGAA GGAGAAGGAA
    GAAGAGGAGC TGTTTCCCGA GTCAGAGCGG CCAGAGATGC TGAGCGAGCA GGAGCACATG
    AGCATCTCGG GCAGTAGCGC CCGACACATG GTGATGCAGA AGCTGCTCCG CAAGCAGGAG
    TCTACAGTGA TGGTTCTGCG CAACATGGTG GACCCCAAGG ACATCGATGA TGACCTGGAA
    GGGGAGGTGA CAGAGGAGTG TGGCAAGTTC GGGGCCGTGA ACCGCGTCAT CATCTACCAA
    GAGAAACAAG GCGAGGAGGA GGATGCAGAA ATCATTGTCA AGATCTTTGT GGAGTTTTCC
    ATAGCCTCTG AGACTCATAA GGCCATCCAG GCCCTCAATG GCCGCTGGTT TGCTGGCCGC
    AAGGTGGTGG CTGAAGTGTA CGACCAGGAG CGTTTTGATA ACAGTGACCT CTCTGCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu20744
    Clone ID Related Accession (Same CDS sequence) NM_001271096.2 , NM_001271096.1
    Accession Version NM_001271096.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1626bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-27
    Organism Homo sapiens(human)
    Product poly(U)-binding-splicing factor PUF60 isoform d
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BX418925.2, AC105219.6, AF217197.2 and AW249619.1. On May 31, 2019 this sequence version replaced NM_001271096.1. Transcript Variant: This variant (4) uses an alternate in-frame splice site and lacks an alternate exon in the coding region, compared to variant 1, but maintains the reading frame. The encoded isoform (d) is shorter than isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.273076.1, SRR1803616.186532.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGGCGACGG CGACCATAGC TCTCGTCAAT GGCCAGCAAG GAGGGGGGTC CGAGCCGGCG 
    GCGGCGGCGG CAGTGGTGGC AGCGGGAGAC AAATGGAAAC CTCCACAGGG CACAGACTCC
    ATCAAGATGG AGAACGGGCA GAGCACAGCC GCCAAGCTGG GGCTGCCTCC CCTGACGCCC
    GAGCAGCAGG AGGCCCTTCA GAAGGCCAAG AAGTACGCCA TGGAGCAGAG CATCAAGAGT
    GTGCTGGTGA AGCAGACCAT CGCGCACCAG CAGCAGCAGC TCACCAACCT GCAGATGGCG
    GCTCAGCGGC AGCGGGCGCT GGCCATCATG TGCCGCGTCT ACGTGGGCTC TATCTACTAT
    GAGCTGGGGG AGGACACCAT CCGCCAGGCC TTTGCCCCCT TTGGCCCCAT CAAGAGCATC
    GACATGTCCT GGGACTCCGT CACCATGAAG CACAAGGGCT TTGCCTTCGT GGAGTATGAG
    GTCCCCGAAG CTGCACAGCT GGCCTTGGAG CAGATGAACT CGGTGATGCT GGGGGGCAGG
    AACATCAAGG TGGGCAGACC CAGCAACATA GGGCAGGCCC AGCCCATCAT AGACCAGTTG
    GCTGAGGAGG CACGGGCCTT CAACCGCATC TACGTGGCCT CTGTGCACCA GGACCTCTCA
    GACGATGACA TCAAGAGCGT GTTTGAGGCC TTTGGCAAGA TCAAGTCCTG CACACTGGCC
    CGGGACCCCA CAACTGGCAA GCACAAGGGC TACGGCTTCA TTGAGTACGA GAAGGCCCAG
    TCGTCCCAAG ATGCTGTGTC TTCCATGAAC CTCTTTGACC TGGGTGGCCA GTACTTGCGG
    GTGGGCAAGG CTGTCACACC GCCCATGCCC CTACTCACAC CAGCCACGCC TGGAGGCCTC
    CCACCTGCCG CTGCTGTGGC AGCTGCTGCA GCCACTGCCA AGATCACAGC TCAGGAAGCA
    GTGGCCGGAG CAGCGGTGCT GGGTACCCTG GGCACACCTG GACTGGTGTC CCCAGCACTG
    ACCCTGGCCC AGCCCCTGGG CACTTTGCCC CAGGCTGTCA TGGCTGCCCA GGCACCTGGA
    GTCATCACAG GTGTGACCCC AGCCCGTCCT CCTATCCCGG TCACCATCCC CTCGGTGGGA
    GTGGTGAACC CCATCCTGGC CAGCCCTCCA ACGCTGGGTC TCCTGGAGCC CAAGAAGGAG
    AAGGAAGAAG AGGAGCTGTT TCCCGAGTCA GAGCGGCCAG AGATGCTGAG CGAGCAGGAG
    CACATGAGCA TCTCGGGCAG TAGCGCCCGA CACATGGTGA TGCAGAAGCT GCTCCGCAAG
    CAGGAGTCTA CAGTGATGGT TCTGCGCAAC ATGGTGGACC CCAAGGACAT CGATGATGAC
    CTGGAAGGGG AGGTGACAGA GGAGTGTGGC AAGTTCGGGG CCGTGAACCG CGTCATCATC
    TACCAAGAGA AACAAGGCGA GGAGGAGGAT GCAGAAATCA TTGTCAAGAT CTTTGTGGAG
    TTTTCCATAG CCTCTGAGAC TCATAAGGCC ATCCAGGCCC TCAATGGCCG CTGGTTTGCT
    GGCCGCAAGG TGGTGGCTGA AGTGTACGAC CAGGAGCGTT TTGATAACAG TGACCTCTCT
    GCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001258025.1
    CDS13..1638
    Translation

    Target ORF information:

    RefSeq Version NM_001271096.2
    Organism Homo sapiens(human)
    Definition Homo sapiens poly(U) binding splicing factor 60 (PUF60), transcript variant 4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001271096.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGGCGACGG CGACCATAGC TCTCGTCAAT GGCCAGCAAG GAGGGGGGTC CGAGCCGGCG 
    GCGGCGGCGG CAGTGGTGGC AGCGGGAGAC AAATGGAAAC CTCCACAGGG CACAGACTCC
    ATCAAGATGG AGAACGGGCA GAGCACAGCC GCCAAGCTGG GGCTGCCTCC CCTGACGCCC
    GAGCAGCAGG AGGCCCTTCA GAAGGCCAAG AAGTACGCCA TGGAGCAGAG CATCAAGAGT
    GTGCTGGTGA AGCAGACCAT CGCGCACCAG CAGCAGCAGC TCACCAACCT GCAGATGGCG
    GCTCAGCGGC AGCGGGCGCT GGCCATCATG TGCCGCGTCT ACGTGGGCTC TATCTACTAT
    GAGCTGGGGG AGGACACCAT CCGCCAGGCC TTTGCCCCCT TTGGCCCCAT CAAGAGCATC
    GACATGTCCT GGGACTCCGT CACCATGAAG CACAAGGGCT TTGCCTTCGT GGAGTATGAG
    GTCCCCGAAG CTGCACAGCT GGCCTTGGAG CAGATGAACT CGGTGATGCT GGGGGGCAGG
    AACATCAAGG TGGGCAGACC CAGCAACATA GGGCAGGCCC AGCCCATCAT AGACCAGTTG
    GCTGAGGAGG CACGGGCCTT CAACCGCATC TACGTGGCCT CTGTGCACCA GGACCTCTCA
    GACGATGACA TCAAGAGCGT GTTTGAGGCC TTTGGCAAGA TCAAGTCCTG CACACTGGCC
    CGGGACCCCA CAACTGGCAA GCACAAGGGC TACGGCTTCA TTGAGTACGA GAAGGCCCAG
    TCGTCCCAAG ATGCTGTGTC TTCCATGAAC CTCTTTGACC TGGGTGGCCA GTACTTGCGG
    GTGGGCAAGG CTGTCACACC GCCCATGCCC CTACTCACAC CAGCCACGCC TGGAGGCCTC
    CCACCTGCCG CTGCTGTGGC AGCTGCTGCA GCCACTGCCA AGATCACAGC TCAGGAAGCA
    GTGGCCGGAG CAGCGGTGCT GGGTACCCTG GGCACACCTG GACTGGTGTC CCCAGCACTG
    ACCCTGGCCC AGCCCCTGGG CACTTTGCCC CAGGCTGTCA TGGCTGCCCA GGCACCTGGA
    GTCATCACAG GTGTGACCCC AGCCCGTCCT CCTATCCCGG TCACCATCCC CTCGGTGGGA
    GTGGTGAACC CCATCCTGGC CAGCCCTCCA ACGCTGGGTC TCCTGGAGCC CAAGAAGGAG
    AAGGAAGAAG AGGAGCTGTT TCCCGAGTCA GAGCGGCCAG AGATGCTGAG CGAGCAGGAG
    CACATGAGCA TCTCGGGCAG TAGCGCCCGA CACATGGTGA TGCAGAAGCT GCTCCGCAAG
    CAGGAGTCTA CAGTGATGGT TCTGCGCAAC ATGGTGGACC CCAAGGACAT CGATGATGAC
    CTGGAAGGGG AGGTGACAGA GGAGTGTGGC AAGTTCGGGG CCGTGAACCG CGTCATCATC
    TACCAAGAGA AACAAGGCGA GGAGGAGGAT GCAGAAATCA TTGTCAAGAT CTTTGTGGAG
    TTTTCCATAG CCTCTGAGAC TCATAAGGCC ATCCAGGCCC TCAATGGCCG CTGGTTTGCT
    GGCCGCAAGG TGGTGGCTGA AGTGTACGAC CAGGAGCGTT TTGATAACAG TGACCTCTCT
    GCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu20744
    Clone ID Related Accession (Same CDS sequence) NM_001271096.2 , NM_001271096.1
    Accession Version NM_001271096.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1626bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-12-03
    Organism Homo sapiens(human)
    Product poly(U)-binding-splicing factor PUF60 isoform d
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CN371576.1, BX418925.2, AC105219.6, AF217197.2 and AW249619.1. Transcript Variant: This variant (4) uses an alternate in-frame splice site and lacks an alternate exon in the coding region, compared to variant 1, but maintains the reading frame. The encoded isoform (d) is shorter than isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR3476690.421056.1, SRR3476690.810305.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGGCGACGG CGACCATAGC TCTCGTCAAT GGCCAGCAAG GAGGGGGGTC CGAGCCGGCG 
    GCGGCGGCGG CAGTGGTGGC AGCGGGAGAC AAATGGAAAC CTCCACAGGG CACAGACTCC
    ATCAAGATGG AGAACGGGCA GAGCACAGCC GCCAAGCTGG GGCTGCCTCC CCTGACGCCC
    GAGCAGCAGG AGGCCCTTCA GAAGGCCAAG AAGTACGCCA TGGAGCAGAG CATCAAGAGT
    GTGCTGGTGA AGCAGACCAT CGCGCACCAG CAGCAGCAGC TCACCAACCT GCAGATGGCG
    GCTCAGCGGC AGCGGGCGCT GGCCATCATG TGCCGCGTCT ACGTGGGCTC TATCTACTAT
    GAGCTGGGGG AGGACACCAT CCGCCAGGCC TTTGCCCCCT TTGGCCCCAT CAAGAGCATC
    GACATGTCCT GGGACTCCGT CACCATGAAG CACAAGGGCT TTGCCTTCGT GGAGTATGAG
    GTCCCCGAAG CTGCACAGCT GGCCTTGGAG CAGATGAACT CGGTGATGCT GGGGGGCAGG
    AACATCAAGG TGGGCAGACC CAGCAACATA GGGCAGGCCC AGCCCATCAT AGACCAGTTG
    GCTGAGGAGG CACGGGCCTT CAACCGCATC TACGTGGCCT CTGTGCACCA GGACCTCTCA
    GACGATGACA TCAAGAGCGT GTTTGAGGCC TTTGGCAAGA TCAAGTCCTG CACACTGGCC
    CGGGACCCCA CAACTGGCAA GCACAAGGGC TACGGCTTCA TTGAGTACGA GAAGGCCCAG
    TCGTCCCAAG ATGCTGTGTC TTCCATGAAC CTCTTTGACC TGGGTGGCCA GTACTTGCGG
    GTGGGCAAGG CTGTCACACC GCCCATGCCC CTACTCACAC CAGCCACGCC TGGAGGCCTC
    CCACCTGCCG CTGCTGTGGC AGCTGCTGCA GCCACTGCCA AGATCACAGC TCAGGAAGCA
    GTGGCCGGAG CAGCGGTGCT GGGTACCCTG GGCACACCTG GACTGGTGTC CCCAGCACTG
    ACCCTGGCCC AGCCCCTGGG CACTTTGCCC CAGGCTGTCA TGGCTGCCCA GGCACCTGGA
    GTCATCACAG GTGTGACCCC AGCCCGTCCT CCTATCCCGG TCACCATCCC CTCGGTGGGA
    GTGGTGAACC CCATCCTGGC CAGCCCTCCA ACGCTGGGTC TCCTGGAGCC CAAGAAGGAG
    AAGGAAGAAG AGGAGCTGTT TCCCGAGTCA GAGCGGCCAG AGATGCTGAG CGAGCAGGAG
    CACATGAGCA TCTCGGGCAG TAGCGCCCGA CACATGGTGA TGCAGAAGCT GCTCCGCAAG
    CAGGAGTCTA CAGTGATGGT TCTGCGCAAC ATGGTGGACC CCAAGGACAT CGATGATGAC
    CTGGAAGGGG AGGTGACAGA GGAGTGTGGC AAGTTCGGGG CCGTGAACCG CGTCATCATC
    TACCAAGAGA AACAAGGCGA GGAGGAGGAT GCAGAAATCA TTGTCAAGAT CTTTGTGGAG
    TTTTCCATAG CCTCTGAGAC TCATAAGGCC ATCCAGGCCC TCAATGGCCG CTGGTTTGCT
    GGCCGCAAGG TGGTGGCTGA AGTGTACGAC CAGGAGCGTT TTGATAACAG TGACCTCTCT
    GCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001258025.1
    CDS84..1709
    Translation

    Target ORF information:

    RefSeq Version NM_001271096.1
    Organism Homo sapiens(human)
    Definition Homo sapiens poly(U) binding splicing factor 60 (PUF60), transcript variant 4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001271096.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGGCGACGG CGACCATAGC TCTCGTCAAT GGCCAGCAAG GAGGGGGGTC CGAGCCGGCG 
    GCGGCGGCGG CAGTGGTGGC AGCGGGAGAC AAATGGAAAC CTCCACAGGG CACAGACTCC
    ATCAAGATGG AGAACGGGCA GAGCACAGCC GCCAAGCTGG GGCTGCCTCC CCTGACGCCC
    GAGCAGCAGG AGGCCCTTCA GAAGGCCAAG AAGTACGCCA TGGAGCAGAG CATCAAGAGT
    GTGCTGGTGA AGCAGACCAT CGCGCACCAG CAGCAGCAGC TCACCAACCT GCAGATGGCG
    GCTCAGCGGC AGCGGGCGCT GGCCATCATG TGCCGCGTCT ACGTGGGCTC TATCTACTAT
    GAGCTGGGGG AGGACACCAT CCGCCAGGCC TTTGCCCCCT TTGGCCCCAT CAAGAGCATC
    GACATGTCCT GGGACTCCGT CACCATGAAG CACAAGGGCT TTGCCTTCGT GGAGTATGAG
    GTCCCCGAAG CTGCACAGCT GGCCTTGGAG CAGATGAACT CGGTGATGCT GGGGGGCAGG
    AACATCAAGG TGGGCAGACC CAGCAACATA GGGCAGGCCC AGCCCATCAT AGACCAGTTG
    GCTGAGGAGG CACGGGCCTT CAACCGCATC TACGTGGCCT CTGTGCACCA GGACCTCTCA
    GACGATGACA TCAAGAGCGT GTTTGAGGCC TTTGGCAAGA TCAAGTCCTG CACACTGGCC
    CGGGACCCCA CAACTGGCAA GCACAAGGGC TACGGCTTCA TTGAGTACGA GAAGGCCCAG
    TCGTCCCAAG ATGCTGTGTC TTCCATGAAC CTCTTTGACC TGGGTGGCCA GTACTTGCGG
    GTGGGCAAGG CTGTCACACC GCCCATGCCC CTACTCACAC CAGCCACGCC TGGAGGCCTC
    CCACCTGCCG CTGCTGTGGC AGCTGCTGCA GCCACTGCCA AGATCACAGC TCAGGAAGCA
    GTGGCCGGAG CAGCGGTGCT GGGTACCCTG GGCACACCTG GACTGGTGTC CCCAGCACTG
    ACCCTGGCCC AGCCCCTGGG CACTTTGCCC CAGGCTGTCA TGGCTGCCCA GGCACCTGGA
    GTCATCACAG GTGTGACCCC AGCCCGTCCT CCTATCCCGG TCACCATCCC CTCGGTGGGA
    GTGGTGAACC CCATCCTGGC CAGCCCTCCA ACGCTGGGTC TCCTGGAGCC CAAGAAGGAG
    AAGGAAGAAG AGGAGCTGTT TCCCGAGTCA GAGCGGCCAG AGATGCTGAG CGAGCAGGAG
    CACATGAGCA TCTCGGGCAG TAGCGCCCGA CACATGGTGA TGCAGAAGCT GCTCCGCAAG
    CAGGAGTCTA CAGTGATGGT TCTGCGCAAC ATGGTGGACC CCAAGGACAT CGATGATGAC
    CTGGAAGGGG AGGTGACAGA GGAGTGTGGC AAGTTCGGGG CCGTGAACCG CGTCATCATC
    TACCAAGAGA AACAAGGCGA GGAGGAGGAT GCAGAAATCA TTGTCAAGAT CTTTGTGGAG
    TTTTCCATAG CCTCTGAGAC TCATAAGGCC ATCCAGGCCC TCAATGGCCG CTGGTTTGCT
    GGCCGCAAGG TGGTGGCTGA AGTGTACGAC CAGGAGCGTT TTGATAACAG TGACCTCTCT
    GCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu21541
    Clone ID Related Accession (Same CDS sequence) NM_001271098.2 , NM_001271098.1
    Accession Version NM_001271098.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1677bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-27
    Organism Homo sapiens(human)
    Product poly(U)-binding-splicing factor PUF60 isoform f
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL522753.3, AC105219.6, AF217197.2 and AW249619.1. On May 31, 2019 this sequence version replaced NM_001271098.1. Transcript Variant: This variant (6) uses an alternate in-frame splice site in the coding region, compared to variant 1. The encoded isoform (f) is shorter than isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.15736.1, SRR1803615.184871.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGGCGACGG CGACCATAGC TCTCGTCAAT GGCCAGCAAG GAGGGGGGTC CGAGCCGGCG 
    GCGGCGGCGG CAGTGGTGGC AGCGGGAGAC AAATGGAAAC CTCCACAGGG CACAGACTCC
    ATCAAGATGG AGAACGGGCA GAGCACAGCC GCCAAGCTGG GGCTGCCTCC CCTGACGCCC
    GAGCAGCAGG AGGCCCTTCA GAAGGCCAAG AAGTACGCCA TGGAGCAGAG CATCAAGAGT
    GTGCTGGTGA AGCAGACCAT CGCGCACCAG CAGCAGCAGC TCACCAACCT GCAGATGGCA
    GCAGTGACAA TGGGCTTTGG AGATCCTCTC TCACCTTTGC AATCGATGGC GGCTCAGCGG
    CAGCGGGCGC TGGCCATCAT GTGCCGCGTC TACGTGGGCT CTATCTACTA TGAGCTGGGG
    GAGGACACCA TCCGCCAGGC CTTTGCCCCC TTTGGCCCCA TCAAGAGCAT CGACATGTCC
    TGGGACTCCG TCACCATGAA GCACAAGGGC TTTGCCTTCG TGGAGTATGA GGTCCCCGAA
    GCTGCACAGC TGGCCTTGGA GCAGATGAAC TCGGTGATGC TGGGGGGCAG GAACATCAAG
    GTGGGCAGAC CCAGCAACAT AGGGCAGGCC CAGCCCATCA TAGACCAGTT GGCTGAGGAG
    GCACGGGCCT TCAACCGCAT CTACGTGGCC TCTGTGCACC AGGACCTCTC AGACGATGAC
    ATCAAGAGCG TGTTTGAGGC CTTTGGCAAG ATCAAGTCCT GCACACTGGC CCGGGACCCC
    ACAACTGGCA AGCACAAGGG CTACGGCTTC ATTGAGTACG AGAAGGCCCA GTCGTCCCAA
    GATGCTGTGT CTTCCATGAA CCTCTTTGAC CTGGGTGGCC AGTACTTGCG GGTGGGCAAG
    GCTGTCACAC CGCCCATGCC CCTACTCACA CCAGCCACGC CTGGAGGCCT CCCACCTGCC
    GCTGCTGTGG CAGCTGCTGC AGCCACTGCC AAGATCACAG CTCAGGAAGC AGTGGCCGGA
    GCAGCGGTGC TGGGTACCCT GGGCACACCT GGACTGGTGT CCCCAGCACT GACCCTGGCC
    CAGCCCCTGG GCACTTTGCC CCAGGCTGTC ATGGCTGCCC AGGCACCTGG AGTCATCACA
    GGTGTGACCC CAGCCCGTCC TCCTATCCCG GTCACCATCC CCTCGGTGGG AGTGGTGAAC
    CCCATCCTGG CCAGCCCTCC AACGCTGGGT CTCCTGGAGC CCAAGAAGGA GAAGGAAGAA
    GAGGAGCTGT TTCCCGAGTC AGAGCGGCCA GAGATGCTGA GCGAGCAGGA GCACATGAGC
    ATCTCGGGCA GTAGCGCCCG ACACATGGTG ATGCAGAAGC TGCTCCGCAA GCAGGAGTCT
    ACAGTGATGG TTCTGCGCAA CATGGTGGAC CCCAAGGACA TCGATGATGA CCTGGAAGGG
    GAGGTGACAG AGGAGTGTGG CAAGTTCGGG GCCGTGAACC GCGTCATCAT CTACCAAGAG
    AAACAAGGCG AGGAGGAGGA TGCAGAAATC ATTGTCAAGA TCTTTGTGGA GTTTTCCATA
    GCCTCTGAGA CTCATAAGGC CATCCAGGCC CTCAATGGCC GCTGGTTTGC TGGCCGCAAG
    GTGGTGGCTG AAGTGTACGA CCAGGAGCGT TTTGATAACA GTGACCTCTC TGCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001258027.1
    CDS13..1689
    Translation

    Target ORF information:

    RefSeq Version NM_001271098.2
    Organism Homo sapiens(human)
    Definition Homo sapiens poly(U) binding splicing factor 60 (PUF60), transcript variant 6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001271098.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGGCGACGG CGACCATAGC TCTCGTCAAT GGCCAGCAAG GAGGGGGGTC CGAGCCGGCG 
    GCGGCGGCGG CAGTGGTGGC AGCGGGAGAC AAATGGAAAC CTCCACAGGG CACAGACTCC
    ATCAAGATGG AGAACGGGCA GAGCACAGCC GCCAAGCTGG GGCTGCCTCC CCTGACGCCC
    GAGCAGCAGG AGGCCCTTCA GAAGGCCAAG AAGTACGCCA TGGAGCAGAG CATCAAGAGT
    GTGCTGGTGA AGCAGACCAT CGCGCACCAG CAGCAGCAGC TCACCAACCT GCAGATGGCA
    GCAGTGACAA TGGGCTTTGG AGATCCTCTC TCACCTTTGC AATCGATGGC GGCTCAGCGG
    CAGCGGGCGC TGGCCATCAT GTGCCGCGTC TACGTGGGCT CTATCTACTA TGAGCTGGGG
    GAGGACACCA TCCGCCAGGC CTTTGCCCCC TTTGGCCCCA TCAAGAGCAT CGACATGTCC
    TGGGACTCCG TCACCATGAA GCACAAGGGC TTTGCCTTCG TGGAGTATGA GGTCCCCGAA
    GCTGCACAGC TGGCCTTGGA GCAGATGAAC TCGGTGATGC TGGGGGGCAG GAACATCAAG
    GTGGGCAGAC CCAGCAACAT AGGGCAGGCC CAGCCCATCA TAGACCAGTT GGCTGAGGAG
    GCACGGGCCT TCAACCGCAT CTACGTGGCC TCTGTGCACC AGGACCTCTC AGACGATGAC
    ATCAAGAGCG TGTTTGAGGC CTTTGGCAAG ATCAAGTCCT GCACACTGGC CCGGGACCCC
    ACAACTGGCA AGCACAAGGG CTACGGCTTC ATTGAGTACG AGAAGGCCCA GTCGTCCCAA
    GATGCTGTGT CTTCCATGAA CCTCTTTGAC CTGGGTGGCC AGTACTTGCG GGTGGGCAAG
    GCTGTCACAC CGCCCATGCC CCTACTCACA CCAGCCACGC CTGGAGGCCT CCCACCTGCC
    GCTGCTGTGG CAGCTGCTGC AGCCACTGCC AAGATCACAG CTCAGGAAGC AGTGGCCGGA
    GCAGCGGTGC TGGGTACCCT GGGCACACCT GGACTGGTGT CCCCAGCACT GACCCTGGCC
    CAGCCCCTGG GCACTTTGCC CCAGGCTGTC ATGGCTGCCC AGGCACCTGG AGTCATCACA
    GGTGTGACCC CAGCCCGTCC TCCTATCCCG GTCACCATCC CCTCGGTGGG AGTGGTGAAC
    CCCATCCTGG CCAGCCCTCC AACGCTGGGT CTCCTGGAGC CCAAGAAGGA GAAGGAAGAA
    GAGGAGCTGT TTCCCGAGTC AGAGCGGCCA GAGATGCTGA GCGAGCAGGA GCACATGAGC
    ATCTCGGGCA GTAGCGCCCG ACACATGGTG ATGCAGAAGC TGCTCCGCAA GCAGGAGTCT
    ACAGTGATGG TTCTGCGCAA CATGGTGGAC CCCAAGGACA TCGATGATGA CCTGGAAGGG
    GAGGTGACAG AGGAGTGTGG CAAGTTCGGG GCCGTGAACC GCGTCATCAT CTACCAAGAG
    AAACAAGGCG AGGAGGAGGA TGCAGAAATC ATTGTCAAGA TCTTTGTGGA GTTTTCCATA
    GCCTCTGAGA CTCATAAGGC CATCCAGGCC CTCAATGGCC GCTGGTTTGC TGGCCGCAAG
    GTGGTGGCTG AAGTGTACGA CCAGGAGCGT TTTGATAACA GTGACCTCTC TGCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu21541
    Clone ID Related Accession (Same CDS sequence) NM_001271098.2 , NM_001271098.1
    Accession Version NM_001271098.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1677bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-22
    Organism Homo sapiens(human)
    Product poly(U)-binding-splicing factor PUF60 isoform f
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CN371576.1, AL522753.3, AC105219.6, AF217197.2 and AW249619.1. Transcript Variant: This variant (6) uses an alternate in-frame splice site in the coding region, compared to variant 1. The encoded isoform (f) is shorter than isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR3476690.217690.1, SRR3476690.52274.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGGCGACGG CGACCATAGC TCTCGTCAAT GGCCAGCAAG GAGGGGGGTC CGAGCCGGCG 
    GCGGCGGCGG CAGTGGTGGC AGCGGGAGAC AAATGGAAAC CTCCACAGGG CACAGACTCC
    ATCAAGATGG AGAACGGGCA GAGCACAGCC GCCAAGCTGG GGCTGCCTCC CCTGACGCCC
    GAGCAGCAGG AGGCCCTTCA GAAGGCCAAG AAGTACGCCA TGGAGCAGAG CATCAAGAGT
    GTGCTGGTGA AGCAGACCAT CGCGCACCAG CAGCAGCAGC TCACCAACCT GCAGATGGCA
    GCAGTGACAA TGGGCTTTGG AGATCCTCTC TCACCTTTGC AATCGATGGC GGCTCAGCGG
    CAGCGGGCGC TGGCCATCAT GTGCCGCGTC TACGTGGGCT CTATCTACTA TGAGCTGGGG
    GAGGACACCA TCCGCCAGGC CTTTGCCCCC TTTGGCCCCA TCAAGAGCAT CGACATGTCC
    TGGGACTCCG TCACCATGAA GCACAAGGGC TTTGCCTTCG TGGAGTATGA GGTCCCCGAA
    GCTGCACAGC TGGCCTTGGA GCAGATGAAC TCGGTGATGC TGGGGGGCAG GAACATCAAG
    GTGGGCAGAC CCAGCAACAT AGGGCAGGCC CAGCCCATCA TAGACCAGTT GGCTGAGGAG
    GCACGGGCCT TCAACCGCAT CTACGTGGCC TCTGTGCACC AGGACCTCTC AGACGATGAC
    ATCAAGAGCG TGTTTGAGGC CTTTGGCAAG ATCAAGTCCT GCACACTGGC CCGGGACCCC
    ACAACTGGCA AGCACAAGGG CTACGGCTTC ATTGAGTACG AGAAGGCCCA GTCGTCCCAA
    GATGCTGTGT CTTCCATGAA CCTCTTTGAC CTGGGTGGCC AGTACTTGCG GGTGGGCAAG
    GCTGTCACAC CGCCCATGCC CCTACTCACA CCAGCCACGC CTGGAGGCCT CCCACCTGCC
    GCTGCTGTGG CAGCTGCTGC AGCCACTGCC AAGATCACAG CTCAGGAAGC AGTGGCCGGA
    GCAGCGGTGC TGGGTACCCT GGGCACACCT GGACTGGTGT CCCCAGCACT GACCCTGGCC
    CAGCCCCTGG GCACTTTGCC CCAGGCTGTC ATGGCTGCCC AGGCACCTGG AGTCATCACA
    GGTGTGACCC CAGCCCGTCC TCCTATCCCG GTCACCATCC CCTCGGTGGG AGTGGTGAAC
    CCCATCCTGG CCAGCCCTCC AACGCTGGGT CTCCTGGAGC CCAAGAAGGA GAAGGAAGAA
    GAGGAGCTGT TTCCCGAGTC AGAGCGGCCA GAGATGCTGA GCGAGCAGGA GCACATGAGC
    ATCTCGGGCA GTAGCGCCCG ACACATGGTG ATGCAGAAGC TGCTCCGCAA GCAGGAGTCT
    ACAGTGATGG TTCTGCGCAA CATGGTGGAC CCCAAGGACA TCGATGATGA CCTGGAAGGG
    GAGGTGACAG AGGAGTGTGG CAAGTTCGGG GCCGTGAACC GCGTCATCAT CTACCAAGAG
    AAACAAGGCG AGGAGGAGGA TGCAGAAATC ATTGTCAAGA TCTTTGTGGA GTTTTCCATA
    GCCTCTGAGA CTCATAAGGC CATCCAGGCC CTCAATGGCC GCTGGTTTGC TGGCCGCAAG
    GTGGTGGCTG AAGTGTACGA CCAGGAGCGT TTTGATAACA GTGACCTCTC TGCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001258027.1
    CDS84..1760
    Translation

    Target ORF information:

    RefSeq Version NM_001271098.1
    Organism Homo sapiens(human)
    Definition Homo sapiens poly(U) binding splicing factor 60 (PUF60), transcript variant 6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001271098.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGGCGACGG CGACCATAGC TCTCGTCAAT GGCCAGCAAG GAGGGGGGTC CGAGCCGGCG 
    GCGGCGGCGG CAGTGGTGGC AGCGGGAGAC AAATGGAAAC CTCCACAGGG CACAGACTCC
    ATCAAGATGG AGAACGGGCA GAGCACAGCC GCCAAGCTGG GGCTGCCTCC CCTGACGCCC
    GAGCAGCAGG AGGCCCTTCA GAAGGCCAAG AAGTACGCCA TGGAGCAGAG CATCAAGAGT
    GTGCTGGTGA AGCAGACCAT CGCGCACCAG CAGCAGCAGC TCACCAACCT GCAGATGGCA
    GCAGTGACAA TGGGCTTTGG AGATCCTCTC TCACCTTTGC AATCGATGGC GGCTCAGCGG
    CAGCGGGCGC TGGCCATCAT GTGCCGCGTC TACGTGGGCT CTATCTACTA TGAGCTGGGG
    GAGGACACCA TCCGCCAGGC CTTTGCCCCC TTTGGCCCCA TCAAGAGCAT CGACATGTCC
    TGGGACTCCG TCACCATGAA GCACAAGGGC TTTGCCTTCG TGGAGTATGA GGTCCCCGAA
    GCTGCACAGC TGGCCTTGGA GCAGATGAAC TCGGTGATGC TGGGGGGCAG GAACATCAAG
    GTGGGCAGAC CCAGCAACAT AGGGCAGGCC CAGCCCATCA TAGACCAGTT GGCTGAGGAG
    GCACGGGCCT TCAACCGCAT CTACGTGGCC TCTGTGCACC AGGACCTCTC AGACGATGAC
    ATCAAGAGCG TGTTTGAGGC CTTTGGCAAG ATCAAGTCCT GCACACTGGC CCGGGACCCC
    ACAACTGGCA AGCACAAGGG CTACGGCTTC ATTGAGTACG AGAAGGCCCA GTCGTCCCAA
    GATGCTGTGT CTTCCATGAA CCTCTTTGAC CTGGGTGGCC AGTACTTGCG GGTGGGCAAG
    GCTGTCACAC CGCCCATGCC CCTACTCACA CCAGCCACGC CTGGAGGCCT CCCACCTGCC
    GCTGCTGTGG CAGCTGCTGC AGCCACTGCC AAGATCACAG CTCAGGAAGC AGTGGCCGGA
    GCAGCGGTGC TGGGTACCCT GGGCACACCT GGACTGGTGT CCCCAGCACT GACCCTGGCC
    CAGCCCCTGG GCACTTTGCC CCAGGCTGTC ATGGCTGCCC AGGCACCTGG AGTCATCACA
    GGTGTGACCC CAGCCCGTCC TCCTATCCCG GTCACCATCC CCTCGGTGGG AGTGGTGAAC
    CCCATCCTGG CCAGCCCTCC AACGCTGGGT CTCCTGGAGC CCAAGAAGGA GAAGGAAGAA
    GAGGAGCTGT TTCCCGAGTC AGAGCGGCCA GAGATGCTGA GCGAGCAGGA GCACATGAGC
    ATCTCGGGCA GTAGCGCCCG ACACATGGTG ATGCAGAAGC TGCTCCGCAA GCAGGAGTCT
    ACAGTGATGG TTCTGCGCAA CATGGTGGAC CCCAAGGACA TCGATGATGA CCTGGAAGGG
    GAGGTGACAG AGGAGTGTGG CAAGTTCGGG GCCGTGAACC GCGTCATCAT CTACCAAGAG
    AAACAAGGCG AGGAGGAGGA TGCAGAAATC ATTGTCAAGA TCTTTGTGGA GTTTTCCATA
    GCCTCTGAGA CTCATAAGGC CATCCAGGCC CTCAATGGCC GCTGGTTTGC TGGCCGCAAG
    GTGGTGGCTG AAGTGTACGA CCAGGAGCGT TTTGATAACA GTGACCTCTC TGCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu27468
    Clone ID Related Accession (Same CDS sequence) NM_001271099.2 , NM_001271099.1
    Accession Version NM_001271099.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1593bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-27
    Organism Homo sapiens(human)
    Product poly(U)-binding-splicing factor PUF60 isoform g
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CT000277.1, AF217197.2 and AW249619.1. On May 31, 2019 this sequence version replaced NM_001271099.1. Transcript Variant: This variant (7, also known as PUF60deltaexon2) lacks an alternate in-frame exon in the coding region, compared to variant 1. The encoded isoform (g) is shorter than isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.17120.1, SRR1660809.127209.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGGCGACGG CGACCATAGC TCTCGGCACA GACTCCATCA AGATGGAGAA CGGGCAGAGC 
    ACAGCCGCCA AGCTGGGGCT GCCTCCCCTG ACGCCCGAGC AGCAGGAGGC CCTTCAGAAG
    GCCAAGAAGT ACGCCATGGA GCAGAGCATC AAGAGTGTGC TGGTGAAGCA GACCATCGCG
    CACCAGCAGC AGCAGCTCAC CAACCTGCAG ATGGCAGCAG TGACAATGGG CTTTGGAGAT
    CCTCTCTCAC CTTTGCAATC GATGGCGGCT CAGCGGCAGC GGGCGCTGGC CATCATGTGC
    CGCGTCTACG TGGGCTCTAT CTACTATGAG CTGGGGGAGG ACACCATCCG CCAGGCCTTT
    GCCCCCTTTG GCCCCATCAA GAGCATCGAC ATGTCCTGGG ACTCCGTCAC CATGAAGCAC
    AAGGGCTTTG CCTTCGTGGA GTATGAGGTC CCCGAAGCTG CACAGCTGGC CTTGGAGCAG
    ATGAACTCGG TGATGCTGGG GGGCAGGAAC ATCAAGGTGG GCAGACCCAG CAACATAGGG
    CAGGCCCAGC CCATCATAGA CCAGTTGGCT GAGGAGGCAC GGGCCTTCAA CCGCATCTAC
    GTGGCCTCTG TGCACCAGGA CCTCTCAGAC GATGACATCA AGAGCGTGTT TGAGGCCTTT
    GGCAAGATCA AGTCCTGCAC ACTGGCCCGG GACCCCACAA CTGGCAAGCA CAAGGGCTAC
    GGCTTCATTG AGTACGAGAA GGCCCAGTCG TCCCAAGATG CTGTGTCTTC CATGAACCTC
    TTTGACCTGG GTGGCCAGTA CTTGCGGGTG GGCAAGGCTG TCACACCGCC CATGCCCCTA
    CTCACACCAG CCACGCCTGG AGGCCTCCCA CCTGCCGCTG CTGTGGCAGC TGCTGCAGCC
    ACTGCCAAGA TCACAGCTCA GGAAGCAGTG GCCGGAGCAG CGGTGCTGGG TACCCTGGGC
    ACACCTGGAC TGGTGTCCCC AGCACTGACC CTGGCCCAGC CCCTGGGCAC TTTGCCCCAG
    GCTGTCATGG CTGCCCAGGC ACCTGGAGTC ATCACAGGTG TGACCCCAGC CCGTCCTCCT
    ATCCCGGTCA CCATCCCCTC GGTGGGAGTG GTGAACCCCA TCCTGGCCAG CCCTCCAACG
    CTGGGTCTCC TGGAGCCCAA GAAGGAGAAG GAAGAAGAGG AGCTGTTTCC CGAGTCAGAG
    CGGCCAGAGA TGCTGAGCGA GCAGGAGCAC ATGAGCATCT CGGGCAGTAG CGCCCGACAC
    ATGGTGATGC AGAAGCTGCT CCGCAAGCAG GAGTCTACAG TGATGGTTCT GCGCAACATG
    GTGGACCCCA AGGACATCGA TGATGACCTG GAAGGGGAGG TGACAGAGGA GTGTGGCAAG
    TTCGGGGCCG TGAACCGCGT CATCATCTAC CAAGAGAAAC AAGGCGAGGA GGAGGATGCA
    GAAATCATTG TCAAGATCTT TGTGGAGTTT TCCATAGCCT CTGAGACTCA TAAGGCCATC
    CAGGCCCTCA ATGGCCGCTG GTTTGCTGGC CGCAAGGTGG TGGCTGAAGT GTACGACCAG
    GAGCGTTTTG ATAACAGTGA CCTCTCTGCG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001258028.1
    CDS13..1605
    Translation

    Target ORF information:

    RefSeq Version NM_001271099.2
    Organism Homo sapiens(human)
    Definition Homo sapiens poly(U) binding splicing factor 60 (PUF60), transcript variant 7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001271099.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGGCGACGG CGACCATAGC TCTCGGCACA GACTCCATCA AGATGGAGAA CGGGCAGAGC 
    ACAGCCGCCA AGCTGGGGCT GCCTCCCCTG ACGCCCGAGC AGCAGGAGGC CCTTCAGAAG
    GCCAAGAAGT ACGCCATGGA GCAGAGCATC AAGAGTGTGC TGGTGAAGCA GACCATCGCG
    CACCAGCAGC AGCAGCTCAC CAACCTGCAG ATGGCAGCAG TGACAATGGG CTTTGGAGAT
    CCTCTCTCAC CTTTGCAATC GATGGCGGCT CAGCGGCAGC GGGCGCTGGC CATCATGTGC
    CGCGTCTACG TGGGCTCTAT CTACTATGAG CTGGGGGAGG ACACCATCCG CCAGGCCTTT
    GCCCCCTTTG GCCCCATCAA GAGCATCGAC ATGTCCTGGG ACTCCGTCAC CATGAAGCAC
    AAGGGCTTTG CCTTCGTGGA GTATGAGGTC CCCGAAGCTG CACAGCTGGC CTTGGAGCAG
    ATGAACTCGG TGATGCTGGG GGGCAGGAAC ATCAAGGTGG GCAGACCCAG CAACATAGGG
    CAGGCCCAGC CCATCATAGA CCAGTTGGCT GAGGAGGCAC GGGCCTTCAA CCGCATCTAC
    GTGGCCTCTG TGCACCAGGA CCTCTCAGAC GATGACATCA AGAGCGTGTT TGAGGCCTTT
    GGCAAGATCA AGTCCTGCAC ACTGGCCCGG GACCCCACAA CTGGCAAGCA CAAGGGCTAC
    GGCTTCATTG AGTACGAGAA GGCCCAGTCG TCCCAAGATG CTGTGTCTTC CATGAACCTC
    TTTGACCTGG GTGGCCAGTA CTTGCGGGTG GGCAAGGCTG TCACACCGCC CATGCCCCTA
    CTCACACCAG CCACGCCTGG AGGCCTCCCA CCTGCCGCTG CTGTGGCAGC TGCTGCAGCC
    ACTGCCAAGA TCACAGCTCA GGAAGCAGTG GCCGGAGCAG CGGTGCTGGG TACCCTGGGC
    ACACCTGGAC TGGTGTCCCC AGCACTGACC CTGGCCCAGC CCCTGGGCAC TTTGCCCCAG
    GCTGTCATGG CTGCCCAGGC ACCTGGAGTC ATCACAGGTG TGACCCCAGC CCGTCCTCCT
    ATCCCGGTCA CCATCCCCTC GGTGGGAGTG GTGAACCCCA TCCTGGCCAG CCCTCCAACG
    CTGGGTCTCC TGGAGCCCAA GAAGGAGAAG GAAGAAGAGG AGCTGTTTCC CGAGTCAGAG
    CGGCCAGAGA TGCTGAGCGA GCAGGAGCAC ATGAGCATCT CGGGCAGTAG CGCCCGACAC
    ATGGTGATGC AGAAGCTGCT CCGCAAGCAG GAGTCTACAG TGATGGTTCT GCGCAACATG
    GTGGACCCCA AGGACATCGA TGATGACCTG GAAGGGGAGG TGACAGAGGA GTGTGGCAAG
    TTCGGGGCCG TGAACCGCGT CATCATCTAC CAAGAGAAAC AAGGCGAGGA GGAGGATGCA
    GAAATCATTG TCAAGATCTT TGTGGAGTTT TCCATAGCCT CTGAGACTCA TAAGGCCATC
    CAGGCCCTCA ATGGCCGCTG GTTTGCTGGC CGCAAGGTGG TGGCTGAAGT GTACGACCAG
    GAGCGTTTTG ATAACAGTGA CCTCTCTGCG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu27468
    Clone ID Related Accession (Same CDS sequence) NM_001271099.2 , NM_001271099.1
    Accession Version NM_001271099.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1593bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-12-03
    Organism Homo sapiens(human)
    Product poly(U)-binding-splicing factor PUF60 isoform g
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CN371576.1, CT000277.1, AF217197.2 and AW249619.1. Transcript Variant: This variant (7, also known as PUF60deltaexon2) lacks an alternate in-frame exon in the coding region, compared to variant 1. The encoded isoform (g) is shorter than isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR3476690.187996.1, SRR3476690.1147880.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGGCGACGG CGACCATAGC TCTCGGCACA GACTCCATCA AGATGGAGAA CGGGCAGAGC 
    ACAGCCGCCA AGCTGGGGCT GCCTCCCCTG ACGCCCGAGC AGCAGGAGGC CCTTCAGAAG
    GCCAAGAAGT ACGCCATGGA GCAGAGCATC AAGAGTGTGC TGGTGAAGCA GACCATCGCG
    CACCAGCAGC AGCAGCTCAC CAACCTGCAG ATGGCAGCAG TGACAATGGG CTTTGGAGAT
    CCTCTCTCAC CTTTGCAATC GATGGCGGCT CAGCGGCAGC GGGCGCTGGC CATCATGTGC
    CGCGTCTACG TGGGCTCTAT CTACTATGAG CTGGGGGAGG ACACCATCCG CCAGGCCTTT
    GCCCCCTTTG GCCCCATCAA GAGCATCGAC ATGTCCTGGG ACTCCGTCAC CATGAAGCAC
    AAGGGCTTTG CCTTCGTGGA GTATGAGGTC CCCGAAGCTG CACAGCTGGC CTTGGAGCAG
    ATGAACTCGG TGATGCTGGG GGGCAGGAAC ATCAAGGTGG GCAGACCCAG CAACATAGGG
    CAGGCCCAGC CCATCATAGA CCAGTTGGCT GAGGAGGCAC GGGCCTTCAA CCGCATCTAC
    GTGGCCTCTG TGCACCAGGA CCTCTCAGAC GATGACATCA AGAGCGTGTT TGAGGCCTTT
    GGCAAGATCA AGTCCTGCAC ACTGGCCCGG GACCCCACAA CTGGCAAGCA CAAGGGCTAC
    GGCTTCATTG AGTACGAGAA GGCCCAGTCG TCCCAAGATG CTGTGTCTTC CATGAACCTC
    TTTGACCTGG GTGGCCAGTA CTTGCGGGTG GGCAAGGCTG TCACACCGCC CATGCCCCTA
    CTCACACCAG CCACGCCTGG AGGCCTCCCA CCTGCCGCTG CTGTGGCAGC TGCTGCAGCC
    ACTGCCAAGA TCACAGCTCA GGAAGCAGTG GCCGGAGCAG CGGTGCTGGG TACCCTGGGC
    ACACCTGGAC TGGTGTCCCC AGCACTGACC CTGGCCCAGC CCCTGGGCAC TTTGCCCCAG
    GCTGTCATGG CTGCCCAGGC ACCTGGAGTC ATCACAGGTG TGACCCCAGC CCGTCCTCCT
    ATCCCGGTCA CCATCCCCTC GGTGGGAGTG GTGAACCCCA TCCTGGCCAG CCCTCCAACG
    CTGGGTCTCC TGGAGCCCAA GAAGGAGAAG GAAGAAGAGG AGCTGTTTCC CGAGTCAGAG
    CGGCCAGAGA TGCTGAGCGA GCAGGAGCAC ATGAGCATCT CGGGCAGTAG CGCCCGACAC
    ATGGTGATGC AGAAGCTGCT CCGCAAGCAG GAGTCTACAG TGATGGTTCT GCGCAACATG
    GTGGACCCCA AGGACATCGA TGATGACCTG GAAGGGGAGG TGACAGAGGA GTGTGGCAAG
    TTCGGGGCCG TGAACCGCGT CATCATCTAC CAAGAGAAAC AAGGCGAGGA GGAGGATGCA
    GAAATCATTG TCAAGATCTT TGTGGAGTTT TCCATAGCCT CTGAGACTCA TAAGGCCATC
    CAGGCCCTCA ATGGCCGCTG GTTTGCTGGC CGCAAGGTGG TGGCTGAAGT GTACGACCAG
    GAGCGTTTTG ATAACAGTGA CCTCTCTGCG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001258028.1
    CDS84..1676
    Translation

    Target ORF information:

    RefSeq Version NM_001271099.1
    Organism Homo sapiens(human)
    Definition Homo sapiens poly(U) binding splicing factor 60 (PUF60), transcript variant 7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001271099.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGGCGACGG CGACCATAGC TCTCGGCACA GACTCCATCA AGATGGAGAA CGGGCAGAGC 
    ACAGCCGCCA AGCTGGGGCT GCCTCCCCTG ACGCCCGAGC AGCAGGAGGC CCTTCAGAAG
    GCCAAGAAGT ACGCCATGGA GCAGAGCATC AAGAGTGTGC TGGTGAAGCA GACCATCGCG
    CACCAGCAGC AGCAGCTCAC CAACCTGCAG ATGGCAGCAG TGACAATGGG CTTTGGAGAT
    CCTCTCTCAC CTTTGCAATC GATGGCGGCT CAGCGGCAGC GGGCGCTGGC CATCATGTGC
    CGCGTCTACG TGGGCTCTAT CTACTATGAG CTGGGGGAGG ACACCATCCG CCAGGCCTTT
    GCCCCCTTTG GCCCCATCAA GAGCATCGAC ATGTCCTGGG ACTCCGTCAC CATGAAGCAC
    AAGGGCTTTG CCTTCGTGGA GTATGAGGTC CCCGAAGCTG CACAGCTGGC CTTGGAGCAG
    ATGAACTCGG TGATGCTGGG GGGCAGGAAC ATCAAGGTGG GCAGACCCAG CAACATAGGG
    CAGGCCCAGC CCATCATAGA CCAGTTGGCT GAGGAGGCAC GGGCCTTCAA CCGCATCTAC
    GTGGCCTCTG TGCACCAGGA CCTCTCAGAC GATGACATCA AGAGCGTGTT TGAGGCCTTT
    GGCAAGATCA AGTCCTGCAC ACTGGCCCGG GACCCCACAA CTGGCAAGCA CAAGGGCTAC
    GGCTTCATTG AGTACGAGAA GGCCCAGTCG TCCCAAGATG CTGTGTCTTC CATGAACCTC
    TTTGACCTGG GTGGCCAGTA CTTGCGGGTG GGCAAGGCTG TCACACCGCC CATGCCCCTA
    CTCACACCAG CCACGCCTGG AGGCCTCCCA CCTGCCGCTG CTGTGGCAGC TGCTGCAGCC
    ACTGCCAAGA TCACAGCTCA GGAAGCAGTG GCCGGAGCAG CGGTGCTGGG TACCCTGGGC
    ACACCTGGAC TGGTGTCCCC AGCACTGACC CTGGCCCAGC CCCTGGGCAC TTTGCCCCAG
    GCTGTCATGG CTGCCCAGGC ACCTGGAGTC ATCACAGGTG TGACCCCAGC CCGTCCTCCT
    ATCCCGGTCA CCATCCCCTC GGTGGGAGTG GTGAACCCCA TCCTGGCCAG CCCTCCAACG
    CTGGGTCTCC TGGAGCCCAA GAAGGAGAAG GAAGAAGAGG AGCTGTTTCC CGAGTCAGAG
    CGGCCAGAGA TGCTGAGCGA GCAGGAGCAC ATGAGCATCT CGGGCAGTAG CGCCCGACAC
    ATGGTGATGC AGAAGCTGCT CCGCAAGCAG GAGTCTACAG TGATGGTTCT GCGCAACATG
    GTGGACCCCA AGGACATCGA TGATGACCTG GAAGGGGAGG TGACAGAGGA GTGTGGCAAG
    TTCGGGGCCG TGAACCGCGT CATCATCTAC CAAGAGAAAC AAGGCGAGGA GGAGGATGCA
    GAAATCATTG TCAAGATCTT TGTGGAGTTT TCCATAGCCT CTGAGACTCA TAAGGCCATC
    CAGGCCCTCA ATGGCCGCTG GTTTGCTGGC CGCAAGGTGG TGGCTGAAGT GTACGACCAG
    GAGCGTTTTG ATAACAGTGA CCTCTCTGCG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu27624
    Clone ID Related Accession (Same CDS sequence) NM_001271097.1 , NM_001271097.2
    Accession Version NM_001271097.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1542bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-12-03
    Organism Homo sapiens(human)
    Product poly(U)-binding-splicing factor PUF60 isoform e
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CN371576.1, CX163878.1, AF217197.2 and AW249619.1. Transcript Variant: This variant (5, also known as FIRdeltaexon2) lacks two alternate in-frame exons in the coding region, compared to variant 1. The encoded isoform (e) is shorter than isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR3476690.832280.1, SRR3476690.816463.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGGCGACGG CGACCATAGC TCTCGGCACA GACTCCATCA AGATGGAGAA CGGGCAGAGC 
    ACAGCCGCCA AGCTGGGGCT GCCTCCCCTG ACGCCCGAGC AGCAGGAGGC CCTTCAGAAG
    GCCAAGAAGT ACGCCATGGA GCAGAGCATC AAGAGTGTGC TGGTGAAGCA GACCATCGCG
    CACCAGCAGC AGCAGCTCAC CAACCTGCAG ATGGCGGCTC AGCGGCAGCG GGCGCTGGCC
    ATCATGTGCC GCGTCTACGT GGGCTCTATC TACTATGAGC TGGGGGAGGA CACCATCCGC
    CAGGCCTTTG CCCCCTTTGG CCCCATCAAG AGCATCGACA TGTCCTGGGA CTCCGTCACC
    ATGAAGCACA AGGGCTTTGC CTTCGTGGAG TATGAGGTCC CCGAAGCTGC ACAGCTGGCC
    TTGGAGCAGA TGAACTCGGT GATGCTGGGG GGCAGGAACA TCAAGGTGGG CAGACCCAGC
    AACATAGGGC AGGCCCAGCC CATCATAGAC CAGTTGGCTG AGGAGGCACG GGCCTTCAAC
    CGCATCTACG TGGCCTCTGT GCACCAGGAC CTCTCAGACG ATGACATCAA GAGCGTGTTT
    GAGGCCTTTG GCAAGATCAA GTCCTGCACA CTGGCCCGGG ACCCCACAAC TGGCAAGCAC
    AAGGGCTACG GCTTCATTGA GTACGAGAAG GCCCAGTCGT CCCAAGATGC TGTGTCTTCC
    ATGAACCTCT TTGACCTGGG TGGCCAGTAC TTGCGGGTGG GCAAGGCTGT CACACCGCCC
    ATGCCCCTAC TCACACCAGC CACGCCTGGA GGCCTCCCAC CTGCCGCTGC TGTGGCAGCT
    GCTGCAGCCA CTGCCAAGAT CACAGCTCAG GAAGCAGTGG CCGGAGCAGC GGTGCTGGGT
    ACCCTGGGCA CACCTGGACT GGTGTCCCCA GCACTGACCC TGGCCCAGCC CCTGGGCACT
    TTGCCCCAGG CTGTCATGGC TGCCCAGGCA CCTGGAGTCA TCACAGGTGT GACCCCAGCC
    CGTCCTCCTA TCCCGGTCAC CATCCCCTCG GTGGGAGTGG TGAACCCCAT CCTGGCCAGC
    CCTCCAACGC TGGGTCTCCT GGAGCCCAAG AAGGAGAAGG AAGAAGAGGA GCTGTTTCCC
    GAGTCAGAGC GGCCAGAGAT GCTGAGCGAG CAGGAGCACA TGAGCATCTC GGGCAGTAGC
    GCCCGACACA TGGTGATGCA GAAGCTGCTC CGCAAGCAGG AGTCTACAGT GATGGTTCTG
    CGCAACATGG TGGACCCCAA GGACATCGAT GATGACCTGG AAGGGGAGGT GACAGAGGAG
    TGTGGCAAGT TCGGGGCCGT GAACCGCGTC ATCATCTACC AAGAGAAACA AGGCGAGGAG
    GAGGATGCAG AAATCATTGT CAAGATCTTT GTGGAGTTTT CCATAGCCTC TGAGACTCAT
    AAGGCCATCC AGGCCCTCAA TGGCCGCTGG TTTGCTGGCC GCAAGGTGGT GGCTGAAGTG
    TACGACCAGG AGCGTTTTGA TAACAGTGAC CTCTCTGCGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001258026.1
    CDS84..1625
    Translation

    Target ORF information:

    RefSeq Version NM_001271097.1
    Organism Homo sapiens(human)
    Definition Homo sapiens poly(U) binding splicing factor 60 (PUF60), transcript variant 5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001271097.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGGCGACGG CGACCATAGC TCTCGGCACA GACTCCATCA AGATGGAGAA CGGGCAGAGC 
    ACAGCCGCCA AGCTGGGGCT GCCTCCCCTG ACGCCCGAGC AGCAGGAGGC CCTTCAGAAG
    GCCAAGAAGT ACGCCATGGA GCAGAGCATC AAGAGTGTGC TGGTGAAGCA GACCATCGCG
    CACCAGCAGC AGCAGCTCAC CAACCTGCAG ATGGCGGCTC AGCGGCAGCG GGCGCTGGCC
    ATCATGTGCC GCGTCTACGT GGGCTCTATC TACTATGAGC TGGGGGAGGA CACCATCCGC
    CAGGCCTTTG CCCCCTTTGG CCCCATCAAG AGCATCGACA TGTCCTGGGA CTCCGTCACC
    ATGAAGCACA AGGGCTTTGC CTTCGTGGAG TATGAGGTCC CCGAAGCTGC ACAGCTGGCC
    TTGGAGCAGA TGAACTCGGT GATGCTGGGG GGCAGGAACA TCAAGGTGGG CAGACCCAGC
    AACATAGGGC AGGCCCAGCC CATCATAGAC CAGTTGGCTG AGGAGGCACG GGCCTTCAAC
    CGCATCTACG TGGCCTCTGT GCACCAGGAC CTCTCAGACG ATGACATCAA GAGCGTGTTT
    GAGGCCTTTG GCAAGATCAA GTCCTGCACA CTGGCCCGGG ACCCCACAAC TGGCAAGCAC
    AAGGGCTACG GCTTCATTGA GTACGAGAAG GCCCAGTCGT CCCAAGATGC TGTGTCTTCC
    ATGAACCTCT TTGACCTGGG TGGCCAGTAC TTGCGGGTGG GCAAGGCTGT CACACCGCCC
    ATGCCCCTAC TCACACCAGC CACGCCTGGA GGCCTCCCAC CTGCCGCTGC TGTGGCAGCT
    GCTGCAGCCA CTGCCAAGAT CACAGCTCAG GAAGCAGTGG CCGGAGCAGC GGTGCTGGGT
    ACCCTGGGCA CACCTGGACT GGTGTCCCCA GCACTGACCC TGGCCCAGCC CCTGGGCACT
    TTGCCCCAGG CTGTCATGGC TGCCCAGGCA CCTGGAGTCA TCACAGGTGT GACCCCAGCC
    CGTCCTCCTA TCCCGGTCAC CATCCCCTCG GTGGGAGTGG TGAACCCCAT CCTGGCCAGC
    CCTCCAACGC TGGGTCTCCT GGAGCCCAAG AAGGAGAAGG AAGAAGAGGA GCTGTTTCCC
    GAGTCAGAGC GGCCAGAGAT GCTGAGCGAG CAGGAGCACA TGAGCATCTC GGGCAGTAGC
    GCCCGACACA TGGTGATGCA GAAGCTGCTC CGCAAGCAGG AGTCTACAGT GATGGTTCTG
    CGCAACATGG TGGACCCCAA GGACATCGAT GATGACCTGG AAGGGGAGGT GACAGAGGAG
    TGTGGCAAGT TCGGGGCCGT GAACCGCGTC ATCATCTACC AAGAGAAACA AGGCGAGGAG
    GAGGATGCAG AAATCATTGT CAAGATCTTT GTGGAGTTTT CCATAGCCTC TGAGACTCAT
    AAGGCCATCC AGGCCCTCAA TGGCCGCTGG TTTGCTGGCC GCAAGGTGGT GGCTGAAGTG
    TACGACCAGG AGCGTTTTGA TAACAGTGAC CTCTCTGCGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu27624
    Clone ID Related Accession (Same CDS sequence) NM_001271097.1 , NM_001271097.2
    Accession Version NM_001271097.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1542bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-27
    Organism Homo sapiens(human)
    Product poly(U)-binding-splicing factor PUF60 isoform e
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CX163878.1, AF217197.2 and AW249619.1. On May 31, 2019 this sequence version replaced NM_001271097.1. Transcript Variant: This variant (5, also known as FIRdeltaexon2) lacks two alternate in-frame exons in the coding region, compared to variant 1. The encoded isoform (e) is shorter than isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR5189661.113030.1, SRR5189655.101952.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGGCGACGG CGACCATAGC TCTCGGCACA GACTCCATCA AGATGGAGAA CGGGCAGAGC 
    ACAGCCGCCA AGCTGGGGCT GCCTCCCCTG ACGCCCGAGC AGCAGGAGGC CCTTCAGAAG
    GCCAAGAAGT ACGCCATGGA GCAGAGCATC AAGAGTGTGC TGGTGAAGCA GACCATCGCG
    CACCAGCAGC AGCAGCTCAC CAACCTGCAG ATGGCGGCTC AGCGGCAGCG GGCGCTGGCC
    ATCATGTGCC GCGTCTACGT GGGCTCTATC TACTATGAGC TGGGGGAGGA CACCATCCGC
    CAGGCCTTTG CCCCCTTTGG CCCCATCAAG AGCATCGACA TGTCCTGGGA CTCCGTCACC
    ATGAAGCACA AGGGCTTTGC CTTCGTGGAG TATGAGGTCC CCGAAGCTGC ACAGCTGGCC
    TTGGAGCAGA TGAACTCGGT GATGCTGGGG GGCAGGAACA TCAAGGTGGG CAGACCCAGC
    AACATAGGGC AGGCCCAGCC CATCATAGAC CAGTTGGCTG AGGAGGCACG GGCCTTCAAC
    CGCATCTACG TGGCCTCTGT GCACCAGGAC CTCTCAGACG ATGACATCAA GAGCGTGTTT
    GAGGCCTTTG GCAAGATCAA GTCCTGCACA CTGGCCCGGG ACCCCACAAC TGGCAAGCAC
    AAGGGCTACG GCTTCATTGA GTACGAGAAG GCCCAGTCGT CCCAAGATGC TGTGTCTTCC
    ATGAACCTCT TTGACCTGGG TGGCCAGTAC TTGCGGGTGG GCAAGGCTGT CACACCGCCC
    ATGCCCCTAC TCACACCAGC CACGCCTGGA GGCCTCCCAC CTGCCGCTGC TGTGGCAGCT
    GCTGCAGCCA CTGCCAAGAT CACAGCTCAG GAAGCAGTGG CCGGAGCAGC GGTGCTGGGT
    ACCCTGGGCA CACCTGGACT GGTGTCCCCA GCACTGACCC TGGCCCAGCC CCTGGGCACT
    TTGCCCCAGG CTGTCATGGC TGCCCAGGCA CCTGGAGTCA TCACAGGTGT GACCCCAGCC
    CGTCCTCCTA TCCCGGTCAC CATCCCCTCG GTGGGAGTGG TGAACCCCAT CCTGGCCAGC
    CCTCCAACGC TGGGTCTCCT GGAGCCCAAG AAGGAGAAGG AAGAAGAGGA GCTGTTTCCC
    GAGTCAGAGC GGCCAGAGAT GCTGAGCGAG CAGGAGCACA TGAGCATCTC GGGCAGTAGC
    GCCCGACACA TGGTGATGCA GAAGCTGCTC CGCAAGCAGG AGTCTACAGT GATGGTTCTG
    CGCAACATGG TGGACCCCAA GGACATCGAT GATGACCTGG AAGGGGAGGT GACAGAGGAG
    TGTGGCAAGT TCGGGGCCGT GAACCGCGTC ATCATCTACC AAGAGAAACA AGGCGAGGAG
    GAGGATGCAG AAATCATTGT CAAGATCTTT GTGGAGTTTT CCATAGCCTC TGAGACTCAT
    AAGGCCATCC AGGCCCTCAA TGGCCGCTGG TTTGCTGGCC GCAAGGTGGT GGCTGAAGTG
    TACGACCAGG AGCGTTTTGA TAACAGTGAC CTCTCTGCGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001258026.1
    CDS13..1554
    Translation

    Target ORF information:

    RefSeq Version NM_001271097.2
    Organism Homo sapiens(human)
    Definition Homo sapiens poly(U) binding splicing factor 60 (PUF60), transcript variant 5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001271097.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGGCGACGG CGACCATAGC TCTCGGCACA GACTCCATCA AGATGGAGAA CGGGCAGAGC 
    ACAGCCGCCA AGCTGGGGCT GCCTCCCCTG ACGCCCGAGC AGCAGGAGGC CCTTCAGAAG
    GCCAAGAAGT ACGCCATGGA GCAGAGCATC AAGAGTGTGC TGGTGAAGCA GACCATCGCG
    CACCAGCAGC AGCAGCTCAC CAACCTGCAG ATGGCGGCTC AGCGGCAGCG GGCGCTGGCC
    ATCATGTGCC GCGTCTACGT GGGCTCTATC TACTATGAGC TGGGGGAGGA CACCATCCGC
    CAGGCCTTTG CCCCCTTTGG CCCCATCAAG AGCATCGACA TGTCCTGGGA CTCCGTCACC
    ATGAAGCACA AGGGCTTTGC CTTCGTGGAG TATGAGGTCC CCGAAGCTGC ACAGCTGGCC
    TTGGAGCAGA TGAACTCGGT GATGCTGGGG GGCAGGAACA TCAAGGTGGG CAGACCCAGC
    AACATAGGGC AGGCCCAGCC CATCATAGAC CAGTTGGCTG AGGAGGCACG GGCCTTCAAC
    CGCATCTACG TGGCCTCTGT GCACCAGGAC CTCTCAGACG ATGACATCAA GAGCGTGTTT
    GAGGCCTTTG GCAAGATCAA GTCCTGCACA CTGGCCCGGG ACCCCACAAC TGGCAAGCAC
    AAGGGCTACG GCTTCATTGA GTACGAGAAG GCCCAGTCGT CCCAAGATGC TGTGTCTTCC
    ATGAACCTCT TTGACCTGGG TGGCCAGTAC TTGCGGGTGG GCAAGGCTGT CACACCGCCC
    ATGCCCCTAC TCACACCAGC CACGCCTGGA GGCCTCCCAC CTGCCGCTGC TGTGGCAGCT
    GCTGCAGCCA CTGCCAAGAT CACAGCTCAG GAAGCAGTGG CCGGAGCAGC GGTGCTGGGT
    ACCCTGGGCA CACCTGGACT GGTGTCCCCA GCACTGACCC TGGCCCAGCC CCTGGGCACT
    TTGCCCCAGG CTGTCATGGC TGCCCAGGCA CCTGGAGTCA TCACAGGTGT GACCCCAGCC
    CGTCCTCCTA TCCCGGTCAC CATCCCCTCG GTGGGAGTGG TGAACCCCAT CCTGGCCAGC
    CCTCCAACGC TGGGTCTCCT GGAGCCCAAG AAGGAGAAGG AAGAAGAGGA GCTGTTTCCC
    GAGTCAGAGC GGCCAGAGAT GCTGAGCGAG CAGGAGCACA TGAGCATCTC GGGCAGTAGC
    GCCCGACACA TGGTGATGCA GAAGCTGCTC CGCAAGCAGG AGTCTACAGT GATGGTTCTG
    CGCAACATGG TGGACCCCAA GGACATCGAT GATGACCTGG AAGGGGAGGT GACAGAGGAG
    TGTGGCAAGT TCGGGGCCGT GAACCGCGTC ATCATCTACC AAGAGAAACA AGGCGAGGAG
    GAGGATGCAG AAATCATTGT CAAGATCTTT GTGGAGTTTT CCATAGCCTC TGAGACTCAT
    AAGGCCATCC AGGCCCTCAA TGGCCGCTGG TTTGCTGGCC GCAAGGTGGT GGCTGAAGTG
    TACGACCAGG AGCGTTTTGA TAACAGTGAC CTCTCTGCGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu64963
    Clone ID Related Accession (Same CDS sequence) XM_011516929.2 , NM_001362896.2 , NM_001362895.1 , NM_001362896.1 , XM_017013238.1 , XM_017013237.1 , XM_017013234.1 , NM_001362895.2
    Accession Version NM_001362896.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1791bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-27
    Organism Homo sapiens(human)
    Product poly(U)-binding-splicing factor PUF60 isoform i
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BG771821.1, BC011265.1, AC105219.6 and AW249619.1. On May 31, 2019 this sequence version replaced NM_001362896.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.198393.1, SRR1660807.223171.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGGAGCAGC TGAACTTGGC TGACAGTGGC CTGGTGGGGA AGGAAGCCAG AAGATGCAGA 
    AGGGCCTCCC CTCCCATGGG CATGCCTTCT GCCTGGTGGC TGCAGAATGA GGCCGGCTGC
    CTCTTTGCAG CAAGGCAGGT CAATGGCCAG CAAGGAGGGG GGTCCGAGCC GGCGGCGGCG
    GCGGCAGTGG TGGCAGCGGG AGACAAATGG AAACCTCCAC AGGGCACAGA CTCCATCAAG
    ATGGAGAACG GGCAGAGCAC AGCCGCCAAG CTGGGGCTGC CTCCCCTGAC GCCCGAGCAG
    CAGGAGGCCC TTCAGAAGGC CAAGAAGTAC GCCATGGAGC AGAGCATCAA GAGTGTGCTG
    GTGAAGCAGA CCATCGCGCA CCAGCAGCAG CAGCTCACCA ACCTGCAGAT GGCAGCAGTG
    ACAATGGGCT TTGGAGATCC TCTCTCACCT TTGCAATCGA TGGCGGCTCA GCGGCAGCGG
    GCGCTGGCCA TCATGTGCCG CGTCTACGTG GGCTCTATCT ACTATGAGCT GGGGGAGGAC
    ACCATCCGCC AGGCCTTTGC CCCCTTTGGC CCCATCAAGA GCATCGACAT GTCCTGGGAC
    TCCGTCACCA TGAAGCACAA GGGCTTTGCC TTCGTGGAGT ATGAGGTCCC CGAAGCTGCA
    CAGCTGGCCT TGGAGCAGAT GAACTCGGTG ATGCTGGGGG GCAGGAACAT CAAGGTGGGC
    AGACCCAGCA ACATAGGGCA GGCCCAGCCC ATCATAGACC AGTTGGCTGA GGAGGCACGG
    GCCTTCAACC GCATCTACGT GGCCTCTGTG CACCAGGACC TCTCAGACGA TGACATCAAG
    AGCGTGTTTG AGGCCTTTGG CAAGATCAAG TCCTGCACAC TGGCCCGGGA CCCCACAACT
    GGCAAGCACA AGGGCTACGG CTTCATTGAG TACGAGAAGG CCCAGTCGTC CCAAGATGCT
    GTGTCTTCCA TGAACCTCTT TGACCTGGGT GGCCAGTACT TGCGGGTGGG CAAGGCTGTC
    ACACCGCCCA TGCCCCTACT CACACCAGCC ACGCCTGGAG GCCTCCCACC TGCCGCTGCT
    GTGGCAGCTG CTGCAGCCAC TGCCAAGATC ACAGCTCAGG AAGCAGTGGC CGGAGCAGCG
    GTGCTGGGTA CCCTGGGCAC ACCTGGACTG GTGTCCCCAG CACTGACCCT GGCCCAGCCC
    CTGGGCACTT TGCCCCAGGC TGTCATGGCT GCCCAGGCAC CTGGAGTCAT CACAGGTGTG
    ACCCCAGCCC GTCCTCCTAT CCCGGTCACC ATCCCCTCGG TGGGAGTGGT GAACCCCATC
    CTGGCCAGCC CTCCAACGCT GGGTCTCCTG GAGCCCAAGA AGGAGAAGGA AGAAGAGGAG
    CTGTTTCCCG AGTCAGAGCG GCCAGAGATG CTGAGCGAGC AGGAGCACAT GAGCATCTCG
    GGCAGTAGCG CCCGACACAT GGTGATGCAG AAGCTGCTCC GCAAGCAGGA GTCTACAGTG
    ATGGTTCTGC GCAACATGGT GGACCCCAAG GACATCGATG ATGACCTGGA AGGGGAGGTG
    ACAGAGGAGT GTGGCAAGTT CGGGGCCGTG AACCGCGTCA TCATCTACCA AGAGAAACAA
    GGCGAGGAGG AGGATGCAGA AATCATTGTC AAGATCTTTG TGGAGTTTTC CATAGCCTCT
    GAGACTCATA AGGCCATCCA GGCCCTCAAT GGCCGCTGGT TTGCTGGCCG CAAGGTGGTG
    GCTGAAGTGT ACGACCAGGA GCGTTTTGAT AACAGTGACC TCTCTGCGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001349825.1
    CDS239..2029
    Translation

    Target ORF information:

    RefSeq Version NM_001362896.2
    Organism Homo sapiens(human)
    Definition Homo sapiens poly(U) binding splicing factor 60 (PUF60), transcript variant 10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001362896.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGGAGCAGC TGAACTTGGC TGACAGTGGC CTGGTGGGGA AGGAAGCCAG AAGATGCAGA 
    AGGGCCTCCC CTCCCATGGG CATGCCTTCT GCCTGGTGGC TGCAGAATGA GGCCGGCTGC
    CTCTTTGCAG CAAGGCAGGT CAATGGCCAG CAAGGAGGGG GGTCCGAGCC GGCGGCGGCG
    GCGGCAGTGG TGGCAGCGGG AGACAAATGG AAACCTCCAC AGGGCACAGA CTCCATCAAG
    ATGGAGAACG GGCAGAGCAC AGCCGCCAAG CTGGGGCTGC CTCCCCTGAC GCCCGAGCAG
    CAGGAGGCCC TTCAGAAGGC CAAGAAGTAC GCCATGGAGC AGAGCATCAA GAGTGTGCTG
    GTGAAGCAGA CCATCGCGCA CCAGCAGCAG CAGCTCACCA ACCTGCAGAT GGCAGCAGTG
    ACAATGGGCT TTGGAGATCC TCTCTCACCT TTGCAATCGA TGGCGGCTCA GCGGCAGCGG
    GCGCTGGCCA TCATGTGCCG CGTCTACGTG GGCTCTATCT ACTATGAGCT GGGGGAGGAC
    ACCATCCGCC AGGCCTTTGC CCCCTTTGGC CCCATCAAGA GCATCGACAT GTCCTGGGAC
    TCCGTCACCA TGAAGCACAA GGGCTTTGCC TTCGTGGAGT ATGAGGTCCC CGAAGCTGCA
    CAGCTGGCCT TGGAGCAGAT GAACTCGGTG ATGCTGGGGG GCAGGAACAT CAAGGTGGGC
    AGACCCAGCA ACATAGGGCA GGCCCAGCCC ATCATAGACC AGTTGGCTGA GGAGGCACGG
    GCCTTCAACC GCATCTACGT GGCCTCTGTG CACCAGGACC TCTCAGACGA TGACATCAAG
    AGCGTGTTTG AGGCCTTTGG CAAGATCAAG TCCTGCACAC TGGCCCGGGA CCCCACAACT
    GGCAAGCACA AGGGCTACGG CTTCATTGAG TACGAGAAGG CCCAGTCGTC CCAAGATGCT
    GTGTCTTCCA TGAACCTCTT TGACCTGGGT GGCCAGTACT TGCGGGTGGG CAAGGCTGTC
    ACACCGCCCA TGCCCCTACT CACACCAGCC ACGCCTGGAG GCCTCCCACC TGCCGCTGCT
    GTGGCAGCTG CTGCAGCCAC TGCCAAGATC ACAGCTCAGG AAGCAGTGGC CGGAGCAGCG
    GTGCTGGGTA CCCTGGGCAC ACCTGGACTG GTGTCCCCAG CACTGACCCT GGCCCAGCCC
    CTGGGCACTT TGCCCCAGGC TGTCATGGCT GCCCAGGCAC CTGGAGTCAT CACAGGTGTG
    ACCCCAGCCC GTCCTCCTAT CCCGGTCACC ATCCCCTCGG TGGGAGTGGT GAACCCCATC
    CTGGCCAGCC CTCCAACGCT GGGTCTCCTG GAGCCCAAGA AGGAGAAGGA AGAAGAGGAG
    CTGTTTCCCG AGTCAGAGCG GCCAGAGATG CTGAGCGAGC AGGAGCACAT GAGCATCTCG
    GGCAGTAGCG CCCGACACAT GGTGATGCAG AAGCTGCTCC GCAAGCAGGA GTCTACAGTG
    ATGGTTCTGC GCAACATGGT GGACCCCAAG GACATCGATG ATGACCTGGA AGGGGAGGTG
    ACAGAGGAGT GTGGCAAGTT CGGGGCCGTG AACCGCGTCA TCATCTACCA AGAGAAACAA
    GGCGAGGAGG AGGATGCAGA AATCATTGTC AAGATCTTTG TGGAGTTTTC CATAGCCTCT
    GAGACTCATA AGGCCATCCA GGCCCTCAAT GGCCGCTGGT TTGCTGGCCG CAAGGTGGTG
    GCTGAAGTGT ACGACCAGGA GCGTTTTGAT AACAGTGACC TCTCTGCGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu64963
    Clone ID Related Accession (Same CDS sequence) XM_011516929.2 , NM_001362896.2 , NM_001362895.1 , NM_001362896.1 , XM_017013238.1 , XM_017013237.1 , XM_017013234.1 , NM_001362895.2
    Accession Version NM_001362895.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1791bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-10-21
    Organism Homo sapiens(human)
    Product poly(U)-binding-splicing factor PUF60 isoform i
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC234917.3, BG771821.1, BC011265.1, AC105219.6 and AW249619.1. On May 3, 2018 this sequence version replaced XM_017013238.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.57786.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGGAGCAGC TGAACTTGGC TGACAGTGGC CTGGTGGGGA AGGAAGCCAG AAGATGCAGA 
    AGGGCCTCCC CTCCCATGGG CATGCCTTCT GCCTGGTGGC TGCAGAATGA GGCCGGCTGC
    CTCTTTGCAG CAAGGCAGGT CAATGGCCAG CAAGGAGGGG GGTCCGAGCC GGCGGCGGCG
    GCGGCAGTGG TGGCAGCGGG AGACAAATGG AAACCTCCAC AGGGCACAGA CTCCATCAAG
    ATGGAGAACG GGCAGAGCAC AGCCGCCAAG CTGGGGCTGC CTCCCCTGAC GCCCGAGCAG
    CAGGAGGCCC TTCAGAAGGC CAAGAAGTAC GCCATGGAGC AGAGCATCAA GAGTGTGCTG
    GTGAAGCAGA CCATCGCGCA CCAGCAGCAG CAGCTCACCA ACCTGCAGAT GGCAGCAGTG
    ACAATGGGCT TTGGAGATCC TCTCTCACCT TTGCAATCGA TGGCGGCTCA GCGGCAGCGG
    GCGCTGGCCA TCATGTGCCG CGTCTACGTG GGCTCTATCT ACTATGAGCT GGGGGAGGAC
    ACCATCCGCC AGGCCTTTGC CCCCTTTGGC CCCATCAAGA GCATCGACAT GTCCTGGGAC
    TCCGTCACCA TGAAGCACAA GGGCTTTGCC TTCGTGGAGT ATGAGGTCCC CGAAGCTGCA
    CAGCTGGCCT TGGAGCAGAT GAACTCGGTG ATGCTGGGGG GCAGGAACAT CAAGGTGGGC
    AGACCCAGCA ACATAGGGCA GGCCCAGCCC ATCATAGACC AGTTGGCTGA GGAGGCACGG
    GCCTTCAACC GCATCTACGT GGCCTCTGTG CACCAGGACC TCTCAGACGA TGACATCAAG
    AGCGTGTTTG AGGCCTTTGG CAAGATCAAG TCCTGCACAC TGGCCCGGGA CCCCACAACT
    GGCAAGCACA AGGGCTACGG CTTCATTGAG TACGAGAAGG CCCAGTCGTC CCAAGATGCT
    GTGTCTTCCA TGAACCTCTT TGACCTGGGT GGCCAGTACT TGCGGGTGGG CAAGGCTGTC
    ACACCGCCCA TGCCCCTACT CACACCAGCC ACGCCTGGAG GCCTCCCACC TGCCGCTGCT
    GTGGCAGCTG CTGCAGCCAC TGCCAAGATC ACAGCTCAGG AAGCAGTGGC CGGAGCAGCG
    GTGCTGGGTA CCCTGGGCAC ACCTGGACTG GTGTCCCCAG CACTGACCCT GGCCCAGCCC
    CTGGGCACTT TGCCCCAGGC TGTCATGGCT GCCCAGGCAC CTGGAGTCAT CACAGGTGTG
    ACCCCAGCCC GTCCTCCTAT CCCGGTCACC ATCCCCTCGG TGGGAGTGGT GAACCCCATC
    CTGGCCAGCC CTCCAACGCT GGGTCTCCTG GAGCCCAAGA AGGAGAAGGA AGAAGAGGAG
    CTGTTTCCCG AGTCAGAGCG GCCAGAGATG CTGAGCGAGC AGGAGCACAT GAGCATCTCG
    GGCAGTAGCG CCCGACACAT GGTGATGCAG AAGCTGCTCC GCAAGCAGGA GTCTACAGTG
    ATGGTTCTGC GCAACATGGT GGACCCCAAG GACATCGATG ATGACCTGGA AGGGGAGGTG
    ACAGAGGAGT GTGGCAAGTT CGGGGCCGTG AACCGCGTCA TCATCTACCA AGAGAAACAA
    GGCGAGGAGG AGGATGCAGA AATCATTGTC AAGATCTTTG TGGAGTTTTC CATAGCCTCT
    GAGACTCATA AGGCCATCCA GGCCCTCAAT GGCCGCTGGT TTGCTGGCCG CAAGGTGGTG
    GCTGAAGTGT ACGACCAGGA GCGTTTTGAT AACAGTGACC TCTCTGCGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001349824.1
    CDS144..1934
    Translation

    Target ORF information:

    RefSeq Version NM_001362895.1
    Organism Homo sapiens(human)
    Definition Homo sapiens poly(U) binding splicing factor 60 (PUF60), transcript variant 9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001362895.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGGAGCAGC TGAACTTGGC TGACAGTGGC CTGGTGGGGA AGGAAGCCAG AAGATGCAGA 
    AGGGCCTCCC CTCCCATGGG CATGCCTTCT GCCTGGTGGC TGCAGAATGA GGCCGGCTGC
    CTCTTTGCAG CAAGGCAGGT CAATGGCCAG CAAGGAGGGG GGTCCGAGCC GGCGGCGGCG
    GCGGCAGTGG TGGCAGCGGG AGACAAATGG AAACCTCCAC AGGGCACAGA CTCCATCAAG
    ATGGAGAACG GGCAGAGCAC AGCCGCCAAG CTGGGGCTGC CTCCCCTGAC GCCCGAGCAG
    CAGGAGGCCC TTCAGAAGGC CAAGAAGTAC GCCATGGAGC AGAGCATCAA GAGTGTGCTG
    GTGAAGCAGA CCATCGCGCA CCAGCAGCAG CAGCTCACCA ACCTGCAGAT GGCAGCAGTG
    ACAATGGGCT TTGGAGATCC TCTCTCACCT TTGCAATCGA TGGCGGCTCA GCGGCAGCGG
    GCGCTGGCCA TCATGTGCCG CGTCTACGTG GGCTCTATCT ACTATGAGCT GGGGGAGGAC
    ACCATCCGCC AGGCCTTTGC CCCCTTTGGC CCCATCAAGA GCATCGACAT GTCCTGGGAC
    TCCGTCACCA TGAAGCACAA GGGCTTTGCC TTCGTGGAGT ATGAGGTCCC CGAAGCTGCA
    CAGCTGGCCT TGGAGCAGAT GAACTCGGTG ATGCTGGGGG GCAGGAACAT CAAGGTGGGC
    AGACCCAGCA ACATAGGGCA GGCCCAGCCC ATCATAGACC AGTTGGCTGA GGAGGCACGG
    GCCTTCAACC GCATCTACGT GGCCTCTGTG CACCAGGACC TCTCAGACGA TGACATCAAG
    AGCGTGTTTG AGGCCTTTGG CAAGATCAAG TCCTGCACAC TGGCCCGGGA CCCCACAACT
    GGCAAGCACA AGGGCTACGG CTTCATTGAG TACGAGAAGG CCCAGTCGTC CCAAGATGCT
    GTGTCTTCCA TGAACCTCTT TGACCTGGGT GGCCAGTACT TGCGGGTGGG CAAGGCTGTC
    ACACCGCCCA TGCCCCTACT CACACCAGCC ACGCCTGGAG GCCTCCCACC TGCCGCTGCT
    GTGGCAGCTG CTGCAGCCAC TGCCAAGATC ACAGCTCAGG AAGCAGTGGC CGGAGCAGCG
    GTGCTGGGTA CCCTGGGCAC ACCTGGACTG GTGTCCCCAG CACTGACCCT GGCCCAGCCC
    CTGGGCACTT TGCCCCAGGC TGTCATGGCT GCCCAGGCAC CTGGAGTCAT CACAGGTGTG
    ACCCCAGCCC GTCCTCCTAT CCCGGTCACC ATCCCCTCGG TGGGAGTGGT GAACCCCATC
    CTGGCCAGCC CTCCAACGCT GGGTCTCCTG GAGCCCAAGA AGGAGAAGGA AGAAGAGGAG
    CTGTTTCCCG AGTCAGAGCG GCCAGAGATG CTGAGCGAGC AGGAGCACAT GAGCATCTCG
    GGCAGTAGCG CCCGACACAT GGTGATGCAG AAGCTGCTCC GCAAGCAGGA GTCTACAGTG
    ATGGTTCTGC GCAACATGGT GGACCCCAAG GACATCGATG ATGACCTGGA AGGGGAGGTG
    ACAGAGGAGT GTGGCAAGTT CGGGGCCGTG AACCGCGTCA TCATCTACCA AGAGAAACAA
    GGCGAGGAGG AGGATGCAGA AATCATTGTC AAGATCTTTG TGGAGTTTTC CATAGCCTCT
    GAGACTCATA AGGCCATCCA GGCCCTCAAT GGCCGCTGGT TTGCTGGCCG CAAGGTGGTG
    GCTGAAGTGT ACGACCAGGA GCGTTTTGAT AACAGTGACC TCTCTGCGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu64963
    Clone ID Related Accession (Same CDS sequence) XM_011516929.2 , NM_001362896.2 , NM_001362895.1 , NM_001362896.1 , XM_017013238.1 , XM_017013237.1 , XM_017013234.1 , NM_001362895.2
    Accession Version NM_001362896.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1791bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-10-21
    Organism Homo sapiens(human)
    Product poly(U)-binding-splicing factor PUF60 isoform i
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC234917.3, BG771821.1, BC011265.1, AC105219.6 and AW249619.1. On May 3, 2018 this sequence version replaced XM_017013237.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.223171.1, SRR1660803.198393.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGGAGCAGC TGAACTTGGC TGACAGTGGC CTGGTGGGGA AGGAAGCCAG AAGATGCAGA 
    AGGGCCTCCC CTCCCATGGG CATGCCTTCT GCCTGGTGGC TGCAGAATGA GGCCGGCTGC
    CTCTTTGCAG CAAGGCAGGT CAATGGCCAG CAAGGAGGGG GGTCCGAGCC GGCGGCGGCG
    GCGGCAGTGG TGGCAGCGGG AGACAAATGG AAACCTCCAC AGGGCACAGA CTCCATCAAG
    ATGGAGAACG GGCAGAGCAC AGCCGCCAAG CTGGGGCTGC CTCCCCTGAC GCCCGAGCAG
    CAGGAGGCCC TTCAGAAGGC CAAGAAGTAC GCCATGGAGC AGAGCATCAA GAGTGTGCTG
    GTGAAGCAGA CCATCGCGCA CCAGCAGCAG CAGCTCACCA ACCTGCAGAT GGCAGCAGTG
    ACAATGGGCT TTGGAGATCC TCTCTCACCT TTGCAATCGA TGGCGGCTCA GCGGCAGCGG
    GCGCTGGCCA TCATGTGCCG CGTCTACGTG GGCTCTATCT ACTATGAGCT GGGGGAGGAC
    ACCATCCGCC AGGCCTTTGC CCCCTTTGGC CCCATCAAGA GCATCGACAT GTCCTGGGAC
    TCCGTCACCA TGAAGCACAA GGGCTTTGCC TTCGTGGAGT ATGAGGTCCC CGAAGCTGCA
    CAGCTGGCCT TGGAGCAGAT GAACTCGGTG ATGCTGGGGG GCAGGAACAT CAAGGTGGGC
    AGACCCAGCA ACATAGGGCA GGCCCAGCCC ATCATAGACC AGTTGGCTGA GGAGGCACGG
    GCCTTCAACC GCATCTACGT GGCCTCTGTG CACCAGGACC TCTCAGACGA TGACATCAAG
    AGCGTGTTTG AGGCCTTTGG CAAGATCAAG TCCTGCACAC TGGCCCGGGA CCCCACAACT
    GGCAAGCACA AGGGCTACGG CTTCATTGAG TACGAGAAGG CCCAGTCGTC CCAAGATGCT
    GTGTCTTCCA TGAACCTCTT TGACCTGGGT GGCCAGTACT TGCGGGTGGG CAAGGCTGTC
    ACACCGCCCA TGCCCCTACT CACACCAGCC ACGCCTGGAG GCCTCCCACC TGCCGCTGCT
    GTGGCAGCTG CTGCAGCCAC TGCCAAGATC ACAGCTCAGG AAGCAGTGGC CGGAGCAGCG
    GTGCTGGGTA CCCTGGGCAC ACCTGGACTG GTGTCCCCAG CACTGACCCT GGCCCAGCCC
    CTGGGCACTT TGCCCCAGGC TGTCATGGCT GCCCAGGCAC CTGGAGTCAT CACAGGTGTG
    ACCCCAGCCC GTCCTCCTAT CCCGGTCACC ATCCCCTCGG TGGGAGTGGT GAACCCCATC
    CTGGCCAGCC CTCCAACGCT GGGTCTCCTG GAGCCCAAGA AGGAGAAGGA AGAAGAGGAG
    CTGTTTCCCG AGTCAGAGCG GCCAGAGATG CTGAGCGAGC AGGAGCACAT GAGCATCTCG
    GGCAGTAGCG CCCGACACAT GGTGATGCAG AAGCTGCTCC GCAAGCAGGA GTCTACAGTG
    ATGGTTCTGC GCAACATGGT GGACCCCAAG GACATCGATG ATGACCTGGA AGGGGAGGTG
    ACAGAGGAGT GTGGCAAGTT CGGGGCCGTG AACCGCGTCA TCATCTACCA AGAGAAACAA
    GGCGAGGAGG AGGATGCAGA AATCATTGTC AAGATCTTTG TGGAGTTTTC CATAGCCTCT
    GAGACTCATA AGGCCATCCA GGCCCTCAAT GGCCGCTGGT TTGCTGGCCG CAAGGTGGTG
    GCTGAAGTGT ACGACCAGGA GCGTTTTGAT AACAGTGACC TCTCTGCGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001349825.1
    CDS307..2097
    Translation

    Target ORF information:

    RefSeq Version NM_001362896.1
    Organism Homo sapiens(human)
    Definition Homo sapiens poly(U) binding splicing factor 60 (PUF60), transcript variant 10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001362896.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGGAGCAGC TGAACTTGGC TGACAGTGGC CTGGTGGGGA AGGAAGCCAG AAGATGCAGA 
    AGGGCCTCCC CTCCCATGGG CATGCCTTCT GCCTGGTGGC TGCAGAATGA GGCCGGCTGC
    CTCTTTGCAG CAAGGCAGGT CAATGGCCAG CAAGGAGGGG GGTCCGAGCC GGCGGCGGCG
    GCGGCAGTGG TGGCAGCGGG AGACAAATGG AAACCTCCAC AGGGCACAGA CTCCATCAAG
    ATGGAGAACG GGCAGAGCAC AGCCGCCAAG CTGGGGCTGC CTCCCCTGAC GCCCGAGCAG
    CAGGAGGCCC TTCAGAAGGC CAAGAAGTAC GCCATGGAGC AGAGCATCAA GAGTGTGCTG
    GTGAAGCAGA CCATCGCGCA CCAGCAGCAG CAGCTCACCA ACCTGCAGAT GGCAGCAGTG
    ACAATGGGCT TTGGAGATCC TCTCTCACCT TTGCAATCGA TGGCGGCTCA GCGGCAGCGG
    GCGCTGGCCA TCATGTGCCG CGTCTACGTG GGCTCTATCT ACTATGAGCT GGGGGAGGAC
    ACCATCCGCC AGGCCTTTGC CCCCTTTGGC CCCATCAAGA GCATCGACAT GTCCTGGGAC
    TCCGTCACCA TGAAGCACAA GGGCTTTGCC TTCGTGGAGT ATGAGGTCCC CGAAGCTGCA
    CAGCTGGCCT TGGAGCAGAT GAACTCGGTG ATGCTGGGGG GCAGGAACAT CAAGGTGGGC
    AGACCCAGCA ACATAGGGCA GGCCCAGCCC ATCATAGACC AGTTGGCTGA GGAGGCACGG
    GCCTTCAACC GCATCTACGT GGCCTCTGTG CACCAGGACC TCTCAGACGA TGACATCAAG
    AGCGTGTTTG AGGCCTTTGG CAAGATCAAG TCCTGCACAC TGGCCCGGGA CCCCACAACT
    GGCAAGCACA AGGGCTACGG CTTCATTGAG TACGAGAAGG CCCAGTCGTC CCAAGATGCT
    GTGTCTTCCA TGAACCTCTT TGACCTGGGT GGCCAGTACT TGCGGGTGGG CAAGGCTGTC
    ACACCGCCCA TGCCCCTACT CACACCAGCC ACGCCTGGAG GCCTCCCACC TGCCGCTGCT
    GTGGCAGCTG CTGCAGCCAC TGCCAAGATC ACAGCTCAGG AAGCAGTGGC CGGAGCAGCG
    GTGCTGGGTA CCCTGGGCAC ACCTGGACTG GTGTCCCCAG CACTGACCCT GGCCCAGCCC
    CTGGGCACTT TGCCCCAGGC TGTCATGGCT GCCCAGGCAC CTGGAGTCAT CACAGGTGTG
    ACCCCAGCCC GTCCTCCTAT CCCGGTCACC ATCCCCTCGG TGGGAGTGGT GAACCCCATC
    CTGGCCAGCC CTCCAACGCT GGGTCTCCTG GAGCCCAAGA AGGAGAAGGA AGAAGAGGAG
    CTGTTTCCCG AGTCAGAGCG GCCAGAGATG CTGAGCGAGC AGGAGCACAT GAGCATCTCG
    GGCAGTAGCG CCCGACACAT GGTGATGCAG AAGCTGCTCC GCAAGCAGGA GTCTACAGTG
    ATGGTTCTGC GCAACATGGT GGACCCCAAG GACATCGATG ATGACCTGGA AGGGGAGGTG
    ACAGAGGAGT GTGGCAAGTT CGGGGCCGTG AACCGCGTCA TCATCTACCA AGAGAAACAA
    GGCGAGGAGG AGGATGCAGA AATCATTGTC AAGATCTTTG TGGAGTTTTC CATAGCCTCT
    GAGACTCATA AGGCCATCCA GGCCCTCAAT GGCCGCTGGT TTGCTGGCCG CAAGGTGGTG
    GCTGAAGTGT ACGACCAGGA GCGTTTTGAT AACAGTGACC TCTCTGCGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu64963
    Clone ID Related Accession (Same CDS sequence) XM_011516929.2 , NM_001362896.2 , NM_001362895.1 , NM_001362896.1 , XM_017013238.1 , XM_017013237.1 , XM_017013234.1 , NM_001362895.2
    Accession Version NM_001362895.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1791bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-27
    Organism Homo sapiens(human)
    Product poly(U)-binding-splicing factor PUF60 isoform i
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BG771821.1, BC011265.1, AC105219.6 and AW249619.1. On May 31, 2019 this sequence version replaced NM_001362895.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.57786.1, SRR7346977.97285.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGGAGCAGC TGAACTTGGC TGACAGTGGC CTGGTGGGGA AGGAAGCCAG AAGATGCAGA 
    AGGGCCTCCC CTCCCATGGG CATGCCTTCT GCCTGGTGGC TGCAGAATGA GGCCGGCTGC
    CTCTTTGCAG CAAGGCAGGT CAATGGCCAG CAAGGAGGGG GGTCCGAGCC GGCGGCGGCG
    GCGGCAGTGG TGGCAGCGGG AGACAAATGG AAACCTCCAC AGGGCACAGA CTCCATCAAG
    ATGGAGAACG GGCAGAGCAC AGCCGCCAAG CTGGGGCTGC CTCCCCTGAC GCCCGAGCAG
    CAGGAGGCCC TTCAGAAGGC CAAGAAGTAC GCCATGGAGC AGAGCATCAA GAGTGTGCTG
    GTGAAGCAGA CCATCGCGCA CCAGCAGCAG CAGCTCACCA ACCTGCAGAT GGCAGCAGTG
    ACAATGGGCT TTGGAGATCC TCTCTCACCT TTGCAATCGA TGGCGGCTCA GCGGCAGCGG
    GCGCTGGCCA TCATGTGCCG CGTCTACGTG GGCTCTATCT ACTATGAGCT GGGGGAGGAC
    ACCATCCGCC AGGCCTTTGC CCCCTTTGGC CCCATCAAGA GCATCGACAT GTCCTGGGAC
    TCCGTCACCA TGAAGCACAA GGGCTTTGCC TTCGTGGAGT ATGAGGTCCC CGAAGCTGCA
    CAGCTGGCCT TGGAGCAGAT GAACTCGGTG ATGCTGGGGG GCAGGAACAT CAAGGTGGGC
    AGACCCAGCA ACATAGGGCA GGCCCAGCCC ATCATAGACC AGTTGGCTGA GGAGGCACGG
    GCCTTCAACC GCATCTACGT GGCCTCTGTG CACCAGGACC TCTCAGACGA TGACATCAAG
    AGCGTGTTTG AGGCCTTTGG CAAGATCAAG TCCTGCACAC TGGCCCGGGA CCCCACAACT
    GGCAAGCACA AGGGCTACGG CTTCATTGAG TACGAGAAGG CCCAGTCGTC CCAAGATGCT
    GTGTCTTCCA TGAACCTCTT TGACCTGGGT GGCCAGTACT TGCGGGTGGG CAAGGCTGTC
    ACACCGCCCA TGCCCCTACT CACACCAGCC ACGCCTGGAG GCCTCCCACC TGCCGCTGCT
    GTGGCAGCTG CTGCAGCCAC TGCCAAGATC ACAGCTCAGG AAGCAGTGGC CGGAGCAGCG
    GTGCTGGGTA CCCTGGGCAC ACCTGGACTG GTGTCCCCAG CACTGACCCT GGCCCAGCCC
    CTGGGCACTT TGCCCCAGGC TGTCATGGCT GCCCAGGCAC CTGGAGTCAT CACAGGTGTG
    ACCCCAGCCC GTCCTCCTAT CCCGGTCACC ATCCCCTCGG TGGGAGTGGT GAACCCCATC
    CTGGCCAGCC CTCCAACGCT GGGTCTCCTG GAGCCCAAGA AGGAGAAGGA AGAAGAGGAG
    CTGTTTCCCG AGTCAGAGCG GCCAGAGATG CTGAGCGAGC AGGAGCACAT GAGCATCTCG
    GGCAGTAGCG CCCGACACAT GGTGATGCAG AAGCTGCTCC GCAAGCAGGA GTCTACAGTG
    ATGGTTCTGC GCAACATGGT GGACCCCAAG GACATCGATG ATGACCTGGA AGGGGAGGTG
    ACAGAGGAGT GTGGCAAGTT CGGGGCCGTG AACCGCGTCA TCATCTACCA AGAGAAACAA
    GGCGAGGAGG AGGATGCAGA AATCATTGTC AAGATCTTTG TGGAGTTTTC CATAGCCTCT
    GAGACTCATA AGGCCATCCA GGCCCTCAAT GGCCGCTGGT TTGCTGGCCG CAAGGTGGTG
    GCTGAAGTGT ACGACCAGGA GCGTTTTGAT AACAGTGACC TCTCTGCGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001349824.1
    CDS76..1866
    Translation

    Target ORF information:

    RefSeq Version NM_001362895.2
    Organism Homo sapiens(human)
    Definition Homo sapiens poly(U) binding splicing factor 60 (PUF60), transcript variant 9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001362895.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGGAGCAGC TGAACTTGGC TGACAGTGGC CTGGTGGGGA AGGAAGCCAG AAGATGCAGA 
    AGGGCCTCCC CTCCCATGGG CATGCCTTCT GCCTGGTGGC TGCAGAATGA GGCCGGCTGC
    CTCTTTGCAG CAAGGCAGGT CAATGGCCAG CAAGGAGGGG GGTCCGAGCC GGCGGCGGCG
    GCGGCAGTGG TGGCAGCGGG AGACAAATGG AAACCTCCAC AGGGCACAGA CTCCATCAAG
    ATGGAGAACG GGCAGAGCAC AGCCGCCAAG CTGGGGCTGC CTCCCCTGAC GCCCGAGCAG
    CAGGAGGCCC TTCAGAAGGC CAAGAAGTAC GCCATGGAGC AGAGCATCAA GAGTGTGCTG
    GTGAAGCAGA CCATCGCGCA CCAGCAGCAG CAGCTCACCA ACCTGCAGAT GGCAGCAGTG
    ACAATGGGCT TTGGAGATCC TCTCTCACCT TTGCAATCGA TGGCGGCTCA GCGGCAGCGG
    GCGCTGGCCA TCATGTGCCG CGTCTACGTG GGCTCTATCT ACTATGAGCT GGGGGAGGAC
    ACCATCCGCC AGGCCTTTGC CCCCTTTGGC CCCATCAAGA GCATCGACAT GTCCTGGGAC
    TCCGTCACCA TGAAGCACAA GGGCTTTGCC TTCGTGGAGT ATGAGGTCCC CGAAGCTGCA
    CAGCTGGCCT TGGAGCAGAT GAACTCGGTG ATGCTGGGGG GCAGGAACAT CAAGGTGGGC
    AGACCCAGCA ACATAGGGCA GGCCCAGCCC ATCATAGACC AGTTGGCTGA GGAGGCACGG
    GCCTTCAACC GCATCTACGT GGCCTCTGTG CACCAGGACC TCTCAGACGA TGACATCAAG
    AGCGTGTTTG AGGCCTTTGG CAAGATCAAG TCCTGCACAC TGGCCCGGGA CCCCACAACT
    GGCAAGCACA AGGGCTACGG CTTCATTGAG TACGAGAAGG CCCAGTCGTC CCAAGATGCT
    GTGTCTTCCA TGAACCTCTT TGACCTGGGT GGCCAGTACT TGCGGGTGGG CAAGGCTGTC
    ACACCGCCCA TGCCCCTACT CACACCAGCC ACGCCTGGAG GCCTCCCACC TGCCGCTGCT
    GTGGCAGCTG CTGCAGCCAC TGCCAAGATC ACAGCTCAGG AAGCAGTGGC CGGAGCAGCG
    GTGCTGGGTA CCCTGGGCAC ACCTGGACTG GTGTCCCCAG CACTGACCCT GGCCCAGCCC
    CTGGGCACTT TGCCCCAGGC TGTCATGGCT GCCCAGGCAC CTGGAGTCAT CACAGGTGTG
    ACCCCAGCCC GTCCTCCTAT CCCGGTCACC ATCCCCTCGG TGGGAGTGGT GAACCCCATC
    CTGGCCAGCC CTCCAACGCT GGGTCTCCTG GAGCCCAAGA AGGAGAAGGA AGAAGAGGAG
    CTGTTTCCCG AGTCAGAGCG GCCAGAGATG CTGAGCGAGC AGGAGCACAT GAGCATCTCG
    GGCAGTAGCG CCCGACACAT GGTGATGCAG AAGCTGCTCC GCAAGCAGGA GTCTACAGTG
    ATGGTTCTGC GCAACATGGT GGACCCCAAG GACATCGATG ATGACCTGGA AGGGGAGGTG
    ACAGAGGAGT GTGGCAAGTT CGGGGCCGTG AACCGCGTCA TCATCTACCA AGAGAAACAA
    GGCGAGGAGG AGGATGCAGA AATCATTGTC AAGATCTTTG TGGAGTTTTC CATAGCCTCT
    GAGACTCATA AGGCCATCCA GGCCCTCAAT GGCCGCTGGT TTGCTGGCCG CAAGGTGGTG
    GCTGAAGTGT ACGACCAGGA GCGTTTTGAT AACAGTGACC TCTCTGCGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu64964
    Clone ID Related Accession (Same CDS sequence) NM_001362897.1 , XM_017013236.1 , NM_001362897.2 , XM_011516930.2
    Accession Version NM_001362897.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1740bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-10-21
    Organism Homo sapiens(human)
    Product poly(U)-binding-splicing factor PUF60 isoform j
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC234917.3, BG771821.1, BQ421738.1, BC011265.1 and AW249619.1. On May 3, 2018 this sequence version replaced XM_011516930.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.156133.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGGAGCAGC TGAACTTGGC TGACAGTGGC CTGGTGGGGA AGGAAGCCAG AAGATGCAGA 
    AGGGCCTCCC CTCCCATGGG CATGCCTTCT GCCTGGTGGC TGCAGAATGA GGCCGGCTGC
    CTCTTTGCAG CAAGGCAGGT CAATGGCCAG CAAGGAGGGG GGTCCGAGCC GGCGGCGGCG
    GCGGCAGTGG TGGCAGCGGG AGACAAATGG AAACCTCCAC AGGGCACAGA CTCCATCAAG
    ATGGAGAACG GGCAGAGCAC AGCCGCCAAG CTGGGGCTGC CTCCCCTGAC GCCCGAGCAG
    CAGGAGGCCC TTCAGAAGGC CAAGAAGTAC GCCATGGAGC AGAGCATCAA GAGTGTGCTG
    GTGAAGCAGA CCATCGCGCA CCAGCAGCAG CAGCTCACCA ACCTGCAGAT GGCGGCTCAG
    CGGCAGCGGG CGCTGGCCAT CATGTGCCGC GTCTACGTGG GCTCTATCTA CTATGAGCTG
    GGGGAGGACA CCATCCGCCA GGCCTTTGCC CCCTTTGGCC CCATCAAGAG CATCGACATG
    TCCTGGGACT CCGTCACCAT GAAGCACAAG GGCTTTGCCT TCGTGGAGTA TGAGGTCCCC
    GAAGCTGCAC AGCTGGCCTT GGAGCAGATG AACTCGGTGA TGCTGGGGGG CAGGAACATC
    AAGGTGGGCA GACCCAGCAA CATAGGGCAG GCCCAGCCCA TCATAGACCA GTTGGCTGAG
    GAGGCACGGG CCTTCAACCG CATCTACGTG GCCTCTGTGC ACCAGGACCT CTCAGACGAT
    GACATCAAGA GCGTGTTTGA GGCCTTTGGC AAGATCAAGT CCTGCACACT GGCCCGGGAC
    CCCACAACTG GCAAGCACAA GGGCTACGGC TTCATTGAGT ACGAGAAGGC CCAGTCGTCC
    CAAGATGCTG TGTCTTCCAT GAACCTCTTT GACCTGGGTG GCCAGTACTT GCGGGTGGGC
    AAGGCTGTCA CACCGCCCAT GCCCCTACTC ACACCAGCCA CGCCTGGAGG CCTCCCACCT
    GCCGCTGCTG TGGCAGCTGC TGCAGCCACT GCCAAGATCA CAGCTCAGGA AGCAGTGGCC
    GGAGCAGCGG TGCTGGGTAC CCTGGGCACA CCTGGACTGG TGTCCCCAGC ACTGACCCTG
    GCCCAGCCCC TGGGCACTTT GCCCCAGGCT GTCATGGCTG CCCAGGCACC TGGAGTCATC
    ACAGGTGTGA CCCCAGCCCG TCCTCCTATC CCGGTCACCA TCCCCTCGGT GGGAGTGGTG
    AACCCCATCC TGGCCAGCCC TCCAACGCTG GGTCTCCTGG AGCCCAAGAA GGAGAAGGAA
    GAAGAGGAGC TGTTTCCCGA GTCAGAGCGG CCAGAGATGC TGAGCGAGCA GGAGCACATG
    AGCATCTCGG GCAGTAGCGC CCGACACATG GTGATGCAGA AGCTGCTCCG CAAGCAGGAG
    TCTACAGTGA TGGTTCTGCG CAACATGGTG GACCCCAAGG ACATCGATGA TGACCTGGAA
    GGGGAGGTGA CAGAGGAGTG TGGCAAGTTC GGGGCCGTGA ACCGCGTCAT CATCTACCAA
    GAGAAACAAG GCGAGGAGGA GGATGCAGAA ATCATTGTCA AGATCTTTGT GGAGTTTTCC
    ATAGCCTCTG AGACTCATAA GGCCATCCAG GCCCTCAATG GCCGCTGGTT TGCTGGCCGC
    AAGGTGGTGG CTGAAGTGTA CGACCAGGAG CGTTTTGATA ACAGTGACCT CTCTGCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001349826.1
    CDS144..1883
    Translation

    Target ORF information:

    RefSeq Version NM_001362897.1
    Organism Homo sapiens(human)
    Definition Homo sapiens poly(U) binding splicing factor 60 (PUF60), transcript variant 11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001362897.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGGAGCAGC TGAACTTGGC TGACAGTGGC CTGGTGGGGA AGGAAGCCAG AAGATGCAGA 
    AGGGCCTCCC CTCCCATGGG CATGCCTTCT GCCTGGTGGC TGCAGAATGA GGCCGGCTGC
    CTCTTTGCAG CAAGGCAGGT CAATGGCCAG CAAGGAGGGG GGTCCGAGCC GGCGGCGGCG
    GCGGCAGTGG TGGCAGCGGG AGACAAATGG AAACCTCCAC AGGGCACAGA CTCCATCAAG
    ATGGAGAACG GGCAGAGCAC AGCCGCCAAG CTGGGGCTGC CTCCCCTGAC GCCCGAGCAG
    CAGGAGGCCC TTCAGAAGGC CAAGAAGTAC GCCATGGAGC AGAGCATCAA GAGTGTGCTG
    GTGAAGCAGA CCATCGCGCA CCAGCAGCAG CAGCTCACCA ACCTGCAGAT GGCGGCTCAG
    CGGCAGCGGG CGCTGGCCAT CATGTGCCGC GTCTACGTGG GCTCTATCTA CTATGAGCTG
    GGGGAGGACA CCATCCGCCA GGCCTTTGCC CCCTTTGGCC CCATCAAGAG CATCGACATG
    TCCTGGGACT CCGTCACCAT GAAGCACAAG GGCTTTGCCT TCGTGGAGTA TGAGGTCCCC
    GAAGCTGCAC AGCTGGCCTT GGAGCAGATG AACTCGGTGA TGCTGGGGGG CAGGAACATC
    AAGGTGGGCA GACCCAGCAA CATAGGGCAG GCCCAGCCCA TCATAGACCA GTTGGCTGAG
    GAGGCACGGG CCTTCAACCG CATCTACGTG GCCTCTGTGC ACCAGGACCT CTCAGACGAT
    GACATCAAGA GCGTGTTTGA GGCCTTTGGC AAGATCAAGT CCTGCACACT GGCCCGGGAC
    CCCACAACTG GCAAGCACAA GGGCTACGGC TTCATTGAGT ACGAGAAGGC CCAGTCGTCC
    CAAGATGCTG TGTCTTCCAT GAACCTCTTT GACCTGGGTG GCCAGTACTT GCGGGTGGGC
    AAGGCTGTCA CACCGCCCAT GCCCCTACTC ACACCAGCCA CGCCTGGAGG CCTCCCACCT
    GCCGCTGCTG TGGCAGCTGC TGCAGCCACT GCCAAGATCA CAGCTCAGGA AGCAGTGGCC
    GGAGCAGCGG TGCTGGGTAC CCTGGGCACA CCTGGACTGG TGTCCCCAGC ACTGACCCTG
    GCCCAGCCCC TGGGCACTTT GCCCCAGGCT GTCATGGCTG CCCAGGCACC TGGAGTCATC
    ACAGGTGTGA CCCCAGCCCG TCCTCCTATC CCGGTCACCA TCCCCTCGGT GGGAGTGGTG
    AACCCCATCC TGGCCAGCCC TCCAACGCTG GGTCTCCTGG AGCCCAAGAA GGAGAAGGAA
    GAAGAGGAGC TGTTTCCCGA GTCAGAGCGG CCAGAGATGC TGAGCGAGCA GGAGCACATG
    AGCATCTCGG GCAGTAGCGC CCGACACATG GTGATGCAGA AGCTGCTCCG CAAGCAGGAG
    TCTACAGTGA TGGTTCTGCG CAACATGGTG GACCCCAAGG ACATCGATGA TGACCTGGAA
    GGGGAGGTGA CAGAGGAGTG TGGCAAGTTC GGGGCCGTGA ACCGCGTCAT CATCTACCAA
    GAGAAACAAG GCGAGGAGGA GGATGCAGAA ATCATTGTCA AGATCTTTGT GGAGTTTTCC
    ATAGCCTCTG AGACTCATAA GGCCATCCAG GCCCTCAATG GCCGCTGGTT TGCTGGCCGC
    AAGGTGGTGG CTGAAGTGTA CGACCAGGAG CGTTTTGATA ACAGTGACCT CTCTGCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu64964
    Clone ID Related Accession (Same CDS sequence) NM_001362897.1 , XM_017013236.1 , NM_001362897.2 , XM_011516930.2
    Accession Version NM_001362897.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1740bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-27
    Organism Homo sapiens(human)
    Product poly(U)-binding-splicing factor PUF60 isoform j
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BQ421738.1, BC011265.1 and AW249619.1. On May 31, 2019 this sequence version replaced NM_001362897.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.156133.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGGAGCAGC TGAACTTGGC TGACAGTGGC CTGGTGGGGA AGGAAGCCAG AAGATGCAGA 
    AGGGCCTCCC CTCCCATGGG CATGCCTTCT GCCTGGTGGC TGCAGAATGA GGCCGGCTGC
    CTCTTTGCAG CAAGGCAGGT CAATGGCCAG CAAGGAGGGG GGTCCGAGCC GGCGGCGGCG
    GCGGCAGTGG TGGCAGCGGG AGACAAATGG AAACCTCCAC AGGGCACAGA CTCCATCAAG
    ATGGAGAACG GGCAGAGCAC AGCCGCCAAG CTGGGGCTGC CTCCCCTGAC GCCCGAGCAG
    CAGGAGGCCC TTCAGAAGGC CAAGAAGTAC GCCATGGAGC AGAGCATCAA GAGTGTGCTG
    GTGAAGCAGA CCATCGCGCA CCAGCAGCAG CAGCTCACCA ACCTGCAGAT GGCGGCTCAG
    CGGCAGCGGG CGCTGGCCAT CATGTGCCGC GTCTACGTGG GCTCTATCTA CTATGAGCTG
    GGGGAGGACA CCATCCGCCA GGCCTTTGCC CCCTTTGGCC CCATCAAGAG CATCGACATG
    TCCTGGGACT CCGTCACCAT GAAGCACAAG GGCTTTGCCT TCGTGGAGTA TGAGGTCCCC
    GAAGCTGCAC AGCTGGCCTT GGAGCAGATG AACTCGGTGA TGCTGGGGGG CAGGAACATC
    AAGGTGGGCA GACCCAGCAA CATAGGGCAG GCCCAGCCCA TCATAGACCA GTTGGCTGAG
    GAGGCACGGG CCTTCAACCG CATCTACGTG GCCTCTGTGC ACCAGGACCT CTCAGACGAT
    GACATCAAGA GCGTGTTTGA GGCCTTTGGC AAGATCAAGT CCTGCACACT GGCCCGGGAC
    CCCACAACTG GCAAGCACAA GGGCTACGGC TTCATTGAGT ACGAGAAGGC CCAGTCGTCC
    CAAGATGCTG TGTCTTCCAT GAACCTCTTT GACCTGGGTG GCCAGTACTT GCGGGTGGGC
    AAGGCTGTCA CACCGCCCAT GCCCCTACTC ACACCAGCCA CGCCTGGAGG CCTCCCACCT
    GCCGCTGCTG TGGCAGCTGC TGCAGCCACT GCCAAGATCA CAGCTCAGGA AGCAGTGGCC
    GGAGCAGCGG TGCTGGGTAC CCTGGGCACA CCTGGACTGG TGTCCCCAGC ACTGACCCTG
    GCCCAGCCCC TGGGCACTTT GCCCCAGGCT GTCATGGCTG CCCAGGCACC TGGAGTCATC
    ACAGGTGTGA CCCCAGCCCG TCCTCCTATC CCGGTCACCA TCCCCTCGGT GGGAGTGGTG
    AACCCCATCC TGGCCAGCCC TCCAACGCTG GGTCTCCTGG AGCCCAAGAA GGAGAAGGAA
    GAAGAGGAGC TGTTTCCCGA GTCAGAGCGG CCAGAGATGC TGAGCGAGCA GGAGCACATG
    AGCATCTCGG GCAGTAGCGC CCGACACATG GTGATGCAGA AGCTGCTCCG CAAGCAGGAG
    TCTACAGTGA TGGTTCTGCG CAACATGGTG GACCCCAAGG ACATCGATGA TGACCTGGAA
    GGGGAGGTGA CAGAGGAGTG TGGCAAGTTC GGGGCCGTGA ACCGCGTCAT CATCTACCAA
    GAGAAACAAG GCGAGGAGGA GGATGCAGAA ATCATTGTCA AGATCTTTGT GGAGTTTTCC
    ATAGCCTCTG AGACTCATAA GGCCATCCAG GCCCTCAATG GCCGCTGGTT TGCTGGCCGC
    AAGGTGGTGG CTGAAGTGTA CGACCAGGAG CGTTTTGATA ACAGTGACCT CTCTGCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001349826.1
    CDS76..1815
    Translation

    Target ORF information:

    RefSeq Version NM_001362897.2
    Organism Homo sapiens(human)
    Definition Homo sapiens poly(U) binding splicing factor 60 (PUF60), transcript variant 11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001362897.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGGAGCAGC TGAACTTGGC TGACAGTGGC CTGGTGGGGA AGGAAGCCAG AAGATGCAGA 
    AGGGCCTCCC CTCCCATGGG CATGCCTTCT GCCTGGTGGC TGCAGAATGA GGCCGGCTGC
    CTCTTTGCAG CAAGGCAGGT CAATGGCCAG CAAGGAGGGG GGTCCGAGCC GGCGGCGGCG
    GCGGCAGTGG TGGCAGCGGG AGACAAATGG AAACCTCCAC AGGGCACAGA CTCCATCAAG
    ATGGAGAACG GGCAGAGCAC AGCCGCCAAG CTGGGGCTGC CTCCCCTGAC GCCCGAGCAG
    CAGGAGGCCC TTCAGAAGGC CAAGAAGTAC GCCATGGAGC AGAGCATCAA GAGTGTGCTG
    GTGAAGCAGA CCATCGCGCA CCAGCAGCAG CAGCTCACCA ACCTGCAGAT GGCGGCTCAG
    CGGCAGCGGG CGCTGGCCAT CATGTGCCGC GTCTACGTGG GCTCTATCTA CTATGAGCTG
    GGGGAGGACA CCATCCGCCA GGCCTTTGCC CCCTTTGGCC CCATCAAGAG CATCGACATG
    TCCTGGGACT CCGTCACCAT GAAGCACAAG GGCTTTGCCT TCGTGGAGTA TGAGGTCCCC
    GAAGCTGCAC AGCTGGCCTT GGAGCAGATG AACTCGGTGA TGCTGGGGGG CAGGAACATC
    AAGGTGGGCA GACCCAGCAA CATAGGGCAG GCCCAGCCCA TCATAGACCA GTTGGCTGAG
    GAGGCACGGG CCTTCAACCG CATCTACGTG GCCTCTGTGC ACCAGGACCT CTCAGACGAT
    GACATCAAGA GCGTGTTTGA GGCCTTTGGC AAGATCAAGT CCTGCACACT GGCCCGGGAC
    CCCACAACTG GCAAGCACAA GGGCTACGGC TTCATTGAGT ACGAGAAGGC CCAGTCGTCC
    CAAGATGCTG TGTCTTCCAT GAACCTCTTT GACCTGGGTG GCCAGTACTT GCGGGTGGGC
    AAGGCTGTCA CACCGCCCAT GCCCCTACTC ACACCAGCCA CGCCTGGAGG CCTCCCACCT
    GCCGCTGCTG TGGCAGCTGC TGCAGCCACT GCCAAGATCA CAGCTCAGGA AGCAGTGGCC
    GGAGCAGCGG TGCTGGGTAC CCTGGGCACA CCTGGACTGG TGTCCCCAGC ACTGACCCTG
    GCCCAGCCCC TGGGCACTTT GCCCCAGGCT GTCATGGCTG CCCAGGCACC TGGAGTCATC
    ACAGGTGTGA CCCCAGCCCG TCCTCCTATC CCGGTCACCA TCCCCTCGGT GGGAGTGGTG
    AACCCCATCC TGGCCAGCCC TCCAACGCTG GGTCTCCTGG AGCCCAAGAA GGAGAAGGAA
    GAAGAGGAGC TGTTTCCCGA GTCAGAGCGG CCAGAGATGC TGAGCGAGCA GGAGCACATG
    AGCATCTCGG GCAGTAGCGC CCGACACATG GTGATGCAGA AGCTGCTCCG CAAGCAGGAG
    TCTACAGTGA TGGTTCTGCG CAACATGGTG GACCCCAAGG ACATCGATGA TGACCTGGAA
    GGGGAGGTGA CAGAGGAGTG TGGCAAGTTC GGGGCCGTGA ACCGCGTCAT CATCTACCAA
    GAGAAACAAG GCGAGGAGGA GGATGCAGAA ATCATTGTCA AGATCTTTGT GGAGTTTTCC
    ATAGCCTCTG AGACTCATAA GGCCATCCAG GCCCTCAATG GCCGCTGGTT TGCTGGCCGC
    AAGGTGGTGG CTGAAGTGTA CGACCAGGAG CGTTTTGATA ACAGTGACCT CTCTGCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu64963
    Clone ID Related Accession (Same CDS sequence) XM_011516929.2 , NM_001362896.2 , NM_001362895.1 , NM_001362896.1 , XM_017013238.1 , XM_017013237.1 , XM_017013234.1 , NM_001362895.2
    Accession Version XM_011516929.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1791bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product poly(U)-binding-splicing factor PUF60 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000008.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011516929.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGGAGCAGC TGAACTTGGC TGACAGTGGC CTGGTGGGGA AGGAAGCCAG AAGATGCAGA 
    AGGGCCTCCC CTCCCATGGG CATGCCTTCT GCCTGGTGGC TGCAGAATGA GGCCGGCTGC
    CTCTTTGCAG CAAGGCAGGT CAATGGCCAG CAAGGAGGGG GGTCCGAGCC GGCGGCGGCG
    GCGGCAGTGG TGGCAGCGGG AGACAAATGG AAACCTCCAC AGGGCACAGA CTCCATCAAG
    ATGGAGAACG GGCAGAGCAC AGCCGCCAAG CTGGGGCTGC CTCCCCTGAC GCCCGAGCAG
    CAGGAGGCCC TTCAGAAGGC CAAGAAGTAC GCCATGGAGC AGAGCATCAA GAGTGTGCTG
    GTGAAGCAGA CCATCGCGCA CCAGCAGCAG CAGCTCACCA ACCTGCAGAT GGCAGCAGTG
    ACAATGGGCT TTGGAGATCC TCTCTCACCT TTGCAATCGA TGGCGGCTCA GCGGCAGCGG
    GCGCTGGCCA TCATGTGCCG CGTCTACGTG GGCTCTATCT ACTATGAGCT GGGGGAGGAC
    ACCATCCGCC AGGCCTTTGC CCCCTTTGGC CCCATCAAGA GCATCGACAT GTCCTGGGAC
    TCCGTCACCA TGAAGCACAA GGGCTTTGCC TTCGTGGAGT ATGAGGTCCC CGAAGCTGCA
    CAGCTGGCCT TGGAGCAGAT GAACTCGGTG ATGCTGGGGG GCAGGAACAT CAAGGTGGGC
    AGACCCAGCA ACATAGGGCA GGCCCAGCCC ATCATAGACC AGTTGGCTGA GGAGGCACGG
    GCCTTCAACC GCATCTACGT GGCCTCTGTG CACCAGGACC TCTCAGACGA TGACATCAAG
    AGCGTGTTTG AGGCCTTTGG CAAGATCAAG TCCTGCACAC TGGCCCGGGA CCCCACAACT
    GGCAAGCACA AGGGCTACGG CTTCATTGAG TACGAGAAGG CCCAGTCGTC CCAAGATGCT
    GTGTCTTCCA TGAACCTCTT TGACCTGGGT GGCCAGTACT TGCGGGTGGG CAAGGCTGTC
    ACACCGCCCA TGCCCCTACT CACACCAGCC ACGCCTGGAG GCCTCCCACC TGCCGCTGCT
    GTGGCAGCTG CTGCAGCCAC TGCCAAGATC ACAGCTCAGG AAGCAGTGGC CGGAGCAGCG
    GTGCTGGGTA CCCTGGGCAC ACCTGGACTG GTGTCCCCAG CACTGACCCT GGCCCAGCCC
    CTGGGCACTT TGCCCCAGGC TGTCATGGCT GCCCAGGCAC CTGGAGTCAT CACAGGTGTG
    ACCCCAGCCC GTCCTCCTAT CCCGGTCACC ATCCCCTCGG TGGGAGTGGT GAACCCCATC
    CTGGCCAGCC CTCCAACGCT GGGTCTCCTG GAGCCCAAGA AGGAGAAGGA AGAAGAGGAG
    CTGTTTCCCG AGTCAGAGCG GCCAGAGATG CTGAGCGAGC AGGAGCACAT GAGCATCTCG
    GGCAGTAGCG CCCGACACAT GGTGATGCAG AAGCTGCTCC GCAAGCAGGA GTCTACAGTG
    ATGGTTCTGC GCAACATGGT GGACCCCAAG GACATCGATG ATGACCTGGA AGGGGAGGTG
    ACAGAGGAGT GTGGCAAGTT CGGGGCCGTG AACCGCGTCA TCATCTACCA AGAGAAACAA
    GGCGAGGAGG AGGATGCAGA AATCATTGTC AAGATCTTTG TGGAGTTTTC CATAGCCTCT
    GAGACTCATA AGGCCATCCA GGCCCTCAAT GGCCGCTGGT TTGCTGGCCG CAAGGTGGTG
    GCTGAAGTGT ACGACCAGGA GCGTTTTGAT AACAGTGACC TCTCTGCGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011515231.1
    CDS172..1962
    Translation

    Target ORF information:

    RefSeq Version XM_011516929.2
    Organism Homo sapiens(human)
    Definition Homo sapiens poly(U) binding splicing factor 60KDa (PUF60), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011516929.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGGAGCAGC TGAACTTGGC TGACAGTGGC CTGGTGGGGA AGGAAGCCAG AAGATGCAGA 
    AGGGCCTCCC CTCCCATGGG CATGCCTTCT GCCTGGTGGC TGCAGAATGA GGCCGGCTGC
    CTCTTTGCAG CAAGGCAGGT CAATGGCCAG CAAGGAGGGG GGTCCGAGCC GGCGGCGGCG
    GCGGCAGTGG TGGCAGCGGG AGACAAATGG AAACCTCCAC AGGGCACAGA CTCCATCAAG
    ATGGAGAACG GGCAGAGCAC AGCCGCCAAG CTGGGGCTGC CTCCCCTGAC GCCCGAGCAG
    CAGGAGGCCC TTCAGAAGGC CAAGAAGTAC GCCATGGAGC AGAGCATCAA GAGTGTGCTG
    GTGAAGCAGA CCATCGCGCA CCAGCAGCAG CAGCTCACCA ACCTGCAGAT GGCAGCAGTG
    ACAATGGGCT TTGGAGATCC TCTCTCACCT TTGCAATCGA TGGCGGCTCA GCGGCAGCGG
    GCGCTGGCCA TCATGTGCCG CGTCTACGTG GGCTCTATCT ACTATGAGCT GGGGGAGGAC
    ACCATCCGCC AGGCCTTTGC CCCCTTTGGC CCCATCAAGA GCATCGACAT GTCCTGGGAC
    TCCGTCACCA TGAAGCACAA GGGCTTTGCC TTCGTGGAGT ATGAGGTCCC CGAAGCTGCA
    CAGCTGGCCT TGGAGCAGAT GAACTCGGTG ATGCTGGGGG GCAGGAACAT CAAGGTGGGC
    AGACCCAGCA ACATAGGGCA GGCCCAGCCC ATCATAGACC AGTTGGCTGA GGAGGCACGG
    GCCTTCAACC GCATCTACGT GGCCTCTGTG CACCAGGACC TCTCAGACGA TGACATCAAG
    AGCGTGTTTG AGGCCTTTGG CAAGATCAAG TCCTGCACAC TGGCCCGGGA CCCCACAACT
    GGCAAGCACA AGGGCTACGG CTTCATTGAG TACGAGAAGG CCCAGTCGTC CCAAGATGCT
    GTGTCTTCCA TGAACCTCTT TGACCTGGGT GGCCAGTACT TGCGGGTGGG CAAGGCTGTC
    ACACCGCCCA TGCCCCTACT CACACCAGCC ACGCCTGGAG GCCTCCCACC TGCCGCTGCT
    GTGGCAGCTG CTGCAGCCAC TGCCAAGATC ACAGCTCAGG AAGCAGTGGC CGGAGCAGCG
    GTGCTGGGTA CCCTGGGCAC ACCTGGACTG GTGTCCCCAG CACTGACCCT GGCCCAGCCC
    CTGGGCACTT TGCCCCAGGC TGTCATGGCT GCCCAGGCAC CTGGAGTCAT CACAGGTGTG
    ACCCCAGCCC GTCCTCCTAT CCCGGTCACC ATCCCCTCGG TGGGAGTGGT GAACCCCATC
    CTGGCCAGCC CTCCAACGCT GGGTCTCCTG GAGCCCAAGA AGGAGAAGGA AGAAGAGGAG
    CTGTTTCCCG AGTCAGAGCG GCCAGAGATG CTGAGCGAGC AGGAGCACAT GAGCATCTCG
    GGCAGTAGCG CCCGACACAT GGTGATGCAG AAGCTGCTCC GCAAGCAGGA GTCTACAGTG
    ATGGTTCTGC GCAACATGGT GGACCCCAAG GACATCGATG ATGACCTGGA AGGGGAGGTG
    ACAGAGGAGT GTGGCAAGTT CGGGGCCGTG AACCGCGTCA TCATCTACCA AGAGAAACAA
    GGCGAGGAGG AGGATGCAGA AATCATTGTC AAGATCTTTG TGGAGTTTTC CATAGCCTCT
    GAGACTCATA AGGCCATCCA GGCCCTCAAT GGCCGCTGGT TTGCTGGCCG CAAGGTGGTG
    GCTGAAGTGT ACGACCAGGA GCGTTTTGAT AACAGTGACC TCTCTGCGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu64963
    Clone ID Related Accession (Same CDS sequence) XM_011516929.2 , NM_001362896.2 , NM_001362895.1 , NM_001362896.1 , XM_017013238.1 , XM_017013237.1 , XM_017013234.1 , NM_001362895.2
    Accession Version XM_017013238.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1791bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product poly(U)-binding-splicing factor PUF60 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000008.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGGAGCAGC TGAACTTGGC TGACAGTGGC CTGGTGGGGA AGGAAGCCAG AAGATGCAGA 
    AGGGCCTCCC CTCCCATGGG CATGCCTTCT GCCTGGTGGC TGCAGAATGA GGCCGGCTGC
    CTCTTTGCAG CAAGGCAGGT CAATGGCCAG CAAGGAGGGG GGTCCGAGCC GGCGGCGGCG
    GCGGCAGTGG TGGCAGCGGG AGACAAATGG AAACCTCCAC AGGGCACAGA CTCCATCAAG
    ATGGAGAACG GGCAGAGCAC AGCCGCCAAG CTGGGGCTGC CTCCCCTGAC GCCCGAGCAG
    CAGGAGGCCC TTCAGAAGGC CAAGAAGTAC GCCATGGAGC AGAGCATCAA GAGTGTGCTG
    GTGAAGCAGA CCATCGCGCA CCAGCAGCAG CAGCTCACCA ACCTGCAGAT GGCAGCAGTG
    ACAATGGGCT TTGGAGATCC TCTCTCACCT TTGCAATCGA TGGCGGCTCA GCGGCAGCGG
    GCGCTGGCCA TCATGTGCCG CGTCTACGTG GGCTCTATCT ACTATGAGCT GGGGGAGGAC
    ACCATCCGCC AGGCCTTTGC CCCCTTTGGC CCCATCAAGA GCATCGACAT GTCCTGGGAC
    TCCGTCACCA TGAAGCACAA GGGCTTTGCC TTCGTGGAGT ATGAGGTCCC CGAAGCTGCA
    CAGCTGGCCT TGGAGCAGAT GAACTCGGTG ATGCTGGGGG GCAGGAACAT CAAGGTGGGC
    AGACCCAGCA ACATAGGGCA GGCCCAGCCC ATCATAGACC AGTTGGCTGA GGAGGCACGG
    GCCTTCAACC GCATCTACGT GGCCTCTGTG CACCAGGACC TCTCAGACGA TGACATCAAG
    AGCGTGTTTG AGGCCTTTGG CAAGATCAAG TCCTGCACAC TGGCCCGGGA CCCCACAACT
    GGCAAGCACA AGGGCTACGG CTTCATTGAG TACGAGAAGG CCCAGTCGTC CCAAGATGCT
    GTGTCTTCCA TGAACCTCTT TGACCTGGGT GGCCAGTACT TGCGGGTGGG CAAGGCTGTC
    ACACCGCCCA TGCCCCTACT CACACCAGCC ACGCCTGGAG GCCTCCCACC TGCCGCTGCT
    GTGGCAGCTG CTGCAGCCAC TGCCAAGATC ACAGCTCAGG AAGCAGTGGC CGGAGCAGCG
    GTGCTGGGTA CCCTGGGCAC ACCTGGACTG GTGTCCCCAG CACTGACCCT GGCCCAGCCC
    CTGGGCACTT TGCCCCAGGC TGTCATGGCT GCCCAGGCAC CTGGAGTCAT CACAGGTGTG
    ACCCCAGCCC GTCCTCCTAT CCCGGTCACC ATCCCCTCGG TGGGAGTGGT GAACCCCATC
    CTGGCCAGCC CTCCAACGCT GGGTCTCCTG GAGCCCAAGA AGGAGAAGGA AGAAGAGGAG
    CTGTTTCCCG AGTCAGAGCG GCCAGAGATG CTGAGCGAGC AGGAGCACAT GAGCATCTCG
    GGCAGTAGCG CCCGACACAT GGTGATGCAG AAGCTGCTCC GCAAGCAGGA GTCTACAGTG
    ATGGTTCTGC GCAACATGGT GGACCCCAAG GACATCGATG ATGACCTGGA AGGGGAGGTG
    ACAGAGGAGT GTGGCAAGTT CGGGGCCGTG AACCGCGTCA TCATCTACCA AGAGAAACAA
    GGCGAGGAGG AGGATGCAGA AATCATTGTC AAGATCTTTG TGGAGTTTTC CATAGCCTCT
    GAGACTCATA AGGCCATCCA GGCCCTCAAT GGCCGCTGGT TTGCTGGCCG CAAGGTGGTG
    GCTGAAGTGT ACGACCAGGA GCGTTTTGAT AACAGTGACC TCTCTGCGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016868727.1
    CDS34..1824
    Translation

    Target ORF information:

    RefSeq Version XM_017013238.1
    Organism Homo sapiens(human)
    Definition Homo sapiens poly(U) binding splicing factor 60KDa (PUF60), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017013238.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGGAGCAGC TGAACTTGGC TGACAGTGGC CTGGTGGGGA AGGAAGCCAG AAGATGCAGA 
    AGGGCCTCCC CTCCCATGGG CATGCCTTCT GCCTGGTGGC TGCAGAATGA GGCCGGCTGC
    CTCTTTGCAG CAAGGCAGGT CAATGGCCAG CAAGGAGGGG GGTCCGAGCC GGCGGCGGCG
    GCGGCAGTGG TGGCAGCGGG AGACAAATGG AAACCTCCAC AGGGCACAGA CTCCATCAAG
    ATGGAGAACG GGCAGAGCAC AGCCGCCAAG CTGGGGCTGC CTCCCCTGAC GCCCGAGCAG
    CAGGAGGCCC TTCAGAAGGC CAAGAAGTAC GCCATGGAGC AGAGCATCAA GAGTGTGCTG
    GTGAAGCAGA CCATCGCGCA CCAGCAGCAG CAGCTCACCA ACCTGCAGAT GGCAGCAGTG
    ACAATGGGCT TTGGAGATCC TCTCTCACCT TTGCAATCGA TGGCGGCTCA GCGGCAGCGG
    GCGCTGGCCA TCATGTGCCG CGTCTACGTG GGCTCTATCT ACTATGAGCT GGGGGAGGAC
    ACCATCCGCC AGGCCTTTGC CCCCTTTGGC CCCATCAAGA GCATCGACAT GTCCTGGGAC
    TCCGTCACCA TGAAGCACAA GGGCTTTGCC TTCGTGGAGT ATGAGGTCCC CGAAGCTGCA
    CAGCTGGCCT TGGAGCAGAT GAACTCGGTG ATGCTGGGGG GCAGGAACAT CAAGGTGGGC
    AGACCCAGCA ACATAGGGCA GGCCCAGCCC ATCATAGACC AGTTGGCTGA GGAGGCACGG
    GCCTTCAACC GCATCTACGT GGCCTCTGTG CACCAGGACC TCTCAGACGA TGACATCAAG
    AGCGTGTTTG AGGCCTTTGG CAAGATCAAG TCCTGCACAC TGGCCCGGGA CCCCACAACT
    GGCAAGCACA AGGGCTACGG CTTCATTGAG TACGAGAAGG CCCAGTCGTC CCAAGATGCT
    GTGTCTTCCA TGAACCTCTT TGACCTGGGT GGCCAGTACT TGCGGGTGGG CAAGGCTGTC
    ACACCGCCCA TGCCCCTACT CACACCAGCC ACGCCTGGAG GCCTCCCACC TGCCGCTGCT
    GTGGCAGCTG CTGCAGCCAC TGCCAAGATC ACAGCTCAGG AAGCAGTGGC CGGAGCAGCG
    GTGCTGGGTA CCCTGGGCAC ACCTGGACTG GTGTCCCCAG CACTGACCCT GGCCCAGCCC
    CTGGGCACTT TGCCCCAGGC TGTCATGGCT GCCCAGGCAC CTGGAGTCAT CACAGGTGTG
    ACCCCAGCCC GTCCTCCTAT CCCGGTCACC ATCCCCTCGG TGGGAGTGGT GAACCCCATC
    CTGGCCAGCC CTCCAACGCT GGGTCTCCTG GAGCCCAAGA AGGAGAAGGA AGAAGAGGAG
    CTGTTTCCCG AGTCAGAGCG GCCAGAGATG CTGAGCGAGC AGGAGCACAT GAGCATCTCG
    GGCAGTAGCG CCCGACACAT GGTGATGCAG AAGCTGCTCC GCAAGCAGGA GTCTACAGTG
    ATGGTTCTGC GCAACATGGT GGACCCCAAG GACATCGATG ATGACCTGGA AGGGGAGGTG
    ACAGAGGAGT GTGGCAAGTT CGGGGCCGTG AACCGCGTCA TCATCTACCA AGAGAAACAA
    GGCGAGGAGG AGGATGCAGA AATCATTGTC AAGATCTTTG TGGAGTTTTC CATAGCCTCT
    GAGACTCATA AGGCCATCCA GGCCCTCAAT GGCCGCTGGT TTGCTGGCCG CAAGGTGGTG
    GCTGAAGTGT ACGACCAGGA GCGTTTTGAT AACAGTGACC TCTCTGCGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu64963
    Clone ID Related Accession (Same CDS sequence) XM_011516929.2 , NM_001362896.2 , NM_001362895.1 , NM_001362896.1 , XM_017013238.1 , XM_017013237.1 , XM_017013234.1 , NM_001362895.2
    Accession Version XM_017013237.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1791bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product poly(U)-binding-splicing factor PUF60 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000008.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGGAGCAGC TGAACTTGGC TGACAGTGGC CTGGTGGGGA AGGAAGCCAG AAGATGCAGA 
    AGGGCCTCCC CTCCCATGGG CATGCCTTCT GCCTGGTGGC TGCAGAATGA GGCCGGCTGC
    CTCTTTGCAG CAAGGCAGGT CAATGGCCAG CAAGGAGGGG GGTCCGAGCC GGCGGCGGCG
    GCGGCAGTGG TGGCAGCGGG AGACAAATGG AAACCTCCAC AGGGCACAGA CTCCATCAAG
    ATGGAGAACG GGCAGAGCAC AGCCGCCAAG CTGGGGCTGC CTCCCCTGAC GCCCGAGCAG
    CAGGAGGCCC TTCAGAAGGC CAAGAAGTAC GCCATGGAGC AGAGCATCAA GAGTGTGCTG
    GTGAAGCAGA CCATCGCGCA CCAGCAGCAG CAGCTCACCA ACCTGCAGAT GGCAGCAGTG
    ACAATGGGCT TTGGAGATCC TCTCTCACCT TTGCAATCGA TGGCGGCTCA GCGGCAGCGG
    GCGCTGGCCA TCATGTGCCG CGTCTACGTG GGCTCTATCT ACTATGAGCT GGGGGAGGAC
    ACCATCCGCC AGGCCTTTGC CCCCTTTGGC CCCATCAAGA GCATCGACAT GTCCTGGGAC
    TCCGTCACCA TGAAGCACAA GGGCTTTGCC TTCGTGGAGT ATGAGGTCCC CGAAGCTGCA
    CAGCTGGCCT TGGAGCAGAT GAACTCGGTG ATGCTGGGGG GCAGGAACAT CAAGGTGGGC
    AGACCCAGCA ACATAGGGCA GGCCCAGCCC ATCATAGACC AGTTGGCTGA GGAGGCACGG
    GCCTTCAACC GCATCTACGT GGCCTCTGTG CACCAGGACC TCTCAGACGA TGACATCAAG
    AGCGTGTTTG AGGCCTTTGG CAAGATCAAG TCCTGCACAC TGGCCCGGGA CCCCACAACT
    GGCAAGCACA AGGGCTACGG CTTCATTGAG TACGAGAAGG CCCAGTCGTC CCAAGATGCT
    GTGTCTTCCA TGAACCTCTT TGACCTGGGT GGCCAGTACT TGCGGGTGGG CAAGGCTGTC
    ACACCGCCCA TGCCCCTACT CACACCAGCC ACGCCTGGAG GCCTCCCACC TGCCGCTGCT
    GTGGCAGCTG CTGCAGCCAC TGCCAAGATC ACAGCTCAGG AAGCAGTGGC CGGAGCAGCG
    GTGCTGGGTA CCCTGGGCAC ACCTGGACTG GTGTCCCCAG CACTGACCCT GGCCCAGCCC
    CTGGGCACTT TGCCCCAGGC TGTCATGGCT GCCCAGGCAC CTGGAGTCAT CACAGGTGTG
    ACCCCAGCCC GTCCTCCTAT CCCGGTCACC ATCCCCTCGG TGGGAGTGGT GAACCCCATC
    CTGGCCAGCC CTCCAACGCT GGGTCTCCTG GAGCCCAAGA AGGAGAAGGA AGAAGAGGAG
    CTGTTTCCCG AGTCAGAGCG GCCAGAGATG CTGAGCGAGC AGGAGCACAT GAGCATCTCG
    GGCAGTAGCG CCCGACACAT GGTGATGCAG AAGCTGCTCC GCAAGCAGGA GTCTACAGTG
    ATGGTTCTGC GCAACATGGT GGACCCCAAG GACATCGATG ATGACCTGGA AGGGGAGGTG
    ACAGAGGAGT GTGGCAAGTT CGGGGCCGTG AACCGCGTCA TCATCTACCA AGAGAAACAA
    GGCGAGGAGG AGGATGCAGA AATCATTGTC AAGATCTTTG TGGAGTTTTC CATAGCCTCT
    GAGACTCATA AGGCCATCCA GGCCCTCAAT GGCCGCTGGT TTGCTGGCCG CAAGGTGGTG
    GCTGAAGTGT ACGACCAGGA GCGTTTTGAT AACAGTGACC TCTCTGCGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016868726.1
    CDS234..2024
    Translation

    Target ORF information:

    RefSeq Version XM_017013237.1
    Organism Homo sapiens(human)
    Definition Homo sapiens poly(U) binding splicing factor 60KDa (PUF60), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017013237.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGGAGCAGC TGAACTTGGC TGACAGTGGC CTGGTGGGGA AGGAAGCCAG AAGATGCAGA 
    AGGGCCTCCC CTCCCATGGG CATGCCTTCT GCCTGGTGGC TGCAGAATGA GGCCGGCTGC
    CTCTTTGCAG CAAGGCAGGT CAATGGCCAG CAAGGAGGGG GGTCCGAGCC GGCGGCGGCG
    GCGGCAGTGG TGGCAGCGGG AGACAAATGG AAACCTCCAC AGGGCACAGA CTCCATCAAG
    ATGGAGAACG GGCAGAGCAC AGCCGCCAAG CTGGGGCTGC CTCCCCTGAC GCCCGAGCAG
    CAGGAGGCCC TTCAGAAGGC CAAGAAGTAC GCCATGGAGC AGAGCATCAA GAGTGTGCTG
    GTGAAGCAGA CCATCGCGCA CCAGCAGCAG CAGCTCACCA ACCTGCAGAT GGCAGCAGTG
    ACAATGGGCT TTGGAGATCC TCTCTCACCT TTGCAATCGA TGGCGGCTCA GCGGCAGCGG
    GCGCTGGCCA TCATGTGCCG CGTCTACGTG GGCTCTATCT ACTATGAGCT GGGGGAGGAC
    ACCATCCGCC AGGCCTTTGC CCCCTTTGGC CCCATCAAGA GCATCGACAT GTCCTGGGAC
    TCCGTCACCA TGAAGCACAA GGGCTTTGCC TTCGTGGAGT ATGAGGTCCC CGAAGCTGCA
    CAGCTGGCCT TGGAGCAGAT GAACTCGGTG ATGCTGGGGG GCAGGAACAT CAAGGTGGGC
    AGACCCAGCA ACATAGGGCA GGCCCAGCCC ATCATAGACC AGTTGGCTGA GGAGGCACGG
    GCCTTCAACC GCATCTACGT GGCCTCTGTG CACCAGGACC TCTCAGACGA TGACATCAAG
    AGCGTGTTTG AGGCCTTTGG CAAGATCAAG TCCTGCACAC TGGCCCGGGA CCCCACAACT
    GGCAAGCACA AGGGCTACGG CTTCATTGAG TACGAGAAGG CCCAGTCGTC CCAAGATGCT
    GTGTCTTCCA TGAACCTCTT TGACCTGGGT GGCCAGTACT TGCGGGTGGG CAAGGCTGTC
    ACACCGCCCA TGCCCCTACT CACACCAGCC ACGCCTGGAG GCCTCCCACC TGCCGCTGCT
    GTGGCAGCTG CTGCAGCCAC TGCCAAGATC ACAGCTCAGG AAGCAGTGGC CGGAGCAGCG
    GTGCTGGGTA CCCTGGGCAC ACCTGGACTG GTGTCCCCAG CACTGACCCT GGCCCAGCCC
    CTGGGCACTT TGCCCCAGGC TGTCATGGCT GCCCAGGCAC CTGGAGTCAT CACAGGTGTG
    ACCCCAGCCC GTCCTCCTAT CCCGGTCACC ATCCCCTCGG TGGGAGTGGT GAACCCCATC
    CTGGCCAGCC CTCCAACGCT GGGTCTCCTG GAGCCCAAGA AGGAGAAGGA AGAAGAGGAG
    CTGTTTCCCG AGTCAGAGCG GCCAGAGATG CTGAGCGAGC AGGAGCACAT GAGCATCTCG
    GGCAGTAGCG CCCGACACAT GGTGATGCAG AAGCTGCTCC GCAAGCAGGA GTCTACAGTG
    ATGGTTCTGC GCAACATGGT GGACCCCAAG GACATCGATG ATGACCTGGA AGGGGAGGTG
    ACAGAGGAGT GTGGCAAGTT CGGGGCCGTG AACCGCGTCA TCATCTACCA AGAGAAACAA
    GGCGAGGAGG AGGATGCAGA AATCATTGTC AAGATCTTTG TGGAGTTTTC CATAGCCTCT
    GAGACTCATA AGGCCATCCA GGCCCTCAAT GGCCGCTGGT TTGCTGGCCG CAAGGTGGTG
    GCTGAAGTGT ACGACCAGGA GCGTTTTGAT AACAGTGACC TCTCTGCGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu64963
    Clone ID Related Accession (Same CDS sequence) XM_011516929.2 , NM_001362896.2 , NM_001362895.1 , NM_001362896.1 , XM_017013238.1 , XM_017013237.1 , XM_017013234.1 , NM_001362895.2
    Accession Version XM_017013234.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1791bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product poly(U)-binding-splicing factor PUF60 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000008.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGGAGCAGC TGAACTTGGC TGACAGTGGC CTGGTGGGGA AGGAAGCCAG AAGATGCAGA 
    AGGGCCTCCC CTCCCATGGG CATGCCTTCT GCCTGGTGGC TGCAGAATGA GGCCGGCTGC
    CTCTTTGCAG CAAGGCAGGT CAATGGCCAG CAAGGAGGGG GGTCCGAGCC GGCGGCGGCG
    GCGGCAGTGG TGGCAGCGGG AGACAAATGG AAACCTCCAC AGGGCACAGA CTCCATCAAG
    ATGGAGAACG GGCAGAGCAC AGCCGCCAAG CTGGGGCTGC CTCCCCTGAC GCCCGAGCAG
    CAGGAGGCCC TTCAGAAGGC CAAGAAGTAC GCCATGGAGC AGAGCATCAA GAGTGTGCTG
    GTGAAGCAGA CCATCGCGCA CCAGCAGCAG CAGCTCACCA ACCTGCAGAT GGCAGCAGTG
    ACAATGGGCT TTGGAGATCC TCTCTCACCT TTGCAATCGA TGGCGGCTCA GCGGCAGCGG
    GCGCTGGCCA TCATGTGCCG CGTCTACGTG GGCTCTATCT ACTATGAGCT GGGGGAGGAC
    ACCATCCGCC AGGCCTTTGC CCCCTTTGGC CCCATCAAGA GCATCGACAT GTCCTGGGAC
    TCCGTCACCA TGAAGCACAA GGGCTTTGCC TTCGTGGAGT ATGAGGTCCC CGAAGCTGCA
    CAGCTGGCCT TGGAGCAGAT GAACTCGGTG ATGCTGGGGG GCAGGAACAT CAAGGTGGGC
    AGACCCAGCA ACATAGGGCA GGCCCAGCCC ATCATAGACC AGTTGGCTGA GGAGGCACGG
    GCCTTCAACC GCATCTACGT GGCCTCTGTG CACCAGGACC TCTCAGACGA TGACATCAAG
    AGCGTGTTTG AGGCCTTTGG CAAGATCAAG TCCTGCACAC TGGCCCGGGA CCCCACAACT
    GGCAAGCACA AGGGCTACGG CTTCATTGAG TACGAGAAGG CCCAGTCGTC CCAAGATGCT
    GTGTCTTCCA TGAACCTCTT TGACCTGGGT GGCCAGTACT TGCGGGTGGG CAAGGCTGTC
    ACACCGCCCA TGCCCCTACT CACACCAGCC ACGCCTGGAG GCCTCCCACC TGCCGCTGCT
    GTGGCAGCTG CTGCAGCCAC TGCCAAGATC ACAGCTCAGG AAGCAGTGGC CGGAGCAGCG
    GTGCTGGGTA CCCTGGGCAC ACCTGGACTG GTGTCCCCAG CACTGACCCT GGCCCAGCCC
    CTGGGCACTT TGCCCCAGGC TGTCATGGCT GCCCAGGCAC CTGGAGTCAT CACAGGTGTG
    ACCCCAGCCC GTCCTCCTAT CCCGGTCACC ATCCCCTCGG TGGGAGTGGT GAACCCCATC
    CTGGCCAGCC CTCCAACGCT GGGTCTCCTG GAGCCCAAGA AGGAGAAGGA AGAAGAGGAG
    CTGTTTCCCG AGTCAGAGCG GCCAGAGATG CTGAGCGAGC AGGAGCACAT GAGCATCTCG
    GGCAGTAGCG CCCGACACAT GGTGATGCAG AAGCTGCTCC GCAAGCAGGA GTCTACAGTG
    ATGGTTCTGC GCAACATGGT GGACCCCAAG GACATCGATG ATGACCTGGA AGGGGAGGTG
    ACAGAGGAGT GTGGCAAGTT CGGGGCCGTG AACCGCGTCA TCATCTACCA AGAGAAACAA
    GGCGAGGAGG AGGATGCAGA AATCATTGTC AAGATCTTTG TGGAGTTTTC CATAGCCTCT
    GAGACTCATA AGGCCATCCA GGCCCTCAAT GGCCGCTGGT TTGCTGGCCG CAAGGTGGTG
    GCTGAAGTGT ACGACCAGGA GCGTTTTGAT AACAGTGACC TCTCTGCGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016868723.1
    CDS1505..3295
    Translation

    Target ORF information:

    RefSeq Version XM_017013234.1
    Organism Homo sapiens(human)
    Definition Homo sapiens poly(U) binding splicing factor 60 (PUF60), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017013234.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGGAGCAGC TGAACTTGGC TGACAGTGGC CTGGTGGGGA AGGAAGCCAG AAGATGCAGA 
    AGGGCCTCCC CTCCCATGGG CATGCCTTCT GCCTGGTGGC TGCAGAATGA GGCCGGCTGC
    CTCTTTGCAG CAAGGCAGGT CAATGGCCAG CAAGGAGGGG GGTCCGAGCC GGCGGCGGCG
    GCGGCAGTGG TGGCAGCGGG AGACAAATGG AAACCTCCAC AGGGCACAGA CTCCATCAAG
    ATGGAGAACG GGCAGAGCAC AGCCGCCAAG CTGGGGCTGC CTCCCCTGAC GCCCGAGCAG
    CAGGAGGCCC TTCAGAAGGC CAAGAAGTAC GCCATGGAGC AGAGCATCAA GAGTGTGCTG
    GTGAAGCAGA CCATCGCGCA CCAGCAGCAG CAGCTCACCA ACCTGCAGAT GGCAGCAGTG
    ACAATGGGCT TTGGAGATCC TCTCTCACCT TTGCAATCGA TGGCGGCTCA GCGGCAGCGG
    GCGCTGGCCA TCATGTGCCG CGTCTACGTG GGCTCTATCT ACTATGAGCT GGGGGAGGAC
    ACCATCCGCC AGGCCTTTGC CCCCTTTGGC CCCATCAAGA GCATCGACAT GTCCTGGGAC
    TCCGTCACCA TGAAGCACAA GGGCTTTGCC TTCGTGGAGT ATGAGGTCCC CGAAGCTGCA
    CAGCTGGCCT TGGAGCAGAT GAACTCGGTG ATGCTGGGGG GCAGGAACAT CAAGGTGGGC
    AGACCCAGCA ACATAGGGCA GGCCCAGCCC ATCATAGACC AGTTGGCTGA GGAGGCACGG
    GCCTTCAACC GCATCTACGT GGCCTCTGTG CACCAGGACC TCTCAGACGA TGACATCAAG
    AGCGTGTTTG AGGCCTTTGG CAAGATCAAG TCCTGCACAC TGGCCCGGGA CCCCACAACT
    GGCAAGCACA AGGGCTACGG CTTCATTGAG TACGAGAAGG CCCAGTCGTC CCAAGATGCT
    GTGTCTTCCA TGAACCTCTT TGACCTGGGT GGCCAGTACT TGCGGGTGGG CAAGGCTGTC
    ACACCGCCCA TGCCCCTACT CACACCAGCC ACGCCTGGAG GCCTCCCACC TGCCGCTGCT
    GTGGCAGCTG CTGCAGCCAC TGCCAAGATC ACAGCTCAGG AAGCAGTGGC CGGAGCAGCG
    GTGCTGGGTA CCCTGGGCAC ACCTGGACTG GTGTCCCCAG CACTGACCCT GGCCCAGCCC
    CTGGGCACTT TGCCCCAGGC TGTCATGGCT GCCCAGGCAC CTGGAGTCAT CACAGGTGTG
    ACCCCAGCCC GTCCTCCTAT CCCGGTCACC ATCCCCTCGG TGGGAGTGGT GAACCCCATC
    CTGGCCAGCC CTCCAACGCT GGGTCTCCTG GAGCCCAAGA AGGAGAAGGA AGAAGAGGAG
    CTGTTTCCCG AGTCAGAGCG GCCAGAGATG CTGAGCGAGC AGGAGCACAT GAGCATCTCG
    GGCAGTAGCG CCCGACACAT GGTGATGCAG AAGCTGCTCC GCAAGCAGGA GTCTACAGTG
    ATGGTTCTGC GCAACATGGT GGACCCCAAG GACATCGATG ATGACCTGGA AGGGGAGGTG
    ACAGAGGAGT GTGGCAAGTT CGGGGCCGTG AACCGCGTCA TCATCTACCA AGAGAAACAA
    GGCGAGGAGG AGGATGCAGA AATCATTGTC AAGATCTTTG TGGAGTTTTC CATAGCCTCT
    GAGACTCATA AGGCCATCCA GGCCCTCAAT GGCCGCTGGT TTGCTGGCCG CAAGGTGGTG
    GCTGAAGTGT ACGACCAGGA GCGTTTTGAT AACAGTGACC TCTCTGCGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu64964
    Clone ID Related Accession (Same CDS sequence) NM_001362897.1 , XM_017013236.1 , NM_001362897.2 , XM_011516930.2
    Accession Version XM_017013236.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1740bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product poly(U)-binding-splicing factor PUF60 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000008.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGGAGCAGC TGAACTTGGC TGACAGTGGC CTGGTGGGGA AGGAAGCCAG AAGATGCAGA 
    AGGGCCTCCC CTCCCATGGG CATGCCTTCT GCCTGGTGGC TGCAGAATGA GGCCGGCTGC
    CTCTTTGCAG CAAGGCAGGT CAATGGCCAG CAAGGAGGGG GGTCCGAGCC GGCGGCGGCG
    GCGGCAGTGG TGGCAGCGGG AGACAAATGG AAACCTCCAC AGGGCACAGA CTCCATCAAG
    ATGGAGAACG GGCAGAGCAC AGCCGCCAAG CTGGGGCTGC CTCCCCTGAC GCCCGAGCAG
    CAGGAGGCCC TTCAGAAGGC CAAGAAGTAC GCCATGGAGC AGAGCATCAA GAGTGTGCTG
    GTGAAGCAGA CCATCGCGCA CCAGCAGCAG CAGCTCACCA ACCTGCAGAT GGCGGCTCAG
    CGGCAGCGGG CGCTGGCCAT CATGTGCCGC GTCTACGTGG GCTCTATCTA CTATGAGCTG
    GGGGAGGACA CCATCCGCCA GGCCTTTGCC CCCTTTGGCC CCATCAAGAG CATCGACATG
    TCCTGGGACT CCGTCACCAT GAAGCACAAG GGCTTTGCCT TCGTGGAGTA TGAGGTCCCC
    GAAGCTGCAC AGCTGGCCTT GGAGCAGATG AACTCGGTGA TGCTGGGGGG CAGGAACATC
    AAGGTGGGCA GACCCAGCAA CATAGGGCAG GCCCAGCCCA TCATAGACCA GTTGGCTGAG
    GAGGCACGGG CCTTCAACCG CATCTACGTG GCCTCTGTGC ACCAGGACCT CTCAGACGAT
    GACATCAAGA GCGTGTTTGA GGCCTTTGGC AAGATCAAGT CCTGCACACT GGCCCGGGAC
    CCCACAACTG GCAAGCACAA GGGCTACGGC TTCATTGAGT ACGAGAAGGC CCAGTCGTCC
    CAAGATGCTG TGTCTTCCAT GAACCTCTTT GACCTGGGTG GCCAGTACTT GCGGGTGGGC
    AAGGCTGTCA CACCGCCCAT GCCCCTACTC ACACCAGCCA CGCCTGGAGG CCTCCCACCT
    GCCGCTGCTG TGGCAGCTGC TGCAGCCACT GCCAAGATCA CAGCTCAGGA AGCAGTGGCC
    GGAGCAGCGG TGCTGGGTAC CCTGGGCACA CCTGGACTGG TGTCCCCAGC ACTGACCCTG
    GCCCAGCCCC TGGGCACTTT GCCCCAGGCT GTCATGGCTG CCCAGGCACC TGGAGTCATC
    ACAGGTGTGA CCCCAGCCCG TCCTCCTATC CCGGTCACCA TCCCCTCGGT GGGAGTGGTG
    AACCCCATCC TGGCCAGCCC TCCAACGCTG GGTCTCCTGG AGCCCAAGAA GGAGAAGGAA
    GAAGAGGAGC TGTTTCCCGA GTCAGAGCGG CCAGAGATGC TGAGCGAGCA GGAGCACATG
    AGCATCTCGG GCAGTAGCGC CCGACACATG GTGATGCAGA AGCTGCTCCG CAAGCAGGAG
    TCTACAGTGA TGGTTCTGCG CAACATGGTG GACCCCAAGG ACATCGATGA TGACCTGGAA
    GGGGAGGTGA CAGAGGAGTG TGGCAAGTTC GGGGCCGTGA ACCGCGTCAT CATCTACCAA
    GAGAAACAAG GCGAGGAGGA GGATGCAGAA ATCATTGTCA AGATCTTTGT GGAGTTTTCC
    ATAGCCTCTG AGACTCATAA GGCCATCCAG GCCCTCAATG GCCGCTGGTT TGCTGGCCGC
    AAGGTGGTGG CTGAAGTGTA CGACCAGGAG CGTTTTGATA ACAGTGACCT CTCTGCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016868725.1
    CDS1505..3244
    Translation

    Target ORF information:

    RefSeq Version XM_017013236.1
    Organism Homo sapiens(human)
    Definition Homo sapiens poly(U) binding splicing factor 60 (PUF60), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017013236.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGGAGCAGC TGAACTTGGC TGACAGTGGC CTGGTGGGGA AGGAAGCCAG AAGATGCAGA 
    AGGGCCTCCC CTCCCATGGG CATGCCTTCT GCCTGGTGGC TGCAGAATGA GGCCGGCTGC
    CTCTTTGCAG CAAGGCAGGT CAATGGCCAG CAAGGAGGGG GGTCCGAGCC GGCGGCGGCG
    GCGGCAGTGG TGGCAGCGGG AGACAAATGG AAACCTCCAC AGGGCACAGA CTCCATCAAG
    ATGGAGAACG GGCAGAGCAC AGCCGCCAAG CTGGGGCTGC CTCCCCTGAC GCCCGAGCAG
    CAGGAGGCCC TTCAGAAGGC CAAGAAGTAC GCCATGGAGC AGAGCATCAA GAGTGTGCTG
    GTGAAGCAGA CCATCGCGCA CCAGCAGCAG CAGCTCACCA ACCTGCAGAT GGCGGCTCAG
    CGGCAGCGGG CGCTGGCCAT CATGTGCCGC GTCTACGTGG GCTCTATCTA CTATGAGCTG
    GGGGAGGACA CCATCCGCCA GGCCTTTGCC CCCTTTGGCC CCATCAAGAG CATCGACATG
    TCCTGGGACT CCGTCACCAT GAAGCACAAG GGCTTTGCCT TCGTGGAGTA TGAGGTCCCC
    GAAGCTGCAC AGCTGGCCTT GGAGCAGATG AACTCGGTGA TGCTGGGGGG CAGGAACATC
    AAGGTGGGCA GACCCAGCAA CATAGGGCAG GCCCAGCCCA TCATAGACCA GTTGGCTGAG
    GAGGCACGGG CCTTCAACCG CATCTACGTG GCCTCTGTGC ACCAGGACCT CTCAGACGAT
    GACATCAAGA GCGTGTTTGA GGCCTTTGGC AAGATCAAGT CCTGCACACT GGCCCGGGAC
    CCCACAACTG GCAAGCACAA GGGCTACGGC TTCATTGAGT ACGAGAAGGC CCAGTCGTCC
    CAAGATGCTG TGTCTTCCAT GAACCTCTTT GACCTGGGTG GCCAGTACTT GCGGGTGGGC
    AAGGCTGTCA CACCGCCCAT GCCCCTACTC ACACCAGCCA CGCCTGGAGG CCTCCCACCT
    GCCGCTGCTG TGGCAGCTGC TGCAGCCACT GCCAAGATCA CAGCTCAGGA AGCAGTGGCC
    GGAGCAGCGG TGCTGGGTAC CCTGGGCACA CCTGGACTGG TGTCCCCAGC ACTGACCCTG
    GCCCAGCCCC TGGGCACTTT GCCCCAGGCT GTCATGGCTG CCCAGGCACC TGGAGTCATC
    ACAGGTGTGA CCCCAGCCCG TCCTCCTATC CCGGTCACCA TCCCCTCGGT GGGAGTGGTG
    AACCCCATCC TGGCCAGCCC TCCAACGCTG GGTCTCCTGG AGCCCAAGAA GGAGAAGGAA
    GAAGAGGAGC TGTTTCCCGA GTCAGAGCGG CCAGAGATGC TGAGCGAGCA GGAGCACATG
    AGCATCTCGG GCAGTAGCGC CCGACACATG GTGATGCAGA AGCTGCTCCG CAAGCAGGAG
    TCTACAGTGA TGGTTCTGCG CAACATGGTG GACCCCAAGG ACATCGATGA TGACCTGGAA
    GGGGAGGTGA CAGAGGAGTG TGGCAAGTTC GGGGCCGTGA ACCGCGTCAT CATCTACCAA
    GAGAAACAAG GCGAGGAGGA GGATGCAGAA ATCATTGTCA AGATCTTTGT GGAGTTTTCC
    ATAGCCTCTG AGACTCATAA GGCCATCCAG GCCCTCAATG GCCGCTGGTT TGCTGGCCGC
    AAGGTGGTGG CTGAAGTGTA CGACCAGGAG CGTTTTGATA ACAGTGACCT CTCTGCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu64964
    Clone ID Related Accession (Same CDS sequence) NM_001362897.1 , XM_017013236.1 , NM_001362897.2 , XM_011516930.2
    Accession Version XM_011516930.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1740bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product poly(U)-binding-splicing factor PUF60 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000008.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011516930.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGGAGCAGC TGAACTTGGC TGACAGTGGC CTGGTGGGGA AGGAAGCCAG AAGATGCAGA 
    AGGGCCTCCC CTCCCATGGG CATGCCTTCT GCCTGGTGGC TGCAGAATGA GGCCGGCTGC
    CTCTTTGCAG CAAGGCAGGT CAATGGCCAG CAAGGAGGGG GGTCCGAGCC GGCGGCGGCG
    GCGGCAGTGG TGGCAGCGGG AGACAAATGG AAACCTCCAC AGGGCACAGA CTCCATCAAG
    ATGGAGAACG GGCAGAGCAC AGCCGCCAAG CTGGGGCTGC CTCCCCTGAC GCCCGAGCAG
    CAGGAGGCCC TTCAGAAGGC CAAGAAGTAC GCCATGGAGC AGAGCATCAA GAGTGTGCTG
    GTGAAGCAGA CCATCGCGCA CCAGCAGCAG CAGCTCACCA ACCTGCAGAT GGCGGCTCAG
    CGGCAGCGGG CGCTGGCCAT CATGTGCCGC GTCTACGTGG GCTCTATCTA CTATGAGCTG
    GGGGAGGACA CCATCCGCCA GGCCTTTGCC CCCTTTGGCC CCATCAAGAG CATCGACATG
    TCCTGGGACT CCGTCACCAT GAAGCACAAG GGCTTTGCCT TCGTGGAGTA TGAGGTCCCC
    GAAGCTGCAC AGCTGGCCTT GGAGCAGATG AACTCGGTGA TGCTGGGGGG CAGGAACATC
    AAGGTGGGCA GACCCAGCAA CATAGGGCAG GCCCAGCCCA TCATAGACCA GTTGGCTGAG
    GAGGCACGGG CCTTCAACCG CATCTACGTG GCCTCTGTGC ACCAGGACCT CTCAGACGAT
    GACATCAAGA GCGTGTTTGA GGCCTTTGGC AAGATCAAGT CCTGCACACT GGCCCGGGAC
    CCCACAACTG GCAAGCACAA GGGCTACGGC TTCATTGAGT ACGAGAAGGC CCAGTCGTCC
    CAAGATGCTG TGTCTTCCAT GAACCTCTTT GACCTGGGTG GCCAGTACTT GCGGGTGGGC
    AAGGCTGTCA CACCGCCCAT GCCCCTACTC ACACCAGCCA CGCCTGGAGG CCTCCCACCT
    GCCGCTGCTG TGGCAGCTGC TGCAGCCACT GCCAAGATCA CAGCTCAGGA AGCAGTGGCC
    GGAGCAGCGG TGCTGGGTAC CCTGGGCACA CCTGGACTGG TGTCCCCAGC ACTGACCCTG
    GCCCAGCCCC TGGGCACTTT GCCCCAGGCT GTCATGGCTG CCCAGGCACC TGGAGTCATC
    ACAGGTGTGA CCCCAGCCCG TCCTCCTATC CCGGTCACCA TCCCCTCGGT GGGAGTGGTG
    AACCCCATCC TGGCCAGCCC TCCAACGCTG GGTCTCCTGG AGCCCAAGAA GGAGAAGGAA
    GAAGAGGAGC TGTTTCCCGA GTCAGAGCGG CCAGAGATGC TGAGCGAGCA GGAGCACATG
    AGCATCTCGG GCAGTAGCGC CCGACACATG GTGATGCAGA AGCTGCTCCG CAAGCAGGAG
    TCTACAGTGA TGGTTCTGCG CAACATGGTG GACCCCAAGG ACATCGATGA TGACCTGGAA
    GGGGAGGTGA CAGAGGAGTG TGGCAAGTTC GGGGCCGTGA ACCGCGTCAT CATCTACCAA
    GAGAAACAAG GCGAGGAGGA GGATGCAGAA ATCATTGTCA AGATCTTTGT GGAGTTTTCC
    ATAGCCTCTG AGACTCATAA GGCCATCCAG GCCCTCAATG GCCGCTGGTT TGCTGGCCGC
    AAGGTGGTGG CTGAAGTGTA CGACCAGGAG CGTTTTGATA ACAGTGACCT CTCTGCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011515232.1
    CDS74..1813
    Translation

    Target ORF information:

    RefSeq Version XM_011516930.2
    Organism Homo sapiens(human)
    Definition Homo sapiens poly(U) binding splicing factor 60KDa (PUF60), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011516930.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGGAGCAGC TGAACTTGGC TGACAGTGGC CTGGTGGGGA AGGAAGCCAG AAGATGCAGA 
    AGGGCCTCCC CTCCCATGGG CATGCCTTCT GCCTGGTGGC TGCAGAATGA GGCCGGCTGC
    CTCTTTGCAG CAAGGCAGGT CAATGGCCAG CAAGGAGGGG GGTCCGAGCC GGCGGCGGCG
    GCGGCAGTGG TGGCAGCGGG AGACAAATGG AAACCTCCAC AGGGCACAGA CTCCATCAAG
    ATGGAGAACG GGCAGAGCAC AGCCGCCAAG CTGGGGCTGC CTCCCCTGAC GCCCGAGCAG
    CAGGAGGCCC TTCAGAAGGC CAAGAAGTAC GCCATGGAGC AGAGCATCAA GAGTGTGCTG
    GTGAAGCAGA CCATCGCGCA CCAGCAGCAG CAGCTCACCA ACCTGCAGAT GGCGGCTCAG
    CGGCAGCGGG CGCTGGCCAT CATGTGCCGC GTCTACGTGG GCTCTATCTA CTATGAGCTG
    GGGGAGGACA CCATCCGCCA GGCCTTTGCC CCCTTTGGCC CCATCAAGAG CATCGACATG
    TCCTGGGACT CCGTCACCAT GAAGCACAAG GGCTTTGCCT TCGTGGAGTA TGAGGTCCCC
    GAAGCTGCAC AGCTGGCCTT GGAGCAGATG AACTCGGTGA TGCTGGGGGG CAGGAACATC
    AAGGTGGGCA GACCCAGCAA CATAGGGCAG GCCCAGCCCA TCATAGACCA GTTGGCTGAG
    GAGGCACGGG CCTTCAACCG CATCTACGTG GCCTCTGTGC ACCAGGACCT CTCAGACGAT
    GACATCAAGA GCGTGTTTGA GGCCTTTGGC AAGATCAAGT CCTGCACACT GGCCCGGGAC
    CCCACAACTG GCAAGCACAA GGGCTACGGC TTCATTGAGT ACGAGAAGGC CCAGTCGTCC
    CAAGATGCTG TGTCTTCCAT GAACCTCTTT GACCTGGGTG GCCAGTACTT GCGGGTGGGC
    AAGGCTGTCA CACCGCCCAT GCCCCTACTC ACACCAGCCA CGCCTGGAGG CCTCCCACCT
    GCCGCTGCTG TGGCAGCTGC TGCAGCCACT GCCAAGATCA CAGCTCAGGA AGCAGTGGCC
    GGAGCAGCGG TGCTGGGTAC CCTGGGCACA CCTGGACTGG TGTCCCCAGC ACTGACCCTG
    GCCCAGCCCC TGGGCACTTT GCCCCAGGCT GTCATGGCTG CCCAGGCACC TGGAGTCATC
    ACAGGTGTGA CCCCAGCCCG TCCTCCTATC CCGGTCACCA TCCCCTCGGT GGGAGTGGTG
    AACCCCATCC TGGCCAGCCC TCCAACGCTG GGTCTCCTGG AGCCCAAGAA GGAGAAGGAA
    GAAGAGGAGC TGTTTCCCGA GTCAGAGCGG CCAGAGATGC TGAGCGAGCA GGAGCACATG
    AGCATCTCGG GCAGTAGCGC CCGACACATG GTGATGCAGA AGCTGCTCCG CAAGCAGGAG
    TCTACAGTGA TGGTTCTGCG CAACATGGTG GACCCCAAGG ACATCGATGA TGACCTGGAA
    GGGGAGGTGA CAGAGGAGTG TGGCAAGTTC GGGGCCGTGA ACCGCGTCAT CATCTACCAA
    GAGAAACAAG GCGAGGAGGA GGATGCAGAA ATCATTGTCA AGATCTTTGT GGAGTTTTCC
    ATAGCCTCTG AGACTCATAA GGCCATCCAG GCCCTCAATG GCCGCTGGTT TGCTGGCCGC
    AAGGTGGTGG CTGAAGTGTA CGACCAGGAG CGTTTTGATA ACAGTGACCT CTCTGCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu97848
    Clone ID Related Accession (Same CDS sequence) XM_017013235.1
    Accession Version XM_017013235.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1755bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product poly(U)-binding-splicing factor PUF60 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000008.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGCTGCTTA AAAGTGTCTT CAGGAGAGAA CTGGGACCTG GGCTGGGCCA GAGCCTGTGG 
    AAGGCACTGG AGCTGGGGCT GAGTTGTGGG CTGGCCAAGC AGGTCAATGG CCAGCAAGGA
    GGGGGGTCCG AGCCGGCGGC GGCGGCGGCA GTGGTGGCAG CGGGAGACAA ATGGAAACCT
    CCACAGGGCA CAGACTCCAT CAAGATGGAG AACGGGCAGA GCACAGCCGC CAAGCTGGGG
    CTGCCTCCCC TGACGCCCGA GCAGCAGGAG GCCCTTCAGA AGGCCAAGAA GTACGCCATG
    GAGCAGAGCA TCAAGAGTGT GCTGGTGAAG CAGACCATCG CGCACCAGCA GCAGCAGCTC
    ACCAACCTGC AGATGGCAGC AGTGACAATG GGCTTTGGAG ATCCTCTCTC ACCTTTGCAA
    TCGATGGCGG CTCAGCGGCA GCGGGCGCTG GCCATCATGT GCCGCGTCTA CGTGGGCTCT
    ATCTACTATG AGCTGGGGGA GGACACCATC CGCCAGGCCT TTGCCCCCTT TGGCCCCATC
    AAGAGCATCG ACATGTCCTG GGACTCCGTC ACCATGAAGC ACAAGGGCTT TGCCTTCGTG
    GAGTATGAGG TCCCCGAAGC TGCACAGCTG GCCTTGGAGC AGATGAACTC GGTGATGCTG
    GGGGGCAGGA ACATCAAGGT GGGCAGACCC AGCAACATAG GGCAGGCCCA GCCCATCATA
    GACCAGTTGG CTGAGGAGGC ACGGGCCTTC AACCGCATCT ACGTGGCCTC TGTGCACCAG
    GACCTCTCAG ACGATGACAT CAAGAGCGTG TTTGAGGCCT TTGGCAAGAT CAAGTCCTGC
    ACACTGGCCC GGGACCCCAC AACTGGCAAG CACAAGGGCT ACGGCTTCAT TGAGTACGAG
    AAGGCCCAGT CGTCCCAAGA TGCTGTGTCT TCCATGAACC TCTTTGACCT GGGTGGCCAG
    TACTTGCGGG TGGGCAAGGC TGTCACACCG CCCATGCCCC TACTCACACC AGCCACGCCT
    GGAGGCCTCC CACCTGCCGC TGCTGTGGCA GCTGCTGCAG CCACTGCCAA GATCACAGCT
    CAGGAAGCAG TGGCCGGAGC AGCGGTGCTG GGTACCCTGG GCACACCTGG ACTGGTGTCC
    CCAGCACTGA CCCTGGCCCA GCCCCTGGGC ACTTTGCCCC AGGCTGTCAT GGCTGCCCAG
    GCACCTGGAG TCATCACAGG TGTGACCCCA GCCCGTCCTC CTATCCCGGT CACCATCCCC
    TCGGTGGGAG TGGTGAACCC CATCCTGGCC AGCCCTCCAA CGCTGGGTCT CCTGGAGCCC
    AAGAAGGAGA AGGAAGAAGA GGAGCTGTTT CCCGAGTCAG AGCGGCCAGA GATGCTGAGC
    GAGCAGGAGC ACATGAGCAT CTCGGGCAGT AGCGCCCGAC ACATGGTGAT GCAGAAGCTG
    CTCCGCAAGC AGGAGTCTAC AGTGATGGTT CTGCGCAACA TGGTGGACCC CAAGGACATC
    GATGATGACC TGGAAGGGGA GGTGACAGAG GAGTGTGGCA AGTTCGGGGC CGTGAACCGC
    GTCATCATCT ACCAAGAGAA ACAAGGCGAG GAGGAGGATG CAGAAATCAT TGTCAAGATC
    TTTGTGGAGT TTTCCATAGC CTCTGAGACT CATAAGGCCA TCCAGGCCCT CAATGGCCGC
    TGGTTTGCTG GCCGCAAGGT GGTGGCTGAA GTGTACGACC AGGAGCGTTT TGATAACAGT
    GACCTCTCTG CGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016868724.1
    CDS111..1865
    Translation

    Target ORF information:

    RefSeq Version XM_017013235.1
    Organism Homo sapiens(human)
    Definition Homo sapiens poly(U) binding splicing factor 60 (PUF60), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017013235.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGCTGCTTA AAAGTGTCTT CAGGAGAGAA CTGGGACCTG GGCTGGGCCA GAGCCTGTGG 
    AAGGCACTGG AGCTGGGGCT GAGTTGTGGG CTGGCCAAGC AGGTCAATGG CCAGCAAGGA
    GGGGGGTCCG AGCCGGCGGC GGCGGCGGCA GTGGTGGCAG CGGGAGACAA ATGGAAACCT
    CCACAGGGCA CAGACTCCAT CAAGATGGAG AACGGGCAGA GCACAGCCGC CAAGCTGGGG
    CTGCCTCCCC TGACGCCCGA GCAGCAGGAG GCCCTTCAGA AGGCCAAGAA GTACGCCATG
    GAGCAGAGCA TCAAGAGTGT GCTGGTGAAG CAGACCATCG CGCACCAGCA GCAGCAGCTC
    ACCAACCTGC AGATGGCAGC AGTGACAATG GGCTTTGGAG ATCCTCTCTC ACCTTTGCAA
    TCGATGGCGG CTCAGCGGCA GCGGGCGCTG GCCATCATGT GCCGCGTCTA CGTGGGCTCT
    ATCTACTATG AGCTGGGGGA GGACACCATC CGCCAGGCCT TTGCCCCCTT TGGCCCCATC
    AAGAGCATCG ACATGTCCTG GGACTCCGTC ACCATGAAGC ACAAGGGCTT TGCCTTCGTG
    GAGTATGAGG TCCCCGAAGC TGCACAGCTG GCCTTGGAGC AGATGAACTC GGTGATGCTG
    GGGGGCAGGA ACATCAAGGT GGGCAGACCC AGCAACATAG GGCAGGCCCA GCCCATCATA
    GACCAGTTGG CTGAGGAGGC ACGGGCCTTC AACCGCATCT ACGTGGCCTC TGTGCACCAG
    GACCTCTCAG ACGATGACAT CAAGAGCGTG TTTGAGGCCT TTGGCAAGAT CAAGTCCTGC
    ACACTGGCCC GGGACCCCAC AACTGGCAAG CACAAGGGCT ACGGCTTCAT TGAGTACGAG
    AAGGCCCAGT CGTCCCAAGA TGCTGTGTCT TCCATGAACC TCTTTGACCT GGGTGGCCAG
    TACTTGCGGG TGGGCAAGGC TGTCACACCG CCCATGCCCC TACTCACACC AGCCACGCCT
    GGAGGCCTCC CACCTGCCGC TGCTGTGGCA GCTGCTGCAG CCACTGCCAA GATCACAGCT
    CAGGAAGCAG TGGCCGGAGC AGCGGTGCTG GGTACCCTGG GCACACCTGG ACTGGTGTCC
    CCAGCACTGA CCCTGGCCCA GCCCCTGGGC ACTTTGCCCC AGGCTGTCAT GGCTGCCCAG
    GCACCTGGAG TCATCACAGG TGTGACCCCA GCCCGTCCTC CTATCCCGGT CACCATCCCC
    TCGGTGGGAG TGGTGAACCC CATCCTGGCC AGCCCTCCAA CGCTGGGTCT CCTGGAGCCC
    AAGAAGGAGA AGGAAGAAGA GGAGCTGTTT CCCGAGTCAG AGCGGCCAGA GATGCTGAGC
    GAGCAGGAGC ACATGAGCAT CTCGGGCAGT AGCGCCCGAC ACATGGTGAT GCAGAAGCTG
    CTCCGCAAGC AGGAGTCTAC AGTGATGGTT CTGCGCAACA TGGTGGACCC CAAGGACATC
    GATGATGACC TGGAAGGGGA GGTGACAGAG GAGTGTGGCA AGTTCGGGGC CGTGAACCGC
    GTCATCATCT ACCAAGAGAA ACAAGGCGAG GAGGAGGATG CAGAAATCAT TGTCAAGATC
    TTTGTGGAGT TTTCCATAGC CTCTGAGACT CATAAGGCCA TCCAGGCCCT CAATGGCCGC
    TGGTTTGCTG GCCGCAAGGT GGTGGCTGAA GTGTACGACC AGGAGCGTTT TGATAACAGT
    GACCTCTCTG CGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.