PUF60 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	PUF60
Entrez Gene ID	22827
Full Name	poly(U) binding splicing factor 60
Synonyms	FIR,RoBPI,SIAHBP1,VRJS
General protein information	Preferred Names poly(U) binding splicing factor 60 Names poly(U)-binding-splicing factor PUF60 FBP interacting repressor FUSE-binding protein-interacting repressor Ro ribonucleoprotein-binding protein 1 Siah binding protein 1 poly(U) binding splicing factor 60KDa pyrimidine tract binding splicing factor
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 8 Map Location: 8q24.3
Summary	This gene encodes a nucleic acid-binding protein that plays a role in a variety of nuclear processes, including pre-mRNA splicing and transcriptional regulation. The encoded protein forms a complex with the far upstream DNA element (FUSE) and FUSE-binding protein at the myelocytomatosis oncogene (MYC) promoter. This complex represses MYC transcription through the core-TFIIH basal transcription factor. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Aug 2012].
Disorder MIM:	604819
Disorder Html:	Verheij syndrome, 615583 (3)

mRNA and Protein(s)

mRNA	Protein	Name
XM_011516929.2	XP_011515231.1	poly(U)-binding-splicing factor PUF60 isoform X1
NM_001136033.2	NP_001129505.1	poly(U)-binding-splicing factor PUF60 isoform c
NM_001362897.1	NP_001349826.1	poly(U)-binding-splicing factor PUF60 isoform j
NM_001271097.1	NP_001258026.1	poly(U)-binding-splicing factor PUF60 isoform e
NM_001362896.2	NP_001349825.1	poly(U)-binding-splicing factor PUF60 isoform i
NM_001271099.2	NP_001258028.1	poly(U)-binding-splicing factor PUF60 isoform g
NM_001271096.2	NP_001258025.1	poly(U)-binding-splicing factor PUF60 isoform d
XM_017013236.1	XP_016868725.1	poly(U)-binding-splicing factor PUF60 isoform X9
NM_001362897.2	NP_001349826.1	poly(U)-binding-splicing factor PUF60 isoform j
NM_001271100.1	NP_001258029.1	poly(U)-binding-splicing factor PUF60 isoform h
NM_014281.4	NP_055096.2	poly(U)-binding-splicing factor PUF60 isoform b
NM_001271097.2	NP_001258026.1	poly(U)-binding-splicing factor PUF60 isoform e
NM_001271100.2	NP_001258029.1	poly(U)-binding-splicing factor PUF60 isoform h
NM_001136033.3	NP_001129505.1	poly(U)-binding-splicing factor PUF60 isoform c
NM_014281.5	NP_055096.2	poly(U)-binding-splicing factor PUF60 isoform b
NM_001271098.2	NP_001258027.1	poly(U)-binding-splicing factor PUF60 isoform f
XM_017013239.1	XP_016868728.1	poly(U)-binding-splicing factor PUF60 isoform X13
NM_001362895.1	NP_001349824.1	poly(U)-binding-splicing factor PUF60 isoform i
NM_001362896.1	NP_001349825.1	poly(U)-binding-splicing factor PUF60 isoform i
XM_017013238.1	XP_016868727.1	poly(U)-binding-splicing factor PUF60 isoform X1
XM_017013237.1	XP_016868726.1	poly(U)-binding-splicing factor PUF60 isoform X1
XM_017013234.1	XP_016868723.1	poly(U)-binding-splicing factor PUF60 isoform X5
XM_017013235.1	XP_016868724.1	poly(U)-binding-splicing factor PUF60 isoform X6
XM_017013240.1	XP_016868729.1	poly(U)-binding-splicing factor PUF60 isoform X14
XM_011516930.2	XP_011515232.1	poly(U)-binding-splicing factor PUF60 isoform X3
NM_078480.2	NP_510965.1	poly(U)-binding-splicing factor PUF60 isoform a
NM_001271099.1	NP_001258028.1	poly(U)-binding-splicing factor PUF60 isoform g
NM_001271096.1	NP_001258025.1	poly(U)-binding-splicing factor PUF60 isoform d
NM_001271098.1	NP_001258027.1	poly(U)-binding-splicing factor PUF60 isoform f
NM_078480.3	NP_510965.1	poly(U)-binding-splicing factor PUF60 isoform a
NM_001362895.2	NP_001349824.1	poly(U)-binding-splicing factor PUF60 isoform i

Pathways from BioSystems

Homologies

Bos taurus (cattle)	PUF60	NP_001039598.1
	PUF60	XP_001087975.2
Mus musculus (house mouse)	Puf60	NP_082640.2
Rattus norvegicus (Norway rat)	Puf60	NP_001178809.1
Drosophila melanogaster (fruit fly)	pUf68	NP_525123.2
Caenorhabditis elegans (roundworm)	rnp-6	NP_491176.1
Canis lupus familiaris (dog)	PUF60	XP_005628101.1
Danio rerio (zebrafish)	puf60a	NP_001099058.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010553	XP_314526.3
Homo sapiens (human)	PUF60	NP_510965.1
Pan troglodytes (chimpanzee)	PUF60	XP_528257.3
Gallus gallus (chicken)	PUF60	NP_001026551.1
Xenopus tropicalis (tropical clawed frog)	puf60	NP_001120082.1

Gene Ontology
Bibliography

Related articles in PubMed

Exome sequencing reveals NAA15 and PUF60 as candidate genes associated with intellectual disability.
Zhao JJ, Halvardson J, Zander CS, Zaghlool A, Georgii-Hemming P, M?nsson E, Brandberg G, S?vmarker HE, Frykholm C, Kuchinskaya E, Thuresson AC, Feuk L
American journal of medical genetics. Part B, Neuropsychiatric genetics : the official publication of the International Society of Psychiatric Genetics177(1)10-20(2018 Jan)

A de novo PUF60 mutation in a child with a syndromic form of coloboma and persistent fetal vasculature.
Graziano C, Gusson E, Severi G, Isidori F, Wischmeijer A, Brugnara M, Seri M, Rossi C
Ophthalmic genetics38(6)590-592(2017 Dec)

Involvement of PUF60 in Transcriptional and Post-transcriptional Regulation of Hepatitis B Virus Pregenomic RNA Expression.
Sun S, Nakashima K, Ito M, Li Y, Chida T, Takahashi H, Watashi K, Sawasaki T, Wakita T, Suzuki T
Scientific reports7(1)12874(2017 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Heterozygote loss-of-function variants in PUF60 cause a phenotype comprising growth/developmental delay and craniofacial, cardiac, renal, ocular and spinal anomalies.
Title: PUF60 variants cause a syndrome of ID, short stature, microcephaly, coloboma, craniofacial, cardiac, renal and spinal features.

These results confirm that PUF60 is a major driver for the developmental, craniofacial, skeletal and cardiac phenotypes associated with the 8q24.3 microdeletion
Title: Dominant variants in the splicing factor PUF60 cause a recognizable syndrome with intellectual disability, heart defects and short stature.

Mutations in PUF60 gene is associated with idiopathic hypereosinophilic syndrome.
Title: Whole-exome sequencing and genome-wide methylation analyses identify novel disease associated mutations and methylation patterns in idiopathic hypereosinophilic syndrome.

Concomitant over expression of far upstream element (FUSE) binding protein (FBP) interacting repressor (FIR) and its splice variants induce migration and invasion of non-small cell lung cancer cells.
Title: Concomitant expression of far upstream element (FUSE) binding protein (FBP) interacting repressor (FIR) and its splice variants induce migration and invasion of non-small cell lung cancer (NSCLC) cells.

Overexpression of far upstream element (FUSE) binding protein (FBP)-interacting repressor (FIR) supports growth of hepatocellular carcinoma.
Title: Overexpression of far upstream element (FUSE) binding protein (FBP)-interacting repressor (FIR) supports growth of hepatocellular carcinoma.

The following PUF60 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PUF60 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu20077	NM_014281.5 Latest version!	Homo sapiens poly(U) binding splicing factor 60 (PUF60), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$219.50-$307.30 $439.00
OHu22582	NM_001136033.3 Latest version!	Homo sapiens poly(U) binding splicing factor 60 (PUF60), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$219.50-$307.30 $439.00
OHu23020	NM_001271100.2 Latest version!	Homo sapiens poly(U) binding splicing factor 60 (PUF60), transcript variant 8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
OHu22582	XM_017013239.1 Latest version!	Homo sapiens poly(U) binding splicing factor 60 (PUF60), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$219.50-$307.30 $439.00
OHu23020	XM_017013240.1 Latest version!	Homo sapiens poly(U) binding splicing factor 60 (PUF60), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
OHu20314	NM_078480.3 Latest version!	Homo sapiens poly(U) binding splicing factor 60 (PUF60), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu20744	NM_001271096.2 Latest version!	Homo sapiens poly(U) binding splicing factor 60 (PUF60), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu21541	NM_001271098.2 Latest version!	Homo sapiens poly(U) binding splicing factor 60 (PUF60), transcript variant 6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu27468	NM_001271099.2 Latest version!	Homo sapiens poly(U) binding splicing factor 60 (PUF60), transcript variant 7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu27624	NM_001271097.2 Latest version!	Homo sapiens poly(U) binding splicing factor 60 (PUF60), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu64963	NM_001362895.2 Latest version!	Homo sapiens poly(U) binding splicing factor 60 (PUF60), transcript variant 9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu64963	NM_001362896.2 Latest version!	Homo sapiens poly(U) binding splicing factor 60 (PUF60), transcript variant 10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu64964	NM_001362897.2 Latest version!	Homo sapiens poly(U) binding splicing factor 60 (PUF60), transcript variant 11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu64963	XM_017013234.1 Latest version!	Homo sapiens poly(U) binding splicing factor 60 (PUF60), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu64963	XM_017013238.1 Latest version!	Homo sapiens poly(U) binding splicing factor 60KDa (PUF60), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu64963	XM_017013237.1 Latest version!	Homo sapiens poly(U) binding splicing factor 60KDa (PUF60), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu64963	XM_011516929.2 Latest version!	Homo sapiens poly(U) binding splicing factor 60KDa (PUF60), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu64964	XM_017013236.1 Latest version!	Homo sapiens poly(U) binding splicing factor 60 (PUF60), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu64964	XM_011516930.2 Latest version!	Homo sapiens poly(U) binding splicing factor 60KDa (PUF60), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu97848	XM_017013235.1 Latest version!	Homo sapiens poly(U) binding splicing factor 60 (PUF60), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
View Old Accession Versions >>
OHu64964	NM_001362897.1	Homo sapiens poly(U) binding splicing factor 60 (PUF60), transcript variant 11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu27624	NM_001271097.1	Homo sapiens poly(U) binding splicing factor 60 (PUF60), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu23020	NM_001271100.1	Homo sapiens poly(U) binding splicing factor 60 (PUF60), transcript variant 8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
OHu22582	NM_001136033.2	Homo sapiens poly(U) binding splicing factor 60 (PUF60), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$219.50-$307.30 $439.00
OHu20077	NM_014281.4	Homo sapiens poly(U) binding splicing factor 60 (PUF60), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$219.50-$307.30 $439.00
OHu64963	NM_001362896.1	Homo sapiens poly(U) binding splicing factor 60 (PUF60), transcript variant 10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu27468	NM_001271099.1	Homo sapiens poly(U) binding splicing factor 60 (PUF60), transcript variant 7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu20744	NM_001271096.1	Homo sapiens poly(U) binding splicing factor 60 (PUF60), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu21541	NM_001271098.1	Homo sapiens poly(U) binding splicing factor 60 (PUF60), transcript variant 6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu20314	NM_078480.2	Homo sapiens poly(U) binding splicing factor 60 (PUF60), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu64963	NM_001362895.1	Homo sapiens poly(U) binding splicing factor 60 (PUF60), transcript variant 9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PUF60 ( NM_014281.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_055096.2 Homo sapiens poly(U) binding splicing factor 60 (PUF60), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu20077
Clone ID Related Accession (Same CDS sequence)	NM_014281.4 , NM_014281.5
Accession Version	NM_014281.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1629bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-10-20
Organism	Homo sapiens(human)
Product	poly(U)-binding-splicing factor PUF60 isoform b
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CN371576.1, AF217197.2 and AW249619.1. On Sep 1, 2012 this sequence version replaced NM_014281.3. Transcript Variant: This variant (2) lacks an alternate in-frame exon in the coding region, compared to variant 1. The encoded isoform (b) is shorter than isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163655.487795.1, BC008875.2 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGACGG CGACCATAGC TCTCCAGGTC AATGGCCAGC AAGGAGGGGG GTCCGAGCCG 
GCGGCGGCGG CGGCAGTGGT GGCAGCGGGA GACAAATGGA AACCTCCACA GGGCACAGAC 
TCCATCAAGA TGGAGAACGG GCAGAGCACA GCCGCCAAGC TGGGGCTGCC TCCCCTGACG 
CCCGAGCAGC AGGAGGCCCT TCAGAAGGCC AAGAAGTACG CCATGGAGCA GAGCATCAAG 
AGTGTGCTGG TGAAGCAGAC CATCGCGCAC CAGCAGCAGC AGCTCACCAA CCTGCAGATG 
GCGGCTCAGC GGCAGCGGGC GCTGGCCATC ATGTGCCGCG TCTACGTGGG CTCTATCTAC 
TATGAGCTGG GGGAGGACAC CATCCGCCAG GCCTTTGCCC CCTTTGGCCC CATCAAGAGC 
ATCGACATGT CCTGGGACTC CGTCACCATG AAGCACAAGG GCTTTGCCTT CGTGGAGTAT 
GAGGTCCCCG AAGCTGCACA GCTGGCCTTG GAGCAGATGA ACTCGGTGAT GCTGGGGGGC 
AGGAACATCA AGGTGGGCAG ACCCAGCAAC ATAGGGCAGG CCCAGCCCAT CATAGACCAG 
TTGGCTGAGG AGGCACGGGC CTTCAACCGC ATCTACGTGG CCTCTGTGCA CCAGGACCTC 
TCAGACGATG ACATCAAGAG CGTGTTTGAG GCCTTTGGCA AGATCAAGTC CTGCACACTG 
GCCCGGGACC CCACAACTGG CAAGCACAAG GGCTACGGCT TCATTGAGTA CGAGAAGGCC 
CAGTCGTCCC AAGATGCTGT GTCTTCCATG AACCTCTTTG ACCTGGGTGG CCAGTACTTG 
CGGGTGGGCA AGGCTGTCAC ACCGCCCATG CCCCTACTCA CACCAGCCAC GCCTGGAGGC 
CTCCCACCTG CCGCTGCTGT GGCAGCTGCT GCAGCCACTG CCAAGATCAC AGCTCAGGAA 
GCAGTGGCCG GAGCAGCGGT GCTGGGTACC CTGGGCACAC CTGGACTGGT GTCCCCAGCA 
CTGACCCTGG CCCAGCCCCT GGGCACTTTG CCCCAGGCTG TCATGGCTGC CCAGGCACCT 
GGAGTCATCA CAGGTGTGAC CCCAGCCCGT CCTCCTATCC CGGTCACCAT CCCCTCGGTG 
GGAGTGGTGA ACCCCATCCT GGCCAGCCCT CCAACGCTGG GTCTCCTGGA GCCCAAGAAG 
GAGAAGGAAG AAGAGGAGCT GTTTCCCGAG TCAGAGCGGC CAGAGATGCT GAGCGAGCAG 
GAGCACATGA GCATCTCGGG CAGTAGCGCC CGACACATGG TGATGCAGAA GCTGCTCCGC 
AAGCAGGAGT CTACAGTGAT GGTTCTGCGC AACATGGTGG ACCCCAAGGA CATCGATGAT 
GACCTGGAAG GGGAGGTGAC AGAGGAGTGT GGCAAGTTCG GGGCCGTGAA CCGCGTCATC 
ATCTACCAAG AGAAACAAGG CGAGGAGGAG GATGCAGAAA TCATTGTCAA GATCTTTGTG 
GAGTTTTCCA TAGCCTCTGA GACTCATAAG GCCATCCAGG CCCTCAATGG CCGCTGGTTT 
GCTGGCCGCA AGGTGGTGGC TGAAGTGTAC GACCAGGAGC GTTTTGATAA CAGTGACCTC 
TCTGCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_055096.2
CDS	84..1712
Translation	MATATIALQVNGQQGGGSEPAAAAAVVAAGDKWKPPQGTDSIKMENGQSTAAKLGLPPLTPEQQEALQKAKKYAMEQSIKSVLVKQTIAHQQQQLTNLQMAAQRQRALAIMCRVYVGSIYYELGEDTIRQAFAPFGPIKSIDMSWDSVTMKHKGFAFVEYEVPEAAQLALEQMNSVMLGGRNIKVGRPSNIGQAQPIIDQLAEEARAFNRIYVASVHQDLSDDDIKSVFEAFGKIKSCTLARDPTTGKHKGYGFIEYEKAQSSQDAVSSMNLFDLGGQYLRVGKAVTPPMPLLTPATPGGLPPAAAVAAAAATAKITAQEAVAGAAVLGTLGTPGLVSPALTLAQPLGTLPQAVMAAQAPGVITGVTPARPPIPVTIPSVGVVNPILASPPTLGLLEPKKEKEEEELFPESERPEMLSEQEHMSISGSSARHMVMQKLLRKQESTVMVLRNMVDPKDIDDDLEGEVTEECGKFGAVNRVIIYQEKQGEEEDAEIIVKIFVEFSIASETHKAIQALNGRWFAGRKVVAEVYDQERFDNSDLSA

Target ORF information:

RefSeq Version	NM_014281.4
Organism	Homo sapiens(human)
Definition	Homo sapiens poly(U) binding splicing factor 60 (PUF60), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_014281.4

ORF Insert Sequence:

ATGGCGACGG CGACCATAGC TCTCCAGGTC AATGGCCAGC AAGGAGGGGG GTCCGAGCCG 
GCGGCGGCGG CGGCAGTGGT GGCAGCGGGA GACAAATGGA AACCTCCACA GGGCACAGAC 
TCCATCAAGA TGGAGAACGG GCAGAGCACA GCCGCCAAGC TGGGGCTGCC TCCCCTGACG 
CCCGAGCAGC AGGAGGCCCT TCAGAAGGCC AAGAAGTACG CCATGGAGCA GAGCATCAAG 
AGTGTGCTGG TGAAGCAGAC CATCGCGCAC CAGCAGCAGC AGCTCACCAA CCTGCAGATG 
GCGGCTCAGC GGCAGCGGGC GCTGGCCATC ATGTGCCGCG TCTACGTGGG CTCTATCTAC 
TATGAGCTGG GGGAGGACAC CATCCGCCAG GCCTTTGCCC CCTTTGGCCC CATCAAGAGC 
ATCGACATGT CCTGGGACTC CGTCACCATG AAGCACAAGG GCTTTGCCTT CGTGGAGTAT 
GAGGTCCCCG AAGCTGCACA GCTGGCCTTG GAGCAGATGA ACTCGGTGAT GCTGGGGGGC 
AGGAACATCA AGGTGGGCAG ACCCAGCAAC ATAGGGCAGG CCCAGCCCAT CATAGACCAG 
TTGGCTGAGG AGGCACGGGC CTTCAACCGC ATCTACGTGG CCTCTGTGCA CCAGGACCTC 
TCAGACGATG ACATCAAGAG CGTGTTTGAG GCCTTTGGCA AGATCAAGTC CTGCACACTG 
GCCCGGGACC CCACAACTGG CAAGCACAAG GGCTACGGCT TCATTGAGTA CGAGAAGGCC 
CAGTCGTCCC AAGATGCTGT GTCTTCCATG AACCTCTTTG ACCTGGGTGG CCAGTACTTG 
CGGGTGGGCA AGGCTGTCAC ACCGCCCATG CCCCTACTCA CACCAGCCAC GCCTGGAGGC 
CTCCCACCTG CCGCTGCTGT GGCAGCTGCT GCAGCCACTG CCAAGATCAC AGCTCAGGAA 
GCAGTGGCCG GAGCAGCGGT GCTGGGTACC CTGGGCACAC CTGGACTGGT GTCCCCAGCA 
CTGACCCTGG CCCAGCCCCT GGGCACTTTG CCCCAGGCTG TCATGGCTGC CCAGGCACCT 
GGAGTCATCA CAGGTGTGAC CCCAGCCCGT CCTCCTATCC CGGTCACCAT CCCCTCGGTG 
GGAGTGGTGA ACCCCATCCT GGCCAGCCCT CCAACGCTGG GTCTCCTGGA GCCCAAGAAG 
GAGAAGGAAG AAGAGGAGCT GTTTCCCGAG TCAGAGCGGC CAGAGATGCT GAGCGAGCAG 
GAGCACATGA GCATCTCGGG CAGTAGCGCC CGACACATGG TGATGCAGAA GCTGCTCCGC 
AAGCAGGAGT CTACAGTGAT GGTTCTGCGC AACATGGTGG ACCCCAAGGA CATCGATGAT 
GACCTGGAAG GGGAGGTGAC AGAGGAGTGT GGCAAGTTCG GGGCCGTGAA CCGCGTCATC 
ATCTACCAAG AGAAACAAGG CGAGGAGGAG GATGCAGAAA TCATTGTCAA GATCTTTGTG 
GAGTTTTCCA TAGCCTCTGA GACTCATAAG GCCATCCAGG CCCTCAATGG CCGCTGGTTT 
GCTGGCCGCA AGGTGGTGGC TGAAGTGTAC GACCAGGAGC GTTTTGATAA CAGTGACCTC 
TCTGCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PUF60 ( NM_014281.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_055096.2 Homo sapiens poly(U) binding splicing factor 60 (PUF60), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu20077
Clone ID Related Accession (Same CDS sequence)	NM_014281.4 , NM_014281.5
Accession Version	NM_014281.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1629bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-27
Organism	Homo sapiens(human)
Product	poly(U)-binding-splicing factor PUF60 isoform b
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CN371576.1, AF217197.2 and AW249619.1. On May 31, 2019 this sequence version replaced NM_014281.4. Transcript Variant: This variant (2) lacks an alternate in-frame exon in the coding region, compared to variant 1. The encoded isoform (b) is shorter than isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.86649.1, SRR1803615.37601.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGACGG CGACCATAGC TCTCCAGGTC AATGGCCAGC AAGGAGGGGG GTCCGAGCCG 
GCGGCGGCGG CGGCAGTGGT GGCAGCGGGA GACAAATGGA AACCTCCACA GGGCACAGAC 
TCCATCAAGA TGGAGAACGG GCAGAGCACA GCCGCCAAGC TGGGGCTGCC TCCCCTGACG 
CCCGAGCAGC AGGAGGCCCT TCAGAAGGCC AAGAAGTACG CCATGGAGCA GAGCATCAAG 
AGTGTGCTGG TGAAGCAGAC CATCGCGCAC CAGCAGCAGC AGCTCACCAA CCTGCAGATG 
GCGGCTCAGC GGCAGCGGGC GCTGGCCATC ATGTGCCGCG TCTACGTGGG CTCTATCTAC 
TATGAGCTGG GGGAGGACAC CATCCGCCAG GCCTTTGCCC CCTTTGGCCC CATCAAGAGC 
ATCGACATGT CCTGGGACTC CGTCACCATG AAGCACAAGG GCTTTGCCTT CGTGGAGTAT 
GAGGTCCCCG AAGCTGCACA GCTGGCCTTG GAGCAGATGA ACTCGGTGAT GCTGGGGGGC 
AGGAACATCA AGGTGGGCAG ACCCAGCAAC ATAGGGCAGG CCCAGCCCAT CATAGACCAG 
TTGGCTGAGG AGGCACGGGC CTTCAACCGC ATCTACGTGG CCTCTGTGCA CCAGGACCTC 
TCAGACGATG ACATCAAGAG CGTGTTTGAG GCCTTTGGCA AGATCAAGTC CTGCACACTG 
GCCCGGGACC CCACAACTGG CAAGCACAAG GGCTACGGCT TCATTGAGTA CGAGAAGGCC 
CAGTCGTCCC AAGATGCTGT GTCTTCCATG AACCTCTTTG ACCTGGGTGG CCAGTACTTG 
CGGGTGGGCA AGGCTGTCAC ACCGCCCATG CCCCTACTCA CACCAGCCAC GCCTGGAGGC 
CTCCCACCTG CCGCTGCTGT GGCAGCTGCT GCAGCCACTG CCAAGATCAC AGCTCAGGAA 
GCAGTGGCCG GAGCAGCGGT GCTGGGTACC CTGGGCACAC CTGGACTGGT GTCCCCAGCA 
CTGACCCTGG CCCAGCCCCT GGGCACTTTG CCCCAGGCTG TCATGGCTGC CCAGGCACCT 
GGAGTCATCA CAGGTGTGAC CCCAGCCCGT CCTCCTATCC CGGTCACCAT CCCCTCGGTG 
GGAGTGGTGA ACCCCATCCT GGCCAGCCCT CCAACGCTGG GTCTCCTGGA GCCCAAGAAG 
GAGAAGGAAG AAGAGGAGCT GTTTCCCGAG TCAGAGCGGC CAGAGATGCT GAGCGAGCAG 
GAGCACATGA GCATCTCGGG CAGTAGCGCC CGACACATGG TGATGCAGAA GCTGCTCCGC 
AAGCAGGAGT CTACAGTGAT GGTTCTGCGC AACATGGTGG ACCCCAAGGA CATCGATGAT 
GACCTGGAAG GGGAGGTGAC AGAGGAGTGT GGCAAGTTCG GGGCCGTGAA CCGCGTCATC 
ATCTACCAAG AGAAACAAGG CGAGGAGGAG GATGCAGAAA TCATTGTCAA GATCTTTGTG 
GAGTTTTCCA TAGCCTCTGA GACTCATAAG GCCATCCAGG CCCTCAATGG CCGCTGGTTT 
GCTGGCCGCA AGGTGGTGGC TGAAGTGTAC GACCAGGAGC GTTTTGATAA CAGTGACCTC 
TCTGCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_055096.2
CDS	13..1641
Translation	MATATIALQVNGQQGGGSEPAAAAAVVAAGDKWKPPQGTDSIKMENGQSTAAKLGLPPLTPEQQEALQKAKKYAMEQSIKSVLVKQTIAHQQQQLTNLQMAAQRQRALAIMCRVYVGSIYYELGEDTIRQAFAPFGPIKSIDMSWDSVTMKHKGFAFVEYEVPEAAQLALEQMNSVMLGGRNIKVGRPSNIGQAQPIIDQLAEEARAFNRIYVASVHQDLSDDDIKSVFEAFGKIKSCTLARDPTTGKHKGYGFIEYEKAQSSQDAVSSMNLFDLGGQYLRVGKAVTPPMPLLTPATPGGLPPAAAVAAAAATAKITAQEAVAGAAVLGTLGTPGLVSPALTLAQPLGTLPQAVMAAQAPGVITGVTPARPPIPVTIPSVGVVNPILASPPTLGLLEPKKEKEEEELFPESERPEMLSEQEHMSISGSSARHMVMQKLLRKQESTVMVLRNMVDPKDIDDDLEGEVTEECGKFGAVNRVIIYQEKQGEEEDAEIIVKIFVEFSIASETHKAIQALNGRWFAGRKVVAEVYDQERFDNSDLSA

Target ORF information:

RefSeq Version	NM_014281.5
Organism	Homo sapiens(human)
Definition	Homo sapiens poly(U) binding splicing factor 60 (PUF60), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_014281.5

ORF Insert Sequence:

ATGGCGACGG CGACCATAGC TCTCCAGGTC AATGGCCAGC AAGGAGGGGG GTCCGAGCCG 
GCGGCGGCGG CGGCAGTGGT GGCAGCGGGA GACAAATGGA AACCTCCACA GGGCACAGAC 
TCCATCAAGA TGGAGAACGG GCAGAGCACA GCCGCCAAGC TGGGGCTGCC TCCCCTGACG 
CCCGAGCAGC AGGAGGCCCT TCAGAAGGCC AAGAAGTACG CCATGGAGCA GAGCATCAAG 
AGTGTGCTGG TGAAGCAGAC CATCGCGCAC CAGCAGCAGC AGCTCACCAA CCTGCAGATG 
GCGGCTCAGC GGCAGCGGGC GCTGGCCATC ATGTGCCGCG TCTACGTGGG CTCTATCTAC 
TATGAGCTGG GGGAGGACAC CATCCGCCAG GCCTTTGCCC CCTTTGGCCC CATCAAGAGC 
ATCGACATGT CCTGGGACTC CGTCACCATG AAGCACAAGG GCTTTGCCTT CGTGGAGTAT 
GAGGTCCCCG AAGCTGCACA GCTGGCCTTG GAGCAGATGA ACTCGGTGAT GCTGGGGGGC 
AGGAACATCA AGGTGGGCAG ACCCAGCAAC ATAGGGCAGG CCCAGCCCAT CATAGACCAG 
TTGGCTGAGG AGGCACGGGC CTTCAACCGC ATCTACGTGG CCTCTGTGCA CCAGGACCTC 
TCAGACGATG ACATCAAGAG CGTGTTTGAG GCCTTTGGCA AGATCAAGTC CTGCACACTG 
GCCCGGGACC CCACAACTGG CAAGCACAAG GGCTACGGCT TCATTGAGTA CGAGAAGGCC 
CAGTCGTCCC AAGATGCTGT GTCTTCCATG AACCTCTTTG ACCTGGGTGG CCAGTACTTG 
CGGGTGGGCA AGGCTGTCAC ACCGCCCATG CCCCTACTCA CACCAGCCAC GCCTGGAGGC 
CTCCCACCTG CCGCTGCTGT GGCAGCTGCT GCAGCCACTG CCAAGATCAC AGCTCAGGAA 
GCAGTGGCCG GAGCAGCGGT GCTGGGTACC CTGGGCACAC CTGGACTGGT GTCCCCAGCA 
CTGACCCTGG CCCAGCCCCT GGGCACTTTG CCCCAGGCTG TCATGGCTGC CCAGGCACCT 
GGAGTCATCA CAGGTGTGAC CCCAGCCCGT CCTCCTATCC CGGTCACCAT CCCCTCGGTG 
GGAGTGGTGA ACCCCATCCT GGCCAGCCCT CCAACGCTGG GTCTCCTGGA GCCCAAGAAG 
GAGAAGGAAG AAGAGGAGCT GTTTCCCGAG TCAGAGCGGC CAGAGATGCT GAGCGAGCAG 
GAGCACATGA GCATCTCGGG CAGTAGCGCC CGACACATGG TGATGCAGAA GCTGCTCCGC 
AAGCAGGAGT CTACAGTGAT GGTTCTGCGC AACATGGTGG ACCCCAAGGA CATCGATGAT 
GACCTGGAAG GGGAGGTGAC AGAGGAGTGT GGCAAGTTCG GGGCCGTGAA CCGCGTCATC 
ATCTACCAAG AGAAACAAGG CGAGGAGGAG GATGCAGAAA TCATTGTCAA GATCTTTGTG 
GAGTTTTCCA TAGCCTCTGA GACTCATAAG GCCATCCAGG CCCTCAATGG CCGCTGGTTT 
GCTGGCCGCA AGGTGGTGGC TGAAGTGTAC GACCAGGAGC GTTTTGATAA CAGTGACCTC 
TCTGCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PUF60 ( NM_001136033.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001129505.1 Homo sapiens poly(U) binding splicing factor 60 (PUF60), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu22582
Clone ID Related Accession (Same CDS sequence)	NM_001136033.2 , NM_001136033.3 , XM_017013239.1
Accession Version	NM_001136033.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1551bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-10-20
Organism	Homo sapiens(human)
Product	poly(U)-binding-splicing factor PUF60 isoform c
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC234917.3, BG771821.1, BC011265.1 and AW249619.1. On Sep 1, 2012 this sequence version replaced NM_001136033.1. Transcript Variant: This variant (3) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at an alternate in-frame downstream start site, compared to variant 1. The encoded isoform (c) has a shorter N-terminus, compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC011265.1, SRR1660805.18195.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGAACG GGCAGAGCAC AGCCGCCAAG CTGGGGCTGC CTCCCCTGAC GCCCGAGCAG 
CAGGAGGCCC TTCAGAAGGC CAAGAAGTAC GCCATGGAGC AGAGCATCAA GAGTGTGCTG 
GTGAAGCAGA CCATCGCGCA CCAGCAGCAG CAGCTCACCA ACCTGCAGAT GGCAGCAGTG 
ACAATGGGCT TTGGAGATCC TCTCTCACCT TTGCAATCGA TGGCGGCTCA GCGGCAGCGG 
GCGCTGGCCA TCATGTGCCG CGTCTACGTG GGCTCTATCT ACTATGAGCT GGGGGAGGAC 
ACCATCCGCC AGGCCTTTGC CCCCTTTGGC CCCATCAAGA GCATCGACAT GTCCTGGGAC 
TCCGTCACCA TGAAGCACAA GGGCTTTGCC TTCGTGGAGT ATGAGGTCCC CGAAGCTGCA 
CAGCTGGCCT TGGAGCAGAT GAACTCGGTG ATGCTGGGGG GCAGGAACAT CAAGGTGGGC 
AGACCCAGCA ACATAGGGCA GGCCCAGCCC ATCATAGACC AGTTGGCTGA GGAGGCACGG 
GCCTTCAACC GCATCTACGT GGCCTCTGTG CACCAGGACC TCTCAGACGA TGACATCAAG 
AGCGTGTTTG AGGCCTTTGG CAAGATCAAG TCCTGCACAC TGGCCCGGGA CCCCACAACT 
GGCAAGCACA AGGGCTACGG CTTCATTGAG TACGAGAAGG CCCAGTCGTC CCAAGATGCT 
GTGTCTTCCA TGAACCTCTT TGACCTGGGT GGCCAGTACT TGCGGGTGGG CAAGGCTGTC 
ACACCGCCCA TGCCCCTACT CACACCAGCC ACGCCTGGAG GCCTCCCACC TGCCGCTGCT 
GTGGCAGCTG CTGCAGCCAC TGCCAAGATC ACAGCTCAGG AAGCAGTGGC CGGAGCAGCG 
GTGCTGGGTA CCCTGGGCAC ACCTGGACTG GTGTCCCCAG CACTGACCCT GGCCCAGCCC 
CTGGGCACTT TGCCCCAGGC TGTCATGGCT GCCCAGGCAC CTGGAGTCAT CACAGGTGTG 
ACCCCAGCCC GTCCTCCTAT CCCGGTCACC ATCCCCTCGG TGGGAGTGGT GAACCCCATC 
CTGGCCAGCC CTCCAACGCT GGGTCTCCTG GAGCCCAAGA AGGAGAAGGA AGAAGAGGAG 
CTGTTTCCCG AGTCAGAGCG GCCAGAGATG CTGAGCGAGC AGGAGCACAT GAGCATCTCG 
GGCAGTAGCG CCCGACACAT GGTGATGCAG AAGCTGCTCC GCAAGCAGGA GTCTACAGTG 
ATGGTTCTGC GCAACATGGT GGACCCCAAG GACATCGATG ATGACCTGGA AGGGGAGGTG 
ACAGAGGAGT GTGGCAAGTT CGGGGCCGTG AACCGCGTCA TCATCTACCA AGAGAAACAA 
GGCGAGGAGG AGGATGCAGA AATCATTGTC AAGATCTTTG TGGAGTTTTC CATAGCCTCT 
GAGACTCATA AGGCCATCCA GGCCCTCAAT GGCCGCTGGT TTGCTGGCCG CAAGGTGGTG 
GCTGAAGTGT ACGACCAGGA GCGTTTTGAT AACAGTGACC TCTCTGCGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001129505.1
CDS	232..1782
Translation	MENGQSTAAKLGLPPLTPEQQEALQKAKKYAMEQSIKSVLVKQTIAHQQQQLTNLQMAAVTMGFGDPLSPLQSMAAQRQRALAIMCRVYVGSIYYELGEDTIRQAFAPFGPIKSIDMSWDSVTMKHKGFAFVEYEVPEAAQLALEQMNSVMLGGRNIKVGRPSNIGQAQPIIDQLAEEARAFNRIYVASVHQDLSDDDIKSVFEAFGKIKSCTLARDPTTGKHKGYGFIEYEKAQSSQDAVSSMNLFDLGGQYLRVGKAVTPPMPLLTPATPGGLPPAAAVAAAAATAKITAQEAVAGAAVLGTLGTPGLVSPALTLAQPLGTLPQAVMAAQAPGVITGVTPARPPIPVTIPSVGVVNPILASPPTLGLLEPKKEKEEEELFPESERPEMLSEQEHMSISGSSARHMVMQKLLRKQESTVMVLRNMVDPKDIDDDLEGEVTEECGKFGAVNRVIIYQEKQGEEEDAEIIVKIFVEFSIASETHKAIQALNGRWFAGRKVVAEVYDQERFDNSDLSA

Target ORF information:

RefSeq Version	NM_001136033.2
Organism	Homo sapiens(human)
Definition	Homo sapiens poly(U) binding splicing factor 60 (PUF60), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001136033.2

ORF Insert Sequence:

ATGGAGAACG GGCAGAGCAC AGCCGCCAAG CTGGGGCTGC CTCCCCTGAC GCCCGAGCAG 
CAGGAGGCCC TTCAGAAGGC CAAGAAGTAC GCCATGGAGC AGAGCATCAA GAGTGTGCTG 
GTGAAGCAGA CCATCGCGCA CCAGCAGCAG CAGCTCACCA ACCTGCAGAT GGCAGCAGTG 
ACAATGGGCT TTGGAGATCC TCTCTCACCT TTGCAATCGA TGGCGGCTCA GCGGCAGCGG 
GCGCTGGCCA TCATGTGCCG CGTCTACGTG GGCTCTATCT ACTATGAGCT GGGGGAGGAC 
ACCATCCGCC AGGCCTTTGC CCCCTTTGGC CCCATCAAGA GCATCGACAT GTCCTGGGAC 
TCCGTCACCA TGAAGCACAA GGGCTTTGCC TTCGTGGAGT ATGAGGTCCC CGAAGCTGCA 
CAGCTGGCCT TGGAGCAGAT GAACTCGGTG ATGCTGGGGG GCAGGAACAT CAAGGTGGGC 
AGACCCAGCA ACATAGGGCA GGCCCAGCCC ATCATAGACC AGTTGGCTGA GGAGGCACGG 
GCCTTCAACC GCATCTACGT GGCCTCTGTG CACCAGGACC TCTCAGACGA TGACATCAAG 
AGCGTGTTTG AGGCCTTTGG CAAGATCAAG TCCTGCACAC TGGCCCGGGA CCCCACAACT 
GGCAAGCACA AGGGCTACGG CTTCATTGAG TACGAGAAGG CCCAGTCGTC CCAAGATGCT 
GTGTCTTCCA TGAACCTCTT TGACCTGGGT GGCCAGTACT TGCGGGTGGG CAAGGCTGTC 
ACACCGCCCA TGCCCCTACT CACACCAGCC ACGCCTGGAG GCCTCCCACC TGCCGCTGCT 
GTGGCAGCTG CTGCAGCCAC TGCCAAGATC ACAGCTCAGG AAGCAGTGGC CGGAGCAGCG 
GTGCTGGGTA CCCTGGGCAC ACCTGGACTG GTGTCCCCAG CACTGACCCT GGCCCAGCCC 
CTGGGCACTT TGCCCCAGGC TGTCATGGCT GCCCAGGCAC CTGGAGTCAT CACAGGTGTG 
ACCCCAGCCC GTCCTCCTAT CCCGGTCACC ATCCCCTCGG TGGGAGTGGT GAACCCCATC 
CTGGCCAGCC CTCCAACGCT GGGTCTCCTG GAGCCCAAGA AGGAGAAGGA AGAAGAGGAG 
CTGTTTCCCG AGTCAGAGCG GCCAGAGATG CTGAGCGAGC AGGAGCACAT GAGCATCTCG 
GGCAGTAGCG CCCGACACAT GGTGATGCAG AAGCTGCTCC GCAAGCAGGA GTCTACAGTG 
ATGGTTCTGC GCAACATGGT GGACCCCAAG GACATCGATG ATGACCTGGA AGGGGAGGTG 
ACAGAGGAGT GTGGCAAGTT CGGGGCCGTG AACCGCGTCA TCATCTACCA AGAGAAACAA 
GGCGAGGAGG AGGATGCAGA AATCATTGTC AAGATCTTTG TGGAGTTTTC CATAGCCTCT 
GAGACTCATA AGGCCATCCA GGCCCTCAAT GGCCGCTGGT TTGCTGGCCG CAAGGTGGTG 
GCTGAAGTGT ACGACCAGGA GCGTTTTGAT AACAGTGACC TCTCTGCGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PUF60 ( NM_001136033.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001129505.1 Homo sapiens poly(U) binding splicing factor 60 (PUF60), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu22582
Clone ID Related Accession (Same CDS sequence)	NM_001136033.2 , NM_001136033.3 , XM_017013239.1
Accession Version	NM_001136033.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1551bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-27
Organism	Homo sapiens(human)
Product	poly(U)-binding-splicing factor PUF60 isoform c
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BG771821.1, BC011265.1 and AW249619.1. On May 31, 2019 this sequence version replaced NM_001136033.2. Transcript Variant: This variant (3) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at an alternate in-frame downstream start site, compared to variant 1. The encoded isoform (c) has a shorter N-terminus, compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR3476690.1022935.1, SRR7410570.432098.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGAACG GGCAGAGCAC AGCCGCCAAG CTGGGGCTGC CTCCCCTGAC GCCCGAGCAG 
CAGGAGGCCC TTCAGAAGGC CAAGAAGTAC GCCATGGAGC AGAGCATCAA GAGTGTGCTG 
GTGAAGCAGA CCATCGCGCA CCAGCAGCAG CAGCTCACCA ACCTGCAGAT GGCAGCAGTG 
ACAATGGGCT TTGGAGATCC TCTCTCACCT TTGCAATCGA TGGCGGCTCA GCGGCAGCGG 
GCGCTGGCCA TCATGTGCCG CGTCTACGTG GGCTCTATCT ACTATGAGCT GGGGGAGGAC 
ACCATCCGCC AGGCCTTTGC CCCCTTTGGC CCCATCAAGA GCATCGACAT GTCCTGGGAC 
TCCGTCACCA TGAAGCACAA GGGCTTTGCC TTCGTGGAGT ATGAGGTCCC CGAAGCTGCA 
CAGCTGGCCT TGGAGCAGAT GAACTCGGTG ATGCTGGGGG GCAGGAACAT CAAGGTGGGC 
AGACCCAGCA ACATAGGGCA GGCCCAGCCC ATCATAGACC AGTTGGCTGA GGAGGCACGG 
GCCTTCAACC GCATCTACGT GGCCTCTGTG CACCAGGACC TCTCAGACGA TGACATCAAG 
AGCGTGTTTG AGGCCTTTGG CAAGATCAAG TCCTGCACAC TGGCCCGGGA CCCCACAACT 
GGCAAGCACA AGGGCTACGG CTTCATTGAG TACGAGAAGG CCCAGTCGTC CCAAGATGCT 
GTGTCTTCCA TGAACCTCTT TGACCTGGGT GGCCAGTACT TGCGGGTGGG CAAGGCTGTC 
ACACCGCCCA TGCCCCTACT CACACCAGCC ACGCCTGGAG GCCTCCCACC TGCCGCTGCT 
GTGGCAGCTG CTGCAGCCAC TGCCAAGATC ACAGCTCAGG AAGCAGTGGC CGGAGCAGCG 
GTGCTGGGTA CCCTGGGCAC ACCTGGACTG GTGTCCCCAG CACTGACCCT GGCCCAGCCC 
CTGGGCACTT TGCCCCAGGC TGTCATGGCT GCCCAGGCAC CTGGAGTCAT CACAGGTGTG 
ACCCCAGCCC GTCCTCCTAT CCCGGTCACC ATCCCCTCGG TGGGAGTGGT GAACCCCATC 
CTGGCCAGCC CTCCAACGCT GGGTCTCCTG GAGCCCAAGA AGGAGAAGGA AGAAGAGGAG 
CTGTTTCCCG AGTCAGAGCG GCCAGAGATG CTGAGCGAGC AGGAGCACAT GAGCATCTCG 
GGCAGTAGCG CCCGACACAT GGTGATGCAG AAGCTGCTCC GCAAGCAGGA GTCTACAGTG 
ATGGTTCTGC GCAACATGGT GGACCCCAAG GACATCGATG ATGACCTGGA AGGGGAGGTG 
ACAGAGGAGT GTGGCAAGTT CGGGGCCGTG AACCGCGTCA TCATCTACCA AGAGAAACAA 
GGCGAGGAGG AGGATGCAGA AATCATTGTC AAGATCTTTG TGGAGTTTTC CATAGCCTCT 
GAGACTCATA AGGCCATCCA GGCCCTCAAT GGCCGCTGGT TTGCTGGCCG CAAGGTGGTG 
GCTGAAGTGT ACGACCAGGA GCGTTTTGAT AACAGTGACC TCTCTGCGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001129505.1
CDS	164..1714
Translation	MENGQSTAAKLGLPPLTPEQQEALQKAKKYAMEQSIKSVLVKQTIAHQQQQLTNLQMAAVTMGFGDPLSPLQSMAAQRQRALAIMCRVYVGSIYYELGEDTIRQAFAPFGPIKSIDMSWDSVTMKHKGFAFVEYEVPEAAQLALEQMNSVMLGGRNIKVGRPSNIGQAQPIIDQLAEEARAFNRIYVASVHQDLSDDDIKSVFEAFGKIKSCTLARDPTTGKHKGYGFIEYEKAQSSQDAVSSMNLFDLGGQYLRVGKAVTPPMPLLTPATPGGLPPAAAVAAAAATAKITAQEAVAGAAVLGTLGTPGLVSPALTLAQPLGTLPQAVMAAQAPGVITGVTPARPPIPVTIPSVGVVNPILASPPTLGLLEPKKEKEEEELFPESERPEMLSEQEHMSISGSSARHMVMQKLLRKQESTVMVLRNMVDPKDIDDDLEGEVTEECGKFGAVNRVIIYQEKQGEEEDAEIIVKIFVEFSIASETHKAIQALNGRWFAGRKVVAEVYDQERFDNSDLSA

Target ORF information:

RefSeq Version	NM_001136033.3
Organism	Homo sapiens(human)
Definition	Homo sapiens poly(U) binding splicing factor 60 (PUF60), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001136033.3

ORF Insert Sequence:

ATGGAGAACG GGCAGAGCAC AGCCGCCAAG CTGGGGCTGC CTCCCCTGAC GCCCGAGCAG 
CAGGAGGCCC TTCAGAAGGC CAAGAAGTAC GCCATGGAGC AGAGCATCAA GAGTGTGCTG 
GTGAAGCAGA CCATCGCGCA CCAGCAGCAG CAGCTCACCA ACCTGCAGAT GGCAGCAGTG 
ACAATGGGCT TTGGAGATCC TCTCTCACCT TTGCAATCGA TGGCGGCTCA GCGGCAGCGG 
GCGCTGGCCA TCATGTGCCG CGTCTACGTG GGCTCTATCT ACTATGAGCT GGGGGAGGAC 
ACCATCCGCC AGGCCTTTGC CCCCTTTGGC CCCATCAAGA GCATCGACAT GTCCTGGGAC 
TCCGTCACCA TGAAGCACAA GGGCTTTGCC TTCGTGGAGT ATGAGGTCCC CGAAGCTGCA 
CAGCTGGCCT TGGAGCAGAT GAACTCGGTG ATGCTGGGGG GCAGGAACAT CAAGGTGGGC 
AGACCCAGCA ACATAGGGCA GGCCCAGCCC ATCATAGACC AGTTGGCTGA GGAGGCACGG 
GCCTTCAACC GCATCTACGT GGCCTCTGTG CACCAGGACC TCTCAGACGA TGACATCAAG 
AGCGTGTTTG AGGCCTTTGG CAAGATCAAG TCCTGCACAC TGGCCCGGGA CCCCACAACT 
GGCAAGCACA AGGGCTACGG CTTCATTGAG TACGAGAAGG CCCAGTCGTC CCAAGATGCT 
GTGTCTTCCA TGAACCTCTT TGACCTGGGT GGCCAGTACT TGCGGGTGGG CAAGGCTGTC 
ACACCGCCCA TGCCCCTACT CACACCAGCC ACGCCTGGAG GCCTCCCACC TGCCGCTGCT 
GTGGCAGCTG CTGCAGCCAC TGCCAAGATC ACAGCTCAGG AAGCAGTGGC CGGAGCAGCG 
GTGCTGGGTA CCCTGGGCAC ACCTGGACTG GTGTCCCCAG CACTGACCCT GGCCCAGCCC 
CTGGGCACTT TGCCCCAGGC TGTCATGGCT GCCCAGGCAC CTGGAGTCAT CACAGGTGTG 
ACCCCAGCCC GTCCTCCTAT CCCGGTCACC ATCCCCTCGG TGGGAGTGGT GAACCCCATC 
CTGGCCAGCC CTCCAACGCT GGGTCTCCTG GAGCCCAAGA AGGAGAAGGA AGAAGAGGAG 
CTGTTTCCCG AGTCAGAGCG GCCAGAGATG CTGAGCGAGC AGGAGCACAT GAGCATCTCG 
GGCAGTAGCG CCCGACACAT GGTGATGCAG AAGCTGCTCC GCAAGCAGGA GTCTACAGTG 
ATGGTTCTGC GCAACATGGT GGACCCCAAG GACATCGATG ATGACCTGGA AGGGGAGGTG 
ACAGAGGAGT GTGGCAAGTT CGGGGCCGTG AACCGCGTCA TCATCTACCA AGAGAAACAA 
GGCGAGGAGG AGGATGCAGA AATCATTGTC AAGATCTTTG TGGAGTTTTC CATAGCCTCT 
GAGACTCATA AGGCCATCCA GGCCCTCAAT GGCCGCTGGT TTGCTGGCCG CAAGGTGGTG 
GCTGAAGTGT ACGACCAGGA GCGTTTTGAT AACAGTGACC TCTCTGCGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PUF60 ( NM_001271100.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001258029.1 Homo sapiens poly(U) binding splicing factor 60 (PUF60), transcript variant 8, mRNA.

Price & Availability↑

CloneID	OHu23020
Clone ID Related Accession (Same CDS sequence)	NM_001271100.1 , NM_001271100.2 , XM_017013240.1
Accession Version	NM_001271100.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1500bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-10-20
Organism	Homo sapiens(human)
Product	poly(U)-binding-splicing factor PUF60 isoform h
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC234917.3, BG771821.1, BC009734.1 and AW249619.1. Transcript Variant: This variant (8) differs in the 5' UTR and contains multiple differences in the coding region, compared to variant 1. These differences result in initiation of translation at an alternate in-frame downstream start site. The encoded isoform (h) is shorter than isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC009734.1, SRR1803611.179234.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGAACG GGCAGAGCAC AGCCGCCAAG CTGGGGCTGC CTCCCCTGAC GCCCGAGCAG 
CAGGAGGCCC TTCAGAAGGC CAAGAAGTAC GCCATGGAGC AGAGCATCAA GAGTGTGCTG 
GTGAAGCAGA CCATCGCGCA CCAGCAGCAG CAGCTCACCA ACCTGCAGAT GGCGGCTCAG 
CGGCAGCGGG CGCTGGCCAT CATGTGCCGC GTCTACGTGG GCTCTATCTA CTATGAGCTG 
GGGGAGGACA CCATCCGCCA GGCCTTTGCC CCCTTTGGCC CCATCAAGAG CATCGACATG 
TCCTGGGACT CCGTCACCAT GAAGCACAAG GGCTTTGCCT TCGTGGAGTA TGAGGTCCCC 
GAAGCTGCAC AGCTGGCCTT GGAGCAGATG AACTCGGTGA TGCTGGGGGG CAGGAACATC 
AAGGTGGGCA GACCCAGCAA CATAGGGCAG GCCCAGCCCA TCATAGACCA GTTGGCTGAG 
GAGGCACGGG CCTTCAACCG CATCTACGTG GCCTCTGTGC ACCAGGACCT CTCAGACGAT 
GACATCAAGA GCGTGTTTGA GGCCTTTGGC AAGATCAAGT CCTGCACACT GGCCCGGGAC 
CCCACAACTG GCAAGCACAA GGGCTACGGC TTCATTGAGT ACGAGAAGGC CCAGTCGTCC 
CAAGATGCTG TGTCTTCCAT GAACCTCTTT GACCTGGGTG GCCAGTACTT GCGGGTGGGC 
AAGGCTGTCA CACCGCCCAT GCCCCTACTC ACACCAGCCA CGCCTGGAGG CCTCCCACCT 
GCCGCTGCTG TGGCAGCTGC TGCAGCCACT GCCAAGATCA CAGCTCAGGA AGCAGTGGCC 
GGAGCAGCGG TGCTGGGTAC CCTGGGCACA CCTGGACTGG TGTCCCCAGC ACTGACCCTG 
GCCCAGCCCC TGGGCACTTT GCCCCAGGCT GTCATGGCTG CCCAGGCACC TGGAGTCATC 
ACAGGTGTGA CCCCAGCCCG TCCTCCTATC CCGGTCACCA TCCCCTCGGT GGGAGTGGTG 
AACCCCATCC TGGCCAGCCC TCCAACGCTG GGTCTCCTGG AGCCCAAGAA GGAGAAGGAA 
GAAGAGGAGC TGTTTCCCGA GTCAGAGCGG CCAGAGATGC TGAGCGAGCA GGAGCACATG 
AGCATCTCGG GCAGTAGCGC CCGACACATG GTGATGCAGA AGCTGCTCCG CAAGCAGGAG 
TCTACAGTGA TGGTTCTGCG CAACATGGTG GACCCCAAGG ACATCGATGA TGACCTGGAA 
GGGGAGGTGA CAGAGGAGTG TGGCAAGTTC GGGGCCGTGA ACCGCGTCAT CATCTACCAA 
GAGAAACAAG GCGAGGAGGA GGATGCAGAA ATCATTGTCA AGATCTTTGT GGAGTTTTCC 
ATAGCCTCTG AGACTCATAA GGCCATCCAG GCCCTCAATG GCCGCTGGTT TGCTGGCCGC 
AAGGTGGTGG CTGAAGTGTA CGACCAGGAG CGTTTTGATA ACAGTGACCT CTCTGCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001258029.1
CDS	232..1731
Translation	MENGQSTAAKLGLPPLTPEQQEALQKAKKYAMEQSIKSVLVKQTIAHQQQQLTNLQMAAQRQRALAIMCRVYVGSIYYELGEDTIRQAFAPFGPIKSIDMSWDSVTMKHKGFAFVEYEVPEAAQLALEQMNSVMLGGRNIKVGRPSNIGQAQPIIDQLAEEARAFNRIYVASVHQDLSDDDIKSVFEAFGKIKSCTLARDPTTGKHKGYGFIEYEKAQSSQDAVSSMNLFDLGGQYLRVGKAVTPPMPLLTPATPGGLPPAAAVAAAAATAKITAQEAVAGAAVLGTLGTPGLVSPALTLAQPLGTLPQAVMAAQAPGVITGVTPARPPIPVTIPSVGVVNPILASPPTLGLLEPKKEKEEEELFPESERPEMLSEQEHMSISGSSARHMVMQKLLRKQESTVMVLRNMVDPKDIDDDLEGEVTEECGKFGAVNRVIIYQEKQGEEEDAEIIVKIFVEFSIASETHKAIQALNGRWFAGRKVVAEVYDQERFDNSDLSA

Target ORF information:

RefSeq Version	NM_001271100.1
Organism	Homo sapiens(human)
Definition	Homo sapiens poly(U) binding splicing factor 60 (PUF60), transcript variant 8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001271100.1

ORF Insert Sequence:

ATGGAGAACG GGCAGAGCAC AGCCGCCAAG CTGGGGCTGC CTCCCCTGAC GCCCGAGCAG 
CAGGAGGCCC TTCAGAAGGC CAAGAAGTAC GCCATGGAGC AGAGCATCAA GAGTGTGCTG 
GTGAAGCAGA CCATCGCGCA CCAGCAGCAG CAGCTCACCA ACCTGCAGAT GGCGGCTCAG 
CGGCAGCGGG CGCTGGCCAT CATGTGCCGC GTCTACGTGG GCTCTATCTA CTATGAGCTG 
GGGGAGGACA CCATCCGCCA GGCCTTTGCC CCCTTTGGCC CCATCAAGAG CATCGACATG 
TCCTGGGACT CCGTCACCAT GAAGCACAAG GGCTTTGCCT TCGTGGAGTA TGAGGTCCCC 
GAAGCTGCAC AGCTGGCCTT GGAGCAGATG AACTCGGTGA TGCTGGGGGG CAGGAACATC 
AAGGTGGGCA GACCCAGCAA CATAGGGCAG GCCCAGCCCA TCATAGACCA GTTGGCTGAG 
GAGGCACGGG CCTTCAACCG CATCTACGTG GCCTCTGTGC ACCAGGACCT CTCAGACGAT 
GACATCAAGA GCGTGTTTGA GGCCTTTGGC AAGATCAAGT CCTGCACACT GGCCCGGGAC 
CCCACAACTG GCAAGCACAA GGGCTACGGC TTCATTGAGT ACGAGAAGGC CCAGTCGTCC 
CAAGATGCTG TGTCTTCCAT GAACCTCTTT GACCTGGGTG GCCAGTACTT GCGGGTGGGC 
AAGGCTGTCA CACCGCCCAT GCCCCTACTC ACACCAGCCA CGCCTGGAGG CCTCCCACCT 
GCCGCTGCTG TGGCAGCTGC TGCAGCCACT GCCAAGATCA CAGCTCAGGA AGCAGTGGCC 
GGAGCAGCGG TGCTGGGTAC CCTGGGCACA CCTGGACTGG TGTCCCCAGC ACTGACCCTG 
GCCCAGCCCC TGGGCACTTT GCCCCAGGCT GTCATGGCTG CCCAGGCACC TGGAGTCATC 
ACAGGTGTGA CCCCAGCCCG TCCTCCTATC CCGGTCACCA TCCCCTCGGT GGGAGTGGTG 
AACCCCATCC TGGCCAGCCC TCCAACGCTG GGTCTCCTGG AGCCCAAGAA GGAGAAGGAA 
GAAGAGGAGC TGTTTCCCGA GTCAGAGCGG CCAGAGATGC TGAGCGAGCA GGAGCACATG 
AGCATCTCGG GCAGTAGCGC CCGACACATG GTGATGCAGA AGCTGCTCCG CAAGCAGGAG 
TCTACAGTGA TGGTTCTGCG CAACATGGTG GACCCCAAGG ACATCGATGA TGACCTGGAA 
GGGGAGGTGA CAGAGGAGTG TGGCAAGTTC GGGGCCGTGA ACCGCGTCAT CATCTACCAA 
GAGAAACAAG GCGAGGAGGA GGATGCAGAA ATCATTGTCA AGATCTTTGT GGAGTTTTCC 
ATAGCCTCTG AGACTCATAA GGCCATCCAG GCCCTCAATG GCCGCTGGTT TGCTGGCCGC 
AAGGTGGTGG CTGAAGTGTA CGACCAGGAG CGTTTTGATA ACAGTGACCT CTCTGCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PUF60 ( NM_001271100.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001258029.1 Homo sapiens poly(U) binding splicing factor 60 (PUF60), transcript variant 8, mRNA.

Price & Availability↑

CloneID	OHu23020
Clone ID Related Accession (Same CDS sequence)	NM_001271100.1 , NM_001271100.2 , XM_017013240.1
Accession Version	NM_001271100.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1500bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-27
Organism	Homo sapiens(human)
Product	poly(U)-binding-splicing factor PUF60 isoform h
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BG771821.1, BC009734.1 and AW249619.1. On May 31, 2019 this sequence version replaced NM_001271100.1. Transcript Variant: This variant (8) differs in the 5' UTR and contains multiple differences in the coding region, compared to variant 1. These differences result in initiation of translation at an alternate in-frame downstream start site. The encoded isoform (h) is shorter than isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR7410570.432094.1, BC009734.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGAACG GGCAGAGCAC AGCCGCCAAG CTGGGGCTGC CTCCCCTGAC GCCCGAGCAG 
CAGGAGGCCC TTCAGAAGGC CAAGAAGTAC GCCATGGAGC AGAGCATCAA GAGTGTGCTG 
GTGAAGCAGA CCATCGCGCA CCAGCAGCAG CAGCTCACCA ACCTGCAGAT GGCGGCTCAG 
CGGCAGCGGG CGCTGGCCAT CATGTGCCGC GTCTACGTGG GCTCTATCTA CTATGAGCTG 
GGGGAGGACA CCATCCGCCA GGCCTTTGCC CCCTTTGGCC CCATCAAGAG CATCGACATG 
TCCTGGGACT CCGTCACCAT GAAGCACAAG GGCTTTGCCT TCGTGGAGTA TGAGGTCCCC 
GAAGCTGCAC AGCTGGCCTT GGAGCAGATG AACTCGGTGA TGCTGGGGGG CAGGAACATC 
AAGGTGGGCA GACCCAGCAA CATAGGGCAG GCCCAGCCCA TCATAGACCA GTTGGCTGAG 
GAGGCACGGG CCTTCAACCG CATCTACGTG GCCTCTGTGC ACCAGGACCT CTCAGACGAT 
GACATCAAGA GCGTGTTTGA GGCCTTTGGC AAGATCAAGT CCTGCACACT GGCCCGGGAC 
CCCACAACTG GCAAGCACAA GGGCTACGGC TTCATTGAGT ACGAGAAGGC CCAGTCGTCC 
CAAGATGCTG TGTCTTCCAT GAACCTCTTT GACCTGGGTG GCCAGTACTT GCGGGTGGGC 
AAGGCTGTCA CACCGCCCAT GCCCCTACTC ACACCAGCCA CGCCTGGAGG CCTCCCACCT 
GCCGCTGCTG TGGCAGCTGC TGCAGCCACT GCCAAGATCA CAGCTCAGGA AGCAGTGGCC 
GGAGCAGCGG TGCTGGGTAC CCTGGGCACA CCTGGACTGG TGTCCCCAGC ACTGACCCTG 
GCCCAGCCCC TGGGCACTTT GCCCCAGGCT GTCATGGCTG CCCAGGCACC TGGAGTCATC 
ACAGGTGTGA CCCCAGCCCG TCCTCCTATC CCGGTCACCA TCCCCTCGGT GGGAGTGGTG 
AACCCCATCC TGGCCAGCCC TCCAACGCTG GGTCTCCTGG AGCCCAAGAA GGAGAAGGAA 
GAAGAGGAGC TGTTTCCCGA GTCAGAGCGG CCAGAGATGC TGAGCGAGCA GGAGCACATG 
AGCATCTCGG GCAGTAGCGC CCGACACATG GTGATGCAGA AGCTGCTCCG CAAGCAGGAG 
TCTACAGTGA TGGTTCTGCG CAACATGGTG GACCCCAAGG ACATCGATGA TGACCTGGAA 
GGGGAGGTGA CAGAGGAGTG TGGCAAGTTC GGGGCCGTGA ACCGCGTCAT CATCTACCAA 
GAGAAACAAG GCGAGGAGGA GGATGCAGAA ATCATTGTCA AGATCTTTGT GGAGTTTTCC 
ATAGCCTCTG AGACTCATAA GGCCATCCAG GCCCTCAATG GCCGCTGGTT TGCTGGCCGC 
AAGGTGGTGG CTGAAGTGTA CGACCAGGAG CGTTTTGATA ACAGTGACCT CTCTGCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001258029.1
CDS	164..1663
Translation	MENGQSTAAKLGLPPLTPEQQEALQKAKKYAMEQSIKSVLVKQTIAHQQQQLTNLQMAAQRQRALAIMCRVYVGSIYYELGEDTIRQAFAPFGPIKSIDMSWDSVTMKHKGFAFVEYEVPEAAQLALEQMNSVMLGGRNIKVGRPSNIGQAQPIIDQLAEEARAFNRIYVASVHQDLSDDDIKSVFEAFGKIKSCTLARDPTTGKHKGYGFIEYEKAQSSQDAVSSMNLFDLGGQYLRVGKAVTPPMPLLTPATPGGLPPAAAVAAAAATAKITAQEAVAGAAVLGTLGTPGLVSPALTLAQPLGTLPQAVMAAQAPGVITGVTPARPPIPVTIPSVGVVNPILASPPTLGLLEPKKEKEEEELFPESERPEMLSEQEHMSISGSSARHMVMQKLLRKQESTVMVLRNMVDPKDIDDDLEGEVTEECGKFGAVNRVIIYQEKQGEEEDAEIIVKIFVEFSIASETHKAIQALNGRWFAGRKVVAEVYDQERFDNSDLSA

Target ORF information:

RefSeq Version	NM_001271100.2
Organism	Homo sapiens(human)
Definition	Homo sapiens poly(U) binding splicing factor 60 (PUF60), transcript variant 8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001271100.2

ORF Insert Sequence:

ATGGAGAACG GGCAGAGCAC AGCCGCCAAG CTGGGGCTGC CTCCCCTGAC GCCCGAGCAG 
CAGGAGGCCC TTCAGAAGGC CAAGAAGTAC GCCATGGAGC AGAGCATCAA GAGTGTGCTG 
GTGAAGCAGA CCATCGCGCA CCAGCAGCAG CAGCTCACCA ACCTGCAGAT GGCGGCTCAG 
CGGCAGCGGG CGCTGGCCAT CATGTGCCGC GTCTACGTGG GCTCTATCTA CTATGAGCTG 
GGGGAGGACA CCATCCGCCA GGCCTTTGCC CCCTTTGGCC CCATCAAGAG CATCGACATG 
TCCTGGGACT CCGTCACCAT GAAGCACAAG GGCTTTGCCT TCGTGGAGTA TGAGGTCCCC 
GAAGCTGCAC AGCTGGCCTT GGAGCAGATG AACTCGGTGA TGCTGGGGGG CAGGAACATC 
AAGGTGGGCA GACCCAGCAA CATAGGGCAG GCCCAGCCCA TCATAGACCA GTTGGCTGAG 
GAGGCACGGG CCTTCAACCG CATCTACGTG GCCTCTGTGC ACCAGGACCT CTCAGACGAT 
GACATCAAGA GCGTGTTTGA GGCCTTTGGC AAGATCAAGT CCTGCACACT GGCCCGGGAC 
CCCACAACTG GCAAGCACAA GGGCTACGGC TTCATTGAGT ACGAGAAGGC CCAGTCGTCC 
CAAGATGCTG TGTCTTCCAT GAACCTCTTT GACCTGGGTG GCCAGTACTT GCGGGTGGGC 
AAGGCTGTCA CACCGCCCAT GCCCCTACTC ACACCAGCCA CGCCTGGAGG CCTCCCACCT 
GCCGCTGCTG TGGCAGCTGC TGCAGCCACT GCCAAGATCA CAGCTCAGGA AGCAGTGGCC 
GGAGCAGCGG TGCTGGGTAC CCTGGGCACA CCTGGACTGG TGTCCCCAGC ACTGACCCTG 
GCCCAGCCCC TGGGCACTTT GCCCCAGGCT GTCATGGCTG CCCAGGCACC TGGAGTCATC 
ACAGGTGTGA CCCCAGCCCG TCCTCCTATC CCGGTCACCA TCCCCTCGGT GGGAGTGGTG 
AACCCCATCC TGGCCAGCCC TCCAACGCTG GGTCTCCTGG AGCCCAAGAA GGAGAAGGAA 
GAAGAGGAGC TGTTTCCCGA GTCAGAGCGG CCAGAGATGC TGAGCGAGCA GGAGCACATG 
AGCATCTCGG GCAGTAGCGC CCGACACATG GTGATGCAGA AGCTGCTCCG CAAGCAGGAG 
TCTACAGTGA TGGTTCTGCG CAACATGGTG GACCCCAAGG ACATCGATGA TGACCTGGAA 
GGGGAGGTGA CAGAGGAGTG TGGCAAGTTC GGGGCCGTGA ACCGCGTCAT CATCTACCAA 
GAGAAACAAG GCGAGGAGGA GGATGCAGAA ATCATTGTCA AGATCTTTGT GGAGTTTTCC 
ATAGCCTCTG AGACTCATAA GGCCATCCAG GCCCTCAATG GCCGCTGGTT TGCTGGCCGC 
AAGGTGGTGG CTGAAGTGTA CGACCAGGAG CGTTTTGATA ACAGTGACCT CTCTGCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PUF60 ( XM_017013239.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016868728.1 Homo sapiens poly(U) binding splicing factor 60 (PUF60), transcript variant X17, mRNA.

Price & Availability↑

CloneID	OHu22582
Clone ID Related Accession (Same CDS sequence)	NM_001136033.2 , NM_001136033.3 , XM_017013239.1
Accession Version	XM_017013239.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1551bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	poly(U)-binding-splicing factor PUF60 isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000008.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAACG GGCAGAGCAC AGCCGCCAAG CTGGGGCTGC CTCCCCTGAC GCCCGAGCAG 
CAGGAGGCCC TTCAGAAGGC CAAGAAGTAC GCCATGGAGC AGAGCATCAA GAGTGTGCTG 
GTGAAGCAGA CCATCGCGCA CCAGCAGCAG CAGCTCACCA ACCTGCAGAT GGCAGCAGTG 
ACAATGGGCT TTGGAGATCC TCTCTCACCT TTGCAATCGA TGGCGGCTCA GCGGCAGCGG 
GCGCTGGCCA TCATGTGCCG CGTCTACGTG GGCTCTATCT ACTATGAGCT GGGGGAGGAC 
ACCATCCGCC AGGCCTTTGC CCCCTTTGGC CCCATCAAGA GCATCGACAT GTCCTGGGAC 
TCCGTCACCA TGAAGCACAA GGGCTTTGCC TTCGTGGAGT ATGAGGTCCC CGAAGCTGCA 
CAGCTGGCCT TGGAGCAGAT GAACTCGGTG ATGCTGGGGG GCAGGAACAT CAAGGTGGGC 
AGACCCAGCA ACATAGGGCA GGCCCAGCCC ATCATAGACC AGTTGGCTGA GGAGGCACGG 
GCCTTCAACC GCATCTACGT GGCCTCTGTG CACCAGGACC TCTCAGACGA TGACATCAAG 
AGCGTGTTTG AGGCCTTTGG CAAGATCAAG TCCTGCACAC TGGCCCGGGA CCCCACAACT 
GGCAAGCACA AGGGCTACGG CTTCATTGAG TACGAGAAGG CCCAGTCGTC CCAAGATGCT 
GTGTCTTCCA TGAACCTCTT TGACCTGGGT GGCCAGTACT TGCGGGTGGG CAAGGCTGTC 
ACACCGCCCA TGCCCCTACT CACACCAGCC ACGCCTGGAG GCCTCCCACC TGCCGCTGCT 
GTGGCAGCTG CTGCAGCCAC TGCCAAGATC ACAGCTCAGG AAGCAGTGGC CGGAGCAGCG 
GTGCTGGGTA CCCTGGGCAC ACCTGGACTG GTGTCCCCAG CACTGACCCT GGCCCAGCCC 
CTGGGCACTT TGCCCCAGGC TGTCATGGCT GCCCAGGCAC CTGGAGTCAT CACAGGTGTG 
ACCCCAGCCC GTCCTCCTAT CCCGGTCACC ATCCCCTCGG TGGGAGTGGT GAACCCCATC 
CTGGCCAGCC CTCCAACGCT GGGTCTCCTG GAGCCCAAGA AGGAGAAGGA AGAAGAGGAG 
CTGTTTCCCG AGTCAGAGCG GCCAGAGATG CTGAGCGAGC AGGAGCACAT GAGCATCTCG 
GGCAGTAGCG CCCGACACAT GGTGATGCAG AAGCTGCTCC GCAAGCAGGA GTCTACAGTG 
ATGGTTCTGC GCAACATGGT GGACCCCAAG GACATCGATG ATGACCTGGA AGGGGAGGTG 
ACAGAGGAGT GTGGCAAGTT CGGGGCCGTG AACCGCGTCA TCATCTACCA AGAGAAACAA 
GGCGAGGAGG AGGATGCAGA AATCATTGTC AAGATCTTTG TGGAGTTTTC CATAGCCTCT 
GAGACTCATA AGGCCATCCA GGCCCTCAAT GGCCGCTGGT TTGCTGGCCG CAAGGTGGTG 
GCTGAAGTGT ACGACCAGGA GCGTTTTGAT AACAGTGACC TCTCTGCGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016868728.1
CDS	240..1790
Translation	MENGQSTAAKLGLPPLTPEQQEALQKAKKYAMEQSIKSVLVKQTIAHQQQQLTNLQMAAVTMGFGDPLSPLQSMAAQRQRALAIMCRVYVGSIYYELGEDTIRQAFAPFGPIKSIDMSWDSVTMKHKGFAFVEYEVPEAAQLALEQMNSVMLGGRNIKVGRPSNIGQAQPIIDQLAEEARAFNRIYVASVHQDLSDDDIKSVFEAFGKIKSCTLARDPTTGKHKGYGFIEYEKAQSSQDAVSSMNLFDLGGQYLRVGKAVTPPMPLLTPATPGGLPPAAAVAAAAATAKITAQEAVAGAAVLGTLGTPGLVSPALTLAQPLGTLPQAVMAAQAPGVITGVTPARPPIPVTIPSVGVVNPILASPPTLGLLEPKKEKEEEELFPESERPEMLSEQEHMSISGSSARHMVMQKLLRKQESTVMVLRNMVDPKDIDDDLEGEVTEECGKFGAVNRVIIYQEKQGEEEDAEIIVKIFVEFSIASETHKAIQALNGRWFAGRKVVAEVYDQERFDNSDLSA

Target ORF information:

RefSeq Version	XM_017013239.1
Organism	Homo sapiens(human)
Definition	Homo sapiens poly(U) binding splicing factor 60 (PUF60), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017013239.1

ORF Insert Sequence:

ATGGAGAACG GGCAGAGCAC AGCCGCCAAG CTGGGGCTGC CTCCCCTGAC GCCCGAGCAG 
CAGGAGGCCC TTCAGAAGGC CAAGAAGTAC GCCATGGAGC AGAGCATCAA GAGTGTGCTG 
GTGAAGCAGA CCATCGCGCA CCAGCAGCAG CAGCTCACCA ACCTGCAGAT GGCAGCAGTG 
ACAATGGGCT TTGGAGATCC TCTCTCACCT TTGCAATCGA TGGCGGCTCA GCGGCAGCGG 
GCGCTGGCCA TCATGTGCCG CGTCTACGTG GGCTCTATCT ACTATGAGCT GGGGGAGGAC 
ACCATCCGCC AGGCCTTTGC CCCCTTTGGC CCCATCAAGA GCATCGACAT GTCCTGGGAC 
TCCGTCACCA TGAAGCACAA GGGCTTTGCC TTCGTGGAGT ATGAGGTCCC CGAAGCTGCA 
CAGCTGGCCT TGGAGCAGAT GAACTCGGTG ATGCTGGGGG GCAGGAACAT CAAGGTGGGC 
AGACCCAGCA ACATAGGGCA GGCCCAGCCC ATCATAGACC AGTTGGCTGA GGAGGCACGG 
GCCTTCAACC GCATCTACGT GGCCTCTGTG CACCAGGACC TCTCAGACGA TGACATCAAG 
AGCGTGTTTG AGGCCTTTGG CAAGATCAAG TCCTGCACAC TGGCCCGGGA CCCCACAACT 
GGCAAGCACA AGGGCTACGG CTTCATTGAG TACGAGAAGG CCCAGTCGTC CCAAGATGCT 
GTGTCTTCCA TGAACCTCTT TGACCTGGGT GGCCAGTACT TGCGGGTGGG CAAGGCTGTC 
ACACCGCCCA TGCCCCTACT CACACCAGCC ACGCCTGGAG GCCTCCCACC TGCCGCTGCT 
GTGGCAGCTG CTGCAGCCAC TGCCAAGATC ACAGCTCAGG AAGCAGTGGC CGGAGCAGCG 
GTGCTGGGTA CCCTGGGCAC ACCTGGACTG GTGTCCCCAG CACTGACCCT GGCCCAGCCC 
CTGGGCACTT TGCCCCAGGC TGTCATGGCT GCCCAGGCAC CTGGAGTCAT CACAGGTGTG 
ACCCCAGCCC GTCCTCCTAT CCCGGTCACC ATCCCCTCGG TGGGAGTGGT GAACCCCATC 
CTGGCCAGCC CTCCAACGCT GGGTCTCCTG GAGCCCAAGA AGGAGAAGGA AGAAGAGGAG 
CTGTTTCCCG AGTCAGAGCG GCCAGAGATG CTGAGCGAGC AGGAGCACAT GAGCATCTCG 
GGCAGTAGCG CCCGACACAT GGTGATGCAG AAGCTGCTCC GCAAGCAGGA GTCTACAGTG 
ATGGTTCTGC GCAACATGGT GGACCCCAAG GACATCGATG ATGACCTGGA AGGGGAGGTG 
ACAGAGGAGT GTGGCAAGTT CGGGGCCGTG AACCGCGTCA TCATCTACCA AGAGAAACAA 
GGCGAGGAGG AGGATGCAGA AATCATTGTC AAGATCTTTG TGGAGTTTTC CATAGCCTCT 
GAGACTCATA AGGCCATCCA GGCCCTCAAT GGCCGCTGGT TTGCTGGCCG CAAGGTGGTG 
GCTGAAGTGT ACGACCAGGA GCGTTTTGAT AACAGTGACC TCTCTGCGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PUF60 ( XM_017013240.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016868729.1 Homo sapiens poly(U) binding splicing factor 60 (PUF60), transcript variant X18, mRNA.

Price & Availability↑

CloneID	OHu23020
Clone ID Related Accession (Same CDS sequence)	NM_001271100.1 , NM_001271100.2 , XM_017013240.1
Accession Version	XM_017013240.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1500bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	poly(U)-binding-splicing factor PUF60 isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000008.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAACG GGCAGAGCAC AGCCGCCAAG CTGGGGCTGC CTCCCCTGAC GCCCGAGCAG 
CAGGAGGCCC TTCAGAAGGC CAAGAAGTAC GCCATGGAGC AGAGCATCAA GAGTGTGCTG 
GTGAAGCAGA CCATCGCGCA CCAGCAGCAG CAGCTCACCA ACCTGCAGAT GGCGGCTCAG 
CGGCAGCGGG CGCTGGCCAT CATGTGCCGC GTCTACGTGG GCTCTATCTA CTATGAGCTG 
GGGGAGGACA CCATCCGCCA GGCCTTTGCC CCCTTTGGCC CCATCAAGAG CATCGACATG 
TCCTGGGACT CCGTCACCAT GAAGCACAAG GGCTTTGCCT TCGTGGAGTA TGAGGTCCCC 
GAAGCTGCAC AGCTGGCCTT GGAGCAGATG AACTCGGTGA TGCTGGGGGG CAGGAACATC 
AAGGTGGGCA GACCCAGCAA CATAGGGCAG GCCCAGCCCA TCATAGACCA GTTGGCTGAG 
GAGGCACGGG CCTTCAACCG CATCTACGTG GCCTCTGTGC ACCAGGACCT CTCAGACGAT 
GACATCAAGA GCGTGTTTGA GGCCTTTGGC AAGATCAAGT CCTGCACACT GGCCCGGGAC 
CCCACAACTG GCAAGCACAA GGGCTACGGC TTCATTGAGT ACGAGAAGGC CCAGTCGTCC 
CAAGATGCTG TGTCTTCCAT GAACCTCTTT GACCTGGGTG GCCAGTACTT GCGGGTGGGC 
AAGGCTGTCA CACCGCCCAT GCCCCTACTC ACACCAGCCA CGCCTGGAGG CCTCCCACCT 
GCCGCTGCTG TGGCAGCTGC TGCAGCCACT GCCAAGATCA CAGCTCAGGA AGCAGTGGCC 
GGAGCAGCGG TGCTGGGTAC CCTGGGCACA CCTGGACTGG TGTCCCCAGC ACTGACCCTG 
GCCCAGCCCC TGGGCACTTT GCCCCAGGCT GTCATGGCTG CCCAGGCACC TGGAGTCATC 
ACAGGTGTGA CCCCAGCCCG TCCTCCTATC CCGGTCACCA TCCCCTCGGT GGGAGTGGTG 
AACCCCATCC TGGCCAGCCC TCCAACGCTG GGTCTCCTGG AGCCCAAGAA GGAGAAGGAA 
GAAGAGGAGC TGTTTCCCGA GTCAGAGCGG CCAGAGATGC TGAGCGAGCA GGAGCACATG 
AGCATCTCGG GCAGTAGCGC CCGACACATG GTGATGCAGA AGCTGCTCCG CAAGCAGGAG 
TCTACAGTGA TGGTTCTGCG CAACATGGTG GACCCCAAGG ACATCGATGA TGACCTGGAA 
GGGGAGGTGA CAGAGGAGTG TGGCAAGTTC GGGGCCGTGA ACCGCGTCAT CATCTACCAA 
GAGAAACAAG GCGAGGAGGA GGATGCAGAA ATCATTGTCA AGATCTTTGT GGAGTTTTCC 
ATAGCCTCTG AGACTCATAA GGCCATCCAG GCCCTCAATG GCCGCTGGTT TGCTGGCCGC 
AAGGTGGTGG CTGAAGTGTA CGACCAGGAG CGTTTTGATA ACAGTGACCT CTCTGCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016868729.1
CDS	240..1739
Translation	MENGQSTAAKLGLPPLTPEQQEALQKAKKYAMEQSIKSVLVKQTIAHQQQQLTNLQMAAQRQRALAIMCRVYVGSIYYELGEDTIRQAFAPFGPIKSIDMSWDSVTMKHKGFAFVEYEVPEAAQLALEQMNSVMLGGRNIKVGRPSNIGQAQPIIDQLAEEARAFNRIYVASVHQDLSDDDIKSVFEAFGKIKSCTLARDPTTGKHKGYGFIEYEKAQSSQDAVSSMNLFDLGGQYLRVGKAVTPPMPLLTPATPGGLPPAAAVAAAAATAKITAQEAVAGAAVLGTLGTPGLVSPALTLAQPLGTLPQAVMAAQAPGVITGVTPARPPIPVTIPSVGVVNPILASPPTLGLLEPKKEKEEEELFPESERPEMLSEQEHMSISGSSARHMVMQKLLRKQESTVMVLRNMVDPKDIDDDLEGEVTEECGKFGAVNRVIIYQEKQGEEEDAEIIVKIFVEFSIASETHKAIQALNGRWFAGRKVVAEVYDQERFDNSDLSA

Target ORF information:

RefSeq Version	XM_017013240.1
Organism	Homo sapiens(human)
Definition	Homo sapiens poly(U) binding splicing factor 60 (PUF60), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017013240.1

ORF Insert Sequence:

ATGGAGAACG GGCAGAGCAC AGCCGCCAAG CTGGGGCTGC CTCCCCTGAC GCCCGAGCAG 
CAGGAGGCCC TTCAGAAGGC CAAGAAGTAC GCCATGGAGC AGAGCATCAA GAGTGTGCTG 
GTGAAGCAGA CCATCGCGCA CCAGCAGCAG CAGCTCACCA ACCTGCAGAT GGCGGCTCAG 
CGGCAGCGGG CGCTGGCCAT CATGTGCCGC GTCTACGTGG GCTCTATCTA CTATGAGCTG 
GGGGAGGACA CCATCCGCCA GGCCTTTGCC CCCTTTGGCC CCATCAAGAG CATCGACATG 
TCCTGGGACT CCGTCACCAT GAAGCACAAG GGCTTTGCCT TCGTGGAGTA TGAGGTCCCC 
GAAGCTGCAC AGCTGGCCTT GGAGCAGATG AACTCGGTGA TGCTGGGGGG CAGGAACATC 
AAGGTGGGCA GACCCAGCAA CATAGGGCAG GCCCAGCCCA TCATAGACCA GTTGGCTGAG 
GAGGCACGGG CCTTCAACCG CATCTACGTG GCCTCTGTGC ACCAGGACCT CTCAGACGAT 
GACATCAAGA GCGTGTTTGA GGCCTTTGGC AAGATCAAGT CCTGCACACT GGCCCGGGAC 
CCCACAACTG GCAAGCACAA GGGCTACGGC TTCATTGAGT ACGAGAAGGC CCAGTCGTCC 
CAAGATGCTG TGTCTTCCAT GAACCTCTTT GACCTGGGTG GCCAGTACTT GCGGGTGGGC 
AAGGCTGTCA CACCGCCCAT GCCCCTACTC ACACCAGCCA CGCCTGGAGG CCTCCCACCT 
GCCGCTGCTG TGGCAGCTGC TGCAGCCACT GCCAAGATCA CAGCTCAGGA AGCAGTGGCC 
GGAGCAGCGG TGCTGGGTAC CCTGGGCACA CCTGGACTGG TGTCCCCAGC ACTGACCCTG 
GCCCAGCCCC TGGGCACTTT GCCCCAGGCT GTCATGGCTG CCCAGGCACC TGGAGTCATC 
ACAGGTGTGA CCCCAGCCCG TCCTCCTATC CCGGTCACCA TCCCCTCGGT GGGAGTGGTG 
AACCCCATCC TGGCCAGCCC TCCAACGCTG GGTCTCCTGG AGCCCAAGAA GGAGAAGGAA 
GAAGAGGAGC TGTTTCCCGA GTCAGAGCGG CCAGAGATGC TGAGCGAGCA GGAGCACATG 
AGCATCTCGG GCAGTAGCGC CCGACACATG GTGATGCAGA AGCTGCTCCG CAAGCAGGAG 
TCTACAGTGA TGGTTCTGCG CAACATGGTG GACCCCAAGG ACATCGATGA TGACCTGGAA 
GGGGAGGTGA CAGAGGAGTG TGGCAAGTTC GGGGCCGTGA ACCGCGTCAT CATCTACCAA 
GAGAAACAAG GCGAGGAGGA GGATGCAGAA ATCATTGTCA AGATCTTTGT GGAGTTTTCC 
ATAGCCTCTG AGACTCATAA GGCCATCCAG GCCCTCAATG GCCGCTGGTT TGCTGGCCGC 
AAGGTGGTGG CTGAAGTGTA CGACCAGGAG CGTTTTGATA ACAGTGACCT CTCTGCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PUF60 ( NM_078480.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_510965.1 Homo sapiens poly(U) binding splicing factor 60 (PUF60), transcript variant 1, mRNA.

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CloneID	OHu20314
Clone ID Related Accession (Same CDS sequence)	NM_078480.2 , NM_078480.3
Accession Version	NM_078480.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1680bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-22
Organism	Homo sapiens(human)
Product	poly(U)-binding-splicing factor PUF60 isoform a
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CN371576.1, AF114818.1, AF217197.2 and AW249619.1. This sequence is a reference standard in the RefSeqGene project. On Sep 1, 2012 this sequence version replaced NM_078480.1. Transcript Variant: This variant (1) encodes the isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF114818.1, SRR1803613.125708.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGACGG CGACCATAGC TCTCCAGGTC AATGGCCAGC AAGGAGGGGG GTCCGAGCCG 
GCGGCGGCGG CGGCAGTGGT GGCAGCGGGA GACAAATGGA AACCTCCACA GGGCACAGAC 
TCCATCAAGA TGGAGAACGG GCAGAGCACA GCCGCCAAGC TGGGGCTGCC TCCCCTGACG 
CCCGAGCAGC AGGAGGCCCT TCAGAAGGCC AAGAAGTACG CCATGGAGCA GAGCATCAAG 
AGTGTGCTGG TGAAGCAGAC CATCGCGCAC CAGCAGCAGC AGCTCACCAA CCTGCAGATG 
GCAGCAGTGA CAATGGGCTT TGGAGATCCT CTCTCACCTT TGCAATCGAT GGCGGCTCAG 
CGGCAGCGGG CGCTGGCCAT CATGTGCCGC GTCTACGTGG GCTCTATCTA CTATGAGCTG 
GGGGAGGACA CCATCCGCCA GGCCTTTGCC CCCTTTGGCC CCATCAAGAG CATCGACATG 
TCCTGGGACT CCGTCACCAT GAAGCACAAG GGCTTTGCCT TCGTGGAGTA TGAGGTCCCC 
GAAGCTGCAC AGCTGGCCTT GGAGCAGATG AACTCGGTGA TGCTGGGGGG CAGGAACATC 
AAGGTGGGCA GACCCAGCAA CATAGGGCAG GCCCAGCCCA TCATAGACCA GTTGGCTGAG 
GAGGCACGGG CCTTCAACCG CATCTACGTG GCCTCTGTGC ACCAGGACCT CTCAGACGAT 
GACATCAAGA GCGTGTTTGA GGCCTTTGGC AAGATCAAGT CCTGCACACT GGCCCGGGAC 
CCCACAACTG GCAAGCACAA GGGCTACGGC TTCATTGAGT ACGAGAAGGC CCAGTCGTCC 
CAAGATGCTG TGTCTTCCAT GAACCTCTTT GACCTGGGTG GCCAGTACTT GCGGGTGGGC 
AAGGCTGTCA CACCGCCCAT GCCCCTACTC ACACCAGCCA CGCCTGGAGG CCTCCCACCT 
GCCGCTGCTG TGGCAGCTGC TGCAGCCACT GCCAAGATCA CAGCTCAGGA AGCAGTGGCC 
GGAGCAGCGG TGCTGGGTAC CCTGGGCACA CCTGGACTGG TGTCCCCAGC ACTGACCCTG 
GCCCAGCCCC TGGGCACTTT GCCCCAGGCT GTCATGGCTG CCCAGGCACC TGGAGTCATC 
ACAGGTGTGA CCCCAGCCCG TCCTCCTATC CCGGTCACCA TCCCCTCGGT GGGAGTGGTG 
AACCCCATCC TGGCCAGCCC TCCAACGCTG GGTCTCCTGG AGCCCAAGAA GGAGAAGGAA 
GAAGAGGAGC TGTTTCCCGA GTCAGAGCGG CCAGAGATGC TGAGCGAGCA GGAGCACATG 
AGCATCTCGG GCAGTAGCGC CCGACACATG GTGATGCAGA AGCTGCTCCG CAAGCAGGAG 
TCTACAGTGA TGGTTCTGCG CAACATGGTG GACCCCAAGG ACATCGATGA TGACCTGGAA 
GGGGAGGTGA CAGAGGAGTG TGGCAAGTTC GGGGCCGTGA ACCGCGTCAT CATCTACCAA 
GAGAAACAAG GCGAGGAGGA GGATGCAGAA ATCATTGTCA AGATCTTTGT GGAGTTTTCC 
ATAGCCTCTG AGACTCATAA GGCCATCCAG GCCCTCAATG GCCGCTGGTT TGCTGGCCGC 
AAGGTGGTGG CTGAAGTGTA CGACCAGGAG CGTTTTGATA ACAGTGACCT CTCTGCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_510965.1
CDS	84..1763
Translation	MATATIALQVNGQQGGGSEPAAAAAVVAAGDKWKPPQGTDSIKMENGQSTAAKLGLPPLTPEQQEALQKAKKYAMEQSIKSVLVKQTIAHQQQQLTNLQMAAVTMGFGDPLSPLQSMAAQRQRALAIMCRVYVGSIYYELGEDTIRQAFAPFGPIKSIDMSWDSVTMKHKGFAFVEYEVPEAAQLALEQMNSVMLGGRNIKVGRPSNIGQAQPIIDQLAEEARAFNRIYVASVHQDLSDDDIKSVFEAFGKIKSCTLARDPTTGKHKGYGFIEYEKAQSSQDAVSSMNLFDLGGQYLRVGKAVTPPMPLLTPATPGGLPPAAAVAAAAATAKITAQEAVAGAAVLGTLGTPGLVSPALTLAQPLGTLPQAVMAAQAPGVITGVTPARPPIPVTIPSVGVVNPILASPPTLGLLEPKKEKEEEELFPESERPEMLSEQEHMSISGSSARHMVMQKLLRKQESTVMVLRNMVDPKDIDDDLEGEVTEECGKFGAVNRVIIYQEKQGEEEDAEIIVKIFVEFSIASETHKAIQALNGRWFAGRKVVAEVYDQERFDNSDLSA

Target ORF information:

RefSeq Version	NM_078480.2
Organism	Homo sapiens(human)
Definition	Homo sapiens poly(U) binding splicing factor 60 (PUF60), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_078480.2

ORF Insert Sequence:

ATGGCGACGG CGACCATAGC TCTCCAGGTC AATGGCCAGC AAGGAGGGGG GTCCGAGCCG 
GCGGCGGCGG CGGCAGTGGT GGCAGCGGGA GACAAATGGA AACCTCCACA GGGCACAGAC 
TCCATCAAGA TGGAGAACGG GCAGAGCACA GCCGCCAAGC TGGGGCTGCC TCCCCTGACG 
CCCGAGCAGC AGGAGGCCCT TCAGAAGGCC AAGAAGTACG CCATGGAGCA GAGCATCAAG 
AGTGTGCTGG TGAAGCAGAC CATCGCGCAC CAGCAGCAGC AGCTCACCAA CCTGCAGATG 
GCAGCAGTGA CAATGGGCTT TGGAGATCCT CTCTCACCTT TGCAATCGAT GGCGGCTCAG 
CGGCAGCGGG CGCTGGCCAT CATGTGCCGC GTCTACGTGG GCTCTATCTA CTATGAGCTG 
GGGGAGGACA CCATCCGCCA GGCCTTTGCC CCCTTTGGCC CCATCAAGAG CATCGACATG 
TCCTGGGACT CCGTCACCAT GAAGCACAAG GGCTTTGCCT TCGTGGAGTA TGAGGTCCCC 
GAAGCTGCAC AGCTGGCCTT GGAGCAGATG AACTCGGTGA TGCTGGGGGG CAGGAACATC 
AAGGTGGGCA GACCCAGCAA CATAGGGCAG GCCCAGCCCA TCATAGACCA GTTGGCTGAG 
GAGGCACGGG CCTTCAACCG CATCTACGTG GCCTCTGTGC ACCAGGACCT CTCAGACGAT 
GACATCAAGA GCGTGTTTGA GGCCTTTGGC AAGATCAAGT CCTGCACACT GGCCCGGGAC 
CCCACAACTG GCAAGCACAA GGGCTACGGC TTCATTGAGT ACGAGAAGGC CCAGTCGTCC 
CAAGATGCTG TGTCTTCCAT GAACCTCTTT GACCTGGGTG GCCAGTACTT GCGGGTGGGC 
AAGGCTGTCA CACCGCCCAT GCCCCTACTC ACACCAGCCA CGCCTGGAGG CCTCCCACCT 
GCCGCTGCTG TGGCAGCTGC TGCAGCCACT GCCAAGATCA CAGCTCAGGA AGCAGTGGCC 
GGAGCAGCGG TGCTGGGTAC CCTGGGCACA CCTGGACTGG TGTCCCCAGC ACTGACCCTG 
GCCCAGCCCC TGGGCACTTT GCCCCAGGCT GTCATGGCTG CCCAGGCACC TGGAGTCATC 
ACAGGTGTGA CCCCAGCCCG TCCTCCTATC CCGGTCACCA TCCCCTCGGT GGGAGTGGTG 
AACCCCATCC TGGCCAGCCC TCCAACGCTG GGTCTCCTGG AGCCCAAGAA GGAGAAGGAA 
GAAGAGGAGC TGTTTCCCGA GTCAGAGCGG CCAGAGATGC TGAGCGAGCA GGAGCACATG 
AGCATCTCGG GCAGTAGCGC CCGACACATG GTGATGCAGA AGCTGCTCCG CAAGCAGGAG 
TCTACAGTGA TGGTTCTGCG CAACATGGTG GACCCCAAGG ACATCGATGA TGACCTGGAA 
GGGGAGGTGA CAGAGGAGTG TGGCAAGTTC GGGGCCGTGA ACCGCGTCAT CATCTACCAA 
GAGAAACAAG GCGAGGAGGA GGATGCAGAA ATCATTGTCA AGATCTTTGT GGAGTTTTCC 
ATAGCCTCTG AGACTCATAA GGCCATCCAG GCCCTCAATG GCCGCTGGTT TGCTGGCCGC 
AAGGTGGTGG CTGAAGTGTA CGACCAGGAG CGTTTTGATA ACAGTGACCT CTCTGCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PUF60 ( NM_078480.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_510965.1 Homo sapiens poly(U) binding splicing factor 60 (PUF60), transcript variant 1, mRNA.

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CloneID	OHu20314
Clone ID Related Accession (Same CDS sequence)	NM_078480.2 , NM_078480.3
Accession Version	NM_078480.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1680bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-24
Organism	Homo sapiens(human)
Product	poly(U)-binding-splicing factor PUF60 isoform a
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF114818.1, AF217197.2 and AW249619.1. This sequence is a reference standard in the RefSeqGene project. On May 17, 2019 this sequence version replaced NM_078480.2. Transcript Variant: This variant (1) encodes the isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.13392.1, SRR1803611.197650.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000526683.6/ ENSP00000434359.1 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCGACGG CGACCATAGC TCTCCAGGTC AATGGCCAGC AAGGAGGGGG GTCCGAGCCG 
GCGGCGGCGG CGGCAGTGGT GGCAGCGGGA GACAAATGGA AACCTCCACA GGGCACAGAC 
TCCATCAAGA TGGAGAACGG GCAGAGCACA GCCGCCAAGC TGGGGCTGCC TCCCCTGACG 
CCCGAGCAGC AGGAGGCCCT TCAGAAGGCC AAGAAGTACG CCATGGAGCA GAGCATCAAG 
AGTGTGCTGG TGAAGCAGAC CATCGCGCAC CAGCAGCAGC AGCTCACCAA CCTGCAGATG 
GCAGCAGTGA CAATGGGCTT TGGAGATCCT CTCTCACCTT TGCAATCGAT GGCGGCTCAG 
CGGCAGCGGG CGCTGGCCAT CATGTGCCGC GTCTACGTGG GCTCTATCTA CTATGAGCTG 
GGGGAGGACA CCATCCGCCA GGCCTTTGCC CCCTTTGGCC CCATCAAGAG CATCGACATG 
TCCTGGGACT CCGTCACCAT GAAGCACAAG GGCTTTGCCT TCGTGGAGTA TGAGGTCCCC 
GAAGCTGCAC AGCTGGCCTT GGAGCAGATG AACTCGGTGA TGCTGGGGGG CAGGAACATC 
AAGGTGGGCA GACCCAGCAA CATAGGGCAG GCCCAGCCCA TCATAGACCA GTTGGCTGAG 
GAGGCACGGG CCTTCAACCG CATCTACGTG GCCTCTGTGC ACCAGGACCT CTCAGACGAT 
GACATCAAGA GCGTGTTTGA GGCCTTTGGC AAGATCAAGT CCTGCACACT GGCCCGGGAC 
CCCACAACTG GCAAGCACAA GGGCTACGGC TTCATTGAGT ACGAGAAGGC CCAGTCGTCC 
CAAGATGCTG TGTCTTCCAT GAACCTCTTT GACCTGGGTG GCCAGTACTT GCGGGTGGGC 
AAGGCTGTCA CACCGCCCAT GCCCCTACTC ACACCAGCCA CGCCTGGAGG CCTCCCACCT 
GCCGCTGCTG TGGCAGCTGC TGCAGCCACT GCCAAGATCA CAGCTCAGGA AGCAGTGGCC 
GGAGCAGCGG TGCTGGGTAC CCTGGGCACA CCTGGACTGG TGTCCCCAGC ACTGACCCTG 
GCCCAGCCCC TGGGCACTTT GCCCCAGGCT GTCATGGCTG CCCAGGCACC TGGAGTCATC 
ACAGGTGTGA CCCCAGCCCG TCCTCCTATC CCGGTCACCA TCCCCTCGGT GGGAGTGGTG 
AACCCCATCC TGGCCAGCCC TCCAACGCTG GGTCTCCTGG AGCCCAAGAA GGAGAAGGAA 
GAAGAGGAGC TGTTTCCCGA GTCAGAGCGG CCAGAGATGC TGAGCGAGCA GGAGCACATG 
AGCATCTCGG GCAGTAGCGC CCGACACATG GTGATGCAGA AGCTGCTCCG CAAGCAGGAG 
TCTACAGTGA TGGTTCTGCG CAACATGGTG GACCCCAAGG ACATCGATGA TGACCTGGAA 
GGGGAGGTGA CAGAGGAGTG TGGCAAGTTC GGGGCCGTGA ACCGCGTCAT CATCTACCAA 
GAGAAACAAG GCGAGGAGGA GGATGCAGAA ATCATTGTCA AGATCTTTGT GGAGTTTTCC 
ATAGCCTCTG AGACTCATAA GGCCATCCAG GCCCTCAATG GCCGCTGGTT TGCTGGCCGC 
AAGGTGGTGG CTGAAGTGTA CGACCAGGAG CGTTTTGATA ACAGTGACCT CTCTGCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_510965.1
CDS	13..1692
Translation	MATATIALQVNGQQGGGSEPAAAAAVVAAGDKWKPPQGTDSIKMENGQSTAAKLGLPPLTPEQQEALQKAKKYAMEQSIKSVLVKQTIAHQQQQLTNLQMAAVTMGFGDPLSPLQSMAAQRQRALAIMCRVYVGSIYYELGEDTIRQAFAPFGPIKSIDMSWDSVTMKHKGFAFVEYEVPEAAQLALEQMNSVMLGGRNIKVGRPSNIGQAQPIIDQLAEEARAFNRIYVASVHQDLSDDDIKSVFEAFGKIKSCTLARDPTTGKHKGYGFIEYEKAQSSQDAVSSMNLFDLGGQYLRVGKAVTPPMPLLTPATPGGLPPAAAVAAAAATAKITAQEAVAGAAVLGTLGTPGLVSPALTLAQPLGTLPQAVMAAQAPGVITGVTPARPPIPVTIPSVGVVNPILASPPTLGLLEPKKEKEEEELFPESERPEMLSEQEHMSISGSSARHMVMQKLLRKQESTVMVLRNMVDPKDIDDDLEGEVTEECGKFGAVNRVIIYQEKQGEEEDAEIIVKIFVEFSIASETHKAIQALNGRWFAGRKVVAEVYDQERFDNSDLSA

Target ORF information:

RefSeq Version	NM_078480.3
Organism	Homo sapiens(human)
Definition	Homo sapiens poly(U) binding splicing factor 60 (PUF60), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_078480.3

ORF Insert Sequence:

ATGGCGACGG CGACCATAGC TCTCCAGGTC AATGGCCAGC AAGGAGGGGG GTCCGAGCCG 
GCGGCGGCGG CGGCAGTGGT GGCAGCGGGA GACAAATGGA AACCTCCACA GGGCACAGAC 
TCCATCAAGA TGGAGAACGG GCAGAGCACA GCCGCCAAGC TGGGGCTGCC TCCCCTGACG 
CCCGAGCAGC AGGAGGCCCT TCAGAAGGCC AAGAAGTACG CCATGGAGCA GAGCATCAAG 
AGTGTGCTGG TGAAGCAGAC CATCGCGCAC CAGCAGCAGC AGCTCACCAA CCTGCAGATG 
GCAGCAGTGA CAATGGGCTT TGGAGATCCT CTCTCACCTT TGCAATCGAT GGCGGCTCAG 
CGGCAGCGGG CGCTGGCCAT CATGTGCCGC GTCTACGTGG GCTCTATCTA CTATGAGCTG 
GGGGAGGACA CCATCCGCCA GGCCTTTGCC CCCTTTGGCC CCATCAAGAG CATCGACATG 
TCCTGGGACT CCGTCACCAT GAAGCACAAG GGCTTTGCCT TCGTGGAGTA TGAGGTCCCC 
GAAGCTGCAC AGCTGGCCTT GGAGCAGATG AACTCGGTGA TGCTGGGGGG CAGGAACATC 
AAGGTGGGCA GACCCAGCAA CATAGGGCAG GCCCAGCCCA TCATAGACCA GTTGGCTGAG 
GAGGCACGGG CCTTCAACCG CATCTACGTG GCCTCTGTGC ACCAGGACCT CTCAGACGAT 
GACATCAAGA GCGTGTTTGA GGCCTTTGGC AAGATCAAGT CCTGCACACT GGCCCGGGAC 
CCCACAACTG GCAAGCACAA GGGCTACGGC TTCATTGAGT ACGAGAAGGC CCAGTCGTCC 
CAAGATGCTG TGTCTTCCAT GAACCTCTTT GACCTGGGTG GCCAGTACTT GCGGGTGGGC 
AAGGCTGTCA CACCGCCCAT GCCCCTACTC ACACCAGCCA CGCCTGGAGG CCTCCCACCT 
GCCGCTGCTG TGGCAGCTGC TGCAGCCACT GCCAAGATCA CAGCTCAGGA AGCAGTGGCC 
GGAGCAGCGG TGCTGGGTAC CCTGGGCACA CCTGGACTGG TGTCCCCAGC ACTGACCCTG 
GCCCAGCCCC TGGGCACTTT GCCCCAGGCT GTCATGGCTG CCCAGGCACC TGGAGTCATC 
ACAGGTGTGA CCCCAGCCCG TCCTCCTATC CCGGTCACCA TCCCCTCGGT GGGAGTGGTG 
AACCCCATCC TGGCCAGCCC TCCAACGCTG GGTCTCCTGG AGCCCAAGAA GGAGAAGGAA 
GAAGAGGAGC TGTTTCCCGA GTCAGAGCGG CCAGAGATGC TGAGCGAGCA GGAGCACATG 
AGCATCTCGG GCAGTAGCGC CCGACACATG GTGATGCAGA AGCTGCTCCG CAAGCAGGAG 
TCTACAGTGA TGGTTCTGCG CAACATGGTG GACCCCAAGG ACATCGATGA TGACCTGGAA 
GGGGAGGTGA CAGAGGAGTG TGGCAAGTTC GGGGCCGTGA ACCGCGTCAT CATCTACCAA 
GAGAAACAAG GCGAGGAGGA GGATGCAGAA ATCATTGTCA AGATCTTTGT GGAGTTTTCC 
ATAGCCTCTG AGACTCATAA GGCCATCCAG GCCCTCAATG GCCGCTGGTT TGCTGGCCGC 
AAGGTGGTGG CTGAAGTGTA CGACCAGGAG CGTTTTGATA ACAGTGACCT CTCTGCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PUF60 ( NM_001271096.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001258025.1 Homo sapiens poly(U) binding splicing factor 60 (PUF60), transcript variant 4, mRNA.

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CloneID	OHu20744
Clone ID Related Accession (Same CDS sequence)	NM_001271096.2 , NM_001271096.1
Accession Version	NM_001271096.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1626bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-27
Organism	Homo sapiens(human)
Product	poly(U)-binding-splicing factor PUF60 isoform d
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BX418925.2, AC105219.6, AF217197.2 and AW249619.1. On May 31, 2019 this sequence version replaced NM_001271096.1. Transcript Variant: This variant (4) uses an alternate in-frame splice site and lacks an alternate exon in the coding region, compared to variant 1, but maintains the reading frame. The encoded isoform (d) is shorter than isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.273076.1, SRR1803616.186532.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGACGG CGACCATAGC TCTCGTCAAT GGCCAGCAAG GAGGGGGGTC CGAGCCGGCG 
GCGGCGGCGG CAGTGGTGGC AGCGGGAGAC AAATGGAAAC CTCCACAGGG CACAGACTCC 
ATCAAGATGG AGAACGGGCA GAGCACAGCC GCCAAGCTGG GGCTGCCTCC CCTGACGCCC 
GAGCAGCAGG AGGCCCTTCA GAAGGCCAAG AAGTACGCCA TGGAGCAGAG CATCAAGAGT 
GTGCTGGTGA AGCAGACCAT CGCGCACCAG CAGCAGCAGC TCACCAACCT GCAGATGGCG 
GCTCAGCGGC AGCGGGCGCT GGCCATCATG TGCCGCGTCT ACGTGGGCTC TATCTACTAT 
GAGCTGGGGG AGGACACCAT CCGCCAGGCC TTTGCCCCCT TTGGCCCCAT CAAGAGCATC 
GACATGTCCT GGGACTCCGT CACCATGAAG CACAAGGGCT TTGCCTTCGT GGAGTATGAG 
GTCCCCGAAG CTGCACAGCT GGCCTTGGAG CAGATGAACT CGGTGATGCT GGGGGGCAGG 
AACATCAAGG TGGGCAGACC CAGCAACATA GGGCAGGCCC AGCCCATCAT AGACCAGTTG 
GCTGAGGAGG CACGGGCCTT CAACCGCATC TACGTGGCCT CTGTGCACCA GGACCTCTCA 
GACGATGACA TCAAGAGCGT GTTTGAGGCC TTTGGCAAGA TCAAGTCCTG CACACTGGCC 
CGGGACCCCA CAACTGGCAA GCACAAGGGC TACGGCTTCA TTGAGTACGA GAAGGCCCAG 
TCGTCCCAAG ATGCTGTGTC TTCCATGAAC CTCTTTGACC TGGGTGGCCA GTACTTGCGG 
GTGGGCAAGG CTGTCACACC GCCCATGCCC CTACTCACAC CAGCCACGCC TGGAGGCCTC 
CCACCTGCCG CTGCTGTGGC AGCTGCTGCA GCCACTGCCA AGATCACAGC TCAGGAAGCA 
GTGGCCGGAG CAGCGGTGCT GGGTACCCTG GGCACACCTG GACTGGTGTC CCCAGCACTG 
ACCCTGGCCC AGCCCCTGGG CACTTTGCCC CAGGCTGTCA TGGCTGCCCA GGCACCTGGA 
GTCATCACAG GTGTGACCCC AGCCCGTCCT CCTATCCCGG TCACCATCCC CTCGGTGGGA 
GTGGTGAACC CCATCCTGGC CAGCCCTCCA ACGCTGGGTC TCCTGGAGCC CAAGAAGGAG 
AAGGAAGAAG AGGAGCTGTT TCCCGAGTCA GAGCGGCCAG AGATGCTGAG CGAGCAGGAG 
CACATGAGCA TCTCGGGCAG TAGCGCCCGA CACATGGTGA TGCAGAAGCT GCTCCGCAAG 
CAGGAGTCTA CAGTGATGGT TCTGCGCAAC ATGGTGGACC CCAAGGACAT CGATGATGAC 
CTGGAAGGGG AGGTGACAGA GGAGTGTGGC AAGTTCGGGG CCGTGAACCG CGTCATCATC 
TACCAAGAGA AACAAGGCGA GGAGGAGGAT GCAGAAATCA TTGTCAAGAT CTTTGTGGAG 
TTTTCCATAG CCTCTGAGAC TCATAAGGCC ATCCAGGCCC TCAATGGCCG CTGGTTTGCT 
GGCCGCAAGG TGGTGGCTGA AGTGTACGAC CAGGAGCGTT TTGATAACAG TGACCTCTCT 
GCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001258025.1
CDS	13..1638
Translation	MATATIALVNGQQGGGSEPAAAAAVVAAGDKWKPPQGTDSIKMENGQSTAAKLGLPPLTPEQQEALQKAKKYAMEQSIKSVLVKQTIAHQQQQLTNLQMAAQRQRALAIMCRVYVGSIYYELGEDTIRQAFAPFGPIKSIDMSWDSVTMKHKGFAFVEYEVPEAAQLALEQMNSVMLGGRNIKVGRPSNIGQAQPIIDQLAEEARAFNRIYVASVHQDLSDDDIKSVFEAFGKIKSCTLARDPTTGKHKGYGFIEYEKAQSSQDAVSSMNLFDLGGQYLRVGKAVTPPMPLLTPATPGGLPPAAAVAAAAATAKITAQEAVAGAAVLGTLGTPGLVSPALTLAQPLGTLPQAVMAAQAPGVITGVTPARPPIPVTIPSVGVVNPILASPPTLGLLEPKKEKEEEELFPESERPEMLSEQEHMSISGSSARHMVMQKLLRKQESTVMVLRNMVDPKDIDDDLEGEVTEECGKFGAVNRVIIYQEKQGEEEDAEIIVKIFVEFSIASETHKAIQALNGRWFAGRKVVAEVYDQERFDNSDLSA

Target ORF information:

RefSeq Version	NM_001271096.2
Organism	Homo sapiens(human)
Definition	Homo sapiens poly(U) binding splicing factor 60 (PUF60), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001271096.2

ORF Insert Sequence:

ATGGCGACGG CGACCATAGC TCTCGTCAAT GGCCAGCAAG GAGGGGGGTC CGAGCCGGCG 
GCGGCGGCGG CAGTGGTGGC AGCGGGAGAC AAATGGAAAC CTCCACAGGG CACAGACTCC 
ATCAAGATGG AGAACGGGCA GAGCACAGCC GCCAAGCTGG GGCTGCCTCC CCTGACGCCC 
GAGCAGCAGG AGGCCCTTCA GAAGGCCAAG AAGTACGCCA TGGAGCAGAG CATCAAGAGT 
GTGCTGGTGA AGCAGACCAT CGCGCACCAG CAGCAGCAGC TCACCAACCT GCAGATGGCG 
GCTCAGCGGC AGCGGGCGCT GGCCATCATG TGCCGCGTCT ACGTGGGCTC TATCTACTAT 
GAGCTGGGGG AGGACACCAT CCGCCAGGCC TTTGCCCCCT TTGGCCCCAT CAAGAGCATC 
GACATGTCCT GGGACTCCGT CACCATGAAG CACAAGGGCT TTGCCTTCGT GGAGTATGAG 
GTCCCCGAAG CTGCACAGCT GGCCTTGGAG CAGATGAACT CGGTGATGCT GGGGGGCAGG 
AACATCAAGG TGGGCAGACC CAGCAACATA GGGCAGGCCC AGCCCATCAT AGACCAGTTG 
GCTGAGGAGG CACGGGCCTT CAACCGCATC TACGTGGCCT CTGTGCACCA GGACCTCTCA 
GACGATGACA TCAAGAGCGT GTTTGAGGCC TTTGGCAAGA TCAAGTCCTG CACACTGGCC 
CGGGACCCCA CAACTGGCAA GCACAAGGGC TACGGCTTCA TTGAGTACGA GAAGGCCCAG 
TCGTCCCAAG ATGCTGTGTC TTCCATGAAC CTCTTTGACC TGGGTGGCCA GTACTTGCGG 
GTGGGCAAGG CTGTCACACC GCCCATGCCC CTACTCACAC CAGCCACGCC TGGAGGCCTC 
CCACCTGCCG CTGCTGTGGC AGCTGCTGCA GCCACTGCCA AGATCACAGC TCAGGAAGCA 
GTGGCCGGAG CAGCGGTGCT GGGTACCCTG GGCACACCTG GACTGGTGTC CCCAGCACTG 
ACCCTGGCCC AGCCCCTGGG CACTTTGCCC CAGGCTGTCA TGGCTGCCCA GGCACCTGGA 
GTCATCACAG GTGTGACCCC AGCCCGTCCT CCTATCCCGG TCACCATCCC CTCGGTGGGA 
GTGGTGAACC CCATCCTGGC CAGCCCTCCA ACGCTGGGTC TCCTGGAGCC CAAGAAGGAG 
AAGGAAGAAG AGGAGCTGTT TCCCGAGTCA GAGCGGCCAG AGATGCTGAG CGAGCAGGAG 
CACATGAGCA TCTCGGGCAG TAGCGCCCGA CACATGGTGA TGCAGAAGCT GCTCCGCAAG 
CAGGAGTCTA CAGTGATGGT TCTGCGCAAC ATGGTGGACC CCAAGGACAT CGATGATGAC 
CTGGAAGGGG AGGTGACAGA GGAGTGTGGC AAGTTCGGGG CCGTGAACCG CGTCATCATC 
TACCAAGAGA AACAAGGCGA GGAGGAGGAT GCAGAAATCA TTGTCAAGAT CTTTGTGGAG 
TTTTCCATAG CCTCTGAGAC TCATAAGGCC ATCCAGGCCC TCAATGGCCG CTGGTTTGCT 
GGCCGCAAGG TGGTGGCTGA AGTGTACGAC CAGGAGCGTT TTGATAACAG TGACCTCTCT 
GCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PUF60 ( NM_001271096.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001258025.1 Homo sapiens poly(U) binding splicing factor 60 (PUF60), transcript variant 4, mRNA.

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CloneID	OHu20744
Clone ID Related Accession (Same CDS sequence)	NM_001271096.2 , NM_001271096.1
Accession Version	NM_001271096.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1626bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-03
Organism	Homo sapiens(human)
Product	poly(U)-binding-splicing factor PUF60 isoform d
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CN371576.1, BX418925.2, AC105219.6, AF217197.2 and AW249619.1. Transcript Variant: This variant (4) uses an alternate in-frame splice site and lacks an alternate exon in the coding region, compared to variant 1, but maintains the reading frame. The encoded isoform (d) is shorter than isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR3476690.421056.1, SRR3476690.810305.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGACGG CGACCATAGC TCTCGTCAAT GGCCAGCAAG GAGGGGGGTC CGAGCCGGCG 
GCGGCGGCGG CAGTGGTGGC AGCGGGAGAC AAATGGAAAC CTCCACAGGG CACAGACTCC 
ATCAAGATGG AGAACGGGCA GAGCACAGCC GCCAAGCTGG GGCTGCCTCC CCTGACGCCC 
GAGCAGCAGG AGGCCCTTCA GAAGGCCAAG AAGTACGCCA TGGAGCAGAG CATCAAGAGT 
GTGCTGGTGA AGCAGACCAT CGCGCACCAG CAGCAGCAGC TCACCAACCT GCAGATGGCG 
GCTCAGCGGC AGCGGGCGCT GGCCATCATG TGCCGCGTCT ACGTGGGCTC TATCTACTAT 
GAGCTGGGGG AGGACACCAT CCGCCAGGCC TTTGCCCCCT TTGGCCCCAT CAAGAGCATC 
GACATGTCCT GGGACTCCGT CACCATGAAG CACAAGGGCT TTGCCTTCGT GGAGTATGAG 
GTCCCCGAAG CTGCACAGCT GGCCTTGGAG CAGATGAACT CGGTGATGCT GGGGGGCAGG 
AACATCAAGG TGGGCAGACC CAGCAACATA GGGCAGGCCC AGCCCATCAT AGACCAGTTG 
GCTGAGGAGG CACGGGCCTT CAACCGCATC TACGTGGCCT CTGTGCACCA GGACCTCTCA 
GACGATGACA TCAAGAGCGT GTTTGAGGCC TTTGGCAAGA TCAAGTCCTG CACACTGGCC 
CGGGACCCCA CAACTGGCAA GCACAAGGGC TACGGCTTCA TTGAGTACGA GAAGGCCCAG 
TCGTCCCAAG ATGCTGTGTC TTCCATGAAC CTCTTTGACC TGGGTGGCCA GTACTTGCGG 
GTGGGCAAGG CTGTCACACC GCCCATGCCC CTACTCACAC CAGCCACGCC TGGAGGCCTC 
CCACCTGCCG CTGCTGTGGC AGCTGCTGCA GCCACTGCCA AGATCACAGC TCAGGAAGCA 
GTGGCCGGAG CAGCGGTGCT GGGTACCCTG GGCACACCTG GACTGGTGTC CCCAGCACTG 
ACCCTGGCCC AGCCCCTGGG CACTTTGCCC CAGGCTGTCA TGGCTGCCCA GGCACCTGGA 
GTCATCACAG GTGTGACCCC AGCCCGTCCT CCTATCCCGG TCACCATCCC CTCGGTGGGA 
GTGGTGAACC CCATCCTGGC CAGCCCTCCA ACGCTGGGTC TCCTGGAGCC CAAGAAGGAG 
AAGGAAGAAG AGGAGCTGTT TCCCGAGTCA GAGCGGCCAG AGATGCTGAG CGAGCAGGAG 
CACATGAGCA TCTCGGGCAG TAGCGCCCGA CACATGGTGA TGCAGAAGCT GCTCCGCAAG 
CAGGAGTCTA CAGTGATGGT TCTGCGCAAC ATGGTGGACC CCAAGGACAT CGATGATGAC 
CTGGAAGGGG AGGTGACAGA GGAGTGTGGC AAGTTCGGGG CCGTGAACCG CGTCATCATC 
TACCAAGAGA AACAAGGCGA GGAGGAGGAT GCAGAAATCA TTGTCAAGAT CTTTGTGGAG 
TTTTCCATAG CCTCTGAGAC TCATAAGGCC ATCCAGGCCC TCAATGGCCG CTGGTTTGCT 
GGCCGCAAGG TGGTGGCTGA AGTGTACGAC CAGGAGCGTT TTGATAACAG TGACCTCTCT 
GCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001258025.1
CDS	84..1709
Translation	MATATIALVNGQQGGGSEPAAAAAVVAAGDKWKPPQGTDSIKMENGQSTAAKLGLPPLTPEQQEALQKAKKYAMEQSIKSVLVKQTIAHQQQQLTNLQMAAQRQRALAIMCRVYVGSIYYELGEDTIRQAFAPFGPIKSIDMSWDSVTMKHKGFAFVEYEVPEAAQLALEQMNSVMLGGRNIKVGRPSNIGQAQPIIDQLAEEARAFNRIYVASVHQDLSDDDIKSVFEAFGKIKSCTLARDPTTGKHKGYGFIEYEKAQSSQDAVSSMNLFDLGGQYLRVGKAVTPPMPLLTPATPGGLPPAAAVAAAAATAKITAQEAVAGAAVLGTLGTPGLVSPALTLAQPLGTLPQAVMAAQAPGVITGVTPARPPIPVTIPSVGVVNPILASPPTLGLLEPKKEKEEEELFPESERPEMLSEQEHMSISGSSARHMVMQKLLRKQESTVMVLRNMVDPKDIDDDLEGEVTEECGKFGAVNRVIIYQEKQGEEEDAEIIVKIFVEFSIASETHKAIQALNGRWFAGRKVVAEVYDQERFDNSDLSA

Target ORF information:

RefSeq Version	NM_001271096.1
Organism	Homo sapiens(human)
Definition	Homo sapiens poly(U) binding splicing factor 60 (PUF60), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001271096.1

ORF Insert Sequence:

ATGGCGACGG CGACCATAGC TCTCGTCAAT GGCCAGCAAG GAGGGGGGTC CGAGCCGGCG 
GCGGCGGCGG CAGTGGTGGC AGCGGGAGAC AAATGGAAAC CTCCACAGGG CACAGACTCC 
ATCAAGATGG AGAACGGGCA GAGCACAGCC GCCAAGCTGG GGCTGCCTCC CCTGACGCCC 
GAGCAGCAGG AGGCCCTTCA GAAGGCCAAG AAGTACGCCA TGGAGCAGAG CATCAAGAGT 
GTGCTGGTGA AGCAGACCAT CGCGCACCAG CAGCAGCAGC TCACCAACCT GCAGATGGCG 
GCTCAGCGGC AGCGGGCGCT GGCCATCATG TGCCGCGTCT ACGTGGGCTC TATCTACTAT 
GAGCTGGGGG AGGACACCAT CCGCCAGGCC TTTGCCCCCT TTGGCCCCAT CAAGAGCATC 
GACATGTCCT GGGACTCCGT CACCATGAAG CACAAGGGCT TTGCCTTCGT GGAGTATGAG 
GTCCCCGAAG CTGCACAGCT GGCCTTGGAG CAGATGAACT CGGTGATGCT GGGGGGCAGG 
AACATCAAGG TGGGCAGACC CAGCAACATA GGGCAGGCCC AGCCCATCAT AGACCAGTTG 
GCTGAGGAGG CACGGGCCTT CAACCGCATC TACGTGGCCT CTGTGCACCA GGACCTCTCA 
GACGATGACA TCAAGAGCGT GTTTGAGGCC TTTGGCAAGA TCAAGTCCTG CACACTGGCC 
CGGGACCCCA CAACTGGCAA GCACAAGGGC TACGGCTTCA TTGAGTACGA GAAGGCCCAG 
TCGTCCCAAG ATGCTGTGTC TTCCATGAAC CTCTTTGACC TGGGTGGCCA GTACTTGCGG 
GTGGGCAAGG CTGTCACACC GCCCATGCCC CTACTCACAC CAGCCACGCC TGGAGGCCTC 
CCACCTGCCG CTGCTGTGGC AGCTGCTGCA GCCACTGCCA AGATCACAGC TCAGGAAGCA 
GTGGCCGGAG CAGCGGTGCT GGGTACCCTG GGCACACCTG GACTGGTGTC CCCAGCACTG 
ACCCTGGCCC AGCCCCTGGG CACTTTGCCC CAGGCTGTCA TGGCTGCCCA GGCACCTGGA 
GTCATCACAG GTGTGACCCC AGCCCGTCCT CCTATCCCGG TCACCATCCC CTCGGTGGGA 
GTGGTGAACC CCATCCTGGC CAGCCCTCCA ACGCTGGGTC TCCTGGAGCC CAAGAAGGAG 
AAGGAAGAAG AGGAGCTGTT TCCCGAGTCA GAGCGGCCAG AGATGCTGAG CGAGCAGGAG 
CACATGAGCA TCTCGGGCAG TAGCGCCCGA CACATGGTGA TGCAGAAGCT GCTCCGCAAG 
CAGGAGTCTA CAGTGATGGT TCTGCGCAAC ATGGTGGACC CCAAGGACAT CGATGATGAC 
CTGGAAGGGG AGGTGACAGA GGAGTGTGGC AAGTTCGGGG CCGTGAACCG CGTCATCATC 
TACCAAGAGA AACAAGGCGA GGAGGAGGAT GCAGAAATCA TTGTCAAGAT CTTTGTGGAG 
TTTTCCATAG CCTCTGAGAC TCATAAGGCC ATCCAGGCCC TCAATGGCCG CTGGTTTGCT 
GGCCGCAAGG TGGTGGCTGA AGTGTACGAC CAGGAGCGTT TTGATAACAG TGACCTCTCT 
GCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PUF60 ( NM_001271098.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001258027.1 Homo sapiens poly(U) binding splicing factor 60 (PUF60), transcript variant 6, mRNA.

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CloneID	OHu21541
Clone ID Related Accession (Same CDS sequence)	NM_001271098.2 , NM_001271098.1
Accession Version	NM_001271098.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1677bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-27
Organism	Homo sapiens(human)
Product	poly(U)-binding-splicing factor PUF60 isoform f
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL522753.3, AC105219.6, AF217197.2 and AW249619.1. On May 31, 2019 this sequence version replaced NM_001271098.1. Transcript Variant: This variant (6) uses an alternate in-frame splice site in the coding region, compared to variant 1. The encoded isoform (f) is shorter than isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.15736.1, SRR1803615.184871.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGACGG CGACCATAGC TCTCGTCAAT GGCCAGCAAG GAGGGGGGTC CGAGCCGGCG 
GCGGCGGCGG CAGTGGTGGC AGCGGGAGAC AAATGGAAAC CTCCACAGGG CACAGACTCC 
ATCAAGATGG AGAACGGGCA GAGCACAGCC GCCAAGCTGG GGCTGCCTCC CCTGACGCCC 
GAGCAGCAGG AGGCCCTTCA GAAGGCCAAG AAGTACGCCA TGGAGCAGAG CATCAAGAGT 
GTGCTGGTGA AGCAGACCAT CGCGCACCAG CAGCAGCAGC TCACCAACCT GCAGATGGCA 
GCAGTGACAA TGGGCTTTGG AGATCCTCTC TCACCTTTGC AATCGATGGC GGCTCAGCGG 
CAGCGGGCGC TGGCCATCAT GTGCCGCGTC TACGTGGGCT CTATCTACTA TGAGCTGGGG 
GAGGACACCA TCCGCCAGGC CTTTGCCCCC TTTGGCCCCA TCAAGAGCAT CGACATGTCC 
TGGGACTCCG TCACCATGAA GCACAAGGGC TTTGCCTTCG TGGAGTATGA GGTCCCCGAA 
GCTGCACAGC TGGCCTTGGA GCAGATGAAC TCGGTGATGC TGGGGGGCAG GAACATCAAG 
GTGGGCAGAC CCAGCAACAT AGGGCAGGCC CAGCCCATCA TAGACCAGTT GGCTGAGGAG 
GCACGGGCCT TCAACCGCAT CTACGTGGCC TCTGTGCACC AGGACCTCTC AGACGATGAC 
ATCAAGAGCG TGTTTGAGGC CTTTGGCAAG ATCAAGTCCT GCACACTGGC CCGGGACCCC 
ACAACTGGCA AGCACAAGGG CTACGGCTTC ATTGAGTACG AGAAGGCCCA GTCGTCCCAA 
GATGCTGTGT CTTCCATGAA CCTCTTTGAC CTGGGTGGCC AGTACTTGCG GGTGGGCAAG 
GCTGTCACAC CGCCCATGCC CCTACTCACA CCAGCCACGC CTGGAGGCCT CCCACCTGCC 
GCTGCTGTGG CAGCTGCTGC AGCCACTGCC AAGATCACAG CTCAGGAAGC AGTGGCCGGA 
GCAGCGGTGC TGGGTACCCT GGGCACACCT GGACTGGTGT CCCCAGCACT GACCCTGGCC 
CAGCCCCTGG GCACTTTGCC CCAGGCTGTC ATGGCTGCCC AGGCACCTGG AGTCATCACA 
GGTGTGACCC CAGCCCGTCC TCCTATCCCG GTCACCATCC CCTCGGTGGG AGTGGTGAAC 
CCCATCCTGG CCAGCCCTCC AACGCTGGGT CTCCTGGAGC CCAAGAAGGA GAAGGAAGAA 
GAGGAGCTGT TTCCCGAGTC AGAGCGGCCA GAGATGCTGA GCGAGCAGGA GCACATGAGC 
ATCTCGGGCA GTAGCGCCCG ACACATGGTG ATGCAGAAGC TGCTCCGCAA GCAGGAGTCT 
ACAGTGATGG TTCTGCGCAA CATGGTGGAC CCCAAGGACA TCGATGATGA CCTGGAAGGG 
GAGGTGACAG AGGAGTGTGG CAAGTTCGGG GCCGTGAACC GCGTCATCAT CTACCAAGAG 
AAACAAGGCG AGGAGGAGGA TGCAGAAATC ATTGTCAAGA TCTTTGTGGA GTTTTCCATA 
GCCTCTGAGA CTCATAAGGC CATCCAGGCC CTCAATGGCC GCTGGTTTGC TGGCCGCAAG 
GTGGTGGCTG AAGTGTACGA CCAGGAGCGT TTTGATAACA GTGACCTCTC TGCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001258027.1
CDS	13..1689
Translation	MATATIALVNGQQGGGSEPAAAAAVVAAGDKWKPPQGTDSIKMENGQSTAAKLGLPPLTPEQQEALQKAKKYAMEQSIKSVLVKQTIAHQQQQLTNLQMAAVTMGFGDPLSPLQSMAAQRQRALAIMCRVYVGSIYYELGEDTIRQAFAPFGPIKSIDMSWDSVTMKHKGFAFVEYEVPEAAQLALEQMNSVMLGGRNIKVGRPSNIGQAQPIIDQLAEEARAFNRIYVASVHQDLSDDDIKSVFEAFGKIKSCTLARDPTTGKHKGYGFIEYEKAQSSQDAVSSMNLFDLGGQYLRVGKAVTPPMPLLTPATPGGLPPAAAVAAAAATAKITAQEAVAGAAVLGTLGTPGLVSPALTLAQPLGTLPQAVMAAQAPGVITGVTPARPPIPVTIPSVGVVNPILASPPTLGLLEPKKEKEEEELFPESERPEMLSEQEHMSISGSSARHMVMQKLLRKQESTVMVLRNMVDPKDIDDDLEGEVTEECGKFGAVNRVIIYQEKQGEEEDAEIIVKIFVEFSIASETHKAIQALNGRWFAGRKVVAEVYDQERFDNSDLSA

Target ORF information:

RefSeq Version	NM_001271098.2
Organism	Homo sapiens(human)
Definition	Homo sapiens poly(U) binding splicing factor 60 (PUF60), transcript variant 6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001271098.2

ORF Insert Sequence:

ATGGCGACGG CGACCATAGC TCTCGTCAAT GGCCAGCAAG GAGGGGGGTC CGAGCCGGCG 
GCGGCGGCGG CAGTGGTGGC AGCGGGAGAC AAATGGAAAC CTCCACAGGG CACAGACTCC 
ATCAAGATGG AGAACGGGCA GAGCACAGCC GCCAAGCTGG GGCTGCCTCC CCTGACGCCC 
GAGCAGCAGG AGGCCCTTCA GAAGGCCAAG AAGTACGCCA TGGAGCAGAG CATCAAGAGT 
GTGCTGGTGA AGCAGACCAT CGCGCACCAG CAGCAGCAGC TCACCAACCT GCAGATGGCA 
GCAGTGACAA TGGGCTTTGG AGATCCTCTC TCACCTTTGC AATCGATGGC GGCTCAGCGG 
CAGCGGGCGC TGGCCATCAT GTGCCGCGTC TACGTGGGCT CTATCTACTA TGAGCTGGGG 
GAGGACACCA TCCGCCAGGC CTTTGCCCCC TTTGGCCCCA TCAAGAGCAT CGACATGTCC 
TGGGACTCCG TCACCATGAA GCACAAGGGC TTTGCCTTCG TGGAGTATGA GGTCCCCGAA 
GCTGCACAGC TGGCCTTGGA GCAGATGAAC TCGGTGATGC TGGGGGGCAG GAACATCAAG 
GTGGGCAGAC CCAGCAACAT AGGGCAGGCC CAGCCCATCA TAGACCAGTT GGCTGAGGAG 
GCACGGGCCT TCAACCGCAT CTACGTGGCC TCTGTGCACC AGGACCTCTC AGACGATGAC 
ATCAAGAGCG TGTTTGAGGC CTTTGGCAAG ATCAAGTCCT GCACACTGGC CCGGGACCCC 
ACAACTGGCA AGCACAAGGG CTACGGCTTC ATTGAGTACG AGAAGGCCCA GTCGTCCCAA 
GATGCTGTGT CTTCCATGAA CCTCTTTGAC CTGGGTGGCC AGTACTTGCG GGTGGGCAAG 
GCTGTCACAC CGCCCATGCC CCTACTCACA CCAGCCACGC CTGGAGGCCT CCCACCTGCC 
GCTGCTGTGG CAGCTGCTGC AGCCACTGCC AAGATCACAG CTCAGGAAGC AGTGGCCGGA 
GCAGCGGTGC TGGGTACCCT GGGCACACCT GGACTGGTGT CCCCAGCACT GACCCTGGCC 
CAGCCCCTGG GCACTTTGCC CCAGGCTGTC ATGGCTGCCC AGGCACCTGG AGTCATCACA 
GGTGTGACCC CAGCCCGTCC TCCTATCCCG GTCACCATCC CCTCGGTGGG AGTGGTGAAC 
CCCATCCTGG CCAGCCCTCC AACGCTGGGT CTCCTGGAGC CCAAGAAGGA GAAGGAAGAA 
GAGGAGCTGT TTCCCGAGTC AGAGCGGCCA GAGATGCTGA GCGAGCAGGA GCACATGAGC 
ATCTCGGGCA GTAGCGCCCG ACACATGGTG ATGCAGAAGC TGCTCCGCAA GCAGGAGTCT 
ACAGTGATGG TTCTGCGCAA CATGGTGGAC CCCAAGGACA TCGATGATGA CCTGGAAGGG 
GAGGTGACAG AGGAGTGTGG CAAGTTCGGG GCCGTGAACC GCGTCATCAT CTACCAAGAG 
AAACAAGGCG AGGAGGAGGA TGCAGAAATC ATTGTCAAGA TCTTTGTGGA GTTTTCCATA 
GCCTCTGAGA CTCATAAGGC CATCCAGGCC CTCAATGGCC GCTGGTTTGC TGGCCGCAAG 
GTGGTGGCTG AAGTGTACGA CCAGGAGCGT TTTGATAACA GTGACCTCTC TGCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PUF60 ( NM_001271098.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001258027.1 Homo sapiens poly(U) binding splicing factor 60 (PUF60), transcript variant 6, mRNA.

Price & Availability↑

CloneID	OHu21541
Clone ID Related Accession (Same CDS sequence)	NM_001271098.2 , NM_001271098.1
Accession Version	NM_001271098.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1677bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-22
Organism	Homo sapiens(human)
Product	poly(U)-binding-splicing factor PUF60 isoform f
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CN371576.1, AL522753.3, AC105219.6, AF217197.2 and AW249619.1. Transcript Variant: This variant (6) uses an alternate in-frame splice site in the coding region, compared to variant 1. The encoded isoform (f) is shorter than isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR3476690.217690.1, SRR3476690.52274.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGACGG CGACCATAGC TCTCGTCAAT GGCCAGCAAG GAGGGGGGTC CGAGCCGGCG 
GCGGCGGCGG CAGTGGTGGC AGCGGGAGAC AAATGGAAAC CTCCACAGGG CACAGACTCC 
ATCAAGATGG AGAACGGGCA GAGCACAGCC GCCAAGCTGG GGCTGCCTCC CCTGACGCCC 
GAGCAGCAGG AGGCCCTTCA GAAGGCCAAG AAGTACGCCA TGGAGCAGAG CATCAAGAGT 
GTGCTGGTGA AGCAGACCAT CGCGCACCAG CAGCAGCAGC TCACCAACCT GCAGATGGCA 
GCAGTGACAA TGGGCTTTGG AGATCCTCTC TCACCTTTGC AATCGATGGC GGCTCAGCGG 
CAGCGGGCGC TGGCCATCAT GTGCCGCGTC TACGTGGGCT CTATCTACTA TGAGCTGGGG 
GAGGACACCA TCCGCCAGGC CTTTGCCCCC TTTGGCCCCA TCAAGAGCAT CGACATGTCC 
TGGGACTCCG TCACCATGAA GCACAAGGGC TTTGCCTTCG TGGAGTATGA GGTCCCCGAA 
GCTGCACAGC TGGCCTTGGA GCAGATGAAC TCGGTGATGC TGGGGGGCAG GAACATCAAG 
GTGGGCAGAC CCAGCAACAT AGGGCAGGCC CAGCCCATCA TAGACCAGTT GGCTGAGGAG 
GCACGGGCCT TCAACCGCAT CTACGTGGCC TCTGTGCACC AGGACCTCTC AGACGATGAC 
ATCAAGAGCG TGTTTGAGGC CTTTGGCAAG ATCAAGTCCT GCACACTGGC CCGGGACCCC 
ACAACTGGCA AGCACAAGGG CTACGGCTTC ATTGAGTACG AGAAGGCCCA GTCGTCCCAA 
GATGCTGTGT CTTCCATGAA CCTCTTTGAC CTGGGTGGCC AGTACTTGCG GGTGGGCAAG 
GCTGTCACAC CGCCCATGCC CCTACTCACA CCAGCCACGC CTGGAGGCCT CCCACCTGCC 
GCTGCTGTGG CAGCTGCTGC AGCCACTGCC AAGATCACAG CTCAGGAAGC AGTGGCCGGA 
GCAGCGGTGC TGGGTACCCT GGGCACACCT GGACTGGTGT CCCCAGCACT GACCCTGGCC 
CAGCCCCTGG GCACTTTGCC CCAGGCTGTC ATGGCTGCCC AGGCACCTGG AGTCATCACA 
GGTGTGACCC CAGCCCGTCC TCCTATCCCG GTCACCATCC CCTCGGTGGG AGTGGTGAAC 
CCCATCCTGG CCAGCCCTCC AACGCTGGGT CTCCTGGAGC CCAAGAAGGA GAAGGAAGAA 
GAGGAGCTGT TTCCCGAGTC AGAGCGGCCA GAGATGCTGA GCGAGCAGGA GCACATGAGC 
ATCTCGGGCA GTAGCGCCCG ACACATGGTG ATGCAGAAGC TGCTCCGCAA GCAGGAGTCT 
ACAGTGATGG TTCTGCGCAA CATGGTGGAC CCCAAGGACA TCGATGATGA CCTGGAAGGG 
GAGGTGACAG AGGAGTGTGG CAAGTTCGGG GCCGTGAACC GCGTCATCAT CTACCAAGAG 
AAACAAGGCG AGGAGGAGGA TGCAGAAATC ATTGTCAAGA TCTTTGTGGA GTTTTCCATA 
GCCTCTGAGA CTCATAAGGC CATCCAGGCC CTCAATGGCC GCTGGTTTGC TGGCCGCAAG 
GTGGTGGCTG AAGTGTACGA CCAGGAGCGT TTTGATAACA GTGACCTCTC TGCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001258027.1
CDS	84..1760
Translation	MATATIALVNGQQGGGSEPAAAAAVVAAGDKWKPPQGTDSIKMENGQSTAAKLGLPPLTPEQQEALQKAKKYAMEQSIKSVLVKQTIAHQQQQLTNLQMAAVTMGFGDPLSPLQSMAAQRQRALAIMCRVYVGSIYYELGEDTIRQAFAPFGPIKSIDMSWDSVTMKHKGFAFVEYEVPEAAQLALEQMNSVMLGGRNIKVGRPSNIGQAQPIIDQLAEEARAFNRIYVASVHQDLSDDDIKSVFEAFGKIKSCTLARDPTTGKHKGYGFIEYEKAQSSQDAVSSMNLFDLGGQYLRVGKAVTPPMPLLTPATPGGLPPAAAVAAAAATAKITAQEAVAGAAVLGTLGTPGLVSPALTLAQPLGTLPQAVMAAQAPGVITGVTPARPPIPVTIPSVGVVNPILASPPTLGLLEPKKEKEEEELFPESERPEMLSEQEHMSISGSSARHMVMQKLLRKQESTVMVLRNMVDPKDIDDDLEGEVTEECGKFGAVNRVIIYQEKQGEEEDAEIIVKIFVEFSIASETHKAIQALNGRWFAGRKVVAEVYDQERFDNSDLSA

Target ORF information:

RefSeq Version	NM_001271098.1
Organism	Homo sapiens(human)
Definition	Homo sapiens poly(U) binding splicing factor 60 (PUF60), transcript variant 6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001271098.1

ORF Insert Sequence:

ATGGCGACGG CGACCATAGC TCTCGTCAAT GGCCAGCAAG GAGGGGGGTC CGAGCCGGCG 
GCGGCGGCGG CAGTGGTGGC AGCGGGAGAC AAATGGAAAC CTCCACAGGG CACAGACTCC 
ATCAAGATGG AGAACGGGCA GAGCACAGCC GCCAAGCTGG GGCTGCCTCC CCTGACGCCC 
GAGCAGCAGG AGGCCCTTCA GAAGGCCAAG AAGTACGCCA TGGAGCAGAG CATCAAGAGT 
GTGCTGGTGA AGCAGACCAT CGCGCACCAG CAGCAGCAGC TCACCAACCT GCAGATGGCA 
GCAGTGACAA TGGGCTTTGG AGATCCTCTC TCACCTTTGC AATCGATGGC GGCTCAGCGG 
CAGCGGGCGC TGGCCATCAT GTGCCGCGTC TACGTGGGCT CTATCTACTA TGAGCTGGGG 
GAGGACACCA TCCGCCAGGC CTTTGCCCCC TTTGGCCCCA TCAAGAGCAT CGACATGTCC 
TGGGACTCCG TCACCATGAA GCACAAGGGC TTTGCCTTCG TGGAGTATGA GGTCCCCGAA 
GCTGCACAGC TGGCCTTGGA GCAGATGAAC TCGGTGATGC TGGGGGGCAG GAACATCAAG 
GTGGGCAGAC CCAGCAACAT AGGGCAGGCC CAGCCCATCA TAGACCAGTT GGCTGAGGAG 
GCACGGGCCT TCAACCGCAT CTACGTGGCC TCTGTGCACC AGGACCTCTC AGACGATGAC 
ATCAAGAGCG TGTTTGAGGC CTTTGGCAAG ATCAAGTCCT GCACACTGGC CCGGGACCCC 
ACAACTGGCA AGCACAAGGG CTACGGCTTC ATTGAGTACG AGAAGGCCCA GTCGTCCCAA 
GATGCTGTGT CTTCCATGAA CCTCTTTGAC CTGGGTGGCC AGTACTTGCG GGTGGGCAAG 
GCTGTCACAC CGCCCATGCC CCTACTCACA CCAGCCACGC CTGGAGGCCT CCCACCTGCC 
GCTGCTGTGG CAGCTGCTGC AGCCACTGCC AAGATCACAG CTCAGGAAGC AGTGGCCGGA 
GCAGCGGTGC TGGGTACCCT GGGCACACCT GGACTGGTGT CCCCAGCACT GACCCTGGCC 
CAGCCCCTGG GCACTTTGCC CCAGGCTGTC ATGGCTGCCC AGGCACCTGG AGTCATCACA 
GGTGTGACCC CAGCCCGTCC TCCTATCCCG GTCACCATCC CCTCGGTGGG AGTGGTGAAC 
CCCATCCTGG CCAGCCCTCC AACGCTGGGT CTCCTGGAGC CCAAGAAGGA GAAGGAAGAA 
GAGGAGCTGT TTCCCGAGTC AGAGCGGCCA GAGATGCTGA GCGAGCAGGA GCACATGAGC 
ATCTCGGGCA GTAGCGCCCG ACACATGGTG ATGCAGAAGC TGCTCCGCAA GCAGGAGTCT 
ACAGTGATGG TTCTGCGCAA CATGGTGGAC CCCAAGGACA TCGATGATGA CCTGGAAGGG 
GAGGTGACAG AGGAGTGTGG CAAGTTCGGG GCCGTGAACC GCGTCATCAT CTACCAAGAG 
AAACAAGGCG AGGAGGAGGA TGCAGAAATC ATTGTCAAGA TCTTTGTGGA GTTTTCCATA 
GCCTCTGAGA CTCATAAGGC CATCCAGGCC CTCAATGGCC GCTGGTTTGC TGGCCGCAAG 
GTGGTGGCTG AAGTGTACGA CCAGGAGCGT TTTGATAACA GTGACCTCTC TGCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PUF60 ( NM_001271099.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001258028.1 Homo sapiens poly(U) binding splicing factor 60 (PUF60), transcript variant 7, mRNA.

Price & Availability↑

CloneID	OHu27468
Clone ID Related Accession (Same CDS sequence)	NM_001271099.2 , NM_001271099.1
Accession Version	NM_001271099.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1593bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-27
Organism	Homo sapiens(human)
Product	poly(U)-binding-splicing factor PUF60 isoform g
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CT000277.1, AF217197.2 and AW249619.1. On May 31, 2019 this sequence version replaced NM_001271099.1. Transcript Variant: This variant (7, also known as PUF60deltaexon2) lacks an alternate in-frame exon in the coding region, compared to variant 1. The encoded isoform (g) is shorter than isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.17120.1, SRR1660809.127209.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGACGG CGACCATAGC TCTCGGCACA GACTCCATCA AGATGGAGAA CGGGCAGAGC 
ACAGCCGCCA AGCTGGGGCT GCCTCCCCTG ACGCCCGAGC AGCAGGAGGC CCTTCAGAAG 
GCCAAGAAGT ACGCCATGGA GCAGAGCATC AAGAGTGTGC TGGTGAAGCA GACCATCGCG 
CACCAGCAGC AGCAGCTCAC CAACCTGCAG ATGGCAGCAG TGACAATGGG CTTTGGAGAT 
CCTCTCTCAC CTTTGCAATC GATGGCGGCT CAGCGGCAGC GGGCGCTGGC CATCATGTGC 
CGCGTCTACG TGGGCTCTAT CTACTATGAG CTGGGGGAGG ACACCATCCG CCAGGCCTTT 
GCCCCCTTTG GCCCCATCAA GAGCATCGAC ATGTCCTGGG ACTCCGTCAC CATGAAGCAC 
AAGGGCTTTG CCTTCGTGGA GTATGAGGTC CCCGAAGCTG CACAGCTGGC CTTGGAGCAG 
ATGAACTCGG TGATGCTGGG GGGCAGGAAC ATCAAGGTGG GCAGACCCAG CAACATAGGG 
CAGGCCCAGC CCATCATAGA CCAGTTGGCT GAGGAGGCAC GGGCCTTCAA CCGCATCTAC 
GTGGCCTCTG TGCACCAGGA CCTCTCAGAC GATGACATCA AGAGCGTGTT TGAGGCCTTT 
GGCAAGATCA AGTCCTGCAC ACTGGCCCGG GACCCCACAA CTGGCAAGCA CAAGGGCTAC 
GGCTTCATTG AGTACGAGAA GGCCCAGTCG TCCCAAGATG CTGTGTCTTC CATGAACCTC 
TTTGACCTGG GTGGCCAGTA CTTGCGGGTG GGCAAGGCTG TCACACCGCC CATGCCCCTA 
CTCACACCAG CCACGCCTGG AGGCCTCCCA CCTGCCGCTG CTGTGGCAGC TGCTGCAGCC 
ACTGCCAAGA TCACAGCTCA GGAAGCAGTG GCCGGAGCAG CGGTGCTGGG TACCCTGGGC 
ACACCTGGAC TGGTGTCCCC AGCACTGACC CTGGCCCAGC CCCTGGGCAC TTTGCCCCAG 
GCTGTCATGG CTGCCCAGGC ACCTGGAGTC ATCACAGGTG TGACCCCAGC CCGTCCTCCT 
ATCCCGGTCA CCATCCCCTC GGTGGGAGTG GTGAACCCCA TCCTGGCCAG CCCTCCAACG 
CTGGGTCTCC TGGAGCCCAA GAAGGAGAAG GAAGAAGAGG AGCTGTTTCC CGAGTCAGAG 
CGGCCAGAGA TGCTGAGCGA GCAGGAGCAC ATGAGCATCT CGGGCAGTAG CGCCCGACAC 
ATGGTGATGC AGAAGCTGCT CCGCAAGCAG GAGTCTACAG TGATGGTTCT GCGCAACATG 
GTGGACCCCA AGGACATCGA TGATGACCTG GAAGGGGAGG TGACAGAGGA GTGTGGCAAG 
TTCGGGGCCG TGAACCGCGT CATCATCTAC CAAGAGAAAC AAGGCGAGGA GGAGGATGCA 
GAAATCATTG TCAAGATCTT TGTGGAGTTT TCCATAGCCT CTGAGACTCA TAAGGCCATC 
CAGGCCCTCA ATGGCCGCTG GTTTGCTGGC CGCAAGGTGG TGGCTGAAGT GTACGACCAG 
GAGCGTTTTG ATAACAGTGA CCTCTCTGCG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001258028.1
CDS	13..1605
Translation	MATATIALGTDSIKMENGQSTAAKLGLPPLTPEQQEALQKAKKYAMEQSIKSVLVKQTIAHQQQQLTNLQMAAVTMGFGDPLSPLQSMAAQRQRALAIMCRVYVGSIYYELGEDTIRQAFAPFGPIKSIDMSWDSVTMKHKGFAFVEYEVPEAAQLALEQMNSVMLGGRNIKVGRPSNIGQAQPIIDQLAEEARAFNRIYVASVHQDLSDDDIKSVFEAFGKIKSCTLARDPTTGKHKGYGFIEYEKAQSSQDAVSSMNLFDLGGQYLRVGKAVTPPMPLLTPATPGGLPPAAAVAAAAATAKITAQEAVAGAAVLGTLGTPGLVSPALTLAQPLGTLPQAVMAAQAPGVITGVTPARPPIPVTIPSVGVVNPILASPPTLGLLEPKKEKEEEELFPESERPEMLSEQEHMSISGSSARHMVMQKLLRKQESTVMVLRNMVDPKDIDDDLEGEVTEECGKFGAVNRVIIYQEKQGEEEDAEIIVKIFVEFSIASETHKAIQALNGRWFAGRKVVAEVYDQERFDNSDLSA

Target ORF information:

RefSeq Version	NM_001271099.2
Organism	Homo sapiens(human)
Definition	Homo sapiens poly(U) binding splicing factor 60 (PUF60), transcript variant 7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001271099.2

ORF Insert Sequence:

ATGGCGACGG CGACCATAGC TCTCGGCACA GACTCCATCA AGATGGAGAA CGGGCAGAGC 
ACAGCCGCCA AGCTGGGGCT GCCTCCCCTG ACGCCCGAGC AGCAGGAGGC CCTTCAGAAG 
GCCAAGAAGT ACGCCATGGA GCAGAGCATC AAGAGTGTGC TGGTGAAGCA GACCATCGCG 
CACCAGCAGC AGCAGCTCAC CAACCTGCAG ATGGCAGCAG TGACAATGGG CTTTGGAGAT 
CCTCTCTCAC CTTTGCAATC GATGGCGGCT CAGCGGCAGC GGGCGCTGGC CATCATGTGC 
CGCGTCTACG TGGGCTCTAT CTACTATGAG CTGGGGGAGG ACACCATCCG CCAGGCCTTT 
GCCCCCTTTG GCCCCATCAA GAGCATCGAC ATGTCCTGGG ACTCCGTCAC CATGAAGCAC 
AAGGGCTTTG CCTTCGTGGA GTATGAGGTC CCCGAAGCTG CACAGCTGGC CTTGGAGCAG 
ATGAACTCGG TGATGCTGGG GGGCAGGAAC ATCAAGGTGG GCAGACCCAG CAACATAGGG 
CAGGCCCAGC CCATCATAGA CCAGTTGGCT GAGGAGGCAC GGGCCTTCAA CCGCATCTAC 
GTGGCCTCTG TGCACCAGGA CCTCTCAGAC GATGACATCA AGAGCGTGTT TGAGGCCTTT 
GGCAAGATCA AGTCCTGCAC ACTGGCCCGG GACCCCACAA CTGGCAAGCA CAAGGGCTAC 
GGCTTCATTG AGTACGAGAA GGCCCAGTCG TCCCAAGATG CTGTGTCTTC CATGAACCTC 
TTTGACCTGG GTGGCCAGTA CTTGCGGGTG GGCAAGGCTG TCACACCGCC CATGCCCCTA 
CTCACACCAG CCACGCCTGG AGGCCTCCCA CCTGCCGCTG CTGTGGCAGC TGCTGCAGCC 
ACTGCCAAGA TCACAGCTCA GGAAGCAGTG GCCGGAGCAG CGGTGCTGGG TACCCTGGGC 
ACACCTGGAC TGGTGTCCCC AGCACTGACC CTGGCCCAGC CCCTGGGCAC TTTGCCCCAG 
GCTGTCATGG CTGCCCAGGC ACCTGGAGTC ATCACAGGTG TGACCCCAGC CCGTCCTCCT 
ATCCCGGTCA CCATCCCCTC GGTGGGAGTG GTGAACCCCA TCCTGGCCAG CCCTCCAACG 
CTGGGTCTCC TGGAGCCCAA GAAGGAGAAG GAAGAAGAGG AGCTGTTTCC CGAGTCAGAG 
CGGCCAGAGA TGCTGAGCGA GCAGGAGCAC ATGAGCATCT CGGGCAGTAG CGCCCGACAC 
ATGGTGATGC AGAAGCTGCT CCGCAAGCAG GAGTCTACAG TGATGGTTCT GCGCAACATG 
GTGGACCCCA AGGACATCGA TGATGACCTG GAAGGGGAGG TGACAGAGGA GTGTGGCAAG 
TTCGGGGCCG TGAACCGCGT CATCATCTAC CAAGAGAAAC AAGGCGAGGA GGAGGATGCA 
GAAATCATTG TCAAGATCTT TGTGGAGTTT TCCATAGCCT CTGAGACTCA TAAGGCCATC 
CAGGCCCTCA ATGGCCGCTG GTTTGCTGGC CGCAAGGTGG TGGCTGAAGT GTACGACCAG 
GAGCGTTTTG ATAACAGTGA CCTCTCTGCG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PUF60 ( NM_001271099.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001258028.1 Homo sapiens poly(U) binding splicing factor 60 (PUF60), transcript variant 7, mRNA.

Price & Availability↑

CloneID	OHu27468
Clone ID Related Accession (Same CDS sequence)	NM_001271099.2 , NM_001271099.1
Accession Version	NM_001271099.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1593bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-03
Organism	Homo sapiens(human)
Product	poly(U)-binding-splicing factor PUF60 isoform g
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CN371576.1, CT000277.1, AF217197.2 and AW249619.1. Transcript Variant: This variant (7, also known as PUF60deltaexon2) lacks an alternate in-frame exon in the coding region, compared to variant 1. The encoded isoform (g) is shorter than isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR3476690.187996.1, SRR3476690.1147880.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGACGG CGACCATAGC TCTCGGCACA GACTCCATCA AGATGGAGAA CGGGCAGAGC 
ACAGCCGCCA AGCTGGGGCT GCCTCCCCTG ACGCCCGAGC AGCAGGAGGC CCTTCAGAAG 
GCCAAGAAGT ACGCCATGGA GCAGAGCATC AAGAGTGTGC TGGTGAAGCA GACCATCGCG 
CACCAGCAGC AGCAGCTCAC CAACCTGCAG ATGGCAGCAG TGACAATGGG CTTTGGAGAT 
CCTCTCTCAC CTTTGCAATC GATGGCGGCT CAGCGGCAGC GGGCGCTGGC CATCATGTGC 
CGCGTCTACG TGGGCTCTAT CTACTATGAG CTGGGGGAGG ACACCATCCG CCAGGCCTTT 
GCCCCCTTTG GCCCCATCAA GAGCATCGAC ATGTCCTGGG ACTCCGTCAC CATGAAGCAC 
AAGGGCTTTG CCTTCGTGGA GTATGAGGTC CCCGAAGCTG CACAGCTGGC CTTGGAGCAG 
ATGAACTCGG TGATGCTGGG GGGCAGGAAC ATCAAGGTGG GCAGACCCAG CAACATAGGG 
CAGGCCCAGC CCATCATAGA CCAGTTGGCT GAGGAGGCAC GGGCCTTCAA CCGCATCTAC 
GTGGCCTCTG TGCACCAGGA CCTCTCAGAC GATGACATCA AGAGCGTGTT TGAGGCCTTT 
GGCAAGATCA AGTCCTGCAC ACTGGCCCGG GACCCCACAA CTGGCAAGCA CAAGGGCTAC 
GGCTTCATTG AGTACGAGAA GGCCCAGTCG TCCCAAGATG CTGTGTCTTC CATGAACCTC 
TTTGACCTGG GTGGCCAGTA CTTGCGGGTG GGCAAGGCTG TCACACCGCC CATGCCCCTA 
CTCACACCAG CCACGCCTGG AGGCCTCCCA CCTGCCGCTG CTGTGGCAGC TGCTGCAGCC 
ACTGCCAAGA TCACAGCTCA GGAAGCAGTG GCCGGAGCAG CGGTGCTGGG TACCCTGGGC 
ACACCTGGAC TGGTGTCCCC AGCACTGACC CTGGCCCAGC CCCTGGGCAC TTTGCCCCAG 
GCTGTCATGG CTGCCCAGGC ACCTGGAGTC ATCACAGGTG TGACCCCAGC CCGTCCTCCT 
ATCCCGGTCA CCATCCCCTC GGTGGGAGTG GTGAACCCCA TCCTGGCCAG CCCTCCAACG 
CTGGGTCTCC TGGAGCCCAA GAAGGAGAAG GAAGAAGAGG AGCTGTTTCC CGAGTCAGAG 
CGGCCAGAGA TGCTGAGCGA GCAGGAGCAC ATGAGCATCT CGGGCAGTAG CGCCCGACAC 
ATGGTGATGC AGAAGCTGCT CCGCAAGCAG GAGTCTACAG TGATGGTTCT GCGCAACATG 
GTGGACCCCA AGGACATCGA TGATGACCTG GAAGGGGAGG TGACAGAGGA GTGTGGCAAG 
TTCGGGGCCG TGAACCGCGT CATCATCTAC CAAGAGAAAC AAGGCGAGGA GGAGGATGCA 
GAAATCATTG TCAAGATCTT TGTGGAGTTT TCCATAGCCT CTGAGACTCA TAAGGCCATC 
CAGGCCCTCA ATGGCCGCTG GTTTGCTGGC CGCAAGGTGG TGGCTGAAGT GTACGACCAG 
GAGCGTTTTG ATAACAGTGA CCTCTCTGCG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001258028.1
CDS	84..1676
Translation	MATATIALGTDSIKMENGQSTAAKLGLPPLTPEQQEALQKAKKYAMEQSIKSVLVKQTIAHQQQQLTNLQMAAVTMGFGDPLSPLQSMAAQRQRALAIMCRVYVGSIYYELGEDTIRQAFAPFGPIKSIDMSWDSVTMKHKGFAFVEYEVPEAAQLALEQMNSVMLGGRNIKVGRPSNIGQAQPIIDQLAEEARAFNRIYVASVHQDLSDDDIKSVFEAFGKIKSCTLARDPTTGKHKGYGFIEYEKAQSSQDAVSSMNLFDLGGQYLRVGKAVTPPMPLLTPATPGGLPPAAAVAAAAATAKITAQEAVAGAAVLGTLGTPGLVSPALTLAQPLGTLPQAVMAAQAPGVITGVTPARPPIPVTIPSVGVVNPILASPPTLGLLEPKKEKEEEELFPESERPEMLSEQEHMSISGSSARHMVMQKLLRKQESTVMVLRNMVDPKDIDDDLEGEVTEECGKFGAVNRVIIYQEKQGEEEDAEIIVKIFVEFSIASETHKAIQALNGRWFAGRKVVAEVYDQERFDNSDLSA

Target ORF information:

RefSeq Version	NM_001271099.1
Organism	Homo sapiens(human)
Definition	Homo sapiens poly(U) binding splicing factor 60 (PUF60), transcript variant 7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001271099.1

ORF Insert Sequence:

ATGGCGACGG CGACCATAGC TCTCGGCACA GACTCCATCA AGATGGAGAA CGGGCAGAGC 
ACAGCCGCCA AGCTGGGGCT GCCTCCCCTG ACGCCCGAGC AGCAGGAGGC CCTTCAGAAG 
GCCAAGAAGT ACGCCATGGA GCAGAGCATC AAGAGTGTGC TGGTGAAGCA GACCATCGCG 
CACCAGCAGC AGCAGCTCAC CAACCTGCAG ATGGCAGCAG TGACAATGGG CTTTGGAGAT 
CCTCTCTCAC CTTTGCAATC GATGGCGGCT CAGCGGCAGC GGGCGCTGGC CATCATGTGC 
CGCGTCTACG TGGGCTCTAT CTACTATGAG CTGGGGGAGG ACACCATCCG CCAGGCCTTT 
GCCCCCTTTG GCCCCATCAA GAGCATCGAC ATGTCCTGGG ACTCCGTCAC CATGAAGCAC 
AAGGGCTTTG CCTTCGTGGA GTATGAGGTC CCCGAAGCTG CACAGCTGGC CTTGGAGCAG 
ATGAACTCGG TGATGCTGGG GGGCAGGAAC ATCAAGGTGG GCAGACCCAG CAACATAGGG 
CAGGCCCAGC CCATCATAGA CCAGTTGGCT GAGGAGGCAC GGGCCTTCAA CCGCATCTAC 
GTGGCCTCTG TGCACCAGGA CCTCTCAGAC GATGACATCA AGAGCGTGTT TGAGGCCTTT 
GGCAAGATCA AGTCCTGCAC ACTGGCCCGG GACCCCACAA CTGGCAAGCA CAAGGGCTAC 
GGCTTCATTG AGTACGAGAA GGCCCAGTCG TCCCAAGATG CTGTGTCTTC CATGAACCTC 
TTTGACCTGG GTGGCCAGTA CTTGCGGGTG GGCAAGGCTG TCACACCGCC CATGCCCCTA 
CTCACACCAG CCACGCCTGG AGGCCTCCCA CCTGCCGCTG CTGTGGCAGC TGCTGCAGCC 
ACTGCCAAGA TCACAGCTCA GGAAGCAGTG GCCGGAGCAG CGGTGCTGGG TACCCTGGGC 
ACACCTGGAC TGGTGTCCCC AGCACTGACC CTGGCCCAGC CCCTGGGCAC TTTGCCCCAG 
GCTGTCATGG CTGCCCAGGC ACCTGGAGTC ATCACAGGTG TGACCCCAGC CCGTCCTCCT 
ATCCCGGTCA CCATCCCCTC GGTGGGAGTG GTGAACCCCA TCCTGGCCAG CCCTCCAACG 
CTGGGTCTCC TGGAGCCCAA GAAGGAGAAG GAAGAAGAGG AGCTGTTTCC CGAGTCAGAG 
CGGCCAGAGA TGCTGAGCGA GCAGGAGCAC ATGAGCATCT CGGGCAGTAG CGCCCGACAC 
ATGGTGATGC AGAAGCTGCT CCGCAAGCAG GAGTCTACAG TGATGGTTCT GCGCAACATG 
GTGGACCCCA AGGACATCGA TGATGACCTG GAAGGGGAGG TGACAGAGGA GTGTGGCAAG 
TTCGGGGCCG TGAACCGCGT CATCATCTAC CAAGAGAAAC AAGGCGAGGA GGAGGATGCA 
GAAATCATTG TCAAGATCTT TGTGGAGTTT TCCATAGCCT CTGAGACTCA TAAGGCCATC 
CAGGCCCTCA ATGGCCGCTG GTTTGCTGGC CGCAAGGTGG TGGCTGAAGT GTACGACCAG 
GAGCGTTTTG ATAACAGTGA CCTCTCTGCG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PUF60 ( NM_001271097.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001258026.1 Homo sapiens poly(U) binding splicing factor 60 (PUF60), transcript variant 5, mRNA.

Price & Availability↑

CloneID	OHu27624
Clone ID Related Accession (Same CDS sequence)	NM_001271097.1 , NM_001271097.2
Accession Version	NM_001271097.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1542bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-03
Organism	Homo sapiens(human)
Product	poly(U)-binding-splicing factor PUF60 isoform e
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CN371576.1, CX163878.1, AF217197.2 and AW249619.1. Transcript Variant: This variant (5, also known as FIRdeltaexon2) lacks two alternate in-frame exons in the coding region, compared to variant 1. The encoded isoform (e) is shorter than isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR3476690.832280.1, SRR3476690.816463.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGACGG CGACCATAGC TCTCGGCACA GACTCCATCA AGATGGAGAA CGGGCAGAGC 
ACAGCCGCCA AGCTGGGGCT GCCTCCCCTG ACGCCCGAGC AGCAGGAGGC CCTTCAGAAG 
GCCAAGAAGT ACGCCATGGA GCAGAGCATC AAGAGTGTGC TGGTGAAGCA GACCATCGCG 
CACCAGCAGC AGCAGCTCAC CAACCTGCAG ATGGCGGCTC AGCGGCAGCG GGCGCTGGCC 
ATCATGTGCC GCGTCTACGT GGGCTCTATC TACTATGAGC TGGGGGAGGA CACCATCCGC 
CAGGCCTTTG CCCCCTTTGG CCCCATCAAG AGCATCGACA TGTCCTGGGA CTCCGTCACC 
ATGAAGCACA AGGGCTTTGC CTTCGTGGAG TATGAGGTCC CCGAAGCTGC ACAGCTGGCC 
TTGGAGCAGA TGAACTCGGT GATGCTGGGG GGCAGGAACA TCAAGGTGGG CAGACCCAGC 
AACATAGGGC AGGCCCAGCC CATCATAGAC CAGTTGGCTG AGGAGGCACG GGCCTTCAAC 
CGCATCTACG TGGCCTCTGT GCACCAGGAC CTCTCAGACG ATGACATCAA GAGCGTGTTT 
GAGGCCTTTG GCAAGATCAA GTCCTGCACA CTGGCCCGGG ACCCCACAAC TGGCAAGCAC 
AAGGGCTACG GCTTCATTGA GTACGAGAAG GCCCAGTCGT CCCAAGATGC TGTGTCTTCC 
ATGAACCTCT TTGACCTGGG TGGCCAGTAC TTGCGGGTGG GCAAGGCTGT CACACCGCCC 
ATGCCCCTAC TCACACCAGC CACGCCTGGA GGCCTCCCAC CTGCCGCTGC TGTGGCAGCT 
GCTGCAGCCA CTGCCAAGAT CACAGCTCAG GAAGCAGTGG CCGGAGCAGC GGTGCTGGGT 
ACCCTGGGCA CACCTGGACT GGTGTCCCCA GCACTGACCC TGGCCCAGCC CCTGGGCACT 
TTGCCCCAGG CTGTCATGGC TGCCCAGGCA CCTGGAGTCA TCACAGGTGT GACCCCAGCC 
CGTCCTCCTA TCCCGGTCAC CATCCCCTCG GTGGGAGTGG TGAACCCCAT CCTGGCCAGC 
CCTCCAACGC TGGGTCTCCT GGAGCCCAAG AAGGAGAAGG AAGAAGAGGA GCTGTTTCCC 
GAGTCAGAGC GGCCAGAGAT GCTGAGCGAG CAGGAGCACA TGAGCATCTC GGGCAGTAGC 
GCCCGACACA TGGTGATGCA GAAGCTGCTC CGCAAGCAGG AGTCTACAGT GATGGTTCTG 
CGCAACATGG TGGACCCCAA GGACATCGAT GATGACCTGG AAGGGGAGGT GACAGAGGAG 
TGTGGCAAGT TCGGGGCCGT GAACCGCGTC ATCATCTACC AAGAGAAACA AGGCGAGGAG 
GAGGATGCAG AAATCATTGT CAAGATCTTT GTGGAGTTTT CCATAGCCTC TGAGACTCAT 
AAGGCCATCC AGGCCCTCAA TGGCCGCTGG TTTGCTGGCC GCAAGGTGGT GGCTGAAGTG 
TACGACCAGG AGCGTTTTGA TAACAGTGAC CTCTCTGCGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001258026.1
CDS	84..1625
Translation	MATATIALGTDSIKMENGQSTAAKLGLPPLTPEQQEALQKAKKYAMEQSIKSVLVKQTIAHQQQQLTNLQMAAQRQRALAIMCRVYVGSIYYELGEDTIRQAFAPFGPIKSIDMSWDSVTMKHKGFAFVEYEVPEAAQLALEQMNSVMLGGRNIKVGRPSNIGQAQPIIDQLAEEARAFNRIYVASVHQDLSDDDIKSVFEAFGKIKSCTLARDPTTGKHKGYGFIEYEKAQSSQDAVSSMNLFDLGGQYLRVGKAVTPPMPLLTPATPGGLPPAAAVAAAAATAKITAQEAVAGAAVLGTLGTPGLVSPALTLAQPLGTLPQAVMAAQAPGVITGVTPARPPIPVTIPSVGVVNPILASPPTLGLLEPKKEKEEEELFPESERPEMLSEQEHMSISGSSARHMVMQKLLRKQESTVMVLRNMVDPKDIDDDLEGEVTEECGKFGAVNRVIIYQEKQGEEEDAEIIVKIFVEFSIASETHKAIQALNGRWFAGRKVVAEVYDQERFDNSDLSA

Target ORF information:

RefSeq Version	NM_001271097.1
Organism	Homo sapiens(human)
Definition	Homo sapiens poly(U) binding splicing factor 60 (PUF60), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001271097.1

ORF Insert Sequence:

ATGGCGACGG CGACCATAGC TCTCGGCACA GACTCCATCA AGATGGAGAA CGGGCAGAGC 
ACAGCCGCCA AGCTGGGGCT GCCTCCCCTG ACGCCCGAGC AGCAGGAGGC CCTTCAGAAG 
GCCAAGAAGT ACGCCATGGA GCAGAGCATC AAGAGTGTGC TGGTGAAGCA GACCATCGCG 
CACCAGCAGC AGCAGCTCAC CAACCTGCAG ATGGCGGCTC AGCGGCAGCG GGCGCTGGCC 
ATCATGTGCC GCGTCTACGT GGGCTCTATC TACTATGAGC TGGGGGAGGA CACCATCCGC 
CAGGCCTTTG CCCCCTTTGG CCCCATCAAG AGCATCGACA TGTCCTGGGA CTCCGTCACC 
ATGAAGCACA AGGGCTTTGC CTTCGTGGAG TATGAGGTCC CCGAAGCTGC ACAGCTGGCC 
TTGGAGCAGA TGAACTCGGT GATGCTGGGG GGCAGGAACA TCAAGGTGGG CAGACCCAGC 
AACATAGGGC AGGCCCAGCC CATCATAGAC CAGTTGGCTG AGGAGGCACG GGCCTTCAAC 
CGCATCTACG TGGCCTCTGT GCACCAGGAC CTCTCAGACG ATGACATCAA GAGCGTGTTT 
GAGGCCTTTG GCAAGATCAA GTCCTGCACA CTGGCCCGGG ACCCCACAAC TGGCAAGCAC 
AAGGGCTACG GCTTCATTGA GTACGAGAAG GCCCAGTCGT CCCAAGATGC TGTGTCTTCC 
ATGAACCTCT TTGACCTGGG TGGCCAGTAC TTGCGGGTGG GCAAGGCTGT CACACCGCCC 
ATGCCCCTAC TCACACCAGC CACGCCTGGA GGCCTCCCAC CTGCCGCTGC TGTGGCAGCT 
GCTGCAGCCA CTGCCAAGAT CACAGCTCAG GAAGCAGTGG CCGGAGCAGC GGTGCTGGGT 
ACCCTGGGCA CACCTGGACT GGTGTCCCCA GCACTGACCC TGGCCCAGCC CCTGGGCACT 
TTGCCCCAGG CTGTCATGGC TGCCCAGGCA CCTGGAGTCA TCACAGGTGT GACCCCAGCC 
CGTCCTCCTA TCCCGGTCAC CATCCCCTCG GTGGGAGTGG TGAACCCCAT CCTGGCCAGC 
CCTCCAACGC TGGGTCTCCT GGAGCCCAAG AAGGAGAAGG AAGAAGAGGA GCTGTTTCCC 
GAGTCAGAGC GGCCAGAGAT GCTGAGCGAG CAGGAGCACA TGAGCATCTC GGGCAGTAGC 
GCCCGACACA TGGTGATGCA GAAGCTGCTC CGCAAGCAGG AGTCTACAGT GATGGTTCTG 
CGCAACATGG TGGACCCCAA GGACATCGAT GATGACCTGG AAGGGGAGGT GACAGAGGAG 
TGTGGCAAGT TCGGGGCCGT GAACCGCGTC ATCATCTACC AAGAGAAACA AGGCGAGGAG 
GAGGATGCAG AAATCATTGT CAAGATCTTT GTGGAGTTTT CCATAGCCTC TGAGACTCAT 
AAGGCCATCC AGGCCCTCAA TGGCCGCTGG TTTGCTGGCC GCAAGGTGGT GGCTGAAGTG 
TACGACCAGG AGCGTTTTGA TAACAGTGAC CTCTCTGCGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PUF60 ( NM_001271097.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001258026.1 Homo sapiens poly(U) binding splicing factor 60 (PUF60), transcript variant 5, mRNA.

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CloneID	OHu27624
Clone ID Related Accession (Same CDS sequence)	NM_001271097.1 , NM_001271097.2
Accession Version	NM_001271097.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1542bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-27
Organism	Homo sapiens(human)
Product	poly(U)-binding-splicing factor PUF60 isoform e
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CX163878.1, AF217197.2 and AW249619.1. On May 31, 2019 this sequence version replaced NM_001271097.1. Transcript Variant: This variant (5, also known as FIRdeltaexon2) lacks two alternate in-frame exons in the coding region, compared to variant 1. The encoded isoform (e) is shorter than isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR5189661.113030.1, SRR5189655.101952.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGACGG CGACCATAGC TCTCGGCACA GACTCCATCA AGATGGAGAA CGGGCAGAGC 
ACAGCCGCCA AGCTGGGGCT GCCTCCCCTG ACGCCCGAGC AGCAGGAGGC CCTTCAGAAG 
GCCAAGAAGT ACGCCATGGA GCAGAGCATC AAGAGTGTGC TGGTGAAGCA GACCATCGCG 
CACCAGCAGC AGCAGCTCAC CAACCTGCAG ATGGCGGCTC AGCGGCAGCG GGCGCTGGCC 
ATCATGTGCC GCGTCTACGT GGGCTCTATC TACTATGAGC TGGGGGAGGA CACCATCCGC 
CAGGCCTTTG CCCCCTTTGG CCCCATCAAG AGCATCGACA TGTCCTGGGA CTCCGTCACC 
ATGAAGCACA AGGGCTTTGC CTTCGTGGAG TATGAGGTCC CCGAAGCTGC ACAGCTGGCC 
TTGGAGCAGA TGAACTCGGT GATGCTGGGG GGCAGGAACA TCAAGGTGGG CAGACCCAGC 
AACATAGGGC AGGCCCAGCC CATCATAGAC CAGTTGGCTG AGGAGGCACG GGCCTTCAAC 
CGCATCTACG TGGCCTCTGT GCACCAGGAC CTCTCAGACG ATGACATCAA GAGCGTGTTT 
GAGGCCTTTG GCAAGATCAA GTCCTGCACA CTGGCCCGGG ACCCCACAAC TGGCAAGCAC 
AAGGGCTACG GCTTCATTGA GTACGAGAAG GCCCAGTCGT CCCAAGATGC TGTGTCTTCC 
ATGAACCTCT TTGACCTGGG TGGCCAGTAC TTGCGGGTGG GCAAGGCTGT CACACCGCCC 
ATGCCCCTAC TCACACCAGC CACGCCTGGA GGCCTCCCAC CTGCCGCTGC TGTGGCAGCT 
GCTGCAGCCA CTGCCAAGAT CACAGCTCAG GAAGCAGTGG CCGGAGCAGC GGTGCTGGGT 
ACCCTGGGCA CACCTGGACT GGTGTCCCCA GCACTGACCC TGGCCCAGCC CCTGGGCACT 
TTGCCCCAGG CTGTCATGGC TGCCCAGGCA CCTGGAGTCA TCACAGGTGT GACCCCAGCC 
CGTCCTCCTA TCCCGGTCAC CATCCCCTCG GTGGGAGTGG TGAACCCCAT CCTGGCCAGC 
CCTCCAACGC TGGGTCTCCT GGAGCCCAAG AAGGAGAAGG AAGAAGAGGA GCTGTTTCCC 
GAGTCAGAGC GGCCAGAGAT GCTGAGCGAG CAGGAGCACA TGAGCATCTC GGGCAGTAGC 
GCCCGACACA TGGTGATGCA GAAGCTGCTC CGCAAGCAGG AGTCTACAGT GATGGTTCTG 
CGCAACATGG TGGACCCCAA GGACATCGAT GATGACCTGG AAGGGGAGGT GACAGAGGAG 
TGTGGCAAGT TCGGGGCCGT GAACCGCGTC ATCATCTACC AAGAGAAACA AGGCGAGGAG 
GAGGATGCAG AAATCATTGT CAAGATCTTT GTGGAGTTTT CCATAGCCTC TGAGACTCAT 
AAGGCCATCC AGGCCCTCAA TGGCCGCTGG TTTGCTGGCC GCAAGGTGGT GGCTGAAGTG 
TACGACCAGG AGCGTTTTGA TAACAGTGAC CTCTCTGCGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001258026.1
CDS	13..1554
Translation	MATATIALGTDSIKMENGQSTAAKLGLPPLTPEQQEALQKAKKYAMEQSIKSVLVKQTIAHQQQQLTNLQMAAQRQRALAIMCRVYVGSIYYELGEDTIRQAFAPFGPIKSIDMSWDSVTMKHKGFAFVEYEVPEAAQLALEQMNSVMLGGRNIKVGRPSNIGQAQPIIDQLAEEARAFNRIYVASVHQDLSDDDIKSVFEAFGKIKSCTLARDPTTGKHKGYGFIEYEKAQSSQDAVSSMNLFDLGGQYLRVGKAVTPPMPLLTPATPGGLPPAAAVAAAAATAKITAQEAVAGAAVLGTLGTPGLVSPALTLAQPLGTLPQAVMAAQAPGVITGVTPARPPIPVTIPSVGVVNPILASPPTLGLLEPKKEKEEEELFPESERPEMLSEQEHMSISGSSARHMVMQKLLRKQESTVMVLRNMVDPKDIDDDLEGEVTEECGKFGAVNRVIIYQEKQGEEEDAEIIVKIFVEFSIASETHKAIQALNGRWFAGRKVVAEVYDQERFDNSDLSA

Target ORF information:

RefSeq Version	NM_001271097.2
Organism	Homo sapiens(human)
Definition	Homo sapiens poly(U) binding splicing factor 60 (PUF60), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001271097.2

ORF Insert Sequence:

ATGGCGACGG CGACCATAGC TCTCGGCACA GACTCCATCA AGATGGAGAA CGGGCAGAGC 
ACAGCCGCCA AGCTGGGGCT GCCTCCCCTG ACGCCCGAGC AGCAGGAGGC CCTTCAGAAG 
GCCAAGAAGT ACGCCATGGA GCAGAGCATC AAGAGTGTGC TGGTGAAGCA GACCATCGCG 
CACCAGCAGC AGCAGCTCAC CAACCTGCAG ATGGCGGCTC AGCGGCAGCG GGCGCTGGCC 
ATCATGTGCC GCGTCTACGT GGGCTCTATC TACTATGAGC TGGGGGAGGA CACCATCCGC 
CAGGCCTTTG CCCCCTTTGG CCCCATCAAG AGCATCGACA TGTCCTGGGA CTCCGTCACC 
ATGAAGCACA AGGGCTTTGC CTTCGTGGAG TATGAGGTCC CCGAAGCTGC ACAGCTGGCC 
TTGGAGCAGA TGAACTCGGT GATGCTGGGG GGCAGGAACA TCAAGGTGGG CAGACCCAGC 
AACATAGGGC AGGCCCAGCC CATCATAGAC CAGTTGGCTG AGGAGGCACG GGCCTTCAAC 
CGCATCTACG TGGCCTCTGT GCACCAGGAC CTCTCAGACG ATGACATCAA GAGCGTGTTT 
GAGGCCTTTG GCAAGATCAA GTCCTGCACA CTGGCCCGGG ACCCCACAAC TGGCAAGCAC 
AAGGGCTACG GCTTCATTGA GTACGAGAAG GCCCAGTCGT CCCAAGATGC TGTGTCTTCC 
ATGAACCTCT TTGACCTGGG TGGCCAGTAC TTGCGGGTGG GCAAGGCTGT CACACCGCCC 
ATGCCCCTAC TCACACCAGC CACGCCTGGA GGCCTCCCAC CTGCCGCTGC TGTGGCAGCT 
GCTGCAGCCA CTGCCAAGAT CACAGCTCAG GAAGCAGTGG CCGGAGCAGC GGTGCTGGGT 
ACCCTGGGCA CACCTGGACT GGTGTCCCCA GCACTGACCC TGGCCCAGCC CCTGGGCACT 
TTGCCCCAGG CTGTCATGGC TGCCCAGGCA CCTGGAGTCA TCACAGGTGT GACCCCAGCC 
CGTCCTCCTA TCCCGGTCAC CATCCCCTCG GTGGGAGTGG TGAACCCCAT CCTGGCCAGC 
CCTCCAACGC TGGGTCTCCT GGAGCCCAAG AAGGAGAAGG AAGAAGAGGA GCTGTTTCCC 
GAGTCAGAGC GGCCAGAGAT GCTGAGCGAG CAGGAGCACA TGAGCATCTC GGGCAGTAGC 
GCCCGACACA TGGTGATGCA GAAGCTGCTC CGCAAGCAGG AGTCTACAGT GATGGTTCTG 
CGCAACATGG TGGACCCCAA GGACATCGAT GATGACCTGG AAGGGGAGGT GACAGAGGAG 
TGTGGCAAGT TCGGGGCCGT GAACCGCGTC ATCATCTACC AAGAGAAACA AGGCGAGGAG 
GAGGATGCAG AAATCATTGT CAAGATCTTT GTGGAGTTTT CCATAGCCTC TGAGACTCAT 
AAGGCCATCC AGGCCCTCAA TGGCCGCTGG TTTGCTGGCC GCAAGGTGGT GGCTGAAGTG 
TACGACCAGG AGCGTTTTGA TAACAGTGAC CTCTCTGCGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PUF60 ( NM_001362896.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001349825.1 Homo sapiens poly(U) binding splicing factor 60 (PUF60), transcript variant 10, mRNA.

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CloneID	OHu64963
Clone ID Related Accession (Same CDS sequence)	XM_011516929.2 , NM_001362896.2 , NM_001362895.1 , NM_001362896.1 , XM_017013238.1 , XM_017013237.1 , XM_017013234.1 , NM_001362895.2
Accession Version	NM_001362896.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1791bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-27
Organism	Homo sapiens(human)
Product	poly(U)-binding-splicing factor PUF60 isoform i
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BG771821.1, BC011265.1, AC105219.6 and AW249619.1. On May 31, 2019 this sequence version replaced NM_001362896.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.198393.1, SRR1660807.223171.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGCAGC TGAACTTGGC TGACAGTGGC CTGGTGGGGA AGGAAGCCAG AAGATGCAGA 
AGGGCCTCCC CTCCCATGGG CATGCCTTCT GCCTGGTGGC TGCAGAATGA GGCCGGCTGC 
CTCTTTGCAG CAAGGCAGGT CAATGGCCAG CAAGGAGGGG GGTCCGAGCC GGCGGCGGCG 
GCGGCAGTGG TGGCAGCGGG AGACAAATGG AAACCTCCAC AGGGCACAGA CTCCATCAAG 
ATGGAGAACG GGCAGAGCAC AGCCGCCAAG CTGGGGCTGC CTCCCCTGAC GCCCGAGCAG 
CAGGAGGCCC TTCAGAAGGC CAAGAAGTAC GCCATGGAGC AGAGCATCAA GAGTGTGCTG 
GTGAAGCAGA CCATCGCGCA CCAGCAGCAG CAGCTCACCA ACCTGCAGAT GGCAGCAGTG 
ACAATGGGCT TTGGAGATCC TCTCTCACCT TTGCAATCGA TGGCGGCTCA GCGGCAGCGG 
GCGCTGGCCA TCATGTGCCG CGTCTACGTG GGCTCTATCT ACTATGAGCT GGGGGAGGAC 
ACCATCCGCC AGGCCTTTGC CCCCTTTGGC CCCATCAAGA GCATCGACAT GTCCTGGGAC 
TCCGTCACCA TGAAGCACAA GGGCTTTGCC TTCGTGGAGT ATGAGGTCCC CGAAGCTGCA 
CAGCTGGCCT TGGAGCAGAT GAACTCGGTG ATGCTGGGGG GCAGGAACAT CAAGGTGGGC 
AGACCCAGCA ACATAGGGCA GGCCCAGCCC ATCATAGACC AGTTGGCTGA GGAGGCACGG 
GCCTTCAACC GCATCTACGT GGCCTCTGTG CACCAGGACC TCTCAGACGA TGACATCAAG 
AGCGTGTTTG AGGCCTTTGG CAAGATCAAG TCCTGCACAC TGGCCCGGGA CCCCACAACT 
GGCAAGCACA AGGGCTACGG CTTCATTGAG TACGAGAAGG CCCAGTCGTC CCAAGATGCT 
GTGTCTTCCA TGAACCTCTT TGACCTGGGT GGCCAGTACT TGCGGGTGGG CAAGGCTGTC 
ACACCGCCCA TGCCCCTACT CACACCAGCC ACGCCTGGAG GCCTCCCACC TGCCGCTGCT 
GTGGCAGCTG CTGCAGCCAC TGCCAAGATC ACAGCTCAGG AAGCAGTGGC CGGAGCAGCG 
GTGCTGGGTA CCCTGGGCAC ACCTGGACTG GTGTCCCCAG CACTGACCCT GGCCCAGCCC 
CTGGGCACTT TGCCCCAGGC TGTCATGGCT GCCCAGGCAC CTGGAGTCAT CACAGGTGTG 
ACCCCAGCCC GTCCTCCTAT CCCGGTCACC ATCCCCTCGG TGGGAGTGGT GAACCCCATC 
CTGGCCAGCC CTCCAACGCT GGGTCTCCTG GAGCCCAAGA AGGAGAAGGA AGAAGAGGAG 
CTGTTTCCCG AGTCAGAGCG GCCAGAGATG CTGAGCGAGC AGGAGCACAT GAGCATCTCG 
GGCAGTAGCG CCCGACACAT GGTGATGCAG AAGCTGCTCC GCAAGCAGGA GTCTACAGTG 
ATGGTTCTGC GCAACATGGT GGACCCCAAG GACATCGATG ATGACCTGGA AGGGGAGGTG 
ACAGAGGAGT GTGGCAAGTT CGGGGCCGTG AACCGCGTCA TCATCTACCA AGAGAAACAA 
GGCGAGGAGG AGGATGCAGA AATCATTGTC AAGATCTTTG TGGAGTTTTC CATAGCCTCT 
GAGACTCATA AGGCCATCCA GGCCCTCAAT GGCCGCTGGT TTGCTGGCCG CAAGGTGGTG 
GCTGAAGTGT ACGACCAGGA GCGTTTTGAT AACAGTGACC TCTCTGCGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001349825.1
CDS	239..2029
Translation	MEQLNLADSGLVGKEARRCRRASPPMGMPSAWWLQNEAGCLFAARQVNGQQGGGSEPAAAAAVVAAGDKWKPPQGTDSIKMENGQSTAAKLGLPPLTPEQQEALQKAKKYAMEQSIKSVLVKQTIAHQQQQLTNLQMAAVTMGFGDPLSPLQSMAAQRQRALAIMCRVYVGSIYYELGEDTIRQAFAPFGPIKSIDMSWDSVTMKHKGFAFVEYEVPEAAQLALEQMNSVMLGGRNIKVGRPSNIGQAQPIIDQLAEEARAFNRIYVASVHQDLSDDDIKSVFEAFGKIKSCTLARDPTTGKHKGYGFIEYEKAQSSQDAVSSMNLFDLGGQYLRVGKAVTPPMPLLTPATPGGLPPAAAVAAAAATAKITAQEAVAGAAVLGTLGTPGLVSPALTLAQPLGTLPQAVMAAQAPGVITGVTPARPPIPVTIPSVGVVNPILASPPTLGLLEPKKEKEEEELFPESERPEMLSEQEHMSISGSSARHMVMQKLLRKQESTVMVLRNMVDPKDIDDDLEGEVTEECGKFGAVNRVIIYQEKQGEEEDAEIIVKIFVEFSIASETHKAIQALNGRWFAGRKVVAEVYDQERFDNSDLSA

Target ORF information:

RefSeq Version	NM_001362896.2
Organism	Homo sapiens(human)
Definition	Homo sapiens poly(U) binding splicing factor 60 (PUF60), transcript variant 10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001362896.2

ORF Insert Sequence:

ATGGAGCAGC TGAACTTGGC TGACAGTGGC CTGGTGGGGA AGGAAGCCAG AAGATGCAGA 
AGGGCCTCCC CTCCCATGGG CATGCCTTCT GCCTGGTGGC TGCAGAATGA GGCCGGCTGC 
CTCTTTGCAG CAAGGCAGGT CAATGGCCAG CAAGGAGGGG GGTCCGAGCC GGCGGCGGCG 
GCGGCAGTGG TGGCAGCGGG AGACAAATGG AAACCTCCAC AGGGCACAGA CTCCATCAAG 
ATGGAGAACG GGCAGAGCAC AGCCGCCAAG CTGGGGCTGC CTCCCCTGAC GCCCGAGCAG 
CAGGAGGCCC TTCAGAAGGC CAAGAAGTAC GCCATGGAGC AGAGCATCAA GAGTGTGCTG 
GTGAAGCAGA CCATCGCGCA CCAGCAGCAG CAGCTCACCA ACCTGCAGAT GGCAGCAGTG 
ACAATGGGCT TTGGAGATCC TCTCTCACCT TTGCAATCGA TGGCGGCTCA GCGGCAGCGG 
GCGCTGGCCA TCATGTGCCG CGTCTACGTG GGCTCTATCT ACTATGAGCT GGGGGAGGAC 
ACCATCCGCC AGGCCTTTGC CCCCTTTGGC CCCATCAAGA GCATCGACAT GTCCTGGGAC 
TCCGTCACCA TGAAGCACAA GGGCTTTGCC TTCGTGGAGT ATGAGGTCCC CGAAGCTGCA 
CAGCTGGCCT TGGAGCAGAT GAACTCGGTG ATGCTGGGGG GCAGGAACAT CAAGGTGGGC 
AGACCCAGCA ACATAGGGCA GGCCCAGCCC ATCATAGACC AGTTGGCTGA GGAGGCACGG 
GCCTTCAACC GCATCTACGT GGCCTCTGTG CACCAGGACC TCTCAGACGA TGACATCAAG 
AGCGTGTTTG AGGCCTTTGG CAAGATCAAG TCCTGCACAC TGGCCCGGGA CCCCACAACT 
GGCAAGCACA AGGGCTACGG CTTCATTGAG TACGAGAAGG CCCAGTCGTC CCAAGATGCT 
GTGTCTTCCA TGAACCTCTT TGACCTGGGT GGCCAGTACT TGCGGGTGGG CAAGGCTGTC 
ACACCGCCCA TGCCCCTACT CACACCAGCC ACGCCTGGAG GCCTCCCACC TGCCGCTGCT 
GTGGCAGCTG CTGCAGCCAC TGCCAAGATC ACAGCTCAGG AAGCAGTGGC CGGAGCAGCG 
GTGCTGGGTA CCCTGGGCAC ACCTGGACTG GTGTCCCCAG CACTGACCCT GGCCCAGCCC 
CTGGGCACTT TGCCCCAGGC TGTCATGGCT GCCCAGGCAC CTGGAGTCAT CACAGGTGTG 
ACCCCAGCCC GTCCTCCTAT CCCGGTCACC ATCCCCTCGG TGGGAGTGGT GAACCCCATC 
CTGGCCAGCC CTCCAACGCT GGGTCTCCTG GAGCCCAAGA AGGAGAAGGA AGAAGAGGAG 
CTGTTTCCCG AGTCAGAGCG GCCAGAGATG CTGAGCGAGC AGGAGCACAT GAGCATCTCG 
GGCAGTAGCG CCCGACACAT GGTGATGCAG AAGCTGCTCC GCAAGCAGGA GTCTACAGTG 
ATGGTTCTGC GCAACATGGT GGACCCCAAG GACATCGATG ATGACCTGGA AGGGGAGGTG 
ACAGAGGAGT GTGGCAAGTT CGGGGCCGTG AACCGCGTCA TCATCTACCA AGAGAAACAA 
GGCGAGGAGG AGGATGCAGA AATCATTGTC AAGATCTTTG TGGAGTTTTC CATAGCCTCT 
GAGACTCATA AGGCCATCCA GGCCCTCAAT GGCCGCTGGT TTGCTGGCCG CAAGGTGGTG 
GCTGAAGTGT ACGACCAGGA GCGTTTTGAT AACAGTGACC TCTCTGCGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PUF60 ( NM_001362895.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001349824.1 Homo sapiens poly(U) binding splicing factor 60 (PUF60), transcript variant 9, mRNA.

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CloneID	OHu64963
Clone ID Related Accession (Same CDS sequence)	XM_011516929.2 , NM_001362896.2 , NM_001362895.1 , NM_001362896.1 , XM_017013238.1 , XM_017013237.1 , XM_017013234.1 , NM_001362895.2
Accession Version	NM_001362895.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1791bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-10-21
Organism	Homo sapiens(human)
Product	poly(U)-binding-splicing factor PUF60 isoform i
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC234917.3, BG771821.1, BC011265.1, AC105219.6 and AW249619.1. On May 3, 2018 this sequence version replaced XM_017013238.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.57786.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGCAGC TGAACTTGGC TGACAGTGGC CTGGTGGGGA AGGAAGCCAG AAGATGCAGA 
AGGGCCTCCC CTCCCATGGG CATGCCTTCT GCCTGGTGGC TGCAGAATGA GGCCGGCTGC 
CTCTTTGCAG CAAGGCAGGT CAATGGCCAG CAAGGAGGGG GGTCCGAGCC GGCGGCGGCG 
GCGGCAGTGG TGGCAGCGGG AGACAAATGG AAACCTCCAC AGGGCACAGA CTCCATCAAG 
ATGGAGAACG GGCAGAGCAC AGCCGCCAAG CTGGGGCTGC CTCCCCTGAC GCCCGAGCAG 
CAGGAGGCCC TTCAGAAGGC CAAGAAGTAC GCCATGGAGC AGAGCATCAA GAGTGTGCTG 
GTGAAGCAGA CCATCGCGCA CCAGCAGCAG CAGCTCACCA ACCTGCAGAT GGCAGCAGTG 
ACAATGGGCT TTGGAGATCC TCTCTCACCT TTGCAATCGA TGGCGGCTCA GCGGCAGCGG 
GCGCTGGCCA TCATGTGCCG CGTCTACGTG GGCTCTATCT ACTATGAGCT GGGGGAGGAC 
ACCATCCGCC AGGCCTTTGC CCCCTTTGGC CCCATCAAGA GCATCGACAT GTCCTGGGAC 
TCCGTCACCA TGAAGCACAA GGGCTTTGCC TTCGTGGAGT ATGAGGTCCC CGAAGCTGCA 
CAGCTGGCCT TGGAGCAGAT GAACTCGGTG ATGCTGGGGG GCAGGAACAT CAAGGTGGGC 
AGACCCAGCA ACATAGGGCA GGCCCAGCCC ATCATAGACC AGTTGGCTGA GGAGGCACGG 
GCCTTCAACC GCATCTACGT GGCCTCTGTG CACCAGGACC TCTCAGACGA TGACATCAAG 
AGCGTGTTTG AGGCCTTTGG CAAGATCAAG TCCTGCACAC TGGCCCGGGA CCCCACAACT 
GGCAAGCACA AGGGCTACGG CTTCATTGAG TACGAGAAGG CCCAGTCGTC CCAAGATGCT 
GTGTCTTCCA TGAACCTCTT TGACCTGGGT GGCCAGTACT TGCGGGTGGG CAAGGCTGTC 
ACACCGCCCA TGCCCCTACT CACACCAGCC ACGCCTGGAG GCCTCCCACC TGCCGCTGCT 
GTGGCAGCTG CTGCAGCCAC TGCCAAGATC ACAGCTCAGG AAGCAGTGGC CGGAGCAGCG 
GTGCTGGGTA CCCTGGGCAC ACCTGGACTG GTGTCCCCAG CACTGACCCT GGCCCAGCCC 
CTGGGCACTT TGCCCCAGGC TGTCATGGCT GCCCAGGCAC CTGGAGTCAT CACAGGTGTG 
ACCCCAGCCC GTCCTCCTAT CCCGGTCACC ATCCCCTCGG TGGGAGTGGT GAACCCCATC 
CTGGCCAGCC CTCCAACGCT GGGTCTCCTG GAGCCCAAGA AGGAGAAGGA AGAAGAGGAG 
CTGTTTCCCG AGTCAGAGCG GCCAGAGATG CTGAGCGAGC AGGAGCACAT GAGCATCTCG 
GGCAGTAGCG CCCGACACAT GGTGATGCAG AAGCTGCTCC GCAAGCAGGA GTCTACAGTG 
ATGGTTCTGC GCAACATGGT GGACCCCAAG GACATCGATG ATGACCTGGA AGGGGAGGTG 
ACAGAGGAGT GTGGCAAGTT CGGGGCCGTG AACCGCGTCA TCATCTACCA AGAGAAACAA 
GGCGAGGAGG AGGATGCAGA AATCATTGTC AAGATCTTTG TGGAGTTTTC CATAGCCTCT 
GAGACTCATA AGGCCATCCA GGCCCTCAAT GGCCGCTGGT TTGCTGGCCG CAAGGTGGTG 
GCTGAAGTGT ACGACCAGGA GCGTTTTGAT AACAGTGACC TCTCTGCGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001349824.1
CDS	144..1934
Translation	MEQLNLADSGLVGKEARRCRRASPPMGMPSAWWLQNEAGCLFAARQVNGQQGGGSEPAAAAAVVAAGDKWKPPQGTDSIKMENGQSTAAKLGLPPLTPEQQEALQKAKKYAMEQSIKSVLVKQTIAHQQQQLTNLQMAAVTMGFGDPLSPLQSMAAQRQRALAIMCRVYVGSIYYELGEDTIRQAFAPFGPIKSIDMSWDSVTMKHKGFAFVEYEVPEAAQLALEQMNSVMLGGRNIKVGRPSNIGQAQPIIDQLAEEARAFNRIYVASVHQDLSDDDIKSVFEAFGKIKSCTLARDPTTGKHKGYGFIEYEKAQSSQDAVSSMNLFDLGGQYLRVGKAVTPPMPLLTPATPGGLPPAAAVAAAAATAKITAQEAVAGAAVLGTLGTPGLVSPALTLAQPLGTLPQAVMAAQAPGVITGVTPARPPIPVTIPSVGVVNPILASPPTLGLLEPKKEKEEEELFPESERPEMLSEQEHMSISGSSARHMVMQKLLRKQESTVMVLRNMVDPKDIDDDLEGEVTEECGKFGAVNRVIIYQEKQGEEEDAEIIVKIFVEFSIASETHKAIQALNGRWFAGRKVVAEVYDQERFDNSDLSA

Target ORF information:

RefSeq Version	NM_001362895.1
Organism	Homo sapiens(human)
Definition	Homo sapiens poly(U) binding splicing factor 60 (PUF60), transcript variant 9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001362895.1

ORF Insert Sequence:

ATGGAGCAGC TGAACTTGGC TGACAGTGGC CTGGTGGGGA AGGAAGCCAG AAGATGCAGA 
AGGGCCTCCC CTCCCATGGG CATGCCTTCT GCCTGGTGGC TGCAGAATGA GGCCGGCTGC 
CTCTTTGCAG CAAGGCAGGT CAATGGCCAG CAAGGAGGGG GGTCCGAGCC GGCGGCGGCG 
GCGGCAGTGG TGGCAGCGGG AGACAAATGG AAACCTCCAC AGGGCACAGA CTCCATCAAG 
ATGGAGAACG GGCAGAGCAC AGCCGCCAAG CTGGGGCTGC CTCCCCTGAC GCCCGAGCAG 
CAGGAGGCCC TTCAGAAGGC CAAGAAGTAC GCCATGGAGC AGAGCATCAA GAGTGTGCTG 
GTGAAGCAGA CCATCGCGCA CCAGCAGCAG CAGCTCACCA ACCTGCAGAT GGCAGCAGTG 
ACAATGGGCT TTGGAGATCC TCTCTCACCT TTGCAATCGA TGGCGGCTCA GCGGCAGCGG 
GCGCTGGCCA TCATGTGCCG CGTCTACGTG GGCTCTATCT ACTATGAGCT GGGGGAGGAC 
ACCATCCGCC AGGCCTTTGC CCCCTTTGGC CCCATCAAGA GCATCGACAT GTCCTGGGAC 
TCCGTCACCA TGAAGCACAA GGGCTTTGCC TTCGTGGAGT ATGAGGTCCC CGAAGCTGCA 
CAGCTGGCCT TGGAGCAGAT GAACTCGGTG ATGCTGGGGG GCAGGAACAT CAAGGTGGGC 
AGACCCAGCA ACATAGGGCA GGCCCAGCCC ATCATAGACC AGTTGGCTGA GGAGGCACGG 
GCCTTCAACC GCATCTACGT GGCCTCTGTG CACCAGGACC TCTCAGACGA TGACATCAAG 
AGCGTGTTTG AGGCCTTTGG CAAGATCAAG TCCTGCACAC TGGCCCGGGA CCCCACAACT 
GGCAAGCACA AGGGCTACGG CTTCATTGAG TACGAGAAGG CCCAGTCGTC CCAAGATGCT 
GTGTCTTCCA TGAACCTCTT TGACCTGGGT GGCCAGTACT TGCGGGTGGG CAAGGCTGTC 
ACACCGCCCA TGCCCCTACT CACACCAGCC ACGCCTGGAG GCCTCCCACC TGCCGCTGCT 
GTGGCAGCTG CTGCAGCCAC TGCCAAGATC ACAGCTCAGG AAGCAGTGGC CGGAGCAGCG 
GTGCTGGGTA CCCTGGGCAC ACCTGGACTG GTGTCCCCAG CACTGACCCT GGCCCAGCCC 
CTGGGCACTT TGCCCCAGGC TGTCATGGCT GCCCAGGCAC CTGGAGTCAT CACAGGTGTG 
ACCCCAGCCC GTCCTCCTAT CCCGGTCACC ATCCCCTCGG TGGGAGTGGT GAACCCCATC 
CTGGCCAGCC CTCCAACGCT GGGTCTCCTG GAGCCCAAGA AGGAGAAGGA AGAAGAGGAG 
CTGTTTCCCG AGTCAGAGCG GCCAGAGATG CTGAGCGAGC AGGAGCACAT GAGCATCTCG 
GGCAGTAGCG CCCGACACAT GGTGATGCAG AAGCTGCTCC GCAAGCAGGA GTCTACAGTG 
ATGGTTCTGC GCAACATGGT GGACCCCAAG GACATCGATG ATGACCTGGA AGGGGAGGTG 
ACAGAGGAGT GTGGCAAGTT CGGGGCCGTG AACCGCGTCA TCATCTACCA AGAGAAACAA 
GGCGAGGAGG AGGATGCAGA AATCATTGTC AAGATCTTTG TGGAGTTTTC CATAGCCTCT 
GAGACTCATA AGGCCATCCA GGCCCTCAAT GGCCGCTGGT TTGCTGGCCG CAAGGTGGTG 
GCTGAAGTGT ACGACCAGGA GCGTTTTGAT AACAGTGACC TCTCTGCGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PUF60 ( NM_001362896.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001349825.1 Homo sapiens poly(U) binding splicing factor 60 (PUF60), transcript variant 10, mRNA.

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CloneID	OHu64963
Clone ID Related Accession (Same CDS sequence)	XM_011516929.2 , NM_001362896.2 , NM_001362895.1 , NM_001362896.1 , XM_017013238.1 , XM_017013237.1 , XM_017013234.1 , NM_001362895.2
Accession Version	NM_001362896.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1791bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-10-21
Organism	Homo sapiens(human)
Product	poly(U)-binding-splicing factor PUF60 isoform i
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC234917.3, BG771821.1, BC011265.1, AC105219.6 and AW249619.1. On May 3, 2018 this sequence version replaced XM_017013237.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.223171.1, SRR1660803.198393.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGCAGC TGAACTTGGC TGACAGTGGC CTGGTGGGGA AGGAAGCCAG AAGATGCAGA 
AGGGCCTCCC CTCCCATGGG CATGCCTTCT GCCTGGTGGC TGCAGAATGA GGCCGGCTGC 
CTCTTTGCAG CAAGGCAGGT CAATGGCCAG CAAGGAGGGG GGTCCGAGCC GGCGGCGGCG 
GCGGCAGTGG TGGCAGCGGG AGACAAATGG AAACCTCCAC AGGGCACAGA CTCCATCAAG 
ATGGAGAACG GGCAGAGCAC AGCCGCCAAG CTGGGGCTGC CTCCCCTGAC GCCCGAGCAG 
CAGGAGGCCC TTCAGAAGGC CAAGAAGTAC GCCATGGAGC AGAGCATCAA GAGTGTGCTG 
GTGAAGCAGA CCATCGCGCA CCAGCAGCAG CAGCTCACCA ACCTGCAGAT GGCAGCAGTG 
ACAATGGGCT TTGGAGATCC TCTCTCACCT TTGCAATCGA TGGCGGCTCA GCGGCAGCGG 
GCGCTGGCCA TCATGTGCCG CGTCTACGTG GGCTCTATCT ACTATGAGCT GGGGGAGGAC 
ACCATCCGCC AGGCCTTTGC CCCCTTTGGC CCCATCAAGA GCATCGACAT GTCCTGGGAC 
TCCGTCACCA TGAAGCACAA GGGCTTTGCC TTCGTGGAGT ATGAGGTCCC CGAAGCTGCA 
CAGCTGGCCT TGGAGCAGAT GAACTCGGTG ATGCTGGGGG GCAGGAACAT CAAGGTGGGC 
AGACCCAGCA ACATAGGGCA GGCCCAGCCC ATCATAGACC AGTTGGCTGA GGAGGCACGG 
GCCTTCAACC GCATCTACGT GGCCTCTGTG CACCAGGACC TCTCAGACGA TGACATCAAG 
AGCGTGTTTG AGGCCTTTGG CAAGATCAAG TCCTGCACAC TGGCCCGGGA CCCCACAACT 
GGCAAGCACA AGGGCTACGG CTTCATTGAG TACGAGAAGG CCCAGTCGTC CCAAGATGCT 
GTGTCTTCCA TGAACCTCTT TGACCTGGGT GGCCAGTACT TGCGGGTGGG CAAGGCTGTC 
ACACCGCCCA TGCCCCTACT CACACCAGCC ACGCCTGGAG GCCTCCCACC TGCCGCTGCT 
GTGGCAGCTG CTGCAGCCAC TGCCAAGATC ACAGCTCAGG AAGCAGTGGC CGGAGCAGCG 
GTGCTGGGTA CCCTGGGCAC ACCTGGACTG GTGTCCCCAG CACTGACCCT GGCCCAGCCC 
CTGGGCACTT TGCCCCAGGC TGTCATGGCT GCCCAGGCAC CTGGAGTCAT CACAGGTGTG 
ACCCCAGCCC GTCCTCCTAT CCCGGTCACC ATCCCCTCGG TGGGAGTGGT GAACCCCATC 
CTGGCCAGCC CTCCAACGCT GGGTCTCCTG GAGCCCAAGA AGGAGAAGGA AGAAGAGGAG 
CTGTTTCCCG AGTCAGAGCG GCCAGAGATG CTGAGCGAGC AGGAGCACAT GAGCATCTCG 
GGCAGTAGCG CCCGACACAT GGTGATGCAG AAGCTGCTCC GCAAGCAGGA GTCTACAGTG 
ATGGTTCTGC GCAACATGGT GGACCCCAAG GACATCGATG ATGACCTGGA AGGGGAGGTG 
ACAGAGGAGT GTGGCAAGTT CGGGGCCGTG AACCGCGTCA TCATCTACCA AGAGAAACAA 
GGCGAGGAGG AGGATGCAGA AATCATTGTC AAGATCTTTG TGGAGTTTTC CATAGCCTCT 
GAGACTCATA AGGCCATCCA GGCCCTCAAT GGCCGCTGGT TTGCTGGCCG CAAGGTGGTG 
GCTGAAGTGT ACGACCAGGA GCGTTTTGAT AACAGTGACC TCTCTGCGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001349825.1
CDS	307..2097
Translation	MEQLNLADSGLVGKEARRCRRASPPMGMPSAWWLQNEAGCLFAARQVNGQQGGGSEPAAAAAVVAAGDKWKPPQGTDSIKMENGQSTAAKLGLPPLTPEQQEALQKAKKYAMEQSIKSVLVKQTIAHQQQQLTNLQMAAVTMGFGDPLSPLQSMAAQRQRALAIMCRVYVGSIYYELGEDTIRQAFAPFGPIKSIDMSWDSVTMKHKGFAFVEYEVPEAAQLALEQMNSVMLGGRNIKVGRPSNIGQAQPIIDQLAEEARAFNRIYVASVHQDLSDDDIKSVFEAFGKIKSCTLARDPTTGKHKGYGFIEYEKAQSSQDAVSSMNLFDLGGQYLRVGKAVTPPMPLLTPATPGGLPPAAAVAAAAATAKITAQEAVAGAAVLGTLGTPGLVSPALTLAQPLGTLPQAVMAAQAPGVITGVTPARPPIPVTIPSVGVVNPILASPPTLGLLEPKKEKEEEELFPESERPEMLSEQEHMSISGSSARHMVMQKLLRKQESTVMVLRNMVDPKDIDDDLEGEVTEECGKFGAVNRVIIYQEKQGEEEDAEIIVKIFVEFSIASETHKAIQALNGRWFAGRKVVAEVYDQERFDNSDLSA

Target ORF information:

RefSeq Version	NM_001362896.1
Organism	Homo sapiens(human)
Definition	Homo sapiens poly(U) binding splicing factor 60 (PUF60), transcript variant 10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001362896.1

ORF Insert Sequence:

ATGGAGCAGC TGAACTTGGC TGACAGTGGC CTGGTGGGGA AGGAAGCCAG AAGATGCAGA 
AGGGCCTCCC CTCCCATGGG CATGCCTTCT GCCTGGTGGC TGCAGAATGA GGCCGGCTGC 
CTCTTTGCAG CAAGGCAGGT CAATGGCCAG CAAGGAGGGG GGTCCGAGCC GGCGGCGGCG 
GCGGCAGTGG TGGCAGCGGG AGACAAATGG AAACCTCCAC AGGGCACAGA CTCCATCAAG 
ATGGAGAACG GGCAGAGCAC AGCCGCCAAG CTGGGGCTGC CTCCCCTGAC GCCCGAGCAG 
CAGGAGGCCC TTCAGAAGGC CAAGAAGTAC GCCATGGAGC AGAGCATCAA GAGTGTGCTG 
GTGAAGCAGA CCATCGCGCA CCAGCAGCAG CAGCTCACCA ACCTGCAGAT GGCAGCAGTG 
ACAATGGGCT TTGGAGATCC TCTCTCACCT TTGCAATCGA TGGCGGCTCA GCGGCAGCGG 
GCGCTGGCCA TCATGTGCCG CGTCTACGTG GGCTCTATCT ACTATGAGCT GGGGGAGGAC 
ACCATCCGCC AGGCCTTTGC CCCCTTTGGC CCCATCAAGA GCATCGACAT GTCCTGGGAC 
TCCGTCACCA TGAAGCACAA GGGCTTTGCC TTCGTGGAGT ATGAGGTCCC CGAAGCTGCA 
CAGCTGGCCT TGGAGCAGAT GAACTCGGTG ATGCTGGGGG GCAGGAACAT CAAGGTGGGC 
AGACCCAGCA ACATAGGGCA GGCCCAGCCC ATCATAGACC AGTTGGCTGA GGAGGCACGG 
GCCTTCAACC GCATCTACGT GGCCTCTGTG CACCAGGACC TCTCAGACGA TGACATCAAG 
AGCGTGTTTG AGGCCTTTGG CAAGATCAAG TCCTGCACAC TGGCCCGGGA CCCCACAACT 
GGCAAGCACA AGGGCTACGG CTTCATTGAG TACGAGAAGG CCCAGTCGTC CCAAGATGCT 
GTGTCTTCCA TGAACCTCTT TGACCTGGGT GGCCAGTACT TGCGGGTGGG CAAGGCTGTC 
ACACCGCCCA TGCCCCTACT CACACCAGCC ACGCCTGGAG GCCTCCCACC TGCCGCTGCT 
GTGGCAGCTG CTGCAGCCAC TGCCAAGATC ACAGCTCAGG AAGCAGTGGC CGGAGCAGCG 
GTGCTGGGTA CCCTGGGCAC ACCTGGACTG GTGTCCCCAG CACTGACCCT GGCCCAGCCC 
CTGGGCACTT TGCCCCAGGC TGTCATGGCT GCCCAGGCAC CTGGAGTCAT CACAGGTGTG 
ACCCCAGCCC GTCCTCCTAT CCCGGTCACC ATCCCCTCGG TGGGAGTGGT GAACCCCATC 
CTGGCCAGCC CTCCAACGCT GGGTCTCCTG GAGCCCAAGA AGGAGAAGGA AGAAGAGGAG 
CTGTTTCCCG AGTCAGAGCG GCCAGAGATG CTGAGCGAGC AGGAGCACAT GAGCATCTCG 
GGCAGTAGCG CCCGACACAT GGTGATGCAG AAGCTGCTCC GCAAGCAGGA GTCTACAGTG 
ATGGTTCTGC GCAACATGGT GGACCCCAAG GACATCGATG ATGACCTGGA AGGGGAGGTG 
ACAGAGGAGT GTGGCAAGTT CGGGGCCGTG AACCGCGTCA TCATCTACCA AGAGAAACAA 
GGCGAGGAGG AGGATGCAGA AATCATTGTC AAGATCTTTG TGGAGTTTTC CATAGCCTCT 
GAGACTCATA AGGCCATCCA GGCCCTCAAT GGCCGCTGGT TTGCTGGCCG CAAGGTGGTG 
GCTGAAGTGT ACGACCAGGA GCGTTTTGAT AACAGTGACC TCTCTGCGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PUF60 ( NM_001362895.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001349824.1 Homo sapiens poly(U) binding splicing factor 60 (PUF60), transcript variant 9, mRNA.

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CloneID	OHu64963
Clone ID Related Accession (Same CDS sequence)	XM_011516929.2 , NM_001362896.2 , NM_001362895.1 , NM_001362896.1 , XM_017013238.1 , XM_017013237.1 , XM_017013234.1 , NM_001362895.2
Accession Version	NM_001362895.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1791bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-27
Organism	Homo sapiens(human)
Product	poly(U)-binding-splicing factor PUF60 isoform i
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BG771821.1, BC011265.1, AC105219.6 and AW249619.1. On May 31, 2019 this sequence version replaced NM_001362895.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.57786.1, SRR7346977.97285.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGCAGC TGAACTTGGC TGACAGTGGC CTGGTGGGGA AGGAAGCCAG AAGATGCAGA 
AGGGCCTCCC CTCCCATGGG CATGCCTTCT GCCTGGTGGC TGCAGAATGA GGCCGGCTGC 
CTCTTTGCAG CAAGGCAGGT CAATGGCCAG CAAGGAGGGG GGTCCGAGCC GGCGGCGGCG 
GCGGCAGTGG TGGCAGCGGG AGACAAATGG AAACCTCCAC AGGGCACAGA CTCCATCAAG 
ATGGAGAACG GGCAGAGCAC AGCCGCCAAG CTGGGGCTGC CTCCCCTGAC GCCCGAGCAG 
CAGGAGGCCC TTCAGAAGGC CAAGAAGTAC GCCATGGAGC AGAGCATCAA GAGTGTGCTG 
GTGAAGCAGA CCATCGCGCA CCAGCAGCAG CAGCTCACCA ACCTGCAGAT GGCAGCAGTG 
ACAATGGGCT TTGGAGATCC TCTCTCACCT TTGCAATCGA TGGCGGCTCA GCGGCAGCGG 
GCGCTGGCCA TCATGTGCCG CGTCTACGTG GGCTCTATCT ACTATGAGCT GGGGGAGGAC 
ACCATCCGCC AGGCCTTTGC CCCCTTTGGC CCCATCAAGA GCATCGACAT GTCCTGGGAC 
TCCGTCACCA TGAAGCACAA GGGCTTTGCC TTCGTGGAGT ATGAGGTCCC CGAAGCTGCA 
CAGCTGGCCT TGGAGCAGAT GAACTCGGTG ATGCTGGGGG GCAGGAACAT CAAGGTGGGC 
AGACCCAGCA ACATAGGGCA GGCCCAGCCC ATCATAGACC AGTTGGCTGA GGAGGCACGG 
GCCTTCAACC GCATCTACGT GGCCTCTGTG CACCAGGACC TCTCAGACGA TGACATCAAG 
AGCGTGTTTG AGGCCTTTGG CAAGATCAAG TCCTGCACAC TGGCCCGGGA CCCCACAACT 
GGCAAGCACA AGGGCTACGG CTTCATTGAG TACGAGAAGG CCCAGTCGTC CCAAGATGCT 
GTGTCTTCCA TGAACCTCTT TGACCTGGGT GGCCAGTACT TGCGGGTGGG CAAGGCTGTC 
ACACCGCCCA TGCCCCTACT CACACCAGCC ACGCCTGGAG GCCTCCCACC TGCCGCTGCT 
GTGGCAGCTG CTGCAGCCAC TGCCAAGATC ACAGCTCAGG AAGCAGTGGC CGGAGCAGCG 
GTGCTGGGTA CCCTGGGCAC ACCTGGACTG GTGTCCCCAG CACTGACCCT GGCCCAGCCC 
CTGGGCACTT TGCCCCAGGC TGTCATGGCT GCCCAGGCAC CTGGAGTCAT CACAGGTGTG 
ACCCCAGCCC GTCCTCCTAT CCCGGTCACC ATCCCCTCGG TGGGAGTGGT GAACCCCATC 
CTGGCCAGCC CTCCAACGCT GGGTCTCCTG GAGCCCAAGA AGGAGAAGGA AGAAGAGGAG 
CTGTTTCCCG AGTCAGAGCG GCCAGAGATG CTGAGCGAGC AGGAGCACAT GAGCATCTCG 
GGCAGTAGCG CCCGACACAT GGTGATGCAG AAGCTGCTCC GCAAGCAGGA GTCTACAGTG 
ATGGTTCTGC GCAACATGGT GGACCCCAAG GACATCGATG ATGACCTGGA AGGGGAGGTG 
ACAGAGGAGT GTGGCAAGTT CGGGGCCGTG AACCGCGTCA TCATCTACCA AGAGAAACAA 
GGCGAGGAGG AGGATGCAGA AATCATTGTC AAGATCTTTG TGGAGTTTTC CATAGCCTCT 
GAGACTCATA AGGCCATCCA GGCCCTCAAT GGCCGCTGGT TTGCTGGCCG CAAGGTGGTG 
GCTGAAGTGT ACGACCAGGA GCGTTTTGAT AACAGTGACC TCTCTGCGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001349824.1
CDS	76..1866
Translation	MEQLNLADSGLVGKEARRCRRASPPMGMPSAWWLQNEAGCLFAARQVNGQQGGGSEPAAAAAVVAAGDKWKPPQGTDSIKMENGQSTAAKLGLPPLTPEQQEALQKAKKYAMEQSIKSVLVKQTIAHQQQQLTNLQMAAVTMGFGDPLSPLQSMAAQRQRALAIMCRVYVGSIYYELGEDTIRQAFAPFGPIKSIDMSWDSVTMKHKGFAFVEYEVPEAAQLALEQMNSVMLGGRNIKVGRPSNIGQAQPIIDQLAEEARAFNRIYVASVHQDLSDDDIKSVFEAFGKIKSCTLARDPTTGKHKGYGFIEYEKAQSSQDAVSSMNLFDLGGQYLRVGKAVTPPMPLLTPATPGGLPPAAAVAAAAATAKITAQEAVAGAAVLGTLGTPGLVSPALTLAQPLGTLPQAVMAAQAPGVITGVTPARPPIPVTIPSVGVVNPILASPPTLGLLEPKKEKEEEELFPESERPEMLSEQEHMSISGSSARHMVMQKLLRKQESTVMVLRNMVDPKDIDDDLEGEVTEECGKFGAVNRVIIYQEKQGEEEDAEIIVKIFVEFSIASETHKAIQALNGRWFAGRKVVAEVYDQERFDNSDLSA

Target ORF information:

RefSeq Version	NM_001362895.2
Organism	Homo sapiens(human)
Definition	Homo sapiens poly(U) binding splicing factor 60 (PUF60), transcript variant 9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001362895.2

ORF Insert Sequence:

ATGGAGCAGC TGAACTTGGC TGACAGTGGC CTGGTGGGGA AGGAAGCCAG AAGATGCAGA 
AGGGCCTCCC CTCCCATGGG CATGCCTTCT GCCTGGTGGC TGCAGAATGA GGCCGGCTGC 
CTCTTTGCAG CAAGGCAGGT CAATGGCCAG CAAGGAGGGG GGTCCGAGCC GGCGGCGGCG 
GCGGCAGTGG TGGCAGCGGG AGACAAATGG AAACCTCCAC AGGGCACAGA CTCCATCAAG 
ATGGAGAACG GGCAGAGCAC AGCCGCCAAG CTGGGGCTGC CTCCCCTGAC GCCCGAGCAG 
CAGGAGGCCC TTCAGAAGGC CAAGAAGTAC GCCATGGAGC AGAGCATCAA GAGTGTGCTG 
GTGAAGCAGA CCATCGCGCA CCAGCAGCAG CAGCTCACCA ACCTGCAGAT GGCAGCAGTG 
ACAATGGGCT TTGGAGATCC TCTCTCACCT TTGCAATCGA TGGCGGCTCA GCGGCAGCGG 
GCGCTGGCCA TCATGTGCCG CGTCTACGTG GGCTCTATCT ACTATGAGCT GGGGGAGGAC 
ACCATCCGCC AGGCCTTTGC CCCCTTTGGC CCCATCAAGA GCATCGACAT GTCCTGGGAC 
TCCGTCACCA TGAAGCACAA GGGCTTTGCC TTCGTGGAGT ATGAGGTCCC CGAAGCTGCA 
CAGCTGGCCT TGGAGCAGAT GAACTCGGTG ATGCTGGGGG GCAGGAACAT CAAGGTGGGC 
AGACCCAGCA ACATAGGGCA GGCCCAGCCC ATCATAGACC AGTTGGCTGA GGAGGCACGG 
GCCTTCAACC GCATCTACGT GGCCTCTGTG CACCAGGACC TCTCAGACGA TGACATCAAG 
AGCGTGTTTG AGGCCTTTGG CAAGATCAAG TCCTGCACAC TGGCCCGGGA CCCCACAACT 
GGCAAGCACA AGGGCTACGG CTTCATTGAG TACGAGAAGG CCCAGTCGTC CCAAGATGCT 
GTGTCTTCCA TGAACCTCTT TGACCTGGGT GGCCAGTACT TGCGGGTGGG CAAGGCTGTC 
ACACCGCCCA TGCCCCTACT CACACCAGCC ACGCCTGGAG GCCTCCCACC TGCCGCTGCT 
GTGGCAGCTG CTGCAGCCAC TGCCAAGATC ACAGCTCAGG AAGCAGTGGC CGGAGCAGCG 
GTGCTGGGTA CCCTGGGCAC ACCTGGACTG GTGTCCCCAG CACTGACCCT GGCCCAGCCC 
CTGGGCACTT TGCCCCAGGC TGTCATGGCT GCCCAGGCAC CTGGAGTCAT CACAGGTGTG 
ACCCCAGCCC GTCCTCCTAT CCCGGTCACC ATCCCCTCGG TGGGAGTGGT GAACCCCATC 
CTGGCCAGCC CTCCAACGCT GGGTCTCCTG GAGCCCAAGA AGGAGAAGGA AGAAGAGGAG 
CTGTTTCCCG AGTCAGAGCG GCCAGAGATG CTGAGCGAGC AGGAGCACAT GAGCATCTCG 
GGCAGTAGCG CCCGACACAT GGTGATGCAG AAGCTGCTCC GCAAGCAGGA GTCTACAGTG 
ATGGTTCTGC GCAACATGGT GGACCCCAAG GACATCGATG ATGACCTGGA AGGGGAGGTG 
ACAGAGGAGT GTGGCAAGTT CGGGGCCGTG AACCGCGTCA TCATCTACCA AGAGAAACAA 
GGCGAGGAGG AGGATGCAGA AATCATTGTC AAGATCTTTG TGGAGTTTTC CATAGCCTCT 
GAGACTCATA AGGCCATCCA GGCCCTCAAT GGCCGCTGGT TTGCTGGCCG CAAGGTGGTG 
GCTGAAGTGT ACGACCAGGA GCGTTTTGAT AACAGTGACC TCTCTGCGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PUF60 ( NM_001362897.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001349826.1 Homo sapiens poly(U) binding splicing factor 60 (PUF60), transcript variant 11, mRNA.

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CloneID	OHu64964
Clone ID Related Accession (Same CDS sequence)	NM_001362897.1 , XM_017013236.1 , NM_001362897.2 , XM_011516930.2
Accession Version	NM_001362897.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1740bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-10-21
Organism	Homo sapiens(human)
Product	poly(U)-binding-splicing factor PUF60 isoform j
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC234917.3, BG771821.1, BQ421738.1, BC011265.1 and AW249619.1. On May 3, 2018 this sequence version replaced XM_011516930.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.156133.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGCAGC TGAACTTGGC TGACAGTGGC CTGGTGGGGA AGGAAGCCAG AAGATGCAGA 
AGGGCCTCCC CTCCCATGGG CATGCCTTCT GCCTGGTGGC TGCAGAATGA GGCCGGCTGC 
CTCTTTGCAG CAAGGCAGGT CAATGGCCAG CAAGGAGGGG GGTCCGAGCC GGCGGCGGCG 
GCGGCAGTGG TGGCAGCGGG AGACAAATGG AAACCTCCAC AGGGCACAGA CTCCATCAAG 
ATGGAGAACG GGCAGAGCAC AGCCGCCAAG CTGGGGCTGC CTCCCCTGAC GCCCGAGCAG 
CAGGAGGCCC TTCAGAAGGC CAAGAAGTAC GCCATGGAGC AGAGCATCAA GAGTGTGCTG 
GTGAAGCAGA CCATCGCGCA CCAGCAGCAG CAGCTCACCA ACCTGCAGAT GGCGGCTCAG 
CGGCAGCGGG CGCTGGCCAT CATGTGCCGC GTCTACGTGG GCTCTATCTA CTATGAGCTG 
GGGGAGGACA CCATCCGCCA GGCCTTTGCC CCCTTTGGCC CCATCAAGAG CATCGACATG 
TCCTGGGACT CCGTCACCAT GAAGCACAAG GGCTTTGCCT TCGTGGAGTA TGAGGTCCCC 
GAAGCTGCAC AGCTGGCCTT GGAGCAGATG AACTCGGTGA TGCTGGGGGG CAGGAACATC 
AAGGTGGGCA GACCCAGCAA CATAGGGCAG GCCCAGCCCA TCATAGACCA GTTGGCTGAG 
GAGGCACGGG CCTTCAACCG CATCTACGTG GCCTCTGTGC ACCAGGACCT CTCAGACGAT 
GACATCAAGA GCGTGTTTGA GGCCTTTGGC AAGATCAAGT CCTGCACACT GGCCCGGGAC 
CCCACAACTG GCAAGCACAA GGGCTACGGC TTCATTGAGT ACGAGAAGGC CCAGTCGTCC 
CAAGATGCTG TGTCTTCCAT GAACCTCTTT GACCTGGGTG GCCAGTACTT GCGGGTGGGC 
AAGGCTGTCA CACCGCCCAT GCCCCTACTC ACACCAGCCA CGCCTGGAGG CCTCCCACCT 
GCCGCTGCTG TGGCAGCTGC TGCAGCCACT GCCAAGATCA CAGCTCAGGA AGCAGTGGCC 
GGAGCAGCGG TGCTGGGTAC CCTGGGCACA CCTGGACTGG TGTCCCCAGC ACTGACCCTG 
GCCCAGCCCC TGGGCACTTT GCCCCAGGCT GTCATGGCTG CCCAGGCACC TGGAGTCATC 
ACAGGTGTGA CCCCAGCCCG TCCTCCTATC CCGGTCACCA TCCCCTCGGT GGGAGTGGTG 
AACCCCATCC TGGCCAGCCC TCCAACGCTG GGTCTCCTGG AGCCCAAGAA GGAGAAGGAA 
GAAGAGGAGC TGTTTCCCGA GTCAGAGCGG CCAGAGATGC TGAGCGAGCA GGAGCACATG 
AGCATCTCGG GCAGTAGCGC CCGACACATG GTGATGCAGA AGCTGCTCCG CAAGCAGGAG 
TCTACAGTGA TGGTTCTGCG CAACATGGTG GACCCCAAGG ACATCGATGA TGACCTGGAA 
GGGGAGGTGA CAGAGGAGTG TGGCAAGTTC GGGGCCGTGA ACCGCGTCAT CATCTACCAA 
GAGAAACAAG GCGAGGAGGA GGATGCAGAA ATCATTGTCA AGATCTTTGT GGAGTTTTCC 
ATAGCCTCTG AGACTCATAA GGCCATCCAG GCCCTCAATG GCCGCTGGTT TGCTGGCCGC 
AAGGTGGTGG CTGAAGTGTA CGACCAGGAG CGTTTTGATA ACAGTGACCT CTCTGCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001349826.1
CDS	144..1883
Translation	MEQLNLADSGLVGKEARRCRRASPPMGMPSAWWLQNEAGCLFAARQVNGQQGGGSEPAAAAAVVAAGDKWKPPQGTDSIKMENGQSTAAKLGLPPLTPEQQEALQKAKKYAMEQSIKSVLVKQTIAHQQQQLTNLQMAAQRQRALAIMCRVYVGSIYYELGEDTIRQAFAPFGPIKSIDMSWDSVTMKHKGFAFVEYEVPEAAQLALEQMNSVMLGGRNIKVGRPSNIGQAQPIIDQLAEEARAFNRIYVASVHQDLSDDDIKSVFEAFGKIKSCTLARDPTTGKHKGYGFIEYEKAQSSQDAVSSMNLFDLGGQYLRVGKAVTPPMPLLTPATPGGLPPAAAVAAAAATAKITAQEAVAGAAVLGTLGTPGLVSPALTLAQPLGTLPQAVMAAQAPGVITGVTPARPPIPVTIPSVGVVNPILASPPTLGLLEPKKEKEEEELFPESERPEMLSEQEHMSISGSSARHMVMQKLLRKQESTVMVLRNMVDPKDIDDDLEGEVTEECGKFGAVNRVIIYQEKQGEEEDAEIIVKIFVEFSIASETHKAIQALNGRWFAGRKVVAEVYDQERFDNSDLSA

Target ORF information:

RefSeq Version	NM_001362897.1
Organism	Homo sapiens(human)
Definition	Homo sapiens poly(U) binding splicing factor 60 (PUF60), transcript variant 11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001362897.1

ORF Insert Sequence:

ATGGAGCAGC TGAACTTGGC TGACAGTGGC CTGGTGGGGA AGGAAGCCAG AAGATGCAGA 
AGGGCCTCCC CTCCCATGGG CATGCCTTCT GCCTGGTGGC TGCAGAATGA GGCCGGCTGC 
CTCTTTGCAG CAAGGCAGGT CAATGGCCAG CAAGGAGGGG GGTCCGAGCC GGCGGCGGCG 
GCGGCAGTGG TGGCAGCGGG AGACAAATGG AAACCTCCAC AGGGCACAGA CTCCATCAAG 
ATGGAGAACG GGCAGAGCAC AGCCGCCAAG CTGGGGCTGC CTCCCCTGAC GCCCGAGCAG 
CAGGAGGCCC TTCAGAAGGC CAAGAAGTAC GCCATGGAGC AGAGCATCAA GAGTGTGCTG 
GTGAAGCAGA CCATCGCGCA CCAGCAGCAG CAGCTCACCA ACCTGCAGAT GGCGGCTCAG 
CGGCAGCGGG CGCTGGCCAT CATGTGCCGC GTCTACGTGG GCTCTATCTA CTATGAGCTG 
GGGGAGGACA CCATCCGCCA GGCCTTTGCC CCCTTTGGCC CCATCAAGAG CATCGACATG 
TCCTGGGACT CCGTCACCAT GAAGCACAAG GGCTTTGCCT TCGTGGAGTA TGAGGTCCCC 
GAAGCTGCAC AGCTGGCCTT GGAGCAGATG AACTCGGTGA TGCTGGGGGG CAGGAACATC 
AAGGTGGGCA GACCCAGCAA CATAGGGCAG GCCCAGCCCA TCATAGACCA GTTGGCTGAG 
GAGGCACGGG CCTTCAACCG CATCTACGTG GCCTCTGTGC ACCAGGACCT CTCAGACGAT 
GACATCAAGA GCGTGTTTGA GGCCTTTGGC AAGATCAAGT CCTGCACACT GGCCCGGGAC 
CCCACAACTG GCAAGCACAA GGGCTACGGC TTCATTGAGT ACGAGAAGGC CCAGTCGTCC 
CAAGATGCTG TGTCTTCCAT GAACCTCTTT GACCTGGGTG GCCAGTACTT GCGGGTGGGC 
AAGGCTGTCA CACCGCCCAT GCCCCTACTC ACACCAGCCA CGCCTGGAGG CCTCCCACCT 
GCCGCTGCTG TGGCAGCTGC TGCAGCCACT GCCAAGATCA CAGCTCAGGA AGCAGTGGCC 
GGAGCAGCGG TGCTGGGTAC CCTGGGCACA CCTGGACTGG TGTCCCCAGC ACTGACCCTG 
GCCCAGCCCC TGGGCACTTT GCCCCAGGCT GTCATGGCTG CCCAGGCACC TGGAGTCATC 
ACAGGTGTGA CCCCAGCCCG TCCTCCTATC CCGGTCACCA TCCCCTCGGT GGGAGTGGTG 
AACCCCATCC TGGCCAGCCC TCCAACGCTG GGTCTCCTGG AGCCCAAGAA GGAGAAGGAA 
GAAGAGGAGC TGTTTCCCGA GTCAGAGCGG CCAGAGATGC TGAGCGAGCA GGAGCACATG 
AGCATCTCGG GCAGTAGCGC CCGACACATG GTGATGCAGA AGCTGCTCCG CAAGCAGGAG 
TCTACAGTGA TGGTTCTGCG CAACATGGTG GACCCCAAGG ACATCGATGA TGACCTGGAA 
GGGGAGGTGA CAGAGGAGTG TGGCAAGTTC GGGGCCGTGA ACCGCGTCAT CATCTACCAA 
GAGAAACAAG GCGAGGAGGA GGATGCAGAA ATCATTGTCA AGATCTTTGT GGAGTTTTCC 
ATAGCCTCTG AGACTCATAA GGCCATCCAG GCCCTCAATG GCCGCTGGTT TGCTGGCCGC 
AAGGTGGTGG CTGAAGTGTA CGACCAGGAG CGTTTTGATA ACAGTGACCT CTCTGCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PUF60 ( NM_001362897.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001349826.1 Homo sapiens poly(U) binding splicing factor 60 (PUF60), transcript variant 11, mRNA.

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CloneID	OHu64964
Clone ID Related Accession (Same CDS sequence)	NM_001362897.1 , XM_017013236.1 , NM_001362897.2 , XM_011516930.2
Accession Version	NM_001362897.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1740bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-27
Organism	Homo sapiens(human)
Product	poly(U)-binding-splicing factor PUF60 isoform j
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BQ421738.1, BC011265.1 and AW249619.1. On May 31, 2019 this sequence version replaced NM_001362897.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.156133.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGCAGC TGAACTTGGC TGACAGTGGC CTGGTGGGGA AGGAAGCCAG AAGATGCAGA 
AGGGCCTCCC CTCCCATGGG CATGCCTTCT GCCTGGTGGC TGCAGAATGA GGCCGGCTGC 
CTCTTTGCAG CAAGGCAGGT CAATGGCCAG CAAGGAGGGG GGTCCGAGCC GGCGGCGGCG 
GCGGCAGTGG TGGCAGCGGG AGACAAATGG AAACCTCCAC AGGGCACAGA CTCCATCAAG 
ATGGAGAACG GGCAGAGCAC AGCCGCCAAG CTGGGGCTGC CTCCCCTGAC GCCCGAGCAG 
CAGGAGGCCC TTCAGAAGGC CAAGAAGTAC GCCATGGAGC AGAGCATCAA GAGTGTGCTG 
GTGAAGCAGA CCATCGCGCA CCAGCAGCAG CAGCTCACCA ACCTGCAGAT GGCGGCTCAG 
CGGCAGCGGG CGCTGGCCAT CATGTGCCGC GTCTACGTGG GCTCTATCTA CTATGAGCTG 
GGGGAGGACA CCATCCGCCA GGCCTTTGCC CCCTTTGGCC CCATCAAGAG CATCGACATG 
TCCTGGGACT CCGTCACCAT GAAGCACAAG GGCTTTGCCT TCGTGGAGTA TGAGGTCCCC 
GAAGCTGCAC AGCTGGCCTT GGAGCAGATG AACTCGGTGA TGCTGGGGGG CAGGAACATC 
AAGGTGGGCA GACCCAGCAA CATAGGGCAG GCCCAGCCCA TCATAGACCA GTTGGCTGAG 
GAGGCACGGG CCTTCAACCG CATCTACGTG GCCTCTGTGC ACCAGGACCT CTCAGACGAT 
GACATCAAGA GCGTGTTTGA GGCCTTTGGC AAGATCAAGT CCTGCACACT GGCCCGGGAC 
CCCACAACTG GCAAGCACAA GGGCTACGGC TTCATTGAGT ACGAGAAGGC CCAGTCGTCC 
CAAGATGCTG TGTCTTCCAT GAACCTCTTT GACCTGGGTG GCCAGTACTT GCGGGTGGGC 
AAGGCTGTCA CACCGCCCAT GCCCCTACTC ACACCAGCCA CGCCTGGAGG CCTCCCACCT 
GCCGCTGCTG TGGCAGCTGC TGCAGCCACT GCCAAGATCA CAGCTCAGGA AGCAGTGGCC 
GGAGCAGCGG TGCTGGGTAC CCTGGGCACA CCTGGACTGG TGTCCCCAGC ACTGACCCTG 
GCCCAGCCCC TGGGCACTTT GCCCCAGGCT GTCATGGCTG CCCAGGCACC TGGAGTCATC 
ACAGGTGTGA CCCCAGCCCG TCCTCCTATC CCGGTCACCA TCCCCTCGGT GGGAGTGGTG 
AACCCCATCC TGGCCAGCCC TCCAACGCTG GGTCTCCTGG AGCCCAAGAA GGAGAAGGAA 
GAAGAGGAGC TGTTTCCCGA GTCAGAGCGG CCAGAGATGC TGAGCGAGCA GGAGCACATG 
AGCATCTCGG GCAGTAGCGC CCGACACATG GTGATGCAGA AGCTGCTCCG CAAGCAGGAG 
TCTACAGTGA TGGTTCTGCG CAACATGGTG GACCCCAAGG ACATCGATGA TGACCTGGAA 
GGGGAGGTGA CAGAGGAGTG TGGCAAGTTC GGGGCCGTGA ACCGCGTCAT CATCTACCAA 
GAGAAACAAG GCGAGGAGGA GGATGCAGAA ATCATTGTCA AGATCTTTGT GGAGTTTTCC 
ATAGCCTCTG AGACTCATAA GGCCATCCAG GCCCTCAATG GCCGCTGGTT TGCTGGCCGC 
AAGGTGGTGG CTGAAGTGTA CGACCAGGAG CGTTTTGATA ACAGTGACCT CTCTGCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001349826.1
CDS	76..1815
Translation	MEQLNLADSGLVGKEARRCRRASPPMGMPSAWWLQNEAGCLFAARQVNGQQGGGSEPAAAAAVVAAGDKWKPPQGTDSIKMENGQSTAAKLGLPPLTPEQQEALQKAKKYAMEQSIKSVLVKQTIAHQQQQLTNLQMAAQRQRALAIMCRVYVGSIYYELGEDTIRQAFAPFGPIKSIDMSWDSVTMKHKGFAFVEYEVPEAAQLALEQMNSVMLGGRNIKVGRPSNIGQAQPIIDQLAEEARAFNRIYVASVHQDLSDDDIKSVFEAFGKIKSCTLARDPTTGKHKGYGFIEYEKAQSSQDAVSSMNLFDLGGQYLRVGKAVTPPMPLLTPATPGGLPPAAAVAAAAATAKITAQEAVAGAAVLGTLGTPGLVSPALTLAQPLGTLPQAVMAAQAPGVITGVTPARPPIPVTIPSVGVVNPILASPPTLGLLEPKKEKEEEELFPESERPEMLSEQEHMSISGSSARHMVMQKLLRKQESTVMVLRNMVDPKDIDDDLEGEVTEECGKFGAVNRVIIYQEKQGEEEDAEIIVKIFVEFSIASETHKAIQALNGRWFAGRKVVAEVYDQERFDNSDLSA

Target ORF information:

RefSeq Version	NM_001362897.2
Organism	Homo sapiens(human)
Definition	Homo sapiens poly(U) binding splicing factor 60 (PUF60), transcript variant 11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001362897.2

ORF Insert Sequence:

ATGGAGCAGC TGAACTTGGC TGACAGTGGC CTGGTGGGGA AGGAAGCCAG AAGATGCAGA 
AGGGCCTCCC CTCCCATGGG CATGCCTTCT GCCTGGTGGC TGCAGAATGA GGCCGGCTGC 
CTCTTTGCAG CAAGGCAGGT CAATGGCCAG CAAGGAGGGG GGTCCGAGCC GGCGGCGGCG 
GCGGCAGTGG TGGCAGCGGG AGACAAATGG AAACCTCCAC AGGGCACAGA CTCCATCAAG 
ATGGAGAACG GGCAGAGCAC AGCCGCCAAG CTGGGGCTGC CTCCCCTGAC GCCCGAGCAG 
CAGGAGGCCC TTCAGAAGGC CAAGAAGTAC GCCATGGAGC AGAGCATCAA GAGTGTGCTG 
GTGAAGCAGA CCATCGCGCA CCAGCAGCAG CAGCTCACCA ACCTGCAGAT GGCGGCTCAG 
CGGCAGCGGG CGCTGGCCAT CATGTGCCGC GTCTACGTGG GCTCTATCTA CTATGAGCTG 
GGGGAGGACA CCATCCGCCA GGCCTTTGCC CCCTTTGGCC CCATCAAGAG CATCGACATG 
TCCTGGGACT CCGTCACCAT GAAGCACAAG GGCTTTGCCT TCGTGGAGTA TGAGGTCCCC 
GAAGCTGCAC AGCTGGCCTT GGAGCAGATG AACTCGGTGA TGCTGGGGGG CAGGAACATC 
AAGGTGGGCA GACCCAGCAA CATAGGGCAG GCCCAGCCCA TCATAGACCA GTTGGCTGAG 
GAGGCACGGG CCTTCAACCG CATCTACGTG GCCTCTGTGC ACCAGGACCT CTCAGACGAT 
GACATCAAGA GCGTGTTTGA GGCCTTTGGC AAGATCAAGT CCTGCACACT GGCCCGGGAC 
CCCACAACTG GCAAGCACAA GGGCTACGGC TTCATTGAGT ACGAGAAGGC CCAGTCGTCC 
CAAGATGCTG TGTCTTCCAT GAACCTCTTT GACCTGGGTG GCCAGTACTT GCGGGTGGGC 
AAGGCTGTCA CACCGCCCAT GCCCCTACTC ACACCAGCCA CGCCTGGAGG CCTCCCACCT 
GCCGCTGCTG TGGCAGCTGC TGCAGCCACT GCCAAGATCA CAGCTCAGGA AGCAGTGGCC 
GGAGCAGCGG TGCTGGGTAC CCTGGGCACA CCTGGACTGG TGTCCCCAGC ACTGACCCTG 
GCCCAGCCCC TGGGCACTTT GCCCCAGGCT GTCATGGCTG CCCAGGCACC TGGAGTCATC 
ACAGGTGTGA CCCCAGCCCG TCCTCCTATC CCGGTCACCA TCCCCTCGGT GGGAGTGGTG 
AACCCCATCC TGGCCAGCCC TCCAACGCTG GGTCTCCTGG AGCCCAAGAA GGAGAAGGAA 
GAAGAGGAGC TGTTTCCCGA GTCAGAGCGG CCAGAGATGC TGAGCGAGCA GGAGCACATG 
AGCATCTCGG GCAGTAGCGC CCGACACATG GTGATGCAGA AGCTGCTCCG CAAGCAGGAG 
TCTACAGTGA TGGTTCTGCG CAACATGGTG GACCCCAAGG ACATCGATGA TGACCTGGAA 
GGGGAGGTGA CAGAGGAGTG TGGCAAGTTC GGGGCCGTGA ACCGCGTCAT CATCTACCAA 
GAGAAACAAG GCGAGGAGGA GGATGCAGAA ATCATTGTCA AGATCTTTGT GGAGTTTTCC 
ATAGCCTCTG AGACTCATAA GGCCATCCAG GCCCTCAATG GCCGCTGGTT TGCTGGCCGC 
AAGGTGGTGG CTGAAGTGTA CGACCAGGAG CGTTTTGATA ACAGTGACCT CTCTGCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PUF60 ( XM_011516929.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011515231.1 Homo sapiens poly(U) binding splicing factor 60KDa (PUF60), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu64963
Clone ID Related Accession (Same CDS sequence)	XM_011516929.2 , NM_001362896.2 , NM_001362895.1 , NM_001362896.1 , XM_017013238.1 , XM_017013237.1 , XM_017013234.1 , NM_001362895.2
Accession Version	XM_011516929.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1791bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	poly(U)-binding-splicing factor PUF60 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000008.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011516929.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCAGC TGAACTTGGC TGACAGTGGC CTGGTGGGGA AGGAAGCCAG AAGATGCAGA 
AGGGCCTCCC CTCCCATGGG CATGCCTTCT GCCTGGTGGC TGCAGAATGA GGCCGGCTGC 
CTCTTTGCAG CAAGGCAGGT CAATGGCCAG CAAGGAGGGG GGTCCGAGCC GGCGGCGGCG 
GCGGCAGTGG TGGCAGCGGG AGACAAATGG AAACCTCCAC AGGGCACAGA CTCCATCAAG 
ATGGAGAACG GGCAGAGCAC AGCCGCCAAG CTGGGGCTGC CTCCCCTGAC GCCCGAGCAG 
CAGGAGGCCC TTCAGAAGGC CAAGAAGTAC GCCATGGAGC AGAGCATCAA GAGTGTGCTG 
GTGAAGCAGA CCATCGCGCA CCAGCAGCAG CAGCTCACCA ACCTGCAGAT GGCAGCAGTG 
ACAATGGGCT TTGGAGATCC TCTCTCACCT TTGCAATCGA TGGCGGCTCA GCGGCAGCGG 
GCGCTGGCCA TCATGTGCCG CGTCTACGTG GGCTCTATCT ACTATGAGCT GGGGGAGGAC 
ACCATCCGCC AGGCCTTTGC CCCCTTTGGC CCCATCAAGA GCATCGACAT GTCCTGGGAC 
TCCGTCACCA TGAAGCACAA GGGCTTTGCC TTCGTGGAGT ATGAGGTCCC CGAAGCTGCA 
CAGCTGGCCT TGGAGCAGAT GAACTCGGTG ATGCTGGGGG GCAGGAACAT CAAGGTGGGC 
AGACCCAGCA ACATAGGGCA GGCCCAGCCC ATCATAGACC AGTTGGCTGA GGAGGCACGG 
GCCTTCAACC GCATCTACGT GGCCTCTGTG CACCAGGACC TCTCAGACGA TGACATCAAG 
AGCGTGTTTG AGGCCTTTGG CAAGATCAAG TCCTGCACAC TGGCCCGGGA CCCCACAACT 
GGCAAGCACA AGGGCTACGG CTTCATTGAG TACGAGAAGG CCCAGTCGTC CCAAGATGCT 
GTGTCTTCCA TGAACCTCTT TGACCTGGGT GGCCAGTACT TGCGGGTGGG CAAGGCTGTC 
ACACCGCCCA TGCCCCTACT CACACCAGCC ACGCCTGGAG GCCTCCCACC TGCCGCTGCT 
GTGGCAGCTG CTGCAGCCAC TGCCAAGATC ACAGCTCAGG AAGCAGTGGC CGGAGCAGCG 
GTGCTGGGTA CCCTGGGCAC ACCTGGACTG GTGTCCCCAG CACTGACCCT GGCCCAGCCC 
CTGGGCACTT TGCCCCAGGC TGTCATGGCT GCCCAGGCAC CTGGAGTCAT CACAGGTGTG 
ACCCCAGCCC GTCCTCCTAT CCCGGTCACC ATCCCCTCGG TGGGAGTGGT GAACCCCATC 
CTGGCCAGCC CTCCAACGCT GGGTCTCCTG GAGCCCAAGA AGGAGAAGGA AGAAGAGGAG 
CTGTTTCCCG AGTCAGAGCG GCCAGAGATG CTGAGCGAGC AGGAGCACAT GAGCATCTCG 
GGCAGTAGCG CCCGACACAT GGTGATGCAG AAGCTGCTCC GCAAGCAGGA GTCTACAGTG 
ATGGTTCTGC GCAACATGGT GGACCCCAAG GACATCGATG ATGACCTGGA AGGGGAGGTG 
ACAGAGGAGT GTGGCAAGTT CGGGGCCGTG AACCGCGTCA TCATCTACCA AGAGAAACAA 
GGCGAGGAGG AGGATGCAGA AATCATTGTC AAGATCTTTG TGGAGTTTTC CATAGCCTCT 
GAGACTCATA AGGCCATCCA GGCCCTCAAT GGCCGCTGGT TTGCTGGCCG CAAGGTGGTG 
GCTGAAGTGT ACGACCAGGA GCGTTTTGAT AACAGTGACC TCTCTGCGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011515231.1
CDS	172..1962
Translation	MEQLNLADSGLVGKEARRCRRASPPMGMPSAWWLQNEAGCLFAARQVNGQQGGGSEPAAAAAVVAAGDKWKPPQGTDSIKMENGQSTAAKLGLPPLTPEQQEALQKAKKYAMEQSIKSVLVKQTIAHQQQQLTNLQMAAVTMGFGDPLSPLQSMAAQRQRALAIMCRVYVGSIYYELGEDTIRQAFAPFGPIKSIDMSWDSVTMKHKGFAFVEYEVPEAAQLALEQMNSVMLGGRNIKVGRPSNIGQAQPIIDQLAEEARAFNRIYVASVHQDLSDDDIKSVFEAFGKIKSCTLARDPTTGKHKGYGFIEYEKAQSSQDAVSSMNLFDLGGQYLRVGKAVTPPMPLLTPATPGGLPPAAAVAAAAATAKITAQEAVAGAAVLGTLGTPGLVSPALTLAQPLGTLPQAVMAAQAPGVITGVTPARPPIPVTIPSVGVVNPILASPPTLGLLEPKKEKEEEELFPESERPEMLSEQEHMSISGSSARHMVMQKLLRKQESTVMVLRNMVDPKDIDDDLEGEVTEECGKFGAVNRVIIYQEKQGEEEDAEIIVKIFVEFSIASETHKAIQALNGRWFAGRKVVAEVYDQERFDNSDLSA

Target ORF information:

RefSeq Version	XM_011516929.2
Organism	Homo sapiens(human)
Definition	Homo sapiens poly(U) binding splicing factor 60KDa (PUF60), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011516929.2

ORF Insert Sequence:

ATGGAGCAGC TGAACTTGGC TGACAGTGGC CTGGTGGGGA AGGAAGCCAG AAGATGCAGA 
AGGGCCTCCC CTCCCATGGG CATGCCTTCT GCCTGGTGGC TGCAGAATGA GGCCGGCTGC 
CTCTTTGCAG CAAGGCAGGT CAATGGCCAG CAAGGAGGGG GGTCCGAGCC GGCGGCGGCG 
GCGGCAGTGG TGGCAGCGGG AGACAAATGG AAACCTCCAC AGGGCACAGA CTCCATCAAG 
ATGGAGAACG GGCAGAGCAC AGCCGCCAAG CTGGGGCTGC CTCCCCTGAC GCCCGAGCAG 
CAGGAGGCCC TTCAGAAGGC CAAGAAGTAC GCCATGGAGC AGAGCATCAA GAGTGTGCTG 
GTGAAGCAGA CCATCGCGCA CCAGCAGCAG CAGCTCACCA ACCTGCAGAT GGCAGCAGTG 
ACAATGGGCT TTGGAGATCC TCTCTCACCT TTGCAATCGA TGGCGGCTCA GCGGCAGCGG 
GCGCTGGCCA TCATGTGCCG CGTCTACGTG GGCTCTATCT ACTATGAGCT GGGGGAGGAC 
ACCATCCGCC AGGCCTTTGC CCCCTTTGGC CCCATCAAGA GCATCGACAT GTCCTGGGAC 
TCCGTCACCA TGAAGCACAA GGGCTTTGCC TTCGTGGAGT ATGAGGTCCC CGAAGCTGCA 
CAGCTGGCCT TGGAGCAGAT GAACTCGGTG ATGCTGGGGG GCAGGAACAT CAAGGTGGGC 
AGACCCAGCA ACATAGGGCA GGCCCAGCCC ATCATAGACC AGTTGGCTGA GGAGGCACGG 
GCCTTCAACC GCATCTACGT GGCCTCTGTG CACCAGGACC TCTCAGACGA TGACATCAAG 
AGCGTGTTTG AGGCCTTTGG CAAGATCAAG TCCTGCACAC TGGCCCGGGA CCCCACAACT 
GGCAAGCACA AGGGCTACGG CTTCATTGAG TACGAGAAGG CCCAGTCGTC CCAAGATGCT 
GTGTCTTCCA TGAACCTCTT TGACCTGGGT GGCCAGTACT TGCGGGTGGG CAAGGCTGTC 
ACACCGCCCA TGCCCCTACT CACACCAGCC ACGCCTGGAG GCCTCCCACC TGCCGCTGCT 
GTGGCAGCTG CTGCAGCCAC TGCCAAGATC ACAGCTCAGG AAGCAGTGGC CGGAGCAGCG 
GTGCTGGGTA CCCTGGGCAC ACCTGGACTG GTGTCCCCAG CACTGACCCT GGCCCAGCCC 
CTGGGCACTT TGCCCCAGGC TGTCATGGCT GCCCAGGCAC CTGGAGTCAT CACAGGTGTG 
ACCCCAGCCC GTCCTCCTAT CCCGGTCACC ATCCCCTCGG TGGGAGTGGT GAACCCCATC 
CTGGCCAGCC CTCCAACGCT GGGTCTCCTG GAGCCCAAGA AGGAGAAGGA AGAAGAGGAG 
CTGTTTCCCG AGTCAGAGCG GCCAGAGATG CTGAGCGAGC AGGAGCACAT GAGCATCTCG 
GGCAGTAGCG CCCGACACAT GGTGATGCAG AAGCTGCTCC GCAAGCAGGA GTCTACAGTG 
ATGGTTCTGC GCAACATGGT GGACCCCAAG GACATCGATG ATGACCTGGA AGGGGAGGTG 
ACAGAGGAGT GTGGCAAGTT CGGGGCCGTG AACCGCGTCA TCATCTACCA AGAGAAACAA 
GGCGAGGAGG AGGATGCAGA AATCATTGTC AAGATCTTTG TGGAGTTTTC CATAGCCTCT 
GAGACTCATA AGGCCATCCA GGCCCTCAAT GGCCGCTGGT TTGCTGGCCG CAAGGTGGTG 
GCTGAAGTGT ACGACCAGGA GCGTTTTGAT AACAGTGACC TCTCTGCGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PUF60 ( XM_017013238.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016868727.1 Homo sapiens poly(U) binding splicing factor 60KDa (PUF60), transcript variant X7, mRNA.

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CloneID	OHu64963
Clone ID Related Accession (Same CDS sequence)	XM_011516929.2 , NM_001362896.2 , NM_001362895.1 , NM_001362896.1 , XM_017013238.1 , XM_017013237.1 , XM_017013234.1 , NM_001362895.2
Accession Version	XM_017013238.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1791bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	poly(U)-binding-splicing factor PUF60 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000008.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCAGC TGAACTTGGC TGACAGTGGC CTGGTGGGGA AGGAAGCCAG AAGATGCAGA 
AGGGCCTCCC CTCCCATGGG CATGCCTTCT GCCTGGTGGC TGCAGAATGA GGCCGGCTGC 
CTCTTTGCAG CAAGGCAGGT CAATGGCCAG CAAGGAGGGG GGTCCGAGCC GGCGGCGGCG 
GCGGCAGTGG TGGCAGCGGG AGACAAATGG AAACCTCCAC AGGGCACAGA CTCCATCAAG 
ATGGAGAACG GGCAGAGCAC AGCCGCCAAG CTGGGGCTGC CTCCCCTGAC GCCCGAGCAG 
CAGGAGGCCC TTCAGAAGGC CAAGAAGTAC GCCATGGAGC AGAGCATCAA GAGTGTGCTG 
GTGAAGCAGA CCATCGCGCA CCAGCAGCAG CAGCTCACCA ACCTGCAGAT GGCAGCAGTG 
ACAATGGGCT TTGGAGATCC TCTCTCACCT TTGCAATCGA TGGCGGCTCA GCGGCAGCGG 
GCGCTGGCCA TCATGTGCCG CGTCTACGTG GGCTCTATCT ACTATGAGCT GGGGGAGGAC 
ACCATCCGCC AGGCCTTTGC CCCCTTTGGC CCCATCAAGA GCATCGACAT GTCCTGGGAC 
TCCGTCACCA TGAAGCACAA GGGCTTTGCC TTCGTGGAGT ATGAGGTCCC CGAAGCTGCA 
CAGCTGGCCT TGGAGCAGAT GAACTCGGTG ATGCTGGGGG GCAGGAACAT CAAGGTGGGC 
AGACCCAGCA ACATAGGGCA GGCCCAGCCC ATCATAGACC AGTTGGCTGA GGAGGCACGG 
GCCTTCAACC GCATCTACGT GGCCTCTGTG CACCAGGACC TCTCAGACGA TGACATCAAG 
AGCGTGTTTG AGGCCTTTGG CAAGATCAAG TCCTGCACAC TGGCCCGGGA CCCCACAACT 
GGCAAGCACA AGGGCTACGG CTTCATTGAG TACGAGAAGG CCCAGTCGTC CCAAGATGCT 
GTGTCTTCCA TGAACCTCTT TGACCTGGGT GGCCAGTACT TGCGGGTGGG CAAGGCTGTC 
ACACCGCCCA TGCCCCTACT CACACCAGCC ACGCCTGGAG GCCTCCCACC TGCCGCTGCT 
GTGGCAGCTG CTGCAGCCAC TGCCAAGATC ACAGCTCAGG AAGCAGTGGC CGGAGCAGCG 
GTGCTGGGTA CCCTGGGCAC ACCTGGACTG GTGTCCCCAG CACTGACCCT GGCCCAGCCC 
CTGGGCACTT TGCCCCAGGC TGTCATGGCT GCCCAGGCAC CTGGAGTCAT CACAGGTGTG 
ACCCCAGCCC GTCCTCCTAT CCCGGTCACC ATCCCCTCGG TGGGAGTGGT GAACCCCATC 
CTGGCCAGCC CTCCAACGCT GGGTCTCCTG GAGCCCAAGA AGGAGAAGGA AGAAGAGGAG 
CTGTTTCCCG AGTCAGAGCG GCCAGAGATG CTGAGCGAGC AGGAGCACAT GAGCATCTCG 
GGCAGTAGCG CCCGACACAT GGTGATGCAG AAGCTGCTCC GCAAGCAGGA GTCTACAGTG 
ATGGTTCTGC GCAACATGGT GGACCCCAAG GACATCGATG ATGACCTGGA AGGGGAGGTG 
ACAGAGGAGT GTGGCAAGTT CGGGGCCGTG AACCGCGTCA TCATCTACCA AGAGAAACAA 
GGCGAGGAGG AGGATGCAGA AATCATTGTC AAGATCTTTG TGGAGTTTTC CATAGCCTCT 
GAGACTCATA AGGCCATCCA GGCCCTCAAT GGCCGCTGGT TTGCTGGCCG CAAGGTGGTG 
GCTGAAGTGT ACGACCAGGA GCGTTTTGAT AACAGTGACC TCTCTGCGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016868727.1
CDS	34..1824
Translation	MEQLNLADSGLVGKEARRCRRASPPMGMPSAWWLQNEAGCLFAARQVNGQQGGGSEPAAAAAVVAAGDKWKPPQGTDSIKMENGQSTAAKLGLPPLTPEQQEALQKAKKYAMEQSIKSVLVKQTIAHQQQQLTNLQMAAVTMGFGDPLSPLQSMAAQRQRALAIMCRVYVGSIYYELGEDTIRQAFAPFGPIKSIDMSWDSVTMKHKGFAFVEYEVPEAAQLALEQMNSVMLGGRNIKVGRPSNIGQAQPIIDQLAEEARAFNRIYVASVHQDLSDDDIKSVFEAFGKIKSCTLARDPTTGKHKGYGFIEYEKAQSSQDAVSSMNLFDLGGQYLRVGKAVTPPMPLLTPATPGGLPPAAAVAAAAATAKITAQEAVAGAAVLGTLGTPGLVSPALTLAQPLGTLPQAVMAAQAPGVITGVTPARPPIPVTIPSVGVVNPILASPPTLGLLEPKKEKEEEELFPESERPEMLSEQEHMSISGSSARHMVMQKLLRKQESTVMVLRNMVDPKDIDDDLEGEVTEECGKFGAVNRVIIYQEKQGEEEDAEIIVKIFVEFSIASETHKAIQALNGRWFAGRKVVAEVYDQERFDNSDLSA

Target ORF information:

RefSeq Version	XM_017013238.1
Organism	Homo sapiens(human)
Definition	Homo sapiens poly(U) binding splicing factor 60KDa (PUF60), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017013238.1

ORF Insert Sequence:

ATGGAGCAGC TGAACTTGGC TGACAGTGGC CTGGTGGGGA AGGAAGCCAG AAGATGCAGA 
AGGGCCTCCC CTCCCATGGG CATGCCTTCT GCCTGGTGGC TGCAGAATGA GGCCGGCTGC 
CTCTTTGCAG CAAGGCAGGT CAATGGCCAG CAAGGAGGGG GGTCCGAGCC GGCGGCGGCG 
GCGGCAGTGG TGGCAGCGGG AGACAAATGG AAACCTCCAC AGGGCACAGA CTCCATCAAG 
ATGGAGAACG GGCAGAGCAC AGCCGCCAAG CTGGGGCTGC CTCCCCTGAC GCCCGAGCAG 
CAGGAGGCCC TTCAGAAGGC CAAGAAGTAC GCCATGGAGC AGAGCATCAA GAGTGTGCTG 
GTGAAGCAGA CCATCGCGCA CCAGCAGCAG CAGCTCACCA ACCTGCAGAT GGCAGCAGTG 
ACAATGGGCT TTGGAGATCC TCTCTCACCT TTGCAATCGA TGGCGGCTCA GCGGCAGCGG 
GCGCTGGCCA TCATGTGCCG CGTCTACGTG GGCTCTATCT ACTATGAGCT GGGGGAGGAC 
ACCATCCGCC AGGCCTTTGC CCCCTTTGGC CCCATCAAGA GCATCGACAT GTCCTGGGAC 
TCCGTCACCA TGAAGCACAA GGGCTTTGCC TTCGTGGAGT ATGAGGTCCC CGAAGCTGCA 
CAGCTGGCCT TGGAGCAGAT GAACTCGGTG ATGCTGGGGG GCAGGAACAT CAAGGTGGGC 
AGACCCAGCA ACATAGGGCA GGCCCAGCCC ATCATAGACC AGTTGGCTGA GGAGGCACGG 
GCCTTCAACC GCATCTACGT GGCCTCTGTG CACCAGGACC TCTCAGACGA TGACATCAAG 
AGCGTGTTTG AGGCCTTTGG CAAGATCAAG TCCTGCACAC TGGCCCGGGA CCCCACAACT 
GGCAAGCACA AGGGCTACGG CTTCATTGAG TACGAGAAGG CCCAGTCGTC CCAAGATGCT 
GTGTCTTCCA TGAACCTCTT TGACCTGGGT GGCCAGTACT TGCGGGTGGG CAAGGCTGTC 
ACACCGCCCA TGCCCCTACT CACACCAGCC ACGCCTGGAG GCCTCCCACC TGCCGCTGCT 
GTGGCAGCTG CTGCAGCCAC TGCCAAGATC ACAGCTCAGG AAGCAGTGGC CGGAGCAGCG 
GTGCTGGGTA CCCTGGGCAC ACCTGGACTG GTGTCCCCAG CACTGACCCT GGCCCAGCCC 
CTGGGCACTT TGCCCCAGGC TGTCATGGCT GCCCAGGCAC CTGGAGTCAT CACAGGTGTG 
ACCCCAGCCC GTCCTCCTAT CCCGGTCACC ATCCCCTCGG TGGGAGTGGT GAACCCCATC 
CTGGCCAGCC CTCCAACGCT GGGTCTCCTG GAGCCCAAGA AGGAGAAGGA AGAAGAGGAG 
CTGTTTCCCG AGTCAGAGCG GCCAGAGATG CTGAGCGAGC AGGAGCACAT GAGCATCTCG 
GGCAGTAGCG CCCGACACAT GGTGATGCAG AAGCTGCTCC GCAAGCAGGA GTCTACAGTG 
ATGGTTCTGC GCAACATGGT GGACCCCAAG GACATCGATG ATGACCTGGA AGGGGAGGTG 
ACAGAGGAGT GTGGCAAGTT CGGGGCCGTG AACCGCGTCA TCATCTACCA AGAGAAACAA 
GGCGAGGAGG AGGATGCAGA AATCATTGTC AAGATCTTTG TGGAGTTTTC CATAGCCTCT 
GAGACTCATA AGGCCATCCA GGCCCTCAAT GGCCGCTGGT TTGCTGGCCG CAAGGTGGTG 
GCTGAAGTGT ACGACCAGGA GCGTTTTGAT AACAGTGACC TCTCTGCGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PUF60 ( XM_017013237.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016868726.1 Homo sapiens poly(U) binding splicing factor 60KDa (PUF60), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OHu64963
Clone ID Related Accession (Same CDS sequence)	XM_011516929.2 , NM_001362896.2 , NM_001362895.1 , NM_001362896.1 , XM_017013238.1 , XM_017013237.1 , XM_017013234.1 , NM_001362895.2
Accession Version	XM_017013237.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1791bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	poly(U)-binding-splicing factor PUF60 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000008.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCAGC TGAACTTGGC TGACAGTGGC CTGGTGGGGA AGGAAGCCAG AAGATGCAGA 
AGGGCCTCCC CTCCCATGGG CATGCCTTCT GCCTGGTGGC TGCAGAATGA GGCCGGCTGC 
CTCTTTGCAG CAAGGCAGGT CAATGGCCAG CAAGGAGGGG GGTCCGAGCC GGCGGCGGCG 
GCGGCAGTGG TGGCAGCGGG AGACAAATGG AAACCTCCAC AGGGCACAGA CTCCATCAAG 
ATGGAGAACG GGCAGAGCAC AGCCGCCAAG CTGGGGCTGC CTCCCCTGAC GCCCGAGCAG 
CAGGAGGCCC TTCAGAAGGC CAAGAAGTAC GCCATGGAGC AGAGCATCAA GAGTGTGCTG 
GTGAAGCAGA CCATCGCGCA CCAGCAGCAG CAGCTCACCA ACCTGCAGAT GGCAGCAGTG 
ACAATGGGCT TTGGAGATCC TCTCTCACCT TTGCAATCGA TGGCGGCTCA GCGGCAGCGG 
GCGCTGGCCA TCATGTGCCG CGTCTACGTG GGCTCTATCT ACTATGAGCT GGGGGAGGAC 
ACCATCCGCC AGGCCTTTGC CCCCTTTGGC CCCATCAAGA GCATCGACAT GTCCTGGGAC 
TCCGTCACCA TGAAGCACAA GGGCTTTGCC TTCGTGGAGT ATGAGGTCCC CGAAGCTGCA 
CAGCTGGCCT TGGAGCAGAT GAACTCGGTG ATGCTGGGGG GCAGGAACAT CAAGGTGGGC 
AGACCCAGCA ACATAGGGCA GGCCCAGCCC ATCATAGACC AGTTGGCTGA GGAGGCACGG 
GCCTTCAACC GCATCTACGT GGCCTCTGTG CACCAGGACC TCTCAGACGA TGACATCAAG 
AGCGTGTTTG AGGCCTTTGG CAAGATCAAG TCCTGCACAC TGGCCCGGGA CCCCACAACT 
GGCAAGCACA AGGGCTACGG CTTCATTGAG TACGAGAAGG CCCAGTCGTC CCAAGATGCT 
GTGTCTTCCA TGAACCTCTT TGACCTGGGT GGCCAGTACT TGCGGGTGGG CAAGGCTGTC 
ACACCGCCCA TGCCCCTACT CACACCAGCC ACGCCTGGAG GCCTCCCACC TGCCGCTGCT 
GTGGCAGCTG CTGCAGCCAC TGCCAAGATC ACAGCTCAGG AAGCAGTGGC CGGAGCAGCG 
GTGCTGGGTA CCCTGGGCAC ACCTGGACTG GTGTCCCCAG CACTGACCCT GGCCCAGCCC 
CTGGGCACTT TGCCCCAGGC TGTCATGGCT GCCCAGGCAC CTGGAGTCAT CACAGGTGTG 
ACCCCAGCCC GTCCTCCTAT CCCGGTCACC ATCCCCTCGG TGGGAGTGGT GAACCCCATC 
CTGGCCAGCC CTCCAACGCT GGGTCTCCTG GAGCCCAAGA AGGAGAAGGA AGAAGAGGAG 
CTGTTTCCCG AGTCAGAGCG GCCAGAGATG CTGAGCGAGC AGGAGCACAT GAGCATCTCG 
GGCAGTAGCG CCCGACACAT GGTGATGCAG AAGCTGCTCC GCAAGCAGGA GTCTACAGTG 
ATGGTTCTGC GCAACATGGT GGACCCCAAG GACATCGATG ATGACCTGGA AGGGGAGGTG 
ACAGAGGAGT GTGGCAAGTT CGGGGCCGTG AACCGCGTCA TCATCTACCA AGAGAAACAA 
GGCGAGGAGG AGGATGCAGA AATCATTGTC AAGATCTTTG TGGAGTTTTC CATAGCCTCT 
GAGACTCATA AGGCCATCCA GGCCCTCAAT GGCCGCTGGT TTGCTGGCCG CAAGGTGGTG 
GCTGAAGTGT ACGACCAGGA GCGTTTTGAT AACAGTGACC TCTCTGCGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016868726.1
CDS	234..2024
Translation	MEQLNLADSGLVGKEARRCRRASPPMGMPSAWWLQNEAGCLFAARQVNGQQGGGSEPAAAAAVVAAGDKWKPPQGTDSIKMENGQSTAAKLGLPPLTPEQQEALQKAKKYAMEQSIKSVLVKQTIAHQQQQLTNLQMAAVTMGFGDPLSPLQSMAAQRQRALAIMCRVYVGSIYYELGEDTIRQAFAPFGPIKSIDMSWDSVTMKHKGFAFVEYEVPEAAQLALEQMNSVMLGGRNIKVGRPSNIGQAQPIIDQLAEEARAFNRIYVASVHQDLSDDDIKSVFEAFGKIKSCTLARDPTTGKHKGYGFIEYEKAQSSQDAVSSMNLFDLGGQYLRVGKAVTPPMPLLTPATPGGLPPAAAVAAAAATAKITAQEAVAGAAVLGTLGTPGLVSPALTLAQPLGTLPQAVMAAQAPGVITGVTPARPPIPVTIPSVGVVNPILASPPTLGLLEPKKEKEEEELFPESERPEMLSEQEHMSISGSSARHMVMQKLLRKQESTVMVLRNMVDPKDIDDDLEGEVTEECGKFGAVNRVIIYQEKQGEEEDAEIIVKIFVEFSIASETHKAIQALNGRWFAGRKVVAEVYDQERFDNSDLSA

Target ORF information:

RefSeq Version	XM_017013237.1
Organism	Homo sapiens(human)
Definition	Homo sapiens poly(U) binding splicing factor 60KDa (PUF60), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017013237.1

ORF Insert Sequence:

ATGGAGCAGC TGAACTTGGC TGACAGTGGC CTGGTGGGGA AGGAAGCCAG AAGATGCAGA 
AGGGCCTCCC CTCCCATGGG CATGCCTTCT GCCTGGTGGC TGCAGAATGA GGCCGGCTGC 
CTCTTTGCAG CAAGGCAGGT CAATGGCCAG CAAGGAGGGG GGTCCGAGCC GGCGGCGGCG 
GCGGCAGTGG TGGCAGCGGG AGACAAATGG AAACCTCCAC AGGGCACAGA CTCCATCAAG 
ATGGAGAACG GGCAGAGCAC AGCCGCCAAG CTGGGGCTGC CTCCCCTGAC GCCCGAGCAG 
CAGGAGGCCC TTCAGAAGGC CAAGAAGTAC GCCATGGAGC AGAGCATCAA GAGTGTGCTG 
GTGAAGCAGA CCATCGCGCA CCAGCAGCAG CAGCTCACCA ACCTGCAGAT GGCAGCAGTG 
ACAATGGGCT TTGGAGATCC TCTCTCACCT TTGCAATCGA TGGCGGCTCA GCGGCAGCGG 
GCGCTGGCCA TCATGTGCCG CGTCTACGTG GGCTCTATCT ACTATGAGCT GGGGGAGGAC 
ACCATCCGCC AGGCCTTTGC CCCCTTTGGC CCCATCAAGA GCATCGACAT GTCCTGGGAC 
TCCGTCACCA TGAAGCACAA GGGCTTTGCC TTCGTGGAGT ATGAGGTCCC CGAAGCTGCA 
CAGCTGGCCT TGGAGCAGAT GAACTCGGTG ATGCTGGGGG GCAGGAACAT CAAGGTGGGC 
AGACCCAGCA ACATAGGGCA GGCCCAGCCC ATCATAGACC AGTTGGCTGA GGAGGCACGG 
GCCTTCAACC GCATCTACGT GGCCTCTGTG CACCAGGACC TCTCAGACGA TGACATCAAG 
AGCGTGTTTG AGGCCTTTGG CAAGATCAAG TCCTGCACAC TGGCCCGGGA CCCCACAACT 
GGCAAGCACA AGGGCTACGG CTTCATTGAG TACGAGAAGG CCCAGTCGTC CCAAGATGCT 
GTGTCTTCCA TGAACCTCTT TGACCTGGGT GGCCAGTACT TGCGGGTGGG CAAGGCTGTC 
ACACCGCCCA TGCCCCTACT CACACCAGCC ACGCCTGGAG GCCTCCCACC TGCCGCTGCT 
GTGGCAGCTG CTGCAGCCAC TGCCAAGATC ACAGCTCAGG AAGCAGTGGC CGGAGCAGCG 
GTGCTGGGTA CCCTGGGCAC ACCTGGACTG GTGTCCCCAG CACTGACCCT GGCCCAGCCC 
CTGGGCACTT TGCCCCAGGC TGTCATGGCT GCCCAGGCAC CTGGAGTCAT CACAGGTGTG 
ACCCCAGCCC GTCCTCCTAT CCCGGTCACC ATCCCCTCGG TGGGAGTGGT GAACCCCATC 
CTGGCCAGCC CTCCAACGCT GGGTCTCCTG GAGCCCAAGA AGGAGAAGGA AGAAGAGGAG 
CTGTTTCCCG AGTCAGAGCG GCCAGAGATG CTGAGCGAGC AGGAGCACAT GAGCATCTCG 
GGCAGTAGCG CCCGACACAT GGTGATGCAG AAGCTGCTCC GCAAGCAGGA GTCTACAGTG 
ATGGTTCTGC GCAACATGGT GGACCCCAAG GACATCGATG ATGACCTGGA AGGGGAGGTG 
ACAGAGGAGT GTGGCAAGTT CGGGGCCGTG AACCGCGTCA TCATCTACCA AGAGAAACAA 
GGCGAGGAGG AGGATGCAGA AATCATTGTC AAGATCTTTG TGGAGTTTTC CATAGCCTCT 
GAGACTCATA AGGCCATCCA GGCCCTCAAT GGCCGCTGGT TTGCTGGCCG CAAGGTGGTG 
GCTGAAGTGT ACGACCAGGA GCGTTTTGAT AACAGTGACC TCTCTGCGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PUF60 ( XM_017013234.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016868723.1 Homo sapiens poly(U) binding splicing factor 60 (PUF60), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OHu64963
Clone ID Related Accession (Same CDS sequence)	XM_011516929.2 , NM_001362896.2 , NM_001362895.1 , NM_001362896.1 , XM_017013238.1 , XM_017013237.1 , XM_017013234.1 , NM_001362895.2
Accession Version	XM_017013234.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1791bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	poly(U)-binding-splicing factor PUF60 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000008.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCAGC TGAACTTGGC TGACAGTGGC CTGGTGGGGA AGGAAGCCAG AAGATGCAGA 
AGGGCCTCCC CTCCCATGGG CATGCCTTCT GCCTGGTGGC TGCAGAATGA GGCCGGCTGC 
CTCTTTGCAG CAAGGCAGGT CAATGGCCAG CAAGGAGGGG GGTCCGAGCC GGCGGCGGCG 
GCGGCAGTGG TGGCAGCGGG AGACAAATGG AAACCTCCAC AGGGCACAGA CTCCATCAAG 
ATGGAGAACG GGCAGAGCAC AGCCGCCAAG CTGGGGCTGC CTCCCCTGAC GCCCGAGCAG 
CAGGAGGCCC TTCAGAAGGC CAAGAAGTAC GCCATGGAGC AGAGCATCAA GAGTGTGCTG 
GTGAAGCAGA CCATCGCGCA CCAGCAGCAG CAGCTCACCA ACCTGCAGAT GGCAGCAGTG 
ACAATGGGCT TTGGAGATCC TCTCTCACCT TTGCAATCGA TGGCGGCTCA GCGGCAGCGG 
GCGCTGGCCA TCATGTGCCG CGTCTACGTG GGCTCTATCT ACTATGAGCT GGGGGAGGAC 
ACCATCCGCC AGGCCTTTGC CCCCTTTGGC CCCATCAAGA GCATCGACAT GTCCTGGGAC 
TCCGTCACCA TGAAGCACAA GGGCTTTGCC TTCGTGGAGT ATGAGGTCCC CGAAGCTGCA 
CAGCTGGCCT TGGAGCAGAT GAACTCGGTG ATGCTGGGGG GCAGGAACAT CAAGGTGGGC 
AGACCCAGCA ACATAGGGCA GGCCCAGCCC ATCATAGACC AGTTGGCTGA GGAGGCACGG 
GCCTTCAACC GCATCTACGT GGCCTCTGTG CACCAGGACC TCTCAGACGA TGACATCAAG 
AGCGTGTTTG AGGCCTTTGG CAAGATCAAG TCCTGCACAC TGGCCCGGGA CCCCACAACT 
GGCAAGCACA AGGGCTACGG CTTCATTGAG TACGAGAAGG CCCAGTCGTC CCAAGATGCT 
GTGTCTTCCA TGAACCTCTT TGACCTGGGT GGCCAGTACT TGCGGGTGGG CAAGGCTGTC 
ACACCGCCCA TGCCCCTACT CACACCAGCC ACGCCTGGAG GCCTCCCACC TGCCGCTGCT 
GTGGCAGCTG CTGCAGCCAC TGCCAAGATC ACAGCTCAGG AAGCAGTGGC CGGAGCAGCG 
GTGCTGGGTA CCCTGGGCAC ACCTGGACTG GTGTCCCCAG CACTGACCCT GGCCCAGCCC 
CTGGGCACTT TGCCCCAGGC TGTCATGGCT GCCCAGGCAC CTGGAGTCAT CACAGGTGTG 
ACCCCAGCCC GTCCTCCTAT CCCGGTCACC ATCCCCTCGG TGGGAGTGGT GAACCCCATC 
CTGGCCAGCC CTCCAACGCT GGGTCTCCTG GAGCCCAAGA AGGAGAAGGA AGAAGAGGAG 
CTGTTTCCCG AGTCAGAGCG GCCAGAGATG CTGAGCGAGC AGGAGCACAT GAGCATCTCG 
GGCAGTAGCG CCCGACACAT GGTGATGCAG AAGCTGCTCC GCAAGCAGGA GTCTACAGTG 
ATGGTTCTGC GCAACATGGT GGACCCCAAG GACATCGATG ATGACCTGGA AGGGGAGGTG 
ACAGAGGAGT GTGGCAAGTT CGGGGCCGTG AACCGCGTCA TCATCTACCA AGAGAAACAA 
GGCGAGGAGG AGGATGCAGA AATCATTGTC AAGATCTTTG TGGAGTTTTC CATAGCCTCT 
GAGACTCATA AGGCCATCCA GGCCCTCAAT GGCCGCTGGT TTGCTGGCCG CAAGGTGGTG 
GCTGAAGTGT ACGACCAGGA GCGTTTTGAT AACAGTGACC TCTCTGCGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016868723.1
CDS	1505..3295
Translation	MEQLNLADSGLVGKEARRCRRASPPMGMPSAWWLQNEAGCLFAARQVNGQQGGGSEPAAAAAVVAAGDKWKPPQGTDSIKMENGQSTAAKLGLPPLTPEQQEALQKAKKYAMEQSIKSVLVKQTIAHQQQQLTNLQMAAVTMGFGDPLSPLQSMAAQRQRALAIMCRVYVGSIYYELGEDTIRQAFAPFGPIKSIDMSWDSVTMKHKGFAFVEYEVPEAAQLALEQMNSVMLGGRNIKVGRPSNIGQAQPIIDQLAEEARAFNRIYVASVHQDLSDDDIKSVFEAFGKIKSCTLARDPTTGKHKGYGFIEYEKAQSSQDAVSSMNLFDLGGQYLRVGKAVTPPMPLLTPATPGGLPPAAAVAAAAATAKITAQEAVAGAAVLGTLGTPGLVSPALTLAQPLGTLPQAVMAAQAPGVITGVTPARPPIPVTIPSVGVVNPILASPPTLGLLEPKKEKEEEELFPESERPEMLSEQEHMSISGSSARHMVMQKLLRKQESTVMVLRNMVDPKDIDDDLEGEVTEECGKFGAVNRVIIYQEKQGEEEDAEIIVKIFVEFSIASETHKAIQALNGRWFAGRKVVAEVYDQERFDNSDLSA

Target ORF information:

RefSeq Version	XM_017013234.1
Organism	Homo sapiens(human)
Definition	Homo sapiens poly(U) binding splicing factor 60 (PUF60), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017013234.1

ORF Insert Sequence:

ATGGAGCAGC TGAACTTGGC TGACAGTGGC CTGGTGGGGA AGGAAGCCAG AAGATGCAGA 
AGGGCCTCCC CTCCCATGGG CATGCCTTCT GCCTGGTGGC TGCAGAATGA GGCCGGCTGC 
CTCTTTGCAG CAAGGCAGGT CAATGGCCAG CAAGGAGGGG GGTCCGAGCC GGCGGCGGCG 
GCGGCAGTGG TGGCAGCGGG AGACAAATGG AAACCTCCAC AGGGCACAGA CTCCATCAAG 
ATGGAGAACG GGCAGAGCAC AGCCGCCAAG CTGGGGCTGC CTCCCCTGAC GCCCGAGCAG 
CAGGAGGCCC TTCAGAAGGC CAAGAAGTAC GCCATGGAGC AGAGCATCAA GAGTGTGCTG 
GTGAAGCAGA CCATCGCGCA CCAGCAGCAG CAGCTCACCA ACCTGCAGAT GGCAGCAGTG 
ACAATGGGCT TTGGAGATCC TCTCTCACCT TTGCAATCGA TGGCGGCTCA GCGGCAGCGG 
GCGCTGGCCA TCATGTGCCG CGTCTACGTG GGCTCTATCT ACTATGAGCT GGGGGAGGAC 
ACCATCCGCC AGGCCTTTGC CCCCTTTGGC CCCATCAAGA GCATCGACAT GTCCTGGGAC 
TCCGTCACCA TGAAGCACAA GGGCTTTGCC TTCGTGGAGT ATGAGGTCCC CGAAGCTGCA 
CAGCTGGCCT TGGAGCAGAT GAACTCGGTG ATGCTGGGGG GCAGGAACAT CAAGGTGGGC 
AGACCCAGCA ACATAGGGCA GGCCCAGCCC ATCATAGACC AGTTGGCTGA GGAGGCACGG 
GCCTTCAACC GCATCTACGT GGCCTCTGTG CACCAGGACC TCTCAGACGA TGACATCAAG 
AGCGTGTTTG AGGCCTTTGG CAAGATCAAG TCCTGCACAC TGGCCCGGGA CCCCACAACT 
GGCAAGCACA AGGGCTACGG CTTCATTGAG TACGAGAAGG CCCAGTCGTC CCAAGATGCT 
GTGTCTTCCA TGAACCTCTT TGACCTGGGT GGCCAGTACT TGCGGGTGGG CAAGGCTGTC 
ACACCGCCCA TGCCCCTACT CACACCAGCC ACGCCTGGAG GCCTCCCACC TGCCGCTGCT 
GTGGCAGCTG CTGCAGCCAC TGCCAAGATC ACAGCTCAGG AAGCAGTGGC CGGAGCAGCG 
GTGCTGGGTA CCCTGGGCAC ACCTGGACTG GTGTCCCCAG CACTGACCCT GGCCCAGCCC 
CTGGGCACTT TGCCCCAGGC TGTCATGGCT GCCCAGGCAC CTGGAGTCAT CACAGGTGTG 
ACCCCAGCCC GTCCTCCTAT CCCGGTCACC ATCCCCTCGG TGGGAGTGGT GAACCCCATC 
CTGGCCAGCC CTCCAACGCT GGGTCTCCTG GAGCCCAAGA AGGAGAAGGA AGAAGAGGAG 
CTGTTTCCCG AGTCAGAGCG GCCAGAGATG CTGAGCGAGC AGGAGCACAT GAGCATCTCG 
GGCAGTAGCG CCCGACACAT GGTGATGCAG AAGCTGCTCC GCAAGCAGGA GTCTACAGTG 
ATGGTTCTGC GCAACATGGT GGACCCCAAG GACATCGATG ATGACCTGGA AGGGGAGGTG 
ACAGAGGAGT GTGGCAAGTT CGGGGCCGTG AACCGCGTCA TCATCTACCA AGAGAAACAA 
GGCGAGGAGG AGGATGCAGA AATCATTGTC AAGATCTTTG TGGAGTTTTC CATAGCCTCT 
GAGACTCATA AGGCCATCCA GGCCCTCAAT GGCCGCTGGT TTGCTGGCCG CAAGGTGGTG 
GCTGAAGTGT ACGACCAGGA GCGTTTTGAT AACAGTGACC TCTCTGCGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PUF60 ( XM_017013236.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016868725.1 Homo sapiens poly(U) binding splicing factor 60 (PUF60), transcript variant X10, mRNA.

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CloneID	OHu64964
Clone ID Related Accession (Same CDS sequence)	NM_001362897.1 , XM_017013236.1 , NM_001362897.2 , XM_011516930.2
Accession Version	XM_017013236.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1740bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	poly(U)-binding-splicing factor PUF60 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000008.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCAGC TGAACTTGGC TGACAGTGGC CTGGTGGGGA AGGAAGCCAG AAGATGCAGA 
AGGGCCTCCC CTCCCATGGG CATGCCTTCT GCCTGGTGGC TGCAGAATGA GGCCGGCTGC 
CTCTTTGCAG CAAGGCAGGT CAATGGCCAG CAAGGAGGGG GGTCCGAGCC GGCGGCGGCG 
GCGGCAGTGG TGGCAGCGGG AGACAAATGG AAACCTCCAC AGGGCACAGA CTCCATCAAG 
ATGGAGAACG GGCAGAGCAC AGCCGCCAAG CTGGGGCTGC CTCCCCTGAC GCCCGAGCAG 
CAGGAGGCCC TTCAGAAGGC CAAGAAGTAC GCCATGGAGC AGAGCATCAA GAGTGTGCTG 
GTGAAGCAGA CCATCGCGCA CCAGCAGCAG CAGCTCACCA ACCTGCAGAT GGCGGCTCAG 
CGGCAGCGGG CGCTGGCCAT CATGTGCCGC GTCTACGTGG GCTCTATCTA CTATGAGCTG 
GGGGAGGACA CCATCCGCCA GGCCTTTGCC CCCTTTGGCC CCATCAAGAG CATCGACATG 
TCCTGGGACT CCGTCACCAT GAAGCACAAG GGCTTTGCCT TCGTGGAGTA TGAGGTCCCC 
GAAGCTGCAC AGCTGGCCTT GGAGCAGATG AACTCGGTGA TGCTGGGGGG CAGGAACATC 
AAGGTGGGCA GACCCAGCAA CATAGGGCAG GCCCAGCCCA TCATAGACCA GTTGGCTGAG 
GAGGCACGGG CCTTCAACCG CATCTACGTG GCCTCTGTGC ACCAGGACCT CTCAGACGAT 
GACATCAAGA GCGTGTTTGA GGCCTTTGGC AAGATCAAGT CCTGCACACT GGCCCGGGAC 
CCCACAACTG GCAAGCACAA GGGCTACGGC TTCATTGAGT ACGAGAAGGC CCAGTCGTCC 
CAAGATGCTG TGTCTTCCAT GAACCTCTTT GACCTGGGTG GCCAGTACTT GCGGGTGGGC 
AAGGCTGTCA CACCGCCCAT GCCCCTACTC ACACCAGCCA CGCCTGGAGG CCTCCCACCT 
GCCGCTGCTG TGGCAGCTGC TGCAGCCACT GCCAAGATCA CAGCTCAGGA AGCAGTGGCC 
GGAGCAGCGG TGCTGGGTAC CCTGGGCACA CCTGGACTGG TGTCCCCAGC ACTGACCCTG 
GCCCAGCCCC TGGGCACTTT GCCCCAGGCT GTCATGGCTG CCCAGGCACC TGGAGTCATC 
ACAGGTGTGA CCCCAGCCCG TCCTCCTATC CCGGTCACCA TCCCCTCGGT GGGAGTGGTG 
AACCCCATCC TGGCCAGCCC TCCAACGCTG GGTCTCCTGG AGCCCAAGAA GGAGAAGGAA 
GAAGAGGAGC TGTTTCCCGA GTCAGAGCGG CCAGAGATGC TGAGCGAGCA GGAGCACATG 
AGCATCTCGG GCAGTAGCGC CCGACACATG GTGATGCAGA AGCTGCTCCG CAAGCAGGAG 
TCTACAGTGA TGGTTCTGCG CAACATGGTG GACCCCAAGG ACATCGATGA TGACCTGGAA 
GGGGAGGTGA CAGAGGAGTG TGGCAAGTTC GGGGCCGTGA ACCGCGTCAT CATCTACCAA 
GAGAAACAAG GCGAGGAGGA GGATGCAGAA ATCATTGTCA AGATCTTTGT GGAGTTTTCC 
ATAGCCTCTG AGACTCATAA GGCCATCCAG GCCCTCAATG GCCGCTGGTT TGCTGGCCGC 
AAGGTGGTGG CTGAAGTGTA CGACCAGGAG CGTTTTGATA ACAGTGACCT CTCTGCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016868725.1
CDS	1505..3244
Translation	MEQLNLADSGLVGKEARRCRRASPPMGMPSAWWLQNEAGCLFAARQVNGQQGGGSEPAAAAAVVAAGDKWKPPQGTDSIKMENGQSTAAKLGLPPLTPEQQEALQKAKKYAMEQSIKSVLVKQTIAHQQQQLTNLQMAAQRQRALAIMCRVYVGSIYYELGEDTIRQAFAPFGPIKSIDMSWDSVTMKHKGFAFVEYEVPEAAQLALEQMNSVMLGGRNIKVGRPSNIGQAQPIIDQLAEEARAFNRIYVASVHQDLSDDDIKSVFEAFGKIKSCTLARDPTTGKHKGYGFIEYEKAQSSQDAVSSMNLFDLGGQYLRVGKAVTPPMPLLTPATPGGLPPAAAVAAAAATAKITAQEAVAGAAVLGTLGTPGLVSPALTLAQPLGTLPQAVMAAQAPGVITGVTPARPPIPVTIPSVGVVNPILASPPTLGLLEPKKEKEEEELFPESERPEMLSEQEHMSISGSSARHMVMQKLLRKQESTVMVLRNMVDPKDIDDDLEGEVTEECGKFGAVNRVIIYQEKQGEEEDAEIIVKIFVEFSIASETHKAIQALNGRWFAGRKVVAEVYDQERFDNSDLSA

Target ORF information:

RefSeq Version	XM_017013236.1
Organism	Homo sapiens(human)
Definition	Homo sapiens poly(U) binding splicing factor 60 (PUF60), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017013236.1

ORF Insert Sequence:

ATGGAGCAGC TGAACTTGGC TGACAGTGGC CTGGTGGGGA AGGAAGCCAG AAGATGCAGA 
AGGGCCTCCC CTCCCATGGG CATGCCTTCT GCCTGGTGGC TGCAGAATGA GGCCGGCTGC 
CTCTTTGCAG CAAGGCAGGT CAATGGCCAG CAAGGAGGGG GGTCCGAGCC GGCGGCGGCG 
GCGGCAGTGG TGGCAGCGGG AGACAAATGG AAACCTCCAC AGGGCACAGA CTCCATCAAG 
ATGGAGAACG GGCAGAGCAC AGCCGCCAAG CTGGGGCTGC CTCCCCTGAC GCCCGAGCAG 
CAGGAGGCCC TTCAGAAGGC CAAGAAGTAC GCCATGGAGC AGAGCATCAA GAGTGTGCTG 
GTGAAGCAGA CCATCGCGCA CCAGCAGCAG CAGCTCACCA ACCTGCAGAT GGCGGCTCAG 
CGGCAGCGGG CGCTGGCCAT CATGTGCCGC GTCTACGTGG GCTCTATCTA CTATGAGCTG 
GGGGAGGACA CCATCCGCCA GGCCTTTGCC CCCTTTGGCC CCATCAAGAG CATCGACATG 
TCCTGGGACT CCGTCACCAT GAAGCACAAG GGCTTTGCCT TCGTGGAGTA TGAGGTCCCC 
GAAGCTGCAC AGCTGGCCTT GGAGCAGATG AACTCGGTGA TGCTGGGGGG CAGGAACATC 
AAGGTGGGCA GACCCAGCAA CATAGGGCAG GCCCAGCCCA TCATAGACCA GTTGGCTGAG 
GAGGCACGGG CCTTCAACCG CATCTACGTG GCCTCTGTGC ACCAGGACCT CTCAGACGAT 
GACATCAAGA GCGTGTTTGA GGCCTTTGGC AAGATCAAGT CCTGCACACT GGCCCGGGAC 
CCCACAACTG GCAAGCACAA GGGCTACGGC TTCATTGAGT ACGAGAAGGC CCAGTCGTCC 
CAAGATGCTG TGTCTTCCAT GAACCTCTTT GACCTGGGTG GCCAGTACTT GCGGGTGGGC 
AAGGCTGTCA CACCGCCCAT GCCCCTACTC ACACCAGCCA CGCCTGGAGG CCTCCCACCT 
GCCGCTGCTG TGGCAGCTGC TGCAGCCACT GCCAAGATCA CAGCTCAGGA AGCAGTGGCC 
GGAGCAGCGG TGCTGGGTAC CCTGGGCACA CCTGGACTGG TGTCCCCAGC ACTGACCCTG 
GCCCAGCCCC TGGGCACTTT GCCCCAGGCT GTCATGGCTG CCCAGGCACC TGGAGTCATC 
ACAGGTGTGA CCCCAGCCCG TCCTCCTATC CCGGTCACCA TCCCCTCGGT GGGAGTGGTG 
AACCCCATCC TGGCCAGCCC TCCAACGCTG GGTCTCCTGG AGCCCAAGAA GGAGAAGGAA 
GAAGAGGAGC TGTTTCCCGA GTCAGAGCGG CCAGAGATGC TGAGCGAGCA GGAGCACATG 
AGCATCTCGG GCAGTAGCGC CCGACACATG GTGATGCAGA AGCTGCTCCG CAAGCAGGAG 
TCTACAGTGA TGGTTCTGCG CAACATGGTG GACCCCAAGG ACATCGATGA TGACCTGGAA 
GGGGAGGTGA CAGAGGAGTG TGGCAAGTTC GGGGCCGTGA ACCGCGTCAT CATCTACCAA 
GAGAAACAAG GCGAGGAGGA GGATGCAGAA ATCATTGTCA AGATCTTTGT GGAGTTTTCC 
ATAGCCTCTG AGACTCATAA GGCCATCCAG GCCCTCAATG GCCGCTGGTT TGCTGGCCGC 
AAGGTGGTGG CTGAAGTGTA CGACCAGGAG CGTTTTGATA ACAGTGACCT CTCTGCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PUF60 ( XM_011516930.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011515232.1 Homo sapiens poly(U) binding splicing factor 60KDa (PUF60), transcript variant X5, mRNA.

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CloneID	OHu64964
Clone ID Related Accession (Same CDS sequence)	NM_001362897.1 , XM_017013236.1 , NM_001362897.2 , XM_011516930.2
Accession Version	XM_011516930.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1740bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	poly(U)-binding-splicing factor PUF60 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000008.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011516930.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCAGC TGAACTTGGC TGACAGTGGC CTGGTGGGGA AGGAAGCCAG AAGATGCAGA 
AGGGCCTCCC CTCCCATGGG CATGCCTTCT GCCTGGTGGC TGCAGAATGA GGCCGGCTGC 
CTCTTTGCAG CAAGGCAGGT CAATGGCCAG CAAGGAGGGG GGTCCGAGCC GGCGGCGGCG 
GCGGCAGTGG TGGCAGCGGG AGACAAATGG AAACCTCCAC AGGGCACAGA CTCCATCAAG 
ATGGAGAACG GGCAGAGCAC AGCCGCCAAG CTGGGGCTGC CTCCCCTGAC GCCCGAGCAG 
CAGGAGGCCC TTCAGAAGGC CAAGAAGTAC GCCATGGAGC AGAGCATCAA GAGTGTGCTG 
GTGAAGCAGA CCATCGCGCA CCAGCAGCAG CAGCTCACCA ACCTGCAGAT GGCGGCTCAG 
CGGCAGCGGG CGCTGGCCAT CATGTGCCGC GTCTACGTGG GCTCTATCTA CTATGAGCTG 
GGGGAGGACA CCATCCGCCA GGCCTTTGCC CCCTTTGGCC CCATCAAGAG CATCGACATG 
TCCTGGGACT CCGTCACCAT GAAGCACAAG GGCTTTGCCT TCGTGGAGTA TGAGGTCCCC 
GAAGCTGCAC AGCTGGCCTT GGAGCAGATG AACTCGGTGA TGCTGGGGGG CAGGAACATC 
AAGGTGGGCA GACCCAGCAA CATAGGGCAG GCCCAGCCCA TCATAGACCA GTTGGCTGAG 
GAGGCACGGG CCTTCAACCG CATCTACGTG GCCTCTGTGC ACCAGGACCT CTCAGACGAT 
GACATCAAGA GCGTGTTTGA GGCCTTTGGC AAGATCAAGT CCTGCACACT GGCCCGGGAC 
CCCACAACTG GCAAGCACAA GGGCTACGGC TTCATTGAGT ACGAGAAGGC CCAGTCGTCC 
CAAGATGCTG TGTCTTCCAT GAACCTCTTT GACCTGGGTG GCCAGTACTT GCGGGTGGGC 
AAGGCTGTCA CACCGCCCAT GCCCCTACTC ACACCAGCCA CGCCTGGAGG CCTCCCACCT 
GCCGCTGCTG TGGCAGCTGC TGCAGCCACT GCCAAGATCA CAGCTCAGGA AGCAGTGGCC 
GGAGCAGCGG TGCTGGGTAC CCTGGGCACA CCTGGACTGG TGTCCCCAGC ACTGACCCTG 
GCCCAGCCCC TGGGCACTTT GCCCCAGGCT GTCATGGCTG CCCAGGCACC TGGAGTCATC 
ACAGGTGTGA CCCCAGCCCG TCCTCCTATC CCGGTCACCA TCCCCTCGGT GGGAGTGGTG 
AACCCCATCC TGGCCAGCCC TCCAACGCTG GGTCTCCTGG AGCCCAAGAA GGAGAAGGAA 
GAAGAGGAGC TGTTTCCCGA GTCAGAGCGG CCAGAGATGC TGAGCGAGCA GGAGCACATG 
AGCATCTCGG GCAGTAGCGC CCGACACATG GTGATGCAGA AGCTGCTCCG CAAGCAGGAG 
TCTACAGTGA TGGTTCTGCG CAACATGGTG GACCCCAAGG ACATCGATGA TGACCTGGAA 
GGGGAGGTGA CAGAGGAGTG TGGCAAGTTC GGGGCCGTGA ACCGCGTCAT CATCTACCAA 
GAGAAACAAG GCGAGGAGGA GGATGCAGAA ATCATTGTCA AGATCTTTGT GGAGTTTTCC 
ATAGCCTCTG AGACTCATAA GGCCATCCAG GCCCTCAATG GCCGCTGGTT TGCTGGCCGC 
AAGGTGGTGG CTGAAGTGTA CGACCAGGAG CGTTTTGATA ACAGTGACCT CTCTGCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011515232.1
CDS	74..1813
Translation	MEQLNLADSGLVGKEARRCRRASPPMGMPSAWWLQNEAGCLFAARQVNGQQGGGSEPAAAAAVVAAGDKWKPPQGTDSIKMENGQSTAAKLGLPPLTPEQQEALQKAKKYAMEQSIKSVLVKQTIAHQQQQLTNLQMAAQRQRALAIMCRVYVGSIYYELGEDTIRQAFAPFGPIKSIDMSWDSVTMKHKGFAFVEYEVPEAAQLALEQMNSVMLGGRNIKVGRPSNIGQAQPIIDQLAEEARAFNRIYVASVHQDLSDDDIKSVFEAFGKIKSCTLARDPTTGKHKGYGFIEYEKAQSSQDAVSSMNLFDLGGQYLRVGKAVTPPMPLLTPATPGGLPPAAAVAAAAATAKITAQEAVAGAAVLGTLGTPGLVSPALTLAQPLGTLPQAVMAAQAPGVITGVTPARPPIPVTIPSVGVVNPILASPPTLGLLEPKKEKEEEELFPESERPEMLSEQEHMSISGSSARHMVMQKLLRKQESTVMVLRNMVDPKDIDDDLEGEVTEECGKFGAVNRVIIYQEKQGEEEDAEIIVKIFVEFSIASETHKAIQALNGRWFAGRKVVAEVYDQERFDNSDLSA

Target ORF information:

RefSeq Version	XM_011516930.2
Organism	Homo sapiens(human)
Definition	Homo sapiens poly(U) binding splicing factor 60KDa (PUF60), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011516930.2

ORF Insert Sequence:

ATGGAGCAGC TGAACTTGGC TGACAGTGGC CTGGTGGGGA AGGAAGCCAG AAGATGCAGA 
AGGGCCTCCC CTCCCATGGG CATGCCTTCT GCCTGGTGGC TGCAGAATGA GGCCGGCTGC 
CTCTTTGCAG CAAGGCAGGT CAATGGCCAG CAAGGAGGGG GGTCCGAGCC GGCGGCGGCG 
GCGGCAGTGG TGGCAGCGGG AGACAAATGG AAACCTCCAC AGGGCACAGA CTCCATCAAG 
ATGGAGAACG GGCAGAGCAC AGCCGCCAAG CTGGGGCTGC CTCCCCTGAC GCCCGAGCAG 
CAGGAGGCCC TTCAGAAGGC CAAGAAGTAC GCCATGGAGC AGAGCATCAA GAGTGTGCTG 
GTGAAGCAGA CCATCGCGCA CCAGCAGCAG CAGCTCACCA ACCTGCAGAT GGCGGCTCAG 
CGGCAGCGGG CGCTGGCCAT CATGTGCCGC GTCTACGTGG GCTCTATCTA CTATGAGCTG 
GGGGAGGACA CCATCCGCCA GGCCTTTGCC CCCTTTGGCC CCATCAAGAG CATCGACATG 
TCCTGGGACT CCGTCACCAT GAAGCACAAG GGCTTTGCCT TCGTGGAGTA TGAGGTCCCC 
GAAGCTGCAC AGCTGGCCTT GGAGCAGATG AACTCGGTGA TGCTGGGGGG CAGGAACATC 
AAGGTGGGCA GACCCAGCAA CATAGGGCAG GCCCAGCCCA TCATAGACCA GTTGGCTGAG 
GAGGCACGGG CCTTCAACCG CATCTACGTG GCCTCTGTGC ACCAGGACCT CTCAGACGAT 
GACATCAAGA GCGTGTTTGA GGCCTTTGGC AAGATCAAGT CCTGCACACT GGCCCGGGAC 
CCCACAACTG GCAAGCACAA GGGCTACGGC TTCATTGAGT ACGAGAAGGC CCAGTCGTCC 
CAAGATGCTG TGTCTTCCAT GAACCTCTTT GACCTGGGTG GCCAGTACTT GCGGGTGGGC 
AAGGCTGTCA CACCGCCCAT GCCCCTACTC ACACCAGCCA CGCCTGGAGG CCTCCCACCT 
GCCGCTGCTG TGGCAGCTGC TGCAGCCACT GCCAAGATCA CAGCTCAGGA AGCAGTGGCC 
GGAGCAGCGG TGCTGGGTAC CCTGGGCACA CCTGGACTGG TGTCCCCAGC ACTGACCCTG 
GCCCAGCCCC TGGGCACTTT GCCCCAGGCT GTCATGGCTG CCCAGGCACC TGGAGTCATC 
ACAGGTGTGA CCCCAGCCCG TCCTCCTATC CCGGTCACCA TCCCCTCGGT GGGAGTGGTG 
AACCCCATCC TGGCCAGCCC TCCAACGCTG GGTCTCCTGG AGCCCAAGAA GGAGAAGGAA 
GAAGAGGAGC TGTTTCCCGA GTCAGAGCGG CCAGAGATGC TGAGCGAGCA GGAGCACATG 
AGCATCTCGG GCAGTAGCGC CCGACACATG GTGATGCAGA AGCTGCTCCG CAAGCAGGAG 
TCTACAGTGA TGGTTCTGCG CAACATGGTG GACCCCAAGG ACATCGATGA TGACCTGGAA 
GGGGAGGTGA CAGAGGAGTG TGGCAAGTTC GGGGCCGTGA ACCGCGTCAT CATCTACCAA 
GAGAAACAAG GCGAGGAGGA GGATGCAGAA ATCATTGTCA AGATCTTTGT GGAGTTTTCC 
ATAGCCTCTG AGACTCATAA GGCCATCCAG GCCCTCAATG GCCGCTGGTT TGCTGGCCGC 
AAGGTGGTGG CTGAAGTGTA CGACCAGGAG CGTTTTGATA ACAGTGACCT CTCTGCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PUF60 ( XM_017013235.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016868724.1 Homo sapiens poly(U) binding splicing factor 60 (PUF60), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OHu97848
Clone ID Related Accession (Same CDS sequence)	XM_017013235.1
Accession Version	XM_017013235.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1755bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	poly(U)-binding-splicing factor PUF60 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000008.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCTTA AAAGTGTCTT CAGGAGAGAA CTGGGACCTG GGCTGGGCCA GAGCCTGTGG 
AAGGCACTGG AGCTGGGGCT GAGTTGTGGG CTGGCCAAGC AGGTCAATGG CCAGCAAGGA 
GGGGGGTCCG AGCCGGCGGC GGCGGCGGCA GTGGTGGCAG CGGGAGACAA ATGGAAACCT 
CCACAGGGCA CAGACTCCAT CAAGATGGAG AACGGGCAGA GCACAGCCGC CAAGCTGGGG 
CTGCCTCCCC TGACGCCCGA GCAGCAGGAG GCCCTTCAGA AGGCCAAGAA GTACGCCATG 
GAGCAGAGCA TCAAGAGTGT GCTGGTGAAG CAGACCATCG CGCACCAGCA GCAGCAGCTC 
ACCAACCTGC AGATGGCAGC AGTGACAATG GGCTTTGGAG ATCCTCTCTC ACCTTTGCAA 
TCGATGGCGG CTCAGCGGCA GCGGGCGCTG GCCATCATGT GCCGCGTCTA CGTGGGCTCT 
ATCTACTATG AGCTGGGGGA GGACACCATC CGCCAGGCCT TTGCCCCCTT TGGCCCCATC 
AAGAGCATCG ACATGTCCTG GGACTCCGTC ACCATGAAGC ACAAGGGCTT TGCCTTCGTG 
GAGTATGAGG TCCCCGAAGC TGCACAGCTG GCCTTGGAGC AGATGAACTC GGTGATGCTG 
GGGGGCAGGA ACATCAAGGT GGGCAGACCC AGCAACATAG GGCAGGCCCA GCCCATCATA 
GACCAGTTGG CTGAGGAGGC ACGGGCCTTC AACCGCATCT ACGTGGCCTC TGTGCACCAG 
GACCTCTCAG ACGATGACAT CAAGAGCGTG TTTGAGGCCT TTGGCAAGAT CAAGTCCTGC 
ACACTGGCCC GGGACCCCAC AACTGGCAAG CACAAGGGCT ACGGCTTCAT TGAGTACGAG 
AAGGCCCAGT CGTCCCAAGA TGCTGTGTCT TCCATGAACC TCTTTGACCT GGGTGGCCAG 
TACTTGCGGG TGGGCAAGGC TGTCACACCG CCCATGCCCC TACTCACACC AGCCACGCCT 
GGAGGCCTCC CACCTGCCGC TGCTGTGGCA GCTGCTGCAG CCACTGCCAA GATCACAGCT 
CAGGAAGCAG TGGCCGGAGC AGCGGTGCTG GGTACCCTGG GCACACCTGG ACTGGTGTCC 
CCAGCACTGA CCCTGGCCCA GCCCCTGGGC ACTTTGCCCC AGGCTGTCAT GGCTGCCCAG 
GCACCTGGAG TCATCACAGG TGTGACCCCA GCCCGTCCTC CTATCCCGGT CACCATCCCC 
TCGGTGGGAG TGGTGAACCC CATCCTGGCC AGCCCTCCAA CGCTGGGTCT CCTGGAGCCC 
AAGAAGGAGA AGGAAGAAGA GGAGCTGTTT CCCGAGTCAG AGCGGCCAGA GATGCTGAGC 
GAGCAGGAGC ACATGAGCAT CTCGGGCAGT AGCGCCCGAC ACATGGTGAT GCAGAAGCTG 
CTCCGCAAGC AGGAGTCTAC AGTGATGGTT CTGCGCAACA TGGTGGACCC CAAGGACATC 
GATGATGACC TGGAAGGGGA GGTGACAGAG GAGTGTGGCA AGTTCGGGGC CGTGAACCGC 
GTCATCATCT ACCAAGAGAA ACAAGGCGAG GAGGAGGATG CAGAAATCAT TGTCAAGATC 
TTTGTGGAGT TTTCCATAGC CTCTGAGACT CATAAGGCCA TCCAGGCCCT CAATGGCCGC 
TGGTTTGCTG GCCGCAAGGT GGTGGCTGAA GTGTACGACC AGGAGCGTTT TGATAACAGT 
GACCTCTCTG CGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016868724.1
CDS	111..1865
Translation	MLLKSVFRRELGPGLGQSLWKALELGLSCGLAKQVNGQQGGGSEPAAAAAVVAAGDKWKPPQGTDSIKMENGQSTAAKLGLPPLTPEQQEALQKAKKYAMEQSIKSVLVKQTIAHQQQQLTNLQMAAVTMGFGDPLSPLQSMAAQRQRALAIMCRVYVGSIYYELGEDTIRQAFAPFGPIKSIDMSWDSVTMKHKGFAFVEYEVPEAAQLALEQMNSVMLGGRNIKVGRPSNIGQAQPIIDQLAEEARAFNRIYVASVHQDLSDDDIKSVFEAFGKIKSCTLARDPTTGKHKGYGFIEYEKAQSSQDAVSSMNLFDLGGQYLRVGKAVTPPMPLLTPATPGGLPPAAAVAAAAATAKITAQEAVAGAAVLGTLGTPGLVSPALTLAQPLGTLPQAVMAAQAPGVITGVTPARPPIPVTIPSVGVVNPILASPPTLGLLEPKKEKEEEELFPESERPEMLSEQEHMSISGSSARHMVMQKLLRKQESTVMVLRNMVDPKDIDDDLEGEVTEECGKFGAVNRVIIYQEKQGEEEDAEIIVKIFVEFSIASETHKAIQALNGRWFAGRKVVAEVYDQERFDNSDLSA

Target ORF information:

RefSeq Version	XM_017013235.1
Organism	Homo sapiens(human)
Definition	Homo sapiens poly(U) binding splicing factor 60 (PUF60), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017013235.1

ORF Insert Sequence:

ATGCTGCTTA AAAGTGTCTT CAGGAGAGAA CTGGGACCTG GGCTGGGCCA GAGCCTGTGG 
AAGGCACTGG AGCTGGGGCT GAGTTGTGGG CTGGCCAAGC AGGTCAATGG CCAGCAAGGA 
GGGGGGTCCG AGCCGGCGGC GGCGGCGGCA GTGGTGGCAG CGGGAGACAA ATGGAAACCT 
CCACAGGGCA CAGACTCCAT CAAGATGGAG AACGGGCAGA GCACAGCCGC CAAGCTGGGG 
CTGCCTCCCC TGACGCCCGA GCAGCAGGAG GCCCTTCAGA AGGCCAAGAA GTACGCCATG 
GAGCAGAGCA TCAAGAGTGT GCTGGTGAAG CAGACCATCG CGCACCAGCA GCAGCAGCTC 
ACCAACCTGC AGATGGCAGC AGTGACAATG GGCTTTGGAG ATCCTCTCTC ACCTTTGCAA 
TCGATGGCGG CTCAGCGGCA GCGGGCGCTG GCCATCATGT GCCGCGTCTA CGTGGGCTCT 
ATCTACTATG AGCTGGGGGA GGACACCATC CGCCAGGCCT TTGCCCCCTT TGGCCCCATC 
AAGAGCATCG ACATGTCCTG GGACTCCGTC ACCATGAAGC ACAAGGGCTT TGCCTTCGTG 
GAGTATGAGG TCCCCGAAGC TGCACAGCTG GCCTTGGAGC AGATGAACTC GGTGATGCTG 
GGGGGCAGGA ACATCAAGGT GGGCAGACCC AGCAACATAG GGCAGGCCCA GCCCATCATA 
GACCAGTTGG CTGAGGAGGC ACGGGCCTTC AACCGCATCT ACGTGGCCTC TGTGCACCAG 
GACCTCTCAG ACGATGACAT CAAGAGCGTG TTTGAGGCCT TTGGCAAGAT CAAGTCCTGC 
ACACTGGCCC GGGACCCCAC AACTGGCAAG CACAAGGGCT ACGGCTTCAT TGAGTACGAG 
AAGGCCCAGT CGTCCCAAGA TGCTGTGTCT TCCATGAACC TCTTTGACCT GGGTGGCCAG 
TACTTGCGGG TGGGCAAGGC TGTCACACCG CCCATGCCCC TACTCACACC AGCCACGCCT 
GGAGGCCTCC CACCTGCCGC TGCTGTGGCA GCTGCTGCAG CCACTGCCAA GATCACAGCT 
CAGGAAGCAG TGGCCGGAGC AGCGGTGCTG GGTACCCTGG GCACACCTGG ACTGGTGTCC 
CCAGCACTGA CCCTGGCCCA GCCCCTGGGC ACTTTGCCCC AGGCTGTCAT GGCTGCCCAG 
GCACCTGGAG TCATCACAGG TGTGACCCCA GCCCGTCCTC CTATCCCGGT CACCATCCCC 
TCGGTGGGAG TGGTGAACCC CATCCTGGCC AGCCCTCCAA CGCTGGGTCT CCTGGAGCCC 
AAGAAGGAGA AGGAAGAAGA GGAGCTGTTT CCCGAGTCAG AGCGGCCAGA GATGCTGAGC 
GAGCAGGAGC ACATGAGCAT CTCGGGCAGT AGCGCCCGAC ACATGGTGAT GCAGAAGCTG 
CTCCGCAAGC AGGAGTCTAC AGTGATGGTT CTGCGCAACA TGGTGGACCC CAAGGACATC 
GATGATGACC TGGAAGGGGA GGTGACAGAG GAGTGTGGCA AGTTCGGGGC CGTGAACCGC 
GTCATCATCT ACCAAGAGAA ACAAGGCGAG GAGGAGGATG CAGAAATCAT TGTCAAGATC 
TTTGTGGAGT TTTCCATAGC CTCTGAGACT CATAAGGCCA TCCAGGCCCT CAATGGCCGC 
TGGTTTGCTG GCCGCAAGGT GGTGGCTGAA GTGTACGACC AGGAGCGTTT TGATAACAGT 
GACCTCTCTG CGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Exome sequencing reveals NAA15 and PUF60 as candidate genes associated with intellectual disability.
American journal of medical genetics. Part B, Neuropsychiatric genetics : the official publication of the International Society of Psychiatric Genetics177(1)10-20(2018 Jan)
Zhao JJ,Halvardson J,Zander CS,Zaghlool A,Georgii-Hemming P,M?nsson E,Brandberg G,S?vmarker HE,Frykholm C,Kuchinskaya E,Thuresson AC,Feuk L

A de novo PUF60 mutation in a child with a syndromic form of coloboma and persistent fetal vasculature.
Ophthalmic genetics38(6)590-592(2017 Dec)
Graziano C,Gusson E,Severi G,Isidori F,Wischmeijer A,Brugnara M,Seri M,Rossi C

Involvement of PUF60 in Transcriptional and Post-transcriptional Regulation of Hepatitis B Virus Pregenomic RNA Expression.
Scientific reports7(1)12874(2017 Oct)
Sun S,Nakashima K,Ito M,Li Y,Chida T,Takahashi H,Watashi K,Sawasaki T,Wakita T,Suzuki T

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PUF60 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

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