NID2 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	NID2
Entrez Gene ID	22795
Full Name	nidogen 2
Synonyms	NID-2
General protein information	Preferred Names nidogen 2 Names nidogen-2 osteonidogen
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 14 Map Location: 14q22.1
Summary	This gene encodes a member of the nidogen family of basement membrane proteins. This protein is a cell-adhesion protein that binds collagens I and IV and laminin and may be involved in maintaining the structure of the basement membrane.[provided by RefSeq, Jun 2010].
Disorder MIM:	605399

mRNA and Protein(s)

mRNA	Protein	Name
XM_005267406.4	XP_005267463.1	nidogen-2 isoform X2
XM_005267405.4	XP_005267462.1	nidogen-2 isoform X1
NM_007361.3	NP_031387.3	nidogen-2 precursor
XM_005267405.5	XP_005267462.1	nidogen-2 isoform X1
XM_005267406.5	XP_005267463.1	nidogen-2 isoform X2
XM_005267407.5	XP_005267464.1	nidogen-2 isoform X3
NM_007361.4	NP_031387.3	nidogen-2 precursor
XM_005267407.4	XP_005267464.1	nidogen-2 isoform X3

Pathways from BioSystems

Homologies

Homo sapiens (human)	NID2	NP_031387.3
Canis lupus familiaris (dog)	NID2	XP_537445.4
Mus musculus (house mouse)	Nid2	NP_032721.2
Danio rerio (zebrafish)	nid2a	XP_005169909.1
Gallus gallus (chicken)	NID2	XP_421471.4
	NID2	XP_001098764.1
Xenopus tropicalis (tropical clawed frog)	nid2	NP_001090732.1
Pan troglodytes (chimpanzee)	NID2	XP_001158556.2
Rattus norvegicus (Norway rat)	Nid2	NP_001012005.2

Gene Ontology
Bibliography

Related articles in PubMed

Elevated levels of serum nidogen-2 in esophageal squamous cell carcinoma.
Chai AWY, Cheung AKL, Dai W, Ko JMY, Lee NPY, Chan KT, Law SY, Lung ML
Cancer biomarkers : section A of Disease markers(2017 Dec)

Characterization of the Extracellular Matrix of Normal and Diseased Tissues Using Proteomics.
Naba A, Pearce OMT, Del Rosario A, Ma D, Ding H, Rajeeve V, Cutillas PR, Balkwill FR, Hynes RO
Journal of proteome research16(8)3083-3091(2017 Aug)

Comprehensive proteomic characterization of stem cell-derived extracellular matrices.
Ragelle H, Naba A, Larson BL, Zhou F, Priji? M, Whittaker CA, Del Rosario A, Langer R, Hynes RO, Anderson DG
Biomaterials128147-159(2017 06)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Knockdown of nidogen-2 reduced SOX9 expression, whereas it up-regulated RUNX2 expression
Title: Laminins and Nidogens in the Pericellular Matrix of Chondrocytes: Their Role in Osteoarthritis and Chondrogenic Differentiation.

The cleavage of nidogen-1 and -2 is partially inhibited in human tumor samples.
Title: Contribution of ADAMTS1 as a tumor suppressor gene in human breast carcinoma. Linking its tumor inhibitory properties to its proteolytic activity on nidogen-1 and nidogen-2.

combined methylation analysis of Twist homolog 1 and nidogen-2 genes may be a simple, noninvasive, sensitive, and specific method for detecting cancer cells in urine.
Title: Hypermethylation of TWIST1 and NID2 in tumor tissues and voided urine in urinary bladder cancer patients.

Decreased nidogen-2 expression is associated with hepatocellular carcinoma
Title: Clinical significance of decreased nidogen-2 expression in the tumor tissue and serum of patients with hepatocellular carcinoma.

NID2 promoter hypermethylation is associated with oral cavity squamous cell carcinoma.
Title: NID2 and HOXA9 promoter hypermethylation as biomarkers for prevention and early detection in oral cavity squamous cell carcinoma tissues and saliva.

The following NID2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NID2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu09649	NM_007361.4 Latest version!	Homo sapiens nidogen 2 (NID2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$659.50-$923.30 $1319.00
OHu47583	XM_005267407.5 Latest version!	Homo sapiens nidogen 2 (NID2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu47580	XM_005267405.5 Latest version!	Homo sapiens nidogen 2 (NID2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
OHu47582	XM_005267406.5 Latest version!	Homo sapiens nidogen 2 (NID2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
View Old Accession Versions >>
OHu47580	XM_005267405.4	Homo sapiens nidogen 2 (NID2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
OHu47582	XM_005267406.4	Homo sapiens nidogen 2 (NID2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
OHu09649	NM_007361.3	Homo sapiens nidogen 2 (NID2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$659.50-$923.30 $1319.00
OHu47583	XM_005267407.4	Homo sapiens nidogen 2 (NID2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NID2 ( NM_007361.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_031387.3 Homo sapiens nidogen 2 (NID2), mRNA.

Price & Availability↑

CloneID	OHu09649
Clone ID Related Accession (Same CDS sequence)	NM_007361.3 , NM_007361.4
Accession Version	NM_007361.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4128bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	nidogen-2 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BM719690.1, AL118557.5, AJ223500.1 and CA413165.1. On Apr 11, 2007 this sequence version replaced NM_007361.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.169212.1, SRR1803614.82441.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGGGGG ACCGGGTGGC CGGGCGGCCG GTGCTGTCGT CGTTACCAGT GCTACTGCTG 
CTGCCGTTGC TAATGTTGCG GGCCGCGGCG CTGCACCCAG ACGAGCTCTT CCCACACGGG 
GAGTCGTGGG GGGACCAGCT CCTGCAGGAA GGCGACGACG AAAGCTCAGC CGTGGTGAAG 
CTGGCGAATC CCCTGCACTT CTACGAAGCC CGATTCAGCA ACCTCTACGT GGGCACCAAC 
GGCATCATCT CCACTCAGGA CTTCCCCAGG GAAACGCAGT ATGTGGACTA TGATTTCCCC 
ACCGACTTCC CGGCCATCGC CCCTTTTCTG GCGGACATCG ACACGAGCCA CGGCAGAGGC 
CGAGTCCTGT ACCGAGAGGA CACCTCCCCC GCAGTGCTGG GCCTGGCCGC CCGCTATGTG 
CGCGCTGGCT TCCCGCGCTC TGCGCGCTTT ACCCCCACCC ACGCCTTCCT GGCCACCTGG 
GAGCAGGTAG GCGCTTACGA GGAGGTCAAG CGCGGGGCGC TGCCCTCGGG AGAGCTGAAC 
ACTTTCCAGG CAGTTTTGGC ATCTGATGGG TCTGATAGCT ACGCCCTCTT TCTTTATCCT 
GCCAACGGCC TGCAGTTCCT TGGAACCCGC CCCAAAGAGT CTTACAATGT CCAGCTTCAG 
CTTCCAGCTC GGGTGGGCTT CTGCCGAGGG GAGGCTGATG ATCTGAAGTC AGAAGGACCA 
TATTTCAGCT TGACTAGCAC TGAACAGTCT GTGAAAAATC TCTATCAACT AAGCAACCTG 
GGGATCCCTG GAGTGTGGGC TTTCCATATC GGCAGCACTT CCCCGTTGGA CAATGTCAGG 
CCAGCTGCAG TTGGAGACCT TTCCGCTGCC CACTCTTCTG TTCCCCTGGG ACGTTCCTTC 
AGCCATGCTA CAGCCCTGGA AAGTGACTAT AATGAGGACA ATTTGGATTA CTATGATGTG 
AATGAGGAGG AAGCTGAATA CCTTCCGGGT GAACCAGAGG AGGCATTGAA TGGCCACAGC 
AGCATTGATG TTTCCTTCCA ATCCAAAGTG GATACAAAGC CTTTAGAGGA ATCTTCCACC 
TTGGATCCTC ACACCAAAGA AGGAACATCT CTGGGAGAGG TAGGGGGCCC AGATTTAAAA 
GGCCAAGTTG AGCCCTGGGA TGAGAGAGAG ACCAGAAGCC CAGCTCCACC AGAGGTAGAC 
AGAGATTCAC TGGCTCCTTC CTGGGAAACC CCACCACCGT ACCCCGAAAA CGGAAGCATC 
CAGCCCTACC CAGATGGAGG GCCAGTGCCT TCGGAAATGG ATGTTCCCCC AGCTCATCCT 
GAAGAAGAAA TTGTTCTTCG AAGTTACCCT GCTTCAGGTC ACACTACACC CTTAAGTCGA 
GGGACGTATG AGGTGGGACT GGAAGACAAC ATAGGTTCCA ACACCGAGGT CTTCACGTAT 
AATGCTGCCA ACAAGGAAAC CTGTGAACAC AACCACAGAC AATGCTCCCG GCATGCCTTC 
TGCACGGACT ATGCCACTGG CTTCTGCTGC CACTGCCAAT CCAAGTTTTA TGGAAATGGG 
AAGCACTGTC TGCCTGAAGG GGCACCTCAC CGAGTGAATG GGAAAGTGAG TGGCCACCTC 
CACGTGGGCC ATACACCCGT GCACTTCACT GATGTGGACC TGCATGCGTA TATCGTGGGC 
AATGATGGCA GAGCCTACAC GGCCATCAGC CACATCCCAC AGCCAGCAGC CCAGGCCCTC 
CTCCCCCTCA CACCAATTGG AGGCCTGTTT GGCTGGCTCT TTGCTTTAGA AAAACCTGGC 
TCTGAGAACG GCTTCAGCCT CGCAGGTGCT GCCTTTACCC ATGACATGGA AGTTACATTC 
TACCCGGGAG AGGAGACGGT TCGTATCACT CAAACTGCTG AGGGACTTGA CCCAGAGAAC 
TACCTGAGCA TTAAGACCAA CATTCAAGGC CAGGTGCCTT ACGTCTCAGC AAATTTCACA 
GCCCACATCT CTCCCTACAA GGAGCTGTAC CACTACTCCG ACTCCACTGT GACCTCTACA 
AGTTCCAGAG ACTACTCTCT GACTTTTGGT GCAATCAACC AAACATGGTC CTACCGCATC 
CACCAGAACA TCACTTACCA GGTGTGCAGG CACGCCCCCA GACACCCGTC CTTCCCCACC 
ACCCAGCAGC TGAACGTGGA CCGGGTCTTT GCCTTGTATA ATGACGAAGA AAGAGTGCTT 
AGATTTGCTG TGACCAATCA AATTGGCCCG GTCAAAGAGG ATTCAGACCC CACTCCGGGG 
AATCCTTGCT ATGATGGGAG CCACATGTGT GACACAACAG CACGGTGCCA TCCAGGGACA 
GGTGTAGATT ACACCTGTGA GTGCGCATCT GGGTACCAGG GAGATGGACG GAACTGTGTG 
GATGAAAATG AATGTGCAAC TGGCTTTCAT CGCTGTGGCC CCAACTCTGT ATGTATCAAC 
TTGCCTGGAA GCTACAGGTG TGAGTGCCGG AGTGGTTATG AGTTTGCAGA TGACCGGCAT 
ACTTGCATCT TGATCACCCC ACCTGCCAAC CCCTGTGAGG ATGGCAGTCA TACCTGTGCT 
CCTGCTGGGC AGGCCCGGTG TGTTCACCAT GGAGGCAGCA CGTTCAGCTG TGCCTGCCTG 
CCTGGTTATG CCGGCGATGG GCACCAGTGC ACTGATGTAG ATGAATGCTC AGAAAACAGA 
TGTCACCCTG CAGCTACCTG CTACAATACT CCTGGTTCCT TCTCCTGCCG TTGTCAACCC 
GGATATTATG GGGATGGATT TCAGTGCATA CCTGACTCCA CCTCAAGCCT GACACCCTGT 
GAACAACAGC AGCGCCATGC CCAGGCCCAG TATGCCTACC CTGGGGCCCG GTTCCACATC 
CCCCAATGCG ACGAGCAGGG CAACTTCCTG CCCCTACAGT GTCATGGCAG CACTGGTTTC 
TGCTGGTGCG TGGACCCTGA TGGTCATGAA GTTCCTGGTA CCCAGACTCC ACCTGGCTCC 
ACCCCGCCTC ACTGTGGACC ATCACCAGAG CCCACCCAGA GGCCCCCGAC CATCTGTGAG 
CGCTGGAGGG AAAACCTGCT GGAGCACTAC GGTGGCACCC CCCGGGATGA CCAGTACGTG 
CCCCAGTGCG ATGACCTGGG CCACTTCATC CCCCTGCAGT GCCACGGAAA GAGCGACTTC 
TGCTGGTGTG TGGACAAAGA TGGCAGAGAG GTGCAGGGCA CCCGCTCCCA GCCAGGCACC 
ACCCCTGCGT GTATACCCAC CGTCGCTCCA CCCATGGTCC GGCCCACGCC CCGGCCAGAT 
GTGACCCCTC CATCTGTGGG CACCTTCCTG CTCTATACTC AGGGCCAGCA GATTGGCTAC 
TTACCCCTCA ATGGCACCAG GCTTCAGAAG GATGCAGCTA AGACCCTGCT GTCTCTGCAT 
GGCTCCATAA TCGTGGGAAT TGATTACGAC TGCCGGGAGA GGATGGTGTA CTGGACAGAT 
GTTGCTGGAC GGACAATCAG CCGTGCTGGT CTGGAACTGG GAGCAGAGCC TGAGACGATC 
GTGAATTCAG GTCTGATAAG CCCTGAAGGA CTTGCCATAG ACCACATCCG CAGAACAATG 
TACTGGACGG ACAGTGTCCT GGATAAGATA GAGAGCGCCC TGCTGGATGG CTCTGAGCGC 
AAGGTCCTCT TCTACACAGA TCTGGTGAAT CCCCGTGCCA TCGCTGTGGA TCCAATCCGA 
GGCAACTTGT ACTGGACAGA CTGGAATAGA GAAGCTCCTA AAATTGAAAC GTCATCTTTA 
GATGGAGAAA ACAGAAGAAT TCTGATCAAT ACAGACATTG GATTGCCCAA TGGCTTAACC 
TTTGACCCTT TCTCTAAACT GCTCTGCTGG GCAGATGCAG GAACCAAAAA ACTGGAGTGT 
ACACTACCTG ATGGAACTGG ACGGCGTGTC ATTCAAAACA ACCTCAAGTA CCCCTTCAGC 
ATCGTAAGCT ATGCAGATCA CTTCTACCAC ACAGACTGGA GGAGGGATGG TGTTGTATCA 
GTAAATAAAC ATAGTGGCCA GTTTACTGAT GAGTATCTCC CAGAACAACG ATCTCACCTC 
TACGGGATAA CTGCAGTCTA CCCCTACTGC CCAACAGGAA GAAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_031387.3
CDS	235..4362
Translation	MEGDRVAGRPVLSSLPVLLLLPLLMLRAAALHPDELFPHGESWGDQLLQEGDDESSAVVKLANPLHFYEARFSNLYVGTNGIISTQDFPRETQYVDYDFPTDFPAIAPFLADIDTSHGRGRVLYREDTSPAVLGLAARYVRAGFPRSARFTPTHAFLATWEQVGAYEEVKRGALPSGELNTFQAVLASDGSDSYALFLYPANGLQFLGTRPKESYNVQLQLPARVGFCRGEADDLKSEGPYFSLTSTEQSVKNLYQLSNLGIPGVWAFHIGSTSPLDNVRPAAVGDLSAAHSSVPLGRSFSHATALESDYNEDNLDYYDVNEEEAEYLPGEPEEALNGHSSIDVSFQSKVDTKPLEESSTLDPHTKEGTSLGEVGGPDLKGQVEPWDERETRSPAPPEVDRDSLAPSWETPPPYPENGSIQPYPDGGPVPSEMDVPPAHPEEEIVLRSYPASGHTTPLSRGTYEVGLEDNIGSNTEVFTYNAANKETCEHNHRQCSRHAFCTDYATGFCCHCQSKFYGNGKHCLPEGAPHRVNGKVSGHLHVGHTPVHFTDVDLHAYIVGNDGRAYTAISHIPQPAAQALLPLTPIGGLFGWLFALEKPGSENGFSLAGAAFTHDMEVTFYPGEETVRITQTAEGLDPENYLSIKTNIQGQVPYVSANFTAHISPYKELYHYSDSTVTSTSSRDYSLTFGAINQTWSYRIHQNITYQVCRHAPRHPSFPTTQQLNVDRVFALYNDEERVLRFAVTNQIGPVKEDSDPTPGNPCYDGSHMCDTTARCHPGTGVDYTCECASGYQGDGRNCVDENECATGFHRCGPNSVCINLPGSYRCECRSGYEFADDRHTCILITPPANPCEDGSHTCAPAGQARCVHHGGSTFSCACLPGYAGDGHQCTDVDECSENRCHPAATCYNTPGSFSCRCQPGYYGDGFQCIPDSTSSLTPCEQQQRHAQAQYAYPGARFHIPQCDEQGNFLPLQCHGSTGFCWCVDPDGHEVPGTQTPPGSTPPHCGPSPEPTQRPPTICERWRENLLEHYGGTPRDDQYVPQCDDLGHFIPLQCHGKSDFCWCVDKDGREVQGTRSQPGTTPACIPTVAPPMVRPTPRPDVTPPSVGTFLLYTQGQQIGYLPLNGTRLQKDAAKTLLSLHGSIIVGIDYDCRERMVYWTDVAGRTISRAGLELGAEPETIVNSGLISPEGLAIDHIRRTMYWTDSVLDKIESALLDGSERKVLFYTDLVNPRAIAVDPIRGNLYWTDWNREAPKIETSSLDGENRRILINTDIGLPNGLTFDPFSKLLCWADAGTKKLECTLPDGTGRRVIQNNLKYPFSIVSYADHFYHTDWRRDGVVSVNKHSGQFTDEYLPEQRSHLYGITAVYPYCPTGRK

Target ORF information:

RefSeq Version	NM_007361.3
Organism	Homo sapiens(human)
Definition	Homo sapiens nidogen 2 (NID2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_007361.3

ORF Insert Sequence:

ATGGAGGGGG ACCGGGTGGC CGGGCGGCCG GTGCTGTCGT CGTTACCAGT GCTACTGCTG 
CTGCCGTTGC TAATGTTGCG GGCCGCGGCG CTGCACCCAG ACGAGCTCTT CCCACACGGG 
GAGTCGTGGG GGGACCAGCT CCTGCAGGAA GGCGACGACG AAAGCTCAGC CGTGGTGAAG 
CTGGCGAATC CCCTGCACTT CTACGAAGCC CGATTCAGCA ACCTCTACGT GGGCACCAAC 
GGCATCATCT CCACTCAGGA CTTCCCCAGG GAAACGCAGT ATGTGGACTA TGATTTCCCC 
ACCGACTTCC CGGCCATCGC CCCTTTTCTG GCGGACATCG ACACGAGCCA CGGCAGAGGC 
CGAGTCCTGT ACCGAGAGGA CACCTCCCCC GCAGTGCTGG GCCTGGCCGC CCGCTATGTG 
CGCGCTGGCT TCCCGCGCTC TGCGCGCTTT ACCCCCACCC ACGCCTTCCT GGCCACCTGG 
GAGCAGGTAG GCGCTTACGA GGAGGTCAAG CGCGGGGCGC TGCCCTCGGG AGAGCTGAAC 
ACTTTCCAGG CAGTTTTGGC ATCTGATGGG TCTGATAGCT ACGCCCTCTT TCTTTATCCT 
GCCAACGGCC TGCAGTTCCT TGGAACCCGC CCCAAAGAGT CTTACAATGT CCAGCTTCAG 
CTTCCAGCTC GGGTGGGCTT CTGCCGAGGG GAGGCTGATG ATCTGAAGTC AGAAGGACCA 
TATTTCAGCT TGACTAGCAC TGAACAGTCT GTGAAAAATC TCTATCAACT AAGCAACCTG 
GGGATCCCTG GAGTGTGGGC TTTCCATATC GGCAGCACTT CCCCGTTGGA CAATGTCAGG 
CCAGCTGCAG TTGGAGACCT TTCCGCTGCC CACTCTTCTG TTCCCCTGGG ACGTTCCTTC 
AGCCATGCTA CAGCCCTGGA AAGTGACTAT AATGAGGACA ATTTGGATTA CTATGATGTG 
AATGAGGAGG AAGCTGAATA CCTTCCGGGT GAACCAGAGG AGGCATTGAA TGGCCACAGC 
AGCATTGATG TTTCCTTCCA ATCCAAAGTG GATACAAAGC CTTTAGAGGA ATCTTCCACC 
TTGGATCCTC ACACCAAAGA AGGAACATCT CTGGGAGAGG TAGGGGGCCC AGATTTAAAA 
GGCCAAGTTG AGCCCTGGGA TGAGAGAGAG ACCAGAAGCC CAGCTCCACC AGAGGTAGAC 
AGAGATTCAC TGGCTCCTTC CTGGGAAACC CCACCACCGT ACCCCGAAAA CGGAAGCATC 
CAGCCCTACC CAGATGGAGG GCCAGTGCCT TCGGAAATGG ATGTTCCCCC AGCTCATCCT 
GAAGAAGAAA TTGTTCTTCG AAGTTACCCT GCTTCAGGTC ACACTACACC CTTAAGTCGA 
GGGACGTATG AGGTGGGACT GGAAGACAAC ATAGGTTCCA ACACCGAGGT CTTCACGTAT 
AATGCTGCCA ACAAGGAAAC CTGTGAACAC AACCACAGAC AATGCTCCCG GCATGCCTTC 
TGCACGGACT ATGCCACTGG CTTCTGCTGC CACTGCCAAT CCAAGTTTTA TGGAAATGGG 
AAGCACTGTC TGCCTGAAGG GGCACCTCAC CGAGTGAATG GGAAAGTGAG TGGCCACCTC 
CACGTGGGCC ATACACCCGT GCACTTCACT GATGTGGACC TGCATGCGTA TATCGTGGGC 
AATGATGGCA GAGCCTACAC GGCCATCAGC CACATCCCAC AGCCAGCAGC CCAGGCCCTC 
CTCCCCCTCA CACCAATTGG AGGCCTGTTT GGCTGGCTCT TTGCTTTAGA AAAACCTGGC 
TCTGAGAACG GCTTCAGCCT CGCAGGTGCT GCCTTTACCC ATGACATGGA AGTTACATTC 
TACCCGGGAG AGGAGACGGT TCGTATCACT CAAACTGCTG AGGGACTTGA CCCAGAGAAC 
TACCTGAGCA TTAAGACCAA CATTCAAGGC CAGGTGCCTT ACGTCTCAGC AAATTTCACA 
GCCCACATCT CTCCCTACAA GGAGCTGTAC CACTACTCCG ACTCCACTGT GACCTCTACA 
AGTTCCAGAG ACTACTCTCT GACTTTTGGT GCAATCAACC AAACATGGTC CTACCGCATC 
CACCAGAACA TCACTTACCA GGTGTGCAGG CACGCCCCCA GACACCCGTC CTTCCCCACC 
ACCCAGCAGC TGAACGTGGA CCGGGTCTTT GCCTTGTATA ATGACGAAGA AAGAGTGCTT 
AGATTTGCTG TGACCAATCA AATTGGCCCG GTCAAAGAGG ATTCAGACCC CACTCCGGGG 
AATCCTTGCT ATGATGGGAG CCACATGTGT GACACAACAG CACGGTGCCA TCCAGGGACA 
GGTGTAGATT ACACCTGTGA GTGCGCATCT GGGTACCAGG GAGATGGACG GAACTGTGTG 
GATGAAAATG AATGTGCAAC TGGCTTTCAT CGCTGTGGCC CCAACTCTGT ATGTATCAAC 
TTGCCTGGAA GCTACAGGTG TGAGTGCCGG AGTGGTTATG AGTTTGCAGA TGACCGGCAT 
ACTTGCATCT TGATCACCCC ACCTGCCAAC CCCTGTGAGG ATGGCAGTCA TACCTGTGCT 
CCTGCTGGGC AGGCCCGGTG TGTTCACCAT GGAGGCAGCA CGTTCAGCTG TGCCTGCCTG 
CCTGGTTATG CCGGCGATGG GCACCAGTGC ACTGATGTAG ATGAATGCTC AGAAAACAGA 
TGTCACCCTG CAGCTACCTG CTACAATACT CCTGGTTCCT TCTCCTGCCG TTGTCAACCC 
GGATATTATG GGGATGGATT TCAGTGCATA CCTGACTCCA CCTCAAGCCT GACACCCTGT 
GAACAACAGC AGCGCCATGC CCAGGCCCAG TATGCCTACC CTGGGGCCCG GTTCCACATC 
CCCCAATGCG ACGAGCAGGG CAACTTCCTG CCCCTACAGT GTCATGGCAG CACTGGTTTC 
TGCTGGTGCG TGGACCCTGA TGGTCATGAA GTTCCTGGTA CCCAGACTCC ACCTGGCTCC 
ACCCCGCCTC ACTGTGGACC ATCACCAGAG CCCACCCAGA GGCCCCCGAC CATCTGTGAG 
CGCTGGAGGG AAAACCTGCT GGAGCACTAC GGTGGCACCC CCCGGGATGA CCAGTACGTG 
CCCCAGTGCG ATGACCTGGG CCACTTCATC CCCCTGCAGT GCCACGGAAA GAGCGACTTC 
TGCTGGTGTG TGGACAAAGA TGGCAGAGAG GTGCAGGGCA CCCGCTCCCA GCCAGGCACC 
ACCCCTGCGT GTATACCCAC CGTCGCTCCA CCCATGGTCC GGCCCACGCC CCGGCCAGAT 
GTGACCCCTC CATCTGTGGG CACCTTCCTG CTCTATACTC AGGGCCAGCA GATTGGCTAC 
TTACCCCTCA ATGGCACCAG GCTTCAGAAG GATGCAGCTA AGACCCTGCT GTCTCTGCAT 
GGCTCCATAA TCGTGGGAAT TGATTACGAC TGCCGGGAGA GGATGGTGTA CTGGACAGAT 
GTTGCTGGAC GGACAATCAG CCGTGCTGGT CTGGAACTGG GAGCAGAGCC TGAGACGATC 
GTGAATTCAG GTCTGATAAG CCCTGAAGGA CTTGCCATAG ACCACATCCG CAGAACAATG 
TACTGGACGG ACAGTGTCCT GGATAAGATA GAGAGCGCCC TGCTGGATGG CTCTGAGCGC 
AAGGTCCTCT TCTACACAGA TCTGGTGAAT CCCCGTGCCA TCGCTGTGGA TCCAATCCGA 
GGCAACTTGT ACTGGACAGA CTGGAATAGA GAAGCTCCTA AAATTGAAAC GTCATCTTTA 
GATGGAGAAA ACAGAAGAAT TCTGATCAAT ACAGACATTG GATTGCCCAA TGGCTTAACC 
TTTGACCCTT TCTCTAAACT GCTCTGCTGG GCAGATGCAG GAACCAAAAA ACTGGAGTGT 
ACACTACCTG ATGGAACTGG ACGGCGTGTC ATTCAAAACA ACCTCAAGTA CCCCTTCAGC 
ATCGTAAGCT ATGCAGATCA CTTCTACCAC ACAGACTGGA GGAGGGATGG TGTTGTATCA 
GTAAATAAAC ATAGTGGCCA GTTTACTGAT GAGTATCTCC CAGAACAACG ATCTCACCTC 
TACGGGATAA CTGCAGTCTA CCCCTACTGC CCAACAGGAA GAAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NID2 ( NM_007361.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_031387.3 Homo sapiens nidogen 2 (NID2), mRNA.

Price & Availability↑

CloneID	OHu09649
Clone ID Related Accession (Same CDS sequence)	NM_007361.3 , NM_007361.4
Accession Version	NM_007361.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4128bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Homo sapiens(human)
Product	nidogen-2 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BM719690.1, AL118557.5, AJ223500.1 and CA413165.1. On Nov 22, 2018 this sequence version replaced NM_007361.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.169212.1, AJ223500.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000216286.10/ ENSP00000216286.4 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGAGGGGG ACCGGGTGGC CGGGCGGCCG GTGCTGTCGT CGTTACCAGT GCTACTGCTG 
CTGCCGTTGC TAATGTTGCG GGCCGCGGCG CTGCACCCAG ACGAGCTCTT CCCACACGGG 
GAGTCGTGGG GGGACCAGCT CCTGCAGGAA GGCGACGACG AAAGCTCAGC CGTGGTGAAG 
CTGGCGAATC CCCTGCACTT CTACGAAGCC CGATTCAGCA ACCTCTACGT GGGCACCAAC 
GGCATCATCT CCACTCAGGA CTTCCCCAGG GAAACGCAGT ATGTGGACTA TGATTTCCCC 
ACCGACTTCC CGGCCATCGC CCCTTTTCTG GCGGACATCG ACACGAGCCA CGGCAGAGGC 
CGAGTCCTGT ACCGAGAGGA CACCTCCCCC GCAGTGCTGG GCCTGGCCGC CCGCTATGTG 
CGCGCTGGCT TCCCGCGCTC TGCGCGCTTT ACCCCCACCC ACGCCTTCCT GGCCACCTGG 
GAGCAGGTAG GCGCTTACGA GGAGGTCAAG CGCGGGGCGC TGCCCTCGGG AGAGCTGAAC 
ACTTTCCAGG CAGTTTTGGC ATCTGATGGG TCTGATAGCT ACGCCCTCTT TCTTTATCCT 
GCCAACGGCC TGCAGTTCCT TGGAACCCGC CCCAAAGAGT CTTACAATGT CCAGCTTCAG 
CTTCCAGCTC GGGTGGGCTT CTGCCGAGGG GAGGCTGATG ATCTGAAGTC AGAAGGACCA 
TATTTCAGCT TGACTAGCAC TGAACAGTCT GTGAAAAATC TCTATCAACT AAGCAACCTG 
GGGATCCCTG GAGTGTGGGC TTTCCATATC GGCAGCACTT CCCCGTTGGA CAATGTCAGG 
CCAGCTGCAG TTGGAGACCT TTCCGCTGCC CACTCTTCTG TTCCCCTGGG ACGTTCCTTC 
AGCCATGCTA CAGCCCTGGA AAGTGACTAT AATGAGGACA ATTTGGATTA CTATGATGTG 
AATGAGGAGG AAGCTGAATA CCTTCCGGGT GAACCAGAGG AGGCATTGAA TGGCCACAGC 
AGCATTGATG TTTCCTTCCA ATCCAAAGTG GATACAAAGC CTTTAGAGGA ATCTTCCACC 
TTGGATCCTC ACACCAAAGA AGGAACATCT CTGGGAGAGG TAGGGGGCCC AGATTTAAAA 
GGCCAAGTTG AGCCCTGGGA TGAGAGAGAG ACCAGAAGCC CAGCTCCACC AGAGGTAGAC 
AGAGATTCAC TGGCTCCTTC CTGGGAAACC CCACCACCGT ACCCCGAAAA CGGAAGCATC 
CAGCCCTACC CAGATGGAGG GCCAGTGCCT TCGGAAATGG ATGTTCCCCC AGCTCATCCT 
GAAGAAGAAA TTGTTCTTCG AAGTTACCCT GCTTCAGGTC ACACTACACC CTTAAGTCGA 
GGGACGTATG AGGTGGGACT GGAAGACAAC ATAGGTTCCA ACACCGAGGT CTTCACGTAT 
AATGCTGCCA ACAAGGAAAC CTGTGAACAC AACCACAGAC AATGCTCCCG GCATGCCTTC 
TGCACGGACT ATGCCACTGG CTTCTGCTGC CACTGCCAAT CCAAGTTTTA TGGAAATGGG 
AAGCACTGTC TGCCTGAAGG GGCACCTCAC CGAGTGAATG GGAAAGTGAG TGGCCACCTC 
CACGTGGGCC ATACACCCGT GCACTTCACT GATGTGGACC TGCATGCGTA TATCGTGGGC 
AATGATGGCA GAGCCTACAC GGCCATCAGC CACATCCCAC AGCCAGCAGC CCAGGCCCTC 
CTCCCCCTCA CACCAATTGG AGGCCTGTTT GGCTGGCTCT TTGCTTTAGA AAAACCTGGC 
TCTGAGAACG GCTTCAGCCT CGCAGGTGCT GCCTTTACCC ATGACATGGA AGTTACATTC 
TACCCGGGAG AGGAGACGGT TCGTATCACT CAAACTGCTG AGGGACTTGA CCCAGAGAAC 
TACCTGAGCA TTAAGACCAA CATTCAAGGC CAGGTGCCTT ACGTCTCAGC AAATTTCACA 
GCCCACATCT CTCCCTACAA GGAGCTGTAC CACTACTCCG ACTCCACTGT GACCTCTACA 
AGTTCCAGAG ACTACTCTCT GACTTTTGGT GCAATCAACC AAACATGGTC CTACCGCATC 
CACCAGAACA TCACTTACCA GGTGTGCAGG CACGCCCCCA GACACCCGTC CTTCCCCACC 
ACCCAGCAGC TGAACGTGGA CCGGGTCTTT GCCTTGTATA ATGACGAAGA AAGAGTGCTT 
AGATTTGCTG TGACCAATCA AATTGGCCCG GTCAAAGAGG ATTCAGACCC CACTCCGGGG 
AATCCTTGCT ATGATGGGAG CCACATGTGT GACACAACAG CACGGTGCCA TCCAGGGACA 
GGTGTAGATT ACACCTGTGA GTGCGCATCT GGGTACCAGG GAGATGGACG GAACTGTGTG 
GATGAAAATG AATGTGCAAC TGGCTTTCAT CGCTGTGGCC CCAACTCTGT ATGTATCAAC 
TTGCCTGGAA GCTACAGGTG TGAGTGCCGG AGTGGTTATG AGTTTGCAGA TGACCGGCAT 
ACTTGCATCT TGATCACCCC ACCTGCCAAC CCCTGTGAGG ATGGCAGTCA TACCTGTGCT 
CCTGCTGGGC AGGCCCGGTG TGTTCACCAT GGAGGCAGCA CGTTCAGCTG TGCCTGCCTG 
CCTGGTTATG CCGGCGATGG GCACCAGTGC ACTGATGTAG ATGAATGCTC AGAAAACAGA 
TGTCACCCTG CAGCTACCTG CTACAATACT CCTGGTTCCT TCTCCTGCCG TTGTCAACCC 
GGATATTATG GGGATGGATT TCAGTGCATA CCTGACTCCA CCTCAAGCCT GACACCCTGT 
GAACAACAGC AGCGCCATGC CCAGGCCCAG TATGCCTACC CTGGGGCCCG GTTCCACATC 
CCCCAATGCG ACGAGCAGGG CAACTTCCTG CCCCTACAGT GTCATGGCAG CACTGGTTTC 
TGCTGGTGCG TGGACCCTGA TGGTCATGAA GTTCCTGGTA CCCAGACTCC ACCTGGCTCC 
ACCCCGCCTC ACTGTGGACC ATCACCAGAG CCCACCCAGA GGCCCCCGAC CATCTGTGAG 
CGCTGGAGGG AAAACCTGCT GGAGCACTAC GGTGGCACCC CCCGGGATGA CCAGTACGTG 
CCCCAGTGCG ATGACCTGGG CCACTTCATC CCCCTGCAGT GCCACGGAAA GAGCGACTTC 
TGCTGGTGTG TGGACAAAGA TGGCAGAGAG GTGCAGGGCA CCCGCTCCCA GCCAGGCACC 
ACCCCTGCGT GTATACCCAC CGTCGCTCCA CCCATGGTCC GGCCCACGCC CCGGCCAGAT 
GTGACCCCTC CATCTGTGGG CACCTTCCTG CTCTATACTC AGGGCCAGCA GATTGGCTAC 
TTACCCCTCA ATGGCACCAG GCTTCAGAAG GATGCAGCTA AGACCCTGCT GTCTCTGCAT 
GGCTCCATAA TCGTGGGAAT TGATTACGAC TGCCGGGAGA GGATGGTGTA CTGGACAGAT 
GTTGCTGGAC GGACAATCAG CCGTGCTGGT CTGGAACTGG GAGCAGAGCC TGAGACGATC 
GTGAATTCAG GTCTGATAAG CCCTGAAGGA CTTGCCATAG ACCACATCCG CAGAACAATG 
TACTGGACGG ACAGTGTCCT GGATAAGATA GAGAGCGCCC TGCTGGATGG CTCTGAGCGC 
AAGGTCCTCT TCTACACAGA TCTGGTGAAT CCCCGTGCCA TCGCTGTGGA TCCAATCCGA 
GGCAACTTGT ACTGGACAGA CTGGAATAGA GAAGCTCCTA AAATTGAAAC GTCATCTTTA 
GATGGAGAAA ACAGAAGAAT TCTGATCAAT ACAGACATTG GATTGCCCAA TGGCTTAACC 
TTTGACCCTT TCTCTAAACT GCTCTGCTGG GCAGATGCAG GAACCAAAAA ACTGGAGTGT 
ACACTACCTG ATGGAACTGG ACGGCGTGTC ATTCAAAACA ACCTCAAGTA CCCCTTCAGC 
ATCGTAAGCT ATGCAGATCA CTTCTACCAC ACAGACTGGA GGAGGGATGG TGTTGTATCA 
GTAAATAAAC ATAGTGGCCA GTTTACTGAT GAGTATCTCC CAGAACAACG ATCTCACCTC 
TACGGGATAA CTGCAGTCTA CCCCTACTGC CCAACAGGAA GAAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_031387.3
CDS	66..4193
Translation	MEGDRVAGRPVLSSLPVLLLLPLLMLRAAALHPDELFPHGESWGDQLLQEGDDESSAVVKLANPLHFYEARFSNLYVGTNGIISTQDFPRETQYVDYDFPTDFPAIAPFLADIDTSHGRGRVLYREDTSPAVLGLAARYVRAGFPRSARFTPTHAFLATWEQVGAYEEVKRGALPSGELNTFQAVLASDGSDSYALFLYPANGLQFLGTRPKESYNVQLQLPARVGFCRGEADDLKSEGPYFSLTSTEQSVKNLYQLSNLGIPGVWAFHIGSTSPLDNVRPAAVGDLSAAHSSVPLGRSFSHATALESDYNEDNLDYYDVNEEEAEYLPGEPEEALNGHSSIDVSFQSKVDTKPLEESSTLDPHTKEGTSLGEVGGPDLKGQVEPWDERETRSPAPPEVDRDSLAPSWETPPPYPENGSIQPYPDGGPVPSEMDVPPAHPEEEIVLRSYPASGHTTPLSRGTYEVGLEDNIGSNTEVFTYNAANKETCEHNHRQCSRHAFCTDYATGFCCHCQSKFYGNGKHCLPEGAPHRVNGKVSGHLHVGHTPVHFTDVDLHAYIVGNDGRAYTAISHIPQPAAQALLPLTPIGGLFGWLFALEKPGSENGFSLAGAAFTHDMEVTFYPGEETVRITQTAEGLDPENYLSIKTNIQGQVPYVSANFTAHISPYKELYHYSDSTVTSTSSRDYSLTFGAINQTWSYRIHQNITYQVCRHAPRHPSFPTTQQLNVDRVFALYNDEERVLRFAVTNQIGPVKEDSDPTPGNPCYDGSHMCDTTARCHPGTGVDYTCECASGYQGDGRNCVDENECATGFHRCGPNSVCINLPGSYRCECRSGYEFADDRHTCILITPPANPCEDGSHTCAPAGQARCVHHGGSTFSCACLPGYAGDGHQCTDVDECSENRCHPAATCYNTPGSFSCRCQPGYYGDGFQCIPDSTSSLTPCEQQQRHAQAQYAYPGARFHIPQCDEQGNFLPLQCHGSTGFCWCVDPDGHEVPGTQTPPGSTPPHCGPSPEPTQRPPTICERWRENLLEHYGGTPRDDQYVPQCDDLGHFIPLQCHGKSDFCWCVDKDGREVQGTRSQPGTTPACIPTVAPPMVRPTPRPDVTPPSVGTFLLYTQGQQIGYLPLNGTRLQKDAAKTLLSLHGSIIVGIDYDCRERMVYWTDVAGRTISRAGLELGAEPETIVNSGLISPEGLAIDHIRRTMYWTDSVLDKIESALLDGSERKVLFYTDLVNPRAIAVDPIRGNLYWTDWNREAPKIETSSLDGENRRILINTDIGLPNGLTFDPFSKLLCWADAGTKKLECTLPDGTGRRVIQNNLKYPFSIVSYADHFYHTDWRRDGVVSVNKHSGQFTDEYLPEQRSHLYGITAVYPYCPTGRK

Target ORF information:

RefSeq Version	NM_007361.4
Organism	Homo sapiens(human)
Definition	Homo sapiens nidogen 2 (NID2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_007361.4

ORF Insert Sequence:

ATGGAGGGGG ACCGGGTGGC CGGGCGGCCG GTGCTGTCGT CGTTACCAGT GCTACTGCTG 
CTGCCGTTGC TAATGTTGCG GGCCGCGGCG CTGCACCCAG ACGAGCTCTT CCCACACGGG 
GAGTCGTGGG GGGACCAGCT CCTGCAGGAA GGCGACGACG AAAGCTCAGC CGTGGTGAAG 
CTGGCGAATC CCCTGCACTT CTACGAAGCC CGATTCAGCA ACCTCTACGT GGGCACCAAC 
GGCATCATCT CCACTCAGGA CTTCCCCAGG GAAACGCAGT ATGTGGACTA TGATTTCCCC 
ACCGACTTCC CGGCCATCGC CCCTTTTCTG GCGGACATCG ACACGAGCCA CGGCAGAGGC 
CGAGTCCTGT ACCGAGAGGA CACCTCCCCC GCAGTGCTGG GCCTGGCCGC CCGCTATGTG 
CGCGCTGGCT TCCCGCGCTC TGCGCGCTTT ACCCCCACCC ACGCCTTCCT GGCCACCTGG 
GAGCAGGTAG GCGCTTACGA GGAGGTCAAG CGCGGGGCGC TGCCCTCGGG AGAGCTGAAC 
ACTTTCCAGG CAGTTTTGGC ATCTGATGGG TCTGATAGCT ACGCCCTCTT TCTTTATCCT 
GCCAACGGCC TGCAGTTCCT TGGAACCCGC CCCAAAGAGT CTTACAATGT CCAGCTTCAG 
CTTCCAGCTC GGGTGGGCTT CTGCCGAGGG GAGGCTGATG ATCTGAAGTC AGAAGGACCA 
TATTTCAGCT TGACTAGCAC TGAACAGTCT GTGAAAAATC TCTATCAACT AAGCAACCTG 
GGGATCCCTG GAGTGTGGGC TTTCCATATC GGCAGCACTT CCCCGTTGGA CAATGTCAGG 
CCAGCTGCAG TTGGAGACCT TTCCGCTGCC CACTCTTCTG TTCCCCTGGG ACGTTCCTTC 
AGCCATGCTA CAGCCCTGGA AAGTGACTAT AATGAGGACA ATTTGGATTA CTATGATGTG 
AATGAGGAGG AAGCTGAATA CCTTCCGGGT GAACCAGAGG AGGCATTGAA TGGCCACAGC 
AGCATTGATG TTTCCTTCCA ATCCAAAGTG GATACAAAGC CTTTAGAGGA ATCTTCCACC 
TTGGATCCTC ACACCAAAGA AGGAACATCT CTGGGAGAGG TAGGGGGCCC AGATTTAAAA 
GGCCAAGTTG AGCCCTGGGA TGAGAGAGAG ACCAGAAGCC CAGCTCCACC AGAGGTAGAC 
AGAGATTCAC TGGCTCCTTC CTGGGAAACC CCACCACCGT ACCCCGAAAA CGGAAGCATC 
CAGCCCTACC CAGATGGAGG GCCAGTGCCT TCGGAAATGG ATGTTCCCCC AGCTCATCCT 
GAAGAAGAAA TTGTTCTTCG AAGTTACCCT GCTTCAGGTC ACACTACACC CTTAAGTCGA 
GGGACGTATG AGGTGGGACT GGAAGACAAC ATAGGTTCCA ACACCGAGGT CTTCACGTAT 
AATGCTGCCA ACAAGGAAAC CTGTGAACAC AACCACAGAC AATGCTCCCG GCATGCCTTC 
TGCACGGACT ATGCCACTGG CTTCTGCTGC CACTGCCAAT CCAAGTTTTA TGGAAATGGG 
AAGCACTGTC TGCCTGAAGG GGCACCTCAC CGAGTGAATG GGAAAGTGAG TGGCCACCTC 
CACGTGGGCC ATACACCCGT GCACTTCACT GATGTGGACC TGCATGCGTA TATCGTGGGC 
AATGATGGCA GAGCCTACAC GGCCATCAGC CACATCCCAC AGCCAGCAGC CCAGGCCCTC 
CTCCCCCTCA CACCAATTGG AGGCCTGTTT GGCTGGCTCT TTGCTTTAGA AAAACCTGGC 
TCTGAGAACG GCTTCAGCCT CGCAGGTGCT GCCTTTACCC ATGACATGGA AGTTACATTC 
TACCCGGGAG AGGAGACGGT TCGTATCACT CAAACTGCTG AGGGACTTGA CCCAGAGAAC 
TACCTGAGCA TTAAGACCAA CATTCAAGGC CAGGTGCCTT ACGTCTCAGC AAATTTCACA 
GCCCACATCT CTCCCTACAA GGAGCTGTAC CACTACTCCG ACTCCACTGT GACCTCTACA 
AGTTCCAGAG ACTACTCTCT GACTTTTGGT GCAATCAACC AAACATGGTC CTACCGCATC 
CACCAGAACA TCACTTACCA GGTGTGCAGG CACGCCCCCA GACACCCGTC CTTCCCCACC 
ACCCAGCAGC TGAACGTGGA CCGGGTCTTT GCCTTGTATA ATGACGAAGA AAGAGTGCTT 
AGATTTGCTG TGACCAATCA AATTGGCCCG GTCAAAGAGG ATTCAGACCC CACTCCGGGG 
AATCCTTGCT ATGATGGGAG CCACATGTGT GACACAACAG CACGGTGCCA TCCAGGGACA 
GGTGTAGATT ACACCTGTGA GTGCGCATCT GGGTACCAGG GAGATGGACG GAACTGTGTG 
GATGAAAATG AATGTGCAAC TGGCTTTCAT CGCTGTGGCC CCAACTCTGT ATGTATCAAC 
TTGCCTGGAA GCTACAGGTG TGAGTGCCGG AGTGGTTATG AGTTTGCAGA TGACCGGCAT 
ACTTGCATCT TGATCACCCC ACCTGCCAAC CCCTGTGAGG ATGGCAGTCA TACCTGTGCT 
CCTGCTGGGC AGGCCCGGTG TGTTCACCAT GGAGGCAGCA CGTTCAGCTG TGCCTGCCTG 
CCTGGTTATG CCGGCGATGG GCACCAGTGC ACTGATGTAG ATGAATGCTC AGAAAACAGA 
TGTCACCCTG CAGCTACCTG CTACAATACT CCTGGTTCCT TCTCCTGCCG TTGTCAACCC 
GGATATTATG GGGATGGATT TCAGTGCATA CCTGACTCCA CCTCAAGCCT GACACCCTGT 
GAACAACAGC AGCGCCATGC CCAGGCCCAG TATGCCTACC CTGGGGCCCG GTTCCACATC 
CCCCAATGCG ACGAGCAGGG CAACTTCCTG CCCCTACAGT GTCATGGCAG CACTGGTTTC 
TGCTGGTGCG TGGACCCTGA TGGTCATGAA GTTCCTGGTA CCCAGACTCC ACCTGGCTCC 
ACCCCGCCTC ACTGTGGACC ATCACCAGAG CCCACCCAGA GGCCCCCGAC CATCTGTGAG 
CGCTGGAGGG AAAACCTGCT GGAGCACTAC GGTGGCACCC CCCGGGATGA CCAGTACGTG 
CCCCAGTGCG ATGACCTGGG CCACTTCATC CCCCTGCAGT GCCACGGAAA GAGCGACTTC 
TGCTGGTGTG TGGACAAAGA TGGCAGAGAG GTGCAGGGCA CCCGCTCCCA GCCAGGCACC 
ACCCCTGCGT GTATACCCAC CGTCGCTCCA CCCATGGTCC GGCCCACGCC CCGGCCAGAT 
GTGACCCCTC CATCTGTGGG CACCTTCCTG CTCTATACTC AGGGCCAGCA GATTGGCTAC 
TTACCCCTCA ATGGCACCAG GCTTCAGAAG GATGCAGCTA AGACCCTGCT GTCTCTGCAT 
GGCTCCATAA TCGTGGGAAT TGATTACGAC TGCCGGGAGA GGATGGTGTA CTGGACAGAT 
GTTGCTGGAC GGACAATCAG CCGTGCTGGT CTGGAACTGG GAGCAGAGCC TGAGACGATC 
GTGAATTCAG GTCTGATAAG CCCTGAAGGA CTTGCCATAG ACCACATCCG CAGAACAATG 
TACTGGACGG ACAGTGTCCT GGATAAGATA GAGAGCGCCC TGCTGGATGG CTCTGAGCGC 
AAGGTCCTCT TCTACACAGA TCTGGTGAAT CCCCGTGCCA TCGCTGTGGA TCCAATCCGA 
GGCAACTTGT ACTGGACAGA CTGGAATAGA GAAGCTCCTA AAATTGAAAC GTCATCTTTA 
GATGGAGAAA ACAGAAGAAT TCTGATCAAT ACAGACATTG GATTGCCCAA TGGCTTAACC 
TTTGACCCTT TCTCTAAACT GCTCTGCTGG GCAGATGCAG GAACCAAAAA ACTGGAGTGT 
ACACTACCTG ATGGAACTGG ACGGCGTGTC ATTCAAAACA ACCTCAAGTA CCCCTTCAGC 
ATCGTAAGCT ATGCAGATCA CTTCTACCAC ACAGACTGGA GGAGGGATGG TGTTGTATCA 
GTAAATAAAC ATAGTGGCCA GTTTACTGAT GAGTATCTCC CAGAACAACG ATCTCACCTC 
TACGGGATAA CTGCAGTCTA CCCCTACTGC CCAACAGGAA GAAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NID2 ( XM_005267407.5 ) cDNA ORF clone, Homo sapiens(human) -> XP_005267464.1 Homo sapiens nidogen 2 (NID2), transcript variant X3, mRNA.

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CloneID	OHu47583
Clone ID Related Accession (Same CDS sequence)	XM_005267407.5 , XM_005267407.4
Accession Version	XM_005267407.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3984bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	nidogen-2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000014.9) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_005267407.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGGGG ACCGGGTGGC CGGGCGGCCG GTGCTGTCGT CGTTACCAGT GCTACTGCTG 
CTGCCGTTGC TAATGTTGCG GGCCGCGGCG CTGCACCCAG ACGAGCTCTT CCCACACGGG 
GAGTCGTGGG GGGACCAGCT CCTGCAGGAA GGCGACGACG AAAGCTCAGC CGTGGTGAAG 
CTGGCGAATC CCCTGCACTT CTACGAAGCC CGATTCAGCA ACCTCTACGT GGGCACCAAC 
GGCATCATCT CCACTCAGGA CTTCCCCAGG GAAACGCAGT ATGTGGACTA TGATTTCCCC 
ACCGACTTCC CGGCCATCGC CCCTTTTCTG GCGGACATCG ACACGAGCCA CGGCAGAGGC 
CGAGTCCTGT ACCGAGAGGA CACCTCCCCC GCAGTGCTGG GCCTGGCCGC CCGCTATGTG 
CGCGCTGGCT TCCCGCGCTC TGCGCGCTTT ACCCCCACCC ACGCCTTCCT GGCCACCTGG 
GAGCAGGTAG GCGCTTACGA GGAGGTCAAG CGCGGGGCGC TGCCCTCGGG AGAGCTGAAC 
ACTTTCCAGG CAGTTTTGGC ATCTGATGGG TCTGATAGCT ACGCCCTCTT TCTTTATCCT 
GCCAACGGCC TGCAGTTCCT TGGAACCCGC CCCAAAGAGT CTTACAATGT CCAGCTTCAG 
CTTCCAGCTC GGGTGGGCTT CTGCCGAGGG GAGGCTGATG ATCTGAAGTC AGAAGGACCA 
TATTTCAGCT TGACTAGCAC TGAACAGTCT GTGAAAAATC TCTATCAACT AAGCAACCTG 
GGGATCCCTG GAGTGTGGGC TTTCCATATC GGCAGCACTT CCCCGTTGGA CAATGTCAGG 
CCAGCTGCAG TTGGAGACCT TTCCGCTGCC CACTCTTCTG TTCCCCTGGG ACGTTCCTTC 
AGCCATGCTA CAGCCCTGGA AAGTGACTAT AATGAGGACA ATTTGGATTA CTATGATGTG 
AATGAGGAGG AAGCTGAATA CCTTCCGGGT GAACCAGAGG AGGCATTGAA TGGCCACAGC 
AGCATTGATG TTTCCTTCCA ATCCAAAGTG GATACAAAGC CTTTAGAGGA ATCTTCCACC 
TTGGATCCTC ACACCAAAGA AGGAACATCT CTGGGAGAGG TAGGGGGCCC AGATTTAAAA 
GGCCAAGTTG AGCCCTGGGA TGAGAGAGAG ACCAGAAGCC CAGCTCCACC AGAGGTAGAC 
AGAGATTCAC TGGCTCCTTC CTGGGAAACC CCACCACCGT ACCCCGAAAA CGGAAGCATC 
CAGCCCTACC CAGATGGAGG GCCAGTGCCT TCGGAAATGG ATGTTCCCCC AGCTCATCCT 
GAAGAAGAAA TTGTTCTTCG AAGTTACCCT GCTTCAGGTC ACACTACACC CTTAAGTCGA 
GGGACGTATG AGGTGGGACT GGAAGACAAC ATAGGTTCCA ACACCGAGGT CTTCACGTAT 
AATGCTGCCA ACAAGGAAAC CTGTGAACAC AACCACAGAC AATGCTCCCG GCATGCCTTC 
TGCACGGACT ATGCCACTGG CTTCTGCTGC CACTGCCAAT CCAAGTTTTA TGGAAATGGG 
AAGCACTGTC TGCCTGAAGG GGCACCTCAC CGAGTGAATG GGAAAGTGAG TGGCCACCTC 
CACGTGGGCC ATACACCCGT GCACTTCACT GATGTGGACC TGCATGCGTA TATCGTGGGC 
AATGATGGCA GAGCCTACAC GGCCATCAGC CACATCCCAC AGCCAGCAGC CCAGGCCCTC 
CTCCCCCTCA CACCAATTGG AGGCCTGTTT GGCTGGCTCT TTGCTTTAGA AAAACCTGGC 
TCTGAGAACG GCTTCAGCCT CGCAGGTGCT GCCTTTACCC ATGACATGGA AGTTACATTC 
TACCCGGGAG AGGAGACGGT TCGTATCACT CAAACTGCTG AGGGACTTGA CCCAGAGAAC 
TACCTGAGCA TTAAGACCAA CATTCAAGGC CAGGTGCCTT ACGTCTCAGC AAATTTCACA 
GCCCACATCT CTCCCTACAA GGAGCTGTAC CACTACTCCG ACTCCACTGT GACCTCTACA 
AGTTCCAGAG ACTACTCTCT GACTTTTGGT GCAATCAACC AAACATGGTC CTACCGCATC 
CACCAGAACA TCACTTACCA GGTGTGCAGG CACGCCCCCA GACACCCGTC CTTCCCCACC 
ACCCAGCAGC TGAACGTGGA CCGGGTCTTT GCCTTGTATA ATGACGAAGA AAGAGTGCTT 
AGATTTGCTG TGACCAATCA AATTGGCCCG GTCAAAGAGG ATTCAGACCC CACTCCGGGG 
AATCCTTGCT ATGATGGGAG CCACATGTGT GACACAACAG CACGGTGCCA TCCAGGGACA 
GGTGTAGATT ACACCTGTGA GTGCGCATCT GGGTACCAGG GAGATGGACG GAACTGTGTG 
GATGAAAATG AATGTGCAAC TGGCTTTCAT CGCTGTGGCC CCAACTCTGT ATGTATCAAC 
TTGCCTGGAA GCTACAGGTG TGAGTGCCGG AGTGGTTATG AGTTTGCAGA TGACCGGCAT 
ACTTGCATCT ATGTAGATGA ATGCTCAGAA AACAGATGTC ACCCTGCAGC TACCTGCTAC 
AATACTCCTG GTTCCTTCTC CTGCCGTTGT CAACCCGGAT ATTATGGGGA TGGATTTCAG 
TGCATACCTG ACTCCACCTC AAGCCTGACA CCCTGTGAAC AACAGCAGCG CCATGCCCAG 
GCCCAGTATG CCTACCCTGG GGCCCGGTTC CACATCCCCC AATGCGACGA GCAGGGCAAC 
TTCCTGCCCC TACAGTGTCA TGGCAGCACT GGTTTCTGCT GGTGCGTGGA CCCTGATGGT 
CATGAAGTTC CTGGTACCCA GACTCCACCT GGCTCCACCC CGCCTCACTG TGGACCATCA 
CCAGAGCCCA CCCAGAGGCC CCCGACCATC TGTGAGCGCT GGAGGGAAAA CCTGCTGGAG 
CACTACGGTG GCACCCCCCG GGATGACCAG TACGTGCCCC AGTGCGATGA CCTGGGCCAC 
TTCATCCCCC TGCAGTGCCA CGGAAAGAGC GACTTCTGCT GGTGTGTGGA CAAAGATGGC 
AGAGAGGTGC AGGGCACCCG CTCCCAGCCA GGCACCACCC CTGCGTGTAT ACCCACCGTC 
GCTCCACCCA TGGTCCGGCC CACGCCCCGG CCAGATGTGA CCCCTCCATC TGTGGGCACC 
TTCCTGCTCT ATACTCAGGG CCAGCAGATT GGCTACTTAC CCCTCAATGG CACCAGGCTT 
CAGAAGGATG CAGCTAAGAC CCTGCTGTCT CTGCATGGCT CCATAATCGT GGGAATTGAT 
TACGACTGCC GGGAGAGGAT GGTGTACTGG ACAGATGTTG CTGGACGGAC AATCAGCCGT 
GCTGGTCTGG AACTGGGAGC AGAGCCTGAG ACGATCGTGA ATTCAGGTCT GATAAGCCCT 
GAAGGACTTG CCATAGACCA CATCCGCAGA ACAATGTACT GGACGGACAG TGTCCTGGAT 
AAGATAGAGA GCGCCCTGCT GGATGGCTCT GAGCGCAAGG TCCTCTTCTA CACAGATCTG 
GTGAATCCCC GTGCCATCGC TGTGGATCCA ATCCGAGGCA ACTTGTACTG GACAGACTGG 
AATAGAGAAG CTCCTAAAAT TGAAACGTCA TCTTTAGATG GAGAAAACAG AAGAATTCTG 
ATCAATACAG ACATTGGATT GCCCAATGGC TTAACCTTTG ACCCTTTCTC TAAACTGCTC 
TGCTGGGCAG ATGCAGGAAC CAAAAAACTG GAGTGTACAC TACCTGATGG AACTGGACGG 
CGTGTCATTC AAAACAACCT CAAGTACCCC TTCAGCATCG TAAGCTATGC AGATCACTTC 
TACCACACAG ACTGGAGGAG GGATGGTGTT GTATCAGTAA ATAAACATAG TGGCCAGTTT 
ACTGATGAGT ATCTCCCAGA ACAACGATCT CACCTCTACG GGATAACTGC AGTCTACCCC 
TACTGCCCAA CAGGAAGAAA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005267464.1
CDS	66..4049
Translation	MEGDRVAGRPVLSSLPVLLLLPLLMLRAAALHPDELFPHGESWGDQLLQEGDDESSAVVKLANPLHFYEARFSNLYVGTNGIISTQDFPRETQYVDYDFPTDFPAIAPFLADIDTSHGRGRVLYREDTSPAVLGLAARYVRAGFPRSARFTPTHAFLATWEQVGAYEEVKRGALPSGELNTFQAVLASDGSDSYALFLYPANGLQFLGTRPKESYNVQLQLPARVGFCRGEADDLKSEGPYFSLTSTEQSVKNLYQLSNLGIPGVWAFHIGSTSPLDNVRPAAVGDLSAAHSSVPLGRSFSHATALESDYNEDNLDYYDVNEEEAEYLPGEPEEALNGHSSIDVSFQSKVDTKPLEESSTLDPHTKEGTSLGEVGGPDLKGQVEPWDERETRSPAPPEVDRDSLAPSWETPPPYPENGSIQPYPDGGPVPSEMDVPPAHPEEEIVLRSYPASGHTTPLSRGTYEVGLEDNIGSNTEVFTYNAANKETCEHNHRQCSRHAFCTDYATGFCCHCQSKFYGNGKHCLPEGAPHRVNGKVSGHLHVGHTPVHFTDVDLHAYIVGNDGRAYTAISHIPQPAAQALLPLTPIGGLFGWLFALEKPGSENGFSLAGAAFTHDMEVTFYPGEETVRITQTAEGLDPENYLSIKTNIQGQVPYVSANFTAHISPYKELYHYSDSTVTSTSSRDYSLTFGAINQTWSYRIHQNITYQVCRHAPRHPSFPTTQQLNVDRVFALYNDEERVLRFAVTNQIGPVKEDSDPTPGNPCYDGSHMCDTTARCHPGTGVDYTCECASGYQGDGRNCVDENECATGFHRCGPNSVCINLPGSYRCECRSGYEFADDRHTCIYVDECSENRCHPAATCYNTPGSFSCRCQPGYYGDGFQCIPDSTSSLTPCEQQQRHAQAQYAYPGARFHIPQCDEQGNFLPLQCHGSTGFCWCVDPDGHEVPGTQTPPGSTPPHCGPSPEPTQRPPTICERWRENLLEHYGGTPRDDQYVPQCDDLGHFIPLQCHGKSDFCWCVDKDGREVQGTRSQPGTTPACIPTVAPPMVRPTPRPDVTPPSVGTFLLYTQGQQIGYLPLNGTRLQKDAAKTLLSLHGSIIVGIDYDCRERMVYWTDVAGRTISRAGLELGAEPETIVNSGLISPEGLAIDHIRRTMYWTDSVLDKIESALLDGSERKVLFYTDLVNPRAIAVDPIRGNLYWTDWNREAPKIETSSLDGENRRILINTDIGLPNGLTFDPFSKLLCWADAGTKKLECTLPDGTGRRVIQNNLKYPFSIVSYADHFYHTDWRRDGVVSVNKHSGQFTDEYLPEQRSHLYGITAVYPYCPTGRK

Target ORF information:

RefSeq Version	XM_005267407.5
Organism	Homo sapiens(human)
Definition	Homo sapiens nidogen 2 (NID2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005267407.5

ORF Insert Sequence:

ATGGAGGGGG ACCGGGTGGC CGGGCGGCCG GTGCTGTCGT CGTTACCAGT GCTACTGCTG 
CTGCCGTTGC TAATGTTGCG GGCCGCGGCG CTGCACCCAG ACGAGCTCTT CCCACACGGG 
GAGTCGTGGG GGGACCAGCT CCTGCAGGAA GGCGACGACG AAAGCTCAGC CGTGGTGAAG 
CTGGCGAATC CCCTGCACTT CTACGAAGCC CGATTCAGCA ACCTCTACGT GGGCACCAAC 
GGCATCATCT CCACTCAGGA CTTCCCCAGG GAAACGCAGT ATGTGGACTA TGATTTCCCC 
ACCGACTTCC CGGCCATCGC CCCTTTTCTG GCGGACATCG ACACGAGCCA CGGCAGAGGC 
CGAGTCCTGT ACCGAGAGGA CACCTCCCCC GCAGTGCTGG GCCTGGCCGC CCGCTATGTG 
CGCGCTGGCT TCCCGCGCTC TGCGCGCTTT ACCCCCACCC ACGCCTTCCT GGCCACCTGG 
GAGCAGGTAG GCGCTTACGA GGAGGTCAAG CGCGGGGCGC TGCCCTCGGG AGAGCTGAAC 
ACTTTCCAGG CAGTTTTGGC ATCTGATGGG TCTGATAGCT ACGCCCTCTT TCTTTATCCT 
GCCAACGGCC TGCAGTTCCT TGGAACCCGC CCCAAAGAGT CTTACAATGT CCAGCTTCAG 
CTTCCAGCTC GGGTGGGCTT CTGCCGAGGG GAGGCTGATG ATCTGAAGTC AGAAGGACCA 
TATTTCAGCT TGACTAGCAC TGAACAGTCT GTGAAAAATC TCTATCAACT AAGCAACCTG 
GGGATCCCTG GAGTGTGGGC TTTCCATATC GGCAGCACTT CCCCGTTGGA CAATGTCAGG 
CCAGCTGCAG TTGGAGACCT TTCCGCTGCC CACTCTTCTG TTCCCCTGGG ACGTTCCTTC 
AGCCATGCTA CAGCCCTGGA AAGTGACTAT AATGAGGACA ATTTGGATTA CTATGATGTG 
AATGAGGAGG AAGCTGAATA CCTTCCGGGT GAACCAGAGG AGGCATTGAA TGGCCACAGC 
AGCATTGATG TTTCCTTCCA ATCCAAAGTG GATACAAAGC CTTTAGAGGA ATCTTCCACC 
TTGGATCCTC ACACCAAAGA AGGAACATCT CTGGGAGAGG TAGGGGGCCC AGATTTAAAA 
GGCCAAGTTG AGCCCTGGGA TGAGAGAGAG ACCAGAAGCC CAGCTCCACC AGAGGTAGAC 
AGAGATTCAC TGGCTCCTTC CTGGGAAACC CCACCACCGT ACCCCGAAAA CGGAAGCATC 
CAGCCCTACC CAGATGGAGG GCCAGTGCCT TCGGAAATGG ATGTTCCCCC AGCTCATCCT 
GAAGAAGAAA TTGTTCTTCG AAGTTACCCT GCTTCAGGTC ACACTACACC CTTAAGTCGA 
GGGACGTATG AGGTGGGACT GGAAGACAAC ATAGGTTCCA ACACCGAGGT CTTCACGTAT 
AATGCTGCCA ACAAGGAAAC CTGTGAACAC AACCACAGAC AATGCTCCCG GCATGCCTTC 
TGCACGGACT ATGCCACTGG CTTCTGCTGC CACTGCCAAT CCAAGTTTTA TGGAAATGGG 
AAGCACTGTC TGCCTGAAGG GGCACCTCAC CGAGTGAATG GGAAAGTGAG TGGCCACCTC 
CACGTGGGCC ATACACCCGT GCACTTCACT GATGTGGACC TGCATGCGTA TATCGTGGGC 
AATGATGGCA GAGCCTACAC GGCCATCAGC CACATCCCAC AGCCAGCAGC CCAGGCCCTC 
CTCCCCCTCA CACCAATTGG AGGCCTGTTT GGCTGGCTCT TTGCTTTAGA AAAACCTGGC 
TCTGAGAACG GCTTCAGCCT CGCAGGTGCT GCCTTTACCC ATGACATGGA AGTTACATTC 
TACCCGGGAG AGGAGACGGT TCGTATCACT CAAACTGCTG AGGGACTTGA CCCAGAGAAC 
TACCTGAGCA TTAAGACCAA CATTCAAGGC CAGGTGCCTT ACGTCTCAGC AAATTTCACA 
GCCCACATCT CTCCCTACAA GGAGCTGTAC CACTACTCCG ACTCCACTGT GACCTCTACA 
AGTTCCAGAG ACTACTCTCT GACTTTTGGT GCAATCAACC AAACATGGTC CTACCGCATC 
CACCAGAACA TCACTTACCA GGTGTGCAGG CACGCCCCCA GACACCCGTC CTTCCCCACC 
ACCCAGCAGC TGAACGTGGA CCGGGTCTTT GCCTTGTATA ATGACGAAGA AAGAGTGCTT 
AGATTTGCTG TGACCAATCA AATTGGCCCG GTCAAAGAGG ATTCAGACCC CACTCCGGGG 
AATCCTTGCT ATGATGGGAG CCACATGTGT GACACAACAG CACGGTGCCA TCCAGGGACA 
GGTGTAGATT ACACCTGTGA GTGCGCATCT GGGTACCAGG GAGATGGACG GAACTGTGTG 
GATGAAAATG AATGTGCAAC TGGCTTTCAT CGCTGTGGCC CCAACTCTGT ATGTATCAAC 
TTGCCTGGAA GCTACAGGTG TGAGTGCCGG AGTGGTTATG AGTTTGCAGA TGACCGGCAT 
ACTTGCATCT ATGTAGATGA ATGCTCAGAA AACAGATGTC ACCCTGCAGC TACCTGCTAC 
AATACTCCTG GTTCCTTCTC CTGCCGTTGT CAACCCGGAT ATTATGGGGA TGGATTTCAG 
TGCATACCTG ACTCCACCTC AAGCCTGACA CCCTGTGAAC AACAGCAGCG CCATGCCCAG 
GCCCAGTATG CCTACCCTGG GGCCCGGTTC CACATCCCCC AATGCGACGA GCAGGGCAAC 
TTCCTGCCCC TACAGTGTCA TGGCAGCACT GGTTTCTGCT GGTGCGTGGA CCCTGATGGT 
CATGAAGTTC CTGGTACCCA GACTCCACCT GGCTCCACCC CGCCTCACTG TGGACCATCA 
CCAGAGCCCA CCCAGAGGCC CCCGACCATC TGTGAGCGCT GGAGGGAAAA CCTGCTGGAG 
CACTACGGTG GCACCCCCCG GGATGACCAG TACGTGCCCC AGTGCGATGA CCTGGGCCAC 
TTCATCCCCC TGCAGTGCCA CGGAAAGAGC GACTTCTGCT GGTGTGTGGA CAAAGATGGC 
AGAGAGGTGC AGGGCACCCG CTCCCAGCCA GGCACCACCC CTGCGTGTAT ACCCACCGTC 
GCTCCACCCA TGGTCCGGCC CACGCCCCGG CCAGATGTGA CCCCTCCATC TGTGGGCACC 
TTCCTGCTCT ATACTCAGGG CCAGCAGATT GGCTACTTAC CCCTCAATGG CACCAGGCTT 
CAGAAGGATG CAGCTAAGAC CCTGCTGTCT CTGCATGGCT CCATAATCGT GGGAATTGAT 
TACGACTGCC GGGAGAGGAT GGTGTACTGG ACAGATGTTG CTGGACGGAC AATCAGCCGT 
GCTGGTCTGG AACTGGGAGC AGAGCCTGAG ACGATCGTGA ATTCAGGTCT GATAAGCCCT 
GAAGGACTTG CCATAGACCA CATCCGCAGA ACAATGTACT GGACGGACAG TGTCCTGGAT 
AAGATAGAGA GCGCCCTGCT GGATGGCTCT GAGCGCAAGG TCCTCTTCTA CACAGATCTG 
GTGAATCCCC GTGCCATCGC TGTGGATCCA ATCCGAGGCA ACTTGTACTG GACAGACTGG 
AATAGAGAAG CTCCTAAAAT TGAAACGTCA TCTTTAGATG GAGAAAACAG AAGAATTCTG 
ATCAATACAG ACATTGGATT GCCCAATGGC TTAACCTTTG ACCCTTTCTC TAAACTGCTC 
TGCTGGGCAG ATGCAGGAAC CAAAAAACTG GAGTGTACAC TACCTGATGG AACTGGACGG 
CGTGTCATTC AAAACAACCT CAAGTACCCC TTCAGCATCG TAAGCTATGC AGATCACTTC 
TACCACACAG ACTGGAGGAG GGATGGTGTT GTATCAGTAA ATAAACATAG TGGCCAGTTT 
ACTGATGAGT ATCTCCCAGA ACAACGATCT CACCTCTACG GGATAACTGC AGTCTACCCC 
TACTGCCCAA CAGGAAGAAA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NID2 ( XM_005267407.4 ) cDNA ORF clone, Homo sapiens(human) -> XP_005267464.1 Homo sapiens nidogen 2 (NID2), transcript variant X3, mRNA.

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CloneID	OHu47583
Clone ID Related Accession (Same CDS sequence)	XM_005267407.5 , XM_005267407.4
Accession Version	XM_005267407.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3984bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	nidogen-2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000014.9) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005267407.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGGGG ACCGGGTGGC CGGGCGGCCG GTGCTGTCGT CGTTACCAGT GCTACTGCTG 
CTGCCGTTGC TAATGTTGCG GGCCGCGGCG CTGCACCCAG ACGAGCTCTT CCCACACGGG 
GAGTCGTGGG GGGACCAGCT CCTGCAGGAA GGCGACGACG AAAGCTCAGC CGTGGTGAAG 
CTGGCGAATC CCCTGCACTT CTACGAAGCC CGATTCAGCA ACCTCTACGT GGGCACCAAC 
GGCATCATCT CCACTCAGGA CTTCCCCAGG GAAACGCAGT ATGTGGACTA TGATTTCCCC 
ACCGACTTCC CGGCCATCGC CCCTTTTCTG GCGGACATCG ACACGAGCCA CGGCAGAGGC 
CGAGTCCTGT ACCGAGAGGA CACCTCCCCC GCAGTGCTGG GCCTGGCCGC CCGCTATGTG 
CGCGCTGGCT TCCCGCGCTC TGCGCGCTTT ACCCCCACCC ACGCCTTCCT GGCCACCTGG 
GAGCAGGTAG GCGCTTACGA GGAGGTCAAG CGCGGGGCGC TGCCCTCGGG AGAGCTGAAC 
ACTTTCCAGG CAGTTTTGGC ATCTGATGGG TCTGATAGCT ACGCCCTCTT TCTTTATCCT 
GCCAACGGCC TGCAGTTCCT TGGAACCCGC CCCAAAGAGT CTTACAATGT CCAGCTTCAG 
CTTCCAGCTC GGGTGGGCTT CTGCCGAGGG GAGGCTGATG ATCTGAAGTC AGAAGGACCA 
TATTTCAGCT TGACTAGCAC TGAACAGTCT GTGAAAAATC TCTATCAACT AAGCAACCTG 
GGGATCCCTG GAGTGTGGGC TTTCCATATC GGCAGCACTT CCCCGTTGGA CAATGTCAGG 
CCAGCTGCAG TTGGAGACCT TTCCGCTGCC CACTCTTCTG TTCCCCTGGG ACGTTCCTTC 
AGCCATGCTA CAGCCCTGGA AAGTGACTAT AATGAGGACA ATTTGGATTA CTATGATGTG 
AATGAGGAGG AAGCTGAATA CCTTCCGGGT GAACCAGAGG AGGCATTGAA TGGCCACAGC 
AGCATTGATG TTTCCTTCCA ATCCAAAGTG GATACAAAGC CTTTAGAGGA ATCTTCCACC 
TTGGATCCTC ACACCAAAGA AGGAACATCT CTGGGAGAGG TAGGGGGCCC AGATTTAAAA 
GGCCAAGTTG AGCCCTGGGA TGAGAGAGAG ACCAGAAGCC CAGCTCCACC AGAGGTAGAC 
AGAGATTCAC TGGCTCCTTC CTGGGAAACC CCACCACCGT ACCCCGAAAA CGGAAGCATC 
CAGCCCTACC CAGATGGAGG GCCAGTGCCT TCGGAAATGG ATGTTCCCCC AGCTCATCCT 
GAAGAAGAAA TTGTTCTTCG AAGTTACCCT GCTTCAGGTC ACACTACACC CTTAAGTCGA 
GGGACGTATG AGGTGGGACT GGAAGACAAC ATAGGTTCCA ACACCGAGGT CTTCACGTAT 
AATGCTGCCA ACAAGGAAAC CTGTGAACAC AACCACAGAC AATGCTCCCG GCATGCCTTC 
TGCACGGACT ATGCCACTGG CTTCTGCTGC CACTGCCAAT CCAAGTTTTA TGGAAATGGG 
AAGCACTGTC TGCCTGAAGG GGCACCTCAC CGAGTGAATG GGAAAGTGAG TGGCCACCTC 
CACGTGGGCC ATACACCCGT GCACTTCACT GATGTGGACC TGCATGCGTA TATCGTGGGC 
AATGATGGCA GAGCCTACAC GGCCATCAGC CACATCCCAC AGCCAGCAGC CCAGGCCCTC 
CTCCCCCTCA CACCAATTGG AGGCCTGTTT GGCTGGCTCT TTGCTTTAGA AAAACCTGGC 
TCTGAGAACG GCTTCAGCCT CGCAGGTGCT GCCTTTACCC ATGACATGGA AGTTACATTC 
TACCCGGGAG AGGAGACGGT TCGTATCACT CAAACTGCTG AGGGACTTGA CCCAGAGAAC 
TACCTGAGCA TTAAGACCAA CATTCAAGGC CAGGTGCCTT ACGTCTCAGC AAATTTCACA 
GCCCACATCT CTCCCTACAA GGAGCTGTAC CACTACTCCG ACTCCACTGT GACCTCTACA 
AGTTCCAGAG ACTACTCTCT GACTTTTGGT GCAATCAACC AAACATGGTC CTACCGCATC 
CACCAGAACA TCACTTACCA GGTGTGCAGG CACGCCCCCA GACACCCGTC CTTCCCCACC 
ACCCAGCAGC TGAACGTGGA CCGGGTCTTT GCCTTGTATA ATGACGAAGA AAGAGTGCTT 
AGATTTGCTG TGACCAATCA AATTGGCCCG GTCAAAGAGG ATTCAGACCC CACTCCGGGG 
AATCCTTGCT ATGATGGGAG CCACATGTGT GACACAACAG CACGGTGCCA TCCAGGGACA 
GGTGTAGATT ACACCTGTGA GTGCGCATCT GGGTACCAGG GAGATGGACG GAACTGTGTG 
GATGAAAATG AATGTGCAAC TGGCTTTCAT CGCTGTGGCC CCAACTCTGT ATGTATCAAC 
TTGCCTGGAA GCTACAGGTG TGAGTGCCGG AGTGGTTATG AGTTTGCAGA TGACCGGCAT 
ACTTGCATCT ATGTAGATGA ATGCTCAGAA AACAGATGTC ACCCTGCAGC TACCTGCTAC 
AATACTCCTG GTTCCTTCTC CTGCCGTTGT CAACCCGGAT ATTATGGGGA TGGATTTCAG 
TGCATACCTG ACTCCACCTC AAGCCTGACA CCCTGTGAAC AACAGCAGCG CCATGCCCAG 
GCCCAGTATG CCTACCCTGG GGCCCGGTTC CACATCCCCC AATGCGACGA GCAGGGCAAC 
TTCCTGCCCC TACAGTGTCA TGGCAGCACT GGTTTCTGCT GGTGCGTGGA CCCTGATGGT 
CATGAAGTTC CTGGTACCCA GACTCCACCT GGCTCCACCC CGCCTCACTG TGGACCATCA 
CCAGAGCCCA CCCAGAGGCC CCCGACCATC TGTGAGCGCT GGAGGGAAAA CCTGCTGGAG 
CACTACGGTG GCACCCCCCG GGATGACCAG TACGTGCCCC AGTGCGATGA CCTGGGCCAC 
TTCATCCCCC TGCAGTGCCA CGGAAAGAGC GACTTCTGCT GGTGTGTGGA CAAAGATGGC 
AGAGAGGTGC AGGGCACCCG CTCCCAGCCA GGCACCACCC CTGCGTGTAT ACCCACCGTC 
GCTCCACCCA TGGTCCGGCC CACGCCCCGG CCAGATGTGA CCCCTCCATC TGTGGGCACC 
TTCCTGCTCT ATACTCAGGG CCAGCAGATT GGCTACTTAC CCCTCAATGG CACCAGGCTT 
CAGAAGGATG CAGCTAAGAC CCTGCTGTCT CTGCATGGCT CCATAATCGT GGGAATTGAT 
TACGACTGCC GGGAGAGGAT GGTGTACTGG ACAGATGTTG CTGGACGGAC AATCAGCCGT 
GCTGGTCTGG AACTGGGAGC AGAGCCTGAG ACGATCGTGA ATTCAGGTCT GATAAGCCCT 
GAAGGACTTG CCATAGACCA CATCCGCAGA ACAATGTACT GGACGGACAG TGTCCTGGAT 
AAGATAGAGA GCGCCCTGCT GGATGGCTCT GAGCGCAAGG TCCTCTTCTA CACAGATCTG 
GTGAATCCCC GTGCCATCGC TGTGGATCCA ATCCGAGGCA ACTTGTACTG GACAGACTGG 
AATAGAGAAG CTCCTAAAAT TGAAACGTCA TCTTTAGATG GAGAAAACAG AAGAATTCTG 
ATCAATACAG ACATTGGATT GCCCAATGGC TTAACCTTTG ACCCTTTCTC TAAACTGCTC 
TGCTGGGCAG ATGCAGGAAC CAAAAAACTG GAGTGTACAC TACCTGATGG AACTGGACGG 
CGTGTCATTC AAAACAACCT CAAGTACCCC TTCAGCATCG TAAGCTATGC AGATCACTTC 
TACCACACAG ACTGGAGGAG GGATGGTGTT GTATCAGTAA ATAAACATAG TGGCCAGTTT 
ACTGATGAGT ATCTCCCAGA ACAACGATCT CACCTCTACG GGATAACTGC AGTCTACCCC 
TACTGCCCAA CAGGAAGAAA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005267464.1
CDS	238..4221
Translation	MEGDRVAGRPVLSSLPVLLLLPLLMLRAAALHPDELFPHGESWGDQLLQEGDDESSAVVKLANPLHFYEARFSNLYVGTNGIISTQDFPRETQYVDYDFPTDFPAIAPFLADIDTSHGRGRVLYREDTSPAVLGLAARYVRAGFPRSARFTPTHAFLATWEQVGAYEEVKRGALPSGELNTFQAVLASDGSDSYALFLYPANGLQFLGTRPKESYNVQLQLPARVGFCRGEADDLKSEGPYFSLTSTEQSVKNLYQLSNLGIPGVWAFHIGSTSPLDNVRPAAVGDLSAAHSSVPLGRSFSHATALESDYNEDNLDYYDVNEEEAEYLPGEPEEALNGHSSIDVSFQSKVDTKPLEESSTLDPHTKEGTSLGEVGGPDLKGQVEPWDERETRSPAPPEVDRDSLAPSWETPPPYPENGSIQPYPDGGPVPSEMDVPPAHPEEEIVLRSYPASGHTTPLSRGTYEVGLEDNIGSNTEVFTYNAANKETCEHNHRQCSRHAFCTDYATGFCCHCQSKFYGNGKHCLPEGAPHRVNGKVSGHLHVGHTPVHFTDVDLHAYIVGNDGRAYTAISHIPQPAAQALLPLTPIGGLFGWLFALEKPGSENGFSLAGAAFTHDMEVTFYPGEETVRITQTAEGLDPENYLSIKTNIQGQVPYVSANFTAHISPYKELYHYSDSTVTSTSSRDYSLTFGAINQTWSYRIHQNITYQVCRHAPRHPSFPTTQQLNVDRVFALYNDEERVLRFAVTNQIGPVKEDSDPTPGNPCYDGSHMCDTTARCHPGTGVDYTCECASGYQGDGRNCVDENECATGFHRCGPNSVCINLPGSYRCECRSGYEFADDRHTCIYVDECSENRCHPAATCYNTPGSFSCRCQPGYYGDGFQCIPDSTSSLTPCEQQQRHAQAQYAYPGARFHIPQCDEQGNFLPLQCHGSTGFCWCVDPDGHEVPGTQTPPGSTPPHCGPSPEPTQRPPTICERWRENLLEHYGGTPRDDQYVPQCDDLGHFIPLQCHGKSDFCWCVDKDGREVQGTRSQPGTTPACIPTVAPPMVRPTPRPDVTPPSVGTFLLYTQGQQIGYLPLNGTRLQKDAAKTLLSLHGSIIVGIDYDCRERMVYWTDVAGRTISRAGLELGAEPETIVNSGLISPEGLAIDHIRRTMYWTDSVLDKIESALLDGSERKVLFYTDLVNPRAIAVDPIRGNLYWTDWNREAPKIETSSLDGENRRILINTDIGLPNGLTFDPFSKLLCWADAGTKKLECTLPDGTGRRVIQNNLKYPFSIVSYADHFYHTDWRRDGVVSVNKHSGQFTDEYLPEQRSHLYGITAVYPYCPTGRK

Target ORF information:

RefSeq Version	XM_005267407.4
Organism	Homo sapiens(human)
Definition	Homo sapiens nidogen 2 (NID2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005267407.4

ORF Insert Sequence:

ATGGAGGGGG ACCGGGTGGC CGGGCGGCCG GTGCTGTCGT CGTTACCAGT GCTACTGCTG 
CTGCCGTTGC TAATGTTGCG GGCCGCGGCG CTGCACCCAG ACGAGCTCTT CCCACACGGG 
GAGTCGTGGG GGGACCAGCT CCTGCAGGAA GGCGACGACG AAAGCTCAGC CGTGGTGAAG 
CTGGCGAATC CCCTGCACTT CTACGAAGCC CGATTCAGCA ACCTCTACGT GGGCACCAAC 
GGCATCATCT CCACTCAGGA CTTCCCCAGG GAAACGCAGT ATGTGGACTA TGATTTCCCC 
ACCGACTTCC CGGCCATCGC CCCTTTTCTG GCGGACATCG ACACGAGCCA CGGCAGAGGC 
CGAGTCCTGT ACCGAGAGGA CACCTCCCCC GCAGTGCTGG GCCTGGCCGC CCGCTATGTG 
CGCGCTGGCT TCCCGCGCTC TGCGCGCTTT ACCCCCACCC ACGCCTTCCT GGCCACCTGG 
GAGCAGGTAG GCGCTTACGA GGAGGTCAAG CGCGGGGCGC TGCCCTCGGG AGAGCTGAAC 
ACTTTCCAGG CAGTTTTGGC ATCTGATGGG TCTGATAGCT ACGCCCTCTT TCTTTATCCT 
GCCAACGGCC TGCAGTTCCT TGGAACCCGC CCCAAAGAGT CTTACAATGT CCAGCTTCAG 
CTTCCAGCTC GGGTGGGCTT CTGCCGAGGG GAGGCTGATG ATCTGAAGTC AGAAGGACCA 
TATTTCAGCT TGACTAGCAC TGAACAGTCT GTGAAAAATC TCTATCAACT AAGCAACCTG 
GGGATCCCTG GAGTGTGGGC TTTCCATATC GGCAGCACTT CCCCGTTGGA CAATGTCAGG 
CCAGCTGCAG TTGGAGACCT TTCCGCTGCC CACTCTTCTG TTCCCCTGGG ACGTTCCTTC 
AGCCATGCTA CAGCCCTGGA AAGTGACTAT AATGAGGACA ATTTGGATTA CTATGATGTG 
AATGAGGAGG AAGCTGAATA CCTTCCGGGT GAACCAGAGG AGGCATTGAA TGGCCACAGC 
AGCATTGATG TTTCCTTCCA ATCCAAAGTG GATACAAAGC CTTTAGAGGA ATCTTCCACC 
TTGGATCCTC ACACCAAAGA AGGAACATCT CTGGGAGAGG TAGGGGGCCC AGATTTAAAA 
GGCCAAGTTG AGCCCTGGGA TGAGAGAGAG ACCAGAAGCC CAGCTCCACC AGAGGTAGAC 
AGAGATTCAC TGGCTCCTTC CTGGGAAACC CCACCACCGT ACCCCGAAAA CGGAAGCATC 
CAGCCCTACC CAGATGGAGG GCCAGTGCCT TCGGAAATGG ATGTTCCCCC AGCTCATCCT 
GAAGAAGAAA TTGTTCTTCG AAGTTACCCT GCTTCAGGTC ACACTACACC CTTAAGTCGA 
GGGACGTATG AGGTGGGACT GGAAGACAAC ATAGGTTCCA ACACCGAGGT CTTCACGTAT 
AATGCTGCCA ACAAGGAAAC CTGTGAACAC AACCACAGAC AATGCTCCCG GCATGCCTTC 
TGCACGGACT ATGCCACTGG CTTCTGCTGC CACTGCCAAT CCAAGTTTTA TGGAAATGGG 
AAGCACTGTC TGCCTGAAGG GGCACCTCAC CGAGTGAATG GGAAAGTGAG TGGCCACCTC 
CACGTGGGCC ATACACCCGT GCACTTCACT GATGTGGACC TGCATGCGTA TATCGTGGGC 
AATGATGGCA GAGCCTACAC GGCCATCAGC CACATCCCAC AGCCAGCAGC CCAGGCCCTC 
CTCCCCCTCA CACCAATTGG AGGCCTGTTT GGCTGGCTCT TTGCTTTAGA AAAACCTGGC 
TCTGAGAACG GCTTCAGCCT CGCAGGTGCT GCCTTTACCC ATGACATGGA AGTTACATTC 
TACCCGGGAG AGGAGACGGT TCGTATCACT CAAACTGCTG AGGGACTTGA CCCAGAGAAC 
TACCTGAGCA TTAAGACCAA CATTCAAGGC CAGGTGCCTT ACGTCTCAGC AAATTTCACA 
GCCCACATCT CTCCCTACAA GGAGCTGTAC CACTACTCCG ACTCCACTGT GACCTCTACA 
AGTTCCAGAG ACTACTCTCT GACTTTTGGT GCAATCAACC AAACATGGTC CTACCGCATC 
CACCAGAACA TCACTTACCA GGTGTGCAGG CACGCCCCCA GACACCCGTC CTTCCCCACC 
ACCCAGCAGC TGAACGTGGA CCGGGTCTTT GCCTTGTATA ATGACGAAGA AAGAGTGCTT 
AGATTTGCTG TGACCAATCA AATTGGCCCG GTCAAAGAGG ATTCAGACCC CACTCCGGGG 
AATCCTTGCT ATGATGGGAG CCACATGTGT GACACAACAG CACGGTGCCA TCCAGGGACA 
GGTGTAGATT ACACCTGTGA GTGCGCATCT GGGTACCAGG GAGATGGACG GAACTGTGTG 
GATGAAAATG AATGTGCAAC TGGCTTTCAT CGCTGTGGCC CCAACTCTGT ATGTATCAAC 
TTGCCTGGAA GCTACAGGTG TGAGTGCCGG AGTGGTTATG AGTTTGCAGA TGACCGGCAT 
ACTTGCATCT ATGTAGATGA ATGCTCAGAA AACAGATGTC ACCCTGCAGC TACCTGCTAC 
AATACTCCTG GTTCCTTCTC CTGCCGTTGT CAACCCGGAT ATTATGGGGA TGGATTTCAG 
TGCATACCTG ACTCCACCTC AAGCCTGACA CCCTGTGAAC AACAGCAGCG CCATGCCCAG 
GCCCAGTATG CCTACCCTGG GGCCCGGTTC CACATCCCCC AATGCGACGA GCAGGGCAAC 
TTCCTGCCCC TACAGTGTCA TGGCAGCACT GGTTTCTGCT GGTGCGTGGA CCCTGATGGT 
CATGAAGTTC CTGGTACCCA GACTCCACCT GGCTCCACCC CGCCTCACTG TGGACCATCA 
CCAGAGCCCA CCCAGAGGCC CCCGACCATC TGTGAGCGCT GGAGGGAAAA CCTGCTGGAG 
CACTACGGTG GCACCCCCCG GGATGACCAG TACGTGCCCC AGTGCGATGA CCTGGGCCAC 
TTCATCCCCC TGCAGTGCCA CGGAAAGAGC GACTTCTGCT GGTGTGTGGA CAAAGATGGC 
AGAGAGGTGC AGGGCACCCG CTCCCAGCCA GGCACCACCC CTGCGTGTAT ACCCACCGTC 
GCTCCACCCA TGGTCCGGCC CACGCCCCGG CCAGATGTGA CCCCTCCATC TGTGGGCACC 
TTCCTGCTCT ATACTCAGGG CCAGCAGATT GGCTACTTAC CCCTCAATGG CACCAGGCTT 
CAGAAGGATG CAGCTAAGAC CCTGCTGTCT CTGCATGGCT CCATAATCGT GGGAATTGAT 
TACGACTGCC GGGAGAGGAT GGTGTACTGG ACAGATGTTG CTGGACGGAC AATCAGCCGT 
GCTGGTCTGG AACTGGGAGC AGAGCCTGAG ACGATCGTGA ATTCAGGTCT GATAAGCCCT 
GAAGGACTTG CCATAGACCA CATCCGCAGA ACAATGTACT GGACGGACAG TGTCCTGGAT 
AAGATAGAGA GCGCCCTGCT GGATGGCTCT GAGCGCAAGG TCCTCTTCTA CACAGATCTG 
GTGAATCCCC GTGCCATCGC TGTGGATCCA ATCCGAGGCA ACTTGTACTG GACAGACTGG 
AATAGAGAAG CTCCTAAAAT TGAAACGTCA TCTTTAGATG GAGAAAACAG AAGAATTCTG 
ATCAATACAG ACATTGGATT GCCCAATGGC TTAACCTTTG ACCCTTTCTC TAAACTGCTC 
TGCTGGGCAG ATGCAGGAAC CAAAAAACTG GAGTGTACAC TACCTGATGG AACTGGACGG 
CGTGTCATTC AAAACAACCT CAAGTACCCC TTCAGCATCG TAAGCTATGC AGATCACTTC 
TACCACACAG ACTGGAGGAG GGATGGTGTT GTATCAGTAA ATAAACATAG TGGCCAGTTT 
ACTGATGAGT ATCTCCCAGA ACAACGATCT CACCTCTACG GGATAACTGC AGTCTACCCC 
TACTGCCCAA CAGGAAGAAA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NID2 ( XM_005267405.4 ) cDNA ORF clone, Homo sapiens(human) -> XP_005267462.1 Homo sapiens nidogen 2 (NID2), transcript variant X1, mRNA.

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CloneID	OHu47580
Clone ID Related Accession (Same CDS sequence)	XM_005267405.4 , XM_005267405.5
Accession Version	XM_005267405.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4209bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	nidogen-2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000014.9) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005267405.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGGGG ACCGGGTGGC CGGGCGGCCG GTGCTGTCGT CGTTACCAGT GCTACTGCTG 
CTGCCGTTGC TAATGTTGCG GGCCGCGGCG CTGCACCCAG ACGAGCTCTT CCCACACGGG 
GAGTCGTGGG GGGACCAGCT CCTGCAGGAA GGCGACGACG AAAGCTCAGC CGTGGTGAAG 
CTGGCGAATC CCCTGCACTT CTACGAAGCC CGATTCAGCA ACCTCTACGT GGGCACCAAC 
GGCATCATCT CCACTCAGGA CTTCCCCAGG GAAACGCAGT ATGTGGACTA TGATTTCCCC 
ACCGACTTCC CGGCCATCGC CCCTTTTCTG GCGGACATCG ACACGAGCCA CGGCAGAGGC 
CGAGTCCTGT ACCGAGAGGA CACCTCCCCC GCAGTGCTGG GCCTGGCCGC CCGCTATGTG 
CGCGCTGGCT TCCCGCGCTC TGCGCGCTTT ACCCCCACCC ACGCCTTCCT GGCCACCTGG 
GAGCAGGTAG GCGCTTACGA GGAGGTCAAG CGCGGGGCGC TGCCCTCGGG AGAGCTGAAC 
ACTTTCCAGG CAGTTTTGGC ATCTGATGGG TCTGATAGCT ACGCCCTCTT TCTTTATCCT 
GCCAACGGCC TGCAGTTCCT TGGAACCCGC CCCAAAGAGT CTTACAATGT CCAGCTTCAG 
CTTCCAGCTC GGGTGGGCTT CTGCCGAGGG GAGGCTGATG ATCTGAAGTC AGAAGGACCA 
TATTTCAGCT TGACTAGCAC TGAACAGTCT GTGAAAAATC TCTATCAACT AAGCAACCTG 
GGGATCCCTG GAGTGTGGGC TTTCCATATC GGCAGCACTT CCCCGTTGGA CAATGTCAGG 
CCAGCTGCAG TTGGAGACCT TTCCGCTGCC CACTCTTCTG TTCCCCTGGG ACGTTCCTTC 
AGCCATGCTA CAGCCCTGGA AAGTGACTAT AATGAGGACA ATTTGGATTA CTATGATGTG 
AATGAGGAGG AAGCTGAATA CCTTCCGGGT GAACCAGAGG AGGCATTGAA TGGCCACAGC 
AGCATTGATG TTTCCTTCCA ATCCAAAGTG GATACAAAGC CTTTAGAGGG TAGGATCTCC 
CCTCCAGATT CTGATCTGTC CTCCCCCTTG CATCCAACAC CTACTTATTG GCCATTCTAT 
CCTGAAACAG AATCTTCCAC CTTGGATCCT CACACCAAAG AAGGAACATC TCTGGGAGAG 
GTAGGGGGCC CAGATTTAAA AGGCCAAGTT GAGCCCTGGG ATGAGAGAGA GACCAGAAGC 
CCAGCTCCAC CAGAGGTAGA CAGAGATTCA CTGGCTCCTT CCTGGGAAAC CCCACCACCG 
TACCCCGAAA ACGGAAGCAT CCAGCCCTAC CCAGATGGAG GGCCAGTGCC TTCGGAAATG 
GATGTTCCCC CAGCTCATCC TGAAGAAGAA ATTGTTCTTC GAAGTTACCC TGCTTCAGGT 
CACACTACAC CCTTAAGTCG AGGGACGTAT GAGGTGGGAC TGGAAGACAA CATAGGTTCC 
AACACCGAGG TCTTCACGTA TAATGCTGCC AACAAGGAAA CCTGTGAACA CAACCACAGA 
CAATGCTCCC GGCATGCCTT CTGCACGGAC TATGCCACTG GCTTCTGCTG CCACTGCCAA 
TCCAAGTTTT ATGGAAATGG GAAGCACTGT CTGCCTGAAG GGGCACCTCA CCGAGTGAAT 
GGGAAAGTGA GTGGCCACCT CCACGTGGGC CATACACCCG TGCACTTCAC TGATGTGGAC 
CTGCATGCGT ATATCGTGGG CAATGATGGC AGAGCCTACA CGGCCATCAG CCACATCCCA 
CAGCCAGCAG CCCAGGCCCT CCTCCCCCTC ACACCAATTG GAGGCCTGTT TGGCTGGCTC 
TTTGCTTTAG AAAAACCTGG CTCTGAGAAC GGCTTCAGCC TCGCAGGTGC TGCCTTTACC 
CATGACATGG AAGTTACATT CTACCCGGGA GAGGAGACGG TTCGTATCAC TCAAACTGCT 
GAGGGACTTG ACCCAGAGAA CTACCTGAGC ATTAAGACCA ACATTCAAGG CCAGGTGCCT 
TACGTCTCAG CAAATTTCAC AGCCCACATC TCTCCCTACA AGGAGCTGTA CCACTACTCC 
GACTCCACTG TGACCTCTAC AAGTTCCAGA GACTACTCTC TGACTTTTGG TGCAATCAAC 
CAAACATGGT CCTACCGCAT CCACCAGAAC ATCACTTACC AGGTGTGCAG GCACGCCCCC 
AGACACCCGT CCTTCCCCAC CACCCAGCAG CTGAACGTGG ACCGGGTCTT TGCCTTGTAT 
AATGACGAAG AAAGAGTGCT TAGATTTGCT GTGACCAATC AAATTGGCCC GGTCAAAGAG 
GATTCAGACC CCACTCCGGG GAATCCTTGC TATGATGGGA GCCACATGTG TGACACAACA 
GCACGGTGCC ATCCAGGGAC AGGTGTAGAT TACACCTGTG AGTGCGCATC TGGGTACCAG 
GGAGATGGAC GGAACTGTGT GGATGAAAAT GAATGTGCAA CTGGCTTTCA TCGCTGTGGC 
CCCAACTCTG TATGTATCAA CTTGCCTGGA AGCTACAGGT GTGAGTGCCG GAGTGGTTAT 
GAGTTTGCAG ATGACCGGCA TACTTGCATC TTGATCACCC CACCTGCCAA CCCCTGTGAG 
GATGGCAGTC ATACCTGTGC TCCTGCTGGG CAGGCCCGGT GTGTTCACCA TGGAGGCAGC 
ACGTTCAGCT GTGCCTGCCT GCCTGGTTAT GCCGGCGATG GGCACCAGTG CACTGATGTA 
GATGAATGCT CAGAAAACAG ATGTCACCCT GCAGCTACCT GCTACAATAC TCCTGGTTCC 
TTCTCCTGCC GTTGTCAACC CGGATATTAT GGGGATGGAT TTCAGTGCAT ACCTGACTCC 
ACCTCAAGCC TGACACCCTG TGAACAACAG CAGCGCCATG CCCAGGCCCA GTATGCCTAC 
CCTGGGGCCC GGTTCCACAT CCCCCAATGC GACGAGCAGG GCAACTTCCT GCCCCTACAG 
TGTCATGGCA GCACTGGTTT CTGCTGGTGC GTGGACCCTG ATGGTCATGA AGTTCCTGGT 
ACCCAGACTC CACCTGGCTC CACCCCGCCT CACTGTGGAC CATCACCAGA GCCCACCCAG 
AGGCCCCCGA CCATCTGTGA GCGCTGGAGG GAAAACCTGC TGGAGCACTA CGGTGGCACC 
CCCCGGGATG ACCAGTACGT GCCCCAGTGC GATGACCTGG GCCACTTCAT CCCCCTGCAG 
TGCCACGGAA AGAGCGACTT CTGCTGGTGT GTGGACAAAG ATGGCAGAGA GGTGCAGGGC 
ACCCGCTCCC AGCCAGGCAC CACCCCTGCG TGTATACCCA CCGTCGCTCC ACCCATGGTC 
CGGCCCACGC CCCGGCCAGA TGTGACCCCT CCATCTGTGG GCACCTTCCT GCTCTATACT 
CAGGGCCAGC AGATTGGCTA CTTACCCCTC AATGGCACCA GGCTTCAGAA GGATGCAGCT 
AAGACCCTGC TGTCTCTGCA TGGCTCCATA ATCGTGGGAA TTGATTACGA CTGCCGGGAG 
AGGATGGTGT ACTGGACAGA TGTTGCTGGA CGGACAATCA GCCGTGCTGG TCTGGAACTG 
GGAGCAGAGC CTGAGACGAT CGTGAATTCA GGTCTGATAA GCCCTGAAGG ACTTGCCATA 
GACCACATCC GCAGAACAAT GTACTGGACG GACAGTGTCC TGGATAAGAT AGAGAGCGCC 
CTGCTGGATG GCTCTGAGCG CAAGGTCCTC TTCTACACAG ATCTGGTGAA TCCCCGTGCC 
ATCGCTGTGG ATCCAATCCG AGGCAACTTG TACTGGACAG ACTGGAATAG AGAAGCTCCT 
AAAATTGAAA CGTCATCTTT AGATGGAGAA AACAGAAGAA TTCTGATCAA TACAGACATT 
GGATTGCCCA ATGGCTTAAC CTTTGACCCT TTCTCTAAAC TGCTCTGCTG GGCAGATGCA 
GGAACCAAAA AACTGGAGTG TACACTACCT GATGGAACTG GACGGCGTGT CATTCAAAAC 
AACCTCAAGT ACCCCTTCAG CATCGTAAGC TATGCAGATC ACTTCTACCA CACAGACTGG 
AGGAGGGATG GTGTTGTATC AGTAAATAAA CATAGTGGCC AGTTTACTGA TGAGTATCTC 
CCAGAACAAC GATCTCACCT CTACGGGATA ACTGCAGTCT ACCCCTACTG CCCAACAGGA 
AGAAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005267462.1
CDS	238..4446
Translation	MEGDRVAGRPVLSSLPVLLLLPLLMLRAAALHPDELFPHGESWGDQLLQEGDDESSAVVKLANPLHFYEARFSNLYVGTNGIISTQDFPRETQYVDYDFPTDFPAIAPFLADIDTSHGRGRVLYREDTSPAVLGLAARYVRAGFPRSARFTPTHAFLATWEQVGAYEEVKRGALPSGELNTFQAVLASDGSDSYALFLYPANGLQFLGTRPKESYNVQLQLPARVGFCRGEADDLKSEGPYFSLTSTEQSVKNLYQLSNLGIPGVWAFHIGSTSPLDNVRPAAVGDLSAAHSSVPLGRSFSHATALESDYNEDNLDYYDVNEEEAEYLPGEPEEALNGHSSIDVSFQSKVDTKPLEGRISPPDSDLSSPLHPTPTYWPFYPETESSTLDPHTKEGTSLGEVGGPDLKGQVEPWDERETRSPAPPEVDRDSLAPSWETPPPYPENGSIQPYPDGGPVPSEMDVPPAHPEEEIVLRSYPASGHTTPLSRGTYEVGLEDNIGSNTEVFTYNAANKETCEHNHRQCSRHAFCTDYATGFCCHCQSKFYGNGKHCLPEGAPHRVNGKVSGHLHVGHTPVHFTDVDLHAYIVGNDGRAYTAISHIPQPAAQALLPLTPIGGLFGWLFALEKPGSENGFSLAGAAFTHDMEVTFYPGEETVRITQTAEGLDPENYLSIKTNIQGQVPYVSANFTAHISPYKELYHYSDSTVTSTSSRDYSLTFGAINQTWSYRIHQNITYQVCRHAPRHPSFPTTQQLNVDRVFALYNDEERVLRFAVTNQIGPVKEDSDPTPGNPCYDGSHMCDTTARCHPGTGVDYTCECASGYQGDGRNCVDENECATGFHRCGPNSVCINLPGSYRCECRSGYEFADDRHTCILITPPANPCEDGSHTCAPAGQARCVHHGGSTFSCACLPGYAGDGHQCTDVDECSENRCHPAATCYNTPGSFSCRCQPGYYGDGFQCIPDSTSSLTPCEQQQRHAQAQYAYPGARFHIPQCDEQGNFLPLQCHGSTGFCWCVDPDGHEVPGTQTPPGSTPPHCGPSPEPTQRPPTICERWRENLLEHYGGTPRDDQYVPQCDDLGHFIPLQCHGKSDFCWCVDKDGREVQGTRSQPGTTPACIPTVAPPMVRPTPRPDVTPPSVGTFLLYTQGQQIGYLPLNGTRLQKDAAKTLLSLHGSIIVGIDYDCRERMVYWTDVAGRTISRAGLELGAEPETIVNSGLISPEGLAIDHIRRTMYWTDSVLDKIESALLDGSERKVLFYTDLVNPRAIAVDPIRGNLYWTDWNREAPKIETSSLDGENRRILINTDIGLPNGLTFDPFSKLLCWADAGTKKLECTLPDGTGRRVIQNNLKYPFSIVSYADHFYHTDWRRDGVVSVNKHSGQFTDEYLPEQRSHLYGITAVYPYCPTGRK

Target ORF information:

RefSeq Version	XM_005267405.4
Organism	Homo sapiens(human)
Definition	Homo sapiens nidogen 2 (NID2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005267405.4

ORF Insert Sequence:

ATGGAGGGGG ACCGGGTGGC CGGGCGGCCG GTGCTGTCGT CGTTACCAGT GCTACTGCTG 
CTGCCGTTGC TAATGTTGCG GGCCGCGGCG CTGCACCCAG ACGAGCTCTT CCCACACGGG 
GAGTCGTGGG GGGACCAGCT CCTGCAGGAA GGCGACGACG AAAGCTCAGC CGTGGTGAAG 
CTGGCGAATC CCCTGCACTT CTACGAAGCC CGATTCAGCA ACCTCTACGT GGGCACCAAC 
GGCATCATCT CCACTCAGGA CTTCCCCAGG GAAACGCAGT ATGTGGACTA TGATTTCCCC 
ACCGACTTCC CGGCCATCGC CCCTTTTCTG GCGGACATCG ACACGAGCCA CGGCAGAGGC 
CGAGTCCTGT ACCGAGAGGA CACCTCCCCC GCAGTGCTGG GCCTGGCCGC CCGCTATGTG 
CGCGCTGGCT TCCCGCGCTC TGCGCGCTTT ACCCCCACCC ACGCCTTCCT GGCCACCTGG 
GAGCAGGTAG GCGCTTACGA GGAGGTCAAG CGCGGGGCGC TGCCCTCGGG AGAGCTGAAC 
ACTTTCCAGG CAGTTTTGGC ATCTGATGGG TCTGATAGCT ACGCCCTCTT TCTTTATCCT 
GCCAACGGCC TGCAGTTCCT TGGAACCCGC CCCAAAGAGT CTTACAATGT CCAGCTTCAG 
CTTCCAGCTC GGGTGGGCTT CTGCCGAGGG GAGGCTGATG ATCTGAAGTC AGAAGGACCA 
TATTTCAGCT TGACTAGCAC TGAACAGTCT GTGAAAAATC TCTATCAACT AAGCAACCTG 
GGGATCCCTG GAGTGTGGGC TTTCCATATC GGCAGCACTT CCCCGTTGGA CAATGTCAGG 
CCAGCTGCAG TTGGAGACCT TTCCGCTGCC CACTCTTCTG TTCCCCTGGG ACGTTCCTTC 
AGCCATGCTA CAGCCCTGGA AAGTGACTAT AATGAGGACA ATTTGGATTA CTATGATGTG 
AATGAGGAGG AAGCTGAATA CCTTCCGGGT GAACCAGAGG AGGCATTGAA TGGCCACAGC 
AGCATTGATG TTTCCTTCCA ATCCAAAGTG GATACAAAGC CTTTAGAGGG TAGGATCTCC 
CCTCCAGATT CTGATCTGTC CTCCCCCTTG CATCCAACAC CTACTTATTG GCCATTCTAT 
CCTGAAACAG AATCTTCCAC CTTGGATCCT CACACCAAAG AAGGAACATC TCTGGGAGAG 
GTAGGGGGCC CAGATTTAAA AGGCCAAGTT GAGCCCTGGG ATGAGAGAGA GACCAGAAGC 
CCAGCTCCAC CAGAGGTAGA CAGAGATTCA CTGGCTCCTT CCTGGGAAAC CCCACCACCG 
TACCCCGAAA ACGGAAGCAT CCAGCCCTAC CCAGATGGAG GGCCAGTGCC TTCGGAAATG 
GATGTTCCCC CAGCTCATCC TGAAGAAGAA ATTGTTCTTC GAAGTTACCC TGCTTCAGGT 
CACACTACAC CCTTAAGTCG AGGGACGTAT GAGGTGGGAC TGGAAGACAA CATAGGTTCC 
AACACCGAGG TCTTCACGTA TAATGCTGCC AACAAGGAAA CCTGTGAACA CAACCACAGA 
CAATGCTCCC GGCATGCCTT CTGCACGGAC TATGCCACTG GCTTCTGCTG CCACTGCCAA 
TCCAAGTTTT ATGGAAATGG GAAGCACTGT CTGCCTGAAG GGGCACCTCA CCGAGTGAAT 
GGGAAAGTGA GTGGCCACCT CCACGTGGGC CATACACCCG TGCACTTCAC TGATGTGGAC 
CTGCATGCGT ATATCGTGGG CAATGATGGC AGAGCCTACA CGGCCATCAG CCACATCCCA 
CAGCCAGCAG CCCAGGCCCT CCTCCCCCTC ACACCAATTG GAGGCCTGTT TGGCTGGCTC 
TTTGCTTTAG AAAAACCTGG CTCTGAGAAC GGCTTCAGCC TCGCAGGTGC TGCCTTTACC 
CATGACATGG AAGTTACATT CTACCCGGGA GAGGAGACGG TTCGTATCAC TCAAACTGCT 
GAGGGACTTG ACCCAGAGAA CTACCTGAGC ATTAAGACCA ACATTCAAGG CCAGGTGCCT 
TACGTCTCAG CAAATTTCAC AGCCCACATC TCTCCCTACA AGGAGCTGTA CCACTACTCC 
GACTCCACTG TGACCTCTAC AAGTTCCAGA GACTACTCTC TGACTTTTGG TGCAATCAAC 
CAAACATGGT CCTACCGCAT CCACCAGAAC ATCACTTACC AGGTGTGCAG GCACGCCCCC 
AGACACCCGT CCTTCCCCAC CACCCAGCAG CTGAACGTGG ACCGGGTCTT TGCCTTGTAT 
AATGACGAAG AAAGAGTGCT TAGATTTGCT GTGACCAATC AAATTGGCCC GGTCAAAGAG 
GATTCAGACC CCACTCCGGG GAATCCTTGC TATGATGGGA GCCACATGTG TGACACAACA 
GCACGGTGCC ATCCAGGGAC AGGTGTAGAT TACACCTGTG AGTGCGCATC TGGGTACCAG 
GGAGATGGAC GGAACTGTGT GGATGAAAAT GAATGTGCAA CTGGCTTTCA TCGCTGTGGC 
CCCAACTCTG TATGTATCAA CTTGCCTGGA AGCTACAGGT GTGAGTGCCG GAGTGGTTAT 
GAGTTTGCAG ATGACCGGCA TACTTGCATC TTGATCACCC CACCTGCCAA CCCCTGTGAG 
GATGGCAGTC ATACCTGTGC TCCTGCTGGG CAGGCCCGGT GTGTTCACCA TGGAGGCAGC 
ACGTTCAGCT GTGCCTGCCT GCCTGGTTAT GCCGGCGATG GGCACCAGTG CACTGATGTA 
GATGAATGCT CAGAAAACAG ATGTCACCCT GCAGCTACCT GCTACAATAC TCCTGGTTCC 
TTCTCCTGCC GTTGTCAACC CGGATATTAT GGGGATGGAT TTCAGTGCAT ACCTGACTCC 
ACCTCAAGCC TGACACCCTG TGAACAACAG CAGCGCCATG CCCAGGCCCA GTATGCCTAC 
CCTGGGGCCC GGTTCCACAT CCCCCAATGC GACGAGCAGG GCAACTTCCT GCCCCTACAG 
TGTCATGGCA GCACTGGTTT CTGCTGGTGC GTGGACCCTG ATGGTCATGA AGTTCCTGGT 
ACCCAGACTC CACCTGGCTC CACCCCGCCT CACTGTGGAC CATCACCAGA GCCCACCCAG 
AGGCCCCCGA CCATCTGTGA GCGCTGGAGG GAAAACCTGC TGGAGCACTA CGGTGGCACC 
CCCCGGGATG ACCAGTACGT GCCCCAGTGC GATGACCTGG GCCACTTCAT CCCCCTGCAG 
TGCCACGGAA AGAGCGACTT CTGCTGGTGT GTGGACAAAG ATGGCAGAGA GGTGCAGGGC 
ACCCGCTCCC AGCCAGGCAC CACCCCTGCG TGTATACCCA CCGTCGCTCC ACCCATGGTC 
CGGCCCACGC CCCGGCCAGA TGTGACCCCT CCATCTGTGG GCACCTTCCT GCTCTATACT 
CAGGGCCAGC AGATTGGCTA CTTACCCCTC AATGGCACCA GGCTTCAGAA GGATGCAGCT 
AAGACCCTGC TGTCTCTGCA TGGCTCCATA ATCGTGGGAA TTGATTACGA CTGCCGGGAG 
AGGATGGTGT ACTGGACAGA TGTTGCTGGA CGGACAATCA GCCGTGCTGG TCTGGAACTG 
GGAGCAGAGC CTGAGACGAT CGTGAATTCA GGTCTGATAA GCCCTGAAGG ACTTGCCATA 
GACCACATCC GCAGAACAAT GTACTGGACG GACAGTGTCC TGGATAAGAT AGAGAGCGCC 
CTGCTGGATG GCTCTGAGCG CAAGGTCCTC TTCTACACAG ATCTGGTGAA TCCCCGTGCC 
ATCGCTGTGG ATCCAATCCG AGGCAACTTG TACTGGACAG ACTGGAATAG AGAAGCTCCT 
AAAATTGAAA CGTCATCTTT AGATGGAGAA AACAGAAGAA TTCTGATCAA TACAGACATT 
GGATTGCCCA ATGGCTTAAC CTTTGACCCT TTCTCTAAAC TGCTCTGCTG GGCAGATGCA 
GGAACCAAAA AACTGGAGTG TACACTACCT GATGGAACTG GACGGCGTGT CATTCAAAAC 
AACCTCAAGT ACCCCTTCAG CATCGTAAGC TATGCAGATC ACTTCTACCA CACAGACTGG 
AGGAGGGATG GTGTTGTATC AGTAAATAAA CATAGTGGCC AGTTTACTGA TGAGTATCTC 
CCAGAACAAC GATCTCACCT CTACGGGATA ACTGCAGTCT ACCCCTACTG CCCAACAGGA 
AGAAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NID2 ( XM_005267405.5 ) cDNA ORF clone, Homo sapiens(human) -> XP_005267462.1 Homo sapiens nidogen 2 (NID2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu47580
Clone ID Related Accession (Same CDS sequence)	XM_005267405.4 , XM_005267405.5
Accession Version	XM_005267405.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4209bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	nidogen-2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000014.9) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_005267405.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGGGG ACCGGGTGGC CGGGCGGCCG GTGCTGTCGT CGTTACCAGT GCTACTGCTG 
CTGCCGTTGC TAATGTTGCG GGCCGCGGCG CTGCACCCAG ACGAGCTCTT CCCACACGGG 
GAGTCGTGGG GGGACCAGCT CCTGCAGGAA GGCGACGACG AAAGCTCAGC CGTGGTGAAG 
CTGGCGAATC CCCTGCACTT CTACGAAGCC CGATTCAGCA ACCTCTACGT GGGCACCAAC 
GGCATCATCT CCACTCAGGA CTTCCCCAGG GAAACGCAGT ATGTGGACTA TGATTTCCCC 
ACCGACTTCC CGGCCATCGC CCCTTTTCTG GCGGACATCG ACACGAGCCA CGGCAGAGGC 
CGAGTCCTGT ACCGAGAGGA CACCTCCCCC GCAGTGCTGG GCCTGGCCGC CCGCTATGTG 
CGCGCTGGCT TCCCGCGCTC TGCGCGCTTT ACCCCCACCC ACGCCTTCCT GGCCACCTGG 
GAGCAGGTAG GCGCTTACGA GGAGGTCAAG CGCGGGGCGC TGCCCTCGGG AGAGCTGAAC 
ACTTTCCAGG CAGTTTTGGC ATCTGATGGG TCTGATAGCT ACGCCCTCTT TCTTTATCCT 
GCCAACGGCC TGCAGTTCCT TGGAACCCGC CCCAAAGAGT CTTACAATGT CCAGCTTCAG 
CTTCCAGCTC GGGTGGGCTT CTGCCGAGGG GAGGCTGATG ATCTGAAGTC AGAAGGACCA 
TATTTCAGCT TGACTAGCAC TGAACAGTCT GTGAAAAATC TCTATCAACT AAGCAACCTG 
GGGATCCCTG GAGTGTGGGC TTTCCATATC GGCAGCACTT CCCCGTTGGA CAATGTCAGG 
CCAGCTGCAG TTGGAGACCT TTCCGCTGCC CACTCTTCTG TTCCCCTGGG ACGTTCCTTC 
AGCCATGCTA CAGCCCTGGA AAGTGACTAT AATGAGGACA ATTTGGATTA CTATGATGTG 
AATGAGGAGG AAGCTGAATA CCTTCCGGGT GAACCAGAGG AGGCATTGAA TGGCCACAGC 
AGCATTGATG TTTCCTTCCA ATCCAAAGTG GATACAAAGC CTTTAGAGGG TAGGATCTCC 
CCTCCAGATT CTGATCTGTC CTCCCCCTTG CATCCAACAC CTACTTATTG GCCATTCTAT 
CCTGAAACAG AATCTTCCAC CTTGGATCCT CACACCAAAG AAGGAACATC TCTGGGAGAG 
GTAGGGGGCC CAGATTTAAA AGGCCAAGTT GAGCCCTGGG ATGAGAGAGA GACCAGAAGC 
CCAGCTCCAC CAGAGGTAGA CAGAGATTCA CTGGCTCCTT CCTGGGAAAC CCCACCACCG 
TACCCCGAAA ACGGAAGCAT CCAGCCCTAC CCAGATGGAG GGCCAGTGCC TTCGGAAATG 
GATGTTCCCC CAGCTCATCC TGAAGAAGAA ATTGTTCTTC GAAGTTACCC TGCTTCAGGT 
CACACTACAC CCTTAAGTCG AGGGACGTAT GAGGTGGGAC TGGAAGACAA CATAGGTTCC 
AACACCGAGG TCTTCACGTA TAATGCTGCC AACAAGGAAA CCTGTGAACA CAACCACAGA 
CAATGCTCCC GGCATGCCTT CTGCACGGAC TATGCCACTG GCTTCTGCTG CCACTGCCAA 
TCCAAGTTTT ATGGAAATGG GAAGCACTGT CTGCCTGAAG GGGCACCTCA CCGAGTGAAT 
GGGAAAGTGA GTGGCCACCT CCACGTGGGC CATACACCCG TGCACTTCAC TGATGTGGAC 
CTGCATGCGT ATATCGTGGG CAATGATGGC AGAGCCTACA CGGCCATCAG CCACATCCCA 
CAGCCAGCAG CCCAGGCCCT CCTCCCCCTC ACACCAATTG GAGGCCTGTT TGGCTGGCTC 
TTTGCTTTAG AAAAACCTGG CTCTGAGAAC GGCTTCAGCC TCGCAGGTGC TGCCTTTACC 
CATGACATGG AAGTTACATT CTACCCGGGA GAGGAGACGG TTCGTATCAC TCAAACTGCT 
GAGGGACTTG ACCCAGAGAA CTACCTGAGC ATTAAGACCA ACATTCAAGG CCAGGTGCCT 
TACGTCTCAG CAAATTTCAC AGCCCACATC TCTCCCTACA AGGAGCTGTA CCACTACTCC 
GACTCCACTG TGACCTCTAC AAGTTCCAGA GACTACTCTC TGACTTTTGG TGCAATCAAC 
CAAACATGGT CCTACCGCAT CCACCAGAAC ATCACTTACC AGGTGTGCAG GCACGCCCCC 
AGACACCCGT CCTTCCCCAC CACCCAGCAG CTGAACGTGG ACCGGGTCTT TGCCTTGTAT 
AATGACGAAG AAAGAGTGCT TAGATTTGCT GTGACCAATC AAATTGGCCC GGTCAAAGAG 
GATTCAGACC CCACTCCGGG GAATCCTTGC TATGATGGGA GCCACATGTG TGACACAACA 
GCACGGTGCC ATCCAGGGAC AGGTGTAGAT TACACCTGTG AGTGCGCATC TGGGTACCAG 
GGAGATGGAC GGAACTGTGT GGATGAAAAT GAATGTGCAA CTGGCTTTCA TCGCTGTGGC 
CCCAACTCTG TATGTATCAA CTTGCCTGGA AGCTACAGGT GTGAGTGCCG GAGTGGTTAT 
GAGTTTGCAG ATGACCGGCA TACTTGCATC TTGATCACCC CACCTGCCAA CCCCTGTGAG 
GATGGCAGTC ATACCTGTGC TCCTGCTGGG CAGGCCCGGT GTGTTCACCA TGGAGGCAGC 
ACGTTCAGCT GTGCCTGCCT GCCTGGTTAT GCCGGCGATG GGCACCAGTG CACTGATGTA 
GATGAATGCT CAGAAAACAG ATGTCACCCT GCAGCTACCT GCTACAATAC TCCTGGTTCC 
TTCTCCTGCC GTTGTCAACC CGGATATTAT GGGGATGGAT TTCAGTGCAT ACCTGACTCC 
ACCTCAAGCC TGACACCCTG TGAACAACAG CAGCGCCATG CCCAGGCCCA GTATGCCTAC 
CCTGGGGCCC GGTTCCACAT CCCCCAATGC GACGAGCAGG GCAACTTCCT GCCCCTACAG 
TGTCATGGCA GCACTGGTTT CTGCTGGTGC GTGGACCCTG ATGGTCATGA AGTTCCTGGT 
ACCCAGACTC CACCTGGCTC CACCCCGCCT CACTGTGGAC CATCACCAGA GCCCACCCAG 
AGGCCCCCGA CCATCTGTGA GCGCTGGAGG GAAAACCTGC TGGAGCACTA CGGTGGCACC 
CCCCGGGATG ACCAGTACGT GCCCCAGTGC GATGACCTGG GCCACTTCAT CCCCCTGCAG 
TGCCACGGAA AGAGCGACTT CTGCTGGTGT GTGGACAAAG ATGGCAGAGA GGTGCAGGGC 
ACCCGCTCCC AGCCAGGCAC CACCCCTGCG TGTATACCCA CCGTCGCTCC ACCCATGGTC 
CGGCCCACGC CCCGGCCAGA TGTGACCCCT CCATCTGTGG GCACCTTCCT GCTCTATACT 
CAGGGCCAGC AGATTGGCTA CTTACCCCTC AATGGCACCA GGCTTCAGAA GGATGCAGCT 
AAGACCCTGC TGTCTCTGCA TGGCTCCATA ATCGTGGGAA TTGATTACGA CTGCCGGGAG 
AGGATGGTGT ACTGGACAGA TGTTGCTGGA CGGACAATCA GCCGTGCTGG TCTGGAACTG 
GGAGCAGAGC CTGAGACGAT CGTGAATTCA GGTCTGATAA GCCCTGAAGG ACTTGCCATA 
GACCACATCC GCAGAACAAT GTACTGGACG GACAGTGTCC TGGATAAGAT AGAGAGCGCC 
CTGCTGGATG GCTCTGAGCG CAAGGTCCTC TTCTACACAG ATCTGGTGAA TCCCCGTGCC 
ATCGCTGTGG ATCCAATCCG AGGCAACTTG TACTGGACAG ACTGGAATAG AGAAGCTCCT 
AAAATTGAAA CGTCATCTTT AGATGGAGAA AACAGAAGAA TTCTGATCAA TACAGACATT 
GGATTGCCCA ATGGCTTAAC CTTTGACCCT TTCTCTAAAC TGCTCTGCTG GGCAGATGCA 
GGAACCAAAA AACTGGAGTG TACACTACCT GATGGAACTG GACGGCGTGT CATTCAAAAC 
AACCTCAAGT ACCCCTTCAG CATCGTAAGC TATGCAGATC ACTTCTACCA CACAGACTGG 
AGGAGGGATG GTGTTGTATC AGTAAATAAA CATAGTGGCC AGTTTACTGA TGAGTATCTC 
CCAGAACAAC GATCTCACCT CTACGGGATA ACTGCAGTCT ACCCCTACTG CCCAACAGGA 
AGAAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005267462.1
CDS	66..4274
Translation	MEGDRVAGRPVLSSLPVLLLLPLLMLRAAALHPDELFPHGESWGDQLLQEGDDESSAVVKLANPLHFYEARFSNLYVGTNGIISTQDFPRETQYVDYDFPTDFPAIAPFLADIDTSHGRGRVLYREDTSPAVLGLAARYVRAGFPRSARFTPTHAFLATWEQVGAYEEVKRGALPSGELNTFQAVLASDGSDSYALFLYPANGLQFLGTRPKESYNVQLQLPARVGFCRGEADDLKSEGPYFSLTSTEQSVKNLYQLSNLGIPGVWAFHIGSTSPLDNVRPAAVGDLSAAHSSVPLGRSFSHATALESDYNEDNLDYYDVNEEEAEYLPGEPEEALNGHSSIDVSFQSKVDTKPLEGRISPPDSDLSSPLHPTPTYWPFYPETESSTLDPHTKEGTSLGEVGGPDLKGQVEPWDERETRSPAPPEVDRDSLAPSWETPPPYPENGSIQPYPDGGPVPSEMDVPPAHPEEEIVLRSYPASGHTTPLSRGTYEVGLEDNIGSNTEVFTYNAANKETCEHNHRQCSRHAFCTDYATGFCCHCQSKFYGNGKHCLPEGAPHRVNGKVSGHLHVGHTPVHFTDVDLHAYIVGNDGRAYTAISHIPQPAAQALLPLTPIGGLFGWLFALEKPGSENGFSLAGAAFTHDMEVTFYPGEETVRITQTAEGLDPENYLSIKTNIQGQVPYVSANFTAHISPYKELYHYSDSTVTSTSSRDYSLTFGAINQTWSYRIHQNITYQVCRHAPRHPSFPTTQQLNVDRVFALYNDEERVLRFAVTNQIGPVKEDSDPTPGNPCYDGSHMCDTTARCHPGTGVDYTCECASGYQGDGRNCVDENECATGFHRCGPNSVCINLPGSYRCECRSGYEFADDRHTCILITPPANPCEDGSHTCAPAGQARCVHHGGSTFSCACLPGYAGDGHQCTDVDECSENRCHPAATCYNTPGSFSCRCQPGYYGDGFQCIPDSTSSLTPCEQQQRHAQAQYAYPGARFHIPQCDEQGNFLPLQCHGSTGFCWCVDPDGHEVPGTQTPPGSTPPHCGPSPEPTQRPPTICERWRENLLEHYGGTPRDDQYVPQCDDLGHFIPLQCHGKSDFCWCVDKDGREVQGTRSQPGTTPACIPTVAPPMVRPTPRPDVTPPSVGTFLLYTQGQQIGYLPLNGTRLQKDAAKTLLSLHGSIIVGIDYDCRERMVYWTDVAGRTISRAGLELGAEPETIVNSGLISPEGLAIDHIRRTMYWTDSVLDKIESALLDGSERKVLFYTDLVNPRAIAVDPIRGNLYWTDWNREAPKIETSSLDGENRRILINTDIGLPNGLTFDPFSKLLCWADAGTKKLECTLPDGTGRRVIQNNLKYPFSIVSYADHFYHTDWRRDGVVSVNKHSGQFTDEYLPEQRSHLYGITAVYPYCPTGRK

Target ORF information:

RefSeq Version	XM_005267405.5
Organism	Homo sapiens(human)
Definition	Homo sapiens nidogen 2 (NID2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005267405.5

ORF Insert Sequence:

ATGGAGGGGG ACCGGGTGGC CGGGCGGCCG GTGCTGTCGT CGTTACCAGT GCTACTGCTG 
CTGCCGTTGC TAATGTTGCG GGCCGCGGCG CTGCACCCAG ACGAGCTCTT CCCACACGGG 
GAGTCGTGGG GGGACCAGCT CCTGCAGGAA GGCGACGACG AAAGCTCAGC CGTGGTGAAG 
CTGGCGAATC CCCTGCACTT CTACGAAGCC CGATTCAGCA ACCTCTACGT GGGCACCAAC 
GGCATCATCT CCACTCAGGA CTTCCCCAGG GAAACGCAGT ATGTGGACTA TGATTTCCCC 
ACCGACTTCC CGGCCATCGC CCCTTTTCTG GCGGACATCG ACACGAGCCA CGGCAGAGGC 
CGAGTCCTGT ACCGAGAGGA CACCTCCCCC GCAGTGCTGG GCCTGGCCGC CCGCTATGTG 
CGCGCTGGCT TCCCGCGCTC TGCGCGCTTT ACCCCCACCC ACGCCTTCCT GGCCACCTGG 
GAGCAGGTAG GCGCTTACGA GGAGGTCAAG CGCGGGGCGC TGCCCTCGGG AGAGCTGAAC 
ACTTTCCAGG CAGTTTTGGC ATCTGATGGG TCTGATAGCT ACGCCCTCTT TCTTTATCCT 
GCCAACGGCC TGCAGTTCCT TGGAACCCGC CCCAAAGAGT CTTACAATGT CCAGCTTCAG 
CTTCCAGCTC GGGTGGGCTT CTGCCGAGGG GAGGCTGATG ATCTGAAGTC AGAAGGACCA 
TATTTCAGCT TGACTAGCAC TGAACAGTCT GTGAAAAATC TCTATCAACT AAGCAACCTG 
GGGATCCCTG GAGTGTGGGC TTTCCATATC GGCAGCACTT CCCCGTTGGA CAATGTCAGG 
CCAGCTGCAG TTGGAGACCT TTCCGCTGCC CACTCTTCTG TTCCCCTGGG ACGTTCCTTC 
AGCCATGCTA CAGCCCTGGA AAGTGACTAT AATGAGGACA ATTTGGATTA CTATGATGTG 
AATGAGGAGG AAGCTGAATA CCTTCCGGGT GAACCAGAGG AGGCATTGAA TGGCCACAGC 
AGCATTGATG TTTCCTTCCA ATCCAAAGTG GATACAAAGC CTTTAGAGGG TAGGATCTCC 
CCTCCAGATT CTGATCTGTC CTCCCCCTTG CATCCAACAC CTACTTATTG GCCATTCTAT 
CCTGAAACAG AATCTTCCAC CTTGGATCCT CACACCAAAG AAGGAACATC TCTGGGAGAG 
GTAGGGGGCC CAGATTTAAA AGGCCAAGTT GAGCCCTGGG ATGAGAGAGA GACCAGAAGC 
CCAGCTCCAC CAGAGGTAGA CAGAGATTCA CTGGCTCCTT CCTGGGAAAC CCCACCACCG 
TACCCCGAAA ACGGAAGCAT CCAGCCCTAC CCAGATGGAG GGCCAGTGCC TTCGGAAATG 
GATGTTCCCC CAGCTCATCC TGAAGAAGAA ATTGTTCTTC GAAGTTACCC TGCTTCAGGT 
CACACTACAC CCTTAAGTCG AGGGACGTAT GAGGTGGGAC TGGAAGACAA CATAGGTTCC 
AACACCGAGG TCTTCACGTA TAATGCTGCC AACAAGGAAA CCTGTGAACA CAACCACAGA 
CAATGCTCCC GGCATGCCTT CTGCACGGAC TATGCCACTG GCTTCTGCTG CCACTGCCAA 
TCCAAGTTTT ATGGAAATGG GAAGCACTGT CTGCCTGAAG GGGCACCTCA CCGAGTGAAT 
GGGAAAGTGA GTGGCCACCT CCACGTGGGC CATACACCCG TGCACTTCAC TGATGTGGAC 
CTGCATGCGT ATATCGTGGG CAATGATGGC AGAGCCTACA CGGCCATCAG CCACATCCCA 
CAGCCAGCAG CCCAGGCCCT CCTCCCCCTC ACACCAATTG GAGGCCTGTT TGGCTGGCTC 
TTTGCTTTAG AAAAACCTGG CTCTGAGAAC GGCTTCAGCC TCGCAGGTGC TGCCTTTACC 
CATGACATGG AAGTTACATT CTACCCGGGA GAGGAGACGG TTCGTATCAC TCAAACTGCT 
GAGGGACTTG ACCCAGAGAA CTACCTGAGC ATTAAGACCA ACATTCAAGG CCAGGTGCCT 
TACGTCTCAG CAAATTTCAC AGCCCACATC TCTCCCTACA AGGAGCTGTA CCACTACTCC 
GACTCCACTG TGACCTCTAC AAGTTCCAGA GACTACTCTC TGACTTTTGG TGCAATCAAC 
CAAACATGGT CCTACCGCAT CCACCAGAAC ATCACTTACC AGGTGTGCAG GCACGCCCCC 
AGACACCCGT CCTTCCCCAC CACCCAGCAG CTGAACGTGG ACCGGGTCTT TGCCTTGTAT 
AATGACGAAG AAAGAGTGCT TAGATTTGCT GTGACCAATC AAATTGGCCC GGTCAAAGAG 
GATTCAGACC CCACTCCGGG GAATCCTTGC TATGATGGGA GCCACATGTG TGACACAACA 
GCACGGTGCC ATCCAGGGAC AGGTGTAGAT TACACCTGTG AGTGCGCATC TGGGTACCAG 
GGAGATGGAC GGAACTGTGT GGATGAAAAT GAATGTGCAA CTGGCTTTCA TCGCTGTGGC 
CCCAACTCTG TATGTATCAA CTTGCCTGGA AGCTACAGGT GTGAGTGCCG GAGTGGTTAT 
GAGTTTGCAG ATGACCGGCA TACTTGCATC TTGATCACCC CACCTGCCAA CCCCTGTGAG 
GATGGCAGTC ATACCTGTGC TCCTGCTGGG CAGGCCCGGT GTGTTCACCA TGGAGGCAGC 
ACGTTCAGCT GTGCCTGCCT GCCTGGTTAT GCCGGCGATG GGCACCAGTG CACTGATGTA 
GATGAATGCT CAGAAAACAG ATGTCACCCT GCAGCTACCT GCTACAATAC TCCTGGTTCC 
TTCTCCTGCC GTTGTCAACC CGGATATTAT GGGGATGGAT TTCAGTGCAT ACCTGACTCC 
ACCTCAAGCC TGACACCCTG TGAACAACAG CAGCGCCATG CCCAGGCCCA GTATGCCTAC 
CCTGGGGCCC GGTTCCACAT CCCCCAATGC GACGAGCAGG GCAACTTCCT GCCCCTACAG 
TGTCATGGCA GCACTGGTTT CTGCTGGTGC GTGGACCCTG ATGGTCATGA AGTTCCTGGT 
ACCCAGACTC CACCTGGCTC CACCCCGCCT CACTGTGGAC CATCACCAGA GCCCACCCAG 
AGGCCCCCGA CCATCTGTGA GCGCTGGAGG GAAAACCTGC TGGAGCACTA CGGTGGCACC 
CCCCGGGATG ACCAGTACGT GCCCCAGTGC GATGACCTGG GCCACTTCAT CCCCCTGCAG 
TGCCACGGAA AGAGCGACTT CTGCTGGTGT GTGGACAAAG ATGGCAGAGA GGTGCAGGGC 
ACCCGCTCCC AGCCAGGCAC CACCCCTGCG TGTATACCCA CCGTCGCTCC ACCCATGGTC 
CGGCCCACGC CCCGGCCAGA TGTGACCCCT CCATCTGTGG GCACCTTCCT GCTCTATACT 
CAGGGCCAGC AGATTGGCTA CTTACCCCTC AATGGCACCA GGCTTCAGAA GGATGCAGCT 
AAGACCCTGC TGTCTCTGCA TGGCTCCATA ATCGTGGGAA TTGATTACGA CTGCCGGGAG 
AGGATGGTGT ACTGGACAGA TGTTGCTGGA CGGACAATCA GCCGTGCTGG TCTGGAACTG 
GGAGCAGAGC CTGAGACGAT CGTGAATTCA GGTCTGATAA GCCCTGAAGG ACTTGCCATA 
GACCACATCC GCAGAACAAT GTACTGGACG GACAGTGTCC TGGATAAGAT AGAGAGCGCC 
CTGCTGGATG GCTCTGAGCG CAAGGTCCTC TTCTACACAG ATCTGGTGAA TCCCCGTGCC 
ATCGCTGTGG ATCCAATCCG AGGCAACTTG TACTGGACAG ACTGGAATAG AGAAGCTCCT 
AAAATTGAAA CGTCATCTTT AGATGGAGAA AACAGAAGAA TTCTGATCAA TACAGACATT 
GGATTGCCCA ATGGCTTAAC CTTTGACCCT TTCTCTAAAC TGCTCTGCTG GGCAGATGCA 
GGAACCAAAA AACTGGAGTG TACACTACCT GATGGAACTG GACGGCGTGT CATTCAAAAC 
AACCTCAAGT ACCCCTTCAG CATCGTAAGC TATGCAGATC ACTTCTACCA CACAGACTGG 
AGGAGGGATG GTGTTGTATC AGTAAATAAA CATAGTGGCC AGTTTACTGA TGAGTATCTC 
CCAGAACAAC GATCTCACCT CTACGGGATA ACTGCAGTCT ACCCCTACTG CCCAACAGGA 
AGAAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NID2 ( XM_005267406.4 ) cDNA ORF clone, Homo sapiens(human) -> XP_005267463.1 Homo sapiens nidogen 2 (NID2), transcript variant X2, mRNA.

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CloneID	OHu47582
Clone ID Related Accession (Same CDS sequence)	XM_005267406.4 , XM_005267406.5
Accession Version	XM_005267406.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4065bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	nidogen-2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000014.9) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005267406.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGGGG ACCGGGTGGC CGGGCGGCCG GTGCTGTCGT CGTTACCAGT GCTACTGCTG 
CTGCCGTTGC TAATGTTGCG GGCCGCGGCG CTGCACCCAG ACGAGCTCTT CCCACACGGG 
GAGTCGTGGG GGGACCAGCT CCTGCAGGAA GGCGACGACG AAAGCTCAGC CGTGGTGAAG 
CTGGCGAATC CCCTGCACTT CTACGAAGCC CGATTCAGCA ACCTCTACGT GGGCACCAAC 
GGCATCATCT CCACTCAGGA CTTCCCCAGG GAAACGCAGT ATGTGGACTA TGATTTCCCC 
ACCGACTTCC CGGCCATCGC CCCTTTTCTG GCGGACATCG ACACGAGCCA CGGCAGAGGC 
CGAGTCCTGT ACCGAGAGGA CACCTCCCCC GCAGTGCTGG GCCTGGCCGC CCGCTATGTG 
CGCGCTGGCT TCCCGCGCTC TGCGCGCTTT ACCCCCACCC ACGCCTTCCT GGCCACCTGG 
GAGCAGGTAG GCGCTTACGA GGAGGTCAAG CGCGGGGCGC TGCCCTCGGG AGAGCTGAAC 
ACTTTCCAGG CAGTTTTGGC ATCTGATGGG TCTGATAGCT ACGCCCTCTT TCTTTATCCT 
GCCAACGGCC TGCAGTTCCT TGGAACCCGC CCCAAAGAGT CTTACAATGT CCAGCTTCAG 
CTTCCAGCTC GGGTGGGCTT CTGCCGAGGG GAGGCTGATG ATCTGAAGTC AGAAGGACCA 
TATTTCAGCT TGACTAGCAC TGAACAGTCT GTGAAAAATC TCTATCAACT AAGCAACCTG 
GGGATCCCTG GAGTGTGGGC TTTCCATATC GGCAGCACTT CCCCGTTGGA CAATGTCAGG 
CCAGCTGCAG TTGGAGACCT TTCCGCTGCC CACTCTTCTG TTCCCCTGGG ACGTTCCTTC 
AGCCATGCTA CAGCCCTGGA AAGTGACTAT AATGAGGACA ATTTGGATTA CTATGATGTG 
AATGAGGAGG AAGCTGAATA CCTTCCGGGT GAACCAGAGG AGGCATTGAA TGGCCACAGC 
AGCATTGATG TTTCCTTCCA ATCCAAAGTG GATACAAAGC CTTTAGAGGG TAGGATCTCC 
CCTCCAGATT CTGATCTGTC CTCCCCCTTG CATCCAACAC CTACTTATTG GCCATTCTAT 
CCTGAAACAG AATCTTCCAC CTTGGATCCT CACACCAAAG AAGGAACATC TCTGGGAGAG 
GTAGGGGGCC CAGATTTAAA AGGCCAAGTT GAGCCCTGGG ATGAGAGAGA GACCAGAAGC 
CCAGCTCCAC CAGAGGTAGA CAGAGATTCA CTGGCTCCTT CCTGGGAAAC CCCACCACCG 
TACCCCGAAA ACGGAAGCAT CCAGCCCTAC CCAGATGGAG GGCCAGTGCC TTCGGAAATG 
GATGTTCCCC CAGCTCATCC TGAAGAAGAA ATTGTTCTTC GAAGTTACCC TGCTTCAGGT 
CACACTACAC CCTTAAGTCG AGGGACGTAT GAGGTGGGAC TGGAAGACAA CATAGGTTCC 
AACACCGAGG TCTTCACGTA TAATGCTGCC AACAAGGAAA CCTGTGAACA CAACCACAGA 
CAATGCTCCC GGCATGCCTT CTGCACGGAC TATGCCACTG GCTTCTGCTG CCACTGCCAA 
TCCAAGTTTT ATGGAAATGG GAAGCACTGT CTGCCTGAAG GGGCACCTCA CCGAGTGAAT 
GGGAAAGTGA GTGGCCACCT CCACGTGGGC CATACACCCG TGCACTTCAC TGATGTGGAC 
CTGCATGCGT ATATCGTGGG CAATGATGGC AGAGCCTACA CGGCCATCAG CCACATCCCA 
CAGCCAGCAG CCCAGGCCCT CCTCCCCCTC ACACCAATTG GAGGCCTGTT TGGCTGGCTC 
TTTGCTTTAG AAAAACCTGG CTCTGAGAAC GGCTTCAGCC TCGCAGGTGC TGCCTTTACC 
CATGACATGG AAGTTACATT CTACCCGGGA GAGGAGACGG TTCGTATCAC TCAAACTGCT 
GAGGGACTTG ACCCAGAGAA CTACCTGAGC ATTAAGACCA ACATTCAAGG CCAGGTGCCT 
TACGTCTCAG CAAATTTCAC AGCCCACATC TCTCCCTACA AGGAGCTGTA CCACTACTCC 
GACTCCACTG TGACCTCTAC AAGTTCCAGA GACTACTCTC TGACTTTTGG TGCAATCAAC 
CAAACATGGT CCTACCGCAT CCACCAGAAC ATCACTTACC AGGTGTGCAG GCACGCCCCC 
AGACACCCGT CCTTCCCCAC CACCCAGCAG CTGAACGTGG ACCGGGTCTT TGCCTTGTAT 
AATGACGAAG AAAGAGTGCT TAGATTTGCT GTGACCAATC AAATTGGCCC GGTCAAAGAG 
GATTCAGACC CCACTCCGGG GAATCCTTGC TATGATGGGA GCCACATGTG TGACACAACA 
GCACGGTGCC ATCCAGGGAC AGGTGTAGAT TACACCTGTG AGTGCGCATC TGGGTACCAG 
GGAGATGGAC GGAACTGTGT GGATGAAAAT GAATGTGCAA CTGGCTTTCA TCGCTGTGGC 
CCCAACTCTG TATGTATCAA CTTGCCTGGA AGCTACAGGT GTGAGTGCCG GAGTGGTTAT 
GAGTTTGCAG ATGACCGGCA TACTTGCATC TATGTAGATG AATGCTCAGA AAACAGATGT 
CACCCTGCAG CTACCTGCTA CAATACTCCT GGTTCCTTCT CCTGCCGTTG TCAACCCGGA 
TATTATGGGG ATGGATTTCA GTGCATACCT GACTCCACCT CAAGCCTGAC ACCCTGTGAA 
CAACAGCAGC GCCATGCCCA GGCCCAGTAT GCCTACCCTG GGGCCCGGTT CCACATCCCC 
CAATGCGACG AGCAGGGCAA CTTCCTGCCC CTACAGTGTC ATGGCAGCAC TGGTTTCTGC 
TGGTGCGTGG ACCCTGATGG TCATGAAGTT CCTGGTACCC AGACTCCACC TGGCTCCACC 
CCGCCTCACT GTGGACCATC ACCAGAGCCC ACCCAGAGGC CCCCGACCAT CTGTGAGCGC 
TGGAGGGAAA ACCTGCTGGA GCACTACGGT GGCACCCCCC GGGATGACCA GTACGTGCCC 
CAGTGCGATG ACCTGGGCCA CTTCATCCCC CTGCAGTGCC ACGGAAAGAG CGACTTCTGC 
TGGTGTGTGG ACAAAGATGG CAGAGAGGTG CAGGGCACCC GCTCCCAGCC AGGCACCACC 
CCTGCGTGTA TACCCACCGT CGCTCCACCC ATGGTCCGGC CCACGCCCCG GCCAGATGTG 
ACCCCTCCAT CTGTGGGCAC CTTCCTGCTC TATACTCAGG GCCAGCAGAT TGGCTACTTA 
CCCCTCAATG GCACCAGGCT TCAGAAGGAT GCAGCTAAGA CCCTGCTGTC TCTGCATGGC 
TCCATAATCG TGGGAATTGA TTACGACTGC CGGGAGAGGA TGGTGTACTG GACAGATGTT 
GCTGGACGGA CAATCAGCCG TGCTGGTCTG GAACTGGGAG CAGAGCCTGA GACGATCGTG 
AATTCAGGTC TGATAAGCCC TGAAGGACTT GCCATAGACC ACATCCGCAG AACAATGTAC 
TGGACGGACA GTGTCCTGGA TAAGATAGAG AGCGCCCTGC TGGATGGCTC TGAGCGCAAG 
GTCCTCTTCT ACACAGATCT GGTGAATCCC CGTGCCATCG CTGTGGATCC AATCCGAGGC 
AACTTGTACT GGACAGACTG GAATAGAGAA GCTCCTAAAA TTGAAACGTC ATCTTTAGAT 
GGAGAAAACA GAAGAATTCT GATCAATACA GACATTGGAT TGCCCAATGG CTTAACCTTT 
GACCCTTTCT CTAAACTGCT CTGCTGGGCA GATGCAGGAA CCAAAAAACT GGAGTGTACA 
CTACCTGATG GAACTGGACG GCGTGTCATT CAAAACAACC TCAAGTACCC CTTCAGCATC 
GTAAGCTATG CAGATCACTT CTACCACACA GACTGGAGGA GGGATGGTGT TGTATCAGTA 
AATAAACATA GTGGCCAGTT TACTGATGAG TATCTCCCAG AACAACGATC TCACCTCTAC 
GGGATAACTG CAGTCTACCC CTACTGCCCA ACAGGAAGAA AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005267463.1
CDS	238..4302
Translation	MEGDRVAGRPVLSSLPVLLLLPLLMLRAAALHPDELFPHGESWGDQLLQEGDDESSAVVKLANPLHFYEARFSNLYVGTNGIISTQDFPRETQYVDYDFPTDFPAIAPFLADIDTSHGRGRVLYREDTSPAVLGLAARYVRAGFPRSARFTPTHAFLATWEQVGAYEEVKRGALPSGELNTFQAVLASDGSDSYALFLYPANGLQFLGTRPKESYNVQLQLPARVGFCRGEADDLKSEGPYFSLTSTEQSVKNLYQLSNLGIPGVWAFHIGSTSPLDNVRPAAVGDLSAAHSSVPLGRSFSHATALESDYNEDNLDYYDVNEEEAEYLPGEPEEALNGHSSIDVSFQSKVDTKPLEGRISPPDSDLSSPLHPTPTYWPFYPETESSTLDPHTKEGTSLGEVGGPDLKGQVEPWDERETRSPAPPEVDRDSLAPSWETPPPYPENGSIQPYPDGGPVPSEMDVPPAHPEEEIVLRSYPASGHTTPLSRGTYEVGLEDNIGSNTEVFTYNAANKETCEHNHRQCSRHAFCTDYATGFCCHCQSKFYGNGKHCLPEGAPHRVNGKVSGHLHVGHTPVHFTDVDLHAYIVGNDGRAYTAISHIPQPAAQALLPLTPIGGLFGWLFALEKPGSENGFSLAGAAFTHDMEVTFYPGEETVRITQTAEGLDPENYLSIKTNIQGQVPYVSANFTAHISPYKELYHYSDSTVTSTSSRDYSLTFGAINQTWSYRIHQNITYQVCRHAPRHPSFPTTQQLNVDRVFALYNDEERVLRFAVTNQIGPVKEDSDPTPGNPCYDGSHMCDTTARCHPGTGVDYTCECASGYQGDGRNCVDENECATGFHRCGPNSVCINLPGSYRCECRSGYEFADDRHTCIYVDECSENRCHPAATCYNTPGSFSCRCQPGYYGDGFQCIPDSTSSLTPCEQQQRHAQAQYAYPGARFHIPQCDEQGNFLPLQCHGSTGFCWCVDPDGHEVPGTQTPPGSTPPHCGPSPEPTQRPPTICERWRENLLEHYGGTPRDDQYVPQCDDLGHFIPLQCHGKSDFCWCVDKDGREVQGTRSQPGTTPACIPTVAPPMVRPTPRPDVTPPSVGTFLLYTQGQQIGYLPLNGTRLQKDAAKTLLSLHGSIIVGIDYDCRERMVYWTDVAGRTISRAGLELGAEPETIVNSGLISPEGLAIDHIRRTMYWTDSVLDKIESALLDGSERKVLFYTDLVNPRAIAVDPIRGNLYWTDWNREAPKIETSSLDGENRRILINTDIGLPNGLTFDPFSKLLCWADAGTKKLECTLPDGTGRRVIQNNLKYPFSIVSYADHFYHTDWRRDGVVSVNKHSGQFTDEYLPEQRSHLYGITAVYPYCPTGRK

Target ORF information:

RefSeq Version	XM_005267406.4
Organism	Homo sapiens(human)
Definition	Homo sapiens nidogen 2 (NID2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005267406.4

ORF Insert Sequence:

ATGGAGGGGG ACCGGGTGGC CGGGCGGCCG GTGCTGTCGT CGTTACCAGT GCTACTGCTG 
CTGCCGTTGC TAATGTTGCG GGCCGCGGCG CTGCACCCAG ACGAGCTCTT CCCACACGGG 
GAGTCGTGGG GGGACCAGCT CCTGCAGGAA GGCGACGACG AAAGCTCAGC CGTGGTGAAG 
CTGGCGAATC CCCTGCACTT CTACGAAGCC CGATTCAGCA ACCTCTACGT GGGCACCAAC 
GGCATCATCT CCACTCAGGA CTTCCCCAGG GAAACGCAGT ATGTGGACTA TGATTTCCCC 
ACCGACTTCC CGGCCATCGC CCCTTTTCTG GCGGACATCG ACACGAGCCA CGGCAGAGGC 
CGAGTCCTGT ACCGAGAGGA CACCTCCCCC GCAGTGCTGG GCCTGGCCGC CCGCTATGTG 
CGCGCTGGCT TCCCGCGCTC TGCGCGCTTT ACCCCCACCC ACGCCTTCCT GGCCACCTGG 
GAGCAGGTAG GCGCTTACGA GGAGGTCAAG CGCGGGGCGC TGCCCTCGGG AGAGCTGAAC 
ACTTTCCAGG CAGTTTTGGC ATCTGATGGG TCTGATAGCT ACGCCCTCTT TCTTTATCCT 
GCCAACGGCC TGCAGTTCCT TGGAACCCGC CCCAAAGAGT CTTACAATGT CCAGCTTCAG 
CTTCCAGCTC GGGTGGGCTT CTGCCGAGGG GAGGCTGATG ATCTGAAGTC AGAAGGACCA 
TATTTCAGCT TGACTAGCAC TGAACAGTCT GTGAAAAATC TCTATCAACT AAGCAACCTG 
GGGATCCCTG GAGTGTGGGC TTTCCATATC GGCAGCACTT CCCCGTTGGA CAATGTCAGG 
CCAGCTGCAG TTGGAGACCT TTCCGCTGCC CACTCTTCTG TTCCCCTGGG ACGTTCCTTC 
AGCCATGCTA CAGCCCTGGA AAGTGACTAT AATGAGGACA ATTTGGATTA CTATGATGTG 
AATGAGGAGG AAGCTGAATA CCTTCCGGGT GAACCAGAGG AGGCATTGAA TGGCCACAGC 
AGCATTGATG TTTCCTTCCA ATCCAAAGTG GATACAAAGC CTTTAGAGGG TAGGATCTCC 
CCTCCAGATT CTGATCTGTC CTCCCCCTTG CATCCAACAC CTACTTATTG GCCATTCTAT 
CCTGAAACAG AATCTTCCAC CTTGGATCCT CACACCAAAG AAGGAACATC TCTGGGAGAG 
GTAGGGGGCC CAGATTTAAA AGGCCAAGTT GAGCCCTGGG ATGAGAGAGA GACCAGAAGC 
CCAGCTCCAC CAGAGGTAGA CAGAGATTCA CTGGCTCCTT CCTGGGAAAC CCCACCACCG 
TACCCCGAAA ACGGAAGCAT CCAGCCCTAC CCAGATGGAG GGCCAGTGCC TTCGGAAATG 
GATGTTCCCC CAGCTCATCC TGAAGAAGAA ATTGTTCTTC GAAGTTACCC TGCTTCAGGT 
CACACTACAC CCTTAAGTCG AGGGACGTAT GAGGTGGGAC TGGAAGACAA CATAGGTTCC 
AACACCGAGG TCTTCACGTA TAATGCTGCC AACAAGGAAA CCTGTGAACA CAACCACAGA 
CAATGCTCCC GGCATGCCTT CTGCACGGAC TATGCCACTG GCTTCTGCTG CCACTGCCAA 
TCCAAGTTTT ATGGAAATGG GAAGCACTGT CTGCCTGAAG GGGCACCTCA CCGAGTGAAT 
GGGAAAGTGA GTGGCCACCT CCACGTGGGC CATACACCCG TGCACTTCAC TGATGTGGAC 
CTGCATGCGT ATATCGTGGG CAATGATGGC AGAGCCTACA CGGCCATCAG CCACATCCCA 
CAGCCAGCAG CCCAGGCCCT CCTCCCCCTC ACACCAATTG GAGGCCTGTT TGGCTGGCTC 
TTTGCTTTAG AAAAACCTGG CTCTGAGAAC GGCTTCAGCC TCGCAGGTGC TGCCTTTACC 
CATGACATGG AAGTTACATT CTACCCGGGA GAGGAGACGG TTCGTATCAC TCAAACTGCT 
GAGGGACTTG ACCCAGAGAA CTACCTGAGC ATTAAGACCA ACATTCAAGG CCAGGTGCCT 
TACGTCTCAG CAAATTTCAC AGCCCACATC TCTCCCTACA AGGAGCTGTA CCACTACTCC 
GACTCCACTG TGACCTCTAC AAGTTCCAGA GACTACTCTC TGACTTTTGG TGCAATCAAC 
CAAACATGGT CCTACCGCAT CCACCAGAAC ATCACTTACC AGGTGTGCAG GCACGCCCCC 
AGACACCCGT CCTTCCCCAC CACCCAGCAG CTGAACGTGG ACCGGGTCTT TGCCTTGTAT 
AATGACGAAG AAAGAGTGCT TAGATTTGCT GTGACCAATC AAATTGGCCC GGTCAAAGAG 
GATTCAGACC CCACTCCGGG GAATCCTTGC TATGATGGGA GCCACATGTG TGACACAACA 
GCACGGTGCC ATCCAGGGAC AGGTGTAGAT TACACCTGTG AGTGCGCATC TGGGTACCAG 
GGAGATGGAC GGAACTGTGT GGATGAAAAT GAATGTGCAA CTGGCTTTCA TCGCTGTGGC 
CCCAACTCTG TATGTATCAA CTTGCCTGGA AGCTACAGGT GTGAGTGCCG GAGTGGTTAT 
GAGTTTGCAG ATGACCGGCA TACTTGCATC TATGTAGATG AATGCTCAGA AAACAGATGT 
CACCCTGCAG CTACCTGCTA CAATACTCCT GGTTCCTTCT CCTGCCGTTG TCAACCCGGA 
TATTATGGGG ATGGATTTCA GTGCATACCT GACTCCACCT CAAGCCTGAC ACCCTGTGAA 
CAACAGCAGC GCCATGCCCA GGCCCAGTAT GCCTACCCTG GGGCCCGGTT CCACATCCCC 
CAATGCGACG AGCAGGGCAA CTTCCTGCCC CTACAGTGTC ATGGCAGCAC TGGTTTCTGC 
TGGTGCGTGG ACCCTGATGG TCATGAAGTT CCTGGTACCC AGACTCCACC TGGCTCCACC 
CCGCCTCACT GTGGACCATC ACCAGAGCCC ACCCAGAGGC CCCCGACCAT CTGTGAGCGC 
TGGAGGGAAA ACCTGCTGGA GCACTACGGT GGCACCCCCC GGGATGACCA GTACGTGCCC 
CAGTGCGATG ACCTGGGCCA CTTCATCCCC CTGCAGTGCC ACGGAAAGAG CGACTTCTGC 
TGGTGTGTGG ACAAAGATGG CAGAGAGGTG CAGGGCACCC GCTCCCAGCC AGGCACCACC 
CCTGCGTGTA TACCCACCGT CGCTCCACCC ATGGTCCGGC CCACGCCCCG GCCAGATGTG 
ACCCCTCCAT CTGTGGGCAC CTTCCTGCTC TATACTCAGG GCCAGCAGAT TGGCTACTTA 
CCCCTCAATG GCACCAGGCT TCAGAAGGAT GCAGCTAAGA CCCTGCTGTC TCTGCATGGC 
TCCATAATCG TGGGAATTGA TTACGACTGC CGGGAGAGGA TGGTGTACTG GACAGATGTT 
GCTGGACGGA CAATCAGCCG TGCTGGTCTG GAACTGGGAG CAGAGCCTGA GACGATCGTG 
AATTCAGGTC TGATAAGCCC TGAAGGACTT GCCATAGACC ACATCCGCAG AACAATGTAC 
TGGACGGACA GTGTCCTGGA TAAGATAGAG AGCGCCCTGC TGGATGGCTC TGAGCGCAAG 
GTCCTCTTCT ACACAGATCT GGTGAATCCC CGTGCCATCG CTGTGGATCC AATCCGAGGC 
AACTTGTACT GGACAGACTG GAATAGAGAA GCTCCTAAAA TTGAAACGTC ATCTTTAGAT 
GGAGAAAACA GAAGAATTCT GATCAATACA GACATTGGAT TGCCCAATGG CTTAACCTTT 
GACCCTTTCT CTAAACTGCT CTGCTGGGCA GATGCAGGAA CCAAAAAACT GGAGTGTACA 
CTACCTGATG GAACTGGACG GCGTGTCATT CAAAACAACC TCAAGTACCC CTTCAGCATC 
GTAAGCTATG CAGATCACTT CTACCACACA GACTGGAGGA GGGATGGTGT TGTATCAGTA 
AATAAACATA GTGGCCAGTT TACTGATGAG TATCTCCCAG AACAACGATC TCACCTCTAC 
GGGATAACTG CAGTCTACCC CTACTGCCCA ACAGGAAGAA AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NID2 ( XM_005267406.5 ) cDNA ORF clone, Homo sapiens(human) -> XP_005267463.1 Homo sapiens nidogen 2 (NID2), transcript variant X2, mRNA.

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CloneID	OHu47582
Clone ID Related Accession (Same CDS sequence)	XM_005267406.4 , XM_005267406.5
Accession Version	XM_005267406.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4065bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	nidogen-2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000014.9) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_005267406.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGGGG ACCGGGTGGC CGGGCGGCCG GTGCTGTCGT CGTTACCAGT GCTACTGCTG 
CTGCCGTTGC TAATGTTGCG GGCCGCGGCG CTGCACCCAG ACGAGCTCTT CCCACACGGG 
GAGTCGTGGG GGGACCAGCT CCTGCAGGAA GGCGACGACG AAAGCTCAGC CGTGGTGAAG 
CTGGCGAATC CCCTGCACTT CTACGAAGCC CGATTCAGCA ACCTCTACGT GGGCACCAAC 
GGCATCATCT CCACTCAGGA CTTCCCCAGG GAAACGCAGT ATGTGGACTA TGATTTCCCC 
ACCGACTTCC CGGCCATCGC CCCTTTTCTG GCGGACATCG ACACGAGCCA CGGCAGAGGC 
CGAGTCCTGT ACCGAGAGGA CACCTCCCCC GCAGTGCTGG GCCTGGCCGC CCGCTATGTG 
CGCGCTGGCT TCCCGCGCTC TGCGCGCTTT ACCCCCACCC ACGCCTTCCT GGCCACCTGG 
GAGCAGGTAG GCGCTTACGA GGAGGTCAAG CGCGGGGCGC TGCCCTCGGG AGAGCTGAAC 
ACTTTCCAGG CAGTTTTGGC ATCTGATGGG TCTGATAGCT ACGCCCTCTT TCTTTATCCT 
GCCAACGGCC TGCAGTTCCT TGGAACCCGC CCCAAAGAGT CTTACAATGT CCAGCTTCAG 
CTTCCAGCTC GGGTGGGCTT CTGCCGAGGG GAGGCTGATG ATCTGAAGTC AGAAGGACCA 
TATTTCAGCT TGACTAGCAC TGAACAGTCT GTGAAAAATC TCTATCAACT AAGCAACCTG 
GGGATCCCTG GAGTGTGGGC TTTCCATATC GGCAGCACTT CCCCGTTGGA CAATGTCAGG 
CCAGCTGCAG TTGGAGACCT TTCCGCTGCC CACTCTTCTG TTCCCCTGGG ACGTTCCTTC 
AGCCATGCTA CAGCCCTGGA AAGTGACTAT AATGAGGACA ATTTGGATTA CTATGATGTG 
AATGAGGAGG AAGCTGAATA CCTTCCGGGT GAACCAGAGG AGGCATTGAA TGGCCACAGC 
AGCATTGATG TTTCCTTCCA ATCCAAAGTG GATACAAAGC CTTTAGAGGG TAGGATCTCC 
CCTCCAGATT CTGATCTGTC CTCCCCCTTG CATCCAACAC CTACTTATTG GCCATTCTAT 
CCTGAAACAG AATCTTCCAC CTTGGATCCT CACACCAAAG AAGGAACATC TCTGGGAGAG 
GTAGGGGGCC CAGATTTAAA AGGCCAAGTT GAGCCCTGGG ATGAGAGAGA GACCAGAAGC 
CCAGCTCCAC CAGAGGTAGA CAGAGATTCA CTGGCTCCTT CCTGGGAAAC CCCACCACCG 
TACCCCGAAA ACGGAAGCAT CCAGCCCTAC CCAGATGGAG GGCCAGTGCC TTCGGAAATG 
GATGTTCCCC CAGCTCATCC TGAAGAAGAA ATTGTTCTTC GAAGTTACCC TGCTTCAGGT 
CACACTACAC CCTTAAGTCG AGGGACGTAT GAGGTGGGAC TGGAAGACAA CATAGGTTCC 
AACACCGAGG TCTTCACGTA TAATGCTGCC AACAAGGAAA CCTGTGAACA CAACCACAGA 
CAATGCTCCC GGCATGCCTT CTGCACGGAC TATGCCACTG GCTTCTGCTG CCACTGCCAA 
TCCAAGTTTT ATGGAAATGG GAAGCACTGT CTGCCTGAAG GGGCACCTCA CCGAGTGAAT 
GGGAAAGTGA GTGGCCACCT CCACGTGGGC CATACACCCG TGCACTTCAC TGATGTGGAC 
CTGCATGCGT ATATCGTGGG CAATGATGGC AGAGCCTACA CGGCCATCAG CCACATCCCA 
CAGCCAGCAG CCCAGGCCCT CCTCCCCCTC ACACCAATTG GAGGCCTGTT TGGCTGGCTC 
TTTGCTTTAG AAAAACCTGG CTCTGAGAAC GGCTTCAGCC TCGCAGGTGC TGCCTTTACC 
CATGACATGG AAGTTACATT CTACCCGGGA GAGGAGACGG TTCGTATCAC TCAAACTGCT 
GAGGGACTTG ACCCAGAGAA CTACCTGAGC ATTAAGACCA ACATTCAAGG CCAGGTGCCT 
TACGTCTCAG CAAATTTCAC AGCCCACATC TCTCCCTACA AGGAGCTGTA CCACTACTCC 
GACTCCACTG TGACCTCTAC AAGTTCCAGA GACTACTCTC TGACTTTTGG TGCAATCAAC 
CAAACATGGT CCTACCGCAT CCACCAGAAC ATCACTTACC AGGTGTGCAG GCACGCCCCC 
AGACACCCGT CCTTCCCCAC CACCCAGCAG CTGAACGTGG ACCGGGTCTT TGCCTTGTAT 
AATGACGAAG AAAGAGTGCT TAGATTTGCT GTGACCAATC AAATTGGCCC GGTCAAAGAG 
GATTCAGACC CCACTCCGGG GAATCCTTGC TATGATGGGA GCCACATGTG TGACACAACA 
GCACGGTGCC ATCCAGGGAC AGGTGTAGAT TACACCTGTG AGTGCGCATC TGGGTACCAG 
GGAGATGGAC GGAACTGTGT GGATGAAAAT GAATGTGCAA CTGGCTTTCA TCGCTGTGGC 
CCCAACTCTG TATGTATCAA CTTGCCTGGA AGCTACAGGT GTGAGTGCCG GAGTGGTTAT 
GAGTTTGCAG ATGACCGGCA TACTTGCATC TATGTAGATG AATGCTCAGA AAACAGATGT 
CACCCTGCAG CTACCTGCTA CAATACTCCT GGTTCCTTCT CCTGCCGTTG TCAACCCGGA 
TATTATGGGG ATGGATTTCA GTGCATACCT GACTCCACCT CAAGCCTGAC ACCCTGTGAA 
CAACAGCAGC GCCATGCCCA GGCCCAGTAT GCCTACCCTG GGGCCCGGTT CCACATCCCC 
CAATGCGACG AGCAGGGCAA CTTCCTGCCC CTACAGTGTC ATGGCAGCAC TGGTTTCTGC 
TGGTGCGTGG ACCCTGATGG TCATGAAGTT CCTGGTACCC AGACTCCACC TGGCTCCACC 
CCGCCTCACT GTGGACCATC ACCAGAGCCC ACCCAGAGGC CCCCGACCAT CTGTGAGCGC 
TGGAGGGAAA ACCTGCTGGA GCACTACGGT GGCACCCCCC GGGATGACCA GTACGTGCCC 
CAGTGCGATG ACCTGGGCCA CTTCATCCCC CTGCAGTGCC ACGGAAAGAG CGACTTCTGC 
TGGTGTGTGG ACAAAGATGG CAGAGAGGTG CAGGGCACCC GCTCCCAGCC AGGCACCACC 
CCTGCGTGTA TACCCACCGT CGCTCCACCC ATGGTCCGGC CCACGCCCCG GCCAGATGTG 
ACCCCTCCAT CTGTGGGCAC CTTCCTGCTC TATACTCAGG GCCAGCAGAT TGGCTACTTA 
CCCCTCAATG GCACCAGGCT TCAGAAGGAT GCAGCTAAGA CCCTGCTGTC TCTGCATGGC 
TCCATAATCG TGGGAATTGA TTACGACTGC CGGGAGAGGA TGGTGTACTG GACAGATGTT 
GCTGGACGGA CAATCAGCCG TGCTGGTCTG GAACTGGGAG CAGAGCCTGA GACGATCGTG 
AATTCAGGTC TGATAAGCCC TGAAGGACTT GCCATAGACC ACATCCGCAG AACAATGTAC 
TGGACGGACA GTGTCCTGGA TAAGATAGAG AGCGCCCTGC TGGATGGCTC TGAGCGCAAG 
GTCCTCTTCT ACACAGATCT GGTGAATCCC CGTGCCATCG CTGTGGATCC AATCCGAGGC 
AACTTGTACT GGACAGACTG GAATAGAGAA GCTCCTAAAA TTGAAACGTC ATCTTTAGAT 
GGAGAAAACA GAAGAATTCT GATCAATACA GACATTGGAT TGCCCAATGG CTTAACCTTT 
GACCCTTTCT CTAAACTGCT CTGCTGGGCA GATGCAGGAA CCAAAAAACT GGAGTGTACA 
CTACCTGATG GAACTGGACG GCGTGTCATT CAAAACAACC TCAAGTACCC CTTCAGCATC 
GTAAGCTATG CAGATCACTT CTACCACACA GACTGGAGGA GGGATGGTGT TGTATCAGTA 
AATAAACATA GTGGCCAGTT TACTGATGAG TATCTCCCAG AACAACGATC TCACCTCTAC 
GGGATAACTG CAGTCTACCC CTACTGCCCA ACAGGAAGAA AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005267463.1
CDS	66..4130
Translation	MEGDRVAGRPVLSSLPVLLLLPLLMLRAAALHPDELFPHGESWGDQLLQEGDDESSAVVKLANPLHFYEARFSNLYVGTNGIISTQDFPRETQYVDYDFPTDFPAIAPFLADIDTSHGRGRVLYREDTSPAVLGLAARYVRAGFPRSARFTPTHAFLATWEQVGAYEEVKRGALPSGELNTFQAVLASDGSDSYALFLYPANGLQFLGTRPKESYNVQLQLPARVGFCRGEADDLKSEGPYFSLTSTEQSVKNLYQLSNLGIPGVWAFHIGSTSPLDNVRPAAVGDLSAAHSSVPLGRSFSHATALESDYNEDNLDYYDVNEEEAEYLPGEPEEALNGHSSIDVSFQSKVDTKPLEGRISPPDSDLSSPLHPTPTYWPFYPETESSTLDPHTKEGTSLGEVGGPDLKGQVEPWDERETRSPAPPEVDRDSLAPSWETPPPYPENGSIQPYPDGGPVPSEMDVPPAHPEEEIVLRSYPASGHTTPLSRGTYEVGLEDNIGSNTEVFTYNAANKETCEHNHRQCSRHAFCTDYATGFCCHCQSKFYGNGKHCLPEGAPHRVNGKVSGHLHVGHTPVHFTDVDLHAYIVGNDGRAYTAISHIPQPAAQALLPLTPIGGLFGWLFALEKPGSENGFSLAGAAFTHDMEVTFYPGEETVRITQTAEGLDPENYLSIKTNIQGQVPYVSANFTAHISPYKELYHYSDSTVTSTSSRDYSLTFGAINQTWSYRIHQNITYQVCRHAPRHPSFPTTQQLNVDRVFALYNDEERVLRFAVTNQIGPVKEDSDPTPGNPCYDGSHMCDTTARCHPGTGVDYTCECASGYQGDGRNCVDENECATGFHRCGPNSVCINLPGSYRCECRSGYEFADDRHTCIYVDECSENRCHPAATCYNTPGSFSCRCQPGYYGDGFQCIPDSTSSLTPCEQQQRHAQAQYAYPGARFHIPQCDEQGNFLPLQCHGSTGFCWCVDPDGHEVPGTQTPPGSTPPHCGPSPEPTQRPPTICERWRENLLEHYGGTPRDDQYVPQCDDLGHFIPLQCHGKSDFCWCVDKDGREVQGTRSQPGTTPACIPTVAPPMVRPTPRPDVTPPSVGTFLLYTQGQQIGYLPLNGTRLQKDAAKTLLSLHGSIIVGIDYDCRERMVYWTDVAGRTISRAGLELGAEPETIVNSGLISPEGLAIDHIRRTMYWTDSVLDKIESALLDGSERKVLFYTDLVNPRAIAVDPIRGNLYWTDWNREAPKIETSSLDGENRRILINTDIGLPNGLTFDPFSKLLCWADAGTKKLECTLPDGTGRRVIQNNLKYPFSIVSYADHFYHTDWRRDGVVSVNKHSGQFTDEYLPEQRSHLYGITAVYPYCPTGRK

Target ORF information:

RefSeq Version	XM_005267406.5
Organism	Homo sapiens(human)
Definition	Homo sapiens nidogen 2 (NID2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005267406.5

ORF Insert Sequence:

ATGGAGGGGG ACCGGGTGGC CGGGCGGCCG GTGCTGTCGT CGTTACCAGT GCTACTGCTG 
CTGCCGTTGC TAATGTTGCG GGCCGCGGCG CTGCACCCAG ACGAGCTCTT CCCACACGGG 
GAGTCGTGGG GGGACCAGCT CCTGCAGGAA GGCGACGACG AAAGCTCAGC CGTGGTGAAG 
CTGGCGAATC CCCTGCACTT CTACGAAGCC CGATTCAGCA ACCTCTACGT GGGCACCAAC 
GGCATCATCT CCACTCAGGA CTTCCCCAGG GAAACGCAGT ATGTGGACTA TGATTTCCCC 
ACCGACTTCC CGGCCATCGC CCCTTTTCTG GCGGACATCG ACACGAGCCA CGGCAGAGGC 
CGAGTCCTGT ACCGAGAGGA CACCTCCCCC GCAGTGCTGG GCCTGGCCGC CCGCTATGTG 
CGCGCTGGCT TCCCGCGCTC TGCGCGCTTT ACCCCCACCC ACGCCTTCCT GGCCACCTGG 
GAGCAGGTAG GCGCTTACGA GGAGGTCAAG CGCGGGGCGC TGCCCTCGGG AGAGCTGAAC 
ACTTTCCAGG CAGTTTTGGC ATCTGATGGG TCTGATAGCT ACGCCCTCTT TCTTTATCCT 
GCCAACGGCC TGCAGTTCCT TGGAACCCGC CCCAAAGAGT CTTACAATGT CCAGCTTCAG 
CTTCCAGCTC GGGTGGGCTT CTGCCGAGGG GAGGCTGATG ATCTGAAGTC AGAAGGACCA 
TATTTCAGCT TGACTAGCAC TGAACAGTCT GTGAAAAATC TCTATCAACT AAGCAACCTG 
GGGATCCCTG GAGTGTGGGC TTTCCATATC GGCAGCACTT CCCCGTTGGA CAATGTCAGG 
CCAGCTGCAG TTGGAGACCT TTCCGCTGCC CACTCTTCTG TTCCCCTGGG ACGTTCCTTC 
AGCCATGCTA CAGCCCTGGA AAGTGACTAT AATGAGGACA ATTTGGATTA CTATGATGTG 
AATGAGGAGG AAGCTGAATA CCTTCCGGGT GAACCAGAGG AGGCATTGAA TGGCCACAGC 
AGCATTGATG TTTCCTTCCA ATCCAAAGTG GATACAAAGC CTTTAGAGGG TAGGATCTCC 
CCTCCAGATT CTGATCTGTC CTCCCCCTTG CATCCAACAC CTACTTATTG GCCATTCTAT 
CCTGAAACAG AATCTTCCAC CTTGGATCCT CACACCAAAG AAGGAACATC TCTGGGAGAG 
GTAGGGGGCC CAGATTTAAA AGGCCAAGTT GAGCCCTGGG ATGAGAGAGA GACCAGAAGC 
CCAGCTCCAC CAGAGGTAGA CAGAGATTCA CTGGCTCCTT CCTGGGAAAC CCCACCACCG 
TACCCCGAAA ACGGAAGCAT CCAGCCCTAC CCAGATGGAG GGCCAGTGCC TTCGGAAATG 
GATGTTCCCC CAGCTCATCC TGAAGAAGAA ATTGTTCTTC GAAGTTACCC TGCTTCAGGT 
CACACTACAC CCTTAAGTCG AGGGACGTAT GAGGTGGGAC TGGAAGACAA CATAGGTTCC 
AACACCGAGG TCTTCACGTA TAATGCTGCC AACAAGGAAA CCTGTGAACA CAACCACAGA 
CAATGCTCCC GGCATGCCTT CTGCACGGAC TATGCCACTG GCTTCTGCTG CCACTGCCAA 
TCCAAGTTTT ATGGAAATGG GAAGCACTGT CTGCCTGAAG GGGCACCTCA CCGAGTGAAT 
GGGAAAGTGA GTGGCCACCT CCACGTGGGC CATACACCCG TGCACTTCAC TGATGTGGAC 
CTGCATGCGT ATATCGTGGG CAATGATGGC AGAGCCTACA CGGCCATCAG CCACATCCCA 
CAGCCAGCAG CCCAGGCCCT CCTCCCCCTC ACACCAATTG GAGGCCTGTT TGGCTGGCTC 
TTTGCTTTAG AAAAACCTGG CTCTGAGAAC GGCTTCAGCC TCGCAGGTGC TGCCTTTACC 
CATGACATGG AAGTTACATT CTACCCGGGA GAGGAGACGG TTCGTATCAC TCAAACTGCT 
GAGGGACTTG ACCCAGAGAA CTACCTGAGC ATTAAGACCA ACATTCAAGG CCAGGTGCCT 
TACGTCTCAG CAAATTTCAC AGCCCACATC TCTCCCTACA AGGAGCTGTA CCACTACTCC 
GACTCCACTG TGACCTCTAC AAGTTCCAGA GACTACTCTC TGACTTTTGG TGCAATCAAC 
CAAACATGGT CCTACCGCAT CCACCAGAAC ATCACTTACC AGGTGTGCAG GCACGCCCCC 
AGACACCCGT CCTTCCCCAC CACCCAGCAG CTGAACGTGG ACCGGGTCTT TGCCTTGTAT 
AATGACGAAG AAAGAGTGCT TAGATTTGCT GTGACCAATC AAATTGGCCC GGTCAAAGAG 
GATTCAGACC CCACTCCGGG GAATCCTTGC TATGATGGGA GCCACATGTG TGACACAACA 
GCACGGTGCC ATCCAGGGAC AGGTGTAGAT TACACCTGTG AGTGCGCATC TGGGTACCAG 
GGAGATGGAC GGAACTGTGT GGATGAAAAT GAATGTGCAA CTGGCTTTCA TCGCTGTGGC 
CCCAACTCTG TATGTATCAA CTTGCCTGGA AGCTACAGGT GTGAGTGCCG GAGTGGTTAT 
GAGTTTGCAG ATGACCGGCA TACTTGCATC TATGTAGATG AATGCTCAGA AAACAGATGT 
CACCCTGCAG CTACCTGCTA CAATACTCCT GGTTCCTTCT CCTGCCGTTG TCAACCCGGA 
TATTATGGGG ATGGATTTCA GTGCATACCT GACTCCACCT CAAGCCTGAC ACCCTGTGAA 
CAACAGCAGC GCCATGCCCA GGCCCAGTAT GCCTACCCTG GGGCCCGGTT CCACATCCCC 
CAATGCGACG AGCAGGGCAA CTTCCTGCCC CTACAGTGTC ATGGCAGCAC TGGTTTCTGC 
TGGTGCGTGG ACCCTGATGG TCATGAAGTT CCTGGTACCC AGACTCCACC TGGCTCCACC 
CCGCCTCACT GTGGACCATC ACCAGAGCCC ACCCAGAGGC CCCCGACCAT CTGTGAGCGC 
TGGAGGGAAA ACCTGCTGGA GCACTACGGT GGCACCCCCC GGGATGACCA GTACGTGCCC 
CAGTGCGATG ACCTGGGCCA CTTCATCCCC CTGCAGTGCC ACGGAAAGAG CGACTTCTGC 
TGGTGTGTGG ACAAAGATGG CAGAGAGGTG CAGGGCACCC GCTCCCAGCC AGGCACCACC 
CCTGCGTGTA TACCCACCGT CGCTCCACCC ATGGTCCGGC CCACGCCCCG GCCAGATGTG 
ACCCCTCCAT CTGTGGGCAC CTTCCTGCTC TATACTCAGG GCCAGCAGAT TGGCTACTTA 
CCCCTCAATG GCACCAGGCT TCAGAAGGAT GCAGCTAAGA CCCTGCTGTC TCTGCATGGC 
TCCATAATCG TGGGAATTGA TTACGACTGC CGGGAGAGGA TGGTGTACTG GACAGATGTT 
GCTGGACGGA CAATCAGCCG TGCTGGTCTG GAACTGGGAG CAGAGCCTGA GACGATCGTG 
AATTCAGGTC TGATAAGCCC TGAAGGACTT GCCATAGACC ACATCCGCAG AACAATGTAC 
TGGACGGACA GTGTCCTGGA TAAGATAGAG AGCGCCCTGC TGGATGGCTC TGAGCGCAAG 
GTCCTCTTCT ACACAGATCT GGTGAATCCC CGTGCCATCG CTGTGGATCC AATCCGAGGC 
AACTTGTACT GGACAGACTG GAATAGAGAA GCTCCTAAAA TTGAAACGTC ATCTTTAGAT 
GGAGAAAACA GAAGAATTCT GATCAATACA GACATTGGAT TGCCCAATGG CTTAACCTTT 
GACCCTTTCT CTAAACTGCT CTGCTGGGCA GATGCAGGAA CCAAAAAACT GGAGTGTACA 
CTACCTGATG GAACTGGACG GCGTGTCATT CAAAACAACC TCAAGTACCC CTTCAGCATC 
GTAAGCTATG CAGATCACTT CTACCACACA GACTGGAGGA GGGATGGTGT TGTATCAGTA 
AATAAACATA GTGGCCAGTT TACTGATGAG TATCTCCCAG AACAACGATC TCACCTCTAC 
GGGATAACTG CAGTCTACCC CTACTGCCCA ACAGGAAGAA AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Elevated levels of serum nidogen-2 in esophageal squamous cell carcinoma.
Cancer biomarkers : section A of Disease markers(2017 Dec)
Chai AWY,Cheung AKL,Dai W,Ko JMY,Lee NPY,Chan KT,Law SY,Lung ML

Characterization of the Extracellular Matrix of Normal and Diseased Tissues Using Proteomics.
Journal of proteome research16(8)3083-3091(2017 Aug)
Naba A,Pearce OMT,Del Rosario A,Ma D,Ding H,Rajeeve V,Cutillas PR,Balkwill FR,Hynes RO

Comprehensive proteomic characterization of stem cell-derived extracellular matrices.
Biomaterials128147-159(2017 06)
Ragelle H,Naba A,Larson BL,Zhou F,Priji? M,Whittaker CA,Del Rosario A,Langer R,Hynes RO,Anderson DG

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NID2 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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