FH cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	FH
Entrez Gene ID	2271
Full Name	fumarate hydratase
Synonyms	FMRD,HLRCC,LRCC,MCL,MCUL1
General protein information	Preferred Names fumarate hydratase Names fumarate hydratase, mitochondrial fumarase
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 1 Map Location: 1q43
Summary	The protein encoded by this gene is an enzymatic component of the tricarboxylic acid (TCA) cycle, or Krebs cycle, and catalyzes the formation of L-malate from fumarate. It exists in both a cytosolic form and an N-terminal extended form, differing only in the translation start site used. The N-terminal extended form is targeted to the mitochondrion, where the removal of the extension generates the same form as in the cytoplasm. It is similar to some thermostable class II fumarases and functions as a homotetramer. Mutations in this gene can cause fumarase deficiency and lead to progressive encephalopathy. [provided by RefSeq, Jul 2008].
Disorder MIM:	136850
Disorder Html:	Fumarase deficiency, 606812 (3); Leiomyomatosis and renal cell cancer, 150800 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_000143.3	NP_000134.2	fumarate hydratase, mitochondrial
XM_011544132.2	XP_011542434.1	fumarate hydratase, mitochondrial isoform X1
NM_000143.4	NP_000134.2	fumarate hydratase, mitochondrial

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	FH	XP_537215.1
Mus musculus (house mouse)	Fh1	NP_034339.2
Danio rerio (zebrafish)	fh	NP_957257.1
Drosophila melanogaster (fruit fly)	l(1)G0255	NP_572339.1
Arabidopsis thaliana (thale cress)	FUM2	NP_199908.1
Xenopus tropicalis (tropical clawed frog)	LOC100491219	XP_002935862.2
	FH	XP_001094968.1
Magnaporthe oryzae (rice blast fungus)	MGG_17962	XP_003720956.1
Pan troglodytes (chimpanzee)	FH	XP_001159979.1
Rattus norvegicus (Norway rat)	Fh	NP_058701.2
Saccharomyces cerevisiae (baker's yeast)	FUM1	NP_015061.1
Kluyveromyces lactis	KLLA0D00902g	XP_453112.1
Eremothecium gossypii	AGOS_ACR013C	NP_983416.2
Schizosaccharomyces pombe (fission yeast)	fum1	NP_588397.3
Arabidopsis thaliana (thale cress)	FUM1	NP_001078075.1
Homo sapiens (human)	FH	NP_000134.2
Bos taurus (cattle)	FH	NP_001069271.1
Gallus gallus (chicken)	FH	NP_001006382.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001884	XP_321179.4
Caenorhabditis elegans (roundworm)	fum-1	NP_498642.1
Neurospora crassa	NCU10008	XP_959454.1

Gene Ontology

The following FH gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the FH cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu18652	NM_000143.4 Latest version!	Homo sapiens fumarate hydratase (FH), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu57781	XM_011544132.2 Latest version!	Homo sapiens fumarate hydratase (FH), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00
View Old Accession Versions >>
OHu18652	NM_000143.3	Homo sapiens fumarate hydratase (FH), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

FH ( NM_000143.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_000134.2 Homo sapiens fumarate hydratase (FH), mRNA.

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CloneID	OHu18652
Clone ID Related Accession (Same CDS sequence)	NM_000143.3 , NM_000143.4
Accession Version	NM_000143.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1533bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-04
Organism	Homo sapiens(human)
Product	fumarate hydratase, mitochondrial
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA129148.1, BC003108.1, AA669797.1 and AW611924.1. This sequence is a reference standard in the RefSeqGene project. On Jun 26, 2010 this sequence version replaced NM_000143.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.200927.1, SRR1803614.170234.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGTACCGAG CACTTCGGCT CCTCGCGCGC TCGCGTCCCC TCGTGCGGGC TCCAGCCGCA 
GCCTTAGCTT CGGCTCCCGG CTTGGGTGGC GCGGCCGTGC CCTCGTTTTG GCCTCCGAAC 
GCGGCTCGAA TGGCAAGCCA AAATTCCTTC CGGATAGAAT ATGATACCTT TGGTGAACTA 
AAGGTGCCAA ATGATAAGTA TTATGGCGCC CAGACCGTGA GATCTACGAT GAACTTTAAG 
ATTGGAGGTG TGACAGAACG CATGCCAACC CCAGTTATTA AAGCTTTTGG CATCTTGAAG 
CGAGCGGCCG CTGAAGTAAA CCAGGATTAT GGTCTTGATC CAAAGATTGC TAATGCAATA 
ATGAAGGCAG CAGATGAGGT AGCTGAAGGT AAATTAAATG ATCATTTTCC TCTCGTGGTA 
TGGCAGACTG GATCAGGAAC TCAGACAAAT ATGAATGTAA ATGAAGTCAT TAGCAATAGA 
GCAATTGAAA TGTTAGGAGG TGAACTTGGC AGCAAGATAC CTGTGCATCC CAACGATCAT 
GTTAATAAAA GCCAGAGCTC AAATGATACT TTTCCCACAG CAATGCACAT TGCTGCTGCA 
ATAGAAGTTC ATGAAGTACT GTTACCAGGA CTACAGAAGT TACATGATGC TCTTGATGCA 
AAATCCAAAG AGTTTGCACA GATCATCAAG ATTGGACGTA CTCATACTCA GGATGCTGTT 
CCACTTACTC TTGGGCAGGA ATTTAGTGGT TATGTTCAAC AAGTAAAATA TGCAATGACA 
AGAATAAAAG CTGCCATGCC AAGAATCTAT GAGCTCGCAG CTGGAGGCAC TGCTGTTGGT 
ACAGGTTTAA ATACTAGAAT TGGCTTTGCA GAAAAGGTTG CTGCAAAAGT GGCTGCACTT 
ACAGGCTTGC CTTTTGTCAC TGCTCCGAAT AAATTTGAAG CTCTGGCTGC TCATGACGCT 
CTGGTTGAGC TCAGTGGAGC CATGAACACT ACTGCCTGCA GTCTGATGAA GATAGCAAAT 
GATATTCGAT TTTTGGGTTC TGGTCCTCGG TCAGGTCTGG GAGAATTGAT CTTGCCTGAA 
AATGAACCAG GAAGCAGTAT CATGCCAGGC AAGGTGAACC CTACTCAGTG TGAAGCAATG 
ACCATGGTTG CAGCCCAAGT CATGGGGAAC CATGTTGCTG TCACTGTCGG AGGCAGCAAT 
GGACATTTTG AGTTGAATGT TTTCAAGCCA ATGATGATTA AAAATGTGTT ACACTCAGCC 
AGGCTGCTGG GGGATGCTTC AGTTTCCTTT ACAGAAAACT GCGTGGTGGG AATCCAGGCC 
AATACAGAAA GGATCAACAA GCTGATGAAT GAGTCTCTAA TGTTGGTGAC AGCTCTCAAT 
CCTCATATAG GGTATGACAA GGCAGCAAAG ATTGCTAAGA CAGCACACAA AAATGGATCA 
ACCTTAAAGG AAACTGCTAT CGAACTTGGC TATCTCACAG CAGAGCAGTT TGACGAATGG 
GTAAAACCTA AGGACATGCT GGGTCCAAAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000134.2
CDS	64..1596
Translation	MYRALRLLARSRPLVRAPAAALASAPGLGGAAVPSFWPPNAARMASQNSFRIEYDTFGELKVPNDKYYGAQTVRSTMNFKIGGVTERMPTPVIKAFGILKRAAAEVNQDYGLDPKIANAIMKAADEVAEGKLNDHFPLVVWQTGSGTQTNMNVNEVISNRAIEMLGGELGSKIPVHPNDHVNKSQSSNDTFPTAMHIAAAIEVHEVLLPGLQKLHDALDAKSKEFAQIIKIGRTHTQDAVPLTLGQEFSGYVQQVKYAMTRIKAAMPRIYELAAGGTAVGTGLNTRIGFAEKVAAKVAALTGLPFVTAPNKFEALAAHDALVELSGAMNTTACSLMKIANDIRFLGSGPRSGLGELILPENEPGSSIMPGKVNPTQCEAMTMVAAQVMGNHVAVTVGGSNGHFELNVFKPMMIKNVLHSARLLGDASVSFTENCVVGIQANTERINKLMNESLMLVTALNPHIGYDKAAKIAKTAHKNGSTLKETAIELGYLTAEQFDEWVKPKDMLGPK

Target ORF information:

RefSeq Version	NM_000143.3
Organism	Homo sapiens(human)
Definition	Homo sapiens fumarate hydratase (FH), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000143.3

ORF Insert Sequence:

ATGTACCGAG CACTTCGGCT CCTCGCGCGC TCGCGTCCCC TCGTGCGGGC TCCAGCCGCA 
GCCTTAGCTT CGGCTCCCGG CTTGGGTGGC GCGGCCGTGC CCTCGTTTTG GCCTCCGAAC 
GCGGCTCGAA TGGCAAGCCA AAATTCCTTC CGGATAGAAT ATGATACCTT TGGTGAACTA 
AAGGTGCCAA ATGATAAGTA TTATGGCGCC CAGACCGTGA GATCTACGAT GAACTTTAAG 
ATTGGAGGTG TGACAGAACG CATGCCAACC CCAGTTATTA AAGCTTTTGG CATCTTGAAG 
CGAGCGGCCG CTGAAGTAAA CCAGGATTAT GGTCTTGATC CAAAGATTGC TAATGCAATA 
ATGAAGGCAG CAGATGAGGT AGCTGAAGGT AAATTAAATG ATCATTTTCC TCTCGTGGTA 
TGGCAGACTG GATCAGGAAC TCAGACAAAT ATGAATGTAA ATGAAGTCAT TAGCAATAGA 
GCAATTGAAA TGTTAGGAGG TGAACTTGGC AGCAAGATAC CTGTGCATCC CAACGATCAT 
GTTAATAAAA GCCAGAGCTC AAATGATACT TTTCCCACAG CAATGCACAT TGCTGCTGCA 
ATAGAAGTTC ATGAAGTACT GTTACCAGGA CTACAGAAGT TACATGATGC TCTTGATGCA 
AAATCCAAAG AGTTTGCACA GATCATCAAG ATTGGACGTA CTCATACTCA GGATGCTGTT 
CCACTTACTC TTGGGCAGGA ATTTAGTGGT TATGTTCAAC AAGTAAAATA TGCAATGACA 
AGAATAAAAG CTGCCATGCC AAGAATCTAT GAGCTCGCAG CTGGAGGCAC TGCTGTTGGT 
ACAGGTTTAA ATACTAGAAT TGGCTTTGCA GAAAAGGTTG CTGCAAAAGT GGCTGCACTT 
ACAGGCTTGC CTTTTGTCAC TGCTCCGAAT AAATTTGAAG CTCTGGCTGC TCATGACGCT 
CTGGTTGAGC TCAGTGGAGC CATGAACACT ACTGCCTGCA GTCTGATGAA GATAGCAAAT 
GATATTCGAT TTTTGGGTTC TGGTCCTCGG TCAGGTCTGG GAGAATTGAT CTTGCCTGAA 
AATGAACCAG GAAGCAGTAT CATGCCAGGC AAGGTGAACC CTACTCAGTG TGAAGCAATG 
ACCATGGTTG CAGCCCAAGT CATGGGGAAC CATGTTGCTG TCACTGTCGG AGGCAGCAAT 
GGACATTTTG AGTTGAATGT TTTCAAGCCA ATGATGATTA AAAATGTGTT ACACTCAGCC 
AGGCTGCTGG GGGATGCTTC AGTTTCCTTT ACAGAAAACT GCGTGGTGGG AATCCAGGCC 
AATACAGAAA GGATCAACAA GCTGATGAAT GAGTCTCTAA TGTTGGTGAC AGCTCTCAAT 
CCTCATATAG GGTATGACAA GGCAGCAAAG ATTGCTAAGA CAGCACACAA AAATGGATCA 
ACCTTAAAGG AAACTGCTAT CGAACTTGGC TATCTCACAG CAGAGCAGTT TGACGAATGG 
GTAAAACCTA AGGACATGCT GGGTCCAAAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FH ( NM_000143.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_000134.2 Homo sapiens fumarate hydratase (FH), mRNA.

Price & Availability↑

CloneID	OHu18652
Clone ID Related Accession (Same CDS sequence)	NM_000143.3 , NM_000143.4
Accession Version	NM_000143.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1533bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-04
Organism	Homo sapiens(human)
Product	fumarate hydratase, mitochondrial
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA129148.1, BC003108.1 and AA669797.1. On Nov 30, 2019 this sequence version replaced NM_000143.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U59309.1, BC003108.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta RefSeq Select criteria :: based on manual assertion, conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGTACCGAG CACTTCGGCT CCTCGCGCGC TCGCGTCCCC TCGTGCGGGC TCCAGCCGCA 
GCCTTAGCTT CGGCTCCCGG CTTGGGTGGC GCGGCCGTGC CCTCGTTTTG GCCTCCGAAC 
GCGGCTCGAA TGGCAAGCCA AAATTCCTTC CGGATAGAAT ATGATACCTT TGGTGAACTA 
AAGGTGCCAA ATGATAAGTA TTATGGCGCC CAGACCGTGA GATCTACGAT GAACTTTAAG 
ATTGGAGGTG TGACAGAACG CATGCCAACC CCAGTTATTA AAGCTTTTGG CATCTTGAAG 
CGAGCGGCCG CTGAAGTAAA CCAGGATTAT GGTCTTGATC CAAAGATTGC TAATGCAATA 
ATGAAGGCAG CAGATGAGGT AGCTGAAGGT AAATTAAATG ATCATTTTCC TCTCGTGGTA 
TGGCAGACTG GATCAGGAAC TCAGACAAAT ATGAATGTAA ATGAAGTCAT TAGCAATAGA 
GCAATTGAAA TGTTAGGAGG TGAACTTGGC AGCAAGATAC CTGTGCATCC CAACGATCAT 
GTTAATAAAA GCCAGAGCTC AAATGATACT TTTCCCACAG CAATGCACAT TGCTGCTGCA 
ATAGAAGTTC ATGAAGTACT GTTACCAGGA CTACAGAAGT TACATGATGC TCTTGATGCA 
AAATCCAAAG AGTTTGCACA GATCATCAAG ATTGGACGTA CTCATACTCA GGATGCTGTT 
CCACTTACTC TTGGGCAGGA ATTTAGTGGT TATGTTCAAC AAGTAAAATA TGCAATGACA 
AGAATAAAAG CTGCCATGCC AAGAATCTAT GAGCTCGCAG CTGGAGGCAC TGCTGTTGGT 
ACAGGTTTAA ATACTAGAAT TGGCTTTGCA GAAAAGGTTG CTGCAAAAGT GGCTGCACTT 
ACAGGCTTGC CTTTTGTCAC TGCTCCGAAT AAATTTGAAG CTCTGGCTGC TCATGACGCT 
CTGGTTGAGC TCAGTGGAGC CATGAACACT ACTGCCTGCA GTCTGATGAA GATAGCAAAT 
GATATTCGAT TTTTGGGTTC TGGTCCTCGG TCAGGTCTGG GAGAATTGAT CTTGCCTGAA 
AATGAACCAG GAAGCAGTAT CATGCCAGGC AAGGTGAACC CTACTCAGTG TGAAGCAATG 
ACCATGGTTG CAGCCCAAGT CATGGGGAAC CATGTTGCTG TCACTGTCGG AGGCAGCAAT 
GGACATTTTG AGTTGAATGT TTTCAAGCCA ATGATGATTA AAAATGTGTT ACACTCAGCC 
AGGCTGCTGG GGGATGCTTC AGTTTCCTTT ACAGAAAACT GCGTGGTGGG AATCCAGGCC 
AATACAGAAA GGATCAACAA GCTGATGAAT GAGTCTCTAA TGTTGGTGAC AGCTCTCAAT 
CCTCATATAG GGTATGACAA GGCAGCAAAG ATTGCTAAGA CAGCACACAA AAATGGATCA 
ACCTTAAAGG AAACTGCTAT CGAACTTGGC TATCTCACAG CAGAGCAGTT TGACGAATGG 
GTAAAACCTA AGGACATGCT GGGTCCAAAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000134.2
CDS	34..1566
Translation	MYRALRLLARSRPLVRAPAAALASAPGLGGAAVPSFWPPNAARMASQNSFRIEYDTFGELKVPNDKYYGAQTVRSTMNFKIGGVTERMPTPVIKAFGILKRAAAEVNQDYGLDPKIANAIMKAADEVAEGKLNDHFPLVVWQTGSGTQTNMNVNEVISNRAIEMLGGELGSKIPVHPNDHVNKSQSSNDTFPTAMHIAAAIEVHEVLLPGLQKLHDALDAKSKEFAQIIKIGRTHTQDAVPLTLGQEFSGYVQQVKYAMTRIKAAMPRIYELAAGGTAVGTGLNTRIGFAEKVAAKVAALTGLPFVTAPNKFEALAAHDALVELSGAMNTTACSLMKIANDIRFLGSGPRSGLGELILPENEPGSSIMPGKVNPTQCEAMTMVAAQVMGNHVAVTVGGSNGHFELNVFKPMMIKNVLHSARLLGDASVSFTENCVVGIQANTERINKLMNESLMLVTALNPHIGYDKAAKIAKTAHKNGSTLKETAIELGYLTAEQFDEWVKPKDMLGPK

Target ORF information:

RefSeq Version	NM_000143.4
Organism	Homo sapiens(human)
Definition	Homo sapiens fumarate hydratase (FH), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000143.4

ORF Insert Sequence:

ATGTACCGAG CACTTCGGCT CCTCGCGCGC TCGCGTCCCC TCGTGCGGGC TCCAGCCGCA 
GCCTTAGCTT CGGCTCCCGG CTTGGGTGGC GCGGCCGTGC CCTCGTTTTG GCCTCCGAAC 
GCGGCTCGAA TGGCAAGCCA AAATTCCTTC CGGATAGAAT ATGATACCTT TGGTGAACTA 
AAGGTGCCAA ATGATAAGTA TTATGGCGCC CAGACCGTGA GATCTACGAT GAACTTTAAG 
ATTGGAGGTG TGACAGAACG CATGCCAACC CCAGTTATTA AAGCTTTTGG CATCTTGAAG 
CGAGCGGCCG CTGAAGTAAA CCAGGATTAT GGTCTTGATC CAAAGATTGC TAATGCAATA 
ATGAAGGCAG CAGATGAGGT AGCTGAAGGT AAATTAAATG ATCATTTTCC TCTCGTGGTA 
TGGCAGACTG GATCAGGAAC TCAGACAAAT ATGAATGTAA ATGAAGTCAT TAGCAATAGA 
GCAATTGAAA TGTTAGGAGG TGAACTTGGC AGCAAGATAC CTGTGCATCC CAACGATCAT 
GTTAATAAAA GCCAGAGCTC AAATGATACT TTTCCCACAG CAATGCACAT TGCTGCTGCA 
ATAGAAGTTC ATGAAGTACT GTTACCAGGA CTACAGAAGT TACATGATGC TCTTGATGCA 
AAATCCAAAG AGTTTGCACA GATCATCAAG ATTGGACGTA CTCATACTCA GGATGCTGTT 
CCACTTACTC TTGGGCAGGA ATTTAGTGGT TATGTTCAAC AAGTAAAATA TGCAATGACA 
AGAATAAAAG CTGCCATGCC AAGAATCTAT GAGCTCGCAG CTGGAGGCAC TGCTGTTGGT 
ACAGGTTTAA ATACTAGAAT TGGCTTTGCA GAAAAGGTTG CTGCAAAAGT GGCTGCACTT 
ACAGGCTTGC CTTTTGTCAC TGCTCCGAAT AAATTTGAAG CTCTGGCTGC TCATGACGCT 
CTGGTTGAGC TCAGTGGAGC CATGAACACT ACTGCCTGCA GTCTGATGAA GATAGCAAAT 
GATATTCGAT TTTTGGGTTC TGGTCCTCGG TCAGGTCTGG GAGAATTGAT CTTGCCTGAA 
AATGAACCAG GAAGCAGTAT CATGCCAGGC AAGGTGAACC CTACTCAGTG TGAAGCAATG 
ACCATGGTTG CAGCCCAAGT CATGGGGAAC CATGTTGCTG TCACTGTCGG AGGCAGCAAT 
GGACATTTTG AGTTGAATGT TTTCAAGCCA ATGATGATTA AAAATGTGTT ACACTCAGCC 
AGGCTGCTGG GGGATGCTTC AGTTTCCTTT ACAGAAAACT GCGTGGTGGG AATCCAGGCC 
AATACAGAAA GGATCAACAA GCTGATGAAT GAGTCTCTAA TGTTGGTGAC AGCTCTCAAT 
CCTCATATAG GGTATGACAA GGCAGCAAAG ATTGCTAAGA CAGCACACAA AAATGGATCA 
ACCTTAAAGG AAACTGCTAT CGAACTTGGC TATCTCACAG CAGAGCAGTT TGACGAATGG 
GTAAAACCTA AGGACATGCT GGGTCCAAAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FH ( XM_011544132.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011542434.1 Homo sapiens fumarate hydratase (FH), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu57781
Clone ID Related Accession (Same CDS sequence)	XM_011544132.2
Accession Version	XM_011544132.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1305bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	fumarate hydratase, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011544132.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACTTTA AGATTGGAGG TGTGACAGAA CGCATGCCAA CCCCAGTTAT TAAAGCTTTT 
GGCATCTTGA AGCGAGCGGC CGCTGAAGTA AACCAGGATT ATGGTCTTGA TCCAAAGATT 
GCTAATGCAA TAATGAAGGC AGCAGATGAG GTAGCTGAAG GTAAATTAAA TGATCATTTT 
CCTCTCGTGG TATGGCAGAC TGGATCAGGA ACTCAGACAA ATATGAATGT AAATGAAGTC 
ATTAGCAATA GAGCAATTGA AATGTTAGGA GGTGAACTTG GCAGCAAGAT ACCTGTGCAT 
CCCAACGATC ATGTTAATAA AAGCCAGAGC TCAAATGATA CTTTTCCCAC AGCAATGCAC 
ATTGCTGCTG CAATAGAAGT TCATGAAGTA CTGTTACCAG GACTACAGAA GTTACATGAT 
GCTCTTGATG CAAAATCCAA AGAGTTTGCA CAGATCATCA AGATTGGACG TACTCATACT 
CAGGATGCTG TTCCACTTAC TCTTGGGCAG GAATTTAGTG GTTATGTTCA ACAAGTAAAA 
TATGCAATGA CAAGAATAAA AGCTGCCATG CCAAGAATCT ATGAGCTCGC AGCTGGAGGC 
ACTGCTGTTG GTACAGGTTT AAATACTAGA ATTGGCTTTG CAGAAAAGGT TGCTGCAAAA 
GTGGCTGCAC TTACAGGCTT GCCTTTTGTC ACTGCTCCGA ATAAATTTGA AGCTCTGGCT 
GCTCATGACG CTCTGGTTGA GCTCAGTGGA GCCATGAACA CTACTGCCTG CAGTCTGATG 
AAGATAGCAA ATGATATTCG ATTTTTGGGT TCTGGTCCTC GGTCAGGTCT GGGAGAATTG 
ATCTTGCCTG AAAATGAACC AGGAAGCAGT ATCATGCCAG GCAAGGTGAA CCCTACTCAG 
TGTGAAGCAA TGACCATGGT TGCAGCCCAA GTCATGGGGA ACCATGTTGC TGTCACTGTC 
GGAGGCAGCA ATGGACATTT TGAGTTGAAT GTTTTCAAGC CAATGATGAT TAAAAATGTG 
TTACACTCAG CCAGGCTGCT GGGGGATGCT TCAGTTTCCT TTACAGAAAA CTGCGTGGTG 
GGAATCCAGG CCAATACAGA AAGGATCAAC AAGCTGATGA ATGAGTCTCT AATGTTGGTG 
ACAGCTCTCA ATCCTCATAT AGGGTATGAC AAGGCAGCAA AGATTGCTAA GACAGCACAC 
AAAAATGGAT CAACCTTAAA GGAAACTGCT ATCGAACTTG GCTATCTCAC AGCAGAGCAG 
TTTGACGAAT GGGTAAAACC TAAGGACATG CTGGGTCCAA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011542434.1
CDS	732..2036
Translation	MNFKIGGVTERMPTPVIKAFGILKRAAAEVNQDYGLDPKIANAIMKAADEVAEGKLNDHFPLVVWQTGSGTQTNMNVNEVISNRAIEMLGGELGSKIPVHPNDHVNKSQSSNDTFPTAMHIAAAIEVHEVLLPGLQKLHDALDAKSKEFAQIIKIGRTHTQDAVPLTLGQEFSGYVQQVKYAMTRIKAAMPRIYELAAGGTAVGTGLNTRIGFAEKVAAKVAALTGLPFVTAPNKFEALAAHDALVELSGAMNTTACSLMKIANDIRFLGSGPRSGLGELILPENEPGSSIMPGKVNPTQCEAMTMVAAQVMGNHVAVTVGGSNGHFELNVFKPMMIKNVLHSARLLGDASVSFTENCVVGIQANTERINKLMNESLMLVTALNPHIGYDKAAKIAKTAHKNGSTLKETAIELGYLTAEQFDEWVKPKDMLGPK

Target ORF information:

RefSeq Version	XM_011544132.2
Organism	Homo sapiens(human)
Definition	Homo sapiens fumarate hydratase (FH), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011544132.2

ORF Insert Sequence:

ATGAACTTTA AGATTGGAGG TGTGACAGAA CGCATGCCAA CCCCAGTTAT TAAAGCTTTT 
GGCATCTTGA AGCGAGCGGC CGCTGAAGTA AACCAGGATT ATGGTCTTGA TCCAAAGATT 
GCTAATGCAA TAATGAAGGC AGCAGATGAG GTAGCTGAAG GTAAATTAAA TGATCATTTT 
CCTCTCGTGG TATGGCAGAC TGGATCAGGA ACTCAGACAA ATATGAATGT AAATGAAGTC 
ATTAGCAATA GAGCAATTGA AATGTTAGGA GGTGAACTTG GCAGCAAGAT ACCTGTGCAT 
CCCAACGATC ATGTTAATAA AAGCCAGAGC TCAAATGATA CTTTTCCCAC AGCAATGCAC 
ATTGCTGCTG CAATAGAAGT TCATGAAGTA CTGTTACCAG GACTACAGAA GTTACATGAT 
GCTCTTGATG CAAAATCCAA AGAGTTTGCA CAGATCATCA AGATTGGACG TACTCATACT 
CAGGATGCTG TTCCACTTAC TCTTGGGCAG GAATTTAGTG GTTATGTTCA ACAAGTAAAA 
TATGCAATGA CAAGAATAAA AGCTGCCATG CCAAGAATCT ATGAGCTCGC AGCTGGAGGC 
ACTGCTGTTG GTACAGGTTT AAATACTAGA ATTGGCTTTG CAGAAAAGGT TGCTGCAAAA 
GTGGCTGCAC TTACAGGCTT GCCTTTTGTC ACTGCTCCGA ATAAATTTGA AGCTCTGGCT 
GCTCATGACG CTCTGGTTGA GCTCAGTGGA GCCATGAACA CTACTGCCTG CAGTCTGATG 
AAGATAGCAA ATGATATTCG ATTTTTGGGT TCTGGTCCTC GGTCAGGTCT GGGAGAATTG 
ATCTTGCCTG AAAATGAACC AGGAAGCAGT ATCATGCCAG GCAAGGTGAA CCCTACTCAG 
TGTGAAGCAA TGACCATGGT TGCAGCCCAA GTCATGGGGA ACCATGTTGC TGTCACTGTC 
GGAGGCAGCA ATGGACATTT TGAGTTGAAT GTTTTCAAGC CAATGATGAT TAAAAATGTG 
TTACACTCAG CCAGGCTGCT GGGGGATGCT TCAGTTTCCT TTACAGAAAA CTGCGTGGTG 
GGAATCCAGG CCAATACAGA AAGGATCAAC AAGCTGATGA ATGAGTCTCT AATGTTGGTG 
ACAGCTCTCA ATCCTCATAT AGGGTATGAC AAGGCAGCAA AGATTGCTAA GACAGCACAC 
AAAAATGGAT CAACCTTAAA GGAAACTGCT ATCGAACTTG GCTATCTCAC AGCAGAGCAG 
TTTGACGAAT GGGTAAAACC TAAGGACATG CTGGGTCCAA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

見積依頼

Eメール

japanmarket@genscript.com

電話

03-6811-6572

FAX

03-6811-6573
050-3174-7589(受注専用)

オンラインフォーム

オンライン注文

FH cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

見積依頼

Eメール

電話

FAX

オンラインフォーム

サポート

会社情報

サポート

会社情報