LRWD1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	LRWD1
Entrez Gene ID	222229
Full Name	leucine rich repeats and WD repeat domain containing 1
Synonyms	CENP-33,ORCA
General protein information	Preferred Names leucine rich repeats and WD repeat domain containing 1 Names leucine-rich repeat and WD repeat-containing protein 1 ORC-associated protein centromere protein 33 origin recognition complex-associated protein testicular tissue protein Li 4
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 7 Map Location: 7q22.1
Summary	The protein encoded by this gene interacts with components of the origin recognition complex (ORC) and regulates the formation of the prereplicative complex. The encoded protein stabilizes the ORC and therefore aids in DNA replication. This protein is required for the G1/S phase transition of the cell cycle. In addition, the encoded protein binds to trimethylated histone H3 in heterochromatin and recruits the ORC and lysine methyltransferases, which help maintain the repressive heterochromatic state. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2015].
Disorder MIM:	615167

mRNA and Protein(s)

mRNA	Protein	Name
NM_152892.3	NP_690852.1	leucine-rich repeat and WD repeat-containing protein 1 isoform 1
NM_152892.2	NP_690852.1	leucine-rich repeat and WD repeat-containing protein 1 isoform 1
NM_152892.1	NP_690852.1	leucine-rich repeat and WD repeat-containing protein 1
XM_017011845.1	XP_016867334.1	leucine-rich repeat and WD repeat-containing protein 1 isoform X1
NM_001317721.1	NP_001304650.1	leucine-rich repeat and WD repeat-containing protein 1 isoform 2

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	LRWD1	XP_519556.3
	LRWD1	XP_001108941.1
Bos taurus (cattle)	LRWD1	NP_001178398.1
Gallus gallus (chicken)	LRWD1	XP_415759.2
Homo sapiens (human)	LRWD1	NP_690852.1
Mus musculus (house mouse)	Lrwd1	NP_082167.2
Canis lupus familiaris (dog)	LRWD1	XP_536851.3
Danio rerio (zebrafish)	lrwd1	XP_683313.4
Xenopus tropicalis (tropical clawed frog)	lrwd1	NP_001120130.1

Gene Ontology
Bibliography

Related articles in PubMed

LRWD1 Regulates Microtubule Nucleation and Proper Cell Cycle Progression in the Human Testicular Embryonic Carcinoma Cells.
Wang CY, Hong YH, Syu JS, Tsai YC, Liu XY, Chen TY, Su YM, Kuo PL, Lin YM, Teng YN
Journal of cellular biochemistry119(1)314-326(2018 Jan)

High-throughput RNAi screen in Ewing sarcoma cells identifies leucine rich repeats and WD repeat domain containing 1 (LRWD1) as a regulator of EWS-FLI1 driven cell viability.
He T, Surdez D, Rantala JK, Haapa-Paananen S, Ban J, Kauer M, Tomazou E, Fey V, Alonso J, Kovar H, Delattre O, Iljin K
Gene596137-146(2017 Jan)

The preRC protein ORCA organizes heterochromatin by assembling histone H3 lysine 9 methyltransferases on chromatin.
Giri S, Aggarwal V, Pontis J, Shen Z, Chakraborty A, Khan A, Mizzen C, Prasanth KV, Ait-Si-Ali S, Ha T, Prasanth SG
eLife4(2015 Apr)

Correlation between leucine rich domain and the stability of LRWD1 protein in human NT2/D1 cells.
Tsai YC, Teng YN, Hung JH, Wu CH, Kuo YT, Kuo PL, Chiu CC, Huang B
Advances in medical sciences59(2)266-72(2014 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

LRWD1 is a novel regulator of EWS-FLI1 driven cell proliferation in Ewing sarcoma cells. EWS-FLI1 regulates LRWD1 expression and LRWD1 may contribute to EWS-FLI1 mediated transcriptional repression.
Title: High-throughput RNAi screen in Ewing sarcoma cells identifies leucine rich repeats and WD repeat domain containing 1 (LRWD1) as a regulator of EWS-FLI1 driven cell viability.

Data show that ORCA acts as a scaffold for the establishment of H3K9 KMT complex.
Title: The preRC protein ORCA organizes heterochromatin by assembling histone H3 lysine 9 methyltransferases on chromatin.

Data (from studies using recombinant proteins) suggest that deletion of leucine rich (LRR) domain significantly down-regulates expression of LRWD1 protein; LRR domain is significantly correlated with stability of LRWD1 protein.
Title: Correlation between leucine rich domain and the stability of LRWD1 protein in human NT2/D1 cells.

three coding single-nucleotide polymorphisms (SNP1-SNP3) could be detected in Japanese patients with Sertoli cell-only syndrome
Title: Single-nucleotide polymorphisms in the LRWD1 gene may be a genetic risk factor for Japanese patients with Sertoli cell-only syndrome.

This is the first study showing that the expression of the testis-enriched LRWD1 gene is regulated by NF-kappaB.
Title: Nuclear factor-?B (NF-?B) regulates the expression of human testis-enriched Leucine-rich repeats and WD repeat domain containing 1 (LRWD1) gene.

The following LRWD1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LRWD1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu30993	NM_152892.3 Latest version!	Homo sapiens leucine rich repeats and WD repeat domain containing 1 (LRWD1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu41426	NM_001317721.1 Latest version!	Homo sapiens leucine rich repeats and WD repeat domain containing 1 (LRWD1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00
OHu97093	XM_017011845.1 Latest version!	Homo sapiens leucine-rich repeats and WD repeat domain containing 1 (LRWD1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
View Old Accession Versions >>
OHu30993	NM_152892.2	Homo sapiens leucine rich repeats and WD repeat domain containing 1 (LRWD1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu30993	NM_152892.1	Homo sapiens leucine-rich repeats and WD repeat domain containing 1 (LRWD1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LRWD1 ( NM_152892.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_690852.1 Homo sapiens leucine rich repeats and WD repeat domain containing 1 (LRWD1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu30993
Clone ID Related Accession (Same CDS sequence)	NM_152892.3 , NM_152892.2 , NM_152892.1
Accession Version	NM_152892.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1944bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-16
Organism	Homo sapiens(human)
Product	leucine-rich repeat and WD repeat-containing protein 1 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC030547.1 and BC009436.1. On Nov 22, 2018 this sequence version replaced NM_152892.2. Transcript Variant: This variant (1) encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AL133057.1, SRR1803614.4182.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000292616.10/ ENSP00000292616.5 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGGCCCCC TCTCGGCGCG GCTGCTAATG CAGCGCGGGC GCCCCAAGAG CGACCGGCTG 
GGGAAGATCC GGAGTCTGGA CCTGTCAGGA TTGGAGCTGC TTTCCGAGCA CCTGGACCCC 
AAACTCCTGT GCCGCCTGAC GCAGCTGCAG GAGCTTGACC TGTCTAACAA CCACCTGGAG 
ACGCTGCCGG ACAACCTGGG CCTGTCCCAC CTGCGTGTCC TCCGCTGCGC CAACAACCAG 
CTGGGGGATG TTACTGCCTT GTGCCAGTTC CCCAAGCTCG AGGAACTCAG CCTGGAGGGC 
AACCCCTTCC TGACGGTCAA TGACAACCTG AAAGTCTCCT TTCTCCTGCC CACGCTCCGT 
AAGGTCAATG GCAAGGATGC GTCCTCAACT TACTCTCAGG TGGAGAACCT GAATCGGGAG 
CTGACCAGCA GGGTCACAGC TCACTGGGAG AAGTTCATGG CCACACTGGG TCCTGAAGAG 
GAGGCTGAGA AGGCCCAGGC GGACTTTGTG AAGTCGGCTG TCAGGGATGT CCGCTACGGG 
CCCGAGTCCC TCAGCGAGTT CACCCAGTGG CGGGTGCGGA TGATCTCTGA GGAGCTGGTG 
GCCGCCAGTA GGACCCAGGT GCAAAAGGCT AACAGCCCAG AGAAGCCCCC AGAAGCTGGA 
GCTGCCCACA AGCCCAGGGC CAGACTGGCG GCCTTGAAAC GGCCAGACGA CGTCCCACTC 
AGCCTCTCTC CCAGCAAGCG GGCGTGTGCC TCCCCGTCGG CCCAGGTGGA GGGCAGCCCT 
GTGGCAGGCT CCGATGGCAG CCAGCCTGCT GTGAAGCTGG AGCCCCTGCA CTTCCTGCAG 
TGCCACAGCA AGAACAACAG CCCCCAGGAC CTCGAGACCC AGCTGTGGGC CTGTGCCTTC 
GAGCCGGCCT GGGAGGAGGG GGCCACATCC CAGACCGTGG CCACGTGCGG CGGGGAGGCT 
GTGTGCGTAA TTGATTGCCA GACGGGCATC GTGCTCCACA AGTACAAGGC ACCCGGCGAG 
GAGTTCTTTT CTGTGGCCTG GACCGCTCTG ATGGTGGTCA CACAGGCTGG CCACAAGAAG 
CGCTGGAGTG TGCTGGCGGC TGCAGGCCTA CGGGGCCTGG TCCGGCTGCT GCACGTGCGT 
GCCGGCTTCT GCTGCGGGGT CATCCGAGCC CACAAGAAGG CCATCGCCAC CCTGTGCTTC 
AGCCCCGCCC ACGAGACCCA TCTCTTCACG GCCTCCTATG ACAAGCGGAT CATCCTCTGG 
GACATCGGGG TGCCCAACCA GGACTACGAA TTCCAGGCCA GCCAGCTGCT CACACTGGAC 
ACCACCTCTA TCCCCCTGCG CCTCTGCCCT GTCGCCTCCT GCCCGGACGC CCGCCTGCTG 
GCCGGCTGCG AGGGCGGCTG CTGCTGCTGG GACGTGCGGC TGGACCAGCC CCAAAAGAGG 
AGGGTGTGTG AAGTGGAATT CGTCTTCTCT GAGGGCTCCG AGGCATCTGG ACGGAGAGTG 
GATGGGCTGG CATTTGTGAA TGAGGACATC GTGGCCTCCA AGGGGAGCGG CCTGGGCACC 
ATCTGCCTGT GGAGCTGGAG GCAGACGTGG GGGGGCCGGG GCAGCCAGTC CACGGTGGCA 
GTGGTGGTCC TGGCGCGGCT GCAATGGTCG TCCACCGAGT TGGCCTACTT CTCGCTCAGC 
GCCTGCCCTG ATAAGGGGAT TGTGCTCTGT GGGGATGAGG AGGGCAACGT GTGGCTCTAC 
GACGTCAGCA ACATCCTGAA GCAGCCACCC CTGCTGCCGG CAGCCCTGCA GGCCCCCACA 
CAGATCCTGA AGTGGCCCCA GCCCTGGGCC CTTGGCCAGG TGGTGACCAA GACCATGGTG 
AACACAGTGG TGGCCAATGC CTCCTTCACC TACCTCACCG CCCTGACGGA CTCCAACATC 
GTAGCCATCT GGGGGAGGAT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_690852.1
CDS	98..2041
Translation	MGPLSARLLMQRGRPKSDRLGKIRSLDLSGLELLSEHLDPKLLCRLTQLQELDLSNNHLETLPDNLGLSHLRVLRCANNQLGDVTALCQFPKLEELSLEGNPFLTVNDNLKVSFLLPTLRKVNGKDASSTYSQVENLNRELTSRVTAHWEKFMATLGPEEEAEKAQADFVKSAVRDVRYGPESLSEFTQWRVRMISEELVAASRTQVQKANSPEKPPEAGAAHKPRARLAALKRPDDVPLSLSPSKRACASPSAQVEGSPVAGSDGSQPAVKLEPLHFLQCHSKNNSPQDLETQLWACAFEPAWEEGATSQTVATCGGEAVCVIDCQTGIVLHKYKAPGEEFFSVAWTALMVVTQAGHKKRWSVLAAAGLRGLVRLLHVRAGFCCGVIRAHKKAIATLCFSPAHETHLFTASYDKRIILWDIGVPNQDYEFQASQLLTLDTTSIPLRLCPVASCPDARLLAGCEGGCCCWDVRLDQPQKRRVCEVEFVFSEGSEASGRRVDGLAFVNEDIVASKGSGLGTICLWSWRQTWGGRGSQSTVAVVVLARLQWSSTELAYFSLSACPDKGIVLCGDEEGNVWLYDVSNILKQPPLLPAALQAPTQILKWPQPWALGQVVTKTMVNTVVANASFTYLTALTDSNIVAIWGRM

Target ORF information:

RefSeq Version	NM_152892.3
Organism	Homo sapiens(human)
Definition	Homo sapiens leucine rich repeats and WD repeat domain containing 1 (LRWD1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_152892.3

ORF Insert Sequence:

ATGGGCCCCC TCTCGGCGCG GCTGCTAATG CAGCGCGGGC GCCCCAAGAG CGACCGGCTG 
GGGAAGATCC GGAGTCTGGA CCTGTCAGGA TTGGAGCTGC TTTCCGAGCA CCTGGACCCC 
AAACTCCTGT GCCGCCTGAC GCAGCTGCAG GAGCTTGACC TGTCTAACAA CCACCTGGAG 
ACGCTGCCGG ACAACCTGGG CCTGTCCCAC CTGCGTGTCC TCCGCTGCGC CAACAACCAG 
CTGGGGGATG TTACTGCCTT GTGCCAGTTC CCCAAGCTCG AGGAACTCAG CCTGGAGGGC 
AACCCCTTCC TGACGGTCAA TGACAACCTG AAAGTCTCCT TTCTCCTGCC CACGCTCCGT 
AAGGTCAATG GCAAGGATGC GTCCTCAACT TACTCTCAGG TGGAGAACCT GAATCGGGAG 
CTGACCAGCA GGGTCACAGC TCACTGGGAG AAGTTCATGG CCACACTGGG TCCTGAAGAG 
GAGGCTGAGA AGGCCCAGGC GGACTTTGTG AAGTCGGCTG TCAGGGATGT CCGCTACGGG 
CCCGAGTCCC TCAGCGAGTT CACCCAGTGG CGGGTGCGGA TGATCTCTGA GGAGCTGGTG 
GCCGCCAGTA GGACCCAGGT GCAAAAGGCT AACAGCCCAG AGAAGCCCCC AGAAGCTGGA 
GCTGCCCACA AGCCCAGGGC CAGACTGGCG GCCTTGAAAC GGCCAGACGA CGTCCCACTC 
AGCCTCTCTC CCAGCAAGCG GGCGTGTGCC TCCCCGTCGG CCCAGGTGGA GGGCAGCCCT 
GTGGCAGGCT CCGATGGCAG CCAGCCTGCT GTGAAGCTGG AGCCCCTGCA CTTCCTGCAG 
TGCCACAGCA AGAACAACAG CCCCCAGGAC CTCGAGACCC AGCTGTGGGC CTGTGCCTTC 
GAGCCGGCCT GGGAGGAGGG GGCCACATCC CAGACCGTGG CCACGTGCGG CGGGGAGGCT 
GTGTGCGTAA TTGATTGCCA GACGGGCATC GTGCTCCACA AGTACAAGGC ACCCGGCGAG 
GAGTTCTTTT CTGTGGCCTG GACCGCTCTG ATGGTGGTCA CACAGGCTGG CCACAAGAAG 
CGCTGGAGTG TGCTGGCGGC TGCAGGCCTA CGGGGCCTGG TCCGGCTGCT GCACGTGCGT 
GCCGGCTTCT GCTGCGGGGT CATCCGAGCC CACAAGAAGG CCATCGCCAC CCTGTGCTTC 
AGCCCCGCCC ACGAGACCCA TCTCTTCACG GCCTCCTATG ACAAGCGGAT CATCCTCTGG 
GACATCGGGG TGCCCAACCA GGACTACGAA TTCCAGGCCA GCCAGCTGCT CACACTGGAC 
ACCACCTCTA TCCCCCTGCG CCTCTGCCCT GTCGCCTCCT GCCCGGACGC CCGCCTGCTG 
GCCGGCTGCG AGGGCGGCTG CTGCTGCTGG GACGTGCGGC TGGACCAGCC CCAAAAGAGG 
AGGGTGTGTG AAGTGGAATT CGTCTTCTCT GAGGGCTCCG AGGCATCTGG ACGGAGAGTG 
GATGGGCTGG CATTTGTGAA TGAGGACATC GTGGCCTCCA AGGGGAGCGG CCTGGGCACC 
ATCTGCCTGT GGAGCTGGAG GCAGACGTGG GGGGGCCGGG GCAGCCAGTC CACGGTGGCA 
GTGGTGGTCC TGGCGCGGCT GCAATGGTCG TCCACCGAGT TGGCCTACTT CTCGCTCAGC 
GCCTGCCCTG ATAAGGGGAT TGTGCTCTGT GGGGATGAGG AGGGCAACGT GTGGCTCTAC 
GACGTCAGCA ACATCCTGAA GCAGCCACCC CTGCTGCCGG CAGCCCTGCA GGCCCCCACA 
CAGATCCTGA AGTGGCCCCA GCCCTGGGCC CTTGGCCAGG TGGTGACCAA GACCATGGTG 
AACACAGTGG TGGCCAATGC CTCCTTCACC TACCTCACCG CCCTGACGGA CTCCAACATC 
GTAGCCATCT GGGGGAGGAT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LRWD1 ( NM_152892.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_690852.1 Homo sapiens leucine rich repeats and WD repeat domain containing 1 (LRWD1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu30993
Clone ID Related Accession (Same CDS sequence)	NM_152892.3 , NM_152892.2 , NM_152892.1
Accession Version	NM_152892.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1944bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-02
Organism	Homo sapiens(human)
Product	leucine-rich repeat and WD repeat-containing protein 1 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from HY037356.1, BC030547.1 and BC009436.1. On Dec 4, 2015 this sequence version replaced NM_152892.1. Transcript Variant: This variant (1) encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AL133057.1, BC030547.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGCCCCC TCTCGGCGCG GCTGCTAATG CAGCGCGGGC GCCCCAAGAG CGACCGGCTG 
GGGAAGATCC GGAGTCTGGA CCTGTCAGGA TTGGAGCTGC TTTCCGAGCA CCTGGACCCC 
AAACTCCTGT GCCGCCTGAC GCAGCTGCAG GAGCTTGACC TGTCTAACAA CCACCTGGAG 
ACGCTGCCGG ACAACCTGGG CCTGTCCCAC CTGCGTGTCC TCCGCTGCGC CAACAACCAG 
CTGGGGGATG TTACTGCCTT GTGCCAGTTC CCCAAGCTCG AGGAACTCAG CCTGGAGGGC 
AACCCCTTCC TGACGGTCAA TGACAACCTG AAAGTCTCCT TTCTCCTGCC CACGCTCCGT 
AAGGTCAATG GCAAGGATGC GTCCTCAACT TACTCTCAGG TGGAGAACCT GAATCGGGAG 
CTGACCAGCA GGGTCACAGC TCACTGGGAG AAGTTCATGG CCACACTGGG TCCTGAAGAG 
GAGGCTGAGA AGGCCCAGGC GGACTTTGTG AAGTCGGCTG TCAGGGATGT CCGCTACGGG 
CCCGAGTCCC TCAGCGAGTT CACCCAGTGG CGGGTGCGGA TGATCTCTGA GGAGCTGGTG 
GCCGCCAGTA GGACCCAGGT GCAAAAGGCT AACAGCCCAG AGAAGCCCCC AGAAGCTGGA 
GCTGCCCACA AGCCCAGGGC CAGACTGGCG GCCTTGAAAC GGCCAGACGA CGTCCCACTC 
AGCCTCTCTC CCAGCAAGCG GGCGTGTGCC TCCCCGTCGG CCCAGGTGGA GGGCAGCCCT 
GTGGCAGGCT CCGATGGCAG CCAGCCTGCT GTGAAGCTGG AGCCCCTGCA CTTCCTGCAG 
TGCCACAGCA AGAACAACAG CCCCCAGGAC CTCGAGACCC AGCTGTGGGC CTGTGCCTTC 
GAGCCGGCCT GGGAGGAGGG GGCCACATCC CAGACCGTGG CCACGTGCGG CGGGGAGGCT 
GTGTGCGTAA TTGATTGCCA GACGGGCATC GTGCTCCACA AGTACAAGGC ACCCGGCGAG 
GAGTTCTTTT CTGTGGCCTG GACCGCTCTG ATGGTGGTCA CACAGGCTGG CCACAAGAAG 
CGCTGGAGTG TGCTGGCGGC TGCAGGCCTA CGGGGCCTGG TCCGGCTGCT GCACGTGCGT 
GCCGGCTTCT GCTGCGGGGT CATCCGAGCC CACAAGAAGG CCATCGCCAC CCTGTGCTTC 
AGCCCCGCCC ACGAGACCCA TCTCTTCACG GCCTCCTATG ACAAGCGGAT CATCCTCTGG 
GACATCGGGG TGCCCAACCA GGACTACGAA TTCCAGGCCA GCCAGCTGCT CACACTGGAC 
ACCACCTCTA TCCCCCTGCG CCTCTGCCCT GTCGCCTCCT GCCCGGACGC CCGCCTGCTG 
GCCGGCTGCG AGGGCGGCTG CTGCTGCTGG GACGTGCGGC TGGACCAGCC CCAAAAGAGG 
AGGGTGTGTG AAGTGGAATT CGTCTTCTCT GAGGGCTCCG AGGCATCTGG ACGGAGAGTG 
GATGGGCTGG CATTTGTGAA TGAGGACATC GTGGCCTCCA AGGGGAGCGG CCTGGGCACC 
ATCTGCCTGT GGAGCTGGAG GCAGACGTGG GGGGGCCGGG GCAGCCAGTC CACGGTGGCA 
GTGGTGGTCC TGGCGCGGCT GCAATGGTCG TCCACCGAGT TGGCCTACTT CTCGCTCAGC 
GCCTGCCCTG ATAAGGGGAT TGTGCTCTGT GGGGATGAGG AGGGCAACGT GTGGCTCTAC 
GACGTCAGCA ACATCCTGAA GCAGCCACCC CTGCTGCCGG CAGCCCTGCA GGCCCCCACA 
CAGATCCTGA AGTGGCCCCA GCCCTGGGCC CTTGGCCAGG TGGTGACCAA GACCATGGTG 
AACACAGTGG TGGCCAATGC CTCCTTCACC TACCTCACCG CCCTGACGGA CTCCAACATC 
GTAGCCATCT GGGGGAGGAT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_690852.1
CDS	199..2142
Translation	MGPLSARLLMQRGRPKSDRLGKIRSLDLSGLELLSEHLDPKLLCRLTQLQELDLSNNHLETLPDNLGLSHLRVLRCANNQLGDVTALCQFPKLEELSLEGNPFLTVNDNLKVSFLLPTLRKVNGKDASSTYSQVENLNRELTSRVTAHWEKFMATLGPEEEAEKAQADFVKSAVRDVRYGPESLSEFTQWRVRMISEELVAASRTQVQKANSPEKPPEAGAAHKPRARLAALKRPDDVPLSLSPSKRACASPSAQVEGSPVAGSDGSQPAVKLEPLHFLQCHSKNNSPQDLETQLWACAFEPAWEEGATSQTVATCGGEAVCVIDCQTGIVLHKYKAPGEEFFSVAWTALMVVTQAGHKKRWSVLAAAGLRGLVRLLHVRAGFCCGVIRAHKKAIATLCFSPAHETHLFTASYDKRIILWDIGVPNQDYEFQASQLLTLDTTSIPLRLCPVASCPDARLLAGCEGGCCCWDVRLDQPQKRRVCEVEFVFSEGSEASGRRVDGLAFVNEDIVASKGSGLGTICLWSWRQTWGGRGSQSTVAVVVLARLQWSSTELAYFSLSACPDKGIVLCGDEEGNVWLYDVSNILKQPPLLPAALQAPTQILKWPQPWALGQVVTKTMVNTVVANASFTYLTALTDSNIVAIWGRM

Target ORF information:

RefSeq Version	NM_152892.2
Organism	Homo sapiens(human)
Definition	Homo sapiens leucine rich repeats and WD repeat domain containing 1 (LRWD1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_152892.2

ORF Insert Sequence:

ATGGGCCCCC TCTCGGCGCG GCTGCTAATG CAGCGCGGGC GCCCCAAGAG CGACCGGCTG 
GGGAAGATCC GGAGTCTGGA CCTGTCAGGA TTGGAGCTGC TTTCCGAGCA CCTGGACCCC 
AAACTCCTGT GCCGCCTGAC GCAGCTGCAG GAGCTTGACC TGTCTAACAA CCACCTGGAG 
ACGCTGCCGG ACAACCTGGG CCTGTCCCAC CTGCGTGTCC TCCGCTGCGC CAACAACCAG 
CTGGGGGATG TTACTGCCTT GTGCCAGTTC CCCAAGCTCG AGGAACTCAG CCTGGAGGGC 
AACCCCTTCC TGACGGTCAA TGACAACCTG AAAGTCTCCT TTCTCCTGCC CACGCTCCGT 
AAGGTCAATG GCAAGGATGC GTCCTCAACT TACTCTCAGG TGGAGAACCT GAATCGGGAG 
CTGACCAGCA GGGTCACAGC TCACTGGGAG AAGTTCATGG CCACACTGGG TCCTGAAGAG 
GAGGCTGAGA AGGCCCAGGC GGACTTTGTG AAGTCGGCTG TCAGGGATGT CCGCTACGGG 
CCCGAGTCCC TCAGCGAGTT CACCCAGTGG CGGGTGCGGA TGATCTCTGA GGAGCTGGTG 
GCCGCCAGTA GGACCCAGGT GCAAAAGGCT AACAGCCCAG AGAAGCCCCC AGAAGCTGGA 
GCTGCCCACA AGCCCAGGGC CAGACTGGCG GCCTTGAAAC GGCCAGACGA CGTCCCACTC 
AGCCTCTCTC CCAGCAAGCG GGCGTGTGCC TCCCCGTCGG CCCAGGTGGA GGGCAGCCCT 
GTGGCAGGCT CCGATGGCAG CCAGCCTGCT GTGAAGCTGG AGCCCCTGCA CTTCCTGCAG 
TGCCACAGCA AGAACAACAG CCCCCAGGAC CTCGAGACCC AGCTGTGGGC CTGTGCCTTC 
GAGCCGGCCT GGGAGGAGGG GGCCACATCC CAGACCGTGG CCACGTGCGG CGGGGAGGCT 
GTGTGCGTAA TTGATTGCCA GACGGGCATC GTGCTCCACA AGTACAAGGC ACCCGGCGAG 
GAGTTCTTTT CTGTGGCCTG GACCGCTCTG ATGGTGGTCA CACAGGCTGG CCACAAGAAG 
CGCTGGAGTG TGCTGGCGGC TGCAGGCCTA CGGGGCCTGG TCCGGCTGCT GCACGTGCGT 
GCCGGCTTCT GCTGCGGGGT CATCCGAGCC CACAAGAAGG CCATCGCCAC CCTGTGCTTC 
AGCCCCGCCC ACGAGACCCA TCTCTTCACG GCCTCCTATG ACAAGCGGAT CATCCTCTGG 
GACATCGGGG TGCCCAACCA GGACTACGAA TTCCAGGCCA GCCAGCTGCT CACACTGGAC 
ACCACCTCTA TCCCCCTGCG CCTCTGCCCT GTCGCCTCCT GCCCGGACGC CCGCCTGCTG 
GCCGGCTGCG AGGGCGGCTG CTGCTGCTGG GACGTGCGGC TGGACCAGCC CCAAAAGAGG 
AGGGTGTGTG AAGTGGAATT CGTCTTCTCT GAGGGCTCCG AGGCATCTGG ACGGAGAGTG 
GATGGGCTGG CATTTGTGAA TGAGGACATC GTGGCCTCCA AGGGGAGCGG CCTGGGCACC 
ATCTGCCTGT GGAGCTGGAG GCAGACGTGG GGGGGCCGGG GCAGCCAGTC CACGGTGGCA 
GTGGTGGTCC TGGCGCGGCT GCAATGGTCG TCCACCGAGT TGGCCTACTT CTCGCTCAGC 
GCCTGCCCTG ATAAGGGGAT TGTGCTCTGT GGGGATGAGG AGGGCAACGT GTGGCTCTAC 
GACGTCAGCA ACATCCTGAA GCAGCCACCC CTGCTGCCGG CAGCCCTGCA GGCCCCCACA 
CAGATCCTGA AGTGGCCCCA GCCCTGGGCC CTTGGCCAGG TGGTGACCAA GACCATGGTG 
AACACAGTGG TGGCCAATGC CTCCTTCACC TACCTCACCG CCCTGACGGA CTCCAACATC 
GTAGCCATCT GGGGGAGGAT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LRWD1 ( NM_152892.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_690852.1 Homo sapiens leucine-rich repeats and WD repeat domain containing 1 (LRWD1), mRNA.

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CloneID	OHu30993
Clone ID Related Accession (Same CDS sequence)	NM_152892.3 , NM_152892.2 , NM_152892.1
Accession Version	NM_152892.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1944bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-14
Organism	Homo sapiens(Human)
Product	leucine-rich repeat and WD repeat-containing protein 1
Comment

ATGGGCCCCC TCTCGGCGCG GCTGCTAATG CAGCGCGGGC GCCCCAAGAG CGACCGGCTG 
GGGAAGATCC GGAGTCTGGA CCTGTCAGGA TTGGAGCTGC TTTCCGAGCA CCTGGACCCC 
AAACTCCTGT GCCGCCTGAC GCAGCTGCAG GAGCTTGACC TGTCTAACAA CCACCTGGAG 
ACGCTGCCGG ACAACCTGGG CCTGTCCCAC CTGCGTGTCC TCCGCTGCGC CAACAACCAG 
CTGGGGGATG TTACTGCCTT GTGCCAGTTC CCCAAGCTCG AGGAACTCAG CCTGGAGGGC 
AACCCCTTCC TGACGGTCAA TGACAACCTG AAAGTCTCCT TTCTCCTGCC CACGCTCCGT 
AAGGTCAATG GCAAGGATGC GTCCTCAACT TACTCTCAGG TGGAGAACCT GAATCGGGAG 
CTGACCAGCA GGGTCACAGC TCACTGGGAG AAGTTCATGG CCACACTGGG TCCTGAAGAG 
GAGGCTGAGA AGGCCCAGGC GGACTTTGTG AAGTCGGCTG TCAGGGATGT CCGCTACGGG 
CCCGAGTCCC TCAGCGAGTT CACCCAGTGG CGGGTGCGGA TGATCTCTGA GGAGCTGGTG 
GCCGCCAGTA GGACCCAGGT GCAAAAGGCT AACAGCCCAG AGAAGCCCCC AGAAGCTGGA 
GCTGCCCACA AGCCCAGGGC CAGACTGGCG GCCTTGAAAC GGCCAGACGA CGTCCCACTC 
AGCCTCTCTC CCAGCAAGCG GGCGTGTGCC TCCCCGTCGG CCCAGGTGGA GGGCAGCCCT 
GTGGCAGGCT CCGATGGCAG CCAGCCTGCT GTGAAGCTGG AGCCCCTGCA CTTCCTGCAG 
TGCCACAGCA AGAACAACAG CCCCCAGGAC CTCGAGACCC AGCTGTGGGC CTGTGCCTTC 
GAGCCGGCCT GGGAGGAGGG GGCCACATCC CAGACCGTGG CCACGTGCGG CGGGGAGGCT 
GTGTGCGTAA TTGATTGCCA GACGGGCATC GTGCTCCACA AGTACAAGGC ACCCGGCGAG 
GAGTTCTTTT CTGTGGCCTG GACCGCTCTG ATGGTGGTCA CACAGGCTGG CCACAAGAAG 
CGCTGGAGTG TGCTGGCGGC TGCAGGCCTA CGGGGCCTGG TCCGGCTGCT GCACGTGCGT 
GCCGGCTTCT GCTGCGGGGT CATCCGAGCC CACAAGAAGG CCATCGCCAC CCTGTGCTTC 
AGCCCCGCCC ACGAGACCCA TCTCTTCACG GCCTCCTATG ACAAGCGGAT CATCCTCTGG 
GACATCGGGG TGCCCAACCA GGACTACGAA TTCCAGGCCA GCCAGCTGCT CACACTGGAC 
ACCACCTCTA TCCCCCTGCG CCTCTGCCCT GTCGCCTCCT GCCCGGACGC CCGCCTGCTG 
GCCGGCTGCG AGGGCGGCTG CTGCTGCTGG GACGTGCGGC TGGACCAGCC CCAAAAGAGG 
AGGGTGTGTG AAGTGGAATT CGTCTTCTCT GAGGGCTCCG AGGCATCTGG ACGGAGAGTG 
GATGGGCTGG CATTTGTGAA TGAGGACATC GTGGCCTCCA AGGGGAGCGG CCTGGGCACC 
ATCTGCCTGT GGAGCTGGAG GCAGACGTGG GGGGGCCGGG GCAGCCAGTC CACGGTGGCA 
GTGGTGGTCC TGGCGCGGCT GCAATGGTCG TCCACCGAGT TGGCCTACTT CTCGCTCAGC 
GCCTGCCCTG ATAAGGGGAT TGTGCTCTGT GGGGATGAGG AGGGCAACGT GTGGCTCTAC 
GACGTCAGCA ACATCCTGAA GCAGCCACCC CTGCTGCCGG CAGCCCTGCA GGCCCCCACA 
CAGATCCTGA AGTGGCCCCA GCCCTGGGCC CTTGGCCAGG TGGTGACCAA GACCATGGTG 
AACACAGTGG TGGCCAATGC CTCCTTCACC TACCTCACCG CCCTGACGGA CTCCAACATC 
GTAGCCATCT GGGGGAGGAT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

NP_690852.1

Translation

MGPLSARLLMQRGRPKSDRLGKIRSLDLSGLELLSEHLDPKLLCRLTQLQELDLSNNHLETLPDNLGLSHLRVLRCANNQLGDVTALCQFPKLEELSLEGNPFLTVNDNLKVSFLLPTLRKVNGKDASSTYSQVENLNRELTSRVTAHWEKFMATLGPEEEAEKAQADFVKSAVRDVRYGPESLSEFTQWRVRMISEELVAASRTQVQKANSPEKPPEAGAAHKPRARLAALKRPDDVPLSLSPSKRACASPSAQVEGSPVAGSDGSQPAVKLEPLHFLQCHSKNNSPQDLETQLWACAFEPAWEEGATSQTVATCGGEAVCVIDCQTGIVLHKYKAPGEEFFSVAWTALMVVTQAGHKKRWSVLAAAGLRGLVRLLHVRAGFCCGVIRAHKKAIATLCFSPAHETHLFTASYDKRIILWDIGVPNQDYEFQASQLLTLDTTSIPLRLCPVASCPDARLLAGCEGGCCCWDVRLDQPQKRRVCEVEFVFSEGSEASGRRVDGLAFVNEDIVASKGSGLGTICLWSWRQTWGGRGSQSTVAVVVLARLQWSSTELAYFSLSACPDKGIVLCGDEEGNVWLYDVSNILKQPPLLPAALQAPTQILKWPQPWALGQVVTKTMVNTVVANASFTYLTALTDSNIVAIWGRM

Target ORF information:

RefSeq Version	NM_152892.1
Organism	Homo sapiens(Human)
Definition	Homo sapiens leucine-rich repeats and WD repeat domain containing 1 (LRWD1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_152892.1

ORF Insert Sequence:

ATGGGCCCCC TCTCGGCGCG GCTGCTAATG CAGCGCGGGC GCCCCAAGAG CGACCGGCTG 
GGGAAGATCC GGAGTCTGGA CCTGTCAGGA TTGGAGCTGC TTTCCGAGCA CCTGGACCCC 
AAACTCCTGT GCCGCCTGAC GCAGCTGCAG GAGCTTGACC TGTCTAACAA CCACCTGGAG 
ACGCTGCCGG ACAACCTGGG CCTGTCCCAC CTGCGTGTCC TCCGCTGCGC CAACAACCAG 
CTGGGGGATG TTACTGCCTT GTGCCAGTTC CCCAAGCTCG AGGAACTCAG CCTGGAGGGC 
AACCCCTTCC TGACGGTCAA TGACAACCTG AAAGTCTCCT TTCTCCTGCC CACGCTCCGT 
AAGGTCAATG GCAAGGATGC GTCCTCAACT TACTCTCAGG TGGAGAACCT GAATCGGGAG 
CTGACCAGCA GGGTCACAGC TCACTGGGAG AAGTTCATGG CCACACTGGG TCCTGAAGAG 
GAGGCTGAGA AGGCCCAGGC GGACTTTGTG AAGTCGGCTG TCAGGGATGT CCGCTACGGG 
CCCGAGTCCC TCAGCGAGTT CACCCAGTGG CGGGTGCGGA TGATCTCTGA GGAGCTGGTG 
GCCGCCAGTA GGACCCAGGT GCAAAAGGCT AACAGCCCAG AGAAGCCCCC AGAAGCTGGA 
GCTGCCCACA AGCCCAGGGC CAGACTGGCG GCCTTGAAAC GGCCAGACGA CGTCCCACTC 
AGCCTCTCTC CCAGCAAGCG GGCGTGTGCC TCCCCGTCGG CCCAGGTGGA GGGCAGCCCT 
GTGGCAGGCT CCGATGGCAG CCAGCCTGCT GTGAAGCTGG AGCCCCTGCA CTTCCTGCAG 
TGCCACAGCA AGAACAACAG CCCCCAGGAC CTCGAGACCC AGCTGTGGGC CTGTGCCTTC 
GAGCCGGCCT GGGAGGAGGG GGCCACATCC CAGACCGTGG CCACGTGCGG CGGGGAGGCT 
GTGTGCGTAA TTGATTGCCA GACGGGCATC GTGCTCCACA AGTACAAGGC ACCCGGCGAG 
GAGTTCTTTT CTGTGGCCTG GACCGCTCTG ATGGTGGTCA CACAGGCTGG CCACAAGAAG 
CGCTGGAGTG TGCTGGCGGC TGCAGGCCTA CGGGGCCTGG TCCGGCTGCT GCACGTGCGT 
GCCGGCTTCT GCTGCGGGGT CATCCGAGCC CACAAGAAGG CCATCGCCAC CCTGTGCTTC 
AGCCCCGCCC ACGAGACCCA TCTCTTCACG GCCTCCTATG ACAAGCGGAT CATCCTCTGG 
GACATCGGGG TGCCCAACCA GGACTACGAA TTCCAGGCCA GCCAGCTGCT CACACTGGAC 
ACCACCTCTA TCCCCCTGCG CCTCTGCCCT GTCGCCTCCT GCCCGGACGC CCGCCTGCTG 
GCCGGCTGCG AGGGCGGCTG CTGCTGCTGG GACGTGCGGC TGGACCAGCC CCAAAAGAGG 
AGGGTGTGTG AAGTGGAATT CGTCTTCTCT GAGGGCTCCG AGGCATCTGG ACGGAGAGTG 
GATGGGCTGG CATTTGTGAA TGAGGACATC GTGGCCTCCA AGGGGAGCGG CCTGGGCACC 
ATCTGCCTGT GGAGCTGGAG GCAGACGTGG GGGGGCCGGG GCAGCCAGTC CACGGTGGCA 
GTGGTGGTCC TGGCGCGGCT GCAATGGTCG TCCACCGAGT TGGCCTACTT CTCGCTCAGC 
GCCTGCCCTG ATAAGGGGAT TGTGCTCTGT GGGGATGAGG AGGGCAACGT GTGGCTCTAC 
GACGTCAGCA ACATCCTGAA GCAGCCACCC CTGCTGCCGG CAGCCCTGCA GGCCCCCACA 
CAGATCCTGA AGTGGCCCCA GCCCTGGGCC CTTGGCCAGG TGGTGACCAA GACCATGGTG 
AACACAGTGG TGGCCAATGC CTCCTTCACC TACCTCACCG CCCTGACGGA CTCCAACATC 
GTAGCCATCT GGGGGAGGAT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LRWD1 ( NM_001317721.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001304650.1 Homo sapiens leucine rich repeats and WD repeat domain containing 1 (LRWD1), transcript variant 2, mRNA.

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CloneID	OHu41426
Clone ID Related Accession (Same CDS sequence)	NM_001317721.1
Accession Version	NM_001317721.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1488bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-03
Organism	Homo sapiens(human)
Product	leucine-rich repeat and WD repeat-containing protein 1 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from HY037356.1, HY051509.1, BC030547.1 and BC009436.1. On Dec 4, 2015 this sequence version replaced XM_005250204.1. Transcript Variant: This variant (2) uses an alternate splice junction at the 3' end of the first exon compared to variant 1. The resulting isoform (2) is shorter at the N-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163658.235375.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCACAC TGGGTCCTGA AGAGGAGGCT GAGAAGGCCC AGGCGGACTT TGTGAAGTCG 
GCTGTCAGGG ATGTCCGCTA CGGGCCCGAG TCCCTCAGCG AGTTCACCCA GTGGCGGGTG 
CGGATGATCT CTGAGGAGCT GGTGGCCGCC AGTAGGACCC AGGTGCAAAA GGCTAACAGC 
CCAGAGAAGC CCCCAGAAGC TGGAGCTGCC CACAAGCCCA GGGCCAGACT GGCGGCCTTG 
AAACGGCCAG ACGACGTCCC ACTCAGCCTC TCTCCCAGCA AGCGGGCGTG TGCCTCCCCG 
TCGGCCCAGG TGGAGGGCAG CCCTGTGGCA GGCTCCGATG GCAGCCAGCC TGCTGTGAAG 
CTGGAGCCCC TGCACTTCCT GCAGTGCCAC AGCAAGAACA ACAGCCCCCA GGACCTCGAG 
ACCCAGCTGT GGGCCTGTGC CTTCGAGCCG GCCTGGGAGG AGGGGGCCAC ATCCCAGACC 
GTGGCCACGT GCGGCGGGGA GGCTGTGTGC GTAATTGATT GCCAGACGGG CATCGTGCTC 
CACAAGTACA AGGCACCCGG CGAGGAGTTC TTTTCTGTGG CCTGGACCGC TCTGATGGTG 
GTCACACAGG CTGGCCACAA GAAGCGCTGG AGTGTGCTGG CGGCTGCAGG CCTACGGGGC 
CTGGTCCGGC TGCTGCACGT GCGTGCCGGC TTCTGCTGCG GGGTCATCCG AGCCCACAAG 
AAGGCCATCG CCACCCTGTG CTTCAGCCCC GCCCACGAGA CCCATCTCTT CACGGCCTCC 
TATGACAAGC GGATCATCCT CTGGGACATC GGGGTGCCCA ACCAGGACTA CGAATTCCAG 
GCCAGCCAGC TGCTCACACT GGACACCACC TCTATCCCCC TGCGCCTCTG CCCTGTCGCC 
TCCTGCCCGG ACGCCCGCCT GCTGGCCGGC TGCGAGGGCG GCTGCTGCTG CTGGGACGTG 
CGGCTGGACC AGCCCCAAAA GAGGAGGGTG TGTGAAGTGG AATTCGTCTT CTCTGAGGGC 
TCCGAGGCAT CTGGACGGAG AGTGGATGGG CTGGCATTTG TGAATGAGGA CATCGTGGCC 
TCCAAGGGGA GCGGCCTGGG CACCATCTGC CTGTGGAGCT GGAGGCAGAC GTGGGGGGGC 
CGGGGCAGCC AGTCCACGGT GGCAGTGGTG GTCCTGGCGC GGCTGCAATG GTCGTCCACC 
GAGTTGGCCT ACTTCTCGCT CAGCGCCTGC CCTGATAAGG GGATTGTGCT CTGTGGGGAT 
GAGGAGGGCA ACGTGTGGCT CTACGACGTC AGCAACATCC TGAAGCAGCC ACCCCTGCTG 
CCGGCAGCCC TGCAGGCCCC CACACAGATC CTGAAGTGGC CCCAGCCCTG GGCCCTTGGC 
CAGGTGGTGA CCAAGACCAT GGTGAACACA GTGGTGGCCA ATGCCTCCTT CACCTACCTC 
ACCGCCCTGA CGGACTCCAA CATCGTAGCC ATCTGGGGGA GGATGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001304650.1
CDS	673..2160
Translation	MATLGPEEEAEKAQADFVKSAVRDVRYGPESLSEFTQWRVRMISEELVAASRTQVQKANSPEKPPEAGAAHKPRARLAALKRPDDVPLSLSPSKRACASPSAQVEGSPVAGSDGSQPAVKLEPLHFLQCHSKNNSPQDLETQLWACAFEPAWEEGATSQTVATCGGEAVCVIDCQTGIVLHKYKAPGEEFFSVAWTALMVVTQAGHKKRWSVLAAAGLRGLVRLLHVRAGFCCGVIRAHKKAIATLCFSPAHETHLFTASYDKRIILWDIGVPNQDYEFQASQLLTLDTTSIPLRLCPVASCPDARLLAGCEGGCCCWDVRLDQPQKRRVCEVEFVFSEGSEASGRRVDGLAFVNEDIVASKGSGLGTICLWSWRQTWGGRGSQSTVAVVVLARLQWSSTELAYFSLSACPDKGIVLCGDEEGNVWLYDVSNILKQPPLLPAALQAPTQILKWPQPWALGQVVTKTMVNTVVANASFTYLTALTDSNIVAIWGRM

Target ORF information:

RefSeq Version	NM_001317721.1
Organism	Homo sapiens(human)
Definition	Homo sapiens leucine rich repeats and WD repeat domain containing 1 (LRWD1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001317721.1

ORF Insert Sequence:

ATGGCCACAC TGGGTCCTGA AGAGGAGGCT GAGAAGGCCC AGGCGGACTT TGTGAAGTCG 
GCTGTCAGGG ATGTCCGCTA CGGGCCCGAG TCCCTCAGCG AGTTCACCCA GTGGCGGGTG 
CGGATGATCT CTGAGGAGCT GGTGGCCGCC AGTAGGACCC AGGTGCAAAA GGCTAACAGC 
CCAGAGAAGC CCCCAGAAGC TGGAGCTGCC CACAAGCCCA GGGCCAGACT GGCGGCCTTG 
AAACGGCCAG ACGACGTCCC ACTCAGCCTC TCTCCCAGCA AGCGGGCGTG TGCCTCCCCG 
TCGGCCCAGG TGGAGGGCAG CCCTGTGGCA GGCTCCGATG GCAGCCAGCC TGCTGTGAAG 
CTGGAGCCCC TGCACTTCCT GCAGTGCCAC AGCAAGAACA ACAGCCCCCA GGACCTCGAG 
ACCCAGCTGT GGGCCTGTGC CTTCGAGCCG GCCTGGGAGG AGGGGGCCAC ATCCCAGACC 
GTGGCCACGT GCGGCGGGGA GGCTGTGTGC GTAATTGATT GCCAGACGGG CATCGTGCTC 
CACAAGTACA AGGCACCCGG CGAGGAGTTC TTTTCTGTGG CCTGGACCGC TCTGATGGTG 
GTCACACAGG CTGGCCACAA GAAGCGCTGG AGTGTGCTGG CGGCTGCAGG CCTACGGGGC 
CTGGTCCGGC TGCTGCACGT GCGTGCCGGC TTCTGCTGCG GGGTCATCCG AGCCCACAAG 
AAGGCCATCG CCACCCTGTG CTTCAGCCCC GCCCACGAGA CCCATCTCTT CACGGCCTCC 
TATGACAAGC GGATCATCCT CTGGGACATC GGGGTGCCCA ACCAGGACTA CGAATTCCAG 
GCCAGCCAGC TGCTCACACT GGACACCACC TCTATCCCCC TGCGCCTCTG CCCTGTCGCC 
TCCTGCCCGG ACGCCCGCCT GCTGGCCGGC TGCGAGGGCG GCTGCTGCTG CTGGGACGTG 
CGGCTGGACC AGCCCCAAAA GAGGAGGGTG TGTGAAGTGG AATTCGTCTT CTCTGAGGGC 
TCCGAGGCAT CTGGACGGAG AGTGGATGGG CTGGCATTTG TGAATGAGGA CATCGTGGCC 
TCCAAGGGGA GCGGCCTGGG CACCATCTGC CTGTGGAGCT GGAGGCAGAC GTGGGGGGGC 
CGGGGCAGCC AGTCCACGGT GGCAGTGGTG GTCCTGGCGC GGCTGCAATG GTCGTCCACC 
GAGTTGGCCT ACTTCTCGCT CAGCGCCTGC CCTGATAAGG GGATTGTGCT CTGTGGGGAT 
GAGGAGGGCA ACGTGTGGCT CTACGACGTC AGCAACATCC TGAAGCAGCC ACCCCTGCTG 
CCGGCAGCCC TGCAGGCCCC CACACAGATC CTGAAGTGGC CCCAGCCCTG GGCCCTTGGC 
CAGGTGGTGA CCAAGACCAT GGTGAACACA GTGGTGGCCA ATGCCTCCTT CACCTACCTC 
ACCGCCCTGA CGGACTCCAA CATCGTAGCC ATCTGGGGGA GGATGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LRWD1 ( XM_017011845.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016867334.1 Homo sapiens leucine-rich repeats and WD repeat domain containing 1 (LRWD1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu97093
Clone ID Related Accession (Same CDS sequence)	XM_017011845.1
Accession Version	XM_017011845.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1365bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	leucine-rich repeat and WD repeat-containing protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCTCTG AGGAGCTGGT GGCCGCCAGT AGGACCCAGG TGCAAAAGGC TAACAGCCCA 
GAGAAGCCCC CAGAAGCTGG AGCTGCCCAC AAGCCCAGGG CCAGACTGGC GGCCTTGAAA 
CGGCCAGACG ACGTCCCACT CAGCCTCTCT CCCAGCAAGC GGGCGTGTGC CTCCCCGTCG 
GCCCAGGTGG AGGGCAGCCC TGTGGCAGGC TCCGATGGCA GCCAGCCTGC TGTGAAGCTG 
GAGCCCCTGC ACTTCCTGCA GTGCCACAGC AAGAACAACA GCCCCCAGGA CCTCGAGACC 
CAGCTGTGGG CCTGTGCCTT CGAGCCGGCC TGGGAGGAGG GGGCCACATC CCAGACCGTG 
GCCACGTGCG GCGGGGAGGC TGTGTGCGTA ATTGATTGCC AGACGGGCAT CGTGCTCCAC 
AAGTACAAGG CACCCGGCGA GGAGTTCTTT TCTGTGGCCT GGACCGCTCT GATGGTGGTC 
ACACAGGCTG GCCACAAGAA GCGCTGGAGT GTGCTGGCGG CTGCAGGCCT ACGGGGCCTG 
GTCCGGCTGC TGCACGTGCG TGCCGGCTTC TGCTGCGGGG TCATCCGAGC CCACAAGAAG 
GCCATCGCCA CCCTGTGCTT CAGCCCCGCC CACGAGACCC ATCTCTTCAC GGCCTCCTAT 
GACAAGCGGA TCATCCTCTG GGACATCGGG GTGCCCAACC AGGACTACGA ATTCCAGGCC 
AGCCAGCTGC TCACACTGGA CACCACCTCT ATCCCCCTGC GCCTCTGCCC TGTCGCCTCC 
TGCCCGGACG CCCGCCTGCT GGCCGGCTGC GAGGGCGGCT GCTGCTGCTG GGACGTGCGG 
CTGGACCAGC CCCAAAAGAG GAGGGTGTGT GAAGTGGAAT TCGTCTTCTC TGAGGGCTCC 
GAGGCATCTG GACGGAGAGT GGATGGGCTG GCATTTGTGA ATGAGGACAT CGTGGCCTCC 
AAGGGGAGCG GCCTGGGCAC CATCTGCCTG TGGAGCTGGA GGCAGACGTG GGGGGGCCGG 
GGCAGCCAGT CCACGGTGGC AGTGGTGGTC CTGGCGCGGC TGCAATGGTC GTCCACCGAG 
TTGGCCTACT TCTCGCTCAG CGCCTGCCCT GATAAGGGGA TTGTGCTCTG TGGGGATGAG 
GAGGGCAACG TGTGGCTCTA CGACGTCAGC AACATCCTGA AGCAGCCACC CCTGCTGCCG 
GCAGCCCTGC AGGCCCCCAC ACAGATCCTG AAGTGGCCCC AGCCCTGGGC CCTTGGCCAG 
GTGGTGACCA AGACCATGGT GAACACAGTG GTGGCCAATG CCTCCTTCAC CTACCTCACC 
GCCCTGACGG ACTCCAACAT CGTAGCCATC TGGGGGAGGA TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016867334.1
CDS	733..2097
Translation	MISEELVAASRTQVQKANSPEKPPEAGAAHKPRARLAALKRPDDVPLSLSPSKRACASPSAQVEGSPVAGSDGSQPAVKLEPLHFLQCHSKNNSPQDLETQLWACAFEPAWEEGATSQTVATCGGEAVCVIDCQTGIVLHKYKAPGEEFFSVAWTALMVVTQAGHKKRWSVLAAAGLRGLVRLLHVRAGFCCGVIRAHKKAIATLCFSPAHETHLFTASYDKRIILWDIGVPNQDYEFQASQLLTLDTTSIPLRLCPVASCPDARLLAGCEGGCCCWDVRLDQPQKRRVCEVEFVFSEGSEASGRRVDGLAFVNEDIVASKGSGLGTICLWSWRQTWGGRGSQSTVAVVVLARLQWSSTELAYFSLSACPDKGIVLCGDEEGNVWLYDVSNILKQPPLLPAALQAPTQILKWPQPWALGQVVTKTMVNTVVANASFTYLTALTDSNIVAIWGRM

Target ORF information:

RefSeq Version	XM_017011845.1
Organism	Homo sapiens(human)
Definition	Homo sapiens leucine-rich repeats and WD repeat domain containing 1 (LRWD1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017011845.1

ORF Insert Sequence:

ATGATCTCTG AGGAGCTGGT GGCCGCCAGT AGGACCCAGG TGCAAAAGGC TAACAGCCCA 
GAGAAGCCCC CAGAAGCTGG AGCTGCCCAC AAGCCCAGGG CCAGACTGGC GGCCTTGAAA 
CGGCCAGACG ACGTCCCACT CAGCCTCTCT CCCAGCAAGC GGGCGTGTGC CTCCCCGTCG 
GCCCAGGTGG AGGGCAGCCC TGTGGCAGGC TCCGATGGCA GCCAGCCTGC TGTGAAGCTG 
GAGCCCCTGC ACTTCCTGCA GTGCCACAGC AAGAACAACA GCCCCCAGGA CCTCGAGACC 
CAGCTGTGGG CCTGTGCCTT CGAGCCGGCC TGGGAGGAGG GGGCCACATC CCAGACCGTG 
GCCACGTGCG GCGGGGAGGC TGTGTGCGTA ATTGATTGCC AGACGGGCAT CGTGCTCCAC 
AAGTACAAGG CACCCGGCGA GGAGTTCTTT TCTGTGGCCT GGACCGCTCT GATGGTGGTC 
ACACAGGCTG GCCACAAGAA GCGCTGGAGT GTGCTGGCGG CTGCAGGCCT ACGGGGCCTG 
GTCCGGCTGC TGCACGTGCG TGCCGGCTTC TGCTGCGGGG TCATCCGAGC CCACAAGAAG 
GCCATCGCCA CCCTGTGCTT CAGCCCCGCC CACGAGACCC ATCTCTTCAC GGCCTCCTAT 
GACAAGCGGA TCATCCTCTG GGACATCGGG GTGCCCAACC AGGACTACGA ATTCCAGGCC 
AGCCAGCTGC TCACACTGGA CACCACCTCT ATCCCCCTGC GCCTCTGCCC TGTCGCCTCC 
TGCCCGGACG CCCGCCTGCT GGCCGGCTGC GAGGGCGGCT GCTGCTGCTG GGACGTGCGG 
CTGGACCAGC CCCAAAAGAG GAGGGTGTGT GAAGTGGAAT TCGTCTTCTC TGAGGGCTCC 
GAGGCATCTG GACGGAGAGT GGATGGGCTG GCATTTGTGA ATGAGGACAT CGTGGCCTCC 
AAGGGGAGCG GCCTGGGCAC CATCTGCCTG TGGAGCTGGA GGCAGACGTG GGGGGGCCGG 
GGCAGCCAGT CCACGGTGGC AGTGGTGGTC CTGGCGCGGC TGCAATGGTC GTCCACCGAG 
TTGGCCTACT TCTCGCTCAG CGCCTGCCCT GATAAGGGGA TTGTGCTCTG TGGGGATGAG 
GAGGGCAACG TGTGGCTCTA CGACGTCAGC AACATCCTGA AGCAGCCACC CCTGCTGCCG 
GCAGCCCTGC AGGCCCCCAC ACAGATCCTG AAGTGGCCCC AGCCCTGGGC CCTTGGCCAG 
GTGGTGACCA AGACCATGGT GAACACAGTG GTGGCCAATG CCTCCTTCAC CTACCTCACC 
GCCCTGACGG ACTCCAACAT CGTAGCCATC TGGGGGAGGA TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

LRWD1 Regulates Microtubule Nucleation and Proper Cell Cycle Progression in the Human Testicular Embryonic Carcinoma Cells.
Journal of cellular biochemistry119(1)314-326(2018 Jan)
Wang CY,Hong YH,Syu JS,Tsai YC,Liu XY,Chen TY,Su YM,Kuo PL,Lin YM,Teng YN

High-throughput RNAi screen in Ewing sarcoma cells identifies leucine rich repeats and WD repeat domain containing 1 (LRWD1) as a regulator of EWS-FLI1 driven cell viability.
Gene596137-146(2017 Jan)
He T,Surdez D,Rantala JK,Haapa-Paananen S,Ban J,Kauer M,Tomazou E,Fey V,Alonso J,Kovar H,Delattre O,Iljin K

The preRC protein ORCA organizes heterochromatin by assembling histone H3 lysine 9 methyltransferases on chromatin.
eLife4(2015 Apr)
Giri S,Aggarwal V,Pontis J,Shen Z,Chakraborty A,Khan A,Mizzen C,Prasanth KV,Ait-Si-Ali S,Ha T,Prasanth SG

Correlation between leucine rich domain and the stability of LRWD1 protein in human NT2/D1 cells.
Advances in medical sciences59(2)266-72(2014 Sep)
Tsai YC,Teng YN,Hung JH,Wu CH,Kuo YT,Kuo PL,Chiu CC,Huang B

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LRWD1 cDNA ORF clone, Homo sapiens(human)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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