CES5A cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	CES5A
Entrez Gene ID	221223
Full Name	carboxylesterase 5A
Synonyms	CAUXIN,CES4C1,CES5,CES7,HEL126
General protein information	Preferred Names carboxylesterase 5A Names carboxylesterase 5A carboxylesterase 5 carboxylesterase 7 carboxylesterase-like urinary excreted protein homolog epididymis luminal protein 126
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 16 Map Location: 16q12.2
Summary	This gene encodes a member of the carboxylesterase large family. The family members are responsible for the hydrolysis or transesterification of various xenobiotics, such as cocaine and heroin, and endogenous substrates with ester, thioester, or amide bonds. They also participate in fatty acyl and cholesterol ester metabolism, and may play a role in the blood-brain barrier system. This gene, also called CES5, is predominantly expressed in peripheral tissues, including brain, kidney, lung and testis. It encodes a secreted enzyme. Because of high levels in the urine of male domestic cats, this enzyme is also called cauxin (carboxylesterase-like urinary excreted protein). The enzyme functions in regulating the production of a pheromone precursor and may contribute to lipid and cholesterol transfer processes within male reproductive fluids. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2010].

mRNA and Protein(s)

mRNA	Protein	Name
NM_145024.3	NP_659461.1	carboxylesterase 5A isoform 2 precursor
NM_001143685.2	NP_001137157.1	carboxylesterase 5A isoform 1 precursor
NM_001143685.1	NP_001137157.1	carboxylesterase 5A isoform 1 precursor
NM_145024.2	NP_659461.1	carboxylesterase 5A isoform 2 precursor
NM_001190158.1	NP_001177087.1	carboxylesterase 5A isoform 3 precursor

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Ces5a	NP_001003951.1
Rattus norvegicus (Norway rat)	Ces5a	NP_001012056.1
	CES5A	NP_001161966.1
Canis lupus familiaris (dog)	CES5A	NP_001003969.1
Bos taurus (cattle)	CES5A	NP_001179288.1
Homo sapiens (human)	CES5A	NP_001137157.1
Pan troglodytes (chimpanzee)	CES5A	XP_003315150.1

Gene Ontology
Bibliography

Related articles in PubMed

Genome wide association study (GWAS) of Chagas cardiomyopathy in Trypanosoma cruzi seropositive subjects.
Deng X, Sabino EC, Cunha-Neto E, Ribeiro AL, Ianni B, Mady C, Busch MP, Seielstad M,
PloS one8(11)e79629(2013)

Recommended nomenclature for five mammalian carboxylesterase gene families: human, mouse, and rat genes and proteins.
Holmes RS, Wright MW, Laulederkind SJ, Cox LA, Hosokawa M, Imai T, Ishibashi S, Lehner R, Miyazaki M, Perkins EJ, Potter PM, Redinbo MR, Robert J, Satoh T, Yamashita T, Yan B, Yokoi T, Zechner R, Maltais LJ
Mammalian genome : official journal of the International Mammalian Genome Society21(9-10)427-41(2010 Oct)

Mammalian carboxylesterase 3: comparative genomics and proteomics.
Holmes RS, Cox LA, VandeBerg JL
Genetica138(7)695-708(2010 Jul)

A new class of mammalian carboxylesterase CES6.
Holmes RS, Cox LA, Vandeberg JL
Comparative biochemistry and physiology. Part D, Genomics & proteomics4(3)209-17(2009 Sep)

Mammalian carboxylesterase 5: comparative biochemistry and genomics.
Holmes RS, Cox LA, Vandeberg JL
Comparative biochemistry and physiology. Part D, Genomics & proteomics3(3)195-204(2008 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Clinical trial of gene-disease association and gene-environment interaction. (HuGE Navigator)
Title: Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score.

The following CES5A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CES5A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu09824	NM_001190158.1 Latest version!	Homo sapiens carboxylesterase 5A (CES5A), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$219.50-$307.30 $439.00
OHu31783	NM_001143685.2 Latest version!	Homo sapiens carboxylesterase 5A (CES5A), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$219.50-$307.30 $439.00
OHu06445	NM_145024.3 Latest version!	Homo sapiens carboxylesterase 5A (CES5A), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
View Old Accession Versions >>
OHu31783	NM_001143685.1	Homo sapiens carboxylesterase 5A (CES5A), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$219.50-$307.30 $439.00
OHu06445	NM_145024.2	Homo sapiens carboxylesterase 5A (CES5A), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CES5A ( NM_001190158.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001177087.1 Homo sapiens carboxylesterase 5A (CES5A), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu09824
Clone ID Related Accession (Same CDS sequence)	NM_001190158.1
Accession Version	NM_001190158.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1815bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Homo sapiens(human)
Product	carboxylesterase 5A isoform 3 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC007335.7, AK294334.1 and AC147362.2. Transcript Variant: This variant (3) encodes the longest isoform (3). ##Evidence-Data-START## Transcript exon combination :: AK294334.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1968968, SAMEA2144335 [ECO:0000350] ##Evidence-Data-END##

ATGGCTGTTC TGGTTTGCCC TGCTTCATGC CATGGCCTAA AGGAGTTTAG GATAAGAAGG 
GGAATGTGGA GATTGTGCCT GGTTTACTAT TTTTATCCAG CCTCCTCAAC TTTGTATGTA 
TTGAGGATTG ATGTCTTAAA CTACACTTCC AAAGATGAGG GGCCTTCTGC TGAAGGGCCA 
CAGAGGAACA CCAGGCTGGG ATGGATTCAG GGCAAGCAAG TCACTGTGCT GGGAAGCCCT 
GTGCCTGTGA ACGTGTTCCT CGGAGTCCCC TTTGCTGCTC CCCCGCTGGG ATCCCTGCGA 
TTTACGAACC CGCAGCCTGC ATCGCCCTGG GATAACTTGC GAGAAGCCAC CTCCTACCCT 
AATTTGTGCC TCCAGAACTC AGAGTGGCTG CTCTTAGATC AACATATGCT CAAGGTGCAT 
TACCCGAAAT TCGGAGTGTC AGAAGACTGC CTCTACCTGA ACATCTATGC GCCTGCCCAC 
GCCGATACAG GCTCCAAGCT CCCCGTCTTG GTGTGGTTCC CAGGAGGTGC CTTCAAGACT 
GGCTCAGCCT CCATCTTTGA TGGGTCCGCC CTGGCTGCCT ATGAGGACGT GCTGGTTGTG 
GTCGTCCAGT ACCGGCTAGG AATATTTGGT TTCTTCACCA CATGGGATCA GCATGCTCCG 
GGGAACTGGG CCTTCAAGGA CCAGGTGGCT GCTCTGTCCT GGGTCCAGAA GAACATCGAG 
TTCTTCGGTG GGGACCCCAG CTCTGTGACC ATCTTTGGCG AGTCCGCGGG AGCCATAAGT 
GTTTCTAGTC TTATACTGTC TCCCATGGCC AAAGGCTTAT TCCACAAAGC CATCATGGAG 
AGTGGGGTGG CCATCATCCC TTACCTGGAG GCCCATGATT ATGAGAAGAG TGAGGACCTG 
CAGGTGGTTG CACATTTCTG TGGTAACAAT GCGTCAGACT CTGAGGCCCT GCTGAGGTGC 
CTGAGGACAA AACCCTCCAA GGAGCTGCTG ACCCTCAGCC AGAAAACAAA GTCTTTCACT 
CGAGTGGTTG ATGGTGCTTT CTTTCCTAAT GAGCCTCTAG ATCTATTGTC TCAGAAAGCA 
TTTAAAGCAA TTCCTTCCAT CATCGGAGTC AATAACCACG AGTGTGGCTT CCTGCTGCCT 
ATGAAGGAGG CTCCTGAGAT CCTCAGTGGC TCCAACAAGT CCCTTGCCCT CCATCTGATA 
CAAAACATCC TGCACATCCC GCCTCAGTAT TTGCACCTTG TGGCTAATGA ATACTTCCAT 
GACAAGCACT CCCTGACTGA AATCCGAGAC AGTCTTCTGG ACTTGCTTGG AGATGTGTTC 
TTTGTGGTCC CTGCACTGAT CACAGCTCGA TATCACAGAG ATGCTGGTGC ACCTGTCTAC 
TTCTATGAGT TTCGGCACCG GCCTCAGTGC TTTGAAGACA CGAAGCCAGC TTTTGTCAAA 
GCCGACCACG CTGATGAAGT CCGCTTTGTG TTCGGTGGTG CCTTCCTGAA GGGGGACATT 
GTTATGTTCG AAGGAGCCAC GGAGGAGGAG AAGTTACTGA GCCGGAAGAT GATGAAATAC 
TGGGCTACCT TTGCTCGAAC CGGGAATCCT AATGGGAACG ACCTGTCTCT GTGGCCAGCT 
TATAATCTGA CTGAGCAGTA CCTCCAGCTG GACTTGAACA TGAGCCTCGG ACAGAGACTC 
AAAGAACCGC GGGTGGATTT TTGGACCAGC ACCATCCCCC TGATCCTGTC TGCCTCCGAC 
ATGCTCCACA GTCCTCTTTC TTCCTTAACT TTCCTCTCTC TCCTCCAGCC TTTCTTTTTC 
TTTTGTGCTC CTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001177087.1
CDS	147..1961
Translation	MAVLVCPASCHGLKEFRIRRGMWRLCLVYYFYPASSTLYVLRIDVLNYTSKDEGPSAEGPQRNTRLGWIQGKQVTVLGSPVPVNVFLGVPFAAPPLGSLRFTNPQPASPWDNLREATSYPNLCLQNSEWLLLDQHMLKVHYPKFGVSEDCLYLNIYAPAHADTGSKLPVLVWFPGGAFKTGSASIFDGSALAAYEDVLVVVVQYRLGIFGFFTTWDQHAPGNWAFKDQVAALSWVQKNIEFFGGDPSSVTIFGESAGAISVSSLILSPMAKGLFHKAIMESGVAIIPYLEAHDYEKSEDLQVVAHFCGNNASDSEALLRCLRTKPSKELLTLSQKTKSFTRVVDGAFFPNEPLDLLSQKAFKAIPSIIGVNNHECGFLLPMKEAPEILSGSNKSLALHLIQNILHIPPQYLHLVANEYFHDKHSLTEIRDSLLDLLGDVFFVVPALITARYHRDAGAPVYFYEFRHRPQCFEDTKPAFVKADHADEVRFVFGGAFLKGDIVMFEGATEEEKLLSRKMMKYWATFARTGNPNGNDLSLWPAYNLTEQYLQLDLNMSLGQRLKEPRVDFWTSTIPLILSASDMLHSPLSSLTFLSLLQPFFFFCAP

Target ORF information:

RefSeq Version	NM_001190158.1
Organism	Homo sapiens(human)
Definition	Homo sapiens carboxylesterase 5A (CES5A), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001190158.1

ORF Insert Sequence:

ATGGCTGTTC TGGTTTGCCC TGCTTCATGC CATGGCCTAA AGGAGTTTAG GATAAGAAGG 
GGAATGTGGA GATTGTGCCT GGTTTACTAT TTTTATCCAG CCTCCTCAAC TTTGTATGTA 
TTGAGGATTG ATGTCTTAAA CTACACTTCC AAAGATGAGG GGCCTTCTGC TGAAGGGCCA 
CAGAGGAACA CCAGGCTGGG ATGGATTCAG GGCAAGCAAG TCACTGTGCT GGGAAGCCCT 
GTGCCTGTGA ACGTGTTCCT CGGAGTCCCC TTTGCTGCTC CCCCGCTGGG ATCCCTGCGA 
TTTACGAACC CGCAGCCTGC ATCGCCCTGG GATAACTTGC GAGAAGCCAC CTCCTACCCT 
AATTTGTGCC TCCAGAACTC AGAGTGGCTG CTCTTAGATC AACATATGCT CAAGGTGCAT 
TACCCGAAAT TCGGAGTGTC AGAAGACTGC CTCTACCTGA ACATCTATGC GCCTGCCCAC 
GCCGATACAG GCTCCAAGCT CCCCGTCTTG GTGTGGTTCC CAGGAGGTGC CTTCAAGACT 
GGCTCAGCCT CCATCTTTGA TGGGTCCGCC CTGGCTGCCT ATGAGGACGT GCTGGTTGTG 
GTCGTCCAGT ACCGGCTAGG AATATTTGGT TTCTTCACCA CATGGGATCA GCATGCTCCG 
GGGAACTGGG CCTTCAAGGA CCAGGTGGCT GCTCTGTCCT GGGTCCAGAA GAACATCGAG 
TTCTTCGGTG GGGACCCCAG CTCTGTGACC ATCTTTGGCG AGTCCGCGGG AGCCATAAGT 
GTTTCTAGTC TTATACTGTC TCCCATGGCC AAAGGCTTAT TCCACAAAGC CATCATGGAG 
AGTGGGGTGG CCATCATCCC TTACCTGGAG GCCCATGATT ATGAGAAGAG TGAGGACCTG 
CAGGTGGTTG CACATTTCTG TGGTAACAAT GCGTCAGACT CTGAGGCCCT GCTGAGGTGC 
CTGAGGACAA AACCCTCCAA GGAGCTGCTG ACCCTCAGCC AGAAAACAAA GTCTTTCACT 
CGAGTGGTTG ATGGTGCTTT CTTTCCTAAT GAGCCTCTAG ATCTATTGTC TCAGAAAGCA 
TTTAAAGCAA TTCCTTCCAT CATCGGAGTC AATAACCACG AGTGTGGCTT CCTGCTGCCT 
ATGAAGGAGG CTCCTGAGAT CCTCAGTGGC TCCAACAAGT CCCTTGCCCT CCATCTGATA 
CAAAACATCC TGCACATCCC GCCTCAGTAT TTGCACCTTG TGGCTAATGA ATACTTCCAT 
GACAAGCACT CCCTGACTGA AATCCGAGAC AGTCTTCTGG ACTTGCTTGG AGATGTGTTC 
TTTGTGGTCC CTGCACTGAT CACAGCTCGA TATCACAGAG ATGCTGGTGC ACCTGTCTAC 
TTCTATGAGT TTCGGCACCG GCCTCAGTGC TTTGAAGACA CGAAGCCAGC TTTTGTCAAA 
GCCGACCACG CTGATGAAGT CCGCTTTGTG TTCGGTGGTG CCTTCCTGAA GGGGGACATT 
GTTATGTTCG AAGGAGCCAC GGAGGAGGAG AAGTTACTGA GCCGGAAGAT GATGAAATAC 
TGGGCTACCT TTGCTCGAAC CGGGAATCCT AATGGGAACG ACCTGTCTCT GTGGCCAGCT 
TATAATCTGA CTGAGCAGTA CCTCCAGCTG GACTTGAACA TGAGCCTCGG ACAGAGACTC 
AAAGAACCGC GGGTGGATTT TTGGACCAGC ACCATCCCCC TGATCCTGTC TGCCTCCGAC 
ATGCTCCACA GTCCTCTTTC TTCCTTAACT TTCCTCTCTC TCCTCCAGCC TTTCTTTTTC 
TTTTGTGCTC CTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CES5A ( NM_001143685.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001137157.1 Homo sapiens carboxylesterase 5A (CES5A), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu31783
Clone ID Related Accession (Same CDS sequence)	NM_001143685.2 , NM_001143685.1
Accession Version	NM_001143685.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1728bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-21
Organism	Homo sapiens(human)
Product	carboxylesterase 5A isoform 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC147362.2, DA698987.1, BC117126.1, BC069548.1, AY907669.1 and BC039073.1. On May 17, 2019 this sequence version replaced NM_001143685.1. Transcript Variant: This variant (1) has an alternate 5' sequence, as compared to variant 3. The resulting isoform (1) is shorter and has a different N-terminus, as compared to isoform 3. ##Evidence-Data-START## Transcript exon combination :: BC069501.1, AY907669.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1968968, SAMEA2144335 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000290567.14/ ENSP00000290567.9 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END##

ATGAGTGGGA ATTGGGTGCA CCCAGGCCAG ATCCTAATTT GGGCTATCTG GGTCCTTGCA 
GCCCCCACCA AAGGGCCTTC TGCTGAAGGG CCACAGAGGA ACACCAGGCT GGGATGGATT 
CAGGGCAAGC AAGTCACTGT GCTGGGAAGC CCTGTGCCTG TGAACGTGTT CCTCGGAGTC 
CCCTTTGCTG CTCCCCCGCT GGGATCCCTG CGATTTACGA ACCCGCAGCC TGCATCGCCC 
TGGGATAACT TGCGAGAAGC CACCTCCTAC CCTAATTTGT GCCTCCAGAA CTCAGAGTGG 
CTGCTCTTAG ATCAACATAT GCTCAAGGTG CATTACCCGA AATTCGGAGT GTCAGAAGAC 
TGCCTCTACC TGAACATCTA TGCGCCTGCC CACGCCGATA CAGGCTCCAA GCTCCCCGTC 
TTGGTGTGGT TCCCAGGAGG TGCCTTCAAG ACTGGCTCAG CCTCCATCTT TGATGGGTCC 
GCCCTGGCTG CCTATGAGGA CGTGCTGGTT GTGGTCGTCC AGTACCGGCT AGGAATATTT 
GGTTTCTTCA CCACATGGGA TCAGCATGCT CCGGGGAACT GGGCCTTCAA GGACCAGGTG 
GCTGCTCTGT CCTGGGTCCA GAAGAACATC GAGTTCTTCG GTGGGGACCC CAGCTCTGTG 
ACCATCTTTG GCGAGTCCGC GGGAGCCATA AGTGTTTCTA GTCTTATACT GTCTCCCATG 
GCCAAAGGCT TATTCCACAA AGCCATCATG GAGAGTGGGG TGGCCATCAT CCCTTACCTG 
GAGGCCCATG ATTATGAGAA GAGTGAGGAC CTGCAGGTGG TTGCACATTT CTGTGGTAAC 
AATGCGTCAG ACTCTGAGGC CCTGCTGAGG TGCCTGAGGA CAAAACCCTC CAAGGAGCTG 
CTGACCCTCA GCCAGAAAAC AAAGTCTTTC ACTCGAGTGG TTGATGGTGC TTTCTTTCCT 
AATGAGCCTC TAGATCTATT GTCTCAGAAA GCATTTAAAG CAATTCCTTC CATCATCGGA 
GTCAATAACC ACGAGTGTGG CTTCCTGCTG CCTATGAAGG AGGCTCCTGA GATCCTCAGT 
GGCTCCAACA AGTCCCTTGC CCTCCATCTG ATACAAAACA TCCTGCACAT CCCGCCTCAG 
TATTTGCACC TTGTGGCTAA TGAATACTTC CATGACAAGC ACTCCCTGAC TGAAATCCGA 
GACAGTCTTC TGGACTTGCT TGGAGATGTG TTCTTTGTGG TCCCTGCACT GATCACAGCT 
CGATATCACA GAGATGCTGG TGCACCTGTC TACTTCTATG AGTTTCGGCA CCGGCCTCAG 
TGCTTTGAAG ACACGAAGCC AGCTTTTGTC AAAGCCGACC ACGCTGATGA AGTCCGCTTT 
GTGTTCGGTG GTGCCTTCCT GAAGGGGGAC ATTGTTATGT TCGAAGGAGC CACGGAGGAG 
GAGAAGTTAC TGAGCCGGAA GATGATGAAA TACTGGGCTA CCTTTGCTCG AACCGGGAAT 
CCTAATGGGA ACGACCTGTC TCTGTGGCCA GCTTATAATC TGACTGAGCA GTACCTCCAG 
CTGGACTTGA ACATGAGCCT CGGACAGAGA CTCAAAGAAC CGCGGGTGGA TTTTTGGACC 
AGCACCATCC CCCTGATCCT GTCTGCCTCC GACATGCTCC ACAGTCCTCT TTCTTCCTTA 
ACTTTCCTCT CTCTCCTCCA GCCTTTCTTT TTCTTTTGTG CTCCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001137157.1
CDS	153..1880
Translation	MSGNWVHPGQILIWAIWVLAAPTKGPSAEGPQRNTRLGWIQGKQVTVLGSPVPVNVFLGVPFAAPPLGSLRFTNPQPASPWDNLREATSYPNLCLQNSEWLLLDQHMLKVHYPKFGVSEDCLYLNIYAPAHADTGSKLPVLVWFPGGAFKTGSASIFDGSALAAYEDVLVVVVQYRLGIFGFFTTWDQHAPGNWAFKDQVAALSWVQKNIEFFGGDPSSVTIFGESAGAISVSSLILSPMAKGLFHKAIMESGVAIIPYLEAHDYEKSEDLQVVAHFCGNNASDSEALLRCLRTKPSKELLTLSQKTKSFTRVVDGAFFPNEPLDLLSQKAFKAIPSIIGVNNHECGFLLPMKEAPEILSGSNKSLALHLIQNILHIPPQYLHLVANEYFHDKHSLTEIRDSLLDLLGDVFFVVPALITARYHRDAGAPVYFYEFRHRPQCFEDTKPAFVKADHADEVRFVFGGAFLKGDIVMFEGATEEEKLLSRKMMKYWATFARTGNPNGNDLSLWPAYNLTEQYLQLDLNMSLGQRLKEPRVDFWTSTIPLILSASDMLHSPLSSLTFLSLLQPFFFFCAP

Target ORF information:

RefSeq Version	NM_001143685.2
Organism	Homo sapiens(human)
Definition	Homo sapiens carboxylesterase 5A (CES5A), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001143685.2

ORF Insert Sequence:

ATGAGTGGGA ATTGGGTGCA CCCAGGCCAG ATCCTAATTT GGGCTATCTG GGTCCTTGCA 
GCCCCCACCA AAGGGCCTTC TGCTGAAGGG CCACAGAGGA ACACCAGGCT GGGATGGATT 
CAGGGCAAGC AAGTCACTGT GCTGGGAAGC CCTGTGCCTG TGAACGTGTT CCTCGGAGTC 
CCCTTTGCTG CTCCCCCGCT GGGATCCCTG CGATTTACGA ACCCGCAGCC TGCATCGCCC 
TGGGATAACT TGCGAGAAGC CACCTCCTAC CCTAATTTGT GCCTCCAGAA CTCAGAGTGG 
CTGCTCTTAG ATCAACATAT GCTCAAGGTG CATTACCCGA AATTCGGAGT GTCAGAAGAC 
TGCCTCTACC TGAACATCTA TGCGCCTGCC CACGCCGATA CAGGCTCCAA GCTCCCCGTC 
TTGGTGTGGT TCCCAGGAGG TGCCTTCAAG ACTGGCTCAG CCTCCATCTT TGATGGGTCC 
GCCCTGGCTG CCTATGAGGA CGTGCTGGTT GTGGTCGTCC AGTACCGGCT AGGAATATTT 
GGTTTCTTCA CCACATGGGA TCAGCATGCT CCGGGGAACT GGGCCTTCAA GGACCAGGTG 
GCTGCTCTGT CCTGGGTCCA GAAGAACATC GAGTTCTTCG GTGGGGACCC CAGCTCTGTG 
ACCATCTTTG GCGAGTCCGC GGGAGCCATA AGTGTTTCTA GTCTTATACT GTCTCCCATG 
GCCAAAGGCT TATTCCACAA AGCCATCATG GAGAGTGGGG TGGCCATCAT CCCTTACCTG 
GAGGCCCATG ATTATGAGAA GAGTGAGGAC CTGCAGGTGG TTGCACATTT CTGTGGTAAC 
AATGCGTCAG ACTCTGAGGC CCTGCTGAGG TGCCTGAGGA CAAAACCCTC CAAGGAGCTG 
CTGACCCTCA GCCAGAAAAC AAAGTCTTTC ACTCGAGTGG TTGATGGTGC TTTCTTTCCT 
AATGAGCCTC TAGATCTATT GTCTCAGAAA GCATTTAAAG CAATTCCTTC CATCATCGGA 
GTCAATAACC ACGAGTGTGG CTTCCTGCTG CCTATGAAGG AGGCTCCTGA GATCCTCAGT 
GGCTCCAACA AGTCCCTTGC CCTCCATCTG ATACAAAACA TCCTGCACAT CCCGCCTCAG 
TATTTGCACC TTGTGGCTAA TGAATACTTC CATGACAAGC ACTCCCTGAC TGAAATCCGA 
GACAGTCTTC TGGACTTGCT TGGAGATGTG TTCTTTGTGG TCCCTGCACT GATCACAGCT 
CGATATCACA GAGATGCTGG TGCACCTGTC TACTTCTATG AGTTTCGGCA CCGGCCTCAG 
TGCTTTGAAG ACACGAAGCC AGCTTTTGTC AAAGCCGACC ACGCTGATGA AGTCCGCTTT 
GTGTTCGGTG GTGCCTTCCT GAAGGGGGAC ATTGTTATGT TCGAAGGAGC CACGGAGGAG 
GAGAAGTTAC TGAGCCGGAA GATGATGAAA TACTGGGCTA CCTTTGCTCG AACCGGGAAT 
CCTAATGGGA ACGACCTGTC TCTGTGGCCA GCTTATAATC TGACTGAGCA GTACCTCCAG 
CTGGACTTGA ACATGAGCCT CGGACAGAGA CTCAAAGAAC CGCGGGTGGA TTTTTGGACC 
AGCACCATCC CCCTGATCCT GTCTGCCTCC GACATGCTCC ACAGTCCTCT TTCTTCCTTA 
ACTTTCCTCT CTCTCCTCCA GCCTTTCTTT TTCTTTTGTG CTCCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CES5A ( NM_001143685.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001137157.1 Homo sapiens carboxylesterase 5A (CES5A), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu31783
Clone ID Related Accession (Same CDS sequence)	NM_001143685.2 , NM_001143685.1
Accession Version	NM_001143685.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1728bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-19
Organism	Homo sapiens(human)
Product	carboxylesterase 5A isoform 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA698987.1, BC117126.1, BC069548.1, AC147362.2, AY907669.1 and BC039073.1. Transcript Variant: This variant (1) has an alternate 5' sequence, as compared to variant 3. The resulting isoform (1) is shorter and has a different N-terminus, as compared to isoform 3. ##Evidence-Data-START## Transcript exon combination :: BC069501.1, AY907669.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1968968, SAMEA2144335 [ECO:0000350] ##Evidence-Data-END##

ATGAGTGGGA ATTGGGTGCA CCCAGGCCAG ATCCTAATTT GGGCTATCTG GGTCCTTGCA 
GCCCCCACCA AAGGGCCTTC TGCTGAAGGG CCACAGAGGA ACACCAGGCT GGGATGGATT 
CAGGGCAAGC AAGTCACTGT GCTGGGAAGC CCTGTGCCTG TGAACGTGTT CCTCGGAGTC 
CCCTTTGCTG CTCCCCCGCT GGGATCCCTG CGATTTACGA ACCCGCAGCC TGCATCGCCC 
TGGGATAACT TGCGAGAAGC CACCTCCTAC CCTAATTTGT GCCTCCAGAA CTCAGAGTGG 
CTGCTCTTAG ATCAACATAT GCTCAAGGTG CATTACCCGA AATTCGGAGT GTCAGAAGAC 
TGCCTCTACC TGAACATCTA TGCGCCTGCC CACGCCGATA CAGGCTCCAA GCTCCCCGTC 
TTGGTGTGGT TCCCAGGAGG TGCCTTCAAG ACTGGCTCAG CCTCCATCTT TGATGGGTCC 
GCCCTGGCTG CCTATGAGGA CGTGCTGGTT GTGGTCGTCC AGTACCGGCT AGGAATATTT 
GGTTTCTTCA CCACATGGGA TCAGCATGCT CCGGGGAACT GGGCCTTCAA GGACCAGGTG 
GCTGCTCTGT CCTGGGTCCA GAAGAACATC GAGTTCTTCG GTGGGGACCC CAGCTCTGTG 
ACCATCTTTG GCGAGTCCGC GGGAGCCATA AGTGTTTCTA GTCTTATACT GTCTCCCATG 
GCCAAAGGCT TATTCCACAA AGCCATCATG GAGAGTGGGG TGGCCATCAT CCCTTACCTG 
GAGGCCCATG ATTATGAGAA GAGTGAGGAC CTGCAGGTGG TTGCACATTT CTGTGGTAAC 
AATGCGTCAG ACTCTGAGGC CCTGCTGAGG TGCCTGAGGA CAAAACCCTC CAAGGAGCTG 
CTGACCCTCA GCCAGAAAAC AAAGTCTTTC ACTCGAGTGG TTGATGGTGC TTTCTTTCCT 
AATGAGCCTC TAGATCTATT GTCTCAGAAA GCATTTAAAG CAATTCCTTC CATCATCGGA 
GTCAATAACC ACGAGTGTGG CTTCCTGCTG CCTATGAAGG AGGCTCCTGA GATCCTCAGT 
GGCTCCAACA AGTCCCTTGC CCTCCATCTG ATACAAAACA TCCTGCACAT CCCGCCTCAG 
TATTTGCACC TTGTGGCTAA TGAATACTTC CATGACAAGC ACTCCCTGAC TGAAATCCGA 
GACAGTCTTC TGGACTTGCT TGGAGATGTG TTCTTTGTGG TCCCTGCACT GATCACAGCT 
CGATATCACA GAGATGCTGG TGCACCTGTC TACTTCTATG AGTTTCGGCA CCGGCCTCAG 
TGCTTTGAAG ACACGAAGCC AGCTTTTGTC AAAGCCGACC ACGCTGATGA AGTCCGCTTT 
GTGTTCGGTG GTGCCTTCCT GAAGGGGGAC ATTGTTATGT TCGAAGGAGC CACGGAGGAG 
GAGAAGTTAC TGAGCCGGAA GATGATGAAA TACTGGGCTA CCTTTGCTCG AACCGGGAAT 
CCTAATGGGA ACGACCTGTC TCTGTGGCCA GCTTATAATC TGACTGAGCA GTACCTCCAG 
CTGGACTTGA ACATGAGCCT CGGACAGAGA CTCAAAGAAC CGCGGGTGGA TTTTTGGACC 
AGCACCATCC CCCTGATCCT GTCTGCCTCC GACATGCTCC ACAGTCCTCT TTCTTCCTTA 
ACTTTCCTCT CTCTCCTCCA GCCTTTCTTT TTCTTTTGTG CTCCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001137157.1
CDS	150..1877
Translation	MSGNWVHPGQILIWAIWVLAAPTKGPSAEGPQRNTRLGWIQGKQVTVLGSPVPVNVFLGVPFAAPPLGSLRFTNPQPASPWDNLREATSYPNLCLQNSEWLLLDQHMLKVHYPKFGVSEDCLYLNIYAPAHADTGSKLPVLVWFPGGAFKTGSASIFDGSALAAYEDVLVVVVQYRLGIFGFFTTWDQHAPGNWAFKDQVAALSWVQKNIEFFGGDPSSVTIFGESAGAISVSSLILSPMAKGLFHKAIMESGVAIIPYLEAHDYEKSEDLQVVAHFCGNNASDSEALLRCLRTKPSKELLTLSQKTKSFTRVVDGAFFPNEPLDLLSQKAFKAIPSIIGVNNHECGFLLPMKEAPEILSGSNKSLALHLIQNILHIPPQYLHLVANEYFHDKHSLTEIRDSLLDLLGDVFFVVPALITARYHRDAGAPVYFYEFRHRPQCFEDTKPAFVKADHADEVRFVFGGAFLKGDIVMFEGATEEEKLLSRKMMKYWATFARTGNPNGNDLSLWPAYNLTEQYLQLDLNMSLGQRLKEPRVDFWTSTIPLILSASDMLHSPLSSLTFLSLLQPFFFFCAP

Target ORF information:

RefSeq Version	NM_001143685.1
Organism	Homo sapiens(human)
Definition	Homo sapiens carboxylesterase 5A (CES5A), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001143685.1

ORF Insert Sequence:

ATGAGTGGGA ATTGGGTGCA CCCAGGCCAG ATCCTAATTT GGGCTATCTG GGTCCTTGCA 
GCCCCCACCA AAGGGCCTTC TGCTGAAGGG CCACAGAGGA ACACCAGGCT GGGATGGATT 
CAGGGCAAGC AAGTCACTGT GCTGGGAAGC CCTGTGCCTG TGAACGTGTT CCTCGGAGTC 
CCCTTTGCTG CTCCCCCGCT GGGATCCCTG CGATTTACGA ACCCGCAGCC TGCATCGCCC 
TGGGATAACT TGCGAGAAGC CACCTCCTAC CCTAATTTGT GCCTCCAGAA CTCAGAGTGG 
CTGCTCTTAG ATCAACATAT GCTCAAGGTG CATTACCCGA AATTCGGAGT GTCAGAAGAC 
TGCCTCTACC TGAACATCTA TGCGCCTGCC CACGCCGATA CAGGCTCCAA GCTCCCCGTC 
TTGGTGTGGT TCCCAGGAGG TGCCTTCAAG ACTGGCTCAG CCTCCATCTT TGATGGGTCC 
GCCCTGGCTG CCTATGAGGA CGTGCTGGTT GTGGTCGTCC AGTACCGGCT AGGAATATTT 
GGTTTCTTCA CCACATGGGA TCAGCATGCT CCGGGGAACT GGGCCTTCAA GGACCAGGTG 
GCTGCTCTGT CCTGGGTCCA GAAGAACATC GAGTTCTTCG GTGGGGACCC CAGCTCTGTG 
ACCATCTTTG GCGAGTCCGC GGGAGCCATA AGTGTTTCTA GTCTTATACT GTCTCCCATG 
GCCAAAGGCT TATTCCACAA AGCCATCATG GAGAGTGGGG TGGCCATCAT CCCTTACCTG 
GAGGCCCATG ATTATGAGAA GAGTGAGGAC CTGCAGGTGG TTGCACATTT CTGTGGTAAC 
AATGCGTCAG ACTCTGAGGC CCTGCTGAGG TGCCTGAGGA CAAAACCCTC CAAGGAGCTG 
CTGACCCTCA GCCAGAAAAC AAAGTCTTTC ACTCGAGTGG TTGATGGTGC TTTCTTTCCT 
AATGAGCCTC TAGATCTATT GTCTCAGAAA GCATTTAAAG CAATTCCTTC CATCATCGGA 
GTCAATAACC ACGAGTGTGG CTTCCTGCTG CCTATGAAGG AGGCTCCTGA GATCCTCAGT 
GGCTCCAACA AGTCCCTTGC CCTCCATCTG ATACAAAACA TCCTGCACAT CCCGCCTCAG 
TATTTGCACC TTGTGGCTAA TGAATACTTC CATGACAAGC ACTCCCTGAC TGAAATCCGA 
GACAGTCTTC TGGACTTGCT TGGAGATGTG TTCTTTGTGG TCCCTGCACT GATCACAGCT 
CGATATCACA GAGATGCTGG TGCACCTGTC TACTTCTATG AGTTTCGGCA CCGGCCTCAG 
TGCTTTGAAG ACACGAAGCC AGCTTTTGTC AAAGCCGACC ACGCTGATGA AGTCCGCTTT 
GTGTTCGGTG GTGCCTTCCT GAAGGGGGAC ATTGTTATGT TCGAAGGAGC CACGGAGGAG 
GAGAAGTTAC TGAGCCGGAA GATGATGAAA TACTGGGCTA CCTTTGCTCG AACCGGGAAT 
CCTAATGGGA ACGACCTGTC TCTGTGGCCA GCTTATAATC TGACTGAGCA GTACCTCCAG 
CTGGACTTGA ACATGAGCCT CGGACAGAGA CTCAAAGAAC CGCGGGTGGA TTTTTGGACC 
AGCACCATCC CCCTGATCCT GTCTGCCTCC GACATGCTCC ACAGTCCTCT TTCTTCCTTA 
ACTTTCCTCT CTCTCCTCCA GCCTTTCTTT TTCTTTTGTG CTCCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CES5A ( NM_145024.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_659461.1 Homo sapiens carboxylesterase 5A (CES5A), transcript variant 2, mRNA.

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CloneID	OHu06445
Clone ID Related Accession (Same CDS sequence)	NM_145024.3 , NM_145024.2
Accession Version	NM_145024.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1578bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-21
Organism	Homo sapiens(human)
Product	carboxylesterase 5A isoform 2 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC147362.2, AK056109.1 and BC039073.1. On May 31, 2019 this sequence version replaced NM_145024.2. Transcript Variant: This variant (2) has an alternate 5' sequence, and omits an in-frame exon in the 3' CDS, as compared to variant 3. The resulting isoform (2) is shorter; has a different N-terminus and lacks an internal segment, as compared to isoform 3. ##Evidence-Data-START## Transcript exon combination :: EU794678.1, AK056109.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1968968, SAMEA2144335 [ECO:0000350] ##Evidence-Data-END##

ATGAGTGGGA ATTGGGTGCA CCCAGGCCAG ATCCTAATTT GGGCTATCTG GGTCCTTGCA 
GCCCCCACCA AAGGGCCTTC TGCTGAAGGG CCACAGAGGA ACACCAGGCT GGGATGGATT 
CAGGGCAAGC AAGTCACTGT GCTGGGAAGC CCTGTGCCTG TGAACGTGTT CCTCGGAGTC 
CCCTTTGCTG CTCCCCCGCT GGGATCCCTG CGATTTACGA ACCCGCAGCC TGCATCGCCC 
TGGGATAACT TGCGAGAAGC CACCTCCTAC CCTAATTTGT GCCTCCAGAA CTCAGAGTGG 
CTGCTCTTAG ATCAACATAT GCTCAAGGTG CATTACCCGA AATTCGGAGT GTCAGAAGAC 
TGCCTCTACC TGAACATCTA TGCGCCTGCC CACGCCGATA CAGGCTCCAA GCTCCCCGTC 
TTGGTGTGGT TCCCAGGAGG TGCCTTCAAG ACTGGCTCAG CCTCCATCTT TGATGGGTCC 
GCCCTGGCTG CCTATGAGGA CGTGCTGGTT GTGGTCGTCC AGTACCGGCT AGGAATATTT 
GGTTTCTTCA CCACATGGGA TCAGCATGCT CCGGGGAACT GGGCCTTCAA GGACCAGGTG 
GCTGCTCTGT CCTGGGTCCA GAAGAACATC GAGTTCTTCG GTGGGGACCC CAGCTCTGTG 
ACCATCTTTG GCGAGTCCGC GGGAGCCATA AGTGTTTCTA GTCTTATACT GTCTCCCATG 
GCCAAAGGCT TATTCCACAA AGCCATCATG GAGAGTGGGG TGGCCATCAT CCCTTACCTG 
GAGGCCCATG ATTATGAGAA GAGTGAGGAC CTGCAGGTGG TTGCACATTT CTGTGGTAAC 
AATGCGTCAG ACTCTGAGGC CCTGCTGAGG TGCCTGAGGA CAAAACCCTC CAAGGAGCTG 
CTGACCCTCA GCCAGAAAAC AAAGTCTTTC ACTCGAGTGG TTGATGGTGC TTTCTTTCCT 
AATGAGCCTC TAGATCTATT GTCTCAGAAA GCATTTAAAG CAATTCCTTC CATCATCGGA 
GTCAATAACC ACGAGTGTGG CTTCCTGCTG CCTATGAAGG AGGCTCCTGA GATCCTCAGT 
GGCTCCAACA AGTCCCTTGC CCTCCATCTG ATACAAAACA TCCTGCACAT CCCGCCTCAG 
TATTTGCACC TTGTGGCTAA TGAATACTTC CATGACAAGC ACTCCCTGAC TGAAATCCGA 
GACAGTCTTC TGGACTTGCT TGGAGATGTG TTCTTTGTGG TCCCTGCACT GATCACAGCT 
CGATATCACA GAGAAGGAGC CACGGAGGAG GAGAAGTTAC TGAGCCGGAA GATGATGAAA 
TACTGGGCTA CCTTTGCTCG AACCGGGAAT CCTAATGGGA ACGACCTGTC TCTGTGGCCA 
GCTTATAATC TGACTGAGCA GTACCTCCAG CTGGACTTGA ACATGAGCCT CGGACAGAGA 
CTCAAAGAAC CGCGGGTGGA TTTTTGGACC AGCACCATCC CCCTGATCCT GTCTGCCTCC 
GACATGCTCC ACAGTCCTCT TTCTTCCTTA ACTTTCCTCT CTCTCCTCCA GCCTTTCTTT 
TTCTTTTGTG CTCCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_659461.1
CDS	153..1730
Translation	MSGNWVHPGQILIWAIWVLAAPTKGPSAEGPQRNTRLGWIQGKQVTVLGSPVPVNVFLGVPFAAPPLGSLRFTNPQPASPWDNLREATSYPNLCLQNSEWLLLDQHMLKVHYPKFGVSEDCLYLNIYAPAHADTGSKLPVLVWFPGGAFKTGSASIFDGSALAAYEDVLVVVVQYRLGIFGFFTTWDQHAPGNWAFKDQVAALSWVQKNIEFFGGDPSSVTIFGESAGAISVSSLILSPMAKGLFHKAIMESGVAIIPYLEAHDYEKSEDLQVVAHFCGNNASDSEALLRCLRTKPSKELLTLSQKTKSFTRVVDGAFFPNEPLDLLSQKAFKAIPSIIGVNNHECGFLLPMKEAPEILSGSNKSLALHLIQNILHIPPQYLHLVANEYFHDKHSLTEIRDSLLDLLGDVFFVVPALITARYHREGATEEEKLLSRKMMKYWATFARTGNPNGNDLSLWPAYNLTEQYLQLDLNMSLGQRLKEPRVDFWTSTIPLILSASDMLHSPLSSLTFLSLLQPFFFFCAP

Target ORF information:

RefSeq Version	NM_145024.3
Organism	Homo sapiens(human)
Definition	Homo sapiens carboxylesterase 5A (CES5A), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_145024.3

ORF Insert Sequence:

ATGAGTGGGA ATTGGGTGCA CCCAGGCCAG ATCCTAATTT GGGCTATCTG GGTCCTTGCA 
GCCCCCACCA AAGGGCCTTC TGCTGAAGGG CCACAGAGGA ACACCAGGCT GGGATGGATT 
CAGGGCAAGC AAGTCACTGT GCTGGGAAGC CCTGTGCCTG TGAACGTGTT CCTCGGAGTC 
CCCTTTGCTG CTCCCCCGCT GGGATCCCTG CGATTTACGA ACCCGCAGCC TGCATCGCCC 
TGGGATAACT TGCGAGAAGC CACCTCCTAC CCTAATTTGT GCCTCCAGAA CTCAGAGTGG 
CTGCTCTTAG ATCAACATAT GCTCAAGGTG CATTACCCGA AATTCGGAGT GTCAGAAGAC 
TGCCTCTACC TGAACATCTA TGCGCCTGCC CACGCCGATA CAGGCTCCAA GCTCCCCGTC 
TTGGTGTGGT TCCCAGGAGG TGCCTTCAAG ACTGGCTCAG CCTCCATCTT TGATGGGTCC 
GCCCTGGCTG CCTATGAGGA CGTGCTGGTT GTGGTCGTCC AGTACCGGCT AGGAATATTT 
GGTTTCTTCA CCACATGGGA TCAGCATGCT CCGGGGAACT GGGCCTTCAA GGACCAGGTG 
GCTGCTCTGT CCTGGGTCCA GAAGAACATC GAGTTCTTCG GTGGGGACCC CAGCTCTGTG 
ACCATCTTTG GCGAGTCCGC GGGAGCCATA AGTGTTTCTA GTCTTATACT GTCTCCCATG 
GCCAAAGGCT TATTCCACAA AGCCATCATG GAGAGTGGGG TGGCCATCAT CCCTTACCTG 
GAGGCCCATG ATTATGAGAA GAGTGAGGAC CTGCAGGTGG TTGCACATTT CTGTGGTAAC 
AATGCGTCAG ACTCTGAGGC CCTGCTGAGG TGCCTGAGGA CAAAACCCTC CAAGGAGCTG 
CTGACCCTCA GCCAGAAAAC AAAGTCTTTC ACTCGAGTGG TTGATGGTGC TTTCTTTCCT 
AATGAGCCTC TAGATCTATT GTCTCAGAAA GCATTTAAAG CAATTCCTTC CATCATCGGA 
GTCAATAACC ACGAGTGTGG CTTCCTGCTG CCTATGAAGG AGGCTCCTGA GATCCTCAGT 
GGCTCCAACA AGTCCCTTGC CCTCCATCTG ATACAAAACA TCCTGCACAT CCCGCCTCAG 
TATTTGCACC TTGTGGCTAA TGAATACTTC CATGACAAGC ACTCCCTGAC TGAAATCCGA 
GACAGTCTTC TGGACTTGCT TGGAGATGTG TTCTTTGTGG TCCCTGCACT GATCACAGCT 
CGATATCACA GAGAAGGAGC CACGGAGGAG GAGAAGTTAC TGAGCCGGAA GATGATGAAA 
TACTGGGCTA CCTTTGCTCG AACCGGGAAT CCTAATGGGA ACGACCTGTC TCTGTGGCCA 
GCTTATAATC TGACTGAGCA GTACCTCCAG CTGGACTTGA ACATGAGCCT CGGACAGAGA 
CTCAAAGAAC CGCGGGTGGA TTTTTGGACC AGCACCATCC CCCTGATCCT GTCTGCCTCC 
GACATGCTCC ACAGTCCTCT TTCTTCCTTA ACTTTCCTCT CTCTCCTCCA GCCTTTCTTT 
TTCTTTTGTG CTCCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CES5A ( NM_145024.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_659461.1 Homo sapiens carboxylesterase 5A (CES5A), transcript variant 2, mRNA.

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CloneID	OHu06445
Clone ID Related Accession (Same CDS sequence)	NM_145024.3 , NM_145024.2
Accession Version	NM_145024.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1578bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Homo sapiens(human)
Product	carboxylesterase 5A isoform 2 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK056109.1, AC147362.2 and BC039073.1. On Jan 3, 2009 this sequence version replaced NM_145024.1. Transcript Variant: This variant (2) has an alternate 5' sequence, and omits an in-frame exon in the 3' CDS, as compared to variant 3. The resulting isoform (2) is shorter; has a different N-terminus and lacks an internal segment, as compared to isoform 3. ##Evidence-Data-START## Transcript exon combination :: AK056109.1, BC069548.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1968968, SAMEA2144335 [ECO:0000350] ##Evidence-Data-END##

ATGAGTGGGA ATTGGGTGCA CCCAGGCCAG ATCCTAATTT GGGCTATCTG GGTCCTTGCA 
GCCCCCACCA AAGGGCCTTC TGCTGAAGGG CCACAGAGGA ACACCAGGCT GGGATGGATT 
CAGGGCAAGC AAGTCACTGT GCTGGGAAGC CCTGTGCCTG TGAACGTGTT CCTCGGAGTC 
CCCTTTGCTG CTCCCCCGCT GGGATCCCTG CGATTTACGA ACCCGCAGCC TGCATCGCCC 
TGGGATAACT TGCGAGAAGC CACCTCCTAC CCTAATTTGT GCCTCCAGAA CTCAGAGTGG 
CTGCTCTTAG ATCAACATAT GCTCAAGGTG CATTACCCGA AATTCGGAGT GTCAGAAGAC 
TGCCTCTACC TGAACATCTA TGCGCCTGCC CACGCCGATA CAGGCTCCAA GCTCCCCGTC 
TTGGTGTGGT TCCCAGGAGG TGCCTTCAAG ACTGGCTCAG CCTCCATCTT TGATGGGTCC 
GCCCTGGCTG CCTATGAGGA CGTGCTGGTT GTGGTCGTCC AGTACCGGCT AGGAATATTT 
GGTTTCTTCA CCACATGGGA TCAGCATGCT CCGGGGAACT GGGCCTTCAA GGACCAGGTG 
GCTGCTCTGT CCTGGGTCCA GAAGAACATC GAGTTCTTCG GTGGGGACCC CAGCTCTGTG 
ACCATCTTTG GCGAGTCCGC GGGAGCCATA AGTGTTTCTA GTCTTATACT GTCTCCCATG 
GCCAAAGGCT TATTCCACAA AGCCATCATG GAGAGTGGGG TGGCCATCAT CCCTTACCTG 
GAGGCCCATG ATTATGAGAA GAGTGAGGAC CTGCAGGTGG TTGCACATTT CTGTGGTAAC 
AATGCGTCAG ACTCTGAGGC CCTGCTGAGG TGCCTGAGGA CAAAACCCTC CAAGGAGCTG 
CTGACCCTCA GCCAGAAAAC AAAGTCTTTC ACTCGAGTGG TTGATGGTGC TTTCTTTCCT 
AATGAGCCTC TAGATCTATT GTCTCAGAAA GCATTTAAAG CAATTCCTTC CATCATCGGA 
GTCAATAACC ACGAGTGTGG CTTCCTGCTG CCTATGAAGG AGGCTCCTGA GATCCTCAGT 
GGCTCCAACA AGTCCCTTGC CCTCCATCTG ATACAAAACA TCCTGCACAT CCCGCCTCAG 
TATTTGCACC TTGTGGCTAA TGAATACTTC CATGACAAGC ACTCCCTGAC TGAAATCCGA 
GACAGTCTTC TGGACTTGCT TGGAGATGTG TTCTTTGTGG TCCCTGCACT GATCACAGCT 
CGATATCACA GAGAAGGAGC CACGGAGGAG GAGAAGTTAC TGAGCCGGAA GATGATGAAA 
TACTGGGCTA CCTTTGCTCG AACCGGGAAT CCTAATGGGA ACGACCTGTC TCTGTGGCCA 
GCTTATAATC TGACTGAGCA GTACCTCCAG CTGGACTTGA ACATGAGCCT CGGACAGAGA 
CTCAAAGAAC CGCGGGTGGA TTTTTGGACC AGCACCATCC CCCTGATCCT GTCTGCCTCC 
GACATGCTCC ACAGTCCTCT TTCTTCCTTA ACTTTCCTCT CTCTCCTCCA GCCTTTCTTT 
TTCTTTTGTG CTCCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_659461.1
CDS	150..1727
Translation	MSGNWVHPGQILIWAIWVLAAPTKGPSAEGPQRNTRLGWIQGKQVTVLGSPVPVNVFLGVPFAAPPLGSLRFTNPQPASPWDNLREATSYPNLCLQNSEWLLLDQHMLKVHYPKFGVSEDCLYLNIYAPAHADTGSKLPVLVWFPGGAFKTGSASIFDGSALAAYEDVLVVVVQYRLGIFGFFTTWDQHAPGNWAFKDQVAALSWVQKNIEFFGGDPSSVTIFGESAGAISVSSLILSPMAKGLFHKAIMESGVAIIPYLEAHDYEKSEDLQVVAHFCGNNASDSEALLRCLRTKPSKELLTLSQKTKSFTRVVDGAFFPNEPLDLLSQKAFKAIPSIIGVNNHECGFLLPMKEAPEILSGSNKSLALHLIQNILHIPPQYLHLVANEYFHDKHSLTEIRDSLLDLLGDVFFVVPALITARYHREGATEEEKLLSRKMMKYWATFARTGNPNGNDLSLWPAYNLTEQYLQLDLNMSLGQRLKEPRVDFWTSTIPLILSASDMLHSPLSSLTFLSLLQPFFFFCAP

Target ORF information:

RefSeq Version	NM_145024.2
Organism	Homo sapiens(human)
Definition	Homo sapiens carboxylesterase 5A (CES5A), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_145024.2

ORF Insert Sequence:

ATGAGTGGGA ATTGGGTGCA CCCAGGCCAG ATCCTAATTT GGGCTATCTG GGTCCTTGCA 
GCCCCCACCA AAGGGCCTTC TGCTGAAGGG CCACAGAGGA ACACCAGGCT GGGATGGATT 
CAGGGCAAGC AAGTCACTGT GCTGGGAAGC CCTGTGCCTG TGAACGTGTT CCTCGGAGTC 
CCCTTTGCTG CTCCCCCGCT GGGATCCCTG CGATTTACGA ACCCGCAGCC TGCATCGCCC 
TGGGATAACT TGCGAGAAGC CACCTCCTAC CCTAATTTGT GCCTCCAGAA CTCAGAGTGG 
CTGCTCTTAG ATCAACATAT GCTCAAGGTG CATTACCCGA AATTCGGAGT GTCAGAAGAC 
TGCCTCTACC TGAACATCTA TGCGCCTGCC CACGCCGATA CAGGCTCCAA GCTCCCCGTC 
TTGGTGTGGT TCCCAGGAGG TGCCTTCAAG ACTGGCTCAG CCTCCATCTT TGATGGGTCC 
GCCCTGGCTG CCTATGAGGA CGTGCTGGTT GTGGTCGTCC AGTACCGGCT AGGAATATTT 
GGTTTCTTCA CCACATGGGA TCAGCATGCT CCGGGGAACT GGGCCTTCAA GGACCAGGTG 
GCTGCTCTGT CCTGGGTCCA GAAGAACATC GAGTTCTTCG GTGGGGACCC CAGCTCTGTG 
ACCATCTTTG GCGAGTCCGC GGGAGCCATA AGTGTTTCTA GTCTTATACT GTCTCCCATG 
GCCAAAGGCT TATTCCACAA AGCCATCATG GAGAGTGGGG TGGCCATCAT CCCTTACCTG 
GAGGCCCATG ATTATGAGAA GAGTGAGGAC CTGCAGGTGG TTGCACATTT CTGTGGTAAC 
AATGCGTCAG ACTCTGAGGC CCTGCTGAGG TGCCTGAGGA CAAAACCCTC CAAGGAGCTG 
CTGACCCTCA GCCAGAAAAC AAAGTCTTTC ACTCGAGTGG TTGATGGTGC TTTCTTTCCT 
AATGAGCCTC TAGATCTATT GTCTCAGAAA GCATTTAAAG CAATTCCTTC CATCATCGGA 
GTCAATAACC ACGAGTGTGG CTTCCTGCTG CCTATGAAGG AGGCTCCTGA GATCCTCAGT 
GGCTCCAACA AGTCCCTTGC CCTCCATCTG ATACAAAACA TCCTGCACAT CCCGCCTCAG 
TATTTGCACC TTGTGGCTAA TGAATACTTC CATGACAAGC ACTCCCTGAC TGAAATCCGA 
GACAGTCTTC TGGACTTGCT TGGAGATGTG TTCTTTGTGG TCCCTGCACT GATCACAGCT 
CGATATCACA GAGAAGGAGC CACGGAGGAG GAGAAGTTAC TGAGCCGGAA GATGATGAAA 
TACTGGGCTA CCTTTGCTCG AACCGGGAAT CCTAATGGGA ACGACCTGTC TCTGTGGCCA 
GCTTATAATC TGACTGAGCA GTACCTCCAG CTGGACTTGA ACATGAGCCT CGGACAGAGA 
CTCAAAGAAC CGCGGGTGGA TTTTTGGACC AGCACCATCC CCCTGATCCT GTCTGCCTCC 
GACATGCTCC ACAGTCCTCT TTCTTCCTTA ACTTTCCTCT CTCTCCTCCA GCCTTTCTTT 
TTCTTTTGTG CTCCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome wide association study (GWAS) of Chagas cardiomyopathy in Trypanosoma cruzi seropositive subjects.
PloS one8(11)e79629(2013)
Deng X,Sabino EC,Cunha-Neto E,Ribeiro AL,Ianni B,Mady C,Busch MP,Seielstad M,

Recommended nomenclature for five mammalian carboxylesterase gene families: human, mouse, and rat genes and proteins.
Mammalian genome : official journal of the International Mammalian Genome Society21(9-10)427-41(2010 Oct)
Holmes RS,Wright MW,Laulederkind SJ,Cox LA,Hosokawa M,Imai T,Ishibashi S,Lehner R,Miyazaki M,Perkins EJ,Potter PM,Redinbo MR,Robert J,Satoh T,Yamashita T,Yan B,Yokoi T,Zechner R,Maltais LJ

Mammalian carboxylesterase 3: comparative genomics and proteomics.
Genetica138(7)695-708(2010 Jul)
Holmes RS,Cox LA,VandeBerg JL

A new class of mammalian carboxylesterase CES6.
Comparative biochemistry and physiology. Part D, Genomics & proteomics4(3)209-17(2009 Sep)
Holmes RS,Cox LA,Vandeberg JL

Mammalian carboxylesterase 5: comparative biochemistry and genomics.
Comparative biochemistry and physiology. Part D, Genomics & proteomics3(3)195-204(2008 Sep)
Holmes RS,Cox LA,Vandeberg JL

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CES5A cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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