FARSA cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	FARSA
Entrez Gene ID	2193
Full Name	phenylalanyl-tRNA synthetase alpha subunit
Synonyms	CML33,FARSL,FARSLA,FRSA,PheHA
General protein information	Preferred Names phenylalanyl-tRNA synthetase alpha subunit Names phenylalanine--tRNA ligase alpha subunit pheRS phenylalanine tRNA ligase 1, alpha, cytoplasmic phenylalanine--tRNA ligase alpha chain phenylalanine-tRNA synthetase alpha-subunit phenylalanine-tRNA synthetase-like, alpha subunit phenylalanyl-tRNA synthetase alpha chain phenylalanyl-tRNA synthetase-like, alpha subunit
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 19 Map Location: 19p13.13
Summary	Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. This gene encodes a product which is similar to the catalytic subunit of prokaryotic and Saccharomyces cerevisiae phenylalanyl-tRNA synthetases (PheRS). This gene product has been shown to be expressed in a tumor-selective and cell cycle stage- and differentiation-dependent manner, the first member of the tRNA synthetase gene family shown to exhibit this type of regulated expression [provided by RefSeq, Jul 2008].
Disorder MIM:	602918

mRNA and Protein(s)

mRNA	Protein	Name
NM_004461.2	NP_004452.1	phenylalanine--tRNA ligase alpha subunit
NM_004461.3	NP_004452.1	phenylalanine--tRNA ligase alpha subunit
XM_024451419.1	XP_024307187.1	phenylalanine--tRNA ligase alpha subunit isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	FARSA	NP_004452.1
Pan troglodytes (chimpanzee)	FARSA	NP_001233393.1
	FARSA	XP_001110025.1
Danio rerio (zebrafish)	farsa	NP_001038760.2
Caenorhabditis elegans (roundworm)	fars-1	NP_491792.1
Saccharomyces cerevisiae (baker's yeast)	FRS2	NP_116631.1
Gallus gallus (chicken)	FARSA	NP_001258086.1
Canis lupus familiaris (dog)	FARSA	XP_533900.1
Bos taurus (cattle)	FARSA	NP_001094577.1
Rattus norvegicus (Norway rat)	Farsa	NP_001019408.1
Drosophila melanogaster (fruit fly)	CG2263	NP_572448.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002653	XP_312272.1
Arabidopsis thaliana (thale cress)	AT4G39280	NP_195639.2
Kluyveromyces lactis	KLLA0F25234g	XP_456205.1
Eremothecium gossypii	AGOS_AFR516W	NP_986063.1
Schizosaccharomyces pombe (fission yeast)	frs2	NP_594078.1
Oryza sativa (rice)	Os10g0400800	NP_001064545.1
Xenopus tropicalis (tropical clawed frog)	farsa	NP_001072827.1
Mus musculus (house mouse)	Farsa	NP_079924.2
Magnaporthe oryzae (rice blast fungus)	MGG_04425	XP_003713518.1
Neurospora crassa	NCU05095	XP_957081.1

Gene Ontology
Bibliography

Related articles in PubMed

Architecture of the human interactome defines protein communities and disease networks.
Huttlin EL, Bruckner RJ, Paulo JA, Cannon JR, Ting L, Baltier K, Colby G, Gebreab F, Gygi MP, Parzen H, Szpyt J, Tam S, Zarraga G, Pontano-Vaites L, Swarup S, White AE, Schweppe DK, Rad R, Erickson BK, Obar RA, Guruharsha KG, Li K, Artavanis-Tsakonas S, Gygi SP, Harper JW
Nature545(7655)505-509(2017 05)

Host factors that interact with the pestivirus N-terminal protease, Npro, are components of the ribonucleoprotein complex.
Jefferson M, Donaszi-Ivanov A, Pollen S, Dalmay T, Saalbach G, Powell PP
Journal of virology88(18)10340-53(2014 Sep)

Clozapine-induced agranulocytosis is associated with rare HLA-DQB1 and HLA-B alleles.
Goldstein JI, Jarskog LF, Hilliard C, Alfirevic A, Duncan L, Fourches D, Huang H, Lek M, Neale BM, Ripke S, Shianna K, Szatkiewicz JP, Tropsha A, van den Oord EJ, Cascorbi I, Dettling M, Gazit E, Goff DC, Holden AL, Kelly DL, Malhotra AK, Nielsen J, Pirmohamed M, Rujescu D, Werge T, Levy DL, Josiassen RC, Kennedy JL, Lieberman JA, Daly MJ, Sullivan PF
Nature communications54757(2014 Sep)

Genome wide association study (GWAS) of Chagas cardiomyopathy in Trypanosoma cruzi seropositive subjects.
Deng X, Sabino EC, Cunha-Neto E, Ribeiro AL, Ianni B, Mady C, Busch MP, Seielstad M,
PloS one8(11)e79629(2013)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Using shotgun mass spectrometry, we found this protein differentially expressed in the dorsolateral prefrontal cortex from patients with schizophrenia.
Title: Prefrontal cortex shotgun proteome analysis reveals altered calcium homeostasis and immune system imbalance in schizophrenia.

The following FARSA gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the FARSA cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu04363	NM_004461.3 Latest version!	Homo sapiens phenylalanyl-tRNA synthetase subunit alpha (FARSA), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$219.50-$307.30 $439.00
OHu104643	XM_024451419.1 Latest version!	Homo sapiens phenylalanyl-tRNA synthetase alpha subunit (FARSA), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
View Old Accession Versions >>
OHu04363	NM_004461.2	Homo sapiens phenylalanyl-tRNA synthetase alpha subunit (FARSA), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$219.50-$307.30 $439.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

FARSA ( NM_004461.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_004452.1 Homo sapiens phenylalanyl-tRNA synthetase alpha subunit (FARSA), mRNA.

Price & Availability↑

CloneID	OHu04363
Clone ID Related Accession (Same CDS sequence)	NM_004461.2 , NM_004461.3
Accession Version	NM_004461.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1527bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-26
Organism	Homo sapiens(human)
Product	phenylalanine--tRNA ligase alpha subunit
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC092069.2, AK130195.1, U07424.1, BC006495.2 and BG284015.1. On Mar 2, 2007 this sequence version replaced NM_004461.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.89387.1, SRR1803616.100859.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGATG GTCAGGTGGC GGAACTGCTG CTCCGGCGGC TGGAGGCGTC TGATGGCGGC 
CTGGACAGCG CCGAGTTGGC GGCTGAGCTG GGCATGGAGC ACCAGGCGGT GGTGGGCGCC 
GTGAAGAGCC TTCAGGCGCT GGGCGAGGTC ATCGAGGCTG AACTTCGGTC CACCAAGCAC 
TGGGAGCTTA CTGCGGAGGG CGAGGAGATT GCCCGGGAGG GCAGCCATGA GGCCCGTGTG 
TTTCGAAGCA TTCCCCCAGA GGGCCTGGCC CAGAGCGAGC TTATGCGACT GCCCAGTGGC 
AAAGTGGGCT TCAGCAAGGC CATGTCCAAC AAGTGGATTC GGGTGGACAA GAGTGCGGCT 
GACGGGCCCC GGGTGTTCCG AGTGGTGGAC AGCATGGAGG ATGAGGTGCA GCGGCGGCTC 
CAGCTGGTCC GGGGGGGACA GGCTGAGAAG CTGGGGGAGA AGGAGAGGAG CGAGCTGAGG 
AAGAGGAAGC TGTTGGCTGA AGTGACTCTG AAGACCTACT GGGTGAGCAA AGGCAGTGCC 
TTTAGTACCA GCATCTCCAA GCAAGAGACA GAGCTGAGCC CAGAGATGAT CTCCAGTGGC 
TCTTGGCGGG ACCGGCCCTT CAAGCCCTAC AACTTCTTGG CCCACGGTGT CCTCCCCGAC 
AGCGGCCACC TTCACCCGCT GCTCAAGGTC CGCTCCCAGT TCCGACAGAT CTTCCTGGAG 
ATGGGGTTCA CCGAGATGCC GACTGATAAC TTCATTGAGA GCTCCTTCTG GAACTTTGAC 
GCCCTCTTCC AGCCCCAGCA GCACCCAGCC CGTGACCAGC ACGACACCTT CTTCCTTCGA 
GATCCAGCGG AGGCCCTGCA GCTCCCAATG GACTATGTCC AGCGGGTCAA GCGGACCCAC 
TCTCAGGGCG GCTACGGCTC ACAGGGGTAC AAGTATAACT GGAAGCTGGA CGAGGCCCGG 
AAAAACCTAC TGCGAACCCA CACCACATCA GCCAGCGCCC GTGCGCTCTA CCGCCTTGCC 
CAGAAGAAGC CCTTCACTCC GGTCAAGTAC TTCTCCATCG ACCGCGTATT CCGGAATGAG 
ACCCTGGACG CCACGCACCT GGCTGAGTTC CACCAGATCG AGGGCGTGGT GGCGGATCAT 
GGTCTCACCT TGGGCCACCT CATGGGCGTT CTGCGGGAGT TCTTCACCAA GCTGGGTATC 
ACGCAACTCC GCTTCAAGCC AGCCTACAAC CCATACACAG AGCCCAGCAT GGAGGTGTTC 
AGCTACCACC AAGGCCTGAA GAAGTGGGTG GAGGTCGGAA ACTCGGGGGT CTTCCGTCCA 
GAGATGCTGC TGCCCATGGG GCTTCCCGAG AACGTGTCGG TCATTGCCTG GGGCCTCTCC 
CTGGAGCGCC CAACGATGAT CAAATATGGC ATCAACAATA TCCGGGAGCT GGTGGGCCAC 
AAGGTGAACC TGCAGATGGT GTATGACAGT CCCCTGTGCC GCCTGGATGC CGAGCCGAGG 
CCCCCTCCCA CACAGGAGGC TGCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004452.1
CDS	49..1575
Translation	MADGQVAELLLRRLEASDGGLDSAELAAELGMEHQAVVGAVKSLQALGEVIEAELRSTKHWELTAEGEEIAREGSHEARVFRSIPPEGLAQSELMRLPSGKVGFSKAMSNKWIRVDKSAADGPRVFRVVDSMEDEVQRRLQLVRGGQAEKLGEKERSELRKRKLLAEVTLKTYWVSKGSAFSTSISKQETELSPEMISSGSWRDRPFKPYNFLAHGVLPDSGHLHPLLKVRSQFRQIFLEMGFTEMPTDNFIESSFWNFDALFQPQQHPARDQHDTFFLRDPAEALQLPMDYVQRVKRTHSQGGYGSQGYKYNWKLDEARKNLLRTHTTSASARALYRLAQKKPFTPVKYFSIDRVFRNETLDATHLAEFHQIEGVVADHGLTLGHLMGVLREFFTKLGITQLRFKPAYNPYTEPSMEVFSYHQGLKKWVEVGNSGVFRPEMLLPMGLPENVSVIAWGLSLERPTMIKYGINNIRELVGHKVNLQMVYDSPLCRLDAEPRPPPTQEAA

Target ORF information:

RefSeq Version	NM_004461.2
Organism	Homo sapiens(human)
Definition	Homo sapiens phenylalanyl-tRNA synthetase alpha subunit (FARSA), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004461.2

ORF Insert Sequence:

ATGGCGGATG GTCAGGTGGC GGAACTGCTG CTCCGGCGGC TGGAGGCGTC TGATGGCGGC 
CTGGACAGCG CCGAGTTGGC GGCTGAGCTG GGCATGGAGC ACCAGGCGGT GGTGGGCGCC 
GTGAAGAGCC TTCAGGCGCT GGGCGAGGTC ATCGAGGCTG AACTTCGGTC CACCAAGCAC 
TGGGAGCTTA CTGCGGAGGG CGAGGAGATT GCCCGGGAGG GCAGCCATGA GGCCCGTGTG 
TTTCGAAGCA TTCCCCCAGA GGGCCTGGCC CAGAGCGAGC TTATGCGACT GCCCAGTGGC 
AAAGTGGGCT TCAGCAAGGC CATGTCCAAC AAGTGGATTC GGGTGGACAA GAGTGCGGCT 
GACGGGCCCC GGGTGTTCCG AGTGGTGGAC AGCATGGAGG ATGAGGTGCA GCGGCGGCTC 
CAGCTGGTCC GGGGGGGACA GGCTGAGAAG CTGGGGGAGA AGGAGAGGAG CGAGCTGAGG 
AAGAGGAAGC TGTTGGCTGA AGTGACTCTG AAGACCTACT GGGTGAGCAA AGGCAGTGCC 
TTTAGTACCA GCATCTCCAA GCAAGAGACA GAGCTGAGCC CAGAGATGAT CTCCAGTGGC 
TCTTGGCGGG ACCGGCCCTT CAAGCCCTAC AACTTCTTGG CCCACGGTGT CCTCCCCGAC 
AGCGGCCACC TTCACCCGCT GCTCAAGGTC CGCTCCCAGT TCCGACAGAT CTTCCTGGAG 
ATGGGGTTCA CCGAGATGCC GACTGATAAC TTCATTGAGA GCTCCTTCTG GAACTTTGAC 
GCCCTCTTCC AGCCCCAGCA GCACCCAGCC CGTGACCAGC ACGACACCTT CTTCCTTCGA 
GATCCAGCGG AGGCCCTGCA GCTCCCAATG GACTATGTCC AGCGGGTCAA GCGGACCCAC 
TCTCAGGGCG GCTACGGCTC ACAGGGGTAC AAGTATAACT GGAAGCTGGA CGAGGCCCGG 
AAAAACCTAC TGCGAACCCA CACCACATCA GCCAGCGCCC GTGCGCTCTA CCGCCTTGCC 
CAGAAGAAGC CCTTCACTCC GGTCAAGTAC TTCTCCATCG ACCGCGTATT CCGGAATGAG 
ACCCTGGACG CCACGCACCT GGCTGAGTTC CACCAGATCG AGGGCGTGGT GGCGGATCAT 
GGTCTCACCT TGGGCCACCT CATGGGCGTT CTGCGGGAGT TCTTCACCAA GCTGGGTATC 
ACGCAACTCC GCTTCAAGCC AGCCTACAAC CCATACACAG AGCCCAGCAT GGAGGTGTTC 
AGCTACCACC AAGGCCTGAA GAAGTGGGTG GAGGTCGGAA ACTCGGGGGT CTTCCGTCCA 
GAGATGCTGC TGCCCATGGG GCTTCCCGAG AACGTGTCGG TCATTGCCTG GGGCCTCTCC 
CTGGAGCGCC CAACGATGAT CAAATATGGC ATCAACAATA TCCGGGAGCT GGTGGGCCAC 
AAGGTGAACC TGCAGATGGT GTATGACAGT CCCCTGTGCC GCCTGGATGC CGAGCCGAGG 
CCCCCTCCCA CACAGGAGGC TGCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FARSA ( NM_004461.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_004452.1 Homo sapiens phenylalanyl-tRNA synthetase subunit alpha (FARSA), mRNA.

Price & Availability↑

CloneID	OHu04363
Clone ID Related Accession (Same CDS sequence)	NM_004461.2 , NM_004461.3
Accession Version	NM_004461.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1527bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-10
Organism	Homo sapiens(human)
Product	phenylalanine--tRNA ligase alpha subunit
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK130195.1, U07424.1 and BC006495.2. On Nov 22, 2018 this sequence version replaced NM_004461.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR3476690.129187.1, SRR3476690.248516.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000314606.9/ ENSP00000320309.3 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCGGATG GTCAGGTGGC GGAACTGCTG CTCCGGCGGC TGGAGGCGTC TGATGGCGGC 
CTGGACAGCG CCGAGTTGGC GGCTGAGCTG GGCATGGAGC ACCAGGCGGT GGTGGGCGCC 
GTGAAGAGCC TTCAGGCGCT GGGCGAGGTC ATCGAGGCTG AACTTCGGTC CACCAAGCAC 
TGGGAGCTTA CTGCGGAGGG CGAGGAGATT GCCCGGGAGG GCAGCCATGA GGCCCGTGTG 
TTTCGAAGCA TTCCCCCAGA GGGCCTGGCC CAGAGCGAGC TTATGCGACT GCCCAGTGGC 
AAAGTGGGCT TCAGCAAGGC CATGTCCAAC AAGTGGATTC GGGTGGACAA GAGTGCGGCT 
GACGGGCCCC GGGTGTTCCG AGTGGTGGAC AGCATGGAGG ATGAGGTGCA GCGGCGGCTC 
CAGCTGGTCC GGGGGGGACA GGCTGAGAAG CTGGGGGAGA AGGAGAGGAG CGAGCTGAGG 
AAGAGGAAGC TGTTGGCTGA AGTGACTCTG AAGACCTACT GGGTGAGCAA AGGCAGTGCC 
TTTAGTACCA GCATCTCCAA GCAAGAGACA GAGCTGAGCC CAGAGATGAT CTCCAGTGGC 
TCTTGGCGGG ACCGGCCCTT CAAGCCCTAC AACTTCTTGG CCCACGGTGT CCTCCCCGAC 
AGCGGCCACC TTCACCCGCT GCTCAAGGTC CGCTCCCAGT TCCGACAGAT CTTCCTGGAG 
ATGGGGTTCA CCGAGATGCC GACTGATAAC TTCATTGAGA GCTCCTTCTG GAACTTTGAC 
GCCCTCTTCC AGCCCCAGCA GCACCCAGCC CGTGACCAGC ACGACACCTT CTTCCTTCGA 
GATCCAGCGG AGGCCCTGCA GCTCCCAATG GACTATGTCC AGCGGGTCAA GCGGACCCAC 
TCTCAGGGCG GCTACGGCTC ACAGGGGTAC AAGTATAACT GGAAGCTGGA CGAGGCCCGG 
AAAAACCTAC TGCGAACCCA CACCACATCA GCCAGCGCCC GTGCGCTCTA CCGCCTTGCC 
CAGAAGAAGC CCTTCACTCC GGTCAAGTAC TTCTCCATCG ACCGCGTATT CCGGAATGAG 
ACCCTGGACG CCACGCACCT GGCTGAGTTC CACCAGATCG AGGGCGTGGT GGCGGATCAT 
GGTCTCACCT TGGGCCACCT CATGGGCGTT CTGCGGGAGT TCTTCACCAA GCTGGGTATC 
ACGCAACTCC GCTTCAAGCC AGCCTACAAC CCATACACAG AGCCCAGCAT GGAGGTGTTC 
AGCTACCACC AAGGCCTGAA GAAGTGGGTG GAGGTCGGAA ACTCGGGGGT CTTCCGTCCA 
GAGATGCTGC TGCCCATGGG GCTTCCCGAG AACGTGTCGG TCATTGCCTG GGGCCTCTCC 
CTGGAGCGCC CAACGATGAT CAAATATGGC ATCAACAATA TCCGGGAGCT GGTGGGCCAC 
AAGGTGAACC TGCAGATGGT GTATGACAGT CCCCTGTGCC GCCTGGATGC CGAGCCGAGG 
CCCCCTCCCA CACAGGAGGC TGCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004452.1
CDS	16..1542
Translation	MADGQVAELLLRRLEASDGGLDSAELAAELGMEHQAVVGAVKSLQALGEVIEAELRSTKHWELTAEGEEIAREGSHEARVFRSIPPEGLAQSELMRLPSGKVGFSKAMSNKWIRVDKSAADGPRVFRVVDSMEDEVQRRLQLVRGGQAEKLGEKERSELRKRKLLAEVTLKTYWVSKGSAFSTSISKQETELSPEMISSGSWRDRPFKPYNFLAHGVLPDSGHLHPLLKVRSQFRQIFLEMGFTEMPTDNFIESSFWNFDALFQPQQHPARDQHDTFFLRDPAEALQLPMDYVQRVKRTHSQGGYGSQGYKYNWKLDEARKNLLRTHTTSASARALYRLAQKKPFTPVKYFSIDRVFRNETLDATHLAEFHQIEGVVADHGLTLGHLMGVLREFFTKLGITQLRFKPAYNPYTEPSMEVFSYHQGLKKWVEVGNSGVFRPEMLLPMGLPENVSVIAWGLSLERPTMIKYGINNIRELVGHKVNLQMVYDSPLCRLDAEPRPPPTQEAA

Target ORF information:

RefSeq Version	NM_004461.3
Organism	Homo sapiens(human)
Definition	Homo sapiens phenylalanyl-tRNA synthetase subunit alpha (FARSA), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004461.3

ORF Insert Sequence:

ATGGCGGATG GTCAGGTGGC GGAACTGCTG CTCCGGCGGC TGGAGGCGTC TGATGGCGGC 
CTGGACAGCG CCGAGTTGGC GGCTGAGCTG GGCATGGAGC ACCAGGCGGT GGTGGGCGCC 
GTGAAGAGCC TTCAGGCGCT GGGCGAGGTC ATCGAGGCTG AACTTCGGTC CACCAAGCAC 
TGGGAGCTTA CTGCGGAGGG CGAGGAGATT GCCCGGGAGG GCAGCCATGA GGCCCGTGTG 
TTTCGAAGCA TTCCCCCAGA GGGCCTGGCC CAGAGCGAGC TTATGCGACT GCCCAGTGGC 
AAAGTGGGCT TCAGCAAGGC CATGTCCAAC AAGTGGATTC GGGTGGACAA GAGTGCGGCT 
GACGGGCCCC GGGTGTTCCG AGTGGTGGAC AGCATGGAGG ATGAGGTGCA GCGGCGGCTC 
CAGCTGGTCC GGGGGGGACA GGCTGAGAAG CTGGGGGAGA AGGAGAGGAG CGAGCTGAGG 
AAGAGGAAGC TGTTGGCTGA AGTGACTCTG AAGACCTACT GGGTGAGCAA AGGCAGTGCC 
TTTAGTACCA GCATCTCCAA GCAAGAGACA GAGCTGAGCC CAGAGATGAT CTCCAGTGGC 
TCTTGGCGGG ACCGGCCCTT CAAGCCCTAC AACTTCTTGG CCCACGGTGT CCTCCCCGAC 
AGCGGCCACC TTCACCCGCT GCTCAAGGTC CGCTCCCAGT TCCGACAGAT CTTCCTGGAG 
ATGGGGTTCA CCGAGATGCC GACTGATAAC TTCATTGAGA GCTCCTTCTG GAACTTTGAC 
GCCCTCTTCC AGCCCCAGCA GCACCCAGCC CGTGACCAGC ACGACACCTT CTTCCTTCGA 
GATCCAGCGG AGGCCCTGCA GCTCCCAATG GACTATGTCC AGCGGGTCAA GCGGACCCAC 
TCTCAGGGCG GCTACGGCTC ACAGGGGTAC AAGTATAACT GGAAGCTGGA CGAGGCCCGG 
AAAAACCTAC TGCGAACCCA CACCACATCA GCCAGCGCCC GTGCGCTCTA CCGCCTTGCC 
CAGAAGAAGC CCTTCACTCC GGTCAAGTAC TTCTCCATCG ACCGCGTATT CCGGAATGAG 
ACCCTGGACG CCACGCACCT GGCTGAGTTC CACCAGATCG AGGGCGTGGT GGCGGATCAT 
GGTCTCACCT TGGGCCACCT CATGGGCGTT CTGCGGGAGT TCTTCACCAA GCTGGGTATC 
ACGCAACTCC GCTTCAAGCC AGCCTACAAC CCATACACAG AGCCCAGCAT GGAGGTGTTC 
AGCTACCACC AAGGCCTGAA GAAGTGGGTG GAGGTCGGAA ACTCGGGGGT CTTCCGTCCA 
GAGATGCTGC TGCCCATGGG GCTTCCCGAG AACGTGTCGG TCATTGCCTG GGGCCTCTCC 
CTGGAGCGCC CAACGATGAT CAAATATGGC ATCAACAATA TCCGGGAGCT GGTGGGCCAC 
AAGGTGAACC TGCAGATGGT GTATGACAGT CCCCTGTGCC GCCTGGATGC CGAGCCGAGG 
CCCCCTCCCA CACAGGAGGC TGCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FARSA ( XM_024451419.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024307187.1 Homo sapiens phenylalanyl-tRNA synthetase alpha subunit (FARSA), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu104643
Clone ID Related Accession (Same CDS sequence)	XM_024451419.1
Accession Version	XM_024451419.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1710bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-25
Organism	Homo sapiens(human)
Product	phenylalanine--tRNA ligase alpha subunit isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Homo sapiens Annotation Release 109 Annotation Version :: 109 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGATG GTCAGGTGGC GGAACTGCTG CTCCGGCGGC TGGAGGCGTC TGATGGCGGC 
CTGGACAGCG CCGAGTTGGC GGCTGAGCTG GGCATGGAGC ACCAGGCGGT GGTGGGCGCC 
GTGAAGAGCC TTCAGGCGCT GGGCGAGGTC ATCGAGGCTG AACTTCGGTC CACCAAGCAC 
TGGGAGCTTA CTGCGGAGGG CGAGGAGATT GCCCGGGAGG GCAGCCATGA GGCCCGTGTG 
TTTCGAAGCA TTCCCCCAGA GGGCCTGGCC CAGAGCGAGC TTATGCGACT GCCCAGTGGC 
AAAGTGGGCT TCAGCAAGGC CATGTCCAAC AAGTGGATTC GGGTGGACAA GAGTGCGGCT 
GACGGGCCCC GGGTGTTCCG AGTGGTGGAC AGCATGGAGG ATGAGGTGCA GCGGCGGCTC 
CAGCTGGTCC GGGGGGGACA GGCTGAGAAG CTGGGGGAGA AGGAGAGGAG CGAGCTGAGG 
AAGAGGAAGC TGTTGGCTGA AGTGACTCTG AAGACCTACT GGGTGAGCAA AGGCAGTGCC 
TTTAGTACCA GCATCTCCAA GCAAGAGACA GAGCTGAGCC CAGAGATGAT CTCCAGTGGC 
TCTTGGCGGG ACCGGCCCTT CAAGCCCTAC AACTTCTTGG CCCACGGTGT CCTCCCCGAC 
AGCGGCCACC TTCACCCGCT GCTCAAGGTC CGCTCCCAGT TCCGACAGAT CTTCCTGGAG 
ATGGGGTTCA CCGAGATGCC GACTGATAAC TTCATTGAGA GCTCCTTCTG GAACTTTGAC 
GCCCTCTTCC AGCCCCAGCA GCACCCAGCC CGTGACCAGC ACGACACCTT CTTCCTTCGA 
GATCCAGCGG AGGCCCTGCA GCTCCCAATG GACTATGTCC AGCGGGTCAA GCGGACCCAC 
TCTCAGGGCG GCTACGGCTC ACAGGGGTAC AAGTATAACT GGAAGCTGGA CGAGGCCCGG 
AAAAACCTAC TGCGAACCCA CACCACATCA GCCAGCGCCC GTGCGCTCTA CCGCCTTGCC 
CAGAAGAAGC CCTTCACTCC GGTCAAGTAC TTCTCCATCG ACCGCGTATT CCGGAATGAG 
ACCCTGGACG CCACGCACCT GGCTGAGTTC CACCAGATCG AGGGCGTGGT GGCGGATCAT 
GGTCTCACCT TGGGCCACCT CATGGGCGTT CTGCGGGAGT TCTTCACCAA GCTGGGTATC 
ACGCAACTCC GCTTCAAGCC AGCCTACAAC CCATACACAG AGCCCAGCAT GGAGGTGTTC 
AGCTACCACC AAGGTCAGGG TAGGCCCTGG TACTGGGGTG GGAGGGAGGT CTCAGGCTAC 
TATGGTTTGG CCTGGGTCCC CCCATTGCCA TGCCTGTACC CCACCTCCCA AGCTCACCAC 
CTAGACCCAG AACCTGTACC AGCAGTGCCC AGAAACTCAA CACGCACTGC CCGTCTTTTC 
TGTCCTGCCT CTGCAGGCCT GAAGAAGTGG GTGGAGGTCG GAAACTCGGG GGTCTTCCGT 
CCAGAGATGC TGCTGCCCAT GGGGCTTCCC GAGAACGTGT CGGTCATTGC CTGGGGCCTC 
TCCCTGGAGC GCCCAACGAT GATCAAATAT GGCATCAACA ATATCCGGGA GCTGGTGGGC 
CACAAGGTGA ACCTGCAGAT GGTGTATGAC AGTCCCCTGT GCCGCCTGGA TGCCGAGCCG 
AGGCCCCCTC CCACACAGGA GGCTGCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024307187.1
CDS	20..1729
Translation	MADGQVAELLLRRLEASDGGLDSAELAAELGMEHQAVVGAVKSLQALGEVIEAELRSTKHWELTAEGEEIAREGSHEARVFRSIPPEGLAQSELMRLPSGKVGFSKAMSNKWIRVDKSAADGPRVFRVVDSMEDEVQRRLQLVRGGQAEKLGEKERSELRKRKLLAEVTLKTYWVSKGSAFSTSISKQETELSPEMISSGSWRDRPFKPYNFLAHGVLPDSGHLHPLLKVRSQFRQIFLEMGFTEMPTDNFIESSFWNFDALFQPQQHPARDQHDTFFLRDPAEALQLPMDYVQRVKRTHSQGGYGSQGYKYNWKLDEARKNLLRTHTTSASARALYRLAQKKPFTPVKYFSIDRVFRNETLDATHLAEFHQIEGVVADHGLTLGHLMGVLREFFTKLGITQLRFKPAYNPYTEPSMEVFSYHQGQGRPWYWGGREVSGYYGLAWVPPLPCLYPTSQAHHLDPEPVPAVPRNSTRTARLFCPASAGLKKWVEVGNSGVFRPEMLLPMGLPENVSVIAWGLSLERPTMIKYGINNIRELVGHKVNLQMVYDSPLCRLDAEPRPPPTQEAA

Target ORF information:

RefSeq Version	XM_024451419.1
Organism	Homo sapiens(human)
Definition	Homo sapiens phenylalanyl-tRNA synthetase alpha subunit (FARSA), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024451419.1

ORF Insert Sequence:

ATGGCGGATG GTCAGGTGGC GGAACTGCTG CTCCGGCGGC TGGAGGCGTC TGATGGCGGC 
CTGGACAGCG CCGAGTTGGC GGCTGAGCTG GGCATGGAGC ACCAGGCGGT GGTGGGCGCC 
GTGAAGAGCC TTCAGGCGCT GGGCGAGGTC ATCGAGGCTG AACTTCGGTC CACCAAGCAC 
TGGGAGCTTA CTGCGGAGGG CGAGGAGATT GCCCGGGAGG GCAGCCATGA GGCCCGTGTG 
TTTCGAAGCA TTCCCCCAGA GGGCCTGGCC CAGAGCGAGC TTATGCGACT GCCCAGTGGC 
AAAGTGGGCT TCAGCAAGGC CATGTCCAAC AAGTGGATTC GGGTGGACAA GAGTGCGGCT 
GACGGGCCCC GGGTGTTCCG AGTGGTGGAC AGCATGGAGG ATGAGGTGCA GCGGCGGCTC 
CAGCTGGTCC GGGGGGGACA GGCTGAGAAG CTGGGGGAGA AGGAGAGGAG CGAGCTGAGG 
AAGAGGAAGC TGTTGGCTGA AGTGACTCTG AAGACCTACT GGGTGAGCAA AGGCAGTGCC 
TTTAGTACCA GCATCTCCAA GCAAGAGACA GAGCTGAGCC CAGAGATGAT CTCCAGTGGC 
TCTTGGCGGG ACCGGCCCTT CAAGCCCTAC AACTTCTTGG CCCACGGTGT CCTCCCCGAC 
AGCGGCCACC TTCACCCGCT GCTCAAGGTC CGCTCCCAGT TCCGACAGAT CTTCCTGGAG 
ATGGGGTTCA CCGAGATGCC GACTGATAAC TTCATTGAGA GCTCCTTCTG GAACTTTGAC 
GCCCTCTTCC AGCCCCAGCA GCACCCAGCC CGTGACCAGC ACGACACCTT CTTCCTTCGA 
GATCCAGCGG AGGCCCTGCA GCTCCCAATG GACTATGTCC AGCGGGTCAA GCGGACCCAC 
TCTCAGGGCG GCTACGGCTC ACAGGGGTAC AAGTATAACT GGAAGCTGGA CGAGGCCCGG 
AAAAACCTAC TGCGAACCCA CACCACATCA GCCAGCGCCC GTGCGCTCTA CCGCCTTGCC 
CAGAAGAAGC CCTTCACTCC GGTCAAGTAC TTCTCCATCG ACCGCGTATT CCGGAATGAG 
ACCCTGGACG CCACGCACCT GGCTGAGTTC CACCAGATCG AGGGCGTGGT GGCGGATCAT 
GGTCTCACCT TGGGCCACCT CATGGGCGTT CTGCGGGAGT TCTTCACCAA GCTGGGTATC 
ACGCAACTCC GCTTCAAGCC AGCCTACAAC CCATACACAG AGCCCAGCAT GGAGGTGTTC 
AGCTACCACC AAGGTCAGGG TAGGCCCTGG TACTGGGGTG GGAGGGAGGT CTCAGGCTAC 
TATGGTTTGG CCTGGGTCCC CCCATTGCCA TGCCTGTACC CCACCTCCCA AGCTCACCAC 
CTAGACCCAG AACCTGTACC AGCAGTGCCC AGAAACTCAA CACGCACTGC CCGTCTTTTC 
TGTCCTGCCT CTGCAGGCCT GAAGAAGTGG GTGGAGGTCG GAAACTCGGG GGTCTTCCGT 
CCAGAGATGC TGCTGCCCAT GGGGCTTCCC GAGAACGTGT CGGTCATTGC CTGGGGCCTC 
TCCCTGGAGC GCCCAACGAT GATCAAATAT GGCATCAACA ATATCCGGGA GCTGGTGGGC 
CACAAGGTGA ACCTGCAGAT GGTGTATGAC AGTCCCCTGT GCCGCCTGGA TGCCGAGCCG 
AGGCCCCCTC CCACACAGGA GGCTGCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Architecture of the human interactome defines protein communities and disease networks.
Nature545(7655)505-509(2017 05)
Huttlin EL,Bruckner RJ,Paulo JA,Cannon JR,Ting L,Baltier K,Colby G,Gebreab F,Gygi MP,Parzen H,Szpyt J,Tam S,Zarraga G,Pontano-Vaites L,Swarup S,White AE,Schweppe DK,Rad R,Erickson BK,Obar RA,Guruharsha KG,Li K,Artavanis-Tsakonas S,Gygi SP,Harper JW

Host factors that interact with the pestivirus N-terminal protease, Npro, are components of the ribonucleoprotein complex.
Journal of virology88(18)10340-53(2014 Sep)
Jefferson M,Donaszi-Ivanov A,Pollen S,Dalmay T,Saalbach G,Powell PP

Clozapine-induced agranulocytosis is associated with rare HLA-DQB1 and HLA-B alleles.
Nature communications54757(2014 Sep)
Goldstein JI,Jarskog LF,Hilliard C,Alfirevic A,Duncan L,Fourches D,Huang H,Lek M,Neale BM,Ripke S,Shianna K,Szatkiewicz JP,Tropsha A,van den Oord EJ,Cascorbi I,Dettling M,Gazit E,Goff DC,Holden AL,Kelly DL,Malhotra AK,Nielsen J,Pirmohamed M,Rujescu D,Werge T,Levy DL,Josiassen RC,Kennedy JL,Lieberman JA,Daly MJ,Sullivan PF

Genome wide association study (GWAS) of Chagas cardiomyopathy in Trypanosoma cruzi seropositive subjects.
PloS one8(11)e79629(2013)
Deng X,Sabino EC,Cunha-Neto E,Ribeiro AL,Ianni B,Mady C,Busch MP,Seielstad M,

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FARSA cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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