FANCF cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	FANCF
Entrez Gene ID	2188
Full Name	Fanconi anemia complementation group F
Synonyms	FAF
General protein information	Preferred Names Fanconi anemia complementation group F Names Fanconi anemia group F protein
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 11 Map Location: 11p14.3
Summary	The Fanconi anemia complementation group (FANC) currently includes FANCA, FANCB, FANCC, FANCD1 (also called BRCA2), FANCD2, FANCE, FANCF, FANCG, FANCI, FANCJ (also called BRIP1), FANCL, FANCM and FANCN (also called PALB2). The previously defined group FANCH is the same as FANCA. Fanconi anemia is a genetically heterogeneous recessive disorder characterized by cytogenetic instability, hypersensitivity to DNA crosslinking agents, increased chromosomal breakage, and defective DNA repair. The members of the Fanconi anemia complementation group do not share sequence similarity; they are related by their assembly into a common nuclear protein complex. This gene encodes the protein for complementation group F. [provided by RefSeq, Jul 2008].
Disorder MIM:	613897
Disorder Html:	Fanconi anemia, complementation group F, 603467 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_022725.3	NP_073562.1	Fanconi anemia group F protein
NM_022725.4	NP_073562.1	Fanconi anemia group F protein

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	fancf	NP_001038699.2
	LOC704048	XP_001092387.2
Xenopus tropicalis (tropical clawed frog)	fancf	NP_001120095.1
Homo sapiens (human)	FANCF	NP_073562.1
Pan troglodytes (chimpanzee)	FANCF	XP_001169698.2
Bos taurus (cattle)	FANCF	NP_001098895.1
Mus musculus (house mouse)	Fancf	NP_001108559.1
Gallus gallus (chicken)	FANCF	XP_004941387.1
Rattus norvegicus (Norway rat)	Fancf	XP_574453.2

Gene Ontology
Bibliography

Related articles in PubMed

Loss of heterozygosity in FANCG, FANCF and BRIP1 from head and neck squamous cell carcinoma of the oral cavity.
T?rke C, Horn S, Petto C, Labudde D, Lauer G, Wittenburg G
International journal of oncology50(6)2207-2220(2017 Jun)

Phenotypic variability in patients with Fanconi anemia and biallelic FANCF mutations.
Tryon R, Zierhut H, MacMillan ML, Wagner JE
American journal of medical genetics. Part A173(1)260-263(2017 Jan)

Promoter Hypermethylation of FANCF and Susceptibility and Prognosis of Epithelial Ovarian Cancer.
Ding JJ, Wang G, Shi WX, Zhou HH, Zhao EF
Reproductive sciences (Thousand Oaks, Calif.)23(1)24-30(2016 Jan)

RNA interferences targeting the Fanconi anemia/BRCA pathway upstream genes reverse cisplatin resistance in drug-resistant lung cancer cells.
Dai CH, Li J, Chen P, Jiang HG, Wu M, Chen YC
Journal of biomedical science2277(2015 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

we report three patients who illustrate the clinical variability within the FA-F group. This analysis suggests a more severe phenotype for those with the common c.484_485delCT mutation.
Title: Phenotypic variability in patients with Fanconi anemia and biallelic FANCF mutations.

CpG island methylation of FANCF gene promoter region is strongly associated with the susceptibility and clinicopathologic features of epithelial ovarian cancer.
Title: Promoter Hypermethylation of FANCF and Susceptibility and Prognosis of Epithelial Ovarian Cancer.

careful examination of three electively aborted fetuses in one family and one affected girl in the other indicated an association of the FANCF loss-of-function mutation with a severe phenotype characterized by multiple malformations
Title: Clinical aspects of Fanconi anemia individuals with the same mutation of FANCF identified by next generation sequencing.

Using small interfering RNA (siRNA), knockdown of FANCF, FANCL, or FANCD2 inhibited function of the FA/BRCA pathway in A549, A549/DDP and SK-MES-1 cells, and potentiated sensitivity of the three cells to cisplatin.
Title: RNA interferences targeting the Fanconi anemia/BRCA pathway upstream genes reverse cisplatin resistance in drug-resistant lung cancer cells.

Data suggest that the Fanconi anemia group F protein/BRCA1/2 proteins pathway may be a new target to reverse adriamycin (ADR) resistance in leukemia treatment.
Title: The Fanconi anemia/BRCA pathway is involved in DNA interstrand cross-link repair of adriamycin-resistant leukemia cells.

The following FANCF gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the FANCF cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu26174	NM_022725.4 Latest version!	Homo sapiens FA complementation group F (FANCF), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
View Old Accession Versions >>
OHu26174	NM_022725.3	Homo sapiens FA complementation group F (FANCF), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

FANCF ( NM_022725.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_073562.1 Homo sapiens FA complementation group F (FANCF), mRNA.

Price & Availability↑

CloneID	OHu26174
Clone ID Related Accession (Same CDS sequence)	NM_022725.3 , NM_022725.4
Accession Version	NM_022725.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1125bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-07
Organism	Homo sapiens(human)
Product	Fanconi anemia group F protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC103801.2. This sequence is a reference standard in the RefSeqGene project. On Feb 15, 2008 this sequence version replaced NM_022725.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##RefSeq-Attributes-START## RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGAATCCC TTCTGCAGCA CCTGGATCGC TTTTCCGAGC TTCTGGCGGT CTCAAGCACT 
ACCTACGTCA GCACCTGGGA CCCCGCCACC GTGCGCCGGG CCTTGCAGTG GGCGCGCTAC 
CTGCGCCACA TCCATCGGCG CTTTGGTCGG CATGGCCCCA TTCGCACGGC TCTGGAGCGG 
CGGCTGCACA ACCAGTGGAG GCAAGAGGGC GGCTTTGGGC GGGGTCCAGT TCCGGGATTA 
GCGAACTTCC AGGCCCTCGG TCACTGTGAC GTCCTGCTCT CTCTGCGCCT GCTGGAGAAC 
CGGGCCCTCG GGGATGCAGC TCGTTACCAC CTGGTGCAGC AACTCTTTCC CGGCCCGGGC 
GTCCGGGACG CCGATGAGGA GACACTCCAA GAGAGCCTGG CCCGCCTTGC CCGCCGGCGG 
TCTGCGGTGC ACATGCTGCG CTTCAATGGC TATAGAGAGA ACCCAAATCT CCAGGAGGAC 
TCTCTGATGA AGACCCAGGC GGAGCTGCTG CTGGAGCGTC TGCAGGAGGT GGGGAAGGCC 
GAAGCGGAGC GTCCCGCCAG GTTTCTCAGC AGCCTGTGGG AGCGCTTGCC TCAGAACAAC 
TTCCTGAAGG TGATAGCGGT GGCGCTGTTG CAGCCGCCTT TGTCTCGTCG GCCCCAAGAA 
GAGTTGGAAC CCGGCATCCA CAAATCACCT GGAGAGGGGA GCCAAGTGCT AGTCCACTGG 
CTTCTGGGGA ATTCGGAAGT CTTTGCTGCC TTTTGTCGCG CCCTCCCAGC CGGGCTTTTG 
ACTTTAGTGA CTAGCCGCCA CCCAGCGCTG TCTCCTGTCT ATCTGGGTCT GCTAACAGAC 
TGGGGTCAAC GTTTGCACTA TGACCTTCAG AAAGGCATTT GGGTTGGAAC TGAGTCCCAA 
GATGTGCCCT GGGAGGAGTT GCACAATAGG TTTCAAAGCC TCTGTCAGGC CCCTCCACCT 
CTGAAAGATA AAGTTCTAAC TGCCCTGGAG ACCTGTAAAG CGCAGGATGG AGATTTTGAA 
GTACCTGGTC TTAGCATCTG GACAGACCTC TTATTAGCTC TTCGTAGTGG TGCATTTAGG 
AAAAGACAAG TTTTGGGTCT CAGCGCAGGC CTCAGTTCTG TATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_073562.1
CDS	32..1156
Translation	MESLLQHLDRFSELLAVSSTTYVSTWDPATVRRALQWARYLRHIHRRFGRHGPIRTALERRLHNQWRQEGGFGRGPVPGLANFQALGHCDVLLSLRLLENRALGDAARYHLVQQLFPGPGVRDADEETLQESLARLARRRSAVHMLRFNGYRENPNLQEDSLMKTQAELLLERLQEVGKAEAERPARFLSSLWERLPQNNFLKVIAVALLQPPLSRRPQEELEPGIHKSPGEGSQVLVHWLLGNSEVFAAFCRALPAGLLTLVTSRHPALSPVYLGLLTDWGQRLHYDLQKGIWVGTESQDVPWEELHNRFQSLCQAPPPLKDKVLTALETCKAQDGDFEVPGLSIWTDLLLALRSGAFRKRQVLGLSAGLSSV

Target ORF information:

RefSeq Version	NM_022725.3
Organism	Homo sapiens(human)
Definition	Homo sapiens FA complementation group F (FANCF), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_022725.3

ORF Insert Sequence:

ATGGAATCCC TTCTGCAGCA CCTGGATCGC TTTTCCGAGC TTCTGGCGGT CTCAAGCACT 
ACCTACGTCA GCACCTGGGA CCCCGCCACC GTGCGCCGGG CCTTGCAGTG GGCGCGCTAC 
CTGCGCCACA TCCATCGGCG CTTTGGTCGG CATGGCCCCA TTCGCACGGC TCTGGAGCGG 
CGGCTGCACA ACCAGTGGAG GCAAGAGGGC GGCTTTGGGC GGGGTCCAGT TCCGGGATTA 
GCGAACTTCC AGGCCCTCGG TCACTGTGAC GTCCTGCTCT CTCTGCGCCT GCTGGAGAAC 
CGGGCCCTCG GGGATGCAGC TCGTTACCAC CTGGTGCAGC AACTCTTTCC CGGCCCGGGC 
GTCCGGGACG CCGATGAGGA GACACTCCAA GAGAGCCTGG CCCGCCTTGC CCGCCGGCGG 
TCTGCGGTGC ACATGCTGCG CTTCAATGGC TATAGAGAGA ACCCAAATCT CCAGGAGGAC 
TCTCTGATGA AGACCCAGGC GGAGCTGCTG CTGGAGCGTC TGCAGGAGGT GGGGAAGGCC 
GAAGCGGAGC GTCCCGCCAG GTTTCTCAGC AGCCTGTGGG AGCGCTTGCC TCAGAACAAC 
TTCCTGAAGG TGATAGCGGT GGCGCTGTTG CAGCCGCCTT TGTCTCGTCG GCCCCAAGAA 
GAGTTGGAAC CCGGCATCCA CAAATCACCT GGAGAGGGGA GCCAAGTGCT AGTCCACTGG 
CTTCTGGGGA ATTCGGAAGT CTTTGCTGCC TTTTGTCGCG CCCTCCCAGC CGGGCTTTTG 
ACTTTAGTGA CTAGCCGCCA CCCAGCGCTG TCTCCTGTCT ATCTGGGTCT GCTAACAGAC 
TGGGGTCAAC GTTTGCACTA TGACCTTCAG AAAGGCATTT GGGTTGGAAC TGAGTCCCAA 
GATGTGCCCT GGGAGGAGTT GCACAATAGG TTTCAAAGCC TCTGTCAGGC CCCTCCACCT 
CTGAAAGATA AAGTTCTAAC TGCCCTGGAG ACCTGTAAAG CGCAGGATGG AGATTTTGAA 
GTACCTGGTC TTAGCATCTG GACAGACCTC TTATTAGCTC TTCGTAGTGG TGCATTTAGG 
AAAAGACAAG TTTTGGGTCT CAGCGCAGGC CTCAGTTCTG TATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FANCF ( NM_022725.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_073562.1 Homo sapiens FA complementation group F (FANCF), mRNA.

Price & Availability↑

CloneID	OHu26174
Clone ID Related Accession (Same CDS sequence)	NM_022725.3 , NM_022725.4
Accession Version	NM_022725.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1125bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-09
Organism	Homo sapiens(human)
Product	Fanconi anemia group F protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC103801.2. On Aug 31, 2019 this sequence version replaced NM_022725.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript is intronless :: AK001716.2, AK023153.1 [ECO:0000345] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGAATCCC TTCTGCAGCA CCTGGATCGC TTTTCCGAGC TTCTGGCGGT CTCAAGCACT 
ACCTACGTCA GCACCTGGGA CCCCGCCACC GTGCGCCGGG CCTTGCAGTG GGCGCGCTAC 
CTGCGCCACA TCCATCGGCG CTTTGGTCGG CATGGCCCCA TTCGCACGGC TCTGGAGCGG 
CGGCTGCACA ACCAGTGGAG GCAAGAGGGC GGCTTTGGGC GGGGTCCAGT TCCGGGATTA 
GCGAACTTCC AGGCCCTCGG TCACTGTGAC GTCCTGCTCT CTCTGCGCCT GCTGGAGAAC 
CGGGCCCTCG GGGATGCAGC TCGTTACCAC CTGGTGCAGC AACTCTTTCC CGGCCCGGGC 
GTCCGGGACG CCGATGAGGA GACACTCCAA GAGAGCCTGG CCCGCCTTGC CCGCCGGCGG 
TCTGCGGTGC ACATGCTGCG CTTCAATGGC TATAGAGAGA ACCCAAATCT CCAGGAGGAC 
TCTCTGATGA AGACCCAGGC GGAGCTGCTG CTGGAGCGTC TGCAGGAGGT GGGGAAGGCC 
GAAGCGGAGC GTCCCGCCAG GTTTCTCAGC AGCCTGTGGG AGCGCTTGCC TCAGAACAAC 
TTCCTGAAGG TGATAGCGGT GGCGCTGTTG CAGCCGCCTT TGTCTCGTCG GCCCCAAGAA 
GAGTTGGAAC CCGGCATCCA CAAATCACCT GGAGAGGGGA GCCAAGTGCT AGTCCACTGG 
CTTCTGGGGA ATTCGGAAGT CTTTGCTGCC TTTTGTCGCG CCCTCCCAGC CGGGCTTTTG 
ACTTTAGTGA CTAGCCGCCA CCCAGCGCTG TCTCCTGTCT ATCTGGGTCT GCTAACAGAC 
TGGGGTCAAC GTTTGCACTA TGACCTTCAG AAAGGCATTT GGGTTGGAAC TGAGTCCCAA 
GATGTGCCCT GGGAGGAGTT GCACAATAGG TTTCAAAGCC TCTGTCAGGC CCCTCCACCT 
CTGAAAGATA AAGTTCTAAC TGCCCTGGAG ACCTGTAAAG CGCAGGATGG AGATTTTGAA 
GTACCTGGTC TTAGCATCTG GACAGACCTC TTATTAGCTC TTCGTAGTGG TGCATTTAGG 
AAAAGACAAG TTTTGGGTCT CAGCGCAGGC CTCAGTTCTG TATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_073562.1
CDS	14..1138
Translation	MESLLQHLDRFSELLAVSSTTYVSTWDPATVRRALQWARYLRHIHRRFGRHGPIRTALERRLHNQWRQEGGFGRGPVPGLANFQALGHCDVLLSLRLLENRALGDAARYHLVQQLFPGPGVRDADEETLQESLARLARRRSAVHMLRFNGYRENPNLQEDSLMKTQAELLLERLQEVGKAEAERPARFLSSLWERLPQNNFLKVIAVALLQPPLSRRPQEELEPGIHKSPGEGSQVLVHWLLGNSEVFAAFCRALPAGLLTLVTSRHPALSPVYLGLLTDWGQRLHYDLQKGIWVGTESQDVPWEELHNRFQSLCQAPPPLKDKVLTALETCKAQDGDFEVPGLSIWTDLLLALRSGAFRKRQVLGLSAGLSSV

Target ORF information:

RefSeq Version	NM_022725.4
Organism	Homo sapiens(human)
Definition	Homo sapiens FA complementation group F (FANCF), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_022725.4

ORF Insert Sequence:

ATGGAATCCC TTCTGCAGCA CCTGGATCGC TTTTCCGAGC TTCTGGCGGT CTCAAGCACT 
ACCTACGTCA GCACCTGGGA CCCCGCCACC GTGCGCCGGG CCTTGCAGTG GGCGCGCTAC 
CTGCGCCACA TCCATCGGCG CTTTGGTCGG CATGGCCCCA TTCGCACGGC TCTGGAGCGG 
CGGCTGCACA ACCAGTGGAG GCAAGAGGGC GGCTTTGGGC GGGGTCCAGT TCCGGGATTA 
GCGAACTTCC AGGCCCTCGG TCACTGTGAC GTCCTGCTCT CTCTGCGCCT GCTGGAGAAC 
CGGGCCCTCG GGGATGCAGC TCGTTACCAC CTGGTGCAGC AACTCTTTCC CGGCCCGGGC 
GTCCGGGACG CCGATGAGGA GACACTCCAA GAGAGCCTGG CCCGCCTTGC CCGCCGGCGG 
TCTGCGGTGC ACATGCTGCG CTTCAATGGC TATAGAGAGA ACCCAAATCT CCAGGAGGAC 
TCTCTGATGA AGACCCAGGC GGAGCTGCTG CTGGAGCGTC TGCAGGAGGT GGGGAAGGCC 
GAAGCGGAGC GTCCCGCCAG GTTTCTCAGC AGCCTGTGGG AGCGCTTGCC TCAGAACAAC 
TTCCTGAAGG TGATAGCGGT GGCGCTGTTG CAGCCGCCTT TGTCTCGTCG GCCCCAAGAA 
GAGTTGGAAC CCGGCATCCA CAAATCACCT GGAGAGGGGA GCCAAGTGCT AGTCCACTGG 
CTTCTGGGGA ATTCGGAAGT CTTTGCTGCC TTTTGTCGCG CCCTCCCAGC CGGGCTTTTG 
ACTTTAGTGA CTAGCCGCCA CCCAGCGCTG TCTCCTGTCT ATCTGGGTCT GCTAACAGAC 
TGGGGTCAAC GTTTGCACTA TGACCTTCAG AAAGGCATTT GGGTTGGAAC TGAGTCCCAA 
GATGTGCCCT GGGAGGAGTT GCACAATAGG TTTCAAAGCC TCTGTCAGGC CCCTCCACCT 
CTGAAAGATA AAGTTCTAAC TGCCCTGGAG ACCTGTAAAG CGCAGGATGG AGATTTTGAA 
GTACCTGGTC TTAGCATCTG GACAGACCTC TTATTAGCTC TTCGTAGTGG TGCATTTAGG 
AAAAGACAAG TTTTGGGTCT CAGCGCAGGC CTCAGTTCTG TATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Loss of heterozygosity in FANCG, FANCF and BRIP1 from head and neck squamous cell carcinoma of the oral cavity.
International journal of oncology50(6)2207-2220(2017 Jun)
T?rke C,Horn S,Petto C,Labudde D,Lauer G,Wittenburg G

Phenotypic variability in patients with Fanconi anemia and biallelic FANCF mutations.
American journal of medical genetics. Part A173(1)260-263(2017 Jan)
Tryon R,Zierhut H,MacMillan ML,Wagner JE

Promoter Hypermethylation of FANCF and Susceptibility and Prognosis of Epithelial Ovarian Cancer.
Reproductive sciences (Thousand Oaks, Calif.)23(1)24-30(2016 Jan)
Ding JJ,Wang G,Shi WX,Zhou HH,Zhao EF

RNA interferences targeting the Fanconi anemia/BRCA pathway upstream genes reverse cisplatin resistance in drug-resistant lung cancer cells.
Journal of biomedical science2277(2015 Sep)
Dai CH,Li J,Chen P,Jiang HG,Wu M,Chen YC

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FANCF cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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