ETFDH cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	ETFDH
Entrez Gene ID	2110
Full Name	electron transfer flavoprotein dehydrogenase
Synonyms	ETFQO,MADD
General protein information	Preferred Names electron transfer flavoprotein dehydrogenase Names electron transfer flavoprotein-ubiquinone oxidoreductase, mitochondrial ETF dehydrogenase ETF-QO ETF-ubiquinone oxidoreductase electron-transferring-flavoprotein dehydrogenase
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 4 Map Location: 4q32.1
Summary	This gene encodes a component of the electron-transfer system in mitochondria and is essential for electron transfer from a number of mitochondrial flavin-containing dehydrogenases to the main respiratory chain. Mutations in this gene are associated with glutaric acidemia. Alternatively spliced transcript variants that encode distinct isoforms have been observed. [provided by RefSeq, Aug 2013].
Disorder MIM:	231675
Disorder Html:	Glutaric acidemia IIC, 231680 (3)

mRNA and Protein(s)

mRNA	Protein	Name
XM_024453935.1	XP_024309703.1	electron transfer flavoprotein-ubiquinone oxidoreductase, mitochondrial isoform X1
NM_001281738.1	NP_001268667.1	electron transfer flavoprotein-ubiquinone oxidoreductase, mitochondrial isoform 3
NM_004453.3	NP_004444.2	electron transfer flavoprotein-ubiquinone oxidoreductase, mitochondrial isoform 1 precursor
NM_004453.4	NP_004444.2	electron transfer flavoprotein-ubiquinone oxidoreductase, mitochondrial isoform 1 precursor
NM_001281737.1	NP_001268666.1	electron transfer flavoprotein-ubiquinone oxidoreductase, mitochondrial isoform 2

Pathways from BioSystems

Homologies

	ETFDH	XP_001097120.1
Mus musculus (house mouse)	Etfdh	NP_080070.2
Danio rerio (zebrafish)	etfdh	NP_001004598.1
Caenorhabditis elegans (roundworm)	let-721	NP_498415.2
Saccharomyces cerevisiae (baker's yeast)	CIR2	NP_015001.1
Magnaporthe oryzae (rice blast fungus)	MGG_08880	XP_003713904.1
Gallus gallus (chicken)	ETFDH	NP_001026705.1
Eremothecium gossypii	AGOS_ADR387W	NP_984483.2
Schizosaccharomyces pombe (fission yeast)	SPAC20G8.04c	NP_593321.1
Arabidopsis thaliana (thale cress)	ETFQO	NP_181868.1
Xenopus tropicalis (tropical clawed frog)	etfdh	NP_001005631.1
Rattus norvegicus (Norway rat)	Etfdh	NP_942037.2
Drosophila melanogaster (fruit fly)	Etf-QO	NP_610536.1
Kluyveromyces lactis	KLLA0C12991g	XP_452779.1
Neurospora crassa	NCU04768	XP_960217.1
Homo sapiens (human)	ETFDH	NP_004444.2
Canis lupus familiaris (dog)	ETFDH	XP_853781.2
Bos taurus (cattle)	ETFDH	NP_001070598.1
Oryza sativa (rice)	Os10g0516300	NP_001065062.1
Pan troglodytes (chimpanzee)	ETFDH	XP_517508.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005894	XP_315923.3

Gene Ontology
Bibliography

Related articles in PubMed

Neurite growth could be impaired by ETFDH mutation but restored by mitochondrial cofactors.
Liang WC, Lin YF, Liu TY, Chang SC, Chen BH, Nishino I, Jong YJ
Muscle & nerve56(3)479-485(2017 Sep)

[Clinical features and gene mutations in a patient with multiple aeyl-CoA dehydrogenase deficiency with severe fatty liver].
Dai D, Wen F, Zhou S, Chen S
Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese journal of medical genetics33(2)191-4(2016 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Neurite shortening and impairment in neurite growth was caused by a mutation in ETFDH.
Title: Neurite growth could be impaired by ETFDH mutation but restored by mitochondrial cofactors.

This study identified three novel compound heterozygous mutations of ETFDH gene in patients with late-onset multiple acyl-CoA dehydrogenase deficiency, and discussed the significant clinical heterogeneity among patients with similar genotype.
Title: Significant clinical heterogeneity with similar ETFDH genotype in three Chinese patients with late-onset multiple acyl-CoA dehydrogenase deficiency.

Mtation c.250G>A and mutation c.353G>T in the ETFDH gene are associate with multiple aeyl-CoA dehydrogenase deficiency with severe fatty liver.
Title: [Clinical features and gene mutations in a patient with multiple aeyl-CoA dehydrogenase deficiency with severe fatty liver].

ETFDH mutation is the causative gene in patients with adult-onset multiple acyl-CoA dehydrogenase deficiency with severe sensory neuropathy.
Title: Severe sensory neuropathy in patients with adult-onset multiple acyl-CoA dehydrogenase deficiency.

Mutations in SLC22A5 and ETFDH are associated with riboflavin responsive-multiple acyl-CoA dehydrogenase deficiency.
Title: [Mutation analysis for a family affected with riboflavin responsive-multiple acyl-CoA dehydrogenase deficiency].

The following ETFDH gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ETFDH cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu20997	NM_004453.4 Latest version!	Homo sapiens electron transfer flavoprotein dehydrogenase (ETFDH), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu15675	NM_001281738.1 Latest version!	Homo sapiens electron transfer flavoprotein dehydrogenase (ETFDH), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00
OHu15675	XM_024453935.1 Latest version!	Homo sapiens electron transfer flavoprotein dehydrogenase (ETFDH), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00
OHu15884	NM_001281737.1 Latest version!	Homo sapiens electron transfer flavoprotein dehydrogenase (ETFDH), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
View Old Accession Versions >>
OHu20997	NM_004453.3	Homo sapiens electron transfer flavoprotein dehydrogenase (ETFDH), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ETFDH ( NM_004453.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_004444.2 Homo sapiens electron transfer flavoprotein dehydrogenase (ETFDH), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu20997
Clone ID Related Accession (Same CDS sequence)	NM_004453.3 , NM_004453.4
Accession Version	NM_004453.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1854bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-23
Organism	Homo sapiens(human)
Product	electron transfer flavoprotein-ubiquinone oxidoreductase, mitochondrial isoform 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA798232.1, BX957055.1, BC011890.1 and AK293737.1. This sequence is a reference standard in the RefSeqGene project. On Aug 2, 2013 this sequence version replaced NM_004453.2. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest protein (isoform 1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.11125.1, SRR1660809.165462.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCTGGTGC CGCTAGCCAA GCTGTCCTGC CTGGCATATC AGTGCTTTCA TGCCTTAAAA 
ATTAAGAAAA ATTATCTACC TCTATGTGCT ACAAGATGGT CTTCAACTTC TACTGTGCCT 
CGAATTACTA CCCATTATAC TATTTATCCC CGGGATAAGG ACAAGAGATG GGAAGGAGTG 
AACATGGAAA GGTTTGCAGA AGAAGCAGAT GTTGTAATAG TTGGTGCAGG CCCTGCAGGG 
CTCTCTGCAG CTGTTCGTCT AAAACAGTTG GCTGTGGCAC ATGAAAAGGA CATCCGTGTG 
TGTCTAGTGG AGAAAGCTGC CCAGATAGGA GCTCATACTC TCTCAGGGGC TTGCCTTGAT 
CCAGGTGCTT TTAAAGAACT CTTCCCAGAC TGGAAAGAGA AGGGGGCTCC ACTTAACACT 
CCTGTAACAG AAGACAGATT TGGAATTTTA ACAGAGAAAT ACAGAATTCC TGTGCCAATT 
CTTCCAGGGC TTCCAATGAA TAATCATGGC AATTACATTG TACGCTTGGG ACATTTAGTG 
AGCTGGATGG GCGAACAAGC AGAAGCCCTT GGTGTTGAAG TATACCCTGG TTATGCAGCT 
GCTGAGGTCC TTTTTCATGA TGATGGTAGT GTAAAAGGAA TTGCCACTAA CGATGTAGGG 
ATACAAAAGG ATGGTGCACC AAAGGCAACA TTTGAGAGAG GACTGGAACT ACATGCTAAA 
GTCACAATTT TTGCAGAAGG TTGCCATGGA CATCTAGCCA AGCAACTATA TAAGAAGTTT 
GATTTGAGAG CAAATTGTGA ACCTCAAACC TACGGGATTG GACTGAAGGA GTTATGGGTT 
ATTGATGAAA AGAACTGGAA ACCTGGGAGA GTAGATCACA CTGTTGGTTG GCCCTTGGAC 
AGACATACCT ATGGAGGATC TTTCCTCTAT CATTTGAATG AAGGTGAACC CCTAGTAGCT 
CTTGGTCTTG TGGTTGGTCT AGACTATCAG AATCCATACC TGAGTCCATT TAGAGAGTTC 
CAAAGGTGGA AACACCATCC TAGCATTCGG CCAACCTTGG AAGGTGGAAA AAGGATTGCA 
TACGGAGCCA GAGCTCTCAA TGAAGGTGGC TTTCAGTCTA TACCAAAACT CACCTTTCCT 
GGTGGTTTAC TAATTGGTTG TAGTCCTGGT TTTATGAATG TTCCCAAGAT CAAAGGTACT 
CACACAGCAA TGAAAAGTGG AATTTTAGCA GCAGAATCTA TTTTTAATCA ACTAACTAGT 
GAAAATCTCC AATCAAAGAC AATAGGACTC CATGTAACTG AATATGAGGA CAATTTGAAG 
AACTCATGGG TATGGAAAGA GCTATATTCT GTTAGAAATA TAAGACCGTC CTGCCACGGA 
GTACTGGGTG TATATGGAGG GATGATTTAC ACTGGAATCT TTTACTGGAT ATTGAGAGGA 
ATGGAGCCGT GGACTCTGAA ACATAAAGGT TCTGACTTTG AACGGCTCAA GCCAGCCAAG 
GATTGCACAC CTATTGAGTA TCCAAAACCC GATGGACAGA TCAGTTTTGA CCTCTTGTCA 
TCTGTGGCTC TGAGTGGTAC TAATCATGAA CATGACCAGC CGGCACACTT AACCTTAAGG 
GATGACAGTA TACCTGTAAA TAGAAATCTG TCGATATATG ATGGGCCCGA GCAGCGATTC 
TGTCCTGCAG GAGTTTATGA ATTTGTACCT GTGGAACAAG GTGATGGATT TCGGTTACAG 
ATAAATGCTC AGAACTGTGT ACATTGTAAA ACATGTGATA TTAAAGATCC AAGTCAGAAT 
ATTAACTGGG TGGTACCTGA AGGTGGAGGA GGACCTGCTT ACAATGGAAT GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004444.2
CDS	357..2210
Translation	MLVPLAKLSCLAYQCFHALKIKKNYLPLCATRWSSTSTVPRITTHYTIYPRDKDKRWEGVNMERFAEEADVVIVGAGPAGLSAAVRLKQLAVAHEKDIRVCLVEKAAQIGAHTLSGACLDPGAFKELFPDWKEKGAPLNTPVTEDRFGILTEKYRIPVPILPGLPMNNHGNYIVRLGHLVSWMGEQAEALGVEVYPGYAAAEVLFHDDGSVKGIATNDVGIQKDGAPKATFERGLELHAKVTIFAEGCHGHLAKQLYKKFDLRANCEPQTYGIGLKELWVIDEKNWKPGRVDHTVGWPLDRHTYGGSFLYHLNEGEPLVALGLVVGLDYQNPYLSPFREFQRWKHHPSIRPTLEGGKRIAYGARALNEGGFQSIPKLTFPGGLLIGCSPGFMNVPKIKGTHTAMKSGILAAESIFNQLTSENLQSKTIGLHVTEYEDNLKNSWVWKELYSVRNIRPSCHGVLGVYGGMIYTGIFYWILRGMEPWTLKHKGSDFERLKPAKDCTPIEYPKPDGQISFDLLSSVALSGTNHEHDQPAHLTLRDDSIPVNRNLSIYDGPEQRFCPAGVYEFVPVEQGDGFRLQINAQNCVHCKTCDIKDPSQNINWVVPEGGGGPAYNGM

Target ORF information:

RefSeq Version	NM_004453.3
Organism	Homo sapiens(human)
Definition	Homo sapiens electron transfer flavoprotein dehydrogenase (ETFDH), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004453.3

ORF Insert Sequence:

ATGCTGGTGC CGCTAGCCAA GCTGTCCTGC CTGGCATATC AGTGCTTTCA TGCCTTAAAA 
ATTAAGAAAA ATTATCTACC TCTATGTGCT ACAAGATGGT CTTCAACTTC TACTGTGCCT 
CGAATTACTA CCCATTATAC TATTTATCCC CGGGATAAGG ACAAGAGATG GGAAGGAGTG 
AACATGGAAA GGTTTGCAGA AGAAGCAGAT GTTGTAATAG TTGGTGCAGG CCCTGCAGGG 
CTCTCTGCAG CTGTTCGTCT AAAACAGTTG GCTGTGGCAC ATGAAAAGGA CATCCGTGTG 
TGTCTAGTGG AGAAAGCTGC CCAGATAGGA GCTCATACTC TCTCAGGGGC TTGCCTTGAT 
CCAGGTGCTT TTAAAGAACT CTTCCCAGAC TGGAAAGAGA AGGGGGCTCC ACTTAACACT 
CCTGTAACAG AAGACAGATT TGGAATTTTA ACAGAGAAAT ACAGAATTCC TGTGCCAATT 
CTTCCAGGGC TTCCAATGAA TAATCATGGC AATTACATTG TACGCTTGGG ACATTTAGTG 
AGCTGGATGG GCGAACAAGC AGAAGCCCTT GGTGTTGAAG TATACCCTGG TTATGCAGCT 
GCTGAGGTCC TTTTTCATGA TGATGGTAGT GTAAAAGGAA TTGCCACTAA CGATGTAGGG 
ATACAAAAGG ATGGTGCACC AAAGGCAACA TTTGAGAGAG GACTGGAACT ACATGCTAAA 
GTCACAATTT TTGCAGAAGG TTGCCATGGA CATCTAGCCA AGCAACTATA TAAGAAGTTT 
GATTTGAGAG CAAATTGTGA ACCTCAAACC TACGGGATTG GACTGAAGGA GTTATGGGTT 
ATTGATGAAA AGAACTGGAA ACCTGGGAGA GTAGATCACA CTGTTGGTTG GCCCTTGGAC 
AGACATACCT ATGGAGGATC TTTCCTCTAT CATTTGAATG AAGGTGAACC CCTAGTAGCT 
CTTGGTCTTG TGGTTGGTCT AGACTATCAG AATCCATACC TGAGTCCATT TAGAGAGTTC 
CAAAGGTGGA AACACCATCC TAGCATTCGG CCAACCTTGG AAGGTGGAAA AAGGATTGCA 
TACGGAGCCA GAGCTCTCAA TGAAGGTGGC TTTCAGTCTA TACCAAAACT CACCTTTCCT 
GGTGGTTTAC TAATTGGTTG TAGTCCTGGT TTTATGAATG TTCCCAAGAT CAAAGGTACT 
CACACAGCAA TGAAAAGTGG AATTTTAGCA GCAGAATCTA TTTTTAATCA ACTAACTAGT 
GAAAATCTCC AATCAAAGAC AATAGGACTC CATGTAACTG AATATGAGGA CAATTTGAAG 
AACTCATGGG TATGGAAAGA GCTATATTCT GTTAGAAATA TAAGACCGTC CTGCCACGGA 
GTACTGGGTG TATATGGAGG GATGATTTAC ACTGGAATCT TTTACTGGAT ATTGAGAGGA 
ATGGAGCCGT GGACTCTGAA ACATAAAGGT TCTGACTTTG AACGGCTCAA GCCAGCCAAG 
GATTGCACAC CTATTGAGTA TCCAAAACCC GATGGACAGA TCAGTTTTGA CCTCTTGTCA 
TCTGTGGCTC TGAGTGGTAC TAATCATGAA CATGACCAGC CGGCACACTT AACCTTAAGG 
GATGACAGTA TACCTGTAAA TAGAAATCTG TCGATATATG ATGGGCCCGA GCAGCGATTC 
TGTCCTGCAG GAGTTTATGA ATTTGTACCT GTGGAACAAG GTGATGGATT TCGGTTACAG 
ATAAATGCTC AGAACTGTGT ACATTGTAAA ACATGTGATA TTAAAGATCC AAGTCAGAAT 
ATTAACTGGG TGGTACCTGA AGGTGGAGGA GGACCTGCTT ACAATGGAAT GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ETFDH ( NM_004453.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_004444.2 Homo sapiens electron transfer flavoprotein dehydrogenase (ETFDH), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu20997
Clone ID Related Accession (Same CDS sequence)	NM_004453.3 , NM_004453.4
Accession Version	NM_004453.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1854bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-31
Organism	Homo sapiens(human)
Product	electron transfer flavoprotein-ubiquinone oxidoreductase, mitochondrial isoform 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA798232.1, BX957055.1, BC011890.1, AK293737.1 and AC107219.5. This sequence is a reference standard in the RefSeqGene project. On May 17, 2019 this sequence version replaced NM_004453.3. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest protein (isoform 1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.176600.1, SRR1660803.223499.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta MANE Ensembl match :: ENST00000511912.6/ ENSP00000426638.1 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCTGGTGC CGCTAGCCAA GCTGTCCTGC CTGGCATATC AGTGCTTTCA TGCCTTAAAA 
ATTAAGAAAA ATTATCTACC TCTATGTGCT ACAAGATGGT CTTCAACTTC TACTGTGCCT 
CGAATTACTA CCCATTATAC TATTTATCCC CGGGATAAGG ACAAGAGATG GGAAGGAGTG 
AACATGGAAA GGTTTGCAGA AGAAGCAGAT GTTGTAATAG TTGGTGCAGG CCCTGCAGGG 
CTCTCTGCAG CTGTTCGTCT AAAACAGTTG GCTGTGGCAC ATGAAAAGGA CATCCGTGTG 
TGTCTAGTGG AGAAAGCTGC CCAGATAGGA GCTCATACTC TCTCAGGGGC TTGCCTTGAT 
CCAGGTGCTT TTAAAGAACT CTTCCCAGAC TGGAAAGAGA AGGGGGCTCC ACTTAACACT 
CCTGTAACAG AAGACAGATT TGGAATTTTA ACAGAGAAAT ACAGAATTCC TGTGCCAATT 
CTTCCAGGGC TTCCAATGAA TAATCATGGC AATTACATTG TACGCTTGGG ACATTTAGTG 
AGCTGGATGG GCGAACAAGC AGAAGCCCTT GGTGTTGAAG TATACCCTGG TTATGCAGCT 
GCTGAGGTCC TTTTTCATGA TGATGGTAGT GTAAAAGGAA TTGCCACTAA CGATGTAGGG 
ATACAAAAGG ATGGTGCACC AAAGGCAACA TTTGAGAGAG GACTGGAACT ACATGCTAAA 
GTCACAATTT TTGCAGAAGG TTGCCATGGA CATCTAGCCA AGCAACTATA TAAGAAGTTT 
GATTTGAGAG CAAATTGTGA ACCTCAAACC TACGGGATTG GACTGAAGGA GTTATGGGTT 
ATTGATGAAA AGAACTGGAA ACCTGGGAGA GTAGATCACA CTGTTGGTTG GCCCTTGGAC 
AGACATACCT ATGGAGGATC TTTCCTCTAT CATTTGAATG AAGGTGAACC CCTAGTAGCT 
CTTGGTCTTG TGGTTGGTCT AGACTATCAG AATCCATACC TGAGTCCATT TAGAGAGTTC 
CAAAGGTGGA AACACCATCC TAGCATTCGG CCAACCTTGG AAGGTGGAAA AAGGATTGCA 
TACGGAGCCA GAGCTCTCAA TGAAGGTGGC TTTCAGTCTA TACCAAAACT CACCTTTCCT 
GGTGGTTTAC TAATTGGTTG TAGTCCTGGT TTTATGAATG TTCCCAAGAT CAAAGGTACT 
CACACAGCAA TGAAAAGTGG AATTTTAGCA GCAGAATCTA TTTTTAATCA ACTAACTAGT 
GAAAATCTCC AATCAAAGAC AATAGGACTC CATGTAACTG AATATGAGGA CAATTTGAAG 
AACTCATGGG TATGGAAAGA GCTATATTCT GTTAGAAATA TAAGACCGTC CTGCCACGGA 
GTACTGGGTG TATATGGAGG GATGATTTAC ACTGGAATCT TTTACTGGAT ATTGAGAGGA 
ATGGAGCCGT GGACTCTGAA ACATAAAGGT TCTGACTTTG AACGGCTCAA GCCAGCCAAG 
GATTGCACAC CTATTGAGTA TCCAAAACCC GATGGACAGA TCAGTTTTGA CCTCTTGTCA 
TCTGTGGCTC TGAGTGGTAC TAATCATGAA CATGACCAGC CGGCACACTT AACCTTAAGG 
GATGACAGTA TACCTGTAAA TAGAAATCTG TCGATATATG ATGGGCCCGA GCAGCGATTC 
TGTCCTGCAG GAGTTTATGA ATTTGTACCT GTGGAACAAG GTGATGGATT TCGGTTACAG 
ATAAATGCTC AGAACTGTGT ACATTGTAAA ACATGTGATA TTAAAGATCC AAGTCAGAAT 
ATTAACTGGG TGGTACCTGA AGGTGGAGGA GGACCTGCTT ACAATGGAAT GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004444.2
CDS	162..2015
Translation	MLVPLAKLSCLAYQCFHALKIKKNYLPLCATRWSSTSTVPRITTHYTIYPRDKDKRWEGVNMERFAEEADVVIVGAGPAGLSAAVRLKQLAVAHEKDIRVCLVEKAAQIGAHTLSGACLDPGAFKELFPDWKEKGAPLNTPVTEDRFGILTEKYRIPVPILPGLPMNNHGNYIVRLGHLVSWMGEQAEALGVEVYPGYAAAEVLFHDDGSVKGIATNDVGIQKDGAPKATFERGLELHAKVTIFAEGCHGHLAKQLYKKFDLRANCEPQTYGIGLKELWVIDEKNWKPGRVDHTVGWPLDRHTYGGSFLYHLNEGEPLVALGLVVGLDYQNPYLSPFREFQRWKHHPSIRPTLEGGKRIAYGARALNEGGFQSIPKLTFPGGLLIGCSPGFMNVPKIKGTHTAMKSGILAAESIFNQLTSENLQSKTIGLHVTEYEDNLKNSWVWKELYSVRNIRPSCHGVLGVYGGMIYTGIFYWILRGMEPWTLKHKGSDFERLKPAKDCTPIEYPKPDGQISFDLLSSVALSGTNHEHDQPAHLTLRDDSIPVNRNLSIYDGPEQRFCPAGVYEFVPVEQGDGFRLQINAQNCVHCKTCDIKDPSQNINWVVPEGGGGPAYNGM

Target ORF information:

RefSeq Version	NM_004453.4
Organism	Homo sapiens(human)
Definition	Homo sapiens electron transfer flavoprotein dehydrogenase (ETFDH), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004453.4

ORF Insert Sequence:

ATGCTGGTGC CGCTAGCCAA GCTGTCCTGC CTGGCATATC AGTGCTTTCA TGCCTTAAAA 
ATTAAGAAAA ATTATCTACC TCTATGTGCT ACAAGATGGT CTTCAACTTC TACTGTGCCT 
CGAATTACTA CCCATTATAC TATTTATCCC CGGGATAAGG ACAAGAGATG GGAAGGAGTG 
AACATGGAAA GGTTTGCAGA AGAAGCAGAT GTTGTAATAG TTGGTGCAGG CCCTGCAGGG 
CTCTCTGCAG CTGTTCGTCT AAAACAGTTG GCTGTGGCAC ATGAAAAGGA CATCCGTGTG 
TGTCTAGTGG AGAAAGCTGC CCAGATAGGA GCTCATACTC TCTCAGGGGC TTGCCTTGAT 
CCAGGTGCTT TTAAAGAACT CTTCCCAGAC TGGAAAGAGA AGGGGGCTCC ACTTAACACT 
CCTGTAACAG AAGACAGATT TGGAATTTTA ACAGAGAAAT ACAGAATTCC TGTGCCAATT 
CTTCCAGGGC TTCCAATGAA TAATCATGGC AATTACATTG TACGCTTGGG ACATTTAGTG 
AGCTGGATGG GCGAACAAGC AGAAGCCCTT GGTGTTGAAG TATACCCTGG TTATGCAGCT 
GCTGAGGTCC TTTTTCATGA TGATGGTAGT GTAAAAGGAA TTGCCACTAA CGATGTAGGG 
ATACAAAAGG ATGGTGCACC AAAGGCAACA TTTGAGAGAG GACTGGAACT ACATGCTAAA 
GTCACAATTT TTGCAGAAGG TTGCCATGGA CATCTAGCCA AGCAACTATA TAAGAAGTTT 
GATTTGAGAG CAAATTGTGA ACCTCAAACC TACGGGATTG GACTGAAGGA GTTATGGGTT 
ATTGATGAAA AGAACTGGAA ACCTGGGAGA GTAGATCACA CTGTTGGTTG GCCCTTGGAC 
AGACATACCT ATGGAGGATC TTTCCTCTAT CATTTGAATG AAGGTGAACC CCTAGTAGCT 
CTTGGTCTTG TGGTTGGTCT AGACTATCAG AATCCATACC TGAGTCCATT TAGAGAGTTC 
CAAAGGTGGA AACACCATCC TAGCATTCGG CCAACCTTGG AAGGTGGAAA AAGGATTGCA 
TACGGAGCCA GAGCTCTCAA TGAAGGTGGC TTTCAGTCTA TACCAAAACT CACCTTTCCT 
GGTGGTTTAC TAATTGGTTG TAGTCCTGGT TTTATGAATG TTCCCAAGAT CAAAGGTACT 
CACACAGCAA TGAAAAGTGG AATTTTAGCA GCAGAATCTA TTTTTAATCA ACTAACTAGT 
GAAAATCTCC AATCAAAGAC AATAGGACTC CATGTAACTG AATATGAGGA CAATTTGAAG 
AACTCATGGG TATGGAAAGA GCTATATTCT GTTAGAAATA TAAGACCGTC CTGCCACGGA 
GTACTGGGTG TATATGGAGG GATGATTTAC ACTGGAATCT TTTACTGGAT ATTGAGAGGA 
ATGGAGCCGT GGACTCTGAA ACATAAAGGT TCTGACTTTG AACGGCTCAA GCCAGCCAAG 
GATTGCACAC CTATTGAGTA TCCAAAACCC GATGGACAGA TCAGTTTTGA CCTCTTGTCA 
TCTGTGGCTC TGAGTGGTAC TAATCATGAA CATGACCAGC CGGCACACTT AACCTTAAGG 
GATGACAGTA TACCTGTAAA TAGAAATCTG TCGATATATG ATGGGCCCGA GCAGCGATTC 
TGTCCTGCAG GAGTTTATGA ATTTGTACCT GTGGAACAAG GTGATGGATT TCGGTTACAG 
ATAAATGCTC AGAACTGTGT ACATTGTAAA ACATGTGATA TTAAAGATCC AAGTCAGAAT 
ATTAACTGGG TGGTACCTGA AGGTGGAGGA GGACCTGCTT ACAATGGAAT GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ETFDH ( NM_001281738.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001268667.1 Homo sapiens electron transfer flavoprotein dehydrogenase (ETFDH), transcript variant 3, mRNA.

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CloneID	OHu15675
Clone ID Related Accession (Same CDS sequence)	XM_024453935.1 , NM_001281738.1
Accession Version	NM_001281738.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1671bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Homo sapiens(human)
Product	electron transfer flavoprotein-ubiquinone oxidoreductase, mitochondrial isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB221748.1 and AK293737.1. Transcript Variant: This variant (3) represents use of an alternate promoter, differs in the 5' UTR, and uses a downstream start codon compared to variant 1. The resulting protein (isoform 3) is shorter and has a distinct N-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK293737.1, SRR1803611.87386.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END##

ATGGAAAGGT TTGCAGAAGA AGCAGATGTT GTAATAGTTG GTGCAGGCCC TGCAGGGCTC 
TCTGCAGCTG TTCGTCTAAA ACAGTTGGCT GTGGCACATG AAAAGGACAT CCGTGTGTGT 
CTAGTGGAGA AAGCTGCCCA GATAGGAGCT CATACTCTCT CAGGGGCTTG CCTTGATCCA 
GGTGCTTTTA AAGAACTCTT CCCAGACTGG AAAGAGAAGG GGGCTCCACT TAACACTCCT 
GTAACAGAAG ACAGATTTGG AATTTTAACA GAGAAATACA GAATTCCTGT GCCAATTCTT 
CCAGGGCTTC CAATGAATAA TCATGGCAAT TACATTGTAC GCTTGGGACA TTTAGTGAGC 
TGGATGGGCG AACAAGCAGA AGCCCTTGGT GTTGAAGTAT ACCCTGGTTA TGCAGCTGCT 
GAGGTCCTTT TTCATGATGA TGGTAGTGTA AAAGGAATTG CCACTAACGA TGTAGGGATA 
CAAAAGGATG GTGCACCAAA GGCAACATTT GAGAGAGGAC TGGAACTACA TGCTAAAGTC 
ACAATTTTTG CAGAAGGTTG CCATGGACAT CTAGCCAAGC AACTATATAA GAAGTTTGAT 
TTGAGAGCAA ATTGTGAACC TCAAACCTAC GGGATTGGAC TGAAGGAGTT ATGGGTTATT 
GATGAAAAGA ACTGGAAACC TGGGAGAGTA GATCACACTG TTGGTTGGCC CTTGGACAGA 
CATACCTATG GAGGATCTTT CCTCTATCAT TTGAATGAAG GTGAACCCCT AGTAGCTCTT 
GGTCTTGTGG TTGGTCTAGA CTATCAGAAT CCATACCTGA GTCCATTTAG AGAGTTCCAA 
AGGTGGAAAC ACCATCCTAG CATTCGGCCA ACCTTGGAAG GTGGAAAAAG GATTGCATAC 
GGAGCCAGAG CTCTCAATGA AGGTGGCTTT CAGTCTATAC CAAAACTCAC CTTTCCTGGT 
GGTTTACTAA TTGGTTGTAG TCCTGGTTTT ATGAATGTTC CCAAGATCAA AGGTACTCAC 
ACAGCAATGA AAAGTGGAAT TTTAGCAGCA GAATCTATTT TTAATCAACT AACTAGTGAA 
AATCTCCAAT CAAAGACAAT AGGACTCCAT GTAACTGAAT ATGAGGACAA TTTGAAGAAC 
TCATGGGTAT GGAAAGAGCT ATATTCTGTT AGAAATATAA GACCGTCCTG CCACGGAGTA 
CTGGGTGTAT ATGGAGGGAT GATTTACACT GGAATCTTTT ACTGGATATT GAGAGGAATG 
GAGCCGTGGA CTCTGAAACA TAAAGGTTCT GACTTTGAAC GGCTCAAGCC AGCCAAGGAT 
TGCACACCTA TTGAGTATCC AAAACCCGAT GGACAGATCA GTTTTGACCT CTTGTCATCT 
GTGGCTCTGA GTGGTACTAA TCATGAACAT GACCAGCCGG CACACTTAAC CTTAAGGGAT 
GACAGTATAC CTGTAAATAG AAATCTGTCG ATATATGATG GGCCCGAGCA GCGATTCTGT 
CCTGCAGGAG TTTATGAATT TGTACCTGTG GAACAAGGTG ATGGATTTCG GTTACAGATA 
AATGCTCAGA ACTGTGTACA TTGTAAAACA TGTGATATTA AAGATCCAAG TCAGAATATT 
AACTGGGTGG TACCTGAAGG TGGAGGAGGA CCTGCTTACA ATGGAATGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001268667.1
CDS	96..1766
Translation	MERFAEEADVVIVGAGPAGLSAAVRLKQLAVAHEKDIRVCLVEKAAQIGAHTLSGACLDPGAFKELFPDWKEKGAPLNTPVTEDRFGILTEKYRIPVPILPGLPMNNHGNYIVRLGHLVSWMGEQAEALGVEVYPGYAAAEVLFHDDGSVKGIATNDVGIQKDGAPKATFERGLELHAKVTIFAEGCHGHLAKQLYKKFDLRANCEPQTYGIGLKELWVIDEKNWKPGRVDHTVGWPLDRHTYGGSFLYHLNEGEPLVALGLVVGLDYQNPYLSPFREFQRWKHHPSIRPTLEGGKRIAYGARALNEGGFQSIPKLTFPGGLLIGCSPGFMNVPKIKGTHTAMKSGILAAESIFNQLTSENLQSKTIGLHVTEYEDNLKNSWVWKELYSVRNIRPSCHGVLGVYGGMIYTGIFYWILRGMEPWTLKHKGSDFERLKPAKDCTPIEYPKPDGQISFDLLSSVALSGTNHEHDQPAHLTLRDDSIPVNRNLSIYDGPEQRFCPAGVYEFVPVEQGDGFRLQINAQNCVHCKTCDIKDPSQNINWVVPEGGGGPAYNGM

Target ORF information:

RefSeq Version	NM_001281738.1
Organism	Homo sapiens(human)
Definition	Homo sapiens electron transfer flavoprotein dehydrogenase (ETFDH), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001281738.1

ORF Insert Sequence:

ATGGAAAGGT TTGCAGAAGA AGCAGATGTT GTAATAGTTG GTGCAGGCCC TGCAGGGCTC 
TCTGCAGCTG TTCGTCTAAA ACAGTTGGCT GTGGCACATG AAAAGGACAT CCGTGTGTGT 
CTAGTGGAGA AAGCTGCCCA GATAGGAGCT CATACTCTCT CAGGGGCTTG CCTTGATCCA 
GGTGCTTTTA AAGAACTCTT CCCAGACTGG AAAGAGAAGG GGGCTCCACT TAACACTCCT 
GTAACAGAAG ACAGATTTGG AATTTTAACA GAGAAATACA GAATTCCTGT GCCAATTCTT 
CCAGGGCTTC CAATGAATAA TCATGGCAAT TACATTGTAC GCTTGGGACA TTTAGTGAGC 
TGGATGGGCG AACAAGCAGA AGCCCTTGGT GTTGAAGTAT ACCCTGGTTA TGCAGCTGCT 
GAGGTCCTTT TTCATGATGA TGGTAGTGTA AAAGGAATTG CCACTAACGA TGTAGGGATA 
CAAAAGGATG GTGCACCAAA GGCAACATTT GAGAGAGGAC TGGAACTACA TGCTAAAGTC 
ACAATTTTTG CAGAAGGTTG CCATGGACAT CTAGCCAAGC AACTATATAA GAAGTTTGAT 
TTGAGAGCAA ATTGTGAACC TCAAACCTAC GGGATTGGAC TGAAGGAGTT ATGGGTTATT 
GATGAAAAGA ACTGGAAACC TGGGAGAGTA GATCACACTG TTGGTTGGCC CTTGGACAGA 
CATACCTATG GAGGATCTTT CCTCTATCAT TTGAATGAAG GTGAACCCCT AGTAGCTCTT 
GGTCTTGTGG TTGGTCTAGA CTATCAGAAT CCATACCTGA GTCCATTTAG AGAGTTCCAA 
AGGTGGAAAC ACCATCCTAG CATTCGGCCA ACCTTGGAAG GTGGAAAAAG GATTGCATAC 
GGAGCCAGAG CTCTCAATGA AGGTGGCTTT CAGTCTATAC CAAAACTCAC CTTTCCTGGT 
GGTTTACTAA TTGGTTGTAG TCCTGGTTTT ATGAATGTTC CCAAGATCAA AGGTACTCAC 
ACAGCAATGA AAAGTGGAAT TTTAGCAGCA GAATCTATTT TTAATCAACT AACTAGTGAA 
AATCTCCAAT CAAAGACAAT AGGACTCCAT GTAACTGAAT ATGAGGACAA TTTGAAGAAC 
TCATGGGTAT GGAAAGAGCT ATATTCTGTT AGAAATATAA GACCGTCCTG CCACGGAGTA 
CTGGGTGTAT ATGGAGGGAT GATTTACACT GGAATCTTTT ACTGGATATT GAGAGGAATG 
GAGCCGTGGA CTCTGAAACA TAAAGGTTCT GACTTTGAAC GGCTCAAGCC AGCCAAGGAT 
TGCACACCTA TTGAGTATCC AAAACCCGAT GGACAGATCA GTTTTGACCT CTTGTCATCT 
GTGGCTCTGA GTGGTACTAA TCATGAACAT GACCAGCCGG CACACTTAAC CTTAAGGGAT 
GACAGTATAC CTGTAAATAG AAATCTGTCG ATATATGATG GGCCCGAGCA GCGATTCTGT 
CCTGCAGGAG TTTATGAATT TGTACCTGTG GAACAAGGTG ATGGATTTCG GTTACAGATA 
AATGCTCAGA ACTGTGTACA TTGTAAAACA TGTGATATTA AAGATCCAAG TCAGAATATT 
AACTGGGTGG TACCTGAAGG TGGAGGAGGA CCTGCTTACA ATGGAATGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ETFDH ( XM_024453935.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024309703.1 Homo sapiens electron transfer flavoprotein dehydrogenase (ETFDH), transcript variant X1, mRNA.

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CloneID	OHu15675
Clone ID Related Accession (Same CDS sequence)	XM_024453935.1 , NM_001281738.1
Accession Version	XM_024453935.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1671bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	electron transfer flavoprotein-ubiquinone oxidoreductase, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAGGT TTGCAGAAGA AGCAGATGTT GTAATAGTTG GTGCAGGCCC TGCAGGGCTC 
TCTGCAGCTG TTCGTCTAAA ACAGTTGGCT GTGGCACATG AAAAGGACAT CCGTGTGTGT 
CTAGTGGAGA AAGCTGCCCA GATAGGAGCT CATACTCTCT CAGGGGCTTG CCTTGATCCA 
GGTGCTTTTA AAGAACTCTT CCCAGACTGG AAAGAGAAGG GGGCTCCACT TAACACTCCT 
GTAACAGAAG ACAGATTTGG AATTTTAACA GAGAAATACA GAATTCCTGT GCCAATTCTT 
CCAGGGCTTC CAATGAATAA TCATGGCAAT TACATTGTAC GCTTGGGACA TTTAGTGAGC 
TGGATGGGCG AACAAGCAGA AGCCCTTGGT GTTGAAGTAT ACCCTGGTTA TGCAGCTGCT 
GAGGTCCTTT TTCATGATGA TGGTAGTGTA AAAGGAATTG CCACTAACGA TGTAGGGATA 
CAAAAGGATG GTGCACCAAA GGCAACATTT GAGAGAGGAC TGGAACTACA TGCTAAAGTC 
ACAATTTTTG CAGAAGGTTG CCATGGACAT CTAGCCAAGC AACTATATAA GAAGTTTGAT 
TTGAGAGCAA ATTGTGAACC TCAAACCTAC GGGATTGGAC TGAAGGAGTT ATGGGTTATT 
GATGAAAAGA ACTGGAAACC TGGGAGAGTA GATCACACTG TTGGTTGGCC CTTGGACAGA 
CATACCTATG GAGGATCTTT CCTCTATCAT TTGAATGAAG GTGAACCCCT AGTAGCTCTT 
GGTCTTGTGG TTGGTCTAGA CTATCAGAAT CCATACCTGA GTCCATTTAG AGAGTTCCAA 
AGGTGGAAAC ACCATCCTAG CATTCGGCCA ACCTTGGAAG GTGGAAAAAG GATTGCATAC 
GGAGCCAGAG CTCTCAATGA AGGTGGCTTT CAGTCTATAC CAAAACTCAC CTTTCCTGGT 
GGTTTACTAA TTGGTTGTAG TCCTGGTTTT ATGAATGTTC CCAAGATCAA AGGTACTCAC 
ACAGCAATGA AAAGTGGAAT TTTAGCAGCA GAATCTATTT TTAATCAACT AACTAGTGAA 
AATCTCCAAT CAAAGACAAT AGGACTCCAT GTAACTGAAT ATGAGGACAA TTTGAAGAAC 
TCATGGGTAT GGAAAGAGCT ATATTCTGTT AGAAATATAA GACCGTCCTG CCACGGAGTA 
CTGGGTGTAT ATGGAGGGAT GATTTACACT GGAATCTTTT ACTGGATATT GAGAGGAATG 
GAGCCGTGGA CTCTGAAACA TAAAGGTTCT GACTTTGAAC GGCTCAAGCC AGCCAAGGAT 
TGCACACCTA TTGAGTATCC AAAACCCGAT GGACAGATCA GTTTTGACCT CTTGTCATCT 
GTGGCTCTGA GTGGTACTAA TCATGAACAT GACCAGCCGG CACACTTAAC CTTAAGGGAT 
GACAGTATAC CTGTAAATAG AAATCTGTCG ATATATGATG GGCCCGAGCA GCGATTCTGT 
CCTGCAGGAG TTTATGAATT TGTACCTGTG GAACAAGGTG ATGGATTTCG GTTACAGATA 
AATGCTCAGA ACTGTGTACA TTGTAAAACA TGTGATATTA AAGATCCAAG TCAGAATATT 
AACTGGGTGG TACCTGAAGG TGGAGGAGGA CCTGCTTACA ATGGAATGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024309703.1
CDS	216..1886
Translation	MERFAEEADVVIVGAGPAGLSAAVRLKQLAVAHEKDIRVCLVEKAAQIGAHTLSGACLDPGAFKELFPDWKEKGAPLNTPVTEDRFGILTEKYRIPVPILPGLPMNNHGNYIVRLGHLVSWMGEQAEALGVEVYPGYAAAEVLFHDDGSVKGIATNDVGIQKDGAPKATFERGLELHAKVTIFAEGCHGHLAKQLYKKFDLRANCEPQTYGIGLKELWVIDEKNWKPGRVDHTVGWPLDRHTYGGSFLYHLNEGEPLVALGLVVGLDYQNPYLSPFREFQRWKHHPSIRPTLEGGKRIAYGARALNEGGFQSIPKLTFPGGLLIGCSPGFMNVPKIKGTHTAMKSGILAAESIFNQLTSENLQSKTIGLHVTEYEDNLKNSWVWKELYSVRNIRPSCHGVLGVYGGMIYTGIFYWILRGMEPWTLKHKGSDFERLKPAKDCTPIEYPKPDGQISFDLLSSVALSGTNHEHDQPAHLTLRDDSIPVNRNLSIYDGPEQRFCPAGVYEFVPVEQGDGFRLQINAQNCVHCKTCDIKDPSQNINWVVPEGGGGPAYNGM

Target ORF information:

RefSeq Version	XM_024453935.1
Organism	Homo sapiens(human)
Definition	Homo sapiens electron transfer flavoprotein dehydrogenase (ETFDH), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024453935.1

ORF Insert Sequence:

ATGGAAAGGT TTGCAGAAGA AGCAGATGTT GTAATAGTTG GTGCAGGCCC TGCAGGGCTC 
TCTGCAGCTG TTCGTCTAAA ACAGTTGGCT GTGGCACATG AAAAGGACAT CCGTGTGTGT 
CTAGTGGAGA AAGCTGCCCA GATAGGAGCT CATACTCTCT CAGGGGCTTG CCTTGATCCA 
GGTGCTTTTA AAGAACTCTT CCCAGACTGG AAAGAGAAGG GGGCTCCACT TAACACTCCT 
GTAACAGAAG ACAGATTTGG AATTTTAACA GAGAAATACA GAATTCCTGT GCCAATTCTT 
CCAGGGCTTC CAATGAATAA TCATGGCAAT TACATTGTAC GCTTGGGACA TTTAGTGAGC 
TGGATGGGCG AACAAGCAGA AGCCCTTGGT GTTGAAGTAT ACCCTGGTTA TGCAGCTGCT 
GAGGTCCTTT TTCATGATGA TGGTAGTGTA AAAGGAATTG CCACTAACGA TGTAGGGATA 
CAAAAGGATG GTGCACCAAA GGCAACATTT GAGAGAGGAC TGGAACTACA TGCTAAAGTC 
ACAATTTTTG CAGAAGGTTG CCATGGACAT CTAGCCAAGC AACTATATAA GAAGTTTGAT 
TTGAGAGCAA ATTGTGAACC TCAAACCTAC GGGATTGGAC TGAAGGAGTT ATGGGTTATT 
GATGAAAAGA ACTGGAAACC TGGGAGAGTA GATCACACTG TTGGTTGGCC CTTGGACAGA 
CATACCTATG GAGGATCTTT CCTCTATCAT TTGAATGAAG GTGAACCCCT AGTAGCTCTT 
GGTCTTGTGG TTGGTCTAGA CTATCAGAAT CCATACCTGA GTCCATTTAG AGAGTTCCAA 
AGGTGGAAAC ACCATCCTAG CATTCGGCCA ACCTTGGAAG GTGGAAAAAG GATTGCATAC 
GGAGCCAGAG CTCTCAATGA AGGTGGCTTT CAGTCTATAC CAAAACTCAC CTTTCCTGGT 
GGTTTACTAA TTGGTTGTAG TCCTGGTTTT ATGAATGTTC CCAAGATCAA AGGTACTCAC 
ACAGCAATGA AAAGTGGAAT TTTAGCAGCA GAATCTATTT TTAATCAACT AACTAGTGAA 
AATCTCCAAT CAAAGACAAT AGGACTCCAT GTAACTGAAT ATGAGGACAA TTTGAAGAAC 
TCATGGGTAT GGAAAGAGCT ATATTCTGTT AGAAATATAA GACCGTCCTG CCACGGAGTA 
CTGGGTGTAT ATGGAGGGAT GATTTACACT GGAATCTTTT ACTGGATATT GAGAGGAATG 
GAGCCGTGGA CTCTGAAACA TAAAGGTTCT GACTTTGAAC GGCTCAAGCC AGCCAAGGAT 
TGCACACCTA TTGAGTATCC AAAACCCGAT GGACAGATCA GTTTTGACCT CTTGTCATCT 
GTGGCTCTGA GTGGTACTAA TCATGAACAT GACCAGCCGG CACACTTAAC CTTAAGGGAT 
GACAGTATAC CTGTAAATAG AAATCTGTCG ATATATGATG GGCCCGAGCA GCGATTCTGT 
CCTGCAGGAG TTTATGAATT TGTACCTGTG GAACAAGGTG ATGGATTTCG GTTACAGATA 
AATGCTCAGA ACTGTGTACA TTGTAAAACA TGTGATATTA AAGATCCAAG TCAGAATATT 
AACTGGGTGG TACCTGAAGG TGGAGGAGGA CCTGCTTACA ATGGAATGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ETFDH ( NM_001281737.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001268666.1 Homo sapiens electron transfer flavoprotein dehydrogenase (ETFDH), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu15884
Clone ID Related Accession (Same CDS sequence)	NM_001281737.1
Accession Version	NM_001281737.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1713bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Homo sapiens(human)
Product	electron transfer flavoprotein-ubiquinone oxidoreductase, mitochondrial isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA798232.1, AK304838.1 and AK293737.1. On Aug 31, 2013 this sequence version replaced XM_005262820.1. Transcript Variant: This variant (2) lacks an an alternate in-frame exon in the 5' coding region compared to variant 1. The resulting protein (isoform 2) is shorter but has the same N- and C-termini compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.251999.1, SRR1660809.77913.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END##

ATGCTGGTGC CGCTAGCCAA GCTGTCCTGC CTGGGAGTGA ACATGGAAAG GTTTGCAGAA 
GAAGCAGATG TTGTAATAGT TGGTGCAGGC CCTGCAGGGC TCTCTGCAGC TGTTCGTCTA 
AAACAGTTGG CTGTGGCACA TGAAAAGGAC ATCCGTGTGT GTCTAGTGGA GAAAGCTGCC 
CAGATAGGAG CTCATACTCT CTCAGGGGCT TGCCTTGATC CAGGTGCTTT TAAAGAACTC 
TTCCCAGACT GGAAAGAGAA GGGGGCTCCA CTTAACACTC CTGTAACAGA AGACAGATTT 
GGAATTTTAA CAGAGAAATA CAGAATTCCT GTGCCAATTC TTCCAGGGCT TCCAATGAAT 
AATCATGGCA ATTACATTGT ACGCTTGGGA CATTTAGTGA GCTGGATGGG CGAACAAGCA 
GAAGCCCTTG GTGTTGAAGT ATACCCTGGT TATGCAGCTG CTGAGGTCCT TTTTCATGAT 
GATGGTAGTG TAAAAGGAAT TGCCACTAAC GATGTAGGGA TACAAAAGGA TGGTGCACCA 
AAGGCAACAT TTGAGAGAGG ACTGGAACTA CATGCTAAAG TCACAATTTT TGCAGAAGGT 
TGCCATGGAC ATCTAGCCAA GCAACTATAT AAGAAGTTTG ATTTGAGAGC AAATTGTGAA 
CCTCAAACCT ACGGGATTGG ACTGAAGGAG TTATGGGTTA TTGATGAAAA GAACTGGAAA 
CCTGGGAGAG TAGATCACAC TGTTGGTTGG CCCTTGGACA GACATACCTA TGGAGGATCT 
TTCCTCTATC ATTTGAATGA AGGTGAACCC CTAGTAGCTC TTGGTCTTGT GGTTGGTCTA 
GACTATCAGA ATCCATACCT GAGTCCATTT AGAGAGTTCC AAAGGTGGAA ACACCATCCT 
AGCATTCGGC CAACCTTGGA AGGTGGAAAA AGGATTGCAT ACGGAGCCAG AGCTCTCAAT 
GAAGGTGGCT TTCAGTCTAT ACCAAAACTC ACCTTTCCTG GTGGTTTACT AATTGGTTGT 
AGTCCTGGTT TTATGAATGT TCCCAAGATC AAAGGTACTC ACACAGCAAT GAAAAGTGGA 
ATTTTAGCAG CAGAATCTAT TTTTAATCAA CTAACTAGTG AAAATCTCCA ATCAAAGACA 
ATAGGACTCC ATGTAACTGA ATATGAGGAC AATTTGAAGA ACTCATGGGT ATGGAAAGAG 
CTATATTCTG TTAGAAATAT AAGACCGTCC TGCCACGGAG TACTGGGTGT ATATGGAGGG 
ATGATTTACA CTGGAATCTT TTACTGGATA TTGAGAGGAA TGGAGCCGTG GACTCTGAAA 
CATAAAGGTT CTGACTTTGA ACGGCTCAAG CCAGCCAAGG ATTGCACACC TATTGAGTAT 
CCAAAACCCG ATGGACAGAT CAGTTTTGAC CTCTTGTCAT CTGTGGCTCT GAGTGGTACT 
AATCATGAAC ATGACCAGCC GGCACACTTA ACCTTAAGGG ATGACAGTAT ACCTGTAAAT 
AGAAATCTGT CGATATATGA TGGGCCCGAG CAGCGATTCT GTCCTGCAGG AGTTTATGAA 
TTTGTACCTG TGGAACAAGG TGATGGATTT CGGTTACAGA TAAATGCTCA GAACTGTGTA 
CATTGTAAAA CATGTGATAT TAAAGATCCA AGTCAGAATA TTAACTGGGT GGTACCTGAA 
GGTGGAGGAG GACCTGCTTA CAATGGAATG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001268666.1
CDS	357..2069
Translation	MLVPLAKLSCLGVNMERFAEEADVVIVGAGPAGLSAAVRLKQLAVAHEKDIRVCLVEKAAQIGAHTLSGACLDPGAFKELFPDWKEKGAPLNTPVTEDRFGILTEKYRIPVPILPGLPMNNHGNYIVRLGHLVSWMGEQAEALGVEVYPGYAAAEVLFHDDGSVKGIATNDVGIQKDGAPKATFERGLELHAKVTIFAEGCHGHLAKQLYKKFDLRANCEPQTYGIGLKELWVIDEKNWKPGRVDHTVGWPLDRHTYGGSFLYHLNEGEPLVALGLVVGLDYQNPYLSPFREFQRWKHHPSIRPTLEGGKRIAYGARALNEGGFQSIPKLTFPGGLLIGCSPGFMNVPKIKGTHTAMKSGILAAESIFNQLTSENLQSKTIGLHVTEYEDNLKNSWVWKELYSVRNIRPSCHGVLGVYGGMIYTGIFYWILRGMEPWTLKHKGSDFERLKPAKDCTPIEYPKPDGQISFDLLSSVALSGTNHEHDQPAHLTLRDDSIPVNRNLSIYDGPEQRFCPAGVYEFVPVEQGDGFRLQINAQNCVHCKTCDIKDPSQNINWVVPEGGGGPAYNGM

Target ORF information:

RefSeq Version	NM_001281737.1
Organism	Homo sapiens(human)
Definition	Homo sapiens electron transfer flavoprotein dehydrogenase (ETFDH), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001281737.1

ORF Insert Sequence:

ATGCTGGTGC CGCTAGCCAA GCTGTCCTGC CTGGGAGTGA ACATGGAAAG GTTTGCAGAA 
GAAGCAGATG TTGTAATAGT TGGTGCAGGC CCTGCAGGGC TCTCTGCAGC TGTTCGTCTA 
AAACAGTTGG CTGTGGCACA TGAAAAGGAC ATCCGTGTGT GTCTAGTGGA GAAAGCTGCC 
CAGATAGGAG CTCATACTCT CTCAGGGGCT TGCCTTGATC CAGGTGCTTT TAAAGAACTC 
TTCCCAGACT GGAAAGAGAA GGGGGCTCCA CTTAACACTC CTGTAACAGA AGACAGATTT 
GGAATTTTAA CAGAGAAATA CAGAATTCCT GTGCCAATTC TTCCAGGGCT TCCAATGAAT 
AATCATGGCA ATTACATTGT ACGCTTGGGA CATTTAGTGA GCTGGATGGG CGAACAAGCA 
GAAGCCCTTG GTGTTGAAGT ATACCCTGGT TATGCAGCTG CTGAGGTCCT TTTTCATGAT 
GATGGTAGTG TAAAAGGAAT TGCCACTAAC GATGTAGGGA TACAAAAGGA TGGTGCACCA 
AAGGCAACAT TTGAGAGAGG ACTGGAACTA CATGCTAAAG TCACAATTTT TGCAGAAGGT 
TGCCATGGAC ATCTAGCCAA GCAACTATAT AAGAAGTTTG ATTTGAGAGC AAATTGTGAA 
CCTCAAACCT ACGGGATTGG ACTGAAGGAG TTATGGGTTA TTGATGAAAA GAACTGGAAA 
CCTGGGAGAG TAGATCACAC TGTTGGTTGG CCCTTGGACA GACATACCTA TGGAGGATCT 
TTCCTCTATC ATTTGAATGA AGGTGAACCC CTAGTAGCTC TTGGTCTTGT GGTTGGTCTA 
GACTATCAGA ATCCATACCT GAGTCCATTT AGAGAGTTCC AAAGGTGGAA ACACCATCCT 
AGCATTCGGC CAACCTTGGA AGGTGGAAAA AGGATTGCAT ACGGAGCCAG AGCTCTCAAT 
GAAGGTGGCT TTCAGTCTAT ACCAAAACTC ACCTTTCCTG GTGGTTTACT AATTGGTTGT 
AGTCCTGGTT TTATGAATGT TCCCAAGATC AAAGGTACTC ACACAGCAAT GAAAAGTGGA 
ATTTTAGCAG CAGAATCTAT TTTTAATCAA CTAACTAGTG AAAATCTCCA ATCAAAGACA 
ATAGGACTCC ATGTAACTGA ATATGAGGAC AATTTGAAGA ACTCATGGGT ATGGAAAGAG 
CTATATTCTG TTAGAAATAT AAGACCGTCC TGCCACGGAG TACTGGGTGT ATATGGAGGG 
ATGATTTACA CTGGAATCTT TTACTGGATA TTGAGAGGAA TGGAGCCGTG GACTCTGAAA 
CATAAAGGTT CTGACTTTGA ACGGCTCAAG CCAGCCAAGG ATTGCACACC TATTGAGTAT 
CCAAAACCCG ATGGACAGAT CAGTTTTGAC CTCTTGTCAT CTGTGGCTCT GAGTGGTACT 
AATCATGAAC ATGACCAGCC GGCACACTTA ACCTTAAGGG ATGACAGTAT ACCTGTAAAT 
AGAAATCTGT CGATATATGA TGGGCCCGAG CAGCGATTCT GTCCTGCAGG AGTTTATGAA 
TTTGTACCTG TGGAACAAGG TGATGGATTT CGGTTACAGA TAAATGCTCA GAACTGTGTA 
CATTGTAAAA CATGTGATAT TAAAGATCCA AGTCAGAATA TTAACTGGGT GGTACCTGAA 
GGTGGAGGAG GACCTGCTTA CAATGGAATG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Neurite growth could be impaired by ETFDH mutation but restored by mitochondrial cofactors.
Muscle & nerve56(3)479-485(2017 Sep)
Liang WC,Lin YF,Liu TY,Chang SC,Chen BH,Nishino I,Jong YJ

[Clinical features and gene mutations in a patient with multiple aeyl-CoA dehydrogenase deficiency with severe fatty liver].
Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese journal of medical genetics33(2)191-4(2016 Apr)
Dai D,Wen F,Zhou S,Chen S

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ETFDH cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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