ETFA cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	ETFA
Entrez Gene ID	2108
Full Name	electron transfer flavoprotein alpha subunit
Synonyms	EMA,GA2,MADD
General protein information	Preferred Names electron transfer flavoprotein alpha subunit Names electron transfer flavoprotein subunit alpha, mitochondrial alpha-ETF electron transfer flavoprotein, alpha polypeptide glutaric aciduria II
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 15 Map Location: 15q24.2-q24.3
Summary	ETFA participates in catalyzing the initial step of the mitochondrial fatty acid beta-oxidation. It shuttles electrons between primary flavoprotein dehydrogenases and the membrane-bound electron transfer flavoprotein ubiquinone oxidoreductase. Defects in electron-transfer-flavoprotein have been implicated in type II glutaricaciduria in which multiple acyl-CoA dehydrogenase deficiencies result in large excretion of glutaric, lactic, ethylmalonic, butyric, isobutyric, 2-methyl-butyric, and isovaleric acids. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008].
Disorder MIM:	608053
Disorder Html:	Glutaric acidemia IIA, 231680 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_000126.4	NP_000117.1	electron transfer flavoprotein subunit alpha, mitochondrial isoform a
NM_001127716.1	NP_001121188.1	electron transfer flavoprotein subunit alpha, mitochondrial isoform b
NM_000126.3	NP_000117.1	electron transfer flavoprotein subunit alpha, mitochondrial isoform a

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Etfa	NP_663590.3
Danio rerio (zebrafish)	etfa	NP_944591.1
Caenorhabditis elegans (roundworm)	CELE_F27D4.1	NP_492146.1
Schizosaccharomyces pombe (fission yeast)	SPAC27D7.06	NP_594612.1
Arabidopsis thaliana (thale cress)	ETFALPHA	NP_175507.1
Homo sapiens (human)	ETFA	NP_000117.1
	ETFA	XP_001104838.1
Eremothecium gossypii	AGOS_AGL054W	NP_986612.1
Oryza sativa (rice)	Os03g0835400	NP_001051818.1
Canis lupus familiaris (dog)	ETFA	XP_853387.1
Rattus norvegicus (Norway rat)	Etfa	NP_001009668.1
Drosophila melanogaster (fruit fly)	wal	NP_476975.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004031	XP_318487.4
Kluyveromyces lactis	KLLA0E14037g	XP_454584.1
Neurospora crassa	NCU08004	XP_962674.2
Magnaporthe oryzae (rice blast fungus)	MGG_01719	XP_003714716.1
Xenopus tropicalis (tropical clawed frog)	etfa	NP_001016738.1
Pan troglodytes (chimpanzee)	ETFA	XP_510690.2
Bos taurus (cattle)	ETFA	NP_001069290.1
Gallus gallus (chicken)	ETFA	NP_001025714.1
Saccharomyces cerevisiae (baker's yeast)	AIM45	NP_015329.1

Gene Ontology
Bibliography

Related articles in PubMed

Oxidation of the FAD cofactor to the 8-formyl-derivative in human electron transferring flavoprotein.
Augustin P, Toplak M, Fuchs K, Gerstmann EC, Prassl R, Winkler A, Macheroux P
The Journal of biological chemistry(2018 Jan)

A Single Adaptable Cochaperone-Scaffold Complex Delivers Nascent Iron-Sulfur Clusters to Mammalian Respiratory Chain Complexes I-III.
Maio N, Kim KS, Singh A, Rouault TA
Cell metabolism25(4)945-953.e6(2017 Apr)

Additional evidence supports association of common genetic variants in VTI1A and ETFA with increased risk of glioma susceptibility.
Wang N, Deng Z, Wang M, Li R, Xu G, Bao G
Journal of the neurological sciences375282-288(2017 Apr)

Mitochondrial Protein Interaction Mapping Identifies Regulators of Respiratory Chain Function.
Floyd BJ, Wilkerson EM, Veling MT, Minogue CE, Xia C, Beebe ET, Wrobel RL, Cho H, Kremer LS, Alston CL, Gromek KA, Dolan BK, Ulbrich A, Stefely JA, Bohl SL, Werner KM, Jochem A, Westphall MS, Rensvold JW, Taylor RW, Prokisch H, Kim JP, Coon JJ, Pagliarini DJ
Molecular cell63(4)621-632(2016 Aug)

Widespread Expansion of Protein Interaction Capabilities by Alternative Splicing.
Yang X, Coulombe-Huntington J, Kang S, Sheynkman GM, Hao T, Richardson A, Sun S, Yang F, Shen YA, Murray RR, Spirohn K, Begg BE, Duran-Frigola M, MacWilliams A, Pevzner SJ, Zhong Q, Trigg SA, Tam S, Ghamsari L, Sahni N, Yi S, Rodriguez MD, Balcha D, Tan G, Costanzo M, Andrews B, Boone C, Zhou XJ, Salehi-Ashtiani K, Charloteaux B, Chen AA, Calderwood MA, Aloy P, Roth FP, Hill DE, Iakoucheva LM, Xia Y, Vidal M
Cell164(4)805-17(2016 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

our results indicate that genetic variants in ETFA may modify individual susceptibility to non-GBM of glioma in the Han Chinese population and support the role of the ETFA genes in the occurrence of glioma.
Title: Additional evidence supports association of common genetic variants in VTI1A and ETFA with increased risk of glioma susceptibility.

the mechanism of tert-butyl hydroperoxide-induced an apoptosis cascade and endoplasmic reticulum stress in hepatocyte cells by up-regulation of ETFA, providing a new mechanism for liver injury.
Title: Exploring the effects of tert-butylhydroperoxide induced liver injury using proteomic approach.

These results are consistent with the electron transfer flavoprotein alpha II domain adopting orientations in solution that deviate from the crystal structure of free ETF towards the active, substrate-bound orientation.
Title: Electron transfer flavoprotein domain II orientation monitored using double electron-electron resonance between an enzymatically reduced, native FAD cofactor, and spin labels.

investigations are compatible with the notion that the ETFalpha-T171 variant displays an altered conformational landscape that results in reduced protein function under thermal stress
Title: A polymorphic position in electron transfer flavoprotein modulates kinetic stability as evidenced by thermal stress.

Observational study of gene-disease association. (HuGE Navigator)
Title: Genetic variants in nuclear-encoded mitochondrial genes influence AIDS progression.

The following ETFA gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ETFA cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu18595	NM_000126.4 Latest version!	Homo sapiens electron transfer flavoprotein subunit alpha (ETFA), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
OHu16532	NM_001127716.1 Latest version!	Homo sapiens electron transfer flavoprotein subunit alpha (ETFA), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
View Old Accession Versions >>
OHu18595	NM_000126.3	Homo sapiens electron transfer flavoprotein alpha subunit (ETFA), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ETFA ( NM_000126.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_000117.1 Homo sapiens electron transfer flavoprotein subunit alpha (ETFA), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu18595
Clone ID Related Accession (Same CDS sequence)	NM_000126.4 , NM_000126.3
Accession Version	NM_000126.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1002bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-21
Organism	Homo sapiens(human)
Product	electron transfer flavoprotein subunit alpha, mitochondrial isoform a
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK292979.1, AA557337.1, BG261160.1 and AC091100.14. This sequence is a reference standard in the RefSeqGene project. On Nov 22, 2018 this sequence version replaced NM_000126.3. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK292979.1, SRR1803614.62452.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2153946, SAMEA2155550 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta MANE Ensembl match :: ENST00000557943.6/ ENSP00000452762.1 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGTTCCGAG CGGCGGCTCC GGGGCAGCTC CGGCGGGCGG CCTCATTGCT ACGATTTCAG 
AGTACCCTGG TAATAGCTGA GCATGCAAAT GATTCCCTAG CACCCATTAC TTTAAATACC 
ATTACTGCAG CCACACGCCT TGGAGGTGAA GTGTCCTGCT TAGTAGCTGG AACCAAATGT 
GACAAGGTGG CACAAGATCT CTGTAAAGTA GCAGGCATAG CAAAAGTTCT GGTGGCTCAG 
CATGATGTGT ACAAAGGCCT ACTTCCAGAG GAACTGACAC CATTGATTTT GGCAACTCAG 
AAGCAGTTCA ATTACACACA CATCTGTGCT GGAGCATCTG CCTTCGGAAA GAACCTTTTG 
CCCAGAGTAG CAGCCAAACT TGAGGTTGCC CCGATTTCTG ACATCATTGC AATCAAGTCA 
CCTGACACAT TTGTGAGAAC TATTTATGCA GGAAATGCTC TATGTACAGT GAAGTGTGAT 
GAGAAAGTGA AAGTGTTTTC TGTCCGTGGA ACATCCTTTG ATGCTGCAGC AACAAGTGGC 
GGTAGTGCCA GTTCAGAAAA GGCATCAAGT ACTTCACCAG TGGAAATATC AGAGTGGCTT 
GACCAGAAAT TAACAAAAAG TGATCGACCA GAGCTAACAG GTGCCAAAGT GGTGGTATCT 
GGTGGTCGAG GCTTGAAGAG TGGAGAGAAC TTTAAGTTGT TATATGACTT GGCAGATCAA 
CTACATGCTG CAGTTGGTGC TTCCCGTGCT GCTGTTGATG CTGGCTTTGT TCCCAATGAC 
ATGCAAGTTG GACAGACGGG AAAAATAGTA GCACCAGAAC TTTATATTGC TGTTGGAATA 
TCTGGAGCCA TCCAACATTT AGCTGGGATG AAAGACAGCA AGACAATTGT GGCAATTAAT 
AAAGACCCAG AAGCTCCAAT TTTCCAAGTG GCAGATTATG GAATAGTTGC AGATTTATTT 
AAGGTAGTTC CTGAAATGAC TGAGATATTG AAGAAAAAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000117.1
CDS	82..1083
Translation	MFRAAAPGQLRRAASLLRFQSTLVIAEHANDSLAPITLNTITAATRLGGEVSCLVAGTKCDKVAQDLCKVAGIAKVLVAQHDVYKGLLPEELTPLILATQKQFNYTHICAGASAFGKNLLPRVAAKLEVAPISDIIAIKSPDTFVRTIYAGNALCTVKCDEKVKVFSVRGTSFDAAATSGGSASSEKASSTSPVEISEWLDQKLTKSDRPELTGAKVVVSGGRGLKSGENFKLLYDLADQLHAAVGASRAAVDAGFVPNDMQVGQTGKIVAPELYIAVGISGAIQHLAGMKDSKTIVAINKDPEAPIFQVADYGIVADLFKVVPEMTEILKKK

Target ORF information:

RefSeq Version	NM_000126.4
Organism	Homo sapiens(human)
Definition	Homo sapiens electron transfer flavoprotein subunit alpha (ETFA), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000126.4

ORF Insert Sequence:

ATGTTCCGAG CGGCGGCTCC GGGGCAGCTC CGGCGGGCGG CCTCATTGCT ACGATTTCAG 
AGTACCCTGG TAATAGCTGA GCATGCAAAT GATTCCCTAG CACCCATTAC TTTAAATACC 
ATTACTGCAG CCACACGCCT TGGAGGTGAA GTGTCCTGCT TAGTAGCTGG AACCAAATGT 
GACAAGGTGG CACAAGATCT CTGTAAAGTA GCAGGCATAG CAAAAGTTCT GGTGGCTCAG 
CATGATGTGT ACAAAGGCCT ACTTCCAGAG GAACTGACAC CATTGATTTT GGCAACTCAG 
AAGCAGTTCA ATTACACACA CATCTGTGCT GGAGCATCTG CCTTCGGAAA GAACCTTTTG 
CCCAGAGTAG CAGCCAAACT TGAGGTTGCC CCGATTTCTG ACATCATTGC AATCAAGTCA 
CCTGACACAT TTGTGAGAAC TATTTATGCA GGAAATGCTC TATGTACAGT GAAGTGTGAT 
GAGAAAGTGA AAGTGTTTTC TGTCCGTGGA ACATCCTTTG ATGCTGCAGC AACAAGTGGC 
GGTAGTGCCA GTTCAGAAAA GGCATCAAGT ACTTCACCAG TGGAAATATC AGAGTGGCTT 
GACCAGAAAT TAACAAAAAG TGATCGACCA GAGCTAACAG GTGCCAAAGT GGTGGTATCT 
GGTGGTCGAG GCTTGAAGAG TGGAGAGAAC TTTAAGTTGT TATATGACTT GGCAGATCAA 
CTACATGCTG CAGTTGGTGC TTCCCGTGCT GCTGTTGATG CTGGCTTTGT TCCCAATGAC 
ATGCAAGTTG GACAGACGGG AAAAATAGTA GCACCAGAAC TTTATATTGC TGTTGGAATA 
TCTGGAGCCA TCCAACATTT AGCTGGGATG AAAGACAGCA AGACAATTGT GGCAATTAAT 
AAAGACCCAG AAGCTCCAAT TTTCCAAGTG GCAGATTATG GAATAGTTGC AGATTTATTT 
AAGGTAGTTC CTGAAATGAC TGAGATATTG AAGAAAAAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ETFA ( NM_000126.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_000117.1 Homo sapiens electron transfer flavoprotein alpha subunit (ETFA), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu18595
Clone ID Related Accession (Same CDS sequence)	NM_000126.4 , NM_000126.3
Accession Version	NM_000126.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1002bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-26
Organism	Homo sapiens(human)
Product	electron transfer flavoprotein subunit alpha, mitochondrial isoform a
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK292979.1, AA557337.1 and BG261160.1. This sequence is a reference standard in the RefSeqGene project. On May 30, 2008 this sequence version replaced NM_000126.2. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK292979.1, BC029995.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2153946, SAMEA2155550 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGTTCCGAG CGGCGGCTCC GGGGCAGCTC CGGCGGGCGG CCTCATTGCT ACGATTTCAG 
AGTACCCTGG TAATAGCTGA GCATGCAAAT GATTCCCTAG CACCCATTAC TTTAAATACC 
ATTACTGCAG CCACACGCCT TGGAGGTGAA GTGTCCTGCT TAGTAGCTGG AACCAAATGT 
GACAAGGTGG CACAAGATCT CTGTAAAGTA GCAGGCATAG CAAAAGTTCT GGTGGCTCAG 
CATGATGTGT ACAAAGGCCT ACTTCCAGAG GAACTGACAC CATTGATTTT GGCAACTCAG 
AAGCAGTTCA ATTACACACA CATCTGTGCT GGAGCATCTG CCTTCGGAAA GAACCTTTTG 
CCCAGAGTAG CAGCCAAACT TGAGGTTGCC CCGATTTCTG ACATCATTGC AATCAAGTCA 
CCTGACACAT TTGTGAGAAC TATTTATGCA GGAAATGCTC TATGTACAGT GAAGTGTGAT 
GAGAAAGTGA AAGTGTTTTC TGTCCGTGGA ACATCCTTTG ATGCTGCAGC AACAAGTGGC 
GGTAGTGCCA GTTCAGAAAA GGCATCAAGT ACTTCACCAG TGGAAATATC AGAGTGGCTT 
GACCAGAAAT TAACAAAAAG TGATCGACCA GAGCTAACAG GTGCCAAAGT GGTGGTATCT 
GGTGGTCGAG GCTTGAAGAG TGGAGAGAAC TTTAAGTTGT TATATGACTT GGCAGATCAA 
CTACATGCTG CAGTTGGTGC TTCCCGTGCT GCTGTTGATG CTGGCTTTGT TCCCAATGAC 
ATGCAAGTTG GACAGACGGG AAAAATAGTA GCACCAGAAC TTTATATTGC TGTTGGAATA 
TCTGGAGCCA TCCAACATTT AGCTGGGATG AAAGACAGCA AGACAATTGT GGCAATTAAT 
AAAGACCCAG AAGCTCCAAT TTTCCAAGTG GCAGATTATG GAATAGTTGC AGATTTATTT 
AAGGTAGTTC CTGAAATGAC TGAGATATTG AAGAAAAAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000117.1
CDS	82..1083
Translation	MFRAAAPGQLRRAASLLRFQSTLVIAEHANDSLAPITLNTITAATRLGGEVSCLVAGTKCDKVAQDLCKVAGIAKVLVAQHDVYKGLLPEELTPLILATQKQFNYTHICAGASAFGKNLLPRVAAKLEVAPISDIIAIKSPDTFVRTIYAGNALCTVKCDEKVKVFSVRGTSFDAAATSGGSASSEKASSTSPVEISEWLDQKLTKSDRPELTGAKVVVSGGRGLKSGENFKLLYDLADQLHAAVGASRAAVDAGFVPNDMQVGQTGKIVAPELYIAVGISGAIQHLAGMKDSKTIVAINKDPEAPIFQVADYGIVADLFKVVPEMTEILKKK

Target ORF information:

RefSeq Version	NM_000126.3
Organism	Homo sapiens(human)
Definition	Homo sapiens electron transfer flavoprotein alpha subunit (ETFA), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000126.3

ORF Insert Sequence:

ATGTTCCGAG CGGCGGCTCC GGGGCAGCTC CGGCGGGCGG CCTCATTGCT ACGATTTCAG 
AGTACCCTGG TAATAGCTGA GCATGCAAAT GATTCCCTAG CACCCATTAC TTTAAATACC 
ATTACTGCAG CCACACGCCT TGGAGGTGAA GTGTCCTGCT TAGTAGCTGG AACCAAATGT 
GACAAGGTGG CACAAGATCT CTGTAAAGTA GCAGGCATAG CAAAAGTTCT GGTGGCTCAG 
CATGATGTGT ACAAAGGCCT ACTTCCAGAG GAACTGACAC CATTGATTTT GGCAACTCAG 
AAGCAGTTCA ATTACACACA CATCTGTGCT GGAGCATCTG CCTTCGGAAA GAACCTTTTG 
CCCAGAGTAG CAGCCAAACT TGAGGTTGCC CCGATTTCTG ACATCATTGC AATCAAGTCA 
CCTGACACAT TTGTGAGAAC TATTTATGCA GGAAATGCTC TATGTACAGT GAAGTGTGAT 
GAGAAAGTGA AAGTGTTTTC TGTCCGTGGA ACATCCTTTG ATGCTGCAGC AACAAGTGGC 
GGTAGTGCCA GTTCAGAAAA GGCATCAAGT ACTTCACCAG TGGAAATATC AGAGTGGCTT 
GACCAGAAAT TAACAAAAAG TGATCGACCA GAGCTAACAG GTGCCAAAGT GGTGGTATCT 
GGTGGTCGAG GCTTGAAGAG TGGAGAGAAC TTTAAGTTGT TATATGACTT GGCAGATCAA 
CTACATGCTG CAGTTGGTGC TTCCCGTGCT GCTGTTGATG CTGGCTTTGT TCCCAATGAC 
ATGCAAGTTG GACAGACGGG AAAAATAGTA GCACCAGAAC TTTATATTGC TGTTGGAATA 
TCTGGAGCCA TCCAACATTT AGCTGGGATG AAAGACAGCA AGACAATTGT GGCAATTAAT 
AAAGACCCAG AAGCTCCAAT TTTCCAAGTG GCAGATTATG GAATAGTTGC AGATTTATTT 
AAGGTAGTTC CTGAAATGAC TGAGATATTG AAGAAAAAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ETFA ( NM_001127716.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001121188.1 Homo sapiens electron transfer flavoprotein subunit alpha (ETFA), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu16532
Clone ID Related Accession (Same CDS sequence)	NM_001127716.1
Accession Version	NM_001127716.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 855bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-03
Organism	Homo sapiens(human)
Product	electron transfer flavoprotein subunit alpha, mitochondrial isoform b
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK292979.1, BU176141.1, AA557337.1 and BG261160.1. Transcript Variant: This variant (2) lacks an alternate in-frame exon compared to variant 1. The resulting isoform (b) has the same N- and C-termini but is shorter compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.189828.1, SRR1163655.656373.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2153946, SAMEA2155550 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGTTCCGAG CGGCGGCTCC GGGGCAGCTC CGGCGGGCGG TGGCACAAGA TCTCTGTAAA 
GTAGCAGGCA TAGCAAAAGT TCTGGTGGCT CAGCATGATG TGTACAAAGG CCTACTTCCA 
GAGGAACTGA CACCATTGAT TTTGGCAACT CAGAAGCAGT TCAATTACAC ACACATCTGT 
GCTGGAGCAT CTGCCTTCGG AAAGAACCTT TTGCCCAGAG TAGCAGCCAA ACTTGAGGTT 
GCCCCGATTT CTGACATCAT TGCAATCAAG TCACCTGACA CATTTGTGAG AACTATTTAT 
GCAGGAAATG CTCTATGTAC AGTGAAGTGT GATGAGAAAG TGAAAGTGTT TTCTGTCCGT 
GGAACATCCT TTGATGCTGC AGCAACAAGT GGCGGTAGTG CCAGTTCAGA AAAGGCATCA 
AGTACTTCAC CAGTGGAAAT ATCAGAGTGG CTTGACCAGA AATTAACAAA AAGTGATCGA 
CCAGAGCTAA CAGGTGCCAA AGTGGTGGTA TCTGGTGGTC GAGGCTTGAA GAGTGGAGAG 
AACTTTAAGT TGTTATATGA CTTGGCAGAT CAACTACATG CTGCAGTTGG TGCTTCCCGT 
GCTGCTGTTG ATGCTGGCTT TGTTCCCAAT GACATGCAAG TTGGACAGAC GGGAAAAATA 
GTAGCACCAG AACTTTATAT TGCTGTTGGA ATATCTGGAG CCATCCAACA TTTAGCTGGG 
ATGAAAGACA GCAAGACAAT TGTGGCAATT AATAAAGACC CAGAAGCTCC AATTTTCCAA 
GTGGCAGATT ATGGAATAGT TGCAGATTTA TTTAAGGTAG TTCCTGAAAT GACTGAGATA 
TTGAAGAAAA AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001121188.1
CDS	82..936
Translation	MFRAAAPGQLRRAVAQDLCKVAGIAKVLVAQHDVYKGLLPEELTPLILATQKQFNYTHICAGASAFGKNLLPRVAAKLEVAPISDIIAIKSPDTFVRTIYAGNALCTVKCDEKVKVFSVRGTSFDAAATSGGSASSEKASSTSPVEISEWLDQKLTKSDRPELTGAKVVVSGGRGLKSGENFKLLYDLADQLHAAVGASRAAVDAGFVPNDMQVGQTGKIVAPELYIAVGISGAIQHLAGMKDSKTIVAINKDPEAPIFQVADYGIVADLFKVVPEMTEILKKK

Target ORF information:

RefSeq Version	NM_001127716.1
Organism	Homo sapiens(human)
Definition	Homo sapiens electron transfer flavoprotein subunit alpha (ETFA), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001127716.1

ORF Insert Sequence:

ATGTTCCGAG CGGCGGCTCC GGGGCAGCTC CGGCGGGCGG TGGCACAAGA TCTCTGTAAA 
GTAGCAGGCA TAGCAAAAGT TCTGGTGGCT CAGCATGATG TGTACAAAGG CCTACTTCCA 
GAGGAACTGA CACCATTGAT TTTGGCAACT CAGAAGCAGT TCAATTACAC ACACATCTGT 
GCTGGAGCAT CTGCCTTCGG AAAGAACCTT TTGCCCAGAG TAGCAGCCAA ACTTGAGGTT 
GCCCCGATTT CTGACATCAT TGCAATCAAG TCACCTGACA CATTTGTGAG AACTATTTAT 
GCAGGAAATG CTCTATGTAC AGTGAAGTGT GATGAGAAAG TGAAAGTGTT TTCTGTCCGT 
GGAACATCCT TTGATGCTGC AGCAACAAGT GGCGGTAGTG CCAGTTCAGA AAAGGCATCA 
AGTACTTCAC CAGTGGAAAT ATCAGAGTGG CTTGACCAGA AATTAACAAA AAGTGATCGA 
CCAGAGCTAA CAGGTGCCAA AGTGGTGGTA TCTGGTGGTC GAGGCTTGAA GAGTGGAGAG 
AACTTTAAGT TGTTATATGA CTTGGCAGAT CAACTACATG CTGCAGTTGG TGCTTCCCGT 
GCTGCTGTTG ATGCTGGCTT TGTTCCCAAT GACATGCAAG TTGGACAGAC GGGAAAAATA 
GTAGCACCAG AACTTTATAT TGCTGTTGGA ATATCTGGAG CCATCCAACA TTTAGCTGGG 
ATGAAAGACA GCAAGACAAT TGTGGCAATT AATAAAGACC CAGAAGCTCC AATTTTCCAA 
GTGGCAGATT ATGGAATAGT TGCAGATTTA TTTAAGGTAG TTCCTGAAAT GACTGAGATA 
TTGAAGAAAA AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Oxidation of the FAD cofactor to the 8-formyl-derivative in human electron transferring flavoprotein.
The Journal of biological chemistry(2018 Jan)
Augustin P,Toplak M,Fuchs K,Gerstmann EC,Prassl R,Winkler A,Macheroux P

A Single Adaptable Cochaperone-Scaffold Complex Delivers Nascent Iron-Sulfur Clusters to Mammalian Respiratory Chain Complexes I-III.
Cell metabolism25(4)945-953.e6(2017 Apr)
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Additional evidence supports association of common genetic variants in VTI1A and ETFA with increased risk of glioma susceptibility.
Journal of the neurological sciences375282-288(2017 Apr)
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Mitochondrial Protein Interaction Mapping Identifies Regulators of Respiratory Chain Function.
Molecular cell63(4)621-632(2016 Aug)
Floyd BJ,Wilkerson EM,Veling MT,Minogue CE,Xia C,Beebe ET,Wrobel RL,Cho H,Kremer LS,Alston CL,Gromek KA,Dolan BK,Ulbrich A,Stefely JA,Bohl SL,Werner KM,Jochem A,Westphall MS,Rensvold JW,Taylor RW,Prokisch H,Kim JP,Coon JJ,Pagliarini DJ

Widespread Expansion of Protein Interaction Capabilities by Alternative Splicing.
Cell164(4)805-17(2016 Feb)
Yang X,Coulombe-Huntington J,Kang S,Sheynkman GM,Hao T,Richardson A,Sun S,Yang F,Shen YA,Murray RR,Spirohn K,Begg BE,Duran-Frigola M,MacWilliams A,Pevzner SJ,Zhong Q,Trigg SA,Tam S,Ghamsari L,Sahni N,Yi S,Rodriguez MD,Balcha D,Tan G,Costanzo M,Andrews B,Boone C,Zhou XJ,Salehi-Ashtiani K,Charloteaux B,Chen AA,Calderwood MA,Aloy P,Roth FP,Hill DE,Iakoucheva LM,Xia Y,Vidal M

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ETFA cDNA ORF clone, Homo sapiens(human)

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ORF Insert Sequence:

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ORF Insert Sequence:

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ORF Insert Sequence:

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