ERBB2 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	ERBB2
Entrez Gene ID	2064
Full Name	erb-b2 receptor tyrosine kinase 2
Synonyms	CD340,HER-2,HER-2/neu,HER2,MLN 19,NEU,NGL,TKR1
General protein information	Preferred Names erb-b2 receptor tyrosine kinase 2 Names receptor tyrosine-protein kinase erbB-2 c-erb B2/neu protein herstatin human epidermal growth factor receptor 2 metastatic lymph node gene 19 protein neuro/glioblastoma derived oncogene homolog neuroblastoma/glioblastoma derived oncogene homolog p185erbB2 proto-oncogene Neu proto-oncogene c-ErbB-2 tyrosine kinase-type cell surface receptor HER2 v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2 v-erb-b2 avian erythroblastic leukemia viral oncoprotein 2 v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 17 Map Location: 17q12
Summary	This gene encodes a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. This protein has no ligand binding domain of its own and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. Allelic variations at amino acid positions 654 and 655 of isoform a (positions 624 and 625 of isoform b) have been reported, with the most common allele, Ile654/Ile655, shown here. Amplification and/or overexpression of this gene has been reported in numerous cancers, including breast and ovarian tumors. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterized. [provided by RefSeq, Jul 2008].
Disorder MIM:	164870
Disorder Html:	Adenocarcinoma of lung, somatic, 211980 (3); Glioblastoma, somatic, 137800 (3); Gastric cancer, somatic, 613659 (3); Ovarian cancer, somatic, (3)

mRNA and Protein(s)

mRNA	Protein	Name
XM_024450643.1	XP_024306411.1	receptor tyrosine-protein kinase erbB-2 isoform X3
NM_001289938.1	NP_001276867.1	receptor tyrosine-protein kinase erbB-2 isoform e
NM_001289936.1	NP_001276865.1	receptor tyrosine-protein kinase erbB-2 isoform c
NM_001005862.2	NP_001005862.1	receptor tyrosine-protein kinase erbB-2 isoform b
XM_024450642.1	XP_024306410.1	receptor tyrosine-protein kinase erbB-2 isoform X2
NM_004448.3	NP_004439.2	receptor tyrosine-protein kinase erbB-2 isoform a precursor
XM_024450641.1	XP_024306409.1	receptor tyrosine-protein kinase erbB-2 isoform X1
NM_001289937.1	NP_001276866.1	receptor tyrosine-protein kinase erbB-2 isoform d precursor

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	ERBB2	XP_003315512.2
Mus musculus (house mouse)	Erbb2	NP_001003817.1
	ERBB2	XP_001090430.1
Rattus norvegicus (Norway rat)	Erbb2	NP_058699.2
Danio rerio (zebrafish)	erbb2	NP_956413.2
Homo sapiens (human)	ERBB2	NP_004439.2
Canis lupus familiaris (dog)	ERBB2	NP_001003217.1
Gallus gallus (chicken)	ERBB2	NP_001038126.1
Bos taurus (cattle)	ERBB2	XP_003587474.1
Xenopus tropicalis (tropical clawed frog)	erbb2	XP_002940420.2

Gene Ontology
Bibliography

Related articles in PubMed

Translational control by an upstream open reading frame in the HER-2/neu transcript.
Child SJ, Miller MK, Geballe AP
The Journal of biological chemistry274(34)24335-41(1999 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

HER2 transcription levels were found to be significantly related to pT class, pN class, and tumor recurrence. mRNA expression was well correlated with protein overexpression and gene amplification
Title: In situ analysis of HER2 mRNA in gastric carcinoma: comparison with fluorescence in situ hybridization, dual-color silver in situ hybridization, and immunohistochemistry.

ErbB2 levels inversely impact cell clathrin-mediated endocytosis capacity in breast cancer cells treated with geldanamycin.
Title: The HSP90 inhibitor geldanamycin perturbs endosomal structure and drives recycling ErbB2 and transferrin to modified MVBs/lysosomal compartments.

ERBB1 and ERBB2 have different roles in response to antineoplastic agents in colorectal cancer cells
Title: Direct and immune mediated antibody targeting of ERBB receptors in a colorectal cancer cell-line panel.

Twenty-seven point five percent of oesophageal adenocarcinomas showed evidence of HER2 over expression.
Title: Human epidermal growth factor receptor-2 in oesophageal cancers: an observational study.

Primary human ovarian epithelial cancer cells broadly express HER2 at immunologically-detectable levels.
Title: Primary human ovarian epithelial cancer cells broadly express HER2 at immunologically-detectable levels.

The following ERBB2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ERBB2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu23956	NM_001289936.1 Latest version!	Homo sapiens erb-b2 receptor tyrosine kinase 2 (ERBB2), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$459.50-$643.30 $919.00
OHu27580	NM_001005862.2 Latest version!	Homo sapiens erb-b2 receptor tyrosine kinase 2 (ERBB2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$619.50-$867.30 $1239.00
OHu27656	NM_004448.3 Latest version!	Homo sapiens erb-b2 receptor tyrosine kinase 2 (ERBB2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$634.50-$888.30 $1269.00
OHu27526	NM_001289938.1 Latest version!	Homo sapiens erb-b2 receptor tyrosine kinase 2 (ERBB2), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00
OHu104276	XM_024450641.1 Latest version!	Homo sapiens erb-b2 receptor tyrosine kinase 2 (ERBB2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OHu104277	XM_024450642.1 Latest version!	Homo sapiens erb-b2 receptor tyrosine kinase 2 (ERBB2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OHu104278	XM_024450643.1 Latest version!	Homo sapiens erb-b2 receptor tyrosine kinase 2 (ERBB2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OHu24035	NM_001289937.1 Latest version!	Homo sapiens erb-b2 receptor tyrosine kinase 2 (ERBB2), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ERBB2 ( NM_001289936.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001276865.1 Homo sapiens erb-b2 receptor tyrosine kinase 2 (ERBB2), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu23956
Clone ID Related Accession (Same CDS sequence)	NM_001289936.1
Accession Version	NM_001289936.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3723bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-03
Organism	Homo sapiens(human)
Product	receptor tyrosine-protein kinase erbB-2 isoform c
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK295195.1, BC080193.1 and BM678576.1. This sequence is a reference standard in the RefSeqGene project. On Feb 11, 2014 this sequence version replaced XM_006721766.1. Transcript Variant: This variant (3) has an alternate 5' UTR and 5' coding region, compared to variant 1. The resulting isoform (c) has a distinct and shorter N-terminus, compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK295195.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END##

ATGCCCCGGG GGTCCTGGAA GCCACAAGTG TGCACCGGCA CAGACATGAA GCTGCGGCTC 
CCTGCCAGTC CCGAGACCCA CCTGGACATG CTCCGCCACC TCTACCAGGG CTGCCAGGTG 
GTGCAGGGAA ACCTGGAACT CACCTACCTG CCCACCAATG CCAGCCTGTC CTTCCTGCAG 
GATATCCAGG AGGTGCAGGG CTACGTGCTC ATCGCTCACA ACCAAGTGAG GCAGGTCCCA 
CTGCAGAGGC TGCGGATTGT GCGAGGCACC CAGCTCTTTG AGGACAACTA TGCCCTGGCC 
GTGCTAGACA ATGGAGACCC GCTGAACAAT ACCACCCCTG TCACAGGGGC CTCCCCAGGA 
GGCCTGCGGG AGCTGCAGCT TCGAAGCCTC ACAGAGATCT TGAAAGGAGG GGTCTTGATC 
CAGCGGAACC CCCAGCTCTG CTACCAGGAC ACGATTTTGT GGAAGGACAT CTTCCACAAG 
AACAACCAGC TGGCTCTCAC ACTGATAGAC ACCAACCGCT CTCGGGCCTG CCACCCCTGT 
TCTCCGATGT GTAAGGGCTC CCGCTGCTGG GGAGAGAGTT CTGAGGATTG TCAGAGCCTG 
ACGCGCACTG TCTGTGCCGG TGGCTGTGCC CGCTGCAAGG GGCCACTGCC CACTGACTGC 
TGCCATGAGC AGTGTGCTGC CGGCTGCACG GGCCCCAAGC ACTCTGACTG CCTGGCCTGC 
CTCCACTTCA ACCACAGTGG CATCTGTGAG CTGCACTGCC CAGCCCTGGT CACCTACAAC 
ACAGACACGT TTGAGTCCAT GCCCAATCCC GAGGGCCGGT ATACATTCGG CGCCAGCTGT 
GTGACTGCCT GTCCCTACAA CTACCTTTCT ACGGACGTGG GATCCTGCAC CCTCGTCTGC 
CCCCTGCACA ACCAAGAGGT GACAGCAGAG GATGGAACAC AGCGGTGTGA GAAGTGCAGC 
AAGCCCTGTG CCCGAGTGTG CTATGGTCTG GGCATGGAGC ACTTGCGAGA GGTGAGGGCA 
GTTACCAGTG CCAATATCCA GGAGTTTGCT GGCTGCAAGA AGATCTTTGG GAGCCTGGCA 
TTTCTGCCGG AGAGCTTTGA TGGGGACCCA GCCTCCAACA CTGCCCCGCT CCAGCCAGAG 
CAGCTCCAAG TGTTTGAGAC TCTGGAAGAG ATCACAGGTT ACCTATACAT CTCAGCATGG 
CCGGACAGCC TGCCTGACCT CAGCGTCTTC CAGAACCTGC AAGTAATCCG GGGACGAATT 
CTGCACAATG GCGCCTACTC GCTGACCCTG CAAGGGCTGG GCATCAGCTG GCTGGGGCTG 
CGCTCACTGA GGGAACTGGG CAGTGGACTG GCCCTCATCC ACCATAACAC CCACCTCTGC 
TTCGTGCACA CGGTGCCCTG GGACCAGCTC TTTCGGAACC CGCACCAAGC TCTGCTCCAC 
ACTGCCAACC GGCCAGAGGA CGAGTGTGTG GGCGAGGGCC TGGCCTGCCA CCAGCTGTGC 
GCCCGAGGGC ACTGCTGGGG TCCAGGGCCC ACCCAGTGTG TCAACTGCAG CCAGTTCCTT 
CGGGGCCAGG AGTGCGTGGA GGAATGCCGA GTACTGCAGG GGCTCCCCAG GGAGTATGTG 
AATGCCAGGC ACTGTTTGCC GTGCCACCCT GAGTGTCAGC CCCAGAATGG CTCAGTGACC 
TGTTTTGGAC CGGAGGCTGA CCAGTGTGTG GCCTGTGCCC ACTATAAGGA CCCTCCCTTC 
TGCGTGGCCC GCTGCCCCAG CGGTGTGAAA CCTGACCTCT CCTACATGCC CATCTGGAAG 
TTTCCAGATG AGGAGGGCGC ATGCCAGCCT TGCCCCATCA ACTGCACCCA CTCCTGTGTG 
GACCTGGATG ACAAGGGCTG CCCCGCCGAG CAGAGAGCCA GCCCTCTGAC GTCCATCATC 
TCTGCGGTGG TTGGCATTCT GCTGGTCGTG GTCTTGGGGG TGGTCTTTGG GATCCTCATC 
AAGCGACGGC AGCAGAAGAT CCGGAAGTAC ACGATGCGGA GACTGCTGCA GGAAACGGAG 
CTGGTGGAGC CGCTGACACC TAGCGGAGCG ATGCCCAACC AGGCGCAGAT GCGGATCCTG 
AAAGAGACGG AGCTGAGGAA GGTGAAGGTG CTTGGATCTG GCGCTTTTGG CACAGTCTAC 
AAGGGCATCT GGATCCCTGA TGGGGAGAAT GTGAAAATTC CAGTGGCCAT CAAAGTGTTG 
AGGGAAAACA CATCCCCCAA AGCCAACAAA GAAATCTTAG ACGAAGCATA CGTGATGGCT 
GGTGTGGGCT CCCCATATGT CTCCCGCCTT CTGGGCATCT GCCTGACATC CACGGTGCAG 
CTGGTGACAC AGCTTATGCC CTATGGCTGC CTCTTAGACC ATGTCCGGGA AAACCGCGGA 
CGCCTGGGCT CCCAGGACCT GCTGAACTGG TGTATGCAGA TTGCCAAGGG GATGAGCTAC 
CTGGAGGATG TGCGGCTCGT ACACAGGGAC TTGGCCGCTC GGAACGTGCT GGTCAAGAGT 
CCCAACCATG TCAAAATTAC AGACTTCGGG CTGGCTCGGC TGCTGGACAT TGACGAGACA 
GAGTACCATG CAGATGGGGG CAAGGTGCCC ATCAAGTGGA TGGCGCTGGA GTCCATTCTC 
CGCCGGCGGT TCACCCACCA GAGTGATGTG TGGAGTTATG GTGTGACTGT GTGGGAGCTG 
ATGACTTTTG GGGCCAAACC TTACGATGGG ATCCCAGCCC GGGAGATCCC TGACCTGCTG 
GAAAAGGGGG AGCGGCTGCC CCAGCCCCCC ATCTGCACCA TTGATGTCTA CATGATCATG 
GTCAAATGTT GGATGATTGA CTCTGAATGT CGGCCAAGAT TCCGGGAGTT GGTGTCTGAA 
TTCTCCCGCA TGGCCAGGGA CCCCCAGCGC TTTGTGGTCA TCCAGAATGA GGACTTGGGC 
CCAGCCAGTC CCTTGGACAG CACCTTCTAC CGCTCACTGC TGGAGGACGA TGACATGGGG 
GACCTGGTGG ATGCTGAGGA GTATCTGGTA CCCCAGCAGG GCTTCTTCTG TCCAGACCCT 
GCCCCGGGCG CTGGGGGCAT GGTCCACCAC AGGCACCGCA GCTCATCTAC CAGGAGTGGC 
GGTGGGGACC TGACACTAGG GCTGGAGCCC TCTGAAGAGG AGGCCCCCAG GTCTCCACTG 
GCACCCTCCG AAGGGGCTGG CTCCGATGTA TTTGATGGTG ACCTGGGAAT GGGGGCAGCC 
AAGGGGCTGC AAAGCCTCCC CACACATGAC CCCAGCCCTC TACAGCGGTA CAGTGAGGAC 
CCCACAGTAC CCCTGCCCTC TGAGACTGAT GGCTACGTTG CCCCCCTGAC CTGCAGCCCC 
CAGCCTGAAT ATGTGAACCA GCCAGATGTT CGGCCCCAGC CCCCTTCGCC CCGAGAGGGC 
CCTCTGCCTG CTGCCCGACC TGCTGGTGCC ACTCTGGAAA GGCCCAAGAC TCTCTCCCCA 
GGGAAGAATG GGGTCGTCAA AGACGTTTTT GCCTTTGGGG GTGCCGTGGA GAACCCCGAG 
TACTTGACAC CCCAGGGAGG AGCTGCCCCT CAGCCCCACC CTCCTCCTGC CTTCAGCCCA 
GCCTTCGACA ACCTCTATTA CTGGGACCAG GACCCACCAG AGCGGGGGGC TCCACCCAGC 
ACCTTCAAAG GGACACCTAC GGCAGAGAAC CCAGAGTACC TGGGTCTGGA CGTGCCAGTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001276865.1
CDS	583..4305
Translation	MPRGSWKPQVCTGTDMKLRLPASPETHLDMLRHLYQGCQVVQGNLELTYLPTNASLSFLQDIQEVQGYVLIAHNQVRQVPLQRLRIVRGTQLFEDNYALAVLDNGDPLNNTTPVTGASPGGLRELQLRSLTEILKGGVLIQRNPQLCYQDTILWKDIFHKNNQLALTLIDTNRSRACHPCSPMCKGSRCWGESSEDCQSLTRTVCAGGCARCKGPLPTDCCHEQCAAGCTGPKHSDCLACLHFNHSGICELHCPALVTYNTDTFESMPNPEGRYTFGASCVTACPYNYLSTDVGSCTLVCPLHNQEVTAEDGTQRCEKCSKPCARVCYGLGMEHLREVRAVTSANIQEFAGCKKIFGSLAFLPESFDGDPASNTAPLQPEQLQVFETLEEITGYLYISAWPDSLPDLSVFQNLQVIRGRILHNGAYSLTLQGLGISWLGLRSLRELGSGLALIHHNTHLCFVHTVPWDQLFRNPHQALLHTANRPEDECVGEGLACHQLCARGHCWGPGPTQCVNCSQFLRGQECVEECRVLQGLPREYVNARHCLPCHPECQPQNGSVTCFGPEADQCVACAHYKDPPFCVARCPSGVKPDLSYMPIWKFPDEEGACQPCPINCTHSCVDLDDKGCPAEQRASPLTSIISAVVGILLVVVLGVVFGILIKRRQQKIRKYTMRRLLQETELVEPLTPSGAMPNQAQMRILKETELRKVKVLGSGAFGTVYKGIWIPDGENVKIPVAIKVLRENTSPKANKEILDEAYVMAGVGSPYVSRLLGICLTSTVQLVTQLMPYGCLLDHVRENRGRLGSQDLLNWCMQIAKGMSYLEDVRLVHRDLAARNVLVKSPNHVKITDFGLARLLDIDETEYHADGGKVPIKWMALESILRRRFTHQSDVWSYGVTVWELMTFGAKPYDGIPAREIPDLLEKGERLPQPPICTIDVYMIMVKCWMIDSECRPRFRELVSEFSRMARDPQRFVVIQNEDLGPASPLDSTFYRSLLEDDDMGDLVDAEEYLVPQQGFFCPDPAPGAGGMVHHRHRSSSTRSGGGDLTLGLEPSEEEAPRSPLAPSEGAGSDVFDGDLGMGAAKGLQSLPTHDPSPLQRYSEDPTVPLPSETDGYVAPLTCSPQPEYVNQPDVRPQPPSPREGPLPAARPAGATLERPKTLSPGKNGVVKDVFAFGGAVENPEYLTPQGGAAPQPHPPPAFSPAFDNLYYWDQDPPERGAPPSTFKGTPTAENPEYLGLDVPV

Target ORF information:

RefSeq Version	NM_001289936.1
Organism	Homo sapiens(human)
Definition	Homo sapiens erb-b2 receptor tyrosine kinase 2 (ERBB2), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001289936.1

ORF Insert Sequence:

ATGCCCCGGG GGTCCTGGAA GCCACAAGTG TGCACCGGCA CAGACATGAA GCTGCGGCTC 
CCTGCCAGTC CCGAGACCCA CCTGGACATG CTCCGCCACC TCTACCAGGG CTGCCAGGTG 
GTGCAGGGAA ACCTGGAACT CACCTACCTG CCCACCAATG CCAGCCTGTC CTTCCTGCAG 
GATATCCAGG AGGTGCAGGG CTACGTGCTC ATCGCTCACA ACCAAGTGAG GCAGGTCCCA 
CTGCAGAGGC TGCGGATTGT GCGAGGCACC CAGCTCTTTG AGGACAACTA TGCCCTGGCC 
GTGCTAGACA ATGGAGACCC GCTGAACAAT ACCACCCCTG TCACAGGGGC CTCCCCAGGA 
GGCCTGCGGG AGCTGCAGCT TCGAAGCCTC ACAGAGATCT TGAAAGGAGG GGTCTTGATC 
CAGCGGAACC CCCAGCTCTG CTACCAGGAC ACGATTTTGT GGAAGGACAT CTTCCACAAG 
AACAACCAGC TGGCTCTCAC ACTGATAGAC ACCAACCGCT CTCGGGCCTG CCACCCCTGT 
TCTCCGATGT GTAAGGGCTC CCGCTGCTGG GGAGAGAGTT CTGAGGATTG TCAGAGCCTG 
ACGCGCACTG TCTGTGCCGG TGGCTGTGCC CGCTGCAAGG GGCCACTGCC CACTGACTGC 
TGCCATGAGC AGTGTGCTGC CGGCTGCACG GGCCCCAAGC ACTCTGACTG CCTGGCCTGC 
CTCCACTTCA ACCACAGTGG CATCTGTGAG CTGCACTGCC CAGCCCTGGT CACCTACAAC 
ACAGACACGT TTGAGTCCAT GCCCAATCCC GAGGGCCGGT ATACATTCGG CGCCAGCTGT 
GTGACTGCCT GTCCCTACAA CTACCTTTCT ACGGACGTGG GATCCTGCAC CCTCGTCTGC 
CCCCTGCACA ACCAAGAGGT GACAGCAGAG GATGGAACAC AGCGGTGTGA GAAGTGCAGC 
AAGCCCTGTG CCCGAGTGTG CTATGGTCTG GGCATGGAGC ACTTGCGAGA GGTGAGGGCA 
GTTACCAGTG CCAATATCCA GGAGTTTGCT GGCTGCAAGA AGATCTTTGG GAGCCTGGCA 
TTTCTGCCGG AGAGCTTTGA TGGGGACCCA GCCTCCAACA CTGCCCCGCT CCAGCCAGAG 
CAGCTCCAAG TGTTTGAGAC TCTGGAAGAG ATCACAGGTT ACCTATACAT CTCAGCATGG 
CCGGACAGCC TGCCTGACCT CAGCGTCTTC CAGAACCTGC AAGTAATCCG GGGACGAATT 
CTGCACAATG GCGCCTACTC GCTGACCCTG CAAGGGCTGG GCATCAGCTG GCTGGGGCTG 
CGCTCACTGA GGGAACTGGG CAGTGGACTG GCCCTCATCC ACCATAACAC CCACCTCTGC 
TTCGTGCACA CGGTGCCCTG GGACCAGCTC TTTCGGAACC CGCACCAAGC TCTGCTCCAC 
ACTGCCAACC GGCCAGAGGA CGAGTGTGTG GGCGAGGGCC TGGCCTGCCA CCAGCTGTGC 
GCCCGAGGGC ACTGCTGGGG TCCAGGGCCC ACCCAGTGTG TCAACTGCAG CCAGTTCCTT 
CGGGGCCAGG AGTGCGTGGA GGAATGCCGA GTACTGCAGG GGCTCCCCAG GGAGTATGTG 
AATGCCAGGC ACTGTTTGCC GTGCCACCCT GAGTGTCAGC CCCAGAATGG CTCAGTGACC 
TGTTTTGGAC CGGAGGCTGA CCAGTGTGTG GCCTGTGCCC ACTATAAGGA CCCTCCCTTC 
TGCGTGGCCC GCTGCCCCAG CGGTGTGAAA CCTGACCTCT CCTACATGCC CATCTGGAAG 
TTTCCAGATG AGGAGGGCGC ATGCCAGCCT TGCCCCATCA ACTGCACCCA CTCCTGTGTG 
GACCTGGATG ACAAGGGCTG CCCCGCCGAG CAGAGAGCCA GCCCTCTGAC GTCCATCATC 
TCTGCGGTGG TTGGCATTCT GCTGGTCGTG GTCTTGGGGG TGGTCTTTGG GATCCTCATC 
AAGCGACGGC AGCAGAAGAT CCGGAAGTAC ACGATGCGGA GACTGCTGCA GGAAACGGAG 
CTGGTGGAGC CGCTGACACC TAGCGGAGCG ATGCCCAACC AGGCGCAGAT GCGGATCCTG 
AAAGAGACGG AGCTGAGGAA GGTGAAGGTG CTTGGATCTG GCGCTTTTGG CACAGTCTAC 
AAGGGCATCT GGATCCCTGA TGGGGAGAAT GTGAAAATTC CAGTGGCCAT CAAAGTGTTG 
AGGGAAAACA CATCCCCCAA AGCCAACAAA GAAATCTTAG ACGAAGCATA CGTGATGGCT 
GGTGTGGGCT CCCCATATGT CTCCCGCCTT CTGGGCATCT GCCTGACATC CACGGTGCAG 
CTGGTGACAC AGCTTATGCC CTATGGCTGC CTCTTAGACC ATGTCCGGGA AAACCGCGGA 
CGCCTGGGCT CCCAGGACCT GCTGAACTGG TGTATGCAGA TTGCCAAGGG GATGAGCTAC 
CTGGAGGATG TGCGGCTCGT ACACAGGGAC TTGGCCGCTC GGAACGTGCT GGTCAAGAGT 
CCCAACCATG TCAAAATTAC AGACTTCGGG CTGGCTCGGC TGCTGGACAT TGACGAGACA 
GAGTACCATG CAGATGGGGG CAAGGTGCCC ATCAAGTGGA TGGCGCTGGA GTCCATTCTC 
CGCCGGCGGT TCACCCACCA GAGTGATGTG TGGAGTTATG GTGTGACTGT GTGGGAGCTG 
ATGACTTTTG GGGCCAAACC TTACGATGGG ATCCCAGCCC GGGAGATCCC TGACCTGCTG 
GAAAAGGGGG AGCGGCTGCC CCAGCCCCCC ATCTGCACCA TTGATGTCTA CATGATCATG 
GTCAAATGTT GGATGATTGA CTCTGAATGT CGGCCAAGAT TCCGGGAGTT GGTGTCTGAA 
TTCTCCCGCA TGGCCAGGGA CCCCCAGCGC TTTGTGGTCA TCCAGAATGA GGACTTGGGC 
CCAGCCAGTC CCTTGGACAG CACCTTCTAC CGCTCACTGC TGGAGGACGA TGACATGGGG 
GACCTGGTGG ATGCTGAGGA GTATCTGGTA CCCCAGCAGG GCTTCTTCTG TCCAGACCCT 
GCCCCGGGCG CTGGGGGCAT GGTCCACCAC AGGCACCGCA GCTCATCTAC CAGGAGTGGC 
GGTGGGGACC TGACACTAGG GCTGGAGCCC TCTGAAGAGG AGGCCCCCAG GTCTCCACTG 
GCACCCTCCG AAGGGGCTGG CTCCGATGTA TTTGATGGTG ACCTGGGAAT GGGGGCAGCC 
AAGGGGCTGC AAAGCCTCCC CACACATGAC CCCAGCCCTC TACAGCGGTA CAGTGAGGAC 
CCCACAGTAC CCCTGCCCTC TGAGACTGAT GGCTACGTTG CCCCCCTGAC CTGCAGCCCC 
CAGCCTGAAT ATGTGAACCA GCCAGATGTT CGGCCCCAGC CCCCTTCGCC CCGAGAGGGC 
CCTCTGCCTG CTGCCCGACC TGCTGGTGCC ACTCTGGAAA GGCCCAAGAC TCTCTCCCCA 
GGGAAGAATG GGGTCGTCAA AGACGTTTTT GCCTTTGGGG GTGCCGTGGA GAACCCCGAG 
TACTTGACAC CCCAGGGAGG AGCTGCCCCT CAGCCCCACC CTCCTCCTGC CTTCAGCCCA 
GCCTTCGACA ACCTCTATTA CTGGGACCAG GACCCACCAG AGCGGGGGGC TCCACCCAGC 
ACCTTCAAAG GGACACCTAC GGCAGAGAAC CCAGAGTACC TGGGTCTGGA CGTGCCAGTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ERBB2 ( NM_001005862.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001005862.1 Homo sapiens erb-b2 receptor tyrosine kinase 2 (ERBB2), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu27580
Clone ID Related Accession (Same CDS sequence)	NM_001005862.2
Accession Version	NM_001005862.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3678bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-03
Organism	Homo sapiens(human)
Product	receptor tyrosine-protein kinase erbB-2 isoform b
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK295195.1, BC110392.1, BC080193.1 and BM678576.1. This sequence is a reference standard in the RefSeqGene project. On Feb 11, 2014 this sequence version replaced NM_001005862.1. Transcript Variant: This variant (2) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at a downstream start codon, compared to variant 1. The resulting isoform (b) has a shorter N-terminus, compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.79901.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAAGCTGC GGCTCCCTGC CAGTCCCGAG ACCCACCTGG ACATGCTCCG CCACCTCTAC 
CAGGGCTGCC AGGTGGTGCA GGGAAACCTG GAACTCACCT ACCTGCCCAC CAATGCCAGC 
CTGTCCTTCC TGCAGGATAT CCAGGAGGTG CAGGGCTACG TGCTCATCGC TCACAACCAA 
GTGAGGCAGG TCCCACTGCA GAGGCTGCGG ATTGTGCGAG GCACCCAGCT CTTTGAGGAC 
AACTATGCCC TGGCCGTGCT AGACAATGGA GACCCGCTGA ACAATACCAC CCCTGTCACA 
GGGGCCTCCC CAGGAGGCCT GCGGGAGCTG CAGCTTCGAA GCCTCACAGA GATCTTGAAA 
GGAGGGGTCT TGATCCAGCG GAACCCCCAG CTCTGCTACC AGGACACGAT TTTGTGGAAG 
GACATCTTCC ACAAGAACAA CCAGCTGGCT CTCACACTGA TAGACACCAA CCGCTCTCGG 
GCCTGCCACC CCTGTTCTCC GATGTGTAAG GGCTCCCGCT GCTGGGGAGA GAGTTCTGAG 
GATTGTCAGA GCCTGACGCG CACTGTCTGT GCCGGTGGCT GTGCCCGCTG CAAGGGGCCA 
CTGCCCACTG ACTGCTGCCA TGAGCAGTGT GCTGCCGGCT GCACGGGCCC CAAGCACTCT 
GACTGCCTGG CCTGCCTCCA CTTCAACCAC AGTGGCATCT GTGAGCTGCA CTGCCCAGCC 
CTGGTCACCT ACAACACAGA CACGTTTGAG TCCATGCCCA ATCCCGAGGG CCGGTATACA 
TTCGGCGCCA GCTGTGTGAC TGCCTGTCCC TACAACTACC TTTCTACGGA CGTGGGATCC 
TGCACCCTCG TCTGCCCCCT GCACAACCAA GAGGTGACAG CAGAGGATGG AACACAGCGG 
TGTGAGAAGT GCAGCAAGCC CTGTGCCCGA GTGTGCTATG GTCTGGGCAT GGAGCACTTG 
CGAGAGGTGA GGGCAGTTAC CAGTGCCAAT ATCCAGGAGT TTGCTGGCTG CAAGAAGATC 
TTTGGGAGCC TGGCATTTCT GCCGGAGAGC TTTGATGGGG ACCCAGCCTC CAACACTGCC 
CCGCTCCAGC CAGAGCAGCT CCAAGTGTTT GAGACTCTGG AAGAGATCAC AGGTTACCTA 
TACATCTCAG CATGGCCGGA CAGCCTGCCT GACCTCAGCG TCTTCCAGAA CCTGCAAGTA 
ATCCGGGGAC GAATTCTGCA CAATGGCGCC TACTCGCTGA CCCTGCAAGG GCTGGGCATC 
AGCTGGCTGG GGCTGCGCTC ACTGAGGGAA CTGGGCAGTG GACTGGCCCT CATCCACCAT 
AACACCCACC TCTGCTTCGT GCACACGGTG CCCTGGGACC AGCTCTTTCG GAACCCGCAC 
CAAGCTCTGC TCCACACTGC CAACCGGCCA GAGGACGAGT GTGTGGGCGA GGGCCTGGCC 
TGCCACCAGC TGTGCGCCCG AGGGCACTGC TGGGGTCCAG GGCCCACCCA GTGTGTCAAC 
TGCAGCCAGT TCCTTCGGGG CCAGGAGTGC GTGGAGGAAT GCCGAGTACT GCAGGGGCTC 
CCCAGGGAGT ATGTGAATGC CAGGCACTGT TTGCCGTGCC ACCCTGAGTG TCAGCCCCAG 
AATGGCTCAG TGACCTGTTT TGGACCGGAG GCTGACCAGT GTGTGGCCTG TGCCCACTAT 
AAGGACCCTC CCTTCTGCGT GGCCCGCTGC CCCAGCGGTG TGAAACCTGA CCTCTCCTAC 
ATGCCCATCT GGAAGTTTCC AGATGAGGAG GGCGCATGCC AGCCTTGCCC CATCAACTGC 
ACCCACTCCT GTGTGGACCT GGATGACAAG GGCTGCCCCG CCGAGCAGAG AGCCAGCCCT 
CTGACGTCCA TCATCTCTGC GGTGGTTGGC ATTCTGCTGG TCGTGGTCTT GGGGGTGGTC 
TTTGGGATCC TCATCAAGCG ACGGCAGCAG AAGATCCGGA AGTACACGAT GCGGAGACTG 
CTGCAGGAAA CGGAGCTGGT GGAGCCGCTG ACACCTAGCG GAGCGATGCC CAACCAGGCG 
CAGATGCGGA TCCTGAAAGA GACGGAGCTG AGGAAGGTGA AGGTGCTTGG ATCTGGCGCT 
TTTGGCACAG TCTACAAGGG CATCTGGATC CCTGATGGGG AGAATGTGAA AATTCCAGTG 
GCCATCAAAG TGTTGAGGGA AAACACATCC CCCAAAGCCA ACAAAGAAAT CTTAGACGAA 
GCATACGTGA TGGCTGGTGT GGGCTCCCCA TATGTCTCCC GCCTTCTGGG CATCTGCCTG 
ACATCCACGG TGCAGCTGGT GACACAGCTT ATGCCCTATG GCTGCCTCTT AGACCATGTC 
CGGGAAAACC GCGGACGCCT GGGCTCCCAG GACCTGCTGA ACTGGTGTAT GCAGATTGCC 
AAGGGGATGA GCTACCTGGA GGATGTGCGG CTCGTACACA GGGACTTGGC CGCTCGGAAC 
GTGCTGGTCA AGAGTCCCAA CCATGTCAAA ATTACAGACT TCGGGCTGGC TCGGCTGCTG 
GACATTGACG AGACAGAGTA CCATGCAGAT GGGGGCAAGG TGCCCATCAA GTGGATGGCG 
CTGGAGTCCA TTCTCCGCCG GCGGTTCACC CACCAGAGTG ATGTGTGGAG TTATGGTGTG 
ACTGTGTGGG AGCTGATGAC TTTTGGGGCC AAACCTTACG ATGGGATCCC AGCCCGGGAG 
ATCCCTGACC TGCTGGAAAA GGGGGAGCGG CTGCCCCAGC CCCCCATCTG CACCATTGAT 
GTCTACATGA TCATGGTCAA ATGTTGGATG ATTGACTCTG AATGTCGGCC AAGATTCCGG 
GAGTTGGTGT CTGAATTCTC CCGCATGGCC AGGGACCCCC AGCGCTTTGT GGTCATCCAG 
AATGAGGACT TGGGCCCAGC CAGTCCCTTG GACAGCACCT TCTACCGCTC ACTGCTGGAG 
GACGATGACA TGGGGGACCT GGTGGATGCT GAGGAGTATC TGGTACCCCA GCAGGGCTTC 
TTCTGTCCAG ACCCTGCCCC GGGCGCTGGG GGCATGGTCC ACCACAGGCA CCGCAGCTCA 
TCTACCAGGA GTGGCGGTGG GGACCTGACA CTAGGGCTGG AGCCCTCTGA AGAGGAGGCC 
CCCAGGTCTC CACTGGCACC CTCCGAAGGG GCTGGCTCCG ATGTATTTGA TGGTGACCTG 
GGAATGGGGG CAGCCAAGGG GCTGCAAAGC CTCCCCACAC ATGACCCCAG CCCTCTACAG 
CGGTACAGTG AGGACCCCAC AGTACCCCTG CCCTCTGAGA CTGATGGCTA CGTTGCCCCC 
CTGACCTGCA GCCCCCAGCC TGAATATGTG AACCAGCCAG ATGTTCGGCC CCAGCCCCCT 
TCGCCCCGAG AGGGCCCTCT GCCTGCTGCC CGACCTGCTG GTGCCACTCT GGAAAGGCCC 
AAGACTCTCT CCCCAGGGAA GAATGGGGTC GTCAAAGACG TTTTTGCCTT TGGGGGTGCC 
GTGGAGAACC CCGAGTACTT GACACCCCAG GGAGGAGCTG CCCCTCAGCC CCACCCTCCT 
CCTGCCTTCA GCCCAGCCTT CGACAACCTC TATTACTGGG ACCAGGACCC ACCAGAGCGG 
GGGGCTCCAC CCAGCACCTT CAAAGGGACA CCTACGGCAG AGAACCCAGA GTACCTGGGT 
CTGGACGTGC CAGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001005862.1
CDS	577..4254
Translation	MKLRLPASPETHLDMLRHLYQGCQVVQGNLELTYLPTNASLSFLQDIQEVQGYVLIAHNQVRQVPLQRLRIVRGTQLFEDNYALAVLDNGDPLNNTTPVTGASPGGLRELQLRSLTEILKGGVLIQRNPQLCYQDTILWKDIFHKNNQLALTLIDTNRSRACHPCSPMCKGSRCWGESSEDCQSLTRTVCAGGCARCKGPLPTDCCHEQCAAGCTGPKHSDCLACLHFNHSGICELHCPALVTYNTDTFESMPNPEGRYTFGASCVTACPYNYLSTDVGSCTLVCPLHNQEVTAEDGTQRCEKCSKPCARVCYGLGMEHLREVRAVTSANIQEFAGCKKIFGSLAFLPESFDGDPASNTAPLQPEQLQVFETLEEITGYLYISAWPDSLPDLSVFQNLQVIRGRILHNGAYSLTLQGLGISWLGLRSLRELGSGLALIHHNTHLCFVHTVPWDQLFRNPHQALLHTANRPEDECVGEGLACHQLCARGHCWGPGPTQCVNCSQFLRGQECVEECRVLQGLPREYVNARHCLPCHPECQPQNGSVTCFGPEADQCVACAHYKDPPFCVARCPSGVKPDLSYMPIWKFPDEEGACQPCPINCTHSCVDLDDKGCPAEQRASPLTSIISAVVGILLVVVLGVVFGILIKRRQQKIRKYTMRRLLQETELVEPLTPSGAMPNQAQMRILKETELRKVKVLGSGAFGTVYKGIWIPDGENVKIPVAIKVLRENTSPKANKEILDEAYVMAGVGSPYVSRLLGICLTSTVQLVTQLMPYGCLLDHVRENRGRLGSQDLLNWCMQIAKGMSYLEDVRLVHRDLAARNVLVKSPNHVKITDFGLARLLDIDETEYHADGGKVPIKWMALESILRRRFTHQSDVWSYGVTVWELMTFGAKPYDGIPAREIPDLLEKGERLPQPPICTIDVYMIMVKCWMIDSECRPRFRELVSEFSRMARDPQRFVVIQNEDLGPASPLDSTFYRSLLEDDDMGDLVDAEEYLVPQQGFFCPDPAPGAGGMVHHRHRSSSTRSGGGDLTLGLEPSEEEAPRSPLAPSEGAGSDVFDGDLGMGAAKGLQSLPTHDPSPLQRYSEDPTVPLPSETDGYVAPLTCSPQPEYVNQPDVRPQPPSPREGPLPAARPAGATLERPKTLSPGKNGVVKDVFAFGGAVENPEYLTPQGGAAPQPHPPPAFSPAFDNLYYWDQDPPERGAPPSTFKGTPTAENPEYLGLDVPV

Target ORF information:

RefSeq Version	NM_001005862.2
Organism	Homo sapiens(human)
Definition	Homo sapiens erb-b2 receptor tyrosine kinase 2 (ERBB2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001005862.2

ORF Insert Sequence:

ATGAAGCTGC GGCTCCCTGC CAGTCCCGAG ACCCACCTGG ACATGCTCCG CCACCTCTAC 
CAGGGCTGCC AGGTGGTGCA GGGAAACCTG GAACTCACCT ACCTGCCCAC CAATGCCAGC 
CTGTCCTTCC TGCAGGATAT CCAGGAGGTG CAGGGCTACG TGCTCATCGC TCACAACCAA 
GTGAGGCAGG TCCCACTGCA GAGGCTGCGG ATTGTGCGAG GCACCCAGCT CTTTGAGGAC 
AACTATGCCC TGGCCGTGCT AGACAATGGA GACCCGCTGA ACAATACCAC CCCTGTCACA 
GGGGCCTCCC CAGGAGGCCT GCGGGAGCTG CAGCTTCGAA GCCTCACAGA GATCTTGAAA 
GGAGGGGTCT TGATCCAGCG GAACCCCCAG CTCTGCTACC AGGACACGAT TTTGTGGAAG 
GACATCTTCC ACAAGAACAA CCAGCTGGCT CTCACACTGA TAGACACCAA CCGCTCTCGG 
GCCTGCCACC CCTGTTCTCC GATGTGTAAG GGCTCCCGCT GCTGGGGAGA GAGTTCTGAG 
GATTGTCAGA GCCTGACGCG CACTGTCTGT GCCGGTGGCT GTGCCCGCTG CAAGGGGCCA 
CTGCCCACTG ACTGCTGCCA TGAGCAGTGT GCTGCCGGCT GCACGGGCCC CAAGCACTCT 
GACTGCCTGG CCTGCCTCCA CTTCAACCAC AGTGGCATCT GTGAGCTGCA CTGCCCAGCC 
CTGGTCACCT ACAACACAGA CACGTTTGAG TCCATGCCCA ATCCCGAGGG CCGGTATACA 
TTCGGCGCCA GCTGTGTGAC TGCCTGTCCC TACAACTACC TTTCTACGGA CGTGGGATCC 
TGCACCCTCG TCTGCCCCCT GCACAACCAA GAGGTGACAG CAGAGGATGG AACACAGCGG 
TGTGAGAAGT GCAGCAAGCC CTGTGCCCGA GTGTGCTATG GTCTGGGCAT GGAGCACTTG 
CGAGAGGTGA GGGCAGTTAC CAGTGCCAAT ATCCAGGAGT TTGCTGGCTG CAAGAAGATC 
TTTGGGAGCC TGGCATTTCT GCCGGAGAGC TTTGATGGGG ACCCAGCCTC CAACACTGCC 
CCGCTCCAGC CAGAGCAGCT CCAAGTGTTT GAGACTCTGG AAGAGATCAC AGGTTACCTA 
TACATCTCAG CATGGCCGGA CAGCCTGCCT GACCTCAGCG TCTTCCAGAA CCTGCAAGTA 
ATCCGGGGAC GAATTCTGCA CAATGGCGCC TACTCGCTGA CCCTGCAAGG GCTGGGCATC 
AGCTGGCTGG GGCTGCGCTC ACTGAGGGAA CTGGGCAGTG GACTGGCCCT CATCCACCAT 
AACACCCACC TCTGCTTCGT GCACACGGTG CCCTGGGACC AGCTCTTTCG GAACCCGCAC 
CAAGCTCTGC TCCACACTGC CAACCGGCCA GAGGACGAGT GTGTGGGCGA GGGCCTGGCC 
TGCCACCAGC TGTGCGCCCG AGGGCACTGC TGGGGTCCAG GGCCCACCCA GTGTGTCAAC 
TGCAGCCAGT TCCTTCGGGG CCAGGAGTGC GTGGAGGAAT GCCGAGTACT GCAGGGGCTC 
CCCAGGGAGT ATGTGAATGC CAGGCACTGT TTGCCGTGCC ACCCTGAGTG TCAGCCCCAG 
AATGGCTCAG TGACCTGTTT TGGACCGGAG GCTGACCAGT GTGTGGCCTG TGCCCACTAT 
AAGGACCCTC CCTTCTGCGT GGCCCGCTGC CCCAGCGGTG TGAAACCTGA CCTCTCCTAC 
ATGCCCATCT GGAAGTTTCC AGATGAGGAG GGCGCATGCC AGCCTTGCCC CATCAACTGC 
ACCCACTCCT GTGTGGACCT GGATGACAAG GGCTGCCCCG CCGAGCAGAG AGCCAGCCCT 
CTGACGTCCA TCATCTCTGC GGTGGTTGGC ATTCTGCTGG TCGTGGTCTT GGGGGTGGTC 
TTTGGGATCC TCATCAAGCG ACGGCAGCAG AAGATCCGGA AGTACACGAT GCGGAGACTG 
CTGCAGGAAA CGGAGCTGGT GGAGCCGCTG ACACCTAGCG GAGCGATGCC CAACCAGGCG 
CAGATGCGGA TCCTGAAAGA GACGGAGCTG AGGAAGGTGA AGGTGCTTGG ATCTGGCGCT 
TTTGGCACAG TCTACAAGGG CATCTGGATC CCTGATGGGG AGAATGTGAA AATTCCAGTG 
GCCATCAAAG TGTTGAGGGA AAACACATCC CCCAAAGCCA ACAAAGAAAT CTTAGACGAA 
GCATACGTGA TGGCTGGTGT GGGCTCCCCA TATGTCTCCC GCCTTCTGGG CATCTGCCTG 
ACATCCACGG TGCAGCTGGT GACACAGCTT ATGCCCTATG GCTGCCTCTT AGACCATGTC 
CGGGAAAACC GCGGACGCCT GGGCTCCCAG GACCTGCTGA ACTGGTGTAT GCAGATTGCC 
AAGGGGATGA GCTACCTGGA GGATGTGCGG CTCGTACACA GGGACTTGGC CGCTCGGAAC 
GTGCTGGTCA AGAGTCCCAA CCATGTCAAA ATTACAGACT TCGGGCTGGC TCGGCTGCTG 
GACATTGACG AGACAGAGTA CCATGCAGAT GGGGGCAAGG TGCCCATCAA GTGGATGGCG 
CTGGAGTCCA TTCTCCGCCG GCGGTTCACC CACCAGAGTG ATGTGTGGAG TTATGGTGTG 
ACTGTGTGGG AGCTGATGAC TTTTGGGGCC AAACCTTACG ATGGGATCCC AGCCCGGGAG 
ATCCCTGACC TGCTGGAAAA GGGGGAGCGG CTGCCCCAGC CCCCCATCTG CACCATTGAT 
GTCTACATGA TCATGGTCAA ATGTTGGATG ATTGACTCTG AATGTCGGCC AAGATTCCGG 
GAGTTGGTGT CTGAATTCTC CCGCATGGCC AGGGACCCCC AGCGCTTTGT GGTCATCCAG 
AATGAGGACT TGGGCCCAGC CAGTCCCTTG GACAGCACCT TCTACCGCTC ACTGCTGGAG 
GACGATGACA TGGGGGACCT GGTGGATGCT GAGGAGTATC TGGTACCCCA GCAGGGCTTC 
TTCTGTCCAG ACCCTGCCCC GGGCGCTGGG GGCATGGTCC ACCACAGGCA CCGCAGCTCA 
TCTACCAGGA GTGGCGGTGG GGACCTGACA CTAGGGCTGG AGCCCTCTGA AGAGGAGGCC 
CCCAGGTCTC CACTGGCACC CTCCGAAGGG GCTGGCTCCG ATGTATTTGA TGGTGACCTG 
GGAATGGGGG CAGCCAAGGG GCTGCAAAGC CTCCCCACAC ATGACCCCAG CCCTCTACAG 
CGGTACAGTG AGGACCCCAC AGTACCCCTG CCCTCTGAGA CTGATGGCTA CGTTGCCCCC 
CTGACCTGCA GCCCCCAGCC TGAATATGTG AACCAGCCAG ATGTTCGGCC CCAGCCCCCT 
TCGCCCCGAG AGGGCCCTCT GCCTGCTGCC CGACCTGCTG GTGCCACTCT GGAAAGGCCC 
AAGACTCTCT CCCCAGGGAA GAATGGGGTC GTCAAAGACG TTTTTGCCTT TGGGGGTGCC 
GTGGAGAACC CCGAGTACTT GACACCCCAG GGAGGAGCTG CCCCTCAGCC CCACCCTCCT 
CCTGCCTTCA GCCCAGCCTT CGACAACCTC TATTACTGGG ACCAGGACCC ACCAGAGCGG 
GGGGCTCCAC CCAGCACCTT CAAAGGGACA CCTACGGCAG AGAACCCAGA GTACCTGGGT 
CTGGACGTGC CAGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ERBB2 ( NM_004448.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_004439.2 Homo sapiens erb-b2 receptor tyrosine kinase 2 (ERBB2), transcript variant 1, mRNA.

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CloneID	OHu27656
Clone ID Related Accession (Same CDS sequence)	NM_004448.3
Accession Version	NM_004448.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3768bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Homo sapiens(human)
Product	receptor tyrosine-protein kinase erbB-2 isoform a precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC346286.1, X03363.1 and BM678576.1. This sequence is a reference standard in the RefSeqGene project. On Feb 11, 2014 this sequence version replaced NM_004448.2. Transcript Variant: This variant (1) encodes the longest isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: M11730.1, X03363.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression regulatory uORF :: PMID: 10446211 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGAGCTGG CGGCCTTGTG CCGCTGGGGG CTCCTCCTCG CCCTCTTGCC CCCCGGAGCC 
GCGAGCACCC AAGTGTGCAC CGGCACAGAC ATGAAGCTGC GGCTCCCTGC CAGTCCCGAG 
ACCCACCTGG ACATGCTCCG CCACCTCTAC CAGGGCTGCC AGGTGGTGCA GGGAAACCTG 
GAACTCACCT ACCTGCCCAC CAATGCCAGC CTGTCCTTCC TGCAGGATAT CCAGGAGGTG 
CAGGGCTACG TGCTCATCGC TCACAACCAA GTGAGGCAGG TCCCACTGCA GAGGCTGCGG 
ATTGTGCGAG GCACCCAGCT CTTTGAGGAC AACTATGCCC TGGCCGTGCT AGACAATGGA 
GACCCGCTGA ACAATACCAC CCCTGTCACA GGGGCCTCCC CAGGAGGCCT GCGGGAGCTG 
CAGCTTCGAA GCCTCACAGA GATCTTGAAA GGAGGGGTCT TGATCCAGCG GAACCCCCAG 
CTCTGCTACC AGGACACGAT TTTGTGGAAG GACATCTTCC ACAAGAACAA CCAGCTGGCT 
CTCACACTGA TAGACACCAA CCGCTCTCGG GCCTGCCACC CCTGTTCTCC GATGTGTAAG 
GGCTCCCGCT GCTGGGGAGA GAGTTCTGAG GATTGTCAGA GCCTGACGCG CACTGTCTGT 
GCCGGTGGCT GTGCCCGCTG CAAGGGGCCA CTGCCCACTG ACTGCTGCCA TGAGCAGTGT 
GCTGCCGGCT GCACGGGCCC CAAGCACTCT GACTGCCTGG CCTGCCTCCA CTTCAACCAC 
AGTGGCATCT GTGAGCTGCA CTGCCCAGCC CTGGTCACCT ACAACACAGA CACGTTTGAG 
TCCATGCCCA ATCCCGAGGG CCGGTATACA TTCGGCGCCA GCTGTGTGAC TGCCTGTCCC 
TACAACTACC TTTCTACGGA CGTGGGATCC TGCACCCTCG TCTGCCCCCT GCACAACCAA 
GAGGTGACAG CAGAGGATGG AACACAGCGG TGTGAGAAGT GCAGCAAGCC CTGTGCCCGA 
GTGTGCTATG GTCTGGGCAT GGAGCACTTG CGAGAGGTGA GGGCAGTTAC CAGTGCCAAT 
ATCCAGGAGT TTGCTGGCTG CAAGAAGATC TTTGGGAGCC TGGCATTTCT GCCGGAGAGC 
TTTGATGGGG ACCCAGCCTC CAACACTGCC CCGCTCCAGC CAGAGCAGCT CCAAGTGTTT 
GAGACTCTGG AAGAGATCAC AGGTTACCTA TACATCTCAG CATGGCCGGA CAGCCTGCCT 
GACCTCAGCG TCTTCCAGAA CCTGCAAGTA ATCCGGGGAC GAATTCTGCA CAATGGCGCC 
TACTCGCTGA CCCTGCAAGG GCTGGGCATC AGCTGGCTGG GGCTGCGCTC ACTGAGGGAA 
CTGGGCAGTG GACTGGCCCT CATCCACCAT AACACCCACC TCTGCTTCGT GCACACGGTG 
CCCTGGGACC AGCTCTTTCG GAACCCGCAC CAAGCTCTGC TCCACACTGC CAACCGGCCA 
GAGGACGAGT GTGTGGGCGA GGGCCTGGCC TGCCACCAGC TGTGCGCCCG AGGGCACTGC 
TGGGGTCCAG GGCCCACCCA GTGTGTCAAC TGCAGCCAGT TCCTTCGGGG CCAGGAGTGC 
GTGGAGGAAT GCCGAGTACT GCAGGGGCTC CCCAGGGAGT ATGTGAATGC CAGGCACTGT 
TTGCCGTGCC ACCCTGAGTG TCAGCCCCAG AATGGCTCAG TGACCTGTTT TGGACCGGAG 
GCTGACCAGT GTGTGGCCTG TGCCCACTAT AAGGACCCTC CCTTCTGCGT GGCCCGCTGC 
CCCAGCGGTG TGAAACCTGA CCTCTCCTAC ATGCCCATCT GGAAGTTTCC AGATGAGGAG 
GGCGCATGCC AGCCTTGCCC CATCAACTGC ACCCACTCCT GTGTGGACCT GGATGACAAG 
GGCTGCCCCG CCGAGCAGAG AGCCAGCCCT CTGACGTCCA TCATCTCTGC GGTGGTTGGC 
ATTCTGCTGG TCGTGGTCTT GGGGGTGGTC TTTGGGATCC TCATCAAGCG ACGGCAGCAG 
AAGATCCGGA AGTACACGAT GCGGAGACTG CTGCAGGAAA CGGAGCTGGT GGAGCCGCTG 
ACACCTAGCG GAGCGATGCC CAACCAGGCG CAGATGCGGA TCCTGAAAGA GACGGAGCTG 
AGGAAGGTGA AGGTGCTTGG ATCTGGCGCT TTTGGCACAG TCTACAAGGG CATCTGGATC 
CCTGATGGGG AGAATGTGAA AATTCCAGTG GCCATCAAAG TGTTGAGGGA AAACACATCC 
CCCAAAGCCA ACAAAGAAAT CTTAGACGAA GCATACGTGA TGGCTGGTGT GGGCTCCCCA 
TATGTCTCCC GCCTTCTGGG CATCTGCCTG ACATCCACGG TGCAGCTGGT GACACAGCTT 
ATGCCCTATG GCTGCCTCTT AGACCATGTC CGGGAAAACC GCGGACGCCT GGGCTCCCAG 
GACCTGCTGA ACTGGTGTAT GCAGATTGCC AAGGGGATGA GCTACCTGGA GGATGTGCGG 
CTCGTACACA GGGACTTGGC CGCTCGGAAC GTGCTGGTCA AGAGTCCCAA CCATGTCAAA 
ATTACAGACT TCGGGCTGGC TCGGCTGCTG GACATTGACG AGACAGAGTA CCATGCAGAT 
GGGGGCAAGG TGCCCATCAA GTGGATGGCG CTGGAGTCCA TTCTCCGCCG GCGGTTCACC 
CACCAGAGTG ATGTGTGGAG TTATGGTGTG ACTGTGTGGG AGCTGATGAC TTTTGGGGCC 
AAACCTTACG ATGGGATCCC AGCCCGGGAG ATCCCTGACC TGCTGGAAAA GGGGGAGCGG 
CTGCCCCAGC CCCCCATCTG CACCATTGAT GTCTACATGA TCATGGTCAA ATGTTGGATG 
ATTGACTCTG AATGTCGGCC AAGATTCCGG GAGTTGGTGT CTGAATTCTC CCGCATGGCC 
AGGGACCCCC AGCGCTTTGT GGTCATCCAG AATGAGGACT TGGGCCCAGC CAGTCCCTTG 
GACAGCACCT TCTACCGCTC ACTGCTGGAG GACGATGACA TGGGGGACCT GGTGGATGCT 
GAGGAGTATC TGGTACCCCA GCAGGGCTTC TTCTGTCCAG ACCCTGCCCC GGGCGCTGGG 
GGCATGGTCC ACCACAGGCA CCGCAGCTCA TCTACCAGGA GTGGCGGTGG GGACCTGACA 
CTAGGGCTGG AGCCCTCTGA AGAGGAGGCC CCCAGGTCTC CACTGGCACC CTCCGAAGGG 
GCTGGCTCCG ATGTATTTGA TGGTGACCTG GGAATGGGGG CAGCCAAGGG GCTGCAAAGC 
CTCCCCACAC ATGACCCCAG CCCTCTACAG CGGTACAGTG AGGACCCCAC AGTACCCCTG 
CCCTCTGAGA CTGATGGCTA CGTTGCCCCC CTGACCTGCA GCCCCCAGCC TGAATATGTG 
AACCAGCCAG ATGTTCGGCC CCAGCCCCCT TCGCCCCGAG AGGGCCCTCT GCCTGCTGCC 
CGACCTGCTG GTGCCACTCT GGAAAGGCCC AAGACTCTCT CCCCAGGGAA GAATGGGGTC 
GTCAAAGACG TTTTTGCCTT TGGGGGTGCC GTGGAGAACC CCGAGTACTT GACACCCCAG 
GGAGGAGCTG CCCCTCAGCC CCACCCTCCT CCTGCCTTCA GCCCAGCCTT CGACAACCTC 
TATTACTGGG ACCAGGACCC ACCAGAGCGG GGGGCTCCAC CCAGCACCTT CAAAGGGACA 
CCTACGGCAG AGAACCCAGA GTACCTGGGT CTGGACGTGC CAGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004439.2
CDS	262..4029
Translation	MELAALCRWGLLLALLPPGAASTQVCTGTDMKLRLPASPETHLDMLRHLYQGCQVVQGNLELTYLPTNASLSFLQDIQEVQGYVLIAHNQVRQVPLQRLRIVRGTQLFEDNYALAVLDNGDPLNNTTPVTGASPGGLRELQLRSLTEILKGGVLIQRNPQLCYQDTILWKDIFHKNNQLALTLIDTNRSRACHPCSPMCKGSRCWGESSEDCQSLTRTVCAGGCARCKGPLPTDCCHEQCAAGCTGPKHSDCLACLHFNHSGICELHCPALVTYNTDTFESMPNPEGRYTFGASCVTACPYNYLSTDVGSCTLVCPLHNQEVTAEDGTQRCEKCSKPCARVCYGLGMEHLREVRAVTSANIQEFAGCKKIFGSLAFLPESFDGDPASNTAPLQPEQLQVFETLEEITGYLYISAWPDSLPDLSVFQNLQVIRGRILHNGAYSLTLQGLGISWLGLRSLRELGSGLALIHHNTHLCFVHTVPWDQLFRNPHQALLHTANRPEDECVGEGLACHQLCARGHCWGPGPTQCVNCSQFLRGQECVEECRVLQGLPREYVNARHCLPCHPECQPQNGSVTCFGPEADQCVACAHYKDPPFCVARCPSGVKPDLSYMPIWKFPDEEGACQPCPINCTHSCVDLDDKGCPAEQRASPLTSIISAVVGILLVVVLGVVFGILIKRRQQKIRKYTMRRLLQETELVEPLTPSGAMPNQAQMRILKETELRKVKVLGSGAFGTVYKGIWIPDGENVKIPVAIKVLRENTSPKANKEILDEAYVMAGVGSPYVSRLLGICLTSTVQLVTQLMPYGCLLDHVRENRGRLGSQDLLNWCMQIAKGMSYLEDVRLVHRDLAARNVLVKSPNHVKITDFGLARLLDIDETEYHADGGKVPIKWMALESILRRRFTHQSDVWSYGVTVWELMTFGAKPYDGIPAREIPDLLEKGERLPQPPICTIDVYMIMVKCWMIDSECRPRFRELVSEFSRMARDPQRFVVIQNEDLGPASPLDSTFYRSLLEDDDMGDLVDAEEYLVPQQGFFCPDPAPGAGGMVHHRHRSSSTRSGGGDLTLGLEPSEEEAPRSPLAPSEGAGSDVFDGDLGMGAAKGLQSLPTHDPSPLQRYSEDPTVPLPSETDGYVAPLTCSPQPEYVNQPDVRPQPPSPREGPLPAARPAGATLERPKTLSPGKNGVVKDVFAFGGAVENPEYLTPQGGAAPQPHPPPAFSPAFDNLYYWDQDPPERGAPPSTFKGTPTAENPEYLGLDVPV

Target ORF information:

RefSeq Version	NM_004448.3
Organism	Homo sapiens(human)
Definition	Homo sapiens erb-b2 receptor tyrosine kinase 2 (ERBB2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004448.3

ORF Insert Sequence:

ATGGAGCTGG CGGCCTTGTG CCGCTGGGGG CTCCTCCTCG CCCTCTTGCC CCCCGGAGCC 
GCGAGCACCC AAGTGTGCAC CGGCACAGAC ATGAAGCTGC GGCTCCCTGC CAGTCCCGAG 
ACCCACCTGG ACATGCTCCG CCACCTCTAC CAGGGCTGCC AGGTGGTGCA GGGAAACCTG 
GAACTCACCT ACCTGCCCAC CAATGCCAGC CTGTCCTTCC TGCAGGATAT CCAGGAGGTG 
CAGGGCTACG TGCTCATCGC TCACAACCAA GTGAGGCAGG TCCCACTGCA GAGGCTGCGG 
ATTGTGCGAG GCACCCAGCT CTTTGAGGAC AACTATGCCC TGGCCGTGCT AGACAATGGA 
GACCCGCTGA ACAATACCAC CCCTGTCACA GGGGCCTCCC CAGGAGGCCT GCGGGAGCTG 
CAGCTTCGAA GCCTCACAGA GATCTTGAAA GGAGGGGTCT TGATCCAGCG GAACCCCCAG 
CTCTGCTACC AGGACACGAT TTTGTGGAAG GACATCTTCC ACAAGAACAA CCAGCTGGCT 
CTCACACTGA TAGACACCAA CCGCTCTCGG GCCTGCCACC CCTGTTCTCC GATGTGTAAG 
GGCTCCCGCT GCTGGGGAGA GAGTTCTGAG GATTGTCAGA GCCTGACGCG CACTGTCTGT 
GCCGGTGGCT GTGCCCGCTG CAAGGGGCCA CTGCCCACTG ACTGCTGCCA TGAGCAGTGT 
GCTGCCGGCT GCACGGGCCC CAAGCACTCT GACTGCCTGG CCTGCCTCCA CTTCAACCAC 
AGTGGCATCT GTGAGCTGCA CTGCCCAGCC CTGGTCACCT ACAACACAGA CACGTTTGAG 
TCCATGCCCA ATCCCGAGGG CCGGTATACA TTCGGCGCCA GCTGTGTGAC TGCCTGTCCC 
TACAACTACC TTTCTACGGA CGTGGGATCC TGCACCCTCG TCTGCCCCCT GCACAACCAA 
GAGGTGACAG CAGAGGATGG AACACAGCGG TGTGAGAAGT GCAGCAAGCC CTGTGCCCGA 
GTGTGCTATG GTCTGGGCAT GGAGCACTTG CGAGAGGTGA GGGCAGTTAC CAGTGCCAAT 
ATCCAGGAGT TTGCTGGCTG CAAGAAGATC TTTGGGAGCC TGGCATTTCT GCCGGAGAGC 
TTTGATGGGG ACCCAGCCTC CAACACTGCC CCGCTCCAGC CAGAGCAGCT CCAAGTGTTT 
GAGACTCTGG AAGAGATCAC AGGTTACCTA TACATCTCAG CATGGCCGGA CAGCCTGCCT 
GACCTCAGCG TCTTCCAGAA CCTGCAAGTA ATCCGGGGAC GAATTCTGCA CAATGGCGCC 
TACTCGCTGA CCCTGCAAGG GCTGGGCATC AGCTGGCTGG GGCTGCGCTC ACTGAGGGAA 
CTGGGCAGTG GACTGGCCCT CATCCACCAT AACACCCACC TCTGCTTCGT GCACACGGTG 
CCCTGGGACC AGCTCTTTCG GAACCCGCAC CAAGCTCTGC TCCACACTGC CAACCGGCCA 
GAGGACGAGT GTGTGGGCGA GGGCCTGGCC TGCCACCAGC TGTGCGCCCG AGGGCACTGC 
TGGGGTCCAG GGCCCACCCA GTGTGTCAAC TGCAGCCAGT TCCTTCGGGG CCAGGAGTGC 
GTGGAGGAAT GCCGAGTACT GCAGGGGCTC CCCAGGGAGT ATGTGAATGC CAGGCACTGT 
TTGCCGTGCC ACCCTGAGTG TCAGCCCCAG AATGGCTCAG TGACCTGTTT TGGACCGGAG 
GCTGACCAGT GTGTGGCCTG TGCCCACTAT AAGGACCCTC CCTTCTGCGT GGCCCGCTGC 
CCCAGCGGTG TGAAACCTGA CCTCTCCTAC ATGCCCATCT GGAAGTTTCC AGATGAGGAG 
GGCGCATGCC AGCCTTGCCC CATCAACTGC ACCCACTCCT GTGTGGACCT GGATGACAAG 
GGCTGCCCCG CCGAGCAGAG AGCCAGCCCT CTGACGTCCA TCATCTCTGC GGTGGTTGGC 
ATTCTGCTGG TCGTGGTCTT GGGGGTGGTC TTTGGGATCC TCATCAAGCG ACGGCAGCAG 
AAGATCCGGA AGTACACGAT GCGGAGACTG CTGCAGGAAA CGGAGCTGGT GGAGCCGCTG 
ACACCTAGCG GAGCGATGCC CAACCAGGCG CAGATGCGGA TCCTGAAAGA GACGGAGCTG 
AGGAAGGTGA AGGTGCTTGG ATCTGGCGCT TTTGGCACAG TCTACAAGGG CATCTGGATC 
CCTGATGGGG AGAATGTGAA AATTCCAGTG GCCATCAAAG TGTTGAGGGA AAACACATCC 
CCCAAAGCCA ACAAAGAAAT CTTAGACGAA GCATACGTGA TGGCTGGTGT GGGCTCCCCA 
TATGTCTCCC GCCTTCTGGG CATCTGCCTG ACATCCACGG TGCAGCTGGT GACACAGCTT 
ATGCCCTATG GCTGCCTCTT AGACCATGTC CGGGAAAACC GCGGACGCCT GGGCTCCCAG 
GACCTGCTGA ACTGGTGTAT GCAGATTGCC AAGGGGATGA GCTACCTGGA GGATGTGCGG 
CTCGTACACA GGGACTTGGC CGCTCGGAAC GTGCTGGTCA AGAGTCCCAA CCATGTCAAA 
ATTACAGACT TCGGGCTGGC TCGGCTGCTG GACATTGACG AGACAGAGTA CCATGCAGAT 
GGGGGCAAGG TGCCCATCAA GTGGATGGCG CTGGAGTCCA TTCTCCGCCG GCGGTTCACC 
CACCAGAGTG ATGTGTGGAG TTATGGTGTG ACTGTGTGGG AGCTGATGAC TTTTGGGGCC 
AAACCTTACG ATGGGATCCC AGCCCGGGAG ATCCCTGACC TGCTGGAAAA GGGGGAGCGG 
CTGCCCCAGC CCCCCATCTG CACCATTGAT GTCTACATGA TCATGGTCAA ATGTTGGATG 
ATTGACTCTG AATGTCGGCC AAGATTCCGG GAGTTGGTGT CTGAATTCTC CCGCATGGCC 
AGGGACCCCC AGCGCTTTGT GGTCATCCAG AATGAGGACT TGGGCCCAGC CAGTCCCTTG 
GACAGCACCT TCTACCGCTC ACTGCTGGAG GACGATGACA TGGGGGACCT GGTGGATGCT 
GAGGAGTATC TGGTACCCCA GCAGGGCTTC TTCTGTCCAG ACCCTGCCCC GGGCGCTGGG 
GGCATGGTCC ACCACAGGCA CCGCAGCTCA TCTACCAGGA GTGGCGGTGG GGACCTGACA 
CTAGGGCTGG AGCCCTCTGA AGAGGAGGCC CCCAGGTCTC CACTGGCACC CTCCGAAGGG 
GCTGGCTCCG ATGTATTTGA TGGTGACCTG GGAATGGGGG CAGCCAAGGG GCTGCAAAGC 
CTCCCCACAC ATGACCCCAG CCCTCTACAG CGGTACAGTG AGGACCCCAC AGTACCCCTG 
CCCTCTGAGA CTGATGGCTA CGTTGCCCCC CTGACCTGCA GCCCCCAGCC TGAATATGTG 
AACCAGCCAG ATGTTCGGCC CCAGCCCCCT TCGCCCCGAG AGGGCCCTCT GCCTGCTGCC 
CGACCTGCTG GTGCCACTCT GGAAAGGCCC AAGACTCTCT CCCCAGGGAA GAATGGGGTC 
GTCAAAGACG TTTTTGCCTT TGGGGGTGCC GTGGAGAACC CCGAGTACTT GACACCCCAG 
GGAGGAGCTG CCCCTCAGCC CCACCCTCCT CCTGCCTTCA GCCCAGCCTT CGACAACCTC 
TATTACTGGG ACCAGGACCC ACCAGAGCGG GGGGCTCCAC CCAGCACCTT CAAAGGGACA 
CCTACGGCAG AGAACCCAGA GTACCTGGGT CTGGACGTGC CAGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ERBB2 ( NM_001289938.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001276867.1 Homo sapiens erb-b2 receptor tyrosine kinase 2 (ERBB2), transcript variant 5, mRNA.

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CloneID	OHu27526
Clone ID Related Accession (Same CDS sequence)	NM_001289938.1
Accession Version	NM_001289938.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1812bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Homo sapiens(human)
Product	receptor tyrosine-protein kinase erbB-2 isoform e
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK295195.1 and BC110392.1. This sequence is a reference standard in the RefSeqGene project. Transcript Variant: This variant (5) differs in the 5' and 3' UTRs and has multiple coding region differences, compared to variant 1. The resulting isoform (e) has a shorter N-terminus and a truncated C-terminus, compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC110392.1, SRR1660807.226158.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END##

ATGAAGCTGC GGCTCCCTGC CAGTCCCGAG ACCCACCTGG ACATGCTCCG CCACCTCTAC 
CAGGGCTGCC AGGTGGTGCA GGGAAACCTG GAACTCACCT ACCTGCCCAC CAATGCCAGC 
CTGTCCTTCC TGCAGGATAT CCAGGAGGTG CAGGGCTACG TGCTCATCGC TCACAACCAA 
GTGAGGCAGG TCCCACTGCA GAGGCTGCGG ATTGTGCGAG GCACCCAGCT CTTTGAGGAC 
AACTATGCCC TGGCCGTGCT AGACAATGGA GACCCGCTGA ACAATACCAC CCCTGTCACA 
GGGGCCTCCC CAGGAGGCCT GCGGGAGCTG CAGCTTCGAA GCCTCACAGA GATCTTGAAA 
GGAGGGGTCT TGATCCAGCG GAACCCCCAG CTCTGCTACC AGGACACGAT TTTGTGGAAG 
GACATCTTCC ACAAGAACAA CCAGCTGGCT CTCACACTGA TAGACACCAA CCGCTCTCGG 
GCCTGCCACC CCTGTTCTCC GATGTGTAAG GGCTCCCGCT GCTGGGGAGA GAGTTCTGAG 
GATTGTCAGA GCCTGACGCG CACTGTCTGT GCCGGTGGCT GTGCCCGCTG CAAGGGGCCA 
CTGCCCACTG ACTGCTGCCA TGAGCAGTGT GCTGCCGGCT GCACGGGCCC CAAGCACTCT 
GACTGCCTGG CCTGCCTCCA CTTCAACCAC AGTGGCATCT GTGAGCTGCA CTGCCCAGCC 
CTGGTCACCT ACAACACAGA CACGTTTGAG TCCATGCCCA ATCCCGAGGG CCGGTATACA 
TTCGGCGCCA GCTGTGTGAC TGCCTGTCCC TACAACTACC TTTCTACGGA CGTGGGATCC 
TGCACCCTCG TCTGCCCCCT GCACAACCAA GAGGTGACAG CAGAGGATGG AACACAGCGG 
TGTGAGAAGT GCAGCAAGCC CTGTGCCCGA GTGTGCTATG GTCTGGGCAT GGAGCACTTG 
CGAGAGGTGA GGGCAGTTAC CAGTGCCAAT ATCCAGGAGT TTGCTGGCTG CAAGAAGATC 
TTTGGGAGCC TGGCATTTCT GCCGGAGAGC TTTGATGGGG ACCCAGCCTC CAACACTGCC 
CCGCTCCAGC CAGAGCAGCT CCAAGTGTTT GAGACTCTGG AAGAGATCAC AGGTTACCTA 
TACATCTCAG CATGGCCGGA CAGCCTGCCT GACCTCAGCG TCTTCCAGAA CCTGCAAGTA 
ATCCGGGGAC GAATTCTGCA CAATGGCGCC TACTCGCTGA CCCTGCAAGG GCTGGGCATC 
AGCTGGCTGG GGCTGCGCTC ACTGAGGGAA CTGGGCAGTG GACTGGCCCT CATCCACCAT 
AACACCCACC TCTGCTTCGT GCACACGGTG CCCTGGGACC AGCTCTTTCG GAACCCGCAC 
CAAGCTCTGC TCCACACTGC CAACCGGCCA GAGGACGAGT GTGTGGGCGA GGGCCTGGCC 
TGCCACCAGC TGTGCGCCCG AGGGCACTGC TGGGGTCCAG GGCCCACCCA GTGTGTCAAC 
TGCAGCCAGT TCCTTCGGGG CCAGGAGTGC GTGGAGGAAT GCCGAGTACT GCAGGGGCTC 
CCCAGGGAGT ATGTGAATGC CAGGCACTGT TTGCCGTGCC ACCCTGAGTG TCAGCCCCAG 
AATGGCTCAG TGACCTGTTT TGGACCGGAG GCTGACCAGT GTGTGGCCTG TGCCCACTAT 
AAGGACCCTC CCTTCTGCGT GGCCCGCTGC CCCAGCGGTG TGAAACCTGA CCTCTCCTAC 
ATGCCCATCT GGAAGTTTCC AGATGAGGAG GGCGCATGCC AGCCTTGCCC CATCAACTGC 
ACCCACTCGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001276867.1
CDS	577..2388
Translation	MKLRLPASPETHLDMLRHLYQGCQVVQGNLELTYLPTNASLSFLQDIQEVQGYVLIAHNQVRQVPLQRLRIVRGTQLFEDNYALAVLDNGDPLNNTTPVTGASPGGLRELQLRSLTEILKGGVLIQRNPQLCYQDTILWKDIFHKNNQLALTLIDTNRSRACHPCSPMCKGSRCWGESSEDCQSLTRTVCAGGCARCKGPLPTDCCHEQCAAGCTGPKHSDCLACLHFNHSGICELHCPALVTYNTDTFESMPNPEGRYTFGASCVTACPYNYLSTDVGSCTLVCPLHNQEVTAEDGTQRCEKCSKPCARVCYGLGMEHLREVRAVTSANIQEFAGCKKIFGSLAFLPESFDGDPASNTAPLQPEQLQVFETLEEITGYLYISAWPDSLPDLSVFQNLQVIRGRILHNGAYSLTLQGLGISWLGLRSLRELGSGLALIHHNTHLCFVHTVPWDQLFRNPHQALLHTANRPEDECVGEGLACHQLCARGHCWGPGPTQCVNCSQFLRGQECVEECRVLQGLPREYVNARHCLPCHPECQPQNGSVTCFGPEADQCVACAHYKDPPFCVARCPSGVKPDLSYMPIWKFPDEEGACQPCPINCTHS

Target ORF information:

RefSeq Version	NM_001289938.1
Organism	Homo sapiens(human)
Definition	Homo sapiens erb-b2 receptor tyrosine kinase 2 (ERBB2), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001289938.1

ORF Insert Sequence:

ATGAAGCTGC GGCTCCCTGC CAGTCCCGAG ACCCACCTGG ACATGCTCCG CCACCTCTAC 
CAGGGCTGCC AGGTGGTGCA GGGAAACCTG GAACTCACCT ACCTGCCCAC CAATGCCAGC 
CTGTCCTTCC TGCAGGATAT CCAGGAGGTG CAGGGCTACG TGCTCATCGC TCACAACCAA 
GTGAGGCAGG TCCCACTGCA GAGGCTGCGG ATTGTGCGAG GCACCCAGCT CTTTGAGGAC 
AACTATGCCC TGGCCGTGCT AGACAATGGA GACCCGCTGA ACAATACCAC CCCTGTCACA 
GGGGCCTCCC CAGGAGGCCT GCGGGAGCTG CAGCTTCGAA GCCTCACAGA GATCTTGAAA 
GGAGGGGTCT TGATCCAGCG GAACCCCCAG CTCTGCTACC AGGACACGAT TTTGTGGAAG 
GACATCTTCC ACAAGAACAA CCAGCTGGCT CTCACACTGA TAGACACCAA CCGCTCTCGG 
GCCTGCCACC CCTGTTCTCC GATGTGTAAG GGCTCCCGCT GCTGGGGAGA GAGTTCTGAG 
GATTGTCAGA GCCTGACGCG CACTGTCTGT GCCGGTGGCT GTGCCCGCTG CAAGGGGCCA 
CTGCCCACTG ACTGCTGCCA TGAGCAGTGT GCTGCCGGCT GCACGGGCCC CAAGCACTCT 
GACTGCCTGG CCTGCCTCCA CTTCAACCAC AGTGGCATCT GTGAGCTGCA CTGCCCAGCC 
CTGGTCACCT ACAACACAGA CACGTTTGAG TCCATGCCCA ATCCCGAGGG CCGGTATACA 
TTCGGCGCCA GCTGTGTGAC TGCCTGTCCC TACAACTACC TTTCTACGGA CGTGGGATCC 
TGCACCCTCG TCTGCCCCCT GCACAACCAA GAGGTGACAG CAGAGGATGG AACACAGCGG 
TGTGAGAAGT GCAGCAAGCC CTGTGCCCGA GTGTGCTATG GTCTGGGCAT GGAGCACTTG 
CGAGAGGTGA GGGCAGTTAC CAGTGCCAAT ATCCAGGAGT TTGCTGGCTG CAAGAAGATC 
TTTGGGAGCC TGGCATTTCT GCCGGAGAGC TTTGATGGGG ACCCAGCCTC CAACACTGCC 
CCGCTCCAGC CAGAGCAGCT CCAAGTGTTT GAGACTCTGG AAGAGATCAC AGGTTACCTA 
TACATCTCAG CATGGCCGGA CAGCCTGCCT GACCTCAGCG TCTTCCAGAA CCTGCAAGTA 
ATCCGGGGAC GAATTCTGCA CAATGGCGCC TACTCGCTGA CCCTGCAAGG GCTGGGCATC 
AGCTGGCTGG GGCTGCGCTC ACTGAGGGAA CTGGGCAGTG GACTGGCCCT CATCCACCAT 
AACACCCACC TCTGCTTCGT GCACACGGTG CCCTGGGACC AGCTCTTTCG GAACCCGCAC 
CAAGCTCTGC TCCACACTGC CAACCGGCCA GAGGACGAGT GTGTGGGCGA GGGCCTGGCC 
TGCCACCAGC TGTGCGCCCG AGGGCACTGC TGGGGTCCAG GGCCCACCCA GTGTGTCAAC 
TGCAGCCAGT TCCTTCGGGG CCAGGAGTGC GTGGAGGAAT GCCGAGTACT GCAGGGGCTC 
CCCAGGGAGT ATGTGAATGC CAGGCACTGT TTGCCGTGCC ACCCTGAGTG TCAGCCCCAG 
AATGGCTCAG TGACCTGTTT TGGACCGGAG GCTGACCAGT GTGTGGCCTG TGCCCACTAT 
AAGGACCCTC CCTTCTGCGT GGCCCGCTGC CCCAGCGGTG TGAAACCTGA CCTCTCCTAC 
ATGCCCATCT GGAAGTTTCC AGATGAGGAG GGCGCATGCC AGCCTTGCCC CATCAACTGC 
ACCCACTCGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ERBB2 ( XM_024450641.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024306409.1 Homo sapiens erb-b2 receptor tyrosine kinase 2 (ERBB2), transcript variant X1, mRNA.

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CloneID	OHu104276
Clone ID Related Accession (Same CDS sequence)	XM_024450641.1
Accession Version	XM_024450641.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3906bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	receptor tyrosine-protein kinase erbB-2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCTGG CGGCCTTGTG CCGCTGGGGG CTCCTCCTCG CCCTCTTGCC CCCCGGAGCC 
GCGAGCACCC AAGTGTGCAC CGGCACAGAC ATGAAGCTGC GGCTCCCTGC CAGTCCCGAG 
ACCCACCTGG ACATGCTCCG CCACCTCTAC CAGGGCTGCC AGGTGGTGCA GGGAAACCTG 
GAACTCACCT ACCTGCCCAC CAATGCCAGC CTGTCCTTCC TGCAGGATAT CCAGGAGGTG 
CAGGGCTACG TGCTCATCGC TCACAACCAA GTGAGGCAGG TCCCACTGCA GAGGCTGCGG 
ATTGTGCGAG GCACCCAGCT CTTTGAGGAC AACTATGCCC TGGCCGTGCT AGACAATGGA 
GACCCGCTGA ACAATACCAC CCCTGTCACA GGGGCCTCCC CAGGAGGCCT GCGGGAGCTG 
CAGCTTCGAA GCCTCACAGA GATCTTGAAA GGAGGGGTCT TGATCCAGCG GAACCCCCAG 
CTCTGCTACC AGGACACGAT TTTGTGGAAG GACATCTTCC ACAAGAACAA CCAGCTGGCT 
CTCACACTGA TAGACACCAA CCGCTCTCGG GCCTGCCACC CCTGTTCTCC GATGTGTAAG 
GGCTCCCGCT GCTGGGGAGA GAGTTCTGAG GATTGTCAGA GCCTGACGCG CACTGTCTGT 
GCCGGTGGCT GTGCCCGCTG CAAGGGGCCA CTGCCCACTG ACTGCTGCCA TGAGCAGTGT 
GCTGCCGGCT GCACGGGCCC CAAGCACTCT GACTGCCTGG TATGTGCCTC TGCTTTGTGC 
CCAATGTGCT CTACCCCCCA GGATGCAAGG GGTGGGCACC CTGCCTGGTA CTGCCCTATT 
GCCCCTGGCA CACCAGGGCA AAACAGCACA GTGAAAGCCA GCCACCTGTC CCCCCAGGCC 
TGCCTCCACT TCAACCACAG TGGCATCTGT GAGCTGCACT GCCCAGCCCT GGTCACCTAC 
AACACAGACA CGTTTGAGTC CATGCCCAAT CCCGAGGGCC GGTATACATT CGGCGCCAGC 
TGTGTGACTG CCTGTCCCTA CAACTACCTT TCTACGGACG TGGGATCCTG CACCCTCGTC 
TGCCCCCTGC ACAACCAAGA GGTGACAGCA GAGGATGGAA CACAGCGGTG TGAGAAGTGC 
AGCAAGCCCT GTGCCCGAGT GTGCTATGGT CTGGGCATGG AGCACTTGCG AGAGGTGAGG 
GCAGTTACCA GTGCCAATAT CCAGGAGTTT GCTGGCTGCA AGAAGATCTT TGGGAGCCTG 
GCATTTCTGC CGGAGAGCTT TGATGGGGAC CCAGCCTCCA ACACTGCCCC GCTCCAGCCA 
GAGCAGCTCC AAGTGTTTGA GACTCTGGAA GAGATCACAG GTTACCTATA CATCTCAGCA 
TGGCCGGACA GCCTGCCTGA CCTCAGCGTC TTCCAGAACC TGCAAGTAAT CCGGGGACGA 
ATTCTGCACA ATGGCGCCTA CTCGCTGACC CTGCAAGGGC TGGGCATCAG CTGGCTGGGG 
CTGCGCTCAC TGAGGGAACT GGGCAGTGGA CTGGCCCTCA TCCACCATAA CACCCACCTC 
TGCTTCGTGC ACACGGTGCC CTGGGACCAG CTCTTTCGGA ACCCGCACCA AGCTCTGCTC 
CACACTGCCA ACCGGCCAGA GGACGAGTGT GTGGGCGAGG GCCTGGCCTG CCACCAGCTG 
TGCGCCCGAG GGCACTGCTG GGGTCCAGGG CCCACCCAGT GTGTCAACTG CAGCCAGTTC 
CTTCGGGGCC AGGAGTGCGT GGAGGAATGC CGAGTACTGC AGGGGCTCCC CAGGGAGTAT 
GTGAATGCCA GGCACTGTTT GCCGTGCCAC CCTGAGTGTC AGCCCCAGAA TGGCTCAGTG 
ACCTGTTTTG GACCGGAGGC TGACCAGTGT GTGGCCTGTG CCCACTATAA GGACCCTCCC 
TTCTGCGTGG CCCGCTGCCC CAGCGGTGTG AAACCTGACC TCTCCTACAT GCCCATCTGG 
AAGTTTCCAG ATGAGGAGGG CGCATGCCAG CCTTGCCCCA TCAACTGCAC CCACTCCTGT 
GTGGACCTGG ATGACAAGGG CTGCCCCGCC GAGCAGAGAG CCAGCCCTCT GACGTCCATC 
ATCTCTGCGG TGGTTGGCAT TCTGCTGGTC GTGGTCTTGG GGGTGGTCTT TGGGATCCTC 
ATCAAGCGAC GGCAGCAGAA GATCCGGAAG TACACGATGC GGAGACTGCT GCAGGAAACG 
GAGCTGGTGG AGCCGCTGAC ACCTAGCGGA GCGATGCCCA ACCAGGCGCA GATGCGGATC 
CTGAAAGAGA CGGAGCTGAG GAAGGTGAAG GTGCTTGGAT CTGGCGCTTT TGGCACAGTC 
TACAAGGGCA TCTGGATCCC TGATGGGGAG AATGTGAAAA TTCCAGTGGC CATCAAAGTG 
TTGAGGGAAA ACACATCCCC CAAAGCCAAC AAAGAAATCT TAGACGAAGC ATACGTGATG 
GCTGGTGTGG GCTCCCCATA TGTCTCCCGC CTTCTGGGCA TCTGCCTGAC ATCCACGGTG 
CAGCTGGTGA CACAGCTTAT GCCCTATGGC TGCCTCTTAG ACCATGTCCG GGAAAACCGC 
GGACGCCTGG GCTCCCAGGA CCTGCTGAAC TGGTGTATGC AGATTGCCAA GGGGATGAGC 
TACCTGGAGG ATGTGCGGCT CGTACACAGG GACTTGGCCG CTCGGAACGT GCTGGTCAAG 
AGTCCCAACC ATGTCAAAAT TACAGACTTC GGGCTGGCTC GGCTGCTGGA CATTGACGAG 
ACAGAGTACC ATGCAGATGG GGGCAAGGTG CCCATCAAGT GGATGGCGCT GGAGTCCATT 
CTCCGCCGGC GGTTCACCCA CCAGAGTGAT GTGTGGAGTT ATGGTGTGAC TGTGTGGGAG 
CTGATGACTT TTGGGGCCAA ACCTTACGAT GGGATCCCAG CCCGGGAGAT CCCTGACCTG 
CTGGAAAAGG GGGAGCGGCT GCCCCAGCCC CCCATCTGCA CCATTGATGT CTACATGATC 
ATGGTCAAAT GTTGGATGAT TGACTCTGAA TGTCGGCCAA GATTCCGGGA GTTGGTGTCT 
GAATTCTCCC GCATGGCCAG GGACCCCCAG CGCTTTGTGG TCATCCAGAA TGAGGACTTG 
GGCCCAGCCA GTCCCTTGGA CAGCACCTTC TACCGCTCAC TGCTGGAGGA CGATGACATG 
GGGGACCTGG TGGATGCTGA GGAGTATCTG GTACCCCAGC AGGGCTTCTT CTGTCCAGAC 
CCTGCCCCGG GCGCTGGGGG CATGGTCCAC CACAGGCACC GCAGCTCATC TACCAGGAGT 
GGCGGTGGGG ACCTGACACT AGGGCTGGAG CCCTCTGAAG AGGAGGCCCC CAGGTCTCCA 
CTGGCACCCT CCGAAGGGGC TGGCTCCGAT GTATTTGATG GTGACCTGGG AATGGGGGCA 
GCCAAGGGGC TGCAAAGCCT CCCCACACAT GACCCCAGCC CTCTACAGCG GTACAGTGAG 
GACCCCACAG TACCCCTGCC CTCTGAGACT GATGGCTACG TTGCCCCCCT GACCTGCAGC 
CCCCAGCCTG AATATGTGAA CCAGCCAGAT GTTCGGCCCC AGCCCCCTTC GCCCCGAGAG 
GGCCCTCTGC CTGCTGCCCG ACCTGCTGGT GCCACTCTGG AAAGGCCCAA GACTCTCTCC 
CCAGGGAAGA ATGGGGTCGT CAAAGACGTT TTTGCCTTTG GGGGTGCCGT GGAGAACCCC 
GAGTACTTGA CACCCCAGGG AGGAGCTGCC CCTCAGCCCC ACCCTCCTCC TGCCTTCAGC 
CCAGCCTTCG ACAACCTCTA TTACTGGGAC CAGGACCCAC CAGAGCGGGG GGCTCCACCC 
AGCACCTTCA AAGGGACACC TACGGCAGAG AACCCAGAGT ACCTGGGTCT GGACGTGCCA 
GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024306409.1
CDS	248..4153
Translation	MELAALCRWGLLLALLPPGAASTQVCTGTDMKLRLPASPETHLDMLRHLYQGCQVVQGNLELTYLPTNASLSFLQDIQEVQGYVLIAHNQVRQVPLQRLRIVRGTQLFEDNYALAVLDNGDPLNNTTPVTGASPGGLRELQLRSLTEILKGGVLIQRNPQLCYQDTILWKDIFHKNNQLALTLIDTNRSRACHPCSPMCKGSRCWGESSEDCQSLTRTVCAGGCARCKGPLPTDCCHEQCAAGCTGPKHSDCLVCASALCPMCSTPQDARGGHPAWYCPIAPGTPGQNSTVKASHLSPQACLHFNHSGICELHCPALVTYNTDTFESMPNPEGRYTFGASCVTACPYNYLSTDVGSCTLVCPLHNQEVTAEDGTQRCEKCSKPCARVCYGLGMEHLREVRAVTSANIQEFAGCKKIFGSLAFLPESFDGDPASNTAPLQPEQLQVFETLEEITGYLYISAWPDSLPDLSVFQNLQVIRGRILHNGAYSLTLQGLGISWLGLRSLRELGSGLALIHHNTHLCFVHTVPWDQLFRNPHQALLHTANRPEDECVGEGLACHQLCARGHCWGPGPTQCVNCSQFLRGQECVEECRVLQGLPREYVNARHCLPCHPECQPQNGSVTCFGPEADQCVACAHYKDPPFCVARCPSGVKPDLSYMPIWKFPDEEGACQPCPINCTHSCVDLDDKGCPAEQRASPLTSIISAVVGILLVVVLGVVFGILIKRRQQKIRKYTMRRLLQETELVEPLTPSGAMPNQAQMRILKETELRKVKVLGSGAFGTVYKGIWIPDGENVKIPVAIKVLRENTSPKANKEILDEAYVMAGVGSPYVSRLLGICLTSTVQLVTQLMPYGCLLDHVRENRGRLGSQDLLNWCMQIAKGMSYLEDVRLVHRDLAARNVLVKSPNHVKITDFGLARLLDIDETEYHADGGKVPIKWMALESILRRRFTHQSDVWSYGVTVWELMTFGAKPYDGIPAREIPDLLEKGERLPQPPICTIDVYMIMVKCWMIDSECRPRFRELVSEFSRMARDPQRFVVIQNEDLGPASPLDSTFYRSLLEDDDMGDLVDAEEYLVPQQGFFCPDPAPGAGGMVHHRHRSSSTRSGGGDLTLGLEPSEEEAPRSPLAPSEGAGSDVFDGDLGMGAAKGLQSLPTHDPSPLQRYSEDPTVPLPSETDGYVAPLTCSPQPEYVNQPDVRPQPPSPREGPLPAARPAGATLERPKTLSPGKNGVVKDVFAFGGAVENPEYLTPQGGAAPQPHPPPAFSPAFDNLYYWDQDPPERGAPPSTFKGTPTAENPEYLGLDVPV

Target ORF information:

RefSeq Version	XM_024450641.1
Organism	Homo sapiens(human)
Definition	Homo sapiens erb-b2 receptor tyrosine kinase 2 (ERBB2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024450641.1

ORF Insert Sequence:

ATGGAGCTGG CGGCCTTGTG CCGCTGGGGG CTCCTCCTCG CCCTCTTGCC CCCCGGAGCC 
GCGAGCACCC AAGTGTGCAC CGGCACAGAC ATGAAGCTGC GGCTCCCTGC CAGTCCCGAG 
ACCCACCTGG ACATGCTCCG CCACCTCTAC CAGGGCTGCC AGGTGGTGCA GGGAAACCTG 
GAACTCACCT ACCTGCCCAC CAATGCCAGC CTGTCCTTCC TGCAGGATAT CCAGGAGGTG 
CAGGGCTACG TGCTCATCGC TCACAACCAA GTGAGGCAGG TCCCACTGCA GAGGCTGCGG 
ATTGTGCGAG GCACCCAGCT CTTTGAGGAC AACTATGCCC TGGCCGTGCT AGACAATGGA 
GACCCGCTGA ACAATACCAC CCCTGTCACA GGGGCCTCCC CAGGAGGCCT GCGGGAGCTG 
CAGCTTCGAA GCCTCACAGA GATCTTGAAA GGAGGGGTCT TGATCCAGCG GAACCCCCAG 
CTCTGCTACC AGGACACGAT TTTGTGGAAG GACATCTTCC ACAAGAACAA CCAGCTGGCT 
CTCACACTGA TAGACACCAA CCGCTCTCGG GCCTGCCACC CCTGTTCTCC GATGTGTAAG 
GGCTCCCGCT GCTGGGGAGA GAGTTCTGAG GATTGTCAGA GCCTGACGCG CACTGTCTGT 
GCCGGTGGCT GTGCCCGCTG CAAGGGGCCA CTGCCCACTG ACTGCTGCCA TGAGCAGTGT 
GCTGCCGGCT GCACGGGCCC CAAGCACTCT GACTGCCTGG TATGTGCCTC TGCTTTGTGC 
CCAATGTGCT CTACCCCCCA GGATGCAAGG GGTGGGCACC CTGCCTGGTA CTGCCCTATT 
GCCCCTGGCA CACCAGGGCA AAACAGCACA GTGAAAGCCA GCCACCTGTC CCCCCAGGCC 
TGCCTCCACT TCAACCACAG TGGCATCTGT GAGCTGCACT GCCCAGCCCT GGTCACCTAC 
AACACAGACA CGTTTGAGTC CATGCCCAAT CCCGAGGGCC GGTATACATT CGGCGCCAGC 
TGTGTGACTG CCTGTCCCTA CAACTACCTT TCTACGGACG TGGGATCCTG CACCCTCGTC 
TGCCCCCTGC ACAACCAAGA GGTGACAGCA GAGGATGGAA CACAGCGGTG TGAGAAGTGC 
AGCAAGCCCT GTGCCCGAGT GTGCTATGGT CTGGGCATGG AGCACTTGCG AGAGGTGAGG 
GCAGTTACCA GTGCCAATAT CCAGGAGTTT GCTGGCTGCA AGAAGATCTT TGGGAGCCTG 
GCATTTCTGC CGGAGAGCTT TGATGGGGAC CCAGCCTCCA ACACTGCCCC GCTCCAGCCA 
GAGCAGCTCC AAGTGTTTGA GACTCTGGAA GAGATCACAG GTTACCTATA CATCTCAGCA 
TGGCCGGACA GCCTGCCTGA CCTCAGCGTC TTCCAGAACC TGCAAGTAAT CCGGGGACGA 
ATTCTGCACA ATGGCGCCTA CTCGCTGACC CTGCAAGGGC TGGGCATCAG CTGGCTGGGG 
CTGCGCTCAC TGAGGGAACT GGGCAGTGGA CTGGCCCTCA TCCACCATAA CACCCACCTC 
TGCTTCGTGC ACACGGTGCC CTGGGACCAG CTCTTTCGGA ACCCGCACCA AGCTCTGCTC 
CACACTGCCA ACCGGCCAGA GGACGAGTGT GTGGGCGAGG GCCTGGCCTG CCACCAGCTG 
TGCGCCCGAG GGCACTGCTG GGGTCCAGGG CCCACCCAGT GTGTCAACTG CAGCCAGTTC 
CTTCGGGGCC AGGAGTGCGT GGAGGAATGC CGAGTACTGC AGGGGCTCCC CAGGGAGTAT 
GTGAATGCCA GGCACTGTTT GCCGTGCCAC CCTGAGTGTC AGCCCCAGAA TGGCTCAGTG 
ACCTGTTTTG GACCGGAGGC TGACCAGTGT GTGGCCTGTG CCCACTATAA GGACCCTCCC 
TTCTGCGTGG CCCGCTGCCC CAGCGGTGTG AAACCTGACC TCTCCTACAT GCCCATCTGG 
AAGTTTCCAG ATGAGGAGGG CGCATGCCAG CCTTGCCCCA TCAACTGCAC CCACTCCTGT 
GTGGACCTGG ATGACAAGGG CTGCCCCGCC GAGCAGAGAG CCAGCCCTCT GACGTCCATC 
ATCTCTGCGG TGGTTGGCAT TCTGCTGGTC GTGGTCTTGG GGGTGGTCTT TGGGATCCTC 
ATCAAGCGAC GGCAGCAGAA GATCCGGAAG TACACGATGC GGAGACTGCT GCAGGAAACG 
GAGCTGGTGG AGCCGCTGAC ACCTAGCGGA GCGATGCCCA ACCAGGCGCA GATGCGGATC 
CTGAAAGAGA CGGAGCTGAG GAAGGTGAAG GTGCTTGGAT CTGGCGCTTT TGGCACAGTC 
TACAAGGGCA TCTGGATCCC TGATGGGGAG AATGTGAAAA TTCCAGTGGC CATCAAAGTG 
TTGAGGGAAA ACACATCCCC CAAAGCCAAC AAAGAAATCT TAGACGAAGC ATACGTGATG 
GCTGGTGTGG GCTCCCCATA TGTCTCCCGC CTTCTGGGCA TCTGCCTGAC ATCCACGGTG 
CAGCTGGTGA CACAGCTTAT GCCCTATGGC TGCCTCTTAG ACCATGTCCG GGAAAACCGC 
GGACGCCTGG GCTCCCAGGA CCTGCTGAAC TGGTGTATGC AGATTGCCAA GGGGATGAGC 
TACCTGGAGG ATGTGCGGCT CGTACACAGG GACTTGGCCG CTCGGAACGT GCTGGTCAAG 
AGTCCCAACC ATGTCAAAAT TACAGACTTC GGGCTGGCTC GGCTGCTGGA CATTGACGAG 
ACAGAGTACC ATGCAGATGG GGGCAAGGTG CCCATCAAGT GGATGGCGCT GGAGTCCATT 
CTCCGCCGGC GGTTCACCCA CCAGAGTGAT GTGTGGAGTT ATGGTGTGAC TGTGTGGGAG 
CTGATGACTT TTGGGGCCAA ACCTTACGAT GGGATCCCAG CCCGGGAGAT CCCTGACCTG 
CTGGAAAAGG GGGAGCGGCT GCCCCAGCCC CCCATCTGCA CCATTGATGT CTACATGATC 
ATGGTCAAAT GTTGGATGAT TGACTCTGAA TGTCGGCCAA GATTCCGGGA GTTGGTGTCT 
GAATTCTCCC GCATGGCCAG GGACCCCCAG CGCTTTGTGG TCATCCAGAA TGAGGACTTG 
GGCCCAGCCA GTCCCTTGGA CAGCACCTTC TACCGCTCAC TGCTGGAGGA CGATGACATG 
GGGGACCTGG TGGATGCTGA GGAGTATCTG GTACCCCAGC AGGGCTTCTT CTGTCCAGAC 
CCTGCCCCGG GCGCTGGGGG CATGGTCCAC CACAGGCACC GCAGCTCATC TACCAGGAGT 
GGCGGTGGGG ACCTGACACT AGGGCTGGAG CCCTCTGAAG AGGAGGCCCC CAGGTCTCCA 
CTGGCACCCT CCGAAGGGGC TGGCTCCGAT GTATTTGATG GTGACCTGGG AATGGGGGCA 
GCCAAGGGGC TGCAAAGCCT CCCCACACAT GACCCCAGCC CTCTACAGCG GTACAGTGAG 
GACCCCACAG TACCCCTGCC CTCTGAGACT GATGGCTACG TTGCCCCCCT GACCTGCAGC 
CCCCAGCCTG AATATGTGAA CCAGCCAGAT GTTCGGCCCC AGCCCCCTTC GCCCCGAGAG 
GGCCCTCTGC CTGCTGCCCG ACCTGCTGGT GCCACTCTGG AAAGGCCCAA GACTCTCTCC 
CCAGGGAAGA ATGGGGTCGT CAAAGACGTT TTTGCCTTTG GGGGTGCCGT GGAGAACCCC 
GAGTACTTGA CACCCCAGGG AGGAGCTGCC CCTCAGCCCC ACCCTCCTCC TGCCTTCAGC 
CCAGCCTTCG ACAACCTCTA TTACTGGGAC CAGGACCCAC CAGAGCGGGG GGCTCCACCC 
AGCACCTTCA AAGGGACACC TACGGCAGAG AACCCAGAGT ACCTGGGTCT GGACGTGCCA 
GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ERBB2 ( XM_024450642.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024306410.1 Homo sapiens erb-b2 receptor tyrosine kinase 2 (ERBB2), transcript variant X2, mRNA.

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CloneID	OHu104277
Clone ID Related Accession (Same CDS sequence)	XM_024450642.1
Accession Version	XM_024450642.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3861bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	receptor tyrosine-protein kinase erbB-2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCCGGG GGTCCTGGAA GCCACAAGTG TGCACCGGCA CAGACATGAA GCTGCGGCTC 
CCTGCCAGTC CCGAGACCCA CCTGGACATG CTCCGCCACC TCTACCAGGG CTGCCAGGTG 
GTGCAGGGAA ACCTGGAACT CACCTACCTG CCCACCAATG CCAGCCTGTC CTTCCTGCAG 
GATATCCAGG AGGTGCAGGG CTACGTGCTC ATCGCTCACA ACCAAGTGAG GCAGGTCCCA 
CTGCAGAGGC TGCGGATTGT GCGAGGCACC CAGCTCTTTG AGGACAACTA TGCCCTGGCC 
GTGCTAGACA ATGGAGACCC GCTGAACAAT ACCACCCCTG TCACAGGGGC CTCCCCAGGA 
GGCCTGCGGG AGCTGCAGCT TCGAAGCCTC ACAGAGATCT TGAAAGGAGG GGTCTTGATC 
CAGCGGAACC CCCAGCTCTG CTACCAGGAC ACGATTTTGT GGAAGGACAT CTTCCACAAG 
AACAACCAGC TGGCTCTCAC ACTGATAGAC ACCAACCGCT CTCGGGCCTG CCACCCCTGT 
TCTCCGATGT GTAAGGGCTC CCGCTGCTGG GGAGAGAGTT CTGAGGATTG TCAGAGCCTG 
ACGCGCACTG TCTGTGCCGG TGGCTGTGCC CGCTGCAAGG GGCCACTGCC CACTGACTGC 
TGCCATGAGC AGTGTGCTGC CGGCTGCACG GGCCCCAAGC ACTCTGACTG CCTGGTATGT 
GCCTCTGCTT TGTGCCCAAT GTGCTCTACC CCCCAGGATG CAAGGGGTGG GCACCCTGCC 
TGGTACTGCC CTATTGCCCC TGGCACACCA GGGCAAAACA GCACAGTGAA AGCCAGCCAC 
CTGTCCCCCC AGGCCTGCCT CCACTTCAAC CACAGTGGCA TCTGTGAGCT GCACTGCCCA 
GCCCTGGTCA CCTACAACAC AGACACGTTT GAGTCCATGC CCAATCCCGA GGGCCGGTAT 
ACATTCGGCG CCAGCTGTGT GACTGCCTGT CCCTACAACT ACCTTTCTAC GGACGTGGGA 
TCCTGCACCC TCGTCTGCCC CCTGCACAAC CAAGAGGTGA CAGCAGAGGA TGGAACACAG 
CGGTGTGAGA AGTGCAGCAA GCCCTGTGCC CGAGTGTGCT ATGGTCTGGG CATGGAGCAC 
TTGCGAGAGG TGAGGGCAGT TACCAGTGCC AATATCCAGG AGTTTGCTGG CTGCAAGAAG 
ATCTTTGGGA GCCTGGCATT TCTGCCGGAG AGCTTTGATG GGGACCCAGC CTCCAACACT 
GCCCCGCTCC AGCCAGAGCA GCTCCAAGTG TTTGAGACTC TGGAAGAGAT CACAGGTTAC 
CTATACATCT CAGCATGGCC GGACAGCCTG CCTGACCTCA GCGTCTTCCA GAACCTGCAA 
GTAATCCGGG GACGAATTCT GCACAATGGC GCCTACTCGC TGACCCTGCA AGGGCTGGGC 
ATCAGCTGGC TGGGGCTGCG CTCACTGAGG GAACTGGGCA GTGGACTGGC CCTCATCCAC 
CATAACACCC ACCTCTGCTT CGTGCACACG GTGCCCTGGG ACCAGCTCTT TCGGAACCCG 
CACCAAGCTC TGCTCCACAC TGCCAACCGG CCAGAGGACG AGTGTGTGGG CGAGGGCCTG 
GCCTGCCACC AGCTGTGCGC CCGAGGGCAC TGCTGGGGTC CAGGGCCCAC CCAGTGTGTC 
AACTGCAGCC AGTTCCTTCG GGGCCAGGAG TGCGTGGAGG AATGCCGAGT ACTGCAGGGG 
CTCCCCAGGG AGTATGTGAA TGCCAGGCAC TGTTTGCCGT GCCACCCTGA GTGTCAGCCC 
CAGAATGGCT CAGTGACCTG TTTTGGACCG GAGGCTGACC AGTGTGTGGC CTGTGCCCAC 
TATAAGGACC CTCCCTTCTG CGTGGCCCGC TGCCCCAGCG GTGTGAAACC TGACCTCTCC 
TACATGCCCA TCTGGAAGTT TCCAGATGAG GAGGGCGCAT GCCAGCCTTG CCCCATCAAC 
TGCACCCACT CCTGTGTGGA CCTGGATGAC AAGGGCTGCC CCGCCGAGCA GAGAGCCAGC 
CCTCTGACGT CCATCATCTC TGCGGTGGTT GGCATTCTGC TGGTCGTGGT CTTGGGGGTG 
GTCTTTGGGA TCCTCATCAA GCGACGGCAG CAGAAGATCC GGAAGTACAC GATGCGGAGA 
CTGCTGCAGG AAACGGAGCT GGTGGAGCCG CTGACACCTA GCGGAGCGAT GCCCAACCAG 
GCGCAGATGC GGATCCTGAA AGAGACGGAG CTGAGGAAGG TGAAGGTGCT TGGATCTGGC 
GCTTTTGGCA CAGTCTACAA GGGCATCTGG ATCCCTGATG GGGAGAATGT GAAAATTCCA 
GTGGCCATCA AAGTGTTGAG GGAAAACACA TCCCCCAAAG CCAACAAAGA AATCTTAGAC 
GAAGCATACG TGATGGCTGG TGTGGGCTCC CCATATGTCT CCCGCCTTCT GGGCATCTGC 
CTGACATCCA CGGTGCAGCT GGTGACACAG CTTATGCCCT ATGGCTGCCT CTTAGACCAT 
GTCCGGGAAA ACCGCGGACG CCTGGGCTCC CAGGACCTGC TGAACTGGTG TATGCAGATT 
GCCAAGGGGA TGAGCTACCT GGAGGATGTG CGGCTCGTAC ACAGGGACTT GGCCGCTCGG 
AACGTGCTGG TCAAGAGTCC CAACCATGTC AAAATTACAG ACTTCGGGCT GGCTCGGCTG 
CTGGACATTG ACGAGACAGA GTACCATGCA GATGGGGGCA AGGTGCCCAT CAAGTGGATG 
GCGCTGGAGT CCATTCTCCG CCGGCGGTTC ACCCACCAGA GTGATGTGTG GAGTTATGGT 
GTGACTGTGT GGGAGCTGAT GACTTTTGGG GCCAAACCTT ACGATGGGAT CCCAGCCCGG 
GAGATCCCTG ACCTGCTGGA AAAGGGGGAG CGGCTGCCCC AGCCCCCCAT CTGCACCATT 
GATGTCTACA TGATCATGGT CAAATGTTGG ATGATTGACT CTGAATGTCG GCCAAGATTC 
CGGGAGTTGG TGTCTGAATT CTCCCGCATG GCCAGGGACC CCCAGCGCTT TGTGGTCATC 
CAGAATGAGG ACTTGGGCCC AGCCAGTCCC TTGGACAGCA CCTTCTACCG CTCACTGCTG 
GAGGACGATG ACATGGGGGA CCTGGTGGAT GCTGAGGAGT ATCTGGTACC CCAGCAGGGC 
TTCTTCTGTC CAGACCCTGC CCCGGGCGCT GGGGGCATGG TCCACCACAG GCACCGCAGC 
TCATCTACCA GGAGTGGCGG TGGGGACCTG ACACTAGGGC TGGAGCCCTC TGAAGAGGAG 
GCCCCCAGGT CTCCACTGGC ACCCTCCGAA GGGGCTGGCT CCGATGTATT TGATGGTGAC 
CTGGGAATGG GGGCAGCCAA GGGGCTGCAA AGCCTCCCCA CACATGACCC CAGCCCTCTA 
CAGCGGTACA GTGAGGACCC CACAGTACCC CTGCCCTCTG AGACTGATGG CTACGTTGCC 
CCCCTGACCT GCAGCCCCCA GCCTGAATAT GTGAACCAGC CAGATGTTCG GCCCCAGCCC 
CCTTCGCCCC GAGAGGGCCC TCTGCCTGCT GCCCGACCTG CTGGTGCCAC TCTGGAAAGG 
CCCAAGACTC TCTCCCCAGG GAAGAATGGG GTCGTCAAAG ACGTTTTTGC CTTTGGGGGT 
GCCGTGGAGA ACCCCGAGTA CTTGACACCC CAGGGAGGAG CTGCCCCTCA GCCCCACCCT 
CCTCCTGCCT TCAGCCCAGC CTTCGACAAC CTCTATTACT GGGACCAGGA CCCACCAGAG 
CGGGGGGCTC CACCCAGCAC CTTCAAAGGG ACACCTACGG CAGAGAACCC AGAGTACCTG 
GGTCTGGACG TGCCAGTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024306410.1
CDS	522..4382
Translation	MPRGSWKPQVCTGTDMKLRLPASPETHLDMLRHLYQGCQVVQGNLELTYLPTNASLSFLQDIQEVQGYVLIAHNQVRQVPLQRLRIVRGTQLFEDNYALAVLDNGDPLNNTTPVTGASPGGLRELQLRSLTEILKGGVLIQRNPQLCYQDTILWKDIFHKNNQLALTLIDTNRSRACHPCSPMCKGSRCWGESSEDCQSLTRTVCAGGCARCKGPLPTDCCHEQCAAGCTGPKHSDCLVCASALCPMCSTPQDARGGHPAWYCPIAPGTPGQNSTVKASHLSPQACLHFNHSGICELHCPALVTYNTDTFESMPNPEGRYTFGASCVTACPYNYLSTDVGSCTLVCPLHNQEVTAEDGTQRCEKCSKPCARVCYGLGMEHLREVRAVTSANIQEFAGCKKIFGSLAFLPESFDGDPASNTAPLQPEQLQVFETLEEITGYLYISAWPDSLPDLSVFQNLQVIRGRILHNGAYSLTLQGLGISWLGLRSLRELGSGLALIHHNTHLCFVHTVPWDQLFRNPHQALLHTANRPEDECVGEGLACHQLCARGHCWGPGPTQCVNCSQFLRGQECVEECRVLQGLPREYVNARHCLPCHPECQPQNGSVTCFGPEADQCVACAHYKDPPFCVARCPSGVKPDLSYMPIWKFPDEEGACQPCPINCTHSCVDLDDKGCPAEQRASPLTSIISAVVGILLVVVLGVVFGILIKRRQQKIRKYTMRRLLQETELVEPLTPSGAMPNQAQMRILKETELRKVKVLGSGAFGTVYKGIWIPDGENVKIPVAIKVLRENTSPKANKEILDEAYVMAGVGSPYVSRLLGICLTSTVQLVTQLMPYGCLLDHVRENRGRLGSQDLLNWCMQIAKGMSYLEDVRLVHRDLAARNVLVKSPNHVKITDFGLARLLDIDETEYHADGGKVPIKWMALESILRRRFTHQSDVWSYGVTVWELMTFGAKPYDGIPAREIPDLLEKGERLPQPPICTIDVYMIMVKCWMIDSECRPRFRELVSEFSRMARDPQRFVVIQNEDLGPASPLDSTFYRSLLEDDDMGDLVDAEEYLVPQQGFFCPDPAPGAGGMVHHRHRSSSTRSGGGDLTLGLEPSEEEAPRSPLAPSEGAGSDVFDGDLGMGAAKGLQSLPTHDPSPLQRYSEDPTVPLPSETDGYVAPLTCSPQPEYVNQPDVRPQPPSPREGPLPAARPAGATLERPKTLSPGKNGVVKDVFAFGGAVENPEYLTPQGGAAPQPHPPPAFSPAFDNLYYWDQDPPERGAPPSTFKGTPTAENPEYLGLDVPV

Target ORF information:

RefSeq Version	XM_024450642.1
Organism	Homo sapiens(human)
Definition	Homo sapiens erb-b2 receptor tyrosine kinase 2 (ERBB2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024450642.1

ORF Insert Sequence:

ATGCCCCGGG GGTCCTGGAA GCCACAAGTG TGCACCGGCA CAGACATGAA GCTGCGGCTC 
CCTGCCAGTC CCGAGACCCA CCTGGACATG CTCCGCCACC TCTACCAGGG CTGCCAGGTG 
GTGCAGGGAA ACCTGGAACT CACCTACCTG CCCACCAATG CCAGCCTGTC CTTCCTGCAG 
GATATCCAGG AGGTGCAGGG CTACGTGCTC ATCGCTCACA ACCAAGTGAG GCAGGTCCCA 
CTGCAGAGGC TGCGGATTGT GCGAGGCACC CAGCTCTTTG AGGACAACTA TGCCCTGGCC 
GTGCTAGACA ATGGAGACCC GCTGAACAAT ACCACCCCTG TCACAGGGGC CTCCCCAGGA 
GGCCTGCGGG AGCTGCAGCT TCGAAGCCTC ACAGAGATCT TGAAAGGAGG GGTCTTGATC 
CAGCGGAACC CCCAGCTCTG CTACCAGGAC ACGATTTTGT GGAAGGACAT CTTCCACAAG 
AACAACCAGC TGGCTCTCAC ACTGATAGAC ACCAACCGCT CTCGGGCCTG CCACCCCTGT 
TCTCCGATGT GTAAGGGCTC CCGCTGCTGG GGAGAGAGTT CTGAGGATTG TCAGAGCCTG 
ACGCGCACTG TCTGTGCCGG TGGCTGTGCC CGCTGCAAGG GGCCACTGCC CACTGACTGC 
TGCCATGAGC AGTGTGCTGC CGGCTGCACG GGCCCCAAGC ACTCTGACTG CCTGGTATGT 
GCCTCTGCTT TGTGCCCAAT GTGCTCTACC CCCCAGGATG CAAGGGGTGG GCACCCTGCC 
TGGTACTGCC CTATTGCCCC TGGCACACCA GGGCAAAACA GCACAGTGAA AGCCAGCCAC 
CTGTCCCCCC AGGCCTGCCT CCACTTCAAC CACAGTGGCA TCTGTGAGCT GCACTGCCCA 
GCCCTGGTCA CCTACAACAC AGACACGTTT GAGTCCATGC CCAATCCCGA GGGCCGGTAT 
ACATTCGGCG CCAGCTGTGT GACTGCCTGT CCCTACAACT ACCTTTCTAC GGACGTGGGA 
TCCTGCACCC TCGTCTGCCC CCTGCACAAC CAAGAGGTGA CAGCAGAGGA TGGAACACAG 
CGGTGTGAGA AGTGCAGCAA GCCCTGTGCC CGAGTGTGCT ATGGTCTGGG CATGGAGCAC 
TTGCGAGAGG TGAGGGCAGT TACCAGTGCC AATATCCAGG AGTTTGCTGG CTGCAAGAAG 
ATCTTTGGGA GCCTGGCATT TCTGCCGGAG AGCTTTGATG GGGACCCAGC CTCCAACACT 
GCCCCGCTCC AGCCAGAGCA GCTCCAAGTG TTTGAGACTC TGGAAGAGAT CACAGGTTAC 
CTATACATCT CAGCATGGCC GGACAGCCTG CCTGACCTCA GCGTCTTCCA GAACCTGCAA 
GTAATCCGGG GACGAATTCT GCACAATGGC GCCTACTCGC TGACCCTGCA AGGGCTGGGC 
ATCAGCTGGC TGGGGCTGCG CTCACTGAGG GAACTGGGCA GTGGACTGGC CCTCATCCAC 
CATAACACCC ACCTCTGCTT CGTGCACACG GTGCCCTGGG ACCAGCTCTT TCGGAACCCG 
CACCAAGCTC TGCTCCACAC TGCCAACCGG CCAGAGGACG AGTGTGTGGG CGAGGGCCTG 
GCCTGCCACC AGCTGTGCGC CCGAGGGCAC TGCTGGGGTC CAGGGCCCAC CCAGTGTGTC 
AACTGCAGCC AGTTCCTTCG GGGCCAGGAG TGCGTGGAGG AATGCCGAGT ACTGCAGGGG 
CTCCCCAGGG AGTATGTGAA TGCCAGGCAC TGTTTGCCGT GCCACCCTGA GTGTCAGCCC 
CAGAATGGCT CAGTGACCTG TTTTGGACCG GAGGCTGACC AGTGTGTGGC CTGTGCCCAC 
TATAAGGACC CTCCCTTCTG CGTGGCCCGC TGCCCCAGCG GTGTGAAACC TGACCTCTCC 
TACATGCCCA TCTGGAAGTT TCCAGATGAG GAGGGCGCAT GCCAGCCTTG CCCCATCAAC 
TGCACCCACT CCTGTGTGGA CCTGGATGAC AAGGGCTGCC CCGCCGAGCA GAGAGCCAGC 
CCTCTGACGT CCATCATCTC TGCGGTGGTT GGCATTCTGC TGGTCGTGGT CTTGGGGGTG 
GTCTTTGGGA TCCTCATCAA GCGACGGCAG CAGAAGATCC GGAAGTACAC GATGCGGAGA 
CTGCTGCAGG AAACGGAGCT GGTGGAGCCG CTGACACCTA GCGGAGCGAT GCCCAACCAG 
GCGCAGATGC GGATCCTGAA AGAGACGGAG CTGAGGAAGG TGAAGGTGCT TGGATCTGGC 
GCTTTTGGCA CAGTCTACAA GGGCATCTGG ATCCCTGATG GGGAGAATGT GAAAATTCCA 
GTGGCCATCA AAGTGTTGAG GGAAAACACA TCCCCCAAAG CCAACAAAGA AATCTTAGAC 
GAAGCATACG TGATGGCTGG TGTGGGCTCC CCATATGTCT CCCGCCTTCT GGGCATCTGC 
CTGACATCCA CGGTGCAGCT GGTGACACAG CTTATGCCCT ATGGCTGCCT CTTAGACCAT 
GTCCGGGAAA ACCGCGGACG CCTGGGCTCC CAGGACCTGC TGAACTGGTG TATGCAGATT 
GCCAAGGGGA TGAGCTACCT GGAGGATGTG CGGCTCGTAC ACAGGGACTT GGCCGCTCGG 
AACGTGCTGG TCAAGAGTCC CAACCATGTC AAAATTACAG ACTTCGGGCT GGCTCGGCTG 
CTGGACATTG ACGAGACAGA GTACCATGCA GATGGGGGCA AGGTGCCCAT CAAGTGGATG 
GCGCTGGAGT CCATTCTCCG CCGGCGGTTC ACCCACCAGA GTGATGTGTG GAGTTATGGT 
GTGACTGTGT GGGAGCTGAT GACTTTTGGG GCCAAACCTT ACGATGGGAT CCCAGCCCGG 
GAGATCCCTG ACCTGCTGGA AAAGGGGGAG CGGCTGCCCC AGCCCCCCAT CTGCACCATT 
GATGTCTACA TGATCATGGT CAAATGTTGG ATGATTGACT CTGAATGTCG GCCAAGATTC 
CGGGAGTTGG TGTCTGAATT CTCCCGCATG GCCAGGGACC CCCAGCGCTT TGTGGTCATC 
CAGAATGAGG ACTTGGGCCC AGCCAGTCCC TTGGACAGCA CCTTCTACCG CTCACTGCTG 
GAGGACGATG ACATGGGGGA CCTGGTGGAT GCTGAGGAGT ATCTGGTACC CCAGCAGGGC 
TTCTTCTGTC CAGACCCTGC CCCGGGCGCT GGGGGCATGG TCCACCACAG GCACCGCAGC 
TCATCTACCA GGAGTGGCGG TGGGGACCTG ACACTAGGGC TGGAGCCCTC TGAAGAGGAG 
GCCCCCAGGT CTCCACTGGC ACCCTCCGAA GGGGCTGGCT CCGATGTATT TGATGGTGAC 
CTGGGAATGG GGGCAGCCAA GGGGCTGCAA AGCCTCCCCA CACATGACCC CAGCCCTCTA 
CAGCGGTACA GTGAGGACCC CACAGTACCC CTGCCCTCTG AGACTGATGG CTACGTTGCC 
CCCCTGACCT GCAGCCCCCA GCCTGAATAT GTGAACCAGC CAGATGTTCG GCCCCAGCCC 
CCTTCGCCCC GAGAGGGCCC TCTGCCTGCT GCCCGACCTG CTGGTGCCAC TCTGGAAAGG 
CCCAAGACTC TCTCCCCAGG GAAGAATGGG GTCGTCAAAG ACGTTTTTGC CTTTGGGGGT 
GCCGTGGAGA ACCCCGAGTA CTTGACACCC CAGGGAGGAG CTGCCCCTCA GCCCCACCCT 
CCTCCTGCCT TCAGCCCAGC CTTCGACAAC CTCTATTACT GGGACCAGGA CCCACCAGAG 
CGGGGGGCTC CACCCAGCAC CTTCAAAGGG ACACCTACGG CAGAGAACCC AGAGTACCTG 
GGTCTGGACG TGCCAGTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ERBB2 ( XM_024450643.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024306411.1 Homo sapiens erb-b2 receptor tyrosine kinase 2 (ERBB2), transcript variant X3, mRNA.

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CloneID	OHu104278
Clone ID Related Accession (Same CDS sequence)	XM_024450643.1
Accession Version	XM_024450643.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3816bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	receptor tyrosine-protein kinase erbB-2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCTGC GGCTCCCTGC CAGTCCCGAG ACCCACCTGG ACATGCTCCG CCACCTCTAC 
CAGGGCTGCC AGGTGGTGCA GGGAAACCTG GAACTCACCT ACCTGCCCAC CAATGCCAGC 
CTGTCCTTCC TGCAGGATAT CCAGGAGGTG CAGGGCTACG TGCTCATCGC TCACAACCAA 
GTGAGGCAGG TCCCACTGCA GAGGCTGCGG ATTGTGCGAG GCACCCAGCT CTTTGAGGAC 
AACTATGCCC TGGCCGTGCT AGACAATGGA GACCCGCTGA ACAATACCAC CCCTGTCACA 
GGGGCCTCCC CAGGAGGCCT GCGGGAGCTG CAGCTTCGAA GCCTCACAGA GATCTTGAAA 
GGAGGGGTCT TGATCCAGCG GAACCCCCAG CTCTGCTACC AGGACACGAT TTTGTGGAAG 
GACATCTTCC ACAAGAACAA CCAGCTGGCT CTCACACTGA TAGACACCAA CCGCTCTCGG 
GCCTGCCACC CCTGTTCTCC GATGTGTAAG GGCTCCCGCT GCTGGGGAGA GAGTTCTGAG 
GATTGTCAGA GCCTGACGCG CACTGTCTGT GCCGGTGGCT GTGCCCGCTG CAAGGGGCCA 
CTGCCCACTG ACTGCTGCCA TGAGCAGTGT GCTGCCGGCT GCACGGGCCC CAAGCACTCT 
GACTGCCTGG TATGTGCCTC TGCTTTGTGC CCAATGTGCT CTACCCCCCA GGATGCAAGG 
GGTGGGCACC CTGCCTGGTA CTGCCCTATT GCCCCTGGCA CACCAGGGCA AAACAGCACA 
GTGAAAGCCA GCCACCTGTC CCCCCAGGCC TGCCTCCACT TCAACCACAG TGGCATCTGT 
GAGCTGCACT GCCCAGCCCT GGTCACCTAC AACACAGACA CGTTTGAGTC CATGCCCAAT 
CCCGAGGGCC GGTATACATT CGGCGCCAGC TGTGTGACTG CCTGTCCCTA CAACTACCTT 
TCTACGGACG TGGGATCCTG CACCCTCGTC TGCCCCCTGC ACAACCAAGA GGTGACAGCA 
GAGGATGGAA CACAGCGGTG TGAGAAGTGC AGCAAGCCCT GTGCCCGAGT GTGCTATGGT 
CTGGGCATGG AGCACTTGCG AGAGGTGAGG GCAGTTACCA GTGCCAATAT CCAGGAGTTT 
GCTGGCTGCA AGAAGATCTT TGGGAGCCTG GCATTTCTGC CGGAGAGCTT TGATGGGGAC 
CCAGCCTCCA ACACTGCCCC GCTCCAGCCA GAGCAGCTCC AAGTGTTTGA GACTCTGGAA 
GAGATCACAG GTTACCTATA CATCTCAGCA TGGCCGGACA GCCTGCCTGA CCTCAGCGTC 
TTCCAGAACC TGCAAGTAAT CCGGGGACGA ATTCTGCACA ATGGCGCCTA CTCGCTGACC 
CTGCAAGGGC TGGGCATCAG CTGGCTGGGG CTGCGCTCAC TGAGGGAACT GGGCAGTGGA 
CTGGCCCTCA TCCACCATAA CACCCACCTC TGCTTCGTGC ACACGGTGCC CTGGGACCAG 
CTCTTTCGGA ACCCGCACCA AGCTCTGCTC CACACTGCCA ACCGGCCAGA GGACGAGTGT 
GTGGGCGAGG GCCTGGCCTG CCACCAGCTG TGCGCCCGAG GGCACTGCTG GGGTCCAGGG 
CCCACCCAGT GTGTCAACTG CAGCCAGTTC CTTCGGGGCC AGGAGTGCGT GGAGGAATGC 
CGAGTACTGC AGGGGCTCCC CAGGGAGTAT GTGAATGCCA GGCACTGTTT GCCGTGCCAC 
CCTGAGTGTC AGCCCCAGAA TGGCTCAGTG ACCTGTTTTG GACCGGAGGC TGACCAGTGT 
GTGGCCTGTG CCCACTATAA GGACCCTCCC TTCTGCGTGG CCCGCTGCCC CAGCGGTGTG 
AAACCTGACC TCTCCTACAT GCCCATCTGG AAGTTTCCAG ATGAGGAGGG CGCATGCCAG 
CCTTGCCCCA TCAACTGCAC CCACTCCTGT GTGGACCTGG ATGACAAGGG CTGCCCCGCC 
GAGCAGAGAG CCAGCCCTCT GACGTCCATC ATCTCTGCGG TGGTTGGCAT TCTGCTGGTC 
GTGGTCTTGG GGGTGGTCTT TGGGATCCTC ATCAAGCGAC GGCAGCAGAA GATCCGGAAG 
TACACGATGC GGAGACTGCT GCAGGAAACG GAGCTGGTGG AGCCGCTGAC ACCTAGCGGA 
GCGATGCCCA ACCAGGCGCA GATGCGGATC CTGAAAGAGA CGGAGCTGAG GAAGGTGAAG 
GTGCTTGGAT CTGGCGCTTT TGGCACAGTC TACAAGGGCA TCTGGATCCC TGATGGGGAG 
AATGTGAAAA TTCCAGTGGC CATCAAAGTG TTGAGGGAAA ACACATCCCC CAAAGCCAAC 
AAAGAAATCT TAGACGAAGC ATACGTGATG GCTGGTGTGG GCTCCCCATA TGTCTCCCGC 
CTTCTGGGCA TCTGCCTGAC ATCCACGGTG CAGCTGGTGA CACAGCTTAT GCCCTATGGC 
TGCCTCTTAG ACCATGTCCG GGAAAACCGC GGACGCCTGG GCTCCCAGGA CCTGCTGAAC 
TGGTGTATGC AGATTGCCAA GGGGATGAGC TACCTGGAGG ATGTGCGGCT CGTACACAGG 
GACTTGGCCG CTCGGAACGT GCTGGTCAAG AGTCCCAACC ATGTCAAAAT TACAGACTTC 
GGGCTGGCTC GGCTGCTGGA CATTGACGAG ACAGAGTACC ATGCAGATGG GGGCAAGGTG 
CCCATCAAGT GGATGGCGCT GGAGTCCATT CTCCGCCGGC GGTTCACCCA CCAGAGTGAT 
GTGTGGAGTT ATGGTGTGAC TGTGTGGGAG CTGATGACTT TTGGGGCCAA ACCTTACGAT 
GGGATCCCAG CCCGGGAGAT CCCTGACCTG CTGGAAAAGG GGGAGCGGCT GCCCCAGCCC 
CCCATCTGCA CCATTGATGT CTACATGATC ATGGTCAAAT GTTGGATGAT TGACTCTGAA 
TGTCGGCCAA GATTCCGGGA GTTGGTGTCT GAATTCTCCC GCATGGCCAG GGACCCCCAG 
CGCTTTGTGG TCATCCAGAA TGAGGACTTG GGCCCAGCCA GTCCCTTGGA CAGCACCTTC 
TACCGCTCAC TGCTGGAGGA CGATGACATG GGGGACCTGG TGGATGCTGA GGAGTATCTG 
GTACCCCAGC AGGGCTTCTT CTGTCCAGAC CCTGCCCCGG GCGCTGGGGG CATGGTCCAC 
CACAGGCACC GCAGCTCATC TACCAGGAGT GGCGGTGGGG ACCTGACACT AGGGCTGGAG 
CCCTCTGAAG AGGAGGCCCC CAGGTCTCCA CTGGCACCCT CCGAAGGGGC TGGCTCCGAT 
GTATTTGATG GTGACCTGGG AATGGGGGCA GCCAAGGGGC TGCAAAGCCT CCCCACACAT 
GACCCCAGCC CTCTACAGCG GTACAGTGAG GACCCCACAG TACCCCTGCC CTCTGAGACT 
GATGGCTACG TTGCCCCCCT GACCTGCAGC CCCCAGCCTG AATATGTGAA CCAGCCAGAT 
GTTCGGCCCC AGCCCCCTTC GCCCCGAGAG GGCCCTCTGC CTGCTGCCCG ACCTGCTGGT 
GCCACTCTGG AAAGGCCCAA GACTCTCTCC CCAGGGAAGA ATGGGGTCGT CAAAGACGTT 
TTTGCCTTTG GGGGTGCCGT GGAGAACCCC GAGTACTTGA CACCCCAGGG AGGAGCTGCC 
CCTCAGCCCC ACCCTCCTCC TGCCTTCAGC CCAGCCTTCG ACAACCTCTA TTACTGGGAC 
CAGGACCCAC CAGAGCGGGG GGCTCCACCC AGCACCTTCA AAGGGACACC TACGGCAGAG 
AACCCAGAGT ACCTGGGTCT GGACGTGCCA GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024306411.1
CDS	564..4379
Translation	MKLRLPASPETHLDMLRHLYQGCQVVQGNLELTYLPTNASLSFLQDIQEVQGYVLIAHNQVRQVPLQRLRIVRGTQLFEDNYALAVLDNGDPLNNTTPVTGASPGGLRELQLRSLTEILKGGVLIQRNPQLCYQDTILWKDIFHKNNQLALTLIDTNRSRACHPCSPMCKGSRCWGESSEDCQSLTRTVCAGGCARCKGPLPTDCCHEQCAAGCTGPKHSDCLVCASALCPMCSTPQDARGGHPAWYCPIAPGTPGQNSTVKASHLSPQACLHFNHSGICELHCPALVTYNTDTFESMPNPEGRYTFGASCVTACPYNYLSTDVGSCTLVCPLHNQEVTAEDGTQRCEKCSKPCARVCYGLGMEHLREVRAVTSANIQEFAGCKKIFGSLAFLPESFDGDPASNTAPLQPEQLQVFETLEEITGYLYISAWPDSLPDLSVFQNLQVIRGRILHNGAYSLTLQGLGISWLGLRSLRELGSGLALIHHNTHLCFVHTVPWDQLFRNPHQALLHTANRPEDECVGEGLACHQLCARGHCWGPGPTQCVNCSQFLRGQECVEECRVLQGLPREYVNARHCLPCHPECQPQNGSVTCFGPEADQCVACAHYKDPPFCVARCPSGVKPDLSYMPIWKFPDEEGACQPCPINCTHSCVDLDDKGCPAEQRASPLTSIISAVVGILLVVVLGVVFGILIKRRQQKIRKYTMRRLLQETELVEPLTPSGAMPNQAQMRILKETELRKVKVLGSGAFGTVYKGIWIPDGENVKIPVAIKVLRENTSPKANKEILDEAYVMAGVGSPYVSRLLGICLTSTVQLVTQLMPYGCLLDHVRENRGRLGSQDLLNWCMQIAKGMSYLEDVRLVHRDLAARNVLVKSPNHVKITDFGLARLLDIDETEYHADGGKVPIKWMALESILRRRFTHQSDVWSYGVTVWELMTFGAKPYDGIPAREIPDLLEKGERLPQPPICTIDVYMIMVKCWMIDSECRPRFRELVSEFSRMARDPQRFVVIQNEDLGPASPLDSTFYRSLLEDDDMGDLVDAEEYLVPQQGFFCPDPAPGAGGMVHHRHRSSSTRSGGGDLTLGLEPSEEEAPRSPLAPSEGAGSDVFDGDLGMGAAKGLQSLPTHDPSPLQRYSEDPTVPLPSETDGYVAPLTCSPQPEYVNQPDVRPQPPSPREGPLPAARPAGATLERPKTLSPGKNGVVKDVFAFGGAVENPEYLTPQGGAAPQPHPPPAFSPAFDNLYYWDQDPPERGAPPSTFKGTPTAENPEYLGLDVPV

Target ORF information:

RefSeq Version	XM_024450643.1
Organism	Homo sapiens(human)
Definition	Homo sapiens erb-b2 receptor tyrosine kinase 2 (ERBB2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024450643.1

ORF Insert Sequence:

ATGAAGCTGC GGCTCCCTGC CAGTCCCGAG ACCCACCTGG ACATGCTCCG CCACCTCTAC 
CAGGGCTGCC AGGTGGTGCA GGGAAACCTG GAACTCACCT ACCTGCCCAC CAATGCCAGC 
CTGTCCTTCC TGCAGGATAT CCAGGAGGTG CAGGGCTACG TGCTCATCGC TCACAACCAA 
GTGAGGCAGG TCCCACTGCA GAGGCTGCGG ATTGTGCGAG GCACCCAGCT CTTTGAGGAC 
AACTATGCCC TGGCCGTGCT AGACAATGGA GACCCGCTGA ACAATACCAC CCCTGTCACA 
GGGGCCTCCC CAGGAGGCCT GCGGGAGCTG CAGCTTCGAA GCCTCACAGA GATCTTGAAA 
GGAGGGGTCT TGATCCAGCG GAACCCCCAG CTCTGCTACC AGGACACGAT TTTGTGGAAG 
GACATCTTCC ACAAGAACAA CCAGCTGGCT CTCACACTGA TAGACACCAA CCGCTCTCGG 
GCCTGCCACC CCTGTTCTCC GATGTGTAAG GGCTCCCGCT GCTGGGGAGA GAGTTCTGAG 
GATTGTCAGA GCCTGACGCG CACTGTCTGT GCCGGTGGCT GTGCCCGCTG CAAGGGGCCA 
CTGCCCACTG ACTGCTGCCA TGAGCAGTGT GCTGCCGGCT GCACGGGCCC CAAGCACTCT 
GACTGCCTGG TATGTGCCTC TGCTTTGTGC CCAATGTGCT CTACCCCCCA GGATGCAAGG 
GGTGGGCACC CTGCCTGGTA CTGCCCTATT GCCCCTGGCA CACCAGGGCA AAACAGCACA 
GTGAAAGCCA GCCACCTGTC CCCCCAGGCC TGCCTCCACT TCAACCACAG TGGCATCTGT 
GAGCTGCACT GCCCAGCCCT GGTCACCTAC AACACAGACA CGTTTGAGTC CATGCCCAAT 
CCCGAGGGCC GGTATACATT CGGCGCCAGC TGTGTGACTG CCTGTCCCTA CAACTACCTT 
TCTACGGACG TGGGATCCTG CACCCTCGTC TGCCCCCTGC ACAACCAAGA GGTGACAGCA 
GAGGATGGAA CACAGCGGTG TGAGAAGTGC AGCAAGCCCT GTGCCCGAGT GTGCTATGGT 
CTGGGCATGG AGCACTTGCG AGAGGTGAGG GCAGTTACCA GTGCCAATAT CCAGGAGTTT 
GCTGGCTGCA AGAAGATCTT TGGGAGCCTG GCATTTCTGC CGGAGAGCTT TGATGGGGAC 
CCAGCCTCCA ACACTGCCCC GCTCCAGCCA GAGCAGCTCC AAGTGTTTGA GACTCTGGAA 
GAGATCACAG GTTACCTATA CATCTCAGCA TGGCCGGACA GCCTGCCTGA CCTCAGCGTC 
TTCCAGAACC TGCAAGTAAT CCGGGGACGA ATTCTGCACA ATGGCGCCTA CTCGCTGACC 
CTGCAAGGGC TGGGCATCAG CTGGCTGGGG CTGCGCTCAC TGAGGGAACT GGGCAGTGGA 
CTGGCCCTCA TCCACCATAA CACCCACCTC TGCTTCGTGC ACACGGTGCC CTGGGACCAG 
CTCTTTCGGA ACCCGCACCA AGCTCTGCTC CACACTGCCA ACCGGCCAGA GGACGAGTGT 
GTGGGCGAGG GCCTGGCCTG CCACCAGCTG TGCGCCCGAG GGCACTGCTG GGGTCCAGGG 
CCCACCCAGT GTGTCAACTG CAGCCAGTTC CTTCGGGGCC AGGAGTGCGT GGAGGAATGC 
CGAGTACTGC AGGGGCTCCC CAGGGAGTAT GTGAATGCCA GGCACTGTTT GCCGTGCCAC 
CCTGAGTGTC AGCCCCAGAA TGGCTCAGTG ACCTGTTTTG GACCGGAGGC TGACCAGTGT 
GTGGCCTGTG CCCACTATAA GGACCCTCCC TTCTGCGTGG CCCGCTGCCC CAGCGGTGTG 
AAACCTGACC TCTCCTACAT GCCCATCTGG AAGTTTCCAG ATGAGGAGGG CGCATGCCAG 
CCTTGCCCCA TCAACTGCAC CCACTCCTGT GTGGACCTGG ATGACAAGGG CTGCCCCGCC 
GAGCAGAGAG CCAGCCCTCT GACGTCCATC ATCTCTGCGG TGGTTGGCAT TCTGCTGGTC 
GTGGTCTTGG GGGTGGTCTT TGGGATCCTC ATCAAGCGAC GGCAGCAGAA GATCCGGAAG 
TACACGATGC GGAGACTGCT GCAGGAAACG GAGCTGGTGG AGCCGCTGAC ACCTAGCGGA 
GCGATGCCCA ACCAGGCGCA GATGCGGATC CTGAAAGAGA CGGAGCTGAG GAAGGTGAAG 
GTGCTTGGAT CTGGCGCTTT TGGCACAGTC TACAAGGGCA TCTGGATCCC TGATGGGGAG 
AATGTGAAAA TTCCAGTGGC CATCAAAGTG TTGAGGGAAA ACACATCCCC CAAAGCCAAC 
AAAGAAATCT TAGACGAAGC ATACGTGATG GCTGGTGTGG GCTCCCCATA TGTCTCCCGC 
CTTCTGGGCA TCTGCCTGAC ATCCACGGTG CAGCTGGTGA CACAGCTTAT GCCCTATGGC 
TGCCTCTTAG ACCATGTCCG GGAAAACCGC GGACGCCTGG GCTCCCAGGA CCTGCTGAAC 
TGGTGTATGC AGATTGCCAA GGGGATGAGC TACCTGGAGG ATGTGCGGCT CGTACACAGG 
GACTTGGCCG CTCGGAACGT GCTGGTCAAG AGTCCCAACC ATGTCAAAAT TACAGACTTC 
GGGCTGGCTC GGCTGCTGGA CATTGACGAG ACAGAGTACC ATGCAGATGG GGGCAAGGTG 
CCCATCAAGT GGATGGCGCT GGAGTCCATT CTCCGCCGGC GGTTCACCCA CCAGAGTGAT 
GTGTGGAGTT ATGGTGTGAC TGTGTGGGAG CTGATGACTT TTGGGGCCAA ACCTTACGAT 
GGGATCCCAG CCCGGGAGAT CCCTGACCTG CTGGAAAAGG GGGAGCGGCT GCCCCAGCCC 
CCCATCTGCA CCATTGATGT CTACATGATC ATGGTCAAAT GTTGGATGAT TGACTCTGAA 
TGTCGGCCAA GATTCCGGGA GTTGGTGTCT GAATTCTCCC GCATGGCCAG GGACCCCCAG 
CGCTTTGTGG TCATCCAGAA TGAGGACTTG GGCCCAGCCA GTCCCTTGGA CAGCACCTTC 
TACCGCTCAC TGCTGGAGGA CGATGACATG GGGGACCTGG TGGATGCTGA GGAGTATCTG 
GTACCCCAGC AGGGCTTCTT CTGTCCAGAC CCTGCCCCGG GCGCTGGGGG CATGGTCCAC 
CACAGGCACC GCAGCTCATC TACCAGGAGT GGCGGTGGGG ACCTGACACT AGGGCTGGAG 
CCCTCTGAAG AGGAGGCCCC CAGGTCTCCA CTGGCACCCT CCGAAGGGGC TGGCTCCGAT 
GTATTTGATG GTGACCTGGG AATGGGGGCA GCCAAGGGGC TGCAAAGCCT CCCCACACAT 
GACCCCAGCC CTCTACAGCG GTACAGTGAG GACCCCACAG TACCCCTGCC CTCTGAGACT 
GATGGCTACG TTGCCCCCCT GACCTGCAGC CCCCAGCCTG AATATGTGAA CCAGCCAGAT 
GTTCGGCCCC AGCCCCCTTC GCCCCGAGAG GGCCCTCTGC CTGCTGCCCG ACCTGCTGGT 
GCCACTCTGG AAAGGCCCAA GACTCTCTCC CCAGGGAAGA ATGGGGTCGT CAAAGACGTT 
TTTGCCTTTG GGGGTGCCGT GGAGAACCCC GAGTACTTGA CACCCCAGGG AGGAGCTGCC 
CCTCAGCCCC ACCCTCCTCC TGCCTTCAGC CCAGCCTTCG ACAACCTCTA TTACTGGGAC 
CAGGACCCAC CAGAGCGGGG GGCTCCACCC AGCACCTTCA AAGGGACACC TACGGCAGAG 
AACCCAGAGT ACCTGGGTCT GGACGTGCCA GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ERBB2 ( NM_001289937.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001276866.1 Homo sapiens erb-b2 receptor tyrosine kinase 2 (ERBB2), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OHu24035
Clone ID Related Accession (Same CDS sequence)	NM_001289937.1
Accession Version	NM_001289937.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3168bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Homo sapiens(human)
Product	receptor tyrosine-protein kinase erbB-2 isoform d precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC346286.1, AK313683.1, BC080193.1 and BM678576.1. Transcript Variant: This variant (4) lacks an exon in the 3' coding region, which results in a translational frameshift, compared to variant 1. The resulting isoform (d) has a distinct and shorter C-terminus, compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK313683.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145245, SAMEA2147596 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## regulatory uORF :: PMID: 10446211 ##RefSeq-Attributes-END##

ATGGAGCTGG CGGCCTTGTG CCGCTGGGGG CTCCTCCTCG CCCTCTTGCC CCCCGGAGCC 
GCGAGCACCC AAGTGTGCAC CGGCACAGAC ATGAAGCTGC GGCTCCCTGC CAGTCCCGAG 
ACCCACCTGG ACATGCTCCG CCACCTCTAC CAGGGCTGCC AGGTGGTGCA GGGAAACCTG 
GAACTCACCT ACCTGCCCAC CAATGCCAGC CTGTCCTTCC TGCAGGATAT CCAGGAGGTG 
CAGGGCTACG TGCTCATCGC TCACAACCAA GTGAGGCAGG TCCCACTGCA GAGGCTGCGG 
ATTGTGCGAG GCACCCAGCT CTTTGAGGAC AACTATGCCC TGGCCGTGCT AGACAATGGA 
GACCCGCTGA ACAATACCAC CCCTGTCACA GGGGCCTCCC CAGGAGGCCT GCGGGAGCTG 
CAGCTTCGAA GCCTCACAGA GATCTTGAAA GGAGGGGTCT TGATCCAGCG GAACCCCCAG 
CTCTGCTACC AGGACACGAT TTTGTGGAAG GACATCTTCC ACAAGAACAA CCAGCTGGCT 
CTCACACTGA TAGACACCAA CCGCTCTCGG GCCTGCCACC CCTGTTCTCC GATGTGTAAG 
GGCTCCCGCT GCTGGGGAGA GAGTTCTGAG GATTGTCAGA GCCTGACGCG CACTGTCTGT 
GCCGGTGGCT GTGCCCGCTG CAAGGGGCCA CTGCCCACTG ACTGCTGCCA TGAGCAGTGT 
GCTGCCGGCT GCACGGGCCC CAAGCACTCT GACTGCCTGG CCTGCCTCCA CTTCAACCAC 
AGTGGCATCT GTGAGCTGCA CTGCCCAGCC CTGGTCACCT ACAACACAGA CACGTTTGAG 
TCCATGCCCA ATCCCGAGGG CCGGTATACA TTCGGCGCCA GCTGTGTGAC TGCCTGTCCC 
TACAACTACC TTTCTACGGA CGTGGGATCC TGCACCCTCG TCTGCCCCCT GCACAACCAA 
GAGGTGACAG CAGAGGATGG AACACAGCGG TGTGAGAAGT GCAGCAAGCC CTGTGCCCGA 
GTGTGCTATG GTCTGGGCAT GGAGCACTTG CGAGAGGTGA GGGCAGTTAC CAGTGCCAAT 
ATCCAGGAGT TTGCTGGCTG CAAGAAGATC TTTGGGAGCC TGGCATTTCT GCCGGAGAGC 
TTTGATGGGG ACCCAGCCTC CAACACTGCC CCGCTCCAGC CAGAGCAGCT CCAAGTGTTT 
GAGACTCTGG AAGAGATCAC AGGTTACCTA TACATCTCAG CATGGCCGGA CAGCCTGCCT 
GACCTCAGCG TCTTCCAGAA CCTGCAAGTA ATCCGGGGAC GAATTCTGCA CAATGGCGCC 
TACTCGCTGA CCCTGCAAGG GCTGGGCATC AGCTGGCTGG GGCTGCGCTC ACTGAGGGAA 
CTGGGCAGTG GACTGGCCCT CATCCACCAT AACACCCACC TCTGCTTCGT GCACACGGTG 
CCCTGGGACC AGCTCTTTCG GAACCCGCAC CAAGCTCTGC TCCACACTGC CAACCGGCCA 
GAGGACGAGT GTGTGGGCGA GGGCCTGGCC TGCCACCAGC TGTGCGCCCG AGGGCACTGC 
TGGGGTCCAG GGCCCACCCA GTGTGTCAAC TGCAGCCAGT TCCTTCGGGG CCAGGAGTGC 
GTGGAGGAAT GCCGAGTACT GCAGGGGCTC CCCAGGGAGT ATGTGAATGC CAGGCACTGT 
TTGCCGTGCC ACCCTGAGTG TCAGCCCCAG AATGGCTCAG TGACCTGTTT TGGACCGGAG 
GCTGACCAGT GTGTGGCCTG TGCCCACTAT AAGGACCCTC CCTTCTGCGT GGCCCGCTGC 
CCCAGCGGTG TGAAACCTGA CCTCTCCTAC ATGCCCATCT GGAAGTTTCC AGATGAGGAG 
GGCGCATGCC AGCCTTGCCC CATCAACTGC ACCCACTCCT GTGTGGACCT GGATGACAAG 
GGCTGCCCCG CCGAGCAGAG AGCCAGCCCT CTGACGTCCA TCATCTCTGC GGTGGTTGGC 
ATTCTGCTGG TCGTGGTCTT GGGGGTGGTC TTTGGGATCC TCATCAAGCG ACGGCAGCAG 
AAGATCCGGA AGTACACGAT GCGGAGACTG CTGCAGGAAA CGGAGCTGGT GGAGCCGCTG 
ACACCTAGCG GAGCGATGCC CAACCAGGCG CAGATGCGGA TCCTGAAAGA GACGGAGCTG 
AGGAAGGTGA AGGTGCTTGG ATCTGGCGCT TTTGGCACAG TCTACAAGGG CATCTGGATC 
CCTGATGGGG AGAATGTGAA AATTCCAGTG GCCATCAAAG TGTTGAGGGA AAACACATCC 
CCCAAAGCCA ACAAAGAAAT CTTAGACGAA GCATACGTGA TGGCTGGTGT GGGCTCCCCA 
TATGTCTCCC GCCTTCTGGG CATCTGCCTG ACATCCACGG TGCAGCTGGT GACACAGCTT 
ATGCCCTATG GCTGCCTCTT AGACCATGTC CGGGAAAACC GCGGACGCCT GGGCTCCCAG 
GACCTGCTGA ACTGGTGTAT GCAGATTGCC AAGGGGATGA GCTACCTGGA GGATGTGCGG 
CTCGTACACA GGGACTTGGC CGCTCGGAAC GTGCTGGTCA AGAGTCCCAA CCATGTCAAA 
ATTACAGACT TCGGGCTGGC TCGGCTGCTG GACATTGACG AGACAGAGTA CCATGCAGAT 
GGGGGCAAGG TGCCCATCAA GTGGATGGCG CTGGAGTCCA TTCTCCGCCG GCGGTTCACC 
CACCAGAGTG ATGTGTGGAG TTATGGTGTG ACTGTGTGGG AGCTGATGAC TTTTGGGGCC 
AAACCTTACG ATGGGATCCC AGCCCGGGAG ATCCCTGACC TGCTGGAAAA GGGGGAGCGG 
CTGCCCCAGC CCCCCATCTG CACCATTGAT GTCTACATGA TCATGGTCAA ATGTTGGATG 
ATTGACTCTG AATGTCGGCC AAGATTCCGG GAGTTGGTGT CTGAATTCTC CCGCATGGCC 
AGGGACCCCC AGCGCTTTGT GGTCATCCAG AATGAGGACT TGGGCCCAGC CAGTCCCTTG 
GACAGCACCT TCTACCGCTC ACTGCTGGAG GACGATGACA TGGGGGACCT GGTGGATGCT 
GAGGAGTATC TGGTACCCCA GCAGGGCTTC TTCTGTCCAG ACCCTGCCCC GGGCGCTGGG 
GGCATGGTCC ACCACAGGCA CCGCAGCTCA TCTACCAGGA ATATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001276866.1
CDS	262..3429
Translation	MELAALCRWGLLLALLPPGAASTQVCTGTDMKLRLPASPETHLDMLRHLYQGCQVVQGNLELTYLPTNASLSFLQDIQEVQGYVLIAHNQVRQVPLQRLRIVRGTQLFEDNYALAVLDNGDPLNNTTPVTGASPGGLRELQLRSLTEILKGGVLIQRNPQLCYQDTILWKDIFHKNNQLALTLIDTNRSRACHPCSPMCKGSRCWGESSEDCQSLTRTVCAGGCARCKGPLPTDCCHEQCAAGCTGPKHSDCLACLHFNHSGICELHCPALVTYNTDTFESMPNPEGRYTFGASCVTACPYNYLSTDVGSCTLVCPLHNQEVTAEDGTQRCEKCSKPCARVCYGLGMEHLREVRAVTSANIQEFAGCKKIFGSLAFLPESFDGDPASNTAPLQPEQLQVFETLEEITGYLYISAWPDSLPDLSVFQNLQVIRGRILHNGAYSLTLQGLGISWLGLRSLRELGSGLALIHHNTHLCFVHTVPWDQLFRNPHQALLHTANRPEDECVGEGLACHQLCARGHCWGPGPTQCVNCSQFLRGQECVEECRVLQGLPREYVNARHCLPCHPECQPQNGSVTCFGPEADQCVACAHYKDPPFCVARCPSGVKPDLSYMPIWKFPDEEGACQPCPINCTHSCVDLDDKGCPAEQRASPLTSIISAVVGILLVVVLGVVFGILIKRRQQKIRKYTMRRLLQETELVEPLTPSGAMPNQAQMRILKETELRKVKVLGSGAFGTVYKGIWIPDGENVKIPVAIKVLRENTSPKANKEILDEAYVMAGVGSPYVSRLLGICLTSTVQLVTQLMPYGCLLDHVRENRGRLGSQDLLNWCMQIAKGMSYLEDVRLVHRDLAARNVLVKSPNHVKITDFGLARLLDIDETEYHADGGKVPIKWMALESILRRRFTHQSDVWSYGVTVWELMTFGAKPYDGIPAREIPDLLEKGERLPQPPICTIDVYMIMVKCWMIDSECRPRFRELVSEFSRMARDPQRFVVIQNEDLGPASPLDSTFYRSLLEDDDMGDLVDAEEYLVPQQGFFCPDPAPGAGGMVHHRHRSSSTRNM

Target ORF information:

RefSeq Version	NM_001289937.1
Organism	Homo sapiens(human)
Definition	Homo sapiens erb-b2 receptor tyrosine kinase 2 (ERBB2), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001289937.1

ORF Insert Sequence:

ATGGAGCTGG CGGCCTTGTG CCGCTGGGGG CTCCTCCTCG CCCTCTTGCC CCCCGGAGCC 
GCGAGCACCC AAGTGTGCAC CGGCACAGAC ATGAAGCTGC GGCTCCCTGC CAGTCCCGAG 
ACCCACCTGG ACATGCTCCG CCACCTCTAC CAGGGCTGCC AGGTGGTGCA GGGAAACCTG 
GAACTCACCT ACCTGCCCAC CAATGCCAGC CTGTCCTTCC TGCAGGATAT CCAGGAGGTG 
CAGGGCTACG TGCTCATCGC TCACAACCAA GTGAGGCAGG TCCCACTGCA GAGGCTGCGG 
ATTGTGCGAG GCACCCAGCT CTTTGAGGAC AACTATGCCC TGGCCGTGCT AGACAATGGA 
GACCCGCTGA ACAATACCAC CCCTGTCACA GGGGCCTCCC CAGGAGGCCT GCGGGAGCTG 
CAGCTTCGAA GCCTCACAGA GATCTTGAAA GGAGGGGTCT TGATCCAGCG GAACCCCCAG 
CTCTGCTACC AGGACACGAT TTTGTGGAAG GACATCTTCC ACAAGAACAA CCAGCTGGCT 
CTCACACTGA TAGACACCAA CCGCTCTCGG GCCTGCCACC CCTGTTCTCC GATGTGTAAG 
GGCTCCCGCT GCTGGGGAGA GAGTTCTGAG GATTGTCAGA GCCTGACGCG CACTGTCTGT 
GCCGGTGGCT GTGCCCGCTG CAAGGGGCCA CTGCCCACTG ACTGCTGCCA TGAGCAGTGT 
GCTGCCGGCT GCACGGGCCC CAAGCACTCT GACTGCCTGG CCTGCCTCCA CTTCAACCAC 
AGTGGCATCT GTGAGCTGCA CTGCCCAGCC CTGGTCACCT ACAACACAGA CACGTTTGAG 
TCCATGCCCA ATCCCGAGGG CCGGTATACA TTCGGCGCCA GCTGTGTGAC TGCCTGTCCC 
TACAACTACC TTTCTACGGA CGTGGGATCC TGCACCCTCG TCTGCCCCCT GCACAACCAA 
GAGGTGACAG CAGAGGATGG AACACAGCGG TGTGAGAAGT GCAGCAAGCC CTGTGCCCGA 
GTGTGCTATG GTCTGGGCAT GGAGCACTTG CGAGAGGTGA GGGCAGTTAC CAGTGCCAAT 
ATCCAGGAGT TTGCTGGCTG CAAGAAGATC TTTGGGAGCC TGGCATTTCT GCCGGAGAGC 
TTTGATGGGG ACCCAGCCTC CAACACTGCC CCGCTCCAGC CAGAGCAGCT CCAAGTGTTT 
GAGACTCTGG AAGAGATCAC AGGTTACCTA TACATCTCAG CATGGCCGGA CAGCCTGCCT 
GACCTCAGCG TCTTCCAGAA CCTGCAAGTA ATCCGGGGAC GAATTCTGCA CAATGGCGCC 
TACTCGCTGA CCCTGCAAGG GCTGGGCATC AGCTGGCTGG GGCTGCGCTC ACTGAGGGAA 
CTGGGCAGTG GACTGGCCCT CATCCACCAT AACACCCACC TCTGCTTCGT GCACACGGTG 
CCCTGGGACC AGCTCTTTCG GAACCCGCAC CAAGCTCTGC TCCACACTGC CAACCGGCCA 
GAGGACGAGT GTGTGGGCGA GGGCCTGGCC TGCCACCAGC TGTGCGCCCG AGGGCACTGC 
TGGGGTCCAG GGCCCACCCA GTGTGTCAAC TGCAGCCAGT TCCTTCGGGG CCAGGAGTGC 
GTGGAGGAAT GCCGAGTACT GCAGGGGCTC CCCAGGGAGT ATGTGAATGC CAGGCACTGT 
TTGCCGTGCC ACCCTGAGTG TCAGCCCCAG AATGGCTCAG TGACCTGTTT TGGACCGGAG 
GCTGACCAGT GTGTGGCCTG TGCCCACTAT AAGGACCCTC CCTTCTGCGT GGCCCGCTGC 
CCCAGCGGTG TGAAACCTGA CCTCTCCTAC ATGCCCATCT GGAAGTTTCC AGATGAGGAG 
GGCGCATGCC AGCCTTGCCC CATCAACTGC ACCCACTCCT GTGTGGACCT GGATGACAAG 
GGCTGCCCCG CCGAGCAGAG AGCCAGCCCT CTGACGTCCA TCATCTCTGC GGTGGTTGGC 
ATTCTGCTGG TCGTGGTCTT GGGGGTGGTC TTTGGGATCC TCATCAAGCG ACGGCAGCAG 
AAGATCCGGA AGTACACGAT GCGGAGACTG CTGCAGGAAA CGGAGCTGGT GGAGCCGCTG 
ACACCTAGCG GAGCGATGCC CAACCAGGCG CAGATGCGGA TCCTGAAAGA GACGGAGCTG 
AGGAAGGTGA AGGTGCTTGG ATCTGGCGCT TTTGGCACAG TCTACAAGGG CATCTGGATC 
CCTGATGGGG AGAATGTGAA AATTCCAGTG GCCATCAAAG TGTTGAGGGA AAACACATCC 
CCCAAAGCCA ACAAAGAAAT CTTAGACGAA GCATACGTGA TGGCTGGTGT GGGCTCCCCA 
TATGTCTCCC GCCTTCTGGG CATCTGCCTG ACATCCACGG TGCAGCTGGT GACACAGCTT 
ATGCCCTATG GCTGCCTCTT AGACCATGTC CGGGAAAACC GCGGACGCCT GGGCTCCCAG 
GACCTGCTGA ACTGGTGTAT GCAGATTGCC AAGGGGATGA GCTACCTGGA GGATGTGCGG 
CTCGTACACA GGGACTTGGC CGCTCGGAAC GTGCTGGTCA AGAGTCCCAA CCATGTCAAA 
ATTACAGACT TCGGGCTGGC TCGGCTGCTG GACATTGACG AGACAGAGTA CCATGCAGAT 
GGGGGCAAGG TGCCCATCAA GTGGATGGCG CTGGAGTCCA TTCTCCGCCG GCGGTTCACC 
CACCAGAGTG ATGTGTGGAG TTATGGTGTG ACTGTGTGGG AGCTGATGAC TTTTGGGGCC 
AAACCTTACG ATGGGATCCC AGCCCGGGAG ATCCCTGACC TGCTGGAAAA GGGGGAGCGG 
CTGCCCCAGC CCCCCATCTG CACCATTGAT GTCTACATGA TCATGGTCAA ATGTTGGATG 
ATTGACTCTG AATGTCGGCC AAGATTCCGG GAGTTGGTGT CTGAATTCTC CCGCATGGCC 
AGGGACCCCC AGCGCTTTGT GGTCATCCAG AATGAGGACT TGGGCCCAGC CAGTCCCTTG 
GACAGCACCT TCTACCGCTC ACTGCTGGAG GACGATGACA TGGGGGACCT GGTGGATGCT 
GAGGAGTATC TGGTACCCCA GCAGGGCTTC TTCTGTCCAG ACCCTGCCCC GGGCGCTGGG 
GGCATGGTCC ACCACAGGCA CCGCAGCTCA TCTACCAGGA ATATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Translational control by an upstream open reading frame in the HER-2/neu transcript.
The Journal of biological chemistry274(34)24335-41(1999 Aug)
Child SJ,Miller MK,Geballe AP

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ERBB2 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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