EPRS cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	EPRS
Entrez Gene ID	2058
Full Name	glutamyl-prolyl-tRNA synthetase
Synonyms	EARS,GLUPRORS,PARS,PIG32,QARS,QPRS
General protein information	Preferred Names glutamyl-prolyl-tRNA synthetase Names bifunctional glutamate/proline--tRNA ligase bifunctional aminoacyl-tRNA synthetase cell proliferation-inducing gene 32 protein glutamate tRNA ligase glutamatyl-prolyl-tRNA synthetase glutaminyl-tRNA synthetase proliferation-inducing gene 32 protein proliferation-inducing protein 32 proline-tRNA ligase prolyl-tRNA synthetase
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 1 Map Location: 1q41
Summary	Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. The protein encoded by this gene is a multifunctional aminoacyl-tRNA synthetase that catalyzes the aminoacylation of glutamic acid and proline tRNA species. Alternative splicing has been observed for this gene, but the full-length nature and biological validity of the variant have not been determined. [provided by RefSeq, Jul 2008].
Disorder MIM:	138295

mRNA and Protein(s)

mRNA	Protein	Name
NM_004446.3	NP_004437.2	bifunctional glutamate/proline--tRNA ligase
XM_017000614.2	XP_016856103.1	bifunctional glutamate/proline--tRNA ligase isoform X1
NM_004446.2	NP_004437.2	bifunctional glutamate/proline--tRNA ligase
XM_017000614.1	XP_016856103.1	bifunctional glutamate/proline--tRNA ligase isoform X1

Pathways from BioSystems

Homologies

Saccharomyces cerevisiae (baker's yeast)	GUS1	NP_011269.2
Arabidopsis thaliana (thale cress)	AT5G26710	NP_850874.1
Xenopus tropicalis (tropical clawed frog)	eprs	XP_002936064.2
Pan troglodytes (chimpanzee)	EPRS	XP_001172425.1
	LOC706899	XP_002808297.1
Bos taurus (cattle)	EPRS	NP_001230249.1
Danio rerio (zebrafish)	eprs	NP_001191177.1
Canis lupus familiaris (dog)	EPRS	XP_005640882.1
Magnaporthe oryzae (rice blast fungus)	MGG_05956	XP_003711794.1
Neurospora crassa	NCU08894	XP_958832.1
Kluyveromyces lactis	KLLA0A00682g	XP_451028.1
Eremothecium gossypii	AGOS_AFR264W	NP_985811.2
Schizosaccharomyces pombe (fission yeast)	SPAC17A5.15c	NP_593483.1
Oryza sativa (rice)	Os10g0369000	NP_001064451.1
Homo sapiens (human)	EPRS	NP_004437.2
Mus musculus (house mouse)	Eprs	NP_084011.1
Rattus norvegicus (Norway rat)	Eprs	NP_001019409.1
Oryza sativa (rice)	Os01g0271200	NP_001042706.1

Gene Ontology
Bibliography

Related articles in PubMed

A Single Adaptable Cochaperone-Scaffold Complex Delivers Nascent Iron-Sulfur Clusters to Mammalian Respiratory Chain Complexes I-III.
Maio N, Kim KS, Singh A, Rouault TA
Cell metabolism25(4)945-953.e6(2017 Apr)

EPRS is a critical mTORC1-S6K1 effector that influences adiposity in mice.
Arif A, Terenzi F, Potdar AA, Jia J, Sacks J, China A, Halawani D, Vasu K, Li X, Brown JM, Chen J, Kozma SC, Thomas G, Fox PL
Nature542(7641)357-361(2017 02)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

analysis of the heterotetrameric complex structure of the glutathione transferase (GST) domains shared among the four MSC components, methionyl-tRNA synthetase (MRS), glutaminyl-prolyl-tRNA synthetase (EPRS), AIMP2 and AIMP3
Title: Assembly of Multi-tRNA Synthetase Complex via Heterotetrameric Glutathione Transferase-homology Domains.

Dynamic model simulations predicted an inhibitory GAIT-element-interacting factor to account for this relationship and led to the identification of a truncated form of EPRS, a GAIT constituent that mediates binding to target transcripts.
Title: Coding region polyadenylation generates a truncated tRNA synthetase that counters translation repression.

Study reveals a unique role of Cdk5/p35 in activation of the major noncanonical function of EPRS, namely translational control of macrophage inflammatory gene expression.
Title: Phosphorylation of glutamyl-prolyl tRNA synthetase by cyclin-dependent kinase 5 dictates transcript-selective translational control.

Clinical trial of gene-disease association and gene-environment interaction. (HuGE Navigator)
Title: Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score.

EPRS phosphorylation events regulate GAIT-mediated gene silencing.
Title: Two-site phosphorylation of EPRS coordinates multimodal regulation of noncanonical translational control activity.

The following EPRS gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EPRS cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu04348	NM_004446.3 Latest version!	Homo sapiens glutamyl-prolyl-tRNA synthetase 1 (EPRS1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$274.50-$384.30 $549.00
OHu83326	XM_017000614.2 Latest version!	Homo sapiens glutamyl-prolyl-tRNA synthetase 1 (EPRS1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
View Old Accession Versions >>
OHu04348	NM_004446.2	Homo sapiens glutamyl-prolyl-tRNA synthetase (EPRS), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$274.50-$384.30 $549.00
OHu83326	XM_017000614.1	Homo sapiens glutamyl-prolyl-tRNA synthetase (EPRS), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

EPRS ( NM_004446.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_004437.2 Homo sapiens glutamyl-prolyl-tRNA synthetase 1 (EPRS1), mRNA.

Price & Availability↑

CloneID	OHu04348
Clone ID Related Accession (Same CDS sequence)	NM_004446.3 , NM_004446.2
Accession Version	NM_004446.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4539bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-25
Organism	Homo sapiens(human)
Product	bifunctional glutamate/proline--tRNA ligase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC046156.1, BC034797.1, AY493416.1, N85252.1 and AA196283.1. On Nov 23, 2018 this sequence version replaced NM_004446.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.14153.1, SRR1803613.34075.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000366923.8/ ENSP00000355890.3 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCGACGC TCTCTCTGAC CGTGAATTCA GGAGACCCTC CGCTAGGAGC TTTGCTGGCA 
GTAGAACACG TGAAAGACGA TGTCAGCATT TCCGTTGAAG AAGGGAAAGA GAATATTCTT 
CATGTTTCTG AAAATGTGAT ATTCACAGAT GTGAATTCTA TACTTCGCTA CTTGGCTAGA 
GTTGCAACTA CAGCTGGGTT ATATGGCTCT AATCTGATGG AACATACTGA GATTGATCAC 
TGGTTGGAGT TCAGTGCTAC AAAATTATCT TCATGTGATT CCTTTACTTC TACAATTAAT 
GAACTCAATC ATTGCCTGTC TCTGAGAACA TACTTAGTTG GAAACTCCTT GAGTTTAGCA 
GATTTATGTG TTTGGGCCAC CCTAAAAGGA AATGCTGCCT GGCAAGAACA GTTGAAACAG 
AAGAAAGCTC CAGTTCATGT AAAACGTTGG TTTGGCTTTC TTGAAGCCCA GCAGGCCTTC 
CAGTCAGTAG GTACCAAGTG GGATGTTTCA ACAACCAAAG CTCGAGTGGC ACCTGAGAAA 
AAGCAAGATG TTGGGAAATT TGTTGAGCTT CCAGGTGCGG AGATGGGAAA GGTTACCGTC 
AGATTTCCTC CAGAGGCCAG TGGTTACTTA CACATTGGGC ATGCAAAAGC TGCTCTTCTG 
AACCAGCACT ACCAGGTTAA CTTTAAAGGG AAACTGATCA TGAGATTTGA TGACACAAAT 
CCTGAAAAAG AAAAGGAAGA TTTTGAGAAG GTTATCTTGG AAGATGTTGC AATGTTGCAT 
ATCAAACCAG ATCAATTTAC TTATACTTCG GATCATTTTG AAACTATAAT GAAGTATGCA 
GAGAAGCTAA TTCAAGAAGG GAAGGCTTAT GTGGATGATA CTCCTGCTGA ACAGATGAAA 
GCAGAACGTG AGCAGAGGAT AGACTCTAAA CATAGAAAAA ACCCTATTGA GAAGAATCTA 
CAAATGTGGG AAGAAATGAA AAAAGGGAGC CAGTTTGGTC AGTCCTGTTG TTTGCGAGCA 
AAAATTGACA TGAGTAGTAA CAATGGATGC ATGAGAGATC CAACCCTTTA TCGCTGCAAA 
ATTCAACCAC ATCCAAGAAC TGGAAATAAA TACAATGTTT ATCCAACATA TGATTTTGCC 
TGCCCCATAG TTGACAGCAT CGAAGGTGTT ACACATGCCC TGAGAACAAC AGAATACCAT 
GACAGAGATG AGCAGTTTTA CTGGATTATT GAAGCTTTAG GCATAAGAAA ACCATATATT 
TGGGAATATA GTCGGCTAAA TCTCAACAAC ACAGTGCTAT CCAAAAGAAA ACTCACATGG 
TTTGTCAATG AAGGACTAGT AGATGGATGG GATGACCCAA GATTTCCTAC GGTTCGTGGT 
GTACTGAGAA GAGGGATGAC AGTTGAAGGA CTGAAACAGT TTATTGCTGC TCAGGGCTCC 
TCACGTTCAG TCGTGAACAT GGAGTGGGAC AAAATCTGGG CGTTTAACAA AAAGGTTATT 
GACCCAGTGG CTCCACGATA TGTTGCATTA CTGAAGAAAG AAGTGATCCC AGTGAATGTA 
CCTGAAGCTC AGGAGGAGAT GAAAGAAGTA GCCAAACACC CAAAGAATCC TGAGGTTGGC 
TTGAAGCCTG TGTGGTATAG TCCCAAAGTT TTCATTGAAG GTGCTGATGC AGAGACTTTT 
TCGGAGGGTG AGATGGTTAC ATTTATAAAT TGGGGCAACC TCAACATTAC AAAAATACAC 
AAAAATGCAG ATGGAAAAAT CATATCTCTT GATGCAAAGT TGAATTTGGA AAACAAAGAC 
TACAAGAAAA CCACTAAGGT CACTTGGCTT GCAGAGACTA CACATGCTCT TCCTATTCCA 
GTAATCTGTG TCACTTATGA GCACTTGATC ACAAAGCCAG TGCTAGGAAA AGACGAGGAC 
TTTAAGCAGT ATGTCAACAA GAACAGTAAG CATGAAGAGC TAATGCTAGG GGATCCCTGC 
CTTAAGGATT TGAAAAAAGG AGATATTATA CAACTCCAGA GAAGAGGATT CTTCATATGT 
GATCAACCTT ATGAACCTGT TAGCCCATAT AGTTGCAAGG AAGCCCCGTG TGTTTTGATA 
TACATTCCTG ATGGGCACAC AAAGGAAATG CCAACATCAG GGTCAAAGGA AAAGACCAAA 
GTAGAAGCCA CAAAAAATGA GACCTCTGCT CCTTTTAAGG AAAGACCAAC ACCTTCTCTG 
AATAATAATT GTACTACATC TGAGGATTCC TTGGTCCTTT ACAATAGAGT GGCTGTTCAA 
GGAGATGTGG TTCGTGAATT AAAAGCCAAG AAAGCACCAA AGGAAGATGT AGATGCAGCT 
GTAAAACAGC TTTTGTCTTT GAAAGCTGAA TATAAGGAGA AAACTGGCCA GGAATATAAA 
CCTGGAAACC CTCCTGCTGA AATAGGACAG AATATTTCTT CTAATTCCTC AGCAAGTATT 
CTGGAAAGTA AATCTCTGTA TGATGAAGTT GCTGCACAAG GGGAGGTGGT TCGTAAGCTA 
AAAGCTGAAA AATCCCCTAA GGCTAAAATA AATGAAGCTG TAGAATGCTT ACTGTCCCTG 
AAGGCTCAGT ATAAAGAAAA AACTGGGAAG GAGTACATAC CTGGTCAGCC CCCATTATCT 
CAAAGTTCGG ATTCAAGCCC AACCAGAAAT TCTGAACCTG CTGGTTTAGA AACACCAGAA 
GCGAAAGTAC TTTTTGACAA AGTAGCTTCT CAAGGGGAAG TAGTTCGGAA ACTTAAAACT 
GAAAAAGCCC CTAAGGATCA AGTAGATATA GCTGTTCAAG AACTCCTTCA GCTAAAGGCA 
CAGTACAAGT CTTTGATAGG AGTAGAGTAT AAGCCTGTGT CGGCCACTGG AGCTGAGGAC 
AAAGATAAGA AGAAGAAAGA AAAAGAAAAT AAATCTGAAA AGCAGAATAA GCCTCAGAAA 
CAAAATGATG GCCAAAGGAA AGACCCTTCT AAAAACCAAG GAGGTGGGCT CTCATCAAGT 
GGAGCAGGAG AAGGGCAGGG GCCTAAGAAA CAGACCAGGT TGGGTCTTGA GGCAAAAAAA 
GAAGAAAATC TTGCTGATTG GTATTCTCAG GTCATCACAA AGTCAGAAAT GATTGAATAC 
CATGACATAA GTGGCTGTTA TATTCTTCGT CCCTGGGCCT ATGCCATTTG GGAAGCCATC 
AAGGACTTTT TTGATGCTGA GATCAAGAAA CTTGGTGTTG AAAACTGCTA CTTCCCCATG 
TTTGTGTCTC AAAGTGCATT AGAGAAAGAG AAGACTCATG TTGCTGACTT TGCCCCAGAG 
GTTGCTTGGG TTACAAGATC TGGCAAAACC GAGCTGGCAG AACCAATTGC CATTCGTCCT 
ACTAGTGAAA CAGTAATGTA TCCTGCATAT GCAAAATGGG TACAGTCACA CAGAGACCTG 
CCCATCAAGC TCAATCAGTG GTGCAATGTG GTGCGTTGGG AATTCAAGCA TCCTCAGCCT 
TTCCTACGTA CTCGTGAATT TCTTTGGCAG GAAGGGCACA GTGCTTTTGC TACCATGGAA 
GAGGCAGCGG AAGAGGTCTT GCAGATACTT GACTTATATG CTCAGGTATA TGAAGAACTC 
CTGGCAATTC CTGTTGTTAA AGGAAGAAAG ACGGAAAAGG AAAAATTTGC AGGAGGAGAC 
TATACAACTA CAATAGAAGC ATTTATATCT GCTAGTGGAA GAGCTATCCA GGGAGGAACA 
TCACATCATT TAGGGCAGAA TTTTTCCAAA ATGTTTGAAA TCGTTTTTGA AGATCCAAAG 
ATACCAGGAG AGAAGCAATT TGCCTATCAA AACTCCTGGG GCCTGACAAC TCGAACTATT 
GGTGTTATGA CCATGGTTCA TGGGGACAAC ATGGGTTTAG TATTACCACC CCGTGTAGCA 
TGTGTTCAGG TGGTGATTAT TCCTTGTGGC ATTACCAATG CACTTTCTGA AGAAGACAAA 
GAAGCGCTGA TTGCAAAATG CAATGATTAT CGAAGGCGAT TACTCAGTGT TAACATCCGC 
GTTAGAGCTG ATTTACGAGA TAATTATTCT CCAGGTTGGA AATTCAATCA CTGGGAGCTC 
AAGGGAGTTC CCATTAGACT TGAAGTTGGG CCACGTGATA TGAAGAGCTG TCAGTTTGTA 
GCCGTCAGAC GAGATACTGG AGAAAAGCTG ACAGTTGCTG AAAATGAGGC AGAGACTAAA 
CTTCAAGCTA TTTTGGAAGA CATCCAGGTC ACCCTTTTCA CAAGGGCTTC TGAAGACCTT 
AAGACTCATA TGGTTGTGGC TAATACAATG GAAGACTTTC AGAAGATACT AGATTCTGGA 
AAGATTGTTC AGATTCCATT CTGTGGGGAA ATTGACTGTG AGGACTGGAT CAAAAAGACC 
ACTGCCAGGG ATCAAGATCT TGAACCTGGT GCTCCATCCA TGGGAGCTAA AAGCCTTTGC 
ATCCCCTTCA AACCACTCTG TGAACTGCAG CCTGGAGCCA AATGTGTCTG TGGCAAGAAC 
CCTGCCAAGT ACTACACCTT ATTTGGTCGC AGCTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004437.2
CDS	118..4656
Translation	MATLSLTVNSGDPPLGALLAVEHVKDDVSISVEEGKENILHVSENVIFTDVNSILRYLARVATTAGLYGSNLMEHTEIDHWLEFSATKLSSCDSFTSTINELNHCLSLRTYLVGNSLSLADLCVWATLKGNAAWQEQLKQKKAPVHVKRWFGFLEAQQAFQSVGTKWDVSTTKARVAPEKKQDVGKFVELPGAEMGKVTVRFPPEASGYLHIGHAKAALLNQHYQVNFKGKLIMRFDDTNPEKEKEDFEKVILEDVAMLHIKPDQFTYTSDHFETIMKYAEKLIQEGKAYVDDTPAEQMKAEREQRIDSKHRKNPIEKNLQMWEEMKKGSQFGQSCCLRAKIDMSSNNGCMRDPTLYRCKIQPHPRTGNKYNVYPTYDFACPIVDSIEGVTHALRTTEYHDRDEQFYWIIEALGIRKPYIWEYSRLNLNNTVLSKRKLTWFVNEGLVDGWDDPRFPTVRGVLRRGMTVEGLKQFIAAQGSSRSVVNMEWDKIWAFNKKVIDPVAPRYVALLKKEVIPVNVPEAQEEMKEVAKHPKNPEVGLKPVWYSPKVFIEGADAETFSEGEMVTFINWGNLNITKIHKNADGKIISLDAKLNLENKDYKKTTKVTWLAETTHALPIPVICVTYEHLITKPVLGKDEDFKQYVNKNSKHEELMLGDPCLKDLKKGDIIQLQRRGFFICDQPYEPVSPYSCKEAPCVLIYIPDGHTKEMPTSGSKEKTKVEATKNETSAPFKERPTPSLNNNCTTSEDSLVLYNRVAVQGDVVRELKAKKAPKEDVDAAVKQLLSLKAEYKEKTGQEYKPGNPPAEIGQNISSNSSASILESKSLYDEVAAQGEVVRKLKAEKSPKAKINEAVECLLSLKAQYKEKTGKEYIPGQPPLSQSSDSSPTRNSEPAGLETPEAKVLFDKVASQGEVVRKLKTEKAPKDQVDIAVQELLQLKAQYKSLIGVEYKPVSATGAEDKDKKKKEKENKSEKQNKPQKQNDGQRKDPSKNQGGGLSSSGAGEGQGPKKQTRLGLEAKKEENLADWYSQVITKSEMIEYHDISGCYILRPWAYAIWEAIKDFFDAEIKKLGVENCYFPMFVSQSALEKEKTHVADFAPEVAWVTRSGKTELAEPIAIRPTSETVMYPAYAKWVQSHRDLPIKLNQWCNVVRWEFKHPQPFLRTREFLWQEGHSAFATMEEAAEEVLQILDLYAQVYEELLAIPVVKGRKTEKEKFAGGDYTTTIEAFISASGRAIQGGTSHHLGQNFSKMFEIVFEDPKIPGEKQFAYQNSWGLTTRTIGVMTMVHGDNMGLVLPPRVACVQVVIIPCGITNALSEEDKEALIAKCNDYRRRLLSVNIRVRADLRDNYSPGWKFNHWELKGVPIRLEVGPRDMKSCQFVAVRRDTGEKLTVAENEAETKLQAILEDIQVTLFTRASEDLKTHMVVANTMEDFQKILDSGKIVQIPFCGEIDCEDWIKKTTARDQDLEPGAPSMGAKSLCIPFKPLCELQPGAKCVCGKNPAKYYTLFGRSY

Target ORF information:

RefSeq Version	NM_004446.3
Organism	Homo sapiens(human)
Definition	Homo sapiens glutamyl-prolyl-tRNA synthetase 1 (EPRS1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004446.3

ORF Insert Sequence:

ATGGCGACGC TCTCTCTGAC CGTGAATTCA GGAGACCCTC CGCTAGGAGC TTTGCTGGCA 
GTAGAACACG TGAAAGACGA TGTCAGCATT TCCGTTGAAG AAGGGAAAGA GAATATTCTT 
CATGTTTCTG AAAATGTGAT ATTCACAGAT GTGAATTCTA TACTTCGCTA CTTGGCTAGA 
GTTGCAACTA CAGCTGGGTT ATATGGCTCT AATCTGATGG AACATACTGA GATTGATCAC 
TGGTTGGAGT TCAGTGCTAC AAAATTATCT TCATGTGATT CCTTTACTTC TACAATTAAT 
GAACTCAATC ATTGCCTGTC TCTGAGAACA TACTTAGTTG GAAACTCCTT GAGTTTAGCA 
GATTTATGTG TTTGGGCCAC CCTAAAAGGA AATGCTGCCT GGCAAGAACA GTTGAAACAG 
AAGAAAGCTC CAGTTCATGT AAAACGTTGG TTTGGCTTTC TTGAAGCCCA GCAGGCCTTC 
CAGTCAGTAG GTACCAAGTG GGATGTTTCA ACAACCAAAG CTCGAGTGGC ACCTGAGAAA 
AAGCAAGATG TTGGGAAATT TGTTGAGCTT CCAGGTGCGG AGATGGGAAA GGTTACCGTC 
AGATTTCCTC CAGAGGCCAG TGGTTACTTA CACATTGGGC ATGCAAAAGC TGCTCTTCTG 
AACCAGCACT ACCAGGTTAA CTTTAAAGGG AAACTGATCA TGAGATTTGA TGACACAAAT 
CCTGAAAAAG AAAAGGAAGA TTTTGAGAAG GTTATCTTGG AAGATGTTGC AATGTTGCAT 
ATCAAACCAG ATCAATTTAC TTATACTTCG GATCATTTTG AAACTATAAT GAAGTATGCA 
GAGAAGCTAA TTCAAGAAGG GAAGGCTTAT GTGGATGATA CTCCTGCTGA ACAGATGAAA 
GCAGAACGTG AGCAGAGGAT AGACTCTAAA CATAGAAAAA ACCCTATTGA GAAGAATCTA 
CAAATGTGGG AAGAAATGAA AAAAGGGAGC CAGTTTGGTC AGTCCTGTTG TTTGCGAGCA 
AAAATTGACA TGAGTAGTAA CAATGGATGC ATGAGAGATC CAACCCTTTA TCGCTGCAAA 
ATTCAACCAC ATCCAAGAAC TGGAAATAAA TACAATGTTT ATCCAACATA TGATTTTGCC 
TGCCCCATAG TTGACAGCAT CGAAGGTGTT ACACATGCCC TGAGAACAAC AGAATACCAT 
GACAGAGATG AGCAGTTTTA CTGGATTATT GAAGCTTTAG GCATAAGAAA ACCATATATT 
TGGGAATATA GTCGGCTAAA TCTCAACAAC ACAGTGCTAT CCAAAAGAAA ACTCACATGG 
TTTGTCAATG AAGGACTAGT AGATGGATGG GATGACCCAA GATTTCCTAC GGTTCGTGGT 
GTACTGAGAA GAGGGATGAC AGTTGAAGGA CTGAAACAGT TTATTGCTGC TCAGGGCTCC 
TCACGTTCAG TCGTGAACAT GGAGTGGGAC AAAATCTGGG CGTTTAACAA AAAGGTTATT 
GACCCAGTGG CTCCACGATA TGTTGCATTA CTGAAGAAAG AAGTGATCCC AGTGAATGTA 
CCTGAAGCTC AGGAGGAGAT GAAAGAAGTA GCCAAACACC CAAAGAATCC TGAGGTTGGC 
TTGAAGCCTG TGTGGTATAG TCCCAAAGTT TTCATTGAAG GTGCTGATGC AGAGACTTTT 
TCGGAGGGTG AGATGGTTAC ATTTATAAAT TGGGGCAACC TCAACATTAC AAAAATACAC 
AAAAATGCAG ATGGAAAAAT CATATCTCTT GATGCAAAGT TGAATTTGGA AAACAAAGAC 
TACAAGAAAA CCACTAAGGT CACTTGGCTT GCAGAGACTA CACATGCTCT TCCTATTCCA 
GTAATCTGTG TCACTTATGA GCACTTGATC ACAAAGCCAG TGCTAGGAAA AGACGAGGAC 
TTTAAGCAGT ATGTCAACAA GAACAGTAAG CATGAAGAGC TAATGCTAGG GGATCCCTGC 
CTTAAGGATT TGAAAAAAGG AGATATTATA CAACTCCAGA GAAGAGGATT CTTCATATGT 
GATCAACCTT ATGAACCTGT TAGCCCATAT AGTTGCAAGG AAGCCCCGTG TGTTTTGATA 
TACATTCCTG ATGGGCACAC AAAGGAAATG CCAACATCAG GGTCAAAGGA AAAGACCAAA 
GTAGAAGCCA CAAAAAATGA GACCTCTGCT CCTTTTAAGG AAAGACCAAC ACCTTCTCTG 
AATAATAATT GTACTACATC TGAGGATTCC TTGGTCCTTT ACAATAGAGT GGCTGTTCAA 
GGAGATGTGG TTCGTGAATT AAAAGCCAAG AAAGCACCAA AGGAAGATGT AGATGCAGCT 
GTAAAACAGC TTTTGTCTTT GAAAGCTGAA TATAAGGAGA AAACTGGCCA GGAATATAAA 
CCTGGAAACC CTCCTGCTGA AATAGGACAG AATATTTCTT CTAATTCCTC AGCAAGTATT 
CTGGAAAGTA AATCTCTGTA TGATGAAGTT GCTGCACAAG GGGAGGTGGT TCGTAAGCTA 
AAAGCTGAAA AATCCCCTAA GGCTAAAATA AATGAAGCTG TAGAATGCTT ACTGTCCCTG 
AAGGCTCAGT ATAAAGAAAA AACTGGGAAG GAGTACATAC CTGGTCAGCC CCCATTATCT 
CAAAGTTCGG ATTCAAGCCC AACCAGAAAT TCTGAACCTG CTGGTTTAGA AACACCAGAA 
GCGAAAGTAC TTTTTGACAA AGTAGCTTCT CAAGGGGAAG TAGTTCGGAA ACTTAAAACT 
GAAAAAGCCC CTAAGGATCA AGTAGATATA GCTGTTCAAG AACTCCTTCA GCTAAAGGCA 
CAGTACAAGT CTTTGATAGG AGTAGAGTAT AAGCCTGTGT CGGCCACTGG AGCTGAGGAC 
AAAGATAAGA AGAAGAAAGA AAAAGAAAAT AAATCTGAAA AGCAGAATAA GCCTCAGAAA 
CAAAATGATG GCCAAAGGAA AGACCCTTCT AAAAACCAAG GAGGTGGGCT CTCATCAAGT 
GGAGCAGGAG AAGGGCAGGG GCCTAAGAAA CAGACCAGGT TGGGTCTTGA GGCAAAAAAA 
GAAGAAAATC TTGCTGATTG GTATTCTCAG GTCATCACAA AGTCAGAAAT GATTGAATAC 
CATGACATAA GTGGCTGTTA TATTCTTCGT CCCTGGGCCT ATGCCATTTG GGAAGCCATC 
AAGGACTTTT TTGATGCTGA GATCAAGAAA CTTGGTGTTG AAAACTGCTA CTTCCCCATG 
TTTGTGTCTC AAAGTGCATT AGAGAAAGAG AAGACTCATG TTGCTGACTT TGCCCCAGAG 
GTTGCTTGGG TTACAAGATC TGGCAAAACC GAGCTGGCAG AACCAATTGC CATTCGTCCT 
ACTAGTGAAA CAGTAATGTA TCCTGCATAT GCAAAATGGG TACAGTCACA CAGAGACCTG 
CCCATCAAGC TCAATCAGTG GTGCAATGTG GTGCGTTGGG AATTCAAGCA TCCTCAGCCT 
TTCCTACGTA CTCGTGAATT TCTTTGGCAG GAAGGGCACA GTGCTTTTGC TACCATGGAA 
GAGGCAGCGG AAGAGGTCTT GCAGATACTT GACTTATATG CTCAGGTATA TGAAGAACTC 
CTGGCAATTC CTGTTGTTAA AGGAAGAAAG ACGGAAAAGG AAAAATTTGC AGGAGGAGAC 
TATACAACTA CAATAGAAGC ATTTATATCT GCTAGTGGAA GAGCTATCCA GGGAGGAACA 
TCACATCATT TAGGGCAGAA TTTTTCCAAA ATGTTTGAAA TCGTTTTTGA AGATCCAAAG 
ATACCAGGAG AGAAGCAATT TGCCTATCAA AACTCCTGGG GCCTGACAAC TCGAACTATT 
GGTGTTATGA CCATGGTTCA TGGGGACAAC ATGGGTTTAG TATTACCACC CCGTGTAGCA 
TGTGTTCAGG TGGTGATTAT TCCTTGTGGC ATTACCAATG CACTTTCTGA AGAAGACAAA 
GAAGCGCTGA TTGCAAAATG CAATGATTAT CGAAGGCGAT TACTCAGTGT TAACATCCGC 
GTTAGAGCTG ATTTACGAGA TAATTATTCT CCAGGTTGGA AATTCAATCA CTGGGAGCTC 
AAGGGAGTTC CCATTAGACT TGAAGTTGGG CCACGTGATA TGAAGAGCTG TCAGTTTGTA 
GCCGTCAGAC GAGATACTGG AGAAAAGCTG ACAGTTGCTG AAAATGAGGC AGAGACTAAA 
CTTCAAGCTA TTTTGGAAGA CATCCAGGTC ACCCTTTTCA CAAGGGCTTC TGAAGACCTT 
AAGACTCATA TGGTTGTGGC TAATACAATG GAAGACTTTC AGAAGATACT AGATTCTGGA 
AAGATTGTTC AGATTCCATT CTGTGGGGAA ATTGACTGTG AGGACTGGAT CAAAAAGACC 
ACTGCCAGGG ATCAAGATCT TGAACCTGGT GCTCCATCCA TGGGAGCTAA AAGCCTTTGC 
ATCCCCTTCA AACCACTCTG TGAACTGCAG CCTGGAGCCA AATGTGTCTG TGGCAAGAAC 
CCTGCCAAGT ACTACACCTT ATTTGGTCGC AGCTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EPRS ( NM_004446.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_004437.2 Homo sapiens glutamyl-prolyl-tRNA synthetase (EPRS), mRNA.

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CloneID	OHu04348
Clone ID Related Accession (Same CDS sequence)	NM_004446.3 , NM_004446.2
Accession Version	NM_004446.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4539bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-26
Organism	Homo sapiens(human)
Product	bifunctional glutamate/proline--tRNA ligase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC046156.1, BC034797.1, AY493416.1, N85252.1 and AA196283.1. On Apr 6, 2005 this sequence version replaced NM_004446.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.52922.1, SRR1660809.14153.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: full length.

ATGGCGACGC TCTCTCTGAC CGTGAATTCA GGAGACCCTC CGCTAGGAGC TTTGCTGGCA 
GTAGAACACG TGAAAGACGA TGTCAGCATT TCCGTTGAAG AAGGGAAAGA GAATATTCTT 
CATGTTTCTG AAAATGTGAT ATTCACAGAT GTGAATTCTA TACTTCGCTA CTTGGCTAGA 
GTTGCAACTA CAGCTGGGTT ATATGGCTCT AATCTGATGG AACATACTGA GATTGATCAC 
TGGTTGGAGT TCAGTGCTAC AAAATTATCT TCATGTGATT CCTTTACTTC TACAATTAAT 
GAACTCAATC ATTGCCTGTC TCTGAGAACA TACTTAGTTG GAAACTCCTT GAGTTTAGCA 
GATTTATGTG TTTGGGCCAC CCTAAAAGGA AATGCTGCCT GGCAAGAACA GTTGAAACAG 
AAGAAAGCTC CAGTTCATGT AAAACGTTGG TTTGGCTTTC TTGAAGCCCA GCAGGCCTTC 
CAGTCAGTAG GTACCAAGTG GGATGTTTCA ACAACCAAAG CTCGAGTGGC ACCTGAGAAA 
AAGCAAGATG TTGGGAAATT TGTTGAGCTT CCAGGTGCGG AGATGGGAAA GGTTACCGTC 
AGATTTCCTC CAGAGGCCAG TGGTTACTTA CACATTGGGC ATGCAAAAGC TGCTCTTCTG 
AACCAGCACT ACCAGGTTAA CTTTAAAGGG AAACTGATCA TGAGATTTGA TGACACAAAT 
CCTGAAAAAG AAAAGGAAGA TTTTGAGAAG GTTATCTTGG AAGATGTTGC AATGTTGCAT 
ATCAAACCAG ATCAATTTAC TTATACTTCG GATCATTTTG AAACTATAAT GAAGTATGCA 
GAGAAGCTAA TTCAAGAAGG GAAGGCTTAT GTGGATGATA CTCCTGCTGA ACAGATGAAA 
GCAGAACGTG AGCAGAGGAT AGACTCTAAA CATAGAAAAA ACCCTATTGA GAAGAATCTA 
CAAATGTGGG AAGAAATGAA AAAAGGGAGC CAGTTTGGTC AGTCCTGTTG TTTGCGAGCA 
AAAATTGACA TGAGTAGTAA CAATGGATGC ATGAGAGATC CAACCCTTTA TCGCTGCAAA 
ATTCAACCAC ATCCAAGAAC TGGAAATAAA TACAATGTTT ATCCAACATA TGATTTTGCC 
TGCCCCATAG TTGACAGCAT CGAAGGTGTT ACACATGCCC TGAGAACAAC AGAATACCAT 
GACAGAGATG AGCAGTTTTA CTGGATTATT GAAGCTTTAG GCATAAGAAA ACCATATATT 
TGGGAATATA GTCGGCTAAA TCTCAACAAC ACAGTGCTAT CCAAAAGAAA ACTCACATGG 
TTTGTCAATG AAGGACTAGT AGATGGATGG GATGACCCAA GATTTCCTAC GGTTCGTGGT 
GTACTGAGAA GAGGGATGAC AGTTGAAGGA CTGAAACAGT TTATTGCTGC TCAGGGCTCC 
TCACGTTCAG TCGTGAACAT GGAGTGGGAC AAAATCTGGG CGTTTAACAA AAAGGTTATT 
GACCCAGTGG CTCCACGATA TGTTGCATTA CTGAAGAAAG AAGTGATCCC AGTGAATGTA 
CCTGAAGCTC AGGAGGAGAT GAAAGAAGTA GCCAAACACC CAAAGAATCC TGAGGTTGGC 
TTGAAGCCTG TGTGGTATAG TCCCAAAGTT TTCATTGAAG GTGCTGATGC AGAGACTTTT 
TCGGAGGGTG AGATGGTTAC ATTTATAAAT TGGGGCAACC TCAACATTAC AAAAATACAC 
AAAAATGCAG ATGGAAAAAT CATATCTCTT GATGCAAAGT TGAATTTGGA AAACAAAGAC 
TACAAGAAAA CCACTAAGGT CACTTGGCTT GCAGAGACTA CACATGCTCT TCCTATTCCA 
GTAATCTGTG TCACTTATGA GCACTTGATC ACAAAGCCAG TGCTAGGAAA AGACGAGGAC 
TTTAAGCAGT ATGTCAACAA GAACAGTAAG CATGAAGAGC TAATGCTAGG GGATCCCTGC 
CTTAAGGATT TGAAAAAAGG AGATATTATA CAACTCCAGA GAAGAGGATT CTTCATATGT 
GATCAACCTT ATGAACCTGT TAGCCCATAT AGTTGCAAGG AAGCCCCGTG TGTTTTGATA 
TACATTCCTG ATGGGCACAC AAAGGAAATG CCAACATCAG GGTCAAAGGA AAAGACCAAA 
GTAGAAGCCA CAAAAAATGA GACCTCTGCT CCTTTTAAGG AAAGACCAAC ACCTTCTCTG 
AATAATAATT GTACTACATC TGAGGATTCC TTGGTCCTTT ACAATAGAGT GGCTGTTCAA 
GGAGATGTGG TTCGTGAATT AAAAGCCAAG AAAGCACCAA AGGAAGATGT AGATGCAGCT 
GTAAAACAGC TTTTGTCTTT GAAAGCTGAA TATAAGGAGA AAACTGGCCA GGAATATAAA 
CCTGGAAACC CTCCTGCTGA AATAGGACAG AATATTTCTT CTAATTCCTC AGCAAGTATT 
CTGGAAAGTA AATCTCTGTA TGATGAAGTT GCTGCACAAG GGGAGGTGGT TCGTAAGCTA 
AAAGCTGAAA AATCCCCTAA GGCTAAAATA AATGAAGCTG TAGAATGCTT ACTGTCCCTG 
AAGGCTCAGT ATAAAGAAAA AACTGGGAAG GAGTACATAC CTGGTCAGCC CCCATTATCT 
CAAAGTTCGG ATTCAAGCCC AACCAGAAAT TCTGAACCTG CTGGTTTAGA AACACCAGAA 
GCGAAAGTAC TTTTTGACAA AGTAGCTTCT CAAGGGGAAG TAGTTCGGAA ACTTAAAACT 
GAAAAAGCCC CTAAGGATCA AGTAGATATA GCTGTTCAAG AACTCCTTCA GCTAAAGGCA 
CAGTACAAGT CTTTGATAGG AGTAGAGTAT AAGCCTGTGT CGGCCACTGG AGCTGAGGAC 
AAAGATAAGA AGAAGAAAGA AAAAGAAAAT AAATCTGAAA AGCAGAATAA GCCTCAGAAA 
CAAAATGATG GCCAAAGGAA AGACCCTTCT AAAAACCAAG GAGGTGGGCT CTCATCAAGT 
GGAGCAGGAG AAGGGCAGGG GCCTAAGAAA CAGACCAGGT TGGGTCTTGA GGCAAAAAAA 
GAAGAAAATC TTGCTGATTG GTATTCTCAG GTCATCACAA AGTCAGAAAT GATTGAATAC 
CATGACATAA GTGGCTGTTA TATTCTTCGT CCCTGGGCCT ATGCCATTTG GGAAGCCATC 
AAGGACTTTT TTGATGCTGA GATCAAGAAA CTTGGTGTTG AAAACTGCTA CTTCCCCATG 
TTTGTGTCTC AAAGTGCATT AGAGAAAGAG AAGACTCATG TTGCTGACTT TGCCCCAGAG 
GTTGCTTGGG TTACAAGATC TGGCAAAACC GAGCTGGCAG AACCAATTGC CATTCGTCCT 
ACTAGTGAAA CAGTAATGTA TCCTGCATAT GCAAAATGGG TACAGTCACA CAGAGACCTG 
CCCATCAAGC TCAATCAGTG GTGCAATGTG GTGCGTTGGG AATTCAAGCA TCCTCAGCCT 
TTCCTACGTA CTCGTGAATT TCTTTGGCAG GAAGGGCACA GTGCTTTTGC TACCATGGAA 
GAGGCAGCGG AAGAGGTCTT GCAGATACTT GACTTATATG CTCAGGTATA TGAAGAACTC 
CTGGCAATTC CTGTTGTTAA AGGAAGAAAG ACGGAAAAGG AAAAATTTGC AGGAGGAGAC 
TATACAACTA CAATAGAAGC ATTTATATCT GCTAGTGGAA GAGCTATCCA GGGAGGAACA 
TCACATCATT TAGGGCAGAA TTTTTCCAAA ATGTTTGAAA TCGTTTTTGA AGATCCAAAG 
ATACCAGGAG AGAAGCAATT TGCCTATCAA AACTCCTGGG GCCTGACAAC TCGAACTATT 
GGTGTTATGA CCATGGTTCA TGGGGACAAC ATGGGTTTAG TATTACCACC CCGTGTAGCA 
TGTGTTCAGG TGGTGATTAT TCCTTGTGGC ATTACCAATG CACTTTCTGA AGAAGACAAA 
GAAGCGCTGA TTGCAAAATG CAATGATTAT CGAAGGCGAT TACTCAGTGT TAACATCCGC 
GTTAGAGCTG ATTTACGAGA TAATTATTCT CCAGGTTGGA AATTCAATCA CTGGGAGCTC 
AAGGGAGTTC CCATTAGACT TGAAGTTGGG CCACGTGATA TGAAGAGCTG TCAGTTTGTA 
GCCGTCAGAC GAGATACTGG AGAAAAGCTG ACAGTTGCTG AAAATGAGGC AGAGACTAAA 
CTTCAAGCTA TTTTGGAAGA CATCCAGGTC ACCCTTTTCA CAAGGGCTTC TGAAGACCTT 
AAGACTCATA TGGTTGTGGC TAATACAATG GAAGACTTTC AGAAGATACT AGATTCTGGA 
AAGATTGTTC AGATTCCATT CTGTGGGGAA ATTGACTGTG AGGACTGGAT CAAAAAGACC 
ACTGCCAGGG ATCAAGATCT TGAACCTGGT GCTCCATCCA TGGGAGCTAA AAGCCTTTGC 
ATCCCCTTCA AACCACTCTG TGAACTGCAG CCTGGAGCCA AATGTGTCTG TGGCAAGAAC 
CCTGCCAAGT ACTACACCTT ATTTGGTCGC AGCTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004437.2
CDS	271..4809
Translation	MATLSLTVNSGDPPLGALLAVEHVKDDVSISVEEGKENILHVSENVIFTDVNSILRYLARVATTAGLYGSNLMEHTEIDHWLEFSATKLSSCDSFTSTINELNHCLSLRTYLVGNSLSLADLCVWATLKGNAAWQEQLKQKKAPVHVKRWFGFLEAQQAFQSVGTKWDVSTTKARVAPEKKQDVGKFVELPGAEMGKVTVRFPPEASGYLHIGHAKAALLNQHYQVNFKGKLIMRFDDTNPEKEKEDFEKVILEDVAMLHIKPDQFTYTSDHFETIMKYAEKLIQEGKAYVDDTPAEQMKAEREQRIDSKHRKNPIEKNLQMWEEMKKGSQFGQSCCLRAKIDMSSNNGCMRDPTLYRCKIQPHPRTGNKYNVYPTYDFACPIVDSIEGVTHALRTTEYHDRDEQFYWIIEALGIRKPYIWEYSRLNLNNTVLSKRKLTWFVNEGLVDGWDDPRFPTVRGVLRRGMTVEGLKQFIAAQGSSRSVVNMEWDKIWAFNKKVIDPVAPRYVALLKKEVIPVNVPEAQEEMKEVAKHPKNPEVGLKPVWYSPKVFIEGADAETFSEGEMVTFINWGNLNITKIHKNADGKIISLDAKLNLENKDYKKTTKVTWLAETTHALPIPVICVTYEHLITKPVLGKDEDFKQYVNKNSKHEELMLGDPCLKDLKKGDIIQLQRRGFFICDQPYEPVSPYSCKEAPCVLIYIPDGHTKEMPTSGSKEKTKVEATKNETSAPFKERPTPSLNNNCTTSEDSLVLYNRVAVQGDVVRELKAKKAPKEDVDAAVKQLLSLKAEYKEKTGQEYKPGNPPAEIGQNISSNSSASILESKSLYDEVAAQGEVVRKLKAEKSPKAKINEAVECLLSLKAQYKEKTGKEYIPGQPPLSQSSDSSPTRNSEPAGLETPEAKVLFDKVASQGEVVRKLKTEKAPKDQVDIAVQELLQLKAQYKSLIGVEYKPVSATGAEDKDKKKKEKENKSEKQNKPQKQNDGQRKDPSKNQGGGLSSSGAGEGQGPKKQTRLGLEAKKEENLADWYSQVITKSEMIEYHDISGCYILRPWAYAIWEAIKDFFDAEIKKLGVENCYFPMFVSQSALEKEKTHVADFAPEVAWVTRSGKTELAEPIAIRPTSETVMYPAYAKWVQSHRDLPIKLNQWCNVVRWEFKHPQPFLRTREFLWQEGHSAFATMEEAAEEVLQILDLYAQVYEELLAIPVVKGRKTEKEKFAGGDYTTTIEAFISASGRAIQGGTSHHLGQNFSKMFEIVFEDPKIPGEKQFAYQNSWGLTTRTIGVMTMVHGDNMGLVLPPRVACVQVVIIPCGITNALSEEDKEALIAKCNDYRRRLLSVNIRVRADLRDNYSPGWKFNHWELKGVPIRLEVGPRDMKSCQFVAVRRDTGEKLTVAENEAETKLQAILEDIQVTLFTRASEDLKTHMVVANTMEDFQKILDSGKIVQIPFCGEIDCEDWIKKTTARDQDLEPGAPSMGAKSLCIPFKPLCELQPGAKCVCGKNPAKYYTLFGRSY

Target ORF information:

RefSeq Version	NM_004446.2
Organism	Homo sapiens(human)
Definition	Homo sapiens glutamyl-prolyl-tRNA synthetase (EPRS), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004446.2

ORF Insert Sequence:

ATGGCGACGC TCTCTCTGAC CGTGAATTCA GGAGACCCTC CGCTAGGAGC TTTGCTGGCA 
GTAGAACACG TGAAAGACGA TGTCAGCATT TCCGTTGAAG AAGGGAAAGA GAATATTCTT 
CATGTTTCTG AAAATGTGAT ATTCACAGAT GTGAATTCTA TACTTCGCTA CTTGGCTAGA 
GTTGCAACTA CAGCTGGGTT ATATGGCTCT AATCTGATGG AACATACTGA GATTGATCAC 
TGGTTGGAGT TCAGTGCTAC AAAATTATCT TCATGTGATT CCTTTACTTC TACAATTAAT 
GAACTCAATC ATTGCCTGTC TCTGAGAACA TACTTAGTTG GAAACTCCTT GAGTTTAGCA 
GATTTATGTG TTTGGGCCAC CCTAAAAGGA AATGCTGCCT GGCAAGAACA GTTGAAACAG 
AAGAAAGCTC CAGTTCATGT AAAACGTTGG TTTGGCTTTC TTGAAGCCCA GCAGGCCTTC 
CAGTCAGTAG GTACCAAGTG GGATGTTTCA ACAACCAAAG CTCGAGTGGC ACCTGAGAAA 
AAGCAAGATG TTGGGAAATT TGTTGAGCTT CCAGGTGCGG AGATGGGAAA GGTTACCGTC 
AGATTTCCTC CAGAGGCCAG TGGTTACTTA CACATTGGGC ATGCAAAAGC TGCTCTTCTG 
AACCAGCACT ACCAGGTTAA CTTTAAAGGG AAACTGATCA TGAGATTTGA TGACACAAAT 
CCTGAAAAAG AAAAGGAAGA TTTTGAGAAG GTTATCTTGG AAGATGTTGC AATGTTGCAT 
ATCAAACCAG ATCAATTTAC TTATACTTCG GATCATTTTG AAACTATAAT GAAGTATGCA 
GAGAAGCTAA TTCAAGAAGG GAAGGCTTAT GTGGATGATA CTCCTGCTGA ACAGATGAAA 
GCAGAACGTG AGCAGAGGAT AGACTCTAAA CATAGAAAAA ACCCTATTGA GAAGAATCTA 
CAAATGTGGG AAGAAATGAA AAAAGGGAGC CAGTTTGGTC AGTCCTGTTG TTTGCGAGCA 
AAAATTGACA TGAGTAGTAA CAATGGATGC ATGAGAGATC CAACCCTTTA TCGCTGCAAA 
ATTCAACCAC ATCCAAGAAC TGGAAATAAA TACAATGTTT ATCCAACATA TGATTTTGCC 
TGCCCCATAG TTGACAGCAT CGAAGGTGTT ACACATGCCC TGAGAACAAC AGAATACCAT 
GACAGAGATG AGCAGTTTTA CTGGATTATT GAAGCTTTAG GCATAAGAAA ACCATATATT 
TGGGAATATA GTCGGCTAAA TCTCAACAAC ACAGTGCTAT CCAAAAGAAA ACTCACATGG 
TTTGTCAATG AAGGACTAGT AGATGGATGG GATGACCCAA GATTTCCTAC GGTTCGTGGT 
GTACTGAGAA GAGGGATGAC AGTTGAAGGA CTGAAACAGT TTATTGCTGC TCAGGGCTCC 
TCACGTTCAG TCGTGAACAT GGAGTGGGAC AAAATCTGGG CGTTTAACAA AAAGGTTATT 
GACCCAGTGG CTCCACGATA TGTTGCATTA CTGAAGAAAG AAGTGATCCC AGTGAATGTA 
CCTGAAGCTC AGGAGGAGAT GAAAGAAGTA GCCAAACACC CAAAGAATCC TGAGGTTGGC 
TTGAAGCCTG TGTGGTATAG TCCCAAAGTT TTCATTGAAG GTGCTGATGC AGAGACTTTT 
TCGGAGGGTG AGATGGTTAC ATTTATAAAT TGGGGCAACC TCAACATTAC AAAAATACAC 
AAAAATGCAG ATGGAAAAAT CATATCTCTT GATGCAAAGT TGAATTTGGA AAACAAAGAC 
TACAAGAAAA CCACTAAGGT CACTTGGCTT GCAGAGACTA CACATGCTCT TCCTATTCCA 
GTAATCTGTG TCACTTATGA GCACTTGATC ACAAAGCCAG TGCTAGGAAA AGACGAGGAC 
TTTAAGCAGT ATGTCAACAA GAACAGTAAG CATGAAGAGC TAATGCTAGG GGATCCCTGC 
CTTAAGGATT TGAAAAAAGG AGATATTATA CAACTCCAGA GAAGAGGATT CTTCATATGT 
GATCAACCTT ATGAACCTGT TAGCCCATAT AGTTGCAAGG AAGCCCCGTG TGTTTTGATA 
TACATTCCTG ATGGGCACAC AAAGGAAATG CCAACATCAG GGTCAAAGGA AAAGACCAAA 
GTAGAAGCCA CAAAAAATGA GACCTCTGCT CCTTTTAAGG AAAGACCAAC ACCTTCTCTG 
AATAATAATT GTACTACATC TGAGGATTCC TTGGTCCTTT ACAATAGAGT GGCTGTTCAA 
GGAGATGTGG TTCGTGAATT AAAAGCCAAG AAAGCACCAA AGGAAGATGT AGATGCAGCT 
GTAAAACAGC TTTTGTCTTT GAAAGCTGAA TATAAGGAGA AAACTGGCCA GGAATATAAA 
CCTGGAAACC CTCCTGCTGA AATAGGACAG AATATTTCTT CTAATTCCTC AGCAAGTATT 
CTGGAAAGTA AATCTCTGTA TGATGAAGTT GCTGCACAAG GGGAGGTGGT TCGTAAGCTA 
AAAGCTGAAA AATCCCCTAA GGCTAAAATA AATGAAGCTG TAGAATGCTT ACTGTCCCTG 
AAGGCTCAGT ATAAAGAAAA AACTGGGAAG GAGTACATAC CTGGTCAGCC CCCATTATCT 
CAAAGTTCGG ATTCAAGCCC AACCAGAAAT TCTGAACCTG CTGGTTTAGA AACACCAGAA 
GCGAAAGTAC TTTTTGACAA AGTAGCTTCT CAAGGGGAAG TAGTTCGGAA ACTTAAAACT 
GAAAAAGCCC CTAAGGATCA AGTAGATATA GCTGTTCAAG AACTCCTTCA GCTAAAGGCA 
CAGTACAAGT CTTTGATAGG AGTAGAGTAT AAGCCTGTGT CGGCCACTGG AGCTGAGGAC 
AAAGATAAGA AGAAGAAAGA AAAAGAAAAT AAATCTGAAA AGCAGAATAA GCCTCAGAAA 
CAAAATGATG GCCAAAGGAA AGACCCTTCT AAAAACCAAG GAGGTGGGCT CTCATCAAGT 
GGAGCAGGAG AAGGGCAGGG GCCTAAGAAA CAGACCAGGT TGGGTCTTGA GGCAAAAAAA 
GAAGAAAATC TTGCTGATTG GTATTCTCAG GTCATCACAA AGTCAGAAAT GATTGAATAC 
CATGACATAA GTGGCTGTTA TATTCTTCGT CCCTGGGCCT ATGCCATTTG GGAAGCCATC 
AAGGACTTTT TTGATGCTGA GATCAAGAAA CTTGGTGTTG AAAACTGCTA CTTCCCCATG 
TTTGTGTCTC AAAGTGCATT AGAGAAAGAG AAGACTCATG TTGCTGACTT TGCCCCAGAG 
GTTGCTTGGG TTACAAGATC TGGCAAAACC GAGCTGGCAG AACCAATTGC CATTCGTCCT 
ACTAGTGAAA CAGTAATGTA TCCTGCATAT GCAAAATGGG TACAGTCACA CAGAGACCTG 
CCCATCAAGC TCAATCAGTG GTGCAATGTG GTGCGTTGGG AATTCAAGCA TCCTCAGCCT 
TTCCTACGTA CTCGTGAATT TCTTTGGCAG GAAGGGCACA GTGCTTTTGC TACCATGGAA 
GAGGCAGCGG AAGAGGTCTT GCAGATACTT GACTTATATG CTCAGGTATA TGAAGAACTC 
CTGGCAATTC CTGTTGTTAA AGGAAGAAAG ACGGAAAAGG AAAAATTTGC AGGAGGAGAC 
TATACAACTA CAATAGAAGC ATTTATATCT GCTAGTGGAA GAGCTATCCA GGGAGGAACA 
TCACATCATT TAGGGCAGAA TTTTTCCAAA ATGTTTGAAA TCGTTTTTGA AGATCCAAAG 
ATACCAGGAG AGAAGCAATT TGCCTATCAA AACTCCTGGG GCCTGACAAC TCGAACTATT 
GGTGTTATGA CCATGGTTCA TGGGGACAAC ATGGGTTTAG TATTACCACC CCGTGTAGCA 
TGTGTTCAGG TGGTGATTAT TCCTTGTGGC ATTACCAATG CACTTTCTGA AGAAGACAAA 
GAAGCGCTGA TTGCAAAATG CAATGATTAT CGAAGGCGAT TACTCAGTGT TAACATCCGC 
GTTAGAGCTG ATTTACGAGA TAATTATTCT CCAGGTTGGA AATTCAATCA CTGGGAGCTC 
AAGGGAGTTC CCATTAGACT TGAAGTTGGG CCACGTGATA TGAAGAGCTG TCAGTTTGTA 
GCCGTCAGAC GAGATACTGG AGAAAAGCTG ACAGTTGCTG AAAATGAGGC AGAGACTAAA 
CTTCAAGCTA TTTTGGAAGA CATCCAGGTC ACCCTTTTCA CAAGGGCTTC TGAAGACCTT 
AAGACTCATA TGGTTGTGGC TAATACAATG GAAGACTTTC AGAAGATACT AGATTCTGGA 
AAGATTGTTC AGATTCCATT CTGTGGGGAA ATTGACTGTG AGGACTGGAT CAAAAAGACC 
ACTGCCAGGG ATCAAGATCT TGAACCTGGT GCTCCATCCA TGGGAGCTAA AAGCCTTTGC 
ATCCCCTTCA AACCACTCTG TGAACTGCAG CCTGGAGCCA AATGTGTCTG TGGCAAGAAC 
CCTGCCAAGT ACTACACCTT ATTTGGTCGC AGCTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EPRS ( XM_017000614.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016856103.1 Homo sapiens glutamyl-prolyl-tRNA synthetase 1 (EPRS1), transcript variant X1, mRNA.

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CloneID	OHu83326
Clone ID Related Accession (Same CDS sequence)	XM_017000614.2 , XM_017000614.1
Accession Version	XM_017000614.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2961bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	bifunctional glutamate/proline--tRNA ligase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017000614.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGAAG TAGCCAAACA CCCAAAGAAT CCTGAGGTTG GCTTGAAGCC TGTGTGGTAT 
AGTCCCAAAG TTTTCATTGA AGGTGCTGAT GCAGAGACTT TTTCGGAGGG TGAGATGGTT 
ACATTTATAA ATTGGGGCAA CCTCAACATT ACAAAAATAC ACAAAAATGC AGATGGAAAA 
ATCATATCTC TTGATGCAAA GTTGAATTTG GAAAACAAAG ACTACAAGAA AACCACTAAG 
GTCACTTGGC TTGCAGAGAC TACACATGCT CTTCCTATTC CAGTAATCTG TGTCACTTAT 
GAGCACTTGA TCACAAAGCC AGTGCTAGGA AAAGACGAGG ACTTTAAGCA GTATGTCAAC 
AAGAACAGTA AGCATGAAGA GCTAATGCTA GGGGATCCCT GCCTTAAGGA TTTGAAAAAA 
GGAGATATTA TACAACTCCA GAGAAGAGGA TTCTTCATAT GTGATCAACC TTATGAACCT 
GTTAGCCCAT ATAGTTGCAA GGAAGCCCCG TGTGTTTTGA TATACATTCC TGATGGGCAC 
ACAAAGGAAA TGCCAACATC AGGGTCAAAG GAAAAGACCA AAGTAGAAGC CACAAAAAAT 
GAGACCTCTG CTCCTTTTAA GGAAAGACCA ACACCTTCTC TGAATAATAA TTGTACTACA 
TCTGAGGATT CCTTGGTCCT TTACAATAGA GTGGCTGTTC AAGGAGATGT GGTTCGTGAA 
TTAAAAGCCA AGAAAGCACC AAAGGAAGAT GTAGATGCAG CTGTAAAACA GCTTTTGTCT 
TTGAAAGCTG AATATAAGGA GAAAACTGGC CAGGAATATA AACCTGGAAA CCCTCCTGCT 
GAAATAGGAC AGAATATTTC TTCTAATTCC TCAGCAAGTA TTCTGGAAAG TAAATCTCTG 
TATGATGAAG TTGCTGCACA AGGGGAGGTG GTTCGTAAGC TAAAAGCTGA AAAATCCCCT 
AAGGCTAAAA TAAATGAAGC TGTAGAATGC TTACTGTCCC TGAAGGCTCA GTATAAAGAA 
AAAACTGGGA AGGAGTACAT ACCTGGTCAG CCCCCATTAT CTCAAAGTTC GGATTCAAGC 
CCAACCAGAA ATTCTGAACC TGCTGGTTTA GAAACACCAG AAGCGAAAGT ACTTTTTGAC 
AAAGTAGCTT CTCAAGGGGA AGTAGTTCGG AAACTTAAAA CTGAAAAAGC CCCTAAGGAT 
CAAGTAGATA TAGCTGTTCA AGAACTCCTT CAGCTAAAGG CACAGTACAA GTCTTTGATA 
GGAGTAGAGT ATAAGCCTGT GTCGGCCACT GGAGCTGAGG ACAAAGATAA GAAGAAGAAA 
GAAAAAGAAA ATAAATCTGA AAAGCAGAAT AAGCCTCAGA AACAAAATGA TGGCCAAAGG 
AAAGACCCTT CTAAAAACCA AGGAGGTGGG CTCTCATCAA GTGGAGCAGG AGAAGGGCAG 
GGGCCTAAGA AACAGACCAG GTTGGGTCTT GAGGCAAAAA AAGAAGAAAA TCTTGCTGAT 
TGGTATTCTC AGGTCATCAC AAAGTCAGAA ATGATTGAAT ACCATGACAT AAGTGGCTGT 
TATATTCTTC GTCCCTGGGC CTATGCCATT TGGGAAGCCA TCAAGGACTT TTTTGATGCT 
GAGATCAAGA AACTTGGTGT TGAAAACTGC TACTTCCCCA TGTTTGTGTC TCAAAGTGCA 
TTAGAGAAAG AGAAGACTCA TGTTGCTGAC TTTGCCCCAG AGGTTGCTTG GGTTACAAGA 
TCTGGCAAAA CCGAGCTGGC AGAACCAATT GCCATTCGTC CTACTAGTGA AACAGTAATG 
TATCCTGCAT ATGCAAAATG GGTACAGTCA CACAGAGACC TGCCCATCAA GCTCAATCAG 
TGGTGCAATG TGGTGCGTTG GGAATTCAAG CATCCTCAGC CTTTCCTACG TACTCGTGAA 
TTTCTTTGGC AGGAAGGGCA CAGTGCTTTT GCTACCATGG AAGAGGCAGC GGAAGAGGTC 
TTGCAGATAC TTGACTTATA TGCTCAGGTA TATGAAGAAC TCCTGGCAAT TCCTGTTGTT 
AAAGGAAGAA AGACGGAAAA GGAAAAATTT GCAGGAGGAG ACTATACAAC TACAATAGAA 
GCATTTATAT CTGCTAGTGG AAGAGCTATC CAGGGAGGAA CATCACATCA TTTAGGGCAG 
AATTTTTCCA AAATGTTTGA AATCGTTTTT GAAGATCCAA AGATACCAGG AGAGAAGCAA 
TTTGCCTATC AAAACTCCTG GGGCCTGACA ACTCGAACTA TTGGTGTTAT GACCATGGTT 
CATGGGGACA ACATGGGTTT AGTATTACCA CCCCGTGTAG CATGTGTTCA GGTGGTGATT 
ATTCCTTGTG GCATTACCAA TGCACTTTCT GAAGAAGACA AAGAAGCGCT GATTGCAAAA 
TGCAATGATT ATCGAAGGCG ATTACTCAGT GTTAACATCC GCGTTAGAGC TGATTTACGA 
GATAATTATT CTCCAGGTTG GAAATTCAAT CACTGGGAGC TCAAGGGAGT TCCCATTAGA 
CTTGAAGTTG GGCCACGTGA TATGAAGAGC TGTCAGTTTG TAGCCGTCAG ACGAGATACT 
GGAGAAAAGC TGACAGTTGC TGAAAATGAG GCAGAGACTA AACTTCAAGC TATTTTGGAA 
GACATCCAGG TCACCCTTTT CACAAGGGCT TCTGAAGACC TTAAGACTCA TATGGTTGTG 
GCTAATACAA TGGAAGACTT TCAGAAGATA CTAGATTCTG GAAAGATTGT TCAGATTCCA 
TTCTGTGGGG AAATTGACTG TGAGGACTGG ATCAAAAAGA CCACTGCCAG GGATCAAGAT 
CTTGAACCTG GTGCTCCATC CATGGGAGCT AAAAGCCTTT GCATCCCCTT CAAACCACTC 
TGTGAACTGC AGCCTGGAGC CAAATGTGTC TGTGGCAAGA ACCCTGCCAA GTACTACACC 
TTATTTGGTC GCAGCTACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016856103.1
CDS	104..3064
Translation	MKEVAKHPKNPEVGLKPVWYSPKVFIEGADAETFSEGEMVTFINWGNLNITKIHKNADGKIISLDAKLNLENKDYKKTTKVTWLAETTHALPIPVICVTYEHLITKPVLGKDEDFKQYVNKNSKHEELMLGDPCLKDLKKGDIIQLQRRGFFICDQPYEPVSPYSCKEAPCVLIYIPDGHTKEMPTSGSKEKTKVEATKNETSAPFKERPTPSLNNNCTTSEDSLVLYNRVAVQGDVVRELKAKKAPKEDVDAAVKQLLSLKAEYKEKTGQEYKPGNPPAEIGQNISSNSSASILESKSLYDEVAAQGEVVRKLKAEKSPKAKINEAVECLLSLKAQYKEKTGKEYIPGQPPLSQSSDSSPTRNSEPAGLETPEAKVLFDKVASQGEVVRKLKTEKAPKDQVDIAVQELLQLKAQYKSLIGVEYKPVSATGAEDKDKKKKEKENKSEKQNKPQKQNDGQRKDPSKNQGGGLSSSGAGEGQGPKKQTRLGLEAKKEENLADWYSQVITKSEMIEYHDISGCYILRPWAYAIWEAIKDFFDAEIKKLGVENCYFPMFVSQSALEKEKTHVADFAPEVAWVTRSGKTELAEPIAIRPTSETVMYPAYAKWVQSHRDLPIKLNQWCNVVRWEFKHPQPFLRTREFLWQEGHSAFATMEEAAEEVLQILDLYAQVYEELLAIPVVKGRKTEKEKFAGGDYTTTIEAFISASGRAIQGGTSHHLGQNFSKMFEIVFEDPKIPGEKQFAYQNSWGLTTRTIGVMTMVHGDNMGLVLPPRVACVQVVIIPCGITNALSEEDKEALIAKCNDYRRRLLSVNIRVRADLRDNYSPGWKFNHWELKGVPIRLEVGPRDMKSCQFVAVRRDTGEKLTVAENEAETKLQAILEDIQVTLFTRASEDLKTHMVVANTMEDFQKILDSGKIVQIPFCGEIDCEDWIKKTTARDQDLEPGAPSMGAKSLCIPFKPLCELQPGAKCVCGKNPAKYYTLFGRSY

Target ORF information:

RefSeq Version	XM_017000614.2
Organism	Homo sapiens(human)
Definition	Homo sapiens glutamyl-prolyl-tRNA synthetase 1 (EPRS1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017000614.2

ORF Insert Sequence:

ATGAAAGAAG TAGCCAAACA CCCAAAGAAT CCTGAGGTTG GCTTGAAGCC TGTGTGGTAT 
AGTCCCAAAG TTTTCATTGA AGGTGCTGAT GCAGAGACTT TTTCGGAGGG TGAGATGGTT 
ACATTTATAA ATTGGGGCAA CCTCAACATT ACAAAAATAC ACAAAAATGC AGATGGAAAA 
ATCATATCTC TTGATGCAAA GTTGAATTTG GAAAACAAAG ACTACAAGAA AACCACTAAG 
GTCACTTGGC TTGCAGAGAC TACACATGCT CTTCCTATTC CAGTAATCTG TGTCACTTAT 
GAGCACTTGA TCACAAAGCC AGTGCTAGGA AAAGACGAGG ACTTTAAGCA GTATGTCAAC 
AAGAACAGTA AGCATGAAGA GCTAATGCTA GGGGATCCCT GCCTTAAGGA TTTGAAAAAA 
GGAGATATTA TACAACTCCA GAGAAGAGGA TTCTTCATAT GTGATCAACC TTATGAACCT 
GTTAGCCCAT ATAGTTGCAA GGAAGCCCCG TGTGTTTTGA TATACATTCC TGATGGGCAC 
ACAAAGGAAA TGCCAACATC AGGGTCAAAG GAAAAGACCA AAGTAGAAGC CACAAAAAAT 
GAGACCTCTG CTCCTTTTAA GGAAAGACCA ACACCTTCTC TGAATAATAA TTGTACTACA 
TCTGAGGATT CCTTGGTCCT TTACAATAGA GTGGCTGTTC AAGGAGATGT GGTTCGTGAA 
TTAAAAGCCA AGAAAGCACC AAAGGAAGAT GTAGATGCAG CTGTAAAACA GCTTTTGTCT 
TTGAAAGCTG AATATAAGGA GAAAACTGGC CAGGAATATA AACCTGGAAA CCCTCCTGCT 
GAAATAGGAC AGAATATTTC TTCTAATTCC TCAGCAAGTA TTCTGGAAAG TAAATCTCTG 
TATGATGAAG TTGCTGCACA AGGGGAGGTG GTTCGTAAGC TAAAAGCTGA AAAATCCCCT 
AAGGCTAAAA TAAATGAAGC TGTAGAATGC TTACTGTCCC TGAAGGCTCA GTATAAAGAA 
AAAACTGGGA AGGAGTACAT ACCTGGTCAG CCCCCATTAT CTCAAAGTTC GGATTCAAGC 
CCAACCAGAA ATTCTGAACC TGCTGGTTTA GAAACACCAG AAGCGAAAGT ACTTTTTGAC 
AAAGTAGCTT CTCAAGGGGA AGTAGTTCGG AAACTTAAAA CTGAAAAAGC CCCTAAGGAT 
CAAGTAGATA TAGCTGTTCA AGAACTCCTT CAGCTAAAGG CACAGTACAA GTCTTTGATA 
GGAGTAGAGT ATAAGCCTGT GTCGGCCACT GGAGCTGAGG ACAAAGATAA GAAGAAGAAA 
GAAAAAGAAA ATAAATCTGA AAAGCAGAAT AAGCCTCAGA AACAAAATGA TGGCCAAAGG 
AAAGACCCTT CTAAAAACCA AGGAGGTGGG CTCTCATCAA GTGGAGCAGG AGAAGGGCAG 
GGGCCTAAGA AACAGACCAG GTTGGGTCTT GAGGCAAAAA AAGAAGAAAA TCTTGCTGAT 
TGGTATTCTC AGGTCATCAC AAAGTCAGAA ATGATTGAAT ACCATGACAT AAGTGGCTGT 
TATATTCTTC GTCCCTGGGC CTATGCCATT TGGGAAGCCA TCAAGGACTT TTTTGATGCT 
GAGATCAAGA AACTTGGTGT TGAAAACTGC TACTTCCCCA TGTTTGTGTC TCAAAGTGCA 
TTAGAGAAAG AGAAGACTCA TGTTGCTGAC TTTGCCCCAG AGGTTGCTTG GGTTACAAGA 
TCTGGCAAAA CCGAGCTGGC AGAACCAATT GCCATTCGTC CTACTAGTGA AACAGTAATG 
TATCCTGCAT ATGCAAAATG GGTACAGTCA CACAGAGACC TGCCCATCAA GCTCAATCAG 
TGGTGCAATG TGGTGCGTTG GGAATTCAAG CATCCTCAGC CTTTCCTACG TACTCGTGAA 
TTTCTTTGGC AGGAAGGGCA CAGTGCTTTT GCTACCATGG AAGAGGCAGC GGAAGAGGTC 
TTGCAGATAC TTGACTTATA TGCTCAGGTA TATGAAGAAC TCCTGGCAAT TCCTGTTGTT 
AAAGGAAGAA AGACGGAAAA GGAAAAATTT GCAGGAGGAG ACTATACAAC TACAATAGAA 
GCATTTATAT CTGCTAGTGG AAGAGCTATC CAGGGAGGAA CATCACATCA TTTAGGGCAG 
AATTTTTCCA AAATGTTTGA AATCGTTTTT GAAGATCCAA AGATACCAGG AGAGAAGCAA 
TTTGCCTATC AAAACTCCTG GGGCCTGACA ACTCGAACTA TTGGTGTTAT GACCATGGTT 
CATGGGGACA ACATGGGTTT AGTATTACCA CCCCGTGTAG CATGTGTTCA GGTGGTGATT 
ATTCCTTGTG GCATTACCAA TGCACTTTCT GAAGAAGACA AAGAAGCGCT GATTGCAAAA 
TGCAATGATT ATCGAAGGCG ATTACTCAGT GTTAACATCC GCGTTAGAGC TGATTTACGA 
GATAATTATT CTCCAGGTTG GAAATTCAAT CACTGGGAGC TCAAGGGAGT TCCCATTAGA 
CTTGAAGTTG GGCCACGTGA TATGAAGAGC TGTCAGTTTG TAGCCGTCAG ACGAGATACT 
GGAGAAAAGC TGACAGTTGC TGAAAATGAG GCAGAGACTA AACTTCAAGC TATTTTGGAA 
GACATCCAGG TCACCCTTTT CACAAGGGCT TCTGAAGACC TTAAGACTCA TATGGTTGTG 
GCTAATACAA TGGAAGACTT TCAGAAGATA CTAGATTCTG GAAAGATTGT TCAGATTCCA 
TTCTGTGGGG AAATTGACTG TGAGGACTGG ATCAAAAAGA CCACTGCCAG GGATCAAGAT 
CTTGAACCTG GTGCTCCATC CATGGGAGCT AAAAGCCTTT GCATCCCCTT CAAACCACTC 
TGTGAACTGC AGCCTGGAGC CAAATGTGTC TGTGGCAAGA ACCCTGCCAA GTACTACACC 
TTATTTGGTC GCAGCTACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EPRS ( XM_017000614.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016856103.1 Homo sapiens glutamyl-prolyl-tRNA synthetase (EPRS), transcript variant X1, mRNA.

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CloneID	OHu83326
Clone ID Related Accession (Same CDS sequence)	XM_017000614.2 , XM_017000614.1
Accession Version	XM_017000614.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2961bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	bifunctional glutamate/proline--tRNA ligase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGAAG TAGCCAAACA CCCAAAGAAT CCTGAGGTTG GCTTGAAGCC TGTGTGGTAT 
AGTCCCAAAG TTTTCATTGA AGGTGCTGAT GCAGAGACTT TTTCGGAGGG TGAGATGGTT 
ACATTTATAA ATTGGGGCAA CCTCAACATT ACAAAAATAC ACAAAAATGC AGATGGAAAA 
ATCATATCTC TTGATGCAAA GTTGAATTTG GAAAACAAAG ACTACAAGAA AACCACTAAG 
GTCACTTGGC TTGCAGAGAC TACACATGCT CTTCCTATTC CAGTAATCTG TGTCACTTAT 
GAGCACTTGA TCACAAAGCC AGTGCTAGGA AAAGACGAGG ACTTTAAGCA GTATGTCAAC 
AAGAACAGTA AGCATGAAGA GCTAATGCTA GGGGATCCCT GCCTTAAGGA TTTGAAAAAA 
GGAGATATTA TACAACTCCA GAGAAGAGGA TTCTTCATAT GTGATCAACC TTATGAACCT 
GTTAGCCCAT ATAGTTGCAA GGAAGCCCCG TGTGTTTTGA TATACATTCC TGATGGGCAC 
ACAAAGGAAA TGCCAACATC AGGGTCAAAG GAAAAGACCA AAGTAGAAGC CACAAAAAAT 
GAGACCTCTG CTCCTTTTAA GGAAAGACCA ACACCTTCTC TGAATAATAA TTGTACTACA 
TCTGAGGATT CCTTGGTCCT TTACAATAGA GTGGCTGTTC AAGGAGATGT GGTTCGTGAA 
TTAAAAGCCA AGAAAGCACC AAAGGAAGAT GTAGATGCAG CTGTAAAACA GCTTTTGTCT 
TTGAAAGCTG AATATAAGGA GAAAACTGGC CAGGAATATA AACCTGGAAA CCCTCCTGCT 
GAAATAGGAC AGAATATTTC TTCTAATTCC TCAGCAAGTA TTCTGGAAAG TAAATCTCTG 
TATGATGAAG TTGCTGCACA AGGGGAGGTG GTTCGTAAGC TAAAAGCTGA AAAATCCCCT 
AAGGCTAAAA TAAATGAAGC TGTAGAATGC TTACTGTCCC TGAAGGCTCA GTATAAAGAA 
AAAACTGGGA AGGAGTACAT ACCTGGTCAG CCCCCATTAT CTCAAAGTTC GGATTCAAGC 
CCAACCAGAA ATTCTGAACC TGCTGGTTTA GAAACACCAG AAGCGAAAGT ACTTTTTGAC 
AAAGTAGCTT CTCAAGGGGA AGTAGTTCGG AAACTTAAAA CTGAAAAAGC CCCTAAGGAT 
CAAGTAGATA TAGCTGTTCA AGAACTCCTT CAGCTAAAGG CACAGTACAA GTCTTTGATA 
GGAGTAGAGT ATAAGCCTGT GTCGGCCACT GGAGCTGAGG ACAAAGATAA GAAGAAGAAA 
GAAAAAGAAA ATAAATCTGA AAAGCAGAAT AAGCCTCAGA AACAAAATGA TGGCCAAAGG 
AAAGACCCTT CTAAAAACCA AGGAGGTGGG CTCTCATCAA GTGGAGCAGG AGAAGGGCAG 
GGGCCTAAGA AACAGACCAG GTTGGGTCTT GAGGCAAAAA AAGAAGAAAA TCTTGCTGAT 
TGGTATTCTC AGGTCATCAC AAAGTCAGAA ATGATTGAAT ACCATGACAT AAGTGGCTGT 
TATATTCTTC GTCCCTGGGC CTATGCCATT TGGGAAGCCA TCAAGGACTT TTTTGATGCT 
GAGATCAAGA AACTTGGTGT TGAAAACTGC TACTTCCCCA TGTTTGTGTC TCAAAGTGCA 
TTAGAGAAAG AGAAGACTCA TGTTGCTGAC TTTGCCCCAG AGGTTGCTTG GGTTACAAGA 
TCTGGCAAAA CCGAGCTGGC AGAACCAATT GCCATTCGTC CTACTAGTGA AACAGTAATG 
TATCCTGCAT ATGCAAAATG GGTACAGTCA CACAGAGACC TGCCCATCAA GCTCAATCAG 
TGGTGCAATG TGGTGCGTTG GGAATTCAAG CATCCTCAGC CTTTCCTACG TACTCGTGAA 
TTTCTTTGGC AGGAAGGGCA CAGTGCTTTT GCTACCATGG AAGAGGCAGC GGAAGAGGTC 
TTGCAGATAC TTGACTTATA TGCTCAGGTA TATGAAGAAC TCCTGGCAAT TCCTGTTGTT 
AAAGGAAGAA AGACGGAAAA GGAAAAATTT GCAGGAGGAG ACTATACAAC TACAATAGAA 
GCATTTATAT CTGCTAGTGG AAGAGCTATC CAGGGAGGAA CATCACATCA TTTAGGGCAG 
AATTTTTCCA AAATGTTTGA AATCGTTTTT GAAGATCCAA AGATACCAGG AGAGAAGCAA 
TTTGCCTATC AAAACTCCTG GGGCCTGACA ACTCGAACTA TTGGTGTTAT GACCATGGTT 
CATGGGGACA ACATGGGTTT AGTATTACCA CCCCGTGTAG CATGTGTTCA GGTGGTGATT 
ATTCCTTGTG GCATTACCAA TGCACTTTCT GAAGAAGACA AAGAAGCGCT GATTGCAAAA 
TGCAATGATT ATCGAAGGCG ATTACTCAGT GTTAACATCC GCGTTAGAGC TGATTTACGA 
GATAATTATT CTCCAGGTTG GAAATTCAAT CACTGGGAGC TCAAGGGAGT TCCCATTAGA 
CTTGAAGTTG GGCCACGTGA TATGAAGAGC TGTCAGTTTG TAGCCGTCAG ACGAGATACT 
GGAGAAAAGC TGACAGTTGC TGAAAATGAG GCAGAGACTA AACTTCAAGC TATTTTGGAA 
GACATCCAGG TCACCCTTTT CACAAGGGCT TCTGAAGACC TTAAGACTCA TATGGTTGTG 
GCTAATACAA TGGAAGACTT TCAGAAGATA CTAGATTCTG GAAAGATTGT TCAGATTCCA 
TTCTGTGGGG AAATTGACTG TGAGGACTGG ATCAAAAAGA CCACTGCCAG GGATCAAGAT 
CTTGAACCTG GTGCTCCATC CATGGGAGCT AAAAGCCTTT GCATCCCCTT CAAACCACTC 
TGTGAACTGC AGCCTGGAGC CAAATGTGTC TGTGGCAAGA ACCCTGCCAA GTACTACACC 
TTATTTGGTC GCAGCTACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016856103.1
CDS	105..3065
Translation	MKEVAKHPKNPEVGLKPVWYSPKVFIEGADAETFSEGEMVTFINWGNLNITKIHKNADGKIISLDAKLNLENKDYKKTTKVTWLAETTHALPIPVICVTYEHLITKPVLGKDEDFKQYVNKNSKHEELMLGDPCLKDLKKGDIIQLQRRGFFICDQPYEPVSPYSCKEAPCVLIYIPDGHTKEMPTSGSKEKTKVEATKNETSAPFKERPTPSLNNNCTTSEDSLVLYNRVAVQGDVVRELKAKKAPKEDVDAAVKQLLSLKAEYKEKTGQEYKPGNPPAEIGQNISSNSSASILESKSLYDEVAAQGEVVRKLKAEKSPKAKINEAVECLLSLKAQYKEKTGKEYIPGQPPLSQSSDSSPTRNSEPAGLETPEAKVLFDKVASQGEVVRKLKTEKAPKDQVDIAVQELLQLKAQYKSLIGVEYKPVSATGAEDKDKKKKEKENKSEKQNKPQKQNDGQRKDPSKNQGGGLSSSGAGEGQGPKKQTRLGLEAKKEENLADWYSQVITKSEMIEYHDISGCYILRPWAYAIWEAIKDFFDAEIKKLGVENCYFPMFVSQSALEKEKTHVADFAPEVAWVTRSGKTELAEPIAIRPTSETVMYPAYAKWVQSHRDLPIKLNQWCNVVRWEFKHPQPFLRTREFLWQEGHSAFATMEEAAEEVLQILDLYAQVYEELLAIPVVKGRKTEKEKFAGGDYTTTIEAFISASGRAIQGGTSHHLGQNFSKMFEIVFEDPKIPGEKQFAYQNSWGLTTRTIGVMTMVHGDNMGLVLPPRVACVQVVIIPCGITNALSEEDKEALIAKCNDYRRRLLSVNIRVRADLRDNYSPGWKFNHWELKGVPIRLEVGPRDMKSCQFVAVRRDTGEKLTVAENEAETKLQAILEDIQVTLFTRASEDLKTHMVVANTMEDFQKILDSGKIVQIPFCGEIDCEDWIKKTTARDQDLEPGAPSMGAKSLCIPFKPLCELQPGAKCVCGKNPAKYYTLFGRSY

Target ORF information:

RefSeq Version	XM_017000614.1
Organism	Homo sapiens(human)
Definition	Homo sapiens glutamyl-prolyl-tRNA synthetase (EPRS), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017000614.1

ORF Insert Sequence:

ATGAAAGAAG TAGCCAAACA CCCAAAGAAT CCTGAGGTTG GCTTGAAGCC TGTGTGGTAT 
AGTCCCAAAG TTTTCATTGA AGGTGCTGAT GCAGAGACTT TTTCGGAGGG TGAGATGGTT 
ACATTTATAA ATTGGGGCAA CCTCAACATT ACAAAAATAC ACAAAAATGC AGATGGAAAA 
ATCATATCTC TTGATGCAAA GTTGAATTTG GAAAACAAAG ACTACAAGAA AACCACTAAG 
GTCACTTGGC TTGCAGAGAC TACACATGCT CTTCCTATTC CAGTAATCTG TGTCACTTAT 
GAGCACTTGA TCACAAAGCC AGTGCTAGGA AAAGACGAGG ACTTTAAGCA GTATGTCAAC 
AAGAACAGTA AGCATGAAGA GCTAATGCTA GGGGATCCCT GCCTTAAGGA TTTGAAAAAA 
GGAGATATTA TACAACTCCA GAGAAGAGGA TTCTTCATAT GTGATCAACC TTATGAACCT 
GTTAGCCCAT ATAGTTGCAA GGAAGCCCCG TGTGTTTTGA TATACATTCC TGATGGGCAC 
ACAAAGGAAA TGCCAACATC AGGGTCAAAG GAAAAGACCA AAGTAGAAGC CACAAAAAAT 
GAGACCTCTG CTCCTTTTAA GGAAAGACCA ACACCTTCTC TGAATAATAA TTGTACTACA 
TCTGAGGATT CCTTGGTCCT TTACAATAGA GTGGCTGTTC AAGGAGATGT GGTTCGTGAA 
TTAAAAGCCA AGAAAGCACC AAAGGAAGAT GTAGATGCAG CTGTAAAACA GCTTTTGTCT 
TTGAAAGCTG AATATAAGGA GAAAACTGGC CAGGAATATA AACCTGGAAA CCCTCCTGCT 
GAAATAGGAC AGAATATTTC TTCTAATTCC TCAGCAAGTA TTCTGGAAAG TAAATCTCTG 
TATGATGAAG TTGCTGCACA AGGGGAGGTG GTTCGTAAGC TAAAAGCTGA AAAATCCCCT 
AAGGCTAAAA TAAATGAAGC TGTAGAATGC TTACTGTCCC TGAAGGCTCA GTATAAAGAA 
AAAACTGGGA AGGAGTACAT ACCTGGTCAG CCCCCATTAT CTCAAAGTTC GGATTCAAGC 
CCAACCAGAA ATTCTGAACC TGCTGGTTTA GAAACACCAG AAGCGAAAGT ACTTTTTGAC 
AAAGTAGCTT CTCAAGGGGA AGTAGTTCGG AAACTTAAAA CTGAAAAAGC CCCTAAGGAT 
CAAGTAGATA TAGCTGTTCA AGAACTCCTT CAGCTAAAGG CACAGTACAA GTCTTTGATA 
GGAGTAGAGT ATAAGCCTGT GTCGGCCACT GGAGCTGAGG ACAAAGATAA GAAGAAGAAA 
GAAAAAGAAA ATAAATCTGA AAAGCAGAAT AAGCCTCAGA AACAAAATGA TGGCCAAAGG 
AAAGACCCTT CTAAAAACCA AGGAGGTGGG CTCTCATCAA GTGGAGCAGG AGAAGGGCAG 
GGGCCTAAGA AACAGACCAG GTTGGGTCTT GAGGCAAAAA AAGAAGAAAA TCTTGCTGAT 
TGGTATTCTC AGGTCATCAC AAAGTCAGAA ATGATTGAAT ACCATGACAT AAGTGGCTGT 
TATATTCTTC GTCCCTGGGC CTATGCCATT TGGGAAGCCA TCAAGGACTT TTTTGATGCT 
GAGATCAAGA AACTTGGTGT TGAAAACTGC TACTTCCCCA TGTTTGTGTC TCAAAGTGCA 
TTAGAGAAAG AGAAGACTCA TGTTGCTGAC TTTGCCCCAG AGGTTGCTTG GGTTACAAGA 
TCTGGCAAAA CCGAGCTGGC AGAACCAATT GCCATTCGTC CTACTAGTGA AACAGTAATG 
TATCCTGCAT ATGCAAAATG GGTACAGTCA CACAGAGACC TGCCCATCAA GCTCAATCAG 
TGGTGCAATG TGGTGCGTTG GGAATTCAAG CATCCTCAGC CTTTCCTACG TACTCGTGAA 
TTTCTTTGGC AGGAAGGGCA CAGTGCTTTT GCTACCATGG AAGAGGCAGC GGAAGAGGTC 
TTGCAGATAC TTGACTTATA TGCTCAGGTA TATGAAGAAC TCCTGGCAAT TCCTGTTGTT 
AAAGGAAGAA AGACGGAAAA GGAAAAATTT GCAGGAGGAG ACTATACAAC TACAATAGAA 
GCATTTATAT CTGCTAGTGG AAGAGCTATC CAGGGAGGAA CATCACATCA TTTAGGGCAG 
AATTTTTCCA AAATGTTTGA AATCGTTTTT GAAGATCCAA AGATACCAGG AGAGAAGCAA 
TTTGCCTATC AAAACTCCTG GGGCCTGACA ACTCGAACTA TTGGTGTTAT GACCATGGTT 
CATGGGGACA ACATGGGTTT AGTATTACCA CCCCGTGTAG CATGTGTTCA GGTGGTGATT 
ATTCCTTGTG GCATTACCAA TGCACTTTCT GAAGAAGACA AAGAAGCGCT GATTGCAAAA 
TGCAATGATT ATCGAAGGCG ATTACTCAGT GTTAACATCC GCGTTAGAGC TGATTTACGA 
GATAATTATT CTCCAGGTTG GAAATTCAAT CACTGGGAGC TCAAGGGAGT TCCCATTAGA 
CTTGAAGTTG GGCCACGTGA TATGAAGAGC TGTCAGTTTG TAGCCGTCAG ACGAGATACT 
GGAGAAAAGC TGACAGTTGC TGAAAATGAG GCAGAGACTA AACTTCAAGC TATTTTGGAA 
GACATCCAGG TCACCCTTTT CACAAGGGCT TCTGAAGACC TTAAGACTCA TATGGTTGTG 
GCTAATACAA TGGAAGACTT TCAGAAGATA CTAGATTCTG GAAAGATTGT TCAGATTCCA 
TTCTGTGGGG AAATTGACTG TGAGGACTGG ATCAAAAAGA CCACTGCCAG GGATCAAGAT 
CTTGAACCTG GTGCTCCATC CATGGGAGCT AAAAGCCTTT GCATCCCCTT CAAACCACTC 
TGTGAACTGC AGCCTGGAGC CAAATGTGTC TGTGGCAAGA ACCCTGCCAA GTACTACACC 
TTATTTGGTC GCAGCTACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A Single Adaptable Cochaperone-Scaffold Complex Delivers Nascent Iron-Sulfur Clusters to Mammalian Respiratory Chain Complexes I-III.
Cell metabolism25(4)945-953.e6(2017 Apr)
Maio N,Kim KS,Singh A,Rouault TA

EPRS is a critical mTORC1-S6K1 effector that influences adiposity in mice.
Nature542(7641)357-361(2017 02)
Arif A,Terenzi F,Potdar AA,Jia J,Sacks J,China A,Halawani D,Vasu K,Li X,Brown JM,Chen J,Kozma SC,Thomas G,Fox PL

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EPRS cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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