ENDOG cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	ENDOG
Entrez Gene ID	2021
Full Name	endonuclease G
General protein information	Preferred Names endonuclease G Names endonuclease G, mitochondrial endo G mitochondrial endonuclease G
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 9 Map Location: 9q34.11
Summary	The protein encoded by this gene is a nuclear encoded endonuclease that is localized in the mitochondrion. The encoded protein is widely distributed among animals and cleaves DNA at GC tracts. This protein is capable of generating the RNA primers required by DNA polymerase gamma to initiate replication of mitochondrial DNA. [provided by RefSeq, Jul 2008].
Disorder MIM:	600440

mRNA and Protein(s)

mRNA	Protein	Name
NM_004435.2	NP_004426.2	endonuclease G, mitochondrial precursor
XM_011518347.2	XP_011516649.1	endonuclease G, mitochondrial isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	ENDOG	XP_003312364.2
Bos taurus (cattle)	ENDOG	NP_787017.1
Gallus gallus (chicken)	ENDOG	XP_415487.2
Drosophila melanogaster (fruit fly)	EndoG	NP_610737.1
Caenorhabditis elegans (roundworm)	cps-6	NP_491371.1
Kluyveromyces lactis	KLLA0D00440g	XP_453091.1
Eremothecium gossypii	AGOS_ADR144C	NP_984240.1
Magnaporthe oryzae (rice blast fungus)	MGG_05324	XP_003712888.1
Homo sapiens (human)	ENDOG	NP_004426.2
Gallus gallus (chicken)	LOC100859613	XP_004945896.1
Danio rerio (zebrafish)	endog	NP_001019385.1
Neurospora crassa	NCU00030	XP_957017.1
	LOC717171	XP_001110294.1
Rattus norvegicus (Norway rat)	Endog	NP_001030110.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007845	XP_317654.4
Canis lupus familiaris (dog)	ENDOG	XP_851160.2
Mus musculus (house mouse)	Endog	NP_031957.1
Saccharomyces cerevisiae (baker's yeast)	NUC1	NP_012327.1
Xenopus tropicalis (tropical clawed frog)	endog	NP_001017202.1
Schizosaccharomyces pombe (fission yeast)	pnu1	NP_594598.2

Gene Ontology
Bibliography

Related articles in PubMed

Alternative splicing of telomerase catalytic subunit hTERT generated by apoptotic endonuclease EndoG induces human CD4
Zhdanov DD, Vasina DA, Grachev VA, Orlova EV, Orlova VS, Pokrovskaya MV, Alexandrova SS, Sokolov NN
European journal of cell biology96(7)653-664(2017 Oct)

Apoptotic Endonuclease EndoG Inhibits Telomerase Activity and Induces Malignant Transformation of Human CD4+ T Cells.
Vasina DA, Zhdanov DD, Orlova EV, Orlova VS, Pokrovskaya MV, Aleksandrova SS, Sokolov NN
Biochemistry. Biokhimiia82(1)24-37(2017 Jan)

Galectin-3 promotes caspase-independent cell death of HIV-1-infected macrophages.
Xue J, Fu C, Cong Z, Peng L, Peng Z, Chen T, Wang W, Jiang H, Wei Q, Qin C
The FEBS journal284(1)97-113(2017 01)

[Apoptotic endonuclease EndoG regulates alternative splicing of human telomerase catalytic subunit hTERT].
Zhdanov DD, Vasina DA, Orlova EV, Orlova VS, Pokrovskaya MV, Aleksandrova SS, Sokolov NN
Biomeditsinskaia khimiia62(5)544-554(2016 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Overexpression of EndoG in CD4+ T cells downregulated the expression of the active full-length hTERT variant and upregulated the inactive alternatively spliced variant.
Title: Apoptotic Endonuclease EndoG Inhibits Telomerase Activity and Induces Malignant Transformation of Human CD4+ T Cells.

Galectin-3-induced cell death in HIV-1-infected macrophages is most likely related to the translocation of Endo G from the cytoplasm to the nucleus.
Title: Galectin-3 promotes caspase-independent cell death of HIV-1-infected macrophages.

ndoG digests long non-coding RNA and produces 47-mer RNA oligonucleotide complementary to hTERT pre-mRNA exon 8 and intron 8 junction place. Interaction of 47-mer RNA oligonucleotide and hTERT pre-mRNA causes alternative splicing.
Title: [Apoptotic endonuclease EndoG regulates alternative splicing of human telomerase catalytic subunit hTERT].

Overexpression of EndoG in capital ES, Cyrillicsmall a, Cyrilliccapital ES, Cyrillicsmall o, Cyrillic-2 cells downregulated the expression of active full-length hTERT variant and upregulated non-active spliced variant.
Title: [Apoptotic endonuclease EndoG induces alternative splicing of telomerase catalytic subunit hTERT and death of tumor cells].

EndoG is not a mediator of exogenous DNA clearance, but in non-physiological circumstances, it may nonspecifically cleave intracellular DNA regardless of its origin.
Title: Role of Endonuclease G in Exogenous DNA Stability in HeLa Cells.

The following ENDOG gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ENDOG cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OHu25388	NM_004435.2 Latest version!	Homo sapiens endonuclease G (ENDOG), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$99.50-$139.30 $199.00
OHu57503	XM_011518347.2 Latest version!	Homo sapiens endonuclease G (ENDOG), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$223.30 $319.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ENDOG ( NM_004435.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_004426.2 Homo sapiens endonuclease G (ENDOG), mRNA.

Price & Availability↑

CloneID	OHu25388
Clone ID Related Accession (Same CDS sequence)	NM_004435.2
Accession Version	NM_004435.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 894bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-20
Organism	Homo sapiens(human)
Product	endonuclease G, mitochondrial precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC016351.1, X79444.1 and BC004922.2. This sequence is a reference standard in the RefSeqGene project. On Oct 4, 2004 this sequence version replaced NM_004435.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC016351.1, SRR1163658.345369.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta MANE Ensembl match :: ENST00000372642.5/ ENSP00000361725.4 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCGGGCGC TGCGGGCCGG CCTGACCCTG GCGTCGGGCG CGGGGCTGGG TGCGGTCGTC 
GAGGGCTGGC GGCGGCGGCG GGAGGACGCG CGGGCGGCGC CGGGACTGCT GGGCCGGCTG 
CCCGTGCTGC CCGTGGCGGC GGCAGCCGAG TTGCCCCCTG TGCCCGGGGG ACCCCGCGGC 
CCGGGCGAGC TGGCCAAGTA CGGGCTGCCG GGGCTGGCGC AGCTCAAGAG CCGCGAGTCG 
TACGTGCTGT GCTACGACCC GCGCACCCGC GGCGCGCTCT GGGTGGTGGA GCAGCTGCGA 
CCCGAGCGTC TCCGCGGCGA CGGCGACCGG CGCGAGTGCG ACTTCCGCGA GGACGACTCG 
GTGCACGCGT ACCACCGTGC CACCAACGCC GACTACCGCG GCAGTGGCTT CGACCGCGGT 
CACCTGGCCG CCGCCGCCAA CCACCGCTGG AGCCAGAAGG CCATGGACGA CACGTTCTAC 
CTGAGCAACG TCGCGCCCCA GGTGCCCCAC CTCAACCAGA ATGCCTGGAA CAACCTGGAG 
AAATATAGCC GCAGCTTGAC CCGCAGCTAC CAAAACGTCT ATGTCTGCAC AGGGCCACTC 
TTCCTGCCCA GGACAGAGGC TGATGGGAAA TCCTACGTAA AGTACCAGGT CATCGGCAAG 
AACCACGTGG CAGTGCCCAC ACACTTCTTC AAGGTGCTGA TCCTGGAGGC AGCAGGTGGG 
CAAATTGAGC TCCGCACCTA CGTGATGCCC AACGCACCTG TGGATGAGGC CATCCCACTG 
GAGCGCTTCC TGGTGCCCAT CGAGAGCATT GAGCGGGCTT CGGGGCTGCT CTTTGTGCCA 
AACATCCTGG CGCGGGCAGG CAGCCTCAAG GCCATCACGG CGGGCAGTAA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004426.2
CDS	186..1079
Translation	MRALRAGLTLASGAGLGAVVEGWRRRREDARAAPGLLGRLPVLPVAAAAELPPVPGGPRGPGELAKYGLPGLAQLKSRESYVLCYDPRTRGALWVVEQLRPERLRGDGDRRECDFREDDSVHAYHRATNADYRGSGFDRGHLAAAANHRWSQKAMDDTFYLSNVAPQVPHLNQNAWNNLEKYSRSLTRSYQNVYVCTGPLFLPRTEADGKSYVKYQVIGKNHVAVPTHFFKVLILEAAGGQIELRTYVMPNAPVDEAIPLERFLVPIESIERASGLLFVPNILARAGSLKAITAGSK

Target ORF information:

RefSeq Version	NM_004435.2
Organism	Homo sapiens(human)
Definition	Homo sapiens endonuclease G (ENDOG), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004435.2

ORF Insert Sequence:

ATGCGGGCGC TGCGGGCCGG CCTGACCCTG GCGTCGGGCG CGGGGCTGGG TGCGGTCGTC 
GAGGGCTGGC GGCGGCGGCG GGAGGACGCG CGGGCGGCGC CGGGACTGCT GGGCCGGCTG 
CCCGTGCTGC CCGTGGCGGC GGCAGCCGAG TTGCCCCCTG TGCCCGGGGG ACCCCGCGGC 
CCGGGCGAGC TGGCCAAGTA CGGGCTGCCG GGGCTGGCGC AGCTCAAGAG CCGCGAGTCG 
TACGTGCTGT GCTACGACCC GCGCACCCGC GGCGCGCTCT GGGTGGTGGA GCAGCTGCGA 
CCCGAGCGTC TCCGCGGCGA CGGCGACCGG CGCGAGTGCG ACTTCCGCGA GGACGACTCG 
GTGCACGCGT ACCACCGTGC CACCAACGCC GACTACCGCG GCAGTGGCTT CGACCGCGGT 
CACCTGGCCG CCGCCGCCAA CCACCGCTGG AGCCAGAAGG CCATGGACGA CACGTTCTAC 
CTGAGCAACG TCGCGCCCCA GGTGCCCCAC CTCAACCAGA ATGCCTGGAA CAACCTGGAG 
AAATATAGCC GCAGCTTGAC CCGCAGCTAC CAAAACGTCT ATGTCTGCAC AGGGCCACTC 
TTCCTGCCCA GGACAGAGGC TGATGGGAAA TCCTACGTAA AGTACCAGGT CATCGGCAAG 
AACCACGTGG CAGTGCCCAC ACACTTCTTC AAGGTGCTGA TCCTGGAGGC AGCAGGTGGG 
CAAATTGAGC TCCGCACCTA CGTGATGCCC AACGCACCTG TGGATGAGGC CATCCCACTG 
GAGCGCTTCC TGGTGCCCAT CGAGAGCATT GAGCGGGCTT CGGGGCTGCT CTTTGTGCCA 
AACATCCTGG CGCGGGCAGG CAGCCTCAAG GCCATCACGG CGGGCAGTAA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ENDOG ( XM_011518347.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011516649.1 Homo sapiens endonuclease G (ENDOG), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu57503
Clone ID Related Accession (Same CDS sequence)	XM_011518347.2
Accession Version	XM_011518347.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1017bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	endonuclease G, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011518347.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGCCA ATCGCGAGCT GAGGCGACGA TGCCACGCCC CTCATACCGG CGTCTTAAAT 
GCGCAAAGAA AGGACGCCGG GGACACCCGG TTGGGCTCTG CTGCTCCCTT CTGGGTTCCG 
AGGCCCAAGC CCTTGGCAGT GTTTGTGAGT GGAAGGGAGG TCACGCTATC GTCCGCGGCC 
CCAGCAGCCC TGTGCCCTCG TTGGATCCCG CGACGCGGCT CCTTTAAGAG CCTCGCGGGT 
CGCCCGCCGC TAGGTCGCTC CCCGGCCATG CGGGCGCTGC GGGCCGGCCT GACCCTGGCG 
TCGGGCGCGG GGCTGGGTGC GGTCGTCGAG GGCTGGCGGC GGCGGCGGGA GGACGCGCGG 
GCGGCGCCGG GACTGCTGGG CCGGCTGCCC GTGCTGCCCG TGGCGGCGGC AGCCGAGTTG 
CCCCCTGTGC CCGGGGGACC CCGCGGCCCG GGCGAGCTGG CCAAGTACGG GCTGCCGGGG 
CTGGCGCAGC TCAAGAGCCG CGAGTCGTAC GTGCTGTGCT ACGACCCGCG CACCCGCGGC 
GCGCTCTGGG TGGTGGAGCA GCTGCGACCC GAGCGTCTCC GCGGCGACGG CGACCGGCGC 
GAGTGCGACT TCCGCGAGGA CGACTCGGTG CACGCGTACC ACCGTGCCAC CAACGCCGAC 
TACCGCGGCA GTGGCTTCGA CCGCGGTCAC CTGGCCGCCG CCGCCAACCA CCGCTGGAGC 
CAGAAGGCCA TGGACGACAC GTTCTACCTG AGCAACGTCG CGCCCCAGGT GCCCCACCTC 
AACCAGAATG CCTGGAACAA CCTGGAGAAA TATAGCCGCA GCTTGACCCG CAGCTACCAA 
AACGTCTATG TCTGCACAGG GCCACTCTTC CTGCCCAGGC TCGGGATGGA GTCCAGGCCT 
CATCACCTAG TTCCTTGGTG CTGTGGGCAA ATGACCTGTG TCCCCCTCTG CAAAACCTGT 
GACCTCTTGG TTGATGTCAC ATGCTTCCCC AGGGCCTGGT CCATGGACAG AGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011516649.1
CDS	17..1033
Translation	MVANRELRRRCHAPHTGVLNAQRKDAGDTRLGSAAPFWVPRPKPLAVFVSGREVTLSSAAPAALCPRWIPRRGSFKSLAGRPPLGRSPAMRALRAGLTLASGAGLGAVVEGWRRRREDARAAPGLLGRLPVLPVAAAAELPPVPGGPRGPGELAKYGLPGLAQLKSRESYVLCYDPRTRGALWVVEQLRPERLRGDGDRRECDFREDDSVHAYHRATNADYRGSGFDRGHLAAAANHRWSQKAMDDTFYLSNVAPQVPHLNQNAWNNLEKYSRSLTRSYQNVYVCTGPLFLPRLGMESRPHHLVPWCCGQMTCVPLCKTCDLLVDVTCFPRAWSMDRG

Target ORF information:

RefSeq Version	XM_011518347.2
Organism	Homo sapiens(human)
Definition	Homo sapiens endonuclease G (ENDOG), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011518347.2

ORF Insert Sequence:

ATGGTGGCCA ATCGCGAGCT GAGGCGACGA TGCCACGCCC CTCATACCGG CGTCTTAAAT 
GCGCAAAGAA AGGACGCCGG GGACACCCGG TTGGGCTCTG CTGCTCCCTT CTGGGTTCCG 
AGGCCCAAGC CCTTGGCAGT GTTTGTGAGT GGAAGGGAGG TCACGCTATC GTCCGCGGCC 
CCAGCAGCCC TGTGCCCTCG TTGGATCCCG CGACGCGGCT CCTTTAAGAG CCTCGCGGGT 
CGCCCGCCGC TAGGTCGCTC CCCGGCCATG CGGGCGCTGC GGGCCGGCCT GACCCTGGCG 
TCGGGCGCGG GGCTGGGTGC GGTCGTCGAG GGCTGGCGGC GGCGGCGGGA GGACGCGCGG 
GCGGCGCCGG GACTGCTGGG CCGGCTGCCC GTGCTGCCCG TGGCGGCGGC AGCCGAGTTG 
CCCCCTGTGC CCGGGGGACC CCGCGGCCCG GGCGAGCTGG CCAAGTACGG GCTGCCGGGG 
CTGGCGCAGC TCAAGAGCCG CGAGTCGTAC GTGCTGTGCT ACGACCCGCG CACCCGCGGC 
GCGCTCTGGG TGGTGGAGCA GCTGCGACCC GAGCGTCTCC GCGGCGACGG CGACCGGCGC 
GAGTGCGACT TCCGCGAGGA CGACTCGGTG CACGCGTACC ACCGTGCCAC CAACGCCGAC 
TACCGCGGCA GTGGCTTCGA CCGCGGTCAC CTGGCCGCCG CCGCCAACCA CCGCTGGAGC 
CAGAAGGCCA TGGACGACAC GTTCTACCTG AGCAACGTCG CGCCCCAGGT GCCCCACCTC 
AACCAGAATG CCTGGAACAA CCTGGAGAAA TATAGCCGCA GCTTGACCCG CAGCTACCAA 
AACGTCTATG TCTGCACAGG GCCACTCTTC CTGCCCAGGC TCGGGATGGA GTCCAGGCCT 
CATCACCTAG TTCCTTGGTG CTGTGGGCAA ATGACCTGTG TCCCCCTCTG CAAAACCTGT 
GACCTCTTGG TTGATGTCAC ATGCTTCCCC AGGGCCTGGT CCATGGACAG AGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Alternative splicing of telomerase catalytic subunit hTERT generated by apoptotic endonuclease EndoG induces human CD4
European journal of cell biology96(7)653-664(2017 Oct)
Zhdanov DD,Vasina DA,Grachev VA,Orlova EV,Orlova VS,Pokrovskaya MV,Alexandrova SS,Sokolov NN

Apoptotic Endonuclease EndoG Inhibits Telomerase Activity and Induces Malignant Transformation of Human CD4+ T Cells.
Biochemistry. Biokhimiia82(1)24-37(2017 Jan)
Vasina DA,Zhdanov DD,Orlova EV,Orlova VS,Pokrovskaya MV,Aleksandrova SS,Sokolov NN

Galectin-3 promotes caspase-independent cell death of HIV-1-infected macrophages.
The FEBS journal284(1)97-113(2017 01)
Xue J,Fu C,Cong Z,Peng L,Peng Z,Chen T,Wang W,Jiang H,Wei Q,Qin C

[Apoptotic endonuclease EndoG regulates alternative splicing of human telomerase catalytic subunit hTERT].
Biomeditsinskaia khimiia62(5)544-554(2016 Jul)
Zhdanov DD,Vasina DA,Orlova EV,Orlova VS,Pokrovskaya MV,Aleksandrova SS,Sokolov NN

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ENDOG cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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