EN2 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	EN2
Entrez Gene ID	2020
Full Name	engrailed homeobox 2
General protein information	Preferred Names engrailed homeobox 2 Names homeobox protein engrailed-2 engrailed homolog 2 engrailed-2 homeobox protein en-2 hu-En-2
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 7 Map Location: 7q36.3
Summary	Homeobox-containing genes are thought to have a role in controlling development. In Drosophila, the 'engrailed' (en) gene plays an important role during development in segmentation, where it is required for the formation of posterior compartments. Different mutations in the mouse homologs, En1 and En2, produced different developmental defects that frequently are lethal. The human engrailed homologs 1 and 2 encode homeodomain-containing proteins and have been implicated in the control of pattern formation during development of the central nervous system. [provided by RefSeq, Jul 2008].
Disorder MIM:	131310

mRNA and Protein(s)

mRNA	Protein	Name
NM_001427.3	NP_001418.2	homeobox protein engrailed-2
NM_001427.4	NP_001418.2	homeobox protein engrailed-2

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	LOC100859435	NP_001254648.1
Danio rerio (zebrafish)	eng2a	NP_571119.2
Xenopus tropicalis (tropical clawed frog)	LOC100498251	XP_002932525.1
Pan troglodytes (chimpanzee)	EN2	XP_001146034.2
Mus musculus (house mouse)	En2	NP_034264.1
Danio rerio (zebrafish)	eng2b	NP_571115.1
Homo sapiens (human)	EN2	NP_001418.2
	EN2	XP_001106130.1
Rattus norvegicus (Norway rat)	En2	NP_001102684.1

Gene Ontology
Bibliography

Related articles in PubMed

Down-regulation of miR-605 promotes the proliferation and invasion of prostate cancer cells by up-regulating EN2.
Zhou YJ, Yang HQ, Xia W, Cui L, Xu RF, Lu H, Xue Z, Zhang B, Tian ZN, Cao YJ, Xing ZY, Yin S, He XZ
Life sciences1907-14(2017 Dec)

The biological functions and mechanism of miR?212 in prostate cancer proliferation, migration and invasion via targeting Engrailed-2.
Zhou Y, Ji Z, Yan W, Zhou Z, Li H
Oncology reports38(3)1411-1419(2017 Sep)

Biological function and mechanism of miR-33a in prostate cancer survival and metastasis: via downregulating Engrailed-2.
Li Q, Lu S, Li X, Hou G, Yan L, Zhang W, Qiao B
Clinical & translational oncology : official publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico19(5)562-570(2017 May)

Hsa-miR-27b is up-regulated in cytomegalovirus-infected human glioma cells, targets engrailed-2 and inhibits its expression.
Wang L, Yang M, Liao S, Liu W, Dai G, Wu G, Chen L
Experimental biology and medicine (Maywood, N.J.)1535370217699535(2017 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

EN2 is negatively regulated by miR-605, and down-regulation of miR-605 promotes the proliferation and invasion of prostate cancer (PCa) cells by up-regulating EN2, which leads to PCa development and progression
Title: Down-regulation of miR-605 promotes the proliferation and invasion of prostate cancer cells by up-regulating EN2.

Our data suggest that the functional interaction of miR-33a and EN-2 is involved in tumorigenesis of prostate cancer
Title: Biological function and mechanism of miR-33a in prostate cancer survival and metastasis: via downregulating Engrailed-2.

We identified a novel heterozygous missense variant (c.425C>G, p.Pro142Arg) in the Engrailed 2 (EN2) gene in two patients. The G variant has never been reported in public databases and was absent in 100 Thai patients with ASD and 435 Thai controls. A case-control study showed that the G allele of c.425C>G was significantly associated with ASD
Title: Whole-exome sequencing identifies a novel heterozygous missense variant of the EN2 gene in two unrelated patients with autism spectrum disorder.

the results demonstrated that EN2 might play an anti-oncogenic role in oncogenesis and development of CCRCC, thereby maintaining the normal growth of human renal tubular epithelial cells.
Title: Engrailed-2 might play an anti-oncogenic role in clear-cell renal cell carcinoma.

EN2 may be a candidate oncogene in bladder cancer by activating the PI3K/Akt pathway and inhibiting PTEN.
Title: Repression of engrailed 2 inhibits the proliferation and invasion of human bladder cancer in vitro and in vivo.

The following EN2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EN2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu17809	NM_001427.4 Latest version!	Homo sapiens engrailed homeobox 2 (EN2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
View Old Accession Versions >>
OHu17809	NM_001427.3	Homo sapiens engrailed homeobox 2 (EN2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

EN2 ( NM_001427.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001418.2 Homo sapiens engrailed homeobox 2 (EN2), mRNA.

Price & Availability↑

CloneID	OHu17809
Clone ID Related Accession (Same CDS sequence)	NM_001427.3 , NM_001427.4
Accession Version	NM_001427.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1002bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-19
Organism	Homo sapiens(human)
Product	homeobox protein engrailed-2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC008060.5 and H16179.1. This sequence is a reference standard in the RefSeqGene project. On Feb 23, 2007 this sequence version replaced NM_001427.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC104970.1, SRR1660805.106245.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2142680 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGGAGA ATGACCCCAA GCCTGGCGAA GCAGCGGCGG CGGTGGAGGG ACAGCGGCAG 
CCGGAATCCA GCCCCGGCGG CGGCTCGGGC GGCGGCGGCG GTAGCAGCCC GGGCGAAGCG 
GACACCGGGC GCCGGCGGGC TCTGATGCTG CCCGCGGTCC TGCAGGCGCC CGGCAACCAC 
CAGCACCCGC ACCGCATCAC CAACTTCTTC ATCGACAACA TCCTGCGGCC CGAGTTCGGC 
CGGCGAAAGG ACGCGGGGAC CTGCTGTGCG GGCGCGGGAG GAGGAAGGGG CGGCGGAGCC 
GGCGGCGAAG GCGGCGCGAG CGGTGCGGAG GGAGGCGGCG GCGCGGGCGG CTCGGAGCAG 
CTCTTGGGCT CGGGCTCCCG AGAGCCCCGG CAGAACCCGC CATGTGCGCC CGGCGCGGGC 
GGGCCGCTCC CAGCCGCCGG CAGCGACTCT CCGGGTGACG GGGAAGGCGG CTCCAAGACG 
CTCTCGCTGC ACGGTGGCGC CAAGAAAGGC GGCGACCCCG GCGGCCCCCT GGACGGGTCG 
CTCAAGGCCC GCGGCTTGGG CGGCGGCGAC CTGTCGGTGA GCTCGGACTC GGACAGCTCG 
CAAGCCGGCG CCAACCTGGG CGCGCAGCCC ATGCTCTGGC CGGCGTGGGT CTACTGTACG 
CGCTACTCGG ACCGGCCTTC TTCAGGTCCC AGGTCTCGAA AACCAAAGAA GAAGAACCCG 
AACAAAGAGG ACAAGCGGCC GCGCACGGCC TTTACCGCCG AGCAGCTGCA GAGGCTCAAG 
GCCGAGTTCC AGACCAACAG GTACCTGACG GAGCAGCGGC GCCAGAGCCT GGCGCAGGAG 
CTGAGCCTCA ACGAGTCACA GATCAAGATT TGGTTCCAGA ACAAGCGCGC CAAGATCAAG 
AAGGCCACGG GCAACAAGAA CACGCTGGCC GTGCACCTCA TGGCACAGGG CTTGTACAAC 
CACTCCACCA CAGCCAAGGA GGGCAAGTCG GACAGCGAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001418.2
CDS	250..1251
Translation	MEENDPKPGEAAAAVEGQRQPESSPGGGSGGGGGSSPGEADTGRRRALMLPAVLQAPGNHQHPHRITNFFIDNILRPEFGRRKDAGTCCAGAGGGRGGGAGGEGGASGAEGGGGAGGSEQLLGSGSREPRQNPPCAPGAGGPLPAAGSDSPGDGEGGSKTLSLHGGAKKGGDPGGPLDGSLKARGLGGGDLSVSSDSDSSQAGANLGAQPMLWPAWVYCTRYSDRPSSGPRSRKPKKKNPNKEDKRPRTAFTAEQLQRLKAEFQTNRYLTEQRRQSLAQELSLNESQIKIWFQNKRAKIKKATGNKNTLAVHLMAQGLYNHSTTAKEGKSDSE

Target ORF information:

RefSeq Version	NM_001427.3
Organism	Homo sapiens(human)
Definition	Homo sapiens engrailed homeobox 2 (EN2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001427.3

ORF Insert Sequence:

ATGGAGGAGA ATGACCCCAA GCCTGGCGAA GCAGCGGCGG CGGTGGAGGG ACAGCGGCAG 
CCGGAATCCA GCCCCGGCGG CGGCTCGGGC GGCGGCGGCG GTAGCAGCCC GGGCGAAGCG 
GACACCGGGC GCCGGCGGGC TCTGATGCTG CCCGCGGTCC TGCAGGCGCC CGGCAACCAC 
CAGCACCCGC ACCGCATCAC CAACTTCTTC ATCGACAACA TCCTGCGGCC CGAGTTCGGC 
CGGCGAAAGG ACGCGGGGAC CTGCTGTGCG GGCGCGGGAG GAGGAAGGGG CGGCGGAGCC 
GGCGGCGAAG GCGGCGCGAG CGGTGCGGAG GGAGGCGGCG GCGCGGGCGG CTCGGAGCAG 
CTCTTGGGCT CGGGCTCCCG AGAGCCCCGG CAGAACCCGC CATGTGCGCC CGGCGCGGGC 
GGGCCGCTCC CAGCCGCCGG CAGCGACTCT CCGGGTGACG GGGAAGGCGG CTCCAAGACG 
CTCTCGCTGC ACGGTGGCGC CAAGAAAGGC GGCGACCCCG GCGGCCCCCT GGACGGGTCG 
CTCAAGGCCC GCGGCTTGGG CGGCGGCGAC CTGTCGGTGA GCTCGGACTC GGACAGCTCG 
CAAGCCGGCG CCAACCTGGG CGCGCAGCCC ATGCTCTGGC CGGCGTGGGT CTACTGTACG 
CGCTACTCGG ACCGGCCTTC TTCAGGTCCC AGGTCTCGAA AACCAAAGAA GAAGAACCCG 
AACAAAGAGG ACAAGCGGCC GCGCACGGCC TTTACCGCCG AGCAGCTGCA GAGGCTCAAG 
GCCGAGTTCC AGACCAACAG GTACCTGACG GAGCAGCGGC GCCAGAGCCT GGCGCAGGAG 
CTGAGCCTCA ACGAGTCACA GATCAAGATT TGGTTCCAGA ACAAGCGCGC CAAGATCAAG 
AAGGCCACGG GCAACAAGAA CACGCTGGCC GTGCACCTCA TGGCACAGGG CTTGTACAAC 
CACTCCACCA CAGCCAAGGA GGGCAAGTCG GACAGCGAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EN2 ( NM_001427.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_001418.2 Homo sapiens engrailed homeobox 2 (EN2), mRNA.

Price & Availability↑

CloneID	OHu17809
Clone ID Related Accession (Same CDS sequence)	NM_001427.3 , NM_001427.4
Accession Version	NM_001427.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1002bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-27
Organism	Homo sapiens(human)
Product	homeobox protein engrailed-2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC008060.5 and H16179.1. This sequence is a reference standard in the RefSeqGene project. On May 17, 2019 this sequence version replaced NM_001427.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.106245.1, SRR1660807.97961.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2142680 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000297375.4/ ENSP00000297375.4 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGAGGAGA ATGACCCCAA GCCTGGCGAA GCAGCGGCGG CGGTGGAGGG ACAGCGGCAG 
CCGGAATCCA GCCCCGGCGG CGGCTCGGGC GGCGGCGGCG GTAGCAGCCC GGGCGAAGCG 
GACACCGGGC GCCGGCGGGC TCTGATGCTG CCCGCGGTCC TGCAGGCGCC CGGCAACCAC 
CAGCACCCGC ACCGCATCAC CAACTTCTTC ATCGACAACA TCCTGCGGCC CGAGTTCGGC 
CGGCGAAAGG ACGCGGGGAC CTGCTGTGCG GGCGCGGGAG GAGGAAGGGG CGGCGGAGCC 
GGCGGCGAAG GCGGCGCGAG CGGTGCGGAG GGAGGCGGCG GCGCGGGCGG CTCGGAGCAG 
CTCTTGGGCT CGGGCTCCCG AGAGCCCCGG CAGAACCCGC CATGTGCGCC CGGCGCGGGC 
GGGCCGCTCC CAGCCGCCGG CAGCGACTCT CCGGGTGACG GGGAAGGCGG CTCCAAGACG 
CTCTCGCTGC ACGGTGGCGC CAAGAAAGGC GGCGACCCCG GCGGCCCCCT GGACGGGTCG 
CTCAAGGCCC GCGGCTTGGG CGGCGGCGAC CTGTCGGTGA GCTCGGACTC GGACAGCTCG 
CAAGCCGGCG CCAACCTGGG CGCGCAGCCC ATGCTCTGGC CGGCGTGGGT CTACTGTACG 
CGCTACTCGG ACCGGCCTTC TTCAGGTCCC AGGTCTCGAA AACCAAAGAA GAAGAACCCG 
AACAAAGAGG ACAAGCGGCC GCGCACGGCC TTTACCGCCG AGCAGCTGCA GAGGCTCAAG 
GCCGAGTTCC AGACCAACAG GTACCTGACG GAGCAGCGGC GCCAGAGCCT GGCGCAGGAG 
CTGAGCCTCA ACGAGTCACA GATCAAGATT TGGTTCCAGA ACAAGCGCGC CAAGATCAAG 
AAGGCCACGG GCAACAAGAA CACGCTGGCC GTGCACCTCA TGGCACAGGG CTTGTACAAC 
CACTCCACCA CAGCCAAGGA GGGCAAGTCG GACAGCGAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001418.2
CDS	250..1251
Translation	MEENDPKPGEAAAAVEGQRQPESSPGGGSGGGGGSSPGEADTGRRRALMLPAVLQAPGNHQHPHRITNFFIDNILRPEFGRRKDAGTCCAGAGGGRGGGAGGEGGASGAEGGGGAGGSEQLLGSGSREPRQNPPCAPGAGGPLPAAGSDSPGDGEGGSKTLSLHGGAKKGGDPGGPLDGSLKARGLGGGDLSVSSDSDSSQAGANLGAQPMLWPAWVYCTRYSDRPSSGPRSRKPKKKNPNKEDKRPRTAFTAEQLQRLKAEFQTNRYLTEQRRQSLAQELSLNESQIKIWFQNKRAKIKKATGNKNTLAVHLMAQGLYNHSTTAKEGKSDSE

Target ORF information:

RefSeq Version	NM_001427.4
Organism	Homo sapiens(human)
Definition	Homo sapiens engrailed homeobox 2 (EN2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001427.4

ORF Insert Sequence:

ATGGAGGAGA ATGACCCCAA GCCTGGCGAA GCAGCGGCGG CGGTGGAGGG ACAGCGGCAG 
CCGGAATCCA GCCCCGGCGG CGGCTCGGGC GGCGGCGGCG GTAGCAGCCC GGGCGAAGCG 
GACACCGGGC GCCGGCGGGC TCTGATGCTG CCCGCGGTCC TGCAGGCGCC CGGCAACCAC 
CAGCACCCGC ACCGCATCAC CAACTTCTTC ATCGACAACA TCCTGCGGCC CGAGTTCGGC 
CGGCGAAAGG ACGCGGGGAC CTGCTGTGCG GGCGCGGGAG GAGGAAGGGG CGGCGGAGCC 
GGCGGCGAAG GCGGCGCGAG CGGTGCGGAG GGAGGCGGCG GCGCGGGCGG CTCGGAGCAG 
CTCTTGGGCT CGGGCTCCCG AGAGCCCCGG CAGAACCCGC CATGTGCGCC CGGCGCGGGC 
GGGCCGCTCC CAGCCGCCGG CAGCGACTCT CCGGGTGACG GGGAAGGCGG CTCCAAGACG 
CTCTCGCTGC ACGGTGGCGC CAAGAAAGGC GGCGACCCCG GCGGCCCCCT GGACGGGTCG 
CTCAAGGCCC GCGGCTTGGG CGGCGGCGAC CTGTCGGTGA GCTCGGACTC GGACAGCTCG 
CAAGCCGGCG CCAACCTGGG CGCGCAGCCC ATGCTCTGGC CGGCGTGGGT CTACTGTACG 
CGCTACTCGG ACCGGCCTTC TTCAGGTCCC AGGTCTCGAA AACCAAAGAA GAAGAACCCG 
AACAAAGAGG ACAAGCGGCC GCGCACGGCC TTTACCGCCG AGCAGCTGCA GAGGCTCAAG 
GCCGAGTTCC AGACCAACAG GTACCTGACG GAGCAGCGGC GCCAGAGCCT GGCGCAGGAG 
CTGAGCCTCA ACGAGTCACA GATCAAGATT TGGTTCCAGA ACAAGCGCGC CAAGATCAAG 
AAGGCCACGG GCAACAAGAA CACGCTGGCC GTGCACCTCA TGGCACAGGG CTTGTACAAC 
CACTCCACCA CAGCCAAGGA GGGCAAGTCG GACAGCGAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Down-regulation of miR-605 promotes the proliferation and invasion of prostate cancer cells by up-regulating EN2.
Life sciences1907-14(2017 Dec)
Zhou YJ,Yang HQ,Xia W,Cui L,Xu RF,Lu H,Xue Z,Zhang B,Tian ZN,Cao YJ,Xing ZY,Yin S,He XZ

The biological functions and mechanism of miR?212 in prostate cancer proliferation, migration and invasion via targeting Engrailed-2.
Oncology reports38(3)1411-1419(2017 Sep)
Zhou Y,Ji Z,Yan W,Zhou Z,Li H

Biological function and mechanism of miR-33a in prostate cancer survival and metastasis: via downregulating Engrailed-2.
Clinical & translational oncology : official publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico19(5)562-570(2017 May)
Li Q,Lu S,Li X,Hou G,Yan L,Zhang W,Qiao B

Hsa-miR-27b is up-regulated in cytomegalovirus-infected human glioma cells, targets engrailed-2 and inhibits its expression.
Experimental biology and medicine (Maywood, N.J.)1535370217699535(2017 Jan)
Wang L,Yang M,Liao S,Liu W,Dai G,Wu G,Chen L

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EN2 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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