CRYBG1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	CRYBG1
Entrez Gene ID	202
Full Name	crystallin beta-gamma domain containing 1
Synonyms	AIM1,ST4
General protein information	Preferred Names crystallin beta-gamma domain containing 1 Names beta/gamma crystallin domain-containing protein 1 absent in melanoma 1 protein beta-gamma crystallin domain containing 1 suppression of tumorigenicity 4 (malignant melanoma)
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 6 Map Location: 6q21
Disorder MIM:	601797

mRNA and Protein(s)

mRNA	Protein	Name
NM_001624.4	NP_001615.2	beta/gamma crystallin domain-containing protein 1 isoform 2
NM_001371242.1	NP_001358171.1	beta/gamma crystallin domain-containing protein 1 isoform 1
XM_005266839.3	XP_005266896.1	beta/gamma crystallin domain-containing protein 1 isoform X1
NM_001371242.2	NP_001358171.1	beta/gamma crystallin domain-containing protein 1 isoform 1
XM_017010333.1	XP_016865822.1	beta/gamma crystallin domain-containing protein 1 isoform X2
NM_001624.3	NP_001615.2	beta/gamma crystallin domain-containing protein 1 isoform 2

Pathways from BioSystems

Homologies

Xenopus tropicalis (tropical clawed frog)	aim1	XP_004914608.1
Canis lupus familiaris (dog)	AIM1	XP_005627838.1
Homo sapiens (human)	AIM1	NP_001615.1
Pan troglodytes (chimpanzee)	AIM1	XP_518660.3
Rattus norvegicus (Norway rat)	Aim1	XP_006256655.1
Gallus gallus (chicken)	AIM1	XP_004940405.1
	AIM1	XP_001088619.2
Mus musculus (house mouse)	Aim1	NP_765981.2
Bos taurus (cattle)	AIM1	XP_003586516.2

Gene Ontology
Bibliography

Related articles in PubMed

Single-molecule Force Spectroscopy Reveals the Calcium Dependence of the Alternative Conformations in the Native State of a ??-Crystallin Protein.
Scholl ZN, Li Q, Yang W, Marszalek PE
The Journal of biological chemistry291(35)18263-75(2016 08)

DNMT1 and AIM1 Imprinting in human placenta revealed through a genome-wide screen for allele-specific DNA methylation.
Das R, Lee YK, Strogantsev R, Jin S, Lim YC, Ng PY, Lin XM, Chng K, Yeo GSh, Ferguson-Smith AC, Ding C
BMC genomics14685(2013 Oct)

AIM1 promoter hypermethylation as a predictor of decreased risk of recurrence following radical prostatectomy.
Rosenbaum E, Begum S, Brait M, Zahurak M, Maldonado L, Mangold LA, Eisenberger MA, Epstein JI, Partin AW, Sidransky D, Hoque MO
The Prostate72(10)1133-9(2012 Jul)

AIM1 and LINE-1 epigenetic aberrations in tumor and serum relate to melanoma progression and disease outcome.
Hoshimoto S, Kuo CT, Chong KK, Takeshima TL, Takei Y, Li MW, Huang SK, Sim MS, Morton DL, Hoon DS
The Journal of investigative dermatology132(6)1689-97(2012 Jun)

High-throughput transcriptomic and RNAi analysis identifies AIM1, ERGIC1, TMED3 and TPX2 as potential drug targets in prostate cancer.
Vainio P, Mpindi JP, Kohonen P, Fey V, Mirtti T, Alanen KA, Per?l? M, Kallioniemi O, Iljin K
PloS one7(6)e39801(2012)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Single-molecule Force Spectroscopy Reveals the Calcium Dependence of the Alternative Conformations in the Native State of a betagamma-Crystallin Protein.
Title: Single-molecule Force Spectroscopy Reveals the Calcium Dependence of the Alternative Conformations in the Native State of a ??-Crystallin Protein.

Both DNMT1 and AIM1 show maternal allele-specific methylation and paternal allele-specific transcription.
Title: DNMT1 and AIM1 Imprinting in human placenta revealed through a genome-wide screen for allele-specific DNA methylation.

Methylation status of AIM1 in the prostate cancer specimen may predict for time to recurrence in Gleason 3 + 4 = 7 patients undergoing prostatectomy.
Title: AIM1 promoter hypermethylation as a predictor of decreased risk of recurrence following radical prostatectomy.

AIM1 promoter hypermethylation was found in higher frequency (P = 0.005) in metastatic melanoma (65%) than in primary melanomas (38%).
Title: AIM1 and LINE-1 epigenetic aberrations in tumor and serum relate to melanoma progression and disease outcome.

AIM1, ERGIC1, and TPX2 were shown to be highly expressed especially in prostate cancer tissues, and high mRNA expression of ERGIC1 and TMED3 associated with AR and ERG oncogene expression
Title: High-throughput transcriptomic and RNAi analysis identifies AIM1, ERGIC1, TMED3 and TPX2 as potential drug targets in prostate cancer.

The following CRYBG1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CRYBG1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu56219	NM_001624.4 Latest version!	Homo sapiens crystallin beta-gamma domain containing 1 (CRYBG1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu40642	NM_001371242.2 Latest version!	Homo sapiens crystallin beta-gamma domain containing 1 (CRYBG1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OHu40642	XM_005266839.3 Latest version!	Homo sapiens crystallin beta-gamma domain containing 1 (CRYBG1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1091.30 $1559.00
OHu96335	XM_017010333.1 Latest version!	Homo sapiens crystallin beta-gamma domain containing 1 (CRYBG1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$811.30 $1159.00
View Old Accession Versions >>
OHu40642	NM_001371242.1	Homo sapiens crystallin beta-gamma domain containing 1 (CRYBG1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1091.30 $1559.00
OHu56219	NM_001624.3	Homo sapiens crystallin beta-gamma domain containing 1 (CRYBG1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CRYBG1 ( NM_001624.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_001615.2 Homo sapiens crystallin beta-gamma domain containing 1 (CRYBG1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu56219
Clone ID Related Accession (Same CDS sequence)	NM_001624.4 , NM_001624.3
Accession Version	NM_001624.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5172bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-26
Organism	Homo sapiens(human)
Product	beta/gamma crystallin domain-containing protein 1 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL359292.12 and AL390074.17. On Aug 27, 2019 this sequence version replaced NM_001624.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U83115.1, SRR1803616.11797.1 [ECO:0000332] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGAAGA GGTCCAGCGG CCGTAGGTCG GGGAGGCGGA GGGGGTCGCA GAAATCCACC 
GACTCCCCCG GCGCGGACGC CGAGCTCCCT GAGAGCGCTG CCAGGGACGA CGCGGTGTTC 
GACGACGAGG TGGCGCCAAA CGCGGCCAGC GATAACGCCT CGGCGGAAAA GAAAGTGAAA 
TCTCCGCGGG CAGCCCTCGA CGGGGGCGTT GCCTCCGCTG CGAGCCCAGA GTCCAAGCCC 
AGCCCCGGTA CCAAAGGGCA GCTCCGAGGG GAGTCGGACC GGAGCAAACA GCCACCCCCG 
GCTTCGTCCC CCACGAAGAG GAAGGGCAGG AGCCGTGCCC TCGAGGCCGT GCCCGCCCCG 
CCCGCCAGCG GCCCCCGGGC TCCCGCCAAG GAGTCCCCAC CCAAGAGGGT GCCCGATCCC 
AGCCCAGTCA CCAAGGGCAC TGCGGCCGAG AGCGGGGAGG AGGCGGCGCG GGCCATCCCC 
CGCGAGCTCC CGGTCAAGAG CAGCTCGCTG CTGCCGGAGA TCAAGCCCGA GCACAAGAGG 
GGCCCGCTCC CCAACCACTT CAACGGCCGG GCAGAGGGAG GTCGAAGCAG AGAGCTGGGC 
AGAGCGGCCG GAGCGCCTGG AGCTTCTGAC GCCGACGGCT TGAAGCCCAG GAACCATTTC 
GGCGTGGGCA GGTCGACAGT GACCACTAAA GTGACCCTCC CTGCCAAGCC CAAACATGTG 
GAACTAAATC TTAAAACCCC TAAGAATCTT GACAGTTTGG GAAATGAGCA CAATCCATTT 
AGCCAGCCAG TTCACAAAGG CAACACTGCC ACCAAAATCT CCTTATTTGA AAACAAACGG 
ACAAACAGTA GCCCAAGACA CACTGACATT CGAGGCCAAA GGAATACTCC TGCCTCTAGT 
AAAACGTTTG TTGGGAGGGC AAAGCTGAAT TTAGCCAAAA AAGCCAAAGA AATGGAGCAA 
CCTGAAAAGA AAGTAATGCC AAACAGTCCC CAGAATGGTG TGCTGGTTAA GGAAACTGCT 
ATAGAAACCA AAGTTACCGT CTCGGAAGAA GAGATTCTGC CAGCAACCAG AGGAATGAAT 
GGAGACTCTT CTGAGAATCA AGCTCTTGGT CCTCAGCCTA ACCAAGATGA TAAAGCAGAT 
GTACAAACAG ATGCTGGCTG CCTTTCAGAA CCAGTGGCTT CTGCTCTGAT TCCTGTCAAG 
GATCATAAGC TCTTAGAGAA GGAGGACTCA GAGGCTGCAG ACAGCAAAAG CCTTGTACTT 
GAAAATGTAA CCGATACAGC ACAAGACATC CCCACCACTG TGGATACCAA AGATTTACCT 
CCAACGGCCA TGCCAAAGCC ACAGCATACA TTTTCTGACT CACAGTCCCC TGCTGAGTCA 
TCTCCTGGGC CTTCTCTTTC ACTGTCTGCA CCCGCTCCTG GGGATGTTCC CAAAGACACA 
TGTGTTCAAT CACCCATAAG CAGTTTCCCA TGCACTGATC TAAAAGTGTC AGAAAACCAT 
AAAGGATGTG TTTTGCCTGT GTCTCGTCAG AACAATGAGA AAATGCCACT TTTAGAACTT 
GGAGGAGAAA CAACCCCTCC TTTGTCCACA GAGCGTAGTC CAGAAGCTGT GGGAAGTGAG 
TGTCCATCCA GAGTCCTCGT CCAGGTCAGG TCCTTCGTGC TCCCCGTGGA GAGCACCCAG 
GATGTGAGCT CCCAGGTCAT CCCAGAGAGC TCTGAAGTTA GAGAAGTGCA GTTGCCAACT 
TGTCACAGTA ATGAACCTGA AGTGGTTTCC GTTGCAAGTT GTGCTCCCCC ACAAGAGGAA 
GTACTGGGCA ATGAACACTC TCATTGCACA GCAGAGCTCG CGGCAAAATC TGGCCCACAA 
GTCATACCGC CAGCATCAGA GAAAACTCTG CCTATTCAGG CTCAAAGTCA GGGCAGCAGA 
ACACCCCTGA TGGCTGAATC CAGTCCCACC AACTCTCCCA GCAGCGGAAA TCACTTAGCC 
ACTCCTCAAA GGCCAGATCA GACTGTTACA AATGGCCAGG ATAGCCCTGC CAGCCTTTTG 
AACATTTCTG CTGGTAGTGA TGATAGTGTA TTTGATTCTT CTTCTGATAT GGAAAAATTC 
ACTGAAATTA TAAAACAGAT GGATAGCGCA GTTTGTATGC CCATGAAAAG AAAGAAGGCC 
AGGATGCCAA ACTCTCCTGC TCCTCACTTT GCCATGCCTC CTATTCACGA AGACCATTTA 
GAAAAGGTGT TTGATCCCAA AGTGTTTACC TTTGGTTTGG GGAAGAAGAA GGAAAGTCAG 
CCAGAAATGT CACCGGCTTT ACATTTGATG CAGAACCTTG ACACAAAATC CAAACTGAGA 
CCCAAACGTG CATCTGCTGA ACAGAGCGTC CTCTTCAAGT CCCTGCACAC CAACACTAAT 
GGGAACAGTG AGCCTCTGGT GATGCCGGAA ATCAATGACA AAGAGAACAG GGACGTCACA 
AATGGTGGCA TTAAGAGATC GAGACTAGAA AAAAGTGCAC TTTTCTCAAG CTTGTTATCT 
TCTTTACCAC AAGACAAAAT CTTTTCTCCT TCTGTGACAT CAGTCAACAC TATGACCACG 
GCTTTCAGTA CTTCTCAGAA CGGTTCCCTA TCTCAGTCTT CAGTGTCACA GCCCACGACT 
GAGGGTGCCC CGCCCTGTGG TTTGAACAAA GAACAGTCAA ATCTTCTGCC CGACAACTCC 
TTAAAGGTCT TCAATTTCAA CTCGTCAAGT ACATCACACT CCAGTTTGAA AAGTCCAAGC 
CACATGGAAA AATACCCGCA AAAAGAGAAA ACCAAAGAAG ATCTGGATTC ACGAAGCAAC 
CTACACTTGC CAGAAACTAA ATTTTCTGAA TTGTCAAAAC TGAAGAATGA TGATATGGAA 
AAGGCTAATC ATATTGAAAG TGTTATTAAA TCAAACTTGC CAAACTGTGC AAACAGTGAC 
ACCGACTTCA TGGGTCTTTT CAAATCAAGC CGGTATGACC CAAGCATTTC TTTTTCTGGA 
ATGTCATTAT CAGACACAAT GACACTTAGA GGAAGTGTCC AAAATAAACT CAATCCCCGA 
CCTGGAAAGG TAGTGATATA TAGTGAACCC GACGTCTCTG AGAAGTGCAT TGAAGTTTTC 
AGTGACATTC AGGATTGCAG TTCTTGGAGC CTCTCTCCAG TGATACTCAT AAAAGTTGTT 
AGAGGATGTT GGATTTTGTA TGAGCAACCA AATTTTGAAG GGCACTCCAT CCCCTTAGAA 
GAAGGAGAAT TGGAACTCTC TGGTCTCTGG GGTATAGAAG ACATTTTGGA AAGGCACGAA 
GAAGCAGAGT CTGATAAGCC AGTGGTGATT GGTTCCATCA GACATGTGGT TCAGGATTAC 
AGAGTTAGTC ACATTGACTT ATTTACTGAA CCAGAAGGGT TAGGAATCCT AAGTTCCTAC 
TTTGATGATA CTGAAGAAAT GCAGGGATTT GGTGTAATGC AGAAGACTTG TTCCATGAAA 
GTACATTGGG GCACGTGGCT GATTTATGAA GAACCTGGAT TTCAGGGTGT TCCTTTCATC 
CTGGAACCTG GTGAATACCC TGACTTGTCC TTCTGGGATA CAGAAGAAGC GTACATTGGA 
TCCATGCGGC CTCTGAAAAT GGGTGGCCGT AAAGTTGAAT TCCCTACAGA TCCAAAGGTA 
GTTGTTTATG AAAAGCCTTT CTTTGAAGGA AAATGTGTGG AACTAGAAAC AGGAATGTGT 
AGTTTTGTCA TGGAGGGAGG TGAAACAGAA GAGGCGACTG GAGACGATCA TTTGCCGTTT 
ACGTCAGTGG GGTCTATGAA AGTTCTAAGA GGCATTTGGG TTGCATATGA GAAACCTGGA 
TTTACCGGTC ATCAGTATTT GCTAGAAGAA GGAGAATACA GGGACTGGAA AGCCTGGGGA 
GGTTACAATG GAGAGCTTCA GTCTTTACGA CCTATATTAG GTGATTTTTC AAATGCTCAC 
ATGATAATGT ACAGTGAAAA AAACTTTGGA TCCAAAGGTT CCAGTATTGA TGTATTGGGA 
ATTGTTGCTA ATTTAAAGGA GACTGGATAT GGAGTGAAGA CACAGTCTAT TAATGTACTG 
AGTGGAGTAT GGGTAGCCTA TGAAAATCCT GACTTCACAG GAGAACAGTA TATACTGGAT 
AAAGGATTTT ATACCAGTTT TGAGGACTGG GGAGGCAAAA ATTGTAAGAT CTCTTCTGTT 
CAACCTATAT GTTTGGATTC TTTCACTGGC CCAAGGAGAC GAAATCAGAT TCACTTGTTT 
TCAGAACCAC AGTTTCAAGG TCACAGTCAA AGTTTTGAAG AAACAACAAG TCAAATTGAT 
GATTCATTTT CTACCAAGTC TTGCAGAGTT TCAGGAGGCA GCTGGGTTGT ATATGATGGA 
GAAAATTTCA CTGGTAATCA ATACGTGTTG GAAGAAGGCC ATTATCCTTG TCTGTCTGCA 
ATGGGATGCC CGCCTGGAGC AACTTTCAAG TCTCTTCGTT TTATAGATGT TGAATTTTCT 
GAACCAACAA TTATTCTCTT TGAAAGAGAA GACTTCAAAG GAAAAAAGAT TGAACTTAAT 
GCAGAAACTG TCAATCTCCG ATCCCTGGGA TTCAACACAC AAATACGCTC TGTTCAGGTT 
ATTGGTGGCA TATGGGTTAC TTATGAATAT GGCAGTTACA GAGGGCGACA GTTCCTATTG 
TCACCTGCAG AAGTACCTAA TTGGTATGAA TTCAGTGGCT GTCGCCAAAT AGGTTCTCTA 
CGACCTTTTG TTCAGAAGCG AATTTATTTC AGACTTCGAA ACAAAGCAAC AGGGTTATTC 
ATGTCAACCA ATGGAAACTT AGAGGATCTG AAGCTTCTGA GGATACAGGT CATGGAGGAT 
GTCGGGGCCG ATGATCAGAT TTGGATCTAT CAAGAAGGAT GTATCAAATG CAGGATAGCA 
GAAGACTGCT GCCTGACGAT TGTGGGCAGC CTGGTAACAT CTGGCTCCAA GCTAGGCCTG 
GCCCTGGACC AGAATGCTGA CAGCCAGTTC TGGAGCTTGA AGTCCGATGG CAGGATTTAC 
AGCAAGTTGA AGCCAAATTT AGTTTTAGAC ATTAAAGGGG GCACACAGTA TGATCAAAAT 
CACATTATCC TCAACACTGT CAGCAAAGAG AAGTTTACAC AAGTGTGGGA AGCCATGGTC 
CTATATACCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001615.2
CDS	488..5659
Translation	MEKRSSGRRSGRRRGSQKSTDSPGADAELPESAARDDAVFDDEVAPNAASDNASAEKKVKSPRAALDGGVASAASPESKPSPGTKGQLRGESDRSKQPPPASSPTKRKGRSRALEAVPAPPASGPRAPAKESPPKRVPDPSPVTKGTAAESGEEAARAIPRELPVKSSSLLPEIKPEHKRGPLPNHFNGRAEGGRSRELGRAAGAPGASDADGLKPRNHFGVGRSTVTTKVTLPAKPKHVELNLKTPKNLDSLGNEHNPFSQPVHKGNTATKISLFENKRTNSSPRHTDIRGQRNTPASSKTFVGRAKLNLAKKAKEMEQPEKKVMPNSPQNGVLVKETAIETKVTVSEEEILPATRGMNGDSSENQALGPQPNQDDKADVQTDAGCLSEPVASALIPVKDHKLLEKEDSEAADSKSLVLENVTDTAQDIPTTVDTKDLPPTAMPKPQHTFSDSQSPAESSPGPSLSLSAPAPGDVPKDTCVQSPISSFPCTDLKVSENHKGCVLPVSRQNNEKMPLLELGGETTPPLSTERSPEAVGSECPSRVLVQVRSFVLPVESTQDVSSQVIPESSEVREVQLPTCHSNEPEVVSVASCAPPQEEVLGNEHSHCTAELAAKSGPQVIPPASEKTLPIQAQSQGSRTPLMAESSPTNSPSSGNHLATPQRPDQTVTNGQDSPASLLNISAGSDDSVFDSSSDMEKFTEIIKQMDSAVCMPMKRKKARMPNSPAPHFAMPPIHEDHLEKVFDPKVFTFGLGKKKESQPEMSPALHLMQNLDTKSKLRPKRASAEQSVLFKSLHTNTNGNSEPLVMPEINDKENRDVTNGGIKRSRLEKSALFSSLLSSLPQDKIFSPSVTSVNTMTTAFSTSQNGSLSQSSVSQPTTEGAPPCGLNKEQSNLLPDNSLKVFNFNSSSTSHSSLKSPSHMEKYPQKEKTKEDLDSRSNLHLPETKFSELSKLKNDDMEKANHIESVIKSNLPNCANSDTDFMGLFKSSRYDPSISFSGMSLSDTMTLRGSVQNKLNPRPGKVVIYSEPDVSEKCIEVFSDIQDCSSWSLSPVILIKVVRGCWILYEQPNFEGHSIPLEEGELELSGLWGIEDILERHEEAESDKPVVIGSIRHVVQDYRVSHIDLFTEPEGLGILSSYFDDTEEMQGFGVMQKTCSMKVHWGTWLIYEEPGFQGVPFILEPGEYPDLSFWDTEEAYIGSMRPLKMGGRKVEFPTDPKVVVYEKPFFEGKCVELETGMCSFVMEGGETEEATGDDHLPFTSVGSMKVLRGIWVAYEKPGFTGHQYLLEEGEYRDWKAWGGYNGELQSLRPILGDFSNAHMIMYSEKNFGSKGSSIDVLGIVANLKETGYGVKTQSINVLSGVWVAYENPDFTGEQYILDKGFYTSFEDWGGKNCKISSVQPICLDSFTGPRRRNQIHLFSEPQFQGHSQSFEETTSQIDDSFSTKSCRVSGGSWVVYDGENFTGNQYVLEEGHYPCLSAMGCPPGATFKSLRFIDVEFSEPTIILFEREDFKGKKIELNAETVNLRSLGFNTQIRSVQVIGGIWVTYEYGSYRGRQFLLSPAEVPNWYEFSGCRQIGSLRPFVQKRIYFRLRNKATGLFMSTNGNLEDLKLLRIQVMEDVGADDQIWIYQEGCIKCRIAEDCCLTIVGSLVTSGSKLGLALDQNADSQFWSLKSDGRIYSKLKPNLVLDIKGGTQYDQNHIILNTVSKEKFTQVWEAMVLYT

Target ORF information:

RefSeq Version	NM_001624.4
Organism	Homo sapiens(human)
Definition	Homo sapiens crystallin beta-gamma domain containing 1 (CRYBG1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001624.4

ORF Insert Sequence:

ATGGAGAAGA GGTCCAGCGG CCGTAGGTCG GGGAGGCGGA GGGGGTCGCA GAAATCCACC 
GACTCCCCCG GCGCGGACGC CGAGCTCCCT GAGAGCGCTG CCAGGGACGA CGCGGTGTTC 
GACGACGAGG TGGCGCCAAA CGCGGCCAGC GATAACGCCT CGGCGGAAAA GAAAGTGAAA 
TCTCCGCGGG CAGCCCTCGA CGGGGGCGTT GCCTCCGCTG CGAGCCCAGA GTCCAAGCCC 
AGCCCCGGTA CCAAAGGGCA GCTCCGAGGG GAGTCGGACC GGAGCAAACA GCCACCCCCG 
GCTTCGTCCC CCACGAAGAG GAAGGGCAGG AGCCGTGCCC TCGAGGCCGT GCCCGCCCCG 
CCCGCCAGCG GCCCCCGGGC TCCCGCCAAG GAGTCCCCAC CCAAGAGGGT GCCCGATCCC 
AGCCCAGTCA CCAAGGGCAC TGCGGCCGAG AGCGGGGAGG AGGCGGCGCG GGCCATCCCC 
CGCGAGCTCC CGGTCAAGAG CAGCTCGCTG CTGCCGGAGA TCAAGCCCGA GCACAAGAGG 
GGCCCGCTCC CCAACCACTT CAACGGCCGG GCAGAGGGAG GTCGAAGCAG AGAGCTGGGC 
AGAGCGGCCG GAGCGCCTGG AGCTTCTGAC GCCGACGGCT TGAAGCCCAG GAACCATTTC 
GGCGTGGGCA GGTCGACAGT GACCACTAAA GTGACCCTCC CTGCCAAGCC CAAACATGTG 
GAACTAAATC TTAAAACCCC TAAGAATCTT GACAGTTTGG GAAATGAGCA CAATCCATTT 
AGCCAGCCAG TTCACAAAGG CAACACTGCC ACCAAAATCT CCTTATTTGA AAACAAACGG 
ACAAACAGTA GCCCAAGACA CACTGACATT CGAGGCCAAA GGAATACTCC TGCCTCTAGT 
AAAACGTTTG TTGGGAGGGC AAAGCTGAAT TTAGCCAAAA AAGCCAAAGA AATGGAGCAA 
CCTGAAAAGA AAGTAATGCC AAACAGTCCC CAGAATGGTG TGCTGGTTAA GGAAACTGCT 
ATAGAAACCA AAGTTACCGT CTCGGAAGAA GAGATTCTGC CAGCAACCAG AGGAATGAAT 
GGAGACTCTT CTGAGAATCA AGCTCTTGGT CCTCAGCCTA ACCAAGATGA TAAAGCAGAT 
GTACAAACAG ATGCTGGCTG CCTTTCAGAA CCAGTGGCTT CTGCTCTGAT TCCTGTCAAG 
GATCATAAGC TCTTAGAGAA GGAGGACTCA GAGGCTGCAG ACAGCAAAAG CCTTGTACTT 
GAAAATGTAA CCGATACAGC ACAAGACATC CCCACCACTG TGGATACCAA AGATTTACCT 
CCAACGGCCA TGCCAAAGCC ACAGCATACA TTTTCTGACT CACAGTCCCC TGCTGAGTCA 
TCTCCTGGGC CTTCTCTTTC ACTGTCTGCA CCCGCTCCTG GGGATGTTCC CAAAGACACA 
TGTGTTCAAT CACCCATAAG CAGTTTCCCA TGCACTGATC TAAAAGTGTC AGAAAACCAT 
AAAGGATGTG TTTTGCCTGT GTCTCGTCAG AACAATGAGA AAATGCCACT TTTAGAACTT 
GGAGGAGAAA CAACCCCTCC TTTGTCCACA GAGCGTAGTC CAGAAGCTGT GGGAAGTGAG 
TGTCCATCCA GAGTCCTCGT CCAGGTCAGG TCCTTCGTGC TCCCCGTGGA GAGCACCCAG 
GATGTGAGCT CCCAGGTCAT CCCAGAGAGC TCTGAAGTTA GAGAAGTGCA GTTGCCAACT 
TGTCACAGTA ATGAACCTGA AGTGGTTTCC GTTGCAAGTT GTGCTCCCCC ACAAGAGGAA 
GTACTGGGCA ATGAACACTC TCATTGCACA GCAGAGCTCG CGGCAAAATC TGGCCCACAA 
GTCATACCGC CAGCATCAGA GAAAACTCTG CCTATTCAGG CTCAAAGTCA GGGCAGCAGA 
ACACCCCTGA TGGCTGAATC CAGTCCCACC AACTCTCCCA GCAGCGGAAA TCACTTAGCC 
ACTCCTCAAA GGCCAGATCA GACTGTTACA AATGGCCAGG ATAGCCCTGC CAGCCTTTTG 
AACATTTCTG CTGGTAGTGA TGATAGTGTA TTTGATTCTT CTTCTGATAT GGAAAAATTC 
ACTGAAATTA TAAAACAGAT GGATAGCGCA GTTTGTATGC CCATGAAAAG AAAGAAGGCC 
AGGATGCCAA ACTCTCCTGC TCCTCACTTT GCCATGCCTC CTATTCACGA AGACCATTTA 
GAAAAGGTGT TTGATCCCAA AGTGTTTACC TTTGGTTTGG GGAAGAAGAA GGAAAGTCAG 
CCAGAAATGT CACCGGCTTT ACATTTGATG CAGAACCTTG ACACAAAATC CAAACTGAGA 
CCCAAACGTG CATCTGCTGA ACAGAGCGTC CTCTTCAAGT CCCTGCACAC CAACACTAAT 
GGGAACAGTG AGCCTCTGGT GATGCCGGAA ATCAATGACA AAGAGAACAG GGACGTCACA 
AATGGTGGCA TTAAGAGATC GAGACTAGAA AAAAGTGCAC TTTTCTCAAG CTTGTTATCT 
TCTTTACCAC AAGACAAAAT CTTTTCTCCT TCTGTGACAT CAGTCAACAC TATGACCACG 
GCTTTCAGTA CTTCTCAGAA CGGTTCCCTA TCTCAGTCTT CAGTGTCACA GCCCACGACT 
GAGGGTGCCC CGCCCTGTGG TTTGAACAAA GAACAGTCAA ATCTTCTGCC CGACAACTCC 
TTAAAGGTCT TCAATTTCAA CTCGTCAAGT ACATCACACT CCAGTTTGAA AAGTCCAAGC 
CACATGGAAA AATACCCGCA AAAAGAGAAA ACCAAAGAAG ATCTGGATTC ACGAAGCAAC 
CTACACTTGC CAGAAACTAA ATTTTCTGAA TTGTCAAAAC TGAAGAATGA TGATATGGAA 
AAGGCTAATC ATATTGAAAG TGTTATTAAA TCAAACTTGC CAAACTGTGC AAACAGTGAC 
ACCGACTTCA TGGGTCTTTT CAAATCAAGC CGGTATGACC CAAGCATTTC TTTTTCTGGA 
ATGTCATTAT CAGACACAAT GACACTTAGA GGAAGTGTCC AAAATAAACT CAATCCCCGA 
CCTGGAAAGG TAGTGATATA TAGTGAACCC GACGTCTCTG AGAAGTGCAT TGAAGTTTTC 
AGTGACATTC AGGATTGCAG TTCTTGGAGC CTCTCTCCAG TGATACTCAT AAAAGTTGTT 
AGAGGATGTT GGATTTTGTA TGAGCAACCA AATTTTGAAG GGCACTCCAT CCCCTTAGAA 
GAAGGAGAAT TGGAACTCTC TGGTCTCTGG GGTATAGAAG ACATTTTGGA AAGGCACGAA 
GAAGCAGAGT CTGATAAGCC AGTGGTGATT GGTTCCATCA GACATGTGGT TCAGGATTAC 
AGAGTTAGTC ACATTGACTT ATTTACTGAA CCAGAAGGGT TAGGAATCCT AAGTTCCTAC 
TTTGATGATA CTGAAGAAAT GCAGGGATTT GGTGTAATGC AGAAGACTTG TTCCATGAAA 
GTACATTGGG GCACGTGGCT GATTTATGAA GAACCTGGAT TTCAGGGTGT TCCTTTCATC 
CTGGAACCTG GTGAATACCC TGACTTGTCC TTCTGGGATA CAGAAGAAGC GTACATTGGA 
TCCATGCGGC CTCTGAAAAT GGGTGGCCGT AAAGTTGAAT TCCCTACAGA TCCAAAGGTA 
GTTGTTTATG AAAAGCCTTT CTTTGAAGGA AAATGTGTGG AACTAGAAAC AGGAATGTGT 
AGTTTTGTCA TGGAGGGAGG TGAAACAGAA GAGGCGACTG GAGACGATCA TTTGCCGTTT 
ACGTCAGTGG GGTCTATGAA AGTTCTAAGA GGCATTTGGG TTGCATATGA GAAACCTGGA 
TTTACCGGTC ATCAGTATTT GCTAGAAGAA GGAGAATACA GGGACTGGAA AGCCTGGGGA 
GGTTACAATG GAGAGCTTCA GTCTTTACGA CCTATATTAG GTGATTTTTC AAATGCTCAC 
ATGATAATGT ACAGTGAAAA AAACTTTGGA TCCAAAGGTT CCAGTATTGA TGTATTGGGA 
ATTGTTGCTA ATTTAAAGGA GACTGGATAT GGAGTGAAGA CACAGTCTAT TAATGTACTG 
AGTGGAGTAT GGGTAGCCTA TGAAAATCCT GACTTCACAG GAGAACAGTA TATACTGGAT 
AAAGGATTTT ATACCAGTTT TGAGGACTGG GGAGGCAAAA ATTGTAAGAT CTCTTCTGTT 
CAACCTATAT GTTTGGATTC TTTCACTGGC CCAAGGAGAC GAAATCAGAT TCACTTGTTT 
TCAGAACCAC AGTTTCAAGG TCACAGTCAA AGTTTTGAAG AAACAACAAG TCAAATTGAT 
GATTCATTTT CTACCAAGTC TTGCAGAGTT TCAGGAGGCA GCTGGGTTGT ATATGATGGA 
GAAAATTTCA CTGGTAATCA ATACGTGTTG GAAGAAGGCC ATTATCCTTG TCTGTCTGCA 
ATGGGATGCC CGCCTGGAGC AACTTTCAAG TCTCTTCGTT TTATAGATGT TGAATTTTCT 
GAACCAACAA TTATTCTCTT TGAAAGAGAA GACTTCAAAG GAAAAAAGAT TGAACTTAAT 
GCAGAAACTG TCAATCTCCG ATCCCTGGGA TTCAACACAC AAATACGCTC TGTTCAGGTT 
ATTGGTGGCA TATGGGTTAC TTATGAATAT GGCAGTTACA GAGGGCGACA GTTCCTATTG 
TCACCTGCAG AAGTACCTAA TTGGTATGAA TTCAGTGGCT GTCGCCAAAT AGGTTCTCTA 
CGACCTTTTG TTCAGAAGCG AATTTATTTC AGACTTCGAA ACAAAGCAAC AGGGTTATTC 
ATGTCAACCA ATGGAAACTT AGAGGATCTG AAGCTTCTGA GGATACAGGT CATGGAGGAT 
GTCGGGGCCG ATGATCAGAT TTGGATCTAT CAAGAAGGAT GTATCAAATG CAGGATAGCA 
GAAGACTGCT GCCTGACGAT TGTGGGCAGC CTGGTAACAT CTGGCTCCAA GCTAGGCCTG 
GCCCTGGACC AGAATGCTGA CAGCCAGTTC TGGAGCTTGA AGTCCGATGG CAGGATTTAC 
AGCAAGTTGA AGCCAAATTT AGTTTTAGAC ATTAAAGGGG GCACACAGTA TGATCAAAAT 
CACATTATCC TCAACACTGT CAGCAAAGAG AAGTTTACAC AAGTGTGGGA AGCCATGGTC 
CTATATACCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CRYBG1 ( NM_001371242.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001358171.1 Homo sapiens crystallin beta-gamma domain containing 1 (CRYBG1), transcript variant 1, mRNA.

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CloneID	OHu40642
Clone ID Related Accession (Same CDS sequence)	NM_001371242.1 , XM_005266839.3 , NM_001371242.2
Accession Version	NM_001371242.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6396bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-29
Organism	Homo sapiens(human)
Product	beta/gamma crystallin domain-containing protein 1 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL109920.15, AL359292.12 and AL390074.17. On Aug 27, 2019 this sequence version replaced NM_001371242.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##RefSeq-Attributes-START## RefSeq Select criteria :: based on manual assertion, conservation, longest protein ##RefSeq-Attributes-END##

ATGCCGCTGT CCCCGCCAGC CCAGGGCGAC CCCGGGGAGC CCAGCCCGTG CAGGCCCCCT 
AAGAAGCACA CCACCTTCCA CCTCTGGCGC TCCAAAAAGA AGCAGCAGCC GGCGCCGCCT 
GACTGTGGGG TGTTCGTTCC GCACCCGCTC CCGGCGCCTG CCGGAGAGGC CAGAGCTTTG 
GATGTAGTCG ATGGAAAATA TGTGGTTCGA GACTCCCAGG AATTTCCACT GCACTGTGGG 
GAATCCCAGT TCTTCCACAC CACCAGTGAG GCGCTTGGTT CCTTACTTCT AGAGTCTGGA 
ATATTTAAAA AGTCCAGAGC ACAACCTCCA GAAGACAACA GAAGGAAGCC AGTTTTGGGG 
AAACTTGGCA CTCTATTCAC TGCAGGAAGG AGAAGAAACA GTAGAAACGG GTTAGAGAGT 
CCCACCAGAT CAAATGCCAA ACCACTCTCT CCCAAAGATG TGGTAGCCTC TCCTAAGCTC 
CCAGAGAGAG AGAGTGAGAG GAGCAGATCT CAGAGCAGCC AACTGAAGCA AACGGACACA 
AGCGAGGAGG GCTCCCCGCG GGAGAATCCC CGAGAGGCAG AGGGCGAGCT CCCCGAGAGC 
GGTGGCCCCG CAGCCCCCCC TGACGCCGAG CTGTCACCTC GCTGGAGCAG CAGTGCAGCG 
GCTGTGGCTG TGCAGCAGTG CCATGAAAAT GATTCACCCC AATTAGAACC TCTGGAGGCA 
GAGGGAGAGC CTTTCCCAGA TGCCACCACC ACTGCCAAGC AGCTGCATTC CTCGCCGGGA 
AATTCCTCCA GGCAAGAGAA CGCAGAGACG CCCGCCCGCA GTCCGGGGGA GGACGCTTCA 
CCAGGTGCTG GCCACGAACA GGAGGCTTTC CTGGGTGTGA GGGGTGCGCC AGGGTCGCCC 
ACCCAGGAGC GGCCCGCGGG AGGACTAGGC GAGGCCCCTA ACGGAGCCCC CAGTGTGTGT 
GCCGAAGAAG GCTCCCTGGG GCCCCGCAAC GCCCGCAGCC AGCCCCCCAA GGGCGCGTCT 
GATTTGCCAG GTGAGCCTCC GGCCGAGGGC GCAGCGCACA CGGCCAGCTC CGCGCAGGCA 
GACTGCACAG CCCGCCCCAA GGGTCACGCC CACCCTGCTA AGGTGCTAAC TTTGGACATC 
TACTTGAGTA AGACTGAGGG GGCACAAGTG GACGAGCCGG TCGTGATTAC TCCCAGAGCG 
GAAGATTGCG GTGACTGGGA CGACATGGAG AAGAGGTCCA GCGGCCGTAG GTCGGGGAGG 
CGGAGGGGGT CGCAGAAATC CACCGACTCC CCCGGCGCGG ACGCCGAGCT CCCTGAGAGC 
GCTGCCAGGG ACGACGCGGT GTTCGACGAC GAGGTGGCGC CAAACGCGGC CAGCGATAAC 
GCCTCGGCGG AAAAGAAAGT GAAATCTCCG CGGGCAGCCC TCGACGGGGG CGTTGCCTCC 
GCTGCGAGCC CAGAGTCCAA GCCCAGCCCC GGTACCAAAG GGCAGCTCCG AGGGGAGTCG 
GACCGGAGCA AACAGCCACC CCCGGCTTCG TCCCCCACGA AGAGGAAGGG CAGGAGCCGT 
GCCCTCGAGG CCGTGCCCGC CCCGCCCGCC AGCGGCCCCC GGGCTCCCGC CAAGGAGTCC 
CCACCCAAGA GGGTGCCCGA TCCCAGCCCA GTCACCAAGG GCACTGCGGC CGAGAGCGGG 
GAGGAGGCGG CGCGGGCCAT CCCCCGCGAG CTCCCGGTCA AGAGCAGCTC GCTGCTGCCG 
GAGATCAAGC CCGAGCACAA GAGGGGCCCG CTCCCCAACC ACTTCAACGG CCGGGCAGAG 
GGAGGTCGAA GCAGAGAGCT GGGCAGAGCG GCCGGAGCGC CTGGAGCTTC TGACGCCGAC 
GGCTTGAAGC CCAGGAACCA TTTCGGCGTG GGCAGGTCGA CAGTGACCAC TAAAGTGACC 
CTCCCTGCCA AGCCCAAACA TGTGGAACTA AATCTTAAAA CCCCTAAGAA TCTTGACAGT 
TTGGGAAATG AGCACAATCC ATTTAGCCAG CCAGTTCACA AAGGCAACAC TGCCACCAAA 
ATCTCCTTAT TTGAAAACAA ACGGACAAAC AGTAGCCCAA GACACACTGA CATTCGAGGC 
CAAAGGAATA CTCCTGCCTC TAGTAAAACG TTTGTTGGGA GGGCAAAGCT GAATTTAGCC 
AAAAAAGCCA AAGAAATGGA GCAACCTGAA AAGAAAGTAA TGCCAAACAG TCCCCAGAAT 
GGTGTGCTGG TTAAGGAAAC TGCTATAGAA ACCAAAGTTA CCGTCTCGGA AGAAGAGATT 
CTGCCAGCAA CCAGAGGAAT GAATGGAGAC TCTTCTGAGA ATCAAGCTCT TGGTCCTCAG 
CCTAACCAAG ATGATAAAGC AGATGTACAA ACAGATGCTG GCTGCCTTTC AGAACCAGTG 
GCTTCTGCTC TGATTCCTGT CAAGGATCAT AAGCTCTTAG AGAAGGAGGA CTCAGAGGCT 
GCAGACAGCA AAAGCCTTGT ACTTGAAAAT GTAACCGATA CAGCACAAGA CATCCCCACC 
ACTGTGGATA CCAAAGATTT ACCTCCAACG GCCATGCCAA AGCCACAGCA TACATTTTCT 
GACTCACAGT CCCCTGCTGA GTCATCTCCT GGGCCTTCTC TTTCACTGTC TGCACCCGCT 
CCTGGGGATG TTCCCAAAGA CACATGTGTT CAATCACCCA TAAGCAGTTT CCCATGCACT 
GATCTAAAAG TGTCAGAAAA CCATAAAGGA TGTGTTTTGC CTGTGTCTCG TCAGAACAAT 
GAGAAAATGC CACTTTTAGA ACTTGGAGGA GAAACAACCC CTCCTTTGTC CACAGAGCGT 
AGTCCAGAAG CTGTGGGAAG TGAGTGTCCA TCCAGAGTCC TCGTCCAGGT CAGGTCCTTC 
GTGCTCCCCG TGGAGAGCAC CCAGGATGTG AGCTCCCAGG TCATCCCAGA GAGCTCTGAA 
GTTAGAGAAG TGCAGTTGCC AACTTGTCAC AGTAATGAAC CTGAAGTGGT TTCCGTTGCA 
AGTTGTGCTC CCCCACAAGA GGAAGTACTG GGCAATGAAC ACTCTCATTG CACAGCAGAG 
CTCGCGGCAA AATCTGGCCC ACAAGTCATA CCGCCAGCAT CAGAGAAAAC TCTGCCTATT 
CAGGCTCAAA GTCAGGGCAG CAGAACACCC CTGATGGCTG AATCCAGTCC CACCAACTCT 
CCCAGCAGCG GAAATCACTT AGCCACTCCT CAAAGGCCAG ATCAGACTGT TACAAATGGC 
CAGGATAGCC CTGCCAGCCT TTTGAACATT TCTGCTGGTA GTGATGATAG TGTATTTGAT 
TCTTCTTCTG ATATGGAAAA ATTCACTGAA ATTATAAAAC AGATGGATAG CGCAGTTTGT 
ATGCCCATGA AAAGAAAGAA GGCCAGGATG CCAAACTCTC CTGCTCCTCA CTTTGCCATG 
CCTCCTATTC ACGAAGACCA TTTAGAAAAG GTGTTTGATC CCAAAGTGTT TACCTTTGGT 
TTGGGGAAGA AGAAGGAAAG TCAGCCAGAA ATGTCACCGG CTTTACATTT GATGCAGAAC 
CTTGACACAA AATCCAAACT GAGACCCAAA CGTGCATCTG CTGAACAGAG CGTCCTCTTC 
AAGTCCCTGC ACACCAACAC TAATGGGAAC AGTGAGCCTC TGGTGATGCC GGAAATCAAT 
GACAAAGAGA ACAGGGACGT CACAAATGGT GGCATTAAGA GATCGAGACT AGAAAAAAGT 
GCACTTTTCT CAAGCTTGTT ATCTTCTTTA CCACAAGACA AAATCTTTTC TCCTTCTGTG 
ACATCAGTCA ACACTATGAC CACGGCTTTC AGTACTTCTC AGAACGGTTC CCTATCTCAG 
TCTTCAGTGT CACAGCCCAC GACTGAGGGT GCCCCGCCCT GTGGTTTGAA CAAAGAACAG 
TCAAATCTTC TGCCCGACAA CTCCTTAAAG GTCTTCAATT TCAACTCGTC AAGTACATCA 
CACTCCAGTT TGAAAAGTCC AAGCCACATG GAAAAATACC CGCAAAAAGA GAAAACCAAA 
GAAGATCTGG ATTCACGAAG CAACCTACAC TTGCCAGAAA CTAAATTTTC TGAATTGTCA 
AAACTGAAGA ATGATGATAT GGAAAAGGCT AATCATATTG AAAGTGTTAT TAAATCAAAC 
TTGCCAAACT GTGCAAACAG TGACACCGAC TTCATGGGTC TTTTCAAATC AAGCCGGTAT 
GACCCAAGCA TTTCTTTTTC TGGAATGTCA TTATCAGACA CAATGACACT TAGAGGAAGT 
GTCCAAAATA AACTCAATCC CCGACCTGGA AAGGTAGTGA TATATAGTGA ACCCGACGTC 
TCTGAGAAGT GCATTGAAGT TTTCAGTGAC ATTCAGGATT GCAGTTCTTG GAGCCTCTCT 
CCAGTGATAC TCATAAAAGT TGTTAGAGGA TGTTGGATTT TGTATGAGCA ACCAAATTTT 
GAAGGGCACT CCATCCCCTT AGAAGAAGGA GAATTGGAAC TCTCTGGTCT CTGGGGTATA 
GAAGACATTT TGGAAAGGCA CGAAGAAGCA GAGTCTGATA AGCCAGTGGT GATTGGTTCC 
ATCAGACATG TGGTTCAGGA TTACAGAGTT AGTCACATTG ACTTATTTAC TGAACCAGAA 
GGGTTAGGAA TCCTAAGTTC CTACTTTGAT GATACTGAAG AAATGCAGGG ATTTGGTGTA 
ATGCAGAAGA CTTGTTCCAT GAAAGTACAT TGGGGCACGT GGCTGATTTA TGAAGAACCT 
GGATTTCAGG GTGTTCCTTT CATCCTGGAA CCTGGTGAAT ACCCTGACTT GTCCTTCTGG 
GATACAGAAG AAGCGTACAT TGGATCCATG CGGCCTCTGA AAATGGGTGG CCGTAAAGTT 
GAATTCCCTA CAGATCCAAA GGTAGTTGTT TATGAAAAGC CTTTCTTTGA AGGAAAATGT 
GTGGAACTAG AAACAGGAAT GTGTAGTTTT GTCATGGAGG GAGGTGAAAC AGAAGAGGCG 
ACTGGAGACG ATCATTTGCC GTTTACGTCA GTGGGGTCTA TGAAAGTTCT AAGAGGCATT 
TGGGTTGCAT ATGAGAAACC TGGATTTACC GGTCATCAGT ATTTGCTAGA AGAAGGAGAA 
TACAGGGACT GGAAAGCCTG GGGAGGTTAC AATGGAGAGC TTCAGTCTTT ACGACCTATA 
TTAGGTGATT TTTCAAATGC TCACATGATA ATGTACAGTG AAAAAAACTT TGGATCCAAA 
GGTTCCAGTA TTGATGTATT GGGAATTGTT GCTAATTTAA AGGAGACTGG ATATGGAGTG 
AAGACACAGT CTATTAATGT ACTGAGTGGA GTATGGGTAG CCTATGAAAA TCCTGACTTC 
ACAGGAGAAC AGTATATACT GGATAAAGGA TTTTATACCA GTTTTGAGGA CTGGGGAGGC 
AAAAATTGTA AGATCTCTTC TGTTCAACCT ATATGTTTGG ATTCTTTCAC TGGCCCAAGG 
AGACGAAATC AGATTCACTT GTTTTCAGAA CCACAGTTTC AAGGTCACAG TCAAAGTTTT 
GAAGAAACAA CAAGTCAAAT TGATGATTCA TTTTCTACCA AGTCTTGCAG AGTTTCAGGA 
GGCAGCTGGG TTGTATATGA TGGAGAAAAT TTCACTGGTA ATCAATACGT GTTGGAAGAA 
GGCCATTATC CTTGTCTGTC TGCAATGGGA TGCCCGCCTG GAGCAACTTT CAAGTCTCTT 
CGTTTTATAG ATGTTGAATT TTCTGAACCA ACAATTATTC TCTTTGAAAG AGAAGACTTC 
AAAGGAAAAA AGATTGAACT TAATGCAGAA ACTGTCAATC TCCGATCCCT GGGATTCAAC 
ACACAAATAC GCTCTGTTCA GGTTATTGGT GGCATATGGG TTACTTATGA ATATGGCAGT 
TACAGAGGGC GACAGTTCCT ATTGTCACCT GCAGAAGTAC CTAATTGGTA TGAATTCAGT 
GGCTGTCGCC AAATAGGTTC TCTACGACCT TTTGTTCAGA AGCGAATTTA TTTCAGACTT 
CGAAACAAAG CAACAGGGTT ATTCATGTCA ACCAATGGAA ACTTAGAGGA TCTGAAGCTT 
CTGAGGATAC AGGTCATGGA GGATGTCGGG GCCGATGATC AGATTTGGAT CTATCAAGAA 
GGATGTATCA AATGCAGGAT AGCAGAAGAC TGCTGCCTGA CGATTGTGGG CAGCCTGGTA 
ACATCTGGCT CCAAGCTAGG CCTGGCCCTG GACCAGAATG CTGACAGCCA GTTCTGGAGC 
TTGAAGTCCG ATGGCAGGAT TTACAGCAAG TTGAAGCCAA ATTTAGTTTT AGACATTAAA 
GGGGGCACAC AGTATGATCA AAATCACATT ATCCTCAACA CTGTCAGCAA AGAGAAGTTT 
ACACAAGTGT GGGAAGCCAT GGTCCTATAT ACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001358171.1
CDS	193..6588
Translation	MPLSPPAQGDPGEPSPCRPPKKHTTFHLWRSKKKQQPAPPDCGVFVPHPLPAPAGEARALDVVDGKYVVRDSQEFPLHCGESQFFHTTSEALGSLLLESGIFKKSRAQPPEDNRRKPVLGKLGTLFTAGRRRNSRNGLESPTRSNAKPLSPKDVVASPKLPERESERSRSQSSQLKQTDTSEEGSPRENPREAEGELPESGGPAAPPDAELSPRWSSSAAAVAVQQCHENDSPQLEPLEAEGEPFPDATTTAKQLHSSPGNSSRQENAETPARSPGEDASPGAGHEQEAFLGVRGAPGSPTQERPAGGLGEAPNGAPSVCAEEGSLGPRNARSQPPKGASDLPGEPPAEGAAHTASSAQADCTARPKGHAHPAKVLTLDIYLSKTEGAQVDEPVVITPRAEDCGDWDDMEKRSSGRRSGRRRGSQKSTDSPGADAELPESAARDDAVFDDEVAPNAASDNASAEKKVKSPRAALDGGVASAASPESKPSPGTKGQLRGESDRSKQPPPASSPTKRKGRSRALEAVPAPPASGPRAPAKESPPKRVPDPSPVTKGTAAESGEEAARAIPRELPVKSSSLLPEIKPEHKRGPLPNHFNGRAEGGRSRELGRAAGAPGASDADGLKPRNHFGVGRSTVTTKVTLPAKPKHVELNLKTPKNLDSLGNEHNPFSQPVHKGNTATKISLFENKRTNSSPRHTDIRGQRNTPASSKTFVGRAKLNLAKKAKEMEQPEKKVMPNSPQNGVLVKETAIETKVTVSEEEILPATRGMNGDSSENQALGPQPNQDDKADVQTDAGCLSEPVASALIPVKDHKLLEKEDSEAADSKSLVLENVTDTAQDIPTTVDTKDLPPTAMPKPQHTFSDSQSPAESSPGPSLSLSAPAPGDVPKDTCVQSPISSFPCTDLKVSENHKGCVLPVSRQNNEKMPLLELGGETTPPLSTERSPEAVGSECPSRVLVQVRSFVLPVESTQDVSSQVIPESSEVREVQLPTCHSNEPEVVSVASCAPPQEEVLGNEHSHCTAELAAKSGPQVIPPASEKTLPIQAQSQGSRTPLMAESSPTNSPSSGNHLATPQRPDQTVTNGQDSPASLLNISAGSDDSVFDSSSDMEKFTEIIKQMDSAVCMPMKRKKARMPNSPAPHFAMPPIHEDHLEKVFDPKVFTFGLGKKKESQPEMSPALHLMQNLDTKSKLRPKRASAEQSVLFKSLHTNTNGNSEPLVMPEINDKENRDVTNGGIKRSRLEKSALFSSLLSSLPQDKIFSPSVTSVNTMTTAFSTSQNGSLSQSSVSQPTTEGAPPCGLNKEQSNLLPDNSLKVFNFNSSSTSHSSLKSPSHMEKYPQKEKTKEDLDSRSNLHLPETKFSELSKLKNDDMEKANHIESVIKSNLPNCANSDTDFMGLFKSSRYDPSISFSGMSLSDTMTLRGSVQNKLNPRPGKVVIYSEPDVSEKCIEVFSDIQDCSSWSLSPVILIKVVRGCWILYEQPNFEGHSIPLEEGELELSGLWGIEDILERHEEAESDKPVVIGSIRHVVQDYRVSHIDLFTEPEGLGILSSYFDDTEEMQGFGVMQKTCSMKVHWGTWLIYEEPGFQGVPFILEPGEYPDLSFWDTEEAYIGSMRPLKMGGRKVEFPTDPKVVVYEKPFFEGKCVELETGMCSFVMEGGETEEATGDDHLPFTSVGSMKVLRGIWVAYEKPGFTGHQYLLEEGEYRDWKAWGGYNGELQSLRPILGDFSNAHMIMYSEKNFGSKGSSIDVLGIVANLKETGYGVKTQSINVLSGVWVAYENPDFTGEQYILDKGFYTSFEDWGGKNCKISSVQPICLDSFTGPRRRNQIHLFSEPQFQGHSQSFEETTSQIDDSFSTKSCRVSGGSWVVYDGENFTGNQYVLEEGHYPCLSAMGCPPGATFKSLRFIDVEFSEPTIILFEREDFKGKKIELNAETVNLRSLGFNTQIRSVQVIGGIWVTYEYGSYRGRQFLLSPAEVPNWYEFSGCRQIGSLRPFVQKRIYFRLRNKATGLFMSTNGNLEDLKLLRIQVMEDVGADDQIWIYQEGCIKCRIAEDCCLTIVGSLVTSGSKLGLALDQNADSQFWSLKSDGRIYSKLKPNLVLDIKGGTQYDQNHIILNTVSKEKFTQVWEAMVLYT

Target ORF information:

RefSeq Version	NM_001371242.2
Organism	Homo sapiens(human)
Definition	Homo sapiens crystallin beta-gamma domain containing 1 (CRYBG1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001371242.2

ORF Insert Sequence:

ATGCCGCTGT CCCCGCCAGC CCAGGGCGAC CCCGGGGAGC CCAGCCCGTG CAGGCCCCCT 
AAGAAGCACA CCACCTTCCA CCTCTGGCGC TCCAAAAAGA AGCAGCAGCC GGCGCCGCCT 
GACTGTGGGG TGTTCGTTCC GCACCCGCTC CCGGCGCCTG CCGGAGAGGC CAGAGCTTTG 
GATGTAGTCG ATGGAAAATA TGTGGTTCGA GACTCCCAGG AATTTCCACT GCACTGTGGG 
GAATCCCAGT TCTTCCACAC CACCAGTGAG GCGCTTGGTT CCTTACTTCT AGAGTCTGGA 
ATATTTAAAA AGTCCAGAGC ACAACCTCCA GAAGACAACA GAAGGAAGCC AGTTTTGGGG 
AAACTTGGCA CTCTATTCAC TGCAGGAAGG AGAAGAAACA GTAGAAACGG GTTAGAGAGT 
CCCACCAGAT CAAATGCCAA ACCACTCTCT CCCAAAGATG TGGTAGCCTC TCCTAAGCTC 
CCAGAGAGAG AGAGTGAGAG GAGCAGATCT CAGAGCAGCC AACTGAAGCA AACGGACACA 
AGCGAGGAGG GCTCCCCGCG GGAGAATCCC CGAGAGGCAG AGGGCGAGCT CCCCGAGAGC 
GGTGGCCCCG CAGCCCCCCC TGACGCCGAG CTGTCACCTC GCTGGAGCAG CAGTGCAGCG 
GCTGTGGCTG TGCAGCAGTG CCATGAAAAT GATTCACCCC AATTAGAACC TCTGGAGGCA 
GAGGGAGAGC CTTTCCCAGA TGCCACCACC ACTGCCAAGC AGCTGCATTC CTCGCCGGGA 
AATTCCTCCA GGCAAGAGAA CGCAGAGACG CCCGCCCGCA GTCCGGGGGA GGACGCTTCA 
CCAGGTGCTG GCCACGAACA GGAGGCTTTC CTGGGTGTGA GGGGTGCGCC AGGGTCGCCC 
ACCCAGGAGC GGCCCGCGGG AGGACTAGGC GAGGCCCCTA ACGGAGCCCC CAGTGTGTGT 
GCCGAAGAAG GCTCCCTGGG GCCCCGCAAC GCCCGCAGCC AGCCCCCCAA GGGCGCGTCT 
GATTTGCCAG GTGAGCCTCC GGCCGAGGGC GCAGCGCACA CGGCCAGCTC CGCGCAGGCA 
GACTGCACAG CCCGCCCCAA GGGTCACGCC CACCCTGCTA AGGTGCTAAC TTTGGACATC 
TACTTGAGTA AGACTGAGGG GGCACAAGTG GACGAGCCGG TCGTGATTAC TCCCAGAGCG 
GAAGATTGCG GTGACTGGGA CGACATGGAG AAGAGGTCCA GCGGCCGTAG GTCGGGGAGG 
CGGAGGGGGT CGCAGAAATC CACCGACTCC CCCGGCGCGG ACGCCGAGCT CCCTGAGAGC 
GCTGCCAGGG ACGACGCGGT GTTCGACGAC GAGGTGGCGC CAAACGCGGC CAGCGATAAC 
GCCTCGGCGG AAAAGAAAGT GAAATCTCCG CGGGCAGCCC TCGACGGGGG CGTTGCCTCC 
GCTGCGAGCC CAGAGTCCAA GCCCAGCCCC GGTACCAAAG GGCAGCTCCG AGGGGAGTCG 
GACCGGAGCA AACAGCCACC CCCGGCTTCG TCCCCCACGA AGAGGAAGGG CAGGAGCCGT 
GCCCTCGAGG CCGTGCCCGC CCCGCCCGCC AGCGGCCCCC GGGCTCCCGC CAAGGAGTCC 
CCACCCAAGA GGGTGCCCGA TCCCAGCCCA GTCACCAAGG GCACTGCGGC CGAGAGCGGG 
GAGGAGGCGG CGCGGGCCAT CCCCCGCGAG CTCCCGGTCA AGAGCAGCTC GCTGCTGCCG 
GAGATCAAGC CCGAGCACAA GAGGGGCCCG CTCCCCAACC ACTTCAACGG CCGGGCAGAG 
GGAGGTCGAA GCAGAGAGCT GGGCAGAGCG GCCGGAGCGC CTGGAGCTTC TGACGCCGAC 
GGCTTGAAGC CCAGGAACCA TTTCGGCGTG GGCAGGTCGA CAGTGACCAC TAAAGTGACC 
CTCCCTGCCA AGCCCAAACA TGTGGAACTA AATCTTAAAA CCCCTAAGAA TCTTGACAGT 
TTGGGAAATG AGCACAATCC ATTTAGCCAG CCAGTTCACA AAGGCAACAC TGCCACCAAA 
ATCTCCTTAT TTGAAAACAA ACGGACAAAC AGTAGCCCAA GACACACTGA CATTCGAGGC 
CAAAGGAATA CTCCTGCCTC TAGTAAAACG TTTGTTGGGA GGGCAAAGCT GAATTTAGCC 
AAAAAAGCCA AAGAAATGGA GCAACCTGAA AAGAAAGTAA TGCCAAACAG TCCCCAGAAT 
GGTGTGCTGG TTAAGGAAAC TGCTATAGAA ACCAAAGTTA CCGTCTCGGA AGAAGAGATT 
CTGCCAGCAA CCAGAGGAAT GAATGGAGAC TCTTCTGAGA ATCAAGCTCT TGGTCCTCAG 
CCTAACCAAG ATGATAAAGC AGATGTACAA ACAGATGCTG GCTGCCTTTC AGAACCAGTG 
GCTTCTGCTC TGATTCCTGT CAAGGATCAT AAGCTCTTAG AGAAGGAGGA CTCAGAGGCT 
GCAGACAGCA AAAGCCTTGT ACTTGAAAAT GTAACCGATA CAGCACAAGA CATCCCCACC 
ACTGTGGATA CCAAAGATTT ACCTCCAACG GCCATGCCAA AGCCACAGCA TACATTTTCT 
GACTCACAGT CCCCTGCTGA GTCATCTCCT GGGCCTTCTC TTTCACTGTC TGCACCCGCT 
CCTGGGGATG TTCCCAAAGA CACATGTGTT CAATCACCCA TAAGCAGTTT CCCATGCACT 
GATCTAAAAG TGTCAGAAAA CCATAAAGGA TGTGTTTTGC CTGTGTCTCG TCAGAACAAT 
GAGAAAATGC CACTTTTAGA ACTTGGAGGA GAAACAACCC CTCCTTTGTC CACAGAGCGT 
AGTCCAGAAG CTGTGGGAAG TGAGTGTCCA TCCAGAGTCC TCGTCCAGGT CAGGTCCTTC 
GTGCTCCCCG TGGAGAGCAC CCAGGATGTG AGCTCCCAGG TCATCCCAGA GAGCTCTGAA 
GTTAGAGAAG TGCAGTTGCC AACTTGTCAC AGTAATGAAC CTGAAGTGGT TTCCGTTGCA 
AGTTGTGCTC CCCCACAAGA GGAAGTACTG GGCAATGAAC ACTCTCATTG CACAGCAGAG 
CTCGCGGCAA AATCTGGCCC ACAAGTCATA CCGCCAGCAT CAGAGAAAAC TCTGCCTATT 
CAGGCTCAAA GTCAGGGCAG CAGAACACCC CTGATGGCTG AATCCAGTCC CACCAACTCT 
CCCAGCAGCG GAAATCACTT AGCCACTCCT CAAAGGCCAG ATCAGACTGT TACAAATGGC 
CAGGATAGCC CTGCCAGCCT TTTGAACATT TCTGCTGGTA GTGATGATAG TGTATTTGAT 
TCTTCTTCTG ATATGGAAAA ATTCACTGAA ATTATAAAAC AGATGGATAG CGCAGTTTGT 
ATGCCCATGA AAAGAAAGAA GGCCAGGATG CCAAACTCTC CTGCTCCTCA CTTTGCCATG 
CCTCCTATTC ACGAAGACCA TTTAGAAAAG GTGTTTGATC CCAAAGTGTT TACCTTTGGT 
TTGGGGAAGA AGAAGGAAAG TCAGCCAGAA ATGTCACCGG CTTTACATTT GATGCAGAAC 
CTTGACACAA AATCCAAACT GAGACCCAAA CGTGCATCTG CTGAACAGAG CGTCCTCTTC 
AAGTCCCTGC ACACCAACAC TAATGGGAAC AGTGAGCCTC TGGTGATGCC GGAAATCAAT 
GACAAAGAGA ACAGGGACGT CACAAATGGT GGCATTAAGA GATCGAGACT AGAAAAAAGT 
GCACTTTTCT CAAGCTTGTT ATCTTCTTTA CCACAAGACA AAATCTTTTC TCCTTCTGTG 
ACATCAGTCA ACACTATGAC CACGGCTTTC AGTACTTCTC AGAACGGTTC CCTATCTCAG 
TCTTCAGTGT CACAGCCCAC GACTGAGGGT GCCCCGCCCT GTGGTTTGAA CAAAGAACAG 
TCAAATCTTC TGCCCGACAA CTCCTTAAAG GTCTTCAATT TCAACTCGTC AAGTACATCA 
CACTCCAGTT TGAAAAGTCC AAGCCACATG GAAAAATACC CGCAAAAAGA GAAAACCAAA 
GAAGATCTGG ATTCACGAAG CAACCTACAC TTGCCAGAAA CTAAATTTTC TGAATTGTCA 
AAACTGAAGA ATGATGATAT GGAAAAGGCT AATCATATTG AAAGTGTTAT TAAATCAAAC 
TTGCCAAACT GTGCAAACAG TGACACCGAC TTCATGGGTC TTTTCAAATC AAGCCGGTAT 
GACCCAAGCA TTTCTTTTTC TGGAATGTCA TTATCAGACA CAATGACACT TAGAGGAAGT 
GTCCAAAATA AACTCAATCC CCGACCTGGA AAGGTAGTGA TATATAGTGA ACCCGACGTC 
TCTGAGAAGT GCATTGAAGT TTTCAGTGAC ATTCAGGATT GCAGTTCTTG GAGCCTCTCT 
CCAGTGATAC TCATAAAAGT TGTTAGAGGA TGTTGGATTT TGTATGAGCA ACCAAATTTT 
GAAGGGCACT CCATCCCCTT AGAAGAAGGA GAATTGGAAC TCTCTGGTCT CTGGGGTATA 
GAAGACATTT TGGAAAGGCA CGAAGAAGCA GAGTCTGATA AGCCAGTGGT GATTGGTTCC 
ATCAGACATG TGGTTCAGGA TTACAGAGTT AGTCACATTG ACTTATTTAC TGAACCAGAA 
GGGTTAGGAA TCCTAAGTTC CTACTTTGAT GATACTGAAG AAATGCAGGG ATTTGGTGTA 
ATGCAGAAGA CTTGTTCCAT GAAAGTACAT TGGGGCACGT GGCTGATTTA TGAAGAACCT 
GGATTTCAGG GTGTTCCTTT CATCCTGGAA CCTGGTGAAT ACCCTGACTT GTCCTTCTGG 
GATACAGAAG AAGCGTACAT TGGATCCATG CGGCCTCTGA AAATGGGTGG CCGTAAAGTT 
GAATTCCCTA CAGATCCAAA GGTAGTTGTT TATGAAAAGC CTTTCTTTGA AGGAAAATGT 
GTGGAACTAG AAACAGGAAT GTGTAGTTTT GTCATGGAGG GAGGTGAAAC AGAAGAGGCG 
ACTGGAGACG ATCATTTGCC GTTTACGTCA GTGGGGTCTA TGAAAGTTCT AAGAGGCATT 
TGGGTTGCAT ATGAGAAACC TGGATTTACC GGTCATCAGT ATTTGCTAGA AGAAGGAGAA 
TACAGGGACT GGAAAGCCTG GGGAGGTTAC AATGGAGAGC TTCAGTCTTT ACGACCTATA 
TTAGGTGATT TTTCAAATGC TCACATGATA ATGTACAGTG AAAAAAACTT TGGATCCAAA 
GGTTCCAGTA TTGATGTATT GGGAATTGTT GCTAATTTAA AGGAGACTGG ATATGGAGTG 
AAGACACAGT CTATTAATGT ACTGAGTGGA GTATGGGTAG CCTATGAAAA TCCTGACTTC 
ACAGGAGAAC AGTATATACT GGATAAAGGA TTTTATACCA GTTTTGAGGA CTGGGGAGGC 
AAAAATTGTA AGATCTCTTC TGTTCAACCT ATATGTTTGG ATTCTTTCAC TGGCCCAAGG 
AGACGAAATC AGATTCACTT GTTTTCAGAA CCACAGTTTC AAGGTCACAG TCAAAGTTTT 
GAAGAAACAA CAAGTCAAAT TGATGATTCA TTTTCTACCA AGTCTTGCAG AGTTTCAGGA 
GGCAGCTGGG TTGTATATGA TGGAGAAAAT TTCACTGGTA ATCAATACGT GTTGGAAGAA 
GGCCATTATC CTTGTCTGTC TGCAATGGGA TGCCCGCCTG GAGCAACTTT CAAGTCTCTT 
CGTTTTATAG ATGTTGAATT TTCTGAACCA ACAATTATTC TCTTTGAAAG AGAAGACTTC 
AAAGGAAAAA AGATTGAACT TAATGCAGAA ACTGTCAATC TCCGATCCCT GGGATTCAAC 
ACACAAATAC GCTCTGTTCA GGTTATTGGT GGCATATGGG TTACTTATGA ATATGGCAGT 
TACAGAGGGC GACAGTTCCT ATTGTCACCT GCAGAAGTAC CTAATTGGTA TGAATTCAGT 
GGCTGTCGCC AAATAGGTTC TCTACGACCT TTTGTTCAGA AGCGAATTTA TTTCAGACTT 
CGAAACAAAG CAACAGGGTT ATTCATGTCA ACCAATGGAA ACTTAGAGGA TCTGAAGCTT 
CTGAGGATAC AGGTCATGGA GGATGTCGGG GCCGATGATC AGATTTGGAT CTATCAAGAA 
GGATGTATCA AATGCAGGAT AGCAGAAGAC TGCTGCCTGA CGATTGTGGG CAGCCTGGTA 
ACATCTGGCT CCAAGCTAGG CCTGGCCCTG GACCAGAATG CTGACAGCCA GTTCTGGAGC 
TTGAAGTCCG ATGGCAGGAT TTACAGCAAG TTGAAGCCAA ATTTAGTTTT AGACATTAAA 
GGGGGCACAC AGTATGATCA AAATCACATT ATCCTCAACA CTGTCAGCAA AGAGAAGTTT 
ACACAAGTGT GGGAAGCCAT GGTCCTATAT ACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CRYBG1 ( NM_001371242.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001358171.1 Homo sapiens crystallin beta-gamma domain containing 1 (CRYBG1), transcript variant 1, mRNA.

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CloneID	OHu40642
Clone ID Related Accession (Same CDS sequence)	NM_001371242.1 , XM_005266839.3 , NM_001371242.2
Accession Version	NM_001371242.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6396bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-02
Organism	Homo sapiens(human)
Product	beta/gamma crystallin domain-containing protein 1 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL109920.15, AL359292.12 and AL390074.17. On Jul 2, 2019 this sequence version replaced XM_005266839.3. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology RefSeq Select criteria :: based on manual assertion, conservation, longest protein ##RefSeq-Attributes-END##

ATGCCGCTGT CCCCGCCAGC CCAGGGCGAC CCCGGGGAGC CCAGCCCGTG CAGGCCCCCT 
AAGAAGCACA CCACCTTCCA CCTCTGGCGC TCCAAAAAGA AGCAGCAGCC GGCGCCGCCT 
GACTGTGGGG TGTTCGTTCC GCACCCGCTC CCGGCGCCTG CCGGAGAGGC CAGAGCTTTG 
GATGTAGTCG ATGGAAAATA TGTGGTTCGA GACTCCCAGG AATTTCCACT GCACTGTGGG 
GAATCCCAGT TCTTCCACAC CACCAGTGAG GCGCTTGGTT CCTTACTTCT AGAGTCTGGA 
ATATTTAAAA AGTCCAGAGC ACAACCTCCA GAAGACAACA GAAGGAAGCC AGTTTTGGGG 
AAACTTGGCA CTCTATTCAC TGCAGGAAGG AGAAGAAACA GTAGAAACGG GTTAGAGAGT 
CCCACCAGAT CAAATGCCAA ACCACTCTCT CCCAAAGATG TGGTAGCCTC TCCTAAGCTC 
CCAGAGAGAG AGAGTGAGAG GAGCAGATCT CAGAGCAGCC AACTGAAGCA AACGGACACA 
AGCGAGGAGG GCTCCCCGCG GGAGAATCCC CGAGAGGCAG AGGGCGAGCT CCCCGAGAGC 
GGTGGCCCCG CAGCCCCCCC TGACGCCGAG CTGTCACCTC GCTGGAGCAG CAGTGCAGCG 
GCTGTGGCTG TGCAGCAGTG CCATGAAAAT GATTCACCCC AATTAGAACC TCTGGAGGCA 
GAGGGAGAGC CTTTCCCAGA TGCCACCACC ACTGCCAAGC AGCTGCATTC CTCGCCGGGA 
AATTCCTCCA GGCAAGAGAA CGCAGAGACG CCCGCCCGCA GTCCGGGGGA GGACGCTTCA 
CCAGGTGCTG GCCACGAACA GGAGGCTTTC CTGGGTGTGA GGGGTGCGCC AGGGTCGCCC 
ACCCAGGAGC GGCCCGCGGG AGGACTAGGC GAGGCCCCTA ACGGAGCCCC CAGTGTGTGT 
GCCGAAGAAG GCTCCCTGGG GCCCCGCAAC GCCCGCAGCC AGCCCCCCAA GGGCGCGTCT 
GATTTGCCAG GTGAGCCTCC GGCCGAGGGC GCAGCGCACA CGGCCAGCTC CGCGCAGGCA 
GACTGCACAG CCCGCCCCAA GGGTCACGCC CACCCTGCTA AGGTGCTAAC TTTGGACATC 
TACTTGAGTA AGACTGAGGG GGCACAAGTG GACGAGCCGG TCGTGATTAC TCCCAGAGCG 
GAAGATTGCG GTGACTGGGA CGACATGGAG AAGAGGTCCA GCGGCCGTAG GTCGGGGAGG 
CGGAGGGGGT CGCAGAAATC CACCGACTCC CCCGGCGCGG ACGCCGAGCT CCCTGAGAGC 
GCTGCCAGGG ACGACGCGGT GTTCGACGAC GAGGTGGCGC CAAACGCGGC CAGCGATAAC 
GCCTCGGCGG AAAAGAAAGT GAAATCTCCG CGGGCAGCCC TCGACGGGGG CGTTGCCTCC 
GCTGCGAGCC CAGAGTCCAA GCCCAGCCCC GGTACCAAAG GGCAGCTCCG AGGGGAGTCG 
GACCGGAGCA AACAGCCACC CCCGGCTTCG TCCCCCACGA AGAGGAAGGG CAGGAGCCGT 
GCCCTCGAGG CCGTGCCCGC CCCGCCCGCC AGCGGCCCCC GGGCTCCCGC CAAGGAGTCC 
CCACCCAAGA GGGTGCCCGA TCCCAGCCCA GTCACCAAGG GCACTGCGGC CGAGAGCGGG 
GAGGAGGCGG CGCGGGCCAT CCCCCGCGAG CTCCCGGTCA AGAGCAGCTC GCTGCTGCCG 
GAGATCAAGC CCGAGCACAA GAGGGGCCCG CTCCCCAACC ACTTCAACGG CCGGGCAGAG 
GGAGGTCGAA GCAGAGAGCT GGGCAGAGCG GCCGGAGCGC CTGGAGCTTC TGACGCCGAC 
GGCTTGAAGC CCAGGAACCA TTTCGGCGTG GGCAGGTCGA CAGTGACCAC TAAAGTGACC 
CTCCCTGCCA AGCCCAAACA TGTGGAACTA AATCTTAAAA CCCCTAAGAA TCTTGACAGT 
TTGGGAAATG AGCACAATCC ATTTAGCCAG CCAGTTCACA AAGGCAACAC TGCCACCAAA 
ATCTCCTTAT TTGAAAACAA ACGGACAAAC AGTAGCCCAA GACACACTGA CATTCGAGGC 
CAAAGGAATA CTCCTGCCTC TAGTAAAACG TTTGTTGGGA GGGCAAAGCT GAATTTAGCC 
AAAAAAGCCA AAGAAATGGA GCAACCTGAA AAGAAAGTAA TGCCAAACAG TCCCCAGAAT 
GGTGTGCTGG TTAAGGAAAC TGCTATAGAA ACCAAAGTTA CCGTCTCGGA AGAAGAGATT 
CTGCCAGCAA CCAGAGGAAT GAATGGAGAC TCTTCTGAGA ATCAAGCTCT TGGTCCTCAG 
CCTAACCAAG ATGATAAAGC AGATGTACAA ACAGATGCTG GCTGCCTTTC AGAACCAGTG 
GCTTCTGCTC TGATTCCTGT CAAGGATCAT AAGCTCTTAG AGAAGGAGGA CTCAGAGGCT 
GCAGACAGCA AAAGCCTTGT ACTTGAAAAT GTAACCGATA CAGCACAAGA CATCCCCACC 
ACTGTGGATA CCAAAGATTT ACCTCCAACG GCCATGCCAA AGCCACAGCA TACATTTTCT 
GACTCACAGT CCCCTGCTGA GTCATCTCCT GGGCCTTCTC TTTCACTGTC TGCACCCGCT 
CCTGGGGATG TTCCCAAAGA CACATGTGTT CAATCACCCA TAAGCAGTTT CCCATGCACT 
GATCTAAAAG TGTCAGAAAA CCATAAAGGA TGTGTTTTGC CTGTGTCTCG TCAGAACAAT 
GAGAAAATGC CACTTTTAGA ACTTGGAGGA GAAACAACCC CTCCTTTGTC CACAGAGCGT 
AGTCCAGAAG CTGTGGGAAG TGAGTGTCCA TCCAGAGTCC TCGTCCAGGT CAGGTCCTTC 
GTGCTCCCCG TGGAGAGCAC CCAGGATGTG AGCTCCCAGG TCATCCCAGA GAGCTCTGAA 
GTTAGAGAAG TGCAGTTGCC AACTTGTCAC AGTAATGAAC CTGAAGTGGT TTCCGTTGCA 
AGTTGTGCTC CCCCACAAGA GGAAGTACTG GGCAATGAAC ACTCTCATTG CACAGCAGAG 
CTCGCGGCAA AATCTGGCCC ACAAGTCATA CCGCCAGCAT CAGAGAAAAC TCTGCCTATT 
CAGGCTCAAA GTCAGGGCAG CAGAACACCC CTGATGGCTG AATCCAGTCC CACCAACTCT 
CCCAGCAGCG GAAATCACTT AGCCACTCCT CAAAGGCCAG ATCAGACTGT TACAAATGGC 
CAGGATAGCC CTGCCAGCCT TTTGAACATT TCTGCTGGTA GTGATGATAG TGTATTTGAT 
TCTTCTTCTG ATATGGAAAA ATTCACTGAA ATTATAAAAC AGATGGATAG CGCAGTTTGT 
ATGCCCATGA AAAGAAAGAA GGCCAGGATG CCAAACTCTC CTGCTCCTCA CTTTGCCATG 
CCTCCTATTC ACGAAGACCA TTTAGAAAAG GTGTTTGATC CCAAAGTGTT TACCTTTGGT 
TTGGGGAAGA AGAAGGAAAG TCAGCCAGAA ATGTCACCGG CTTTACATTT GATGCAGAAC 
CTTGACACAA AATCCAAACT GAGACCCAAA CGTGCATCTG CTGAACAGAG CGTCCTCTTC 
AAGTCCCTGC ACACCAACAC TAATGGGAAC AGTGAGCCTC TGGTGATGCC GGAAATCAAT 
GACAAAGAGA ACAGGGACGT CACAAATGGT GGCATTAAGA GATCGAGACT AGAAAAAAGT 
GCACTTTTCT CAAGCTTGTT ATCTTCTTTA CCACAAGACA AAATCTTTTC TCCTTCTGTG 
ACATCAGTCA ACACTATGAC CACGGCTTTC AGTACTTCTC AGAACGGTTC CCTATCTCAG 
TCTTCAGTGT CACAGCCCAC GACTGAGGGT GCCCCGCCCT GTGGTTTGAA CAAAGAACAG 
TCAAATCTTC TGCCCGACAA CTCCTTAAAG GTCTTCAATT TCAACTCGTC AAGTACATCA 
CACTCCAGTT TGAAAAGTCC AAGCCACATG GAAAAATACC CGCAAAAAGA GAAAACCAAA 
GAAGATCTGG ATTCACGAAG CAACCTACAC TTGCCAGAAA CTAAATTTTC TGAATTGTCA 
AAACTGAAGA ATGATGATAT GGAAAAGGCT AATCATATTG AAAGTGTTAT TAAATCAAAC 
TTGCCAAACT GTGCAAACAG TGACACCGAC TTCATGGGTC TTTTCAAATC AAGCCGGTAT 
GACCCAAGCA TTTCTTTTTC TGGAATGTCA TTATCAGACA CAATGACACT TAGAGGAAGT 
GTCCAAAATA AACTCAATCC CCGACCTGGA AAGGTAGTGA TATATAGTGA ACCCGACGTC 
TCTGAGAAGT GCATTGAAGT TTTCAGTGAC ATTCAGGATT GCAGTTCTTG GAGCCTCTCT 
CCAGTGATAC TCATAAAAGT TGTTAGAGGA TGTTGGATTT TGTATGAGCA ACCAAATTTT 
GAAGGGCACT CCATCCCCTT AGAAGAAGGA GAATTGGAAC TCTCTGGTCT CTGGGGTATA 
GAAGACATTT TGGAAAGGCA CGAAGAAGCA GAGTCTGATA AGCCAGTGGT GATTGGTTCC 
ATCAGACATG TGGTTCAGGA TTACAGAGTT AGTCACATTG ACTTATTTAC TGAACCAGAA 
GGGTTAGGAA TCCTAAGTTC CTACTTTGAT GATACTGAAG AAATGCAGGG ATTTGGTGTA 
ATGCAGAAGA CTTGTTCCAT GAAAGTACAT TGGGGCACGT GGCTGATTTA TGAAGAACCT 
GGATTTCAGG GTGTTCCTTT CATCCTGGAA CCTGGTGAAT ACCCTGACTT GTCCTTCTGG 
GATACAGAAG AAGCGTACAT TGGATCCATG CGGCCTCTGA AAATGGGTGG CCGTAAAGTT 
GAATTCCCTA CAGATCCAAA GGTAGTTGTT TATGAAAAGC CTTTCTTTGA AGGAAAATGT 
GTGGAACTAG AAACAGGAAT GTGTAGTTTT GTCATGGAGG GAGGTGAAAC AGAAGAGGCG 
ACTGGAGACG ATCATTTGCC GTTTACGTCA GTGGGGTCTA TGAAAGTTCT AAGAGGCATT 
TGGGTTGCAT ATGAGAAACC TGGATTTACC GGTCATCAGT ATTTGCTAGA AGAAGGAGAA 
TACAGGGACT GGAAAGCCTG GGGAGGTTAC AATGGAGAGC TTCAGTCTTT ACGACCTATA 
TTAGGTGATT TTTCAAATGC TCACATGATA ATGTACAGTG AAAAAAACTT TGGATCCAAA 
GGTTCCAGTA TTGATGTATT GGGAATTGTT GCTAATTTAA AGGAGACTGG ATATGGAGTG 
AAGACACAGT CTATTAATGT ACTGAGTGGA GTATGGGTAG CCTATGAAAA TCCTGACTTC 
ACAGGAGAAC AGTATATACT GGATAAAGGA TTTTATACCA GTTTTGAGGA CTGGGGAGGC 
AAAAATTGTA AGATCTCTTC TGTTCAACCT ATATGTTTGG ATTCTTTCAC TGGCCCAAGG 
AGACGAAATC AGATTCACTT GTTTTCAGAA CCACAGTTTC AAGGTCACAG TCAAAGTTTT 
GAAGAAACAA CAAGTCAAAT TGATGATTCA TTTTCTACCA AGTCTTGCAG AGTTTCAGGA 
GGCAGCTGGG TTGTATATGA TGGAGAAAAT TTCACTGGTA ATCAATACGT GTTGGAAGAA 
GGCCATTATC CTTGTCTGTC TGCAATGGGA TGCCCGCCTG GAGCAACTTT CAAGTCTCTT 
CGTTTTATAG ATGTTGAATT TTCTGAACCA ACAATTATTC TCTTTGAAAG AGAAGACTTC 
AAAGGAAAAA AGATTGAACT TAATGCAGAA ACTGTCAATC TCCGATCCCT GGGATTCAAC 
ACACAAATAC GCTCTGTTCA GGTTATTGGT GGCATATGGG TTACTTATGA ATATGGCAGT 
TACAGAGGGC GACAGTTCCT ATTGTCACCT GCAGAAGTAC CTAATTGGTA TGAATTCAGT 
GGCTGTCGCC AAATAGGTTC TCTACGACCT TTTGTTCAGA AGCGAATTTA TTTCAGACTT 
CGAAACAAAG CAACAGGGTT ATTCATGTCA ACCAATGGAA ACTTAGAGGA TCTGAAGCTT 
CTGAGGATAC AGGTCATGGA GGATGTCGGG GCCGATGATC AGATTTGGAT CTATCAAGAA 
GGATGTATCA AATGCAGGAT AGCAGAAGAC TGCTGCCTGA CGATTGTGGG CAGCCTGGTA 
ACATCTGGCT CCAAGCTAGG CCTGGCCCTG GACCAGAATG CTGACAGCCA GTTCTGGAGC 
TTGAAGTCCG ATGGCAGGAT TTACAGCAAG TTGAAGCCAA ATTTAGTTTT AGACATTAAA 
GGGGGCACAC AGTATGATCA AAATCACATT ATCCTCAACA CTGTCAGCAA AGAGAAGTTT 
ACACAAGTGT GGGAAGCCAT GGTCCTATAT ACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001358171.1
CDS	193..6588
Translation	MPLSPPAQGDPGEPSPCRPPKKHTTFHLWRSKKKQQPAPPDCGVFVPHPLPAPAGEARALDVVDGKYVVRDSQEFPLHCGESQFFHTTSEALGSLLLESGIFKKSRAQPPEDNRRKPVLGKLGTLFTAGRRRNSRNGLESPTRSNAKPLSPKDVVASPKLPERESERSRSQSSQLKQTDTSEEGSPRENPREAEGELPESGGPAAPPDAELSPRWSSSAAAVAVQQCHENDSPQLEPLEAEGEPFPDATTTAKQLHSSPGNSSRQENAETPARSPGEDASPGAGHEQEAFLGVRGAPGSPTQERPAGGLGEAPNGAPSVCAEEGSLGPRNARSQPPKGASDLPGEPPAEGAAHTASSAQADCTARPKGHAHPAKVLTLDIYLSKTEGAQVDEPVVITPRAEDCGDWDDMEKRSSGRRSGRRRGSQKSTDSPGADAELPESAARDDAVFDDEVAPNAASDNASAEKKVKSPRAALDGGVASAASPESKPSPGTKGQLRGESDRSKQPPPASSPTKRKGRSRALEAVPAPPASGPRAPAKESPPKRVPDPSPVTKGTAAESGEEAARAIPRELPVKSSSLLPEIKPEHKRGPLPNHFNGRAEGGRSRELGRAAGAPGASDADGLKPRNHFGVGRSTVTTKVTLPAKPKHVELNLKTPKNLDSLGNEHNPFSQPVHKGNTATKISLFENKRTNSSPRHTDIRGQRNTPASSKTFVGRAKLNLAKKAKEMEQPEKKVMPNSPQNGVLVKETAIETKVTVSEEEILPATRGMNGDSSENQALGPQPNQDDKADVQTDAGCLSEPVASALIPVKDHKLLEKEDSEAADSKSLVLENVTDTAQDIPTTVDTKDLPPTAMPKPQHTFSDSQSPAESSPGPSLSLSAPAPGDVPKDTCVQSPISSFPCTDLKVSENHKGCVLPVSRQNNEKMPLLELGGETTPPLSTERSPEAVGSECPSRVLVQVRSFVLPVESTQDVSSQVIPESSEVREVQLPTCHSNEPEVVSVASCAPPQEEVLGNEHSHCTAELAAKSGPQVIPPASEKTLPIQAQSQGSRTPLMAESSPTNSPSSGNHLATPQRPDQTVTNGQDSPASLLNISAGSDDSVFDSSSDMEKFTEIIKQMDSAVCMPMKRKKARMPNSPAPHFAMPPIHEDHLEKVFDPKVFTFGLGKKKESQPEMSPALHLMQNLDTKSKLRPKRASAEQSVLFKSLHTNTNGNSEPLVMPEINDKENRDVTNGGIKRSRLEKSALFSSLLSSLPQDKIFSPSVTSVNTMTTAFSTSQNGSLSQSSVSQPTTEGAPPCGLNKEQSNLLPDNSLKVFNFNSSSTSHSSLKSPSHMEKYPQKEKTKEDLDSRSNLHLPETKFSELSKLKNDDMEKANHIESVIKSNLPNCANSDTDFMGLFKSSRYDPSISFSGMSLSDTMTLRGSVQNKLNPRPGKVVIYSEPDVSEKCIEVFSDIQDCSSWSLSPVILIKVVRGCWILYEQPNFEGHSIPLEEGELELSGLWGIEDILERHEEAESDKPVVIGSIRHVVQDYRVSHIDLFTEPEGLGILSSYFDDTEEMQGFGVMQKTCSMKVHWGTWLIYEEPGFQGVPFILEPGEYPDLSFWDTEEAYIGSMRPLKMGGRKVEFPTDPKVVVYEKPFFEGKCVELETGMCSFVMEGGETEEATGDDHLPFTSVGSMKVLRGIWVAYEKPGFTGHQYLLEEGEYRDWKAWGGYNGELQSLRPILGDFSNAHMIMYSEKNFGSKGSSIDVLGIVANLKETGYGVKTQSINVLSGVWVAYENPDFTGEQYILDKGFYTSFEDWGGKNCKISSVQPICLDSFTGPRRRNQIHLFSEPQFQGHSQSFEETTSQIDDSFSTKSCRVSGGSWVVYDGENFTGNQYVLEEGHYPCLSAMGCPPGATFKSLRFIDVEFSEPTIILFEREDFKGKKIELNAETVNLRSLGFNTQIRSVQVIGGIWVTYEYGSYRGRQFLLSPAEVPNWYEFSGCRQIGSLRPFVQKRIYFRLRNKATGLFMSTNGNLEDLKLLRIQVMEDVGADDQIWIYQEGCIKCRIAEDCCLTIVGSLVTSGSKLGLALDQNADSQFWSLKSDGRIYSKLKPNLVLDIKGGTQYDQNHIILNTVSKEKFTQVWEAMVLYT

Target ORF information:

RefSeq Version	NM_001371242.1
Organism	Homo sapiens(human)
Definition	Homo sapiens crystallin beta-gamma domain containing 1 (CRYBG1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001371242.1

ORF Insert Sequence:

ATGCCGCTGT CCCCGCCAGC CCAGGGCGAC CCCGGGGAGC CCAGCCCGTG CAGGCCCCCT 
AAGAAGCACA CCACCTTCCA CCTCTGGCGC TCCAAAAAGA AGCAGCAGCC GGCGCCGCCT 
GACTGTGGGG TGTTCGTTCC GCACCCGCTC CCGGCGCCTG CCGGAGAGGC CAGAGCTTTG 
GATGTAGTCG ATGGAAAATA TGTGGTTCGA GACTCCCAGG AATTTCCACT GCACTGTGGG 
GAATCCCAGT TCTTCCACAC CACCAGTGAG GCGCTTGGTT CCTTACTTCT AGAGTCTGGA 
ATATTTAAAA AGTCCAGAGC ACAACCTCCA GAAGACAACA GAAGGAAGCC AGTTTTGGGG 
AAACTTGGCA CTCTATTCAC TGCAGGAAGG AGAAGAAACA GTAGAAACGG GTTAGAGAGT 
CCCACCAGAT CAAATGCCAA ACCACTCTCT CCCAAAGATG TGGTAGCCTC TCCTAAGCTC 
CCAGAGAGAG AGAGTGAGAG GAGCAGATCT CAGAGCAGCC AACTGAAGCA AACGGACACA 
AGCGAGGAGG GCTCCCCGCG GGAGAATCCC CGAGAGGCAG AGGGCGAGCT CCCCGAGAGC 
GGTGGCCCCG CAGCCCCCCC TGACGCCGAG CTGTCACCTC GCTGGAGCAG CAGTGCAGCG 
GCTGTGGCTG TGCAGCAGTG CCATGAAAAT GATTCACCCC AATTAGAACC TCTGGAGGCA 
GAGGGAGAGC CTTTCCCAGA TGCCACCACC ACTGCCAAGC AGCTGCATTC CTCGCCGGGA 
AATTCCTCCA GGCAAGAGAA CGCAGAGACG CCCGCCCGCA GTCCGGGGGA GGACGCTTCA 
CCAGGTGCTG GCCACGAACA GGAGGCTTTC CTGGGTGTGA GGGGTGCGCC AGGGTCGCCC 
ACCCAGGAGC GGCCCGCGGG AGGACTAGGC GAGGCCCCTA ACGGAGCCCC CAGTGTGTGT 
GCCGAAGAAG GCTCCCTGGG GCCCCGCAAC GCCCGCAGCC AGCCCCCCAA GGGCGCGTCT 
GATTTGCCAG GTGAGCCTCC GGCCGAGGGC GCAGCGCACA CGGCCAGCTC CGCGCAGGCA 
GACTGCACAG CCCGCCCCAA GGGTCACGCC CACCCTGCTA AGGTGCTAAC TTTGGACATC 
TACTTGAGTA AGACTGAGGG GGCACAAGTG GACGAGCCGG TCGTGATTAC TCCCAGAGCG 
GAAGATTGCG GTGACTGGGA CGACATGGAG AAGAGGTCCA GCGGCCGTAG GTCGGGGAGG 
CGGAGGGGGT CGCAGAAATC CACCGACTCC CCCGGCGCGG ACGCCGAGCT CCCTGAGAGC 
GCTGCCAGGG ACGACGCGGT GTTCGACGAC GAGGTGGCGC CAAACGCGGC CAGCGATAAC 
GCCTCGGCGG AAAAGAAAGT GAAATCTCCG CGGGCAGCCC TCGACGGGGG CGTTGCCTCC 
GCTGCGAGCC CAGAGTCCAA GCCCAGCCCC GGTACCAAAG GGCAGCTCCG AGGGGAGTCG 
GACCGGAGCA AACAGCCACC CCCGGCTTCG TCCCCCACGA AGAGGAAGGG CAGGAGCCGT 
GCCCTCGAGG CCGTGCCCGC CCCGCCCGCC AGCGGCCCCC GGGCTCCCGC CAAGGAGTCC 
CCACCCAAGA GGGTGCCCGA TCCCAGCCCA GTCACCAAGG GCACTGCGGC CGAGAGCGGG 
GAGGAGGCGG CGCGGGCCAT CCCCCGCGAG CTCCCGGTCA AGAGCAGCTC GCTGCTGCCG 
GAGATCAAGC CCGAGCACAA GAGGGGCCCG CTCCCCAACC ACTTCAACGG CCGGGCAGAG 
GGAGGTCGAA GCAGAGAGCT GGGCAGAGCG GCCGGAGCGC CTGGAGCTTC TGACGCCGAC 
GGCTTGAAGC CCAGGAACCA TTTCGGCGTG GGCAGGTCGA CAGTGACCAC TAAAGTGACC 
CTCCCTGCCA AGCCCAAACA TGTGGAACTA AATCTTAAAA CCCCTAAGAA TCTTGACAGT 
TTGGGAAATG AGCACAATCC ATTTAGCCAG CCAGTTCACA AAGGCAACAC TGCCACCAAA 
ATCTCCTTAT TTGAAAACAA ACGGACAAAC AGTAGCCCAA GACACACTGA CATTCGAGGC 
CAAAGGAATA CTCCTGCCTC TAGTAAAACG TTTGTTGGGA GGGCAAAGCT GAATTTAGCC 
AAAAAAGCCA AAGAAATGGA GCAACCTGAA AAGAAAGTAA TGCCAAACAG TCCCCAGAAT 
GGTGTGCTGG TTAAGGAAAC TGCTATAGAA ACCAAAGTTA CCGTCTCGGA AGAAGAGATT 
CTGCCAGCAA CCAGAGGAAT GAATGGAGAC TCTTCTGAGA ATCAAGCTCT TGGTCCTCAG 
CCTAACCAAG ATGATAAAGC AGATGTACAA ACAGATGCTG GCTGCCTTTC AGAACCAGTG 
GCTTCTGCTC TGATTCCTGT CAAGGATCAT AAGCTCTTAG AGAAGGAGGA CTCAGAGGCT 
GCAGACAGCA AAAGCCTTGT ACTTGAAAAT GTAACCGATA CAGCACAAGA CATCCCCACC 
ACTGTGGATA CCAAAGATTT ACCTCCAACG GCCATGCCAA AGCCACAGCA TACATTTTCT 
GACTCACAGT CCCCTGCTGA GTCATCTCCT GGGCCTTCTC TTTCACTGTC TGCACCCGCT 
CCTGGGGATG TTCCCAAAGA CACATGTGTT CAATCACCCA TAAGCAGTTT CCCATGCACT 
GATCTAAAAG TGTCAGAAAA CCATAAAGGA TGTGTTTTGC CTGTGTCTCG TCAGAACAAT 
GAGAAAATGC CACTTTTAGA ACTTGGAGGA GAAACAACCC CTCCTTTGTC CACAGAGCGT 
AGTCCAGAAG CTGTGGGAAG TGAGTGTCCA TCCAGAGTCC TCGTCCAGGT CAGGTCCTTC 
GTGCTCCCCG TGGAGAGCAC CCAGGATGTG AGCTCCCAGG TCATCCCAGA GAGCTCTGAA 
GTTAGAGAAG TGCAGTTGCC AACTTGTCAC AGTAATGAAC CTGAAGTGGT TTCCGTTGCA 
AGTTGTGCTC CCCCACAAGA GGAAGTACTG GGCAATGAAC ACTCTCATTG CACAGCAGAG 
CTCGCGGCAA AATCTGGCCC ACAAGTCATA CCGCCAGCAT CAGAGAAAAC TCTGCCTATT 
CAGGCTCAAA GTCAGGGCAG CAGAACACCC CTGATGGCTG AATCCAGTCC CACCAACTCT 
CCCAGCAGCG GAAATCACTT AGCCACTCCT CAAAGGCCAG ATCAGACTGT TACAAATGGC 
CAGGATAGCC CTGCCAGCCT TTTGAACATT TCTGCTGGTA GTGATGATAG TGTATTTGAT 
TCTTCTTCTG ATATGGAAAA ATTCACTGAA ATTATAAAAC AGATGGATAG CGCAGTTTGT 
ATGCCCATGA AAAGAAAGAA GGCCAGGATG CCAAACTCTC CTGCTCCTCA CTTTGCCATG 
CCTCCTATTC ACGAAGACCA TTTAGAAAAG GTGTTTGATC CCAAAGTGTT TACCTTTGGT 
TTGGGGAAGA AGAAGGAAAG TCAGCCAGAA ATGTCACCGG CTTTACATTT GATGCAGAAC 
CTTGACACAA AATCCAAACT GAGACCCAAA CGTGCATCTG CTGAACAGAG CGTCCTCTTC 
AAGTCCCTGC ACACCAACAC TAATGGGAAC AGTGAGCCTC TGGTGATGCC GGAAATCAAT 
GACAAAGAGA ACAGGGACGT CACAAATGGT GGCATTAAGA GATCGAGACT AGAAAAAAGT 
GCACTTTTCT CAAGCTTGTT ATCTTCTTTA CCACAAGACA AAATCTTTTC TCCTTCTGTG 
ACATCAGTCA ACACTATGAC CACGGCTTTC AGTACTTCTC AGAACGGTTC CCTATCTCAG 
TCTTCAGTGT CACAGCCCAC GACTGAGGGT GCCCCGCCCT GTGGTTTGAA CAAAGAACAG 
TCAAATCTTC TGCCCGACAA CTCCTTAAAG GTCTTCAATT TCAACTCGTC AAGTACATCA 
CACTCCAGTT TGAAAAGTCC AAGCCACATG GAAAAATACC CGCAAAAAGA GAAAACCAAA 
GAAGATCTGG ATTCACGAAG CAACCTACAC TTGCCAGAAA CTAAATTTTC TGAATTGTCA 
AAACTGAAGA ATGATGATAT GGAAAAGGCT AATCATATTG AAAGTGTTAT TAAATCAAAC 
TTGCCAAACT GTGCAAACAG TGACACCGAC TTCATGGGTC TTTTCAAATC AAGCCGGTAT 
GACCCAAGCA TTTCTTTTTC TGGAATGTCA TTATCAGACA CAATGACACT TAGAGGAAGT 
GTCCAAAATA AACTCAATCC CCGACCTGGA AAGGTAGTGA TATATAGTGA ACCCGACGTC 
TCTGAGAAGT GCATTGAAGT TTTCAGTGAC ATTCAGGATT GCAGTTCTTG GAGCCTCTCT 
CCAGTGATAC TCATAAAAGT TGTTAGAGGA TGTTGGATTT TGTATGAGCA ACCAAATTTT 
GAAGGGCACT CCATCCCCTT AGAAGAAGGA GAATTGGAAC TCTCTGGTCT CTGGGGTATA 
GAAGACATTT TGGAAAGGCA CGAAGAAGCA GAGTCTGATA AGCCAGTGGT GATTGGTTCC 
ATCAGACATG TGGTTCAGGA TTACAGAGTT AGTCACATTG ACTTATTTAC TGAACCAGAA 
GGGTTAGGAA TCCTAAGTTC CTACTTTGAT GATACTGAAG AAATGCAGGG ATTTGGTGTA 
ATGCAGAAGA CTTGTTCCAT GAAAGTACAT TGGGGCACGT GGCTGATTTA TGAAGAACCT 
GGATTTCAGG GTGTTCCTTT CATCCTGGAA CCTGGTGAAT ACCCTGACTT GTCCTTCTGG 
GATACAGAAG AAGCGTACAT TGGATCCATG CGGCCTCTGA AAATGGGTGG CCGTAAAGTT 
GAATTCCCTA CAGATCCAAA GGTAGTTGTT TATGAAAAGC CTTTCTTTGA AGGAAAATGT 
GTGGAACTAG AAACAGGAAT GTGTAGTTTT GTCATGGAGG GAGGTGAAAC AGAAGAGGCG 
ACTGGAGACG ATCATTTGCC GTTTACGTCA GTGGGGTCTA TGAAAGTTCT AAGAGGCATT 
TGGGTTGCAT ATGAGAAACC TGGATTTACC GGTCATCAGT ATTTGCTAGA AGAAGGAGAA 
TACAGGGACT GGAAAGCCTG GGGAGGTTAC AATGGAGAGC TTCAGTCTTT ACGACCTATA 
TTAGGTGATT TTTCAAATGC TCACATGATA ATGTACAGTG AAAAAAACTT TGGATCCAAA 
GGTTCCAGTA TTGATGTATT GGGAATTGTT GCTAATTTAA AGGAGACTGG ATATGGAGTG 
AAGACACAGT CTATTAATGT ACTGAGTGGA GTATGGGTAG CCTATGAAAA TCCTGACTTC 
ACAGGAGAAC AGTATATACT GGATAAAGGA TTTTATACCA GTTTTGAGGA CTGGGGAGGC 
AAAAATTGTA AGATCTCTTC TGTTCAACCT ATATGTTTGG ATTCTTTCAC TGGCCCAAGG 
AGACGAAATC AGATTCACTT GTTTTCAGAA CCACAGTTTC AAGGTCACAG TCAAAGTTTT 
GAAGAAACAA CAAGTCAAAT TGATGATTCA TTTTCTACCA AGTCTTGCAG AGTTTCAGGA 
GGCAGCTGGG TTGTATATGA TGGAGAAAAT TTCACTGGTA ATCAATACGT GTTGGAAGAA 
GGCCATTATC CTTGTCTGTC TGCAATGGGA TGCCCGCCTG GAGCAACTTT CAAGTCTCTT 
CGTTTTATAG ATGTTGAATT TTCTGAACCA ACAATTATTC TCTTTGAAAG AGAAGACTTC 
AAAGGAAAAA AGATTGAACT TAATGCAGAA ACTGTCAATC TCCGATCCCT GGGATTCAAC 
ACACAAATAC GCTCTGTTCA GGTTATTGGT GGCATATGGG TTACTTATGA ATATGGCAGT 
TACAGAGGGC GACAGTTCCT ATTGTCACCT GCAGAAGTAC CTAATTGGTA TGAATTCAGT 
GGCTGTCGCC AAATAGGTTC TCTACGACCT TTTGTTCAGA AGCGAATTTA TTTCAGACTT 
CGAAACAAAG CAACAGGGTT ATTCATGTCA ACCAATGGAA ACTTAGAGGA TCTGAAGCTT 
CTGAGGATAC AGGTCATGGA GGATGTCGGG GCCGATGATC AGATTTGGAT CTATCAAGAA 
GGATGTATCA AATGCAGGAT AGCAGAAGAC TGCTGCCTGA CGATTGTGGG CAGCCTGGTA 
ACATCTGGCT CCAAGCTAGG CCTGGCCCTG GACCAGAATG CTGACAGCCA GTTCTGGAGC 
TTGAAGTCCG ATGGCAGGAT TTACAGCAAG TTGAAGCCAA ATTTAGTTTT AGACATTAAA 
GGGGGCACAC AGTATGATCA AAATCACATT ATCCTCAACA CTGTCAGCAA AGAGAAGTTT 
ACACAAGTGT GGGAAGCCAT GGTCCTATAT ACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CRYBG1 ( NM_001624.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001615.2 Homo sapiens crystallin beta-gamma domain containing 1 (CRYBG1), transcript variant 2, mRNA.

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CloneID	OHu56219
Clone ID Related Accession (Same CDS sequence)	NM_001624.4 , NM_001624.3
Accession Version	NM_001624.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5172bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-02
Organism	Homo sapiens(human)
Product	beta/gamma crystallin domain-containing protein 1 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK056705.1, AL359292.12, U83115.1 and AL390074.17. On Dec 4, 2014 this sequence version replaced NM_001624.2. Transcript Variant: This variant (2) represents use of an alternate promoter and therefore differs in the 5' UTR and 5' coding region, compared to variant 1. These differences cause translation initiation at a downstream start codon and result in an isoform (2) with a shorter N-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U83115.1, SRR1803616.11797.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGAAGA GGTCCAGCGG CCGTAGGTCG GGGAGGCGGA GGGGGTCGCA GAAATCCACC 
GACTCCCCCG GCGCGGACGC CGAGCTCCCT GAGAGCGCTG CCAGGGACGA CGCGGTGTTC 
GACGACGAGG TGGCGCCAAA CGCGGCCAGC GATAACGCCT CGGCGGAAAA GAAAGTGAAA 
TCTCCGCGGG CAGCCCTCGA CGGGGGCGTT GCCTCCGCTG CGAGCCCAGA GTCCAAGCCC 
AGCCCCGGTA CCAAAGGGCA GCTCCGAGGG GAGTCGGACC GGAGCAAACA GCCACCCCCG 
GCTTCGTCCC CCACGAAGAG GAAGGGCAGG AGCCGTGCCC TCGAGGCCGT GCCCGCCCCG 
CCCGCCAGCG GCCCCCGGGC TCCCGCCAAG GAGTCCCCAC CCAAGAGGGT GCCCGATCCC 
AGCCCAGTCA CCAAGGGCAC TGCGGCCGAG AGCGGGGAGG AGGCGGCGCG GGCCATCCCC 
CGCGAGCTCC CGGTCAAGAG CAGCTCGCTG CTGCCGGAGA TCAAGCCCGA GCACAAGAGG 
GGCCCGCTCC CCAACCACTT CAACGGCCGG GCAGAGGGAG GTCGAAGCAG AGAGCTGGGC 
AGAGCGGCCG GAGCGCCTGG AGCTTCTGAC GCCGACGGCT TGAAGCCCAG GAACCATTTC 
GGCGTGGGCA GGTCGACAGT GACCACTAAA GTGACCCTCC CTGCCAAGCC CAAACATGTG 
GAACTAAATC TTAAAACCCC TAAGAATCTT GACAGTTTGG GAAATGAGCA CAATCCATTT 
AGCCAGCCAG TTCACAAAGG CAACACTGCC ACCAAAATCT CCTTATTTGA AAACAAACGG 
ACAAACAGTA GCCCAAGACA CACTGACATT CGAGGCCAAA GGAATACTCC TGCCTCTAGT 
AAAACGTTTG TTGGGAGGGC AAAGCTGAAT TTAGCCAAAA AAGCCAAAGA AATGGAGCAA 
CCTGAAAAGA AAGTAATGCC AAACAGTCCC CAGAATGGTG TGCTGGTTAA GGAAACTGCT 
ATAGAAACCA AAGTTACCGT CTCGGAAGAA GAGATTCTGC CAGCAACCAG AGGAATGAAT 
GGAGACTCTT CTGAGAATCA AGCTCTTGGT CCTCAGCCTA ACCAAGATGA TAAAGCAGAT 
GTACAAACAG ATGCTGGCTG CCTTTCAGAA CCAGTGGCTT CTGCTCTGAT TCCTGTCAAG 
GATCATAAGC TCTTAGAGAA GGAGGACTCA GAGGCTGCAG ACAGCAAAAG CCTTGTACTT 
GAAAATGTAA CCGATACAGC ACAAGACATC CCCACCACTG TGGATACCAA AGATTTACCT 
CCAACGGCCA TGCCAAAGCC ACAGCATACA TTTTCTGACT CACAGTCCCC TGCTGAGTCA 
TCTCCTGGGC CTTCTCTTTC ACTGTCTGCA CCCGCTCCTG GGGATGTTCC CAAAGACACA 
TGTGTTCAAT CACCCATAAG CAGTTTCCCA TGCACTGATC TAAAAGTGTC AGAAAACCAT 
AAAGGATGTG TTTTGCCTGT GTCTCGTCAG AACAATGAGA AAATGCCACT TTTAGAACTT 
GGAGGAGAAA CAACCCCTCC TTTGTCCACA GAGCGTAGTC CAGAAGCTGT GGGAAGTGAG 
TGTCCATCCA GAGTCCTCGT CCAGGTCAGG TCCTTCGTGC TCCCCGTGGA GAGCACCCAG 
GATGTGAGCT CCCAGGTCAT CCCAGAGAGC TCTGAAGTTA GAGAAGTGCA GTTGCCAACT 
TGTCACAGTA ATGAACCTGA AGTGGTTTCC GTTGCAAGTT GTGCTCCCCC ACAAGAGGAA 
GTACTGGGCA ATGAACACTC TCATTGCACA GCAGAGCTCG CGGCAAAATC TGGCCCACAA 
GTCATACCGC CAGCATCAGA GAAAACTCTG CCTATTCAGG CTCAAAGTCA GGGCAGCAGA 
ACACCCCTGA TGGCTGAATC CAGTCCCACC AACTCTCCCA GCAGCGGAAA TCACTTAGCC 
ACTCCTCAAA GGCCAGATCA GACTGTTACA AATGGCCAGG ATAGCCCTGC CAGCCTTTTG 
AACATTTCTG CTGGTAGTGA TGATAGTGTA TTTGATTCTT CTTCTGATAT GGAAAAATTC 
ACTGAAATTA TAAAACAGAT GGATAGCGCA GTTTGTATGC CCATGAAAAG AAAGAAGGCC 
AGGATGCCAA ACTCTCCTGC TCCTCACTTT GCCATGCCTC CTATTCACGA AGACCATTTA 
GAAAAGGTGT TTGATCCCAA AGTGTTTACC TTTGGTTTGG GGAAGAAGAA GGAAAGTCAG 
CCAGAAATGT CACCGGCTTT ACATTTGATG CAGAACCTTG ACACAAAATC CAAACTGAGA 
CCCAAACGTG CATCTGCTGA ACAGAGCGTC CTCTTCAAGT CCCTGCACAC CAACACTAAT 
GGGAACAGTG AGCCTCTGGT GATGCCGGAA ATCAATGACA AAGAGAACAG GGACGTCACA 
AATGGTGGCA TTAAGAGATC GAGACTAGAA AAAAGTGCAC TTTTCTCAAG CTTGTTATCT 
TCTTTACCAC AAGACAAAAT CTTTTCTCCT TCTGTGACAT CAGTCAACAC TATGACCACG 
GCTTTCAGTA CTTCTCAGAA CGGTTCCCTA TCTCAGTCTT CAGTGTCACA GCCCACGACT 
GAGGGTGCCC CGCCCTGTGG TTTGAACAAA GAACAGTCAA ATCTTCTGCC CGACAACTCC 
TTAAAGGTCT TCAATTTCAA CTCGTCAAGT ACATCACACT CCAGTTTGAA AAGTCCAAGC 
CACATGGAAA AATACCCGCA AAAAGAGAAA ACCAAAGAAG ATCTGGATTC ACGAAGCAAC 
CTACACTTGC CAGAAACTAA ATTTTCTGAA TTGTCAAAAC TGAAGAATGA TGATATGGAA 
AAGGCTAATC ATATTGAAAG TGTTATTAAA TCAAACTTGC CAAACTGTGC AAACAGTGAC 
ACCGACTTCA TGGGTCTTTT CAAATCAAGC CGGTATGACC CAAGCATTTC TTTTTCTGGA 
ATGTCATTAT CAGACACAAT GACACTTAGA GGAAGTGTCC AAAATAAACT CAATCCCCGA 
CCTGGAAAGG TAGTGATATA TAGTGAACCC GACGTCTCTG AGAAGTGCAT TGAAGTTTTC 
AGTGACATTC AGGATTGCAG TTCTTGGAGC CTCTCTCCAG TGATACTCAT AAAAGTTGTT 
AGAGGATGTT GGATTTTGTA TGAGCAACCA AATTTTGAAG GGCACTCCAT CCCCTTAGAA 
GAAGGAGAAT TGGAACTCTC TGGTCTCTGG GGTATAGAAG ACATTTTGGA AAGGCACGAA 
GAAGCAGAGT CTGATAAGCC AGTGGTGATT GGTTCCATCA GACATGTGGT TCAGGATTAC 
AGAGTTAGTC ACATTGACTT ATTTACTGAA CCAGAAGGGT TAGGAATCCT AAGTTCCTAC 
TTTGATGATA CTGAAGAAAT GCAGGGATTT GGTGTAATGC AGAAGACTTG TTCCATGAAA 
GTACATTGGG GCACGTGGCT GATTTATGAA GAACCTGGAT TTCAGGGTGT TCCTTTCATC 
CTGGAACCTG GTGAATACCC TGACTTGTCC TTCTGGGATA CAGAAGAAGC GTACATTGGA 
TCCATGCGGC CTCTGAAAAT GGGTGGCCGT AAAGTTGAAT TCCCTACAGA TCCAAAGGTA 
GTTGTTTATG AAAAGCCTTT CTTTGAAGGA AAATGTGTGG AACTAGAAAC AGGAATGTGT 
AGTTTTGTCA TGGAGGGAGG TGAAACAGAA GAGGCGACTG GAGACGATCA TTTGCCGTTT 
ACGTCAGTGG GGTCTATGAA AGTTCTAAGA GGCATTTGGG TTGCATATGA GAAACCTGGA 
TTTACCGGTC ATCAGTATTT GCTAGAAGAA GGAGAATACA GGGACTGGAA AGCCTGGGGA 
GGTTACAATG GAGAGCTTCA GTCTTTACGA CCTATATTAG GTGATTTTTC AAATGCTCAC 
ATGATAATGT ACAGTGAAAA AAACTTTGGA TCCAAAGGTT CCAGTATTGA TGTATTGGGA 
ATTGTTGCTA ATTTAAAGGA GACTGGATAT GGAGTGAAGA CACAGTCTAT TAATGTACTG 
AGTGGAGTAT GGGTAGCCTA TGAAAATCCT GACTTCACAG GAGAACAGTA TATACTGGAT 
AAAGGATTTT ATACCAGTTT TGAGGACTGG GGAGGCAAAA ATTGTAAGAT CTCTTCTGTT 
CAACCTATAT GTTTGGATTC TTTCACTGGC CCAAGGAGAC GAAATCAGAT TCACTTGTTT 
TCAGAACCAC AGTTTCAAGG TCACAGTCAA AGTTTTGAAG AAACAACAAG TCAAATTGAT 
GATTCATTTT CTACCAAGTC TTGCAGAGTT TCAGGAGGCA GCTGGGTTGT ATATGATGGA 
GAAAATTTCA CTGGTAATCA ATACGTGTTG GAAGAAGGCC ATTATCCTTG TCTGTCTGCA 
ATGGGATGCC CGCCTGGAGC AACTTTCAAG TCTCTTCGTT TTATAGATGT TGAATTTTCT 
GAACCAACAA TTATTCTCTT TGAAAGAGAA GACTTCAAAG GAAAAAAGAT TGAACTTAAT 
GCAGAAACTG TCAATCTCCG ATCCCTGGGA TTCAACACAC AAATACGCTC TGTTCAGGTT 
ATTGGTGGCA TATGGGTTAC TTATGAATAT GGCAGTTACA GAGGGCGACA GTTCCTATTG 
TCACCTGCAG AAGTACCTAA TTGGTATGAA TTCAGTGGCT GTCGCCAAAT AGGTTCTCTA 
CGACCTTTTG TTCAGAAGCG AATTTATTTC AGACTTCGAA ACAAAGCAAC AGGGTTATTC 
ATGTCAACCA ATGGAAACTT AGAGGATCTG AAGCTTCTGA GGATACAGGT CATGGAGGAT 
GTCGGGGCCG ATGATCAGAT TTGGATCTAT CAAGAAGGAT GTATCAAATG CAGGATAGCA 
GAAGACTGCT GCCTGACGAT TGTGGGCAGC CTGGTAACAT CTGGCTCCAA GCTAGGCCTG 
GCCCTGGACC AGAATGCTGA CAGCCAGTTC TGGAGCTTGA AGTCCGATGG CAGGATTTAC 
AGCAAGTTGA AGCCAAATTT AGTTTTAGAC ATTAAAGGGG GCACACAGTA TGATCAAAAT 
CACATTATCC TCAACACTGT CAGCAAAGAG AAGTTTACAC AAGTGTGGGA AGCCATGGTC 
CTATATACCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001615.2
CDS	488..5659
Translation	MEKRSSGRRSGRRRGSQKSTDSPGADAELPESAARDDAVFDDEVAPNAASDNASAEKKVKSPRAALDGGVASAASPESKPSPGTKGQLRGESDRSKQPPPASSPTKRKGRSRALEAVPAPPASGPRAPAKESPPKRVPDPSPVTKGTAAESGEEAARAIPRELPVKSSSLLPEIKPEHKRGPLPNHFNGRAEGGRSRELGRAAGAPGASDADGLKPRNHFGVGRSTVTTKVTLPAKPKHVELNLKTPKNLDSLGNEHNPFSQPVHKGNTATKISLFENKRTNSSPRHTDIRGQRNTPASSKTFVGRAKLNLAKKAKEMEQPEKKVMPNSPQNGVLVKETAIETKVTVSEEEILPATRGMNGDSSENQALGPQPNQDDKADVQTDAGCLSEPVASALIPVKDHKLLEKEDSEAADSKSLVLENVTDTAQDIPTTVDTKDLPPTAMPKPQHTFSDSQSPAESSPGPSLSLSAPAPGDVPKDTCVQSPISSFPCTDLKVSENHKGCVLPVSRQNNEKMPLLELGGETTPPLSTERSPEAVGSECPSRVLVQVRSFVLPVESTQDVSSQVIPESSEVREVQLPTCHSNEPEVVSVASCAPPQEEVLGNEHSHCTAELAAKSGPQVIPPASEKTLPIQAQSQGSRTPLMAESSPTNSPSSGNHLATPQRPDQTVTNGQDSPASLLNISAGSDDSVFDSSSDMEKFTEIIKQMDSAVCMPMKRKKARMPNSPAPHFAMPPIHEDHLEKVFDPKVFTFGLGKKKESQPEMSPALHLMQNLDTKSKLRPKRASAEQSVLFKSLHTNTNGNSEPLVMPEINDKENRDVTNGGIKRSRLEKSALFSSLLSSLPQDKIFSPSVTSVNTMTTAFSTSQNGSLSQSSVSQPTTEGAPPCGLNKEQSNLLPDNSLKVFNFNSSSTSHSSLKSPSHMEKYPQKEKTKEDLDSRSNLHLPETKFSELSKLKNDDMEKANHIESVIKSNLPNCANSDTDFMGLFKSSRYDPSISFSGMSLSDTMTLRGSVQNKLNPRPGKVVIYSEPDVSEKCIEVFSDIQDCSSWSLSPVILIKVVRGCWILYEQPNFEGHSIPLEEGELELSGLWGIEDILERHEEAESDKPVVIGSIRHVVQDYRVSHIDLFTEPEGLGILSSYFDDTEEMQGFGVMQKTCSMKVHWGTWLIYEEPGFQGVPFILEPGEYPDLSFWDTEEAYIGSMRPLKMGGRKVEFPTDPKVVVYEKPFFEGKCVELETGMCSFVMEGGETEEATGDDHLPFTSVGSMKVLRGIWVAYEKPGFTGHQYLLEEGEYRDWKAWGGYNGELQSLRPILGDFSNAHMIMYSEKNFGSKGSSIDVLGIVANLKETGYGVKTQSINVLSGVWVAYENPDFTGEQYILDKGFYTSFEDWGGKNCKISSVQPICLDSFTGPRRRNQIHLFSEPQFQGHSQSFEETTSQIDDSFSTKSCRVSGGSWVVYDGENFTGNQYVLEEGHYPCLSAMGCPPGATFKSLRFIDVEFSEPTIILFEREDFKGKKIELNAETVNLRSLGFNTQIRSVQVIGGIWVTYEYGSYRGRQFLLSPAEVPNWYEFSGCRQIGSLRPFVQKRIYFRLRNKATGLFMSTNGNLEDLKLLRIQVMEDVGADDQIWIYQEGCIKCRIAEDCCLTIVGSLVTSGSKLGLALDQNADSQFWSLKSDGRIYSKLKPNLVLDIKGGTQYDQNHIILNTVSKEKFTQVWEAMVLYT

Target ORF information:

RefSeq Version	NM_001624.3
Organism	Homo sapiens(human)
Definition	Homo sapiens crystallin beta-gamma domain containing 1 (CRYBG1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001624.3

ORF Insert Sequence:

ATGGAGAAGA GGTCCAGCGG CCGTAGGTCG GGGAGGCGGA GGGGGTCGCA GAAATCCACC 
GACTCCCCCG GCGCGGACGC CGAGCTCCCT GAGAGCGCTG CCAGGGACGA CGCGGTGTTC 
GACGACGAGG TGGCGCCAAA CGCGGCCAGC GATAACGCCT CGGCGGAAAA GAAAGTGAAA 
TCTCCGCGGG CAGCCCTCGA CGGGGGCGTT GCCTCCGCTG CGAGCCCAGA GTCCAAGCCC 
AGCCCCGGTA CCAAAGGGCA GCTCCGAGGG GAGTCGGACC GGAGCAAACA GCCACCCCCG 
GCTTCGTCCC CCACGAAGAG GAAGGGCAGG AGCCGTGCCC TCGAGGCCGT GCCCGCCCCG 
CCCGCCAGCG GCCCCCGGGC TCCCGCCAAG GAGTCCCCAC CCAAGAGGGT GCCCGATCCC 
AGCCCAGTCA CCAAGGGCAC TGCGGCCGAG AGCGGGGAGG AGGCGGCGCG GGCCATCCCC 
CGCGAGCTCC CGGTCAAGAG CAGCTCGCTG CTGCCGGAGA TCAAGCCCGA GCACAAGAGG 
GGCCCGCTCC CCAACCACTT CAACGGCCGG GCAGAGGGAG GTCGAAGCAG AGAGCTGGGC 
AGAGCGGCCG GAGCGCCTGG AGCTTCTGAC GCCGACGGCT TGAAGCCCAG GAACCATTTC 
GGCGTGGGCA GGTCGACAGT GACCACTAAA GTGACCCTCC CTGCCAAGCC CAAACATGTG 
GAACTAAATC TTAAAACCCC TAAGAATCTT GACAGTTTGG GAAATGAGCA CAATCCATTT 
AGCCAGCCAG TTCACAAAGG CAACACTGCC ACCAAAATCT CCTTATTTGA AAACAAACGG 
ACAAACAGTA GCCCAAGACA CACTGACATT CGAGGCCAAA GGAATACTCC TGCCTCTAGT 
AAAACGTTTG TTGGGAGGGC AAAGCTGAAT TTAGCCAAAA AAGCCAAAGA AATGGAGCAA 
CCTGAAAAGA AAGTAATGCC AAACAGTCCC CAGAATGGTG TGCTGGTTAA GGAAACTGCT 
ATAGAAACCA AAGTTACCGT CTCGGAAGAA GAGATTCTGC CAGCAACCAG AGGAATGAAT 
GGAGACTCTT CTGAGAATCA AGCTCTTGGT CCTCAGCCTA ACCAAGATGA TAAAGCAGAT 
GTACAAACAG ATGCTGGCTG CCTTTCAGAA CCAGTGGCTT CTGCTCTGAT TCCTGTCAAG 
GATCATAAGC TCTTAGAGAA GGAGGACTCA GAGGCTGCAG ACAGCAAAAG CCTTGTACTT 
GAAAATGTAA CCGATACAGC ACAAGACATC CCCACCACTG TGGATACCAA AGATTTACCT 
CCAACGGCCA TGCCAAAGCC ACAGCATACA TTTTCTGACT CACAGTCCCC TGCTGAGTCA 
TCTCCTGGGC CTTCTCTTTC ACTGTCTGCA CCCGCTCCTG GGGATGTTCC CAAAGACACA 
TGTGTTCAAT CACCCATAAG CAGTTTCCCA TGCACTGATC TAAAAGTGTC AGAAAACCAT 
AAAGGATGTG TTTTGCCTGT GTCTCGTCAG AACAATGAGA AAATGCCACT TTTAGAACTT 
GGAGGAGAAA CAACCCCTCC TTTGTCCACA GAGCGTAGTC CAGAAGCTGT GGGAAGTGAG 
TGTCCATCCA GAGTCCTCGT CCAGGTCAGG TCCTTCGTGC TCCCCGTGGA GAGCACCCAG 
GATGTGAGCT CCCAGGTCAT CCCAGAGAGC TCTGAAGTTA GAGAAGTGCA GTTGCCAACT 
TGTCACAGTA ATGAACCTGA AGTGGTTTCC GTTGCAAGTT GTGCTCCCCC ACAAGAGGAA 
GTACTGGGCA ATGAACACTC TCATTGCACA GCAGAGCTCG CGGCAAAATC TGGCCCACAA 
GTCATACCGC CAGCATCAGA GAAAACTCTG CCTATTCAGG CTCAAAGTCA GGGCAGCAGA 
ACACCCCTGA TGGCTGAATC CAGTCCCACC AACTCTCCCA GCAGCGGAAA TCACTTAGCC 
ACTCCTCAAA GGCCAGATCA GACTGTTACA AATGGCCAGG ATAGCCCTGC CAGCCTTTTG 
AACATTTCTG CTGGTAGTGA TGATAGTGTA TTTGATTCTT CTTCTGATAT GGAAAAATTC 
ACTGAAATTA TAAAACAGAT GGATAGCGCA GTTTGTATGC CCATGAAAAG AAAGAAGGCC 
AGGATGCCAA ACTCTCCTGC TCCTCACTTT GCCATGCCTC CTATTCACGA AGACCATTTA 
GAAAAGGTGT TTGATCCCAA AGTGTTTACC TTTGGTTTGG GGAAGAAGAA GGAAAGTCAG 
CCAGAAATGT CACCGGCTTT ACATTTGATG CAGAACCTTG ACACAAAATC CAAACTGAGA 
CCCAAACGTG CATCTGCTGA ACAGAGCGTC CTCTTCAAGT CCCTGCACAC CAACACTAAT 
GGGAACAGTG AGCCTCTGGT GATGCCGGAA ATCAATGACA AAGAGAACAG GGACGTCACA 
AATGGTGGCA TTAAGAGATC GAGACTAGAA AAAAGTGCAC TTTTCTCAAG CTTGTTATCT 
TCTTTACCAC AAGACAAAAT CTTTTCTCCT TCTGTGACAT CAGTCAACAC TATGACCACG 
GCTTTCAGTA CTTCTCAGAA CGGTTCCCTA TCTCAGTCTT CAGTGTCACA GCCCACGACT 
GAGGGTGCCC CGCCCTGTGG TTTGAACAAA GAACAGTCAA ATCTTCTGCC CGACAACTCC 
TTAAAGGTCT TCAATTTCAA CTCGTCAAGT ACATCACACT CCAGTTTGAA AAGTCCAAGC 
CACATGGAAA AATACCCGCA AAAAGAGAAA ACCAAAGAAG ATCTGGATTC ACGAAGCAAC 
CTACACTTGC CAGAAACTAA ATTTTCTGAA TTGTCAAAAC TGAAGAATGA TGATATGGAA 
AAGGCTAATC ATATTGAAAG TGTTATTAAA TCAAACTTGC CAAACTGTGC AAACAGTGAC 
ACCGACTTCA TGGGTCTTTT CAAATCAAGC CGGTATGACC CAAGCATTTC TTTTTCTGGA 
ATGTCATTAT CAGACACAAT GACACTTAGA GGAAGTGTCC AAAATAAACT CAATCCCCGA 
CCTGGAAAGG TAGTGATATA TAGTGAACCC GACGTCTCTG AGAAGTGCAT TGAAGTTTTC 
AGTGACATTC AGGATTGCAG TTCTTGGAGC CTCTCTCCAG TGATACTCAT AAAAGTTGTT 
AGAGGATGTT GGATTTTGTA TGAGCAACCA AATTTTGAAG GGCACTCCAT CCCCTTAGAA 
GAAGGAGAAT TGGAACTCTC TGGTCTCTGG GGTATAGAAG ACATTTTGGA AAGGCACGAA 
GAAGCAGAGT CTGATAAGCC AGTGGTGATT GGTTCCATCA GACATGTGGT TCAGGATTAC 
AGAGTTAGTC ACATTGACTT ATTTACTGAA CCAGAAGGGT TAGGAATCCT AAGTTCCTAC 
TTTGATGATA CTGAAGAAAT GCAGGGATTT GGTGTAATGC AGAAGACTTG TTCCATGAAA 
GTACATTGGG GCACGTGGCT GATTTATGAA GAACCTGGAT TTCAGGGTGT TCCTTTCATC 
CTGGAACCTG GTGAATACCC TGACTTGTCC TTCTGGGATA CAGAAGAAGC GTACATTGGA 
TCCATGCGGC CTCTGAAAAT GGGTGGCCGT AAAGTTGAAT TCCCTACAGA TCCAAAGGTA 
GTTGTTTATG AAAAGCCTTT CTTTGAAGGA AAATGTGTGG AACTAGAAAC AGGAATGTGT 
AGTTTTGTCA TGGAGGGAGG TGAAACAGAA GAGGCGACTG GAGACGATCA TTTGCCGTTT 
ACGTCAGTGG GGTCTATGAA AGTTCTAAGA GGCATTTGGG TTGCATATGA GAAACCTGGA 
TTTACCGGTC ATCAGTATTT GCTAGAAGAA GGAGAATACA GGGACTGGAA AGCCTGGGGA 
GGTTACAATG GAGAGCTTCA GTCTTTACGA CCTATATTAG GTGATTTTTC AAATGCTCAC 
ATGATAATGT ACAGTGAAAA AAACTTTGGA TCCAAAGGTT CCAGTATTGA TGTATTGGGA 
ATTGTTGCTA ATTTAAAGGA GACTGGATAT GGAGTGAAGA CACAGTCTAT TAATGTACTG 
AGTGGAGTAT GGGTAGCCTA TGAAAATCCT GACTTCACAG GAGAACAGTA TATACTGGAT 
AAAGGATTTT ATACCAGTTT TGAGGACTGG GGAGGCAAAA ATTGTAAGAT CTCTTCTGTT 
CAACCTATAT GTTTGGATTC TTTCACTGGC CCAAGGAGAC GAAATCAGAT TCACTTGTTT 
TCAGAACCAC AGTTTCAAGG TCACAGTCAA AGTTTTGAAG AAACAACAAG TCAAATTGAT 
GATTCATTTT CTACCAAGTC TTGCAGAGTT TCAGGAGGCA GCTGGGTTGT ATATGATGGA 
GAAAATTTCA CTGGTAATCA ATACGTGTTG GAAGAAGGCC ATTATCCTTG TCTGTCTGCA 
ATGGGATGCC CGCCTGGAGC AACTTTCAAG TCTCTTCGTT TTATAGATGT TGAATTTTCT 
GAACCAACAA TTATTCTCTT TGAAAGAGAA GACTTCAAAG GAAAAAAGAT TGAACTTAAT 
GCAGAAACTG TCAATCTCCG ATCCCTGGGA TTCAACACAC AAATACGCTC TGTTCAGGTT 
ATTGGTGGCA TATGGGTTAC TTATGAATAT GGCAGTTACA GAGGGCGACA GTTCCTATTG 
TCACCTGCAG AAGTACCTAA TTGGTATGAA TTCAGTGGCT GTCGCCAAAT AGGTTCTCTA 
CGACCTTTTG TTCAGAAGCG AATTTATTTC AGACTTCGAA ACAAAGCAAC AGGGTTATTC 
ATGTCAACCA ATGGAAACTT AGAGGATCTG AAGCTTCTGA GGATACAGGT CATGGAGGAT 
GTCGGGGCCG ATGATCAGAT TTGGATCTAT CAAGAAGGAT GTATCAAATG CAGGATAGCA 
GAAGACTGCT GCCTGACGAT TGTGGGCAGC CTGGTAACAT CTGGCTCCAA GCTAGGCCTG 
GCCCTGGACC AGAATGCTGA CAGCCAGTTC TGGAGCTTGA AGTCCGATGG CAGGATTTAC 
AGCAAGTTGA AGCCAAATTT AGTTTTAGAC ATTAAAGGGG GCACACAGTA TGATCAAAAT 
CACATTATCC TCAACACTGT CAGCAAAGAG AAGTTTACAC AAGTGTGGGA AGCCATGGTC 
CTATATACCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CRYBG1 ( XM_005266839.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_005266896.1 Homo sapiens crystallin beta-gamma domain containing 1 (CRYBG1), transcript variant X1, mRNA.

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CloneID	OHu40642
Clone ID Related Accession (Same CDS sequence)	NM_001371242.1 , XM_005266839.3 , NM_001371242.2
Accession Version	XM_005266839.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6396bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-25
Organism	Homo sapiens(human)
Product	beta/gamma crystallin domain-containing protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005266839.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Homo sapiens Annotation Release 109 Annotation Version :: 109 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGCTGT CCCCGCCAGC CCAGGGCGAC CCCGGGGAGC CCAGCCCGTG CAGGCCCCCT 
AAGAAGCACA CCACCTTCCA CCTCTGGCGC TCCAAAAAGA AGCAGCAGCC GGCGCCGCCT 
GACTGTGGGG TGTTCGTTCC GCACCCGCTC CCGGCGCCTG CCGGAGAGGC CAGAGCTTTG 
GATGTAGTCG ATGGAAAATA TGTGGTTCGA GACTCCCAGG AATTTCCACT GCACTGTGGG 
GAATCCCAGT TCTTCCACAC CACCAGTGAG GCGCTTGGTT CCTTACTTCT AGAGTCTGGA 
ATATTTAAAA AGTCCAGAGC ACAACCTCCA GAAGACAACA GAAGGAAGCC AGTTTTGGGG 
AAACTTGGCA CTCTATTCAC TGCAGGAAGG AGAAGAAACA GTAGAAACGG GTTAGAGAGT 
CCCACCAGAT CAAATGCCAA ACCACTCTCT CCCAAAGATG TGGTAGCCTC TCCTAAGCTC 
CCAGAGAGAG AGAGTGAGAG GAGCAGATCT CAGAGCAGCC AACTGAAGCA AACGGACACA 
AGCGAGGAGG GCTCCCCGCG GGAGAATCCC CGAGAGGCAG AGGGCGAGCT CCCCGAGAGC 
GGTGGCCCCG CAGCCCCCCC TGACGCCGAG CTGTCACCTC GCTGGAGCAG CAGTGCAGCG 
GCTGTGGCTG TGCAGCAGTG CCATGAAAAT GATTCACCCC AATTAGAACC TCTGGAGGCA 
GAGGGAGAGC CTTTCCCAGA TGCCACCACC ACTGCCAAGC AGCTGCATTC CTCGCCGGGA 
AATTCCTCCA GGCAAGAGAA CGCAGAGACG CCCGCCCGCA GTCCGGGGGA GGACGCTTCA 
CCAGGTGCTG GCCACGAACA GGAGGCTTTC CTGGGTGTGA GGGGTGCGCC AGGGTCGCCC 
ACCCAGGAGC GGCCCGCGGG AGGACTAGGC GAGGCCCCTA ACGGAGCCCC CAGTGTGTGT 
GCCGAAGAAG GCTCCCTGGG GCCCCGCAAC GCCCGCAGCC AGCCCCCCAA GGGCGCGTCT 
GATTTGCCAG GTGAGCCTCC GGCCGAGGGC GCAGCGCACA CGGCCAGCTC CGCGCAGGCA 
GACTGCACAG CCCGCCCCAA GGGTCACGCC CACCCTGCTA AGGTGCTAAC TTTGGACATC 
TACTTGAGTA AGACTGAGGG GGCACAAGTG GACGAGCCGG TCGTGATTAC TCCCAGAGCG 
GAAGATTGCG GTGACTGGGA CGACATGGAG AAGAGGTCCA GCGGCCGTAG GTCGGGGAGG 
CGGAGGGGGT CGCAGAAATC CACCGACTCC CCCGGCGCGG ACGCCGAGCT CCCTGAGAGC 
GCTGCCAGGG ACGACGCGGT GTTCGACGAC GAGGTGGCGC CAAACGCGGC CAGCGATAAC 
GCCTCGGCGG AAAAGAAAGT GAAATCTCCG CGGGCAGCCC TCGACGGGGG CGTTGCCTCC 
GCTGCGAGCC CAGAGTCCAA GCCCAGCCCC GGTACCAAAG GGCAGCTCCG AGGGGAGTCG 
GACCGGAGCA AACAGCCACC CCCGGCTTCG TCCCCCACGA AGAGGAAGGG CAGGAGCCGT 
GCCCTCGAGG CCGTGCCCGC CCCGCCCGCC AGCGGCCCCC GGGCTCCCGC CAAGGAGTCC 
CCACCCAAGA GGGTGCCCGA TCCCAGCCCA GTCACCAAGG GCACTGCGGC CGAGAGCGGG 
GAGGAGGCGG CGCGGGCCAT CCCCCGCGAG CTCCCGGTCA AGAGCAGCTC GCTGCTGCCG 
GAGATCAAGC CCGAGCACAA GAGGGGCCCG CTCCCCAACC ACTTCAACGG CCGGGCAGAG 
GGAGGTCGAA GCAGAGAGCT GGGCAGAGCG GCCGGAGCGC CTGGAGCTTC TGACGCCGAC 
GGCTTGAAGC CCAGGAACCA TTTCGGCGTG GGCAGGTCGA CAGTGACCAC TAAAGTGACC 
CTCCCTGCCA AGCCCAAACA TGTGGAACTA AATCTTAAAA CCCCTAAGAA TCTTGACAGT 
TTGGGAAATG AGCACAATCC ATTTAGCCAG CCAGTTCACA AAGGCAACAC TGCCACCAAA 
ATCTCCTTAT TTGAAAACAA ACGGACAAAC AGTAGCCCAA GACACACTGA CATTCGAGGC 
CAAAGGAATA CTCCTGCCTC TAGTAAAACG TTTGTTGGGA GGGCAAAGCT GAATTTAGCC 
AAAAAAGCCA AAGAAATGGA GCAACCTGAA AAGAAAGTAA TGCCAAACAG TCCCCAGAAT 
GGTGTGCTGG TTAAGGAAAC TGCTATAGAA ACCAAAGTTA CCGTCTCGGA AGAAGAGATT 
CTGCCAGCAA CCAGAGGAAT GAATGGAGAC TCTTCTGAGA ATCAAGCTCT TGGTCCTCAG 
CCTAACCAAG ATGATAAAGC AGATGTACAA ACAGATGCTG GCTGCCTTTC AGAACCAGTG 
GCTTCTGCTC TGATTCCTGT CAAGGATCAT AAGCTCTTAG AGAAGGAGGA CTCAGAGGCT 
GCAGACAGCA AAAGCCTTGT ACTTGAAAAT GTAACCGATA CAGCACAAGA CATCCCCACC 
ACTGTGGATA CCAAAGATTT ACCTCCAACG GCCATGCCAA AGCCACAGCA TACATTTTCT 
GACTCACAGT CCCCTGCTGA GTCATCTCCT GGGCCTTCTC TTTCACTGTC TGCACCCGCT 
CCTGGGGATG TTCCCAAAGA CACATGTGTT CAATCACCCA TAAGCAGTTT CCCATGCACT 
GATCTAAAAG TGTCAGAAAA CCATAAAGGA TGTGTTTTGC CTGTGTCTCG TCAGAACAAT 
GAGAAAATGC CACTTTTAGA ACTTGGAGGA GAAACAACCC CTCCTTTGTC CACAGAGCGT 
AGTCCAGAAG CTGTGGGAAG TGAGTGTCCA TCCAGAGTCC TCGTCCAGGT CAGGTCCTTC 
GTGCTCCCCG TGGAGAGCAC CCAGGATGTG AGCTCCCAGG TCATCCCAGA GAGCTCTGAA 
GTTAGAGAAG TGCAGTTGCC AACTTGTCAC AGTAATGAAC CTGAAGTGGT TTCCGTTGCA 
AGTTGTGCTC CCCCACAAGA GGAAGTACTG GGCAATGAAC ACTCTCATTG CACAGCAGAG 
CTCGCGGCAA AATCTGGCCC ACAAGTCATA CCGCCAGCAT CAGAGAAAAC TCTGCCTATT 
CAGGCTCAAA GTCAGGGCAG CAGAACACCC CTGATGGCTG AATCCAGTCC CACCAACTCT 
CCCAGCAGCG GAAATCACTT AGCCACTCCT CAAAGGCCAG ATCAGACTGT TACAAATGGC 
CAGGATAGCC CTGCCAGCCT TTTGAACATT TCTGCTGGTA GTGATGATAG TGTATTTGAT 
TCTTCTTCTG ATATGGAAAA ATTCACTGAA ATTATAAAAC AGATGGATAG CGCAGTTTGT 
ATGCCCATGA AAAGAAAGAA GGCCAGGATG CCAAACTCTC CTGCTCCTCA CTTTGCCATG 
CCTCCTATTC ACGAAGACCA TTTAGAAAAG GTGTTTGATC CCAAAGTGTT TACCTTTGGT 
TTGGGGAAGA AGAAGGAAAG TCAGCCAGAA ATGTCACCGG CTTTACATTT GATGCAGAAC 
CTTGACACAA AATCCAAACT GAGACCCAAA CGTGCATCTG CTGAACAGAG CGTCCTCTTC 
AAGTCCCTGC ACACCAACAC TAATGGGAAC AGTGAGCCTC TGGTGATGCC GGAAATCAAT 
GACAAAGAGA ACAGGGACGT CACAAATGGT GGCATTAAGA GATCGAGACT AGAAAAAAGT 
GCACTTTTCT CAAGCTTGTT ATCTTCTTTA CCACAAGACA AAATCTTTTC TCCTTCTGTG 
ACATCAGTCA ACACTATGAC CACGGCTTTC AGTACTTCTC AGAACGGTTC CCTATCTCAG 
TCTTCAGTGT CACAGCCCAC GACTGAGGGT GCCCCGCCCT GTGGTTTGAA CAAAGAACAG 
TCAAATCTTC TGCCCGACAA CTCCTTAAAG GTCTTCAATT TCAACTCGTC AAGTACATCA 
CACTCCAGTT TGAAAAGTCC AAGCCACATG GAAAAATACC CGCAAAAAGA GAAAACCAAA 
GAAGATCTGG ATTCACGAAG CAACCTACAC TTGCCAGAAA CTAAATTTTC TGAATTGTCA 
AAACTGAAGA ATGATGATAT GGAAAAGGCT AATCATATTG AAAGTGTTAT TAAATCAAAC 
TTGCCAAACT GTGCAAACAG TGACACCGAC TTCATGGGTC TTTTCAAATC AAGCCGGTAT 
GACCCAAGCA TTTCTTTTTC TGGAATGTCA TTATCAGACA CAATGACACT TAGAGGAAGT 
GTCCAAAATA AACTCAATCC CCGACCTGGA AAGGTAGTGA TATATAGTGA ACCCGACGTC 
TCTGAGAAGT GCATTGAAGT TTTCAGTGAC ATTCAGGATT GCAGTTCTTG GAGCCTCTCT 
CCAGTGATAC TCATAAAAGT TGTTAGAGGA TGTTGGATTT TGTATGAGCA ACCAAATTTT 
GAAGGGCACT CCATCCCCTT AGAAGAAGGA GAATTGGAAC TCTCTGGTCT CTGGGGTATA 
GAAGACATTT TGGAAAGGCA CGAAGAAGCA GAGTCTGATA AGCCAGTGGT GATTGGTTCC 
ATCAGACATG TGGTTCAGGA TTACAGAGTT AGTCACATTG ACTTATTTAC TGAACCAGAA 
GGGTTAGGAA TCCTAAGTTC CTACTTTGAT GATACTGAAG AAATGCAGGG ATTTGGTGTA 
ATGCAGAAGA CTTGTTCCAT GAAAGTACAT TGGGGCACGT GGCTGATTTA TGAAGAACCT 
GGATTTCAGG GTGTTCCTTT CATCCTGGAA CCTGGTGAAT ACCCTGACTT GTCCTTCTGG 
GATACAGAAG AAGCGTACAT TGGATCCATG CGGCCTCTGA AAATGGGTGG CCGTAAAGTT 
GAATTCCCTA CAGATCCAAA GGTAGTTGTT TATGAAAAGC CTTTCTTTGA AGGAAAATGT 
GTGGAACTAG AAACAGGAAT GTGTAGTTTT GTCATGGAGG GAGGTGAAAC AGAAGAGGCG 
ACTGGAGACG ATCATTTGCC GTTTACGTCA GTGGGGTCTA TGAAAGTTCT AAGAGGCATT 
TGGGTTGCAT ATGAGAAACC TGGATTTACC GGTCATCAGT ATTTGCTAGA AGAAGGAGAA 
TACAGGGACT GGAAAGCCTG GGGAGGTTAC AATGGAGAGC TTCAGTCTTT ACGACCTATA 
TTAGGTGATT TTTCAAATGC TCACATGATA ATGTACAGTG AAAAAAACTT TGGATCCAAA 
GGTTCCAGTA TTGATGTATT GGGAATTGTT GCTAATTTAA AGGAGACTGG ATATGGAGTG 
AAGACACAGT CTATTAATGT ACTGAGTGGA GTATGGGTAG CCTATGAAAA TCCTGACTTC 
ACAGGAGAAC AGTATATACT GGATAAAGGA TTTTATACCA GTTTTGAGGA CTGGGGAGGC 
AAAAATTGTA AGATCTCTTC TGTTCAACCT ATATGTTTGG ATTCTTTCAC TGGCCCAAGG 
AGACGAAATC AGATTCACTT GTTTTCAGAA CCACAGTTTC AAGGTCACAG TCAAAGTTTT 
GAAGAAACAA CAAGTCAAAT TGATGATTCA TTTTCTACCA AGTCTTGCAG AGTTTCAGGA 
GGCAGCTGGG TTGTATATGA TGGAGAAAAT TTCACTGGTA ATCAATACGT GTTGGAAGAA 
GGCCATTATC CTTGTCTGTC TGCAATGGGA TGCCCGCCTG GAGCAACTTT CAAGTCTCTT 
CGTTTTATAG ATGTTGAATT TTCTGAACCA ACAATTATTC TCTTTGAAAG AGAAGACTTC 
AAAGGAAAAA AGATTGAACT TAATGCAGAA ACTGTCAATC TCCGATCCCT GGGATTCAAC 
ACACAAATAC GCTCTGTTCA GGTTATTGGT GGCATATGGG TTACTTATGA ATATGGCAGT 
TACAGAGGGC GACAGTTCCT ATTGTCACCT GCAGAAGTAC CTAATTGGTA TGAATTCAGT 
GGCTGTCGCC AAATAGGTTC TCTACGACCT TTTGTTCAGA AGCGAATTTA TTTCAGACTT 
CGAAACAAAG CAACAGGGTT ATTCATGTCA ACCAATGGAA ACTTAGAGGA TCTGAAGCTT 
CTGAGGATAC AGGTCATGGA GGATGTCGGG GCCGATGATC AGATTTGGAT CTATCAAGAA 
GGATGTATCA AATGCAGGAT AGCAGAAGAC TGCTGCCTGA CGATTGTGGG CAGCCTGGTA 
ACATCTGGCT CCAAGCTAGG CCTGGCCCTG GACCAGAATG CTGACAGCCA GTTCTGGAGC 
TTGAAGTCCG ATGGCAGGAT TTACAGCAAG TTGAAGCCAA ATTTAGTTTT AGACATTAAA 
GGGGGCACAC AGTATGATCA AAATCACATT ATCCTCAACA CTGTCAGCAA AGAGAAGTTT 
ACACAAGTGT GGGAAGCCAT GGTCCTATAT ACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005266896.1
CDS	102..6497
Translation	MPLSPPAQGDPGEPSPCRPPKKHTTFHLWRSKKKQQPAPPDCGVFVPHPLPAPAGEARALDVVDGKYVVRDSQEFPLHCGESQFFHTTSEALGSLLLESGIFKKSRAQPPEDNRRKPVLGKLGTLFTAGRRRNSRNGLESPTRSNAKPLSPKDVVASPKLPERESERSRSQSSQLKQTDTSEEGSPRENPREAEGELPESGGPAAPPDAELSPRWSSSAAAVAVQQCHENDSPQLEPLEAEGEPFPDATTTAKQLHSSPGNSSRQENAETPARSPGEDASPGAGHEQEAFLGVRGAPGSPTQERPAGGLGEAPNGAPSVCAEEGSLGPRNARSQPPKGASDLPGEPPAEGAAHTASSAQADCTARPKGHAHPAKVLTLDIYLSKTEGAQVDEPVVITPRAEDCGDWDDMEKRSSGRRSGRRRGSQKSTDSPGADAELPESAARDDAVFDDEVAPNAASDNASAEKKVKSPRAALDGGVASAASPESKPSPGTKGQLRGESDRSKQPPPASSPTKRKGRSRALEAVPAPPASGPRAPAKESPPKRVPDPSPVTKGTAAESGEEAARAIPRELPVKSSSLLPEIKPEHKRGPLPNHFNGRAEGGRSRELGRAAGAPGASDADGLKPRNHFGVGRSTVTTKVTLPAKPKHVELNLKTPKNLDSLGNEHNPFSQPVHKGNTATKISLFENKRTNSSPRHTDIRGQRNTPASSKTFVGRAKLNLAKKAKEMEQPEKKVMPNSPQNGVLVKETAIETKVTVSEEEILPATRGMNGDSSENQALGPQPNQDDKADVQTDAGCLSEPVASALIPVKDHKLLEKEDSEAADSKSLVLENVTDTAQDIPTTVDTKDLPPTAMPKPQHTFSDSQSPAESSPGPSLSLSAPAPGDVPKDTCVQSPISSFPCTDLKVSENHKGCVLPVSRQNNEKMPLLELGGETTPPLSTERSPEAVGSECPSRVLVQVRSFVLPVESTQDVSSQVIPESSEVREVQLPTCHSNEPEVVSVASCAPPQEEVLGNEHSHCTAELAAKSGPQVIPPASEKTLPIQAQSQGSRTPLMAESSPTNSPSSGNHLATPQRPDQTVTNGQDSPASLLNISAGSDDSVFDSSSDMEKFTEIIKQMDSAVCMPMKRKKARMPNSPAPHFAMPPIHEDHLEKVFDPKVFTFGLGKKKESQPEMSPALHLMQNLDTKSKLRPKRASAEQSVLFKSLHTNTNGNSEPLVMPEINDKENRDVTNGGIKRSRLEKSALFSSLLSSLPQDKIFSPSVTSVNTMTTAFSTSQNGSLSQSSVSQPTTEGAPPCGLNKEQSNLLPDNSLKVFNFNSSSTSHSSLKSPSHMEKYPQKEKTKEDLDSRSNLHLPETKFSELSKLKNDDMEKANHIESVIKSNLPNCANSDTDFMGLFKSSRYDPSISFSGMSLSDTMTLRGSVQNKLNPRPGKVVIYSEPDVSEKCIEVFSDIQDCSSWSLSPVILIKVVRGCWILYEQPNFEGHSIPLEEGELELSGLWGIEDILERHEEAESDKPVVIGSIRHVVQDYRVSHIDLFTEPEGLGILSSYFDDTEEMQGFGVMQKTCSMKVHWGTWLIYEEPGFQGVPFILEPGEYPDLSFWDTEEAYIGSMRPLKMGGRKVEFPTDPKVVVYEKPFFEGKCVELETGMCSFVMEGGETEEATGDDHLPFTSVGSMKVLRGIWVAYEKPGFTGHQYLLEEGEYRDWKAWGGYNGELQSLRPILGDFSNAHMIMYSEKNFGSKGSSIDVLGIVANLKETGYGVKTQSINVLSGVWVAYENPDFTGEQYILDKGFYTSFEDWGGKNCKISSVQPICLDSFTGPRRRNQIHLFSEPQFQGHSQSFEETTSQIDDSFSTKSCRVSGGSWVVYDGENFTGNQYVLEEGHYPCLSAMGCPPGATFKSLRFIDVEFSEPTIILFEREDFKGKKIELNAETVNLRSLGFNTQIRSVQVIGGIWVTYEYGSYRGRQFLLSPAEVPNWYEFSGCRQIGSLRPFVQKRIYFRLRNKATGLFMSTNGNLEDLKLLRIQVMEDVGADDQIWIYQEGCIKCRIAEDCCLTIVGSLVTSGSKLGLALDQNADSQFWSLKSDGRIYSKLKPNLVLDIKGGTQYDQNHIILNTVSKEKFTQVWEAMVLYT

Target ORF information:

RefSeq Version	XM_005266839.3
Organism	Homo sapiens(human)
Definition	Homo sapiens crystallin beta-gamma domain containing 1 (CRYBG1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005266839.3

ORF Insert Sequence:

ATGCCGCTGT CCCCGCCAGC CCAGGGCGAC CCCGGGGAGC CCAGCCCGTG CAGGCCCCCT 
AAGAAGCACA CCACCTTCCA CCTCTGGCGC TCCAAAAAGA AGCAGCAGCC GGCGCCGCCT 
GACTGTGGGG TGTTCGTTCC GCACCCGCTC CCGGCGCCTG CCGGAGAGGC CAGAGCTTTG 
GATGTAGTCG ATGGAAAATA TGTGGTTCGA GACTCCCAGG AATTTCCACT GCACTGTGGG 
GAATCCCAGT TCTTCCACAC CACCAGTGAG GCGCTTGGTT CCTTACTTCT AGAGTCTGGA 
ATATTTAAAA AGTCCAGAGC ACAACCTCCA GAAGACAACA GAAGGAAGCC AGTTTTGGGG 
AAACTTGGCA CTCTATTCAC TGCAGGAAGG AGAAGAAACA GTAGAAACGG GTTAGAGAGT 
CCCACCAGAT CAAATGCCAA ACCACTCTCT CCCAAAGATG TGGTAGCCTC TCCTAAGCTC 
CCAGAGAGAG AGAGTGAGAG GAGCAGATCT CAGAGCAGCC AACTGAAGCA AACGGACACA 
AGCGAGGAGG GCTCCCCGCG GGAGAATCCC CGAGAGGCAG AGGGCGAGCT CCCCGAGAGC 
GGTGGCCCCG CAGCCCCCCC TGACGCCGAG CTGTCACCTC GCTGGAGCAG CAGTGCAGCG 
GCTGTGGCTG TGCAGCAGTG CCATGAAAAT GATTCACCCC AATTAGAACC TCTGGAGGCA 
GAGGGAGAGC CTTTCCCAGA TGCCACCACC ACTGCCAAGC AGCTGCATTC CTCGCCGGGA 
AATTCCTCCA GGCAAGAGAA CGCAGAGACG CCCGCCCGCA GTCCGGGGGA GGACGCTTCA 
CCAGGTGCTG GCCACGAACA GGAGGCTTTC CTGGGTGTGA GGGGTGCGCC AGGGTCGCCC 
ACCCAGGAGC GGCCCGCGGG AGGACTAGGC GAGGCCCCTA ACGGAGCCCC CAGTGTGTGT 
GCCGAAGAAG GCTCCCTGGG GCCCCGCAAC GCCCGCAGCC AGCCCCCCAA GGGCGCGTCT 
GATTTGCCAG GTGAGCCTCC GGCCGAGGGC GCAGCGCACA CGGCCAGCTC CGCGCAGGCA 
GACTGCACAG CCCGCCCCAA GGGTCACGCC CACCCTGCTA AGGTGCTAAC TTTGGACATC 
TACTTGAGTA AGACTGAGGG GGCACAAGTG GACGAGCCGG TCGTGATTAC TCCCAGAGCG 
GAAGATTGCG GTGACTGGGA CGACATGGAG AAGAGGTCCA GCGGCCGTAG GTCGGGGAGG 
CGGAGGGGGT CGCAGAAATC CACCGACTCC CCCGGCGCGG ACGCCGAGCT CCCTGAGAGC 
GCTGCCAGGG ACGACGCGGT GTTCGACGAC GAGGTGGCGC CAAACGCGGC CAGCGATAAC 
GCCTCGGCGG AAAAGAAAGT GAAATCTCCG CGGGCAGCCC TCGACGGGGG CGTTGCCTCC 
GCTGCGAGCC CAGAGTCCAA GCCCAGCCCC GGTACCAAAG GGCAGCTCCG AGGGGAGTCG 
GACCGGAGCA AACAGCCACC CCCGGCTTCG TCCCCCACGA AGAGGAAGGG CAGGAGCCGT 
GCCCTCGAGG CCGTGCCCGC CCCGCCCGCC AGCGGCCCCC GGGCTCCCGC CAAGGAGTCC 
CCACCCAAGA GGGTGCCCGA TCCCAGCCCA GTCACCAAGG GCACTGCGGC CGAGAGCGGG 
GAGGAGGCGG CGCGGGCCAT CCCCCGCGAG CTCCCGGTCA AGAGCAGCTC GCTGCTGCCG 
GAGATCAAGC CCGAGCACAA GAGGGGCCCG CTCCCCAACC ACTTCAACGG CCGGGCAGAG 
GGAGGTCGAA GCAGAGAGCT GGGCAGAGCG GCCGGAGCGC CTGGAGCTTC TGACGCCGAC 
GGCTTGAAGC CCAGGAACCA TTTCGGCGTG GGCAGGTCGA CAGTGACCAC TAAAGTGACC 
CTCCCTGCCA AGCCCAAACA TGTGGAACTA AATCTTAAAA CCCCTAAGAA TCTTGACAGT 
TTGGGAAATG AGCACAATCC ATTTAGCCAG CCAGTTCACA AAGGCAACAC TGCCACCAAA 
ATCTCCTTAT TTGAAAACAA ACGGACAAAC AGTAGCCCAA GACACACTGA CATTCGAGGC 
CAAAGGAATA CTCCTGCCTC TAGTAAAACG TTTGTTGGGA GGGCAAAGCT GAATTTAGCC 
AAAAAAGCCA AAGAAATGGA GCAACCTGAA AAGAAAGTAA TGCCAAACAG TCCCCAGAAT 
GGTGTGCTGG TTAAGGAAAC TGCTATAGAA ACCAAAGTTA CCGTCTCGGA AGAAGAGATT 
CTGCCAGCAA CCAGAGGAAT GAATGGAGAC TCTTCTGAGA ATCAAGCTCT TGGTCCTCAG 
CCTAACCAAG ATGATAAAGC AGATGTACAA ACAGATGCTG GCTGCCTTTC AGAACCAGTG 
GCTTCTGCTC TGATTCCTGT CAAGGATCAT AAGCTCTTAG AGAAGGAGGA CTCAGAGGCT 
GCAGACAGCA AAAGCCTTGT ACTTGAAAAT GTAACCGATA CAGCACAAGA CATCCCCACC 
ACTGTGGATA CCAAAGATTT ACCTCCAACG GCCATGCCAA AGCCACAGCA TACATTTTCT 
GACTCACAGT CCCCTGCTGA GTCATCTCCT GGGCCTTCTC TTTCACTGTC TGCACCCGCT 
CCTGGGGATG TTCCCAAAGA CACATGTGTT CAATCACCCA TAAGCAGTTT CCCATGCACT 
GATCTAAAAG TGTCAGAAAA CCATAAAGGA TGTGTTTTGC CTGTGTCTCG TCAGAACAAT 
GAGAAAATGC CACTTTTAGA ACTTGGAGGA GAAACAACCC CTCCTTTGTC CACAGAGCGT 
AGTCCAGAAG CTGTGGGAAG TGAGTGTCCA TCCAGAGTCC TCGTCCAGGT CAGGTCCTTC 
GTGCTCCCCG TGGAGAGCAC CCAGGATGTG AGCTCCCAGG TCATCCCAGA GAGCTCTGAA 
GTTAGAGAAG TGCAGTTGCC AACTTGTCAC AGTAATGAAC CTGAAGTGGT TTCCGTTGCA 
AGTTGTGCTC CCCCACAAGA GGAAGTACTG GGCAATGAAC ACTCTCATTG CACAGCAGAG 
CTCGCGGCAA AATCTGGCCC ACAAGTCATA CCGCCAGCAT CAGAGAAAAC TCTGCCTATT 
CAGGCTCAAA GTCAGGGCAG CAGAACACCC CTGATGGCTG AATCCAGTCC CACCAACTCT 
CCCAGCAGCG GAAATCACTT AGCCACTCCT CAAAGGCCAG ATCAGACTGT TACAAATGGC 
CAGGATAGCC CTGCCAGCCT TTTGAACATT TCTGCTGGTA GTGATGATAG TGTATTTGAT 
TCTTCTTCTG ATATGGAAAA ATTCACTGAA ATTATAAAAC AGATGGATAG CGCAGTTTGT 
ATGCCCATGA AAAGAAAGAA GGCCAGGATG CCAAACTCTC CTGCTCCTCA CTTTGCCATG 
CCTCCTATTC ACGAAGACCA TTTAGAAAAG GTGTTTGATC CCAAAGTGTT TACCTTTGGT 
TTGGGGAAGA AGAAGGAAAG TCAGCCAGAA ATGTCACCGG CTTTACATTT GATGCAGAAC 
CTTGACACAA AATCCAAACT GAGACCCAAA CGTGCATCTG CTGAACAGAG CGTCCTCTTC 
AAGTCCCTGC ACACCAACAC TAATGGGAAC AGTGAGCCTC TGGTGATGCC GGAAATCAAT 
GACAAAGAGA ACAGGGACGT CACAAATGGT GGCATTAAGA GATCGAGACT AGAAAAAAGT 
GCACTTTTCT CAAGCTTGTT ATCTTCTTTA CCACAAGACA AAATCTTTTC TCCTTCTGTG 
ACATCAGTCA ACACTATGAC CACGGCTTTC AGTACTTCTC AGAACGGTTC CCTATCTCAG 
TCTTCAGTGT CACAGCCCAC GACTGAGGGT GCCCCGCCCT GTGGTTTGAA CAAAGAACAG 
TCAAATCTTC TGCCCGACAA CTCCTTAAAG GTCTTCAATT TCAACTCGTC AAGTACATCA 
CACTCCAGTT TGAAAAGTCC AAGCCACATG GAAAAATACC CGCAAAAAGA GAAAACCAAA 
GAAGATCTGG ATTCACGAAG CAACCTACAC TTGCCAGAAA CTAAATTTTC TGAATTGTCA 
AAACTGAAGA ATGATGATAT GGAAAAGGCT AATCATATTG AAAGTGTTAT TAAATCAAAC 
TTGCCAAACT GTGCAAACAG TGACACCGAC TTCATGGGTC TTTTCAAATC AAGCCGGTAT 
GACCCAAGCA TTTCTTTTTC TGGAATGTCA TTATCAGACA CAATGACACT TAGAGGAAGT 
GTCCAAAATA AACTCAATCC CCGACCTGGA AAGGTAGTGA TATATAGTGA ACCCGACGTC 
TCTGAGAAGT GCATTGAAGT TTTCAGTGAC ATTCAGGATT GCAGTTCTTG GAGCCTCTCT 
CCAGTGATAC TCATAAAAGT TGTTAGAGGA TGTTGGATTT TGTATGAGCA ACCAAATTTT 
GAAGGGCACT CCATCCCCTT AGAAGAAGGA GAATTGGAAC TCTCTGGTCT CTGGGGTATA 
GAAGACATTT TGGAAAGGCA CGAAGAAGCA GAGTCTGATA AGCCAGTGGT GATTGGTTCC 
ATCAGACATG TGGTTCAGGA TTACAGAGTT AGTCACATTG ACTTATTTAC TGAACCAGAA 
GGGTTAGGAA TCCTAAGTTC CTACTTTGAT GATACTGAAG AAATGCAGGG ATTTGGTGTA 
ATGCAGAAGA CTTGTTCCAT GAAAGTACAT TGGGGCACGT GGCTGATTTA TGAAGAACCT 
GGATTTCAGG GTGTTCCTTT CATCCTGGAA CCTGGTGAAT ACCCTGACTT GTCCTTCTGG 
GATACAGAAG AAGCGTACAT TGGATCCATG CGGCCTCTGA AAATGGGTGG CCGTAAAGTT 
GAATTCCCTA CAGATCCAAA GGTAGTTGTT TATGAAAAGC CTTTCTTTGA AGGAAAATGT 
GTGGAACTAG AAACAGGAAT GTGTAGTTTT GTCATGGAGG GAGGTGAAAC AGAAGAGGCG 
ACTGGAGACG ATCATTTGCC GTTTACGTCA GTGGGGTCTA TGAAAGTTCT AAGAGGCATT 
TGGGTTGCAT ATGAGAAACC TGGATTTACC GGTCATCAGT ATTTGCTAGA AGAAGGAGAA 
TACAGGGACT GGAAAGCCTG GGGAGGTTAC AATGGAGAGC TTCAGTCTTT ACGACCTATA 
TTAGGTGATT TTTCAAATGC TCACATGATA ATGTACAGTG AAAAAAACTT TGGATCCAAA 
GGTTCCAGTA TTGATGTATT GGGAATTGTT GCTAATTTAA AGGAGACTGG ATATGGAGTG 
AAGACACAGT CTATTAATGT ACTGAGTGGA GTATGGGTAG CCTATGAAAA TCCTGACTTC 
ACAGGAGAAC AGTATATACT GGATAAAGGA TTTTATACCA GTTTTGAGGA CTGGGGAGGC 
AAAAATTGTA AGATCTCTTC TGTTCAACCT ATATGTTTGG ATTCTTTCAC TGGCCCAAGG 
AGACGAAATC AGATTCACTT GTTTTCAGAA CCACAGTTTC AAGGTCACAG TCAAAGTTTT 
GAAGAAACAA CAAGTCAAAT TGATGATTCA TTTTCTACCA AGTCTTGCAG AGTTTCAGGA 
GGCAGCTGGG TTGTATATGA TGGAGAAAAT TTCACTGGTA ATCAATACGT GTTGGAAGAA 
GGCCATTATC CTTGTCTGTC TGCAATGGGA TGCCCGCCTG GAGCAACTTT CAAGTCTCTT 
CGTTTTATAG ATGTTGAATT TTCTGAACCA ACAATTATTC TCTTTGAAAG AGAAGACTTC 
AAAGGAAAAA AGATTGAACT TAATGCAGAA ACTGTCAATC TCCGATCCCT GGGATTCAAC 
ACACAAATAC GCTCTGTTCA GGTTATTGGT GGCATATGGG TTACTTATGA ATATGGCAGT 
TACAGAGGGC GACAGTTCCT ATTGTCACCT GCAGAAGTAC CTAATTGGTA TGAATTCAGT 
GGCTGTCGCC AAATAGGTTC TCTACGACCT TTTGTTCAGA AGCGAATTTA TTTCAGACTT 
CGAAACAAAG CAACAGGGTT ATTCATGTCA ACCAATGGAA ACTTAGAGGA TCTGAAGCTT 
CTGAGGATAC AGGTCATGGA GGATGTCGGG GCCGATGATC AGATTTGGAT CTATCAAGAA 
GGATGTATCA AATGCAGGAT AGCAGAAGAC TGCTGCCTGA CGATTGTGGG CAGCCTGGTA 
ACATCTGGCT CCAAGCTAGG CCTGGCCCTG GACCAGAATG CTGACAGCCA GTTCTGGAGC 
TTGAAGTCCG ATGGCAGGAT TTACAGCAAG TTGAAGCCAA ATTTAGTTTT AGACATTAAA 
GGGGGCACAC AGTATGATCA AAATCACATT ATCCTCAACA CTGTCAGCAA AGAGAAGTTT 
ACACAAGTGT GGGAAGCCAT GGTCCTATAT ACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CRYBG1 ( XM_017010333.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016865822.1 Homo sapiens crystallin beta-gamma domain containing 1 (CRYBG1), transcript variant X2, mRNA.

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CloneID	OHu96335
Clone ID Related Accession (Same CDS sequence)	XM_017010333.1
Accession Version	XM_017010333.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4635bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	beta/gamma crystallin domain-containing protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAAGA GGTCCAGCGG CCGTAGGTCG GGGAGGCGGA GGGGGTCGCA GAAATCCACC 
GACTCCCCCG GCGCGGACGC CGAGCTCCCT GAGAGCGCTG CCAGGGACGA CGCGGTGTTC 
GACGACGAGG TGGCGCCAAA CGCGGCCAGC GATAACGCCT CGGCGGAAAA GAAAGTGAAA 
TCTCCGCGGG CAGCCCTCGA CGGGGGCGTT GCCTCCGCTG CGAGCCCAGA GTCCAAGCCC 
AGCCCCGGTA CCAAAGGGCA GCTCCGAGGG GAGTCGGACC GGAGCAAACA GCCACCCCCG 
GCTTCGTCCC CCACGAAGAG GAAGGGCAGG AGCCGTGCCC TCGAGGCCGT GCCCGCCCCG 
CCCGCCAGCG GCCCCCGGGC TCCCGCCAAG GAGTCCCCAC CCAAGAGGGT GCCCGATCCC 
AGCCCAGTCA CCAAGGGCAC TGCGGCCGAG AGCGGGGAGG AGGCGGCGCG GGCCATCCCC 
CGCGAGCTCC CGGTCAAGAG CAGCTCGCTG CTGCCGGAGA TCAAGCCCGA GCACAAGAGG 
GGCCCGCTCC CCAACCACTT CAACGGCCGG GCAGAGGGAG GTCGAAGCAG AGAGCTGGGC 
AGAGCGGCCG GAGCGCCTGG AGCTTCTGAC GCCGACGGCT TGAAGCCCAG GAACCATTTC 
GGCGTGGGCA GGTCGACAGT GACCACTAAA GTGACCCTCC CTGCCAAGCC CAAACATGTG 
GAACTAAATC TTAAAACCCC TAAGAATCTT GACAGTTTGG GAAATGAGCA CAATCCATTT 
AGCCAGCCAG TTCACAAAGG CAACACTGCC ACCAAAATCT CCTTATTTGA AAACAAACGG 
ACAAACAGTA GCCCAAGACA CACTGACATT CGAGGCCAAA GGAATACTCC TGCCTCTAGT 
AAAACGTTTG TTGGGAGGGC AAAGCTGAAT TTAGCCAAAA AAGCCAAAGA AATGGAGCAA 
CCTGAAAAGA AAGTAATGCC AAACAGTCCC CAGAATGGTG TGCTGGTTAA GGAAACTGCT 
ATAGAAACCA AAGTTACCGT CTCGGAAGAA GAGATTCTGC CAGCAACCAG AGGAATGAAT 
GGAGACTCTT CTGAGAATCA AGCTCTTGGT CCTCAGCCTA ACCAAGATGA TAAAGCAGAT 
GTACAAACAG ATGCTGGCTG CCTTTCAGAA CCAGTGGCTT CTGCTCTGAT TCCTGTCAAG 
GATCATAAGC TCTTAGAGAA GGAGGACTCA GAGGCTGCAG ACAGCAAAAG CCTTGTACTT 
GAAAATGTAA CCGATACAGC ACAAGACATC CCCACCACTG TGGATACCAA AGATTTACCT 
CCAACGGCCA TGCCAAAGCC ACAGCATACA TTTTCTGACT CACAGTCCCC TGCTGAGTCA 
TCTCCTGGGC CTTCTCTTTC ACTGTCTGCA CCCGCTCCTG GGGATGTTCC CAAAGACACA 
TGTGTTCAAT CACCCATAAG CAGTTTCCCA TGCACTGATC TAAAAGTGTC AGAAAACCAT 
AAAGGATGTG TTTTGCCTGT GTCTCGTCAG AACAATGAGA AAATGCCACT TTTAGAACTT 
GGAGGAGAAA CAACCCCTCC TTTGTCCACA GAGCGTAGTC CAGAAGCTGT GGGAAGTGAG 
TGTCCATCCA GAGTCCTCGT CCAGGTCAGG TCCTTCGTGC TCCCCGTGGA GAGCACCCAG 
GATGTGAGCT CCCAGGTCAT CCCAGAGAGC TCTGAAGTTA GAGAAGTGCA GTTGCCAACT 
TGTCACAGTA ATGAACCTGA AGTGGTTTCC GTTGCAAGTT GTGCTCCCCC ACAAGAGGAA 
GTACTGGGCA ATGAACACTC TCATTGCACA GCAGAGCTCG CGGCAAAATC TGGCCCACAA 
GTCATACCGC CAGCATCAGA GAAAACTCTG CCTATTCAGG CTCAAAGTCA GGGCAGCAGA 
ACACCCCTGA TGGCTGAATC CAGTCCCACC AACTCTCCCA GCAGCGGAAA TCACTTAGCC 
ACTCCTCAAA GGCCAGATCA GACTGTTACA AATGGCCAGG ATAGCCCTGC CAGCCTTTTG 
AACATTTCTG CTGGTAGTGA TGATAGTGTA TTTGATTCTT CTTCTGATAT GGAAAAATTC 
ACTGAAATTA TAAAACAGAT GGATAGCGCA GTTTGTATGC CCATGAAAAG AAAGAAGGCC 
AGGATGCCAA ACTCTCCTGC TCCTCACTTT GCCATGCCTC CTATTCACGA AGACCATTTA 
GAAAAGGTGT TTGATCCCAA AGTGTTTACC TTTGGTTTGG GGAAGAAGAA GGAAAGTCAG 
CCAGAAATGT CACCGGCTTT ACATTTGATG CAGAACCTTG ACACAAAATC CAAACTGAGA 
CCCAAACGTG CATCTGCTGA ACAGAGCGTC CTCTTCAAGT CCCTGCACAC CAACACTAAT 
GGGAACAGTG AGCCTCTGGT GATGCCGGAA ATCAATGACA AAGAGAACAG GGACGTCACA 
AATGGTGGCA TTAAGAGATC GAGACTAGAA AAAAGTGCAC TTTTCTCAAG CTTGTTATCT 
TCTTTACCAC AAGACAAAAT CTTTTCTCCT TCTGTGACAT CAGTCAACAC TATGACCACG 
GCTTTCAGTA CTTCTCAGAA CGGTTCCCTA TCTCAGTCTT CAGTGTCACA GCCCACGACT 
GAGGGTGCCC CGCCCTGTGG TTTGAACAAA GAACAGTCAA ATCTTCTGCC CGACAACTCC 
TTAAAGGTCT TCAATTTCAA CTCGTCAAGT ACATCACACT CCAGTTTGAA AAGTCCAAGC 
CACATGGAAA AATACCCGCA AAAAGAGAAA ACCAAAGAAG ATCTGGATTC ACGAAGCAAC 
CTACACTTGC CAGAAACTAA ATTTTCTGAA TTGTCAAAAC TGAAGAATGA TGATATGGAA 
AAGGCTAATC ATATTGAAAG TGTTATTAAA TCAAACTTGC CAAACTGTGC AAACAGTGAC 
ACCGACTTCA TGGGTCTTTT CAAATCAAGC CGGTATGACC CAAGCATTTC TTTTTCTGGA 
ATGTCATTAT CAGACACAAT GACACTTAGA GGAAGTGTCC AAAATAAACT CAATCCCCGA 
CCTGGAAAGG TAGTGATATA TAGTGAACCC GACGTCTCTG AGAAGTGCAT TGAAGTTTTC 
AGTGACATTC AGGATTGCAG TTCTTGGAGC CTCTCTCCAG TGATACTCAT AAAAGTTGTT 
AGAGGATGTT GGATTTTGTA TGAGCAACCA AATTTTGAAG GGCACTCCAT CCCCTTAGAA 
GAAGGAGAAT TGGAACTCTC TGGTCTCTGG GGTATAGAAG ACATTTTGGA AAGGCACGAA 
GAAGCAGAGT CTGATAAGCC AGTGGTGATT GGTTCCATCA GACATGTGGT TCAGGATTAC 
AGAGTTAGTC ACATTGACTT ATTTACTGAA CCAGAAGGGT TAGGAATCCT AAGTTCCTAC 
TTTGATGATA CTGAAGAAAT GCAGGGATTT GGTGTAATGC AGAAGACTTG TTCCATGAAA 
GTACATTGGG GCACGTGGCT GATTTATGAA GAACCTGGAT TTCAGGGTGT TCCTTTCATC 
CTGGAACCTG GTGAATACCC TGACTTGTCC TTCTGGGATA CAGAAGAAGC GTACATTGGA 
TCCATGCGGC CTCTGAAAAT GGGTGGCCGT AAAGTTGAAT TCCCTACAGA TCCAAAGGTA 
GTTGTTTATG AAAAGCCTTT CTTTGAAGGA AAATGTGTGG AACTAGAAAC AGGAATGTGT 
AGTTTTGTCA TGGAGGGAGG TGAAACAGAA GAGGCGACTG GAGACGATCA TTTGCCGTTT 
ACGTCAGTGG GGTCTATGAA AGTTCTAAGA GGCATTTGGG TTGCATATGA GAAACCTGGA 
TTTACCGGTC ATCAGTATTT GCTAGAAGAA GGAGAATACA GGGACTGGAA AGCCTGGGGA 
GGTTACAATG GAGAGCTTCA GTCTTTACGA CCTATATTAG GTGATTTTTC AAATGCTCAC 
ATGATAATGT ACAGTGAAAA AAACTTTGGA TCCAAAGGTT CCAGTATTGA TGTATTGGGA 
ATTGTTGCTA ATTTAAAGGA GACTGGATAT GGAGTGAAGA CACAGTCTAT TAATGTACTG 
AGTGGAGTAT GGGTAGCCTA TGAAAATCCT GACTTCACAG GAGAACAGTA TATACTGGAT 
AAAGGATTTT ATACCAGTTT TGAGGACTGG GGAGGCAAAA ATTGTAAGAT CTCTTCTGTT 
CAACCTATAT GTTTGGATTC TTTCACTGGC CCAAGGAGAC GAAATCAGAT TCACTTGTTT 
TCAGAACCAC AGTTTCAAGG TCACAGTCAA AGTTTTGAAG AAACAACAAG TCAAATTGAT 
GATTCATTTT CTACCAAGTC TTGCAGAGTT TCAGGAGGCA GCTGGGTTGT ATATGATGGA 
GAAAATTTCA CTGGTAATCA ATACGTGTTG GAAGAAGGCC ATTATCCTTG TCTGTCTGCA 
ATGGGATGCC CGCCTGGAGC AACTTTCAAG TCTCTTCGTT TTATAGATGT TGAATTTTCT 
GAACCAACAA TTATTCTCTT TGAAAGAGAA GACTTCAAAG GAAAAAAGAT TGAACTTAAT 
GCAGAAACTG TCAATCTCCG ATCCCTGGGA TTCAACACAC AAATACGCTC TGTTCAGGTT 
ATTGGTGGCA TATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016865822.1
CDS	713..5347
Translation	MEKRSSGRRSGRRRGSQKSTDSPGADAELPESAARDDAVFDDEVAPNAASDNASAEKKVKSPRAALDGGVASAASPESKPSPGTKGQLRGESDRSKQPPPASSPTKRKGRSRALEAVPAPPASGPRAPAKESPPKRVPDPSPVTKGTAAESGEEAARAIPRELPVKSSSLLPEIKPEHKRGPLPNHFNGRAEGGRSRELGRAAGAPGASDADGLKPRNHFGVGRSTVTTKVTLPAKPKHVELNLKTPKNLDSLGNEHNPFSQPVHKGNTATKISLFENKRTNSSPRHTDIRGQRNTPASSKTFVGRAKLNLAKKAKEMEQPEKKVMPNSPQNGVLVKETAIETKVTVSEEEILPATRGMNGDSSENQALGPQPNQDDKADVQTDAGCLSEPVASALIPVKDHKLLEKEDSEAADSKSLVLENVTDTAQDIPTTVDTKDLPPTAMPKPQHTFSDSQSPAESSPGPSLSLSAPAPGDVPKDTCVQSPISSFPCTDLKVSENHKGCVLPVSRQNNEKMPLLELGGETTPPLSTERSPEAVGSECPSRVLVQVRSFVLPVESTQDVSSQVIPESSEVREVQLPTCHSNEPEVVSVASCAPPQEEVLGNEHSHCTAELAAKSGPQVIPPASEKTLPIQAQSQGSRTPLMAESSPTNSPSSGNHLATPQRPDQTVTNGQDSPASLLNISAGSDDSVFDSSSDMEKFTEIIKQMDSAVCMPMKRKKARMPNSPAPHFAMPPIHEDHLEKVFDPKVFTFGLGKKKESQPEMSPALHLMQNLDTKSKLRPKRASAEQSVLFKSLHTNTNGNSEPLVMPEINDKENRDVTNGGIKRSRLEKSALFSSLLSSLPQDKIFSPSVTSVNTMTTAFSTSQNGSLSQSSVSQPTTEGAPPCGLNKEQSNLLPDNSLKVFNFNSSSTSHSSLKSPSHMEKYPQKEKTKEDLDSRSNLHLPETKFSELSKLKNDDMEKANHIESVIKSNLPNCANSDTDFMGLFKSSRYDPSISFSGMSLSDTMTLRGSVQNKLNPRPGKVVIYSEPDVSEKCIEVFSDIQDCSSWSLSPVILIKVVRGCWILYEQPNFEGHSIPLEEGELELSGLWGIEDILERHEEAESDKPVVIGSIRHVVQDYRVSHIDLFTEPEGLGILSSYFDDTEEMQGFGVMQKTCSMKVHWGTWLIYEEPGFQGVPFILEPGEYPDLSFWDTEEAYIGSMRPLKMGGRKVEFPTDPKVVVYEKPFFEGKCVELETGMCSFVMEGGETEEATGDDHLPFTSVGSMKVLRGIWVAYEKPGFTGHQYLLEEGEYRDWKAWGGYNGELQSLRPILGDFSNAHMIMYSEKNFGSKGSSIDVLGIVANLKETGYGVKTQSINVLSGVWVAYENPDFTGEQYILDKGFYTSFEDWGGKNCKISSVQPICLDSFTGPRRRNQIHLFSEPQFQGHSQSFEETTSQIDDSFSTKSCRVSGGSWVVYDGENFTGNQYVLEEGHYPCLSAMGCPPGATFKSLRFIDVEFSEPTIILFEREDFKGKKIELNAETVNLRSLGFNTQIRSVQVIGGI

Target ORF information:

RefSeq Version	XM_017010333.1
Organism	Homo sapiens(human)
Definition	Homo sapiens crystallin beta-gamma domain containing 1 (CRYBG1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017010333.1

ORF Insert Sequence:

ATGGAGAAGA GGTCCAGCGG CCGTAGGTCG GGGAGGCGGA GGGGGTCGCA GAAATCCACC 
GACTCCCCCG GCGCGGACGC CGAGCTCCCT GAGAGCGCTG CCAGGGACGA CGCGGTGTTC 
GACGACGAGG TGGCGCCAAA CGCGGCCAGC GATAACGCCT CGGCGGAAAA GAAAGTGAAA 
TCTCCGCGGG CAGCCCTCGA CGGGGGCGTT GCCTCCGCTG CGAGCCCAGA GTCCAAGCCC 
AGCCCCGGTA CCAAAGGGCA GCTCCGAGGG GAGTCGGACC GGAGCAAACA GCCACCCCCG 
GCTTCGTCCC CCACGAAGAG GAAGGGCAGG AGCCGTGCCC TCGAGGCCGT GCCCGCCCCG 
CCCGCCAGCG GCCCCCGGGC TCCCGCCAAG GAGTCCCCAC CCAAGAGGGT GCCCGATCCC 
AGCCCAGTCA CCAAGGGCAC TGCGGCCGAG AGCGGGGAGG AGGCGGCGCG GGCCATCCCC 
CGCGAGCTCC CGGTCAAGAG CAGCTCGCTG CTGCCGGAGA TCAAGCCCGA GCACAAGAGG 
GGCCCGCTCC CCAACCACTT CAACGGCCGG GCAGAGGGAG GTCGAAGCAG AGAGCTGGGC 
AGAGCGGCCG GAGCGCCTGG AGCTTCTGAC GCCGACGGCT TGAAGCCCAG GAACCATTTC 
GGCGTGGGCA GGTCGACAGT GACCACTAAA GTGACCCTCC CTGCCAAGCC CAAACATGTG 
GAACTAAATC TTAAAACCCC TAAGAATCTT GACAGTTTGG GAAATGAGCA CAATCCATTT 
AGCCAGCCAG TTCACAAAGG CAACACTGCC ACCAAAATCT CCTTATTTGA AAACAAACGG 
ACAAACAGTA GCCCAAGACA CACTGACATT CGAGGCCAAA GGAATACTCC TGCCTCTAGT 
AAAACGTTTG TTGGGAGGGC AAAGCTGAAT TTAGCCAAAA AAGCCAAAGA AATGGAGCAA 
CCTGAAAAGA AAGTAATGCC AAACAGTCCC CAGAATGGTG TGCTGGTTAA GGAAACTGCT 
ATAGAAACCA AAGTTACCGT CTCGGAAGAA GAGATTCTGC CAGCAACCAG AGGAATGAAT 
GGAGACTCTT CTGAGAATCA AGCTCTTGGT CCTCAGCCTA ACCAAGATGA TAAAGCAGAT 
GTACAAACAG ATGCTGGCTG CCTTTCAGAA CCAGTGGCTT CTGCTCTGAT TCCTGTCAAG 
GATCATAAGC TCTTAGAGAA GGAGGACTCA GAGGCTGCAG ACAGCAAAAG CCTTGTACTT 
GAAAATGTAA CCGATACAGC ACAAGACATC CCCACCACTG TGGATACCAA AGATTTACCT 
CCAACGGCCA TGCCAAAGCC ACAGCATACA TTTTCTGACT CACAGTCCCC TGCTGAGTCA 
TCTCCTGGGC CTTCTCTTTC ACTGTCTGCA CCCGCTCCTG GGGATGTTCC CAAAGACACA 
TGTGTTCAAT CACCCATAAG CAGTTTCCCA TGCACTGATC TAAAAGTGTC AGAAAACCAT 
AAAGGATGTG TTTTGCCTGT GTCTCGTCAG AACAATGAGA AAATGCCACT TTTAGAACTT 
GGAGGAGAAA CAACCCCTCC TTTGTCCACA GAGCGTAGTC CAGAAGCTGT GGGAAGTGAG 
TGTCCATCCA GAGTCCTCGT CCAGGTCAGG TCCTTCGTGC TCCCCGTGGA GAGCACCCAG 
GATGTGAGCT CCCAGGTCAT CCCAGAGAGC TCTGAAGTTA GAGAAGTGCA GTTGCCAACT 
TGTCACAGTA ATGAACCTGA AGTGGTTTCC GTTGCAAGTT GTGCTCCCCC ACAAGAGGAA 
GTACTGGGCA ATGAACACTC TCATTGCACA GCAGAGCTCG CGGCAAAATC TGGCCCACAA 
GTCATACCGC CAGCATCAGA GAAAACTCTG CCTATTCAGG CTCAAAGTCA GGGCAGCAGA 
ACACCCCTGA TGGCTGAATC CAGTCCCACC AACTCTCCCA GCAGCGGAAA TCACTTAGCC 
ACTCCTCAAA GGCCAGATCA GACTGTTACA AATGGCCAGG ATAGCCCTGC CAGCCTTTTG 
AACATTTCTG CTGGTAGTGA TGATAGTGTA TTTGATTCTT CTTCTGATAT GGAAAAATTC 
ACTGAAATTA TAAAACAGAT GGATAGCGCA GTTTGTATGC CCATGAAAAG AAAGAAGGCC 
AGGATGCCAA ACTCTCCTGC TCCTCACTTT GCCATGCCTC CTATTCACGA AGACCATTTA 
GAAAAGGTGT TTGATCCCAA AGTGTTTACC TTTGGTTTGG GGAAGAAGAA GGAAAGTCAG 
CCAGAAATGT CACCGGCTTT ACATTTGATG CAGAACCTTG ACACAAAATC CAAACTGAGA 
CCCAAACGTG CATCTGCTGA ACAGAGCGTC CTCTTCAAGT CCCTGCACAC CAACACTAAT 
GGGAACAGTG AGCCTCTGGT GATGCCGGAA ATCAATGACA AAGAGAACAG GGACGTCACA 
AATGGTGGCA TTAAGAGATC GAGACTAGAA AAAAGTGCAC TTTTCTCAAG CTTGTTATCT 
TCTTTACCAC AAGACAAAAT CTTTTCTCCT TCTGTGACAT CAGTCAACAC TATGACCACG 
GCTTTCAGTA CTTCTCAGAA CGGTTCCCTA TCTCAGTCTT CAGTGTCACA GCCCACGACT 
GAGGGTGCCC CGCCCTGTGG TTTGAACAAA GAACAGTCAA ATCTTCTGCC CGACAACTCC 
TTAAAGGTCT TCAATTTCAA CTCGTCAAGT ACATCACACT CCAGTTTGAA AAGTCCAAGC 
CACATGGAAA AATACCCGCA AAAAGAGAAA ACCAAAGAAG ATCTGGATTC ACGAAGCAAC 
CTACACTTGC CAGAAACTAA ATTTTCTGAA TTGTCAAAAC TGAAGAATGA TGATATGGAA 
AAGGCTAATC ATATTGAAAG TGTTATTAAA TCAAACTTGC CAAACTGTGC AAACAGTGAC 
ACCGACTTCA TGGGTCTTTT CAAATCAAGC CGGTATGACC CAAGCATTTC TTTTTCTGGA 
ATGTCATTAT CAGACACAAT GACACTTAGA GGAAGTGTCC AAAATAAACT CAATCCCCGA 
CCTGGAAAGG TAGTGATATA TAGTGAACCC GACGTCTCTG AGAAGTGCAT TGAAGTTTTC 
AGTGACATTC AGGATTGCAG TTCTTGGAGC CTCTCTCCAG TGATACTCAT AAAAGTTGTT 
AGAGGATGTT GGATTTTGTA TGAGCAACCA AATTTTGAAG GGCACTCCAT CCCCTTAGAA 
GAAGGAGAAT TGGAACTCTC TGGTCTCTGG GGTATAGAAG ACATTTTGGA AAGGCACGAA 
GAAGCAGAGT CTGATAAGCC AGTGGTGATT GGTTCCATCA GACATGTGGT TCAGGATTAC 
AGAGTTAGTC ACATTGACTT ATTTACTGAA CCAGAAGGGT TAGGAATCCT AAGTTCCTAC 
TTTGATGATA CTGAAGAAAT GCAGGGATTT GGTGTAATGC AGAAGACTTG TTCCATGAAA 
GTACATTGGG GCACGTGGCT GATTTATGAA GAACCTGGAT TTCAGGGTGT TCCTTTCATC 
CTGGAACCTG GTGAATACCC TGACTTGTCC TTCTGGGATA CAGAAGAAGC GTACATTGGA 
TCCATGCGGC CTCTGAAAAT GGGTGGCCGT AAAGTTGAAT TCCCTACAGA TCCAAAGGTA 
GTTGTTTATG AAAAGCCTTT CTTTGAAGGA AAATGTGTGG AACTAGAAAC AGGAATGTGT 
AGTTTTGTCA TGGAGGGAGG TGAAACAGAA GAGGCGACTG GAGACGATCA TTTGCCGTTT 
ACGTCAGTGG GGTCTATGAA AGTTCTAAGA GGCATTTGGG TTGCATATGA GAAACCTGGA 
TTTACCGGTC ATCAGTATTT GCTAGAAGAA GGAGAATACA GGGACTGGAA AGCCTGGGGA 
GGTTACAATG GAGAGCTTCA GTCTTTACGA CCTATATTAG GTGATTTTTC AAATGCTCAC 
ATGATAATGT ACAGTGAAAA AAACTTTGGA TCCAAAGGTT CCAGTATTGA TGTATTGGGA 
ATTGTTGCTA ATTTAAAGGA GACTGGATAT GGAGTGAAGA CACAGTCTAT TAATGTACTG 
AGTGGAGTAT GGGTAGCCTA TGAAAATCCT GACTTCACAG GAGAACAGTA TATACTGGAT 
AAAGGATTTT ATACCAGTTT TGAGGACTGG GGAGGCAAAA ATTGTAAGAT CTCTTCTGTT 
CAACCTATAT GTTTGGATTC TTTCACTGGC CCAAGGAGAC GAAATCAGAT TCACTTGTTT 
TCAGAACCAC AGTTTCAAGG TCACAGTCAA AGTTTTGAAG AAACAACAAG TCAAATTGAT 
GATTCATTTT CTACCAAGTC TTGCAGAGTT TCAGGAGGCA GCTGGGTTGT ATATGATGGA 
GAAAATTTCA CTGGTAATCA ATACGTGTTG GAAGAAGGCC ATTATCCTTG TCTGTCTGCA 
ATGGGATGCC CGCCTGGAGC AACTTTCAAG TCTCTTCGTT TTATAGATGT TGAATTTTCT 
GAACCAACAA TTATTCTCTT TGAAAGAGAA GACTTCAAAG GAAAAAAGAT TGAACTTAAT 
GCAGAAACTG TCAATCTCCG ATCCCTGGGA TTCAACACAC AAATACGCTC TGTTCAGGTT 
ATTGGTGGCA TATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Single-molecule Force Spectroscopy Reveals the Calcium Dependence of the Alternative Conformations in the Native State of a ??-Crystallin Protein.
The Journal of biological chemistry291(35)18263-75(2016 08)
Scholl ZN,Li Q,Yang W,Marszalek PE

DNMT1 and AIM1 Imprinting in human placenta revealed through a genome-wide screen for allele-specific DNA methylation.
BMC genomics14685(2013 Oct)
Das R,Lee YK,Strogantsev R,Jin S,Lim YC,Ng PY,Lin XM,Chng K,Yeo GSh,Ferguson-Smith AC,Ding C

AIM1 promoter hypermethylation as a predictor of decreased risk of recurrence following radical prostatectomy.
The Prostate72(10)1133-9(2012 Jul)
Rosenbaum E,Begum S,Brait M,Zahurak M,Maldonado L,Mangold LA,Eisenberger MA,Epstein JI,Partin AW,Sidransky D,Hoque MO

AIM1 and LINE-1 epigenetic aberrations in tumor and serum relate to melanoma progression and disease outcome.
The Journal of investigative dermatology132(6)1689-97(2012 Jun)
Hoshimoto S,Kuo CT,Chong KK,Takeshima TL,Takei Y,Li MW,Huang SK,Sim MS,Morton DL,Hoon DS

High-throughput transcriptomic and RNAi analysis identifies AIM1, ERGIC1, TMED3 and TPX2 as potential drug targets in prostate cancer.
PloS one7(6)e39801(2012)
Vainio P,Mpindi JP,Kohonen P,Fey V,Mirtti T,Alanen KA,Per?l? M,Kallioniemi O,Iljin K

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CRYBG1 cDNA ORF clone, Homo sapiens(human)

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Target ORF information:

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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