PIKFYVE cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	PIKFYVE
Entrez Gene ID	200576
Full Name	phosphoinositide kinase, FYVE-type zinc finger containing
Synonyms	CFD,FAB1,HEL37,PIP5K,PIP5K3,ZFYVE29
General protein information	Preferred Names phosphoinositide kinase, FYVE-type zinc finger containing Names 1-phosphatidylinositol 3-phosphate 5-kinase PIPkin-III epididymis luminal protein 37 phosphatidylinositol 3-phosphate 5-kinase type III phosphatidylinositol-3-phosphate/phosphatidylinositol 5-kinase, type III phosphoinositide kinase, FYVE finger containing type III PIP kinase zinc finger, FYVE domain containing 29
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 2 Map Location: 2q34
Summary	Phosphorylated derivatives of phosphatidylinositol (PtdIns) regulate cytoskeletal functions, membrane trafficking, and receptor signaling by recruiting protein complexes to cell- and endosomal-membranes. Humans have multiple PtdIns proteins that differ by the degree and position of phosphorylation of the inositol ring. This gene encodes an enzyme (PIKfyve; also known as phosphatidylinositol-3-phosphate 5-kinase type III or PIPKIII) that phosphorylates the D-5 position in PtdIns and phosphatidylinositol-3-phosphate (PtdIns3P) to make PtdIns5P and PtdIns(3,5)biphosphate. The D-5 position also can be phosphorylated by type I PtdIns4P-5-kinases (PIP5Ks) that are encoded by distinct genes and preferentially phosphorylate D-4 phosphorylated PtdIns. In contrast, PIKfyve preferentially phosphorylates D-3 phosphorylated PtdIns. In addition to being a lipid kinase, PIKfyve also has protein kinase activity. PIKfyve regulates endomembrane homeostasis and plays a role in the biogenesis of endosome carrier vesicles from early endosomes. Mutations in this gene cause corneal fleck dystrophy (CFD); an autosomal dominant disorder characterized by numerous small white flecks present in all layers of the corneal stroma. Histologically, these flecks appear to be keratocytes distended with lipid and mucopolysaccharide filled intracytoplasmic vacuoles. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, May 2010].
Disorder MIM:	609414
Disorder Html:	Corneal fleck dystrophy, 121850 (3)

mRNA and Protein(s)

mRNA	Protein	Name
XM_011510786.2	XP_011509088.1	1-phosphatidylinositol 3-phosphate 5-kinase isoform X10
XM_011510778.2	XP_011509080.1	1-phosphatidylinositol 3-phosphate 5-kinase isoform X1
XM_011510781.2	XP_011509083.1	1-phosphatidylinositol 3-phosphate 5-kinase isoform X3
XM_011510782.2	XP_011509084.1	1-phosphatidylinositol 3-phosphate 5-kinase isoform X5
XM_011510778.3	XP_011509080.1	1-phosphatidylinositol 3-phosphate 5-kinase isoform X1
XM_011510781.3	XP_011509083.1	1-phosphatidylinositol 3-phosphate 5-kinase isoform X3
XM_011510782.3	XP_011509084.1	1-phosphatidylinositol 3-phosphate 5-kinase isoform X5
XM_011510790.1	XP_011509092.1	1-phosphatidylinositol 3-phosphate 5-kinase isoform X16
NM_001178000.1	NP_001171471.1	1-phosphatidylinositol 3-phosphate 5-kinase isoform 4
XM_011510780.2	XP_011509082.1	1-phosphatidylinositol 3-phosphate 5-kinase isoform X2
XM_017003568.1	XP_016859057.1	1-phosphatidylinositol 3-phosphate 5-kinase isoform X4
XM_011510784.2	XP_011509086.1	1-phosphatidylinositol 3-phosphate 5-kinase isoform X7
XM_011510788.1	XP_011509090.1	1-phosphatidylinositol 3-phosphate 5-kinase isoform X12
XM_011510789.2	XP_011509091.1	1-phosphatidylinositol 3-phosphate 5-kinase isoform X13
XM_017003570.1	XP_016859059.1	1-phosphatidylinositol 3-phosphate 5-kinase isoform X14
XM_017003571.1	XP_016859060.1	1-phosphatidylinositol 3-phosphate 5-kinase isoform X15
XM_017003572.1	XP_016859061.1	1-phosphatidylinositol 3-phosphate 5-kinase isoform X16
XM_011510786.3	XP_011509088.1	1-phosphatidylinositol 3-phosphate 5-kinase isoform X10
XM_011510787.1	XP_011509089.1	1-phosphatidylinositol 3-phosphate 5-kinase isoform X11
XM_017003573.1	XP_016859062.1	1-phosphatidylinositol 3-phosphate 5-kinase isoform X16
XM_011510792.3	XP_011509094.1	1-phosphatidylinositol 3-phosphate 5-kinase isoform X17
NM_015040.4	NP_055855.2	1-phosphatidylinositol 3-phosphate 5-kinase isoform 2
XM_011510785.3	XP_011509087.1	1-phosphatidylinositol 3-phosphate 5-kinase isoform X8
XM_011510785.2	XP_011509087.1	1-phosphatidylinositol 3-phosphate 5-kinase isoform X8
NM_015040.3	NP_055855.2	1-phosphatidylinositol 3-phosphate 5-kinase isoform 2
XM_011510792.2	XP_011509094.1	1-phosphatidylinositol 3-phosphate 5-kinase isoform X17
XM_011510783.2	XP_011509085.1	1-phosphatidylinositol 3-phosphate 5-kinase isoform X6
XM_011510779.2	XP_011509081.1	1-phosphatidylinositol 3-phosphate 5-kinase isoform X1
XM_011510783.3	XP_011509085.1	1-phosphatidylinositol 3-phosphate 5-kinase isoform X6
XM_017003569.1	XP_016859058.1	1-phosphatidylinositol 3-phosphate 5-kinase isoform X9
XM_017003574.1	XP_016859063.1	1-phosphatidylinositol 3-phosphate 5-kinase isoform X16
NM_152671.3	NP_689884.1	1-phosphatidylinositol 3-phosphate 5-kinase isoform 3

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Pikfyve	NP_035216.2
Canis lupus familiaris (dog)	PIKFYVE	XP_005640650.1
Rattus norvegicus (Norway rat)	Pikfyve	XP_006245139.1
Gallus gallus (chicken)	PIKFYVE	XP_004942735.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005216	XP_314118.4
Drosophila melanogaster (fruit fly)	fab1	NP_995880.1
Homo sapiens (human)	PIKFYVE	NP_055855.2
Bos taurus (cattle)	PIKFYVE	NP_001192396.1
Pan troglodytes (chimpanzee)	PIKFYVE	XP_516057.2
	PIKFYVE	XP_001108540.1
Danio rerio (zebrafish)	pikfyve	NP_001120777.1

Gene Ontology
Bibliography

Related articles in PubMed

The Lipid Kinase PIKfyve Coordinates the Neutrophil Immune Response through the Activation of the Rac GTPase.
Dayam RM, Sun CX, Choy CH, Mancuso G, Glogauer M, Botelho RJ
Journal of immunology (Baltimore, Md. : 1950)199(6)2096-2105(2017 Sep)

PIKfyve inhibition increases exosome release and induces secretory autophagy.
Hessvik NP, ?verbye A, Brech A, Torgersen ML, Jakobsen IS, Sandvig K, Llorente A
Cellular and molecular life sciences : CMLS73(24)4717-4737(2016 Dec)

PIKfyve Regulates Vacuole Maturation and Nutrient Recovery following Engulfment.
Krishna S, Palm W, Lee Y, Yang W, Bandyopadhyay U, Xu H, Florey O, Thompson CB, Overholtzer M
Developmental cell38(5)536-47(2016 09)

A cell-permeable tool for analysing APP intracellular domain function and manipulation of PIKfyve activity.
Guscott B, Balklava Z, Safrany ST, Wassmer T
Bioscience reports36(2)(2016)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

this study shows that PIKfyve coordinates the neutrophil immune response through the activation of the Rac GTPase
Title: The Lipid Kinase PIKfyve Coordinates the Neutrophil Immune Response through the Activation of the Rac GTPase.

in PC-3 cells inhibition of PIKfyve by apilimod or depletion by siRNA increased the secretion of the exosomal fraction.
Title: PIKfyve inhibition increases exosome release and induces secretory autophagy.

Here we identify the lipid kinase PIKfyve as a regulator of an alternative pathway that distributes engulfed contents in support of intracellular macromolecular synthesis during macropinocytosis, entosis, and phagocytosis. We find that PIKfyve regulates vacuole size in part through its downstream effector, the cationic transporter TRPML1
Title: PIKfyve Regulates Vacuole Maturation and Nutrient Recovery following Engulfment.

A cell-permeable tool for analysing APP intracellular domain function and manipulation of PIKfyve activity.
Title: A cell-permeable tool for analysing APP intracellular domain function and manipulation of PIKfyve activity.

data identify a novel role of the ArPIKfyve-Sac3 complex in the mechanisms controlling aggregate formation of Sph1 and suggest that Sac3 protein deficiency or overproduction may facilitate aggregation of aggregation-prone proteins
Title: The Protein Complex of Neurodegeneration-related Phosphoinositide Phosphatase Sac3 and ArPIKfyve Binds the Lewy Body-associated Synphilin-1, Preventing Its Aggregation.

The following PIKFYVE gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PIKFYVE cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu13271	NM_152671.3 Latest version!	Homo sapiens phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu18641	NM_015040.4 Latest version!	Homo sapiens phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$659.50-$923.30 $1319.00
OHu09478	NM_001178000.1 Latest version!	Homo sapiens phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$321.30 $459.00
OHu89145	XM_017003570.1 Latest version!	Homo sapiens phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu89146	XM_017003571.1 Latest version!	Homo sapiens phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu77671	XM_011510789.2 Latest version!	Homo sapiens phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu77672	XM_017003573.1 Latest version!	Homo sapiens phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu77672	XM_017003574.1 Latest version!	Homo sapiens phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X20, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu77672	XM_017003572.1 Latest version!	Homo sapiens phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu77672	XM_011510790.1 Latest version!	Homo sapiens phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu77673	XM_011510792.3 Latest version!	Homo sapiens phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X21, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
OHu89143	XM_017003568.1 Latest version!	Homo sapiens phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OHu89144	XM_017003569.1 Latest version!	Homo sapiens phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OHu77661	XM_011510778.3 Latest version!	Homo sapiens phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OHu77661	XM_011510779.2 Latest version!	Homo sapiens phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OHu77662	XM_011510780.2 Latest version!	Homo sapiens phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OHu77663	XM_011510781.3 Latest version!	Homo sapiens phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OHu77664	XM_011510782.3 Latest version!	Homo sapiens phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OHu77665	XM_011510783.3 Latest version!	Homo sapiens phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OHu77666	XM_011510784.2 Latest version!	Homo sapiens phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OHu77667	XM_011510785.3 Latest version!	Homo sapiens phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OHu77668	XM_011510786.3 Latest version!	Homo sapiens phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OHu77669	XM_011510787.1 Latest version!	Homo sapiens phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OHu77670	XM_011510788.1 Latest version!	Homo sapiens phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
View Old Accession Versions >>
OHu77661	XM_011510778.2	Homo sapiens phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OHu77663	XM_011510781.2	Homo sapiens phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OHu77664	XM_011510782.2	Homo sapiens phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OHu77668	XM_011510786.2	Homo sapiens phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OHu77667	XM_011510785.2	Homo sapiens phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OHu18641	NM_015040.3	Homo sapiens phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$659.50-$923.30 $1319.00
OHu77673	XM_011510792.2	Homo sapiens phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X21, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
OHu77665	XM_011510783.2	Homo sapiens phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PIKFYVE ( NM_152671.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_689884.1 Homo sapiens phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu13271
Clone ID Related Accession (Same CDS sequence)	NM_152671.3
Accession Version	NM_152671.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1356bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Homo sapiens(human)
Product	1-phosphatidylinositol 3-phosphate 5-kinase isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC032389.1. On Jan 10, 2007 this sequence version replaced NM_152671.2. Transcript Variant: This variant (3) lacks two in-frame exons in the 5' coding region and all of the 3' coding region and UTR, compared to variant 2. The encoded protein (isoform 3) has a shorter N-terminus and a short novel C-terminus, compared to isoform 2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC032389.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCACAG ATGATAAGAC GTCCCCAACA CTGGACTCTG CTAATGATTT GCCTCGATCT 
CCTACTAGTC CTTCTCATCT CACACACTTT AAACCTTTGA CTCCTGATCA AGATGAGCCC 
CCTTTTAAAT CAGCTTATAG TTCTTTTGTA AATCTCTTTC GTTTTAACAA AGAGAGAGCA 
GAAGGAGGCC AGGGAGAACA GCAGCCTTTG AGTGGAAGTT GGACCAGCCC TCAGCTCCCT 
TCGAGGACAC AGTCTGTTAG GTCACCCACA CCTTATAAAA AGCAGCTTAA TGAGGAACTC 
CAGCGGCGCT CTTCAGCATT AGGAGACCTC CGAGCTTGCA CATATTGTAG AAAAATAGCC 
TTAAGTTATG CTCATTCCAC AGACAGTAAT TCTATTGGGG AAGACTTGAA TGCTCTTTCA 
GATTCTGCTT GCTCTGTGTC TGTGCTTGAT CCAAGTGAAC CCCGAACACC TGTTGGGAGT 
AGGAAAGCCA GCCGTAACAT ATTTTTAGAG GATGATTTGG CCTGGCAAAG TTTGATTCAT 
CCAGATTCCT CAAATACTCC TCTTTCAACA AGACTTGTAT CTGTGCAAGA GGATGCTGGG 
AAATCTCCTG CTCGAAATAG ATCAGCCAGC ATTACTAACC TGTCACTGGA TAGATCTGGT 
TCTCCTATGG TACCTTCATA TGAGACATCT GTCAGTCCCC AGGCTAACCG AACATATGTT 
AGGACAGAGA CCACTGAGGA TGAACGCAAA ATTCTTCTGG ACAGTGTGCA GTTAAAAGAC 
CTGTGGAAAA AAATCTGCCA TCACAGCAGT GGAATGGAGT TTCAGGATCA CCGCTACTGG 
TTGAGAACGC ATCCCAACTG CATTGTAGGA AAGGAATTAG TCAACTGGCT AATCCGAAAT 
GGGCATATTG CCACAAGGGC ACAAGCTATA GCAATTGGAC AAGCAATGGT TGATGGACGT 
TGGCTGGATT GTGTTAGTCA TCACGACCAG CTTTTCAGAG ATGAGTATGC GCTGTATAGA 
CCACTGCAGA GTACAGAATT TTCTGAGACG CCTTCTCCCG ACAGTGACTC AGTGAACTCC 
GTGGAAGGAC ACTCTGAGCC ATCCTGGTTT AAAGACATAA AGTTTGATGA CAGTGACACA 
GAACAGATAG CTGAAGAAGG TGACGATAAT TTGGCTAATT CTGCCAGTCC TAGCAAGCGC 
ACATCAGTCA GCAGTTTCCA GTCCACAGTG GACAGTGACT CAGCCGCTTC TATCAGCCTG 
AACGTGGAGC TGGACAACGT GAACTTCCAT ATCAAGAAGC CCTCCAAGTA CCCACATGTG 
CCCCCTCACC CTGCTGACCA AAAAGGTAGG AGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_689884.1
CDS	159..1514
Translation	MATDDKTSPTLDSANDLPRSPTSPSHLTHFKPLTPDQDEPPFKSAYSSFVNLFRFNKERAEGGQGEQQPLSGSWTSPQLPSRTQSVRSPTPYKKQLNEELQRRSSALGDLRACTYCRKIALSYAHSTDSNSIGEDLNALSDSACSVSVLDPSEPRTPVGSRKASRNIFLEDDLAWQSLIHPDSSNTPLSTRLVSVQEDAGKSPARNRSASITNLSLDRSGSPMVPSYETSVSPQANRTYVRTETTEDERKILLDSVQLKDLWKKICHHSSGMEFQDHRYWLRTHPNCIVGKELVNWLIRNGHIATRAQAIAIGQAMVDGRWLDCVSHHDQLFRDEYALYRPLQSTEFSETPSPDSDSVNSVEGHSEPSWFKDIKFDDSDTEQIAEEGDDNLANSASPSKRTSVSSFQSTVDSDSAASISLNVELDNVNFHIKKPSKYPHVPPHPADQKGRR

Target ORF information:

RefSeq Version	NM_152671.3
Organism	Homo sapiens(human)
Definition	Homo sapiens phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_152671.3

ORF Insert Sequence:

ATGGCCACAG ATGATAAGAC GTCCCCAACA CTGGACTCTG CTAATGATTT GCCTCGATCT 
CCTACTAGTC CTTCTCATCT CACACACTTT AAACCTTTGA CTCCTGATCA AGATGAGCCC 
CCTTTTAAAT CAGCTTATAG TTCTTTTGTA AATCTCTTTC GTTTTAACAA AGAGAGAGCA 
GAAGGAGGCC AGGGAGAACA GCAGCCTTTG AGTGGAAGTT GGACCAGCCC TCAGCTCCCT 
TCGAGGACAC AGTCTGTTAG GTCACCCACA CCTTATAAAA AGCAGCTTAA TGAGGAACTC 
CAGCGGCGCT CTTCAGCATT AGGAGACCTC CGAGCTTGCA CATATTGTAG AAAAATAGCC 
TTAAGTTATG CTCATTCCAC AGACAGTAAT TCTATTGGGG AAGACTTGAA TGCTCTTTCA 
GATTCTGCTT GCTCTGTGTC TGTGCTTGAT CCAAGTGAAC CCCGAACACC TGTTGGGAGT 
AGGAAAGCCA GCCGTAACAT ATTTTTAGAG GATGATTTGG CCTGGCAAAG TTTGATTCAT 
CCAGATTCCT CAAATACTCC TCTTTCAACA AGACTTGTAT CTGTGCAAGA GGATGCTGGG 
AAATCTCCTG CTCGAAATAG ATCAGCCAGC ATTACTAACC TGTCACTGGA TAGATCTGGT 
TCTCCTATGG TACCTTCATA TGAGACATCT GTCAGTCCCC AGGCTAACCG AACATATGTT 
AGGACAGAGA CCACTGAGGA TGAACGCAAA ATTCTTCTGG ACAGTGTGCA GTTAAAAGAC 
CTGTGGAAAA AAATCTGCCA TCACAGCAGT GGAATGGAGT TTCAGGATCA CCGCTACTGG 
TTGAGAACGC ATCCCAACTG CATTGTAGGA AAGGAATTAG TCAACTGGCT AATCCGAAAT 
GGGCATATTG CCACAAGGGC ACAAGCTATA GCAATTGGAC AAGCAATGGT TGATGGACGT 
TGGCTGGATT GTGTTAGTCA TCACGACCAG CTTTTCAGAG ATGAGTATGC GCTGTATAGA 
CCACTGCAGA GTACAGAATT TTCTGAGACG CCTTCTCCCG ACAGTGACTC AGTGAACTCC 
GTGGAAGGAC ACTCTGAGCC ATCCTGGTTT AAAGACATAA AGTTTGATGA CAGTGACACA 
GAACAGATAG CTGAAGAAGG TGACGATAAT TTGGCTAATT CTGCCAGTCC TAGCAAGCGC 
ACATCAGTCA GCAGTTTCCA GTCCACAGTG GACAGTGACT CAGCCGCTTC TATCAGCCTG 
AACGTGGAGC TGGACAACGT GAACTTCCAT ATCAAGAAGC CCTCCAAGTA CCCACATGTG 
CCCCCTCACC CTGCTGACCA AAAAGGTAGG AGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PIKFYVE ( NM_015040.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_055855.2 Homo sapiens phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu18641
Clone ID Related Accession (Same CDS sequence)	NM_015040.4 , NM_015040.3
Accession Version	NM_015040.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6297bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Homo sapiens(human)
Product	1-phosphatidylinositol 3-phosphate 5-kinase isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC016697.8 and AC012362.7. On Nov 23, 2018 this sequence version replaced NM_015040.3. Transcript Variant: This variant (2) represents the longest transcript and encodes the longest isoform (2). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY457063.1, EU794609.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000264380.9/ ENSP00000264380.4 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCCACAG ATGATAAGAC GTCCCCAACA CTGGACTCTG CTAATGATTT GCCTCGATCT 
CCTACTAGTC CTTCTCATCT CACACACTTT AAACCTTTGA CTCCTGATCA AGATGAGCCC 
CCTTTTAAAT CAGCTTATAG TTCTTTTGTA AATCTCTTTC GTTTTAACAA AGAGAGAGCA 
GAAGGAGGCC AGGGAGAACA GCAGCCTTTG AGTGGAAGTT GGACCAGCCC TCAGCTCCCT 
TCGAGGACAC AGTCTGTTAG GTCACCCACA CCTTATAAAA AGCAGCTTAA TGAGGAACTC 
CAGCGGCGCT CTTCAGCATT AGACACAAGA AGGAAAGCAG AACCTACCTT TGGAGGTCAT 
GACCCTCGTA CAGCTGTTCA GCTTCGAAGC CTCAGCACAG TATTAAAACG CCTCAAGGAA 
ATCATGGAGG GGAAAAGCCA GGATAGTGAC CTGAAACAAT ACTGGATGCC AGATAGCCAA 
TGTAAAGAGT GCTATGACTG TAGTGAGAAA TTTACAACCT TTAGGCGCAG ACACCATTGC 
CGACTATGTG GGCAGATTTT CTGCAGTCGT TGCTGTAATC AAGAAATCCC TGGAAAATTT 
ATGGGCTATA CAGGAGACCT CCGAGCTTGC ACATATTGTA GAAAAATAGC CTTAAGTTAT 
GCTCATTCCA CAGACAGTAA TTCTATTGGG GAAGACTTGA ATGCTCTTTC AGATTCTGCT 
TGCTCTGTGT CTGTGCTTGA TCCAAGTGAA CCCCGAACAC CTGTTGGGAG TAGGAAAGCC 
AGCCGTAACA TATTTTTAGA GGATGATTTG GCCTGGCAAA GTTTGATTCA TCCAGATTCC 
TCAAATACTC CTCTTTCAAC AAGACTTGTA TCTGTGCAAG AGGATGCTGG GAAATCTCCT 
GCTCGAAATA GATCAGCCAG CATTACTAAC CTGTCACTGG ATAGATCTGG TTCTCCTATG 
GTACCTTCAT ATGAGACATC TGTCAGTCCC CAGGCTAACC GAACATATGT TAGGACAGAG 
ACCACTGAGG ATGAACGCAA AATTCTTCTG GACAGTGTGC AGTTAAAAGA CCTGTGGAAA 
AAAATCTGCC ATCACAGCAG TGGAATGGAG TTTCAGGATC ACCGCTACTG GTTGAGAACG 
CATCCCAACT GCATTGTAGG AAAGGAATTA GTCAACTGGC TAATCCGAAA TGGGCATATT 
GCCACAAGGG CACAAGCTAT AGCAATTGGA CAAGCAATGG TTGATGGACG TTGGCTGGAT 
TGTGTTAGTC ATCACGACCA GCTTTTCAGA GATGAGTATG CGCTGTATAG ACCACTGCAG 
AGTACAGAAT TTTCTGAGAC GCCTTCTCCC GACAGTGACT CAGTGAACTC CGTGGAAGGA 
CACTCTGAGC CATCCTGGTT TAAAGACATA AAGTTTGATG ACAGTGACAC AGAACAGATA 
GCTGAAGAAG GTGACGATAA TTTGGCTAAT TCTGCCAGTC CTAGCAAGCG CACATCAGTC 
AGCAGTTTCC AGTCCACAGT GGACAGTGAC TCAGCCGCTT CTATCAGCCT GAACGTGGAG 
CTGGACAACG TGAACTTCCA TATCAAGAAG CCCTCCAAGT ACCCACATGT GCCCCCTCAC 
CCTGCTGACC AAAAAGAGTA TTTGATTTCT GACACTGGAG GACAACAGCT CTCAATAAGT 
GACGCTTTCA TCAAAGAATC CTTATTTAAT CGCCGAGTAG AGGAAAAATC CAAAGAGCTG 
CCTTTCACAC CTTTGGGCTG GCATCATAAC AACCTGGAGC TCCTGAGGGA GGAGAATGGG 
GAGAAACAAG CCATGGAGAG GTTGCTTTCA GCTAATCATA ACCACATGAT GGCACTACTC 
CAGCAGTTGC TCCATAGTGA CTCACTGTCA TCATCTTGGA GGGACATCAT CGTGTCATTG 
GTCTGCCAGG TTGTTCAGAC AGTCCGACCT GATGTCAAGA ACCAGGATGA TGACATGGAT 
ATCCGTCAGT TTGTCCACAT CAAAAAAATC CCAGGTGGAA AGAAGTTTGA TTCTGTGGTT 
GTCAATGGCT TTGTTTGTAC CAAGAACATT GCACATAAAA AGATGAGTTC TTGTATTAAA 
AACCCCAAAA TTCTTCTGTT GAAGTGTTCC ATTGAGTATC TCTACAGAGA AGAAACTAAG 
TTTACTTGCA TTGATCCTAT TGTGCTTCAG GAAAGGGAAT TCTTGAAGAA TTATGTCCAG 
CGAATAGTTG ATGTTCGACC CACCTTGGTT CTTGTTGAGA AAACAGTGTC TCGGATTGCC 
CAGGACATGT TATTGGAACA TGGCATTACT TTGGTCATTA ATGTAAAGTC ACAAGTTTTG 
GAACGAATCA GTCGAATGAC CCAAGGTGAT TTAGTGATGT CAATGGACCA GCTGCTTACG 
AAACCACACC TGGGCACTTG TCACAAATTT TATATGCAGA TATTTCAGTT GCCTAATGAA 
CAAACCAAGA CACTGATGTT TTTTGAAGGT TGTCCACAGC ACCTAGGCTG TACAATCAAG 
CTAAGAGGAG GCTCTGATTA TGAGCTGGCT CGAGTTAAGG AGATCCTAAT ATTTATGATC 
TGTGTTGCTT ATCATTCTCA ACTAGAAATA TCCTTTCTCA TGGATGAATT TGCTATGCCT 
CCCACATTAA TGCAAAACCC TTCATTCCAT TCCCTGATTG AGGGACGAGG GCATGAGGGG 
GCTGTCCAAG AGCAGTACGG TGGAGGTTCC ATCCCCTGGG ATCCTGACAT CCCTCCTGAG 
TCTCTGCCCT GTGATGATAG CAGTTTGCTG GAATTGAGGA TTGTGTTTGA GAAGGGTGAG 
CAGGAAAATA AAAATCTTCC GCAGGCTGTT GCCTCTGTGA AGCATCAAGA ACATAGCACA 
ACAGCTTGCC CGGCGGGTCT CCCTTGTGCT TTCTTTGCAC CTGTACCGGA ATCATTGTTG 
CCACTCCCTG TGGATGACCA ACAAGATGCT TTAGGCAGCG AGCAGCCAGA GACTTTGCAG 
CAAACAGTTG TGCTGCAGGA TCCCAAAAGC CAGATAAGAG CCTTTAGAGA CCCTCTACAG 
GATGACACTG GATTATATGT TACTGAGGAA GTCACCTCCT CTGAAGATAA ACGAAAGACT 
TATTCTTTGG CCTTTAAGCA GGAATTAAAA GATGTGATCC TCTGTATCTC CCCAGTAATC 
ACATTCCGAG AACCCTTTCT TTTAACTGAA AAGGGGATGA GATGCTCTAC CCGAGATTAT 
TTTGCAGAGC AGGTTTACTG GTCTCCTCTC CTCAATAAAG AATTCAAAGA AATGGAGAAC 
AGGAGGAAGA AACAGCTGCT CAGGGATCTC TCTGGACTTC AGGGCATGAA TGGAAGTATT 
CAGGCCAAGT CTATTCAAGT CTTACCCTCA CATGAGCTAG TGAGCACTAG AATTGCTGAG 
CATCTGGGCG ATAGCCAGAG CTTGGGTAGA ATGCTGGCCG ATTATCGAGC CAGAGGAGGA 
AGAATTCAGC CCAAAAATTC AGACCCTTTT GCTCATTCAA AGGATGCATC AAGTACTTCA 
AGTGGCCAAT CAGGAAGCAA AAATGAGGGT GATGAAGAGA GAGGGCTTAT TCTGAGTGAT 
GCTGTGTGGT CAACAAAGGT GGACTGTCTG AATCCCATTA ATCACCAGAG ACTTTGTGTG 
CTCTTCAGCA GCTCTTCTGC CCAGTCCAGC AATGCTCCTA GTGCCTGTGT CAGTCCTTGG 
ATTGTAACAA TGGAATTTTA TGGAAAGAAT GATCTTACAT TAGGAATATT TTTAGAGAGA 
TACTGTTTCA GGCCTTCTTA TCAGTGTCCA AGCATGTTCT GTGATACCCC CATGGTACAT 
CATATTCGGC GCTTTGTTCA TGGCCAAGGC TGTGTGCAGA TAATCCTGAA GGAGTTGGAT 
TCTCCAGTAC CTGGATATCA GCATACAATT CTTACATATT CCTGGTGTAG AATCTGCAAA 
CAGGTAACAC CAGTTGTTGC TCTTTCCAAT GAGTCCTGGT CTATGTCATT TGCAAAATAC 
CTTGAACTTA GGTTTTATGG GCACCAGTAT ACTCGCAGAG CCAACGCTGA GCCCTGTGGT 
CACTCCATCC ATCATGATTA TCACCAGTAT TTCTCCTATA ACCAGATGGT GGCGTCTTTC 
AGTTATTCTC CCATTCGGCT TCTTGAAGTA TGTGTTCCAC TCCCCAAAAT ATTCATTAAG 
CGTCAGGCCC CATTAAAAGT GTCCCTTCTT CAGGATCTGA AGGACTTCTT TCAAAAAGTT 
TCACAGGTAT ATGTTGCCAT TGATGAAAGA CTTGCATCTT TGAAAACTGA TACATTTAGT 
AAAACAAGAG AGGAAAAAAT GGAAGATATT TTTGCACAGA AAGAGATGGA AGAAGGTGAG 
TTCAAGAACT GGATTGAGAA GATGCAAGCA AGGCTCATGT CTTCCTCTGT AGATACCCCT 
CAGCAACTGC AGTCGGTCTT TGAGTCACTC ATTGCCAAGA AACAAAGTCT CTGTGAAGTG 
CTGCAAGCTT GGAATAACAG GTTGCAGGAC CTTTTCCAAC AGGAAAAGGG TAGAAAGAGA 
CCTTCAGTTC CTCCAAGTCC TGGAAGACTG AGACAAGGGG AAGAAAGCAA GATAAGTGCG 
ATGGATGCAT CTCCACGGAA TATTTCTCCA GGACTTCAGA ATGGAGAAAA AGAGGATCGC 
TTCTTAACAA CTTTGTCCAG CCAGAGCTCC ACCAGTTCTA CTCATCTCCA ATTGCCTACG 
CCACCTGAAG TCATGTCTGA ACAGTCAGTG GGAGGGCCCC CTGAGCTAGA TACAGCCAGC 
AGTTCCGAAG ATGTGTTTGA TGGGCATTTG CTGGGATCCA CAGACAGCCA AGTGAAGGAA 
AAGTCAACCA TGAAAGCCAT CTTTGCAAAT TTGCTTCCAG GAAATAGCTA TAATCCTATT 
CCATTTCCTT TTGATCCAGA TAAACACTAC TTAATGTATG AACATGAACG AGTGCCCATT 
GCAGTCTGCG AGAAGGAACC CAGCTCCATC ATTGCTTTTG CTCTCAGTTG TAAAGAATAC 
CGAAATGCCT TAGAGGAATT GTCTAAAGCG ACTCAGTGGA ACAGTGCCGA AGAAGGGCTT 
CCAACAAATA GTACTTCAGA TAGCAGACCA AAGAGTAGCA GCCCTATCAG ATTACCTGAA 
ATGAGTGGAG GACAGACAAA TCGTACAACA GAAACAGAAC CACAACCAAC CAAAAAGGCT 
TCTGGAATGT TGTCCTTCTT CAGAGGGACA GCAGGGAAAA GCCCCGATCT CTCTTCCCAG 
AAGAGAGAGA CCTTACGTGG AGCAGATAGT GCTTACTACC AGGTTGGGCA GACGGGCAAG 
GAGGGGACCG AGAATCAAGG CGTTGAGCCT CAAGATGAAG TAGATGGAGG AGATACACAA 
AAGAAGCAAC TCATAAATCC TCATGTGGAA CTTCAATTTT CAGATGCTAA TGCCAAGTTT 
TACTGTCGGC TCTACTATGC GGGAGAGTTT CATAAGATGC GTGAAGTGAT TCTGGACAGC 
AGTGAAGAAG ATTTCATTCG TTCCCTCTCC CACTCATCAC CCTGGCAGGC CCGGGGAGGC 
AAATCAGGAG CTGCCTTCTA TGCAACTGAG GATGATAGAT TTATTTTGAA GCAAATGCCT 
CGTCTGGAAG TCCAGTCCTT CCTCGACTTT GCACCACATT ACTTCAATTA TATTACAAAT 
GCTGTTCAAC AAAAGAGGCC CACGGCGTTG GCCAAAATTC TTGGAGTTTA CAGAATTGGT 
TATAAGAACT CTCAGAACAA CACTGAGAAG AAGTTAGATC TCCTTGTCAT GGAAAATCTT 
TTCTACGGGA GAAAGATGGC ACAGGTTTTT GATTTGAAGG GCTCTCTTAG GAATCGGAAT 
GTAAAAACTG ACACTGGAAA AGAGAGTTGT GATGTGGTCC TGCTAGATGA AAATCTCCTA 
AAGATGGTTC GAGACAACCC TCTATATATT CGTTCTCATT CCAAAGCTGT GCTGAGAACC 
TCGATCCATA GTGACTCCCA TTTCCTTTCT AGCCACCTCA TTATAGATTA TTCTTTGCTG 
GTTGGGCGAG ATGATACTAG CAATGAGCTA GTAGTTGGAA TTATAGATTA TATTCGAACA 
TTTACATGGG ACAAAAAGCT TGAGATGGTT GTGAAATCAA CAGGAATTTT AGGTGGACAA 
GGTAAAATGC CAACAGTGGT GTCTCCGGAG TTGTACAGGA CTAGGTTTTG TGAGGCAATG 
GACAAGTATT TCCTAATGGT ACCAGACCAC TGGACAGGCT TGGGTCTGAA TTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_055855.2
CDS	171..6467
Translation	MATDDKTSPTLDSANDLPRSPTSPSHLTHFKPLTPDQDEPPFKSAYSSFVNLFRFNKERAEGGQGEQQPLSGSWTSPQLPSRTQSVRSPTPYKKQLNEELQRRSSALDTRRKAEPTFGGHDPRTAVQLRSLSTVLKRLKEIMEGKSQDSDLKQYWMPDSQCKECYDCSEKFTTFRRRHHCRLCGQIFCSRCCNQEIPGKFMGYTGDLRACTYCRKIALSYAHSTDSNSIGEDLNALSDSACSVSVLDPSEPRTPVGSRKASRNIFLEDDLAWQSLIHPDSSNTPLSTRLVSVQEDAGKSPARNRSASITNLSLDRSGSPMVPSYETSVSPQANRTYVRTETTEDERKILLDSVQLKDLWKKICHHSSGMEFQDHRYWLRTHPNCIVGKELVNWLIRNGHIATRAQAIAIGQAMVDGRWLDCVSHHDQLFRDEYALYRPLQSTEFSETPSPDSDSVNSVEGHSEPSWFKDIKFDDSDTEQIAEEGDDNLANSASPSKRTSVSSFQSTVDSDSAASISLNVELDNVNFHIKKPSKYPHVPPHPADQKEYLISDTGGQQLSISDAFIKESLFNRRVEEKSKELPFTPLGWHHNNLELLREENGEKQAMERLLSANHNHMMALLQQLLHSDSLSSSWRDIIVSLVCQVVQTVRPDVKNQDDDMDIRQFVHIKKIPGGKKFDSVVVNGFVCTKNIAHKKMSSCIKNPKILLLKCSIEYLYREETKFTCIDPIVLQEREFLKNYVQRIVDVRPTLVLVEKTVSRIAQDMLLEHGITLVINVKSQVLERISRMTQGDLVMSMDQLLTKPHLGTCHKFYMQIFQLPNEQTKTLMFFEGCPQHLGCTIKLRGGSDYELARVKEILIFMICVAYHSQLEISFLMDEFAMPPTLMQNPSFHSLIEGRGHEGAVQEQYGGGSIPWDPDIPPESLPCDDSSLLELRIVFEKGEQENKNLPQAVASVKHQEHSTTACPAGLPCAFFAPVPESLLPLPVDDQQDALGSEQPETLQQTVVLQDPKSQIRAFRDPLQDDTGLYVTEEVTSSEDKRKTYSLAFKQELKDVILCISPVITFREPFLLTEKGMRCSTRDYFAEQVYWSPLLNKEFKEMENRRKKQLLRDLSGLQGMNGSIQAKSIQVLPSHELVSTRIAEHLGDSQSLGRMLADYRARGGRIQPKNSDPFAHSKDASSTSSGQSGSKNEGDEERGLILSDAVWSTKVDCLNPINHQRLCVLFSSSSAQSSNAPSACVSPWIVTMEFYGKNDLTLGIFLERYCFRPSYQCPSMFCDTPMVHHIRRFVHGQGCVQIILKELDSPVPGYQHTILTYSWCRICKQVTPVVALSNESWSMSFAKYLELRFYGHQYTRRANAEPCGHSIHHDYHQYFSYNQMVASFSYSPIRLLEVCVPLPKIFIKRQAPLKVSLLQDLKDFFQKVSQVYVAIDERLASLKTDTFSKTREEKMEDIFAQKEMEEGEFKNWIEKMQARLMSSSVDTPQQLQSVFESLIAKKQSLCEVLQAWNNRLQDLFQQEKGRKRPSVPPSPGRLRQGEESKISAMDASPRNISPGLQNGEKEDRFLTTLSSQSSTSSTHLQLPTPPEVMSEQSVGGPPELDTASSSEDVFDGHLLGSTDSQVKEKSTMKAIFANLLPGNSYNPIPFPFDPDKHYLMYEHERVPIAVCEKEPSSIIAFALSCKEYRNALEELSKATQWNSAEEGLPTNSTSDSRPKSSSPIRLPEMSGGQTNRTTETEPQPTKKASGMLSFFRGTAGKSPDLSSQKRETLRGADSAYYQVGQTGKEGTENQGVEPQDEVDGGDTQKKQLINPHVELQFSDANAKFYCRLYYAGEFHKMREVILDSSEEDFIRSLSHSSPWQARGGKSGAAFYATEDDRFILKQMPRLEVQSFLDFAPHYFNYITNAVQQKRPTALAKILGVYRIGYKNSQNNTEKKLDLLVMENLFYGRKMAQVFDLKGSLRNRNVKTDTGKESCDVVLLDENLLKMVRDNPLYIRSHSKAVLRTSIHSDSHFLSSHLIIDYSLLVGRDDTSNELVVGIIDYIRTFTWDKKLEMVVKSTGILGGQGKMPTVVSPELYRTRFCEAMDKYFLMVPDHWTGLGLNC

Target ORF information:

RefSeq Version	NM_015040.4
Organism	Homo sapiens(human)
Definition	Homo sapiens phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_015040.4

ORF Insert Sequence:

ATGGCCACAG ATGATAAGAC GTCCCCAACA CTGGACTCTG CTAATGATTT GCCTCGATCT 
CCTACTAGTC CTTCTCATCT CACACACTTT AAACCTTTGA CTCCTGATCA AGATGAGCCC 
CCTTTTAAAT CAGCTTATAG TTCTTTTGTA AATCTCTTTC GTTTTAACAA AGAGAGAGCA 
GAAGGAGGCC AGGGAGAACA GCAGCCTTTG AGTGGAAGTT GGACCAGCCC TCAGCTCCCT 
TCGAGGACAC AGTCTGTTAG GTCACCCACA CCTTATAAAA AGCAGCTTAA TGAGGAACTC 
CAGCGGCGCT CTTCAGCATT AGACACAAGA AGGAAAGCAG AACCTACCTT TGGAGGTCAT 
GACCCTCGTA CAGCTGTTCA GCTTCGAAGC CTCAGCACAG TATTAAAACG CCTCAAGGAA 
ATCATGGAGG GGAAAAGCCA GGATAGTGAC CTGAAACAAT ACTGGATGCC AGATAGCCAA 
TGTAAAGAGT GCTATGACTG TAGTGAGAAA TTTACAACCT TTAGGCGCAG ACACCATTGC 
CGACTATGTG GGCAGATTTT CTGCAGTCGT TGCTGTAATC AAGAAATCCC TGGAAAATTT 
ATGGGCTATA CAGGAGACCT CCGAGCTTGC ACATATTGTA GAAAAATAGC CTTAAGTTAT 
GCTCATTCCA CAGACAGTAA TTCTATTGGG GAAGACTTGA ATGCTCTTTC AGATTCTGCT 
TGCTCTGTGT CTGTGCTTGA TCCAAGTGAA CCCCGAACAC CTGTTGGGAG TAGGAAAGCC 
AGCCGTAACA TATTTTTAGA GGATGATTTG GCCTGGCAAA GTTTGATTCA TCCAGATTCC 
TCAAATACTC CTCTTTCAAC AAGACTTGTA TCTGTGCAAG AGGATGCTGG GAAATCTCCT 
GCTCGAAATA GATCAGCCAG CATTACTAAC CTGTCACTGG ATAGATCTGG TTCTCCTATG 
GTACCTTCAT ATGAGACATC TGTCAGTCCC CAGGCTAACC GAACATATGT TAGGACAGAG 
ACCACTGAGG ATGAACGCAA AATTCTTCTG GACAGTGTGC AGTTAAAAGA CCTGTGGAAA 
AAAATCTGCC ATCACAGCAG TGGAATGGAG TTTCAGGATC ACCGCTACTG GTTGAGAACG 
CATCCCAACT GCATTGTAGG AAAGGAATTA GTCAACTGGC TAATCCGAAA TGGGCATATT 
GCCACAAGGG CACAAGCTAT AGCAATTGGA CAAGCAATGG TTGATGGACG TTGGCTGGAT 
TGTGTTAGTC ATCACGACCA GCTTTTCAGA GATGAGTATG CGCTGTATAG ACCACTGCAG 
AGTACAGAAT TTTCTGAGAC GCCTTCTCCC GACAGTGACT CAGTGAACTC CGTGGAAGGA 
CACTCTGAGC CATCCTGGTT TAAAGACATA AAGTTTGATG ACAGTGACAC AGAACAGATA 
GCTGAAGAAG GTGACGATAA TTTGGCTAAT TCTGCCAGTC CTAGCAAGCG CACATCAGTC 
AGCAGTTTCC AGTCCACAGT GGACAGTGAC TCAGCCGCTT CTATCAGCCT GAACGTGGAG 
CTGGACAACG TGAACTTCCA TATCAAGAAG CCCTCCAAGT ACCCACATGT GCCCCCTCAC 
CCTGCTGACC AAAAAGAGTA TTTGATTTCT GACACTGGAG GACAACAGCT CTCAATAAGT 
GACGCTTTCA TCAAAGAATC CTTATTTAAT CGCCGAGTAG AGGAAAAATC CAAAGAGCTG 
CCTTTCACAC CTTTGGGCTG GCATCATAAC AACCTGGAGC TCCTGAGGGA GGAGAATGGG 
GAGAAACAAG CCATGGAGAG GTTGCTTTCA GCTAATCATA ACCACATGAT GGCACTACTC 
CAGCAGTTGC TCCATAGTGA CTCACTGTCA TCATCTTGGA GGGACATCAT CGTGTCATTG 
GTCTGCCAGG TTGTTCAGAC AGTCCGACCT GATGTCAAGA ACCAGGATGA TGACATGGAT 
ATCCGTCAGT TTGTCCACAT CAAAAAAATC CCAGGTGGAA AGAAGTTTGA TTCTGTGGTT 
GTCAATGGCT TTGTTTGTAC CAAGAACATT GCACATAAAA AGATGAGTTC TTGTATTAAA 
AACCCCAAAA TTCTTCTGTT GAAGTGTTCC ATTGAGTATC TCTACAGAGA AGAAACTAAG 
TTTACTTGCA TTGATCCTAT TGTGCTTCAG GAAAGGGAAT TCTTGAAGAA TTATGTCCAG 
CGAATAGTTG ATGTTCGACC CACCTTGGTT CTTGTTGAGA AAACAGTGTC TCGGATTGCC 
CAGGACATGT TATTGGAACA TGGCATTACT TTGGTCATTA ATGTAAAGTC ACAAGTTTTG 
GAACGAATCA GTCGAATGAC CCAAGGTGAT TTAGTGATGT CAATGGACCA GCTGCTTACG 
AAACCACACC TGGGCACTTG TCACAAATTT TATATGCAGA TATTTCAGTT GCCTAATGAA 
CAAACCAAGA CACTGATGTT TTTTGAAGGT TGTCCACAGC ACCTAGGCTG TACAATCAAG 
CTAAGAGGAG GCTCTGATTA TGAGCTGGCT CGAGTTAAGG AGATCCTAAT ATTTATGATC 
TGTGTTGCTT ATCATTCTCA ACTAGAAATA TCCTTTCTCA TGGATGAATT TGCTATGCCT 
CCCACATTAA TGCAAAACCC TTCATTCCAT TCCCTGATTG AGGGACGAGG GCATGAGGGG 
GCTGTCCAAG AGCAGTACGG TGGAGGTTCC ATCCCCTGGG ATCCTGACAT CCCTCCTGAG 
TCTCTGCCCT GTGATGATAG CAGTTTGCTG GAATTGAGGA TTGTGTTTGA GAAGGGTGAG 
CAGGAAAATA AAAATCTTCC GCAGGCTGTT GCCTCTGTGA AGCATCAAGA ACATAGCACA 
ACAGCTTGCC CGGCGGGTCT CCCTTGTGCT TTCTTTGCAC CTGTACCGGA ATCATTGTTG 
CCACTCCCTG TGGATGACCA ACAAGATGCT TTAGGCAGCG AGCAGCCAGA GACTTTGCAG 
CAAACAGTTG TGCTGCAGGA TCCCAAAAGC CAGATAAGAG CCTTTAGAGA CCCTCTACAG 
GATGACACTG GATTATATGT TACTGAGGAA GTCACCTCCT CTGAAGATAA ACGAAAGACT 
TATTCTTTGG CCTTTAAGCA GGAATTAAAA GATGTGATCC TCTGTATCTC CCCAGTAATC 
ACATTCCGAG AACCCTTTCT TTTAACTGAA AAGGGGATGA GATGCTCTAC CCGAGATTAT 
TTTGCAGAGC AGGTTTACTG GTCTCCTCTC CTCAATAAAG AATTCAAAGA AATGGAGAAC 
AGGAGGAAGA AACAGCTGCT CAGGGATCTC TCTGGACTTC AGGGCATGAA TGGAAGTATT 
CAGGCCAAGT CTATTCAAGT CTTACCCTCA CATGAGCTAG TGAGCACTAG AATTGCTGAG 
CATCTGGGCG ATAGCCAGAG CTTGGGTAGA ATGCTGGCCG ATTATCGAGC CAGAGGAGGA 
AGAATTCAGC CCAAAAATTC AGACCCTTTT GCTCATTCAA AGGATGCATC AAGTACTTCA 
AGTGGCCAAT CAGGAAGCAA AAATGAGGGT GATGAAGAGA GAGGGCTTAT TCTGAGTGAT 
GCTGTGTGGT CAACAAAGGT GGACTGTCTG AATCCCATTA ATCACCAGAG ACTTTGTGTG 
CTCTTCAGCA GCTCTTCTGC CCAGTCCAGC AATGCTCCTA GTGCCTGTGT CAGTCCTTGG 
ATTGTAACAA TGGAATTTTA TGGAAAGAAT GATCTTACAT TAGGAATATT TTTAGAGAGA 
TACTGTTTCA GGCCTTCTTA TCAGTGTCCA AGCATGTTCT GTGATACCCC CATGGTACAT 
CATATTCGGC GCTTTGTTCA TGGCCAAGGC TGTGTGCAGA TAATCCTGAA GGAGTTGGAT 
TCTCCAGTAC CTGGATATCA GCATACAATT CTTACATATT CCTGGTGTAG AATCTGCAAA 
CAGGTAACAC CAGTTGTTGC TCTTTCCAAT GAGTCCTGGT CTATGTCATT TGCAAAATAC 
CTTGAACTTA GGTTTTATGG GCACCAGTAT ACTCGCAGAG CCAACGCTGA GCCCTGTGGT 
CACTCCATCC ATCATGATTA TCACCAGTAT TTCTCCTATA ACCAGATGGT GGCGTCTTTC 
AGTTATTCTC CCATTCGGCT TCTTGAAGTA TGTGTTCCAC TCCCCAAAAT ATTCATTAAG 
CGTCAGGCCC CATTAAAAGT GTCCCTTCTT CAGGATCTGA AGGACTTCTT TCAAAAAGTT 
TCACAGGTAT ATGTTGCCAT TGATGAAAGA CTTGCATCTT TGAAAACTGA TACATTTAGT 
AAAACAAGAG AGGAAAAAAT GGAAGATATT TTTGCACAGA AAGAGATGGA AGAAGGTGAG 
TTCAAGAACT GGATTGAGAA GATGCAAGCA AGGCTCATGT CTTCCTCTGT AGATACCCCT 
CAGCAACTGC AGTCGGTCTT TGAGTCACTC ATTGCCAAGA AACAAAGTCT CTGTGAAGTG 
CTGCAAGCTT GGAATAACAG GTTGCAGGAC CTTTTCCAAC AGGAAAAGGG TAGAAAGAGA 
CCTTCAGTTC CTCCAAGTCC TGGAAGACTG AGACAAGGGG AAGAAAGCAA GATAAGTGCG 
ATGGATGCAT CTCCACGGAA TATTTCTCCA GGACTTCAGA ATGGAGAAAA AGAGGATCGC 
TTCTTAACAA CTTTGTCCAG CCAGAGCTCC ACCAGTTCTA CTCATCTCCA ATTGCCTACG 
CCACCTGAAG TCATGTCTGA ACAGTCAGTG GGAGGGCCCC CTGAGCTAGA TACAGCCAGC 
AGTTCCGAAG ATGTGTTTGA TGGGCATTTG CTGGGATCCA CAGACAGCCA AGTGAAGGAA 
AAGTCAACCA TGAAAGCCAT CTTTGCAAAT TTGCTTCCAG GAAATAGCTA TAATCCTATT 
CCATTTCCTT TTGATCCAGA TAAACACTAC TTAATGTATG AACATGAACG AGTGCCCATT 
GCAGTCTGCG AGAAGGAACC CAGCTCCATC ATTGCTTTTG CTCTCAGTTG TAAAGAATAC 
CGAAATGCCT TAGAGGAATT GTCTAAAGCG ACTCAGTGGA ACAGTGCCGA AGAAGGGCTT 
CCAACAAATA GTACTTCAGA TAGCAGACCA AAGAGTAGCA GCCCTATCAG ATTACCTGAA 
ATGAGTGGAG GACAGACAAA TCGTACAACA GAAACAGAAC CACAACCAAC CAAAAAGGCT 
TCTGGAATGT TGTCCTTCTT CAGAGGGACA GCAGGGAAAA GCCCCGATCT CTCTTCCCAG 
AAGAGAGAGA CCTTACGTGG AGCAGATAGT GCTTACTACC AGGTTGGGCA GACGGGCAAG 
GAGGGGACCG AGAATCAAGG CGTTGAGCCT CAAGATGAAG TAGATGGAGG AGATACACAA 
AAGAAGCAAC TCATAAATCC TCATGTGGAA CTTCAATTTT CAGATGCTAA TGCCAAGTTT 
TACTGTCGGC TCTACTATGC GGGAGAGTTT CATAAGATGC GTGAAGTGAT TCTGGACAGC 
AGTGAAGAAG ATTTCATTCG TTCCCTCTCC CACTCATCAC CCTGGCAGGC CCGGGGAGGC 
AAATCAGGAG CTGCCTTCTA TGCAACTGAG GATGATAGAT TTATTTTGAA GCAAATGCCT 
CGTCTGGAAG TCCAGTCCTT CCTCGACTTT GCACCACATT ACTTCAATTA TATTACAAAT 
GCTGTTCAAC AAAAGAGGCC CACGGCGTTG GCCAAAATTC TTGGAGTTTA CAGAATTGGT 
TATAAGAACT CTCAGAACAA CACTGAGAAG AAGTTAGATC TCCTTGTCAT GGAAAATCTT 
TTCTACGGGA GAAAGATGGC ACAGGTTTTT GATTTGAAGG GCTCTCTTAG GAATCGGAAT 
GTAAAAACTG ACACTGGAAA AGAGAGTTGT GATGTGGTCC TGCTAGATGA AAATCTCCTA 
AAGATGGTTC GAGACAACCC TCTATATATT CGTTCTCATT CCAAAGCTGT GCTGAGAACC 
TCGATCCATA GTGACTCCCA TTTCCTTTCT AGCCACCTCA TTATAGATTA TTCTTTGCTG 
GTTGGGCGAG ATGATACTAG CAATGAGCTA GTAGTTGGAA TTATAGATTA TATTCGAACA 
TTTACATGGG ACAAAAAGCT TGAGATGGTT GTGAAATCAA CAGGAATTTT AGGTGGACAA 
GGTAAAATGC CAACAGTGGT GTCTCCGGAG TTGTACAGGA CTAGGTTTTG TGAGGCAATG 
GACAAGTATT TCCTAATGGT ACCAGACCAC TGGACAGGCT TGGGTCTGAA TTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PIKFYVE ( NM_015040.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_055855.2 Homo sapiens phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant 2, mRNA.

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CloneID	OHu18641
Clone ID Related Accession (Same CDS sequence)	NM_015040.4 , NM_015040.3
Accession Version	NM_015040.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6297bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-15
Organism	Homo sapiens(human)
Product	1-phosphatidylinositol 3-phosphate 5-kinase isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AY457063.1, AC012362.7 and AB023198.1. This sequence is a reference standard in the RefSeqGene project. On Jan 10, 2007 this sequence version replaced NM_015040.2. Transcript Variant: This variant (2) represents the longest transcript and encodes the longest isoform (2). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY457063.1, EU794609.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCACAG ATGATAAGAC GTCCCCAACA CTGGACTCTG CTAATGATTT GCCTCGATCT 
CCTACTAGTC CTTCTCATCT CACACACTTT AAACCTTTGA CTCCTGATCA AGATGAGCCC 
CCTTTTAAAT CAGCTTATAG TTCTTTTGTA AATCTCTTTC GTTTTAACAA AGAGAGAGCA 
GAAGGAGGCC AGGGAGAACA GCAGCCTTTG AGTGGAAGTT GGACCAGCCC TCAGCTCCCT 
TCGAGGACAC AGTCTGTTAG GTCACCCACA CCTTATAAAA AGCAGCTTAA TGAGGAACTC 
CAGCGGCGCT CTTCAGCATT AGACACAAGA AGGAAAGCAG AACCTACCTT TGGAGGTCAT 
GACCCTCGTA CAGCTGTTCA GCTTCGAAGC CTCAGCACAG TATTAAAACG CCTCAAGGAA 
ATCATGGAGG GGAAAAGCCA GGATAGTGAC CTGAAACAAT ACTGGATGCC AGATAGCCAA 
TGTAAAGAGT GCTATGACTG TAGTGAGAAA TTTACAACCT TTAGGCGCAG ACACCATTGC 
CGACTATGTG GGCAGATTTT CTGCAGTCGT TGCTGTAATC AAGAAATCCC TGGAAAATTT 
ATGGGCTATA CAGGAGACCT CCGAGCTTGC ACATATTGTA GAAAAATAGC CTTAAGTTAT 
GCTCATTCCA CAGACAGTAA TTCTATTGGG GAAGACTTGA ATGCTCTTTC AGATTCTGCT 
TGCTCTGTGT CTGTGCTTGA TCCAAGTGAA CCCCGAACAC CTGTTGGGAG TAGGAAAGCC 
AGCCGTAACA TATTTTTAGA GGATGATTTG GCCTGGCAAA GTTTGATTCA TCCAGATTCC 
TCAAATACTC CTCTTTCAAC AAGACTTGTA TCTGTGCAAG AGGATGCTGG GAAATCTCCT 
GCTCGAAATA GATCAGCCAG CATTACTAAC CTGTCACTGG ATAGATCTGG TTCTCCTATG 
GTACCTTCAT ATGAGACATC TGTCAGTCCC CAGGCTAACC GAACATATGT TAGGACAGAG 
ACCACTGAGG ATGAACGCAA AATTCTTCTG GACAGTGTGC AGTTAAAAGA CCTGTGGAAA 
AAAATCTGCC ATCACAGCAG TGGAATGGAG TTTCAGGATC ACCGCTACTG GTTGAGAACG 
CATCCCAACT GCATTGTAGG AAAGGAATTA GTCAACTGGC TAATCCGAAA TGGGCATATT 
GCCACAAGGG CACAAGCTAT AGCAATTGGA CAAGCAATGG TTGATGGACG TTGGCTGGAT 
TGTGTTAGTC ATCACGACCA GCTTTTCAGA GATGAGTATG CGCTGTATAG ACCACTGCAG 
AGTACAGAAT TTTCTGAGAC GCCTTCTCCC GACAGTGACT CAGTGAACTC CGTGGAAGGA 
CACTCTGAGC CATCCTGGTT TAAAGACATA AAGTTTGATG ACAGTGACAC AGAACAGATA 
GCTGAAGAAG GTGACGATAA TTTGGCTAAT TCTGCCAGTC CTAGCAAGCG CACATCAGTC 
AGCAGTTTCC AGTCCACAGT GGACAGTGAC TCAGCCGCTT CTATCAGCCT GAACGTGGAG 
CTGGACAACG TGAACTTCCA TATCAAGAAG CCCTCCAAGT ACCCACATGT GCCCCCTCAC 
CCTGCTGACC AAAAAGAGTA TTTGATTTCT GACACTGGAG GACAACAGCT CTCAATAAGT 
GACGCTTTCA TCAAAGAATC CTTATTTAAT CGCCGAGTAG AGGAAAAATC CAAAGAGCTG 
CCTTTCACAC CTTTGGGCTG GCATCATAAC AACCTGGAGC TCCTGAGGGA GGAGAATGGG 
GAGAAACAAG CCATGGAGAG GTTGCTTTCA GCTAATCATA ACCACATGAT GGCACTACTC 
CAGCAGTTGC TCCATAGTGA CTCACTGTCA TCATCTTGGA GGGACATCAT CGTGTCATTG 
GTCTGCCAGG TTGTTCAGAC AGTCCGACCT GATGTCAAGA ACCAGGATGA TGACATGGAT 
ATCCGTCAGT TTGTCCACAT CAAAAAAATC CCAGGTGGAA AGAAGTTTGA TTCTGTGGTT 
GTCAATGGCT TTGTTTGTAC CAAGAACATT GCACATAAAA AGATGAGTTC TTGTATTAAA 
AACCCCAAAA TTCTTCTGTT GAAGTGTTCC ATTGAGTATC TCTACAGAGA AGAAACTAAG 
TTTACTTGCA TTGATCCTAT TGTGCTTCAG GAAAGGGAAT TCTTGAAGAA TTATGTCCAG 
CGAATAGTTG ATGTTCGACC CACCTTGGTT CTTGTTGAGA AAACAGTGTC TCGGATTGCC 
CAGGACATGT TATTGGAACA TGGCATTACT TTGGTCATTA ATGTAAAGTC ACAAGTTTTG 
GAACGAATCA GTCGAATGAC CCAAGGTGAT TTAGTGATGT CAATGGACCA GCTGCTTACG 
AAACCACACC TGGGCACTTG TCACAAATTT TATATGCAGA TATTTCAGTT GCCTAATGAA 
CAAACCAAGA CACTGATGTT TTTTGAAGGT TGTCCACAGC ACCTAGGCTG TACAATCAAG 
CTAAGAGGAG GCTCTGATTA TGAGCTGGCT CGAGTTAAGG AGATCCTAAT ATTTATGATC 
TGTGTTGCTT ATCATTCTCA ACTAGAAATA TCCTTTCTCA TGGATGAATT TGCTATGCCT 
CCCACATTAA TGCAAAACCC TTCATTCCAT TCCCTGATTG AGGGACGAGG GCATGAGGGG 
GCTGTCCAAG AGCAGTACGG TGGAGGTTCC ATCCCCTGGG ATCCTGACAT CCCTCCTGAG 
TCTCTGCCCT GTGATGATAG CAGTTTGCTG GAATTGAGGA TTGTGTTTGA GAAGGGTGAG 
CAGGAAAATA AAAATCTTCC GCAGGCTGTT GCCTCTGTGA AGCATCAAGA ACATAGCACA 
ACAGCTTGCC CGGCGGGTCT CCCTTGTGCT TTCTTTGCAC CTGTACCGGA ATCATTGTTG 
CCACTCCCTG TGGATGACCA ACAAGATGCT TTAGGCAGCG AGCAGCCAGA GACTTTGCAG 
CAAACAGTTG TGCTGCAGGA TCCCAAAAGC CAGATAAGAG CCTTTAGAGA CCCTCTACAG 
GATGACACTG GATTATATGT TACTGAGGAA GTCACCTCCT CTGAAGATAA ACGAAAGACT 
TATTCTTTGG CCTTTAAGCA GGAATTAAAA GATGTGATCC TCTGTATCTC CCCAGTAATC 
ACATTCCGAG AACCCTTTCT TTTAACTGAA AAGGGGATGA GATGCTCTAC CCGAGATTAT 
TTTGCAGAGC AGGTTTACTG GTCTCCTCTC CTCAATAAAG AATTCAAAGA AATGGAGAAC 
AGGAGGAAGA AACAGCTGCT CAGGGATCTC TCTGGACTTC AGGGCATGAA TGGAAGTATT 
CAGGCCAAGT CTATTCAAGT CTTACCCTCA CATGAGCTAG TGAGCACTAG AATTGCTGAG 
CATCTGGGCG ATAGCCAGAG CTTGGGTAGA ATGCTGGCCG ATTATCGAGC CAGAGGAGGA 
AGAATTCAGC CCAAAAATTC AGACCCTTTT GCTCATTCAA AGGATGCATC AAGTACTTCA 
AGTGGCCAAT CAGGAAGCAA AAATGAGGGT GATGAAGAGA GAGGGCTTAT TCTGAGTGAT 
GCTGTGTGGT CAACAAAGGT GGACTGTCTG AATCCCATTA ATCACCAGAG ACTTTGTGTG 
CTCTTCAGCA GCTCTTCTGC CCAGTCCAGC AATGCTCCTA GTGCCTGTGT CAGTCCTTGG 
ATTGTAACAA TGGAATTTTA TGGAAAGAAT GATCTTACAT TAGGAATATT TTTAGAGAGA 
TACTGTTTCA GGCCTTCTTA TCAGTGTCCA AGCATGTTCT GTGATACCCC CATGGTACAT 
CATATTCGGC GCTTTGTTCA TGGCCAAGGC TGTGTGCAGA TAATCCTGAA GGAGTTGGAT 
TCTCCAGTAC CTGGATATCA GCATACAATT CTTACATATT CCTGGTGTAG AATCTGCAAA 
CAGGTAACAC CAGTTGTTGC TCTTTCCAAT GAGTCCTGGT CTATGTCATT TGCAAAATAC 
CTTGAACTTA GGTTTTATGG GCACCAGTAT ACTCGCAGAG CCAACGCTGA GCCCTGTGGT 
CACTCCATCC ATCATGATTA TCACCAGTAT TTCTCCTATA ACCAGATGGT GGCGTCTTTC 
AGTTATTCTC CCATTCGGCT TCTTGAAGTA TGTGTTCCAC TCCCCAAAAT ATTCATTAAG 
CGTCAGGCCC CATTAAAAGT GTCCCTTCTT CAGGATCTGA AGGACTTCTT TCAAAAAGTT 
TCACAGGTAT ATGTTGCCAT TGATGAAAGA CTTGCATCTT TGAAAACTGA TACATTTAGT 
AAAACAAGAG AGGAAAAAAT GGAAGATATT TTTGCACAGA AAGAGATGGA AGAAGGTGAG 
TTCAAGAACT GGATTGAGAA GATGCAAGCA AGGCTCATGT CTTCCTCTGT AGATACCCCT 
CAGCAACTGC AGTCGGTCTT TGAGTCACTC ATTGCCAAGA AACAAAGTCT CTGTGAAGTG 
CTGCAAGCTT GGAATAACAG GTTGCAGGAC CTTTTCCAAC AGGAAAAGGG TAGAAAGAGA 
CCTTCAGTTC CTCCAAGTCC TGGAAGACTG AGACAAGGGG AAGAAAGCAA GATAAGTGCG 
ATGGATGCAT CTCCACGGAA TATTTCTCCA GGACTTCAGA ATGGAGAAAA AGAGGATCGC 
TTCTTAACAA CTTTGTCCAG CCAGAGCTCC ACCAGTTCTA CTCATCTCCA ATTGCCTACG 
CCACCTGAAG TCATGTCTGA ACAGTCAGTG GGAGGGCCCC CTGAGCTAGA TACAGCCAGC 
AGTTCCGAAG ATGTGTTTGA TGGGCATTTG CTGGGATCCA CAGACAGCCA AGTGAAGGAA 
AAGTCAACCA TGAAAGCCAT CTTTGCAAAT TTGCTTCCAG GAAATAGCTA TAATCCTATT 
CCATTTCCTT TTGATCCAGA TAAACACTAC TTAATGTATG AACATGAACG AGTGCCCATT 
GCAGTCTGCG AGAAGGAACC CAGCTCCATC ATTGCTTTTG CTCTCAGTTG TAAAGAATAC 
CGAAATGCCT TAGAGGAATT GTCTAAAGCG ACTCAGTGGA ACAGTGCCGA AGAAGGGCTT 
CCAACAAATA GTACTTCAGA TAGCAGACCA AAGAGTAGCA GCCCTATCAG ATTACCTGAA 
ATGAGTGGAG GACAGACAAA TCGTACAACA GAAACAGAAC CACAACCAAC CAAAAAGGCT 
TCTGGAATGT TGTCCTTCTT CAGAGGGACA GCAGGGAAAA GCCCCGATCT CTCTTCCCAG 
AAGAGAGAGA CCTTACGTGG AGCAGATAGT GCTTACTACC AGGTTGGGCA GACGGGCAAG 
GAGGGGACCG AGAATCAAGG CGTTGAGCCT CAAGATGAAG TAGATGGAGG AGATACACAA 
AAGAAGCAAC TCATAAATCC TCATGTGGAA CTTCAATTTT CAGATGCTAA TGCCAAGTTT 
TACTGTCGGC TCTACTATGC GGGAGAGTTT CATAAGATGC GTGAAGTGAT TCTGGACAGC 
AGTGAAGAAG ATTTCATTCG TTCCCTCTCC CACTCATCAC CCTGGCAGGC CCGGGGAGGC 
AAATCAGGAG CTGCCTTCTA TGCAACTGAG GATGATAGAT TTATTTTGAA GCAAATGCCT 
CGTCTGGAAG TCCAGTCCTT CCTCGACTTT GCACCACATT ACTTCAATTA TATTACAAAT 
GCTGTTCAAC AAAAGAGGCC CACGGCGTTG GCCAAAATTC TTGGAGTTTA CAGAATTGGT 
TATAAGAACT CTCAGAACAA CACTGAGAAG AAGTTAGATC TCCTTGTCAT GGAAAATCTT 
TTCTACGGGA GAAAGATGGC ACAGGTTTTT GATTTGAAGG GCTCTCTTAG GAATCGGAAT 
GTAAAAACTG ACACTGGAAA AGAGAGTTGT GATGTGGTCC TGCTAGATGA AAATCTCCTA 
AAGATGGTTC GAGACAACCC TCTATATATT CGTTCTCATT CCAAAGCTGT GCTGAGAACC 
TCGATCCATA GTGACTCCCA TTTCCTTTCT AGCCACCTCA TTATAGATTA TTCTTTGCTG 
GTTGGGCGAG ATGATACTAG CAATGAGCTA GTAGTTGGAA TTATAGATTA TATTCGAACA 
TTTACATGGG ACAAAAAGCT TGAGATGGTT GTGAAATCAA CAGGAATTTT AGGTGGACAA 
GGTAAAATGC CAACAGTGGT GTCTCCGGAG TTGTACAGGA CTAGGTTTTG TGAGGCAATG 
GACAAGTATT TCCTAATGGT ACCAGACCAC TGGACAGGCT TGGGTCTGAA TTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_055855.2
CDS	159..6455
Translation	MATDDKTSPTLDSANDLPRSPTSPSHLTHFKPLTPDQDEPPFKSAYSSFVNLFRFNKERAEGGQGEQQPLSGSWTSPQLPSRTQSVRSPTPYKKQLNEELQRRSSALDTRRKAEPTFGGHDPRTAVQLRSLSTVLKRLKEIMEGKSQDSDLKQYWMPDSQCKECYDCSEKFTTFRRRHHCRLCGQIFCSRCCNQEIPGKFMGYTGDLRACTYCRKIALSYAHSTDSNSIGEDLNALSDSACSVSVLDPSEPRTPVGSRKASRNIFLEDDLAWQSLIHPDSSNTPLSTRLVSVQEDAGKSPARNRSASITNLSLDRSGSPMVPSYETSVSPQANRTYVRTETTEDERKILLDSVQLKDLWKKICHHSSGMEFQDHRYWLRTHPNCIVGKELVNWLIRNGHIATRAQAIAIGQAMVDGRWLDCVSHHDQLFRDEYALYRPLQSTEFSETPSPDSDSVNSVEGHSEPSWFKDIKFDDSDTEQIAEEGDDNLANSASPSKRTSVSSFQSTVDSDSAASISLNVELDNVNFHIKKPSKYPHVPPHPADQKEYLISDTGGQQLSISDAFIKESLFNRRVEEKSKELPFTPLGWHHNNLELLREENGEKQAMERLLSANHNHMMALLQQLLHSDSLSSSWRDIIVSLVCQVVQTVRPDVKNQDDDMDIRQFVHIKKIPGGKKFDSVVVNGFVCTKNIAHKKMSSCIKNPKILLLKCSIEYLYREETKFTCIDPIVLQEREFLKNYVQRIVDVRPTLVLVEKTVSRIAQDMLLEHGITLVINVKSQVLERISRMTQGDLVMSMDQLLTKPHLGTCHKFYMQIFQLPNEQTKTLMFFEGCPQHLGCTIKLRGGSDYELARVKEILIFMICVAYHSQLEISFLMDEFAMPPTLMQNPSFHSLIEGRGHEGAVQEQYGGGSIPWDPDIPPESLPCDDSSLLELRIVFEKGEQENKNLPQAVASVKHQEHSTTACPAGLPCAFFAPVPESLLPLPVDDQQDALGSEQPETLQQTVVLQDPKSQIRAFRDPLQDDTGLYVTEEVTSSEDKRKTYSLAFKQELKDVILCISPVITFREPFLLTEKGMRCSTRDYFAEQVYWSPLLNKEFKEMENRRKKQLLRDLSGLQGMNGSIQAKSIQVLPSHELVSTRIAEHLGDSQSLGRMLADYRARGGRIQPKNSDPFAHSKDASSTSSGQSGSKNEGDEERGLILSDAVWSTKVDCLNPINHQRLCVLFSSSSAQSSNAPSACVSPWIVTMEFYGKNDLTLGIFLERYCFRPSYQCPSMFCDTPMVHHIRRFVHGQGCVQIILKELDSPVPGYQHTILTYSWCRICKQVTPVVALSNESWSMSFAKYLELRFYGHQYTRRANAEPCGHSIHHDYHQYFSYNQMVASFSYSPIRLLEVCVPLPKIFIKRQAPLKVSLLQDLKDFFQKVSQVYVAIDERLASLKTDTFSKTREEKMEDIFAQKEMEEGEFKNWIEKMQARLMSSSVDTPQQLQSVFESLIAKKQSLCEVLQAWNNRLQDLFQQEKGRKRPSVPPSPGRLRQGEESKISAMDASPRNISPGLQNGEKEDRFLTTLSSQSSTSSTHLQLPTPPEVMSEQSVGGPPELDTASSSEDVFDGHLLGSTDSQVKEKSTMKAIFANLLPGNSYNPIPFPFDPDKHYLMYEHERVPIAVCEKEPSSIIAFALSCKEYRNALEELSKATQWNSAEEGLPTNSTSDSRPKSSSPIRLPEMSGGQTNRTTETEPQPTKKASGMLSFFRGTAGKSPDLSSQKRETLRGADSAYYQVGQTGKEGTENQGVEPQDEVDGGDTQKKQLINPHVELQFSDANAKFYCRLYYAGEFHKMREVILDSSEEDFIRSLSHSSPWQARGGKSGAAFYATEDDRFILKQMPRLEVQSFLDFAPHYFNYITNAVQQKRPTALAKILGVYRIGYKNSQNNTEKKLDLLVMENLFYGRKMAQVFDLKGSLRNRNVKTDTGKESCDVVLLDENLLKMVRDNPLYIRSHSKAVLRTSIHSDSHFLSSHLIIDYSLLVGRDDTSNELVVGIIDYIRTFTWDKKLEMVVKSTGILGGQGKMPTVVSPELYRTRFCEAMDKYFLMVPDHWTGLGLNC

Target ORF information:

RefSeq Version	NM_015040.3
Organism	Homo sapiens(human)
Definition	Homo sapiens phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_015040.3

ORF Insert Sequence:

ATGGCCACAG ATGATAAGAC GTCCCCAACA CTGGACTCTG CTAATGATTT GCCTCGATCT 
CCTACTAGTC CTTCTCATCT CACACACTTT AAACCTTTGA CTCCTGATCA AGATGAGCCC 
CCTTTTAAAT CAGCTTATAG TTCTTTTGTA AATCTCTTTC GTTTTAACAA AGAGAGAGCA 
GAAGGAGGCC AGGGAGAACA GCAGCCTTTG AGTGGAAGTT GGACCAGCCC TCAGCTCCCT 
TCGAGGACAC AGTCTGTTAG GTCACCCACA CCTTATAAAA AGCAGCTTAA TGAGGAACTC 
CAGCGGCGCT CTTCAGCATT AGACACAAGA AGGAAAGCAG AACCTACCTT TGGAGGTCAT 
GACCCTCGTA CAGCTGTTCA GCTTCGAAGC CTCAGCACAG TATTAAAACG CCTCAAGGAA 
ATCATGGAGG GGAAAAGCCA GGATAGTGAC CTGAAACAAT ACTGGATGCC AGATAGCCAA 
TGTAAAGAGT GCTATGACTG TAGTGAGAAA TTTACAACCT TTAGGCGCAG ACACCATTGC 
CGACTATGTG GGCAGATTTT CTGCAGTCGT TGCTGTAATC AAGAAATCCC TGGAAAATTT 
ATGGGCTATA CAGGAGACCT CCGAGCTTGC ACATATTGTA GAAAAATAGC CTTAAGTTAT 
GCTCATTCCA CAGACAGTAA TTCTATTGGG GAAGACTTGA ATGCTCTTTC AGATTCTGCT 
TGCTCTGTGT CTGTGCTTGA TCCAAGTGAA CCCCGAACAC CTGTTGGGAG TAGGAAAGCC 
AGCCGTAACA TATTTTTAGA GGATGATTTG GCCTGGCAAA GTTTGATTCA TCCAGATTCC 
TCAAATACTC CTCTTTCAAC AAGACTTGTA TCTGTGCAAG AGGATGCTGG GAAATCTCCT 
GCTCGAAATA GATCAGCCAG CATTACTAAC CTGTCACTGG ATAGATCTGG TTCTCCTATG 
GTACCTTCAT ATGAGACATC TGTCAGTCCC CAGGCTAACC GAACATATGT TAGGACAGAG 
ACCACTGAGG ATGAACGCAA AATTCTTCTG GACAGTGTGC AGTTAAAAGA CCTGTGGAAA 
AAAATCTGCC ATCACAGCAG TGGAATGGAG TTTCAGGATC ACCGCTACTG GTTGAGAACG 
CATCCCAACT GCATTGTAGG AAAGGAATTA GTCAACTGGC TAATCCGAAA TGGGCATATT 
GCCACAAGGG CACAAGCTAT AGCAATTGGA CAAGCAATGG TTGATGGACG TTGGCTGGAT 
TGTGTTAGTC ATCACGACCA GCTTTTCAGA GATGAGTATG CGCTGTATAG ACCACTGCAG 
AGTACAGAAT TTTCTGAGAC GCCTTCTCCC GACAGTGACT CAGTGAACTC CGTGGAAGGA 
CACTCTGAGC CATCCTGGTT TAAAGACATA AAGTTTGATG ACAGTGACAC AGAACAGATA 
GCTGAAGAAG GTGACGATAA TTTGGCTAAT TCTGCCAGTC CTAGCAAGCG CACATCAGTC 
AGCAGTTTCC AGTCCACAGT GGACAGTGAC TCAGCCGCTT CTATCAGCCT GAACGTGGAG 
CTGGACAACG TGAACTTCCA TATCAAGAAG CCCTCCAAGT ACCCACATGT GCCCCCTCAC 
CCTGCTGACC AAAAAGAGTA TTTGATTTCT GACACTGGAG GACAACAGCT CTCAATAAGT 
GACGCTTTCA TCAAAGAATC CTTATTTAAT CGCCGAGTAG AGGAAAAATC CAAAGAGCTG 
CCTTTCACAC CTTTGGGCTG GCATCATAAC AACCTGGAGC TCCTGAGGGA GGAGAATGGG 
GAGAAACAAG CCATGGAGAG GTTGCTTTCA GCTAATCATA ACCACATGAT GGCACTACTC 
CAGCAGTTGC TCCATAGTGA CTCACTGTCA TCATCTTGGA GGGACATCAT CGTGTCATTG 
GTCTGCCAGG TTGTTCAGAC AGTCCGACCT GATGTCAAGA ACCAGGATGA TGACATGGAT 
ATCCGTCAGT TTGTCCACAT CAAAAAAATC CCAGGTGGAA AGAAGTTTGA TTCTGTGGTT 
GTCAATGGCT TTGTTTGTAC CAAGAACATT GCACATAAAA AGATGAGTTC TTGTATTAAA 
AACCCCAAAA TTCTTCTGTT GAAGTGTTCC ATTGAGTATC TCTACAGAGA AGAAACTAAG 
TTTACTTGCA TTGATCCTAT TGTGCTTCAG GAAAGGGAAT TCTTGAAGAA TTATGTCCAG 
CGAATAGTTG ATGTTCGACC CACCTTGGTT CTTGTTGAGA AAACAGTGTC TCGGATTGCC 
CAGGACATGT TATTGGAACA TGGCATTACT TTGGTCATTA ATGTAAAGTC ACAAGTTTTG 
GAACGAATCA GTCGAATGAC CCAAGGTGAT TTAGTGATGT CAATGGACCA GCTGCTTACG 
AAACCACACC TGGGCACTTG TCACAAATTT TATATGCAGA TATTTCAGTT GCCTAATGAA 
CAAACCAAGA CACTGATGTT TTTTGAAGGT TGTCCACAGC ACCTAGGCTG TACAATCAAG 
CTAAGAGGAG GCTCTGATTA TGAGCTGGCT CGAGTTAAGG AGATCCTAAT ATTTATGATC 
TGTGTTGCTT ATCATTCTCA ACTAGAAATA TCCTTTCTCA TGGATGAATT TGCTATGCCT 
CCCACATTAA TGCAAAACCC TTCATTCCAT TCCCTGATTG AGGGACGAGG GCATGAGGGG 
GCTGTCCAAG AGCAGTACGG TGGAGGTTCC ATCCCCTGGG ATCCTGACAT CCCTCCTGAG 
TCTCTGCCCT GTGATGATAG CAGTTTGCTG GAATTGAGGA TTGTGTTTGA GAAGGGTGAG 
CAGGAAAATA AAAATCTTCC GCAGGCTGTT GCCTCTGTGA AGCATCAAGA ACATAGCACA 
ACAGCTTGCC CGGCGGGTCT CCCTTGTGCT TTCTTTGCAC CTGTACCGGA ATCATTGTTG 
CCACTCCCTG TGGATGACCA ACAAGATGCT TTAGGCAGCG AGCAGCCAGA GACTTTGCAG 
CAAACAGTTG TGCTGCAGGA TCCCAAAAGC CAGATAAGAG CCTTTAGAGA CCCTCTACAG 
GATGACACTG GATTATATGT TACTGAGGAA GTCACCTCCT CTGAAGATAA ACGAAAGACT 
TATTCTTTGG CCTTTAAGCA GGAATTAAAA GATGTGATCC TCTGTATCTC CCCAGTAATC 
ACATTCCGAG AACCCTTTCT TTTAACTGAA AAGGGGATGA GATGCTCTAC CCGAGATTAT 
TTTGCAGAGC AGGTTTACTG GTCTCCTCTC CTCAATAAAG AATTCAAAGA AATGGAGAAC 
AGGAGGAAGA AACAGCTGCT CAGGGATCTC TCTGGACTTC AGGGCATGAA TGGAAGTATT 
CAGGCCAAGT CTATTCAAGT CTTACCCTCA CATGAGCTAG TGAGCACTAG AATTGCTGAG 
CATCTGGGCG ATAGCCAGAG CTTGGGTAGA ATGCTGGCCG ATTATCGAGC CAGAGGAGGA 
AGAATTCAGC CCAAAAATTC AGACCCTTTT GCTCATTCAA AGGATGCATC AAGTACTTCA 
AGTGGCCAAT CAGGAAGCAA AAATGAGGGT GATGAAGAGA GAGGGCTTAT TCTGAGTGAT 
GCTGTGTGGT CAACAAAGGT GGACTGTCTG AATCCCATTA ATCACCAGAG ACTTTGTGTG 
CTCTTCAGCA GCTCTTCTGC CCAGTCCAGC AATGCTCCTA GTGCCTGTGT CAGTCCTTGG 
ATTGTAACAA TGGAATTTTA TGGAAAGAAT GATCTTACAT TAGGAATATT TTTAGAGAGA 
TACTGTTTCA GGCCTTCTTA TCAGTGTCCA AGCATGTTCT GTGATACCCC CATGGTACAT 
CATATTCGGC GCTTTGTTCA TGGCCAAGGC TGTGTGCAGA TAATCCTGAA GGAGTTGGAT 
TCTCCAGTAC CTGGATATCA GCATACAATT CTTACATATT CCTGGTGTAG AATCTGCAAA 
CAGGTAACAC CAGTTGTTGC TCTTTCCAAT GAGTCCTGGT CTATGTCATT TGCAAAATAC 
CTTGAACTTA GGTTTTATGG GCACCAGTAT ACTCGCAGAG CCAACGCTGA GCCCTGTGGT 
CACTCCATCC ATCATGATTA TCACCAGTAT TTCTCCTATA ACCAGATGGT GGCGTCTTTC 
AGTTATTCTC CCATTCGGCT TCTTGAAGTA TGTGTTCCAC TCCCCAAAAT ATTCATTAAG 
CGTCAGGCCC CATTAAAAGT GTCCCTTCTT CAGGATCTGA AGGACTTCTT TCAAAAAGTT 
TCACAGGTAT ATGTTGCCAT TGATGAAAGA CTTGCATCTT TGAAAACTGA TACATTTAGT 
AAAACAAGAG AGGAAAAAAT GGAAGATATT TTTGCACAGA AAGAGATGGA AGAAGGTGAG 
TTCAAGAACT GGATTGAGAA GATGCAAGCA AGGCTCATGT CTTCCTCTGT AGATACCCCT 
CAGCAACTGC AGTCGGTCTT TGAGTCACTC ATTGCCAAGA AACAAAGTCT CTGTGAAGTG 
CTGCAAGCTT GGAATAACAG GTTGCAGGAC CTTTTCCAAC AGGAAAAGGG TAGAAAGAGA 
CCTTCAGTTC CTCCAAGTCC TGGAAGACTG AGACAAGGGG AAGAAAGCAA GATAAGTGCG 
ATGGATGCAT CTCCACGGAA TATTTCTCCA GGACTTCAGA ATGGAGAAAA AGAGGATCGC 
TTCTTAACAA CTTTGTCCAG CCAGAGCTCC ACCAGTTCTA CTCATCTCCA ATTGCCTACG 
CCACCTGAAG TCATGTCTGA ACAGTCAGTG GGAGGGCCCC CTGAGCTAGA TACAGCCAGC 
AGTTCCGAAG ATGTGTTTGA TGGGCATTTG CTGGGATCCA CAGACAGCCA AGTGAAGGAA 
AAGTCAACCA TGAAAGCCAT CTTTGCAAAT TTGCTTCCAG GAAATAGCTA TAATCCTATT 
CCATTTCCTT TTGATCCAGA TAAACACTAC TTAATGTATG AACATGAACG AGTGCCCATT 
GCAGTCTGCG AGAAGGAACC CAGCTCCATC ATTGCTTTTG CTCTCAGTTG TAAAGAATAC 
CGAAATGCCT TAGAGGAATT GTCTAAAGCG ACTCAGTGGA ACAGTGCCGA AGAAGGGCTT 
CCAACAAATA GTACTTCAGA TAGCAGACCA AAGAGTAGCA GCCCTATCAG ATTACCTGAA 
ATGAGTGGAG GACAGACAAA TCGTACAACA GAAACAGAAC CACAACCAAC CAAAAAGGCT 
TCTGGAATGT TGTCCTTCTT CAGAGGGACA GCAGGGAAAA GCCCCGATCT CTCTTCCCAG 
AAGAGAGAGA CCTTACGTGG AGCAGATAGT GCTTACTACC AGGTTGGGCA GACGGGCAAG 
GAGGGGACCG AGAATCAAGG CGTTGAGCCT CAAGATGAAG TAGATGGAGG AGATACACAA 
AAGAAGCAAC TCATAAATCC TCATGTGGAA CTTCAATTTT CAGATGCTAA TGCCAAGTTT 
TACTGTCGGC TCTACTATGC GGGAGAGTTT CATAAGATGC GTGAAGTGAT TCTGGACAGC 
AGTGAAGAAG ATTTCATTCG TTCCCTCTCC CACTCATCAC CCTGGCAGGC CCGGGGAGGC 
AAATCAGGAG CTGCCTTCTA TGCAACTGAG GATGATAGAT TTATTTTGAA GCAAATGCCT 
CGTCTGGAAG TCCAGTCCTT CCTCGACTTT GCACCACATT ACTTCAATTA TATTACAAAT 
GCTGTTCAAC AAAAGAGGCC CACGGCGTTG GCCAAAATTC TTGGAGTTTA CAGAATTGGT 
TATAAGAACT CTCAGAACAA CACTGAGAAG AAGTTAGATC TCCTTGTCAT GGAAAATCTT 
TTCTACGGGA GAAAGATGGC ACAGGTTTTT GATTTGAAGG GCTCTCTTAG GAATCGGAAT 
GTAAAAACTG ACACTGGAAA AGAGAGTTGT GATGTGGTCC TGCTAGATGA AAATCTCCTA 
AAGATGGTTC GAGACAACCC TCTATATATT CGTTCTCATT CCAAAGCTGT GCTGAGAACC 
TCGATCCATA GTGACTCCCA TTTCCTTTCT AGCCACCTCA TTATAGATTA TTCTTTGCTG 
GTTGGGCGAG ATGATACTAG CAATGAGCTA GTAGTTGGAA TTATAGATTA TATTCGAACA 
TTTACATGGG ACAAAAAGCT TGAGATGGTT GTGAAATCAA CAGGAATTTT AGGTGGACAA 
GGTAAAATGC CAACAGTGGT GTCTCCGGAG TTGTACAGGA CTAGGTTTTG TGAGGCAATG 
GACAAGTATT TCCTAATGGT ACCAGACCAC TGGACAGGCT TGGGTCTGAA TTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PIKFYVE ( NM_001178000.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001171471.1 Homo sapiens phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant 4, mRNA.

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CloneID	OHu09478
Clone ID Related Accession (Same CDS sequence)	NM_001178000.1
Accession Version	NM_001178000.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1647bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Homo sapiens(human)
Product	1-phosphatidylinositol 3-phosphate 5-kinase isoform 4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC032389.1 and BC125053.1. Transcript Variant: This variant (4) lacks all of the 3' coding region and UTR, compared to variant 2. The encoded protein (isoform 4) has an identical N-terminus but a short novel C-terminus, compared to isoform 2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC125053.1, SRR1163657.264233.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCACAG ATGATAAGAC GTCCCCAACA CTGGACTCTG CTAATGATTT GCCTCGATCT 
CCTACTAGTC CTTCTCATCT CACACACTTT AAACCTTTGA CTCCTGATCA AGATGAGCCC 
CCTTTTAAAT CAGCTTATAG TTCTTTTGTA AATCTCTTTC GTTTTAACAA AGAGAGAGCA 
GAAGGAGGCC AGGGAGAACA GCAGCCTTTG AGTGGAAGTT GGACCAGCCC TCAGCTCCCT 
TCGAGGACAC AGTCTGTTAG GTCACCCACA CCTTATAAAA AGCAGCTTAA TGAGGAACTC 
CAGCGGCGCT CTTCAGCATT AGACACAAGA AGGAAAGCAG AACCTACCTT TGGAGGTCAT 
GACCCTCGTA CAGCTGTTCA GCTTCGAAGC CTCAGCACAG TATTAAAACG CCTCAAGGAA 
ATCATGGAGG GGAAAAGCCA GGATAGTGAC CTGAAACAAT ACTGGATGCC AGATAGCCAA 
TGTAAAGAGT GCTATGACTG TAGTGAGAAA TTTACAACCT TTAGGCGCAG ACACCATTGC 
CGACTATGTG GGCAGATTTT CTGCAGTCGT TGCTGTAATC AAGAAATCCC TGGAAAATTT 
ATGGGCTATA CAGGAGACCT CCGAGCTTGC ACATATTGTA GAAAAATAGC CTTAAGTTAT 
GCTCATTCCA CAGACAGTAA TTCTATTGGG GAAGACTTGA ATGCTCTTTC AGATTCTGCT 
TGCTCTGTGT CTGTGCTTGA TCCAAGTGAA CCCCGAACAC CTGTTGGGAG TAGGAAAGCC 
AGCCGTAACA TATTTTTAGA GGATGATTTG GCCTGGCAAA GTTTGATTCA TCCAGATTCC 
TCAAATACTC CTCTTTCAAC AAGACTTGTA TCTGTGCAAG AGGATGCTGG GAAATCTCCT 
GCTCGAAATA GATCAGCCAG CATTACTAAC CTGTCACTGG ATAGATCTGG TTCTCCTATG 
GTACCTTCAT ATGAGACATC TGTCAGTCCC CAGGCTAACC GAACATATGT TAGGACAGAG 
ACCACTGAGG ATGAACGCAA AATTCTTCTG GACAGTGTGC AGTTAAAAGA CCTGTGGAAA 
AAAATCTGCC ATCACAGCAG TGGAATGGAG TTTCAGGATC ACCGCTACTG GTTGAGAACG 
CATCCCAACT GCATTGTAGG AAAGGAATTA GTCAACTGGC TAATCCGAAA TGGGCATATT 
GCCACAAGGG CACAAGCTAT AGCAATTGGA CAAGCAATGG TTGATGGACG TTGGCTGGAT 
TGTGTTAGTC ATCACGACCA GCTTTTCAGA GATGAGTATG CGCTGTATAG ACCACTGCAG 
AGTACAGAAT TTTCTGAGAC GCCTTCTCCC GACAGTGACT CAGTGAACTC CGTGGAAGGA 
CACTCTGAGC CATCCTGGTT TAAAGACATA AAGTTTGATG ACAGTGACAC AGAACAGATA 
GCTGAAGAAG GTGACGATAA TTTGGCTAAT TCTGCCAGTC CTAGCAAGCG CACATCAGTC 
AGCAGTTTCC AGTCCACAGT GGACAGTGAC TCAGCCGCTT CTATCAGCCT GAACGTGGAG 
CTGGACAACG TGAACTTCCA TATCAAGAAG CCCTCCAAGT ACCCACATGT GCCCCCTCAC 
CCTGCTGACC AAAAAGGTAG GAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001171471.1
CDS	159..1805
Translation	MATDDKTSPTLDSANDLPRSPTSPSHLTHFKPLTPDQDEPPFKSAYSSFVNLFRFNKERAEGGQGEQQPLSGSWTSPQLPSRTQSVRSPTPYKKQLNEELQRRSSALDTRRKAEPTFGGHDPRTAVQLRSLSTVLKRLKEIMEGKSQDSDLKQYWMPDSQCKECYDCSEKFTTFRRRHHCRLCGQIFCSRCCNQEIPGKFMGYTGDLRACTYCRKIALSYAHSTDSNSIGEDLNALSDSACSVSVLDPSEPRTPVGSRKASRNIFLEDDLAWQSLIHPDSSNTPLSTRLVSVQEDAGKSPARNRSASITNLSLDRSGSPMVPSYETSVSPQANRTYVRTETTEDERKILLDSVQLKDLWKKICHHSSGMEFQDHRYWLRTHPNCIVGKELVNWLIRNGHIATRAQAIAIGQAMVDGRWLDCVSHHDQLFRDEYALYRPLQSTEFSETPSPDSDSVNSVEGHSEPSWFKDIKFDDSDTEQIAEEGDDNLANSASPSKRTSVSSFQSTVDSDSAASISLNVELDNVNFHIKKPSKYPHVPPHPADQKGRR

Target ORF information:

RefSeq Version	NM_001178000.1
Organism	Homo sapiens(human)
Definition	Homo sapiens phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001178000.1

ORF Insert Sequence:

ATGGCCACAG ATGATAAGAC GTCCCCAACA CTGGACTCTG CTAATGATTT GCCTCGATCT 
CCTACTAGTC CTTCTCATCT CACACACTTT AAACCTTTGA CTCCTGATCA AGATGAGCCC 
CCTTTTAAAT CAGCTTATAG TTCTTTTGTA AATCTCTTTC GTTTTAACAA AGAGAGAGCA 
GAAGGAGGCC AGGGAGAACA GCAGCCTTTG AGTGGAAGTT GGACCAGCCC TCAGCTCCCT 
TCGAGGACAC AGTCTGTTAG GTCACCCACA CCTTATAAAA AGCAGCTTAA TGAGGAACTC 
CAGCGGCGCT CTTCAGCATT AGACACAAGA AGGAAAGCAG AACCTACCTT TGGAGGTCAT 
GACCCTCGTA CAGCTGTTCA GCTTCGAAGC CTCAGCACAG TATTAAAACG CCTCAAGGAA 
ATCATGGAGG GGAAAAGCCA GGATAGTGAC CTGAAACAAT ACTGGATGCC AGATAGCCAA 
TGTAAAGAGT GCTATGACTG TAGTGAGAAA TTTACAACCT TTAGGCGCAG ACACCATTGC 
CGACTATGTG GGCAGATTTT CTGCAGTCGT TGCTGTAATC AAGAAATCCC TGGAAAATTT 
ATGGGCTATA CAGGAGACCT CCGAGCTTGC ACATATTGTA GAAAAATAGC CTTAAGTTAT 
GCTCATTCCA CAGACAGTAA TTCTATTGGG GAAGACTTGA ATGCTCTTTC AGATTCTGCT 
TGCTCTGTGT CTGTGCTTGA TCCAAGTGAA CCCCGAACAC CTGTTGGGAG TAGGAAAGCC 
AGCCGTAACA TATTTTTAGA GGATGATTTG GCCTGGCAAA GTTTGATTCA TCCAGATTCC 
TCAAATACTC CTCTTTCAAC AAGACTTGTA TCTGTGCAAG AGGATGCTGG GAAATCTCCT 
GCTCGAAATA GATCAGCCAG CATTACTAAC CTGTCACTGG ATAGATCTGG TTCTCCTATG 
GTACCTTCAT ATGAGACATC TGTCAGTCCC CAGGCTAACC GAACATATGT TAGGACAGAG 
ACCACTGAGG ATGAACGCAA AATTCTTCTG GACAGTGTGC AGTTAAAAGA CCTGTGGAAA 
AAAATCTGCC ATCACAGCAG TGGAATGGAG TTTCAGGATC ACCGCTACTG GTTGAGAACG 
CATCCCAACT GCATTGTAGG AAAGGAATTA GTCAACTGGC TAATCCGAAA TGGGCATATT 
GCCACAAGGG CACAAGCTAT AGCAATTGGA CAAGCAATGG TTGATGGACG TTGGCTGGAT 
TGTGTTAGTC ATCACGACCA GCTTTTCAGA GATGAGTATG CGCTGTATAG ACCACTGCAG 
AGTACAGAAT TTTCTGAGAC GCCTTCTCCC GACAGTGACT CAGTGAACTC CGTGGAAGGA 
CACTCTGAGC CATCCTGGTT TAAAGACATA AAGTTTGATG ACAGTGACAC AGAACAGATA 
GCTGAAGAAG GTGACGATAA TTTGGCTAAT TCTGCCAGTC CTAGCAAGCG CACATCAGTC 
AGCAGTTTCC AGTCCACAGT GGACAGTGAC TCAGCCGCTT CTATCAGCCT GAACGTGGAG 
CTGGACAACG TGAACTTCCA TATCAAGAAG CCCTCCAAGT ACCCACATGT GCCCCCTCAC 
CCTGCTGACC AAAAAGGTAG GAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PIKFYVE ( XM_017003570.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016859059.1 Homo sapiens phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X15, mRNA.

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CloneID	OHu89145
Clone ID Related Accession (Same CDS sequence)	XM_017003570.1
Accession Version	XM_017003570.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5838bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	1-phosphatidylinositol 3-phosphate 5-kinase isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG ATGATAAGAC GTCCCCAACA CTGGACTCTG CTAATGATTT GCCTCGATCT 
CCTACTAGTC CTTCTCATCT CACACACTTT AAACCTTTGA CTCCTGATCA AGATGAGCCC 
CCTTTTAAAT CAGCTTATAG TTCTTTTGTA AATCTCTTTC GTTTTAACAA AGAGAGAGCA 
GAAGGAGGCC AGGGAGAACA GCAGCCTTTG AGTGGAAGTT GGACCAGCCC TCAGCTCCCT 
TCGAGGACAC AGTCTGTTAG GTCACCCACA CCTTATAAAA AGCAGCTTAA TGAGGAACTC 
CAGCGGCGCT CTTCAGCATT AGGAGACCTC CGAGCTTGCA CATATTGTAG AAAAATAGCC 
TTAAGTTATG CTCATTCCAC AGACAGTAAT TCTATTGGGG AAGACTTGAA TGCTCTTTCA 
GATTCTGCTT GCTCTGTGTC TGTGCTTGAT CCAAGTGAAC CCCGAACACC TGTTGGGAGT 
AGGAAAGCCA GCCGTAACAT ATTTTTAGAG GATGATTTGG CCTGGCAAAG TTTGATTCAT 
CCAGATTCCT CAAATACTCC TCTTTCAACA AGACTTGTAT CTGTGCAAGA GGATGCTGGG 
AAATCTCCTG CTCGAAATAG ATCAGCCAGC ATTACTAACC TGTCACTGGA TAGATCTGGT 
TCTCCTATGG TACCTTCATA TGAGACATCT GTCAGTCCCC AGGCTAACCG AACATATGTT 
AGGACAGAGA CCACTGAGGA TGAACGCAAA ATTCTTCTGG ACAGTGTGCA GTTAAAAGAC 
CTGTGGAAAA AAATCTGCCA TCACAGCAGT GGAATGGAGT TTCAGGATCA CCGCTACTGG 
TTGAGAACGC ATCCCAACTG CATTGTAGGA AAGGAATTAG TCAACTGGCT AATCCGAAAT 
GGGCATATTG CCACAAGGGC ACAAGCTATA GCAATTGGAC AAGCAATGGT TGATGGACGT 
TGGCTGGATT GTGTTAGTCA TCACGACCAG CTTTTCAGAG ATGAGTATGC GCTGTATAGA 
CCACTGCAGA GTACAGAATT TTCTGAGACG CCTTCTCCCG ACAGTGACTC AGTGAACTCC 
GTGGAAGGAC ACTCTGAGCC ATCCTGGTTT AAAGACATAA AGTTTGATGA CAGTGACACA 
GAACAGATAG CTGAAGAAGG TGACGATAAT TTGGCTAAGT ATTTGATTTC TGACACTGGA 
GGACAACAGC TCTCAATAAG TGACGCTTTC ATCAAAGAAT CCTTATTTAA TCGCCGAGTA 
GAGGAAAAAT CCAAAGAGCT GCCTTTCACA CCTTTGGGCT GGCATCATAA CAACCTGGAG 
CTCCTGAGGG AGGAGAATGG GGAGAAACAA GCCATGGAGA GGTTGCTTTC AGCTAATCAT 
AACCACATGA TGGCACTACT CCAGCAGTTG CTCCATAGTG ACTCACTGTC ATCATCTTGG 
AGGGACATCA TCGTGTCATT GGTCTGCCAG GTTGTTCAGA CAGTCCGACC TGATGTCAAG 
AACCAGGATG ATGACATGGA TATCCGTCAG TTTGTCCACA TCAAAAAAAT CCCAGGTGGA 
AAGAAGTTTG ATTCTGTGGT TGTCAATGGC TTTGTTTGTA CCAAGAACAT TGCACATAAA 
AAGATGAGTT CTTGTATTAA AAACCCCAAA ATTCTTCTGT TGAAGTGTTC CATTGAGTAT 
CTCTACAGAG AAGAAACTAA GTTTACTTGC ATTGATCCTA TTGTGCTTCA GGAAAGGGAA 
TTCTTGAAGA ATTATGTCCA GCGAATAGTT GATGTTCGAC CCACCTTGGT TCTTGTTGAG 
AAAACAGTGT CTCGGATTGC CCAGGACATG TTATTGGAAC ATGGCATTAC TTTGGTCATT 
AATGTAAAGT CACAAGTTTT GGAACGAATC AGTCGAATGA CCCAAGGTGA TTTAGTGATG 
TCAATGGACC AGCTGCTTAC GAAACCACAC CTGGGCACTT GTCACAAATT TTATATGCAG 
ATATTTCAGT TGCCTAATGA ACAAACCAAG ACACTGATGT TTTTTGAAGG TTGTCCACAG 
CACCTAGGCT GTACAATCAA GCTAAGAGGA GGCTCTGATT ATGAGCTGGC TCGAGTTAAG 
GAGATCCTAA TATTTATGAT CTGTGTTGCT TATCATTCTC AACTAGAAAT ATCCTTTCTC 
ATGGATGAAT TTGCTATGCC TCCCACATTA ATGCAAAACC CTTCATTCCA TTCCCTGATT 
GAGGGACGAG GGCATGAGGG GGCTGTCCAA GAGCAGTACG GTGGAGGTTC CATCCCCTGG 
GATCCTGACA TCCCTCCTGA GTCTCTGCCC TGTGATGATA GCAGTTTGCT GGAATTGAGG 
ATTGTGTTTG AGAAGGGTGA GCAGGAAAAT AAAAATCTTC CGCAGGCTGT TGCCTCTGTG 
AAGCATCAAG AACATAGCAC AACAGCTTGC CCGGCGGGTC TCCCTTGTGC TTTCTTTGCA 
CCTGTACCGG AATCATTGTT GCCACTCCCT GTGGATGACC AACAAGATGC TTTAGGCAGC 
GAGCAGCCAG AGACTTTGCA GCAAACAGTT GTGCTGCAGG ATCCCAAAAG CCAGATAAGA 
GCCTTTAGAG ACCCTCTACA GGATGACACT GGATTATATG TTACTGAGGA AGTCACCTCC 
TCTGAAGATA AACGAAAGAC TTATTCTTTG GCCTTTAAGC AGGAATTAAA AGATGTGATC 
CTCTGTATCT CCCCAGTAAT CACATTCCGA GAACCCTTTC TTTTAACTGA AAAGGGGATG 
AGATGCTCTA CCCGAGATTA TTTTGCAGAG CAGGTTTACT GGTCTCCTCT CCTCAATAAA 
GAATTCAAAG AAATGGAGAA CAGGAGGAAG AAACAGCTGC TCAGGGATCT CTCTGGACTT 
CAGGGCATGA ATGGAAGTAT TCAGGCCAAG TCTATTCAAG TCTTACCCTC ACATGAGCTA 
GTGAGCACTA GAATTGCTGA GCATCTGGGC GATAGCCAGA GCTTGGGTAG AATGCTGGCC 
GATTATCGAG CCAGAGGAGG AAGAATTCAG CCCAAAAATT CAGACCCTTT TGCTCATTCA 
AAGGATGCAT CAAGTACTTC AAGTGGCCAA TCAGGAAGCA AAAATGAGGG TGATGAAGAG 
AGAGGGCTTA TTCTGAGTGA TGCTGTGTGG TCAACAAAGG TGGACTGTCT GAATCCCATT 
AATCACCAGA GACTTTGTGT GCTCTTCAGC AGCTCTTCTG CCCAGTCCAG CAATGCTCCT 
AGTGCCTGTG TCAGTCCTTG GATTGTAACA ATGGAATTTT ATGGAAAGAA TGATCTTACA 
TTAGGAATAT TTTTAGAGAG ATACTGTTTC AGGCCTTCTT ATCAGTGTCC AAGCATGTTC 
TGTGATACCC CCATGGTACA TCATATTCGG CGCTTTGTTC ATGGCCAAGG CTGTGTGCAG 
ATAATCCTGA AGGAGTTGGA TTCTCCAGTA CCTGGATATC AGCATACAAT TCTTACATAT 
TCCTGGTGTA GAATCTGCAA ACAGGTAACA CCAGTTGTTG CTCTTTCCAA TGAGTCCTGG 
TCTATGTCAT TTGCAAAATA CCTTGAACTT AGGTTTTATG GGCACCAGTA TACTCGCAGA 
GCCAACGCTG AGCCCTGTGG TCACTCCATC CATCATGATT ATCACCAGTA TTTCTCCTAT 
AACCAGATGG TGGCGTCTTT CAGTTATTCT CCCATTCGGC TTCTTGAAGT ATGTGTTCCA 
CTCCCCAAAA TATTCATTAA GCGTCAGGCC CCATTAAAAG TGTCCCTTCT TCAGGATCTG 
AAGGACTTCT TTCAAAAAGT TTCACAGGTA TATGTTGCCA TTGATGAAAG ACTTGCATCT 
TTGAAAACTG ATACATTTAG TAAAACAAGA GAGGAAAAAA TGGAAGATAT TTTTGCACAG 
AAAGAGATGG AAGAAGGTGA GTTCAAGAAC TGGATTGAGA AGATGCAAGC AAGGCTCATG 
TCTTCCTCTG TAGATACCCC TCAGCAACTG CAGTCGGTCT TTGAGTCACT CATTGCCAAG 
AAACAAAGTC TCTGTGAAGT GCTGCAAGCT TGGAATAACA GGTTGCAGGA CCTTTTCCAA 
CAGGAAAAGG GTAGAAAGAG ACCTTCAGTT CCTCCAAGTC CTGGAAGACT GAGACAAGGG 
GAAGAAAGCA AGATAAGTGC GATGGATGCA TCTCCACGGA ATATTTCTCC AGGACTTCAG 
AATGGAGAAA AAGAGGATCG CTTCTTAACA ACTTTGTCCA GCCAGAGCTC CACCAGTTCT 
ACTCATCTCC AATTGCCTAC GCCACCTGAA GTCATGTCTG AACAGTCAGT GGGAGGGCCC 
CCTGAGCTAG ATACAGCCAG CAGTTCCGAA GATGTGTTTG ATGGGCATTT GCTGGGATCC 
ACAGACAGCC AAGTGAAGGA AAAGTCAACC ATGAAAGCCA TCTTTGCAAA TTTGCTTCCA 
GGAAATAGCT ATAATCCTAT TCCATTTCCT TTTGATCCAG ATAAACACTA CTTAATGTAT 
GAACATGAAC GAGTGCCCAT TGCAGTCTGC GAGAAGGAAC CCAGCTCCAT CATTGCTTTT 
GCTCTCAGTT GTAAAGAATA CCGAAATGCC TTAGAGGAAT TGTCTAAAGC GACTCAGTGG 
AACAGTGCCG AAGAAGGGCT TCCAACAAAT AGTACTTCAG ATAGCAGACC AAAGAGTAGC 
AGCCCTATCA GATTACCTGA AATGAGTGGA GGACAGACAA ATCGTACAAC AGAAACAGAA 
CCACAACCAA CCAAAAAGGC TTCTGGAATG TTGTCCTTCT TCAGAGGGAC AGCAGGGAAA 
AGCCCCGATC TCTCTTCCCA GAAGAGAGAG ACCTTACGTG GAGCAGATAG TGCTTACTAC 
CAGGTTGGGC AGACGGGCAA GGAGGGGACC GAGAATCAAG GCGTTGAGCC TCAAGATGAA 
GTAGATGGAG GAGATACACA AAAGAAGCAA CTCATAAATC CTCATGTGGA ACTTCAATTT 
TCAGATGCTA ATGCCAAGTT TTACTGTCGG CTCTACTATG CGGGAGAGTT TCATAAGATG 
CGTGAAGTGA TTCTGGACAG CAGTGAAGAA GATTTCATTC GTTCCCTCTC CCACTCATCA 
CCCTGGCAGG CCCGGGGAGG CAAATCAGGA GCTGCCTTCT ATGCAACTGA GGATGATAGA 
TTTATTTTGA AGCAAATGCC TCGTCTGGAA GTCCAGTCCT TCCTCGACTT TGCACCACAT 
TACTTCAATT ATATTACAAA TGCTGTTCAA CAAAAGAGGC CCACGGCGTT GGCCAAAATT 
CTTGGAGTTT ACAGAATTGG TTATAAGAAC TCTCAGAACA ACACTGAGAA GAAGTTAGAT 
CTCCTTGTCA TGGAAAATCT TTTCTACGGG AGAAAGATGG CACAGGTTTT TGATTTGAAG 
GGCTCTCTTA GGAATCGGAA TGTAAAAACT GACACTGGAA AAGAGAGTTG TGATGTGGTC 
CTGCTAGATG AAAATCTCCT AAAGATGGTT CGAGACAACC CTCTATATAT TCGTTCTCAT 
TCCAAAGCTG TGCTGAGAAC CTCGATCCAT AGTGACTCCC ATTTCCTTTC TAGCCACCTC 
ATTATAGATT ATTCTTTGCT GGTTGGGCGA GATGATACTA GCAATGAGCT AGTAGTTGGA 
ATTATAGATT ATATTCGAAC ATTTACATGG GACAAAAAGC TTGAGATGGT TGTGAAATCA 
ACAGGAATTT TAGGTGGACA AGGTAAAATG CCAACAGTGG TGTCTCCGGA GTTGTACAGG 
ACTAGGTTTT GTGAGGCAAT GGACAAGTAT TTCCTAATGG TACCAGACCA CTGGACAGGC 
TTGGGTCTGA ATTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016859059.1
CDS	185..6022
Translation	MATDDKTSPTLDSANDLPRSPTSPSHLTHFKPLTPDQDEPPFKSAYSSFVNLFRFNKERAEGGQGEQQPLSGSWTSPQLPSRTQSVRSPTPYKKQLNEELQRRSSALGDLRACTYCRKIALSYAHSTDSNSIGEDLNALSDSACSVSVLDPSEPRTPVGSRKASRNIFLEDDLAWQSLIHPDSSNTPLSTRLVSVQEDAGKSPARNRSASITNLSLDRSGSPMVPSYETSVSPQANRTYVRTETTEDERKILLDSVQLKDLWKKICHHSSGMEFQDHRYWLRTHPNCIVGKELVNWLIRNGHIATRAQAIAIGQAMVDGRWLDCVSHHDQLFRDEYALYRPLQSTEFSETPSPDSDSVNSVEGHSEPSWFKDIKFDDSDTEQIAEEGDDNLAKYLISDTGGQQLSISDAFIKESLFNRRVEEKSKELPFTPLGWHHNNLELLREENGEKQAMERLLSANHNHMMALLQQLLHSDSLSSSWRDIIVSLVCQVVQTVRPDVKNQDDDMDIRQFVHIKKIPGGKKFDSVVVNGFVCTKNIAHKKMSSCIKNPKILLLKCSIEYLYREETKFTCIDPIVLQEREFLKNYVQRIVDVRPTLVLVEKTVSRIAQDMLLEHGITLVINVKSQVLERISRMTQGDLVMSMDQLLTKPHLGTCHKFYMQIFQLPNEQTKTLMFFEGCPQHLGCTIKLRGGSDYELARVKEILIFMICVAYHSQLEISFLMDEFAMPPTLMQNPSFHSLIEGRGHEGAVQEQYGGGSIPWDPDIPPESLPCDDSSLLELRIVFEKGEQENKNLPQAVASVKHQEHSTTACPAGLPCAFFAPVPESLLPLPVDDQQDALGSEQPETLQQTVVLQDPKSQIRAFRDPLQDDTGLYVTEEVTSSEDKRKTYSLAFKQELKDVILCISPVITFREPFLLTEKGMRCSTRDYFAEQVYWSPLLNKEFKEMENRRKKQLLRDLSGLQGMNGSIQAKSIQVLPSHELVSTRIAEHLGDSQSLGRMLADYRARGGRIQPKNSDPFAHSKDASSTSSGQSGSKNEGDEERGLILSDAVWSTKVDCLNPINHQRLCVLFSSSSAQSSNAPSACVSPWIVTMEFYGKNDLTLGIFLERYCFRPSYQCPSMFCDTPMVHHIRRFVHGQGCVQIILKELDSPVPGYQHTILTYSWCRICKQVTPVVALSNESWSMSFAKYLELRFYGHQYTRRANAEPCGHSIHHDYHQYFSYNQMVASFSYSPIRLLEVCVPLPKIFIKRQAPLKVSLLQDLKDFFQKVSQVYVAIDERLASLKTDTFSKTREEKMEDIFAQKEMEEGEFKNWIEKMQARLMSSSVDTPQQLQSVFESLIAKKQSLCEVLQAWNNRLQDLFQQEKGRKRPSVPPSPGRLRQGEESKISAMDASPRNISPGLQNGEKEDRFLTTLSSQSSTSSTHLQLPTPPEVMSEQSVGGPPELDTASSSEDVFDGHLLGSTDSQVKEKSTMKAIFANLLPGNSYNPIPFPFDPDKHYLMYEHERVPIAVCEKEPSSIIAFALSCKEYRNALEELSKATQWNSAEEGLPTNSTSDSRPKSSSPIRLPEMSGGQTNRTTETEPQPTKKASGMLSFFRGTAGKSPDLSSQKRETLRGADSAYYQVGQTGKEGTENQGVEPQDEVDGGDTQKKQLINPHVELQFSDANAKFYCRLYYAGEFHKMREVILDSSEEDFIRSLSHSSPWQARGGKSGAAFYATEDDRFILKQMPRLEVQSFLDFAPHYFNYITNAVQQKRPTALAKILGVYRIGYKNSQNNTEKKLDLLVMENLFYGRKMAQVFDLKGSLRNRNVKTDTGKESCDVVLLDENLLKMVRDNPLYIRSHSKAVLRTSIHSDSHFLSSHLIIDYSLLVGRDDTSNELVVGIIDYIRTFTWDKKLEMVVKSTGILGGQGKMPTVVSPELYRTRFCEAMDKYFLMVPDHWTGLGLNC

Target ORF information:

RefSeq Version	XM_017003570.1
Organism	Homo sapiens(human)
Definition	Homo sapiens phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017003570.1

ORF Insert Sequence:

ATGGCCACAG ATGATAAGAC GTCCCCAACA CTGGACTCTG CTAATGATTT GCCTCGATCT 
CCTACTAGTC CTTCTCATCT CACACACTTT AAACCTTTGA CTCCTGATCA AGATGAGCCC 
CCTTTTAAAT CAGCTTATAG TTCTTTTGTA AATCTCTTTC GTTTTAACAA AGAGAGAGCA 
GAAGGAGGCC AGGGAGAACA GCAGCCTTTG AGTGGAAGTT GGACCAGCCC TCAGCTCCCT 
TCGAGGACAC AGTCTGTTAG GTCACCCACA CCTTATAAAA AGCAGCTTAA TGAGGAACTC 
CAGCGGCGCT CTTCAGCATT AGGAGACCTC CGAGCTTGCA CATATTGTAG AAAAATAGCC 
TTAAGTTATG CTCATTCCAC AGACAGTAAT TCTATTGGGG AAGACTTGAA TGCTCTTTCA 
GATTCTGCTT GCTCTGTGTC TGTGCTTGAT CCAAGTGAAC CCCGAACACC TGTTGGGAGT 
AGGAAAGCCA GCCGTAACAT ATTTTTAGAG GATGATTTGG CCTGGCAAAG TTTGATTCAT 
CCAGATTCCT CAAATACTCC TCTTTCAACA AGACTTGTAT CTGTGCAAGA GGATGCTGGG 
AAATCTCCTG CTCGAAATAG ATCAGCCAGC ATTACTAACC TGTCACTGGA TAGATCTGGT 
TCTCCTATGG TACCTTCATA TGAGACATCT GTCAGTCCCC AGGCTAACCG AACATATGTT 
AGGACAGAGA CCACTGAGGA TGAACGCAAA ATTCTTCTGG ACAGTGTGCA GTTAAAAGAC 
CTGTGGAAAA AAATCTGCCA TCACAGCAGT GGAATGGAGT TTCAGGATCA CCGCTACTGG 
TTGAGAACGC ATCCCAACTG CATTGTAGGA AAGGAATTAG TCAACTGGCT AATCCGAAAT 
GGGCATATTG CCACAAGGGC ACAAGCTATA GCAATTGGAC AAGCAATGGT TGATGGACGT 
TGGCTGGATT GTGTTAGTCA TCACGACCAG CTTTTCAGAG ATGAGTATGC GCTGTATAGA 
CCACTGCAGA GTACAGAATT TTCTGAGACG CCTTCTCCCG ACAGTGACTC AGTGAACTCC 
GTGGAAGGAC ACTCTGAGCC ATCCTGGTTT AAAGACATAA AGTTTGATGA CAGTGACACA 
GAACAGATAG CTGAAGAAGG TGACGATAAT TTGGCTAAGT ATTTGATTTC TGACACTGGA 
GGACAACAGC TCTCAATAAG TGACGCTTTC ATCAAAGAAT CCTTATTTAA TCGCCGAGTA 
GAGGAAAAAT CCAAAGAGCT GCCTTTCACA CCTTTGGGCT GGCATCATAA CAACCTGGAG 
CTCCTGAGGG AGGAGAATGG GGAGAAACAA GCCATGGAGA GGTTGCTTTC AGCTAATCAT 
AACCACATGA TGGCACTACT CCAGCAGTTG CTCCATAGTG ACTCACTGTC ATCATCTTGG 
AGGGACATCA TCGTGTCATT GGTCTGCCAG GTTGTTCAGA CAGTCCGACC TGATGTCAAG 
AACCAGGATG ATGACATGGA TATCCGTCAG TTTGTCCACA TCAAAAAAAT CCCAGGTGGA 
AAGAAGTTTG ATTCTGTGGT TGTCAATGGC TTTGTTTGTA CCAAGAACAT TGCACATAAA 
AAGATGAGTT CTTGTATTAA AAACCCCAAA ATTCTTCTGT TGAAGTGTTC CATTGAGTAT 
CTCTACAGAG AAGAAACTAA GTTTACTTGC ATTGATCCTA TTGTGCTTCA GGAAAGGGAA 
TTCTTGAAGA ATTATGTCCA GCGAATAGTT GATGTTCGAC CCACCTTGGT TCTTGTTGAG 
AAAACAGTGT CTCGGATTGC CCAGGACATG TTATTGGAAC ATGGCATTAC TTTGGTCATT 
AATGTAAAGT CACAAGTTTT GGAACGAATC AGTCGAATGA CCCAAGGTGA TTTAGTGATG 
TCAATGGACC AGCTGCTTAC GAAACCACAC CTGGGCACTT GTCACAAATT TTATATGCAG 
ATATTTCAGT TGCCTAATGA ACAAACCAAG ACACTGATGT TTTTTGAAGG TTGTCCACAG 
CACCTAGGCT GTACAATCAA GCTAAGAGGA GGCTCTGATT ATGAGCTGGC TCGAGTTAAG 
GAGATCCTAA TATTTATGAT CTGTGTTGCT TATCATTCTC AACTAGAAAT ATCCTTTCTC 
ATGGATGAAT TTGCTATGCC TCCCACATTA ATGCAAAACC CTTCATTCCA TTCCCTGATT 
GAGGGACGAG GGCATGAGGG GGCTGTCCAA GAGCAGTACG GTGGAGGTTC CATCCCCTGG 
GATCCTGACA TCCCTCCTGA GTCTCTGCCC TGTGATGATA GCAGTTTGCT GGAATTGAGG 
ATTGTGTTTG AGAAGGGTGA GCAGGAAAAT AAAAATCTTC CGCAGGCTGT TGCCTCTGTG 
AAGCATCAAG AACATAGCAC AACAGCTTGC CCGGCGGGTC TCCCTTGTGC TTTCTTTGCA 
CCTGTACCGG AATCATTGTT GCCACTCCCT GTGGATGACC AACAAGATGC TTTAGGCAGC 
GAGCAGCCAG AGACTTTGCA GCAAACAGTT GTGCTGCAGG ATCCCAAAAG CCAGATAAGA 
GCCTTTAGAG ACCCTCTACA GGATGACACT GGATTATATG TTACTGAGGA AGTCACCTCC 
TCTGAAGATA AACGAAAGAC TTATTCTTTG GCCTTTAAGC AGGAATTAAA AGATGTGATC 
CTCTGTATCT CCCCAGTAAT CACATTCCGA GAACCCTTTC TTTTAACTGA AAAGGGGATG 
AGATGCTCTA CCCGAGATTA TTTTGCAGAG CAGGTTTACT GGTCTCCTCT CCTCAATAAA 
GAATTCAAAG AAATGGAGAA CAGGAGGAAG AAACAGCTGC TCAGGGATCT CTCTGGACTT 
CAGGGCATGA ATGGAAGTAT TCAGGCCAAG TCTATTCAAG TCTTACCCTC ACATGAGCTA 
GTGAGCACTA GAATTGCTGA GCATCTGGGC GATAGCCAGA GCTTGGGTAG AATGCTGGCC 
GATTATCGAG CCAGAGGAGG AAGAATTCAG CCCAAAAATT CAGACCCTTT TGCTCATTCA 
AAGGATGCAT CAAGTACTTC AAGTGGCCAA TCAGGAAGCA AAAATGAGGG TGATGAAGAG 
AGAGGGCTTA TTCTGAGTGA TGCTGTGTGG TCAACAAAGG TGGACTGTCT GAATCCCATT 
AATCACCAGA GACTTTGTGT GCTCTTCAGC AGCTCTTCTG CCCAGTCCAG CAATGCTCCT 
AGTGCCTGTG TCAGTCCTTG GATTGTAACA ATGGAATTTT ATGGAAAGAA TGATCTTACA 
TTAGGAATAT TTTTAGAGAG ATACTGTTTC AGGCCTTCTT ATCAGTGTCC AAGCATGTTC 
TGTGATACCC CCATGGTACA TCATATTCGG CGCTTTGTTC ATGGCCAAGG CTGTGTGCAG 
ATAATCCTGA AGGAGTTGGA TTCTCCAGTA CCTGGATATC AGCATACAAT TCTTACATAT 
TCCTGGTGTA GAATCTGCAA ACAGGTAACA CCAGTTGTTG CTCTTTCCAA TGAGTCCTGG 
TCTATGTCAT TTGCAAAATA CCTTGAACTT AGGTTTTATG GGCACCAGTA TACTCGCAGA 
GCCAACGCTG AGCCCTGTGG TCACTCCATC CATCATGATT ATCACCAGTA TTTCTCCTAT 
AACCAGATGG TGGCGTCTTT CAGTTATTCT CCCATTCGGC TTCTTGAAGT ATGTGTTCCA 
CTCCCCAAAA TATTCATTAA GCGTCAGGCC CCATTAAAAG TGTCCCTTCT TCAGGATCTG 
AAGGACTTCT TTCAAAAAGT TTCACAGGTA TATGTTGCCA TTGATGAAAG ACTTGCATCT 
TTGAAAACTG ATACATTTAG TAAAACAAGA GAGGAAAAAA TGGAAGATAT TTTTGCACAG 
AAAGAGATGG AAGAAGGTGA GTTCAAGAAC TGGATTGAGA AGATGCAAGC AAGGCTCATG 
TCTTCCTCTG TAGATACCCC TCAGCAACTG CAGTCGGTCT TTGAGTCACT CATTGCCAAG 
AAACAAAGTC TCTGTGAAGT GCTGCAAGCT TGGAATAACA GGTTGCAGGA CCTTTTCCAA 
CAGGAAAAGG GTAGAAAGAG ACCTTCAGTT CCTCCAAGTC CTGGAAGACT GAGACAAGGG 
GAAGAAAGCA AGATAAGTGC GATGGATGCA TCTCCACGGA ATATTTCTCC AGGACTTCAG 
AATGGAGAAA AAGAGGATCG CTTCTTAACA ACTTTGTCCA GCCAGAGCTC CACCAGTTCT 
ACTCATCTCC AATTGCCTAC GCCACCTGAA GTCATGTCTG AACAGTCAGT GGGAGGGCCC 
CCTGAGCTAG ATACAGCCAG CAGTTCCGAA GATGTGTTTG ATGGGCATTT GCTGGGATCC 
ACAGACAGCC AAGTGAAGGA AAAGTCAACC ATGAAAGCCA TCTTTGCAAA TTTGCTTCCA 
GGAAATAGCT ATAATCCTAT TCCATTTCCT TTTGATCCAG ATAAACACTA CTTAATGTAT 
GAACATGAAC GAGTGCCCAT TGCAGTCTGC GAGAAGGAAC CCAGCTCCAT CATTGCTTTT 
GCTCTCAGTT GTAAAGAATA CCGAAATGCC TTAGAGGAAT TGTCTAAAGC GACTCAGTGG 
AACAGTGCCG AAGAAGGGCT TCCAACAAAT AGTACTTCAG ATAGCAGACC AAAGAGTAGC 
AGCCCTATCA GATTACCTGA AATGAGTGGA GGACAGACAA ATCGTACAAC AGAAACAGAA 
CCACAACCAA CCAAAAAGGC TTCTGGAATG TTGTCCTTCT TCAGAGGGAC AGCAGGGAAA 
AGCCCCGATC TCTCTTCCCA GAAGAGAGAG ACCTTACGTG GAGCAGATAG TGCTTACTAC 
CAGGTTGGGC AGACGGGCAA GGAGGGGACC GAGAATCAAG GCGTTGAGCC TCAAGATGAA 
GTAGATGGAG GAGATACACA AAAGAAGCAA CTCATAAATC CTCATGTGGA ACTTCAATTT 
TCAGATGCTA ATGCCAAGTT TTACTGTCGG CTCTACTATG CGGGAGAGTT TCATAAGATG 
CGTGAAGTGA TTCTGGACAG CAGTGAAGAA GATTTCATTC GTTCCCTCTC CCACTCATCA 
CCCTGGCAGG CCCGGGGAGG CAAATCAGGA GCTGCCTTCT ATGCAACTGA GGATGATAGA 
TTTATTTTGA AGCAAATGCC TCGTCTGGAA GTCCAGTCCT TCCTCGACTT TGCACCACAT 
TACTTCAATT ATATTACAAA TGCTGTTCAA CAAAAGAGGC CCACGGCGTT GGCCAAAATT 
CTTGGAGTTT ACAGAATTGG TTATAAGAAC TCTCAGAACA ACACTGAGAA GAAGTTAGAT 
CTCCTTGTCA TGGAAAATCT TTTCTACGGG AGAAAGATGG CACAGGTTTT TGATTTGAAG 
GGCTCTCTTA GGAATCGGAA TGTAAAAACT GACACTGGAA AAGAGAGTTG TGATGTGGTC 
CTGCTAGATG AAAATCTCCT AAAGATGGTT CGAGACAACC CTCTATATAT TCGTTCTCAT 
TCCAAAGCTG TGCTGAGAAC CTCGATCCAT AGTGACTCCC ATTTCCTTTC TAGCCACCTC 
ATTATAGATT ATTCTTTGCT GGTTGGGCGA GATGATACTA GCAATGAGCT AGTAGTTGGA 
ATTATAGATT ATATTCGAAC ATTTACATGG GACAAAAAGC TTGAGATGGT TGTGAAATCA 
ACAGGAATTT TAGGTGGACA AGGTAAAATG CCAACAGTGG TGTCTCCGGA GTTGTACAGG 
ACTAGGTTTT GTGAGGCAAT GGACAAGTAT TTCCTAATGG TACCAGACCA CTGGACAGGC 
TTGGGTCTGA ATTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PIKFYVE ( XM_017003571.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016859060.1 Homo sapiens phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X16, mRNA.

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CloneID	OHu89146
Clone ID Related Accession (Same CDS sequence)	XM_017003571.1
Accession Version	XM_017003571.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5688bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	1-phosphatidylinositol 3-phosphate 5-kinase isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG ATGATAAGAC GTCCCCAACA CTGGACTCTG CTAATGATTT GCCTCGATCT 
CCTACTAGTC CTTCTCATCT CACACACTTT AAACCTTTGA CTCCTGATCA AGATGAGCCC 
CCTTTTAAAT CAGCTTATAG TTCTTTTGTA AATCTCTTTC GTTTTAACAA AGGAGACCTC 
CGAGCTTGCA CATATTGTAG AAAAATAGCC TTAAGTTATG CTCATTCCAC AGACAGTAAT 
TCTATTGGGG AAGACTTGAA TGCTCTTTCA GATTCTGCTT GCTCTGTGTC TGTGCTTGAT 
CCAAGTGAAC CCCGAACACC TGTTGGGAGT AGGAAAGCCA GCCGTAACAT ATTTTTAGAG 
GATGATTTGG CCTGGCAAAG TTTGATTCAT CCAGATTCCT CAAATACTCC TCTTTCAACA 
AGACTTGTAT CTGTGCAAGA GGATGCTGGG AAATCTCCTG CTCGAAATAG ATCAGCCAGC 
ATTACTAACC TGTCACTGGA TAGATCTGGT TCTCCTATGG TACCTTCATA TGAGACATCT 
GTCAGTCCCC AGGCTAACCG AACATATGTT AGGACAGAGA CCACTGAGGA TGAACGCAAA 
ATTCTTCTGG ACAGTGTGCA GTTAAAAGAC CTGTGGAAAA AAATCTGCCA TCACAGCAGT 
GGAATGGAGT TTCAGGATCA CCGCTACTGG TTGAGAACGC ATCCCAACTG CATTGTAGGA 
AAGGAATTAG TCAACTGGCT AATCCGAAAT GGGCATATTG CCACAAGGGC ACAAGCTATA 
GCAATTGGAC AAGCAATGGT TGATGGACGT TGGCTGGATT GTGTTAGTCA TCACGACCAG 
CTTTTCAGAG ATGAGTATGC GCTGTATAGA CCACTGCAGA GTACAGAATT TTCTGAGACG 
CCTTCTCCCG ACAGTGACTC AGTGAACTCC GTGGAAGGAC ACTCTGAGCC ATCCTGGTTT 
AAAGACATAA AGTTTGATGA CAGTGACACA GAACAGATAG CTGAAGAAGG TGACGATAAT 
TTGGCTAAGT ATTTGATTTC TGACACTGGA GGACAACAGC TCTCAATAAG TGACGCTTTC 
ATCAAAGAAT CCTTATTTAA TCGCCGAGTA GAGGAAAAAT CCAAAGAGCT GCCTTTCACA 
CCTTTGGGCT GGCATCATAA CAACCTGGAG CTCCTGAGGG AGGAGAATGG GGAGAAACAA 
GCCATGGAGA GGTTGCTTTC AGCTAATCAT AACCACATGA TGGCACTACT CCAGCAGTTG 
CTCCATAGTG ACTCACTGTC ATCATCTTGG AGGGACATCA TCGTGTCATT GGTCTGCCAG 
GTTGTTCAGA CAGTCCGACC TGATGTCAAG AACCAGGATG ATGACATGGA TATCCGTCAG 
TTTGTCCACA TCAAAAAAAT CCCAGGTGGA AAGAAGTTTG ATTCTGTGGT TGTCAATGGC 
TTTGTTTGTA CCAAGAACAT TGCACATAAA AAGATGAGTT CTTGTATTAA AAACCCCAAA 
ATTCTTCTGT TGAAGTGTTC CATTGAGTAT CTCTACAGAG AAGAAACTAA GTTTACTTGC 
ATTGATCCTA TTGTGCTTCA GGAAAGGGAA TTCTTGAAGA ATTATGTCCA GCGAATAGTT 
GATGTTCGAC CCACCTTGGT TCTTGTTGAG AAAACAGTGT CTCGGATTGC CCAGGACATG 
TTATTGGAAC ATGGCATTAC TTTGGTCATT AATGTAAAGT CACAAGTTTT GGAACGAATC 
AGTCGAATGA CCCAAGGTGA TTTAGTGATG TCAATGGACC AGCTGCTTAC GAAACCACAC 
CTGGGCACTT GTCACAAATT TTATATGCAG ATATTTCAGT TGCCTAATGA ACAAACCAAG 
ACACTGATGT TTTTTGAAGG TTGTCCACAG CACCTAGGCT GTACAATCAA GCTAAGAGGA 
GGCTCTGATT ATGAGCTGGC TCGAGTTAAG GAGATCCTAA TATTTATGAT CTGTGTTGCT 
TATCATTCTC AACTAGAAAT ATCCTTTCTC ATGGATGAAT TTGCTATGCC TCCCACATTA 
ATGCAAAACC CTTCATTCCA TTCCCTGATT GAGGGACGAG GGCATGAGGG GGCTGTCCAA 
GAGCAGTACG GTGGAGGTTC CATCCCCTGG GATCCTGACA TCCCTCCTGA GTCTCTGCCC 
TGTGATGATA GCAGTTTGCT GGAATTGAGG ATTGTGTTTG AGAAGGGTGA GCAGGAAAAT 
AAAAATCTTC CGCAGGCTGT TGCCTCTGTG AAGCATCAAG AACATAGCAC AACAGCTTGC 
CCGGCGGGTC TCCCTTGTGC TTTCTTTGCA CCTGTACCGG AATCATTGTT GCCACTCCCT 
GTGGATGACC AACAAGATGC TTTAGGCAGC GAGCAGCCAG AGACTTTGCA GCAAACAGTT 
GTGCTGCAGG ATCCCAAAAG CCAGATAAGA GCCTTTAGAG ACCCTCTACA GGATGACACT 
GGATTATATG TTACTGAGGA AGTCACCTCC TCTGAAGATA AACGAAAGAC TTATTCTTTG 
GCCTTTAAGC AGGAATTAAA AGATGTGATC CTCTGTATCT CCCCAGTAAT CACATTCCGA 
GAACCCTTTC TTTTAACTGA AAAGGGGATG AGATGCTCTA CCCGAGATTA TTTTGCAGAG 
CAGGTTTACT GGTCTCCTCT CCTCAATAAA GAATTCAAAG AAATGGAGAA CAGGAGGAAG 
AAACAGCTGC TCAGGGATCT CTCTGGACTT CAGGGCATGA ATGGAAGTAT TCAGGCCAAG 
TCTATTCAAG TCTTACCCTC ACATGAGCTA GTGAGCACTA GAATTGCTGA GCATCTGGGC 
GATAGCCAGA GCTTGGGTAG AATGCTGGCC GATTATCGAG CCAGAGGAGG AAGAATTCAG 
CCCAAAAATT CAGACCCTTT TGCTCATTCA AAGGATGCAT CAAGTACTTC AAGTGGCCAA 
TCAGGAAGCA AAAATGAGGG TGATGAAGAG AGAGGGCTTA TTCTGAGTGA TGCTGTGTGG 
TCAACAAAGG TGGACTGTCT GAATCCCATT AATCACCAGA GACTTTGTGT GCTCTTCAGC 
AGCTCTTCTG CCCAGTCCAG CAATGCTCCT AGTGCCTGTG TCAGTCCTTG GATTGTAACA 
ATGGAATTTT ATGGAAAGAA TGATCTTACA TTAGGAATAT TTTTAGAGAG ATACTGTTTC 
AGGCCTTCTT ATCAGTGTCC AAGCATGTTC TGTGATACCC CCATGGTACA TCATATTCGG 
CGCTTTGTTC ATGGCCAAGG CTGTGTGCAG ATAATCCTGA AGGAGTTGGA TTCTCCAGTA 
CCTGGATATC AGCATACAAT TCTTACATAT TCCTGGTGTA GAATCTGCAA ACAGGTAACA 
CCAGTTGTTG CTCTTTCCAA TGAGTCCTGG TCTATGTCAT TTGCAAAATA CCTTGAACTT 
AGGTTTTATG GGCACCAGTA TACTCGCAGA GCCAACGCTG AGCCCTGTGG TCACTCCATC 
CATCATGATT ATCACCAGTA TTTCTCCTAT AACCAGATGG TGGCGTCTTT CAGTTATTCT 
CCCATTCGGC TTCTTGAAGT ATGTGTTCCA CTCCCCAAAA TATTCATTAA GCGTCAGGCC 
CCATTAAAAG TGTCCCTTCT TCAGGATCTG AAGGACTTCT TTCAAAAAGT TTCACAGGTA 
TATGTTGCCA TTGATGAAAG ACTTGCATCT TTGAAAACTG ATACATTTAG TAAAACAAGA 
GAGGAAAAAA TGGAAGATAT TTTTGCACAG AAAGAGATGG AAGAAGGTGA GTTCAAGAAC 
TGGATTGAGA AGATGCAAGC AAGGCTCATG TCTTCCTCTG TAGATACCCC TCAGCAACTG 
CAGTCGGTCT TTGAGTCACT CATTGCCAAG AAACAAAGTC TCTGTGAAGT GCTGCAAGCT 
TGGAATAACA GGTTGCAGGA CCTTTTCCAA CAGGAAAAGG GTAGAAAGAG ACCTTCAGTT 
CCTCCAAGTC CTGGAAGACT GAGACAAGGG GAAGAAAGCA AGATAAGTGC GATGGATGCA 
TCTCCACGGA ATATTTCTCC AGGACTTCAG AATGGAGAAA AAGAGGATCG CTTCTTAACA 
ACTTTGTCCA GCCAGAGCTC CACCAGTTCT ACTCATCTCC AATTGCCTAC GCCACCTGAA 
GTCATGTCTG AACAGTCAGT GGGAGGGCCC CCTGAGCTAG ATACAGCCAG CAGTTCCGAA 
GATGTGTTTG ATGGGCATTT GCTGGGATCC ACAGACAGCC AAGTGAAGGA AAAGTCAACC 
ATGAAAGCCA TCTTTGCAAA TTTGCTTCCA GGAAATAGCT ATAATCCTAT TCCATTTCCT 
TTTGATCCAG ATAAACACTA CTTAATGTAT GAACATGAAC GAGTGCCCAT TGCAGTCTGC 
GAGAAGGAAC CCAGCTCCAT CATTGCTTTT GCTCTCAGTT GTAAAGAATA CCGAAATGCC 
TTAGAGGAAT TGTCTAAAGC GACTCAGTGG AACAGTGCCG AAGAAGGGCT TCCAACAAAT 
AGTACTTCAG ATAGCAGACC AAAGAGTAGC AGCCCTATCA GATTACCTGA AATGAGTGGA 
GGACAGACAA ATCGTACAAC AGAAACAGAA CCACAACCAA CCAAAAAGGC TTCTGGAATG 
TTGTCCTTCT TCAGAGGGAC AGCAGGGAAA AGCCCCGATC TCTCTTCCCA GAAGAGAGAG 
ACCTTACGTG GAGCAGATAG TGCTTACTAC CAGGTTGGGC AGACGGGCAA GGAGGGGACC 
GAGAATCAAG GCGTTGAGCC TCAAGATGAA GTAGATGGAG GAGATACACA AAAGAAGCAA 
CTCATAAATC CTCATGTGGA ACTTCAATTT TCAGATGCTA ATGCCAAGTT TTACTGTCGG 
CTCTACTATG CGGGAGAGTT TCATAAGATG CGTGAAGTGA TTCTGGACAG CAGTGAAGAA 
GATTTCATTC GTTCCCTCTC CCACTCATCA CCCTGGCAGG CCCGGGGAGG CAAATCAGGA 
GCTGCCTTCT ATGCAACTGA GGATGATAGA TTTATTTTGA AGCAAATGCC TCGTCTGGAA 
GTCCAGTCCT TCCTCGACTT TGCACCACAT TACTTCAATT ATATTACAAA TGCTGTTCAA 
CAAAAGAGGC CCACGGCGTT GGCCAAAATT CTTGGAGTTT ACAGAATTGG TTATAAGAAC 
TCTCAGAACA ACACTGAGAA GAAGTTAGAT CTCCTTGTCA TGGAAAATCT TTTCTACGGG 
AGAAAGATGG CACAGGTTTT TGATTTGAAG GGCTCTCTTA GGAATCGGAA TGTAAAAACT 
GACACTGGAA AAGAGAGTTG TGATGTGGTC CTGCTAGATG AAAATCTCCT AAAGATGGTT 
CGAGACAACC CTCTATATAT TCGTTCTCAT TCCAAAGCTG TGCTGAGAAC CTCGATCCAT 
AGTGACTCCC ATTTCCTTTC TAGCCACCTC ATTATAGATT ATTCTTTGCT GGTTGGGCGA 
GATGATACTA GCAATGAGCT AGTAGTTGGA ATTATAGATT ATATTCGAAC ATTTACATGG 
GACAAAAAGC TTGAGATGGT TGTGAAATCA ACAGGAATTT TAGGTGGACA AGGTAAAATG 
CCAACAGTGG TGTCTCCGGA GTTGTACAGG ACTAGGTTTT GTGAGGCAAT GGACAAGTAT 
TTCCTAATGG TACCAGACCA CTGGACAGGC TTGGGTCTGA ATTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016859060.1
CDS	131..5818
Translation	MATDDKTSPTLDSANDLPRSPTSPSHLTHFKPLTPDQDEPPFKSAYSSFVNLFRFNKGDLRACTYCRKIALSYAHSTDSNSIGEDLNALSDSACSVSVLDPSEPRTPVGSRKASRNIFLEDDLAWQSLIHPDSSNTPLSTRLVSVQEDAGKSPARNRSASITNLSLDRSGSPMVPSYETSVSPQANRTYVRTETTEDERKILLDSVQLKDLWKKICHHSSGMEFQDHRYWLRTHPNCIVGKELVNWLIRNGHIATRAQAIAIGQAMVDGRWLDCVSHHDQLFRDEYALYRPLQSTEFSETPSPDSDSVNSVEGHSEPSWFKDIKFDDSDTEQIAEEGDDNLAKYLISDTGGQQLSISDAFIKESLFNRRVEEKSKELPFTPLGWHHNNLELLREENGEKQAMERLLSANHNHMMALLQQLLHSDSLSSSWRDIIVSLVCQVVQTVRPDVKNQDDDMDIRQFVHIKKIPGGKKFDSVVVNGFVCTKNIAHKKMSSCIKNPKILLLKCSIEYLYREETKFTCIDPIVLQEREFLKNYVQRIVDVRPTLVLVEKTVSRIAQDMLLEHGITLVINVKSQVLERISRMTQGDLVMSMDQLLTKPHLGTCHKFYMQIFQLPNEQTKTLMFFEGCPQHLGCTIKLRGGSDYELARVKEILIFMICVAYHSQLEISFLMDEFAMPPTLMQNPSFHSLIEGRGHEGAVQEQYGGGSIPWDPDIPPESLPCDDSSLLELRIVFEKGEQENKNLPQAVASVKHQEHSTTACPAGLPCAFFAPVPESLLPLPVDDQQDALGSEQPETLQQTVVLQDPKSQIRAFRDPLQDDTGLYVTEEVTSSEDKRKTYSLAFKQELKDVILCISPVITFREPFLLTEKGMRCSTRDYFAEQVYWSPLLNKEFKEMENRRKKQLLRDLSGLQGMNGSIQAKSIQVLPSHELVSTRIAEHLGDSQSLGRMLADYRARGGRIQPKNSDPFAHSKDASSTSSGQSGSKNEGDEERGLILSDAVWSTKVDCLNPINHQRLCVLFSSSSAQSSNAPSACVSPWIVTMEFYGKNDLTLGIFLERYCFRPSYQCPSMFCDTPMVHHIRRFVHGQGCVQIILKELDSPVPGYQHTILTYSWCRICKQVTPVVALSNESWSMSFAKYLELRFYGHQYTRRANAEPCGHSIHHDYHQYFSYNQMVASFSYSPIRLLEVCVPLPKIFIKRQAPLKVSLLQDLKDFFQKVSQVYVAIDERLASLKTDTFSKTREEKMEDIFAQKEMEEGEFKNWIEKMQARLMSSSVDTPQQLQSVFESLIAKKQSLCEVLQAWNNRLQDLFQQEKGRKRPSVPPSPGRLRQGEESKISAMDASPRNISPGLQNGEKEDRFLTTLSSQSSTSSTHLQLPTPPEVMSEQSVGGPPELDTASSSEDVFDGHLLGSTDSQVKEKSTMKAIFANLLPGNSYNPIPFPFDPDKHYLMYEHERVPIAVCEKEPSSIIAFALSCKEYRNALEELSKATQWNSAEEGLPTNSTSDSRPKSSSPIRLPEMSGGQTNRTTETEPQPTKKASGMLSFFRGTAGKSPDLSSQKRETLRGADSAYYQVGQTGKEGTENQGVEPQDEVDGGDTQKKQLINPHVELQFSDANAKFYCRLYYAGEFHKMREVILDSSEEDFIRSLSHSSPWQARGGKSGAAFYATEDDRFILKQMPRLEVQSFLDFAPHYFNYITNAVQQKRPTALAKILGVYRIGYKNSQNNTEKKLDLLVMENLFYGRKMAQVFDLKGSLRNRNVKTDTGKESCDVVLLDENLLKMVRDNPLYIRSHSKAVLRTSIHSDSHFLSSHLIIDYSLLVGRDDTSNELVVGIIDYIRTFTWDKKLEMVVKSTGILGGQGKMPTVVSPELYRTRFCEAMDKYFLMVPDHWTGLGLNC

Target ORF information:

RefSeq Version	XM_017003571.1
Organism	Homo sapiens(human)
Definition	Homo sapiens phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017003571.1

ORF Insert Sequence:

ATGGCCACAG ATGATAAGAC GTCCCCAACA CTGGACTCTG CTAATGATTT GCCTCGATCT 
CCTACTAGTC CTTCTCATCT CACACACTTT AAACCTTTGA CTCCTGATCA AGATGAGCCC 
CCTTTTAAAT CAGCTTATAG TTCTTTTGTA AATCTCTTTC GTTTTAACAA AGGAGACCTC 
CGAGCTTGCA CATATTGTAG AAAAATAGCC TTAAGTTATG CTCATTCCAC AGACAGTAAT 
TCTATTGGGG AAGACTTGAA TGCTCTTTCA GATTCTGCTT GCTCTGTGTC TGTGCTTGAT 
CCAAGTGAAC CCCGAACACC TGTTGGGAGT AGGAAAGCCA GCCGTAACAT ATTTTTAGAG 
GATGATTTGG CCTGGCAAAG TTTGATTCAT CCAGATTCCT CAAATACTCC TCTTTCAACA 
AGACTTGTAT CTGTGCAAGA GGATGCTGGG AAATCTCCTG CTCGAAATAG ATCAGCCAGC 
ATTACTAACC TGTCACTGGA TAGATCTGGT TCTCCTATGG TACCTTCATA TGAGACATCT 
GTCAGTCCCC AGGCTAACCG AACATATGTT AGGACAGAGA CCACTGAGGA TGAACGCAAA 
ATTCTTCTGG ACAGTGTGCA GTTAAAAGAC CTGTGGAAAA AAATCTGCCA TCACAGCAGT 
GGAATGGAGT TTCAGGATCA CCGCTACTGG TTGAGAACGC ATCCCAACTG CATTGTAGGA 
AAGGAATTAG TCAACTGGCT AATCCGAAAT GGGCATATTG CCACAAGGGC ACAAGCTATA 
GCAATTGGAC AAGCAATGGT TGATGGACGT TGGCTGGATT GTGTTAGTCA TCACGACCAG 
CTTTTCAGAG ATGAGTATGC GCTGTATAGA CCACTGCAGA GTACAGAATT TTCTGAGACG 
CCTTCTCCCG ACAGTGACTC AGTGAACTCC GTGGAAGGAC ACTCTGAGCC ATCCTGGTTT 
AAAGACATAA AGTTTGATGA CAGTGACACA GAACAGATAG CTGAAGAAGG TGACGATAAT 
TTGGCTAAGT ATTTGATTTC TGACACTGGA GGACAACAGC TCTCAATAAG TGACGCTTTC 
ATCAAAGAAT CCTTATTTAA TCGCCGAGTA GAGGAAAAAT CCAAAGAGCT GCCTTTCACA 
CCTTTGGGCT GGCATCATAA CAACCTGGAG CTCCTGAGGG AGGAGAATGG GGAGAAACAA 
GCCATGGAGA GGTTGCTTTC AGCTAATCAT AACCACATGA TGGCACTACT CCAGCAGTTG 
CTCCATAGTG ACTCACTGTC ATCATCTTGG AGGGACATCA TCGTGTCATT GGTCTGCCAG 
GTTGTTCAGA CAGTCCGACC TGATGTCAAG AACCAGGATG ATGACATGGA TATCCGTCAG 
TTTGTCCACA TCAAAAAAAT CCCAGGTGGA AAGAAGTTTG ATTCTGTGGT TGTCAATGGC 
TTTGTTTGTA CCAAGAACAT TGCACATAAA AAGATGAGTT CTTGTATTAA AAACCCCAAA 
ATTCTTCTGT TGAAGTGTTC CATTGAGTAT CTCTACAGAG AAGAAACTAA GTTTACTTGC 
ATTGATCCTA TTGTGCTTCA GGAAAGGGAA TTCTTGAAGA ATTATGTCCA GCGAATAGTT 
GATGTTCGAC CCACCTTGGT TCTTGTTGAG AAAACAGTGT CTCGGATTGC CCAGGACATG 
TTATTGGAAC ATGGCATTAC TTTGGTCATT AATGTAAAGT CACAAGTTTT GGAACGAATC 
AGTCGAATGA CCCAAGGTGA TTTAGTGATG TCAATGGACC AGCTGCTTAC GAAACCACAC 
CTGGGCACTT GTCACAAATT TTATATGCAG ATATTTCAGT TGCCTAATGA ACAAACCAAG 
ACACTGATGT TTTTTGAAGG TTGTCCACAG CACCTAGGCT GTACAATCAA GCTAAGAGGA 
GGCTCTGATT ATGAGCTGGC TCGAGTTAAG GAGATCCTAA TATTTATGAT CTGTGTTGCT 
TATCATTCTC AACTAGAAAT ATCCTTTCTC ATGGATGAAT TTGCTATGCC TCCCACATTA 
ATGCAAAACC CTTCATTCCA TTCCCTGATT GAGGGACGAG GGCATGAGGG GGCTGTCCAA 
GAGCAGTACG GTGGAGGTTC CATCCCCTGG GATCCTGACA TCCCTCCTGA GTCTCTGCCC 
TGTGATGATA GCAGTTTGCT GGAATTGAGG ATTGTGTTTG AGAAGGGTGA GCAGGAAAAT 
AAAAATCTTC CGCAGGCTGT TGCCTCTGTG AAGCATCAAG AACATAGCAC AACAGCTTGC 
CCGGCGGGTC TCCCTTGTGC TTTCTTTGCA CCTGTACCGG AATCATTGTT GCCACTCCCT 
GTGGATGACC AACAAGATGC TTTAGGCAGC GAGCAGCCAG AGACTTTGCA GCAAACAGTT 
GTGCTGCAGG ATCCCAAAAG CCAGATAAGA GCCTTTAGAG ACCCTCTACA GGATGACACT 
GGATTATATG TTACTGAGGA AGTCACCTCC TCTGAAGATA AACGAAAGAC TTATTCTTTG 
GCCTTTAAGC AGGAATTAAA AGATGTGATC CTCTGTATCT CCCCAGTAAT CACATTCCGA 
GAACCCTTTC TTTTAACTGA AAAGGGGATG AGATGCTCTA CCCGAGATTA TTTTGCAGAG 
CAGGTTTACT GGTCTCCTCT CCTCAATAAA GAATTCAAAG AAATGGAGAA CAGGAGGAAG 
AAACAGCTGC TCAGGGATCT CTCTGGACTT CAGGGCATGA ATGGAAGTAT TCAGGCCAAG 
TCTATTCAAG TCTTACCCTC ACATGAGCTA GTGAGCACTA GAATTGCTGA GCATCTGGGC 
GATAGCCAGA GCTTGGGTAG AATGCTGGCC GATTATCGAG CCAGAGGAGG AAGAATTCAG 
CCCAAAAATT CAGACCCTTT TGCTCATTCA AAGGATGCAT CAAGTACTTC AAGTGGCCAA 
TCAGGAAGCA AAAATGAGGG TGATGAAGAG AGAGGGCTTA TTCTGAGTGA TGCTGTGTGG 
TCAACAAAGG TGGACTGTCT GAATCCCATT AATCACCAGA GACTTTGTGT GCTCTTCAGC 
AGCTCTTCTG CCCAGTCCAG CAATGCTCCT AGTGCCTGTG TCAGTCCTTG GATTGTAACA 
ATGGAATTTT ATGGAAAGAA TGATCTTACA TTAGGAATAT TTTTAGAGAG ATACTGTTTC 
AGGCCTTCTT ATCAGTGTCC AAGCATGTTC TGTGATACCC CCATGGTACA TCATATTCGG 
CGCTTTGTTC ATGGCCAAGG CTGTGTGCAG ATAATCCTGA AGGAGTTGGA TTCTCCAGTA 
CCTGGATATC AGCATACAAT TCTTACATAT TCCTGGTGTA GAATCTGCAA ACAGGTAACA 
CCAGTTGTTG CTCTTTCCAA TGAGTCCTGG TCTATGTCAT TTGCAAAATA CCTTGAACTT 
AGGTTTTATG GGCACCAGTA TACTCGCAGA GCCAACGCTG AGCCCTGTGG TCACTCCATC 
CATCATGATT ATCACCAGTA TTTCTCCTAT AACCAGATGG TGGCGTCTTT CAGTTATTCT 
CCCATTCGGC TTCTTGAAGT ATGTGTTCCA CTCCCCAAAA TATTCATTAA GCGTCAGGCC 
CCATTAAAAG TGTCCCTTCT TCAGGATCTG AAGGACTTCT TTCAAAAAGT TTCACAGGTA 
TATGTTGCCA TTGATGAAAG ACTTGCATCT TTGAAAACTG ATACATTTAG TAAAACAAGA 
GAGGAAAAAA TGGAAGATAT TTTTGCACAG AAAGAGATGG AAGAAGGTGA GTTCAAGAAC 
TGGATTGAGA AGATGCAAGC AAGGCTCATG TCTTCCTCTG TAGATACCCC TCAGCAACTG 
CAGTCGGTCT TTGAGTCACT CATTGCCAAG AAACAAAGTC TCTGTGAAGT GCTGCAAGCT 
TGGAATAACA GGTTGCAGGA CCTTTTCCAA CAGGAAAAGG GTAGAAAGAG ACCTTCAGTT 
CCTCCAAGTC CTGGAAGACT GAGACAAGGG GAAGAAAGCA AGATAAGTGC GATGGATGCA 
TCTCCACGGA ATATTTCTCC AGGACTTCAG AATGGAGAAA AAGAGGATCG CTTCTTAACA 
ACTTTGTCCA GCCAGAGCTC CACCAGTTCT ACTCATCTCC AATTGCCTAC GCCACCTGAA 
GTCATGTCTG AACAGTCAGT GGGAGGGCCC CCTGAGCTAG ATACAGCCAG CAGTTCCGAA 
GATGTGTTTG ATGGGCATTT GCTGGGATCC ACAGACAGCC AAGTGAAGGA AAAGTCAACC 
ATGAAAGCCA TCTTTGCAAA TTTGCTTCCA GGAAATAGCT ATAATCCTAT TCCATTTCCT 
TTTGATCCAG ATAAACACTA CTTAATGTAT GAACATGAAC GAGTGCCCAT TGCAGTCTGC 
GAGAAGGAAC CCAGCTCCAT CATTGCTTTT GCTCTCAGTT GTAAAGAATA CCGAAATGCC 
TTAGAGGAAT TGTCTAAAGC GACTCAGTGG AACAGTGCCG AAGAAGGGCT TCCAACAAAT 
AGTACTTCAG ATAGCAGACC AAAGAGTAGC AGCCCTATCA GATTACCTGA AATGAGTGGA 
GGACAGACAA ATCGTACAAC AGAAACAGAA CCACAACCAA CCAAAAAGGC TTCTGGAATG 
TTGTCCTTCT TCAGAGGGAC AGCAGGGAAA AGCCCCGATC TCTCTTCCCA GAAGAGAGAG 
ACCTTACGTG GAGCAGATAG TGCTTACTAC CAGGTTGGGC AGACGGGCAA GGAGGGGACC 
GAGAATCAAG GCGTTGAGCC TCAAGATGAA GTAGATGGAG GAGATACACA AAAGAAGCAA 
CTCATAAATC CTCATGTGGA ACTTCAATTT TCAGATGCTA ATGCCAAGTT TTACTGTCGG 
CTCTACTATG CGGGAGAGTT TCATAAGATG CGTGAAGTGA TTCTGGACAG CAGTGAAGAA 
GATTTCATTC GTTCCCTCTC CCACTCATCA CCCTGGCAGG CCCGGGGAGG CAAATCAGGA 
GCTGCCTTCT ATGCAACTGA GGATGATAGA TTTATTTTGA AGCAAATGCC TCGTCTGGAA 
GTCCAGTCCT TCCTCGACTT TGCACCACAT TACTTCAATT ATATTACAAA TGCTGTTCAA 
CAAAAGAGGC CCACGGCGTT GGCCAAAATT CTTGGAGTTT ACAGAATTGG TTATAAGAAC 
TCTCAGAACA ACACTGAGAA GAAGTTAGAT CTCCTTGTCA TGGAAAATCT TTTCTACGGG 
AGAAAGATGG CACAGGTTTT TGATTTGAAG GGCTCTCTTA GGAATCGGAA TGTAAAAACT 
GACACTGGAA AAGAGAGTTG TGATGTGGTC CTGCTAGATG AAAATCTCCT AAAGATGGTT 
CGAGACAACC CTCTATATAT TCGTTCTCAT TCCAAAGCTG TGCTGAGAAC CTCGATCCAT 
AGTGACTCCC ATTTCCTTTC TAGCCACCTC ATTATAGATT ATTCTTTGCT GGTTGGGCGA 
GATGATACTA GCAATGAGCT AGTAGTTGGA ATTATAGATT ATATTCGAAC ATTTACATGG 
GACAAAAAGC TTGAGATGGT TGTGAAATCA ACAGGAATTT TAGGTGGACA AGGTAAAATG 
CCAACAGTGG TGTCTCCGGA GTTGTACAGG ACTAGGTTTT GTGAGGCAAT GGACAAGTAT 
TTCCTAATGG TACCAGACCA CTGGACAGGC TTGGGTCTGA ATTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PIKFYVE ( XM_011510789.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011509091.1 Homo sapiens phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X14, mRNA.

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CloneID	OHu77671
Clone ID Related Accession (Same CDS sequence)	XM_011510789.2
Accession Version	XM_011510789.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5856bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	1-phosphatidylinositol 3-phosphate 5-kinase isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011510789.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG ATGATAAGAC GTCCCCAACA CTGGACTCTG CTAATGATTT GCCTCGATCT 
CCTACTAGTC CTTCTCATCT CACACACTTT AAACCTTTGA CTCCTGATCA AGATGAGCCC 
CCTTTTAAAT CAGCTTATAG TTCTTTTGTA AATCTCTTTC GTTTTAACAA AGGAGACCTC 
CGAGCTTGCA CATATTGTAG AAAAATAGCC TTAAGTTATG CTCATTCCAC AGACAGTAAT 
TCTATTGGGG AAGACTTGAA TGCTCTTTCA GATTCTGCTT GCTCTGTGTC TGTGCTTGAT 
CCAAGTGAAC CCCGAACACC TGTTGGGAGT AGGAAAGCCA GCCGTAACAT ATTTTTAGAG 
GATGATTTGG CCTGGCAAAG TTTGATTCAT CCAGATTCCT CAAATACTCC TCTTTCAACA 
AGACTTGTAT CTGTGCAAGA GGATGCTGGG AAATCTCCTG CTCGAAATAG ATCAGCCAGC 
ATTACTAACC TGTCACTGGA TAGATCTGGT TCTCCTATGG TACCTTCATA TGAGACATCT 
GTCAGTCCCC AGGCTAACCG AACATATGTT AGGACAGAGA CCACTGAGGA TGAACGCAAA 
ATTCTTCTGG ACAGTGTGCA GTTAAAAGAC CTGTGGAAAA AAATCTGCCA TCACAGCAGT 
GGAATGGAGT TTCAGGATCA CCGCTACTGG TTGAGAACGC ATCCCAACTG CATTGTAGGA 
AAGGAATTAG TCAACTGGCT AATCCGAAAT GGGCATATTG CCACAAGGGC ACAAGCTATA 
GCAATTGGAC AAGCAATGGT TGATGGACGT TGGCTGGATT GTGTTAGTCA TCACGACCAG 
CTTTTCAGAG ATGAGTATGC GCTGTATAGA CCACTGCAGA GTACAGAATT TTCTGAGACG 
CCTTCTCCCG ACAGTGACTC AGTGAACTCC GTGGAAGGAC ACTCTGAGCC ATCCTGGTTT 
AAAGACATAA AGTTTGATGA CAGTGACACA GAACAGATAG CTGAAGAAGG TGACGATAAT 
TTGGCTAATT CTGCCAGTCC TAGCAAGCGC ACATCAGTCA GCAGTTTCCA GTCCACAGTG 
GACAGTGACT CAGCCGCTTC TATCAGCCTG AACGTGGAGC TGGACAACGT GAACTTCCAT 
ATCAAGAAGC CCTCCAAGTA CCCACATGTG CCCCCTCACC CTGCTGACCA AAAAGAGTAT 
TTGATTTCTG ACACTGGAGG ACAACAGCTC TCAATAAGTG ACGCTTTCAT CAAAGAATCC 
TTATTTAATC GCCGAGTAGA GGAAAAATCC AAAGAGCTGC CTTTCACACC TTTGGGCTGG 
CATCATAACA ACCTGGAGCT CCTGAGGGAG GAGAATGGGG AGAAACAAGC CATGGAGAGG 
TTGCTTTCAG CTAATCATAA CCACATGATG GCACTACTCC AGCAGTTGCT CCATAGTGAC 
TCACTGTCAT CATCTTGGAG GGACATCATC GTGTCATTGG TCTGCCAGGT TGTTCAGACA 
GTCCGACCTG ATGTCAAGAA CCAGGATGAT GACATGGATA TCCGTCAGTT TGTCCACATC 
AAAAAAATCC CAGGTGGAAA GAAGTTTGAT TCTGTGGTTG TCAATGGCTT TGTTTGTACC 
AAGAACATTG CACATAAAAA GATGAGTTCT TGTATTAAAA ACCCCAAAAT TCTTCTGTTG 
AAGTGTTCCA TTGAGTATCT CTACAGAGAA GAAACTAAGT TTACTTGCAT TGATCCTATT 
GTGCTTCAGG AAAGGGAATT CTTGAAGAAT TATGTCCAGC GAATAGTTGA TGTTCGACCC 
ACCTTGGTTC TTGTTGAGAA AACAGTGTCT CGGATTGCCC AGGACATGTT ATTGGAACAT 
GGCATTACTT TGGTCATTAA TGTAAAGTCA CAAGTTTTGG AACGAATCAG TCGAATGACC 
CAAGGTGATT TAGTGATGTC AATGGACCAG CTGCTTACGA AACCACACCT GGGCACTTGT 
CACAAATTTT ATATGCAGAT ATTTCAGTTG CCTAATGAAC AAACCAAGAC ACTGATGTTT 
TTTGAAGGTT GTCCACAGCA CCTAGGCTGT ACAATCAAGC TAAGAGGAGG CTCTGATTAT 
GAGCTGGCTC GAGTTAAGGA GATCCTAATA TTTATGATCT GTGTTGCTTA TCATTCTCAA 
CTAGAAATAT CCTTTCTCAT GGATGAATTT GCTATGCCTC CCACATTAAT GCAAAACCCT 
TCATTCCATT CCCTGATTGA GGGACGAGGG CATGAGGGGG CTGTCCAAGA GCAGTACGGT 
GGAGGTTCCA TCCCCTGGGA TCCTGACATC CCTCCTGAGT CTCTGCCCTG TGATGATAGC 
AGTTTGCTGG AATTGAGGAT TGTGTTTGAG AAGGGTGAGC AGGAAAATAA AAATCTTCCG 
CAGGCTGTTG CCTCTGTGAA GCATCAAGAA CATAGCACAA CAGCTTGCCC GGCGGGTCTC 
CCTTGTGCTT TCTTTGCACC TGTACCGGAA TCATTGTTGC CACTCCCTGT GGATGACCAA 
CAAGATGCTT TAGGCAGCGA GCAGCCAGAG ACTTTGCAGC AAACAGTTGT GCTGCAGGAT 
CCCAAAAGCC AGATAAGAGC CTTTAGAGAC CCTCTACAGG ATGACACTGG ATTATATGTT 
ACTGAGGAAG TCACCTCCTC TGAAGATAAA CGAAAGACTT ATTCTTTGGC CTTTAAGCAG 
GAATTAAAAG ATGTGATCCT CTGTATCTCC CCAGTAATCA CATTCCGAGA ACCCTTTCTT 
TTAACTGAAA AGGGGATGAG ATGCTCTACC CGAGATTATT TTGCAGAGCA GGTTTACTGG 
TCTCCTCTCC TCAATAAAGA ATTCAAAGAA ATGGAGAACA GGAGGAAGAA ACAGCTGCTC 
AGGGATCTCT CTGGACTTCA GGGCATGAAT GGAAGTATTC AGGCCAAGTC TATTCAAGTC 
TTACCCTCAC ATGAGCTAGT GAGCACTAGA ATTGCTGAGC ATCTGGGCGA TAGCCAGAGC 
TTGGGTAGAA TGCTGGCCGA TTATCGAGCC AGAGGAGGAA GAATTCAGCC CAAAAATTCA 
GACCCTTTTG CTCATTCAAA GGATGCATCA AGTACTTCAA GTGGCCAATC AGGAAGCAAA 
AATGAGGGTG ATGAAGAGAG AGGGCTTATT CTGAGTGATG CTGTGTGGTC AACAAAGGTG 
GACTGTCTGA ATCCCATTAA TCACCAGAGA CTTTGTGTGC TCTTCAGCAG CTCTTCTGCC 
CAGTCCAGCA ATGCTCCTAG TGCCTGTGTC AGTCCTTGGA TTGTAACAAT GGAATTTTAT 
GGAAAGAATG ATCTTACATT AGGAATATTT TTAGAGAGAT ACTGTTTCAG GCCTTCTTAT 
CAGTGTCCAA GCATGTTCTG TGATACCCCC ATGGTACATC ATATTCGGCG CTTTGTTCAT 
GGCCAAGGCT GTGTGCAGAT AATCCTGAAG GAGTTGGATT CTCCAGTACC TGGATATCAG 
CATACAATTC TTACATATTC CTGGTGTAGA ATCTGCAAAC AGGTAACACC AGTTGTTGCT 
CTTTCCAATG AGTCCTGGTC TATGTCATTT GCAAAATACC TTGAACTTAG GTTTTATGGG 
CACCAGTATA CTCGCAGAGC CAACGCTGAG CCCTGTGGTC ACTCCATCCA TCATGATTAT 
CACCAGTATT TCTCCTATAA CCAGATGGTG GCGTCTTTCA GTTATTCTCC CATTCGGCTT 
CTTGAAGTAT GTGTTCCACT CCCCAAAATA TTCATTAAGC GTCAGGCCCC ATTAAAAGTG 
TCCCTTCTTC AGGATCTGAA GGACTTCTTT CAAAAAGTTT CACAGGTATA TGTTGCCATT 
GATGAAAGAC TTGCATCTTT GAAAACTGAT ACATTTAGTA AAACAAGAGA GGAAAAAATG 
GAAGATATTT TTGCACAGAA AGAGATGGAA GAAGGTGAGT TCAAGAACTG GATTGAGAAG 
ATGCAAGCAA GGCTCATGTC TTCCTCTGTA GATACCCCTC AGCAACTGCA GTCGGTCTTT 
GAGTCACTCA TTGCCAAGAA ACAAAGTCTC TGTGAAGTGC TGCAAGCTTG GAATAACAGG 
TTGCAGGACC TTTTCCAACA GGAAAAGGGT AGAAAGAGAC CTTCAGTTCC TCCAAGTCCT 
GGAAGACTGA GACAAGGGGA AGAAAGCAAG ATAAGTGCGA TGGATGCATC TCCACGGAAT 
ATTTCTCCAG GACTTCAGAA TGGAGAAAAA GAGGATCGCT TCTTAACAAC TTTGTCCAGC 
CAGAGCTCCA CCAGTTCTAC TCATCTCCAA TTGCCTACGC CACCTGAAGT CATGTCTGAA 
CAGTCAGTGG GAGGGCCCCC TGAGCTAGAT ACAGCCAGCA GTTCCGAAGA TGTGTTTGAT 
GGGCATTTGC TGGGATCCAC AGACAGCCAA GTGAAGGAAA AGTCAACCAT GAAAGCCATC 
TTTGCAAATT TGCTTCCAGG AAATAGCTAT AATCCTATTC CATTTCCTTT TGATCCAGAT 
AAACACTACT TAATGTATGA ACATGAACGA GTGCCCATTG CAGTCTGCGA GAAGGAACCC 
AGCTCCATCA TTGCTTTTGC TCTCAGTTGT AAAGAATACC GAAATGCCTT AGAGGAATTG 
TCTAAAGCGA CTCAGTGGAA CAGTGCCGAA GAAGGGCTTC CAACAAATAG TACTTCAGAT 
AGCAGACCAA AGAGTAGCAG CCCTATCAGA TTACCTGAAA TGAGTGGAGG ACAGACAAAT 
CGTACAACAG AAACAGAACC ACAACCAACC AAAAAGGCTT CTGGAATGTT GTCCTTCTTC 
AGAGGGACAG CAGGGAAAAG CCCCGATCTC TCTTCCCAGA AGAGAGAGAC CTTACGTGGA 
GCAGATAGTG CTTACTACCA GGTTGGGCAG ACGGGCAAGG AGGGGACCGA GAATCAAGGC 
GTTGAGCCTC AAGATGAAGT AGATGGAGGA GATACACAAA AGAAGCAACT CATAAATCCT 
CATGTGGAAC TTCAATTTTC AGATGCTAAT GCCAAGTTTT ACTGTCGGCT CTACTATGCG 
GGAGAGTTTC ATAAGATGCG TGAAGTGATT CTGGACAGCA GTGAAGAAGA TTTCATTCGT 
TCCCTCTCCC ACTCATCACC CTGGCAGGCC CGGGGAGGCA AATCAGGAGC TGCCTTCTAT 
GCAACTGAGG ATGATAGATT TATTTTGAAG CAAATGCCTC GTCTGGAAGT CCAGTCCTTC 
CTCGACTTTG CACCACATTA CTTCAATTAT ATTACAAATG CTGTTCAACA AAAGAGGCCC 
ACGGCGTTGG CCAAAATTCT TGGAGTTTAC AGAATTGGTT ATAAGAACTC TCAGAACAAC 
ACTGAGAAGA AGTTAGATCT CCTTGTCATG GAAAATCTTT TCTACGGGAG AAAGATGGCA 
CAGGTTTTTG ATTTGAAGGG CTCTCTTAGG AATCGGAATG TAAAAACTGA CACTGGAAAA 
GAGAGTTGTG ATGTGGTCCT GCTAGATGAA AATCTCCTAA AGATGGTTCG AGACAACCCT 
CTATATATTC GTTCTCATTC CAAAGCTGTG CTGAGAACCT CGATCCATAG TGACTCCCAT 
TTCCTTTCTA GCCACCTCAT TATAGATTAT TCTTTGCTGG TTGGGCGAGA TGATACTAGC 
AATGAGCTAG TAGTTGGAAT TATAGATTAT ATTCGAACAT TTACATGGGA CAAAAAGCTT 
GAGATGGTTG TGAAATCAAC AGGAATTTTA GGTGGACAAG GTAAAATGCC AACAGTGGTG 
TCTCCGGAGT TGTACAGGAC TAGGTTTTGT GAGGCAATGG ACAAGTATTT CCTAATGGTA 
CCAGACCACT GGACAGGCTT GGGTCTGAAT TGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011509091.1
CDS	131..5986
Translation	MATDDKTSPTLDSANDLPRSPTSPSHLTHFKPLTPDQDEPPFKSAYSSFVNLFRFNKGDLRACTYCRKIALSYAHSTDSNSIGEDLNALSDSACSVSVLDPSEPRTPVGSRKASRNIFLEDDLAWQSLIHPDSSNTPLSTRLVSVQEDAGKSPARNRSASITNLSLDRSGSPMVPSYETSVSPQANRTYVRTETTEDERKILLDSVQLKDLWKKICHHSSGMEFQDHRYWLRTHPNCIVGKELVNWLIRNGHIATRAQAIAIGQAMVDGRWLDCVSHHDQLFRDEYALYRPLQSTEFSETPSPDSDSVNSVEGHSEPSWFKDIKFDDSDTEQIAEEGDDNLANSASPSKRTSVSSFQSTVDSDSAASISLNVELDNVNFHIKKPSKYPHVPPHPADQKEYLISDTGGQQLSISDAFIKESLFNRRVEEKSKELPFTPLGWHHNNLELLREENGEKQAMERLLSANHNHMMALLQQLLHSDSLSSSWRDIIVSLVCQVVQTVRPDVKNQDDDMDIRQFVHIKKIPGGKKFDSVVVNGFVCTKNIAHKKMSSCIKNPKILLLKCSIEYLYREETKFTCIDPIVLQEREFLKNYVQRIVDVRPTLVLVEKTVSRIAQDMLLEHGITLVINVKSQVLERISRMTQGDLVMSMDQLLTKPHLGTCHKFYMQIFQLPNEQTKTLMFFEGCPQHLGCTIKLRGGSDYELARVKEILIFMICVAYHSQLEISFLMDEFAMPPTLMQNPSFHSLIEGRGHEGAVQEQYGGGSIPWDPDIPPESLPCDDSSLLELRIVFEKGEQENKNLPQAVASVKHQEHSTTACPAGLPCAFFAPVPESLLPLPVDDQQDALGSEQPETLQQTVVLQDPKSQIRAFRDPLQDDTGLYVTEEVTSSEDKRKTYSLAFKQELKDVILCISPVITFREPFLLTEKGMRCSTRDYFAEQVYWSPLLNKEFKEMENRRKKQLLRDLSGLQGMNGSIQAKSIQVLPSHELVSTRIAEHLGDSQSLGRMLADYRARGGRIQPKNSDPFAHSKDASSTSSGQSGSKNEGDEERGLILSDAVWSTKVDCLNPINHQRLCVLFSSSSAQSSNAPSACVSPWIVTMEFYGKNDLTLGIFLERYCFRPSYQCPSMFCDTPMVHHIRRFVHGQGCVQIILKELDSPVPGYQHTILTYSWCRICKQVTPVVALSNESWSMSFAKYLELRFYGHQYTRRANAEPCGHSIHHDYHQYFSYNQMVASFSYSPIRLLEVCVPLPKIFIKRQAPLKVSLLQDLKDFFQKVSQVYVAIDERLASLKTDTFSKTREEKMEDIFAQKEMEEGEFKNWIEKMQARLMSSSVDTPQQLQSVFESLIAKKQSLCEVLQAWNNRLQDLFQQEKGRKRPSVPPSPGRLRQGEESKISAMDASPRNISPGLQNGEKEDRFLTTLSSQSSTSSTHLQLPTPPEVMSEQSVGGPPELDTASSSEDVFDGHLLGSTDSQVKEKSTMKAIFANLLPGNSYNPIPFPFDPDKHYLMYEHERVPIAVCEKEPSSIIAFALSCKEYRNALEELSKATQWNSAEEGLPTNSTSDSRPKSSSPIRLPEMSGGQTNRTTETEPQPTKKASGMLSFFRGTAGKSPDLSSQKRETLRGADSAYYQVGQTGKEGTENQGVEPQDEVDGGDTQKKQLINPHVELQFSDANAKFYCRLYYAGEFHKMREVILDSSEEDFIRSLSHSSPWQARGGKSGAAFYATEDDRFILKQMPRLEVQSFLDFAPHYFNYITNAVQQKRPTALAKILGVYRIGYKNSQNNTEKKLDLLVMENLFYGRKMAQVFDLKGSLRNRNVKTDTGKESCDVVLLDENLLKMVRDNPLYIRSHSKAVLRTSIHSDSHFLSSHLIIDYSLLVGRDDTSNELVVGIIDYIRTFTWDKKLEMVVKSTGILGGQGKMPTVVSPELYRTRFCEAMDKYFLMVPDHWTGLGLNC

Target ORF information:

RefSeq Version	XM_011510789.2
Organism	Homo sapiens(human)
Definition	Homo sapiens phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011510789.2

ORF Insert Sequence:

ATGGCCACAG ATGATAAGAC GTCCCCAACA CTGGACTCTG CTAATGATTT GCCTCGATCT 
CCTACTAGTC CTTCTCATCT CACACACTTT AAACCTTTGA CTCCTGATCA AGATGAGCCC 
CCTTTTAAAT CAGCTTATAG TTCTTTTGTA AATCTCTTTC GTTTTAACAA AGGAGACCTC 
CGAGCTTGCA CATATTGTAG AAAAATAGCC TTAAGTTATG CTCATTCCAC AGACAGTAAT 
TCTATTGGGG AAGACTTGAA TGCTCTTTCA GATTCTGCTT GCTCTGTGTC TGTGCTTGAT 
CCAAGTGAAC CCCGAACACC TGTTGGGAGT AGGAAAGCCA GCCGTAACAT ATTTTTAGAG 
GATGATTTGG CCTGGCAAAG TTTGATTCAT CCAGATTCCT CAAATACTCC TCTTTCAACA 
AGACTTGTAT CTGTGCAAGA GGATGCTGGG AAATCTCCTG CTCGAAATAG ATCAGCCAGC 
ATTACTAACC TGTCACTGGA TAGATCTGGT TCTCCTATGG TACCTTCATA TGAGACATCT 
GTCAGTCCCC AGGCTAACCG AACATATGTT AGGACAGAGA CCACTGAGGA TGAACGCAAA 
ATTCTTCTGG ACAGTGTGCA GTTAAAAGAC CTGTGGAAAA AAATCTGCCA TCACAGCAGT 
GGAATGGAGT TTCAGGATCA CCGCTACTGG TTGAGAACGC ATCCCAACTG CATTGTAGGA 
AAGGAATTAG TCAACTGGCT AATCCGAAAT GGGCATATTG CCACAAGGGC ACAAGCTATA 
GCAATTGGAC AAGCAATGGT TGATGGACGT TGGCTGGATT GTGTTAGTCA TCACGACCAG 
CTTTTCAGAG ATGAGTATGC GCTGTATAGA CCACTGCAGA GTACAGAATT TTCTGAGACG 
CCTTCTCCCG ACAGTGACTC AGTGAACTCC GTGGAAGGAC ACTCTGAGCC ATCCTGGTTT 
AAAGACATAA AGTTTGATGA CAGTGACACA GAACAGATAG CTGAAGAAGG TGACGATAAT 
TTGGCTAATT CTGCCAGTCC TAGCAAGCGC ACATCAGTCA GCAGTTTCCA GTCCACAGTG 
GACAGTGACT CAGCCGCTTC TATCAGCCTG AACGTGGAGC TGGACAACGT GAACTTCCAT 
ATCAAGAAGC CCTCCAAGTA CCCACATGTG CCCCCTCACC CTGCTGACCA AAAAGAGTAT 
TTGATTTCTG ACACTGGAGG ACAACAGCTC TCAATAAGTG ACGCTTTCAT CAAAGAATCC 
TTATTTAATC GCCGAGTAGA GGAAAAATCC AAAGAGCTGC CTTTCACACC TTTGGGCTGG 
CATCATAACA ACCTGGAGCT CCTGAGGGAG GAGAATGGGG AGAAACAAGC CATGGAGAGG 
TTGCTTTCAG CTAATCATAA CCACATGATG GCACTACTCC AGCAGTTGCT CCATAGTGAC 
TCACTGTCAT CATCTTGGAG GGACATCATC GTGTCATTGG TCTGCCAGGT TGTTCAGACA 
GTCCGACCTG ATGTCAAGAA CCAGGATGAT GACATGGATA TCCGTCAGTT TGTCCACATC 
AAAAAAATCC CAGGTGGAAA GAAGTTTGAT TCTGTGGTTG TCAATGGCTT TGTTTGTACC 
AAGAACATTG CACATAAAAA GATGAGTTCT TGTATTAAAA ACCCCAAAAT TCTTCTGTTG 
AAGTGTTCCA TTGAGTATCT CTACAGAGAA GAAACTAAGT TTACTTGCAT TGATCCTATT 
GTGCTTCAGG AAAGGGAATT CTTGAAGAAT TATGTCCAGC GAATAGTTGA TGTTCGACCC 
ACCTTGGTTC TTGTTGAGAA AACAGTGTCT CGGATTGCCC AGGACATGTT ATTGGAACAT 
GGCATTACTT TGGTCATTAA TGTAAAGTCA CAAGTTTTGG AACGAATCAG TCGAATGACC 
CAAGGTGATT TAGTGATGTC AATGGACCAG CTGCTTACGA AACCACACCT GGGCACTTGT 
CACAAATTTT ATATGCAGAT ATTTCAGTTG CCTAATGAAC AAACCAAGAC ACTGATGTTT 
TTTGAAGGTT GTCCACAGCA CCTAGGCTGT ACAATCAAGC TAAGAGGAGG CTCTGATTAT 
GAGCTGGCTC GAGTTAAGGA GATCCTAATA TTTATGATCT GTGTTGCTTA TCATTCTCAA 
CTAGAAATAT CCTTTCTCAT GGATGAATTT GCTATGCCTC CCACATTAAT GCAAAACCCT 
TCATTCCATT CCCTGATTGA GGGACGAGGG CATGAGGGGG CTGTCCAAGA GCAGTACGGT 
GGAGGTTCCA TCCCCTGGGA TCCTGACATC CCTCCTGAGT CTCTGCCCTG TGATGATAGC 
AGTTTGCTGG AATTGAGGAT TGTGTTTGAG AAGGGTGAGC AGGAAAATAA AAATCTTCCG 
CAGGCTGTTG CCTCTGTGAA GCATCAAGAA CATAGCACAA CAGCTTGCCC GGCGGGTCTC 
CCTTGTGCTT TCTTTGCACC TGTACCGGAA TCATTGTTGC CACTCCCTGT GGATGACCAA 
CAAGATGCTT TAGGCAGCGA GCAGCCAGAG ACTTTGCAGC AAACAGTTGT GCTGCAGGAT 
CCCAAAAGCC AGATAAGAGC CTTTAGAGAC CCTCTACAGG ATGACACTGG ATTATATGTT 
ACTGAGGAAG TCACCTCCTC TGAAGATAAA CGAAAGACTT ATTCTTTGGC CTTTAAGCAG 
GAATTAAAAG ATGTGATCCT CTGTATCTCC CCAGTAATCA CATTCCGAGA ACCCTTTCTT 
TTAACTGAAA AGGGGATGAG ATGCTCTACC CGAGATTATT TTGCAGAGCA GGTTTACTGG 
TCTCCTCTCC TCAATAAAGA ATTCAAAGAA ATGGAGAACA GGAGGAAGAA ACAGCTGCTC 
AGGGATCTCT CTGGACTTCA GGGCATGAAT GGAAGTATTC AGGCCAAGTC TATTCAAGTC 
TTACCCTCAC ATGAGCTAGT GAGCACTAGA ATTGCTGAGC ATCTGGGCGA TAGCCAGAGC 
TTGGGTAGAA TGCTGGCCGA TTATCGAGCC AGAGGAGGAA GAATTCAGCC CAAAAATTCA 
GACCCTTTTG CTCATTCAAA GGATGCATCA AGTACTTCAA GTGGCCAATC AGGAAGCAAA 
AATGAGGGTG ATGAAGAGAG AGGGCTTATT CTGAGTGATG CTGTGTGGTC AACAAAGGTG 
GACTGTCTGA ATCCCATTAA TCACCAGAGA CTTTGTGTGC TCTTCAGCAG CTCTTCTGCC 
CAGTCCAGCA ATGCTCCTAG TGCCTGTGTC AGTCCTTGGA TTGTAACAAT GGAATTTTAT 
GGAAAGAATG ATCTTACATT AGGAATATTT TTAGAGAGAT ACTGTTTCAG GCCTTCTTAT 
CAGTGTCCAA GCATGTTCTG TGATACCCCC ATGGTACATC ATATTCGGCG CTTTGTTCAT 
GGCCAAGGCT GTGTGCAGAT AATCCTGAAG GAGTTGGATT CTCCAGTACC TGGATATCAG 
CATACAATTC TTACATATTC CTGGTGTAGA ATCTGCAAAC AGGTAACACC AGTTGTTGCT 
CTTTCCAATG AGTCCTGGTC TATGTCATTT GCAAAATACC TTGAACTTAG GTTTTATGGG 
CACCAGTATA CTCGCAGAGC CAACGCTGAG CCCTGTGGTC ACTCCATCCA TCATGATTAT 
CACCAGTATT TCTCCTATAA CCAGATGGTG GCGTCTTTCA GTTATTCTCC CATTCGGCTT 
CTTGAAGTAT GTGTTCCACT CCCCAAAATA TTCATTAAGC GTCAGGCCCC ATTAAAAGTG 
TCCCTTCTTC AGGATCTGAA GGACTTCTTT CAAAAAGTTT CACAGGTATA TGTTGCCATT 
GATGAAAGAC TTGCATCTTT GAAAACTGAT ACATTTAGTA AAACAAGAGA GGAAAAAATG 
GAAGATATTT TTGCACAGAA AGAGATGGAA GAAGGTGAGT TCAAGAACTG GATTGAGAAG 
ATGCAAGCAA GGCTCATGTC TTCCTCTGTA GATACCCCTC AGCAACTGCA GTCGGTCTTT 
GAGTCACTCA TTGCCAAGAA ACAAAGTCTC TGTGAAGTGC TGCAAGCTTG GAATAACAGG 
TTGCAGGACC TTTTCCAACA GGAAAAGGGT AGAAAGAGAC CTTCAGTTCC TCCAAGTCCT 
GGAAGACTGA GACAAGGGGA AGAAAGCAAG ATAAGTGCGA TGGATGCATC TCCACGGAAT 
ATTTCTCCAG GACTTCAGAA TGGAGAAAAA GAGGATCGCT TCTTAACAAC TTTGTCCAGC 
CAGAGCTCCA CCAGTTCTAC TCATCTCCAA TTGCCTACGC CACCTGAAGT CATGTCTGAA 
CAGTCAGTGG GAGGGCCCCC TGAGCTAGAT ACAGCCAGCA GTTCCGAAGA TGTGTTTGAT 
GGGCATTTGC TGGGATCCAC AGACAGCCAA GTGAAGGAAA AGTCAACCAT GAAAGCCATC 
TTTGCAAATT TGCTTCCAGG AAATAGCTAT AATCCTATTC CATTTCCTTT TGATCCAGAT 
AAACACTACT TAATGTATGA ACATGAACGA GTGCCCATTG CAGTCTGCGA GAAGGAACCC 
AGCTCCATCA TTGCTTTTGC TCTCAGTTGT AAAGAATACC GAAATGCCTT AGAGGAATTG 
TCTAAAGCGA CTCAGTGGAA CAGTGCCGAA GAAGGGCTTC CAACAAATAG TACTTCAGAT 
AGCAGACCAA AGAGTAGCAG CCCTATCAGA TTACCTGAAA TGAGTGGAGG ACAGACAAAT 
CGTACAACAG AAACAGAACC ACAACCAACC AAAAAGGCTT CTGGAATGTT GTCCTTCTTC 
AGAGGGACAG CAGGGAAAAG CCCCGATCTC TCTTCCCAGA AGAGAGAGAC CTTACGTGGA 
GCAGATAGTG CTTACTACCA GGTTGGGCAG ACGGGCAAGG AGGGGACCGA GAATCAAGGC 
GTTGAGCCTC AAGATGAAGT AGATGGAGGA GATACACAAA AGAAGCAACT CATAAATCCT 
CATGTGGAAC TTCAATTTTC AGATGCTAAT GCCAAGTTTT ACTGTCGGCT CTACTATGCG 
GGAGAGTTTC ATAAGATGCG TGAAGTGATT CTGGACAGCA GTGAAGAAGA TTTCATTCGT 
TCCCTCTCCC ACTCATCACC CTGGCAGGCC CGGGGAGGCA AATCAGGAGC TGCCTTCTAT 
GCAACTGAGG ATGATAGATT TATTTTGAAG CAAATGCCTC GTCTGGAAGT CCAGTCCTTC 
CTCGACTTTG CACCACATTA CTTCAATTAT ATTACAAATG CTGTTCAACA AAAGAGGCCC 
ACGGCGTTGG CCAAAATTCT TGGAGTTTAC AGAATTGGTT ATAAGAACTC TCAGAACAAC 
ACTGAGAAGA AGTTAGATCT CCTTGTCATG GAAAATCTTT TCTACGGGAG AAAGATGGCA 
CAGGTTTTTG ATTTGAAGGG CTCTCTTAGG AATCGGAATG TAAAAACTGA CACTGGAAAA 
GAGAGTTGTG ATGTGGTCCT GCTAGATGAA AATCTCCTAA AGATGGTTCG AGACAACCCT 
CTATATATTC GTTCTCATTC CAAAGCTGTG CTGAGAACCT CGATCCATAG TGACTCCCAT 
TTCCTTTCTA GCCACCTCAT TATAGATTAT TCTTTGCTGG TTGGGCGAGA TGATACTAGC 
AATGAGCTAG TAGTTGGAAT TATAGATTAT ATTCGAACAT TTACATGGGA CAAAAAGCTT 
GAGATGGTTG TGAAATCAAC AGGAATTTTA GGTGGACAAG GTAAAATGCC AACAGTGGTG 
TCTCCGGAGT TGTACAGGAC TAGGTTTTGT GAGGCAATGG ACAAGTATTT CCTAATGGTA 
CCAGACCACT GGACAGGCTT GGGTCTGAAT TGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PIKFYVE ( XM_011510790.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011509092.1 Homo sapiens phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X17, mRNA.

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CloneID	OHu77672
Clone ID Related Accession (Same CDS sequence)	XM_011510790.1 , XM_017003572.1 , XM_017003573.1 , XM_017003574.1
Accession Version	XM_011510790.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5340bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	1-phosphatidylinositol 3-phosphate 5-kinase isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTACCTT CATATGAGAC ATCTGTCAGT CCCCAGGCTA ACCGAACATA TGTTAGGACA 
GAGACCACTG AGGATGAACG CAAAATTCTT CTGGACAGTG TGCAGTTAAA AGACCTGTGG 
AAAAAAATCT GCCATCACAG CAGTGGAATG GAGTTTCAGG ATCACCGCTA CTGGTTGAGA 
ACGCATCCCA ACTGCATTGT AGGAAAGGAA TTAGTCAACT GGCTAATCCG AAATGGGCAT 
ATTGCCACAA GGGCACAAGC TATAGCAATT GGACAAGCAA TGGTTGATGG ACGTTGGCTG 
GATTGTGTTA GTCATCACGA CCAGCTTTTC AGAGATGAGT ATGCGCTGTA TAGACCACTG 
CAGAGTACAG AATTTTCTGA GACGCCTTCT CCCGACAGTG ACTCAGTGAA CTCCGTGGAA 
GGACACTCTG AGCCATCCTG GTTTAAAGAC ATAAAGTTTG ATGACAGTGA CACAGAACAG 
ATAGCTGAAG AAGGTGACGA TAATTTGGCT AATTCTGCCA GTCCTAGCAA GCGCACATCA 
GTCAGCAGTT TCCAGTCCAC AGTGGACAGT GACTCAGCCG CTTCTATCAG CCTGAACGTG 
GAGCTGGACA ACGTGAACTT CCATATCAAG AAGCCCTCCA AGTACCCACA TGTGCCCCCT 
CACCCTGCTG ACCAAAAAGA GTATTTGATT TCTGACACTG GAGGACAACA GCTCTCAATA 
AGTGACGCTT TCATCAAAGA ATCCTTATTT AATCGCCGAG TAGAGGAAAA ATCCAAAGAG 
CTGCCTTTCA CACCTTTGGG CTGGCATCAT AACAACCTGG AGCTCCTGAG GGAGGAGAAT 
GGGGAGAAAC AAGCCATGGA GAGGTTGCTT TCAGCTAATC ATAACCACAT GATGGCACTA 
CTCCAGCAGT TGCTCCATAG TGACTCACTG TCATCATCTT GGAGGGACAT CATCGTGTCA 
TTGGTCTGCC AGGTTGTTCA GACAGTCCGA CCTGATGTCA AGAACCAGGA TGATGACATG 
GATATCCGTC AGTTTGTCCA CATCAAAAAA ATCCCAGGTG GAAAGAAGTT TGATTCTGTG 
GTTGTCAATG GCTTTGTTTG TACCAAGAAC ATTGCACATA AAAAGATGAG TTCTTGTATT 
AAAAACCCCA AAATTCTTCT GTTGAAGTGT TCCATTGAGT ATCTCTACAG AGAAGAAACT 
AAGTTTACTT GCATTGATCC TATTGTGCTT CAGGAAAGGG AATTCTTGAA GAATTATGTC 
CAGCGAATAG TTGATGTTCG ACCCACCTTG GTTCTTGTTG AGAAAACAGT GTCTCGGATT 
GCCCAGGACA TGTTATTGGA ACATGGCATT ACTTTGGTCA TTAATGTAAA GTCACAAGTT 
TTGGAACGAA TCAGTCGAAT GACCCAAGGT GATTTAGTGA TGTCAATGGA CCAGCTGCTT 
ACGAAACCAC ACCTGGGCAC TTGTCACAAA TTTTATATGC AGATATTTCA GTTGCCTAAT 
GAACAAACCA AGACACTGAT GTTTTTTGAA GGTTGTCCAC AGCACCTAGG CTGTACAATC 
AAGCTAAGAG GAGGCTCTGA TTATGAGCTG GCTCGAGTTA AGGAGATCCT AATATTTATG 
ATCTGTGTTG CTTATCATTC TCAACTAGAA ATATCCTTTC TCATGGATGA ATTTGCTATG 
CCTCCCACAT TAATGCAAAA CCCTTCATTC CATTCCCTGA TTGAGGGACG AGGGCATGAG 
GGGGCTGTCC AAGAGCAGTA CGGTGGAGGT TCCATCCCCT GGGATCCTGA CATCCCTCCT 
GAGTCTCTGC CCTGTGATGA TAGCAGTTTG CTGGAATTGA GGATTGTGTT TGAGAAGGGT 
GAGCAGGAAA ATAAAAATCT TCCGCAGGCT GTTGCCTCTG TGAAGCATCA AGAACATAGC 
ACAACAGCTT GCCCGGCGGG TCTCCCTTGT GCTTTCTTTG CACCTGTACC GGAATCATTG 
TTGCCACTCC CTGTGGATGA CCAACAAGAT GCTTTAGGCA GCGAGCAGCC AGAGACTTTG 
CAGCAAACAG TTGTGCTGCA GGATCCCAAA AGCCAGATAA GAGCCTTTAG AGACCCTCTA 
CAGGATGACA CTGGATTATA TGTTACTGAG GAAGTCACCT CCTCTGAAGA TAAACGAAAG 
ACTTATTCTT TGGCCTTTAA GCAGGAATTA AAAGATGTGA TCCTCTGTAT CTCCCCAGTA 
ATCACATTCC GAGAACCCTT TCTTTTAACT GAAAAGGGGA TGAGATGCTC TACCCGAGAT 
TATTTTGCAG AGCAGGTTTA CTGGTCTCCT CTCCTCAATA AAGAATTCAA AGAAATGGAG 
AACAGGAGGA AGAAACAGCT GCTCAGGGAT CTCTCTGGAC TTCAGGGCAT GAATGGAAGT 
ATTCAGGCCA AGTCTATTCA AGTCTTACCC TCACATGAGC TAGTGAGCAC TAGAATTGCT 
GAGCATCTGG GCGATAGCCA GAGCTTGGGT AGAATGCTGG CCGATTATCG AGCCAGAGGA 
GGAAGAATTC AGCCCAAAAA TTCAGACCCT TTTGCTCATT CAAAGGATGC ATCAAGTACT 
TCAAGTGGCC AATCAGGAAG CAAAAATGAG GGTGATGAAG AGAGAGGGCT TATTCTGAGT 
GATGCTGTGT GGTCAACAAA GGTGGACTGT CTGAATCCCA TTAATCACCA GAGACTTTGT 
GTGCTCTTCA GCAGCTCTTC TGCCCAGTCC AGCAATGCTC CTAGTGCCTG TGTCAGTCCT 
TGGATTGTAA CAATGGAATT TTATGGAAAG AATGATCTTA CATTAGGAAT ATTTTTAGAG 
AGATACTGTT TCAGGCCTTC TTATCAGTGT CCAAGCATGT TCTGTGATAC CCCCATGGTA 
CATCATATTC GGCGCTTTGT TCATGGCCAA GGCTGTGTGC AGATAATCCT GAAGGAGTTG 
GATTCTCCAG TACCTGGATA TCAGCATACA ATTCTTACAT ATTCCTGGTG TAGAATCTGC 
AAACAGGTAA CACCAGTTGT TGCTCTTTCC AATGAGTCCT GGTCTATGTC ATTTGCAAAA 
TACCTTGAAC TTAGGTTTTA TGGGCACCAG TATACTCGCA GAGCCAACGC TGAGCCCTGT 
GGTCACTCCA TCCATCATGA TTATCACCAG TATTTCTCCT ATAACCAGAT GGTGGCGTCT 
TTCAGTTATT CTCCCATTCG GCTTCTTGAA GTATGTGTTC CACTCCCCAA AATATTCATT 
AAGCGTCAGG CCCCATTAAA AGTGTCCCTT CTTCAGGATC TGAAGGACTT CTTTCAAAAA 
GTTTCACAGG TATATGTTGC CATTGATGAA AGACTTGCAT CTTTGAAAAC TGATACATTT 
AGTAAAACAA GAGAGGAAAA AATGGAAGAT ATTTTTGCAC AGAAAGAGAT GGAAGAAGGT 
GAGTTCAAGA ACTGGATTGA GAAGATGCAA GCAAGGCTCA TGTCTTCCTC TGTAGATACC 
CCTCAGCAAC TGCAGTCGGT CTTTGAGTCA CTCATTGCCA AGAAACAAAG TCTCTGTGAA 
GTGCTGCAAG CTTGGAATAA CAGGTTGCAG GACCTTTTCC AACAGGAAAA GGGTAGAAAG 
AGACCTTCAG TTCCTCCAAG TCCTGGAAGA CTGAGACAAG GGGAAGAAAG CAAGATAAGT 
GCGATGGATG CATCTCCACG GAATATTTCT CCAGGACTTC AGAATGGAGA AAAAGAGGAT 
CGCTTCTTAA CAACTTTGTC CAGCCAGAGC TCCACCAGTT CTACTCATCT CCAATTGCCT 
ACGCCACCTG AAGTCATGTC TGAACAGTCA GTGGGAGGGC CCCCTGAGCT AGATACAGCC 
AGCAGTTCCG AAGATGTGTT TGATGGGCAT TTGCTGGGAT CCACAGACAG CCAAGTGAAG 
GAAAAGTCAA CCATGAAAGC CATCTTTGCA AATTTGCTTC CAGGAAATAG CTATAATCCT 
ATTCCATTTC CTTTTGATCC AGATAAACAC TACTTAATGT ATGAACATGA ACGAGTGCCC 
ATTGCAGTCT GCGAGAAGGA ACCCAGCTCC ATCATTGCTT TTGCTCTCAG TTGTAAAGAA 
TACCGAAATG CCTTAGAGGA ATTGTCTAAA GCGACTCAGT GGAACAGTGC CGAAGAAGGG 
CTTCCAACAA ATAGTACTTC AGATAGCAGA CCAAAGAGTA GCAGCCCTAT CAGATTACCT 
GAAATGAGTG GAGGACAGAC AAATCGTACA ACAGAAACAG AACCACAACC AACCAAAAAG 
GCTTCTGGAA TGTTGTCCTT CTTCAGAGGG ACAGCAGGGA AAAGCCCCGA TCTCTCTTCC 
CAGAAGAGAG AGACCTTACG TGGAGCAGAT AGTGCTTACT ACCAGGTTGG GCAGACGGGC 
AAGGAGGGGA CCGAGAATCA AGGCGTTGAG CCTCAAGATG AAGTAGATGG AGGAGATACA 
CAAAAGAAGC AACTCATAAA TCCTCATGTG GAACTTCAAT TTTCAGATGC TAATGCCAAG 
TTTTACTGTC GGCTCTACTA TGCGGGAGAG TTTCATAAGA TGCGTGAAGT GATTCTGGAC 
AGCAGTGAAG AAGATTTCAT TCGTTCCCTC TCCCACTCAT CACCCTGGCA GGCCCGGGGA 
GGCAAATCAG GAGCTGCCTT CTATGCAACT GAGGATGATA GATTTATTTT GAAGCAAATG 
CCTCGTCTGG AAGTCCAGTC CTTCCTCGAC TTTGCACCAC ATTACTTCAA TTATATTACA 
AATGCTGTTC AACAAAAGAG GCCCACGGCG TTGGCCAAAA TTCTTGGAGT TTACAGAATT 
GGTTATAAGA ACTCTCAGAA CAACACTGAG AAGAAGTTAG ATCTCCTTGT CATGGAAAAT 
CTTTTCTACG GGAGAAAGAT GGCACAGGTT TTTGATTTGA AGGGCTCTCT TAGGAATCGG 
AATGTAAAAA CTGACACTGG AAAAGAGAGT TGTGATGTGG TCCTGCTAGA TGAAAATCTC 
CTAAAGATGG TTCGAGACAA CCCTCTATAT ATTCGTTCTC ATTCCAAAGC TGTGCTGAGA 
ACCTCGATCC ATAGTGACTC CCATTTCCTT TCTAGCCACC TCATTATAGA TTATTCTTTG 
CTGGTTGGGC GAGATGATAC TAGCAATGAG CTAGTAGTTG GAATTATAGA TTATATTCGA 
ACATTTACAT GGGACAAAAA GCTTGAGATG GTTGTGAAAT CAACAGGAAT TTTAGGTGGA 
CAAGGTAAAA TGCCAACAGT GGTGTCTCCG GAGTTGTACA GGACTAGGTT TTGTGAGGCA 
ATGGACAAGT ATTTCCTAAT GGTACCAGAC CACTGGACAG GCTTGGGTCT GAATTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011509092.1
CDS	944..6283
Translation	MVPSYETSVSPQANRTYVRTETTEDERKILLDSVQLKDLWKKICHHSSGMEFQDHRYWLRTHPNCIVGKELVNWLIRNGHIATRAQAIAIGQAMVDGRWLDCVSHHDQLFRDEYALYRPLQSTEFSETPSPDSDSVNSVEGHSEPSWFKDIKFDDSDTEQIAEEGDDNLANSASPSKRTSVSSFQSTVDSDSAASISLNVELDNVNFHIKKPSKYPHVPPHPADQKEYLISDTGGQQLSISDAFIKESLFNRRVEEKSKELPFTPLGWHHNNLELLREENGEKQAMERLLSANHNHMMALLQQLLHSDSLSSSWRDIIVSLVCQVVQTVRPDVKNQDDDMDIRQFVHIKKIPGGKKFDSVVVNGFVCTKNIAHKKMSSCIKNPKILLLKCSIEYLYREETKFTCIDPIVLQEREFLKNYVQRIVDVRPTLVLVEKTVSRIAQDMLLEHGITLVINVKSQVLERISRMTQGDLVMSMDQLLTKPHLGTCHKFYMQIFQLPNEQTKTLMFFEGCPQHLGCTIKLRGGSDYELARVKEILIFMICVAYHSQLEISFLMDEFAMPPTLMQNPSFHSLIEGRGHEGAVQEQYGGGSIPWDPDIPPESLPCDDSSLLELRIVFEKGEQENKNLPQAVASVKHQEHSTTACPAGLPCAFFAPVPESLLPLPVDDQQDALGSEQPETLQQTVVLQDPKSQIRAFRDPLQDDTGLYVTEEVTSSEDKRKTYSLAFKQELKDVILCISPVITFREPFLLTEKGMRCSTRDYFAEQVYWSPLLNKEFKEMENRRKKQLLRDLSGLQGMNGSIQAKSIQVLPSHELVSTRIAEHLGDSQSLGRMLADYRARGGRIQPKNSDPFAHSKDASSTSSGQSGSKNEGDEERGLILSDAVWSTKVDCLNPINHQRLCVLFSSSSAQSSNAPSACVSPWIVTMEFYGKNDLTLGIFLERYCFRPSYQCPSMFCDTPMVHHIRRFVHGQGCVQIILKELDSPVPGYQHTILTYSWCRICKQVTPVVALSNESWSMSFAKYLELRFYGHQYTRRANAEPCGHSIHHDYHQYFSYNQMVASFSYSPIRLLEVCVPLPKIFIKRQAPLKVSLLQDLKDFFQKVSQVYVAIDERLASLKTDTFSKTREEKMEDIFAQKEMEEGEFKNWIEKMQARLMSSSVDTPQQLQSVFESLIAKKQSLCEVLQAWNNRLQDLFQQEKGRKRPSVPPSPGRLRQGEESKISAMDASPRNISPGLQNGEKEDRFLTTLSSQSSTSSTHLQLPTPPEVMSEQSVGGPPELDTASSSEDVFDGHLLGSTDSQVKEKSTMKAIFANLLPGNSYNPIPFPFDPDKHYLMYEHERVPIAVCEKEPSSIIAFALSCKEYRNALEELSKATQWNSAEEGLPTNSTSDSRPKSSSPIRLPEMSGGQTNRTTETEPQPTKKASGMLSFFRGTAGKSPDLSSQKRETLRGADSAYYQVGQTGKEGTENQGVEPQDEVDGGDTQKKQLINPHVELQFSDANAKFYCRLYYAGEFHKMREVILDSSEEDFIRSLSHSSPWQARGGKSGAAFYATEDDRFILKQMPRLEVQSFLDFAPHYFNYITNAVQQKRPTALAKILGVYRIGYKNSQNNTEKKLDLLVMENLFYGRKMAQVFDLKGSLRNRNVKTDTGKESCDVVLLDENLLKMVRDNPLYIRSHSKAVLRTSIHSDSHFLSSHLIIDYSLLVGRDDTSNELVVGIIDYIRTFTWDKKLEMVVKSTGILGGQGKMPTVVSPELYRTRFCEAMDKYFLMVPDHWTGLGLNC

Target ORF information:

RefSeq Version	XM_011510790.1
Organism	Homo sapiens(human)
Definition	Homo sapiens phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011510790.1

ORF Insert Sequence:

ATGGTACCTT CATATGAGAC ATCTGTCAGT CCCCAGGCTA ACCGAACATA TGTTAGGACA 
GAGACCACTG AGGATGAACG CAAAATTCTT CTGGACAGTG TGCAGTTAAA AGACCTGTGG 
AAAAAAATCT GCCATCACAG CAGTGGAATG GAGTTTCAGG ATCACCGCTA CTGGTTGAGA 
ACGCATCCCA ACTGCATTGT AGGAAAGGAA TTAGTCAACT GGCTAATCCG AAATGGGCAT 
ATTGCCACAA GGGCACAAGC TATAGCAATT GGACAAGCAA TGGTTGATGG ACGTTGGCTG 
GATTGTGTTA GTCATCACGA CCAGCTTTTC AGAGATGAGT ATGCGCTGTA TAGACCACTG 
CAGAGTACAG AATTTTCTGA GACGCCTTCT CCCGACAGTG ACTCAGTGAA CTCCGTGGAA 
GGACACTCTG AGCCATCCTG GTTTAAAGAC ATAAAGTTTG ATGACAGTGA CACAGAACAG 
ATAGCTGAAG AAGGTGACGA TAATTTGGCT AATTCTGCCA GTCCTAGCAA GCGCACATCA 
GTCAGCAGTT TCCAGTCCAC AGTGGACAGT GACTCAGCCG CTTCTATCAG CCTGAACGTG 
GAGCTGGACA ACGTGAACTT CCATATCAAG AAGCCCTCCA AGTACCCACA TGTGCCCCCT 
CACCCTGCTG ACCAAAAAGA GTATTTGATT TCTGACACTG GAGGACAACA GCTCTCAATA 
AGTGACGCTT TCATCAAAGA ATCCTTATTT AATCGCCGAG TAGAGGAAAA ATCCAAAGAG 
CTGCCTTTCA CACCTTTGGG CTGGCATCAT AACAACCTGG AGCTCCTGAG GGAGGAGAAT 
GGGGAGAAAC AAGCCATGGA GAGGTTGCTT TCAGCTAATC ATAACCACAT GATGGCACTA 
CTCCAGCAGT TGCTCCATAG TGACTCACTG TCATCATCTT GGAGGGACAT CATCGTGTCA 
TTGGTCTGCC AGGTTGTTCA GACAGTCCGA CCTGATGTCA AGAACCAGGA TGATGACATG 
GATATCCGTC AGTTTGTCCA CATCAAAAAA ATCCCAGGTG GAAAGAAGTT TGATTCTGTG 
GTTGTCAATG GCTTTGTTTG TACCAAGAAC ATTGCACATA AAAAGATGAG TTCTTGTATT 
AAAAACCCCA AAATTCTTCT GTTGAAGTGT TCCATTGAGT ATCTCTACAG AGAAGAAACT 
AAGTTTACTT GCATTGATCC TATTGTGCTT CAGGAAAGGG AATTCTTGAA GAATTATGTC 
CAGCGAATAG TTGATGTTCG ACCCACCTTG GTTCTTGTTG AGAAAACAGT GTCTCGGATT 
GCCCAGGACA TGTTATTGGA ACATGGCATT ACTTTGGTCA TTAATGTAAA GTCACAAGTT 
TTGGAACGAA TCAGTCGAAT GACCCAAGGT GATTTAGTGA TGTCAATGGA CCAGCTGCTT 
ACGAAACCAC ACCTGGGCAC TTGTCACAAA TTTTATATGC AGATATTTCA GTTGCCTAAT 
GAACAAACCA AGACACTGAT GTTTTTTGAA GGTTGTCCAC AGCACCTAGG CTGTACAATC 
AAGCTAAGAG GAGGCTCTGA TTATGAGCTG GCTCGAGTTA AGGAGATCCT AATATTTATG 
ATCTGTGTTG CTTATCATTC TCAACTAGAA ATATCCTTTC TCATGGATGA ATTTGCTATG 
CCTCCCACAT TAATGCAAAA CCCTTCATTC CATTCCCTGA TTGAGGGACG AGGGCATGAG 
GGGGCTGTCC AAGAGCAGTA CGGTGGAGGT TCCATCCCCT GGGATCCTGA CATCCCTCCT 
GAGTCTCTGC CCTGTGATGA TAGCAGTTTG CTGGAATTGA GGATTGTGTT TGAGAAGGGT 
GAGCAGGAAA ATAAAAATCT TCCGCAGGCT GTTGCCTCTG TGAAGCATCA AGAACATAGC 
ACAACAGCTT GCCCGGCGGG TCTCCCTTGT GCTTTCTTTG CACCTGTACC GGAATCATTG 
TTGCCACTCC CTGTGGATGA CCAACAAGAT GCTTTAGGCA GCGAGCAGCC AGAGACTTTG 
CAGCAAACAG TTGTGCTGCA GGATCCCAAA AGCCAGATAA GAGCCTTTAG AGACCCTCTA 
CAGGATGACA CTGGATTATA TGTTACTGAG GAAGTCACCT CCTCTGAAGA TAAACGAAAG 
ACTTATTCTT TGGCCTTTAA GCAGGAATTA AAAGATGTGA TCCTCTGTAT CTCCCCAGTA 
ATCACATTCC GAGAACCCTT TCTTTTAACT GAAAAGGGGA TGAGATGCTC TACCCGAGAT 
TATTTTGCAG AGCAGGTTTA CTGGTCTCCT CTCCTCAATA AAGAATTCAA AGAAATGGAG 
AACAGGAGGA AGAAACAGCT GCTCAGGGAT CTCTCTGGAC TTCAGGGCAT GAATGGAAGT 
ATTCAGGCCA AGTCTATTCA AGTCTTACCC TCACATGAGC TAGTGAGCAC TAGAATTGCT 
GAGCATCTGG GCGATAGCCA GAGCTTGGGT AGAATGCTGG CCGATTATCG AGCCAGAGGA 
GGAAGAATTC AGCCCAAAAA TTCAGACCCT TTTGCTCATT CAAAGGATGC ATCAAGTACT 
TCAAGTGGCC AATCAGGAAG CAAAAATGAG GGTGATGAAG AGAGAGGGCT TATTCTGAGT 
GATGCTGTGT GGTCAACAAA GGTGGACTGT CTGAATCCCA TTAATCACCA GAGACTTTGT 
GTGCTCTTCA GCAGCTCTTC TGCCCAGTCC AGCAATGCTC CTAGTGCCTG TGTCAGTCCT 
TGGATTGTAA CAATGGAATT TTATGGAAAG AATGATCTTA CATTAGGAAT ATTTTTAGAG 
AGATACTGTT TCAGGCCTTC TTATCAGTGT CCAAGCATGT TCTGTGATAC CCCCATGGTA 
CATCATATTC GGCGCTTTGT TCATGGCCAA GGCTGTGTGC AGATAATCCT GAAGGAGTTG 
GATTCTCCAG TACCTGGATA TCAGCATACA ATTCTTACAT ATTCCTGGTG TAGAATCTGC 
AAACAGGTAA CACCAGTTGT TGCTCTTTCC AATGAGTCCT GGTCTATGTC ATTTGCAAAA 
TACCTTGAAC TTAGGTTTTA TGGGCACCAG TATACTCGCA GAGCCAACGC TGAGCCCTGT 
GGTCACTCCA TCCATCATGA TTATCACCAG TATTTCTCCT ATAACCAGAT GGTGGCGTCT 
TTCAGTTATT CTCCCATTCG GCTTCTTGAA GTATGTGTTC CACTCCCCAA AATATTCATT 
AAGCGTCAGG CCCCATTAAA AGTGTCCCTT CTTCAGGATC TGAAGGACTT CTTTCAAAAA 
GTTTCACAGG TATATGTTGC CATTGATGAA AGACTTGCAT CTTTGAAAAC TGATACATTT 
AGTAAAACAA GAGAGGAAAA AATGGAAGAT ATTTTTGCAC AGAAAGAGAT GGAAGAAGGT 
GAGTTCAAGA ACTGGATTGA GAAGATGCAA GCAAGGCTCA TGTCTTCCTC TGTAGATACC 
CCTCAGCAAC TGCAGTCGGT CTTTGAGTCA CTCATTGCCA AGAAACAAAG TCTCTGTGAA 
GTGCTGCAAG CTTGGAATAA CAGGTTGCAG GACCTTTTCC AACAGGAAAA GGGTAGAAAG 
AGACCTTCAG TTCCTCCAAG TCCTGGAAGA CTGAGACAAG GGGAAGAAAG CAAGATAAGT 
GCGATGGATG CATCTCCACG GAATATTTCT CCAGGACTTC AGAATGGAGA AAAAGAGGAT 
CGCTTCTTAA CAACTTTGTC CAGCCAGAGC TCCACCAGTT CTACTCATCT CCAATTGCCT 
ACGCCACCTG AAGTCATGTC TGAACAGTCA GTGGGAGGGC CCCCTGAGCT AGATACAGCC 
AGCAGTTCCG AAGATGTGTT TGATGGGCAT TTGCTGGGAT CCACAGACAG CCAAGTGAAG 
GAAAAGTCAA CCATGAAAGC CATCTTTGCA AATTTGCTTC CAGGAAATAG CTATAATCCT 
ATTCCATTTC CTTTTGATCC AGATAAACAC TACTTAATGT ATGAACATGA ACGAGTGCCC 
ATTGCAGTCT GCGAGAAGGA ACCCAGCTCC ATCATTGCTT TTGCTCTCAG TTGTAAAGAA 
TACCGAAATG CCTTAGAGGA ATTGTCTAAA GCGACTCAGT GGAACAGTGC CGAAGAAGGG 
CTTCCAACAA ATAGTACTTC AGATAGCAGA CCAAAGAGTA GCAGCCCTAT CAGATTACCT 
GAAATGAGTG GAGGACAGAC AAATCGTACA ACAGAAACAG AACCACAACC AACCAAAAAG 
GCTTCTGGAA TGTTGTCCTT CTTCAGAGGG ACAGCAGGGA AAAGCCCCGA TCTCTCTTCC 
CAGAAGAGAG AGACCTTACG TGGAGCAGAT AGTGCTTACT ACCAGGTTGG GCAGACGGGC 
AAGGAGGGGA CCGAGAATCA AGGCGTTGAG CCTCAAGATG AAGTAGATGG AGGAGATACA 
CAAAAGAAGC AACTCATAAA TCCTCATGTG GAACTTCAAT TTTCAGATGC TAATGCCAAG 
TTTTACTGTC GGCTCTACTA TGCGGGAGAG TTTCATAAGA TGCGTGAAGT GATTCTGGAC 
AGCAGTGAAG AAGATTTCAT TCGTTCCCTC TCCCACTCAT CACCCTGGCA GGCCCGGGGA 
GGCAAATCAG GAGCTGCCTT CTATGCAACT GAGGATGATA GATTTATTTT GAAGCAAATG 
CCTCGTCTGG AAGTCCAGTC CTTCCTCGAC TTTGCACCAC ATTACTTCAA TTATATTACA 
AATGCTGTTC AACAAAAGAG GCCCACGGCG TTGGCCAAAA TTCTTGGAGT TTACAGAATT 
GGTTATAAGA ACTCTCAGAA CAACACTGAG AAGAAGTTAG ATCTCCTTGT CATGGAAAAT 
CTTTTCTACG GGAGAAAGAT GGCACAGGTT TTTGATTTGA AGGGCTCTCT TAGGAATCGG 
AATGTAAAAA CTGACACTGG AAAAGAGAGT TGTGATGTGG TCCTGCTAGA TGAAAATCTC 
CTAAAGATGG TTCGAGACAA CCCTCTATAT ATTCGTTCTC ATTCCAAAGC TGTGCTGAGA 
ACCTCGATCC ATAGTGACTC CCATTTCCTT TCTAGCCACC TCATTATAGA TTATTCTTTG 
CTGGTTGGGC GAGATGATAC TAGCAATGAG CTAGTAGTTG GAATTATAGA TTATATTCGA 
ACATTTACAT GGGACAAAAA GCTTGAGATG GTTGTGAAAT CAACAGGAAT TTTAGGTGGA 
CAAGGTAAAA TGCCAACAGT GGTGTCTCCG GAGTTGTACA GGACTAGGTT TTGTGAGGCA 
ATGGACAAGT ATTTCCTAAT GGTACCAGAC CACTGGACAG GCTTGGGTCT GAATTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PIKFYVE ( XM_017003572.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016859061.1 Homo sapiens phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X18, mRNA.

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CloneID	OHu77672
Clone ID Related Accession (Same CDS sequence)	XM_011510790.1 , XM_017003572.1 , XM_017003573.1 , XM_017003574.1
Accession Version	XM_017003572.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5340bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	1-phosphatidylinositol 3-phosphate 5-kinase isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTACCTT CATATGAGAC ATCTGTCAGT CCCCAGGCTA ACCGAACATA TGTTAGGACA 
GAGACCACTG AGGATGAACG CAAAATTCTT CTGGACAGTG TGCAGTTAAA AGACCTGTGG 
AAAAAAATCT GCCATCACAG CAGTGGAATG GAGTTTCAGG ATCACCGCTA CTGGTTGAGA 
ACGCATCCCA ACTGCATTGT AGGAAAGGAA TTAGTCAACT GGCTAATCCG AAATGGGCAT 
ATTGCCACAA GGGCACAAGC TATAGCAATT GGACAAGCAA TGGTTGATGG ACGTTGGCTG 
GATTGTGTTA GTCATCACGA CCAGCTTTTC AGAGATGAGT ATGCGCTGTA TAGACCACTG 
CAGAGTACAG AATTTTCTGA GACGCCTTCT CCCGACAGTG ACTCAGTGAA CTCCGTGGAA 
GGACACTCTG AGCCATCCTG GTTTAAAGAC ATAAAGTTTG ATGACAGTGA CACAGAACAG 
ATAGCTGAAG AAGGTGACGA TAATTTGGCT AATTCTGCCA GTCCTAGCAA GCGCACATCA 
GTCAGCAGTT TCCAGTCCAC AGTGGACAGT GACTCAGCCG CTTCTATCAG CCTGAACGTG 
GAGCTGGACA ACGTGAACTT CCATATCAAG AAGCCCTCCA AGTACCCACA TGTGCCCCCT 
CACCCTGCTG ACCAAAAAGA GTATTTGATT TCTGACACTG GAGGACAACA GCTCTCAATA 
AGTGACGCTT TCATCAAAGA ATCCTTATTT AATCGCCGAG TAGAGGAAAA ATCCAAAGAG 
CTGCCTTTCA CACCTTTGGG CTGGCATCAT AACAACCTGG AGCTCCTGAG GGAGGAGAAT 
GGGGAGAAAC AAGCCATGGA GAGGTTGCTT TCAGCTAATC ATAACCACAT GATGGCACTA 
CTCCAGCAGT TGCTCCATAG TGACTCACTG TCATCATCTT GGAGGGACAT CATCGTGTCA 
TTGGTCTGCC AGGTTGTTCA GACAGTCCGA CCTGATGTCA AGAACCAGGA TGATGACATG 
GATATCCGTC AGTTTGTCCA CATCAAAAAA ATCCCAGGTG GAAAGAAGTT TGATTCTGTG 
GTTGTCAATG GCTTTGTTTG TACCAAGAAC ATTGCACATA AAAAGATGAG TTCTTGTATT 
AAAAACCCCA AAATTCTTCT GTTGAAGTGT TCCATTGAGT ATCTCTACAG AGAAGAAACT 
AAGTTTACTT GCATTGATCC TATTGTGCTT CAGGAAAGGG AATTCTTGAA GAATTATGTC 
CAGCGAATAG TTGATGTTCG ACCCACCTTG GTTCTTGTTG AGAAAACAGT GTCTCGGATT 
GCCCAGGACA TGTTATTGGA ACATGGCATT ACTTTGGTCA TTAATGTAAA GTCACAAGTT 
TTGGAACGAA TCAGTCGAAT GACCCAAGGT GATTTAGTGA TGTCAATGGA CCAGCTGCTT 
ACGAAACCAC ACCTGGGCAC TTGTCACAAA TTTTATATGC AGATATTTCA GTTGCCTAAT 
GAACAAACCA AGACACTGAT GTTTTTTGAA GGTTGTCCAC AGCACCTAGG CTGTACAATC 
AAGCTAAGAG GAGGCTCTGA TTATGAGCTG GCTCGAGTTA AGGAGATCCT AATATTTATG 
ATCTGTGTTG CTTATCATTC TCAACTAGAA ATATCCTTTC TCATGGATGA ATTTGCTATG 
CCTCCCACAT TAATGCAAAA CCCTTCATTC CATTCCCTGA TTGAGGGACG AGGGCATGAG 
GGGGCTGTCC AAGAGCAGTA CGGTGGAGGT TCCATCCCCT GGGATCCTGA CATCCCTCCT 
GAGTCTCTGC CCTGTGATGA TAGCAGTTTG CTGGAATTGA GGATTGTGTT TGAGAAGGGT 
GAGCAGGAAA ATAAAAATCT TCCGCAGGCT GTTGCCTCTG TGAAGCATCA AGAACATAGC 
ACAACAGCTT GCCCGGCGGG TCTCCCTTGT GCTTTCTTTG CACCTGTACC GGAATCATTG 
TTGCCACTCC CTGTGGATGA CCAACAAGAT GCTTTAGGCA GCGAGCAGCC AGAGACTTTG 
CAGCAAACAG TTGTGCTGCA GGATCCCAAA AGCCAGATAA GAGCCTTTAG AGACCCTCTA 
CAGGATGACA CTGGATTATA TGTTACTGAG GAAGTCACCT CCTCTGAAGA TAAACGAAAG 
ACTTATTCTT TGGCCTTTAA GCAGGAATTA AAAGATGTGA TCCTCTGTAT CTCCCCAGTA 
ATCACATTCC GAGAACCCTT TCTTTTAACT GAAAAGGGGA TGAGATGCTC TACCCGAGAT 
TATTTTGCAG AGCAGGTTTA CTGGTCTCCT CTCCTCAATA AAGAATTCAA AGAAATGGAG 
AACAGGAGGA AGAAACAGCT GCTCAGGGAT CTCTCTGGAC TTCAGGGCAT GAATGGAAGT 
ATTCAGGCCA AGTCTATTCA AGTCTTACCC TCACATGAGC TAGTGAGCAC TAGAATTGCT 
GAGCATCTGG GCGATAGCCA GAGCTTGGGT AGAATGCTGG CCGATTATCG AGCCAGAGGA 
GGAAGAATTC AGCCCAAAAA TTCAGACCCT TTTGCTCATT CAAAGGATGC ATCAAGTACT 
TCAAGTGGCC AATCAGGAAG CAAAAATGAG GGTGATGAAG AGAGAGGGCT TATTCTGAGT 
GATGCTGTGT GGTCAACAAA GGTGGACTGT CTGAATCCCA TTAATCACCA GAGACTTTGT 
GTGCTCTTCA GCAGCTCTTC TGCCCAGTCC AGCAATGCTC CTAGTGCCTG TGTCAGTCCT 
TGGATTGTAA CAATGGAATT TTATGGAAAG AATGATCTTA CATTAGGAAT ATTTTTAGAG 
AGATACTGTT TCAGGCCTTC TTATCAGTGT CCAAGCATGT TCTGTGATAC CCCCATGGTA 
CATCATATTC GGCGCTTTGT TCATGGCCAA GGCTGTGTGC AGATAATCCT GAAGGAGTTG 
GATTCTCCAG TACCTGGATA TCAGCATACA ATTCTTACAT ATTCCTGGTG TAGAATCTGC 
AAACAGGTAA CACCAGTTGT TGCTCTTTCC AATGAGTCCT GGTCTATGTC ATTTGCAAAA 
TACCTTGAAC TTAGGTTTTA TGGGCACCAG TATACTCGCA GAGCCAACGC TGAGCCCTGT 
GGTCACTCCA TCCATCATGA TTATCACCAG TATTTCTCCT ATAACCAGAT GGTGGCGTCT 
TTCAGTTATT CTCCCATTCG GCTTCTTGAA GTATGTGTTC CACTCCCCAA AATATTCATT 
AAGCGTCAGG CCCCATTAAA AGTGTCCCTT CTTCAGGATC TGAAGGACTT CTTTCAAAAA 
GTTTCACAGG TATATGTTGC CATTGATGAA AGACTTGCAT CTTTGAAAAC TGATACATTT 
AGTAAAACAA GAGAGGAAAA AATGGAAGAT ATTTTTGCAC AGAAAGAGAT GGAAGAAGGT 
GAGTTCAAGA ACTGGATTGA GAAGATGCAA GCAAGGCTCA TGTCTTCCTC TGTAGATACC 
CCTCAGCAAC TGCAGTCGGT CTTTGAGTCA CTCATTGCCA AGAAACAAAG TCTCTGTGAA 
GTGCTGCAAG CTTGGAATAA CAGGTTGCAG GACCTTTTCC AACAGGAAAA GGGTAGAAAG 
AGACCTTCAG TTCCTCCAAG TCCTGGAAGA CTGAGACAAG GGGAAGAAAG CAAGATAAGT 
GCGATGGATG CATCTCCACG GAATATTTCT CCAGGACTTC AGAATGGAGA AAAAGAGGAT 
CGCTTCTTAA CAACTTTGTC CAGCCAGAGC TCCACCAGTT CTACTCATCT CCAATTGCCT 
ACGCCACCTG AAGTCATGTC TGAACAGTCA GTGGGAGGGC CCCCTGAGCT AGATACAGCC 
AGCAGTTCCG AAGATGTGTT TGATGGGCAT TTGCTGGGAT CCACAGACAG CCAAGTGAAG 
GAAAAGTCAA CCATGAAAGC CATCTTTGCA AATTTGCTTC CAGGAAATAG CTATAATCCT 
ATTCCATTTC CTTTTGATCC AGATAAACAC TACTTAATGT ATGAACATGA ACGAGTGCCC 
ATTGCAGTCT GCGAGAAGGA ACCCAGCTCC ATCATTGCTT TTGCTCTCAG TTGTAAAGAA 
TACCGAAATG CCTTAGAGGA ATTGTCTAAA GCGACTCAGT GGAACAGTGC CGAAGAAGGG 
CTTCCAACAA ATAGTACTTC AGATAGCAGA CCAAAGAGTA GCAGCCCTAT CAGATTACCT 
GAAATGAGTG GAGGACAGAC AAATCGTACA ACAGAAACAG AACCACAACC AACCAAAAAG 
GCTTCTGGAA TGTTGTCCTT CTTCAGAGGG ACAGCAGGGA AAAGCCCCGA TCTCTCTTCC 
CAGAAGAGAG AGACCTTACG TGGAGCAGAT AGTGCTTACT ACCAGGTTGG GCAGACGGGC 
AAGGAGGGGA CCGAGAATCA AGGCGTTGAG CCTCAAGATG AAGTAGATGG AGGAGATACA 
CAAAAGAAGC AACTCATAAA TCCTCATGTG GAACTTCAAT TTTCAGATGC TAATGCCAAG 
TTTTACTGTC GGCTCTACTA TGCGGGAGAG TTTCATAAGA TGCGTGAAGT GATTCTGGAC 
AGCAGTGAAG AAGATTTCAT TCGTTCCCTC TCCCACTCAT CACCCTGGCA GGCCCGGGGA 
GGCAAATCAG GAGCTGCCTT CTATGCAACT GAGGATGATA GATTTATTTT GAAGCAAATG 
CCTCGTCTGG AAGTCCAGTC CTTCCTCGAC TTTGCACCAC ATTACTTCAA TTATATTACA 
AATGCTGTTC AACAAAAGAG GCCCACGGCG TTGGCCAAAA TTCTTGGAGT TTACAGAATT 
GGTTATAAGA ACTCTCAGAA CAACACTGAG AAGAAGTTAG ATCTCCTTGT CATGGAAAAT 
CTTTTCTACG GGAGAAAGAT GGCACAGGTT TTTGATTTGA AGGGCTCTCT TAGGAATCGG 
AATGTAAAAA CTGACACTGG AAAAGAGAGT TGTGATGTGG TCCTGCTAGA TGAAAATCTC 
CTAAAGATGG TTCGAGACAA CCCTCTATAT ATTCGTTCTC ATTCCAAAGC TGTGCTGAGA 
ACCTCGATCC ATAGTGACTC CCATTTCCTT TCTAGCCACC TCATTATAGA TTATTCTTTG 
CTGGTTGGGC GAGATGATAC TAGCAATGAG CTAGTAGTTG GAATTATAGA TTATATTCGA 
ACATTTACAT GGGACAAAAA GCTTGAGATG GTTGTGAAAT CAACAGGAAT TTTAGGTGGA 
CAAGGTAAAA TGCCAACAGT GGTGTCTCCG GAGTTGTACA GGACTAGGTT TTGTGAGGCA 
ATGGACAAGT ATTTCCTAAT GGTACCAGAC CACTGGACAG GCTTGGGTCT GAATTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016859061.1
CDS	820..6159
Translation	MVPSYETSVSPQANRTYVRTETTEDERKILLDSVQLKDLWKKICHHSSGMEFQDHRYWLRTHPNCIVGKELVNWLIRNGHIATRAQAIAIGQAMVDGRWLDCVSHHDQLFRDEYALYRPLQSTEFSETPSPDSDSVNSVEGHSEPSWFKDIKFDDSDTEQIAEEGDDNLANSASPSKRTSVSSFQSTVDSDSAASISLNVELDNVNFHIKKPSKYPHVPPHPADQKEYLISDTGGQQLSISDAFIKESLFNRRVEEKSKELPFTPLGWHHNNLELLREENGEKQAMERLLSANHNHMMALLQQLLHSDSLSSSWRDIIVSLVCQVVQTVRPDVKNQDDDMDIRQFVHIKKIPGGKKFDSVVVNGFVCTKNIAHKKMSSCIKNPKILLLKCSIEYLYREETKFTCIDPIVLQEREFLKNYVQRIVDVRPTLVLVEKTVSRIAQDMLLEHGITLVINVKSQVLERISRMTQGDLVMSMDQLLTKPHLGTCHKFYMQIFQLPNEQTKTLMFFEGCPQHLGCTIKLRGGSDYELARVKEILIFMICVAYHSQLEISFLMDEFAMPPTLMQNPSFHSLIEGRGHEGAVQEQYGGGSIPWDPDIPPESLPCDDSSLLELRIVFEKGEQENKNLPQAVASVKHQEHSTTACPAGLPCAFFAPVPESLLPLPVDDQQDALGSEQPETLQQTVVLQDPKSQIRAFRDPLQDDTGLYVTEEVTSSEDKRKTYSLAFKQELKDVILCISPVITFREPFLLTEKGMRCSTRDYFAEQVYWSPLLNKEFKEMENRRKKQLLRDLSGLQGMNGSIQAKSIQVLPSHELVSTRIAEHLGDSQSLGRMLADYRARGGRIQPKNSDPFAHSKDASSTSSGQSGSKNEGDEERGLILSDAVWSTKVDCLNPINHQRLCVLFSSSSAQSSNAPSACVSPWIVTMEFYGKNDLTLGIFLERYCFRPSYQCPSMFCDTPMVHHIRRFVHGQGCVQIILKELDSPVPGYQHTILTYSWCRICKQVTPVVALSNESWSMSFAKYLELRFYGHQYTRRANAEPCGHSIHHDYHQYFSYNQMVASFSYSPIRLLEVCVPLPKIFIKRQAPLKVSLLQDLKDFFQKVSQVYVAIDERLASLKTDTFSKTREEKMEDIFAQKEMEEGEFKNWIEKMQARLMSSSVDTPQQLQSVFESLIAKKQSLCEVLQAWNNRLQDLFQQEKGRKRPSVPPSPGRLRQGEESKISAMDASPRNISPGLQNGEKEDRFLTTLSSQSSTSSTHLQLPTPPEVMSEQSVGGPPELDTASSSEDVFDGHLLGSTDSQVKEKSTMKAIFANLLPGNSYNPIPFPFDPDKHYLMYEHERVPIAVCEKEPSSIIAFALSCKEYRNALEELSKATQWNSAEEGLPTNSTSDSRPKSSSPIRLPEMSGGQTNRTTETEPQPTKKASGMLSFFRGTAGKSPDLSSQKRETLRGADSAYYQVGQTGKEGTENQGVEPQDEVDGGDTQKKQLINPHVELQFSDANAKFYCRLYYAGEFHKMREVILDSSEEDFIRSLSHSSPWQARGGKSGAAFYATEDDRFILKQMPRLEVQSFLDFAPHYFNYITNAVQQKRPTALAKILGVYRIGYKNSQNNTEKKLDLLVMENLFYGRKMAQVFDLKGSLRNRNVKTDTGKESCDVVLLDENLLKMVRDNPLYIRSHSKAVLRTSIHSDSHFLSSHLIIDYSLLVGRDDTSNELVVGIIDYIRTFTWDKKLEMVVKSTGILGGQGKMPTVVSPELYRTRFCEAMDKYFLMVPDHWTGLGLNC

Target ORF information:

RefSeq Version	XM_017003572.1
Organism	Homo sapiens(human)
Definition	Homo sapiens phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017003572.1

ORF Insert Sequence:

ATGGTACCTT CATATGAGAC ATCTGTCAGT CCCCAGGCTA ACCGAACATA TGTTAGGACA 
GAGACCACTG AGGATGAACG CAAAATTCTT CTGGACAGTG TGCAGTTAAA AGACCTGTGG 
AAAAAAATCT GCCATCACAG CAGTGGAATG GAGTTTCAGG ATCACCGCTA CTGGTTGAGA 
ACGCATCCCA ACTGCATTGT AGGAAAGGAA TTAGTCAACT GGCTAATCCG AAATGGGCAT 
ATTGCCACAA GGGCACAAGC TATAGCAATT GGACAAGCAA TGGTTGATGG ACGTTGGCTG 
GATTGTGTTA GTCATCACGA CCAGCTTTTC AGAGATGAGT ATGCGCTGTA TAGACCACTG 
CAGAGTACAG AATTTTCTGA GACGCCTTCT CCCGACAGTG ACTCAGTGAA CTCCGTGGAA 
GGACACTCTG AGCCATCCTG GTTTAAAGAC ATAAAGTTTG ATGACAGTGA CACAGAACAG 
ATAGCTGAAG AAGGTGACGA TAATTTGGCT AATTCTGCCA GTCCTAGCAA GCGCACATCA 
GTCAGCAGTT TCCAGTCCAC AGTGGACAGT GACTCAGCCG CTTCTATCAG CCTGAACGTG 
GAGCTGGACA ACGTGAACTT CCATATCAAG AAGCCCTCCA AGTACCCACA TGTGCCCCCT 
CACCCTGCTG ACCAAAAAGA GTATTTGATT TCTGACACTG GAGGACAACA GCTCTCAATA 
AGTGACGCTT TCATCAAAGA ATCCTTATTT AATCGCCGAG TAGAGGAAAA ATCCAAAGAG 
CTGCCTTTCA CACCTTTGGG CTGGCATCAT AACAACCTGG AGCTCCTGAG GGAGGAGAAT 
GGGGAGAAAC AAGCCATGGA GAGGTTGCTT TCAGCTAATC ATAACCACAT GATGGCACTA 
CTCCAGCAGT TGCTCCATAG TGACTCACTG TCATCATCTT GGAGGGACAT CATCGTGTCA 
TTGGTCTGCC AGGTTGTTCA GACAGTCCGA CCTGATGTCA AGAACCAGGA TGATGACATG 
GATATCCGTC AGTTTGTCCA CATCAAAAAA ATCCCAGGTG GAAAGAAGTT TGATTCTGTG 
GTTGTCAATG GCTTTGTTTG TACCAAGAAC ATTGCACATA AAAAGATGAG TTCTTGTATT 
AAAAACCCCA AAATTCTTCT GTTGAAGTGT TCCATTGAGT ATCTCTACAG AGAAGAAACT 
AAGTTTACTT GCATTGATCC TATTGTGCTT CAGGAAAGGG AATTCTTGAA GAATTATGTC 
CAGCGAATAG TTGATGTTCG ACCCACCTTG GTTCTTGTTG AGAAAACAGT GTCTCGGATT 
GCCCAGGACA TGTTATTGGA ACATGGCATT ACTTTGGTCA TTAATGTAAA GTCACAAGTT 
TTGGAACGAA TCAGTCGAAT GACCCAAGGT GATTTAGTGA TGTCAATGGA CCAGCTGCTT 
ACGAAACCAC ACCTGGGCAC TTGTCACAAA TTTTATATGC AGATATTTCA GTTGCCTAAT 
GAACAAACCA AGACACTGAT GTTTTTTGAA GGTTGTCCAC AGCACCTAGG CTGTACAATC 
AAGCTAAGAG GAGGCTCTGA TTATGAGCTG GCTCGAGTTA AGGAGATCCT AATATTTATG 
ATCTGTGTTG CTTATCATTC TCAACTAGAA ATATCCTTTC TCATGGATGA ATTTGCTATG 
CCTCCCACAT TAATGCAAAA CCCTTCATTC CATTCCCTGA TTGAGGGACG AGGGCATGAG 
GGGGCTGTCC AAGAGCAGTA CGGTGGAGGT TCCATCCCCT GGGATCCTGA CATCCCTCCT 
GAGTCTCTGC CCTGTGATGA TAGCAGTTTG CTGGAATTGA GGATTGTGTT TGAGAAGGGT 
GAGCAGGAAA ATAAAAATCT TCCGCAGGCT GTTGCCTCTG TGAAGCATCA AGAACATAGC 
ACAACAGCTT GCCCGGCGGG TCTCCCTTGT GCTTTCTTTG CACCTGTACC GGAATCATTG 
TTGCCACTCC CTGTGGATGA CCAACAAGAT GCTTTAGGCA GCGAGCAGCC AGAGACTTTG 
CAGCAAACAG TTGTGCTGCA GGATCCCAAA AGCCAGATAA GAGCCTTTAG AGACCCTCTA 
CAGGATGACA CTGGATTATA TGTTACTGAG GAAGTCACCT CCTCTGAAGA TAAACGAAAG 
ACTTATTCTT TGGCCTTTAA GCAGGAATTA AAAGATGTGA TCCTCTGTAT CTCCCCAGTA 
ATCACATTCC GAGAACCCTT TCTTTTAACT GAAAAGGGGA TGAGATGCTC TACCCGAGAT 
TATTTTGCAG AGCAGGTTTA CTGGTCTCCT CTCCTCAATA AAGAATTCAA AGAAATGGAG 
AACAGGAGGA AGAAACAGCT GCTCAGGGAT CTCTCTGGAC TTCAGGGCAT GAATGGAAGT 
ATTCAGGCCA AGTCTATTCA AGTCTTACCC TCACATGAGC TAGTGAGCAC TAGAATTGCT 
GAGCATCTGG GCGATAGCCA GAGCTTGGGT AGAATGCTGG CCGATTATCG AGCCAGAGGA 
GGAAGAATTC AGCCCAAAAA TTCAGACCCT TTTGCTCATT CAAAGGATGC ATCAAGTACT 
TCAAGTGGCC AATCAGGAAG CAAAAATGAG GGTGATGAAG AGAGAGGGCT TATTCTGAGT 
GATGCTGTGT GGTCAACAAA GGTGGACTGT CTGAATCCCA TTAATCACCA GAGACTTTGT 
GTGCTCTTCA GCAGCTCTTC TGCCCAGTCC AGCAATGCTC CTAGTGCCTG TGTCAGTCCT 
TGGATTGTAA CAATGGAATT TTATGGAAAG AATGATCTTA CATTAGGAAT ATTTTTAGAG 
AGATACTGTT TCAGGCCTTC TTATCAGTGT CCAAGCATGT TCTGTGATAC CCCCATGGTA 
CATCATATTC GGCGCTTTGT TCATGGCCAA GGCTGTGTGC AGATAATCCT GAAGGAGTTG 
GATTCTCCAG TACCTGGATA TCAGCATACA ATTCTTACAT ATTCCTGGTG TAGAATCTGC 
AAACAGGTAA CACCAGTTGT TGCTCTTTCC AATGAGTCCT GGTCTATGTC ATTTGCAAAA 
TACCTTGAAC TTAGGTTTTA TGGGCACCAG TATACTCGCA GAGCCAACGC TGAGCCCTGT 
GGTCACTCCA TCCATCATGA TTATCACCAG TATTTCTCCT ATAACCAGAT GGTGGCGTCT 
TTCAGTTATT CTCCCATTCG GCTTCTTGAA GTATGTGTTC CACTCCCCAA AATATTCATT 
AAGCGTCAGG CCCCATTAAA AGTGTCCCTT CTTCAGGATC TGAAGGACTT CTTTCAAAAA 
GTTTCACAGG TATATGTTGC CATTGATGAA AGACTTGCAT CTTTGAAAAC TGATACATTT 
AGTAAAACAA GAGAGGAAAA AATGGAAGAT ATTTTTGCAC AGAAAGAGAT GGAAGAAGGT 
GAGTTCAAGA ACTGGATTGA GAAGATGCAA GCAAGGCTCA TGTCTTCCTC TGTAGATACC 
CCTCAGCAAC TGCAGTCGGT CTTTGAGTCA CTCATTGCCA AGAAACAAAG TCTCTGTGAA 
GTGCTGCAAG CTTGGAATAA CAGGTTGCAG GACCTTTTCC AACAGGAAAA GGGTAGAAAG 
AGACCTTCAG TTCCTCCAAG TCCTGGAAGA CTGAGACAAG GGGAAGAAAG CAAGATAAGT 
GCGATGGATG CATCTCCACG GAATATTTCT CCAGGACTTC AGAATGGAGA AAAAGAGGAT 
CGCTTCTTAA CAACTTTGTC CAGCCAGAGC TCCACCAGTT CTACTCATCT CCAATTGCCT 
ACGCCACCTG AAGTCATGTC TGAACAGTCA GTGGGAGGGC CCCCTGAGCT AGATACAGCC 
AGCAGTTCCG AAGATGTGTT TGATGGGCAT TTGCTGGGAT CCACAGACAG CCAAGTGAAG 
GAAAAGTCAA CCATGAAAGC CATCTTTGCA AATTTGCTTC CAGGAAATAG CTATAATCCT 
ATTCCATTTC CTTTTGATCC AGATAAACAC TACTTAATGT ATGAACATGA ACGAGTGCCC 
ATTGCAGTCT GCGAGAAGGA ACCCAGCTCC ATCATTGCTT TTGCTCTCAG TTGTAAAGAA 
TACCGAAATG CCTTAGAGGA ATTGTCTAAA GCGACTCAGT GGAACAGTGC CGAAGAAGGG 
CTTCCAACAA ATAGTACTTC AGATAGCAGA CCAAAGAGTA GCAGCCCTAT CAGATTACCT 
GAAATGAGTG GAGGACAGAC AAATCGTACA ACAGAAACAG AACCACAACC AACCAAAAAG 
GCTTCTGGAA TGTTGTCCTT CTTCAGAGGG ACAGCAGGGA AAAGCCCCGA TCTCTCTTCC 
CAGAAGAGAG AGACCTTACG TGGAGCAGAT AGTGCTTACT ACCAGGTTGG GCAGACGGGC 
AAGGAGGGGA CCGAGAATCA AGGCGTTGAG CCTCAAGATG AAGTAGATGG AGGAGATACA 
CAAAAGAAGC AACTCATAAA TCCTCATGTG GAACTTCAAT TTTCAGATGC TAATGCCAAG 
TTTTACTGTC GGCTCTACTA TGCGGGAGAG TTTCATAAGA TGCGTGAAGT GATTCTGGAC 
AGCAGTGAAG AAGATTTCAT TCGTTCCCTC TCCCACTCAT CACCCTGGCA GGCCCGGGGA 
GGCAAATCAG GAGCTGCCTT CTATGCAACT GAGGATGATA GATTTATTTT GAAGCAAATG 
CCTCGTCTGG AAGTCCAGTC CTTCCTCGAC TTTGCACCAC ATTACTTCAA TTATATTACA 
AATGCTGTTC AACAAAAGAG GCCCACGGCG TTGGCCAAAA TTCTTGGAGT TTACAGAATT 
GGTTATAAGA ACTCTCAGAA CAACACTGAG AAGAAGTTAG ATCTCCTTGT CATGGAAAAT 
CTTTTCTACG GGAGAAAGAT GGCACAGGTT TTTGATTTGA AGGGCTCTCT TAGGAATCGG 
AATGTAAAAA CTGACACTGG AAAAGAGAGT TGTGATGTGG TCCTGCTAGA TGAAAATCTC 
CTAAAGATGG TTCGAGACAA CCCTCTATAT ATTCGTTCTC ATTCCAAAGC TGTGCTGAGA 
ACCTCGATCC ATAGTGACTC CCATTTCCTT TCTAGCCACC TCATTATAGA TTATTCTTTG 
CTGGTTGGGC GAGATGATAC TAGCAATGAG CTAGTAGTTG GAATTATAGA TTATATTCGA 
ACATTTACAT GGGACAAAAA GCTTGAGATG GTTGTGAAAT CAACAGGAAT TTTAGGTGGA 
CAAGGTAAAA TGCCAACAGT GGTGTCTCCG GAGTTGTACA GGACTAGGTT TTGTGAGGCA 
ATGGACAAGT ATTTCCTAAT GGTACCAGAC CACTGGACAG GCTTGGGTCT GAATTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PIKFYVE ( XM_017003573.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016859062.1 Homo sapiens phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X19, mRNA.

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CloneID	OHu77672
Clone ID Related Accession (Same CDS sequence)	XM_011510790.1 , XM_017003572.1 , XM_017003573.1 , XM_017003574.1
Accession Version	XM_017003573.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5340bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	1-phosphatidylinositol 3-phosphate 5-kinase isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTACCTT CATATGAGAC ATCTGTCAGT CCCCAGGCTA ACCGAACATA TGTTAGGACA 
GAGACCACTG AGGATGAACG CAAAATTCTT CTGGACAGTG TGCAGTTAAA AGACCTGTGG 
AAAAAAATCT GCCATCACAG CAGTGGAATG GAGTTTCAGG ATCACCGCTA CTGGTTGAGA 
ACGCATCCCA ACTGCATTGT AGGAAAGGAA TTAGTCAACT GGCTAATCCG AAATGGGCAT 
ATTGCCACAA GGGCACAAGC TATAGCAATT GGACAAGCAA TGGTTGATGG ACGTTGGCTG 
GATTGTGTTA GTCATCACGA CCAGCTTTTC AGAGATGAGT ATGCGCTGTA TAGACCACTG 
CAGAGTACAG AATTTTCTGA GACGCCTTCT CCCGACAGTG ACTCAGTGAA CTCCGTGGAA 
GGACACTCTG AGCCATCCTG GTTTAAAGAC ATAAAGTTTG ATGACAGTGA CACAGAACAG 
ATAGCTGAAG AAGGTGACGA TAATTTGGCT AATTCTGCCA GTCCTAGCAA GCGCACATCA 
GTCAGCAGTT TCCAGTCCAC AGTGGACAGT GACTCAGCCG CTTCTATCAG CCTGAACGTG 
GAGCTGGACA ACGTGAACTT CCATATCAAG AAGCCCTCCA AGTACCCACA TGTGCCCCCT 
CACCCTGCTG ACCAAAAAGA GTATTTGATT TCTGACACTG GAGGACAACA GCTCTCAATA 
AGTGACGCTT TCATCAAAGA ATCCTTATTT AATCGCCGAG TAGAGGAAAA ATCCAAAGAG 
CTGCCTTTCA CACCTTTGGG CTGGCATCAT AACAACCTGG AGCTCCTGAG GGAGGAGAAT 
GGGGAGAAAC AAGCCATGGA GAGGTTGCTT TCAGCTAATC ATAACCACAT GATGGCACTA 
CTCCAGCAGT TGCTCCATAG TGACTCACTG TCATCATCTT GGAGGGACAT CATCGTGTCA 
TTGGTCTGCC AGGTTGTTCA GACAGTCCGA CCTGATGTCA AGAACCAGGA TGATGACATG 
GATATCCGTC AGTTTGTCCA CATCAAAAAA ATCCCAGGTG GAAAGAAGTT TGATTCTGTG 
GTTGTCAATG GCTTTGTTTG TACCAAGAAC ATTGCACATA AAAAGATGAG TTCTTGTATT 
AAAAACCCCA AAATTCTTCT GTTGAAGTGT TCCATTGAGT ATCTCTACAG AGAAGAAACT 
AAGTTTACTT GCATTGATCC TATTGTGCTT CAGGAAAGGG AATTCTTGAA GAATTATGTC 
CAGCGAATAG TTGATGTTCG ACCCACCTTG GTTCTTGTTG AGAAAACAGT GTCTCGGATT 
GCCCAGGACA TGTTATTGGA ACATGGCATT ACTTTGGTCA TTAATGTAAA GTCACAAGTT 
TTGGAACGAA TCAGTCGAAT GACCCAAGGT GATTTAGTGA TGTCAATGGA CCAGCTGCTT 
ACGAAACCAC ACCTGGGCAC TTGTCACAAA TTTTATATGC AGATATTTCA GTTGCCTAAT 
GAACAAACCA AGACACTGAT GTTTTTTGAA GGTTGTCCAC AGCACCTAGG CTGTACAATC 
AAGCTAAGAG GAGGCTCTGA TTATGAGCTG GCTCGAGTTA AGGAGATCCT AATATTTATG 
ATCTGTGTTG CTTATCATTC TCAACTAGAA ATATCCTTTC TCATGGATGA ATTTGCTATG 
CCTCCCACAT TAATGCAAAA CCCTTCATTC CATTCCCTGA TTGAGGGACG AGGGCATGAG 
GGGGCTGTCC AAGAGCAGTA CGGTGGAGGT TCCATCCCCT GGGATCCTGA CATCCCTCCT 
GAGTCTCTGC CCTGTGATGA TAGCAGTTTG CTGGAATTGA GGATTGTGTT TGAGAAGGGT 
GAGCAGGAAA ATAAAAATCT TCCGCAGGCT GTTGCCTCTG TGAAGCATCA AGAACATAGC 
ACAACAGCTT GCCCGGCGGG TCTCCCTTGT GCTTTCTTTG CACCTGTACC GGAATCATTG 
TTGCCACTCC CTGTGGATGA CCAACAAGAT GCTTTAGGCA GCGAGCAGCC AGAGACTTTG 
CAGCAAACAG TTGTGCTGCA GGATCCCAAA AGCCAGATAA GAGCCTTTAG AGACCCTCTA 
CAGGATGACA CTGGATTATA TGTTACTGAG GAAGTCACCT CCTCTGAAGA TAAACGAAAG 
ACTTATTCTT TGGCCTTTAA GCAGGAATTA AAAGATGTGA TCCTCTGTAT CTCCCCAGTA 
ATCACATTCC GAGAACCCTT TCTTTTAACT GAAAAGGGGA TGAGATGCTC TACCCGAGAT 
TATTTTGCAG AGCAGGTTTA CTGGTCTCCT CTCCTCAATA AAGAATTCAA AGAAATGGAG 
AACAGGAGGA AGAAACAGCT GCTCAGGGAT CTCTCTGGAC TTCAGGGCAT GAATGGAAGT 
ATTCAGGCCA AGTCTATTCA AGTCTTACCC TCACATGAGC TAGTGAGCAC TAGAATTGCT 
GAGCATCTGG GCGATAGCCA GAGCTTGGGT AGAATGCTGG CCGATTATCG AGCCAGAGGA 
GGAAGAATTC AGCCCAAAAA TTCAGACCCT TTTGCTCATT CAAAGGATGC ATCAAGTACT 
TCAAGTGGCC AATCAGGAAG CAAAAATGAG GGTGATGAAG AGAGAGGGCT TATTCTGAGT 
GATGCTGTGT GGTCAACAAA GGTGGACTGT CTGAATCCCA TTAATCACCA GAGACTTTGT 
GTGCTCTTCA GCAGCTCTTC TGCCCAGTCC AGCAATGCTC CTAGTGCCTG TGTCAGTCCT 
TGGATTGTAA CAATGGAATT TTATGGAAAG AATGATCTTA CATTAGGAAT ATTTTTAGAG 
AGATACTGTT TCAGGCCTTC TTATCAGTGT CCAAGCATGT TCTGTGATAC CCCCATGGTA 
CATCATATTC GGCGCTTTGT TCATGGCCAA GGCTGTGTGC AGATAATCCT GAAGGAGTTG 
GATTCTCCAG TACCTGGATA TCAGCATACA ATTCTTACAT ATTCCTGGTG TAGAATCTGC 
AAACAGGTAA CACCAGTTGT TGCTCTTTCC AATGAGTCCT GGTCTATGTC ATTTGCAAAA 
TACCTTGAAC TTAGGTTTTA TGGGCACCAG TATACTCGCA GAGCCAACGC TGAGCCCTGT 
GGTCACTCCA TCCATCATGA TTATCACCAG TATTTCTCCT ATAACCAGAT GGTGGCGTCT 
TTCAGTTATT CTCCCATTCG GCTTCTTGAA GTATGTGTTC CACTCCCCAA AATATTCATT 
AAGCGTCAGG CCCCATTAAA AGTGTCCCTT CTTCAGGATC TGAAGGACTT CTTTCAAAAA 
GTTTCACAGG TATATGTTGC CATTGATGAA AGACTTGCAT CTTTGAAAAC TGATACATTT 
AGTAAAACAA GAGAGGAAAA AATGGAAGAT ATTTTTGCAC AGAAAGAGAT GGAAGAAGGT 
GAGTTCAAGA ACTGGATTGA GAAGATGCAA GCAAGGCTCA TGTCTTCCTC TGTAGATACC 
CCTCAGCAAC TGCAGTCGGT CTTTGAGTCA CTCATTGCCA AGAAACAAAG TCTCTGTGAA 
GTGCTGCAAG CTTGGAATAA CAGGTTGCAG GACCTTTTCC AACAGGAAAA GGGTAGAAAG 
AGACCTTCAG TTCCTCCAAG TCCTGGAAGA CTGAGACAAG GGGAAGAAAG CAAGATAAGT 
GCGATGGATG CATCTCCACG GAATATTTCT CCAGGACTTC AGAATGGAGA AAAAGAGGAT 
CGCTTCTTAA CAACTTTGTC CAGCCAGAGC TCCACCAGTT CTACTCATCT CCAATTGCCT 
ACGCCACCTG AAGTCATGTC TGAACAGTCA GTGGGAGGGC CCCCTGAGCT AGATACAGCC 
AGCAGTTCCG AAGATGTGTT TGATGGGCAT TTGCTGGGAT CCACAGACAG CCAAGTGAAG 
GAAAAGTCAA CCATGAAAGC CATCTTTGCA AATTTGCTTC CAGGAAATAG CTATAATCCT 
ATTCCATTTC CTTTTGATCC AGATAAACAC TACTTAATGT ATGAACATGA ACGAGTGCCC 
ATTGCAGTCT GCGAGAAGGA ACCCAGCTCC ATCATTGCTT TTGCTCTCAG TTGTAAAGAA 
TACCGAAATG CCTTAGAGGA ATTGTCTAAA GCGACTCAGT GGAACAGTGC CGAAGAAGGG 
CTTCCAACAA ATAGTACTTC AGATAGCAGA CCAAAGAGTA GCAGCCCTAT CAGATTACCT 
GAAATGAGTG GAGGACAGAC AAATCGTACA ACAGAAACAG AACCACAACC AACCAAAAAG 
GCTTCTGGAA TGTTGTCCTT CTTCAGAGGG ACAGCAGGGA AAAGCCCCGA TCTCTCTTCC 
CAGAAGAGAG AGACCTTACG TGGAGCAGAT AGTGCTTACT ACCAGGTTGG GCAGACGGGC 
AAGGAGGGGA CCGAGAATCA AGGCGTTGAG CCTCAAGATG AAGTAGATGG AGGAGATACA 
CAAAAGAAGC AACTCATAAA TCCTCATGTG GAACTTCAAT TTTCAGATGC TAATGCCAAG 
TTTTACTGTC GGCTCTACTA TGCGGGAGAG TTTCATAAGA TGCGTGAAGT GATTCTGGAC 
AGCAGTGAAG AAGATTTCAT TCGTTCCCTC TCCCACTCAT CACCCTGGCA GGCCCGGGGA 
GGCAAATCAG GAGCTGCCTT CTATGCAACT GAGGATGATA GATTTATTTT GAAGCAAATG 
CCTCGTCTGG AAGTCCAGTC CTTCCTCGAC TTTGCACCAC ATTACTTCAA TTATATTACA 
AATGCTGTTC AACAAAAGAG GCCCACGGCG TTGGCCAAAA TTCTTGGAGT TTACAGAATT 
GGTTATAAGA ACTCTCAGAA CAACACTGAG AAGAAGTTAG ATCTCCTTGT CATGGAAAAT 
CTTTTCTACG GGAGAAAGAT GGCACAGGTT TTTGATTTGA AGGGCTCTCT TAGGAATCGG 
AATGTAAAAA CTGACACTGG AAAAGAGAGT TGTGATGTGG TCCTGCTAGA TGAAAATCTC 
CTAAAGATGG TTCGAGACAA CCCTCTATAT ATTCGTTCTC ATTCCAAAGC TGTGCTGAGA 
ACCTCGATCC ATAGTGACTC CCATTTCCTT TCTAGCCACC TCATTATAGA TTATTCTTTG 
CTGGTTGGGC GAGATGATAC TAGCAATGAG CTAGTAGTTG GAATTATAGA TTATATTCGA 
ACATTTACAT GGGACAAAAA GCTTGAGATG GTTGTGAAAT CAACAGGAAT TTTAGGTGGA 
CAAGGTAAAA TGCCAACAGT GGTGTCTCCG GAGTTGTACA GGACTAGGTT TTGTGAGGCA 
ATGGACAAGT ATTTCCTAAT GGTACCAGAC CACTGGACAG GCTTGGGTCT GAATTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016859062.1
CDS	528..5867
Translation	MVPSYETSVSPQANRTYVRTETTEDERKILLDSVQLKDLWKKICHHSSGMEFQDHRYWLRTHPNCIVGKELVNWLIRNGHIATRAQAIAIGQAMVDGRWLDCVSHHDQLFRDEYALYRPLQSTEFSETPSPDSDSVNSVEGHSEPSWFKDIKFDDSDTEQIAEEGDDNLANSASPSKRTSVSSFQSTVDSDSAASISLNVELDNVNFHIKKPSKYPHVPPHPADQKEYLISDTGGQQLSISDAFIKESLFNRRVEEKSKELPFTPLGWHHNNLELLREENGEKQAMERLLSANHNHMMALLQQLLHSDSLSSSWRDIIVSLVCQVVQTVRPDVKNQDDDMDIRQFVHIKKIPGGKKFDSVVVNGFVCTKNIAHKKMSSCIKNPKILLLKCSIEYLYREETKFTCIDPIVLQEREFLKNYVQRIVDVRPTLVLVEKTVSRIAQDMLLEHGITLVINVKSQVLERISRMTQGDLVMSMDQLLTKPHLGTCHKFYMQIFQLPNEQTKTLMFFEGCPQHLGCTIKLRGGSDYELARVKEILIFMICVAYHSQLEISFLMDEFAMPPTLMQNPSFHSLIEGRGHEGAVQEQYGGGSIPWDPDIPPESLPCDDSSLLELRIVFEKGEQENKNLPQAVASVKHQEHSTTACPAGLPCAFFAPVPESLLPLPVDDQQDALGSEQPETLQQTVVLQDPKSQIRAFRDPLQDDTGLYVTEEVTSSEDKRKTYSLAFKQELKDVILCISPVITFREPFLLTEKGMRCSTRDYFAEQVYWSPLLNKEFKEMENRRKKQLLRDLSGLQGMNGSIQAKSIQVLPSHELVSTRIAEHLGDSQSLGRMLADYRARGGRIQPKNSDPFAHSKDASSTSSGQSGSKNEGDEERGLILSDAVWSTKVDCLNPINHQRLCVLFSSSSAQSSNAPSACVSPWIVTMEFYGKNDLTLGIFLERYCFRPSYQCPSMFCDTPMVHHIRRFVHGQGCVQIILKELDSPVPGYQHTILTYSWCRICKQVTPVVALSNESWSMSFAKYLELRFYGHQYTRRANAEPCGHSIHHDYHQYFSYNQMVASFSYSPIRLLEVCVPLPKIFIKRQAPLKVSLLQDLKDFFQKVSQVYVAIDERLASLKTDTFSKTREEKMEDIFAQKEMEEGEFKNWIEKMQARLMSSSVDTPQQLQSVFESLIAKKQSLCEVLQAWNNRLQDLFQQEKGRKRPSVPPSPGRLRQGEESKISAMDASPRNISPGLQNGEKEDRFLTTLSSQSSTSSTHLQLPTPPEVMSEQSVGGPPELDTASSSEDVFDGHLLGSTDSQVKEKSTMKAIFANLLPGNSYNPIPFPFDPDKHYLMYEHERVPIAVCEKEPSSIIAFALSCKEYRNALEELSKATQWNSAEEGLPTNSTSDSRPKSSSPIRLPEMSGGQTNRTTETEPQPTKKASGMLSFFRGTAGKSPDLSSQKRETLRGADSAYYQVGQTGKEGTENQGVEPQDEVDGGDTQKKQLINPHVELQFSDANAKFYCRLYYAGEFHKMREVILDSSEEDFIRSLSHSSPWQARGGKSGAAFYATEDDRFILKQMPRLEVQSFLDFAPHYFNYITNAVQQKRPTALAKILGVYRIGYKNSQNNTEKKLDLLVMENLFYGRKMAQVFDLKGSLRNRNVKTDTGKESCDVVLLDENLLKMVRDNPLYIRSHSKAVLRTSIHSDSHFLSSHLIIDYSLLVGRDDTSNELVVGIIDYIRTFTWDKKLEMVVKSTGILGGQGKMPTVVSPELYRTRFCEAMDKYFLMVPDHWTGLGLNC

Target ORF information:

RefSeq Version	XM_017003573.1
Organism	Homo sapiens(human)
Definition	Homo sapiens phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X19, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017003573.1

ORF Insert Sequence:

ATGGTACCTT CATATGAGAC ATCTGTCAGT CCCCAGGCTA ACCGAACATA TGTTAGGACA 
GAGACCACTG AGGATGAACG CAAAATTCTT CTGGACAGTG TGCAGTTAAA AGACCTGTGG 
AAAAAAATCT GCCATCACAG CAGTGGAATG GAGTTTCAGG ATCACCGCTA CTGGTTGAGA 
ACGCATCCCA ACTGCATTGT AGGAAAGGAA TTAGTCAACT GGCTAATCCG AAATGGGCAT 
ATTGCCACAA GGGCACAAGC TATAGCAATT GGACAAGCAA TGGTTGATGG ACGTTGGCTG 
GATTGTGTTA GTCATCACGA CCAGCTTTTC AGAGATGAGT ATGCGCTGTA TAGACCACTG 
CAGAGTACAG AATTTTCTGA GACGCCTTCT CCCGACAGTG ACTCAGTGAA CTCCGTGGAA 
GGACACTCTG AGCCATCCTG GTTTAAAGAC ATAAAGTTTG ATGACAGTGA CACAGAACAG 
ATAGCTGAAG AAGGTGACGA TAATTTGGCT AATTCTGCCA GTCCTAGCAA GCGCACATCA 
GTCAGCAGTT TCCAGTCCAC AGTGGACAGT GACTCAGCCG CTTCTATCAG CCTGAACGTG 
GAGCTGGACA ACGTGAACTT CCATATCAAG AAGCCCTCCA AGTACCCACA TGTGCCCCCT 
CACCCTGCTG ACCAAAAAGA GTATTTGATT TCTGACACTG GAGGACAACA GCTCTCAATA 
AGTGACGCTT TCATCAAAGA ATCCTTATTT AATCGCCGAG TAGAGGAAAA ATCCAAAGAG 
CTGCCTTTCA CACCTTTGGG CTGGCATCAT AACAACCTGG AGCTCCTGAG GGAGGAGAAT 
GGGGAGAAAC AAGCCATGGA GAGGTTGCTT TCAGCTAATC ATAACCACAT GATGGCACTA 
CTCCAGCAGT TGCTCCATAG TGACTCACTG TCATCATCTT GGAGGGACAT CATCGTGTCA 
TTGGTCTGCC AGGTTGTTCA GACAGTCCGA CCTGATGTCA AGAACCAGGA TGATGACATG 
GATATCCGTC AGTTTGTCCA CATCAAAAAA ATCCCAGGTG GAAAGAAGTT TGATTCTGTG 
GTTGTCAATG GCTTTGTTTG TACCAAGAAC ATTGCACATA AAAAGATGAG TTCTTGTATT 
AAAAACCCCA AAATTCTTCT GTTGAAGTGT TCCATTGAGT ATCTCTACAG AGAAGAAACT 
AAGTTTACTT GCATTGATCC TATTGTGCTT CAGGAAAGGG AATTCTTGAA GAATTATGTC 
CAGCGAATAG TTGATGTTCG ACCCACCTTG GTTCTTGTTG AGAAAACAGT GTCTCGGATT 
GCCCAGGACA TGTTATTGGA ACATGGCATT ACTTTGGTCA TTAATGTAAA GTCACAAGTT 
TTGGAACGAA TCAGTCGAAT GACCCAAGGT GATTTAGTGA TGTCAATGGA CCAGCTGCTT 
ACGAAACCAC ACCTGGGCAC TTGTCACAAA TTTTATATGC AGATATTTCA GTTGCCTAAT 
GAACAAACCA AGACACTGAT GTTTTTTGAA GGTTGTCCAC AGCACCTAGG CTGTACAATC 
AAGCTAAGAG GAGGCTCTGA TTATGAGCTG GCTCGAGTTA AGGAGATCCT AATATTTATG 
ATCTGTGTTG CTTATCATTC TCAACTAGAA ATATCCTTTC TCATGGATGA ATTTGCTATG 
CCTCCCACAT TAATGCAAAA CCCTTCATTC CATTCCCTGA TTGAGGGACG AGGGCATGAG 
GGGGCTGTCC AAGAGCAGTA CGGTGGAGGT TCCATCCCCT GGGATCCTGA CATCCCTCCT 
GAGTCTCTGC CCTGTGATGA TAGCAGTTTG CTGGAATTGA GGATTGTGTT TGAGAAGGGT 
GAGCAGGAAA ATAAAAATCT TCCGCAGGCT GTTGCCTCTG TGAAGCATCA AGAACATAGC 
ACAACAGCTT GCCCGGCGGG TCTCCCTTGT GCTTTCTTTG CACCTGTACC GGAATCATTG 
TTGCCACTCC CTGTGGATGA CCAACAAGAT GCTTTAGGCA GCGAGCAGCC AGAGACTTTG 
CAGCAAACAG TTGTGCTGCA GGATCCCAAA AGCCAGATAA GAGCCTTTAG AGACCCTCTA 
CAGGATGACA CTGGATTATA TGTTACTGAG GAAGTCACCT CCTCTGAAGA TAAACGAAAG 
ACTTATTCTT TGGCCTTTAA GCAGGAATTA AAAGATGTGA TCCTCTGTAT CTCCCCAGTA 
ATCACATTCC GAGAACCCTT TCTTTTAACT GAAAAGGGGA TGAGATGCTC TACCCGAGAT 
TATTTTGCAG AGCAGGTTTA CTGGTCTCCT CTCCTCAATA AAGAATTCAA AGAAATGGAG 
AACAGGAGGA AGAAACAGCT GCTCAGGGAT CTCTCTGGAC TTCAGGGCAT GAATGGAAGT 
ATTCAGGCCA AGTCTATTCA AGTCTTACCC TCACATGAGC TAGTGAGCAC TAGAATTGCT 
GAGCATCTGG GCGATAGCCA GAGCTTGGGT AGAATGCTGG CCGATTATCG AGCCAGAGGA 
GGAAGAATTC AGCCCAAAAA TTCAGACCCT TTTGCTCATT CAAAGGATGC ATCAAGTACT 
TCAAGTGGCC AATCAGGAAG CAAAAATGAG GGTGATGAAG AGAGAGGGCT TATTCTGAGT 
GATGCTGTGT GGTCAACAAA GGTGGACTGT CTGAATCCCA TTAATCACCA GAGACTTTGT 
GTGCTCTTCA GCAGCTCTTC TGCCCAGTCC AGCAATGCTC CTAGTGCCTG TGTCAGTCCT 
TGGATTGTAA CAATGGAATT TTATGGAAAG AATGATCTTA CATTAGGAAT ATTTTTAGAG 
AGATACTGTT TCAGGCCTTC TTATCAGTGT CCAAGCATGT TCTGTGATAC CCCCATGGTA 
CATCATATTC GGCGCTTTGT TCATGGCCAA GGCTGTGTGC AGATAATCCT GAAGGAGTTG 
GATTCTCCAG TACCTGGATA TCAGCATACA ATTCTTACAT ATTCCTGGTG TAGAATCTGC 
AAACAGGTAA CACCAGTTGT TGCTCTTTCC AATGAGTCCT GGTCTATGTC ATTTGCAAAA 
TACCTTGAAC TTAGGTTTTA TGGGCACCAG TATACTCGCA GAGCCAACGC TGAGCCCTGT 
GGTCACTCCA TCCATCATGA TTATCACCAG TATTTCTCCT ATAACCAGAT GGTGGCGTCT 
TTCAGTTATT CTCCCATTCG GCTTCTTGAA GTATGTGTTC CACTCCCCAA AATATTCATT 
AAGCGTCAGG CCCCATTAAA AGTGTCCCTT CTTCAGGATC TGAAGGACTT CTTTCAAAAA 
GTTTCACAGG TATATGTTGC CATTGATGAA AGACTTGCAT CTTTGAAAAC TGATACATTT 
AGTAAAACAA GAGAGGAAAA AATGGAAGAT ATTTTTGCAC AGAAAGAGAT GGAAGAAGGT 
GAGTTCAAGA ACTGGATTGA GAAGATGCAA GCAAGGCTCA TGTCTTCCTC TGTAGATACC 
CCTCAGCAAC TGCAGTCGGT CTTTGAGTCA CTCATTGCCA AGAAACAAAG TCTCTGTGAA 
GTGCTGCAAG CTTGGAATAA CAGGTTGCAG GACCTTTTCC AACAGGAAAA GGGTAGAAAG 
AGACCTTCAG TTCCTCCAAG TCCTGGAAGA CTGAGACAAG GGGAAGAAAG CAAGATAAGT 
GCGATGGATG CATCTCCACG GAATATTTCT CCAGGACTTC AGAATGGAGA AAAAGAGGAT 
CGCTTCTTAA CAACTTTGTC CAGCCAGAGC TCCACCAGTT CTACTCATCT CCAATTGCCT 
ACGCCACCTG AAGTCATGTC TGAACAGTCA GTGGGAGGGC CCCCTGAGCT AGATACAGCC 
AGCAGTTCCG AAGATGTGTT TGATGGGCAT TTGCTGGGAT CCACAGACAG CCAAGTGAAG 
GAAAAGTCAA CCATGAAAGC CATCTTTGCA AATTTGCTTC CAGGAAATAG CTATAATCCT 
ATTCCATTTC CTTTTGATCC AGATAAACAC TACTTAATGT ATGAACATGA ACGAGTGCCC 
ATTGCAGTCT GCGAGAAGGA ACCCAGCTCC ATCATTGCTT TTGCTCTCAG TTGTAAAGAA 
TACCGAAATG CCTTAGAGGA ATTGTCTAAA GCGACTCAGT GGAACAGTGC CGAAGAAGGG 
CTTCCAACAA ATAGTACTTC AGATAGCAGA CCAAAGAGTA GCAGCCCTAT CAGATTACCT 
GAAATGAGTG GAGGACAGAC AAATCGTACA ACAGAAACAG AACCACAACC AACCAAAAAG 
GCTTCTGGAA TGTTGTCCTT CTTCAGAGGG ACAGCAGGGA AAAGCCCCGA TCTCTCTTCC 
CAGAAGAGAG AGACCTTACG TGGAGCAGAT AGTGCTTACT ACCAGGTTGG GCAGACGGGC 
AAGGAGGGGA CCGAGAATCA AGGCGTTGAG CCTCAAGATG AAGTAGATGG AGGAGATACA 
CAAAAGAAGC AACTCATAAA TCCTCATGTG GAACTTCAAT TTTCAGATGC TAATGCCAAG 
TTTTACTGTC GGCTCTACTA TGCGGGAGAG TTTCATAAGA TGCGTGAAGT GATTCTGGAC 
AGCAGTGAAG AAGATTTCAT TCGTTCCCTC TCCCACTCAT CACCCTGGCA GGCCCGGGGA 
GGCAAATCAG GAGCTGCCTT CTATGCAACT GAGGATGATA GATTTATTTT GAAGCAAATG 
CCTCGTCTGG AAGTCCAGTC CTTCCTCGAC TTTGCACCAC ATTACTTCAA TTATATTACA 
AATGCTGTTC AACAAAAGAG GCCCACGGCG TTGGCCAAAA TTCTTGGAGT TTACAGAATT 
GGTTATAAGA ACTCTCAGAA CAACACTGAG AAGAAGTTAG ATCTCCTTGT CATGGAAAAT 
CTTTTCTACG GGAGAAAGAT GGCACAGGTT TTTGATTTGA AGGGCTCTCT TAGGAATCGG 
AATGTAAAAA CTGACACTGG AAAAGAGAGT TGTGATGTGG TCCTGCTAGA TGAAAATCTC 
CTAAAGATGG TTCGAGACAA CCCTCTATAT ATTCGTTCTC ATTCCAAAGC TGTGCTGAGA 
ACCTCGATCC ATAGTGACTC CCATTTCCTT TCTAGCCACC TCATTATAGA TTATTCTTTG 
CTGGTTGGGC GAGATGATAC TAGCAATGAG CTAGTAGTTG GAATTATAGA TTATATTCGA 
ACATTTACAT GGGACAAAAA GCTTGAGATG GTTGTGAAAT CAACAGGAAT TTTAGGTGGA 
CAAGGTAAAA TGCCAACAGT GGTGTCTCCG GAGTTGTACA GGACTAGGTT TTGTGAGGCA 
ATGGACAAGT ATTTCCTAAT GGTACCAGAC CACTGGACAG GCTTGGGTCT GAATTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PIKFYVE ( XM_017003574.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016859063.1 Homo sapiens phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X20, mRNA.

Price & Availability↑

CloneID	OHu77672
Clone ID Related Accession (Same CDS sequence)	XM_011510790.1 , XM_017003572.1 , XM_017003573.1 , XM_017003574.1
Accession Version	XM_017003574.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5340bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	1-phosphatidylinositol 3-phosphate 5-kinase isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTACCTT CATATGAGAC ATCTGTCAGT CCCCAGGCTA ACCGAACATA TGTTAGGACA 
GAGACCACTG AGGATGAACG CAAAATTCTT CTGGACAGTG TGCAGTTAAA AGACCTGTGG 
AAAAAAATCT GCCATCACAG CAGTGGAATG GAGTTTCAGG ATCACCGCTA CTGGTTGAGA 
ACGCATCCCA ACTGCATTGT AGGAAAGGAA TTAGTCAACT GGCTAATCCG AAATGGGCAT 
ATTGCCACAA GGGCACAAGC TATAGCAATT GGACAAGCAA TGGTTGATGG ACGTTGGCTG 
GATTGTGTTA GTCATCACGA CCAGCTTTTC AGAGATGAGT ATGCGCTGTA TAGACCACTG 
CAGAGTACAG AATTTTCTGA GACGCCTTCT CCCGACAGTG ACTCAGTGAA CTCCGTGGAA 
GGACACTCTG AGCCATCCTG GTTTAAAGAC ATAAAGTTTG ATGACAGTGA CACAGAACAG 
ATAGCTGAAG AAGGTGACGA TAATTTGGCT AATTCTGCCA GTCCTAGCAA GCGCACATCA 
GTCAGCAGTT TCCAGTCCAC AGTGGACAGT GACTCAGCCG CTTCTATCAG CCTGAACGTG 
GAGCTGGACA ACGTGAACTT CCATATCAAG AAGCCCTCCA AGTACCCACA TGTGCCCCCT 
CACCCTGCTG ACCAAAAAGA GTATTTGATT TCTGACACTG GAGGACAACA GCTCTCAATA 
AGTGACGCTT TCATCAAAGA ATCCTTATTT AATCGCCGAG TAGAGGAAAA ATCCAAAGAG 
CTGCCTTTCA CACCTTTGGG CTGGCATCAT AACAACCTGG AGCTCCTGAG GGAGGAGAAT 
GGGGAGAAAC AAGCCATGGA GAGGTTGCTT TCAGCTAATC ATAACCACAT GATGGCACTA 
CTCCAGCAGT TGCTCCATAG TGACTCACTG TCATCATCTT GGAGGGACAT CATCGTGTCA 
TTGGTCTGCC AGGTTGTTCA GACAGTCCGA CCTGATGTCA AGAACCAGGA TGATGACATG 
GATATCCGTC AGTTTGTCCA CATCAAAAAA ATCCCAGGTG GAAAGAAGTT TGATTCTGTG 
GTTGTCAATG GCTTTGTTTG TACCAAGAAC ATTGCACATA AAAAGATGAG TTCTTGTATT 
AAAAACCCCA AAATTCTTCT GTTGAAGTGT TCCATTGAGT ATCTCTACAG AGAAGAAACT 
AAGTTTACTT GCATTGATCC TATTGTGCTT CAGGAAAGGG AATTCTTGAA GAATTATGTC 
CAGCGAATAG TTGATGTTCG ACCCACCTTG GTTCTTGTTG AGAAAACAGT GTCTCGGATT 
GCCCAGGACA TGTTATTGGA ACATGGCATT ACTTTGGTCA TTAATGTAAA GTCACAAGTT 
TTGGAACGAA TCAGTCGAAT GACCCAAGGT GATTTAGTGA TGTCAATGGA CCAGCTGCTT 
ACGAAACCAC ACCTGGGCAC TTGTCACAAA TTTTATATGC AGATATTTCA GTTGCCTAAT 
GAACAAACCA AGACACTGAT GTTTTTTGAA GGTTGTCCAC AGCACCTAGG CTGTACAATC 
AAGCTAAGAG GAGGCTCTGA TTATGAGCTG GCTCGAGTTA AGGAGATCCT AATATTTATG 
ATCTGTGTTG CTTATCATTC TCAACTAGAA ATATCCTTTC TCATGGATGA ATTTGCTATG 
CCTCCCACAT TAATGCAAAA CCCTTCATTC CATTCCCTGA TTGAGGGACG AGGGCATGAG 
GGGGCTGTCC AAGAGCAGTA CGGTGGAGGT TCCATCCCCT GGGATCCTGA CATCCCTCCT 
GAGTCTCTGC CCTGTGATGA TAGCAGTTTG CTGGAATTGA GGATTGTGTT TGAGAAGGGT 
GAGCAGGAAA ATAAAAATCT TCCGCAGGCT GTTGCCTCTG TGAAGCATCA AGAACATAGC 
ACAACAGCTT GCCCGGCGGG TCTCCCTTGT GCTTTCTTTG CACCTGTACC GGAATCATTG 
TTGCCACTCC CTGTGGATGA CCAACAAGAT GCTTTAGGCA GCGAGCAGCC AGAGACTTTG 
CAGCAAACAG TTGTGCTGCA GGATCCCAAA AGCCAGATAA GAGCCTTTAG AGACCCTCTA 
CAGGATGACA CTGGATTATA TGTTACTGAG GAAGTCACCT CCTCTGAAGA TAAACGAAAG 
ACTTATTCTT TGGCCTTTAA GCAGGAATTA AAAGATGTGA TCCTCTGTAT CTCCCCAGTA 
ATCACATTCC GAGAACCCTT TCTTTTAACT GAAAAGGGGA TGAGATGCTC TACCCGAGAT 
TATTTTGCAG AGCAGGTTTA CTGGTCTCCT CTCCTCAATA AAGAATTCAA AGAAATGGAG 
AACAGGAGGA AGAAACAGCT GCTCAGGGAT CTCTCTGGAC TTCAGGGCAT GAATGGAAGT 
ATTCAGGCCA AGTCTATTCA AGTCTTACCC TCACATGAGC TAGTGAGCAC TAGAATTGCT 
GAGCATCTGG GCGATAGCCA GAGCTTGGGT AGAATGCTGG CCGATTATCG AGCCAGAGGA 
GGAAGAATTC AGCCCAAAAA TTCAGACCCT TTTGCTCATT CAAAGGATGC ATCAAGTACT 
TCAAGTGGCC AATCAGGAAG CAAAAATGAG GGTGATGAAG AGAGAGGGCT TATTCTGAGT 
GATGCTGTGT GGTCAACAAA GGTGGACTGT CTGAATCCCA TTAATCACCA GAGACTTTGT 
GTGCTCTTCA GCAGCTCTTC TGCCCAGTCC AGCAATGCTC CTAGTGCCTG TGTCAGTCCT 
TGGATTGTAA CAATGGAATT TTATGGAAAG AATGATCTTA CATTAGGAAT ATTTTTAGAG 
AGATACTGTT TCAGGCCTTC TTATCAGTGT CCAAGCATGT TCTGTGATAC CCCCATGGTA 
CATCATATTC GGCGCTTTGT TCATGGCCAA GGCTGTGTGC AGATAATCCT GAAGGAGTTG 
GATTCTCCAG TACCTGGATA TCAGCATACA ATTCTTACAT ATTCCTGGTG TAGAATCTGC 
AAACAGGTAA CACCAGTTGT TGCTCTTTCC AATGAGTCCT GGTCTATGTC ATTTGCAAAA 
TACCTTGAAC TTAGGTTTTA TGGGCACCAG TATACTCGCA GAGCCAACGC TGAGCCCTGT 
GGTCACTCCA TCCATCATGA TTATCACCAG TATTTCTCCT ATAACCAGAT GGTGGCGTCT 
TTCAGTTATT CTCCCATTCG GCTTCTTGAA GTATGTGTTC CACTCCCCAA AATATTCATT 
AAGCGTCAGG CCCCATTAAA AGTGTCCCTT CTTCAGGATC TGAAGGACTT CTTTCAAAAA 
GTTTCACAGG TATATGTTGC CATTGATGAA AGACTTGCAT CTTTGAAAAC TGATACATTT 
AGTAAAACAA GAGAGGAAAA AATGGAAGAT ATTTTTGCAC AGAAAGAGAT GGAAGAAGGT 
GAGTTCAAGA ACTGGATTGA GAAGATGCAA GCAAGGCTCA TGTCTTCCTC TGTAGATACC 
CCTCAGCAAC TGCAGTCGGT CTTTGAGTCA CTCATTGCCA AGAAACAAAG TCTCTGTGAA 
GTGCTGCAAG CTTGGAATAA CAGGTTGCAG GACCTTTTCC AACAGGAAAA GGGTAGAAAG 
AGACCTTCAG TTCCTCCAAG TCCTGGAAGA CTGAGACAAG GGGAAGAAAG CAAGATAAGT 
GCGATGGATG CATCTCCACG GAATATTTCT CCAGGACTTC AGAATGGAGA AAAAGAGGAT 
CGCTTCTTAA CAACTTTGTC CAGCCAGAGC TCCACCAGTT CTACTCATCT CCAATTGCCT 
ACGCCACCTG AAGTCATGTC TGAACAGTCA GTGGGAGGGC CCCCTGAGCT AGATACAGCC 
AGCAGTTCCG AAGATGTGTT TGATGGGCAT TTGCTGGGAT CCACAGACAG CCAAGTGAAG 
GAAAAGTCAA CCATGAAAGC CATCTTTGCA AATTTGCTTC CAGGAAATAG CTATAATCCT 
ATTCCATTTC CTTTTGATCC AGATAAACAC TACTTAATGT ATGAACATGA ACGAGTGCCC 
ATTGCAGTCT GCGAGAAGGA ACCCAGCTCC ATCATTGCTT TTGCTCTCAG TTGTAAAGAA 
TACCGAAATG CCTTAGAGGA ATTGTCTAAA GCGACTCAGT GGAACAGTGC CGAAGAAGGG 
CTTCCAACAA ATAGTACTTC AGATAGCAGA CCAAAGAGTA GCAGCCCTAT CAGATTACCT 
GAAATGAGTG GAGGACAGAC AAATCGTACA ACAGAAACAG AACCACAACC AACCAAAAAG 
GCTTCTGGAA TGTTGTCCTT CTTCAGAGGG ACAGCAGGGA AAAGCCCCGA TCTCTCTTCC 
CAGAAGAGAG AGACCTTACG TGGAGCAGAT AGTGCTTACT ACCAGGTTGG GCAGACGGGC 
AAGGAGGGGA CCGAGAATCA AGGCGTTGAG CCTCAAGATG AAGTAGATGG AGGAGATACA 
CAAAAGAAGC AACTCATAAA TCCTCATGTG GAACTTCAAT TTTCAGATGC TAATGCCAAG 
TTTTACTGTC GGCTCTACTA TGCGGGAGAG TTTCATAAGA TGCGTGAAGT GATTCTGGAC 
AGCAGTGAAG AAGATTTCAT TCGTTCCCTC TCCCACTCAT CACCCTGGCA GGCCCGGGGA 
GGCAAATCAG GAGCTGCCTT CTATGCAACT GAGGATGATA GATTTATTTT GAAGCAAATG 
CCTCGTCTGG AAGTCCAGTC CTTCCTCGAC TTTGCACCAC ATTACTTCAA TTATATTACA 
AATGCTGTTC AACAAAAGAG GCCCACGGCG TTGGCCAAAA TTCTTGGAGT TTACAGAATT 
GGTTATAAGA ACTCTCAGAA CAACACTGAG AAGAAGTTAG ATCTCCTTGT CATGGAAAAT 
CTTTTCTACG GGAGAAAGAT GGCACAGGTT TTTGATTTGA AGGGCTCTCT TAGGAATCGG 
AATGTAAAAA CTGACACTGG AAAAGAGAGT TGTGATGTGG TCCTGCTAGA TGAAAATCTC 
CTAAAGATGG TTCGAGACAA CCCTCTATAT ATTCGTTCTC ATTCCAAAGC TGTGCTGAGA 
ACCTCGATCC ATAGTGACTC CCATTTCCTT TCTAGCCACC TCATTATAGA TTATTCTTTG 
CTGGTTGGGC GAGATGATAC TAGCAATGAG CTAGTAGTTG GAATTATAGA TTATATTCGA 
ACATTTACAT GGGACAAAAA GCTTGAGATG GTTGTGAAAT CAACAGGAAT TTTAGGTGGA 
CAAGGTAAAA TGCCAACAGT GGTGTCTCCG GAGTTGTACA GGACTAGGTT TTGTGAGGCA 
ATGGACAAGT ATTTCCTAAT GGTACCAGAC CACTGGACAG GCTTGGGTCT GAATTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016859063.1
CDS	199..5538
Translation	MVPSYETSVSPQANRTYVRTETTEDERKILLDSVQLKDLWKKICHHSSGMEFQDHRYWLRTHPNCIVGKELVNWLIRNGHIATRAQAIAIGQAMVDGRWLDCVSHHDQLFRDEYALYRPLQSTEFSETPSPDSDSVNSVEGHSEPSWFKDIKFDDSDTEQIAEEGDDNLANSASPSKRTSVSSFQSTVDSDSAASISLNVELDNVNFHIKKPSKYPHVPPHPADQKEYLISDTGGQQLSISDAFIKESLFNRRVEEKSKELPFTPLGWHHNNLELLREENGEKQAMERLLSANHNHMMALLQQLLHSDSLSSSWRDIIVSLVCQVVQTVRPDVKNQDDDMDIRQFVHIKKIPGGKKFDSVVVNGFVCTKNIAHKKMSSCIKNPKILLLKCSIEYLYREETKFTCIDPIVLQEREFLKNYVQRIVDVRPTLVLVEKTVSRIAQDMLLEHGITLVINVKSQVLERISRMTQGDLVMSMDQLLTKPHLGTCHKFYMQIFQLPNEQTKTLMFFEGCPQHLGCTIKLRGGSDYELARVKEILIFMICVAYHSQLEISFLMDEFAMPPTLMQNPSFHSLIEGRGHEGAVQEQYGGGSIPWDPDIPPESLPCDDSSLLELRIVFEKGEQENKNLPQAVASVKHQEHSTTACPAGLPCAFFAPVPESLLPLPVDDQQDALGSEQPETLQQTVVLQDPKSQIRAFRDPLQDDTGLYVTEEVTSSEDKRKTYSLAFKQELKDVILCISPVITFREPFLLTEKGMRCSTRDYFAEQVYWSPLLNKEFKEMENRRKKQLLRDLSGLQGMNGSIQAKSIQVLPSHELVSTRIAEHLGDSQSLGRMLADYRARGGRIQPKNSDPFAHSKDASSTSSGQSGSKNEGDEERGLILSDAVWSTKVDCLNPINHQRLCVLFSSSSAQSSNAPSACVSPWIVTMEFYGKNDLTLGIFLERYCFRPSYQCPSMFCDTPMVHHIRRFVHGQGCVQIILKELDSPVPGYQHTILTYSWCRICKQVTPVVALSNESWSMSFAKYLELRFYGHQYTRRANAEPCGHSIHHDYHQYFSYNQMVASFSYSPIRLLEVCVPLPKIFIKRQAPLKVSLLQDLKDFFQKVSQVYVAIDERLASLKTDTFSKTREEKMEDIFAQKEMEEGEFKNWIEKMQARLMSSSVDTPQQLQSVFESLIAKKQSLCEVLQAWNNRLQDLFQQEKGRKRPSVPPSPGRLRQGEESKISAMDASPRNISPGLQNGEKEDRFLTTLSSQSSTSSTHLQLPTPPEVMSEQSVGGPPELDTASSSEDVFDGHLLGSTDSQVKEKSTMKAIFANLLPGNSYNPIPFPFDPDKHYLMYEHERVPIAVCEKEPSSIIAFALSCKEYRNALEELSKATQWNSAEEGLPTNSTSDSRPKSSSPIRLPEMSGGQTNRTTETEPQPTKKASGMLSFFRGTAGKSPDLSSQKRETLRGADSAYYQVGQTGKEGTENQGVEPQDEVDGGDTQKKQLINPHVELQFSDANAKFYCRLYYAGEFHKMREVILDSSEEDFIRSLSHSSPWQARGGKSGAAFYATEDDRFILKQMPRLEVQSFLDFAPHYFNYITNAVQQKRPTALAKILGVYRIGYKNSQNNTEKKLDLLVMENLFYGRKMAQVFDLKGSLRNRNVKTDTGKESCDVVLLDENLLKMVRDNPLYIRSHSKAVLRTSIHSDSHFLSSHLIIDYSLLVGRDDTSNELVVGIIDYIRTFTWDKKLEMVVKSTGILGGQGKMPTVVSPELYRTRFCEAMDKYFLMVPDHWTGLGLNC

Target ORF information:

RefSeq Version	XM_017003574.1
Organism	Homo sapiens(human)
Definition	Homo sapiens phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X20, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017003574.1

ORF Insert Sequence:

ATGGTACCTT CATATGAGAC ATCTGTCAGT CCCCAGGCTA ACCGAACATA TGTTAGGACA 
GAGACCACTG AGGATGAACG CAAAATTCTT CTGGACAGTG TGCAGTTAAA AGACCTGTGG 
AAAAAAATCT GCCATCACAG CAGTGGAATG GAGTTTCAGG ATCACCGCTA CTGGTTGAGA 
ACGCATCCCA ACTGCATTGT AGGAAAGGAA TTAGTCAACT GGCTAATCCG AAATGGGCAT 
ATTGCCACAA GGGCACAAGC TATAGCAATT GGACAAGCAA TGGTTGATGG ACGTTGGCTG 
GATTGTGTTA GTCATCACGA CCAGCTTTTC AGAGATGAGT ATGCGCTGTA TAGACCACTG 
CAGAGTACAG AATTTTCTGA GACGCCTTCT CCCGACAGTG ACTCAGTGAA CTCCGTGGAA 
GGACACTCTG AGCCATCCTG GTTTAAAGAC ATAAAGTTTG ATGACAGTGA CACAGAACAG 
ATAGCTGAAG AAGGTGACGA TAATTTGGCT AATTCTGCCA GTCCTAGCAA GCGCACATCA 
GTCAGCAGTT TCCAGTCCAC AGTGGACAGT GACTCAGCCG CTTCTATCAG CCTGAACGTG 
GAGCTGGACA ACGTGAACTT CCATATCAAG AAGCCCTCCA AGTACCCACA TGTGCCCCCT 
CACCCTGCTG ACCAAAAAGA GTATTTGATT TCTGACACTG GAGGACAACA GCTCTCAATA 
AGTGACGCTT TCATCAAAGA ATCCTTATTT AATCGCCGAG TAGAGGAAAA ATCCAAAGAG 
CTGCCTTTCA CACCTTTGGG CTGGCATCAT AACAACCTGG AGCTCCTGAG GGAGGAGAAT 
GGGGAGAAAC AAGCCATGGA GAGGTTGCTT TCAGCTAATC ATAACCACAT GATGGCACTA 
CTCCAGCAGT TGCTCCATAG TGACTCACTG TCATCATCTT GGAGGGACAT CATCGTGTCA 
TTGGTCTGCC AGGTTGTTCA GACAGTCCGA CCTGATGTCA AGAACCAGGA TGATGACATG 
GATATCCGTC AGTTTGTCCA CATCAAAAAA ATCCCAGGTG GAAAGAAGTT TGATTCTGTG 
GTTGTCAATG GCTTTGTTTG TACCAAGAAC ATTGCACATA AAAAGATGAG TTCTTGTATT 
AAAAACCCCA AAATTCTTCT GTTGAAGTGT TCCATTGAGT ATCTCTACAG AGAAGAAACT 
AAGTTTACTT GCATTGATCC TATTGTGCTT CAGGAAAGGG AATTCTTGAA GAATTATGTC 
CAGCGAATAG TTGATGTTCG ACCCACCTTG GTTCTTGTTG AGAAAACAGT GTCTCGGATT 
GCCCAGGACA TGTTATTGGA ACATGGCATT ACTTTGGTCA TTAATGTAAA GTCACAAGTT 
TTGGAACGAA TCAGTCGAAT GACCCAAGGT GATTTAGTGA TGTCAATGGA CCAGCTGCTT 
ACGAAACCAC ACCTGGGCAC TTGTCACAAA TTTTATATGC AGATATTTCA GTTGCCTAAT 
GAACAAACCA AGACACTGAT GTTTTTTGAA GGTTGTCCAC AGCACCTAGG CTGTACAATC 
AAGCTAAGAG GAGGCTCTGA TTATGAGCTG GCTCGAGTTA AGGAGATCCT AATATTTATG 
ATCTGTGTTG CTTATCATTC TCAACTAGAA ATATCCTTTC TCATGGATGA ATTTGCTATG 
CCTCCCACAT TAATGCAAAA CCCTTCATTC CATTCCCTGA TTGAGGGACG AGGGCATGAG 
GGGGCTGTCC AAGAGCAGTA CGGTGGAGGT TCCATCCCCT GGGATCCTGA CATCCCTCCT 
GAGTCTCTGC CCTGTGATGA TAGCAGTTTG CTGGAATTGA GGATTGTGTT TGAGAAGGGT 
GAGCAGGAAA ATAAAAATCT TCCGCAGGCT GTTGCCTCTG TGAAGCATCA AGAACATAGC 
ACAACAGCTT GCCCGGCGGG TCTCCCTTGT GCTTTCTTTG CACCTGTACC GGAATCATTG 
TTGCCACTCC CTGTGGATGA CCAACAAGAT GCTTTAGGCA GCGAGCAGCC AGAGACTTTG 
CAGCAAACAG TTGTGCTGCA GGATCCCAAA AGCCAGATAA GAGCCTTTAG AGACCCTCTA 
CAGGATGACA CTGGATTATA TGTTACTGAG GAAGTCACCT CCTCTGAAGA TAAACGAAAG 
ACTTATTCTT TGGCCTTTAA GCAGGAATTA AAAGATGTGA TCCTCTGTAT CTCCCCAGTA 
ATCACATTCC GAGAACCCTT TCTTTTAACT GAAAAGGGGA TGAGATGCTC TACCCGAGAT 
TATTTTGCAG AGCAGGTTTA CTGGTCTCCT CTCCTCAATA AAGAATTCAA AGAAATGGAG 
AACAGGAGGA AGAAACAGCT GCTCAGGGAT CTCTCTGGAC TTCAGGGCAT GAATGGAAGT 
ATTCAGGCCA AGTCTATTCA AGTCTTACCC TCACATGAGC TAGTGAGCAC TAGAATTGCT 
GAGCATCTGG GCGATAGCCA GAGCTTGGGT AGAATGCTGG CCGATTATCG AGCCAGAGGA 
GGAAGAATTC AGCCCAAAAA TTCAGACCCT TTTGCTCATT CAAAGGATGC ATCAAGTACT 
TCAAGTGGCC AATCAGGAAG CAAAAATGAG GGTGATGAAG AGAGAGGGCT TATTCTGAGT 
GATGCTGTGT GGTCAACAAA GGTGGACTGT CTGAATCCCA TTAATCACCA GAGACTTTGT 
GTGCTCTTCA GCAGCTCTTC TGCCCAGTCC AGCAATGCTC CTAGTGCCTG TGTCAGTCCT 
TGGATTGTAA CAATGGAATT TTATGGAAAG AATGATCTTA CATTAGGAAT ATTTTTAGAG 
AGATACTGTT TCAGGCCTTC TTATCAGTGT CCAAGCATGT TCTGTGATAC CCCCATGGTA 
CATCATATTC GGCGCTTTGT TCATGGCCAA GGCTGTGTGC AGATAATCCT GAAGGAGTTG 
GATTCTCCAG TACCTGGATA TCAGCATACA ATTCTTACAT ATTCCTGGTG TAGAATCTGC 
AAACAGGTAA CACCAGTTGT TGCTCTTTCC AATGAGTCCT GGTCTATGTC ATTTGCAAAA 
TACCTTGAAC TTAGGTTTTA TGGGCACCAG TATACTCGCA GAGCCAACGC TGAGCCCTGT 
GGTCACTCCA TCCATCATGA TTATCACCAG TATTTCTCCT ATAACCAGAT GGTGGCGTCT 
TTCAGTTATT CTCCCATTCG GCTTCTTGAA GTATGTGTTC CACTCCCCAA AATATTCATT 
AAGCGTCAGG CCCCATTAAA AGTGTCCCTT CTTCAGGATC TGAAGGACTT CTTTCAAAAA 
GTTTCACAGG TATATGTTGC CATTGATGAA AGACTTGCAT CTTTGAAAAC TGATACATTT 
AGTAAAACAA GAGAGGAAAA AATGGAAGAT ATTTTTGCAC AGAAAGAGAT GGAAGAAGGT 
GAGTTCAAGA ACTGGATTGA GAAGATGCAA GCAAGGCTCA TGTCTTCCTC TGTAGATACC 
CCTCAGCAAC TGCAGTCGGT CTTTGAGTCA CTCATTGCCA AGAAACAAAG TCTCTGTGAA 
GTGCTGCAAG CTTGGAATAA CAGGTTGCAG GACCTTTTCC AACAGGAAAA GGGTAGAAAG 
AGACCTTCAG TTCCTCCAAG TCCTGGAAGA CTGAGACAAG GGGAAGAAAG CAAGATAAGT 
GCGATGGATG CATCTCCACG GAATATTTCT CCAGGACTTC AGAATGGAGA AAAAGAGGAT 
CGCTTCTTAA CAACTTTGTC CAGCCAGAGC TCCACCAGTT CTACTCATCT CCAATTGCCT 
ACGCCACCTG AAGTCATGTC TGAACAGTCA GTGGGAGGGC CCCCTGAGCT AGATACAGCC 
AGCAGTTCCG AAGATGTGTT TGATGGGCAT TTGCTGGGAT CCACAGACAG CCAAGTGAAG 
GAAAAGTCAA CCATGAAAGC CATCTTTGCA AATTTGCTTC CAGGAAATAG CTATAATCCT 
ATTCCATTTC CTTTTGATCC AGATAAACAC TACTTAATGT ATGAACATGA ACGAGTGCCC 
ATTGCAGTCT GCGAGAAGGA ACCCAGCTCC ATCATTGCTT TTGCTCTCAG TTGTAAAGAA 
TACCGAAATG CCTTAGAGGA ATTGTCTAAA GCGACTCAGT GGAACAGTGC CGAAGAAGGG 
CTTCCAACAA ATAGTACTTC AGATAGCAGA CCAAAGAGTA GCAGCCCTAT CAGATTACCT 
GAAATGAGTG GAGGACAGAC AAATCGTACA ACAGAAACAG AACCACAACC AACCAAAAAG 
GCTTCTGGAA TGTTGTCCTT CTTCAGAGGG ACAGCAGGGA AAAGCCCCGA TCTCTCTTCC 
CAGAAGAGAG AGACCTTACG TGGAGCAGAT AGTGCTTACT ACCAGGTTGG GCAGACGGGC 
AAGGAGGGGA CCGAGAATCA AGGCGTTGAG CCTCAAGATG AAGTAGATGG AGGAGATACA 
CAAAAGAAGC AACTCATAAA TCCTCATGTG GAACTTCAAT TTTCAGATGC TAATGCCAAG 
TTTTACTGTC GGCTCTACTA TGCGGGAGAG TTTCATAAGA TGCGTGAAGT GATTCTGGAC 
AGCAGTGAAG AAGATTTCAT TCGTTCCCTC TCCCACTCAT CACCCTGGCA GGCCCGGGGA 
GGCAAATCAG GAGCTGCCTT CTATGCAACT GAGGATGATA GATTTATTTT GAAGCAAATG 
CCTCGTCTGG AAGTCCAGTC CTTCCTCGAC TTTGCACCAC ATTACTTCAA TTATATTACA 
AATGCTGTTC AACAAAAGAG GCCCACGGCG TTGGCCAAAA TTCTTGGAGT TTACAGAATT 
GGTTATAAGA ACTCTCAGAA CAACACTGAG AAGAAGTTAG ATCTCCTTGT CATGGAAAAT 
CTTTTCTACG GGAGAAAGAT GGCACAGGTT TTTGATTTGA AGGGCTCTCT TAGGAATCGG 
AATGTAAAAA CTGACACTGG AAAAGAGAGT TGTGATGTGG TCCTGCTAGA TGAAAATCTC 
CTAAAGATGG TTCGAGACAA CCCTCTATAT ATTCGTTCTC ATTCCAAAGC TGTGCTGAGA 
ACCTCGATCC ATAGTGACTC CCATTTCCTT TCTAGCCACC TCATTATAGA TTATTCTTTG 
CTGGTTGGGC GAGATGATAC TAGCAATGAG CTAGTAGTTG GAATTATAGA TTATATTCGA 
ACATTTACAT GGGACAAAAA GCTTGAGATG GTTGTGAAAT CAACAGGAAT TTTAGGTGGA 
CAAGGTAAAA TGCCAACAGT GGTGTCTCCG GAGTTGTACA GGACTAGGTT TTGTGAGGCA 
ATGGACAAGT ATTTCCTAAT GGTACCAGAC CACTGGACAG GCTTGGGTCT GAATTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PIKFYVE ( XM_011510792.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011509094.1 Homo sapiens phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X21, mRNA.

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CloneID	OHu77673
Clone ID Related Accession (Same CDS sequence)	XM_011510792.3 , XM_011510792.2
Accession Version	XM_011510792.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4023bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	1-phosphatidylinositol 3-phosphate 5-kinase isoform X17
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011510792.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG ATGATAAGAC GTCCCCAACA CTGGACTCTG CTAATGATTT GCCTCGATCT 
CCTACTAGTC CTTCTCATCT CACACACTTT AAACCTTTGA CTCCTGATCA AGATGAGCCC 
CCTTTTAAAT CAGCTTATAG TTCTTTTGTA AATCTCTTTC GTTTTAACAA AGAGAGAGCA 
GAAGGAGGCC AGGGAGAACA GCAGCCTTTG AGTGGAAGTT GGACCAGCCC TCAGCTCCCT 
TCGAGGACAC AGTCTGTTAG GTCACCCACA CCTTATAAAA AGCAGCTTAA TGAGGAACTC 
CAGCGGCGCT CTTCAGCATT AGCAGACAAC AGTTTGCAAC ATCCCCAGGA GAACACAGAC 
ACAAGAAGGA AAGCAGAACC TACCTTTGGA GGTCATGACC CTCGTACAGC TGTTCAGCTT 
CGAAGCCTCA GCACAGTATT AAAACGCCTC AAGGAAATCA TGGAGGGGAA AAGCCAGGAT 
AGTGACCTGA AACAATACTG GATGCCAGAT AGCCAATGTA AAGAGTGCTA TGACTGTAGT 
GAGAAATTTA CAACCTTTAG GCGCAGACAC CATTGCCGAC TATGTGGGCA GATTTTCTGC 
AGTCGTTGCT GTAATCAAGA AATCCCTGGA AAATTTATGG GCTATACAGG AGACCTCCGA 
GCTTGCACAT ATTGTAGAAA AATAGCCTTA AGTTATGCTC ATTCCACAGA CAGTAATTCT 
ATTGGGGAAG ACTTGAATGC TCTTTCAGAT TCTGCTTGCT CTGTGTCTGT GCTTGATCCA 
AGTGAACCCC GAACACCTGT TGGGAGTAGG AAAGCCAGCC GTAACATATT TTTAGAGGAT 
GATTTGGCCT GGCAAAGTTT GATTCATCCA GATTCCTCAA ATACTCCTCT TTCAACAAGA 
CTTGTATCTG TGCAAGAGGA TGCTGGGAAA TCTCCTGCTC GAAATAGATC AGCCAGCATT 
ACTAACCTGT CACTGGATAG ATCTGGTTCT CCTATGGTAC CTTCATATGA GACATCTGTC 
AGTCCCCAGG CTAACCGAAC ATATGTTAGG ACAGAGACCA CTGAGGATGA ACGCAAAATT 
CTTCTGGACA GTGTGCAGTT AAAAGACCTG TGGAAAAAAA TCTGCCATCA CAGCAGTGGA 
ATGGAGTTTC AGGATCACCG CTACTGGTTG AGAACGCATC CCAACTGCAT TGTAGGAAAG 
GAATTAGTCA ACTGGCTAAT CCGAAATGGG CATATTGCCA CAAGGGCACA AGCTATAGCA 
ATTGGACAAG CAATGGTTGA TGGACGTTGG CTGGATTGTG TTAGTCATCA CGACCAGCTT 
TTCAGAGATG AGTATGCGCT GTATAGACCA CTGCAGAGTA CAGAATTTTC TGAGACGCCT 
TCTCCCGACA GTGACTCAGT GAACTCCGTG GAAGGACACT CTGAGCCATC CTGGTTTAAA 
GACATAAAGT TTGATGACAG TGACACAGAA CAGATAGCTG AAGAAGGTGA CGATAATTTG 
GCTAATTCTG CCAGTCCTAG CAAGCGCACA TCAGTCAGCA GTTTCCAGTC CACAGTGGAC 
AGTGACTCAG CCGCTTCTAT CAGCCTGAAC GTGGAGCTGG ACAACGTGAA CTTCCATATC 
AAGAAGCCCT CCAAGTACCC ACATGTGCCC CCTCACCCTG CTGACCAAAA AGAGTATTTG 
ATTTCTGACA CTGGAGGACA ACAGCTCTCA ATAAGTGACG CTTTCATCAA AGAATCCTTA 
TTTAATCGCC GAGTAGAGGA AAAATCCAAA GAGCTGCCTT TCACACCTTT GGGCTGGCAT 
CATAACAACC TGGAGCTCCT GAGGGAGGAG AATGGGGAGA AACAAGCCAT GGAGAGGTTG 
CTTTCAGCTA ATCATAACCA CATGATGGCA CTACTCCAGC AGTTGCTCCA TAGTGACTCA 
CTGTCATCAT CTTGGAGGGA CATCATCGTG TCATTGGTCT GCCAGGTTGT TCAGACAGTC 
CGACCTGATG TCAAGAACCA GGATGATGAC ATGGATATCC GTCAGTTTGT CCACATCAAA 
AAAATCCCAG GTGGAAAGAA GTTTGATTCT GTGGTTGTCA ATGGCTTTGT TTGTACCAAG 
AACATTGCAC ATAAAAAGAT GAGTTCTTGT ATTAAAAACC CCAAAATTCT TCTGTTGAAG 
TGTTCCATTG AGTATCTCTA CAGAGAAGAA ACTAAGTTTA CTTGCATTGA TCCTATTGTG 
CTTCAGGAAA GGGAATTCTT GAAGAATTAT GTCCAGCGAA TAGTTGATGT TCGACCCACC 
TTGGTTCTTG TTGAGAAAAC AGTGTCTCGG ATTGCCCAGG ACATGTTATT GGAACATGGC 
ATTACTTTGG TCATTAATGT AAAGTCACAA GTTTTGGAAC GAATCAGTCG AATGACCCAA 
GGTGATTTAG TGATGTCAAT GGACCAGCTG CTTACGAAAC CACACCTGGG CACTTGTCAC 
AAATTTTATA TGCAGATATT TCAGTTGCCT AATGAACAAA CCAAGACACT GATGTTTTTT 
GAAGGTTGTC CACAGCACCT AGGCTGTACA ATCAAGCTAA GAGGAGGCTC TGATTATGAG 
CTGGCTCGAG TTAAGGAGAT CCTAATATTT ATGATCTGTG TTGCTTATCA TTCTCAACTA 
GAAATATCCT TTCTCATGGA TGAATTTGCT ATGCCTCCCA CATTAATGCA AAACCCTTCA 
TTCCATTCCC TGATTGAGGG ACGAGGGCAT GAGGGGGCTG TCCAAGAGCA GTACGGTGGA 
GGTTCCATCC CCTGGGATCC TGACATCCCT CCTGAGTCTC TGCCCTGTGA TGATAGCAGT 
TTGCTGGAAT TGAGGATTGT GTTTGAGAAG GGTGAGCAGG AAAATAAAAA TCTTCCGCAG 
GCTGTTGCCT CTGTGAAGCA TCAAGAACAT AGCACAACAG CTTGCCCGGC GGGTCTCCCT 
TGTGCTTTCT TTGCACCTGT ACCGGAATCA TTGTTGCCAC TCCCTGTGGA TGACCAACAA 
GATGCTTTAG GCAGCGAGCA GCCAGAGACT TTGCAGCAAA CAGTTGTGCT GCAGGATCCC 
AAAAGCCAGA TAAGAGCCTT TAGAGACCCT CTACAGGATG ACACTGGATT ATATGTTACT 
GAGGAAGTCA CCTCCTCTGA AGATAAACGA AAGACTTATT CTTTGGCCTT TAAGCAGGAA 
TTAAAAGATG TGATCCTCTG TATCTCCCCA GTAATCACAT TCCGAGAACC CTTTCTTTTA 
ACTGAAAAGG GGATGAGATG CTCTACCCGA GATTATTTTG CAGAGCAGGT TTACTGGTCT 
CCTCTCCTCA ATAAAGAATT CAAAGAAATG GAGAACAGGA GGAAGAAACA GCTGCTCAGG 
GATCTCTCTG GACTTCAGGG CATGAATGGA AGTATTCAGG CCAAGTCTAT TCAAGTCTTA 
CCCTCACATG AGCTAGTGAG CACTAGAATT GCTGAGCATC TGGGCGATAG CCAGAGCTTG 
GGTAGAATGC TGGCCGATTA TCGAGCCAGA GGAGGAAGAA TTCAGCCCAA AAATTCAGAC 
CCTTTTGCTC ATTCAAAGGA TGCATCAAGT ACTTCAAGTG GCCAATCAGG AAGCAAAAAT 
GAGGGTGATG AAGAGAGAGG GCTTATTCTG AGTGATGCTG TGTGGTCAAC AAAGGTGGAC 
TGTCTGAATC CCATTAATCA CCAGAGACTT TGTGTGCTCT TCAGCAGCTC TTCTGCCCAG 
TCCAGCAATG CTCCTAGTGC CTGTGTCAGT CCTTGGATTG TAACAATGGA ATTTTATGGA 
AAGAATGATC TTACATTAGG AATATTTTTA GAGAGATACT GTTTCAGGCC TTCTTATCAG 
TGTCCAAGCA TGTTCTGTGA TACCCCCATG GTACATCATA TTCGGCGCTT TGTTCATGGC 
CAAGGCTGTG TGCAGATAAT CCTGAAGGAG TTGGATTCTC CAGTACCTGG ATATCAGCAT 
ACAATTCTTA CATATTCCTG GTGTAGAATC TGCAAACAGT TATTCTCCCA TTCGGCTTCT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011509094.1
CDS	182..4204
Translation	MATDDKTSPTLDSANDLPRSPTSPSHLTHFKPLTPDQDEPPFKSAYSSFVNLFRFNKERAEGGQGEQQPLSGSWTSPQLPSRTQSVRSPTPYKKQLNEELQRRSSALADNSLQHPQENTDTRRKAEPTFGGHDPRTAVQLRSLSTVLKRLKEIMEGKSQDSDLKQYWMPDSQCKECYDCSEKFTTFRRRHHCRLCGQIFCSRCCNQEIPGKFMGYTGDLRACTYCRKIALSYAHSTDSNSIGEDLNALSDSACSVSVLDPSEPRTPVGSRKASRNIFLEDDLAWQSLIHPDSSNTPLSTRLVSVQEDAGKSPARNRSASITNLSLDRSGSPMVPSYETSVSPQANRTYVRTETTEDERKILLDSVQLKDLWKKICHHSSGMEFQDHRYWLRTHPNCIVGKELVNWLIRNGHIATRAQAIAIGQAMVDGRWLDCVSHHDQLFRDEYALYRPLQSTEFSETPSPDSDSVNSVEGHSEPSWFKDIKFDDSDTEQIAEEGDDNLANSASPSKRTSVSSFQSTVDSDSAASISLNVELDNVNFHIKKPSKYPHVPPHPADQKEYLISDTGGQQLSISDAFIKESLFNRRVEEKSKELPFTPLGWHHNNLELLREENGEKQAMERLLSANHNHMMALLQQLLHSDSLSSSWRDIIVSLVCQVVQTVRPDVKNQDDDMDIRQFVHIKKIPGGKKFDSVVVNGFVCTKNIAHKKMSSCIKNPKILLLKCSIEYLYREETKFTCIDPIVLQEREFLKNYVQRIVDVRPTLVLVEKTVSRIAQDMLLEHGITLVINVKSQVLERISRMTQGDLVMSMDQLLTKPHLGTCHKFYMQIFQLPNEQTKTLMFFEGCPQHLGCTIKLRGGSDYELARVKEILIFMICVAYHSQLEISFLMDEFAMPPTLMQNPSFHSLIEGRGHEGAVQEQYGGGSIPWDPDIPPESLPCDDSSLLELRIVFEKGEQENKNLPQAVASVKHQEHSTTACPAGLPCAFFAPVPESLLPLPVDDQQDALGSEQPETLQQTVVLQDPKSQIRAFRDPLQDDTGLYVTEEVTSSEDKRKTYSLAFKQELKDVILCISPVITFREPFLLTEKGMRCSTRDYFAEQVYWSPLLNKEFKEMENRRKKQLLRDLSGLQGMNGSIQAKSIQVLPSHELVSTRIAEHLGDSQSLGRMLADYRARGGRIQPKNSDPFAHSKDASSTSSGQSGSKNEGDEERGLILSDAVWSTKVDCLNPINHQRLCVLFSSSSAQSSNAPSACVSPWIVTMEFYGKNDLTLGIFLERYCFRPSYQCPSMFCDTPMVHHIRRFVHGQGCVQIILKELDSPVPGYQHTILTYSWCRICKQLFSHSAS

Target ORF information:

RefSeq Version	XM_011510792.3
Organism	Homo sapiens(human)
Definition	Homo sapiens phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X21, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011510792.3

ORF Insert Sequence:

ATGGCCACAG ATGATAAGAC GTCCCCAACA CTGGACTCTG CTAATGATTT GCCTCGATCT 
CCTACTAGTC CTTCTCATCT CACACACTTT AAACCTTTGA CTCCTGATCA AGATGAGCCC 
CCTTTTAAAT CAGCTTATAG TTCTTTTGTA AATCTCTTTC GTTTTAACAA AGAGAGAGCA 
GAAGGAGGCC AGGGAGAACA GCAGCCTTTG AGTGGAAGTT GGACCAGCCC TCAGCTCCCT 
TCGAGGACAC AGTCTGTTAG GTCACCCACA CCTTATAAAA AGCAGCTTAA TGAGGAACTC 
CAGCGGCGCT CTTCAGCATT AGCAGACAAC AGTTTGCAAC ATCCCCAGGA GAACACAGAC 
ACAAGAAGGA AAGCAGAACC TACCTTTGGA GGTCATGACC CTCGTACAGC TGTTCAGCTT 
CGAAGCCTCA GCACAGTATT AAAACGCCTC AAGGAAATCA TGGAGGGGAA AAGCCAGGAT 
AGTGACCTGA AACAATACTG GATGCCAGAT AGCCAATGTA AAGAGTGCTA TGACTGTAGT 
GAGAAATTTA CAACCTTTAG GCGCAGACAC CATTGCCGAC TATGTGGGCA GATTTTCTGC 
AGTCGTTGCT GTAATCAAGA AATCCCTGGA AAATTTATGG GCTATACAGG AGACCTCCGA 
GCTTGCACAT ATTGTAGAAA AATAGCCTTA AGTTATGCTC ATTCCACAGA CAGTAATTCT 
ATTGGGGAAG ACTTGAATGC TCTTTCAGAT TCTGCTTGCT CTGTGTCTGT GCTTGATCCA 
AGTGAACCCC GAACACCTGT TGGGAGTAGG AAAGCCAGCC GTAACATATT TTTAGAGGAT 
GATTTGGCCT GGCAAAGTTT GATTCATCCA GATTCCTCAA ATACTCCTCT TTCAACAAGA 
CTTGTATCTG TGCAAGAGGA TGCTGGGAAA TCTCCTGCTC GAAATAGATC AGCCAGCATT 
ACTAACCTGT CACTGGATAG ATCTGGTTCT CCTATGGTAC CTTCATATGA GACATCTGTC 
AGTCCCCAGG CTAACCGAAC ATATGTTAGG ACAGAGACCA CTGAGGATGA ACGCAAAATT 
CTTCTGGACA GTGTGCAGTT AAAAGACCTG TGGAAAAAAA TCTGCCATCA CAGCAGTGGA 
ATGGAGTTTC AGGATCACCG CTACTGGTTG AGAACGCATC CCAACTGCAT TGTAGGAAAG 
GAATTAGTCA ACTGGCTAAT CCGAAATGGG CATATTGCCA CAAGGGCACA AGCTATAGCA 
ATTGGACAAG CAATGGTTGA TGGACGTTGG CTGGATTGTG TTAGTCATCA CGACCAGCTT 
TTCAGAGATG AGTATGCGCT GTATAGACCA CTGCAGAGTA CAGAATTTTC TGAGACGCCT 
TCTCCCGACA GTGACTCAGT GAACTCCGTG GAAGGACACT CTGAGCCATC CTGGTTTAAA 
GACATAAAGT TTGATGACAG TGACACAGAA CAGATAGCTG AAGAAGGTGA CGATAATTTG 
GCTAATTCTG CCAGTCCTAG CAAGCGCACA TCAGTCAGCA GTTTCCAGTC CACAGTGGAC 
AGTGACTCAG CCGCTTCTAT CAGCCTGAAC GTGGAGCTGG ACAACGTGAA CTTCCATATC 
AAGAAGCCCT CCAAGTACCC ACATGTGCCC CCTCACCCTG CTGACCAAAA AGAGTATTTG 
ATTTCTGACA CTGGAGGACA ACAGCTCTCA ATAAGTGACG CTTTCATCAA AGAATCCTTA 
TTTAATCGCC GAGTAGAGGA AAAATCCAAA GAGCTGCCTT TCACACCTTT GGGCTGGCAT 
CATAACAACC TGGAGCTCCT GAGGGAGGAG AATGGGGAGA AACAAGCCAT GGAGAGGTTG 
CTTTCAGCTA ATCATAACCA CATGATGGCA CTACTCCAGC AGTTGCTCCA TAGTGACTCA 
CTGTCATCAT CTTGGAGGGA CATCATCGTG TCATTGGTCT GCCAGGTTGT TCAGACAGTC 
CGACCTGATG TCAAGAACCA GGATGATGAC ATGGATATCC GTCAGTTTGT CCACATCAAA 
AAAATCCCAG GTGGAAAGAA GTTTGATTCT GTGGTTGTCA ATGGCTTTGT TTGTACCAAG 
AACATTGCAC ATAAAAAGAT GAGTTCTTGT ATTAAAAACC CCAAAATTCT TCTGTTGAAG 
TGTTCCATTG AGTATCTCTA CAGAGAAGAA ACTAAGTTTA CTTGCATTGA TCCTATTGTG 
CTTCAGGAAA GGGAATTCTT GAAGAATTAT GTCCAGCGAA TAGTTGATGT TCGACCCACC 
TTGGTTCTTG TTGAGAAAAC AGTGTCTCGG ATTGCCCAGG ACATGTTATT GGAACATGGC 
ATTACTTTGG TCATTAATGT AAAGTCACAA GTTTTGGAAC GAATCAGTCG AATGACCCAA 
GGTGATTTAG TGATGTCAAT GGACCAGCTG CTTACGAAAC CACACCTGGG CACTTGTCAC 
AAATTTTATA TGCAGATATT TCAGTTGCCT AATGAACAAA CCAAGACACT GATGTTTTTT 
GAAGGTTGTC CACAGCACCT AGGCTGTACA ATCAAGCTAA GAGGAGGCTC TGATTATGAG 
CTGGCTCGAG TTAAGGAGAT CCTAATATTT ATGATCTGTG TTGCTTATCA TTCTCAACTA 
GAAATATCCT TTCTCATGGA TGAATTTGCT ATGCCTCCCA CATTAATGCA AAACCCTTCA 
TTCCATTCCC TGATTGAGGG ACGAGGGCAT GAGGGGGCTG TCCAAGAGCA GTACGGTGGA 
GGTTCCATCC CCTGGGATCC TGACATCCCT CCTGAGTCTC TGCCCTGTGA TGATAGCAGT 
TTGCTGGAAT TGAGGATTGT GTTTGAGAAG GGTGAGCAGG AAAATAAAAA TCTTCCGCAG 
GCTGTTGCCT CTGTGAAGCA TCAAGAACAT AGCACAACAG CTTGCCCGGC GGGTCTCCCT 
TGTGCTTTCT TTGCACCTGT ACCGGAATCA TTGTTGCCAC TCCCTGTGGA TGACCAACAA 
GATGCTTTAG GCAGCGAGCA GCCAGAGACT TTGCAGCAAA CAGTTGTGCT GCAGGATCCC 
AAAAGCCAGA TAAGAGCCTT TAGAGACCCT CTACAGGATG ACACTGGATT ATATGTTACT 
GAGGAAGTCA CCTCCTCTGA AGATAAACGA AAGACTTATT CTTTGGCCTT TAAGCAGGAA 
TTAAAAGATG TGATCCTCTG TATCTCCCCA GTAATCACAT TCCGAGAACC CTTTCTTTTA 
ACTGAAAAGG GGATGAGATG CTCTACCCGA GATTATTTTG CAGAGCAGGT TTACTGGTCT 
CCTCTCCTCA ATAAAGAATT CAAAGAAATG GAGAACAGGA GGAAGAAACA GCTGCTCAGG 
GATCTCTCTG GACTTCAGGG CATGAATGGA AGTATTCAGG CCAAGTCTAT TCAAGTCTTA 
CCCTCACATG AGCTAGTGAG CACTAGAATT GCTGAGCATC TGGGCGATAG CCAGAGCTTG 
GGTAGAATGC TGGCCGATTA TCGAGCCAGA GGAGGAAGAA TTCAGCCCAA AAATTCAGAC 
CCTTTTGCTC ATTCAAAGGA TGCATCAAGT ACTTCAAGTG GCCAATCAGG AAGCAAAAAT 
GAGGGTGATG AAGAGAGAGG GCTTATTCTG AGTGATGCTG TGTGGTCAAC AAAGGTGGAC 
TGTCTGAATC CCATTAATCA CCAGAGACTT TGTGTGCTCT TCAGCAGCTC TTCTGCCCAG 
TCCAGCAATG CTCCTAGTGC CTGTGTCAGT CCTTGGATTG TAACAATGGA ATTTTATGGA 
AAGAATGATC TTACATTAGG AATATTTTTA GAGAGATACT GTTTCAGGCC TTCTTATCAG 
TGTCCAAGCA TGTTCTGTGA TACCCCCATG GTACATCATA TTCGGCGCTT TGTTCATGGC 
CAAGGCTGTG TGCAGATAAT CCTGAAGGAG TTGGATTCTC CAGTACCTGG ATATCAGCAT 
ACAATTCTTA CATATTCCTG GTGTAGAATC TGCAAACAGT TATTCTCCCA TTCGGCTTCT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PIKFYVE ( XM_011510792.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011509094.1 Homo sapiens phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X21, mRNA.

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CloneID	OHu77673
Clone ID Related Accession (Same CDS sequence)	XM_011510792.3 , XM_011510792.2
Accession Version	XM_011510792.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4023bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	1-phosphatidylinositol 3-phosphate 5-kinase isoform X17
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011510792.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG ATGATAAGAC GTCCCCAACA CTGGACTCTG CTAATGATTT GCCTCGATCT 
CCTACTAGTC CTTCTCATCT CACACACTTT AAACCTTTGA CTCCTGATCA AGATGAGCCC 
CCTTTTAAAT CAGCTTATAG TTCTTTTGTA AATCTCTTTC GTTTTAACAA AGAGAGAGCA 
GAAGGAGGCC AGGGAGAACA GCAGCCTTTG AGTGGAAGTT GGACCAGCCC TCAGCTCCCT 
TCGAGGACAC AGTCTGTTAG GTCACCCACA CCTTATAAAA AGCAGCTTAA TGAGGAACTC 
CAGCGGCGCT CTTCAGCATT AGCAGACAAC AGTTTGCAAC ATCCCCAGGA GAACACAGAC 
ACAAGAAGGA AAGCAGAACC TACCTTTGGA GGTCATGACC CTCGTACAGC TGTTCAGCTT 
CGAAGCCTCA GCACAGTATT AAAACGCCTC AAGGAAATCA TGGAGGGGAA AAGCCAGGAT 
AGTGACCTGA AACAATACTG GATGCCAGAT AGCCAATGTA AAGAGTGCTA TGACTGTAGT 
GAGAAATTTA CAACCTTTAG GCGCAGACAC CATTGCCGAC TATGTGGGCA GATTTTCTGC 
AGTCGTTGCT GTAATCAAGA AATCCCTGGA AAATTTATGG GCTATACAGG AGACCTCCGA 
GCTTGCACAT ATTGTAGAAA AATAGCCTTA AGTTATGCTC ATTCCACAGA CAGTAATTCT 
ATTGGGGAAG ACTTGAATGC TCTTTCAGAT TCTGCTTGCT CTGTGTCTGT GCTTGATCCA 
AGTGAACCCC GAACACCTGT TGGGAGTAGG AAAGCCAGCC GTAACATATT TTTAGAGGAT 
GATTTGGCCT GGCAAAGTTT GATTCATCCA GATTCCTCAA ATACTCCTCT TTCAACAAGA 
CTTGTATCTG TGCAAGAGGA TGCTGGGAAA TCTCCTGCTC GAAATAGATC AGCCAGCATT 
ACTAACCTGT CACTGGATAG ATCTGGTTCT CCTATGGTAC CTTCATATGA GACATCTGTC 
AGTCCCCAGG CTAACCGAAC ATATGTTAGG ACAGAGACCA CTGAGGATGA ACGCAAAATT 
CTTCTGGACA GTGTGCAGTT AAAAGACCTG TGGAAAAAAA TCTGCCATCA CAGCAGTGGA 
ATGGAGTTTC AGGATCACCG CTACTGGTTG AGAACGCATC CCAACTGCAT TGTAGGAAAG 
GAATTAGTCA ACTGGCTAAT CCGAAATGGG CATATTGCCA CAAGGGCACA AGCTATAGCA 
ATTGGACAAG CAATGGTTGA TGGACGTTGG CTGGATTGTG TTAGTCATCA CGACCAGCTT 
TTCAGAGATG AGTATGCGCT GTATAGACCA CTGCAGAGTA CAGAATTTTC TGAGACGCCT 
TCTCCCGACA GTGACTCAGT GAACTCCGTG GAAGGACACT CTGAGCCATC CTGGTTTAAA 
GACATAAAGT TTGATGACAG TGACACAGAA CAGATAGCTG AAGAAGGTGA CGATAATTTG 
GCTAATTCTG CCAGTCCTAG CAAGCGCACA TCAGTCAGCA GTTTCCAGTC CACAGTGGAC 
AGTGACTCAG CCGCTTCTAT CAGCCTGAAC GTGGAGCTGG ACAACGTGAA CTTCCATATC 
AAGAAGCCCT CCAAGTACCC ACATGTGCCC CCTCACCCTG CTGACCAAAA AGAGTATTTG 
ATTTCTGACA CTGGAGGACA ACAGCTCTCA ATAAGTGACG CTTTCATCAA AGAATCCTTA 
TTTAATCGCC GAGTAGAGGA AAAATCCAAA GAGCTGCCTT TCACACCTTT GGGCTGGCAT 
CATAACAACC TGGAGCTCCT GAGGGAGGAG AATGGGGAGA AACAAGCCAT GGAGAGGTTG 
CTTTCAGCTA ATCATAACCA CATGATGGCA CTACTCCAGC AGTTGCTCCA TAGTGACTCA 
CTGTCATCAT CTTGGAGGGA CATCATCGTG TCATTGGTCT GCCAGGTTGT TCAGACAGTC 
CGACCTGATG TCAAGAACCA GGATGATGAC ATGGATATCC GTCAGTTTGT CCACATCAAA 
AAAATCCCAG GTGGAAAGAA GTTTGATTCT GTGGTTGTCA ATGGCTTTGT TTGTACCAAG 
AACATTGCAC ATAAAAAGAT GAGTTCTTGT ATTAAAAACC CCAAAATTCT TCTGTTGAAG 
TGTTCCATTG AGTATCTCTA CAGAGAAGAA ACTAAGTTTA CTTGCATTGA TCCTATTGTG 
CTTCAGGAAA GGGAATTCTT GAAGAATTAT GTCCAGCGAA TAGTTGATGT TCGACCCACC 
TTGGTTCTTG TTGAGAAAAC AGTGTCTCGG ATTGCCCAGG ACATGTTATT GGAACATGGC 
ATTACTTTGG TCATTAATGT AAAGTCACAA GTTTTGGAAC GAATCAGTCG AATGACCCAA 
GGTGATTTAG TGATGTCAAT GGACCAGCTG CTTACGAAAC CACACCTGGG CACTTGTCAC 
AAATTTTATA TGCAGATATT TCAGTTGCCT AATGAACAAA CCAAGACACT GATGTTTTTT 
GAAGGTTGTC CACAGCACCT AGGCTGTACA ATCAAGCTAA GAGGAGGCTC TGATTATGAG 
CTGGCTCGAG TTAAGGAGAT CCTAATATTT ATGATCTGTG TTGCTTATCA TTCTCAACTA 
GAAATATCCT TTCTCATGGA TGAATTTGCT ATGCCTCCCA CATTAATGCA AAACCCTTCA 
TTCCATTCCC TGATTGAGGG ACGAGGGCAT GAGGGGGCTG TCCAAGAGCA GTACGGTGGA 
GGTTCCATCC CCTGGGATCC TGACATCCCT CCTGAGTCTC TGCCCTGTGA TGATAGCAGT 
TTGCTGGAAT TGAGGATTGT GTTTGAGAAG GGTGAGCAGG AAAATAAAAA TCTTCCGCAG 
GCTGTTGCCT CTGTGAAGCA TCAAGAACAT AGCACAACAG CTTGCCCGGC GGGTCTCCCT 
TGTGCTTTCT TTGCACCTGT ACCGGAATCA TTGTTGCCAC TCCCTGTGGA TGACCAACAA 
GATGCTTTAG GCAGCGAGCA GCCAGAGACT TTGCAGCAAA CAGTTGTGCT GCAGGATCCC 
AAAAGCCAGA TAAGAGCCTT TAGAGACCCT CTACAGGATG ACACTGGATT ATATGTTACT 
GAGGAAGTCA CCTCCTCTGA AGATAAACGA AAGACTTATT CTTTGGCCTT TAAGCAGGAA 
TTAAAAGATG TGATCCTCTG TATCTCCCCA GTAATCACAT TCCGAGAACC CTTTCTTTTA 
ACTGAAAAGG GGATGAGATG CTCTACCCGA GATTATTTTG CAGAGCAGGT TTACTGGTCT 
CCTCTCCTCA ATAAAGAATT CAAAGAAATG GAGAACAGGA GGAAGAAACA GCTGCTCAGG 
GATCTCTCTG GACTTCAGGG CATGAATGGA AGTATTCAGG CCAAGTCTAT TCAAGTCTTA 
CCCTCACATG AGCTAGTGAG CACTAGAATT GCTGAGCATC TGGGCGATAG CCAGAGCTTG 
GGTAGAATGC TGGCCGATTA TCGAGCCAGA GGAGGAAGAA TTCAGCCCAA AAATTCAGAC 
CCTTTTGCTC ATTCAAAGGA TGCATCAAGT ACTTCAAGTG GCCAATCAGG AAGCAAAAAT 
GAGGGTGATG AAGAGAGAGG GCTTATTCTG AGTGATGCTG TGTGGTCAAC AAAGGTGGAC 
TGTCTGAATC CCATTAATCA CCAGAGACTT TGTGTGCTCT TCAGCAGCTC TTCTGCCCAG 
TCCAGCAATG CTCCTAGTGC CTGTGTCAGT CCTTGGATTG TAACAATGGA ATTTTATGGA 
AAGAATGATC TTACATTAGG AATATTTTTA GAGAGATACT GTTTCAGGCC TTCTTATCAG 
TGTCCAAGCA TGTTCTGTGA TACCCCCATG GTACATCATA TTCGGCGCTT TGTTCATGGC 
CAAGGCTGTG TGCAGATAAT CCTGAAGGAG TTGGATTCTC CAGTACCTGG ATATCAGCAT 
ACAATTCTTA CATATTCCTG GTGTAGAATC TGCAAACAGT TATTCTCCCA TTCGGCTTCT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011509094.1
CDS	173..4195
Translation	MATDDKTSPTLDSANDLPRSPTSPSHLTHFKPLTPDQDEPPFKSAYSSFVNLFRFNKERAEGGQGEQQPLSGSWTSPQLPSRTQSVRSPTPYKKQLNEELQRRSSALADNSLQHPQENTDTRRKAEPTFGGHDPRTAVQLRSLSTVLKRLKEIMEGKSQDSDLKQYWMPDSQCKECYDCSEKFTTFRRRHHCRLCGQIFCSRCCNQEIPGKFMGYTGDLRACTYCRKIALSYAHSTDSNSIGEDLNALSDSACSVSVLDPSEPRTPVGSRKASRNIFLEDDLAWQSLIHPDSSNTPLSTRLVSVQEDAGKSPARNRSASITNLSLDRSGSPMVPSYETSVSPQANRTYVRTETTEDERKILLDSVQLKDLWKKICHHSSGMEFQDHRYWLRTHPNCIVGKELVNWLIRNGHIATRAQAIAIGQAMVDGRWLDCVSHHDQLFRDEYALYRPLQSTEFSETPSPDSDSVNSVEGHSEPSWFKDIKFDDSDTEQIAEEGDDNLANSASPSKRTSVSSFQSTVDSDSAASISLNVELDNVNFHIKKPSKYPHVPPHPADQKEYLISDTGGQQLSISDAFIKESLFNRRVEEKSKELPFTPLGWHHNNLELLREENGEKQAMERLLSANHNHMMALLQQLLHSDSLSSSWRDIIVSLVCQVVQTVRPDVKNQDDDMDIRQFVHIKKIPGGKKFDSVVVNGFVCTKNIAHKKMSSCIKNPKILLLKCSIEYLYREETKFTCIDPIVLQEREFLKNYVQRIVDVRPTLVLVEKTVSRIAQDMLLEHGITLVINVKSQVLERISRMTQGDLVMSMDQLLTKPHLGTCHKFYMQIFQLPNEQTKTLMFFEGCPQHLGCTIKLRGGSDYELARVKEILIFMICVAYHSQLEISFLMDEFAMPPTLMQNPSFHSLIEGRGHEGAVQEQYGGGSIPWDPDIPPESLPCDDSSLLELRIVFEKGEQENKNLPQAVASVKHQEHSTTACPAGLPCAFFAPVPESLLPLPVDDQQDALGSEQPETLQQTVVLQDPKSQIRAFRDPLQDDTGLYVTEEVTSSEDKRKTYSLAFKQELKDVILCISPVITFREPFLLTEKGMRCSTRDYFAEQVYWSPLLNKEFKEMENRRKKQLLRDLSGLQGMNGSIQAKSIQVLPSHELVSTRIAEHLGDSQSLGRMLADYRARGGRIQPKNSDPFAHSKDASSTSSGQSGSKNEGDEERGLILSDAVWSTKVDCLNPINHQRLCVLFSSSSAQSSNAPSACVSPWIVTMEFYGKNDLTLGIFLERYCFRPSYQCPSMFCDTPMVHHIRRFVHGQGCVQIILKELDSPVPGYQHTILTYSWCRICKQLFSHSAS

Target ORF information:

RefSeq Version	XM_011510792.2
Organism	Homo sapiens(human)
Definition	Homo sapiens phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X21, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011510792.2

ORF Insert Sequence:

ATGGCCACAG ATGATAAGAC GTCCCCAACA CTGGACTCTG CTAATGATTT GCCTCGATCT 
CCTACTAGTC CTTCTCATCT CACACACTTT AAACCTTTGA CTCCTGATCA AGATGAGCCC 
CCTTTTAAAT CAGCTTATAG TTCTTTTGTA AATCTCTTTC GTTTTAACAA AGAGAGAGCA 
GAAGGAGGCC AGGGAGAACA GCAGCCTTTG AGTGGAAGTT GGACCAGCCC TCAGCTCCCT 
TCGAGGACAC AGTCTGTTAG GTCACCCACA CCTTATAAAA AGCAGCTTAA TGAGGAACTC 
CAGCGGCGCT CTTCAGCATT AGCAGACAAC AGTTTGCAAC ATCCCCAGGA GAACACAGAC 
ACAAGAAGGA AAGCAGAACC TACCTTTGGA GGTCATGACC CTCGTACAGC TGTTCAGCTT 
CGAAGCCTCA GCACAGTATT AAAACGCCTC AAGGAAATCA TGGAGGGGAA AAGCCAGGAT 
AGTGACCTGA AACAATACTG GATGCCAGAT AGCCAATGTA AAGAGTGCTA TGACTGTAGT 
GAGAAATTTA CAACCTTTAG GCGCAGACAC CATTGCCGAC TATGTGGGCA GATTTTCTGC 
AGTCGTTGCT GTAATCAAGA AATCCCTGGA AAATTTATGG GCTATACAGG AGACCTCCGA 
GCTTGCACAT ATTGTAGAAA AATAGCCTTA AGTTATGCTC ATTCCACAGA CAGTAATTCT 
ATTGGGGAAG ACTTGAATGC TCTTTCAGAT TCTGCTTGCT CTGTGTCTGT GCTTGATCCA 
AGTGAACCCC GAACACCTGT TGGGAGTAGG AAAGCCAGCC GTAACATATT TTTAGAGGAT 
GATTTGGCCT GGCAAAGTTT GATTCATCCA GATTCCTCAA ATACTCCTCT TTCAACAAGA 
CTTGTATCTG TGCAAGAGGA TGCTGGGAAA TCTCCTGCTC GAAATAGATC AGCCAGCATT 
ACTAACCTGT CACTGGATAG ATCTGGTTCT CCTATGGTAC CTTCATATGA GACATCTGTC 
AGTCCCCAGG CTAACCGAAC ATATGTTAGG ACAGAGACCA CTGAGGATGA ACGCAAAATT 
CTTCTGGACA GTGTGCAGTT AAAAGACCTG TGGAAAAAAA TCTGCCATCA CAGCAGTGGA 
ATGGAGTTTC AGGATCACCG CTACTGGTTG AGAACGCATC CCAACTGCAT TGTAGGAAAG 
GAATTAGTCA ACTGGCTAAT CCGAAATGGG CATATTGCCA CAAGGGCACA AGCTATAGCA 
ATTGGACAAG CAATGGTTGA TGGACGTTGG CTGGATTGTG TTAGTCATCA CGACCAGCTT 
TTCAGAGATG AGTATGCGCT GTATAGACCA CTGCAGAGTA CAGAATTTTC TGAGACGCCT 
TCTCCCGACA GTGACTCAGT GAACTCCGTG GAAGGACACT CTGAGCCATC CTGGTTTAAA 
GACATAAAGT TTGATGACAG TGACACAGAA CAGATAGCTG AAGAAGGTGA CGATAATTTG 
GCTAATTCTG CCAGTCCTAG CAAGCGCACA TCAGTCAGCA GTTTCCAGTC CACAGTGGAC 
AGTGACTCAG CCGCTTCTAT CAGCCTGAAC GTGGAGCTGG ACAACGTGAA CTTCCATATC 
AAGAAGCCCT CCAAGTACCC ACATGTGCCC CCTCACCCTG CTGACCAAAA AGAGTATTTG 
ATTTCTGACA CTGGAGGACA ACAGCTCTCA ATAAGTGACG CTTTCATCAA AGAATCCTTA 
TTTAATCGCC GAGTAGAGGA AAAATCCAAA GAGCTGCCTT TCACACCTTT GGGCTGGCAT 
CATAACAACC TGGAGCTCCT GAGGGAGGAG AATGGGGAGA AACAAGCCAT GGAGAGGTTG 
CTTTCAGCTA ATCATAACCA CATGATGGCA CTACTCCAGC AGTTGCTCCA TAGTGACTCA 
CTGTCATCAT CTTGGAGGGA CATCATCGTG TCATTGGTCT GCCAGGTTGT TCAGACAGTC 
CGACCTGATG TCAAGAACCA GGATGATGAC ATGGATATCC GTCAGTTTGT CCACATCAAA 
AAAATCCCAG GTGGAAAGAA GTTTGATTCT GTGGTTGTCA ATGGCTTTGT TTGTACCAAG 
AACATTGCAC ATAAAAAGAT GAGTTCTTGT ATTAAAAACC CCAAAATTCT TCTGTTGAAG 
TGTTCCATTG AGTATCTCTA CAGAGAAGAA ACTAAGTTTA CTTGCATTGA TCCTATTGTG 
CTTCAGGAAA GGGAATTCTT GAAGAATTAT GTCCAGCGAA TAGTTGATGT TCGACCCACC 
TTGGTTCTTG TTGAGAAAAC AGTGTCTCGG ATTGCCCAGG ACATGTTATT GGAACATGGC 
ATTACTTTGG TCATTAATGT AAAGTCACAA GTTTTGGAAC GAATCAGTCG AATGACCCAA 
GGTGATTTAG TGATGTCAAT GGACCAGCTG CTTACGAAAC CACACCTGGG CACTTGTCAC 
AAATTTTATA TGCAGATATT TCAGTTGCCT AATGAACAAA CCAAGACACT GATGTTTTTT 
GAAGGTTGTC CACAGCACCT AGGCTGTACA ATCAAGCTAA GAGGAGGCTC TGATTATGAG 
CTGGCTCGAG TTAAGGAGAT CCTAATATTT ATGATCTGTG TTGCTTATCA TTCTCAACTA 
GAAATATCCT TTCTCATGGA TGAATTTGCT ATGCCTCCCA CATTAATGCA AAACCCTTCA 
TTCCATTCCC TGATTGAGGG ACGAGGGCAT GAGGGGGCTG TCCAAGAGCA GTACGGTGGA 
GGTTCCATCC CCTGGGATCC TGACATCCCT CCTGAGTCTC TGCCCTGTGA TGATAGCAGT 
TTGCTGGAAT TGAGGATTGT GTTTGAGAAG GGTGAGCAGG AAAATAAAAA TCTTCCGCAG 
GCTGTTGCCT CTGTGAAGCA TCAAGAACAT AGCACAACAG CTTGCCCGGC GGGTCTCCCT 
TGTGCTTTCT TTGCACCTGT ACCGGAATCA TTGTTGCCAC TCCCTGTGGA TGACCAACAA 
GATGCTTTAG GCAGCGAGCA GCCAGAGACT TTGCAGCAAA CAGTTGTGCT GCAGGATCCC 
AAAAGCCAGA TAAGAGCCTT TAGAGACCCT CTACAGGATG ACACTGGATT ATATGTTACT 
GAGGAAGTCA CCTCCTCTGA AGATAAACGA AAGACTTATT CTTTGGCCTT TAAGCAGGAA 
TTAAAAGATG TGATCCTCTG TATCTCCCCA GTAATCACAT TCCGAGAACC CTTTCTTTTA 
ACTGAAAAGG GGATGAGATG CTCTACCCGA GATTATTTTG CAGAGCAGGT TTACTGGTCT 
CCTCTCCTCA ATAAAGAATT CAAAGAAATG GAGAACAGGA GGAAGAAACA GCTGCTCAGG 
GATCTCTCTG GACTTCAGGG CATGAATGGA AGTATTCAGG CCAAGTCTAT TCAAGTCTTA 
CCCTCACATG AGCTAGTGAG CACTAGAATT GCTGAGCATC TGGGCGATAG CCAGAGCTTG 
GGTAGAATGC TGGCCGATTA TCGAGCCAGA GGAGGAAGAA TTCAGCCCAA AAATTCAGAC 
CCTTTTGCTC ATTCAAAGGA TGCATCAAGT ACTTCAAGTG GCCAATCAGG AAGCAAAAAT 
GAGGGTGATG AAGAGAGAGG GCTTATTCTG AGTGATGCTG TGTGGTCAAC AAAGGTGGAC 
TGTCTGAATC CCATTAATCA CCAGAGACTT TGTGTGCTCT TCAGCAGCTC TTCTGCCCAG 
TCCAGCAATG CTCCTAGTGC CTGTGTCAGT CCTTGGATTG TAACAATGGA ATTTTATGGA 
AAGAATGATC TTACATTAGG AATATTTTTA GAGAGATACT GTTTCAGGCC TTCTTATCAG 
TGTCCAAGCA TGTTCTGTGA TACCCCCATG GTACATCATA TTCGGCGCTT TGTTCATGGC 
CAAGGCTGTG TGCAGATAAT CCTGAAGGAG TTGGATTCTC CAGTACCTGG ATATCAGCAT 
ACAATTCTTA CATATTCCTG GTGTAGAATC TGCAAACAGT TATTCTCCCA TTCGGCTTCT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PIKFYVE ( XM_017003568.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016859057.1 Homo sapiens phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X5, mRNA.

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CloneID	OHu89143
Clone ID Related Accession (Same CDS sequence)	XM_017003568.1
Accession Version	XM_017003568.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6279bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	1-phosphatidylinositol 3-phosphate 5-kinase isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG ATGATAAGAC GTCCCCAACA CTGGACTCTG CTAATGATTT GCCTCGATCT 
CCTACTAGTC CTTCTCATCT CACACACTTT AAACCTTTGA CTCCTGATCA AGATGAGCCC 
CCTTTTAAAT CAGCTTATAG TTCTTTTGTA AATCTCTTTC GTTTTAACAA AGAGAGAGCA 
GAAGGAGGCC AGGGAGAACA GCAGCCTTTG AGTGGAAGTT GGACCAGCCC TCAGCTCCCT 
TCGAGGACAC AGTCTGTTAG GTCACCCACA CCTTATAAAA AGCAGCTTAA TGAGGAACTC 
CAGCGGCGCT CTTCAGCATT AGACACAAGA AGGAAAGCAG AACCTACCTT TGGAGGTCAT 
GACCCTCGTA CAGCTGTTCA GCTTCGAAGC CTCAGCACAG TATTAAAACG CCTCAAGGAA 
ATCATGGAGG GGAAAAGCCA GGATAGTGAC CTGAAACAAT ACTGGATGCC AGATAGCCAA 
TGTAAAGAGT GCTATGACTG TAGTGAGAAA TTTACAACCT TTAGGCGCAG ACACCATTGC 
CGACTATGTG GGCAGATTTT CTGCAGTCGT TGCTGTAATC AAGAAATCCC TGGAAAATTT 
ATGGGCTATA CAGGAGACCT CCGAGCTTGC ACATATTGTA GAAAAATAGC CTTAAGTTAT 
GCTCATTCCA CAGACAGTAA TTCTATTGGG GAAGACTTGA ATGCTCTTTC AGATTCTGCT 
TGCTCTGTGT CTGTGCTTGA TCCAAGTGAA CCCCGAACAC CTGTTGGGAG TAGGAAAGCC 
AGCCGTAACA TATTTTTAGA GGATGATTTG GCCTGGCAAA GTTTGATTCA TCCAGATTCC 
TCAAATACTC CTCTTTCAAC AAGACTTGTA TCTGTGCAAG AGGATGCTGG GAAATCTCCT 
GCTCGAAATA GATCAGCCAG CATTACTAAC CTGTCACTGG ATAGATCTGG TTCTCCTATG 
GTACCTTCAT ATGAGACATC TGTCAGTCCC CAGGCTAACC GAACATATGT TAGGACAGAG 
ACCACTGAGG ATGAACGCAA AATTCTTCTG GACAGTGTGC AGTTAAAAGA CCTGTGGAAA 
AAAATCTGCC ATCACAGCAG TGGAATGGAG TTTCAGGATC ACCGCTACTG GTTGAGAACG 
CATCCCAACT GCATTGTAGG AAAGGAATTA GTCAACTGGC TAATCCGAAA TGGGCATATT 
GCCACAAGGG CACAAGCTAT AGCAATTGGA CAAGCAATGG TTGATGGACG TTGGCTGGAT 
TGTGTTAGTC ATCACGACCA GCTTTTCAGA GATGAGTATG CGCTGTATAG ACCACTGCAG 
AGTACAGAAT TTTCTGAGAC GCCTTCTCCC GACAGTGACT CAGTGAACTC CGTGGAAGGA 
CACTCTGAGC CATCCTGGTT TAAAGACATA AAGTTTGATG ACAGTGACAC AGAACAGATA 
GCTGAAGAAG ATTCTGCCAG TCCTAGCAAG CGCACATCAG TCAGCAGTTT CCAGTCCACA 
GTGGACAGTG ACTCAGCCGC TTCTATCAGC CTGAACGTGG AGCTGGACAA CGTGAACTTC 
CATATCAAGA AGCCCTCCAA GTACCCACAT GTGCCCCCTC ACCCTGCTGA CCAAAAAGAG 
TATTTGATTT CTGACACTGG AGGACAACAG CTCTCAATAA GTGACGCTTT CATCAAAGAA 
TCCTTATTTA ATCGCCGAGT AGAGGAAAAA TCCAAAGAGC TGCCTTTCAC ACCTTTGGGC 
TGGCATCATA ACAACCTGGA GCTCCTGAGG GAGGAGAATG GGGAGAAACA AGCCATGGAG 
AGGTTGCTTT CAGCTAATCA TAACCACATG ATGGCACTAC TCCAGCAGTT GCTCCATAGT 
GACTCACTGT CATCATCTTG GAGGGACATC ATCGTGTCAT TGGTCTGCCA GGTTGTTCAG 
ACAGTCCGAC CTGATGTCAA GAACCAGGAT GATGACATGG ATATCCGTCA GTTTGTCCAC 
ATCAAAAAAA TCCCAGGTGG AAAGAAGTTT GATTCTGTGG TTGTCAATGG CTTTGTTTGT 
ACCAAGAACA TTGCACATAA AAAGATGAGT TCTTGTATTA AAAACCCCAA AATTCTTCTG 
TTGAAGTGTT CCATTGAGTA TCTCTACAGA GAAGAAACTA AGTTTACTTG CATTGATCCT 
ATTGTGCTTC AGGAAAGGGA ATTCTTGAAG AATTATGTCC AGCGAATAGT TGATGTTCGA 
CCCACCTTGG TTCTTGTTGA GAAAACAGTG TCTCGGATTG CCCAGGACAT GTTATTGGAA 
CATGGCATTA CTTTGGTCAT TAATGTAAAG TCACAAGTTT TGGAACGAAT CAGTCGAATG 
ACCCAAGGTG ATTTAGTGAT GTCAATGGAC CAGCTGCTTA CGAAACCACA CCTGGGCACT 
TGTCACAAAT TTTATATGCA GATATTTCAG TTGCCTAATG AACAAACCAA GACACTGATG 
TTTTTTGAAG GTTGTCCACA GCACCTAGGC TGTACAATCA AGCTAAGAGG AGGCTCTGAT 
TATGAGCTGG CTCGAGTTAA GGAGATCCTA ATATTTATGA TCTGTGTTGC TTATCATTCT 
CAACTAGAAA TATCCTTTCT CATGGATGAA TTTGCTATGC CTCCCACATT AATGCAAAAC 
CCTTCATTCC ATTCCCTGAT TGAGGGACGA GGGCATGAGG GGGCTGTCCA AGAGCAGTAC 
GGTGGAGGTT CCATCCCCTG GGATCCTGAC ATCCCTCCTG AGTCTCTGCC CTGTGATGAT 
AGCAGTTTGC TGGAATTGAG GATTGTGTTT GAGAAGGGTG AGCAGGAAAA TAAAAATCTT 
CCGCAGGCTG TTGCCTCTGT GAAGCATCAA GAACATAGCA CAACAGCTTG CCCGGCGGGT 
CTCCCTTGTG CTTTCTTTGC ACCTGTACCG GAATCATTGT TGCCACTCCC TGTGGATGAC 
CAACAAGATG CTTTAGGCAG CGAGCAGCCA GAGACTTTGC AGCAAACAGT TGTGCTGCAG 
GATCCCAAAA GCCAGATAAG AGCCTTTAGA GACCCTCTAC AGGATGACAC TGGATTATAT 
GTTACTGAGG AAGTCACCTC CTCTGAAGAT AAACGAAAGA CTTATTCTTT GGCCTTTAAG 
CAGGAATTAA AAGATGTGAT CCTCTGTATC TCCCCAGTAA TCACATTCCG AGAACCCTTT 
CTTTTAACTG AAAAGGGGAT GAGATGCTCT ACCCGAGATT ATTTTGCAGA GCAGGTTTAC 
TGGTCTCCTC TCCTCAATAA AGAATTCAAA GAAATGGAGA ACAGGAGGAA GAAACAGCTG 
CTCAGGGATC TCTCTGGACT TCAGGGCATG AATGGAAGTA TTCAGGCCAA GTCTATTCAA 
GTCTTACCCT CACATGAGCT AGTGAGCACT AGAATTGCTG AGCATCTGGG CGATAGCCAG 
AGCTTGGGTA GAATGCTGGC CGATTATCGA GCCAGAGGAG GAAGAATTCA GCCCAAAAAT 
TCAGACCCTT TTGCTCATTC AAAGGATGCA TCAAGTACTT CAAGTGGCCA ATCAGGAAGC 
AAAAATGAGG GTGATGAAGA GAGAGGGCTT ATTCTGAGTG ATGCTGTGTG GTCAACAAAG 
GTGGACTGTC TGAATCCCAT TAATCACCAG AGACTTTGTG TGCTCTTCAG CAGCTCTTCT 
GCCCAGTCCA GCAATGCTCC TAGTGCCTGT GTCAGTCCTT GGATTGTAAC AATGGAATTT 
TATGGAAAGA ATGATCTTAC ATTAGGAATA TTTTTAGAGA GATACTGTTT CAGGCCTTCT 
TATCAGTGTC CAAGCATGTT CTGTGATACC CCCATGGTAC ATCATATTCG GCGCTTTGTT 
CATGGCCAAG GCTGTGTGCA GATAATCCTG AAGGAGTTGG ATTCTCCAGT ACCTGGATAT 
CAGCATACAA TTCTTACATA TTCCTGGTGT AGAATCTGCA AACAGGTAAC ACCAGTTGTT 
GCTCTTTCCA ATGAGTCCTG GTCTATGTCA TTTGCAAAAT ACCTTGAACT TAGGTTTTAT 
GGGCACCAGT ATACTCGCAG AGCCAACGCT GAGCCCTGTG GTCACTCCAT CCATCATGAT 
TATCACCAGT ATTTCTCCTA TAACCAGATG GTGGCGTCTT TCAGTTATTC TCCCATTCGG 
CTTCTTGAAG TATGTGTTCC ACTCCCCAAA ATATTCATTA AGCGTCAGGC CCCATTAAAA 
GTGTCCCTTC TTCAGGATCT GAAGGACTTC TTTCAAAAAG TTTCACAGGT ATATGTTGCC 
ATTGATGAAA GACTTGCATC TTTGAAAACT GATACATTTA GTAAAACAAG AGAGGAAAAA 
ATGGAAGATA TTTTTGCACA GAAAGAGATG GAAGAAGGTG AGTTCAAGAA CTGGATTGAG 
AAGATGCAAG CAAGGCTCAT GTCTTCCTCT GTAGATACCC CTCAGCAACT GCAGTCGGTC 
TTTGAGTCAC TCATTGCCAA GAAACAAAGT CTCTGTGAAG TGCTGCAAGC TTGGAATAAC 
AGGTTGCAGG ACCTTTTCCA ACAGGAAAAG GGTAGAAAGA GACCTTCAGT TCCTCCAAGT 
CCTGGAAGAC TGAGACAAGG GGAAGAAAGC AAGATAAGTG CGATGGATGC ATCTCCACGG 
AATATTTCTC CAGGACTTCA GAATGGAGAA AAAGAGGATC GCTTCTTAAC AACTTTGTCC 
AGCCAGAGCT CCACCAGTTC TACTCATCTC CAATTGCCTA CGCCACCTGA AGTCATGTCT 
GAACAGTCAG TGGGAGGGCC CCCTGAGCTA GATACAGCCA GCAGTTCCGA AGATGTGTTT 
GATGGGCATT TGCTGGGATC CACAGACAGC CAAGTGAAGG AAAAGTCAAC CATGAAAGCC 
ATCTTTGCAA ATTTGCTTCC AGGAAATAGC TATAATCCTA TTCCATTTCC TTTTGATCCA 
GATAAACACT ACTTAATGTA TGAACATGAA CGAGTGCCCA TTGCAGTCTG CGAGAAGGAA 
CCCAGCTCCA TCATTGCTTT TGCTCTCAGT TGTAAAGAAT ACCGAAATGC CTTAGAGGAA 
TTGTCTAAAG CGACTCAGTG GAACAGTGCC GAAGAAGGGC TTCCAACAAA TAGTACTTCA 
GATAGCAGAC CAAAGAGTAG CAGCCCTATC AGATTACCTG AAATGAGTGG AGGACAGACA 
AATCGTACAA CAGAAACAGA ACCACAACCA ACCAAAAAGG CTTCTGGAAT GTTGTCCTTC 
TTCAGAGGGA CAGCAGGGAA AAGCCCCGAT CTCTCTTCCC AGAAGAGAGA GACCTTACGT 
GGAGCAGATA GTGCTTACTA CCAGGTTGGG CAGACGGGCA AGGAGGGGAC CGAGAATCAA 
GGCGTTGAGC CTCAAGATGA AGTAGATGGA GGAGATACAC AAAAGAAGCA ACTCATAAAT 
CCTCATGTGG AACTTCAATT TTCAGATGCT AATGCCAAGT TTTACTGTCG GCTCTACTAT 
GCGGGAGAGT TTCATAAGAT GCGTGAAGTG ATTCTGGACA GCAGTGAAGA AGATTTCATT 
CGTTCCCTCT CCCACTCATC ACCCTGGCAG GCCCGGGGAG GCAAATCAGG AGCTGCCTTC 
TATGCAACTG AGGATGATAG ATTTATTTTG AAGCAAATGC CTCGTCTGGA AGTCCAGTCC 
TTCCTCGACT TTGCACCACA TTACTTCAAT TATATTACAA ATGCTGTTCA ACAAAAGAGG 
CCCACGGCGT TGGCCAAAAT TCTTGGAGTT TACAGAATTG GTTATAAGAA CTCTCAGAAC 
AACACTGAGA AGAAGTTAGA TCTCCTTGTC ATGGAAAATC TTTTCTACGG GAGAAAGATG 
GCACAGGTTT TTGATTTGAA GGGCTCTCTT AGGAATCGGA ATGTAAAAAC TGACACTGGA 
AAAGAGAGTT GTGATGTGGT CCTGCTAGAT GAAAATCTCC TAAAGATGGT TCGAGACAAC 
CCTCTATATA TTCGTTCTCA TTCCAAAGCT GTGCTGAGAA CCTCGATCCA TAGTGACTCC 
CATTTCCTTT CTAGCCACCT CATTATAGAT TATTCTTTGC TGGTTGGGCG AGATGATACT 
AGCAATGAGC TAGTAGTTGG AATTATAGAT TATATTCGAA CATTTACATG GGACAAAAAG 
CTTGAGATGG TTGTGAAATC AACAGGAATT TTAGGTGGAC AAGGTAAAAT GCCAACAGTG 
GTGTCTCCGG AGTTGTACAG GACTAGGTTT TGTGAGGCAA TGGACAAGTA TTTCCTAATG 
GTACCAGACC ACTGGACAGG CTTGGGTCTG AATTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016859057.1
CDS	63..6341
Translation	MATDDKTSPTLDSANDLPRSPTSPSHLTHFKPLTPDQDEPPFKSAYSSFVNLFRFNKERAEGGQGEQQPLSGSWTSPQLPSRTQSVRSPTPYKKQLNEELQRRSSALDTRRKAEPTFGGHDPRTAVQLRSLSTVLKRLKEIMEGKSQDSDLKQYWMPDSQCKECYDCSEKFTTFRRRHHCRLCGQIFCSRCCNQEIPGKFMGYTGDLRACTYCRKIALSYAHSTDSNSIGEDLNALSDSACSVSVLDPSEPRTPVGSRKASRNIFLEDDLAWQSLIHPDSSNTPLSTRLVSVQEDAGKSPARNRSASITNLSLDRSGSPMVPSYETSVSPQANRTYVRTETTEDERKILLDSVQLKDLWKKICHHSSGMEFQDHRYWLRTHPNCIVGKELVNWLIRNGHIATRAQAIAIGQAMVDGRWLDCVSHHDQLFRDEYALYRPLQSTEFSETPSPDSDSVNSVEGHSEPSWFKDIKFDDSDTEQIAEEDSASPSKRTSVSSFQSTVDSDSAASISLNVELDNVNFHIKKPSKYPHVPPHPADQKEYLISDTGGQQLSISDAFIKESLFNRRVEEKSKELPFTPLGWHHNNLELLREENGEKQAMERLLSANHNHMMALLQQLLHSDSLSSSWRDIIVSLVCQVVQTVRPDVKNQDDDMDIRQFVHIKKIPGGKKFDSVVVNGFVCTKNIAHKKMSSCIKNPKILLLKCSIEYLYREETKFTCIDPIVLQEREFLKNYVQRIVDVRPTLVLVEKTVSRIAQDMLLEHGITLVINVKSQVLERISRMTQGDLVMSMDQLLTKPHLGTCHKFYMQIFQLPNEQTKTLMFFEGCPQHLGCTIKLRGGSDYELARVKEILIFMICVAYHSQLEISFLMDEFAMPPTLMQNPSFHSLIEGRGHEGAVQEQYGGGSIPWDPDIPPESLPCDDSSLLELRIVFEKGEQENKNLPQAVASVKHQEHSTTACPAGLPCAFFAPVPESLLPLPVDDQQDALGSEQPETLQQTVVLQDPKSQIRAFRDPLQDDTGLYVTEEVTSSEDKRKTYSLAFKQELKDVILCISPVITFREPFLLTEKGMRCSTRDYFAEQVYWSPLLNKEFKEMENRRKKQLLRDLSGLQGMNGSIQAKSIQVLPSHELVSTRIAEHLGDSQSLGRMLADYRARGGRIQPKNSDPFAHSKDASSTSSGQSGSKNEGDEERGLILSDAVWSTKVDCLNPINHQRLCVLFSSSSAQSSNAPSACVSPWIVTMEFYGKNDLTLGIFLERYCFRPSYQCPSMFCDTPMVHHIRRFVHGQGCVQIILKELDSPVPGYQHTILTYSWCRICKQVTPVVALSNESWSMSFAKYLELRFYGHQYTRRANAEPCGHSIHHDYHQYFSYNQMVASFSYSPIRLLEVCVPLPKIFIKRQAPLKVSLLQDLKDFFQKVSQVYVAIDERLASLKTDTFSKTREEKMEDIFAQKEMEEGEFKNWIEKMQARLMSSSVDTPQQLQSVFESLIAKKQSLCEVLQAWNNRLQDLFQQEKGRKRPSVPPSPGRLRQGEESKISAMDASPRNISPGLQNGEKEDRFLTTLSSQSSTSSTHLQLPTPPEVMSEQSVGGPPELDTASSSEDVFDGHLLGSTDSQVKEKSTMKAIFANLLPGNSYNPIPFPFDPDKHYLMYEHERVPIAVCEKEPSSIIAFALSCKEYRNALEELSKATQWNSAEEGLPTNSTSDSRPKSSSPIRLPEMSGGQTNRTTETEPQPTKKASGMLSFFRGTAGKSPDLSSQKRETLRGADSAYYQVGQTGKEGTENQGVEPQDEVDGGDTQKKQLINPHVELQFSDANAKFYCRLYYAGEFHKMREVILDSSEEDFIRSLSHSSPWQARGGKSGAAFYATEDDRFILKQMPRLEVQSFLDFAPHYFNYITNAVQQKRPTALAKILGVYRIGYKNSQNNTEKKLDLLVMENLFYGRKMAQVFDLKGSLRNRNVKTDTGKESCDVVLLDENLLKMVRDNPLYIRSHSKAVLRTSIHSDSHFLSSHLIIDYSLLVGRDDTSNELVVGIIDYIRTFTWDKKLEMVVKSTGILGGQGKMPTVVSPELYRTRFCEAMDKYFLMVPDHWTGLGLNC

Target ORF information:

RefSeq Version	XM_017003568.1
Organism	Homo sapiens(human)
Definition	Homo sapiens phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017003568.1

ORF Insert Sequence:

ATGGCCACAG ATGATAAGAC GTCCCCAACA CTGGACTCTG CTAATGATTT GCCTCGATCT 
CCTACTAGTC CTTCTCATCT CACACACTTT AAACCTTTGA CTCCTGATCA AGATGAGCCC 
CCTTTTAAAT CAGCTTATAG TTCTTTTGTA AATCTCTTTC GTTTTAACAA AGAGAGAGCA 
GAAGGAGGCC AGGGAGAACA GCAGCCTTTG AGTGGAAGTT GGACCAGCCC TCAGCTCCCT 
TCGAGGACAC AGTCTGTTAG GTCACCCACA CCTTATAAAA AGCAGCTTAA TGAGGAACTC 
CAGCGGCGCT CTTCAGCATT AGACACAAGA AGGAAAGCAG AACCTACCTT TGGAGGTCAT 
GACCCTCGTA CAGCTGTTCA GCTTCGAAGC CTCAGCACAG TATTAAAACG CCTCAAGGAA 
ATCATGGAGG GGAAAAGCCA GGATAGTGAC CTGAAACAAT ACTGGATGCC AGATAGCCAA 
TGTAAAGAGT GCTATGACTG TAGTGAGAAA TTTACAACCT TTAGGCGCAG ACACCATTGC 
CGACTATGTG GGCAGATTTT CTGCAGTCGT TGCTGTAATC AAGAAATCCC TGGAAAATTT 
ATGGGCTATA CAGGAGACCT CCGAGCTTGC ACATATTGTA GAAAAATAGC CTTAAGTTAT 
GCTCATTCCA CAGACAGTAA TTCTATTGGG GAAGACTTGA ATGCTCTTTC AGATTCTGCT 
TGCTCTGTGT CTGTGCTTGA TCCAAGTGAA CCCCGAACAC CTGTTGGGAG TAGGAAAGCC 
AGCCGTAACA TATTTTTAGA GGATGATTTG GCCTGGCAAA GTTTGATTCA TCCAGATTCC 
TCAAATACTC CTCTTTCAAC AAGACTTGTA TCTGTGCAAG AGGATGCTGG GAAATCTCCT 
GCTCGAAATA GATCAGCCAG CATTACTAAC CTGTCACTGG ATAGATCTGG TTCTCCTATG 
GTACCTTCAT ATGAGACATC TGTCAGTCCC CAGGCTAACC GAACATATGT TAGGACAGAG 
ACCACTGAGG ATGAACGCAA AATTCTTCTG GACAGTGTGC AGTTAAAAGA CCTGTGGAAA 
AAAATCTGCC ATCACAGCAG TGGAATGGAG TTTCAGGATC ACCGCTACTG GTTGAGAACG 
CATCCCAACT GCATTGTAGG AAAGGAATTA GTCAACTGGC TAATCCGAAA TGGGCATATT 
GCCACAAGGG CACAAGCTAT AGCAATTGGA CAAGCAATGG TTGATGGACG TTGGCTGGAT 
TGTGTTAGTC ATCACGACCA GCTTTTCAGA GATGAGTATG CGCTGTATAG ACCACTGCAG 
AGTACAGAAT TTTCTGAGAC GCCTTCTCCC GACAGTGACT CAGTGAACTC CGTGGAAGGA 
CACTCTGAGC CATCCTGGTT TAAAGACATA AAGTTTGATG ACAGTGACAC AGAACAGATA 
GCTGAAGAAG ATTCTGCCAG TCCTAGCAAG CGCACATCAG TCAGCAGTTT CCAGTCCACA 
GTGGACAGTG ACTCAGCCGC TTCTATCAGC CTGAACGTGG AGCTGGACAA CGTGAACTTC 
CATATCAAGA AGCCCTCCAA GTACCCACAT GTGCCCCCTC ACCCTGCTGA CCAAAAAGAG 
TATTTGATTT CTGACACTGG AGGACAACAG CTCTCAATAA GTGACGCTTT CATCAAAGAA 
TCCTTATTTA ATCGCCGAGT AGAGGAAAAA TCCAAAGAGC TGCCTTTCAC ACCTTTGGGC 
TGGCATCATA ACAACCTGGA GCTCCTGAGG GAGGAGAATG GGGAGAAACA AGCCATGGAG 
AGGTTGCTTT CAGCTAATCA TAACCACATG ATGGCACTAC TCCAGCAGTT GCTCCATAGT 
GACTCACTGT CATCATCTTG GAGGGACATC ATCGTGTCAT TGGTCTGCCA GGTTGTTCAG 
ACAGTCCGAC CTGATGTCAA GAACCAGGAT GATGACATGG ATATCCGTCA GTTTGTCCAC 
ATCAAAAAAA TCCCAGGTGG AAAGAAGTTT GATTCTGTGG TTGTCAATGG CTTTGTTTGT 
ACCAAGAACA TTGCACATAA AAAGATGAGT TCTTGTATTA AAAACCCCAA AATTCTTCTG 
TTGAAGTGTT CCATTGAGTA TCTCTACAGA GAAGAAACTA AGTTTACTTG CATTGATCCT 
ATTGTGCTTC AGGAAAGGGA ATTCTTGAAG AATTATGTCC AGCGAATAGT TGATGTTCGA 
CCCACCTTGG TTCTTGTTGA GAAAACAGTG TCTCGGATTG CCCAGGACAT GTTATTGGAA 
CATGGCATTA CTTTGGTCAT TAATGTAAAG TCACAAGTTT TGGAACGAAT CAGTCGAATG 
ACCCAAGGTG ATTTAGTGAT GTCAATGGAC CAGCTGCTTA CGAAACCACA CCTGGGCACT 
TGTCACAAAT TTTATATGCA GATATTTCAG TTGCCTAATG AACAAACCAA GACACTGATG 
TTTTTTGAAG GTTGTCCACA GCACCTAGGC TGTACAATCA AGCTAAGAGG AGGCTCTGAT 
TATGAGCTGG CTCGAGTTAA GGAGATCCTA ATATTTATGA TCTGTGTTGC TTATCATTCT 
CAACTAGAAA TATCCTTTCT CATGGATGAA TTTGCTATGC CTCCCACATT AATGCAAAAC 
CCTTCATTCC ATTCCCTGAT TGAGGGACGA GGGCATGAGG GGGCTGTCCA AGAGCAGTAC 
GGTGGAGGTT CCATCCCCTG GGATCCTGAC ATCCCTCCTG AGTCTCTGCC CTGTGATGAT 
AGCAGTTTGC TGGAATTGAG GATTGTGTTT GAGAAGGGTG AGCAGGAAAA TAAAAATCTT 
CCGCAGGCTG TTGCCTCTGT GAAGCATCAA GAACATAGCA CAACAGCTTG CCCGGCGGGT 
CTCCCTTGTG CTTTCTTTGC ACCTGTACCG GAATCATTGT TGCCACTCCC TGTGGATGAC 
CAACAAGATG CTTTAGGCAG CGAGCAGCCA GAGACTTTGC AGCAAACAGT TGTGCTGCAG 
GATCCCAAAA GCCAGATAAG AGCCTTTAGA GACCCTCTAC AGGATGACAC TGGATTATAT 
GTTACTGAGG AAGTCACCTC CTCTGAAGAT AAACGAAAGA CTTATTCTTT GGCCTTTAAG 
CAGGAATTAA AAGATGTGAT CCTCTGTATC TCCCCAGTAA TCACATTCCG AGAACCCTTT 
CTTTTAACTG AAAAGGGGAT GAGATGCTCT ACCCGAGATT ATTTTGCAGA GCAGGTTTAC 
TGGTCTCCTC TCCTCAATAA AGAATTCAAA GAAATGGAGA ACAGGAGGAA GAAACAGCTG 
CTCAGGGATC TCTCTGGACT TCAGGGCATG AATGGAAGTA TTCAGGCCAA GTCTATTCAA 
GTCTTACCCT CACATGAGCT AGTGAGCACT AGAATTGCTG AGCATCTGGG CGATAGCCAG 
AGCTTGGGTA GAATGCTGGC CGATTATCGA GCCAGAGGAG GAAGAATTCA GCCCAAAAAT 
TCAGACCCTT TTGCTCATTC AAAGGATGCA TCAAGTACTT CAAGTGGCCA ATCAGGAAGC 
AAAAATGAGG GTGATGAAGA GAGAGGGCTT ATTCTGAGTG ATGCTGTGTG GTCAACAAAG 
GTGGACTGTC TGAATCCCAT TAATCACCAG AGACTTTGTG TGCTCTTCAG CAGCTCTTCT 
GCCCAGTCCA GCAATGCTCC TAGTGCCTGT GTCAGTCCTT GGATTGTAAC AATGGAATTT 
TATGGAAAGA ATGATCTTAC ATTAGGAATA TTTTTAGAGA GATACTGTTT CAGGCCTTCT 
TATCAGTGTC CAAGCATGTT CTGTGATACC CCCATGGTAC ATCATATTCG GCGCTTTGTT 
CATGGCCAAG GCTGTGTGCA GATAATCCTG AAGGAGTTGG ATTCTCCAGT ACCTGGATAT 
CAGCATACAA TTCTTACATA TTCCTGGTGT AGAATCTGCA AACAGGTAAC ACCAGTTGTT 
GCTCTTTCCA ATGAGTCCTG GTCTATGTCA TTTGCAAAAT ACCTTGAACT TAGGTTTTAT 
GGGCACCAGT ATACTCGCAG AGCCAACGCT GAGCCCTGTG GTCACTCCAT CCATCATGAT 
TATCACCAGT ATTTCTCCTA TAACCAGATG GTGGCGTCTT TCAGTTATTC TCCCATTCGG 
CTTCTTGAAG TATGTGTTCC ACTCCCCAAA ATATTCATTA AGCGTCAGGC CCCATTAAAA 
GTGTCCCTTC TTCAGGATCT GAAGGACTTC TTTCAAAAAG TTTCACAGGT ATATGTTGCC 
ATTGATGAAA GACTTGCATC TTTGAAAACT GATACATTTA GTAAAACAAG AGAGGAAAAA 
ATGGAAGATA TTTTTGCACA GAAAGAGATG GAAGAAGGTG AGTTCAAGAA CTGGATTGAG 
AAGATGCAAG CAAGGCTCAT GTCTTCCTCT GTAGATACCC CTCAGCAACT GCAGTCGGTC 
TTTGAGTCAC TCATTGCCAA GAAACAAAGT CTCTGTGAAG TGCTGCAAGC TTGGAATAAC 
AGGTTGCAGG ACCTTTTCCA ACAGGAAAAG GGTAGAAAGA GACCTTCAGT TCCTCCAAGT 
CCTGGAAGAC TGAGACAAGG GGAAGAAAGC AAGATAAGTG CGATGGATGC ATCTCCACGG 
AATATTTCTC CAGGACTTCA GAATGGAGAA AAAGAGGATC GCTTCTTAAC AACTTTGTCC 
AGCCAGAGCT CCACCAGTTC TACTCATCTC CAATTGCCTA CGCCACCTGA AGTCATGTCT 
GAACAGTCAG TGGGAGGGCC CCCTGAGCTA GATACAGCCA GCAGTTCCGA AGATGTGTTT 
GATGGGCATT TGCTGGGATC CACAGACAGC CAAGTGAAGG AAAAGTCAAC CATGAAAGCC 
ATCTTTGCAA ATTTGCTTCC AGGAAATAGC TATAATCCTA TTCCATTTCC TTTTGATCCA 
GATAAACACT ACTTAATGTA TGAACATGAA CGAGTGCCCA TTGCAGTCTG CGAGAAGGAA 
CCCAGCTCCA TCATTGCTTT TGCTCTCAGT TGTAAAGAAT ACCGAAATGC CTTAGAGGAA 
TTGTCTAAAG CGACTCAGTG GAACAGTGCC GAAGAAGGGC TTCCAACAAA TAGTACTTCA 
GATAGCAGAC CAAAGAGTAG CAGCCCTATC AGATTACCTG AAATGAGTGG AGGACAGACA 
AATCGTACAA CAGAAACAGA ACCACAACCA ACCAAAAAGG CTTCTGGAAT GTTGTCCTTC 
TTCAGAGGGA CAGCAGGGAA AAGCCCCGAT CTCTCTTCCC AGAAGAGAGA GACCTTACGT 
GGAGCAGATA GTGCTTACTA CCAGGTTGGG CAGACGGGCA AGGAGGGGAC CGAGAATCAA 
GGCGTTGAGC CTCAAGATGA AGTAGATGGA GGAGATACAC AAAAGAAGCA ACTCATAAAT 
CCTCATGTGG AACTTCAATT TTCAGATGCT AATGCCAAGT TTTACTGTCG GCTCTACTAT 
GCGGGAGAGT TTCATAAGAT GCGTGAAGTG ATTCTGGACA GCAGTGAAGA AGATTTCATT 
CGTTCCCTCT CCCACTCATC ACCCTGGCAG GCCCGGGGAG GCAAATCAGG AGCTGCCTTC 
TATGCAACTG AGGATGATAG ATTTATTTTG AAGCAAATGC CTCGTCTGGA AGTCCAGTCC 
TTCCTCGACT TTGCACCACA TTACTTCAAT TATATTACAA ATGCTGTTCA ACAAAAGAGG 
CCCACGGCGT TGGCCAAAAT TCTTGGAGTT TACAGAATTG GTTATAAGAA CTCTCAGAAC 
AACACTGAGA AGAAGTTAGA TCTCCTTGTC ATGGAAAATC TTTTCTACGG GAGAAAGATG 
GCACAGGTTT TTGATTTGAA GGGCTCTCTT AGGAATCGGA ATGTAAAAAC TGACACTGGA 
AAAGAGAGTT GTGATGTGGT CCTGCTAGAT GAAAATCTCC TAAAGATGGT TCGAGACAAC 
CCTCTATATA TTCGTTCTCA TTCCAAAGCT GTGCTGAGAA CCTCGATCCA TAGTGACTCC 
CATTTCCTTT CTAGCCACCT CATTATAGAT TATTCTTTGC TGGTTGGGCG AGATGATACT 
AGCAATGAGC TAGTAGTTGG AATTATAGAT TATATTCGAA CATTTACATG GGACAAAAAG 
CTTGAGATGG TTGTGAAATC AACAGGAATT TTAGGTGGAC AAGGTAAAAT GCCAACAGTG 
GTGTCTCCGG AGTTGTACAG GACTAGGTTT TGTGAGGCAA TGGACAAGTA TTTCCTAATG 
GTACCAGACC ACTGGACAGG CTTGGGTCTG AATTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PIKFYVE ( XM_017003569.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016859058.1 Homo sapiens phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X10, mRNA.

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CloneID	OHu89144
Clone ID Related Accession (Same CDS sequence)	XM_017003569.1
Accession Version	XM_017003569.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6111bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	1-phosphatidylinositol 3-phosphate 5-kinase isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG ATGATAAGAC GTCCCCAACA CTGGACTCTG CTAATGATTT GCCTCGATCT 
CCTACTAGTC CTTCTCATCT CACACACTTT AAACCTTTGA CTCCTGATCA AGATGAGCCC 
CCTTTTAAAT CAGCTTATAG TTCTTTTGTA AATCTCTTTC GTTTTAACAA AGAGAGAGCA 
GAAGGAGGCC AGGGAGAACA GCAGCCTTTG AGTGGAAGTT GGACCAGCCC TCAGCTCCCT 
TCGAGGACAC AGTCTGTTAG GTCACCCACA CCTTATAAAA AGCAGCTTAA TGAGGAACTC 
CAGCGGCGCT CTTCAGCATT AGACACAAGA AGGAAAGCAG AACCTACCTT TGGAGGTCAT 
GACCCTCGTA CAGCTGTTCA GCTTCGAAGC CTCAGCACAG TATTAAAACG CCTCAAGGAA 
ATCATGGAGG GGAAAAGCCA GGATAGTGAC CTGAAACAAT ACTGGATGCC AGATAGCCAA 
TGTAAAGAGT GCTATGACTG TAGTGAGAAA TTTACAACCT TTAGGCGCAG ACACCATTGC 
CGACTATGTG GGCAGATTTT CTGCAGTCGT TGCTGTAATC AAGAAATCCC TGGAAAATTT 
ATGGGCTATA CAGGAGACCT CCGAGCTTGC ACATATTGTA GAAAAATAGC CTTAAGTTAT 
GCTCATTCCA CAGACAGTAA TTCTATTGGG GAAGACTTGA ATGCTCTTTC AGATTCTGCT 
TGCTCTGTGT CTGTGCTTGA TCCAAGTGAA CCCCGAACAC CTGTTGGGAG TAGGAAAGCC 
AGCCGTAACA TATTTTTAGA GGATGATTTG GCCTGGCAAA GTTTGATTCA TCCAGATTCC 
TCAAATACTC CTCTTTCAAC AAGACTTGTA TCTGTGCAAG AGGATGCTGG GAAATCTCCT 
GCTCGAAATA GATCAGCCAG CATTACTAAC CTGTCACTGG ATAGATCTGG TTCTCCTATG 
GTACCTTCAT ATGAGACATC TGTCAGTCCC CAGGCTAACC GAACATATGT TAGGACAGAG 
ACCACTGAGG ATGAACGCAA AATTCTTCTG GACAGTGTGC AGTTAAAAGA CCTGTGGAAA 
AAAATCTGCC ATCACAGCAG TGGAATGGAG TTTCAGGATC ACCGCTACTG GTTGAGAACG 
CATCCCAACT GCATTGTAGG AAAGGAATTA GTCAACTGGC TAATCCGAAA TGGGCATATT 
GCCACAAGGG CACAAGCTAT AGCAATTGGA CAAGCAATGG TTGATGGACG TTGGCTGGAT 
TGTGTTAGTC ATCACGACCA GCTTTTCAGA GATGAGTATG CGCTGTATAG ACCACTGCAG 
AGTACAGAAT TTTCTGAGAC GCCTTCTCCC GACAGTGACT CAGTGAACTC CGTGGAAGGA 
CACTCTGAGC CATCCTGGTT TAAAGACATA AAGTTTGATG ACAGTGACAC AGAACAGATA 
GCTGAAGAAG AGTATTTGAT TTCTGACACT GGAGGACAAC AGCTCTCAAT AAGTGACGCT 
TTCATCAAAG AATCCTTATT TAATCGCCGA GTAGAGGAAA AATCCAAAGA GCTGCCTTTC 
ACACCTTTGG GCTGGCATCA TAACAACCTG GAGCTCCTGA GGGAGGAGAA TGGGGAGAAA 
CAAGCCATGG AGAGGTTGCT TTCAGCTAAT CATAACCACA TGATGGCACT ACTCCAGCAG 
TTGCTCCATA GTGACTCACT GTCATCATCT TGGAGGGACA TCATCGTGTC ATTGGTCTGC 
CAGGTTGTTC AGACAGTCCG ACCTGATGTC AAGAACCAGG ATGATGACAT GGATATCCGT 
CAGTTTGTCC ACATCAAAAA AATCCCAGGT GGAAAGAAGT TTGATTCTGT GGTTGTCAAT 
GGCTTTGTTT GTACCAAGAA CATTGCACAT AAAAAGATGA GTTCTTGTAT TAAAAACCCC 
AAAATTCTTC TGTTGAAGTG TTCCATTGAG TATCTCTACA GAGAAGAAAC TAAGTTTACT 
TGCATTGATC CTATTGTGCT TCAGGAAAGG GAATTCTTGA AGAATTATGT CCAGCGAATA 
GTTGATGTTC GACCCACCTT GGTTCTTGTT GAGAAAACAG TGTCTCGGAT TGCCCAGGAC 
ATGTTATTGG AACATGGCAT TACTTTGGTC ATTAATGTAA AGTCACAAGT TTTGGAACGA 
ATCAGTCGAA TGACCCAAGG TGATTTAGTG ATGTCAATGG ACCAGCTGCT TACGAAACCA 
CACCTGGGCA CTTGTCACAA ATTTTATATG CAGATATTTC AGTTGCCTAA TGAACAAACC 
AAGACACTGA TGTTTTTTGA AGGTTGTCCA CAGCACCTAG GCTGTACAAT CAAGCTAAGA 
GGAGGCTCTG ATTATGAGCT GGCTCGAGTT AAGGAGATCC TAATATTTAT GATCTGTGTT 
GCTTATCATT CTCAACTAGA AATATCCTTT CTCATGGATG AATTTGCTAT GCCTCCCACA 
TTAATGCAAA ACCCTTCATT CCATTCCCTG ATTGAGGGAC GAGGGCATGA GGGGGCTGTC 
CAAGAGCAGT ACGGTGGAGG TTCCATCCCC TGGGATCCTG ACATCCCTCC TGAGTCTCTG 
CCCTGTGATG ATAGCAGTTT GCTGGAATTG AGGATTGTGT TTGAGAAGGG TGAGCAGGAA 
AATAAAAATC TTCCGCAGGC TGTTGCCTCT GTGAAGCATC AAGAACATAG CACAACAGCT 
TGCCCGGCGG GTCTCCCTTG TGCTTTCTTT GCACCTGTAC CGGAATCATT GTTGCCACTC 
CCTGTGGATG ACCAACAAGA TGCTTTAGGC AGCGAGCAGC CAGAGACTTT GCAGCAAACA 
GTTGTGCTGC AGGATCCCAA AAGCCAGATA AGAGCCTTTA GAGACCCTCT ACAGGATGAC 
ACTGGATTAT ATGTTACTGA GGAAGTCACC TCCTCTGAAG ATAAACGAAA GACTTATTCT 
TTGGCCTTTA AGCAGGAATT AAAAGATGTG ATCCTCTGTA TCTCCCCAGT AATCACATTC 
CGAGAACCCT TTCTTTTAAC TGAAAAGGGG ATGAGATGCT CTACCCGAGA TTATTTTGCA 
GAGCAGGTTT ACTGGTCTCC TCTCCTCAAT AAAGAATTCA AAGAAATGGA GAACAGGAGG 
AAGAAACAGC TGCTCAGGGA TCTCTCTGGA CTTCAGGGCA TGAATGGAAG TATTCAGGCC 
AAGTCTATTC AAGTCTTACC CTCACATGAG CTAGTGAGCA CTAGAATTGC TGAGCATCTG 
GGCGATAGCC AGAGCTTGGG TAGAATGCTG GCCGATTATC GAGCCAGAGG AGGAAGAATT 
CAGCCCAAAA ATTCAGACCC TTTTGCTCAT TCAAAGGATG CATCAAGTAC TTCAAGTGGC 
CAATCAGGAA GCAAAAATGA GGGTGATGAA GAGAGAGGGC TTATTCTGAG TGATGCTGTG 
TGGTCAACAA AGGTGGACTG TCTGAATCCC ATTAATCACC AGAGACTTTG TGTGCTCTTC 
AGCAGCTCTT CTGCCCAGTC CAGCAATGCT CCTAGTGCCT GTGTCAGTCC TTGGATTGTA 
ACAATGGAAT TTTATGGAAA GAATGATCTT ACATTAGGAA TATTTTTAGA GAGATACTGT 
TTCAGGCCTT CTTATCAGTG TCCAAGCATG TTCTGTGATA CCCCCATGGT ACATCATATT 
CGGCGCTTTG TTCATGGCCA AGGCTGTGTG CAGATAATCC TGAAGGAGTT GGATTCTCCA 
GTACCTGGAT ATCAGCATAC AATTCTTACA TATTCCTGGT GTAGAATCTG CAAACAGGTA 
ACACCAGTTG TTGCTCTTTC CAATGAGTCC TGGTCTATGT CATTTGCAAA ATACCTTGAA 
CTTAGGTTTT ATGGGCACCA GTATACTCGC AGAGCCAACG CTGAGCCCTG TGGTCACTCC 
ATCCATCATG ATTATCACCA GTATTTCTCC TATAACCAGA TGGTGGCGTC TTTCAGTTAT 
TCTCCCATTC GGCTTCTTGA AGTATGTGTT CCACTCCCCA AAATATTCAT TAAGCGTCAG 
GCCCCATTAA AAGTGTCCCT TCTTCAGGAT CTGAAGGACT TCTTTCAAAA AGTTTCACAG 
GTATATGTTG CCATTGATGA AAGACTTGCA TCTTTGAAAA CTGATACATT TAGTAAAACA 
AGAGAGGAAA AAATGGAAGA TATTTTTGCA CAGAAAGAGA TGGAAGAAGG TGAGTTCAAG 
AACTGGATTG AGAAGATGCA AGCAAGGCTC ATGTCTTCCT CTGTAGATAC CCCTCAGCAA 
CTGCAGTCGG TCTTTGAGTC ACTCATTGCC AAGAAACAAA GTCTCTGTGA AGTGCTGCAA 
GCTTGGAATA ACAGGTTGCA GGACCTTTTC CAACAGGAAA AGGGTAGAAA GAGACCTTCA 
GTTCCTCCAA GTCCTGGAAG ACTGAGACAA GGGGAAGAAA GCAAGATAAG TGCGATGGAT 
GCATCTCCAC GGAATATTTC TCCAGGACTT CAGAATGGAG AAAAAGAGGA TCGCTTCTTA 
ACAACTTTGT CCAGCCAGAG CTCCACCAGT TCTACTCATC TCCAATTGCC TACGCCACCT 
GAAGTCATGT CTGAACAGTC AGTGGGAGGG CCCCCTGAGC TAGATACAGC CAGCAGTTCC 
GAAGATGTGT TTGATGGGCA TTTGCTGGGA TCCACAGACA GCCAAGTGAA GGAAAAGTCA 
ACCATGAAAG CCATCTTTGC AAATTTGCTT CCAGGAAATA GCTATAATCC TATTCCATTT 
CCTTTTGATC CAGATAAACA CTACTTAATG TATGAACATG AACGAGTGCC CATTGCAGTC 
TGCGAGAAGG AACCCAGCTC CATCATTGCT TTTGCTCTCA GTTGTAAAGA ATACCGAAAT 
GCCTTAGAGG AATTGTCTAA AGCGACTCAG TGGAACAGTG CCGAAGAAGG GCTTCCAACA 
AATAGTACTT CAGATAGCAG ACCAAAGAGT AGCAGCCCTA TCAGATTACC TGAAATGAGT 
GGAGGACAGA CAAATCGTAC AACAGAAACA GAACCACAAC CAACCAAAAA GGCTTCTGGA 
ATGTTGTCCT TCTTCAGAGG GACAGCAGGG AAAAGCCCCG ATCTCTCTTC CCAGAAGAGA 
GAGACCTTAC GTGGAGCAGA TAGTGCTTAC TACCAGGTTG GGCAGACGGG CAAGGAGGGG 
ACCGAGAATC AAGGCGTTGA GCCTCAAGAT GAAGTAGATG GAGGAGATAC ACAAAAGAAG 
CAACTCATAA ATCCTCATGT GGAACTTCAA TTTTCAGATG CTAATGCCAA GTTTTACTGT 
CGGCTCTACT ATGCGGGAGA GTTTCATAAG ATGCGTGAAG TGATTCTGGA CAGCAGTGAA 
GAAGATTTCA TTCGTTCCCT CTCCCACTCA TCACCCTGGC AGGCCCGGGG AGGCAAATCA 
GGAGCTGCCT TCTATGCAAC TGAGGATGAT AGATTTATTT TGAAGCAAAT GCCTCGTCTG 
GAAGTCCAGT CCTTCCTCGA CTTTGCACCA CATTACTTCA ATTATATTAC AAATGCTGTT 
CAACAAAAGA GGCCCACGGC GTTGGCCAAA ATTCTTGGAG TTTACAGAAT TGGTTATAAG 
AACTCTCAGA ACAACACTGA GAAGAAGTTA GATCTCCTTG TCATGGAAAA TCTTTTCTAC 
GGGAGAAAGA TGGCACAGGT TTTTGATTTG AAGGGCTCTC TTAGGAATCG GAATGTAAAA 
ACTGACACTG GAAAAGAGAG TTGTGATGTG GTCCTGCTAG ATGAAAATCT CCTAAAGATG 
GTTCGAGACA ACCCTCTATA TATTCGTTCT CATTCCAAAG CTGTGCTGAG AACCTCGATC 
CATAGTGACT CCCATTTCCT TTCTAGCCAC CTCATTATAG ATTATTCTTT GCTGGTTGGG 
CGAGATGATA CTAGCAATGA GCTAGTAGTT GGAATTATAG ATTATATTCG AACATTTACA 
TGGGACAAAA AGCTTGAGAT GGTTGTGAAA TCAACAGGAA TTTTAGGTGG ACAAGGTAAA 
ATGCCAACAG TGGTGTCTCC GGAGTTGTAC AGGACTAGGT TTTGTGAGGC AATGGACAAG 
TATTTCCTAA TGGTACCAGA CCACTGGACA GGCTTGGGTC TGAATTGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016859058.1
CDS	63..6173
Translation	MATDDKTSPTLDSANDLPRSPTSPSHLTHFKPLTPDQDEPPFKSAYSSFVNLFRFNKERAEGGQGEQQPLSGSWTSPQLPSRTQSVRSPTPYKKQLNEELQRRSSALDTRRKAEPTFGGHDPRTAVQLRSLSTVLKRLKEIMEGKSQDSDLKQYWMPDSQCKECYDCSEKFTTFRRRHHCRLCGQIFCSRCCNQEIPGKFMGYTGDLRACTYCRKIALSYAHSTDSNSIGEDLNALSDSACSVSVLDPSEPRTPVGSRKASRNIFLEDDLAWQSLIHPDSSNTPLSTRLVSVQEDAGKSPARNRSASITNLSLDRSGSPMVPSYETSVSPQANRTYVRTETTEDERKILLDSVQLKDLWKKICHHSSGMEFQDHRYWLRTHPNCIVGKELVNWLIRNGHIATRAQAIAIGQAMVDGRWLDCVSHHDQLFRDEYALYRPLQSTEFSETPSPDSDSVNSVEGHSEPSWFKDIKFDDSDTEQIAEEEYLISDTGGQQLSISDAFIKESLFNRRVEEKSKELPFTPLGWHHNNLELLREENGEKQAMERLLSANHNHMMALLQQLLHSDSLSSSWRDIIVSLVCQVVQTVRPDVKNQDDDMDIRQFVHIKKIPGGKKFDSVVVNGFVCTKNIAHKKMSSCIKNPKILLLKCSIEYLYREETKFTCIDPIVLQEREFLKNYVQRIVDVRPTLVLVEKTVSRIAQDMLLEHGITLVINVKSQVLERISRMTQGDLVMSMDQLLTKPHLGTCHKFYMQIFQLPNEQTKTLMFFEGCPQHLGCTIKLRGGSDYELARVKEILIFMICVAYHSQLEISFLMDEFAMPPTLMQNPSFHSLIEGRGHEGAVQEQYGGGSIPWDPDIPPESLPCDDSSLLELRIVFEKGEQENKNLPQAVASVKHQEHSTTACPAGLPCAFFAPVPESLLPLPVDDQQDALGSEQPETLQQTVVLQDPKSQIRAFRDPLQDDTGLYVTEEVTSSEDKRKTYSLAFKQELKDVILCISPVITFREPFLLTEKGMRCSTRDYFAEQVYWSPLLNKEFKEMENRRKKQLLRDLSGLQGMNGSIQAKSIQVLPSHELVSTRIAEHLGDSQSLGRMLADYRARGGRIQPKNSDPFAHSKDASSTSSGQSGSKNEGDEERGLILSDAVWSTKVDCLNPINHQRLCVLFSSSSAQSSNAPSACVSPWIVTMEFYGKNDLTLGIFLERYCFRPSYQCPSMFCDTPMVHHIRRFVHGQGCVQIILKELDSPVPGYQHTILTYSWCRICKQVTPVVALSNESWSMSFAKYLELRFYGHQYTRRANAEPCGHSIHHDYHQYFSYNQMVASFSYSPIRLLEVCVPLPKIFIKRQAPLKVSLLQDLKDFFQKVSQVYVAIDERLASLKTDTFSKTREEKMEDIFAQKEMEEGEFKNWIEKMQARLMSSSVDTPQQLQSVFESLIAKKQSLCEVLQAWNNRLQDLFQQEKGRKRPSVPPSPGRLRQGEESKISAMDASPRNISPGLQNGEKEDRFLTTLSSQSSTSSTHLQLPTPPEVMSEQSVGGPPELDTASSSEDVFDGHLLGSTDSQVKEKSTMKAIFANLLPGNSYNPIPFPFDPDKHYLMYEHERVPIAVCEKEPSSIIAFALSCKEYRNALEELSKATQWNSAEEGLPTNSTSDSRPKSSSPIRLPEMSGGQTNRTTETEPQPTKKASGMLSFFRGTAGKSPDLSSQKRETLRGADSAYYQVGQTGKEGTENQGVEPQDEVDGGDTQKKQLINPHVELQFSDANAKFYCRLYYAGEFHKMREVILDSSEEDFIRSLSHSSPWQARGGKSGAAFYATEDDRFILKQMPRLEVQSFLDFAPHYFNYITNAVQQKRPTALAKILGVYRIGYKNSQNNTEKKLDLLVMENLFYGRKMAQVFDLKGSLRNRNVKTDTGKESCDVVLLDENLLKMVRDNPLYIRSHSKAVLRTSIHSDSHFLSSHLIIDYSLLVGRDDTSNELVVGIIDYIRTFTWDKKLEMVVKSTGILGGQGKMPTVVSPELYRTRFCEAMDKYFLMVPDHWTGLGLNC

Target ORF information:

RefSeq Version	XM_017003569.1
Organism	Homo sapiens(human)
Definition	Homo sapiens phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017003569.1

ORF Insert Sequence:

ATGGCCACAG ATGATAAGAC GTCCCCAACA CTGGACTCTG CTAATGATTT GCCTCGATCT 
CCTACTAGTC CTTCTCATCT CACACACTTT AAACCTTTGA CTCCTGATCA AGATGAGCCC 
CCTTTTAAAT CAGCTTATAG TTCTTTTGTA AATCTCTTTC GTTTTAACAA AGAGAGAGCA 
GAAGGAGGCC AGGGAGAACA GCAGCCTTTG AGTGGAAGTT GGACCAGCCC TCAGCTCCCT 
TCGAGGACAC AGTCTGTTAG GTCACCCACA CCTTATAAAA AGCAGCTTAA TGAGGAACTC 
CAGCGGCGCT CTTCAGCATT AGACACAAGA AGGAAAGCAG AACCTACCTT TGGAGGTCAT 
GACCCTCGTA CAGCTGTTCA GCTTCGAAGC CTCAGCACAG TATTAAAACG CCTCAAGGAA 
ATCATGGAGG GGAAAAGCCA GGATAGTGAC CTGAAACAAT ACTGGATGCC AGATAGCCAA 
TGTAAAGAGT GCTATGACTG TAGTGAGAAA TTTACAACCT TTAGGCGCAG ACACCATTGC 
CGACTATGTG GGCAGATTTT CTGCAGTCGT TGCTGTAATC AAGAAATCCC TGGAAAATTT 
ATGGGCTATA CAGGAGACCT CCGAGCTTGC ACATATTGTA GAAAAATAGC CTTAAGTTAT 
GCTCATTCCA CAGACAGTAA TTCTATTGGG GAAGACTTGA ATGCTCTTTC AGATTCTGCT 
TGCTCTGTGT CTGTGCTTGA TCCAAGTGAA CCCCGAACAC CTGTTGGGAG TAGGAAAGCC 
AGCCGTAACA TATTTTTAGA GGATGATTTG GCCTGGCAAA GTTTGATTCA TCCAGATTCC 
TCAAATACTC CTCTTTCAAC AAGACTTGTA TCTGTGCAAG AGGATGCTGG GAAATCTCCT 
GCTCGAAATA GATCAGCCAG CATTACTAAC CTGTCACTGG ATAGATCTGG TTCTCCTATG 
GTACCTTCAT ATGAGACATC TGTCAGTCCC CAGGCTAACC GAACATATGT TAGGACAGAG 
ACCACTGAGG ATGAACGCAA AATTCTTCTG GACAGTGTGC AGTTAAAAGA CCTGTGGAAA 
AAAATCTGCC ATCACAGCAG TGGAATGGAG TTTCAGGATC ACCGCTACTG GTTGAGAACG 
CATCCCAACT GCATTGTAGG AAAGGAATTA GTCAACTGGC TAATCCGAAA TGGGCATATT 
GCCACAAGGG CACAAGCTAT AGCAATTGGA CAAGCAATGG TTGATGGACG TTGGCTGGAT 
TGTGTTAGTC ATCACGACCA GCTTTTCAGA GATGAGTATG CGCTGTATAG ACCACTGCAG 
AGTACAGAAT TTTCTGAGAC GCCTTCTCCC GACAGTGACT CAGTGAACTC CGTGGAAGGA 
CACTCTGAGC CATCCTGGTT TAAAGACATA AAGTTTGATG ACAGTGACAC AGAACAGATA 
GCTGAAGAAG AGTATTTGAT TTCTGACACT GGAGGACAAC AGCTCTCAAT AAGTGACGCT 
TTCATCAAAG AATCCTTATT TAATCGCCGA GTAGAGGAAA AATCCAAAGA GCTGCCTTTC 
ACACCTTTGG GCTGGCATCA TAACAACCTG GAGCTCCTGA GGGAGGAGAA TGGGGAGAAA 
CAAGCCATGG AGAGGTTGCT TTCAGCTAAT CATAACCACA TGATGGCACT ACTCCAGCAG 
TTGCTCCATA GTGACTCACT GTCATCATCT TGGAGGGACA TCATCGTGTC ATTGGTCTGC 
CAGGTTGTTC AGACAGTCCG ACCTGATGTC AAGAACCAGG ATGATGACAT GGATATCCGT 
CAGTTTGTCC ACATCAAAAA AATCCCAGGT GGAAAGAAGT TTGATTCTGT GGTTGTCAAT 
GGCTTTGTTT GTACCAAGAA CATTGCACAT AAAAAGATGA GTTCTTGTAT TAAAAACCCC 
AAAATTCTTC TGTTGAAGTG TTCCATTGAG TATCTCTACA GAGAAGAAAC TAAGTTTACT 
TGCATTGATC CTATTGTGCT TCAGGAAAGG GAATTCTTGA AGAATTATGT CCAGCGAATA 
GTTGATGTTC GACCCACCTT GGTTCTTGTT GAGAAAACAG TGTCTCGGAT TGCCCAGGAC 
ATGTTATTGG AACATGGCAT TACTTTGGTC ATTAATGTAA AGTCACAAGT TTTGGAACGA 
ATCAGTCGAA TGACCCAAGG TGATTTAGTG ATGTCAATGG ACCAGCTGCT TACGAAACCA 
CACCTGGGCA CTTGTCACAA ATTTTATATG CAGATATTTC AGTTGCCTAA TGAACAAACC 
AAGACACTGA TGTTTTTTGA AGGTTGTCCA CAGCACCTAG GCTGTACAAT CAAGCTAAGA 
GGAGGCTCTG ATTATGAGCT GGCTCGAGTT AAGGAGATCC TAATATTTAT GATCTGTGTT 
GCTTATCATT CTCAACTAGA AATATCCTTT CTCATGGATG AATTTGCTAT GCCTCCCACA 
TTAATGCAAA ACCCTTCATT CCATTCCCTG ATTGAGGGAC GAGGGCATGA GGGGGCTGTC 
CAAGAGCAGT ACGGTGGAGG TTCCATCCCC TGGGATCCTG ACATCCCTCC TGAGTCTCTG 
CCCTGTGATG ATAGCAGTTT GCTGGAATTG AGGATTGTGT TTGAGAAGGG TGAGCAGGAA 
AATAAAAATC TTCCGCAGGC TGTTGCCTCT GTGAAGCATC AAGAACATAG CACAACAGCT 
TGCCCGGCGG GTCTCCCTTG TGCTTTCTTT GCACCTGTAC CGGAATCATT GTTGCCACTC 
CCTGTGGATG ACCAACAAGA TGCTTTAGGC AGCGAGCAGC CAGAGACTTT GCAGCAAACA 
GTTGTGCTGC AGGATCCCAA AAGCCAGATA AGAGCCTTTA GAGACCCTCT ACAGGATGAC 
ACTGGATTAT ATGTTACTGA GGAAGTCACC TCCTCTGAAG ATAAACGAAA GACTTATTCT 
TTGGCCTTTA AGCAGGAATT AAAAGATGTG ATCCTCTGTA TCTCCCCAGT AATCACATTC 
CGAGAACCCT TTCTTTTAAC TGAAAAGGGG ATGAGATGCT CTACCCGAGA TTATTTTGCA 
GAGCAGGTTT ACTGGTCTCC TCTCCTCAAT AAAGAATTCA AAGAAATGGA GAACAGGAGG 
AAGAAACAGC TGCTCAGGGA TCTCTCTGGA CTTCAGGGCA TGAATGGAAG TATTCAGGCC 
AAGTCTATTC AAGTCTTACC CTCACATGAG CTAGTGAGCA CTAGAATTGC TGAGCATCTG 
GGCGATAGCC AGAGCTTGGG TAGAATGCTG GCCGATTATC GAGCCAGAGG AGGAAGAATT 
CAGCCCAAAA ATTCAGACCC TTTTGCTCAT TCAAAGGATG CATCAAGTAC TTCAAGTGGC 
CAATCAGGAA GCAAAAATGA GGGTGATGAA GAGAGAGGGC TTATTCTGAG TGATGCTGTG 
TGGTCAACAA AGGTGGACTG TCTGAATCCC ATTAATCACC AGAGACTTTG TGTGCTCTTC 
AGCAGCTCTT CTGCCCAGTC CAGCAATGCT CCTAGTGCCT GTGTCAGTCC TTGGATTGTA 
ACAATGGAAT TTTATGGAAA GAATGATCTT ACATTAGGAA TATTTTTAGA GAGATACTGT 
TTCAGGCCTT CTTATCAGTG TCCAAGCATG TTCTGTGATA CCCCCATGGT ACATCATATT 
CGGCGCTTTG TTCATGGCCA AGGCTGTGTG CAGATAATCC TGAAGGAGTT GGATTCTCCA 
GTACCTGGAT ATCAGCATAC AATTCTTACA TATTCCTGGT GTAGAATCTG CAAACAGGTA 
ACACCAGTTG TTGCTCTTTC CAATGAGTCC TGGTCTATGT CATTTGCAAA ATACCTTGAA 
CTTAGGTTTT ATGGGCACCA GTATACTCGC AGAGCCAACG CTGAGCCCTG TGGTCACTCC 
ATCCATCATG ATTATCACCA GTATTTCTCC TATAACCAGA TGGTGGCGTC TTTCAGTTAT 
TCTCCCATTC GGCTTCTTGA AGTATGTGTT CCACTCCCCA AAATATTCAT TAAGCGTCAG 
GCCCCATTAA AAGTGTCCCT TCTTCAGGAT CTGAAGGACT TCTTTCAAAA AGTTTCACAG 
GTATATGTTG CCATTGATGA AAGACTTGCA TCTTTGAAAA CTGATACATT TAGTAAAACA 
AGAGAGGAAA AAATGGAAGA TATTTTTGCA CAGAAAGAGA TGGAAGAAGG TGAGTTCAAG 
AACTGGATTG AGAAGATGCA AGCAAGGCTC ATGTCTTCCT CTGTAGATAC CCCTCAGCAA 
CTGCAGTCGG TCTTTGAGTC ACTCATTGCC AAGAAACAAA GTCTCTGTGA AGTGCTGCAA 
GCTTGGAATA ACAGGTTGCA GGACCTTTTC CAACAGGAAA AGGGTAGAAA GAGACCTTCA 
GTTCCTCCAA GTCCTGGAAG ACTGAGACAA GGGGAAGAAA GCAAGATAAG TGCGATGGAT 
GCATCTCCAC GGAATATTTC TCCAGGACTT CAGAATGGAG AAAAAGAGGA TCGCTTCTTA 
ACAACTTTGT CCAGCCAGAG CTCCACCAGT TCTACTCATC TCCAATTGCC TACGCCACCT 
GAAGTCATGT CTGAACAGTC AGTGGGAGGG CCCCCTGAGC TAGATACAGC CAGCAGTTCC 
GAAGATGTGT TTGATGGGCA TTTGCTGGGA TCCACAGACA GCCAAGTGAA GGAAAAGTCA 
ACCATGAAAG CCATCTTTGC AAATTTGCTT CCAGGAAATA GCTATAATCC TATTCCATTT 
CCTTTTGATC CAGATAAACA CTACTTAATG TATGAACATG AACGAGTGCC CATTGCAGTC 
TGCGAGAAGG AACCCAGCTC CATCATTGCT TTTGCTCTCA GTTGTAAAGA ATACCGAAAT 
GCCTTAGAGG AATTGTCTAA AGCGACTCAG TGGAACAGTG CCGAAGAAGG GCTTCCAACA 
AATAGTACTT CAGATAGCAG ACCAAAGAGT AGCAGCCCTA TCAGATTACC TGAAATGAGT 
GGAGGACAGA CAAATCGTAC AACAGAAACA GAACCACAAC CAACCAAAAA GGCTTCTGGA 
ATGTTGTCCT TCTTCAGAGG GACAGCAGGG AAAAGCCCCG ATCTCTCTTC CCAGAAGAGA 
GAGACCTTAC GTGGAGCAGA TAGTGCTTAC TACCAGGTTG GGCAGACGGG CAAGGAGGGG 
ACCGAGAATC AAGGCGTTGA GCCTCAAGAT GAAGTAGATG GAGGAGATAC ACAAAAGAAG 
CAACTCATAA ATCCTCATGT GGAACTTCAA TTTTCAGATG CTAATGCCAA GTTTTACTGT 
CGGCTCTACT ATGCGGGAGA GTTTCATAAG ATGCGTGAAG TGATTCTGGA CAGCAGTGAA 
GAAGATTTCA TTCGTTCCCT CTCCCACTCA TCACCCTGGC AGGCCCGGGG AGGCAAATCA 
GGAGCTGCCT TCTATGCAAC TGAGGATGAT AGATTTATTT TGAAGCAAAT GCCTCGTCTG 
GAAGTCCAGT CCTTCCTCGA CTTTGCACCA CATTACTTCA ATTATATTAC AAATGCTGTT 
CAACAAAAGA GGCCCACGGC GTTGGCCAAA ATTCTTGGAG TTTACAGAAT TGGTTATAAG 
AACTCTCAGA ACAACACTGA GAAGAAGTTA GATCTCCTTG TCATGGAAAA TCTTTTCTAC 
GGGAGAAAGA TGGCACAGGT TTTTGATTTG AAGGGCTCTC TTAGGAATCG GAATGTAAAA 
ACTGACACTG GAAAAGAGAG TTGTGATGTG GTCCTGCTAG ATGAAAATCT CCTAAAGATG 
GTTCGAGACA ACCCTCTATA TATTCGTTCT CATTCCAAAG CTGTGCTGAG AACCTCGATC 
CATAGTGACT CCCATTTCCT TTCTAGCCAC CTCATTATAG ATTATTCTTT GCTGGTTGGG 
CGAGATGATA CTAGCAATGA GCTAGTAGTT GGAATTATAG ATTATATTCG AACATTTACA 
TGGGACAAAA AGCTTGAGAT GGTTGTGAAA TCAACAGGAA TTTTAGGTGG ACAAGGTAAA 
ATGCCAACAG TGGTGTCTCC GGAGTTGTAC AGGACTAGGT TTTGTGAGGC AATGGACAAG 
TATTTCCTAA TGGTACCAGA CCACTGGACA GGCTTGGGTC TGAATTGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PIKFYVE ( XM_011510778.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011509080.1 Homo sapiens phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X1, mRNA.

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CloneID	OHu77661
Clone ID Related Accession (Same CDS sequence)	XM_011510778.2 , XM_011510778.3 , XM_011510779.2
Accession Version	XM_011510778.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6333bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	1-phosphatidylinositol 3-phosphate 5-kinase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011510778.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG ATGATAAGAC GTCCCCAACA CTGGACTCTG CTAATGATTT GCCTCGATCT 
CCTACTAGTC CTTCTCATCT CACACACTTT AAACCTTTGA CTCCTGATCA AGATGAGCCC 
CCTTTTAAAT CAGCTTATAG TTCTTTTGTA AATCTCTTTC GTTTTAACAA AGAGAGAGCA 
GAAGGAGGCC AGGGAGAACA GCAGCCTTTG AGTGGAAGTT GGACCAGCCC TCAGCTCCCT 
TCGAGGACAC AGTCTGTTAG GTCACCCACA CCTTATAAAA AGCAGCTTAA TGAGGAACTC 
CAGCGGCGCT CTTCAGCATT AGCAGACAAC AGTTTGCAAC ATCCCCAGGA GAACACAGAC 
ACAAGAAGGA AAGCAGAACC TACCTTTGGA GGTCATGACC CTCGTACAGC TGTTCAGCTT 
CGAAGCCTCA GCACAGTATT AAAACGCCTC AAGGAAATCA TGGAGGGGAA AAGCCAGGAT 
AGTGACCTGA AACAATACTG GATGCCAGAT AGCCAATGTA AAGAGTGCTA TGACTGTAGT 
GAGAAATTTA CAACCTTTAG GCGCAGACAC CATTGCCGAC TATGTGGGCA GATTTTCTGC 
AGTCGTTGCT GTAATCAAGA AATCCCTGGA AAATTTATGG GCTATACAGG AGACCTCCGA 
GCTTGCACAT ATTGTAGAAA AATAGCCTTA AGTTATGCTC ATTCCACAGA CAGTAATTCT 
ATTGGGGAAG ACTTGAATGC TCTTTCAGAT TCTGCTTGCT CTGTGTCTGT GCTTGATCCA 
AGTGAACCCC GAACACCTGT TGGGAGTAGG AAAGCCAGCC GTAACATATT TTTAGAGGAT 
GATTTGGCCT GGCAAAGTTT GATTCATCCA GATTCCTCAA ATACTCCTCT TTCAACAAGA 
CTTGTATCTG TGCAAGAGGA TGCTGGGAAA TCTCCTGCTC GAAATAGATC AGCCAGCATT 
ACTAACCTGT CACTGGATAG ATCTGGTTCT CCTATGGTAC CTTCATATGA GACATCTGTC 
AGTCCCCAGG CTAACCGAAC ATATGTTAGG ACAGAGACCA CTGAGGATGA ACGCAAAATT 
CTTCTGGACA GTGTGCAGTT AAAAGACCTG TGGAAAAAAA TCTGCCATCA CAGCAGTGGA 
ATGGAGTTTC AGGATCACCG CTACTGGTTG AGAACGCATC CCAACTGCAT TGTAGGAAAG 
GAATTAGTCA ACTGGCTAAT CCGAAATGGG CATATTGCCA CAAGGGCACA AGCTATAGCA 
ATTGGACAAG CAATGGTTGA TGGACGTTGG CTGGATTGTG TTAGTCATCA CGACCAGCTT 
TTCAGAGATG AGTATGCGCT GTATAGACCA CTGCAGAGTA CAGAATTTTC TGAGACGCCT 
TCTCCCGACA GTGACTCAGT GAACTCCGTG GAAGGACACT CTGAGCCATC CTGGTTTAAA 
GACATAAAGT TTGATGACAG TGACACAGAA CAGATAGCTG AAGAAGGTGA CGATAATTTG 
GCTAATTCTG CCAGTCCTAG CAAGCGCACA TCAGTCAGCA GTTTCCAGTC CACAGTGGAC 
AGTGACTCAG CCGCTTCTAT CAGCCTGAAC GTGGAGCTGG ACAACGTGAA CTTCCATATC 
AAGAAGCCCT CCAAGTACCC ACATGTGCCC CCTCACCCTG CTGACCAAAA AGAGTATTTG 
ATTTCTGACA CTGGAGGACA ACAGCTCTCA ATAAGTGACG CTTTCATCAA AGAATCCTTA 
TTTAATCGCC GAGTAGAGGA AAAATCCAAA GAGCTGCCTT TCACACCTTT GGGCTGGCAT 
CATAACAACC TGGAGCTCCT GAGGGAGGAG AATGGGGAGA AACAAGCCAT GGAGAGGTTG 
CTTTCAGCTA ATCATAACCA CATGATGGCA CTACTCCAGC AGTTGCTCCA TAGTGACTCA 
CTGTCATCAT CTTGGAGGGA CATCATCGTG TCATTGGTCT GCCAGGTTGT TCAGACAGTC 
CGACCTGATG TCAAGAACCA GGATGATGAC ATGGATATCC GTCAGTTTGT CCACATCAAA 
AAAATCCCAG GTGGAAAGAA GTTTGATTCT GTGGTTGTCA ATGGCTTTGT TTGTACCAAG 
AACATTGCAC ATAAAAAGAT GAGTTCTTGT ATTAAAAACC CCAAAATTCT TCTGTTGAAG 
TGTTCCATTG AGTATCTCTA CAGAGAAGAA ACTAAGTTTA CTTGCATTGA TCCTATTGTG 
CTTCAGGAAA GGGAATTCTT GAAGAATTAT GTCCAGCGAA TAGTTGATGT TCGACCCACC 
TTGGTTCTTG TTGAGAAAAC AGTGTCTCGG ATTGCCCAGG ACATGTTATT GGAACATGGC 
ATTACTTTGG TCATTAATGT AAAGTCACAA GTTTTGGAAC GAATCAGTCG AATGACCCAA 
GGTGATTTAG TGATGTCAAT GGACCAGCTG CTTACGAAAC CACACCTGGG CACTTGTCAC 
AAATTTTATA TGCAGATATT TCAGTTGCCT AATGAACAAA CCAAGACACT GATGTTTTTT 
GAAGGTTGTC CACAGCACCT AGGCTGTACA ATCAAGCTAA GAGGAGGCTC TGATTATGAG 
CTGGCTCGAG TTAAGGAGAT CCTAATATTT ATGATCTGTG TTGCTTATCA TTCTCAACTA 
GAAATATCCT TTCTCATGGA TGAATTTGCT ATGCCTCCCA CATTAATGCA AAACCCTTCA 
TTCCATTCCC TGATTGAGGG ACGAGGGCAT GAGGGGGCTG TCCAAGAGCA GTACGGTGGA 
GGTTCCATCC CCTGGGATCC TGACATCCCT CCTGAGTCTC TGCCCTGTGA TGATAGCAGT 
TTGCTGGAAT TGAGGATTGT GTTTGAGAAG GGTGAGCAGG AAAATAAAAA TCTTCCGCAG 
GCTGTTGCCT CTGTGAAGCA TCAAGAACAT AGCACAACAG CTTGCCCGGC GGGTCTCCCT 
TGTGCTTTCT TTGCACCTGT ACCGGAATCA TTGTTGCCAC TCCCTGTGGA TGACCAACAA 
GATGCTTTAG GCAGCGAGCA GCCAGAGACT TTGCAGCAAA CAGTTGTGCT GCAGGATCCC 
AAAAGCCAGA TAAGAGCCTT TAGAGACCCT CTACAGGATG ACACTGGATT ATATGTTACT 
GAGGAAGTCA CCTCCTCTGA AGATAAACGA AAGACTTATT CTTTGGCCTT TAAGCAGGAA 
TTAAAAGATG TGATCCTCTG TATCTCCCCA GTAATCACAT TCCGAGAACC CTTTCTTTTA 
ACTGAAAAGG GGATGAGATG CTCTACCCGA GATTATTTTG CAGAGCAGGT TTACTGGTCT 
CCTCTCCTCA ATAAAGAATT CAAAGAAATG GAGAACAGGA GGAAGAAACA GCTGCTCAGG 
GATCTCTCTG GACTTCAGGG CATGAATGGA AGTATTCAGG CCAAGTCTAT TCAAGTCTTA 
CCCTCACATG AGCTAGTGAG CACTAGAATT GCTGAGCATC TGGGCGATAG CCAGAGCTTG 
GGTAGAATGC TGGCCGATTA TCGAGCCAGA GGAGGAAGAA TTCAGCCCAA AAATTCAGAC 
CCTTTTGCTC ATTCAAAGGA TGCATCAAGT ACTTCAAGTG GCCAATCAGG AAGCAAAAAT 
GAGGGTGATG AAGAGAGAGG GCTTATTCTG AGTGATGCTG TGTGGTCAAC AAAGGTGGAC 
TGTCTGAATC CCATTAATCA CCAGAGACTT TGTGTGCTCT TCAGCAGCTC TTCTGCCCAG 
TCCAGCAATG CTCCTAGTGC CTGTGTCAGT CCTTGGATTG TAACAATGGA ATTTTATGGA 
AAGAATGATC TTACATTAGG AATATTTTTA GAGAGATACT GTTTCAGGCC TTCTTATCAG 
TGTCCAAGCA TGTTCTGTGA TACCCCCATG GTACATCATA TTCGGCGCTT TGTTCATGGC 
CAAGGCTGTG TGCAGATAAT CCTGAAGGAG TTGGATTCTC CAGTACCTGG ATATCAGCAT 
ACAATTCTTA CATATTCCTG GTGTAGAATC TGCAAACAGG TAACACCAGT TGTTGCTCTT 
TCCAATGAGT CCTGGTCTAT GTCATTTGCA AAATACCTTG AACTTAGGTT TTATGGGCAC 
CAGTATACTC GCAGAGCCAA CGCTGAGCCC TGTGGTCACT CCATCCATCA TGATTATCAC 
CAGTATTTCT CCTATAACCA GATGGTGGCG TCTTTCAGTT ATTCTCCCAT TCGGCTTCTT 
GAAGTATGTG TTCCACTCCC CAAAATATTC ATTAAGCGTC AGGCCCCATT AAAAGTGTCC 
CTTCTTCAGG ATCTGAAGGA CTTCTTTCAA AAAGTTTCAC AGGTATATGT TGCCATTGAT 
GAAAGACTTG CATCTTTGAA AACTGATACA TTTAGTAAAA CAAGAGAGGA AAAAATGGAA 
GATATTTTTG CACAGAAAGA GATGGAAGAA GGTGAGTTCA AGAACTGGAT TGAGAAGATG 
CAAGCAAGGC TCATGTCTTC CTCTGTAGAT ACCCCTCAGC AACTGCAGTC GGTCTTTGAG 
TCACTCATTG CCAAGAAACA AAGTCTCTGT GAAGTGCTGC AAGCTTGGAA TAACAGGTTG 
CAGGACCTTT TCCAACAGGA AAAGGGTAGA AAGAGACCTT CAGTTCCTCC AAGTCCTGGA 
AGACTGAGAC AAGGGGAAGA AAGCAAGATA AGTGCGATGG ATGCATCTCC ACGGAATATT 
TCTCCAGGAC TTCAGAATGG AGAAAAAGAG GATCGCTTCT TAACAACTTT GTCCAGCCAG 
AGCTCCACCA GTTCTACTCA TCTCCAATTG CCTACGCCAC CTGAAGTCAT GTCTGAACAG 
TCAGTGGGAG GGCCCCCTGA GCTAGATACA GCCAGCAGTT CCGAAGATGT GTTTGATGGG 
CATTTGCTGG GATCCACAGA CAGCCAAGTG AAGGAAAAGT CAACCATGAA AGCCATCTTT 
GCAAATTTGC TTCCAGGAAA TAGCTATAAT CCTATTCCAT TTCCTTTTGA TCCAGATAAA 
CACTACTTAA TGTATGAACA TGAACGAGTG CCCATTGCAG TCTGCGAGAA GGAACCCAGC 
TCCATCATTG CTTTTGCTCT CAGTTGTAAA GAATACCGAA ATGCCTTAGA GGAATTGTCT 
AAAGCGACTC AGTGGAACAG TGCCGAAGAA GGGCTTCCAA CAAATAGTAC TTCAGATAGC 
AGACCAAAGA GTAGCAGCCC TATCAGATTA CCTGAAATGA GTGGAGGACA GACAAATCGT 
ACAACAGAAA CAGAACCACA ACCAACCAAA AAGGCTTCTG GAATGTTGTC CTTCTTCAGA 
GGGACAGCAG GGAAAAGCCC CGATCTCTCT TCCCAGAAGA GAGAGACCTT ACGTGGAGCA 
GATAGTGCTT ACTACCAGGT TGGGCAGACG GGCAAGGAGG GGACCGAGAA TCAAGGCGTT 
GAGCCTCAAG ATGAAGTAGA TGGAGGAGAT ACACAAAAGA AGCAACTCAT AAATCCTCAT 
GTGGAACTTC AATTTTCAGA TGCTAATGCC AAGTTTTACT GTCGGCTCTA CTATGCGGGA 
GAGTTTCATA AGATGCGTGA AGTGATTCTG GACAGCAGTG AAGAAGATTT CATTCGTTCC 
CTCTCCCACT CATCACCCTG GCAGGCCCGG GGAGGCAAAT CAGGAGCTGC CTTCTATGCA 
ACTGAGGATG ATAGATTTAT TTTGAAGCAA ATGCCTCGTC TGGAAGTCCA GTCCTTCCTC 
GACTTTGCAC CACATTACTT CAATTATATT ACAAATGCTG TTCAACAAAA GAGGCCCACG 
GCGTTGGCCA AAATTCTTGG AGTTTACAGA ATTGGTTATA AGAACTCTCA GAACAACACT 
GAGAAGAAGT TAGATCTCCT TGTCATGGAA AATCTTTTCT ACGGGAGAAA GATGGCACAG 
GTTTTTGATT TGAAGGGCTC TCTTAGGAAT CGGAATGTAA AAACTGACAC TGGAAAAGAG 
AGTTGTGATG TGGTCCTGCT AGATGAAAAT CTCCTAAAGA TGGTTCGAGA CAACCCTCTA 
TATATTCGTT CTCATTCCAA AGCTGTGCTG AGAACCTCGA TCCATAGTGA CTCCCATTTC 
CTTTCTAGCC ACCTCATTAT AGATTATTCT TTGCTGGTTG GGCGAGATGA TACTAGCAAT 
GAGCTAGTAG TTGGAATTAT AGATTATATT CGAACATTTA CATGGGACAA AAAGCTTGAG 
ATGGTTGTGA AATCAACAGG AATTTTAGGT GGACAAGGTA AAATGCCAAC AGTGGTGTCT 
CCGGAGTTGT ACAGGACTAG GTTTTGTGAG GCAATGGACA AGTATTTCCT AATGGTACCA 
GACCACTGGA CAGGCTTGGG TCTGAATTGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011509080.1
CDS	173..6505
Translation	MATDDKTSPTLDSANDLPRSPTSPSHLTHFKPLTPDQDEPPFKSAYSSFVNLFRFNKERAEGGQGEQQPLSGSWTSPQLPSRTQSVRSPTPYKKQLNEELQRRSSALADNSLQHPQENTDTRRKAEPTFGGHDPRTAVQLRSLSTVLKRLKEIMEGKSQDSDLKQYWMPDSQCKECYDCSEKFTTFRRRHHCRLCGQIFCSRCCNQEIPGKFMGYTGDLRACTYCRKIALSYAHSTDSNSIGEDLNALSDSACSVSVLDPSEPRTPVGSRKASRNIFLEDDLAWQSLIHPDSSNTPLSTRLVSVQEDAGKSPARNRSASITNLSLDRSGSPMVPSYETSVSPQANRTYVRTETTEDERKILLDSVQLKDLWKKICHHSSGMEFQDHRYWLRTHPNCIVGKELVNWLIRNGHIATRAQAIAIGQAMVDGRWLDCVSHHDQLFRDEYALYRPLQSTEFSETPSPDSDSVNSVEGHSEPSWFKDIKFDDSDTEQIAEEGDDNLANSASPSKRTSVSSFQSTVDSDSAASISLNVELDNVNFHIKKPSKYPHVPPHPADQKEYLISDTGGQQLSISDAFIKESLFNRRVEEKSKELPFTPLGWHHNNLELLREENGEKQAMERLLSANHNHMMALLQQLLHSDSLSSSWRDIIVSLVCQVVQTVRPDVKNQDDDMDIRQFVHIKKIPGGKKFDSVVVNGFVCTKNIAHKKMSSCIKNPKILLLKCSIEYLYREETKFTCIDPIVLQEREFLKNYVQRIVDVRPTLVLVEKTVSRIAQDMLLEHGITLVINVKSQVLERISRMTQGDLVMSMDQLLTKPHLGTCHKFYMQIFQLPNEQTKTLMFFEGCPQHLGCTIKLRGGSDYELARVKEILIFMICVAYHSQLEISFLMDEFAMPPTLMQNPSFHSLIEGRGHEGAVQEQYGGGSIPWDPDIPPESLPCDDSSLLELRIVFEKGEQENKNLPQAVASVKHQEHSTTACPAGLPCAFFAPVPESLLPLPVDDQQDALGSEQPETLQQTVVLQDPKSQIRAFRDPLQDDTGLYVTEEVTSSEDKRKTYSLAFKQELKDVILCISPVITFREPFLLTEKGMRCSTRDYFAEQVYWSPLLNKEFKEMENRRKKQLLRDLSGLQGMNGSIQAKSIQVLPSHELVSTRIAEHLGDSQSLGRMLADYRARGGRIQPKNSDPFAHSKDASSTSSGQSGSKNEGDEERGLILSDAVWSTKVDCLNPINHQRLCVLFSSSSAQSSNAPSACVSPWIVTMEFYGKNDLTLGIFLERYCFRPSYQCPSMFCDTPMVHHIRRFVHGQGCVQIILKELDSPVPGYQHTILTYSWCRICKQVTPVVALSNESWSMSFAKYLELRFYGHQYTRRANAEPCGHSIHHDYHQYFSYNQMVASFSYSPIRLLEVCVPLPKIFIKRQAPLKVSLLQDLKDFFQKVSQVYVAIDERLASLKTDTFSKTREEKMEDIFAQKEMEEGEFKNWIEKMQARLMSSSVDTPQQLQSVFESLIAKKQSLCEVLQAWNNRLQDLFQQEKGRKRPSVPPSPGRLRQGEESKISAMDASPRNISPGLQNGEKEDRFLTTLSSQSSTSSTHLQLPTPPEVMSEQSVGGPPELDTASSSEDVFDGHLLGSTDSQVKEKSTMKAIFANLLPGNSYNPIPFPFDPDKHYLMYEHERVPIAVCEKEPSSIIAFALSCKEYRNALEELSKATQWNSAEEGLPTNSTSDSRPKSSSPIRLPEMSGGQTNRTTETEPQPTKKASGMLSFFRGTAGKSPDLSSQKRETLRGADSAYYQVGQTGKEGTENQGVEPQDEVDGGDTQKKQLINPHVELQFSDANAKFYCRLYYAGEFHKMREVILDSSEEDFIRSLSHSSPWQARGGKSGAAFYATEDDRFILKQMPRLEVQSFLDFAPHYFNYITNAVQQKRPTALAKILGVYRIGYKNSQNNTEKKLDLLVMENLFYGRKMAQVFDLKGSLRNRNVKTDTGKESCDVVLLDENLLKMVRDNPLYIRSHSKAVLRTSIHSDSHFLSSHLIIDYSLLVGRDDTSNELVVGIIDYIRTFTWDKKLEMVVKSTGILGGQGKMPTVVSPELYRTRFCEAMDKYFLMVPDHWTGLGLNC

Target ORF information:

RefSeq Version	XM_011510778.2
Organism	Homo sapiens(human)
Definition	Homo sapiens phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011510778.2

ORF Insert Sequence:

ATGGCCACAG ATGATAAGAC GTCCCCAACA CTGGACTCTG CTAATGATTT GCCTCGATCT 
CCTACTAGTC CTTCTCATCT CACACACTTT AAACCTTTGA CTCCTGATCA AGATGAGCCC 
CCTTTTAAAT CAGCTTATAG TTCTTTTGTA AATCTCTTTC GTTTTAACAA AGAGAGAGCA 
GAAGGAGGCC AGGGAGAACA GCAGCCTTTG AGTGGAAGTT GGACCAGCCC TCAGCTCCCT 
TCGAGGACAC AGTCTGTTAG GTCACCCACA CCTTATAAAA AGCAGCTTAA TGAGGAACTC 
CAGCGGCGCT CTTCAGCATT AGCAGACAAC AGTTTGCAAC ATCCCCAGGA GAACACAGAC 
ACAAGAAGGA AAGCAGAACC TACCTTTGGA GGTCATGACC CTCGTACAGC TGTTCAGCTT 
CGAAGCCTCA GCACAGTATT AAAACGCCTC AAGGAAATCA TGGAGGGGAA AAGCCAGGAT 
AGTGACCTGA AACAATACTG GATGCCAGAT AGCCAATGTA AAGAGTGCTA TGACTGTAGT 
GAGAAATTTA CAACCTTTAG GCGCAGACAC CATTGCCGAC TATGTGGGCA GATTTTCTGC 
AGTCGTTGCT GTAATCAAGA AATCCCTGGA AAATTTATGG GCTATACAGG AGACCTCCGA 
GCTTGCACAT ATTGTAGAAA AATAGCCTTA AGTTATGCTC ATTCCACAGA CAGTAATTCT 
ATTGGGGAAG ACTTGAATGC TCTTTCAGAT TCTGCTTGCT CTGTGTCTGT GCTTGATCCA 
AGTGAACCCC GAACACCTGT TGGGAGTAGG AAAGCCAGCC GTAACATATT TTTAGAGGAT 
GATTTGGCCT GGCAAAGTTT GATTCATCCA GATTCCTCAA ATACTCCTCT TTCAACAAGA 
CTTGTATCTG TGCAAGAGGA TGCTGGGAAA TCTCCTGCTC GAAATAGATC AGCCAGCATT 
ACTAACCTGT CACTGGATAG ATCTGGTTCT CCTATGGTAC CTTCATATGA GACATCTGTC 
AGTCCCCAGG CTAACCGAAC ATATGTTAGG ACAGAGACCA CTGAGGATGA ACGCAAAATT 
CTTCTGGACA GTGTGCAGTT AAAAGACCTG TGGAAAAAAA TCTGCCATCA CAGCAGTGGA 
ATGGAGTTTC AGGATCACCG CTACTGGTTG AGAACGCATC CCAACTGCAT TGTAGGAAAG 
GAATTAGTCA ACTGGCTAAT CCGAAATGGG CATATTGCCA CAAGGGCACA AGCTATAGCA 
ATTGGACAAG CAATGGTTGA TGGACGTTGG CTGGATTGTG TTAGTCATCA CGACCAGCTT 
TTCAGAGATG AGTATGCGCT GTATAGACCA CTGCAGAGTA CAGAATTTTC TGAGACGCCT 
TCTCCCGACA GTGACTCAGT GAACTCCGTG GAAGGACACT CTGAGCCATC CTGGTTTAAA 
GACATAAAGT TTGATGACAG TGACACAGAA CAGATAGCTG AAGAAGGTGA CGATAATTTG 
GCTAATTCTG CCAGTCCTAG CAAGCGCACA TCAGTCAGCA GTTTCCAGTC CACAGTGGAC 
AGTGACTCAG CCGCTTCTAT CAGCCTGAAC GTGGAGCTGG ACAACGTGAA CTTCCATATC 
AAGAAGCCCT CCAAGTACCC ACATGTGCCC CCTCACCCTG CTGACCAAAA AGAGTATTTG 
ATTTCTGACA CTGGAGGACA ACAGCTCTCA ATAAGTGACG CTTTCATCAA AGAATCCTTA 
TTTAATCGCC GAGTAGAGGA AAAATCCAAA GAGCTGCCTT TCACACCTTT GGGCTGGCAT 
CATAACAACC TGGAGCTCCT GAGGGAGGAG AATGGGGAGA AACAAGCCAT GGAGAGGTTG 
CTTTCAGCTA ATCATAACCA CATGATGGCA CTACTCCAGC AGTTGCTCCA TAGTGACTCA 
CTGTCATCAT CTTGGAGGGA CATCATCGTG TCATTGGTCT GCCAGGTTGT TCAGACAGTC 
CGACCTGATG TCAAGAACCA GGATGATGAC ATGGATATCC GTCAGTTTGT CCACATCAAA 
AAAATCCCAG GTGGAAAGAA GTTTGATTCT GTGGTTGTCA ATGGCTTTGT TTGTACCAAG 
AACATTGCAC ATAAAAAGAT GAGTTCTTGT ATTAAAAACC CCAAAATTCT TCTGTTGAAG 
TGTTCCATTG AGTATCTCTA CAGAGAAGAA ACTAAGTTTA CTTGCATTGA TCCTATTGTG 
CTTCAGGAAA GGGAATTCTT GAAGAATTAT GTCCAGCGAA TAGTTGATGT TCGACCCACC 
TTGGTTCTTG TTGAGAAAAC AGTGTCTCGG ATTGCCCAGG ACATGTTATT GGAACATGGC 
ATTACTTTGG TCATTAATGT AAAGTCACAA GTTTTGGAAC GAATCAGTCG AATGACCCAA 
GGTGATTTAG TGATGTCAAT GGACCAGCTG CTTACGAAAC CACACCTGGG CACTTGTCAC 
AAATTTTATA TGCAGATATT TCAGTTGCCT AATGAACAAA CCAAGACACT GATGTTTTTT 
GAAGGTTGTC CACAGCACCT AGGCTGTACA ATCAAGCTAA GAGGAGGCTC TGATTATGAG 
CTGGCTCGAG TTAAGGAGAT CCTAATATTT ATGATCTGTG TTGCTTATCA TTCTCAACTA 
GAAATATCCT TTCTCATGGA TGAATTTGCT ATGCCTCCCA CATTAATGCA AAACCCTTCA 
TTCCATTCCC TGATTGAGGG ACGAGGGCAT GAGGGGGCTG TCCAAGAGCA GTACGGTGGA 
GGTTCCATCC CCTGGGATCC TGACATCCCT CCTGAGTCTC TGCCCTGTGA TGATAGCAGT 
TTGCTGGAAT TGAGGATTGT GTTTGAGAAG GGTGAGCAGG AAAATAAAAA TCTTCCGCAG 
GCTGTTGCCT CTGTGAAGCA TCAAGAACAT AGCACAACAG CTTGCCCGGC GGGTCTCCCT 
TGTGCTTTCT TTGCACCTGT ACCGGAATCA TTGTTGCCAC TCCCTGTGGA TGACCAACAA 
GATGCTTTAG GCAGCGAGCA GCCAGAGACT TTGCAGCAAA CAGTTGTGCT GCAGGATCCC 
AAAAGCCAGA TAAGAGCCTT TAGAGACCCT CTACAGGATG ACACTGGATT ATATGTTACT 
GAGGAAGTCA CCTCCTCTGA AGATAAACGA AAGACTTATT CTTTGGCCTT TAAGCAGGAA 
TTAAAAGATG TGATCCTCTG TATCTCCCCA GTAATCACAT TCCGAGAACC CTTTCTTTTA 
ACTGAAAAGG GGATGAGATG CTCTACCCGA GATTATTTTG CAGAGCAGGT TTACTGGTCT 
CCTCTCCTCA ATAAAGAATT CAAAGAAATG GAGAACAGGA GGAAGAAACA GCTGCTCAGG 
GATCTCTCTG GACTTCAGGG CATGAATGGA AGTATTCAGG CCAAGTCTAT TCAAGTCTTA 
CCCTCACATG AGCTAGTGAG CACTAGAATT GCTGAGCATC TGGGCGATAG CCAGAGCTTG 
GGTAGAATGC TGGCCGATTA TCGAGCCAGA GGAGGAAGAA TTCAGCCCAA AAATTCAGAC 
CCTTTTGCTC ATTCAAAGGA TGCATCAAGT ACTTCAAGTG GCCAATCAGG AAGCAAAAAT 
GAGGGTGATG AAGAGAGAGG GCTTATTCTG AGTGATGCTG TGTGGTCAAC AAAGGTGGAC 
TGTCTGAATC CCATTAATCA CCAGAGACTT TGTGTGCTCT TCAGCAGCTC TTCTGCCCAG 
TCCAGCAATG CTCCTAGTGC CTGTGTCAGT CCTTGGATTG TAACAATGGA ATTTTATGGA 
AAGAATGATC TTACATTAGG AATATTTTTA GAGAGATACT GTTTCAGGCC TTCTTATCAG 
TGTCCAAGCA TGTTCTGTGA TACCCCCATG GTACATCATA TTCGGCGCTT TGTTCATGGC 
CAAGGCTGTG TGCAGATAAT CCTGAAGGAG TTGGATTCTC CAGTACCTGG ATATCAGCAT 
ACAATTCTTA CATATTCCTG GTGTAGAATC TGCAAACAGG TAACACCAGT TGTTGCTCTT 
TCCAATGAGT CCTGGTCTAT GTCATTTGCA AAATACCTTG AACTTAGGTT TTATGGGCAC 
CAGTATACTC GCAGAGCCAA CGCTGAGCCC TGTGGTCACT CCATCCATCA TGATTATCAC 
CAGTATTTCT CCTATAACCA GATGGTGGCG TCTTTCAGTT ATTCTCCCAT TCGGCTTCTT 
GAAGTATGTG TTCCACTCCC CAAAATATTC ATTAAGCGTC AGGCCCCATT AAAAGTGTCC 
CTTCTTCAGG ATCTGAAGGA CTTCTTTCAA AAAGTTTCAC AGGTATATGT TGCCATTGAT 
GAAAGACTTG CATCTTTGAA AACTGATACA TTTAGTAAAA CAAGAGAGGA AAAAATGGAA 
GATATTTTTG CACAGAAAGA GATGGAAGAA GGTGAGTTCA AGAACTGGAT TGAGAAGATG 
CAAGCAAGGC TCATGTCTTC CTCTGTAGAT ACCCCTCAGC AACTGCAGTC GGTCTTTGAG 
TCACTCATTG CCAAGAAACA AAGTCTCTGT GAAGTGCTGC AAGCTTGGAA TAACAGGTTG 
CAGGACCTTT TCCAACAGGA AAAGGGTAGA AAGAGACCTT CAGTTCCTCC AAGTCCTGGA 
AGACTGAGAC AAGGGGAAGA AAGCAAGATA AGTGCGATGG ATGCATCTCC ACGGAATATT 
TCTCCAGGAC TTCAGAATGG AGAAAAAGAG GATCGCTTCT TAACAACTTT GTCCAGCCAG 
AGCTCCACCA GTTCTACTCA TCTCCAATTG CCTACGCCAC CTGAAGTCAT GTCTGAACAG 
TCAGTGGGAG GGCCCCCTGA GCTAGATACA GCCAGCAGTT CCGAAGATGT GTTTGATGGG 
CATTTGCTGG GATCCACAGA CAGCCAAGTG AAGGAAAAGT CAACCATGAA AGCCATCTTT 
GCAAATTTGC TTCCAGGAAA TAGCTATAAT CCTATTCCAT TTCCTTTTGA TCCAGATAAA 
CACTACTTAA TGTATGAACA TGAACGAGTG CCCATTGCAG TCTGCGAGAA GGAACCCAGC 
TCCATCATTG CTTTTGCTCT CAGTTGTAAA GAATACCGAA ATGCCTTAGA GGAATTGTCT 
AAAGCGACTC AGTGGAACAG TGCCGAAGAA GGGCTTCCAA CAAATAGTAC TTCAGATAGC 
AGACCAAAGA GTAGCAGCCC TATCAGATTA CCTGAAATGA GTGGAGGACA GACAAATCGT 
ACAACAGAAA CAGAACCACA ACCAACCAAA AAGGCTTCTG GAATGTTGTC CTTCTTCAGA 
GGGACAGCAG GGAAAAGCCC CGATCTCTCT TCCCAGAAGA GAGAGACCTT ACGTGGAGCA 
GATAGTGCTT ACTACCAGGT TGGGCAGACG GGCAAGGAGG GGACCGAGAA TCAAGGCGTT 
GAGCCTCAAG ATGAAGTAGA TGGAGGAGAT ACACAAAAGA AGCAACTCAT AAATCCTCAT 
GTGGAACTTC AATTTTCAGA TGCTAATGCC AAGTTTTACT GTCGGCTCTA CTATGCGGGA 
GAGTTTCATA AGATGCGTGA AGTGATTCTG GACAGCAGTG AAGAAGATTT CATTCGTTCC 
CTCTCCCACT CATCACCCTG GCAGGCCCGG GGAGGCAAAT CAGGAGCTGC CTTCTATGCA 
ACTGAGGATG ATAGATTTAT TTTGAAGCAA ATGCCTCGTC TGGAAGTCCA GTCCTTCCTC 
GACTTTGCAC CACATTACTT CAATTATATT ACAAATGCTG TTCAACAAAA GAGGCCCACG 
GCGTTGGCCA AAATTCTTGG AGTTTACAGA ATTGGTTATA AGAACTCTCA GAACAACACT 
GAGAAGAAGT TAGATCTCCT TGTCATGGAA AATCTTTTCT ACGGGAGAAA GATGGCACAG 
GTTTTTGATT TGAAGGGCTC TCTTAGGAAT CGGAATGTAA AAACTGACAC TGGAAAAGAG 
AGTTGTGATG TGGTCCTGCT AGATGAAAAT CTCCTAAAGA TGGTTCGAGA CAACCCTCTA 
TATATTCGTT CTCATTCCAA AGCTGTGCTG AGAACCTCGA TCCATAGTGA CTCCCATTTC 
CTTTCTAGCC ACCTCATTAT AGATTATTCT TTGCTGGTTG GGCGAGATGA TACTAGCAAT 
GAGCTAGTAG TTGGAATTAT AGATTATATT CGAACATTTA CATGGGACAA AAAGCTTGAG 
ATGGTTGTGA AATCAACAGG AATTTTAGGT GGACAAGGTA AAATGCCAAC AGTGGTGTCT 
CCGGAGTTGT ACAGGACTAG GTTTTGTGAG GCAATGGACA AGTATTTCCT AATGGTACCA 
GACCACTGGA CAGGCTTGGG TCTGAATTGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PIKFYVE ( XM_011510778.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011509080.1 Homo sapiens phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X1, mRNA.

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CloneID	OHu77661
Clone ID Related Accession (Same CDS sequence)	XM_011510778.2 , XM_011510778.3 , XM_011510779.2
Accession Version	XM_011510778.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6333bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	1-phosphatidylinositol 3-phosphate 5-kinase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011510778.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG ATGATAAGAC GTCCCCAACA CTGGACTCTG CTAATGATTT GCCTCGATCT 
CCTACTAGTC CTTCTCATCT CACACACTTT AAACCTTTGA CTCCTGATCA AGATGAGCCC 
CCTTTTAAAT CAGCTTATAG TTCTTTTGTA AATCTCTTTC GTTTTAACAA AGAGAGAGCA 
GAAGGAGGCC AGGGAGAACA GCAGCCTTTG AGTGGAAGTT GGACCAGCCC TCAGCTCCCT 
TCGAGGACAC AGTCTGTTAG GTCACCCACA CCTTATAAAA AGCAGCTTAA TGAGGAACTC 
CAGCGGCGCT CTTCAGCATT AGCAGACAAC AGTTTGCAAC ATCCCCAGGA GAACACAGAC 
ACAAGAAGGA AAGCAGAACC TACCTTTGGA GGTCATGACC CTCGTACAGC TGTTCAGCTT 
CGAAGCCTCA GCACAGTATT AAAACGCCTC AAGGAAATCA TGGAGGGGAA AAGCCAGGAT 
AGTGACCTGA AACAATACTG GATGCCAGAT AGCCAATGTA AAGAGTGCTA TGACTGTAGT 
GAGAAATTTA CAACCTTTAG GCGCAGACAC CATTGCCGAC TATGTGGGCA GATTTTCTGC 
AGTCGTTGCT GTAATCAAGA AATCCCTGGA AAATTTATGG GCTATACAGG AGACCTCCGA 
GCTTGCACAT ATTGTAGAAA AATAGCCTTA AGTTATGCTC ATTCCACAGA CAGTAATTCT 
ATTGGGGAAG ACTTGAATGC TCTTTCAGAT TCTGCTTGCT CTGTGTCTGT GCTTGATCCA 
AGTGAACCCC GAACACCTGT TGGGAGTAGG AAAGCCAGCC GTAACATATT TTTAGAGGAT 
GATTTGGCCT GGCAAAGTTT GATTCATCCA GATTCCTCAA ATACTCCTCT TTCAACAAGA 
CTTGTATCTG TGCAAGAGGA TGCTGGGAAA TCTCCTGCTC GAAATAGATC AGCCAGCATT 
ACTAACCTGT CACTGGATAG ATCTGGTTCT CCTATGGTAC CTTCATATGA GACATCTGTC 
AGTCCCCAGG CTAACCGAAC ATATGTTAGG ACAGAGACCA CTGAGGATGA ACGCAAAATT 
CTTCTGGACA GTGTGCAGTT AAAAGACCTG TGGAAAAAAA TCTGCCATCA CAGCAGTGGA 
ATGGAGTTTC AGGATCACCG CTACTGGTTG AGAACGCATC CCAACTGCAT TGTAGGAAAG 
GAATTAGTCA ACTGGCTAAT CCGAAATGGG CATATTGCCA CAAGGGCACA AGCTATAGCA 
ATTGGACAAG CAATGGTTGA TGGACGTTGG CTGGATTGTG TTAGTCATCA CGACCAGCTT 
TTCAGAGATG AGTATGCGCT GTATAGACCA CTGCAGAGTA CAGAATTTTC TGAGACGCCT 
TCTCCCGACA GTGACTCAGT GAACTCCGTG GAAGGACACT CTGAGCCATC CTGGTTTAAA 
GACATAAAGT TTGATGACAG TGACACAGAA CAGATAGCTG AAGAAGGTGA CGATAATTTG 
GCTAATTCTG CCAGTCCTAG CAAGCGCACA TCAGTCAGCA GTTTCCAGTC CACAGTGGAC 
AGTGACTCAG CCGCTTCTAT CAGCCTGAAC GTGGAGCTGG ACAACGTGAA CTTCCATATC 
AAGAAGCCCT CCAAGTACCC ACATGTGCCC CCTCACCCTG CTGACCAAAA AGAGTATTTG 
ATTTCTGACA CTGGAGGACA ACAGCTCTCA ATAAGTGACG CTTTCATCAA AGAATCCTTA 
TTTAATCGCC GAGTAGAGGA AAAATCCAAA GAGCTGCCTT TCACACCTTT GGGCTGGCAT 
CATAACAACC TGGAGCTCCT GAGGGAGGAG AATGGGGAGA AACAAGCCAT GGAGAGGTTG 
CTTTCAGCTA ATCATAACCA CATGATGGCA CTACTCCAGC AGTTGCTCCA TAGTGACTCA 
CTGTCATCAT CTTGGAGGGA CATCATCGTG TCATTGGTCT GCCAGGTTGT TCAGACAGTC 
CGACCTGATG TCAAGAACCA GGATGATGAC ATGGATATCC GTCAGTTTGT CCACATCAAA 
AAAATCCCAG GTGGAAAGAA GTTTGATTCT GTGGTTGTCA ATGGCTTTGT TTGTACCAAG 
AACATTGCAC ATAAAAAGAT GAGTTCTTGT ATTAAAAACC CCAAAATTCT TCTGTTGAAG 
TGTTCCATTG AGTATCTCTA CAGAGAAGAA ACTAAGTTTA CTTGCATTGA TCCTATTGTG 
CTTCAGGAAA GGGAATTCTT GAAGAATTAT GTCCAGCGAA TAGTTGATGT TCGACCCACC 
TTGGTTCTTG TTGAGAAAAC AGTGTCTCGG ATTGCCCAGG ACATGTTATT GGAACATGGC 
ATTACTTTGG TCATTAATGT AAAGTCACAA GTTTTGGAAC GAATCAGTCG AATGACCCAA 
GGTGATTTAG TGATGTCAAT GGACCAGCTG CTTACGAAAC CACACCTGGG CACTTGTCAC 
AAATTTTATA TGCAGATATT TCAGTTGCCT AATGAACAAA CCAAGACACT GATGTTTTTT 
GAAGGTTGTC CACAGCACCT AGGCTGTACA ATCAAGCTAA GAGGAGGCTC TGATTATGAG 
CTGGCTCGAG TTAAGGAGAT CCTAATATTT ATGATCTGTG TTGCTTATCA TTCTCAACTA 
GAAATATCCT TTCTCATGGA TGAATTTGCT ATGCCTCCCA CATTAATGCA AAACCCTTCA 
TTCCATTCCC TGATTGAGGG ACGAGGGCAT GAGGGGGCTG TCCAAGAGCA GTACGGTGGA 
GGTTCCATCC CCTGGGATCC TGACATCCCT CCTGAGTCTC TGCCCTGTGA TGATAGCAGT 
TTGCTGGAAT TGAGGATTGT GTTTGAGAAG GGTGAGCAGG AAAATAAAAA TCTTCCGCAG 
GCTGTTGCCT CTGTGAAGCA TCAAGAACAT AGCACAACAG CTTGCCCGGC GGGTCTCCCT 
TGTGCTTTCT TTGCACCTGT ACCGGAATCA TTGTTGCCAC TCCCTGTGGA TGACCAACAA 
GATGCTTTAG GCAGCGAGCA GCCAGAGACT TTGCAGCAAA CAGTTGTGCT GCAGGATCCC 
AAAAGCCAGA TAAGAGCCTT TAGAGACCCT CTACAGGATG ACACTGGATT ATATGTTACT 
GAGGAAGTCA CCTCCTCTGA AGATAAACGA AAGACTTATT CTTTGGCCTT TAAGCAGGAA 
TTAAAAGATG TGATCCTCTG TATCTCCCCA GTAATCACAT TCCGAGAACC CTTTCTTTTA 
ACTGAAAAGG GGATGAGATG CTCTACCCGA GATTATTTTG CAGAGCAGGT TTACTGGTCT 
CCTCTCCTCA ATAAAGAATT CAAAGAAATG GAGAACAGGA GGAAGAAACA GCTGCTCAGG 
GATCTCTCTG GACTTCAGGG CATGAATGGA AGTATTCAGG CCAAGTCTAT TCAAGTCTTA 
CCCTCACATG AGCTAGTGAG CACTAGAATT GCTGAGCATC TGGGCGATAG CCAGAGCTTG 
GGTAGAATGC TGGCCGATTA TCGAGCCAGA GGAGGAAGAA TTCAGCCCAA AAATTCAGAC 
CCTTTTGCTC ATTCAAAGGA TGCATCAAGT ACTTCAAGTG GCCAATCAGG AAGCAAAAAT 
GAGGGTGATG AAGAGAGAGG GCTTATTCTG AGTGATGCTG TGTGGTCAAC AAAGGTGGAC 
TGTCTGAATC CCATTAATCA CCAGAGACTT TGTGTGCTCT TCAGCAGCTC TTCTGCCCAG 
TCCAGCAATG CTCCTAGTGC CTGTGTCAGT CCTTGGATTG TAACAATGGA ATTTTATGGA 
AAGAATGATC TTACATTAGG AATATTTTTA GAGAGATACT GTTTCAGGCC TTCTTATCAG 
TGTCCAAGCA TGTTCTGTGA TACCCCCATG GTACATCATA TTCGGCGCTT TGTTCATGGC 
CAAGGCTGTG TGCAGATAAT CCTGAAGGAG TTGGATTCTC CAGTACCTGG ATATCAGCAT 
ACAATTCTTA CATATTCCTG GTGTAGAATC TGCAAACAGG TAACACCAGT TGTTGCTCTT 
TCCAATGAGT CCTGGTCTAT GTCATTTGCA AAATACCTTG AACTTAGGTT TTATGGGCAC 
CAGTATACTC GCAGAGCCAA CGCTGAGCCC TGTGGTCACT CCATCCATCA TGATTATCAC 
CAGTATTTCT CCTATAACCA GATGGTGGCG TCTTTCAGTT ATTCTCCCAT TCGGCTTCTT 
GAAGTATGTG TTCCACTCCC CAAAATATTC ATTAAGCGTC AGGCCCCATT AAAAGTGTCC 
CTTCTTCAGG ATCTGAAGGA CTTCTTTCAA AAAGTTTCAC AGGTATATGT TGCCATTGAT 
GAAAGACTTG CATCTTTGAA AACTGATACA TTTAGTAAAA CAAGAGAGGA AAAAATGGAA 
GATATTTTTG CACAGAAAGA GATGGAAGAA GGTGAGTTCA AGAACTGGAT TGAGAAGATG 
CAAGCAAGGC TCATGTCTTC CTCTGTAGAT ACCCCTCAGC AACTGCAGTC GGTCTTTGAG 
TCACTCATTG CCAAGAAACA AAGTCTCTGT GAAGTGCTGC AAGCTTGGAA TAACAGGTTG 
CAGGACCTTT TCCAACAGGA AAAGGGTAGA AAGAGACCTT CAGTTCCTCC AAGTCCTGGA 
AGACTGAGAC AAGGGGAAGA AAGCAAGATA AGTGCGATGG ATGCATCTCC ACGGAATATT 
TCTCCAGGAC TTCAGAATGG AGAAAAAGAG GATCGCTTCT TAACAACTTT GTCCAGCCAG 
AGCTCCACCA GTTCTACTCA TCTCCAATTG CCTACGCCAC CTGAAGTCAT GTCTGAACAG 
TCAGTGGGAG GGCCCCCTGA GCTAGATACA GCCAGCAGTT CCGAAGATGT GTTTGATGGG 
CATTTGCTGG GATCCACAGA CAGCCAAGTG AAGGAAAAGT CAACCATGAA AGCCATCTTT 
GCAAATTTGC TTCCAGGAAA TAGCTATAAT CCTATTCCAT TTCCTTTTGA TCCAGATAAA 
CACTACTTAA TGTATGAACA TGAACGAGTG CCCATTGCAG TCTGCGAGAA GGAACCCAGC 
TCCATCATTG CTTTTGCTCT CAGTTGTAAA GAATACCGAA ATGCCTTAGA GGAATTGTCT 
AAAGCGACTC AGTGGAACAG TGCCGAAGAA GGGCTTCCAA CAAATAGTAC TTCAGATAGC 
AGACCAAAGA GTAGCAGCCC TATCAGATTA CCTGAAATGA GTGGAGGACA GACAAATCGT 
ACAACAGAAA CAGAACCACA ACCAACCAAA AAGGCTTCTG GAATGTTGTC CTTCTTCAGA 
GGGACAGCAG GGAAAAGCCC CGATCTCTCT TCCCAGAAGA GAGAGACCTT ACGTGGAGCA 
GATAGTGCTT ACTACCAGGT TGGGCAGACG GGCAAGGAGG GGACCGAGAA TCAAGGCGTT 
GAGCCTCAAG ATGAAGTAGA TGGAGGAGAT ACACAAAAGA AGCAACTCAT AAATCCTCAT 
GTGGAACTTC AATTTTCAGA TGCTAATGCC AAGTTTTACT GTCGGCTCTA CTATGCGGGA 
GAGTTTCATA AGATGCGTGA AGTGATTCTG GACAGCAGTG AAGAAGATTT CATTCGTTCC 
CTCTCCCACT CATCACCCTG GCAGGCCCGG GGAGGCAAAT CAGGAGCTGC CTTCTATGCA 
ACTGAGGATG ATAGATTTAT TTTGAAGCAA ATGCCTCGTC TGGAAGTCCA GTCCTTCCTC 
GACTTTGCAC CACATTACTT CAATTATATT ACAAATGCTG TTCAACAAAA GAGGCCCACG 
GCGTTGGCCA AAATTCTTGG AGTTTACAGA ATTGGTTATA AGAACTCTCA GAACAACACT 
GAGAAGAAGT TAGATCTCCT TGTCATGGAA AATCTTTTCT ACGGGAGAAA GATGGCACAG 
GTTTTTGATT TGAAGGGCTC TCTTAGGAAT CGGAATGTAA AAACTGACAC TGGAAAAGAG 
AGTTGTGATG TGGTCCTGCT AGATGAAAAT CTCCTAAAGA TGGTTCGAGA CAACCCTCTA 
TATATTCGTT CTCATTCCAA AGCTGTGCTG AGAACCTCGA TCCATAGTGA CTCCCATTTC 
CTTTCTAGCC ACCTCATTAT AGATTATTCT TTGCTGGTTG GGCGAGATGA TACTAGCAAT 
GAGCTAGTAG TTGGAATTAT AGATTATATT CGAACATTTA CATGGGACAA AAAGCTTGAG 
ATGGTTGTGA AATCAACAGG AATTTTAGGT GGACAAGGTA AAATGCCAAC AGTGGTGTCT 
CCGGAGTTGT ACAGGACTAG GTTTTGTGAG GCAATGGACA AGTATTTCCT AATGGTACCA 
GACCACTGGA CAGGCTTGGG TCTGAATTGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011509080.1
CDS	182..6514
Translation	MATDDKTSPTLDSANDLPRSPTSPSHLTHFKPLTPDQDEPPFKSAYSSFVNLFRFNKERAEGGQGEQQPLSGSWTSPQLPSRTQSVRSPTPYKKQLNEELQRRSSALADNSLQHPQENTDTRRKAEPTFGGHDPRTAVQLRSLSTVLKRLKEIMEGKSQDSDLKQYWMPDSQCKECYDCSEKFTTFRRRHHCRLCGQIFCSRCCNQEIPGKFMGYTGDLRACTYCRKIALSYAHSTDSNSIGEDLNALSDSACSVSVLDPSEPRTPVGSRKASRNIFLEDDLAWQSLIHPDSSNTPLSTRLVSVQEDAGKSPARNRSASITNLSLDRSGSPMVPSYETSVSPQANRTYVRTETTEDERKILLDSVQLKDLWKKICHHSSGMEFQDHRYWLRTHPNCIVGKELVNWLIRNGHIATRAQAIAIGQAMVDGRWLDCVSHHDQLFRDEYALYRPLQSTEFSETPSPDSDSVNSVEGHSEPSWFKDIKFDDSDTEQIAEEGDDNLANSASPSKRTSVSSFQSTVDSDSAASISLNVELDNVNFHIKKPSKYPHVPPHPADQKEYLISDTGGQQLSISDAFIKESLFNRRVEEKSKELPFTPLGWHHNNLELLREENGEKQAMERLLSANHNHMMALLQQLLHSDSLSSSWRDIIVSLVCQVVQTVRPDVKNQDDDMDIRQFVHIKKIPGGKKFDSVVVNGFVCTKNIAHKKMSSCIKNPKILLLKCSIEYLYREETKFTCIDPIVLQEREFLKNYVQRIVDVRPTLVLVEKTVSRIAQDMLLEHGITLVINVKSQVLERISRMTQGDLVMSMDQLLTKPHLGTCHKFYMQIFQLPNEQTKTLMFFEGCPQHLGCTIKLRGGSDYELARVKEILIFMICVAYHSQLEISFLMDEFAMPPTLMQNPSFHSLIEGRGHEGAVQEQYGGGSIPWDPDIPPESLPCDDSSLLELRIVFEKGEQENKNLPQAVASVKHQEHSTTACPAGLPCAFFAPVPESLLPLPVDDQQDALGSEQPETLQQTVVLQDPKSQIRAFRDPLQDDTGLYVTEEVTSSEDKRKTYSLAFKQELKDVILCISPVITFREPFLLTEKGMRCSTRDYFAEQVYWSPLLNKEFKEMENRRKKQLLRDLSGLQGMNGSIQAKSIQVLPSHELVSTRIAEHLGDSQSLGRMLADYRARGGRIQPKNSDPFAHSKDASSTSSGQSGSKNEGDEERGLILSDAVWSTKVDCLNPINHQRLCVLFSSSSAQSSNAPSACVSPWIVTMEFYGKNDLTLGIFLERYCFRPSYQCPSMFCDTPMVHHIRRFVHGQGCVQIILKELDSPVPGYQHTILTYSWCRICKQVTPVVALSNESWSMSFAKYLELRFYGHQYTRRANAEPCGHSIHHDYHQYFSYNQMVASFSYSPIRLLEVCVPLPKIFIKRQAPLKVSLLQDLKDFFQKVSQVYVAIDERLASLKTDTFSKTREEKMEDIFAQKEMEEGEFKNWIEKMQARLMSSSVDTPQQLQSVFESLIAKKQSLCEVLQAWNNRLQDLFQQEKGRKRPSVPPSPGRLRQGEESKISAMDASPRNISPGLQNGEKEDRFLTTLSSQSSTSSTHLQLPTPPEVMSEQSVGGPPELDTASSSEDVFDGHLLGSTDSQVKEKSTMKAIFANLLPGNSYNPIPFPFDPDKHYLMYEHERVPIAVCEKEPSSIIAFALSCKEYRNALEELSKATQWNSAEEGLPTNSTSDSRPKSSSPIRLPEMSGGQTNRTTETEPQPTKKASGMLSFFRGTAGKSPDLSSQKRETLRGADSAYYQVGQTGKEGTENQGVEPQDEVDGGDTQKKQLINPHVELQFSDANAKFYCRLYYAGEFHKMREVILDSSEEDFIRSLSHSSPWQARGGKSGAAFYATEDDRFILKQMPRLEVQSFLDFAPHYFNYITNAVQQKRPTALAKILGVYRIGYKNSQNNTEKKLDLLVMENLFYGRKMAQVFDLKGSLRNRNVKTDTGKESCDVVLLDENLLKMVRDNPLYIRSHSKAVLRTSIHSDSHFLSSHLIIDYSLLVGRDDTSNELVVGIIDYIRTFTWDKKLEMVVKSTGILGGQGKMPTVVSPELYRTRFCEAMDKYFLMVPDHWTGLGLNC

Target ORF information:

RefSeq Version	XM_011510778.3
Organism	Homo sapiens(human)
Definition	Homo sapiens phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011510778.3

ORF Insert Sequence:

ATGGCCACAG ATGATAAGAC GTCCCCAACA CTGGACTCTG CTAATGATTT GCCTCGATCT 
CCTACTAGTC CTTCTCATCT CACACACTTT AAACCTTTGA CTCCTGATCA AGATGAGCCC 
CCTTTTAAAT CAGCTTATAG TTCTTTTGTA AATCTCTTTC GTTTTAACAA AGAGAGAGCA 
GAAGGAGGCC AGGGAGAACA GCAGCCTTTG AGTGGAAGTT GGACCAGCCC TCAGCTCCCT 
TCGAGGACAC AGTCTGTTAG GTCACCCACA CCTTATAAAA AGCAGCTTAA TGAGGAACTC 
CAGCGGCGCT CTTCAGCATT AGCAGACAAC AGTTTGCAAC ATCCCCAGGA GAACACAGAC 
ACAAGAAGGA AAGCAGAACC TACCTTTGGA GGTCATGACC CTCGTACAGC TGTTCAGCTT 
CGAAGCCTCA GCACAGTATT AAAACGCCTC AAGGAAATCA TGGAGGGGAA AAGCCAGGAT 
AGTGACCTGA AACAATACTG GATGCCAGAT AGCCAATGTA AAGAGTGCTA TGACTGTAGT 
GAGAAATTTA CAACCTTTAG GCGCAGACAC CATTGCCGAC TATGTGGGCA GATTTTCTGC 
AGTCGTTGCT GTAATCAAGA AATCCCTGGA AAATTTATGG GCTATACAGG AGACCTCCGA 
GCTTGCACAT ATTGTAGAAA AATAGCCTTA AGTTATGCTC ATTCCACAGA CAGTAATTCT 
ATTGGGGAAG ACTTGAATGC TCTTTCAGAT TCTGCTTGCT CTGTGTCTGT GCTTGATCCA 
AGTGAACCCC GAACACCTGT TGGGAGTAGG AAAGCCAGCC GTAACATATT TTTAGAGGAT 
GATTTGGCCT GGCAAAGTTT GATTCATCCA GATTCCTCAA ATACTCCTCT TTCAACAAGA 
CTTGTATCTG TGCAAGAGGA TGCTGGGAAA TCTCCTGCTC GAAATAGATC AGCCAGCATT 
ACTAACCTGT CACTGGATAG ATCTGGTTCT CCTATGGTAC CTTCATATGA GACATCTGTC 
AGTCCCCAGG CTAACCGAAC ATATGTTAGG ACAGAGACCA CTGAGGATGA ACGCAAAATT 
CTTCTGGACA GTGTGCAGTT AAAAGACCTG TGGAAAAAAA TCTGCCATCA CAGCAGTGGA 
ATGGAGTTTC AGGATCACCG CTACTGGTTG AGAACGCATC CCAACTGCAT TGTAGGAAAG 
GAATTAGTCA ACTGGCTAAT CCGAAATGGG CATATTGCCA CAAGGGCACA AGCTATAGCA 
ATTGGACAAG CAATGGTTGA TGGACGTTGG CTGGATTGTG TTAGTCATCA CGACCAGCTT 
TTCAGAGATG AGTATGCGCT GTATAGACCA CTGCAGAGTA CAGAATTTTC TGAGACGCCT 
TCTCCCGACA GTGACTCAGT GAACTCCGTG GAAGGACACT CTGAGCCATC CTGGTTTAAA 
GACATAAAGT TTGATGACAG TGACACAGAA CAGATAGCTG AAGAAGGTGA CGATAATTTG 
GCTAATTCTG CCAGTCCTAG CAAGCGCACA TCAGTCAGCA GTTTCCAGTC CACAGTGGAC 
AGTGACTCAG CCGCTTCTAT CAGCCTGAAC GTGGAGCTGG ACAACGTGAA CTTCCATATC 
AAGAAGCCCT CCAAGTACCC ACATGTGCCC CCTCACCCTG CTGACCAAAA AGAGTATTTG 
ATTTCTGACA CTGGAGGACA ACAGCTCTCA ATAAGTGACG CTTTCATCAA AGAATCCTTA 
TTTAATCGCC GAGTAGAGGA AAAATCCAAA GAGCTGCCTT TCACACCTTT GGGCTGGCAT 
CATAACAACC TGGAGCTCCT GAGGGAGGAG AATGGGGAGA AACAAGCCAT GGAGAGGTTG 
CTTTCAGCTA ATCATAACCA CATGATGGCA CTACTCCAGC AGTTGCTCCA TAGTGACTCA 
CTGTCATCAT CTTGGAGGGA CATCATCGTG TCATTGGTCT GCCAGGTTGT TCAGACAGTC 
CGACCTGATG TCAAGAACCA GGATGATGAC ATGGATATCC GTCAGTTTGT CCACATCAAA 
AAAATCCCAG GTGGAAAGAA GTTTGATTCT GTGGTTGTCA ATGGCTTTGT TTGTACCAAG 
AACATTGCAC ATAAAAAGAT GAGTTCTTGT ATTAAAAACC CCAAAATTCT TCTGTTGAAG 
TGTTCCATTG AGTATCTCTA CAGAGAAGAA ACTAAGTTTA CTTGCATTGA TCCTATTGTG 
CTTCAGGAAA GGGAATTCTT GAAGAATTAT GTCCAGCGAA TAGTTGATGT TCGACCCACC 
TTGGTTCTTG TTGAGAAAAC AGTGTCTCGG ATTGCCCAGG ACATGTTATT GGAACATGGC 
ATTACTTTGG TCATTAATGT AAAGTCACAA GTTTTGGAAC GAATCAGTCG AATGACCCAA 
GGTGATTTAG TGATGTCAAT GGACCAGCTG CTTACGAAAC CACACCTGGG CACTTGTCAC 
AAATTTTATA TGCAGATATT TCAGTTGCCT AATGAACAAA CCAAGACACT GATGTTTTTT 
GAAGGTTGTC CACAGCACCT AGGCTGTACA ATCAAGCTAA GAGGAGGCTC TGATTATGAG 
CTGGCTCGAG TTAAGGAGAT CCTAATATTT ATGATCTGTG TTGCTTATCA TTCTCAACTA 
GAAATATCCT TTCTCATGGA TGAATTTGCT ATGCCTCCCA CATTAATGCA AAACCCTTCA 
TTCCATTCCC TGATTGAGGG ACGAGGGCAT GAGGGGGCTG TCCAAGAGCA GTACGGTGGA 
GGTTCCATCC CCTGGGATCC TGACATCCCT CCTGAGTCTC TGCCCTGTGA TGATAGCAGT 
TTGCTGGAAT TGAGGATTGT GTTTGAGAAG GGTGAGCAGG AAAATAAAAA TCTTCCGCAG 
GCTGTTGCCT CTGTGAAGCA TCAAGAACAT AGCACAACAG CTTGCCCGGC GGGTCTCCCT 
TGTGCTTTCT TTGCACCTGT ACCGGAATCA TTGTTGCCAC TCCCTGTGGA TGACCAACAA 
GATGCTTTAG GCAGCGAGCA GCCAGAGACT TTGCAGCAAA CAGTTGTGCT GCAGGATCCC 
AAAAGCCAGA TAAGAGCCTT TAGAGACCCT CTACAGGATG ACACTGGATT ATATGTTACT 
GAGGAAGTCA CCTCCTCTGA AGATAAACGA AAGACTTATT CTTTGGCCTT TAAGCAGGAA 
TTAAAAGATG TGATCCTCTG TATCTCCCCA GTAATCACAT TCCGAGAACC CTTTCTTTTA 
ACTGAAAAGG GGATGAGATG CTCTACCCGA GATTATTTTG CAGAGCAGGT TTACTGGTCT 
CCTCTCCTCA ATAAAGAATT CAAAGAAATG GAGAACAGGA GGAAGAAACA GCTGCTCAGG 
GATCTCTCTG GACTTCAGGG CATGAATGGA AGTATTCAGG CCAAGTCTAT TCAAGTCTTA 
CCCTCACATG AGCTAGTGAG CACTAGAATT GCTGAGCATC TGGGCGATAG CCAGAGCTTG 
GGTAGAATGC TGGCCGATTA TCGAGCCAGA GGAGGAAGAA TTCAGCCCAA AAATTCAGAC 
CCTTTTGCTC ATTCAAAGGA TGCATCAAGT ACTTCAAGTG GCCAATCAGG AAGCAAAAAT 
GAGGGTGATG AAGAGAGAGG GCTTATTCTG AGTGATGCTG TGTGGTCAAC AAAGGTGGAC 
TGTCTGAATC CCATTAATCA CCAGAGACTT TGTGTGCTCT TCAGCAGCTC TTCTGCCCAG 
TCCAGCAATG CTCCTAGTGC CTGTGTCAGT CCTTGGATTG TAACAATGGA ATTTTATGGA 
AAGAATGATC TTACATTAGG AATATTTTTA GAGAGATACT GTTTCAGGCC TTCTTATCAG 
TGTCCAAGCA TGTTCTGTGA TACCCCCATG GTACATCATA TTCGGCGCTT TGTTCATGGC 
CAAGGCTGTG TGCAGATAAT CCTGAAGGAG TTGGATTCTC CAGTACCTGG ATATCAGCAT 
ACAATTCTTA CATATTCCTG GTGTAGAATC TGCAAACAGG TAACACCAGT TGTTGCTCTT 
TCCAATGAGT CCTGGTCTAT GTCATTTGCA AAATACCTTG AACTTAGGTT TTATGGGCAC 
CAGTATACTC GCAGAGCCAA CGCTGAGCCC TGTGGTCACT CCATCCATCA TGATTATCAC 
CAGTATTTCT CCTATAACCA GATGGTGGCG TCTTTCAGTT ATTCTCCCAT TCGGCTTCTT 
GAAGTATGTG TTCCACTCCC CAAAATATTC ATTAAGCGTC AGGCCCCATT AAAAGTGTCC 
CTTCTTCAGG ATCTGAAGGA CTTCTTTCAA AAAGTTTCAC AGGTATATGT TGCCATTGAT 
GAAAGACTTG CATCTTTGAA AACTGATACA TTTAGTAAAA CAAGAGAGGA AAAAATGGAA 
GATATTTTTG CACAGAAAGA GATGGAAGAA GGTGAGTTCA AGAACTGGAT TGAGAAGATG 
CAAGCAAGGC TCATGTCTTC CTCTGTAGAT ACCCCTCAGC AACTGCAGTC GGTCTTTGAG 
TCACTCATTG CCAAGAAACA AAGTCTCTGT GAAGTGCTGC AAGCTTGGAA TAACAGGTTG 
CAGGACCTTT TCCAACAGGA AAAGGGTAGA AAGAGACCTT CAGTTCCTCC AAGTCCTGGA 
AGACTGAGAC AAGGGGAAGA AAGCAAGATA AGTGCGATGG ATGCATCTCC ACGGAATATT 
TCTCCAGGAC TTCAGAATGG AGAAAAAGAG GATCGCTTCT TAACAACTTT GTCCAGCCAG 
AGCTCCACCA GTTCTACTCA TCTCCAATTG CCTACGCCAC CTGAAGTCAT GTCTGAACAG 
TCAGTGGGAG GGCCCCCTGA GCTAGATACA GCCAGCAGTT CCGAAGATGT GTTTGATGGG 
CATTTGCTGG GATCCACAGA CAGCCAAGTG AAGGAAAAGT CAACCATGAA AGCCATCTTT 
GCAAATTTGC TTCCAGGAAA TAGCTATAAT CCTATTCCAT TTCCTTTTGA TCCAGATAAA 
CACTACTTAA TGTATGAACA TGAACGAGTG CCCATTGCAG TCTGCGAGAA GGAACCCAGC 
TCCATCATTG CTTTTGCTCT CAGTTGTAAA GAATACCGAA ATGCCTTAGA GGAATTGTCT 
AAAGCGACTC AGTGGAACAG TGCCGAAGAA GGGCTTCCAA CAAATAGTAC TTCAGATAGC 
AGACCAAAGA GTAGCAGCCC TATCAGATTA CCTGAAATGA GTGGAGGACA GACAAATCGT 
ACAACAGAAA CAGAACCACA ACCAACCAAA AAGGCTTCTG GAATGTTGTC CTTCTTCAGA 
GGGACAGCAG GGAAAAGCCC CGATCTCTCT TCCCAGAAGA GAGAGACCTT ACGTGGAGCA 
GATAGTGCTT ACTACCAGGT TGGGCAGACG GGCAAGGAGG GGACCGAGAA TCAAGGCGTT 
GAGCCTCAAG ATGAAGTAGA TGGAGGAGAT ACACAAAAGA AGCAACTCAT AAATCCTCAT 
GTGGAACTTC AATTTTCAGA TGCTAATGCC AAGTTTTACT GTCGGCTCTA CTATGCGGGA 
GAGTTTCATA AGATGCGTGA AGTGATTCTG GACAGCAGTG AAGAAGATTT CATTCGTTCC 
CTCTCCCACT CATCACCCTG GCAGGCCCGG GGAGGCAAAT CAGGAGCTGC CTTCTATGCA 
ACTGAGGATG ATAGATTTAT TTTGAAGCAA ATGCCTCGTC TGGAAGTCCA GTCCTTCCTC 
GACTTTGCAC CACATTACTT CAATTATATT ACAAATGCTG TTCAACAAAA GAGGCCCACG 
GCGTTGGCCA AAATTCTTGG AGTTTACAGA ATTGGTTATA AGAACTCTCA GAACAACACT 
GAGAAGAAGT TAGATCTCCT TGTCATGGAA AATCTTTTCT ACGGGAGAAA GATGGCACAG 
GTTTTTGATT TGAAGGGCTC TCTTAGGAAT CGGAATGTAA AAACTGACAC TGGAAAAGAG 
AGTTGTGATG TGGTCCTGCT AGATGAAAAT CTCCTAAAGA TGGTTCGAGA CAACCCTCTA 
TATATTCGTT CTCATTCCAA AGCTGTGCTG AGAACCTCGA TCCATAGTGA CTCCCATTTC 
CTTTCTAGCC ACCTCATTAT AGATTATTCT TTGCTGGTTG GGCGAGATGA TACTAGCAAT 
GAGCTAGTAG TTGGAATTAT AGATTATATT CGAACATTTA CATGGGACAA AAAGCTTGAG 
ATGGTTGTGA AATCAACAGG AATTTTAGGT GGACAAGGTA AAATGCCAAC AGTGGTGTCT 
CCGGAGTTGT ACAGGACTAG GTTTTGTGAG GCAATGGACA AGTATTTCCT AATGGTACCA 
GACCACTGGA CAGGCTTGGG TCTGAATTGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PIKFYVE ( XM_011510779.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011509081.1 Homo sapiens phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X2, mRNA.

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CloneID	OHu77661
Clone ID Related Accession (Same CDS sequence)	XM_011510778.2 , XM_011510778.3 , XM_011510779.2
Accession Version	XM_011510779.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6333bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	1-phosphatidylinositol 3-phosphate 5-kinase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011510779.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG ATGATAAGAC GTCCCCAACA CTGGACTCTG CTAATGATTT GCCTCGATCT 
CCTACTAGTC CTTCTCATCT CACACACTTT AAACCTTTGA CTCCTGATCA AGATGAGCCC 
CCTTTTAAAT CAGCTTATAG TTCTTTTGTA AATCTCTTTC GTTTTAACAA AGAGAGAGCA 
GAAGGAGGCC AGGGAGAACA GCAGCCTTTG AGTGGAAGTT GGACCAGCCC TCAGCTCCCT 
TCGAGGACAC AGTCTGTTAG GTCACCCACA CCTTATAAAA AGCAGCTTAA TGAGGAACTC 
CAGCGGCGCT CTTCAGCATT AGCAGACAAC AGTTTGCAAC ATCCCCAGGA GAACACAGAC 
ACAAGAAGGA AAGCAGAACC TACCTTTGGA GGTCATGACC CTCGTACAGC TGTTCAGCTT 
CGAAGCCTCA GCACAGTATT AAAACGCCTC AAGGAAATCA TGGAGGGGAA AAGCCAGGAT 
AGTGACCTGA AACAATACTG GATGCCAGAT AGCCAATGTA AAGAGTGCTA TGACTGTAGT 
GAGAAATTTA CAACCTTTAG GCGCAGACAC CATTGCCGAC TATGTGGGCA GATTTTCTGC 
AGTCGTTGCT GTAATCAAGA AATCCCTGGA AAATTTATGG GCTATACAGG AGACCTCCGA 
GCTTGCACAT ATTGTAGAAA AATAGCCTTA AGTTATGCTC ATTCCACAGA CAGTAATTCT 
ATTGGGGAAG ACTTGAATGC TCTTTCAGAT TCTGCTTGCT CTGTGTCTGT GCTTGATCCA 
AGTGAACCCC GAACACCTGT TGGGAGTAGG AAAGCCAGCC GTAACATATT TTTAGAGGAT 
GATTTGGCCT GGCAAAGTTT GATTCATCCA GATTCCTCAA ATACTCCTCT TTCAACAAGA 
CTTGTATCTG TGCAAGAGGA TGCTGGGAAA TCTCCTGCTC GAAATAGATC AGCCAGCATT 
ACTAACCTGT CACTGGATAG ATCTGGTTCT CCTATGGTAC CTTCATATGA GACATCTGTC 
AGTCCCCAGG CTAACCGAAC ATATGTTAGG ACAGAGACCA CTGAGGATGA ACGCAAAATT 
CTTCTGGACA GTGTGCAGTT AAAAGACCTG TGGAAAAAAA TCTGCCATCA CAGCAGTGGA 
ATGGAGTTTC AGGATCACCG CTACTGGTTG AGAACGCATC CCAACTGCAT TGTAGGAAAG 
GAATTAGTCA ACTGGCTAAT CCGAAATGGG CATATTGCCA CAAGGGCACA AGCTATAGCA 
ATTGGACAAG CAATGGTTGA TGGACGTTGG CTGGATTGTG TTAGTCATCA CGACCAGCTT 
TTCAGAGATG AGTATGCGCT GTATAGACCA CTGCAGAGTA CAGAATTTTC TGAGACGCCT 
TCTCCCGACA GTGACTCAGT GAACTCCGTG GAAGGACACT CTGAGCCATC CTGGTTTAAA 
GACATAAAGT TTGATGACAG TGACACAGAA CAGATAGCTG AAGAAGGTGA CGATAATTTG 
GCTAATTCTG CCAGTCCTAG CAAGCGCACA TCAGTCAGCA GTTTCCAGTC CACAGTGGAC 
AGTGACTCAG CCGCTTCTAT CAGCCTGAAC GTGGAGCTGG ACAACGTGAA CTTCCATATC 
AAGAAGCCCT CCAAGTACCC ACATGTGCCC CCTCACCCTG CTGACCAAAA AGAGTATTTG 
ATTTCTGACA CTGGAGGACA ACAGCTCTCA ATAAGTGACG CTTTCATCAA AGAATCCTTA 
TTTAATCGCC GAGTAGAGGA AAAATCCAAA GAGCTGCCTT TCACACCTTT GGGCTGGCAT 
CATAACAACC TGGAGCTCCT GAGGGAGGAG AATGGGGAGA AACAAGCCAT GGAGAGGTTG 
CTTTCAGCTA ATCATAACCA CATGATGGCA CTACTCCAGC AGTTGCTCCA TAGTGACTCA 
CTGTCATCAT CTTGGAGGGA CATCATCGTG TCATTGGTCT GCCAGGTTGT TCAGACAGTC 
CGACCTGATG TCAAGAACCA GGATGATGAC ATGGATATCC GTCAGTTTGT CCACATCAAA 
AAAATCCCAG GTGGAAAGAA GTTTGATTCT GTGGTTGTCA ATGGCTTTGT TTGTACCAAG 
AACATTGCAC ATAAAAAGAT GAGTTCTTGT ATTAAAAACC CCAAAATTCT TCTGTTGAAG 
TGTTCCATTG AGTATCTCTA CAGAGAAGAA ACTAAGTTTA CTTGCATTGA TCCTATTGTG 
CTTCAGGAAA GGGAATTCTT GAAGAATTAT GTCCAGCGAA TAGTTGATGT TCGACCCACC 
TTGGTTCTTG TTGAGAAAAC AGTGTCTCGG ATTGCCCAGG ACATGTTATT GGAACATGGC 
ATTACTTTGG TCATTAATGT AAAGTCACAA GTTTTGGAAC GAATCAGTCG AATGACCCAA 
GGTGATTTAG TGATGTCAAT GGACCAGCTG CTTACGAAAC CACACCTGGG CACTTGTCAC 
AAATTTTATA TGCAGATATT TCAGTTGCCT AATGAACAAA CCAAGACACT GATGTTTTTT 
GAAGGTTGTC CACAGCACCT AGGCTGTACA ATCAAGCTAA GAGGAGGCTC TGATTATGAG 
CTGGCTCGAG TTAAGGAGAT CCTAATATTT ATGATCTGTG TTGCTTATCA TTCTCAACTA 
GAAATATCCT TTCTCATGGA TGAATTTGCT ATGCCTCCCA CATTAATGCA AAACCCTTCA 
TTCCATTCCC TGATTGAGGG ACGAGGGCAT GAGGGGGCTG TCCAAGAGCA GTACGGTGGA 
GGTTCCATCC CCTGGGATCC TGACATCCCT CCTGAGTCTC TGCCCTGTGA TGATAGCAGT 
TTGCTGGAAT TGAGGATTGT GTTTGAGAAG GGTGAGCAGG AAAATAAAAA TCTTCCGCAG 
GCTGTTGCCT CTGTGAAGCA TCAAGAACAT AGCACAACAG CTTGCCCGGC GGGTCTCCCT 
TGTGCTTTCT TTGCACCTGT ACCGGAATCA TTGTTGCCAC TCCCTGTGGA TGACCAACAA 
GATGCTTTAG GCAGCGAGCA GCCAGAGACT TTGCAGCAAA CAGTTGTGCT GCAGGATCCC 
AAAAGCCAGA TAAGAGCCTT TAGAGACCCT CTACAGGATG ACACTGGATT ATATGTTACT 
GAGGAAGTCA CCTCCTCTGA AGATAAACGA AAGACTTATT CTTTGGCCTT TAAGCAGGAA 
TTAAAAGATG TGATCCTCTG TATCTCCCCA GTAATCACAT TCCGAGAACC CTTTCTTTTA 
ACTGAAAAGG GGATGAGATG CTCTACCCGA GATTATTTTG CAGAGCAGGT TTACTGGTCT 
CCTCTCCTCA ATAAAGAATT CAAAGAAATG GAGAACAGGA GGAAGAAACA GCTGCTCAGG 
GATCTCTCTG GACTTCAGGG CATGAATGGA AGTATTCAGG CCAAGTCTAT TCAAGTCTTA 
CCCTCACATG AGCTAGTGAG CACTAGAATT GCTGAGCATC TGGGCGATAG CCAGAGCTTG 
GGTAGAATGC TGGCCGATTA TCGAGCCAGA GGAGGAAGAA TTCAGCCCAA AAATTCAGAC 
CCTTTTGCTC ATTCAAAGGA TGCATCAAGT ACTTCAAGTG GCCAATCAGG AAGCAAAAAT 
GAGGGTGATG AAGAGAGAGG GCTTATTCTG AGTGATGCTG TGTGGTCAAC AAAGGTGGAC 
TGTCTGAATC CCATTAATCA CCAGAGACTT TGTGTGCTCT TCAGCAGCTC TTCTGCCCAG 
TCCAGCAATG CTCCTAGTGC CTGTGTCAGT CCTTGGATTG TAACAATGGA ATTTTATGGA 
AAGAATGATC TTACATTAGG AATATTTTTA GAGAGATACT GTTTCAGGCC TTCTTATCAG 
TGTCCAAGCA TGTTCTGTGA TACCCCCATG GTACATCATA TTCGGCGCTT TGTTCATGGC 
CAAGGCTGTG TGCAGATAAT CCTGAAGGAG TTGGATTCTC CAGTACCTGG ATATCAGCAT 
ACAATTCTTA CATATTCCTG GTGTAGAATC TGCAAACAGG TAACACCAGT TGTTGCTCTT 
TCCAATGAGT CCTGGTCTAT GTCATTTGCA AAATACCTTG AACTTAGGTT TTATGGGCAC 
CAGTATACTC GCAGAGCCAA CGCTGAGCCC TGTGGTCACT CCATCCATCA TGATTATCAC 
CAGTATTTCT CCTATAACCA GATGGTGGCG TCTTTCAGTT ATTCTCCCAT TCGGCTTCTT 
GAAGTATGTG TTCCACTCCC CAAAATATTC ATTAAGCGTC AGGCCCCATT AAAAGTGTCC 
CTTCTTCAGG ATCTGAAGGA CTTCTTTCAA AAAGTTTCAC AGGTATATGT TGCCATTGAT 
GAAAGACTTG CATCTTTGAA AACTGATACA TTTAGTAAAA CAAGAGAGGA AAAAATGGAA 
GATATTTTTG CACAGAAAGA GATGGAAGAA GGTGAGTTCA AGAACTGGAT TGAGAAGATG 
CAAGCAAGGC TCATGTCTTC CTCTGTAGAT ACCCCTCAGC AACTGCAGTC GGTCTTTGAG 
TCACTCATTG CCAAGAAACA AAGTCTCTGT GAAGTGCTGC AAGCTTGGAA TAACAGGTTG 
CAGGACCTTT TCCAACAGGA AAAGGGTAGA AAGAGACCTT CAGTTCCTCC AAGTCCTGGA 
AGACTGAGAC AAGGGGAAGA AAGCAAGATA AGTGCGATGG ATGCATCTCC ACGGAATATT 
TCTCCAGGAC TTCAGAATGG AGAAAAAGAG GATCGCTTCT TAACAACTTT GTCCAGCCAG 
AGCTCCACCA GTTCTACTCA TCTCCAATTG CCTACGCCAC CTGAAGTCAT GTCTGAACAG 
TCAGTGGGAG GGCCCCCTGA GCTAGATACA GCCAGCAGTT CCGAAGATGT GTTTGATGGG 
CATTTGCTGG GATCCACAGA CAGCCAAGTG AAGGAAAAGT CAACCATGAA AGCCATCTTT 
GCAAATTTGC TTCCAGGAAA TAGCTATAAT CCTATTCCAT TTCCTTTTGA TCCAGATAAA 
CACTACTTAA TGTATGAACA TGAACGAGTG CCCATTGCAG TCTGCGAGAA GGAACCCAGC 
TCCATCATTG CTTTTGCTCT CAGTTGTAAA GAATACCGAA ATGCCTTAGA GGAATTGTCT 
AAAGCGACTC AGTGGAACAG TGCCGAAGAA GGGCTTCCAA CAAATAGTAC TTCAGATAGC 
AGACCAAAGA GTAGCAGCCC TATCAGATTA CCTGAAATGA GTGGAGGACA GACAAATCGT 
ACAACAGAAA CAGAACCACA ACCAACCAAA AAGGCTTCTG GAATGTTGTC CTTCTTCAGA 
GGGACAGCAG GGAAAAGCCC CGATCTCTCT TCCCAGAAGA GAGAGACCTT ACGTGGAGCA 
GATAGTGCTT ACTACCAGGT TGGGCAGACG GGCAAGGAGG GGACCGAGAA TCAAGGCGTT 
GAGCCTCAAG ATGAAGTAGA TGGAGGAGAT ACACAAAAGA AGCAACTCAT AAATCCTCAT 
GTGGAACTTC AATTTTCAGA TGCTAATGCC AAGTTTTACT GTCGGCTCTA CTATGCGGGA 
GAGTTTCATA AGATGCGTGA AGTGATTCTG GACAGCAGTG AAGAAGATTT CATTCGTTCC 
CTCTCCCACT CATCACCCTG GCAGGCCCGG GGAGGCAAAT CAGGAGCTGC CTTCTATGCA 
ACTGAGGATG ATAGATTTAT TTTGAAGCAA ATGCCTCGTC TGGAAGTCCA GTCCTTCCTC 
GACTTTGCAC CACATTACTT CAATTATATT ACAAATGCTG TTCAACAAAA GAGGCCCACG 
GCGTTGGCCA AAATTCTTGG AGTTTACAGA ATTGGTTATA AGAACTCTCA GAACAACACT 
GAGAAGAAGT TAGATCTCCT TGTCATGGAA AATCTTTTCT ACGGGAGAAA GATGGCACAG 
GTTTTTGATT TGAAGGGCTC TCTTAGGAAT CGGAATGTAA AAACTGACAC TGGAAAAGAG 
AGTTGTGATG TGGTCCTGCT AGATGAAAAT CTCCTAAAGA TGGTTCGAGA CAACCCTCTA 
TATATTCGTT CTCATTCCAA AGCTGTGCTG AGAACCTCGA TCCATAGTGA CTCCCATTTC 
CTTTCTAGCC ACCTCATTAT AGATTATTCT TTGCTGGTTG GGCGAGATGA TACTAGCAAT 
GAGCTAGTAG TTGGAATTAT AGATTATATT CGAACATTTA CATGGGACAA AAAGCTTGAG 
ATGGTTGTGA AATCAACAGG AATTTTAGGT GGACAAGGTA AAATGCCAAC AGTGGTGTCT 
CCGGAGTTGT ACAGGACTAG GTTTTGTGAG GCAATGGACA AGTATTTCCT AATGGTACCA 
GACCACTGGA CAGGCTTGGG TCTGAATTGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011509081.1
CDS	106..6438
Translation	MATDDKTSPTLDSANDLPRSPTSPSHLTHFKPLTPDQDEPPFKSAYSSFVNLFRFNKERAEGGQGEQQPLSGSWTSPQLPSRTQSVRSPTPYKKQLNEELQRRSSALADNSLQHPQENTDTRRKAEPTFGGHDPRTAVQLRSLSTVLKRLKEIMEGKSQDSDLKQYWMPDSQCKECYDCSEKFTTFRRRHHCRLCGQIFCSRCCNQEIPGKFMGYTGDLRACTYCRKIALSYAHSTDSNSIGEDLNALSDSACSVSVLDPSEPRTPVGSRKASRNIFLEDDLAWQSLIHPDSSNTPLSTRLVSVQEDAGKSPARNRSASITNLSLDRSGSPMVPSYETSVSPQANRTYVRTETTEDERKILLDSVQLKDLWKKICHHSSGMEFQDHRYWLRTHPNCIVGKELVNWLIRNGHIATRAQAIAIGQAMVDGRWLDCVSHHDQLFRDEYALYRPLQSTEFSETPSPDSDSVNSVEGHSEPSWFKDIKFDDSDTEQIAEEGDDNLANSASPSKRTSVSSFQSTVDSDSAASISLNVELDNVNFHIKKPSKYPHVPPHPADQKEYLISDTGGQQLSISDAFIKESLFNRRVEEKSKELPFTPLGWHHNNLELLREENGEKQAMERLLSANHNHMMALLQQLLHSDSLSSSWRDIIVSLVCQVVQTVRPDVKNQDDDMDIRQFVHIKKIPGGKKFDSVVVNGFVCTKNIAHKKMSSCIKNPKILLLKCSIEYLYREETKFTCIDPIVLQEREFLKNYVQRIVDVRPTLVLVEKTVSRIAQDMLLEHGITLVINVKSQVLERISRMTQGDLVMSMDQLLTKPHLGTCHKFYMQIFQLPNEQTKTLMFFEGCPQHLGCTIKLRGGSDYELARVKEILIFMICVAYHSQLEISFLMDEFAMPPTLMQNPSFHSLIEGRGHEGAVQEQYGGGSIPWDPDIPPESLPCDDSSLLELRIVFEKGEQENKNLPQAVASVKHQEHSTTACPAGLPCAFFAPVPESLLPLPVDDQQDALGSEQPETLQQTVVLQDPKSQIRAFRDPLQDDTGLYVTEEVTSSEDKRKTYSLAFKQELKDVILCISPVITFREPFLLTEKGMRCSTRDYFAEQVYWSPLLNKEFKEMENRRKKQLLRDLSGLQGMNGSIQAKSIQVLPSHELVSTRIAEHLGDSQSLGRMLADYRARGGRIQPKNSDPFAHSKDASSTSSGQSGSKNEGDEERGLILSDAVWSTKVDCLNPINHQRLCVLFSSSSAQSSNAPSACVSPWIVTMEFYGKNDLTLGIFLERYCFRPSYQCPSMFCDTPMVHHIRRFVHGQGCVQIILKELDSPVPGYQHTILTYSWCRICKQVTPVVALSNESWSMSFAKYLELRFYGHQYTRRANAEPCGHSIHHDYHQYFSYNQMVASFSYSPIRLLEVCVPLPKIFIKRQAPLKVSLLQDLKDFFQKVSQVYVAIDERLASLKTDTFSKTREEKMEDIFAQKEMEEGEFKNWIEKMQARLMSSSVDTPQQLQSVFESLIAKKQSLCEVLQAWNNRLQDLFQQEKGRKRPSVPPSPGRLRQGEESKISAMDASPRNISPGLQNGEKEDRFLTTLSSQSSTSSTHLQLPTPPEVMSEQSVGGPPELDTASSSEDVFDGHLLGSTDSQVKEKSTMKAIFANLLPGNSYNPIPFPFDPDKHYLMYEHERVPIAVCEKEPSSIIAFALSCKEYRNALEELSKATQWNSAEEGLPTNSTSDSRPKSSSPIRLPEMSGGQTNRTTETEPQPTKKASGMLSFFRGTAGKSPDLSSQKRETLRGADSAYYQVGQTGKEGTENQGVEPQDEVDGGDTQKKQLINPHVELQFSDANAKFYCRLYYAGEFHKMREVILDSSEEDFIRSLSHSSPWQARGGKSGAAFYATEDDRFILKQMPRLEVQSFLDFAPHYFNYITNAVQQKRPTALAKILGVYRIGYKNSQNNTEKKLDLLVMENLFYGRKMAQVFDLKGSLRNRNVKTDTGKESCDVVLLDENLLKMVRDNPLYIRSHSKAVLRTSIHSDSHFLSSHLIIDYSLLVGRDDTSNELVVGIIDYIRTFTWDKKLEMVVKSTGILGGQGKMPTVVSPELYRTRFCEAMDKYFLMVPDHWTGLGLNC

Target ORF information:

RefSeq Version	XM_011510779.2
Organism	Homo sapiens(human)
Definition	Homo sapiens phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011510779.2

ORF Insert Sequence:

ATGGCCACAG ATGATAAGAC GTCCCCAACA CTGGACTCTG CTAATGATTT GCCTCGATCT 
CCTACTAGTC CTTCTCATCT CACACACTTT AAACCTTTGA CTCCTGATCA AGATGAGCCC 
CCTTTTAAAT CAGCTTATAG TTCTTTTGTA AATCTCTTTC GTTTTAACAA AGAGAGAGCA 
GAAGGAGGCC AGGGAGAACA GCAGCCTTTG AGTGGAAGTT GGACCAGCCC TCAGCTCCCT 
TCGAGGACAC AGTCTGTTAG GTCACCCACA CCTTATAAAA AGCAGCTTAA TGAGGAACTC 
CAGCGGCGCT CTTCAGCATT AGCAGACAAC AGTTTGCAAC ATCCCCAGGA GAACACAGAC 
ACAAGAAGGA AAGCAGAACC TACCTTTGGA GGTCATGACC CTCGTACAGC TGTTCAGCTT 
CGAAGCCTCA GCACAGTATT AAAACGCCTC AAGGAAATCA TGGAGGGGAA AAGCCAGGAT 
AGTGACCTGA AACAATACTG GATGCCAGAT AGCCAATGTA AAGAGTGCTA TGACTGTAGT 
GAGAAATTTA CAACCTTTAG GCGCAGACAC CATTGCCGAC TATGTGGGCA GATTTTCTGC 
AGTCGTTGCT GTAATCAAGA AATCCCTGGA AAATTTATGG GCTATACAGG AGACCTCCGA 
GCTTGCACAT ATTGTAGAAA AATAGCCTTA AGTTATGCTC ATTCCACAGA CAGTAATTCT 
ATTGGGGAAG ACTTGAATGC TCTTTCAGAT TCTGCTTGCT CTGTGTCTGT GCTTGATCCA 
AGTGAACCCC GAACACCTGT TGGGAGTAGG AAAGCCAGCC GTAACATATT TTTAGAGGAT 
GATTTGGCCT GGCAAAGTTT GATTCATCCA GATTCCTCAA ATACTCCTCT TTCAACAAGA 
CTTGTATCTG TGCAAGAGGA TGCTGGGAAA TCTCCTGCTC GAAATAGATC AGCCAGCATT 
ACTAACCTGT CACTGGATAG ATCTGGTTCT CCTATGGTAC CTTCATATGA GACATCTGTC 
AGTCCCCAGG CTAACCGAAC ATATGTTAGG ACAGAGACCA CTGAGGATGA ACGCAAAATT 
CTTCTGGACA GTGTGCAGTT AAAAGACCTG TGGAAAAAAA TCTGCCATCA CAGCAGTGGA 
ATGGAGTTTC AGGATCACCG CTACTGGTTG AGAACGCATC CCAACTGCAT TGTAGGAAAG 
GAATTAGTCA ACTGGCTAAT CCGAAATGGG CATATTGCCA CAAGGGCACA AGCTATAGCA 
ATTGGACAAG CAATGGTTGA TGGACGTTGG CTGGATTGTG TTAGTCATCA CGACCAGCTT 
TTCAGAGATG AGTATGCGCT GTATAGACCA CTGCAGAGTA CAGAATTTTC TGAGACGCCT 
TCTCCCGACA GTGACTCAGT GAACTCCGTG GAAGGACACT CTGAGCCATC CTGGTTTAAA 
GACATAAAGT TTGATGACAG TGACACAGAA CAGATAGCTG AAGAAGGTGA CGATAATTTG 
GCTAATTCTG CCAGTCCTAG CAAGCGCACA TCAGTCAGCA GTTTCCAGTC CACAGTGGAC 
AGTGACTCAG CCGCTTCTAT CAGCCTGAAC GTGGAGCTGG ACAACGTGAA CTTCCATATC 
AAGAAGCCCT CCAAGTACCC ACATGTGCCC CCTCACCCTG CTGACCAAAA AGAGTATTTG 
ATTTCTGACA CTGGAGGACA ACAGCTCTCA ATAAGTGACG CTTTCATCAA AGAATCCTTA 
TTTAATCGCC GAGTAGAGGA AAAATCCAAA GAGCTGCCTT TCACACCTTT GGGCTGGCAT 
CATAACAACC TGGAGCTCCT GAGGGAGGAG AATGGGGAGA AACAAGCCAT GGAGAGGTTG 
CTTTCAGCTA ATCATAACCA CATGATGGCA CTACTCCAGC AGTTGCTCCA TAGTGACTCA 
CTGTCATCAT CTTGGAGGGA CATCATCGTG TCATTGGTCT GCCAGGTTGT TCAGACAGTC 
CGACCTGATG TCAAGAACCA GGATGATGAC ATGGATATCC GTCAGTTTGT CCACATCAAA 
AAAATCCCAG GTGGAAAGAA GTTTGATTCT GTGGTTGTCA ATGGCTTTGT TTGTACCAAG 
AACATTGCAC ATAAAAAGAT GAGTTCTTGT ATTAAAAACC CCAAAATTCT TCTGTTGAAG 
TGTTCCATTG AGTATCTCTA CAGAGAAGAA ACTAAGTTTA CTTGCATTGA TCCTATTGTG 
CTTCAGGAAA GGGAATTCTT GAAGAATTAT GTCCAGCGAA TAGTTGATGT TCGACCCACC 
TTGGTTCTTG TTGAGAAAAC AGTGTCTCGG ATTGCCCAGG ACATGTTATT GGAACATGGC 
ATTACTTTGG TCATTAATGT AAAGTCACAA GTTTTGGAAC GAATCAGTCG AATGACCCAA 
GGTGATTTAG TGATGTCAAT GGACCAGCTG CTTACGAAAC CACACCTGGG CACTTGTCAC 
AAATTTTATA TGCAGATATT TCAGTTGCCT AATGAACAAA CCAAGACACT GATGTTTTTT 
GAAGGTTGTC CACAGCACCT AGGCTGTACA ATCAAGCTAA GAGGAGGCTC TGATTATGAG 
CTGGCTCGAG TTAAGGAGAT CCTAATATTT ATGATCTGTG TTGCTTATCA TTCTCAACTA 
GAAATATCCT TTCTCATGGA TGAATTTGCT ATGCCTCCCA CATTAATGCA AAACCCTTCA 
TTCCATTCCC TGATTGAGGG ACGAGGGCAT GAGGGGGCTG TCCAAGAGCA GTACGGTGGA 
GGTTCCATCC CCTGGGATCC TGACATCCCT CCTGAGTCTC TGCCCTGTGA TGATAGCAGT 
TTGCTGGAAT TGAGGATTGT GTTTGAGAAG GGTGAGCAGG AAAATAAAAA TCTTCCGCAG 
GCTGTTGCCT CTGTGAAGCA TCAAGAACAT AGCACAACAG CTTGCCCGGC GGGTCTCCCT 
TGTGCTTTCT TTGCACCTGT ACCGGAATCA TTGTTGCCAC TCCCTGTGGA TGACCAACAA 
GATGCTTTAG GCAGCGAGCA GCCAGAGACT TTGCAGCAAA CAGTTGTGCT GCAGGATCCC 
AAAAGCCAGA TAAGAGCCTT TAGAGACCCT CTACAGGATG ACACTGGATT ATATGTTACT 
GAGGAAGTCA CCTCCTCTGA AGATAAACGA AAGACTTATT CTTTGGCCTT TAAGCAGGAA 
TTAAAAGATG TGATCCTCTG TATCTCCCCA GTAATCACAT TCCGAGAACC CTTTCTTTTA 
ACTGAAAAGG GGATGAGATG CTCTACCCGA GATTATTTTG CAGAGCAGGT TTACTGGTCT 
CCTCTCCTCA ATAAAGAATT CAAAGAAATG GAGAACAGGA GGAAGAAACA GCTGCTCAGG 
GATCTCTCTG GACTTCAGGG CATGAATGGA AGTATTCAGG CCAAGTCTAT TCAAGTCTTA 
CCCTCACATG AGCTAGTGAG CACTAGAATT GCTGAGCATC TGGGCGATAG CCAGAGCTTG 
GGTAGAATGC TGGCCGATTA TCGAGCCAGA GGAGGAAGAA TTCAGCCCAA AAATTCAGAC 
CCTTTTGCTC ATTCAAAGGA TGCATCAAGT ACTTCAAGTG GCCAATCAGG AAGCAAAAAT 
GAGGGTGATG AAGAGAGAGG GCTTATTCTG AGTGATGCTG TGTGGTCAAC AAAGGTGGAC 
TGTCTGAATC CCATTAATCA CCAGAGACTT TGTGTGCTCT TCAGCAGCTC TTCTGCCCAG 
TCCAGCAATG CTCCTAGTGC CTGTGTCAGT CCTTGGATTG TAACAATGGA ATTTTATGGA 
AAGAATGATC TTACATTAGG AATATTTTTA GAGAGATACT GTTTCAGGCC TTCTTATCAG 
TGTCCAAGCA TGTTCTGTGA TACCCCCATG GTACATCATA TTCGGCGCTT TGTTCATGGC 
CAAGGCTGTG TGCAGATAAT CCTGAAGGAG TTGGATTCTC CAGTACCTGG ATATCAGCAT 
ACAATTCTTA CATATTCCTG GTGTAGAATC TGCAAACAGG TAACACCAGT TGTTGCTCTT 
TCCAATGAGT CCTGGTCTAT GTCATTTGCA AAATACCTTG AACTTAGGTT TTATGGGCAC 
CAGTATACTC GCAGAGCCAA CGCTGAGCCC TGTGGTCACT CCATCCATCA TGATTATCAC 
CAGTATTTCT CCTATAACCA GATGGTGGCG TCTTTCAGTT ATTCTCCCAT TCGGCTTCTT 
GAAGTATGTG TTCCACTCCC CAAAATATTC ATTAAGCGTC AGGCCCCATT AAAAGTGTCC 
CTTCTTCAGG ATCTGAAGGA CTTCTTTCAA AAAGTTTCAC AGGTATATGT TGCCATTGAT 
GAAAGACTTG CATCTTTGAA AACTGATACA TTTAGTAAAA CAAGAGAGGA AAAAATGGAA 
GATATTTTTG CACAGAAAGA GATGGAAGAA GGTGAGTTCA AGAACTGGAT TGAGAAGATG 
CAAGCAAGGC TCATGTCTTC CTCTGTAGAT ACCCCTCAGC AACTGCAGTC GGTCTTTGAG 
TCACTCATTG CCAAGAAACA AAGTCTCTGT GAAGTGCTGC AAGCTTGGAA TAACAGGTTG 
CAGGACCTTT TCCAACAGGA AAAGGGTAGA AAGAGACCTT CAGTTCCTCC AAGTCCTGGA 
AGACTGAGAC AAGGGGAAGA AAGCAAGATA AGTGCGATGG ATGCATCTCC ACGGAATATT 
TCTCCAGGAC TTCAGAATGG AGAAAAAGAG GATCGCTTCT TAACAACTTT GTCCAGCCAG 
AGCTCCACCA GTTCTACTCA TCTCCAATTG CCTACGCCAC CTGAAGTCAT GTCTGAACAG 
TCAGTGGGAG GGCCCCCTGA GCTAGATACA GCCAGCAGTT CCGAAGATGT GTTTGATGGG 
CATTTGCTGG GATCCACAGA CAGCCAAGTG AAGGAAAAGT CAACCATGAA AGCCATCTTT 
GCAAATTTGC TTCCAGGAAA TAGCTATAAT CCTATTCCAT TTCCTTTTGA TCCAGATAAA 
CACTACTTAA TGTATGAACA TGAACGAGTG CCCATTGCAG TCTGCGAGAA GGAACCCAGC 
TCCATCATTG CTTTTGCTCT CAGTTGTAAA GAATACCGAA ATGCCTTAGA GGAATTGTCT 
AAAGCGACTC AGTGGAACAG TGCCGAAGAA GGGCTTCCAA CAAATAGTAC TTCAGATAGC 
AGACCAAAGA GTAGCAGCCC TATCAGATTA CCTGAAATGA GTGGAGGACA GACAAATCGT 
ACAACAGAAA CAGAACCACA ACCAACCAAA AAGGCTTCTG GAATGTTGTC CTTCTTCAGA 
GGGACAGCAG GGAAAAGCCC CGATCTCTCT TCCCAGAAGA GAGAGACCTT ACGTGGAGCA 
GATAGTGCTT ACTACCAGGT TGGGCAGACG GGCAAGGAGG GGACCGAGAA TCAAGGCGTT 
GAGCCTCAAG ATGAAGTAGA TGGAGGAGAT ACACAAAAGA AGCAACTCAT AAATCCTCAT 
GTGGAACTTC AATTTTCAGA TGCTAATGCC AAGTTTTACT GTCGGCTCTA CTATGCGGGA 
GAGTTTCATA AGATGCGTGA AGTGATTCTG GACAGCAGTG AAGAAGATTT CATTCGTTCC 
CTCTCCCACT CATCACCCTG GCAGGCCCGG GGAGGCAAAT CAGGAGCTGC CTTCTATGCA 
ACTGAGGATG ATAGATTTAT TTTGAAGCAA ATGCCTCGTC TGGAAGTCCA GTCCTTCCTC 
GACTTTGCAC CACATTACTT CAATTATATT ACAAATGCTG TTCAACAAAA GAGGCCCACG 
GCGTTGGCCA AAATTCTTGG AGTTTACAGA ATTGGTTATA AGAACTCTCA GAACAACACT 
GAGAAGAAGT TAGATCTCCT TGTCATGGAA AATCTTTTCT ACGGGAGAAA GATGGCACAG 
GTTTTTGATT TGAAGGGCTC TCTTAGGAAT CGGAATGTAA AAACTGACAC TGGAAAAGAG 
AGTTGTGATG TGGTCCTGCT AGATGAAAAT CTCCTAAAGA TGGTTCGAGA CAACCCTCTA 
TATATTCGTT CTCATTCCAA AGCTGTGCTG AGAACCTCGA TCCATAGTGA CTCCCATTTC 
CTTTCTAGCC ACCTCATTAT AGATTATTCT TTGCTGGTTG GGCGAGATGA TACTAGCAAT 
GAGCTAGTAG TTGGAATTAT AGATTATATT CGAACATTTA CATGGGACAA AAAGCTTGAG 
ATGGTTGTGA AATCAACAGG AATTTTAGGT GGACAAGGTA AAATGCCAAC AGTGGTGTCT 
CCGGAGTTGT ACAGGACTAG GTTTTGTGAG GCAATGGACA AGTATTTCCT AATGGTACCA 
GACCACTGGA CAGGCTTGGG TCTGAATTGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PIKFYVE ( XM_011510780.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011509082.1 Homo sapiens phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X3, mRNA.

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CloneID	OHu77662
Clone ID Related Accession (Same CDS sequence)	XM_011510780.2
Accession Version	XM_011510780.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6330bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	1-phosphatidylinositol 3-phosphate 5-kinase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011510780.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG ATGATAAGAC GTCCCCAACA CTGGACTCTG CTAATGATTT GCCTCGATCT 
CCTACTAGTC CTTCTCATCT CACACACTTT AAACCTTTGA CTCCTGATCA AGATGAGCCC 
CCTTTTAAAT CAGCTTATAG TTCTTTTGTA AATCTCTTTC GTTTTAACAA AGAGAGAGCA 
GAAGGAGGCC AGGGAGAACA GCAGCCTTTG AGTGGAAGTT GGACCAGCCC TCAGCTCCCT 
TCGAGGACAC AGTCTGTTAG GTCACCCACA CCTTATAAAA AGCAGCTTAA TGAGGAACTC 
CAGCGGCGCT CTTCAGCATT AGACAACAGT TTGCAACATC CCCAGGAGAA CACAGACACA 
AGAAGGAAAG CAGAACCTAC CTTTGGAGGT CATGACCCTC GTACAGCTGT TCAGCTTCGA 
AGCCTCAGCA CAGTATTAAA ACGCCTCAAG GAAATCATGG AGGGGAAAAG CCAGGATAGT 
GACCTGAAAC AATACTGGAT GCCAGATAGC CAATGTAAAG AGTGCTATGA CTGTAGTGAG 
AAATTTACAA CCTTTAGGCG CAGACACCAT TGCCGACTAT GTGGGCAGAT TTTCTGCAGT 
CGTTGCTGTA ATCAAGAAAT CCCTGGAAAA TTTATGGGCT ATACAGGAGA CCTCCGAGCT 
TGCACATATT GTAGAAAAAT AGCCTTAAGT TATGCTCATT CCACAGACAG TAATTCTATT 
GGGGAAGACT TGAATGCTCT TTCAGATTCT GCTTGCTCTG TGTCTGTGCT TGATCCAAGT 
GAACCCCGAA CACCTGTTGG GAGTAGGAAA GCCAGCCGTA ACATATTTTT AGAGGATGAT 
TTGGCCTGGC AAAGTTTGAT TCATCCAGAT TCCTCAAATA CTCCTCTTTC AACAAGACTT 
GTATCTGTGC AAGAGGATGC TGGGAAATCT CCTGCTCGAA ATAGATCAGC CAGCATTACT 
AACCTGTCAC TGGATAGATC TGGTTCTCCT ATGGTACCTT CATATGAGAC ATCTGTCAGT 
CCCCAGGCTA ACCGAACATA TGTTAGGACA GAGACCACTG AGGATGAACG CAAAATTCTT 
CTGGACAGTG TGCAGTTAAA AGACCTGTGG AAAAAAATCT GCCATCACAG CAGTGGAATG 
GAGTTTCAGG ATCACCGCTA CTGGTTGAGA ACGCATCCCA ACTGCATTGT AGGAAAGGAA 
TTAGTCAACT GGCTAATCCG AAATGGGCAT ATTGCCACAA GGGCACAAGC TATAGCAATT 
GGACAAGCAA TGGTTGATGG ACGTTGGCTG GATTGTGTTA GTCATCACGA CCAGCTTTTC 
AGAGATGAGT ATGCGCTGTA TAGACCACTG CAGAGTACAG AATTTTCTGA GACGCCTTCT 
CCCGACAGTG ACTCAGTGAA CTCCGTGGAA GGACACTCTG AGCCATCCTG GTTTAAAGAC 
ATAAAGTTTG ATGACAGTGA CACAGAACAG ATAGCTGAAG AAGGTGACGA TAATTTGGCT 
AATTCTGCCA GTCCTAGCAA GCGCACATCA GTCAGCAGTT TCCAGTCCAC AGTGGACAGT 
GACTCAGCCG CTTCTATCAG CCTGAACGTG GAGCTGGACA ACGTGAACTT CCATATCAAG 
AAGCCCTCCA AGTACCCACA TGTGCCCCCT CACCCTGCTG ACCAAAAAGA GTATTTGATT 
TCTGACACTG GAGGACAACA GCTCTCAATA AGTGACGCTT TCATCAAAGA ATCCTTATTT 
AATCGCCGAG TAGAGGAAAA ATCCAAAGAG CTGCCTTTCA CACCTTTGGG CTGGCATCAT 
AACAACCTGG AGCTCCTGAG GGAGGAGAAT GGGGAGAAAC AAGCCATGGA GAGGTTGCTT 
TCAGCTAATC ATAACCACAT GATGGCACTA CTCCAGCAGT TGCTCCATAG TGACTCACTG 
TCATCATCTT GGAGGGACAT CATCGTGTCA TTGGTCTGCC AGGTTGTTCA GACAGTCCGA 
CCTGATGTCA AGAACCAGGA TGATGACATG GATATCCGTC AGTTTGTCCA CATCAAAAAA 
ATCCCAGGTG GAAAGAAGTT TGATTCTGTG GTTGTCAATG GCTTTGTTTG TACCAAGAAC 
ATTGCACATA AAAAGATGAG TTCTTGTATT AAAAACCCCA AAATTCTTCT GTTGAAGTGT 
TCCATTGAGT ATCTCTACAG AGAAGAAACT AAGTTTACTT GCATTGATCC TATTGTGCTT 
CAGGAAAGGG AATTCTTGAA GAATTATGTC CAGCGAATAG TTGATGTTCG ACCCACCTTG 
GTTCTTGTTG AGAAAACAGT GTCTCGGATT GCCCAGGACA TGTTATTGGA ACATGGCATT 
ACTTTGGTCA TTAATGTAAA GTCACAAGTT TTGGAACGAA TCAGTCGAAT GACCCAAGGT 
GATTTAGTGA TGTCAATGGA CCAGCTGCTT ACGAAACCAC ACCTGGGCAC TTGTCACAAA 
TTTTATATGC AGATATTTCA GTTGCCTAAT GAACAAACCA AGACACTGAT GTTTTTTGAA 
GGTTGTCCAC AGCACCTAGG CTGTACAATC AAGCTAAGAG GAGGCTCTGA TTATGAGCTG 
GCTCGAGTTA AGGAGATCCT AATATTTATG ATCTGTGTTG CTTATCATTC TCAACTAGAA 
ATATCCTTTC TCATGGATGA ATTTGCTATG CCTCCCACAT TAATGCAAAA CCCTTCATTC 
CATTCCCTGA TTGAGGGACG AGGGCATGAG GGGGCTGTCC AAGAGCAGTA CGGTGGAGGT 
TCCATCCCCT GGGATCCTGA CATCCCTCCT GAGTCTCTGC CCTGTGATGA TAGCAGTTTG 
CTGGAATTGA GGATTGTGTT TGAGAAGGGT GAGCAGGAAA ATAAAAATCT TCCGCAGGCT 
GTTGCCTCTG TGAAGCATCA AGAACATAGC ACAACAGCTT GCCCGGCGGG TCTCCCTTGT 
GCTTTCTTTG CACCTGTACC GGAATCATTG TTGCCACTCC CTGTGGATGA CCAACAAGAT 
GCTTTAGGCA GCGAGCAGCC AGAGACTTTG CAGCAAACAG TTGTGCTGCA GGATCCCAAA 
AGCCAGATAA GAGCCTTTAG AGACCCTCTA CAGGATGACA CTGGATTATA TGTTACTGAG 
GAAGTCACCT CCTCTGAAGA TAAACGAAAG ACTTATTCTT TGGCCTTTAA GCAGGAATTA 
AAAGATGTGA TCCTCTGTAT CTCCCCAGTA ATCACATTCC GAGAACCCTT TCTTTTAACT 
GAAAAGGGGA TGAGATGCTC TACCCGAGAT TATTTTGCAG AGCAGGTTTA CTGGTCTCCT 
CTCCTCAATA AAGAATTCAA AGAAATGGAG AACAGGAGGA AGAAACAGCT GCTCAGGGAT 
CTCTCTGGAC TTCAGGGCAT GAATGGAAGT ATTCAGGCCA AGTCTATTCA AGTCTTACCC 
TCACATGAGC TAGTGAGCAC TAGAATTGCT GAGCATCTGG GCGATAGCCA GAGCTTGGGT 
AGAATGCTGG CCGATTATCG AGCCAGAGGA GGAAGAATTC AGCCCAAAAA TTCAGACCCT 
TTTGCTCATT CAAAGGATGC ATCAAGTACT TCAAGTGGCC AATCAGGAAG CAAAAATGAG 
GGTGATGAAG AGAGAGGGCT TATTCTGAGT GATGCTGTGT GGTCAACAAA GGTGGACTGT 
CTGAATCCCA TTAATCACCA GAGACTTTGT GTGCTCTTCA GCAGCTCTTC TGCCCAGTCC 
AGCAATGCTC CTAGTGCCTG TGTCAGTCCT TGGATTGTAA CAATGGAATT TTATGGAAAG 
AATGATCTTA CATTAGGAAT ATTTTTAGAG AGATACTGTT TCAGGCCTTC TTATCAGTGT 
CCAAGCATGT TCTGTGATAC CCCCATGGTA CATCATATTC GGCGCTTTGT TCATGGCCAA 
GGCTGTGTGC AGATAATCCT GAAGGAGTTG GATTCTCCAG TACCTGGATA TCAGCATACA 
ATTCTTACAT ATTCCTGGTG TAGAATCTGC AAACAGGTAA CACCAGTTGT TGCTCTTTCC 
AATGAGTCCT GGTCTATGTC ATTTGCAAAA TACCTTGAAC TTAGGTTTTA TGGGCACCAG 
TATACTCGCA GAGCCAACGC TGAGCCCTGT GGTCACTCCA TCCATCATGA TTATCACCAG 
TATTTCTCCT ATAACCAGAT GGTGGCGTCT TTCAGTTATT CTCCCATTCG GCTTCTTGAA 
GTATGTGTTC CACTCCCCAA AATATTCATT AAGCGTCAGG CCCCATTAAA AGTGTCCCTT 
CTTCAGGATC TGAAGGACTT CTTTCAAAAA GTTTCACAGG TATATGTTGC CATTGATGAA 
AGACTTGCAT CTTTGAAAAC TGATACATTT AGTAAAACAA GAGAGGAAAA AATGGAAGAT 
ATTTTTGCAC AGAAAGAGAT GGAAGAAGGT GAGTTCAAGA ACTGGATTGA GAAGATGCAA 
GCAAGGCTCA TGTCTTCCTC TGTAGATACC CCTCAGCAAC TGCAGTCGGT CTTTGAGTCA 
CTCATTGCCA AGAAACAAAG TCTCTGTGAA GTGCTGCAAG CTTGGAATAA CAGGTTGCAG 
GACCTTTTCC AACAGGAAAA GGGTAGAAAG AGACCTTCAG TTCCTCCAAG TCCTGGAAGA 
CTGAGACAAG GGGAAGAAAG CAAGATAAGT GCGATGGATG CATCTCCACG GAATATTTCT 
CCAGGACTTC AGAATGGAGA AAAAGAGGAT CGCTTCTTAA CAACTTTGTC CAGCCAGAGC 
TCCACCAGTT CTACTCATCT CCAATTGCCT ACGCCACCTG AAGTCATGTC TGAACAGTCA 
GTGGGAGGGC CCCCTGAGCT AGATACAGCC AGCAGTTCCG AAGATGTGTT TGATGGGCAT 
TTGCTGGGAT CCACAGACAG CCAAGTGAAG GAAAAGTCAA CCATGAAAGC CATCTTTGCA 
AATTTGCTTC CAGGAAATAG CTATAATCCT ATTCCATTTC CTTTTGATCC AGATAAACAC 
TACTTAATGT ATGAACATGA ACGAGTGCCC ATTGCAGTCT GCGAGAAGGA ACCCAGCTCC 
ATCATTGCTT TTGCTCTCAG TTGTAAAGAA TACCGAAATG CCTTAGAGGA ATTGTCTAAA 
GCGACTCAGT GGAACAGTGC CGAAGAAGGG CTTCCAACAA ATAGTACTTC AGATAGCAGA 
CCAAAGAGTA GCAGCCCTAT CAGATTACCT GAAATGAGTG GAGGACAGAC AAATCGTACA 
ACAGAAACAG AACCACAACC AACCAAAAAG GCTTCTGGAA TGTTGTCCTT CTTCAGAGGG 
ACAGCAGGGA AAAGCCCCGA TCTCTCTTCC CAGAAGAGAG AGACCTTACG TGGAGCAGAT 
AGTGCTTACT ACCAGGTTGG GCAGACGGGC AAGGAGGGGA CCGAGAATCA AGGCGTTGAG 
CCTCAAGATG AAGTAGATGG AGGAGATACA CAAAAGAAGC AACTCATAAA TCCTCATGTG 
GAACTTCAAT TTTCAGATGC TAATGCCAAG TTTTACTGTC GGCTCTACTA TGCGGGAGAG 
TTTCATAAGA TGCGTGAAGT GATTCTGGAC AGCAGTGAAG AAGATTTCAT TCGTTCCCTC 
TCCCACTCAT CACCCTGGCA GGCCCGGGGA GGCAAATCAG GAGCTGCCTT CTATGCAACT 
GAGGATGATA GATTTATTTT GAAGCAAATG CCTCGTCTGG AAGTCCAGTC CTTCCTCGAC 
TTTGCACCAC ATTACTTCAA TTATATTACA AATGCTGTTC AACAAAAGAG GCCCACGGCG 
TTGGCCAAAA TTCTTGGAGT TTACAGAATT GGTTATAAGA ACTCTCAGAA CAACACTGAG 
AAGAAGTTAG ATCTCCTTGT CATGGAAAAT CTTTTCTACG GGAGAAAGAT GGCACAGGTT 
TTTGATTTGA AGGGCTCTCT TAGGAATCGG AATGTAAAAA CTGACACTGG AAAAGAGAGT 
TGTGATGTGG TCCTGCTAGA TGAAAATCTC CTAAAGATGG TTCGAGACAA CCCTCTATAT 
ATTCGTTCTC ATTCCAAAGC TGTGCTGAGA ACCTCGATCC ATAGTGACTC CCATTTCCTT 
TCTAGCCACC TCATTATAGA TTATTCTTTG CTGGTTGGGC GAGATGATAC TAGCAATGAG 
CTAGTAGTTG GAATTATAGA TTATATTCGA ACATTTACAT GGGACAAAAA GCTTGAGATG 
GTTGTGAAAT CAACAGGAAT TTTAGGTGGA CAAGGTAAAA TGCCAACAGT GGTGTCTCCG 
GAGTTGTACA GGACTAGGTT TTGTGAGGCA ATGGACAAGT ATTTCCTAAT GGTACCAGAC 
CACTGGACAG GCTTGGGTCT GAATTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011509082.1
CDS	194..6523
Translation	MATDDKTSPTLDSANDLPRSPTSPSHLTHFKPLTPDQDEPPFKSAYSSFVNLFRFNKERAEGGQGEQQPLSGSWTSPQLPSRTQSVRSPTPYKKQLNEELQRRSSALDNSLQHPQENTDTRRKAEPTFGGHDPRTAVQLRSLSTVLKRLKEIMEGKSQDSDLKQYWMPDSQCKECYDCSEKFTTFRRRHHCRLCGQIFCSRCCNQEIPGKFMGYTGDLRACTYCRKIALSYAHSTDSNSIGEDLNALSDSACSVSVLDPSEPRTPVGSRKASRNIFLEDDLAWQSLIHPDSSNTPLSTRLVSVQEDAGKSPARNRSASITNLSLDRSGSPMVPSYETSVSPQANRTYVRTETTEDERKILLDSVQLKDLWKKICHHSSGMEFQDHRYWLRTHPNCIVGKELVNWLIRNGHIATRAQAIAIGQAMVDGRWLDCVSHHDQLFRDEYALYRPLQSTEFSETPSPDSDSVNSVEGHSEPSWFKDIKFDDSDTEQIAEEGDDNLANSASPSKRTSVSSFQSTVDSDSAASISLNVELDNVNFHIKKPSKYPHVPPHPADQKEYLISDTGGQQLSISDAFIKESLFNRRVEEKSKELPFTPLGWHHNNLELLREENGEKQAMERLLSANHNHMMALLQQLLHSDSLSSSWRDIIVSLVCQVVQTVRPDVKNQDDDMDIRQFVHIKKIPGGKKFDSVVVNGFVCTKNIAHKKMSSCIKNPKILLLKCSIEYLYREETKFTCIDPIVLQEREFLKNYVQRIVDVRPTLVLVEKTVSRIAQDMLLEHGITLVINVKSQVLERISRMTQGDLVMSMDQLLTKPHLGTCHKFYMQIFQLPNEQTKTLMFFEGCPQHLGCTIKLRGGSDYELARVKEILIFMICVAYHSQLEISFLMDEFAMPPTLMQNPSFHSLIEGRGHEGAVQEQYGGGSIPWDPDIPPESLPCDDSSLLELRIVFEKGEQENKNLPQAVASVKHQEHSTTACPAGLPCAFFAPVPESLLPLPVDDQQDALGSEQPETLQQTVVLQDPKSQIRAFRDPLQDDTGLYVTEEVTSSEDKRKTYSLAFKQELKDVILCISPVITFREPFLLTEKGMRCSTRDYFAEQVYWSPLLNKEFKEMENRRKKQLLRDLSGLQGMNGSIQAKSIQVLPSHELVSTRIAEHLGDSQSLGRMLADYRARGGRIQPKNSDPFAHSKDASSTSSGQSGSKNEGDEERGLILSDAVWSTKVDCLNPINHQRLCVLFSSSSAQSSNAPSACVSPWIVTMEFYGKNDLTLGIFLERYCFRPSYQCPSMFCDTPMVHHIRRFVHGQGCVQIILKELDSPVPGYQHTILTYSWCRICKQVTPVVALSNESWSMSFAKYLELRFYGHQYTRRANAEPCGHSIHHDYHQYFSYNQMVASFSYSPIRLLEVCVPLPKIFIKRQAPLKVSLLQDLKDFFQKVSQVYVAIDERLASLKTDTFSKTREEKMEDIFAQKEMEEGEFKNWIEKMQARLMSSSVDTPQQLQSVFESLIAKKQSLCEVLQAWNNRLQDLFQQEKGRKRPSVPPSPGRLRQGEESKISAMDASPRNISPGLQNGEKEDRFLTTLSSQSSTSSTHLQLPTPPEVMSEQSVGGPPELDTASSSEDVFDGHLLGSTDSQVKEKSTMKAIFANLLPGNSYNPIPFPFDPDKHYLMYEHERVPIAVCEKEPSSIIAFALSCKEYRNALEELSKATQWNSAEEGLPTNSTSDSRPKSSSPIRLPEMSGGQTNRTTETEPQPTKKASGMLSFFRGTAGKSPDLSSQKRETLRGADSAYYQVGQTGKEGTENQGVEPQDEVDGGDTQKKQLINPHVELQFSDANAKFYCRLYYAGEFHKMREVILDSSEEDFIRSLSHSSPWQARGGKSGAAFYATEDDRFILKQMPRLEVQSFLDFAPHYFNYITNAVQQKRPTALAKILGVYRIGYKNSQNNTEKKLDLLVMENLFYGRKMAQVFDLKGSLRNRNVKTDTGKESCDVVLLDENLLKMVRDNPLYIRSHSKAVLRTSIHSDSHFLSSHLIIDYSLLVGRDDTSNELVVGIIDYIRTFTWDKKLEMVVKSTGILGGQGKMPTVVSPELYRTRFCEAMDKYFLMVPDHWTGLGLNC

Target ORF information:

RefSeq Version	XM_011510780.2
Organism	Homo sapiens(human)
Definition	Homo sapiens phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011510780.2

ORF Insert Sequence:

ATGGCCACAG ATGATAAGAC GTCCCCAACA CTGGACTCTG CTAATGATTT GCCTCGATCT 
CCTACTAGTC CTTCTCATCT CACACACTTT AAACCTTTGA CTCCTGATCA AGATGAGCCC 
CCTTTTAAAT CAGCTTATAG TTCTTTTGTA AATCTCTTTC GTTTTAACAA AGAGAGAGCA 
GAAGGAGGCC AGGGAGAACA GCAGCCTTTG AGTGGAAGTT GGACCAGCCC TCAGCTCCCT 
TCGAGGACAC AGTCTGTTAG GTCACCCACA CCTTATAAAA AGCAGCTTAA TGAGGAACTC 
CAGCGGCGCT CTTCAGCATT AGACAACAGT TTGCAACATC CCCAGGAGAA CACAGACACA 
AGAAGGAAAG CAGAACCTAC CTTTGGAGGT CATGACCCTC GTACAGCTGT TCAGCTTCGA 
AGCCTCAGCA CAGTATTAAA ACGCCTCAAG GAAATCATGG AGGGGAAAAG CCAGGATAGT 
GACCTGAAAC AATACTGGAT GCCAGATAGC CAATGTAAAG AGTGCTATGA CTGTAGTGAG 
AAATTTACAA CCTTTAGGCG CAGACACCAT TGCCGACTAT GTGGGCAGAT TTTCTGCAGT 
CGTTGCTGTA ATCAAGAAAT CCCTGGAAAA TTTATGGGCT ATACAGGAGA CCTCCGAGCT 
TGCACATATT GTAGAAAAAT AGCCTTAAGT TATGCTCATT CCACAGACAG TAATTCTATT 
GGGGAAGACT TGAATGCTCT TTCAGATTCT GCTTGCTCTG TGTCTGTGCT TGATCCAAGT 
GAACCCCGAA CACCTGTTGG GAGTAGGAAA GCCAGCCGTA ACATATTTTT AGAGGATGAT 
TTGGCCTGGC AAAGTTTGAT TCATCCAGAT TCCTCAAATA CTCCTCTTTC AACAAGACTT 
GTATCTGTGC AAGAGGATGC TGGGAAATCT CCTGCTCGAA ATAGATCAGC CAGCATTACT 
AACCTGTCAC TGGATAGATC TGGTTCTCCT ATGGTACCTT CATATGAGAC ATCTGTCAGT 
CCCCAGGCTA ACCGAACATA TGTTAGGACA GAGACCACTG AGGATGAACG CAAAATTCTT 
CTGGACAGTG TGCAGTTAAA AGACCTGTGG AAAAAAATCT GCCATCACAG CAGTGGAATG 
GAGTTTCAGG ATCACCGCTA CTGGTTGAGA ACGCATCCCA ACTGCATTGT AGGAAAGGAA 
TTAGTCAACT GGCTAATCCG AAATGGGCAT ATTGCCACAA GGGCACAAGC TATAGCAATT 
GGACAAGCAA TGGTTGATGG ACGTTGGCTG GATTGTGTTA GTCATCACGA CCAGCTTTTC 
AGAGATGAGT ATGCGCTGTA TAGACCACTG CAGAGTACAG AATTTTCTGA GACGCCTTCT 
CCCGACAGTG ACTCAGTGAA CTCCGTGGAA GGACACTCTG AGCCATCCTG GTTTAAAGAC 
ATAAAGTTTG ATGACAGTGA CACAGAACAG ATAGCTGAAG AAGGTGACGA TAATTTGGCT 
AATTCTGCCA GTCCTAGCAA GCGCACATCA GTCAGCAGTT TCCAGTCCAC AGTGGACAGT 
GACTCAGCCG CTTCTATCAG CCTGAACGTG GAGCTGGACA ACGTGAACTT CCATATCAAG 
AAGCCCTCCA AGTACCCACA TGTGCCCCCT CACCCTGCTG ACCAAAAAGA GTATTTGATT 
TCTGACACTG GAGGACAACA GCTCTCAATA AGTGACGCTT TCATCAAAGA ATCCTTATTT 
AATCGCCGAG TAGAGGAAAA ATCCAAAGAG CTGCCTTTCA CACCTTTGGG CTGGCATCAT 
AACAACCTGG AGCTCCTGAG GGAGGAGAAT GGGGAGAAAC AAGCCATGGA GAGGTTGCTT 
TCAGCTAATC ATAACCACAT GATGGCACTA CTCCAGCAGT TGCTCCATAG TGACTCACTG 
TCATCATCTT GGAGGGACAT CATCGTGTCA TTGGTCTGCC AGGTTGTTCA GACAGTCCGA 
CCTGATGTCA AGAACCAGGA TGATGACATG GATATCCGTC AGTTTGTCCA CATCAAAAAA 
ATCCCAGGTG GAAAGAAGTT TGATTCTGTG GTTGTCAATG GCTTTGTTTG TACCAAGAAC 
ATTGCACATA AAAAGATGAG TTCTTGTATT AAAAACCCCA AAATTCTTCT GTTGAAGTGT 
TCCATTGAGT ATCTCTACAG AGAAGAAACT AAGTTTACTT GCATTGATCC TATTGTGCTT 
CAGGAAAGGG AATTCTTGAA GAATTATGTC CAGCGAATAG TTGATGTTCG ACCCACCTTG 
GTTCTTGTTG AGAAAACAGT GTCTCGGATT GCCCAGGACA TGTTATTGGA ACATGGCATT 
ACTTTGGTCA TTAATGTAAA GTCACAAGTT TTGGAACGAA TCAGTCGAAT GACCCAAGGT 
GATTTAGTGA TGTCAATGGA CCAGCTGCTT ACGAAACCAC ACCTGGGCAC TTGTCACAAA 
TTTTATATGC AGATATTTCA GTTGCCTAAT GAACAAACCA AGACACTGAT GTTTTTTGAA 
GGTTGTCCAC AGCACCTAGG CTGTACAATC AAGCTAAGAG GAGGCTCTGA TTATGAGCTG 
GCTCGAGTTA AGGAGATCCT AATATTTATG ATCTGTGTTG CTTATCATTC TCAACTAGAA 
ATATCCTTTC TCATGGATGA ATTTGCTATG CCTCCCACAT TAATGCAAAA CCCTTCATTC 
CATTCCCTGA TTGAGGGACG AGGGCATGAG GGGGCTGTCC AAGAGCAGTA CGGTGGAGGT 
TCCATCCCCT GGGATCCTGA CATCCCTCCT GAGTCTCTGC CCTGTGATGA TAGCAGTTTG 
CTGGAATTGA GGATTGTGTT TGAGAAGGGT GAGCAGGAAA ATAAAAATCT TCCGCAGGCT 
GTTGCCTCTG TGAAGCATCA AGAACATAGC ACAACAGCTT GCCCGGCGGG TCTCCCTTGT 
GCTTTCTTTG CACCTGTACC GGAATCATTG TTGCCACTCC CTGTGGATGA CCAACAAGAT 
GCTTTAGGCA GCGAGCAGCC AGAGACTTTG CAGCAAACAG TTGTGCTGCA GGATCCCAAA 
AGCCAGATAA GAGCCTTTAG AGACCCTCTA CAGGATGACA CTGGATTATA TGTTACTGAG 
GAAGTCACCT CCTCTGAAGA TAAACGAAAG ACTTATTCTT TGGCCTTTAA GCAGGAATTA 
AAAGATGTGA TCCTCTGTAT CTCCCCAGTA ATCACATTCC GAGAACCCTT TCTTTTAACT 
GAAAAGGGGA TGAGATGCTC TACCCGAGAT TATTTTGCAG AGCAGGTTTA CTGGTCTCCT 
CTCCTCAATA AAGAATTCAA AGAAATGGAG AACAGGAGGA AGAAACAGCT GCTCAGGGAT 
CTCTCTGGAC TTCAGGGCAT GAATGGAAGT ATTCAGGCCA AGTCTATTCA AGTCTTACCC 
TCACATGAGC TAGTGAGCAC TAGAATTGCT GAGCATCTGG GCGATAGCCA GAGCTTGGGT 
AGAATGCTGG CCGATTATCG AGCCAGAGGA GGAAGAATTC AGCCCAAAAA TTCAGACCCT 
TTTGCTCATT CAAAGGATGC ATCAAGTACT TCAAGTGGCC AATCAGGAAG CAAAAATGAG 
GGTGATGAAG AGAGAGGGCT TATTCTGAGT GATGCTGTGT GGTCAACAAA GGTGGACTGT 
CTGAATCCCA TTAATCACCA GAGACTTTGT GTGCTCTTCA GCAGCTCTTC TGCCCAGTCC 
AGCAATGCTC CTAGTGCCTG TGTCAGTCCT TGGATTGTAA CAATGGAATT TTATGGAAAG 
AATGATCTTA CATTAGGAAT ATTTTTAGAG AGATACTGTT TCAGGCCTTC TTATCAGTGT 
CCAAGCATGT TCTGTGATAC CCCCATGGTA CATCATATTC GGCGCTTTGT TCATGGCCAA 
GGCTGTGTGC AGATAATCCT GAAGGAGTTG GATTCTCCAG TACCTGGATA TCAGCATACA 
ATTCTTACAT ATTCCTGGTG TAGAATCTGC AAACAGGTAA CACCAGTTGT TGCTCTTTCC 
AATGAGTCCT GGTCTATGTC ATTTGCAAAA TACCTTGAAC TTAGGTTTTA TGGGCACCAG 
TATACTCGCA GAGCCAACGC TGAGCCCTGT GGTCACTCCA TCCATCATGA TTATCACCAG 
TATTTCTCCT ATAACCAGAT GGTGGCGTCT TTCAGTTATT CTCCCATTCG GCTTCTTGAA 
GTATGTGTTC CACTCCCCAA AATATTCATT AAGCGTCAGG CCCCATTAAA AGTGTCCCTT 
CTTCAGGATC TGAAGGACTT CTTTCAAAAA GTTTCACAGG TATATGTTGC CATTGATGAA 
AGACTTGCAT CTTTGAAAAC TGATACATTT AGTAAAACAA GAGAGGAAAA AATGGAAGAT 
ATTTTTGCAC AGAAAGAGAT GGAAGAAGGT GAGTTCAAGA ACTGGATTGA GAAGATGCAA 
GCAAGGCTCA TGTCTTCCTC TGTAGATACC CCTCAGCAAC TGCAGTCGGT CTTTGAGTCA 
CTCATTGCCA AGAAACAAAG TCTCTGTGAA GTGCTGCAAG CTTGGAATAA CAGGTTGCAG 
GACCTTTTCC AACAGGAAAA GGGTAGAAAG AGACCTTCAG TTCCTCCAAG TCCTGGAAGA 
CTGAGACAAG GGGAAGAAAG CAAGATAAGT GCGATGGATG CATCTCCACG GAATATTTCT 
CCAGGACTTC AGAATGGAGA AAAAGAGGAT CGCTTCTTAA CAACTTTGTC CAGCCAGAGC 
TCCACCAGTT CTACTCATCT CCAATTGCCT ACGCCACCTG AAGTCATGTC TGAACAGTCA 
GTGGGAGGGC CCCCTGAGCT AGATACAGCC AGCAGTTCCG AAGATGTGTT TGATGGGCAT 
TTGCTGGGAT CCACAGACAG CCAAGTGAAG GAAAAGTCAA CCATGAAAGC CATCTTTGCA 
AATTTGCTTC CAGGAAATAG CTATAATCCT ATTCCATTTC CTTTTGATCC AGATAAACAC 
TACTTAATGT ATGAACATGA ACGAGTGCCC ATTGCAGTCT GCGAGAAGGA ACCCAGCTCC 
ATCATTGCTT TTGCTCTCAG TTGTAAAGAA TACCGAAATG CCTTAGAGGA ATTGTCTAAA 
GCGACTCAGT GGAACAGTGC CGAAGAAGGG CTTCCAACAA ATAGTACTTC AGATAGCAGA 
CCAAAGAGTA GCAGCCCTAT CAGATTACCT GAAATGAGTG GAGGACAGAC AAATCGTACA 
ACAGAAACAG AACCACAACC AACCAAAAAG GCTTCTGGAA TGTTGTCCTT CTTCAGAGGG 
ACAGCAGGGA AAAGCCCCGA TCTCTCTTCC CAGAAGAGAG AGACCTTACG TGGAGCAGAT 
AGTGCTTACT ACCAGGTTGG GCAGACGGGC AAGGAGGGGA CCGAGAATCA AGGCGTTGAG 
CCTCAAGATG AAGTAGATGG AGGAGATACA CAAAAGAAGC AACTCATAAA TCCTCATGTG 
GAACTTCAAT TTTCAGATGC TAATGCCAAG TTTTACTGTC GGCTCTACTA TGCGGGAGAG 
TTTCATAAGA TGCGTGAAGT GATTCTGGAC AGCAGTGAAG AAGATTTCAT TCGTTCCCTC 
TCCCACTCAT CACCCTGGCA GGCCCGGGGA GGCAAATCAG GAGCTGCCTT CTATGCAACT 
GAGGATGATA GATTTATTTT GAAGCAAATG CCTCGTCTGG AAGTCCAGTC CTTCCTCGAC 
TTTGCACCAC ATTACTTCAA TTATATTACA AATGCTGTTC AACAAAAGAG GCCCACGGCG 
TTGGCCAAAA TTCTTGGAGT TTACAGAATT GGTTATAAGA ACTCTCAGAA CAACACTGAG 
AAGAAGTTAG ATCTCCTTGT CATGGAAAAT CTTTTCTACG GGAGAAAGAT GGCACAGGTT 
TTTGATTTGA AGGGCTCTCT TAGGAATCGG AATGTAAAAA CTGACACTGG AAAAGAGAGT 
TGTGATGTGG TCCTGCTAGA TGAAAATCTC CTAAAGATGG TTCGAGACAA CCCTCTATAT 
ATTCGTTCTC ATTCCAAAGC TGTGCTGAGA ACCTCGATCC ATAGTGACTC CCATTTCCTT 
TCTAGCCACC TCATTATAGA TTATTCTTTG CTGGTTGGGC GAGATGATAC TAGCAATGAG 
CTAGTAGTTG GAATTATAGA TTATATTCGA ACATTTACAT GGGACAAAAA GCTTGAGATG 
GTTGTGAAAT CAACAGGAAT TTTAGGTGGA CAAGGTAAAA TGCCAACAGT GGTGTCTCCG 
GAGTTGTACA GGACTAGGTT TTGTGAGGCA ATGGACAAGT ATTTCCTAAT GGTACCAGAC 
CACTGGACAG GCTTGGGTCT GAATTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PIKFYVE ( XM_011510781.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011509083.1 Homo sapiens phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X4, mRNA.

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CloneID	OHu77663
Clone ID Related Accession (Same CDS sequence)	XM_011510781.2 , XM_011510781.3
Accession Version	XM_011510781.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6315bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	1-phosphatidylinositol 3-phosphate 5-kinase isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011510781.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG ATGATAAGAC GTCCCCAACA CTGGACTCTG CTAATGATTT GCCTCGATCT 
CCTACTAGTC CTTCTCATCT CACACACTTT AAACCTTTGA CTCCTGATCA AGATGAGCCC 
CCTTTTAAAT CAGCTTATAG TTCTTTTGTA AATCTCTTTC GTTTTAACAA AGAGAGAGCA 
GAAGGAGGCC AGGGAGAACA GCAGCCTTTG AGTGGAAGTT GGACCAGCCC TCAGCTCCCT 
TCGAGGACAC AGTCTGTTAG GTCACCCACA CCTTATAAAA AGCAGCTTAA TGAGGAACTC 
CAGCGGCGCT CTTCAGCATT AGCAGACAAC AGTTTGCAAC ATCCCCAGGA GAACACAGAC 
ACAAGAAGGA AAGCAGAACC TACCTTTGGA GGTCATGACC CTCGTACAGC TGTTCAGCTT 
CGAAGCCTCA GCACAGTATT AAAACGCCTC AAGGAAATCA TGGAGGGGAA AAGCCAGGAT 
AGTGACCTGA AACAATACTG GATGCCAGAT AGCCAATGTA AAGAGTGCTA TGACTGTAGT 
GAGAAATTTA CAACCTTTAG GCGCAGACAC CATTGCCGAC TATGTGGGCA GATTTTCTGC 
AGTCGTTGCT GTAATCAAGA AATCCCTGGA AAATTTATGG GCTATACAGG AGACCTCCGA 
GCTTGCACAT ATTGTAGAAA AATAGCCTTA AGTTATGCTC ATTCCACAGA CAGTAATTCT 
ATTGGGGAAG ACTTGAATGC TCTTTCAGAT TCTGCTTGCT CTGTGTCTGT GCTTGATCCA 
AGTGAACCCC GAACACCTGT TGGGAGTAGG AAAGCCAGCC GTAACATATT TTTAGAGGAT 
GATTTGGCCT GGCAAAGTTT GATTCATCCA GATTCCTCAA ATACTCCTCT TTCAACAAGA 
CTTGTATCTG TGCAAGAGGA TGCTGGGAAA TCTCCTGCTC GAAATAGATC AGCCAGCATT 
ACTAACCTGT CACTGGATAG ATCTGGTTCT CCTATGGTAC CTTCATATGA GACATCTGTC 
AGTCCCCAGG CTAACCGAAC ATATGTTAGG ACAGAGACCA CTGAGGATGA ACGCAAAATT 
CTTCTGGACA GTGTGCAGTT AAAAGACCTG TGGAAAAAAA TCTGCCATCA CAGCAGTGGA 
ATGGAGTTTC AGGATCACCG CTACTGGTTG AGAACGCATC CCAACTGCAT TGTAGGAAAG 
GAATTAGTCA ACTGGCTAAT CCGAAATGGG CATATTGCCA CAAGGGCACA AGCTATAGCA 
ATTGGACAAG CAATGGTTGA TGGACGTTGG CTGGATTGTG TTAGTCATCA CGACCAGCTT 
TTCAGAGATG AGTATGCGCT GTATAGACCA CTGCAGAGTA CAGAATTTTC TGAGACGCCT 
TCTCCCGACA GTGACTCAGT GAACTCCGTG GAAGGACACT CTGAGCCATC CTGGTTTAAA 
GACATAAAGT TTGATGACAG TGACACAGAA CAGATAGCTG AAGAAGATTC TGCCAGTCCT 
AGCAAGCGCA CATCAGTCAG CAGTTTCCAG TCCACAGTGG ACAGTGACTC AGCCGCTTCT 
ATCAGCCTGA ACGTGGAGCT GGACAACGTG AACTTCCATA TCAAGAAGCC CTCCAAGTAC 
CCACATGTGC CCCCTCACCC TGCTGACCAA AAAGAGTATT TGATTTCTGA CACTGGAGGA 
CAACAGCTCT CAATAAGTGA CGCTTTCATC AAAGAATCCT TATTTAATCG CCGAGTAGAG 
GAAAAATCCA AAGAGCTGCC TTTCACACCT TTGGGCTGGC ATCATAACAA CCTGGAGCTC 
CTGAGGGAGG AGAATGGGGA GAAACAAGCC ATGGAGAGGT TGCTTTCAGC TAATCATAAC 
CACATGATGG CACTACTCCA GCAGTTGCTC CATAGTGACT CACTGTCATC ATCTTGGAGG 
GACATCATCG TGTCATTGGT CTGCCAGGTT GTTCAGACAG TCCGACCTGA TGTCAAGAAC 
CAGGATGATG ACATGGATAT CCGTCAGTTT GTCCACATCA AAAAAATCCC AGGTGGAAAG 
AAGTTTGATT CTGTGGTTGT CAATGGCTTT GTTTGTACCA AGAACATTGC ACATAAAAAG 
ATGAGTTCTT GTATTAAAAA CCCCAAAATT CTTCTGTTGA AGTGTTCCAT TGAGTATCTC 
TACAGAGAAG AAACTAAGTT TACTTGCATT GATCCTATTG TGCTTCAGGA AAGGGAATTC 
TTGAAGAATT ATGTCCAGCG AATAGTTGAT GTTCGACCCA CCTTGGTTCT TGTTGAGAAA 
ACAGTGTCTC GGATTGCCCA GGACATGTTA TTGGAACATG GCATTACTTT GGTCATTAAT 
GTAAAGTCAC AAGTTTTGGA ACGAATCAGT CGAATGACCC AAGGTGATTT AGTGATGTCA 
ATGGACCAGC TGCTTACGAA ACCACACCTG GGCACTTGTC ACAAATTTTA TATGCAGATA 
TTTCAGTTGC CTAATGAACA AACCAAGACA CTGATGTTTT TTGAAGGTTG TCCACAGCAC 
CTAGGCTGTA CAATCAAGCT AAGAGGAGGC TCTGATTATG AGCTGGCTCG AGTTAAGGAG 
ATCCTAATAT TTATGATCTG TGTTGCTTAT CATTCTCAAC TAGAAATATC CTTTCTCATG 
GATGAATTTG CTATGCCTCC CACATTAATG CAAAACCCTT CATTCCATTC CCTGATTGAG 
GGACGAGGGC ATGAGGGGGC TGTCCAAGAG CAGTACGGTG GAGGTTCCAT CCCCTGGGAT 
CCTGACATCC CTCCTGAGTC TCTGCCCTGT GATGATAGCA GTTTGCTGGA ATTGAGGATT 
GTGTTTGAGA AGGGTGAGCA GGAAAATAAA AATCTTCCGC AGGCTGTTGC CTCTGTGAAG 
CATCAAGAAC ATAGCACAAC AGCTTGCCCG GCGGGTCTCC CTTGTGCTTT CTTTGCACCT 
GTACCGGAAT CATTGTTGCC ACTCCCTGTG GATGACCAAC AAGATGCTTT AGGCAGCGAG 
CAGCCAGAGA CTTTGCAGCA AACAGTTGTG CTGCAGGATC CCAAAAGCCA GATAAGAGCC 
TTTAGAGACC CTCTACAGGA TGACACTGGA TTATATGTTA CTGAGGAAGT CACCTCCTCT 
GAAGATAAAC GAAAGACTTA TTCTTTGGCC TTTAAGCAGG AATTAAAAGA TGTGATCCTC 
TGTATCTCCC CAGTAATCAC ATTCCGAGAA CCCTTTCTTT TAACTGAAAA GGGGATGAGA 
TGCTCTACCC GAGATTATTT TGCAGAGCAG GTTTACTGGT CTCCTCTCCT CAATAAAGAA 
TTCAAAGAAA TGGAGAACAG GAGGAAGAAA CAGCTGCTCA GGGATCTCTC TGGACTTCAG 
GGCATGAATG GAAGTATTCA GGCCAAGTCT ATTCAAGTCT TACCCTCACA TGAGCTAGTG 
AGCACTAGAA TTGCTGAGCA TCTGGGCGAT AGCCAGAGCT TGGGTAGAAT GCTGGCCGAT 
TATCGAGCCA GAGGAGGAAG AATTCAGCCC AAAAATTCAG ACCCTTTTGC TCATTCAAAG 
GATGCATCAA GTACTTCAAG TGGCCAATCA GGAAGCAAAA ATGAGGGTGA TGAAGAGAGA 
GGGCTTATTC TGAGTGATGC TGTGTGGTCA ACAAAGGTGG ACTGTCTGAA TCCCATTAAT 
CACCAGAGAC TTTGTGTGCT CTTCAGCAGC TCTTCTGCCC AGTCCAGCAA TGCTCCTAGT 
GCCTGTGTCA GTCCTTGGAT TGTAACAATG GAATTTTATG GAAAGAATGA TCTTACATTA 
GGAATATTTT TAGAGAGATA CTGTTTCAGG CCTTCTTATC AGTGTCCAAG CATGTTCTGT 
GATACCCCCA TGGTACATCA TATTCGGCGC TTTGTTCATG GCCAAGGCTG TGTGCAGATA 
ATCCTGAAGG AGTTGGATTC TCCAGTACCT GGATATCAGC ATACAATTCT TACATATTCC 
TGGTGTAGAA TCTGCAAACA GGTAACACCA GTTGTTGCTC TTTCCAATGA GTCCTGGTCT 
ATGTCATTTG CAAAATACCT TGAACTTAGG TTTTATGGGC ACCAGTATAC TCGCAGAGCC 
AACGCTGAGC CCTGTGGTCA CTCCATCCAT CATGATTATC ACCAGTATTT CTCCTATAAC 
CAGATGGTGG CGTCTTTCAG TTATTCTCCC ATTCGGCTTC TTGAAGTATG TGTTCCACTC 
CCCAAAATAT TCATTAAGCG TCAGGCCCCA TTAAAAGTGT CCCTTCTTCA GGATCTGAAG 
GACTTCTTTC AAAAAGTTTC ACAGGTATAT GTTGCCATTG ATGAAAGACT TGCATCTTTG 
AAAACTGATA CATTTAGTAA AACAAGAGAG GAAAAAATGG AAGATATTTT TGCACAGAAA 
GAGATGGAAG AAGGTGAGTT CAAGAACTGG ATTGAGAAGA TGCAAGCAAG GCTCATGTCT 
TCCTCTGTAG ATACCCCTCA GCAACTGCAG TCGGTCTTTG AGTCACTCAT TGCCAAGAAA 
CAAAGTCTCT GTGAAGTGCT GCAAGCTTGG AATAACAGGT TGCAGGACCT TTTCCAACAG 
GAAAAGGGTA GAAAGAGACC TTCAGTTCCT CCAAGTCCTG GAAGACTGAG ACAAGGGGAA 
GAAAGCAAGA TAAGTGCGAT GGATGCATCT CCACGGAATA TTTCTCCAGG ACTTCAGAAT 
GGAGAAAAAG AGGATCGCTT CTTAACAACT TTGTCCAGCC AGAGCTCCAC CAGTTCTACT 
CATCTCCAAT TGCCTACGCC ACCTGAAGTC ATGTCTGAAC AGTCAGTGGG AGGGCCCCCT 
GAGCTAGATA CAGCCAGCAG TTCCGAAGAT GTGTTTGATG GGCATTTGCT GGGATCCACA 
GACAGCCAAG TGAAGGAAAA GTCAACCATG AAAGCCATCT TTGCAAATTT GCTTCCAGGA 
AATAGCTATA ATCCTATTCC ATTTCCTTTT GATCCAGATA AACACTACTT AATGTATGAA 
CATGAACGAG TGCCCATTGC AGTCTGCGAG AAGGAACCCA GCTCCATCAT TGCTTTTGCT 
CTCAGTTGTA AAGAATACCG AAATGCCTTA GAGGAATTGT CTAAAGCGAC TCAGTGGAAC 
AGTGCCGAAG AAGGGCTTCC AACAAATAGT ACTTCAGATA GCAGACCAAA GAGTAGCAGC 
CCTATCAGAT TACCTGAAAT GAGTGGAGGA CAGACAAATC GTACAACAGA AACAGAACCA 
CAACCAACCA AAAAGGCTTC TGGAATGTTG TCCTTCTTCA GAGGGACAGC AGGGAAAAGC 
CCCGATCTCT CTTCCCAGAA GAGAGAGACC TTACGTGGAG CAGATAGTGC TTACTACCAG 
GTTGGGCAGA CGGGCAAGGA GGGGACCGAG AATCAAGGCG TTGAGCCTCA AGATGAAGTA 
GATGGAGGAG ATACACAAAA GAAGCAACTC ATAAATCCTC ATGTGGAACT TCAATTTTCA 
GATGCTAATG CCAAGTTTTA CTGTCGGCTC TACTATGCGG GAGAGTTTCA TAAGATGCGT 
GAAGTGATTC TGGACAGCAG TGAAGAAGAT TTCATTCGTT CCCTCTCCCA CTCATCACCC 
TGGCAGGCCC GGGGAGGCAA ATCAGGAGCT GCCTTCTATG CAACTGAGGA TGATAGATTT 
ATTTTGAAGC AAATGCCTCG TCTGGAAGTC CAGTCCTTCC TCGACTTTGC ACCACATTAC 
TTCAATTATA TTACAAATGC TGTTCAACAA AAGAGGCCCA CGGCGTTGGC CAAAATTCTT 
GGAGTTTACA GAATTGGTTA TAAGAACTCT CAGAACAACA CTGAGAAGAA GTTAGATCTC 
CTTGTCATGG AAAATCTTTT CTACGGGAGA AAGATGGCAC AGGTTTTTGA TTTGAAGGGC 
TCTCTTAGGA ATCGGAATGT AAAAACTGAC ACTGGAAAAG AGAGTTGTGA TGTGGTCCTG 
CTAGATGAAA ATCTCCTAAA GATGGTTCGA GACAACCCTC TATATATTCG TTCTCATTCC 
AAAGCTGTGC TGAGAACCTC GATCCATAGT GACTCCCATT TCCTTTCTAG CCACCTCATT 
ATAGATTATT CTTTGCTGGT TGGGCGAGAT GATACTAGCA ATGAGCTAGT AGTTGGAATT 
ATAGATTATA TTCGAACATT TACATGGGAC AAAAAGCTTG AGATGGTTGT GAAATCAACA 
GGAATTTTAG GTGGACAAGG TAAAATGCCA ACAGTGGTGT CTCCGGAGTT GTACAGGACT 
AGGTTTTGTG AGGCAATGGA CAAGTATTTC CTAATGGTAC CAGACCACTG GACAGGCTTG 
GGTCTGAATT GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011509083.1
CDS	132..6446
Translation	MATDDKTSPTLDSANDLPRSPTSPSHLTHFKPLTPDQDEPPFKSAYSSFVNLFRFNKERAEGGQGEQQPLSGSWTSPQLPSRTQSVRSPTPYKKQLNEELQRRSSALADNSLQHPQENTDTRRKAEPTFGGHDPRTAVQLRSLSTVLKRLKEIMEGKSQDSDLKQYWMPDSQCKECYDCSEKFTTFRRRHHCRLCGQIFCSRCCNQEIPGKFMGYTGDLRACTYCRKIALSYAHSTDSNSIGEDLNALSDSACSVSVLDPSEPRTPVGSRKASRNIFLEDDLAWQSLIHPDSSNTPLSTRLVSVQEDAGKSPARNRSASITNLSLDRSGSPMVPSYETSVSPQANRTYVRTETTEDERKILLDSVQLKDLWKKICHHSSGMEFQDHRYWLRTHPNCIVGKELVNWLIRNGHIATRAQAIAIGQAMVDGRWLDCVSHHDQLFRDEYALYRPLQSTEFSETPSPDSDSVNSVEGHSEPSWFKDIKFDDSDTEQIAEEDSASPSKRTSVSSFQSTVDSDSAASISLNVELDNVNFHIKKPSKYPHVPPHPADQKEYLISDTGGQQLSISDAFIKESLFNRRVEEKSKELPFTPLGWHHNNLELLREENGEKQAMERLLSANHNHMMALLQQLLHSDSLSSSWRDIIVSLVCQVVQTVRPDVKNQDDDMDIRQFVHIKKIPGGKKFDSVVVNGFVCTKNIAHKKMSSCIKNPKILLLKCSIEYLYREETKFTCIDPIVLQEREFLKNYVQRIVDVRPTLVLVEKTVSRIAQDMLLEHGITLVINVKSQVLERISRMTQGDLVMSMDQLLTKPHLGTCHKFYMQIFQLPNEQTKTLMFFEGCPQHLGCTIKLRGGSDYELARVKEILIFMICVAYHSQLEISFLMDEFAMPPTLMQNPSFHSLIEGRGHEGAVQEQYGGGSIPWDPDIPPESLPCDDSSLLELRIVFEKGEQENKNLPQAVASVKHQEHSTTACPAGLPCAFFAPVPESLLPLPVDDQQDALGSEQPETLQQTVVLQDPKSQIRAFRDPLQDDTGLYVTEEVTSSEDKRKTYSLAFKQELKDVILCISPVITFREPFLLTEKGMRCSTRDYFAEQVYWSPLLNKEFKEMENRRKKQLLRDLSGLQGMNGSIQAKSIQVLPSHELVSTRIAEHLGDSQSLGRMLADYRARGGRIQPKNSDPFAHSKDASSTSSGQSGSKNEGDEERGLILSDAVWSTKVDCLNPINHQRLCVLFSSSSAQSSNAPSACVSPWIVTMEFYGKNDLTLGIFLERYCFRPSYQCPSMFCDTPMVHHIRRFVHGQGCVQIILKELDSPVPGYQHTILTYSWCRICKQVTPVVALSNESWSMSFAKYLELRFYGHQYTRRANAEPCGHSIHHDYHQYFSYNQMVASFSYSPIRLLEVCVPLPKIFIKRQAPLKVSLLQDLKDFFQKVSQVYVAIDERLASLKTDTFSKTREEKMEDIFAQKEMEEGEFKNWIEKMQARLMSSSVDTPQQLQSVFESLIAKKQSLCEVLQAWNNRLQDLFQQEKGRKRPSVPPSPGRLRQGEESKISAMDASPRNISPGLQNGEKEDRFLTTLSSQSSTSSTHLQLPTPPEVMSEQSVGGPPELDTASSSEDVFDGHLLGSTDSQVKEKSTMKAIFANLLPGNSYNPIPFPFDPDKHYLMYEHERVPIAVCEKEPSSIIAFALSCKEYRNALEELSKATQWNSAEEGLPTNSTSDSRPKSSSPIRLPEMSGGQTNRTTETEPQPTKKASGMLSFFRGTAGKSPDLSSQKRETLRGADSAYYQVGQTGKEGTENQGVEPQDEVDGGDTQKKQLINPHVELQFSDANAKFYCRLYYAGEFHKMREVILDSSEEDFIRSLSHSSPWQARGGKSGAAFYATEDDRFILKQMPRLEVQSFLDFAPHYFNYITNAVQQKRPTALAKILGVYRIGYKNSQNNTEKKLDLLVMENLFYGRKMAQVFDLKGSLRNRNVKTDTGKESCDVVLLDENLLKMVRDNPLYIRSHSKAVLRTSIHSDSHFLSSHLIIDYSLLVGRDDTSNELVVGIIDYIRTFTWDKKLEMVVKSTGILGGQGKMPTVVSPELYRTRFCEAMDKYFLMVPDHWTGLGLNC

Target ORF information:

RefSeq Version	XM_011510781.2
Organism	Homo sapiens(human)
Definition	Homo sapiens phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011510781.2

ORF Insert Sequence:

ATGGCCACAG ATGATAAGAC GTCCCCAACA CTGGACTCTG CTAATGATTT GCCTCGATCT 
CCTACTAGTC CTTCTCATCT CACACACTTT AAACCTTTGA CTCCTGATCA AGATGAGCCC 
CCTTTTAAAT CAGCTTATAG TTCTTTTGTA AATCTCTTTC GTTTTAACAA AGAGAGAGCA 
GAAGGAGGCC AGGGAGAACA GCAGCCTTTG AGTGGAAGTT GGACCAGCCC TCAGCTCCCT 
TCGAGGACAC AGTCTGTTAG GTCACCCACA CCTTATAAAA AGCAGCTTAA TGAGGAACTC 
CAGCGGCGCT CTTCAGCATT AGCAGACAAC AGTTTGCAAC ATCCCCAGGA GAACACAGAC 
ACAAGAAGGA AAGCAGAACC TACCTTTGGA GGTCATGACC CTCGTACAGC TGTTCAGCTT 
CGAAGCCTCA GCACAGTATT AAAACGCCTC AAGGAAATCA TGGAGGGGAA AAGCCAGGAT 
AGTGACCTGA AACAATACTG GATGCCAGAT AGCCAATGTA AAGAGTGCTA TGACTGTAGT 
GAGAAATTTA CAACCTTTAG GCGCAGACAC CATTGCCGAC TATGTGGGCA GATTTTCTGC 
AGTCGTTGCT GTAATCAAGA AATCCCTGGA AAATTTATGG GCTATACAGG AGACCTCCGA 
GCTTGCACAT ATTGTAGAAA AATAGCCTTA AGTTATGCTC ATTCCACAGA CAGTAATTCT 
ATTGGGGAAG ACTTGAATGC TCTTTCAGAT TCTGCTTGCT CTGTGTCTGT GCTTGATCCA 
AGTGAACCCC GAACACCTGT TGGGAGTAGG AAAGCCAGCC GTAACATATT TTTAGAGGAT 
GATTTGGCCT GGCAAAGTTT GATTCATCCA GATTCCTCAA ATACTCCTCT TTCAACAAGA 
CTTGTATCTG TGCAAGAGGA TGCTGGGAAA TCTCCTGCTC GAAATAGATC AGCCAGCATT 
ACTAACCTGT CACTGGATAG ATCTGGTTCT CCTATGGTAC CTTCATATGA GACATCTGTC 
AGTCCCCAGG CTAACCGAAC ATATGTTAGG ACAGAGACCA CTGAGGATGA ACGCAAAATT 
CTTCTGGACA GTGTGCAGTT AAAAGACCTG TGGAAAAAAA TCTGCCATCA CAGCAGTGGA 
ATGGAGTTTC AGGATCACCG CTACTGGTTG AGAACGCATC CCAACTGCAT TGTAGGAAAG 
GAATTAGTCA ACTGGCTAAT CCGAAATGGG CATATTGCCA CAAGGGCACA AGCTATAGCA 
ATTGGACAAG CAATGGTTGA TGGACGTTGG CTGGATTGTG TTAGTCATCA CGACCAGCTT 
TTCAGAGATG AGTATGCGCT GTATAGACCA CTGCAGAGTA CAGAATTTTC TGAGACGCCT 
TCTCCCGACA GTGACTCAGT GAACTCCGTG GAAGGACACT CTGAGCCATC CTGGTTTAAA 
GACATAAAGT TTGATGACAG TGACACAGAA CAGATAGCTG AAGAAGATTC TGCCAGTCCT 
AGCAAGCGCA CATCAGTCAG CAGTTTCCAG TCCACAGTGG ACAGTGACTC AGCCGCTTCT 
ATCAGCCTGA ACGTGGAGCT GGACAACGTG AACTTCCATA TCAAGAAGCC CTCCAAGTAC 
CCACATGTGC CCCCTCACCC TGCTGACCAA AAAGAGTATT TGATTTCTGA CACTGGAGGA 
CAACAGCTCT CAATAAGTGA CGCTTTCATC AAAGAATCCT TATTTAATCG CCGAGTAGAG 
GAAAAATCCA AAGAGCTGCC TTTCACACCT TTGGGCTGGC ATCATAACAA CCTGGAGCTC 
CTGAGGGAGG AGAATGGGGA GAAACAAGCC ATGGAGAGGT TGCTTTCAGC TAATCATAAC 
CACATGATGG CACTACTCCA GCAGTTGCTC CATAGTGACT CACTGTCATC ATCTTGGAGG 
GACATCATCG TGTCATTGGT CTGCCAGGTT GTTCAGACAG TCCGACCTGA TGTCAAGAAC 
CAGGATGATG ACATGGATAT CCGTCAGTTT GTCCACATCA AAAAAATCCC AGGTGGAAAG 
AAGTTTGATT CTGTGGTTGT CAATGGCTTT GTTTGTACCA AGAACATTGC ACATAAAAAG 
ATGAGTTCTT GTATTAAAAA CCCCAAAATT CTTCTGTTGA AGTGTTCCAT TGAGTATCTC 
TACAGAGAAG AAACTAAGTT TACTTGCATT GATCCTATTG TGCTTCAGGA AAGGGAATTC 
TTGAAGAATT ATGTCCAGCG AATAGTTGAT GTTCGACCCA CCTTGGTTCT TGTTGAGAAA 
ACAGTGTCTC GGATTGCCCA GGACATGTTA TTGGAACATG GCATTACTTT GGTCATTAAT 
GTAAAGTCAC AAGTTTTGGA ACGAATCAGT CGAATGACCC AAGGTGATTT AGTGATGTCA 
ATGGACCAGC TGCTTACGAA ACCACACCTG GGCACTTGTC ACAAATTTTA TATGCAGATA 
TTTCAGTTGC CTAATGAACA AACCAAGACA CTGATGTTTT TTGAAGGTTG TCCACAGCAC 
CTAGGCTGTA CAATCAAGCT AAGAGGAGGC TCTGATTATG AGCTGGCTCG AGTTAAGGAG 
ATCCTAATAT TTATGATCTG TGTTGCTTAT CATTCTCAAC TAGAAATATC CTTTCTCATG 
GATGAATTTG CTATGCCTCC CACATTAATG CAAAACCCTT CATTCCATTC CCTGATTGAG 
GGACGAGGGC ATGAGGGGGC TGTCCAAGAG CAGTACGGTG GAGGTTCCAT CCCCTGGGAT 
CCTGACATCC CTCCTGAGTC TCTGCCCTGT GATGATAGCA GTTTGCTGGA ATTGAGGATT 
GTGTTTGAGA AGGGTGAGCA GGAAAATAAA AATCTTCCGC AGGCTGTTGC CTCTGTGAAG 
CATCAAGAAC ATAGCACAAC AGCTTGCCCG GCGGGTCTCC CTTGTGCTTT CTTTGCACCT 
GTACCGGAAT CATTGTTGCC ACTCCCTGTG GATGACCAAC AAGATGCTTT AGGCAGCGAG 
CAGCCAGAGA CTTTGCAGCA AACAGTTGTG CTGCAGGATC CCAAAAGCCA GATAAGAGCC 
TTTAGAGACC CTCTACAGGA TGACACTGGA TTATATGTTA CTGAGGAAGT CACCTCCTCT 
GAAGATAAAC GAAAGACTTA TTCTTTGGCC TTTAAGCAGG AATTAAAAGA TGTGATCCTC 
TGTATCTCCC CAGTAATCAC ATTCCGAGAA CCCTTTCTTT TAACTGAAAA GGGGATGAGA 
TGCTCTACCC GAGATTATTT TGCAGAGCAG GTTTACTGGT CTCCTCTCCT CAATAAAGAA 
TTCAAAGAAA TGGAGAACAG GAGGAAGAAA CAGCTGCTCA GGGATCTCTC TGGACTTCAG 
GGCATGAATG GAAGTATTCA GGCCAAGTCT ATTCAAGTCT TACCCTCACA TGAGCTAGTG 
AGCACTAGAA TTGCTGAGCA TCTGGGCGAT AGCCAGAGCT TGGGTAGAAT GCTGGCCGAT 
TATCGAGCCA GAGGAGGAAG AATTCAGCCC AAAAATTCAG ACCCTTTTGC TCATTCAAAG 
GATGCATCAA GTACTTCAAG TGGCCAATCA GGAAGCAAAA ATGAGGGTGA TGAAGAGAGA 
GGGCTTATTC TGAGTGATGC TGTGTGGTCA ACAAAGGTGG ACTGTCTGAA TCCCATTAAT 
CACCAGAGAC TTTGTGTGCT CTTCAGCAGC TCTTCTGCCC AGTCCAGCAA TGCTCCTAGT 
GCCTGTGTCA GTCCTTGGAT TGTAACAATG GAATTTTATG GAAAGAATGA TCTTACATTA 
GGAATATTTT TAGAGAGATA CTGTTTCAGG CCTTCTTATC AGTGTCCAAG CATGTTCTGT 
GATACCCCCA TGGTACATCA TATTCGGCGC TTTGTTCATG GCCAAGGCTG TGTGCAGATA 
ATCCTGAAGG AGTTGGATTC TCCAGTACCT GGATATCAGC ATACAATTCT TACATATTCC 
TGGTGTAGAA TCTGCAAACA GGTAACACCA GTTGTTGCTC TTTCCAATGA GTCCTGGTCT 
ATGTCATTTG CAAAATACCT TGAACTTAGG TTTTATGGGC ACCAGTATAC TCGCAGAGCC 
AACGCTGAGC CCTGTGGTCA CTCCATCCAT CATGATTATC ACCAGTATTT CTCCTATAAC 
CAGATGGTGG CGTCTTTCAG TTATTCTCCC ATTCGGCTTC TTGAAGTATG TGTTCCACTC 
CCCAAAATAT TCATTAAGCG TCAGGCCCCA TTAAAAGTGT CCCTTCTTCA GGATCTGAAG 
GACTTCTTTC AAAAAGTTTC ACAGGTATAT GTTGCCATTG ATGAAAGACT TGCATCTTTG 
AAAACTGATA CATTTAGTAA AACAAGAGAG GAAAAAATGG AAGATATTTT TGCACAGAAA 
GAGATGGAAG AAGGTGAGTT CAAGAACTGG ATTGAGAAGA TGCAAGCAAG GCTCATGTCT 
TCCTCTGTAG ATACCCCTCA GCAACTGCAG TCGGTCTTTG AGTCACTCAT TGCCAAGAAA 
CAAAGTCTCT GTGAAGTGCT GCAAGCTTGG AATAACAGGT TGCAGGACCT TTTCCAACAG 
GAAAAGGGTA GAAAGAGACC TTCAGTTCCT CCAAGTCCTG GAAGACTGAG ACAAGGGGAA 
GAAAGCAAGA TAAGTGCGAT GGATGCATCT CCACGGAATA TTTCTCCAGG ACTTCAGAAT 
GGAGAAAAAG AGGATCGCTT CTTAACAACT TTGTCCAGCC AGAGCTCCAC CAGTTCTACT 
CATCTCCAAT TGCCTACGCC ACCTGAAGTC ATGTCTGAAC AGTCAGTGGG AGGGCCCCCT 
GAGCTAGATA CAGCCAGCAG TTCCGAAGAT GTGTTTGATG GGCATTTGCT GGGATCCACA 
GACAGCCAAG TGAAGGAAAA GTCAACCATG AAAGCCATCT TTGCAAATTT GCTTCCAGGA 
AATAGCTATA ATCCTATTCC ATTTCCTTTT GATCCAGATA AACACTACTT AATGTATGAA 
CATGAACGAG TGCCCATTGC AGTCTGCGAG AAGGAACCCA GCTCCATCAT TGCTTTTGCT 
CTCAGTTGTA AAGAATACCG AAATGCCTTA GAGGAATTGT CTAAAGCGAC TCAGTGGAAC 
AGTGCCGAAG AAGGGCTTCC AACAAATAGT ACTTCAGATA GCAGACCAAA GAGTAGCAGC 
CCTATCAGAT TACCTGAAAT GAGTGGAGGA CAGACAAATC GTACAACAGA AACAGAACCA 
CAACCAACCA AAAAGGCTTC TGGAATGTTG TCCTTCTTCA GAGGGACAGC AGGGAAAAGC 
CCCGATCTCT CTTCCCAGAA GAGAGAGACC TTACGTGGAG CAGATAGTGC TTACTACCAG 
GTTGGGCAGA CGGGCAAGGA GGGGACCGAG AATCAAGGCG TTGAGCCTCA AGATGAAGTA 
GATGGAGGAG ATACACAAAA GAAGCAACTC ATAAATCCTC ATGTGGAACT TCAATTTTCA 
GATGCTAATG CCAAGTTTTA CTGTCGGCTC TACTATGCGG GAGAGTTTCA TAAGATGCGT 
GAAGTGATTC TGGACAGCAG TGAAGAAGAT TTCATTCGTT CCCTCTCCCA CTCATCACCC 
TGGCAGGCCC GGGGAGGCAA ATCAGGAGCT GCCTTCTATG CAACTGAGGA TGATAGATTT 
ATTTTGAAGC AAATGCCTCG TCTGGAAGTC CAGTCCTTCC TCGACTTTGC ACCACATTAC 
TTCAATTATA TTACAAATGC TGTTCAACAA AAGAGGCCCA CGGCGTTGGC CAAAATTCTT 
GGAGTTTACA GAATTGGTTA TAAGAACTCT CAGAACAACA CTGAGAAGAA GTTAGATCTC 
CTTGTCATGG AAAATCTTTT CTACGGGAGA AAGATGGCAC AGGTTTTTGA TTTGAAGGGC 
TCTCTTAGGA ATCGGAATGT AAAAACTGAC ACTGGAAAAG AGAGTTGTGA TGTGGTCCTG 
CTAGATGAAA ATCTCCTAAA GATGGTTCGA GACAACCCTC TATATATTCG TTCTCATTCC 
AAAGCTGTGC TGAGAACCTC GATCCATAGT GACTCCCATT TCCTTTCTAG CCACCTCATT 
ATAGATTATT CTTTGCTGGT TGGGCGAGAT GATACTAGCA ATGAGCTAGT AGTTGGAATT 
ATAGATTATA TTCGAACATT TACATGGGAC AAAAAGCTTG AGATGGTTGT GAAATCAACA 
GGAATTTTAG GTGGACAAGG TAAAATGCCA ACAGTGGTGT CTCCGGAGTT GTACAGGACT 
AGGTTTTGTG AGGCAATGGA CAAGTATTTC CTAATGGTAC CAGACCACTG GACAGGCTTG 
GGTCTGAATT GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PIKFYVE ( XM_011510781.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011509083.1 Homo sapiens phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X4, mRNA.

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CloneID	OHu77663
Clone ID Related Accession (Same CDS sequence)	XM_011510781.2 , XM_011510781.3
Accession Version	XM_011510781.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6315bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	1-phosphatidylinositol 3-phosphate 5-kinase isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011510781.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG ATGATAAGAC GTCCCCAACA CTGGACTCTG CTAATGATTT GCCTCGATCT 
CCTACTAGTC CTTCTCATCT CACACACTTT AAACCTTTGA CTCCTGATCA AGATGAGCCC 
CCTTTTAAAT CAGCTTATAG TTCTTTTGTA AATCTCTTTC GTTTTAACAA AGAGAGAGCA 
GAAGGAGGCC AGGGAGAACA GCAGCCTTTG AGTGGAAGTT GGACCAGCCC TCAGCTCCCT 
TCGAGGACAC AGTCTGTTAG GTCACCCACA CCTTATAAAA AGCAGCTTAA TGAGGAACTC 
CAGCGGCGCT CTTCAGCATT AGCAGACAAC AGTTTGCAAC ATCCCCAGGA GAACACAGAC 
ACAAGAAGGA AAGCAGAACC TACCTTTGGA GGTCATGACC CTCGTACAGC TGTTCAGCTT 
CGAAGCCTCA GCACAGTATT AAAACGCCTC AAGGAAATCA TGGAGGGGAA AAGCCAGGAT 
AGTGACCTGA AACAATACTG GATGCCAGAT AGCCAATGTA AAGAGTGCTA TGACTGTAGT 
GAGAAATTTA CAACCTTTAG GCGCAGACAC CATTGCCGAC TATGTGGGCA GATTTTCTGC 
AGTCGTTGCT GTAATCAAGA AATCCCTGGA AAATTTATGG GCTATACAGG AGACCTCCGA 
GCTTGCACAT ATTGTAGAAA AATAGCCTTA AGTTATGCTC ATTCCACAGA CAGTAATTCT 
ATTGGGGAAG ACTTGAATGC TCTTTCAGAT TCTGCTTGCT CTGTGTCTGT GCTTGATCCA 
AGTGAACCCC GAACACCTGT TGGGAGTAGG AAAGCCAGCC GTAACATATT TTTAGAGGAT 
GATTTGGCCT GGCAAAGTTT GATTCATCCA GATTCCTCAA ATACTCCTCT TTCAACAAGA 
CTTGTATCTG TGCAAGAGGA TGCTGGGAAA TCTCCTGCTC GAAATAGATC AGCCAGCATT 
ACTAACCTGT CACTGGATAG ATCTGGTTCT CCTATGGTAC CTTCATATGA GACATCTGTC 
AGTCCCCAGG CTAACCGAAC ATATGTTAGG ACAGAGACCA CTGAGGATGA ACGCAAAATT 
CTTCTGGACA GTGTGCAGTT AAAAGACCTG TGGAAAAAAA TCTGCCATCA CAGCAGTGGA 
ATGGAGTTTC AGGATCACCG CTACTGGTTG AGAACGCATC CCAACTGCAT TGTAGGAAAG 
GAATTAGTCA ACTGGCTAAT CCGAAATGGG CATATTGCCA CAAGGGCACA AGCTATAGCA 
ATTGGACAAG CAATGGTTGA TGGACGTTGG CTGGATTGTG TTAGTCATCA CGACCAGCTT 
TTCAGAGATG AGTATGCGCT GTATAGACCA CTGCAGAGTA CAGAATTTTC TGAGACGCCT 
TCTCCCGACA GTGACTCAGT GAACTCCGTG GAAGGACACT CTGAGCCATC CTGGTTTAAA 
GACATAAAGT TTGATGACAG TGACACAGAA CAGATAGCTG AAGAAGATTC TGCCAGTCCT 
AGCAAGCGCA CATCAGTCAG CAGTTTCCAG TCCACAGTGG ACAGTGACTC AGCCGCTTCT 
ATCAGCCTGA ACGTGGAGCT GGACAACGTG AACTTCCATA TCAAGAAGCC CTCCAAGTAC 
CCACATGTGC CCCCTCACCC TGCTGACCAA AAAGAGTATT TGATTTCTGA CACTGGAGGA 
CAACAGCTCT CAATAAGTGA CGCTTTCATC AAAGAATCCT TATTTAATCG CCGAGTAGAG 
GAAAAATCCA AAGAGCTGCC TTTCACACCT TTGGGCTGGC ATCATAACAA CCTGGAGCTC 
CTGAGGGAGG AGAATGGGGA GAAACAAGCC ATGGAGAGGT TGCTTTCAGC TAATCATAAC 
CACATGATGG CACTACTCCA GCAGTTGCTC CATAGTGACT CACTGTCATC ATCTTGGAGG 
GACATCATCG TGTCATTGGT CTGCCAGGTT GTTCAGACAG TCCGACCTGA TGTCAAGAAC 
CAGGATGATG ACATGGATAT CCGTCAGTTT GTCCACATCA AAAAAATCCC AGGTGGAAAG 
AAGTTTGATT CTGTGGTTGT CAATGGCTTT GTTTGTACCA AGAACATTGC ACATAAAAAG 
ATGAGTTCTT GTATTAAAAA CCCCAAAATT CTTCTGTTGA AGTGTTCCAT TGAGTATCTC 
TACAGAGAAG AAACTAAGTT TACTTGCATT GATCCTATTG TGCTTCAGGA AAGGGAATTC 
TTGAAGAATT ATGTCCAGCG AATAGTTGAT GTTCGACCCA CCTTGGTTCT TGTTGAGAAA 
ACAGTGTCTC GGATTGCCCA GGACATGTTA TTGGAACATG GCATTACTTT GGTCATTAAT 
GTAAAGTCAC AAGTTTTGGA ACGAATCAGT CGAATGACCC AAGGTGATTT AGTGATGTCA 
ATGGACCAGC TGCTTACGAA ACCACACCTG GGCACTTGTC ACAAATTTTA TATGCAGATA 
TTTCAGTTGC CTAATGAACA AACCAAGACA CTGATGTTTT TTGAAGGTTG TCCACAGCAC 
CTAGGCTGTA CAATCAAGCT AAGAGGAGGC TCTGATTATG AGCTGGCTCG AGTTAAGGAG 
ATCCTAATAT TTATGATCTG TGTTGCTTAT CATTCTCAAC TAGAAATATC CTTTCTCATG 
GATGAATTTG CTATGCCTCC CACATTAATG CAAAACCCTT CATTCCATTC CCTGATTGAG 
GGACGAGGGC ATGAGGGGGC TGTCCAAGAG CAGTACGGTG GAGGTTCCAT CCCCTGGGAT 
CCTGACATCC CTCCTGAGTC TCTGCCCTGT GATGATAGCA GTTTGCTGGA ATTGAGGATT 
GTGTTTGAGA AGGGTGAGCA GGAAAATAAA AATCTTCCGC AGGCTGTTGC CTCTGTGAAG 
CATCAAGAAC ATAGCACAAC AGCTTGCCCG GCGGGTCTCC CTTGTGCTTT CTTTGCACCT 
GTACCGGAAT CATTGTTGCC ACTCCCTGTG GATGACCAAC AAGATGCTTT AGGCAGCGAG 
CAGCCAGAGA CTTTGCAGCA AACAGTTGTG CTGCAGGATC CCAAAAGCCA GATAAGAGCC 
TTTAGAGACC CTCTACAGGA TGACACTGGA TTATATGTTA CTGAGGAAGT CACCTCCTCT 
GAAGATAAAC GAAAGACTTA TTCTTTGGCC TTTAAGCAGG AATTAAAAGA TGTGATCCTC 
TGTATCTCCC CAGTAATCAC ATTCCGAGAA CCCTTTCTTT TAACTGAAAA GGGGATGAGA 
TGCTCTACCC GAGATTATTT TGCAGAGCAG GTTTACTGGT CTCCTCTCCT CAATAAAGAA 
TTCAAAGAAA TGGAGAACAG GAGGAAGAAA CAGCTGCTCA GGGATCTCTC TGGACTTCAG 
GGCATGAATG GAAGTATTCA GGCCAAGTCT ATTCAAGTCT TACCCTCACA TGAGCTAGTG 
AGCACTAGAA TTGCTGAGCA TCTGGGCGAT AGCCAGAGCT TGGGTAGAAT GCTGGCCGAT 
TATCGAGCCA GAGGAGGAAG AATTCAGCCC AAAAATTCAG ACCCTTTTGC TCATTCAAAG 
GATGCATCAA GTACTTCAAG TGGCCAATCA GGAAGCAAAA ATGAGGGTGA TGAAGAGAGA 
GGGCTTATTC TGAGTGATGC TGTGTGGTCA ACAAAGGTGG ACTGTCTGAA TCCCATTAAT 
CACCAGAGAC TTTGTGTGCT CTTCAGCAGC TCTTCTGCCC AGTCCAGCAA TGCTCCTAGT 
GCCTGTGTCA GTCCTTGGAT TGTAACAATG GAATTTTATG GAAAGAATGA TCTTACATTA 
GGAATATTTT TAGAGAGATA CTGTTTCAGG CCTTCTTATC AGTGTCCAAG CATGTTCTGT 
GATACCCCCA TGGTACATCA TATTCGGCGC TTTGTTCATG GCCAAGGCTG TGTGCAGATA 
ATCCTGAAGG AGTTGGATTC TCCAGTACCT GGATATCAGC ATACAATTCT TACATATTCC 
TGGTGTAGAA TCTGCAAACA GGTAACACCA GTTGTTGCTC TTTCCAATGA GTCCTGGTCT 
ATGTCATTTG CAAAATACCT TGAACTTAGG TTTTATGGGC ACCAGTATAC TCGCAGAGCC 
AACGCTGAGC CCTGTGGTCA CTCCATCCAT CATGATTATC ACCAGTATTT CTCCTATAAC 
CAGATGGTGG CGTCTTTCAG TTATTCTCCC ATTCGGCTTC TTGAAGTATG TGTTCCACTC 
CCCAAAATAT TCATTAAGCG TCAGGCCCCA TTAAAAGTGT CCCTTCTTCA GGATCTGAAG 
GACTTCTTTC AAAAAGTTTC ACAGGTATAT GTTGCCATTG ATGAAAGACT TGCATCTTTG 
AAAACTGATA CATTTAGTAA AACAAGAGAG GAAAAAATGG AAGATATTTT TGCACAGAAA 
GAGATGGAAG AAGGTGAGTT CAAGAACTGG ATTGAGAAGA TGCAAGCAAG GCTCATGTCT 
TCCTCTGTAG ATACCCCTCA GCAACTGCAG TCGGTCTTTG AGTCACTCAT TGCCAAGAAA 
CAAAGTCTCT GTGAAGTGCT GCAAGCTTGG AATAACAGGT TGCAGGACCT TTTCCAACAG 
GAAAAGGGTA GAAAGAGACC TTCAGTTCCT CCAAGTCCTG GAAGACTGAG ACAAGGGGAA 
GAAAGCAAGA TAAGTGCGAT GGATGCATCT CCACGGAATA TTTCTCCAGG ACTTCAGAAT 
GGAGAAAAAG AGGATCGCTT CTTAACAACT TTGTCCAGCC AGAGCTCCAC CAGTTCTACT 
CATCTCCAAT TGCCTACGCC ACCTGAAGTC ATGTCTGAAC AGTCAGTGGG AGGGCCCCCT 
GAGCTAGATA CAGCCAGCAG TTCCGAAGAT GTGTTTGATG GGCATTTGCT GGGATCCACA 
GACAGCCAAG TGAAGGAAAA GTCAACCATG AAAGCCATCT TTGCAAATTT GCTTCCAGGA 
AATAGCTATA ATCCTATTCC ATTTCCTTTT GATCCAGATA AACACTACTT AATGTATGAA 
CATGAACGAG TGCCCATTGC AGTCTGCGAG AAGGAACCCA GCTCCATCAT TGCTTTTGCT 
CTCAGTTGTA AAGAATACCG AAATGCCTTA GAGGAATTGT CTAAAGCGAC TCAGTGGAAC 
AGTGCCGAAG AAGGGCTTCC AACAAATAGT ACTTCAGATA GCAGACCAAA GAGTAGCAGC 
CCTATCAGAT TACCTGAAAT GAGTGGAGGA CAGACAAATC GTACAACAGA AACAGAACCA 
CAACCAACCA AAAAGGCTTC TGGAATGTTG TCCTTCTTCA GAGGGACAGC AGGGAAAAGC 
CCCGATCTCT CTTCCCAGAA GAGAGAGACC TTACGTGGAG CAGATAGTGC TTACTACCAG 
GTTGGGCAGA CGGGCAAGGA GGGGACCGAG AATCAAGGCG TTGAGCCTCA AGATGAAGTA 
GATGGAGGAG ATACACAAAA GAAGCAACTC ATAAATCCTC ATGTGGAACT TCAATTTTCA 
GATGCTAATG CCAAGTTTTA CTGTCGGCTC TACTATGCGG GAGAGTTTCA TAAGATGCGT 
GAAGTGATTC TGGACAGCAG TGAAGAAGAT TTCATTCGTT CCCTCTCCCA CTCATCACCC 
TGGCAGGCCC GGGGAGGCAA ATCAGGAGCT GCCTTCTATG CAACTGAGGA TGATAGATTT 
ATTTTGAAGC AAATGCCTCG TCTGGAAGTC CAGTCCTTCC TCGACTTTGC ACCACATTAC 
TTCAATTATA TTACAAATGC TGTTCAACAA AAGAGGCCCA CGGCGTTGGC CAAAATTCTT 
GGAGTTTACA GAATTGGTTA TAAGAACTCT CAGAACAACA CTGAGAAGAA GTTAGATCTC 
CTTGTCATGG AAAATCTTTT CTACGGGAGA AAGATGGCAC AGGTTTTTGA TTTGAAGGGC 
TCTCTTAGGA ATCGGAATGT AAAAACTGAC ACTGGAAAAG AGAGTTGTGA TGTGGTCCTG 
CTAGATGAAA ATCTCCTAAA GATGGTTCGA GACAACCCTC TATATATTCG TTCTCATTCC 
AAAGCTGTGC TGAGAACCTC GATCCATAGT GACTCCCATT TCCTTTCTAG CCACCTCATT 
ATAGATTATT CTTTGCTGGT TGGGCGAGAT GATACTAGCA ATGAGCTAGT AGTTGGAATT 
ATAGATTATA TTCGAACATT TACATGGGAC AAAAAGCTTG AGATGGTTGT GAAATCAACA 
GGAATTTTAG GTGGACAAGG TAAAATGCCA ACAGTGGTGT CTCCGGAGTT GTACAGGACT 
AGGTTTTGTG AGGCAATGGA CAAGTATTTC CTAATGGTAC CAGACCACTG GACAGGCTTG 
GGTCTGAATT GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011509083.1
CDS	182..6496
Translation	MATDDKTSPTLDSANDLPRSPTSPSHLTHFKPLTPDQDEPPFKSAYSSFVNLFRFNKERAEGGQGEQQPLSGSWTSPQLPSRTQSVRSPTPYKKQLNEELQRRSSALADNSLQHPQENTDTRRKAEPTFGGHDPRTAVQLRSLSTVLKRLKEIMEGKSQDSDLKQYWMPDSQCKECYDCSEKFTTFRRRHHCRLCGQIFCSRCCNQEIPGKFMGYTGDLRACTYCRKIALSYAHSTDSNSIGEDLNALSDSACSVSVLDPSEPRTPVGSRKASRNIFLEDDLAWQSLIHPDSSNTPLSTRLVSVQEDAGKSPARNRSASITNLSLDRSGSPMVPSYETSVSPQANRTYVRTETTEDERKILLDSVQLKDLWKKICHHSSGMEFQDHRYWLRTHPNCIVGKELVNWLIRNGHIATRAQAIAIGQAMVDGRWLDCVSHHDQLFRDEYALYRPLQSTEFSETPSPDSDSVNSVEGHSEPSWFKDIKFDDSDTEQIAEEDSASPSKRTSVSSFQSTVDSDSAASISLNVELDNVNFHIKKPSKYPHVPPHPADQKEYLISDTGGQQLSISDAFIKESLFNRRVEEKSKELPFTPLGWHHNNLELLREENGEKQAMERLLSANHNHMMALLQQLLHSDSLSSSWRDIIVSLVCQVVQTVRPDVKNQDDDMDIRQFVHIKKIPGGKKFDSVVVNGFVCTKNIAHKKMSSCIKNPKILLLKCSIEYLYREETKFTCIDPIVLQEREFLKNYVQRIVDVRPTLVLVEKTVSRIAQDMLLEHGITLVINVKSQVLERISRMTQGDLVMSMDQLLTKPHLGTCHKFYMQIFQLPNEQTKTLMFFEGCPQHLGCTIKLRGGSDYELARVKEILIFMICVAYHSQLEISFLMDEFAMPPTLMQNPSFHSLIEGRGHEGAVQEQYGGGSIPWDPDIPPESLPCDDSSLLELRIVFEKGEQENKNLPQAVASVKHQEHSTTACPAGLPCAFFAPVPESLLPLPVDDQQDALGSEQPETLQQTVVLQDPKSQIRAFRDPLQDDTGLYVTEEVTSSEDKRKTYSLAFKQELKDVILCISPVITFREPFLLTEKGMRCSTRDYFAEQVYWSPLLNKEFKEMENRRKKQLLRDLSGLQGMNGSIQAKSIQVLPSHELVSTRIAEHLGDSQSLGRMLADYRARGGRIQPKNSDPFAHSKDASSTSSGQSGSKNEGDEERGLILSDAVWSTKVDCLNPINHQRLCVLFSSSSAQSSNAPSACVSPWIVTMEFYGKNDLTLGIFLERYCFRPSYQCPSMFCDTPMVHHIRRFVHGQGCVQIILKELDSPVPGYQHTILTYSWCRICKQVTPVVALSNESWSMSFAKYLELRFYGHQYTRRANAEPCGHSIHHDYHQYFSYNQMVASFSYSPIRLLEVCVPLPKIFIKRQAPLKVSLLQDLKDFFQKVSQVYVAIDERLASLKTDTFSKTREEKMEDIFAQKEMEEGEFKNWIEKMQARLMSSSVDTPQQLQSVFESLIAKKQSLCEVLQAWNNRLQDLFQQEKGRKRPSVPPSPGRLRQGEESKISAMDASPRNISPGLQNGEKEDRFLTTLSSQSSTSSTHLQLPTPPEVMSEQSVGGPPELDTASSSEDVFDGHLLGSTDSQVKEKSTMKAIFANLLPGNSYNPIPFPFDPDKHYLMYEHERVPIAVCEKEPSSIIAFALSCKEYRNALEELSKATQWNSAEEGLPTNSTSDSRPKSSSPIRLPEMSGGQTNRTTETEPQPTKKASGMLSFFRGTAGKSPDLSSQKRETLRGADSAYYQVGQTGKEGTENQGVEPQDEVDGGDTQKKQLINPHVELQFSDANAKFYCRLYYAGEFHKMREVILDSSEEDFIRSLSHSSPWQARGGKSGAAFYATEDDRFILKQMPRLEVQSFLDFAPHYFNYITNAVQQKRPTALAKILGVYRIGYKNSQNNTEKKLDLLVMENLFYGRKMAQVFDLKGSLRNRNVKTDTGKESCDVVLLDENLLKMVRDNPLYIRSHSKAVLRTSIHSDSHFLSSHLIIDYSLLVGRDDTSNELVVGIIDYIRTFTWDKKLEMVVKSTGILGGQGKMPTVVSPELYRTRFCEAMDKYFLMVPDHWTGLGLNC

Target ORF information:

RefSeq Version	XM_011510781.3
Organism	Homo sapiens(human)
Definition	Homo sapiens phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011510781.3

ORF Insert Sequence:

ATGGCCACAG ATGATAAGAC GTCCCCAACA CTGGACTCTG CTAATGATTT GCCTCGATCT 
CCTACTAGTC CTTCTCATCT CACACACTTT AAACCTTTGA CTCCTGATCA AGATGAGCCC 
CCTTTTAAAT CAGCTTATAG TTCTTTTGTA AATCTCTTTC GTTTTAACAA AGAGAGAGCA 
GAAGGAGGCC AGGGAGAACA GCAGCCTTTG AGTGGAAGTT GGACCAGCCC TCAGCTCCCT 
TCGAGGACAC AGTCTGTTAG GTCACCCACA CCTTATAAAA AGCAGCTTAA TGAGGAACTC 
CAGCGGCGCT CTTCAGCATT AGCAGACAAC AGTTTGCAAC ATCCCCAGGA GAACACAGAC 
ACAAGAAGGA AAGCAGAACC TACCTTTGGA GGTCATGACC CTCGTACAGC TGTTCAGCTT 
CGAAGCCTCA GCACAGTATT AAAACGCCTC AAGGAAATCA TGGAGGGGAA AAGCCAGGAT 
AGTGACCTGA AACAATACTG GATGCCAGAT AGCCAATGTA AAGAGTGCTA TGACTGTAGT 
GAGAAATTTA CAACCTTTAG GCGCAGACAC CATTGCCGAC TATGTGGGCA GATTTTCTGC 
AGTCGTTGCT GTAATCAAGA AATCCCTGGA AAATTTATGG GCTATACAGG AGACCTCCGA 
GCTTGCACAT ATTGTAGAAA AATAGCCTTA AGTTATGCTC ATTCCACAGA CAGTAATTCT 
ATTGGGGAAG ACTTGAATGC TCTTTCAGAT TCTGCTTGCT CTGTGTCTGT GCTTGATCCA 
AGTGAACCCC GAACACCTGT TGGGAGTAGG AAAGCCAGCC GTAACATATT TTTAGAGGAT 
GATTTGGCCT GGCAAAGTTT GATTCATCCA GATTCCTCAA ATACTCCTCT TTCAACAAGA 
CTTGTATCTG TGCAAGAGGA TGCTGGGAAA TCTCCTGCTC GAAATAGATC AGCCAGCATT 
ACTAACCTGT CACTGGATAG ATCTGGTTCT CCTATGGTAC CTTCATATGA GACATCTGTC 
AGTCCCCAGG CTAACCGAAC ATATGTTAGG ACAGAGACCA CTGAGGATGA ACGCAAAATT 
CTTCTGGACA GTGTGCAGTT AAAAGACCTG TGGAAAAAAA TCTGCCATCA CAGCAGTGGA 
ATGGAGTTTC AGGATCACCG CTACTGGTTG AGAACGCATC CCAACTGCAT TGTAGGAAAG 
GAATTAGTCA ACTGGCTAAT CCGAAATGGG CATATTGCCA CAAGGGCACA AGCTATAGCA 
ATTGGACAAG CAATGGTTGA TGGACGTTGG CTGGATTGTG TTAGTCATCA CGACCAGCTT 
TTCAGAGATG AGTATGCGCT GTATAGACCA CTGCAGAGTA CAGAATTTTC TGAGACGCCT 
TCTCCCGACA GTGACTCAGT GAACTCCGTG GAAGGACACT CTGAGCCATC CTGGTTTAAA 
GACATAAAGT TTGATGACAG TGACACAGAA CAGATAGCTG AAGAAGATTC TGCCAGTCCT 
AGCAAGCGCA CATCAGTCAG CAGTTTCCAG TCCACAGTGG ACAGTGACTC AGCCGCTTCT 
ATCAGCCTGA ACGTGGAGCT GGACAACGTG AACTTCCATA TCAAGAAGCC CTCCAAGTAC 
CCACATGTGC CCCCTCACCC TGCTGACCAA AAAGAGTATT TGATTTCTGA CACTGGAGGA 
CAACAGCTCT CAATAAGTGA CGCTTTCATC AAAGAATCCT TATTTAATCG CCGAGTAGAG 
GAAAAATCCA AAGAGCTGCC TTTCACACCT TTGGGCTGGC ATCATAACAA CCTGGAGCTC 
CTGAGGGAGG AGAATGGGGA GAAACAAGCC ATGGAGAGGT TGCTTTCAGC TAATCATAAC 
CACATGATGG CACTACTCCA GCAGTTGCTC CATAGTGACT CACTGTCATC ATCTTGGAGG 
GACATCATCG TGTCATTGGT CTGCCAGGTT GTTCAGACAG TCCGACCTGA TGTCAAGAAC 
CAGGATGATG ACATGGATAT CCGTCAGTTT GTCCACATCA AAAAAATCCC AGGTGGAAAG 
AAGTTTGATT CTGTGGTTGT CAATGGCTTT GTTTGTACCA AGAACATTGC ACATAAAAAG 
ATGAGTTCTT GTATTAAAAA CCCCAAAATT CTTCTGTTGA AGTGTTCCAT TGAGTATCTC 
TACAGAGAAG AAACTAAGTT TACTTGCATT GATCCTATTG TGCTTCAGGA AAGGGAATTC 
TTGAAGAATT ATGTCCAGCG AATAGTTGAT GTTCGACCCA CCTTGGTTCT TGTTGAGAAA 
ACAGTGTCTC GGATTGCCCA GGACATGTTA TTGGAACATG GCATTACTTT GGTCATTAAT 
GTAAAGTCAC AAGTTTTGGA ACGAATCAGT CGAATGACCC AAGGTGATTT AGTGATGTCA 
ATGGACCAGC TGCTTACGAA ACCACACCTG GGCACTTGTC ACAAATTTTA TATGCAGATA 
TTTCAGTTGC CTAATGAACA AACCAAGACA CTGATGTTTT TTGAAGGTTG TCCACAGCAC 
CTAGGCTGTA CAATCAAGCT AAGAGGAGGC TCTGATTATG AGCTGGCTCG AGTTAAGGAG 
ATCCTAATAT TTATGATCTG TGTTGCTTAT CATTCTCAAC TAGAAATATC CTTTCTCATG 
GATGAATTTG CTATGCCTCC CACATTAATG CAAAACCCTT CATTCCATTC CCTGATTGAG 
GGACGAGGGC ATGAGGGGGC TGTCCAAGAG CAGTACGGTG GAGGTTCCAT CCCCTGGGAT 
CCTGACATCC CTCCTGAGTC TCTGCCCTGT GATGATAGCA GTTTGCTGGA ATTGAGGATT 
GTGTTTGAGA AGGGTGAGCA GGAAAATAAA AATCTTCCGC AGGCTGTTGC CTCTGTGAAG 
CATCAAGAAC ATAGCACAAC AGCTTGCCCG GCGGGTCTCC CTTGTGCTTT CTTTGCACCT 
GTACCGGAAT CATTGTTGCC ACTCCCTGTG GATGACCAAC AAGATGCTTT AGGCAGCGAG 
CAGCCAGAGA CTTTGCAGCA AACAGTTGTG CTGCAGGATC CCAAAAGCCA GATAAGAGCC 
TTTAGAGACC CTCTACAGGA TGACACTGGA TTATATGTTA CTGAGGAAGT CACCTCCTCT 
GAAGATAAAC GAAAGACTTA TTCTTTGGCC TTTAAGCAGG AATTAAAAGA TGTGATCCTC 
TGTATCTCCC CAGTAATCAC ATTCCGAGAA CCCTTTCTTT TAACTGAAAA GGGGATGAGA 
TGCTCTACCC GAGATTATTT TGCAGAGCAG GTTTACTGGT CTCCTCTCCT CAATAAAGAA 
TTCAAAGAAA TGGAGAACAG GAGGAAGAAA CAGCTGCTCA GGGATCTCTC TGGACTTCAG 
GGCATGAATG GAAGTATTCA GGCCAAGTCT ATTCAAGTCT TACCCTCACA TGAGCTAGTG 
AGCACTAGAA TTGCTGAGCA TCTGGGCGAT AGCCAGAGCT TGGGTAGAAT GCTGGCCGAT 
TATCGAGCCA GAGGAGGAAG AATTCAGCCC AAAAATTCAG ACCCTTTTGC TCATTCAAAG 
GATGCATCAA GTACTTCAAG TGGCCAATCA GGAAGCAAAA ATGAGGGTGA TGAAGAGAGA 
GGGCTTATTC TGAGTGATGC TGTGTGGTCA ACAAAGGTGG ACTGTCTGAA TCCCATTAAT 
CACCAGAGAC TTTGTGTGCT CTTCAGCAGC TCTTCTGCCC AGTCCAGCAA TGCTCCTAGT 
GCCTGTGTCA GTCCTTGGAT TGTAACAATG GAATTTTATG GAAAGAATGA TCTTACATTA 
GGAATATTTT TAGAGAGATA CTGTTTCAGG CCTTCTTATC AGTGTCCAAG CATGTTCTGT 
GATACCCCCA TGGTACATCA TATTCGGCGC TTTGTTCATG GCCAAGGCTG TGTGCAGATA 
ATCCTGAAGG AGTTGGATTC TCCAGTACCT GGATATCAGC ATACAATTCT TACATATTCC 
TGGTGTAGAA TCTGCAAACA GGTAACACCA GTTGTTGCTC TTTCCAATGA GTCCTGGTCT 
ATGTCATTTG CAAAATACCT TGAACTTAGG TTTTATGGGC ACCAGTATAC TCGCAGAGCC 
AACGCTGAGC CCTGTGGTCA CTCCATCCAT CATGATTATC ACCAGTATTT CTCCTATAAC 
CAGATGGTGG CGTCTTTCAG TTATTCTCCC ATTCGGCTTC TTGAAGTATG TGTTCCACTC 
CCCAAAATAT TCATTAAGCG TCAGGCCCCA TTAAAAGTGT CCCTTCTTCA GGATCTGAAG 
GACTTCTTTC AAAAAGTTTC ACAGGTATAT GTTGCCATTG ATGAAAGACT TGCATCTTTG 
AAAACTGATA CATTTAGTAA AACAAGAGAG GAAAAAATGG AAGATATTTT TGCACAGAAA 
GAGATGGAAG AAGGTGAGTT CAAGAACTGG ATTGAGAAGA TGCAAGCAAG GCTCATGTCT 
TCCTCTGTAG ATACCCCTCA GCAACTGCAG TCGGTCTTTG AGTCACTCAT TGCCAAGAAA 
CAAAGTCTCT GTGAAGTGCT GCAAGCTTGG AATAACAGGT TGCAGGACCT TTTCCAACAG 
GAAAAGGGTA GAAAGAGACC TTCAGTTCCT CCAAGTCCTG GAAGACTGAG ACAAGGGGAA 
GAAAGCAAGA TAAGTGCGAT GGATGCATCT CCACGGAATA TTTCTCCAGG ACTTCAGAAT 
GGAGAAAAAG AGGATCGCTT CTTAACAACT TTGTCCAGCC AGAGCTCCAC CAGTTCTACT 
CATCTCCAAT TGCCTACGCC ACCTGAAGTC ATGTCTGAAC AGTCAGTGGG AGGGCCCCCT 
GAGCTAGATA CAGCCAGCAG TTCCGAAGAT GTGTTTGATG GGCATTTGCT GGGATCCACA 
GACAGCCAAG TGAAGGAAAA GTCAACCATG AAAGCCATCT TTGCAAATTT GCTTCCAGGA 
AATAGCTATA ATCCTATTCC ATTTCCTTTT GATCCAGATA AACACTACTT AATGTATGAA 
CATGAACGAG TGCCCATTGC AGTCTGCGAG AAGGAACCCA GCTCCATCAT TGCTTTTGCT 
CTCAGTTGTA AAGAATACCG AAATGCCTTA GAGGAATTGT CTAAAGCGAC TCAGTGGAAC 
AGTGCCGAAG AAGGGCTTCC AACAAATAGT ACTTCAGATA GCAGACCAAA GAGTAGCAGC 
CCTATCAGAT TACCTGAAAT GAGTGGAGGA CAGACAAATC GTACAACAGA AACAGAACCA 
CAACCAACCA AAAAGGCTTC TGGAATGTTG TCCTTCTTCA GAGGGACAGC AGGGAAAAGC 
CCCGATCTCT CTTCCCAGAA GAGAGAGACC TTACGTGGAG CAGATAGTGC TTACTACCAG 
GTTGGGCAGA CGGGCAAGGA GGGGACCGAG AATCAAGGCG TTGAGCCTCA AGATGAAGTA 
GATGGAGGAG ATACACAAAA GAAGCAACTC ATAAATCCTC ATGTGGAACT TCAATTTTCA 
GATGCTAATG CCAAGTTTTA CTGTCGGCTC TACTATGCGG GAGAGTTTCA TAAGATGCGT 
GAAGTGATTC TGGACAGCAG TGAAGAAGAT TTCATTCGTT CCCTCTCCCA CTCATCACCC 
TGGCAGGCCC GGGGAGGCAA ATCAGGAGCT GCCTTCTATG CAACTGAGGA TGATAGATTT 
ATTTTGAAGC AAATGCCTCG TCTGGAAGTC CAGTCCTTCC TCGACTTTGC ACCACATTAC 
TTCAATTATA TTACAAATGC TGTTCAACAA AAGAGGCCCA CGGCGTTGGC CAAAATTCTT 
GGAGTTTACA GAATTGGTTA TAAGAACTCT CAGAACAACA CTGAGAAGAA GTTAGATCTC 
CTTGTCATGG AAAATCTTTT CTACGGGAGA AAGATGGCAC AGGTTTTTGA TTTGAAGGGC 
TCTCTTAGGA ATCGGAATGT AAAAACTGAC ACTGGAAAAG AGAGTTGTGA TGTGGTCCTG 
CTAGATGAAA ATCTCCTAAA GATGGTTCGA GACAACCCTC TATATATTCG TTCTCATTCC 
AAAGCTGTGC TGAGAACCTC GATCCATAGT GACTCCCATT TCCTTTCTAG CCACCTCATT 
ATAGATTATT CTTTGCTGGT TGGGCGAGAT GATACTAGCA ATGAGCTAGT AGTTGGAATT 
ATAGATTATA TTCGAACATT TACATGGGAC AAAAAGCTTG AGATGGTTGT GAAATCAACA 
GGAATTTTAG GTGGACAAGG TAAAATGCCA ACAGTGGTGT CTCCGGAGTT GTACAGGACT 
AGGTTTTGTG AGGCAATGGA CAAGTATTTC CTAATGGTAC CAGACCACTG GACAGGCTTG 
GGTCTGAATT GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PIKFYVE ( XM_011510782.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011509084.1 Homo sapiens phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X6, mRNA.

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CloneID	OHu77664
Clone ID Related Accession (Same CDS sequence)	XM_011510782.2 , XM_011510782.3
Accession Version	XM_011510782.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6168bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	1-phosphatidylinositol 3-phosphate 5-kinase isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011510782.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG ATGATAAGAC GTCCCCAACA CTGGACTCTG CTAATGATTT GCCTCGATCT 
CCTACTAGTC CTTCTCATCT CACACACTTT AAACCTTTGA CTCCTGATCA AGATGAGCCC 
CCTTTTAAAT CAGCTTATAG TTCTTTTGTA AATCTCTTTC GTTTTAACAA AGAGAGAGCA 
GAAGGAGGCC AGGGAGAACA GCAGCCTTTG AGTGGAAGTT GGACCAGCCC TCAGCTCCCT 
TCGAGGACAC AGTCTGTTAG GTCACCCACA CCTTATAAAA AGCAGCTTAA TGAGGAACTC 
CAGCGGCGCT CTTCAGCATT AGCAGACAAC AGTTTGCAAC ATCCCCAGGA GAACACAGAC 
ACAAGAAGGA AAGCAGAACC TACCTTTGGA GGTCATGACC CTCGTACAGC TGTTCAGCTT 
CGAAGCCTCA GCACAGTATT AAAACGCCTC AAGGAAATCA TGGAGGGGAA AAGCCAGGAT 
AGTGACCTGA AACAATACTG GATGCCAGAT AGCCAATGTA AAGAGTGCTA TGACTGTAGT 
GAGAAATTTA CAACCTTTAG GCGCAGACAC CATTGCCGAC TATGTGGGCA GATTTTCTGC 
AGTCGTTGCT GTAATCAAGA AATCCCTGGA AAATTTATGG GCTATACAGG AGACCTCCGA 
GCTTGCACAT ATTGTAGAAA AATAGCCTTA AGTTATGCTC ATTCCACAGA CAGTAATTCT 
ATTGGGGAAG ACTTGAATGC TCTTTCAGAT TCTGCTTGCT CTGTGTCTGT GCTTGATCCA 
AGTGAACCCC GAACACCTGT TGGGAGTAGG AAAGCCAGCC GTAACATATT TTTAGAGGAT 
GATTTGGCCT GGCAAAGTTT GATTCATCCA GATTCCTCAA ATACTCCTCT TTCAACAAGA 
CTTGTATCTG TGCAAGAGGA TGCTGGGAAA TCTCCTGCTC GAAATAGATC AGCCAGCATT 
ACTAACCTGT CACTGGATAG ATCTGGTTCT CCTATGGTAC CTTCATATGA GACATCTGTC 
AGTCCCCAGG CTAACCGAAC ATATGTTAGG ACAGAGACCA CTGAGGATGA ACGCAAAATT 
CTTCTGGACA GTGTGCAGTT AAAAGACCTG TGGAAAAAAA TCTGCCATCA CAGCAGTGGA 
ATGGAGTTTC AGGATCACCG CTACTGGTTG AGAACGCATC CCAACTGCAT TGTAGGAAAG 
GAATTAGTCA ACTGGCTAAT CCGAAATGGG CATATTGCCA CAAGGGCACA AGCTATAGCA 
ATTGGACAAG CAATGGTTGA TGGACGTTGG CTGGATTGTG TTAGTCATCA CGACCAGCTT 
TTCAGAGATG AGTATGCGCT GTATAGACCA CTGCAGAGTA CAGAATTTTC TGAGACGCCT 
TCTCCCGACA GTGACTCAGT GAACTCCGTG GAAGGACACT CTGAGCCATC CTGGTTTAAA 
GACATAAAGT TTGATGACAG TGACACAGAA CAGATAGCTG AAGAAGGTGA CGATAATTTG 
GCTAATTCTG CCAGTCCTAG CAAGCGCACA TCAGTCAGCA GTTTCCAGTC CACAGTGGAC 
AGTGACTCAG CCGCTTCTAT CAGCCTGAAC GTGGAGCTGG ACAACGTGAA CTTCCATATC 
AAGAAGCCCT CCAAGTACCC ACATGTGCCC CCTCACCCTG CTGACCAAAA AGAGTATTTG 
ATTTCTGACA CTGGAGGACA ACAGCTCTCA ATAAGTGACG CTTTCATCAA AGAATCCTTA 
TTTAATCGCC GAGTAGAGGA AAAATCCAAA GAGCTGCCTT TCACACCTTT GGGCTGGCAT 
CATAACAACC TGGAGCTCCT GAGGGAGGAG AATGGGGAGA AACAAGCCAT GGAGAGGTTG 
CTTTCAGCTA ATCATAACCA CATGATGGCA CTACTCCAGC AGTTGCTCCA TAGTGACTCA 
CTGTCATCAT CTTGGAGGGA CATCATCGTG TCATTGGTCT GCCAGGTTGT TCAGACAGTC 
CGACCTGATG TCAAGAACCA GGATGATGAC ATGGATATCC GTCAGTTTGT CCACATCAAA 
AAAATCCCAG GTGGAAAGAA GTTTGATTCT GTGGTTGTCA ATGGCTTTGT TTGTACCAAG 
AACATTGCAC ATAAAAAGAT GAGTTCTTGT ATTAAAAACC CCAAAATTCT TCTGTTGAAG 
TGTTCCATTG AGTATCTCTA CAGAGAAGAA ACTAAGTTTA CTTGCATTGA TCCTATTGTG 
CTTCAGGAAA GGGAATTCTT GAAGAATTAT GTCCAGCGAA TAGTTGATGT TCGACCCACC 
TTGGTTCTTG TTGAGAAAAC AGTGTCTCGG ATTGCCCAGG ACATGTTATT GGAACATGGC 
ATTACTTTGG TCATTAATGT AAAGTCACAA GTTTTGGAAC GAATCAGTCG AATGACCCAA 
GGTGATTTAG TGATGTCAAT GGACCAGCTG CTTACGAAAC CACACCTGGG CACTTGTCAC 
AAATTTTATA TGCAGATATT TCAGTTGCCT AATGAACAAA CCAAGACACT GATGTTTTTT 
GAAGGTTGTC CACAGCACCT AGGCTGTACA ATCAAGCTAA GAGGAGGCTC TGATTATGAG 
CTGGCTCGAG TTAAGGAGAT CCTAATATTT ATGATCTGTG TTGCTTATCA TTCTCAACTA 
GAAATATCCT TTCTCATGGA TGAATTTGCT ATGCCTCCCA CATTAATGCA AAACCCTTCA 
TTCCATTCCC TGATTGAGGG ACGAGGGCAT GAGGGGGCTG TCCAAGAGCA GTACGGTGGA 
GGTTCCATCC CCTGGGATCC TGACATCCCT CCTGAGTCTC TGCCCTGTGA TGATAGCAGT 
TTGCTGGAAT TGAGGATTGT GTTTGAGAAG GGTGAGCAGG AAAATAAAAA TCTTCCGCAG 
GCTGTTGCCT CTGTGAAGCA TCAAGAACAT AGCACAACAG CTTGCCCGGC GGGTCTCCCT 
TGTGCTTTCT TTGCACCTGT ACCGGAATCA TTGTTGCCAC TCCCTGTGGA TGACCAACAA 
GATGCTTTAG GCAGCGAGCA GCCAGAGACT TTGCAGCAAA CAGTTGTGCT GCAGGATCCC 
AAAAGCCAGA TAAGAGCCTT TAGAGACCCT CTACAGGATG ACACTGGATT ATATGTTACT 
GAGGAAGTCA CCTCCTCTGA AGATAAACGA AAGACTTATT CTTTGGCCTT TAAGCAGGAA 
TTAAAAGATG TGATCCTCTG TATCTCCCCA GTAATCACAT TCCGAGAACC CTTTCTTTTA 
ACTGAAAAGG GGATGAGATG CTCTACCCGA GATTATTTTG CAGAGCAGGT TTACTGGTCT 
CCTCTCCTCA ATAAAGAATT CAAAGAAATG GAGAACAGGA GGAAGAAACA GCTGCTCAGG 
GATCTCTCTG GACTTCAGGG CATGAATGGA AGTATTCAGG CCAAGTCTAT TCAAGTCTTA 
CCCTCACATG AGCTAGTGAG CACTAGAATT GCTGAGCATC TGGGCGATAG CCAGAGCTTG 
GGTAGAATGC TGGCCGATTA TCGAGCCAGA GGAGGAAGAA TTCAGCCCAA AAATTCAGAC 
CCTTTTGCTC ATTCAAAGGA TGCATCAAGT ACTTCAAGTG GCCAATCAGG AAGCAAAAAT 
GAGGGTGATG AAGAGAGAGG GCTTATTCTG AGTGATGCTG TGTGGTCAAC AAAGGTGGAC 
TGTCTGAATC CCATTAATCA CCAGAGACTT TGTGTGCTCT TCAGCAGCTC TTCTGCCCAG 
TCCAGCAATG CTCCTAGTGC CTGTGTCAGT CCTTGGATTG TAACAATGGA ATTTTATGGA 
AAGAATGATC TTACATTAGG AATATTTTTA GAGAGATACT GTTTCAGGCC TTCTTATCAG 
TGTCCAAGCA TGTTCTGTGA TACCCCCATG GTACATCATA TTCGGCGCTT TGTTCATGGC 
CAAGGCTGTG TGCAGATAAT CCTGAAGGAG TTGGATTCTC CAGTACCTGG ATATCAGCAT 
ACAATTCTTA CATATTCCTG GTGTAGAATC TGCAAACAGG TAACACCAGT TGTTGCTCTT 
TCCAATGAGT CCTGGTCTAT GTCATTTGCA AAATACCTTG AACTTAGGTT TTATGGGCAC 
CAGTATACTC GCAGAGCCAA CGCTGAGCCC TGTGGTCACT CCATCCATCA TGATTATCAC 
CAGTATTTCT CCTATAACCA GATGGTGGCG TCTTTCAGTT ATTCTCCCAT TCGGCTTCTT 
GAAGTATGTG TTCCACTCCC CAAAATATTC ATTAAGCGTC AGGCCCCATT AAAAGTGTCC 
CTTCTTCAGG ATCTGAAGGA CTTCTTTCAA AAAGTTTCAC AGGTATATGT TGCCATTGAT 
GAAAGACTTG CATCTTTGAA AACTGATACA TTTAGTAAAA CAAGAGAGGA AAAAATGGAA 
GATATTTTTG CACAGAAAGA GATGGAAGAA GGTGAGTTCA AGAACTGGAT TGAGAAGATG 
CAAGCAAGGC TCATGTCTTC CTCTGTAGAT ACCCCTCAGC AACTGCAGTC GGTCTTTGAG 
TCACTCATTG CCAAGAAACA AAGTCTCTGT GAAGTGCTGC AAGCTTGGAA TAACAGGTTG 
CAGGACCTTT TCCAACAGGA AAAGGGTAGA AAGAGACCTT CAGTTCCTCC AAGTCCTGGA 
AGACTGAGAC AAGGGGAAGA AAGCAAGATA AGTGCGATGG ATGCATCTCC ACGGAATATT 
TCTCCAGGAC TTCAGAATGG AGAAAAAGAG GATCGCTTCT TAACAACTTT GTCCAGCCAG 
AGCTCCACCA GTTCTACTCA TCTCCAATTG CCTACGCCAC CTGAAGTCAT GTCTGAACAG 
TCAGTGGGAG GGCCCCCTGA GCTAGATACA GCCAGCAGTT CCGAAGATGT GTTTGATGGG 
CATTTGCTGG GATCCACAGA CAGCCAAGTG AAGGAAAAGT CAACCATGAA AGCCATCTTT 
GCAAATTTGC TTCCAGGAAA TAGCTATAAT CCTATTCCAT TTCCTTTTGA TCCAGATAAA 
CACTACTTAA TGTATGAACA TGAACGAGTG CCCATTGCAG TCTGCGAGAA GGAACCCAGC 
TCCATCATTG CTTTTGCTCT CAGTTGTAAA GAATACCGAA ATGCCTTAGA GGAATTGTCT 
AAAGCGACTC AGTGGAACAG TGCCGAAGAA GGGCTTCCAA CAAATAGTAC TTCAGATAGC 
AGACCAAAGA GTAGCAGCCC TATCAGATTA CCTGAAATGA GTGGAGGACA GACAAATCGT 
ACAACAGAAA CAGAACCACA ACCAAATGAA GTAGATGGAG GAGATACACA AAAGAAGCAA 
CTCATAAATC CTCATGTGGA ACTTCAATTT TCAGATGCTA ATGCCAAGTT TTACTGTCGG 
CTCTACTATG CGGGAGAGTT TCATAAGATG CGTGAAGTGA TTCTGGACAG CAGTGAAGAA 
GATTTCATTC GTTCCCTCTC CCACTCATCA CCCTGGCAGG CCCGGGGAGG CAAATCAGGA 
GCTGCCTTCT ATGCAACTGA GGATGATAGA TTTATTTTGA AGCAAATGCC TCGTCTGGAA 
GTCCAGTCCT TCCTCGACTT TGCACCACAT TACTTCAATT ATATTACAAA TGCTGTTCAA 
CAAAAGAGGC CCACGGCGTT GGCCAAAATT CTTGGAGTTT ACAGAATTGG TTATAAGAAC 
TCTCAGAACA ACACTGAGAA GAAGTTAGAT CTCCTTGTCA TGGAAAATCT TTTCTACGGG 
AGAAAGATGG CACAGGTTTT TGATTTGAAG GGCTCTCTTA GGAATCGGAA TGTAAAAACT 
GACACTGGAA AAGAGAGTTG TGATGTGGTC CTGCTAGATG AAAATCTCCT AAAGATGGTT 
CGAGACAACC CTCTATATAT TCGTTCTCAT TCCAAAGCTG TGCTGAGAAC CTCGATCCAT 
AGTGACTCCC ATTTCCTTTC TAGCCACCTC ATTATAGATT ATTCTTTGCT GGTTGGGCGA 
GATGATACTA GCAATGAGCT AGTAGTTGGA ATTATAGATT ATATTCGAAC ATTTACATGG 
GACAAAAAGC TTGAGATGGT TGTGAAATCA ACAGGAATTT TAGGTGGACA AGGTAAAATG 
CCAACAGTGG TGTCTCCGGA GTTGTACAGG ACTAGGTTTT GTGAGGCAAT GGACAAGTAT 
TTCCTAATGG TACCAGACCA CTGGACAGGC TTGGGTCTGA ATTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011509084.1
CDS	173..6340
Translation	MATDDKTSPTLDSANDLPRSPTSPSHLTHFKPLTPDQDEPPFKSAYSSFVNLFRFNKERAEGGQGEQQPLSGSWTSPQLPSRTQSVRSPTPYKKQLNEELQRRSSALADNSLQHPQENTDTRRKAEPTFGGHDPRTAVQLRSLSTVLKRLKEIMEGKSQDSDLKQYWMPDSQCKECYDCSEKFTTFRRRHHCRLCGQIFCSRCCNQEIPGKFMGYTGDLRACTYCRKIALSYAHSTDSNSIGEDLNALSDSACSVSVLDPSEPRTPVGSRKASRNIFLEDDLAWQSLIHPDSSNTPLSTRLVSVQEDAGKSPARNRSASITNLSLDRSGSPMVPSYETSVSPQANRTYVRTETTEDERKILLDSVQLKDLWKKICHHSSGMEFQDHRYWLRTHPNCIVGKELVNWLIRNGHIATRAQAIAIGQAMVDGRWLDCVSHHDQLFRDEYALYRPLQSTEFSETPSPDSDSVNSVEGHSEPSWFKDIKFDDSDTEQIAEEGDDNLANSASPSKRTSVSSFQSTVDSDSAASISLNVELDNVNFHIKKPSKYPHVPPHPADQKEYLISDTGGQQLSISDAFIKESLFNRRVEEKSKELPFTPLGWHHNNLELLREENGEKQAMERLLSANHNHMMALLQQLLHSDSLSSSWRDIIVSLVCQVVQTVRPDVKNQDDDMDIRQFVHIKKIPGGKKFDSVVVNGFVCTKNIAHKKMSSCIKNPKILLLKCSIEYLYREETKFTCIDPIVLQEREFLKNYVQRIVDVRPTLVLVEKTVSRIAQDMLLEHGITLVINVKSQVLERISRMTQGDLVMSMDQLLTKPHLGTCHKFYMQIFQLPNEQTKTLMFFEGCPQHLGCTIKLRGGSDYELARVKEILIFMICVAYHSQLEISFLMDEFAMPPTLMQNPSFHSLIEGRGHEGAVQEQYGGGSIPWDPDIPPESLPCDDSSLLELRIVFEKGEQENKNLPQAVASVKHQEHSTTACPAGLPCAFFAPVPESLLPLPVDDQQDALGSEQPETLQQTVVLQDPKSQIRAFRDPLQDDTGLYVTEEVTSSEDKRKTYSLAFKQELKDVILCISPVITFREPFLLTEKGMRCSTRDYFAEQVYWSPLLNKEFKEMENRRKKQLLRDLSGLQGMNGSIQAKSIQVLPSHELVSTRIAEHLGDSQSLGRMLADYRARGGRIQPKNSDPFAHSKDASSTSSGQSGSKNEGDEERGLILSDAVWSTKVDCLNPINHQRLCVLFSSSSAQSSNAPSACVSPWIVTMEFYGKNDLTLGIFLERYCFRPSYQCPSMFCDTPMVHHIRRFVHGQGCVQIILKELDSPVPGYQHTILTYSWCRICKQVTPVVALSNESWSMSFAKYLELRFYGHQYTRRANAEPCGHSIHHDYHQYFSYNQMVASFSYSPIRLLEVCVPLPKIFIKRQAPLKVSLLQDLKDFFQKVSQVYVAIDERLASLKTDTFSKTREEKMEDIFAQKEMEEGEFKNWIEKMQARLMSSSVDTPQQLQSVFESLIAKKQSLCEVLQAWNNRLQDLFQQEKGRKRPSVPPSPGRLRQGEESKISAMDASPRNISPGLQNGEKEDRFLTTLSSQSSTSSTHLQLPTPPEVMSEQSVGGPPELDTASSSEDVFDGHLLGSTDSQVKEKSTMKAIFANLLPGNSYNPIPFPFDPDKHYLMYEHERVPIAVCEKEPSSIIAFALSCKEYRNALEELSKATQWNSAEEGLPTNSTSDSRPKSSSPIRLPEMSGGQTNRTTETEPQPNEVDGGDTQKKQLINPHVELQFSDANAKFYCRLYYAGEFHKMREVILDSSEEDFIRSLSHSSPWQARGGKSGAAFYATEDDRFILKQMPRLEVQSFLDFAPHYFNYITNAVQQKRPTALAKILGVYRIGYKNSQNNTEKKLDLLVMENLFYGRKMAQVFDLKGSLRNRNVKTDTGKESCDVVLLDENLLKMVRDNPLYIRSHSKAVLRTSIHSDSHFLSSHLIIDYSLLVGRDDTSNELVVGIIDYIRTFTWDKKLEMVVKSTGILGGQGKMPTVVSPELYRTRFCEAMDKYFLMVPDHWTGLGLNC

Target ORF information:

RefSeq Version	XM_011510782.2
Organism	Homo sapiens(human)
Definition	Homo sapiens phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011510782.2

ORF Insert Sequence:

ATGGCCACAG ATGATAAGAC GTCCCCAACA CTGGACTCTG CTAATGATTT GCCTCGATCT 
CCTACTAGTC CTTCTCATCT CACACACTTT AAACCTTTGA CTCCTGATCA AGATGAGCCC 
CCTTTTAAAT CAGCTTATAG TTCTTTTGTA AATCTCTTTC GTTTTAACAA AGAGAGAGCA 
GAAGGAGGCC AGGGAGAACA GCAGCCTTTG AGTGGAAGTT GGACCAGCCC TCAGCTCCCT 
TCGAGGACAC AGTCTGTTAG GTCACCCACA CCTTATAAAA AGCAGCTTAA TGAGGAACTC 
CAGCGGCGCT CTTCAGCATT AGCAGACAAC AGTTTGCAAC ATCCCCAGGA GAACACAGAC 
ACAAGAAGGA AAGCAGAACC TACCTTTGGA GGTCATGACC CTCGTACAGC TGTTCAGCTT 
CGAAGCCTCA GCACAGTATT AAAACGCCTC AAGGAAATCA TGGAGGGGAA AAGCCAGGAT 
AGTGACCTGA AACAATACTG GATGCCAGAT AGCCAATGTA AAGAGTGCTA TGACTGTAGT 
GAGAAATTTA CAACCTTTAG GCGCAGACAC CATTGCCGAC TATGTGGGCA GATTTTCTGC 
AGTCGTTGCT GTAATCAAGA AATCCCTGGA AAATTTATGG GCTATACAGG AGACCTCCGA 
GCTTGCACAT ATTGTAGAAA AATAGCCTTA AGTTATGCTC ATTCCACAGA CAGTAATTCT 
ATTGGGGAAG ACTTGAATGC TCTTTCAGAT TCTGCTTGCT CTGTGTCTGT GCTTGATCCA 
AGTGAACCCC GAACACCTGT TGGGAGTAGG AAAGCCAGCC GTAACATATT TTTAGAGGAT 
GATTTGGCCT GGCAAAGTTT GATTCATCCA GATTCCTCAA ATACTCCTCT TTCAACAAGA 
CTTGTATCTG TGCAAGAGGA TGCTGGGAAA TCTCCTGCTC GAAATAGATC AGCCAGCATT 
ACTAACCTGT CACTGGATAG ATCTGGTTCT CCTATGGTAC CTTCATATGA GACATCTGTC 
AGTCCCCAGG CTAACCGAAC ATATGTTAGG ACAGAGACCA CTGAGGATGA ACGCAAAATT 
CTTCTGGACA GTGTGCAGTT AAAAGACCTG TGGAAAAAAA TCTGCCATCA CAGCAGTGGA 
ATGGAGTTTC AGGATCACCG CTACTGGTTG AGAACGCATC CCAACTGCAT TGTAGGAAAG 
GAATTAGTCA ACTGGCTAAT CCGAAATGGG CATATTGCCA CAAGGGCACA AGCTATAGCA 
ATTGGACAAG CAATGGTTGA TGGACGTTGG CTGGATTGTG TTAGTCATCA CGACCAGCTT 
TTCAGAGATG AGTATGCGCT GTATAGACCA CTGCAGAGTA CAGAATTTTC TGAGACGCCT 
TCTCCCGACA GTGACTCAGT GAACTCCGTG GAAGGACACT CTGAGCCATC CTGGTTTAAA 
GACATAAAGT TTGATGACAG TGACACAGAA CAGATAGCTG AAGAAGGTGA CGATAATTTG 
GCTAATTCTG CCAGTCCTAG CAAGCGCACA TCAGTCAGCA GTTTCCAGTC CACAGTGGAC 
AGTGACTCAG CCGCTTCTAT CAGCCTGAAC GTGGAGCTGG ACAACGTGAA CTTCCATATC 
AAGAAGCCCT CCAAGTACCC ACATGTGCCC CCTCACCCTG CTGACCAAAA AGAGTATTTG 
ATTTCTGACA CTGGAGGACA ACAGCTCTCA ATAAGTGACG CTTTCATCAA AGAATCCTTA 
TTTAATCGCC GAGTAGAGGA AAAATCCAAA GAGCTGCCTT TCACACCTTT GGGCTGGCAT 
CATAACAACC TGGAGCTCCT GAGGGAGGAG AATGGGGAGA AACAAGCCAT GGAGAGGTTG 
CTTTCAGCTA ATCATAACCA CATGATGGCA CTACTCCAGC AGTTGCTCCA TAGTGACTCA 
CTGTCATCAT CTTGGAGGGA CATCATCGTG TCATTGGTCT GCCAGGTTGT TCAGACAGTC 
CGACCTGATG TCAAGAACCA GGATGATGAC ATGGATATCC GTCAGTTTGT CCACATCAAA 
AAAATCCCAG GTGGAAAGAA GTTTGATTCT GTGGTTGTCA ATGGCTTTGT TTGTACCAAG 
AACATTGCAC ATAAAAAGAT GAGTTCTTGT ATTAAAAACC CCAAAATTCT TCTGTTGAAG 
TGTTCCATTG AGTATCTCTA CAGAGAAGAA ACTAAGTTTA CTTGCATTGA TCCTATTGTG 
CTTCAGGAAA GGGAATTCTT GAAGAATTAT GTCCAGCGAA TAGTTGATGT TCGACCCACC 
TTGGTTCTTG TTGAGAAAAC AGTGTCTCGG ATTGCCCAGG ACATGTTATT GGAACATGGC 
ATTACTTTGG TCATTAATGT AAAGTCACAA GTTTTGGAAC GAATCAGTCG AATGACCCAA 
GGTGATTTAG TGATGTCAAT GGACCAGCTG CTTACGAAAC CACACCTGGG CACTTGTCAC 
AAATTTTATA TGCAGATATT TCAGTTGCCT AATGAACAAA CCAAGACACT GATGTTTTTT 
GAAGGTTGTC CACAGCACCT AGGCTGTACA ATCAAGCTAA GAGGAGGCTC TGATTATGAG 
CTGGCTCGAG TTAAGGAGAT CCTAATATTT ATGATCTGTG TTGCTTATCA TTCTCAACTA 
GAAATATCCT TTCTCATGGA TGAATTTGCT ATGCCTCCCA CATTAATGCA AAACCCTTCA 
TTCCATTCCC TGATTGAGGG ACGAGGGCAT GAGGGGGCTG TCCAAGAGCA GTACGGTGGA 
GGTTCCATCC CCTGGGATCC TGACATCCCT CCTGAGTCTC TGCCCTGTGA TGATAGCAGT 
TTGCTGGAAT TGAGGATTGT GTTTGAGAAG GGTGAGCAGG AAAATAAAAA TCTTCCGCAG 
GCTGTTGCCT CTGTGAAGCA TCAAGAACAT AGCACAACAG CTTGCCCGGC GGGTCTCCCT 
TGTGCTTTCT TTGCACCTGT ACCGGAATCA TTGTTGCCAC TCCCTGTGGA TGACCAACAA 
GATGCTTTAG GCAGCGAGCA GCCAGAGACT TTGCAGCAAA CAGTTGTGCT GCAGGATCCC 
AAAAGCCAGA TAAGAGCCTT TAGAGACCCT CTACAGGATG ACACTGGATT ATATGTTACT 
GAGGAAGTCA CCTCCTCTGA AGATAAACGA AAGACTTATT CTTTGGCCTT TAAGCAGGAA 
TTAAAAGATG TGATCCTCTG TATCTCCCCA GTAATCACAT TCCGAGAACC CTTTCTTTTA 
ACTGAAAAGG GGATGAGATG CTCTACCCGA GATTATTTTG CAGAGCAGGT TTACTGGTCT 
CCTCTCCTCA ATAAAGAATT CAAAGAAATG GAGAACAGGA GGAAGAAACA GCTGCTCAGG 
GATCTCTCTG GACTTCAGGG CATGAATGGA AGTATTCAGG CCAAGTCTAT TCAAGTCTTA 
CCCTCACATG AGCTAGTGAG CACTAGAATT GCTGAGCATC TGGGCGATAG CCAGAGCTTG 
GGTAGAATGC TGGCCGATTA TCGAGCCAGA GGAGGAAGAA TTCAGCCCAA AAATTCAGAC 
CCTTTTGCTC ATTCAAAGGA TGCATCAAGT ACTTCAAGTG GCCAATCAGG AAGCAAAAAT 
GAGGGTGATG AAGAGAGAGG GCTTATTCTG AGTGATGCTG TGTGGTCAAC AAAGGTGGAC 
TGTCTGAATC CCATTAATCA CCAGAGACTT TGTGTGCTCT TCAGCAGCTC TTCTGCCCAG 
TCCAGCAATG CTCCTAGTGC CTGTGTCAGT CCTTGGATTG TAACAATGGA ATTTTATGGA 
AAGAATGATC TTACATTAGG AATATTTTTA GAGAGATACT GTTTCAGGCC TTCTTATCAG 
TGTCCAAGCA TGTTCTGTGA TACCCCCATG GTACATCATA TTCGGCGCTT TGTTCATGGC 
CAAGGCTGTG TGCAGATAAT CCTGAAGGAG TTGGATTCTC CAGTACCTGG ATATCAGCAT 
ACAATTCTTA CATATTCCTG GTGTAGAATC TGCAAACAGG TAACACCAGT TGTTGCTCTT 
TCCAATGAGT CCTGGTCTAT GTCATTTGCA AAATACCTTG AACTTAGGTT TTATGGGCAC 
CAGTATACTC GCAGAGCCAA CGCTGAGCCC TGTGGTCACT CCATCCATCA TGATTATCAC 
CAGTATTTCT CCTATAACCA GATGGTGGCG TCTTTCAGTT ATTCTCCCAT TCGGCTTCTT 
GAAGTATGTG TTCCACTCCC CAAAATATTC ATTAAGCGTC AGGCCCCATT AAAAGTGTCC 
CTTCTTCAGG ATCTGAAGGA CTTCTTTCAA AAAGTTTCAC AGGTATATGT TGCCATTGAT 
GAAAGACTTG CATCTTTGAA AACTGATACA TTTAGTAAAA CAAGAGAGGA AAAAATGGAA 
GATATTTTTG CACAGAAAGA GATGGAAGAA GGTGAGTTCA AGAACTGGAT TGAGAAGATG 
CAAGCAAGGC TCATGTCTTC CTCTGTAGAT ACCCCTCAGC AACTGCAGTC GGTCTTTGAG 
TCACTCATTG CCAAGAAACA AAGTCTCTGT GAAGTGCTGC AAGCTTGGAA TAACAGGTTG 
CAGGACCTTT TCCAACAGGA AAAGGGTAGA AAGAGACCTT CAGTTCCTCC AAGTCCTGGA 
AGACTGAGAC AAGGGGAAGA AAGCAAGATA AGTGCGATGG ATGCATCTCC ACGGAATATT 
TCTCCAGGAC TTCAGAATGG AGAAAAAGAG GATCGCTTCT TAACAACTTT GTCCAGCCAG 
AGCTCCACCA GTTCTACTCA TCTCCAATTG CCTACGCCAC CTGAAGTCAT GTCTGAACAG 
TCAGTGGGAG GGCCCCCTGA GCTAGATACA GCCAGCAGTT CCGAAGATGT GTTTGATGGG 
CATTTGCTGG GATCCACAGA CAGCCAAGTG AAGGAAAAGT CAACCATGAA AGCCATCTTT 
GCAAATTTGC TTCCAGGAAA TAGCTATAAT CCTATTCCAT TTCCTTTTGA TCCAGATAAA 
CACTACTTAA TGTATGAACA TGAACGAGTG CCCATTGCAG TCTGCGAGAA GGAACCCAGC 
TCCATCATTG CTTTTGCTCT CAGTTGTAAA GAATACCGAA ATGCCTTAGA GGAATTGTCT 
AAAGCGACTC AGTGGAACAG TGCCGAAGAA GGGCTTCCAA CAAATAGTAC TTCAGATAGC 
AGACCAAAGA GTAGCAGCCC TATCAGATTA CCTGAAATGA GTGGAGGACA GACAAATCGT 
ACAACAGAAA CAGAACCACA ACCAAATGAA GTAGATGGAG GAGATACACA AAAGAAGCAA 
CTCATAAATC CTCATGTGGA ACTTCAATTT TCAGATGCTA ATGCCAAGTT TTACTGTCGG 
CTCTACTATG CGGGAGAGTT TCATAAGATG CGTGAAGTGA TTCTGGACAG CAGTGAAGAA 
GATTTCATTC GTTCCCTCTC CCACTCATCA CCCTGGCAGG CCCGGGGAGG CAAATCAGGA 
GCTGCCTTCT ATGCAACTGA GGATGATAGA TTTATTTTGA AGCAAATGCC TCGTCTGGAA 
GTCCAGTCCT TCCTCGACTT TGCACCACAT TACTTCAATT ATATTACAAA TGCTGTTCAA 
CAAAAGAGGC CCACGGCGTT GGCCAAAATT CTTGGAGTTT ACAGAATTGG TTATAAGAAC 
TCTCAGAACA ACACTGAGAA GAAGTTAGAT CTCCTTGTCA TGGAAAATCT TTTCTACGGG 
AGAAAGATGG CACAGGTTTT TGATTTGAAG GGCTCTCTTA GGAATCGGAA TGTAAAAACT 
GACACTGGAA AAGAGAGTTG TGATGTGGTC CTGCTAGATG AAAATCTCCT AAAGATGGTT 
CGAGACAACC CTCTATATAT TCGTTCTCAT TCCAAAGCTG TGCTGAGAAC CTCGATCCAT 
AGTGACTCCC ATTTCCTTTC TAGCCACCTC ATTATAGATT ATTCTTTGCT GGTTGGGCGA 
GATGATACTA GCAATGAGCT AGTAGTTGGA ATTATAGATT ATATTCGAAC ATTTACATGG 
GACAAAAAGC TTGAGATGGT TGTGAAATCA ACAGGAATTT TAGGTGGACA AGGTAAAATG 
CCAACAGTGG TGTCTCCGGA GTTGTACAGG ACTAGGTTTT GTGAGGCAAT GGACAAGTAT 
TTCCTAATGG TACCAGACCA CTGGACAGGC TTGGGTCTGA ATTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PIKFYVE ( XM_011510782.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011509084.1 Homo sapiens phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X6, mRNA.

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CloneID	OHu77664
Clone ID Related Accession (Same CDS sequence)	XM_011510782.2 , XM_011510782.3
Accession Version	XM_011510782.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6168bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	1-phosphatidylinositol 3-phosphate 5-kinase isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011510782.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG ATGATAAGAC GTCCCCAACA CTGGACTCTG CTAATGATTT GCCTCGATCT 
CCTACTAGTC CTTCTCATCT CACACACTTT AAACCTTTGA CTCCTGATCA AGATGAGCCC 
CCTTTTAAAT CAGCTTATAG TTCTTTTGTA AATCTCTTTC GTTTTAACAA AGAGAGAGCA 
GAAGGAGGCC AGGGAGAACA GCAGCCTTTG AGTGGAAGTT GGACCAGCCC TCAGCTCCCT 
TCGAGGACAC AGTCTGTTAG GTCACCCACA CCTTATAAAA AGCAGCTTAA TGAGGAACTC 
CAGCGGCGCT CTTCAGCATT AGCAGACAAC AGTTTGCAAC ATCCCCAGGA GAACACAGAC 
ACAAGAAGGA AAGCAGAACC TACCTTTGGA GGTCATGACC CTCGTACAGC TGTTCAGCTT 
CGAAGCCTCA GCACAGTATT AAAACGCCTC AAGGAAATCA TGGAGGGGAA AAGCCAGGAT 
AGTGACCTGA AACAATACTG GATGCCAGAT AGCCAATGTA AAGAGTGCTA TGACTGTAGT 
GAGAAATTTA CAACCTTTAG GCGCAGACAC CATTGCCGAC TATGTGGGCA GATTTTCTGC 
AGTCGTTGCT GTAATCAAGA AATCCCTGGA AAATTTATGG GCTATACAGG AGACCTCCGA 
GCTTGCACAT ATTGTAGAAA AATAGCCTTA AGTTATGCTC ATTCCACAGA CAGTAATTCT 
ATTGGGGAAG ACTTGAATGC TCTTTCAGAT TCTGCTTGCT CTGTGTCTGT GCTTGATCCA 
AGTGAACCCC GAACACCTGT TGGGAGTAGG AAAGCCAGCC GTAACATATT TTTAGAGGAT 
GATTTGGCCT GGCAAAGTTT GATTCATCCA GATTCCTCAA ATACTCCTCT TTCAACAAGA 
CTTGTATCTG TGCAAGAGGA TGCTGGGAAA TCTCCTGCTC GAAATAGATC AGCCAGCATT 
ACTAACCTGT CACTGGATAG ATCTGGTTCT CCTATGGTAC CTTCATATGA GACATCTGTC 
AGTCCCCAGG CTAACCGAAC ATATGTTAGG ACAGAGACCA CTGAGGATGA ACGCAAAATT 
CTTCTGGACA GTGTGCAGTT AAAAGACCTG TGGAAAAAAA TCTGCCATCA CAGCAGTGGA 
ATGGAGTTTC AGGATCACCG CTACTGGTTG AGAACGCATC CCAACTGCAT TGTAGGAAAG 
GAATTAGTCA ACTGGCTAAT CCGAAATGGG CATATTGCCA CAAGGGCACA AGCTATAGCA 
ATTGGACAAG CAATGGTTGA TGGACGTTGG CTGGATTGTG TTAGTCATCA CGACCAGCTT 
TTCAGAGATG AGTATGCGCT GTATAGACCA CTGCAGAGTA CAGAATTTTC TGAGACGCCT 
TCTCCCGACA GTGACTCAGT GAACTCCGTG GAAGGACACT CTGAGCCATC CTGGTTTAAA 
GACATAAAGT TTGATGACAG TGACACAGAA CAGATAGCTG AAGAAGGTGA CGATAATTTG 
GCTAATTCTG CCAGTCCTAG CAAGCGCACA TCAGTCAGCA GTTTCCAGTC CACAGTGGAC 
AGTGACTCAG CCGCTTCTAT CAGCCTGAAC GTGGAGCTGG ACAACGTGAA CTTCCATATC 
AAGAAGCCCT CCAAGTACCC ACATGTGCCC CCTCACCCTG CTGACCAAAA AGAGTATTTG 
ATTTCTGACA CTGGAGGACA ACAGCTCTCA ATAAGTGACG CTTTCATCAA AGAATCCTTA 
TTTAATCGCC GAGTAGAGGA AAAATCCAAA GAGCTGCCTT TCACACCTTT GGGCTGGCAT 
CATAACAACC TGGAGCTCCT GAGGGAGGAG AATGGGGAGA AACAAGCCAT GGAGAGGTTG 
CTTTCAGCTA ATCATAACCA CATGATGGCA CTACTCCAGC AGTTGCTCCA TAGTGACTCA 
CTGTCATCAT CTTGGAGGGA CATCATCGTG TCATTGGTCT GCCAGGTTGT TCAGACAGTC 
CGACCTGATG TCAAGAACCA GGATGATGAC ATGGATATCC GTCAGTTTGT CCACATCAAA 
AAAATCCCAG GTGGAAAGAA GTTTGATTCT GTGGTTGTCA ATGGCTTTGT TTGTACCAAG 
AACATTGCAC ATAAAAAGAT GAGTTCTTGT ATTAAAAACC CCAAAATTCT TCTGTTGAAG 
TGTTCCATTG AGTATCTCTA CAGAGAAGAA ACTAAGTTTA CTTGCATTGA TCCTATTGTG 
CTTCAGGAAA GGGAATTCTT GAAGAATTAT GTCCAGCGAA TAGTTGATGT TCGACCCACC 
TTGGTTCTTG TTGAGAAAAC AGTGTCTCGG ATTGCCCAGG ACATGTTATT GGAACATGGC 
ATTACTTTGG TCATTAATGT AAAGTCACAA GTTTTGGAAC GAATCAGTCG AATGACCCAA 
GGTGATTTAG TGATGTCAAT GGACCAGCTG CTTACGAAAC CACACCTGGG CACTTGTCAC 
AAATTTTATA TGCAGATATT TCAGTTGCCT AATGAACAAA CCAAGACACT GATGTTTTTT 
GAAGGTTGTC CACAGCACCT AGGCTGTACA ATCAAGCTAA GAGGAGGCTC TGATTATGAG 
CTGGCTCGAG TTAAGGAGAT CCTAATATTT ATGATCTGTG TTGCTTATCA TTCTCAACTA 
GAAATATCCT TTCTCATGGA TGAATTTGCT ATGCCTCCCA CATTAATGCA AAACCCTTCA 
TTCCATTCCC TGATTGAGGG ACGAGGGCAT GAGGGGGCTG TCCAAGAGCA GTACGGTGGA 
GGTTCCATCC CCTGGGATCC TGACATCCCT CCTGAGTCTC TGCCCTGTGA TGATAGCAGT 
TTGCTGGAAT TGAGGATTGT GTTTGAGAAG GGTGAGCAGG AAAATAAAAA TCTTCCGCAG 
GCTGTTGCCT CTGTGAAGCA TCAAGAACAT AGCACAACAG CTTGCCCGGC GGGTCTCCCT 
TGTGCTTTCT TTGCACCTGT ACCGGAATCA TTGTTGCCAC TCCCTGTGGA TGACCAACAA 
GATGCTTTAG GCAGCGAGCA GCCAGAGACT TTGCAGCAAA CAGTTGTGCT GCAGGATCCC 
AAAAGCCAGA TAAGAGCCTT TAGAGACCCT CTACAGGATG ACACTGGATT ATATGTTACT 
GAGGAAGTCA CCTCCTCTGA AGATAAACGA AAGACTTATT CTTTGGCCTT TAAGCAGGAA 
TTAAAAGATG TGATCCTCTG TATCTCCCCA GTAATCACAT TCCGAGAACC CTTTCTTTTA 
ACTGAAAAGG GGATGAGATG CTCTACCCGA GATTATTTTG CAGAGCAGGT TTACTGGTCT 
CCTCTCCTCA ATAAAGAATT CAAAGAAATG GAGAACAGGA GGAAGAAACA GCTGCTCAGG 
GATCTCTCTG GACTTCAGGG CATGAATGGA AGTATTCAGG CCAAGTCTAT TCAAGTCTTA 
CCCTCACATG AGCTAGTGAG CACTAGAATT GCTGAGCATC TGGGCGATAG CCAGAGCTTG 
GGTAGAATGC TGGCCGATTA TCGAGCCAGA GGAGGAAGAA TTCAGCCCAA AAATTCAGAC 
CCTTTTGCTC ATTCAAAGGA TGCATCAAGT ACTTCAAGTG GCCAATCAGG AAGCAAAAAT 
GAGGGTGATG AAGAGAGAGG GCTTATTCTG AGTGATGCTG TGTGGTCAAC AAAGGTGGAC 
TGTCTGAATC CCATTAATCA CCAGAGACTT TGTGTGCTCT TCAGCAGCTC TTCTGCCCAG 
TCCAGCAATG CTCCTAGTGC CTGTGTCAGT CCTTGGATTG TAACAATGGA ATTTTATGGA 
AAGAATGATC TTACATTAGG AATATTTTTA GAGAGATACT GTTTCAGGCC TTCTTATCAG 
TGTCCAAGCA TGTTCTGTGA TACCCCCATG GTACATCATA TTCGGCGCTT TGTTCATGGC 
CAAGGCTGTG TGCAGATAAT CCTGAAGGAG TTGGATTCTC CAGTACCTGG ATATCAGCAT 
ACAATTCTTA CATATTCCTG GTGTAGAATC TGCAAACAGG TAACACCAGT TGTTGCTCTT 
TCCAATGAGT CCTGGTCTAT GTCATTTGCA AAATACCTTG AACTTAGGTT TTATGGGCAC 
CAGTATACTC GCAGAGCCAA CGCTGAGCCC TGTGGTCACT CCATCCATCA TGATTATCAC 
CAGTATTTCT CCTATAACCA GATGGTGGCG TCTTTCAGTT ATTCTCCCAT TCGGCTTCTT 
GAAGTATGTG TTCCACTCCC CAAAATATTC ATTAAGCGTC AGGCCCCATT AAAAGTGTCC 
CTTCTTCAGG ATCTGAAGGA CTTCTTTCAA AAAGTTTCAC AGGTATATGT TGCCATTGAT 
GAAAGACTTG CATCTTTGAA AACTGATACA TTTAGTAAAA CAAGAGAGGA AAAAATGGAA 
GATATTTTTG CACAGAAAGA GATGGAAGAA GGTGAGTTCA AGAACTGGAT TGAGAAGATG 
CAAGCAAGGC TCATGTCTTC CTCTGTAGAT ACCCCTCAGC AACTGCAGTC GGTCTTTGAG 
TCACTCATTG CCAAGAAACA AAGTCTCTGT GAAGTGCTGC AAGCTTGGAA TAACAGGTTG 
CAGGACCTTT TCCAACAGGA AAAGGGTAGA AAGAGACCTT CAGTTCCTCC AAGTCCTGGA 
AGACTGAGAC AAGGGGAAGA AAGCAAGATA AGTGCGATGG ATGCATCTCC ACGGAATATT 
TCTCCAGGAC TTCAGAATGG AGAAAAAGAG GATCGCTTCT TAACAACTTT GTCCAGCCAG 
AGCTCCACCA GTTCTACTCA TCTCCAATTG CCTACGCCAC CTGAAGTCAT GTCTGAACAG 
TCAGTGGGAG GGCCCCCTGA GCTAGATACA GCCAGCAGTT CCGAAGATGT GTTTGATGGG 
CATTTGCTGG GATCCACAGA CAGCCAAGTG AAGGAAAAGT CAACCATGAA AGCCATCTTT 
GCAAATTTGC TTCCAGGAAA TAGCTATAAT CCTATTCCAT TTCCTTTTGA TCCAGATAAA 
CACTACTTAA TGTATGAACA TGAACGAGTG CCCATTGCAG TCTGCGAGAA GGAACCCAGC 
TCCATCATTG CTTTTGCTCT CAGTTGTAAA GAATACCGAA ATGCCTTAGA GGAATTGTCT 
AAAGCGACTC AGTGGAACAG TGCCGAAGAA GGGCTTCCAA CAAATAGTAC TTCAGATAGC 
AGACCAAAGA GTAGCAGCCC TATCAGATTA CCTGAAATGA GTGGAGGACA GACAAATCGT 
ACAACAGAAA CAGAACCACA ACCAAATGAA GTAGATGGAG GAGATACACA AAAGAAGCAA 
CTCATAAATC CTCATGTGGA ACTTCAATTT TCAGATGCTA ATGCCAAGTT TTACTGTCGG 
CTCTACTATG CGGGAGAGTT TCATAAGATG CGTGAAGTGA TTCTGGACAG CAGTGAAGAA 
GATTTCATTC GTTCCCTCTC CCACTCATCA CCCTGGCAGG CCCGGGGAGG CAAATCAGGA 
GCTGCCTTCT ATGCAACTGA GGATGATAGA TTTATTTTGA AGCAAATGCC TCGTCTGGAA 
GTCCAGTCCT TCCTCGACTT TGCACCACAT TACTTCAATT ATATTACAAA TGCTGTTCAA 
CAAAAGAGGC CCACGGCGTT GGCCAAAATT CTTGGAGTTT ACAGAATTGG TTATAAGAAC 
TCTCAGAACA ACACTGAGAA GAAGTTAGAT CTCCTTGTCA TGGAAAATCT TTTCTACGGG 
AGAAAGATGG CACAGGTTTT TGATTTGAAG GGCTCTCTTA GGAATCGGAA TGTAAAAACT 
GACACTGGAA AAGAGAGTTG TGATGTGGTC CTGCTAGATG AAAATCTCCT AAAGATGGTT 
CGAGACAACC CTCTATATAT TCGTTCTCAT TCCAAAGCTG TGCTGAGAAC CTCGATCCAT 
AGTGACTCCC ATTTCCTTTC TAGCCACCTC ATTATAGATT ATTCTTTGCT GGTTGGGCGA 
GATGATACTA GCAATGAGCT AGTAGTTGGA ATTATAGATT ATATTCGAAC ATTTACATGG 
GACAAAAAGC TTGAGATGGT TGTGAAATCA ACAGGAATTT TAGGTGGACA AGGTAAAATG 
CCAACAGTGG TGTCTCCGGA GTTGTACAGG ACTAGGTTTT GTGAGGCAAT GGACAAGTAT 
TTCCTAATGG TACCAGACCA CTGGACAGGC TTGGGTCTGA ATTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011509084.1
CDS	182..6349
Translation	MATDDKTSPTLDSANDLPRSPTSPSHLTHFKPLTPDQDEPPFKSAYSSFVNLFRFNKERAEGGQGEQQPLSGSWTSPQLPSRTQSVRSPTPYKKQLNEELQRRSSALADNSLQHPQENTDTRRKAEPTFGGHDPRTAVQLRSLSTVLKRLKEIMEGKSQDSDLKQYWMPDSQCKECYDCSEKFTTFRRRHHCRLCGQIFCSRCCNQEIPGKFMGYTGDLRACTYCRKIALSYAHSTDSNSIGEDLNALSDSACSVSVLDPSEPRTPVGSRKASRNIFLEDDLAWQSLIHPDSSNTPLSTRLVSVQEDAGKSPARNRSASITNLSLDRSGSPMVPSYETSVSPQANRTYVRTETTEDERKILLDSVQLKDLWKKICHHSSGMEFQDHRYWLRTHPNCIVGKELVNWLIRNGHIATRAQAIAIGQAMVDGRWLDCVSHHDQLFRDEYALYRPLQSTEFSETPSPDSDSVNSVEGHSEPSWFKDIKFDDSDTEQIAEEGDDNLANSASPSKRTSVSSFQSTVDSDSAASISLNVELDNVNFHIKKPSKYPHVPPHPADQKEYLISDTGGQQLSISDAFIKESLFNRRVEEKSKELPFTPLGWHHNNLELLREENGEKQAMERLLSANHNHMMALLQQLLHSDSLSSSWRDIIVSLVCQVVQTVRPDVKNQDDDMDIRQFVHIKKIPGGKKFDSVVVNGFVCTKNIAHKKMSSCIKNPKILLLKCSIEYLYREETKFTCIDPIVLQEREFLKNYVQRIVDVRPTLVLVEKTVSRIAQDMLLEHGITLVINVKSQVLERISRMTQGDLVMSMDQLLTKPHLGTCHKFYMQIFQLPNEQTKTLMFFEGCPQHLGCTIKLRGGSDYELARVKEILIFMICVAYHSQLEISFLMDEFAMPPTLMQNPSFHSLIEGRGHEGAVQEQYGGGSIPWDPDIPPESLPCDDSSLLELRIVFEKGEQENKNLPQAVASVKHQEHSTTACPAGLPCAFFAPVPESLLPLPVDDQQDALGSEQPETLQQTVVLQDPKSQIRAFRDPLQDDTGLYVTEEVTSSEDKRKTYSLAFKQELKDVILCISPVITFREPFLLTEKGMRCSTRDYFAEQVYWSPLLNKEFKEMENRRKKQLLRDLSGLQGMNGSIQAKSIQVLPSHELVSTRIAEHLGDSQSLGRMLADYRARGGRIQPKNSDPFAHSKDASSTSSGQSGSKNEGDEERGLILSDAVWSTKVDCLNPINHQRLCVLFSSSSAQSSNAPSACVSPWIVTMEFYGKNDLTLGIFLERYCFRPSYQCPSMFCDTPMVHHIRRFVHGQGCVQIILKELDSPVPGYQHTILTYSWCRICKQVTPVVALSNESWSMSFAKYLELRFYGHQYTRRANAEPCGHSIHHDYHQYFSYNQMVASFSYSPIRLLEVCVPLPKIFIKRQAPLKVSLLQDLKDFFQKVSQVYVAIDERLASLKTDTFSKTREEKMEDIFAQKEMEEGEFKNWIEKMQARLMSSSVDTPQQLQSVFESLIAKKQSLCEVLQAWNNRLQDLFQQEKGRKRPSVPPSPGRLRQGEESKISAMDASPRNISPGLQNGEKEDRFLTTLSSQSSTSSTHLQLPTPPEVMSEQSVGGPPELDTASSSEDVFDGHLLGSTDSQVKEKSTMKAIFANLLPGNSYNPIPFPFDPDKHYLMYEHERVPIAVCEKEPSSIIAFALSCKEYRNALEELSKATQWNSAEEGLPTNSTSDSRPKSSSPIRLPEMSGGQTNRTTETEPQPNEVDGGDTQKKQLINPHVELQFSDANAKFYCRLYYAGEFHKMREVILDSSEEDFIRSLSHSSPWQARGGKSGAAFYATEDDRFILKQMPRLEVQSFLDFAPHYFNYITNAVQQKRPTALAKILGVYRIGYKNSQNNTEKKLDLLVMENLFYGRKMAQVFDLKGSLRNRNVKTDTGKESCDVVLLDENLLKMVRDNPLYIRSHSKAVLRTSIHSDSHFLSSHLIIDYSLLVGRDDTSNELVVGIIDYIRTFTWDKKLEMVVKSTGILGGQGKMPTVVSPELYRTRFCEAMDKYFLMVPDHWTGLGLNC

Target ORF information:

RefSeq Version	XM_011510782.3
Organism	Homo sapiens(human)
Definition	Homo sapiens phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011510782.3

ORF Insert Sequence:

ATGGCCACAG ATGATAAGAC GTCCCCAACA CTGGACTCTG CTAATGATTT GCCTCGATCT 
CCTACTAGTC CTTCTCATCT CACACACTTT AAACCTTTGA CTCCTGATCA AGATGAGCCC 
CCTTTTAAAT CAGCTTATAG TTCTTTTGTA AATCTCTTTC GTTTTAACAA AGAGAGAGCA 
GAAGGAGGCC AGGGAGAACA GCAGCCTTTG AGTGGAAGTT GGACCAGCCC TCAGCTCCCT 
TCGAGGACAC AGTCTGTTAG GTCACCCACA CCTTATAAAA AGCAGCTTAA TGAGGAACTC 
CAGCGGCGCT CTTCAGCATT AGCAGACAAC AGTTTGCAAC ATCCCCAGGA GAACACAGAC 
ACAAGAAGGA AAGCAGAACC TACCTTTGGA GGTCATGACC CTCGTACAGC TGTTCAGCTT 
CGAAGCCTCA GCACAGTATT AAAACGCCTC AAGGAAATCA TGGAGGGGAA AAGCCAGGAT 
AGTGACCTGA AACAATACTG GATGCCAGAT AGCCAATGTA AAGAGTGCTA TGACTGTAGT 
GAGAAATTTA CAACCTTTAG GCGCAGACAC CATTGCCGAC TATGTGGGCA GATTTTCTGC 
AGTCGTTGCT GTAATCAAGA AATCCCTGGA AAATTTATGG GCTATACAGG AGACCTCCGA 
GCTTGCACAT ATTGTAGAAA AATAGCCTTA AGTTATGCTC ATTCCACAGA CAGTAATTCT 
ATTGGGGAAG ACTTGAATGC TCTTTCAGAT TCTGCTTGCT CTGTGTCTGT GCTTGATCCA 
AGTGAACCCC GAACACCTGT TGGGAGTAGG AAAGCCAGCC GTAACATATT TTTAGAGGAT 
GATTTGGCCT GGCAAAGTTT GATTCATCCA GATTCCTCAA ATACTCCTCT TTCAACAAGA 
CTTGTATCTG TGCAAGAGGA TGCTGGGAAA TCTCCTGCTC GAAATAGATC AGCCAGCATT 
ACTAACCTGT CACTGGATAG ATCTGGTTCT CCTATGGTAC CTTCATATGA GACATCTGTC 
AGTCCCCAGG CTAACCGAAC ATATGTTAGG ACAGAGACCA CTGAGGATGA ACGCAAAATT 
CTTCTGGACA GTGTGCAGTT AAAAGACCTG TGGAAAAAAA TCTGCCATCA CAGCAGTGGA 
ATGGAGTTTC AGGATCACCG CTACTGGTTG AGAACGCATC CCAACTGCAT TGTAGGAAAG 
GAATTAGTCA ACTGGCTAAT CCGAAATGGG CATATTGCCA CAAGGGCACA AGCTATAGCA 
ATTGGACAAG CAATGGTTGA TGGACGTTGG CTGGATTGTG TTAGTCATCA CGACCAGCTT 
TTCAGAGATG AGTATGCGCT GTATAGACCA CTGCAGAGTA CAGAATTTTC TGAGACGCCT 
TCTCCCGACA GTGACTCAGT GAACTCCGTG GAAGGACACT CTGAGCCATC CTGGTTTAAA 
GACATAAAGT TTGATGACAG TGACACAGAA CAGATAGCTG AAGAAGGTGA CGATAATTTG 
GCTAATTCTG CCAGTCCTAG CAAGCGCACA TCAGTCAGCA GTTTCCAGTC CACAGTGGAC 
AGTGACTCAG CCGCTTCTAT CAGCCTGAAC GTGGAGCTGG ACAACGTGAA CTTCCATATC 
AAGAAGCCCT CCAAGTACCC ACATGTGCCC CCTCACCCTG CTGACCAAAA AGAGTATTTG 
ATTTCTGACA CTGGAGGACA ACAGCTCTCA ATAAGTGACG CTTTCATCAA AGAATCCTTA 
TTTAATCGCC GAGTAGAGGA AAAATCCAAA GAGCTGCCTT TCACACCTTT GGGCTGGCAT 
CATAACAACC TGGAGCTCCT GAGGGAGGAG AATGGGGAGA AACAAGCCAT GGAGAGGTTG 
CTTTCAGCTA ATCATAACCA CATGATGGCA CTACTCCAGC AGTTGCTCCA TAGTGACTCA 
CTGTCATCAT CTTGGAGGGA CATCATCGTG TCATTGGTCT GCCAGGTTGT TCAGACAGTC 
CGACCTGATG TCAAGAACCA GGATGATGAC ATGGATATCC GTCAGTTTGT CCACATCAAA 
AAAATCCCAG GTGGAAAGAA GTTTGATTCT GTGGTTGTCA ATGGCTTTGT TTGTACCAAG 
AACATTGCAC ATAAAAAGAT GAGTTCTTGT ATTAAAAACC CCAAAATTCT TCTGTTGAAG 
TGTTCCATTG AGTATCTCTA CAGAGAAGAA ACTAAGTTTA CTTGCATTGA TCCTATTGTG 
CTTCAGGAAA GGGAATTCTT GAAGAATTAT GTCCAGCGAA TAGTTGATGT TCGACCCACC 
TTGGTTCTTG TTGAGAAAAC AGTGTCTCGG ATTGCCCAGG ACATGTTATT GGAACATGGC 
ATTACTTTGG TCATTAATGT AAAGTCACAA GTTTTGGAAC GAATCAGTCG AATGACCCAA 
GGTGATTTAG TGATGTCAAT GGACCAGCTG CTTACGAAAC CACACCTGGG CACTTGTCAC 
AAATTTTATA TGCAGATATT TCAGTTGCCT AATGAACAAA CCAAGACACT GATGTTTTTT 
GAAGGTTGTC CACAGCACCT AGGCTGTACA ATCAAGCTAA GAGGAGGCTC TGATTATGAG 
CTGGCTCGAG TTAAGGAGAT CCTAATATTT ATGATCTGTG TTGCTTATCA TTCTCAACTA 
GAAATATCCT TTCTCATGGA TGAATTTGCT ATGCCTCCCA CATTAATGCA AAACCCTTCA 
TTCCATTCCC TGATTGAGGG ACGAGGGCAT GAGGGGGCTG TCCAAGAGCA GTACGGTGGA 
GGTTCCATCC CCTGGGATCC TGACATCCCT CCTGAGTCTC TGCCCTGTGA TGATAGCAGT 
TTGCTGGAAT TGAGGATTGT GTTTGAGAAG GGTGAGCAGG AAAATAAAAA TCTTCCGCAG 
GCTGTTGCCT CTGTGAAGCA TCAAGAACAT AGCACAACAG CTTGCCCGGC GGGTCTCCCT 
TGTGCTTTCT TTGCACCTGT ACCGGAATCA TTGTTGCCAC TCCCTGTGGA TGACCAACAA 
GATGCTTTAG GCAGCGAGCA GCCAGAGACT TTGCAGCAAA CAGTTGTGCT GCAGGATCCC 
AAAAGCCAGA TAAGAGCCTT TAGAGACCCT CTACAGGATG ACACTGGATT ATATGTTACT 
GAGGAAGTCA CCTCCTCTGA AGATAAACGA AAGACTTATT CTTTGGCCTT TAAGCAGGAA 
TTAAAAGATG TGATCCTCTG TATCTCCCCA GTAATCACAT TCCGAGAACC CTTTCTTTTA 
ACTGAAAAGG GGATGAGATG CTCTACCCGA GATTATTTTG CAGAGCAGGT TTACTGGTCT 
CCTCTCCTCA ATAAAGAATT CAAAGAAATG GAGAACAGGA GGAAGAAACA GCTGCTCAGG 
GATCTCTCTG GACTTCAGGG CATGAATGGA AGTATTCAGG CCAAGTCTAT TCAAGTCTTA 
CCCTCACATG AGCTAGTGAG CACTAGAATT GCTGAGCATC TGGGCGATAG CCAGAGCTTG 
GGTAGAATGC TGGCCGATTA TCGAGCCAGA GGAGGAAGAA TTCAGCCCAA AAATTCAGAC 
CCTTTTGCTC ATTCAAAGGA TGCATCAAGT ACTTCAAGTG GCCAATCAGG AAGCAAAAAT 
GAGGGTGATG AAGAGAGAGG GCTTATTCTG AGTGATGCTG TGTGGTCAAC AAAGGTGGAC 
TGTCTGAATC CCATTAATCA CCAGAGACTT TGTGTGCTCT TCAGCAGCTC TTCTGCCCAG 
TCCAGCAATG CTCCTAGTGC CTGTGTCAGT CCTTGGATTG TAACAATGGA ATTTTATGGA 
AAGAATGATC TTACATTAGG AATATTTTTA GAGAGATACT GTTTCAGGCC TTCTTATCAG 
TGTCCAAGCA TGTTCTGTGA TACCCCCATG GTACATCATA TTCGGCGCTT TGTTCATGGC 
CAAGGCTGTG TGCAGATAAT CCTGAAGGAG TTGGATTCTC CAGTACCTGG ATATCAGCAT 
ACAATTCTTA CATATTCCTG GTGTAGAATC TGCAAACAGG TAACACCAGT TGTTGCTCTT 
TCCAATGAGT CCTGGTCTAT GTCATTTGCA AAATACCTTG AACTTAGGTT TTATGGGCAC 
CAGTATACTC GCAGAGCCAA CGCTGAGCCC TGTGGTCACT CCATCCATCA TGATTATCAC 
CAGTATTTCT CCTATAACCA GATGGTGGCG TCTTTCAGTT ATTCTCCCAT TCGGCTTCTT 
GAAGTATGTG TTCCACTCCC CAAAATATTC ATTAAGCGTC AGGCCCCATT AAAAGTGTCC 
CTTCTTCAGG ATCTGAAGGA CTTCTTTCAA AAAGTTTCAC AGGTATATGT TGCCATTGAT 
GAAAGACTTG CATCTTTGAA AACTGATACA TTTAGTAAAA CAAGAGAGGA AAAAATGGAA 
GATATTTTTG CACAGAAAGA GATGGAAGAA GGTGAGTTCA AGAACTGGAT TGAGAAGATG 
CAAGCAAGGC TCATGTCTTC CTCTGTAGAT ACCCCTCAGC AACTGCAGTC GGTCTTTGAG 
TCACTCATTG CCAAGAAACA AAGTCTCTGT GAAGTGCTGC AAGCTTGGAA TAACAGGTTG 
CAGGACCTTT TCCAACAGGA AAAGGGTAGA AAGAGACCTT CAGTTCCTCC AAGTCCTGGA 
AGACTGAGAC AAGGGGAAGA AAGCAAGATA AGTGCGATGG ATGCATCTCC ACGGAATATT 
TCTCCAGGAC TTCAGAATGG AGAAAAAGAG GATCGCTTCT TAACAACTTT GTCCAGCCAG 
AGCTCCACCA GTTCTACTCA TCTCCAATTG CCTACGCCAC CTGAAGTCAT GTCTGAACAG 
TCAGTGGGAG GGCCCCCTGA GCTAGATACA GCCAGCAGTT CCGAAGATGT GTTTGATGGG 
CATTTGCTGG GATCCACAGA CAGCCAAGTG AAGGAAAAGT CAACCATGAA AGCCATCTTT 
GCAAATTTGC TTCCAGGAAA TAGCTATAAT CCTATTCCAT TTCCTTTTGA TCCAGATAAA 
CACTACTTAA TGTATGAACA TGAACGAGTG CCCATTGCAG TCTGCGAGAA GGAACCCAGC 
TCCATCATTG CTTTTGCTCT CAGTTGTAAA GAATACCGAA ATGCCTTAGA GGAATTGTCT 
AAAGCGACTC AGTGGAACAG TGCCGAAGAA GGGCTTCCAA CAAATAGTAC TTCAGATAGC 
AGACCAAAGA GTAGCAGCCC TATCAGATTA CCTGAAATGA GTGGAGGACA GACAAATCGT 
ACAACAGAAA CAGAACCACA ACCAAATGAA GTAGATGGAG GAGATACACA AAAGAAGCAA 
CTCATAAATC CTCATGTGGA ACTTCAATTT TCAGATGCTA ATGCCAAGTT TTACTGTCGG 
CTCTACTATG CGGGAGAGTT TCATAAGATG CGTGAAGTGA TTCTGGACAG CAGTGAAGAA 
GATTTCATTC GTTCCCTCTC CCACTCATCA CCCTGGCAGG CCCGGGGAGG CAAATCAGGA 
GCTGCCTTCT ATGCAACTGA GGATGATAGA TTTATTTTGA AGCAAATGCC TCGTCTGGAA 
GTCCAGTCCT TCCTCGACTT TGCACCACAT TACTTCAATT ATATTACAAA TGCTGTTCAA 
CAAAAGAGGC CCACGGCGTT GGCCAAAATT CTTGGAGTTT ACAGAATTGG TTATAAGAAC 
TCTCAGAACA ACACTGAGAA GAAGTTAGAT CTCCTTGTCA TGGAAAATCT TTTCTACGGG 
AGAAAGATGG CACAGGTTTT TGATTTGAAG GGCTCTCTTA GGAATCGGAA TGTAAAAACT 
GACACTGGAA AAGAGAGTTG TGATGTGGTC CTGCTAGATG AAAATCTCCT AAAGATGGTT 
CGAGACAACC CTCTATATAT TCGTTCTCAT TCCAAAGCTG TGCTGAGAAC CTCGATCCAT 
AGTGACTCCC ATTTCCTTTC TAGCCACCTC ATTATAGATT ATTCTTTGCT GGTTGGGCGA 
GATGATACTA GCAATGAGCT AGTAGTTGGA ATTATAGATT ATATTCGAAC ATTTACATGG 
GACAAAAAGC TTGAGATGGT TGTGAAATCA ACAGGAATTT TAGGTGGACA AGGTAAAATG 
CCAACAGTGG TGTCTCCGGA GTTGTACAGG ACTAGGTTTT GTGAGGCAAT GGACAAGTAT 
TTCCTAATGG TACCAGACCA CTGGACAGGC TTGGGTCTGA ATTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PIKFYVE ( XM_011510783.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011509085.1 Homo sapiens phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X7, mRNA.

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CloneID	OHu77665
Clone ID Related Accession (Same CDS sequence)	XM_011510783.2 , XM_011510783.3
Accession Version	XM_011510783.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6165bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	1-phosphatidylinositol 3-phosphate 5-kinase isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011510783.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG ATGATAAGAC GTCCCCAACA CTGGACTCTG CTAATGATTT GCCTCGATCT 
CCTACTAGTC CTTCTCATCT CACACACTTT AAACCTTTGA CTCCTGATCA AGATGAGCCC 
CCTTTTAAAT CAGCTTATAG TTCTTTTGTA AATCTCTTTC GTTTTAACAA AGAGAGAGCA 
GAAGGAGGCC AGGGAGAACA GCAGCCTTTG AGTGGAAGTT GGACCAGCCC TCAGCTCCCT 
TCGAGGACAC AGTCTGTTAG GTCACCCACA CCTTATAAAA AGCAGCTTAA TGAGGAACTC 
CAGCGGCGCT CTTCAGCATT AGCAGACAAC AGTTTGCAAC ATCCCCAGGA GAACACAGAC 
ACAAGAAGGA AAGCAGAACC TACCTTTGGA GGTCATGACC CTCGTACAGC TGTTCAGCTT 
CGAAGCCTCA GCACAGTATT AAAACGCCTC AAGGAAATCA TGGAGGGGAA AAGCCAGGAT 
AGTGACCTGA AACAATACTG GATGCCAGAT AGCCAATGTA AAGAGTGCTA TGACTGTAGT 
GAGAAATTTA CAACCTTTAG GCGCAGACAC CATTGCCGAC TATGTGGGCA GATTTTCTGC 
AGTCGTTGCT GTAATCAAGA AATCCCTGGA AAATTTATGG GCTATACAGG AGACCTCCGA 
GCTTGCACAT ATTGTAGAAA AATAGCCTTA AGTTATGCTC ATTCCACAGA CAGTAATTCT 
ATTGGGGAAG ACTTGAATGC TCTTTCAGAT TCTGCTTGCT CTGTGTCTGT GCTTGATCCA 
AGTGAACCCC GAACACCTGT TGGGAGTAGG AAAGCCAGCC GTAACATATT TTTAGAGGAT 
GATTTGGCCT GGCAAAGTTT GATTCATCCA GATTCCTCAA ATACTCCTCT TTCAACAAGA 
CTTGTATCTG TGCAAGAGGA TGCTGGGAAA TCTCCTGCTC GAAATAGATC AGCCAGCATT 
ACTAACCTGT CACTGGATAG ATCTGGTTCT CCTATGGTAC CTTCATATGA GACATCTGTC 
AGTCCCCAGG CTAACCGAAC ATATGTTAGG ACAGAGACCA CTGAGGATGA ACGCAAAATT 
CTTCTGGACA GTGTGCAGTT AAAAGACCTG TGGAAAAAAA TCTGCCATCA CAGCAGTGGA 
ATGGAGTTTC AGGATCACCG CTACTGGTTG AGAACGCATC CCAACTGCAT TGTAGGAAAG 
GAATTAGTCA ACTGGCTAAT CCGAAATGGG CATATTGCCA CAAGGGCACA AGCTATAGCA 
ATTGGACAAG CAATGGTTGA TGGACGTTGG CTGGATTGTG TTAGTCATCA CGACCAGCTT 
TTCAGAGATG AGTATGCGCT GTATAGACCA CTGCAGAGTA CAGAATTTTC TGAGACGCCT 
TCTCCCGACA GTGACTCAGT GAACTCCGTG GAAGGACACT CTGAGCCATC CTGGTTTAAA 
GACATAAAGT TTGATGACAG TGACACAGAA CAGATAGCTG AAGAAGGTGA CGATAATTTG 
GCTAAGTATT TGATTTCTGA CACTGGAGGA CAACAGCTCT CAATAAGTGA CGCTTTCATC 
AAAGAATCCT TATTTAATCG CCGAGTAGAG GAAAAATCCA AAGAGCTGCC TTTCACACCT 
TTGGGCTGGC ATCATAACAA CCTGGAGCTC CTGAGGGAGG AGAATGGGGA GAAACAAGCC 
ATGGAGAGGT TGCTTTCAGC TAATCATAAC CACATGATGG CACTACTCCA GCAGTTGCTC 
CATAGTGACT CACTGTCATC ATCTTGGAGG GACATCATCG TGTCATTGGT CTGCCAGGTT 
GTTCAGACAG TCCGACCTGA TGTCAAGAAC CAGGATGATG ACATGGATAT CCGTCAGTTT 
GTCCACATCA AAAAAATCCC AGGTGGAAAG AAGTTTGATT CTGTGGTTGT CAATGGCTTT 
GTTTGTACCA AGAACATTGC ACATAAAAAG ATGAGTTCTT GTATTAAAAA CCCCAAAATT 
CTTCTGTTGA AGTGTTCCAT TGAGTATCTC TACAGAGAAG AAACTAAGTT TACTTGCATT 
GATCCTATTG TGCTTCAGGA AAGGGAATTC TTGAAGAATT ATGTCCAGCG AATAGTTGAT 
GTTCGACCCA CCTTGGTTCT TGTTGAGAAA ACAGTGTCTC GGATTGCCCA GGACATGTTA 
TTGGAACATG GCATTACTTT GGTCATTAAT GTAAAGTCAC AAGTTTTGGA ACGAATCAGT 
CGAATGACCC AAGGTGATTT AGTGATGTCA ATGGACCAGC TGCTTACGAA ACCACACCTG 
GGCACTTGTC ACAAATTTTA TATGCAGATA TTTCAGTTGC CTAATGAACA AACCAAGACA 
CTGATGTTTT TTGAAGGTTG TCCACAGCAC CTAGGCTGTA CAATCAAGCT AAGAGGAGGC 
TCTGATTATG AGCTGGCTCG AGTTAAGGAG ATCCTAATAT TTATGATCTG TGTTGCTTAT 
CATTCTCAAC TAGAAATATC CTTTCTCATG GATGAATTTG CTATGCCTCC CACATTAATG 
CAAAACCCTT CATTCCATTC CCTGATTGAG GGACGAGGGC ATGAGGGGGC TGTCCAAGAG 
CAGTACGGTG GAGGTTCCAT CCCCTGGGAT CCTGACATCC CTCCTGAGTC TCTGCCCTGT 
GATGATAGCA GTTTGCTGGA ATTGAGGATT GTGTTTGAGA AGGGTGAGCA GGAAAATAAA 
AATCTTCCGC AGGCTGTTGC CTCTGTGAAG CATCAAGAAC ATAGCACAAC AGCTTGCCCG 
GCGGGTCTCC CTTGTGCTTT CTTTGCACCT GTACCGGAAT CATTGTTGCC ACTCCCTGTG 
GATGACCAAC AAGATGCTTT AGGCAGCGAG CAGCCAGAGA CTTTGCAGCA AACAGTTGTG 
CTGCAGGATC CCAAAAGCCA GATAAGAGCC TTTAGAGACC CTCTACAGGA TGACACTGGA 
TTATATGTTA CTGAGGAAGT CACCTCCTCT GAAGATAAAC GAAAGACTTA TTCTTTGGCC 
TTTAAGCAGG AATTAAAAGA TGTGATCCTC TGTATCTCCC CAGTAATCAC ATTCCGAGAA 
CCCTTTCTTT TAACTGAAAA GGGGATGAGA TGCTCTACCC GAGATTATTT TGCAGAGCAG 
GTTTACTGGT CTCCTCTCCT CAATAAAGAA TTCAAAGAAA TGGAGAACAG GAGGAAGAAA 
CAGCTGCTCA GGGATCTCTC TGGACTTCAG GGCATGAATG GAAGTATTCA GGCCAAGTCT 
ATTCAAGTCT TACCCTCACA TGAGCTAGTG AGCACTAGAA TTGCTGAGCA TCTGGGCGAT 
AGCCAGAGCT TGGGTAGAAT GCTGGCCGAT TATCGAGCCA GAGGAGGAAG AATTCAGCCC 
AAAAATTCAG ACCCTTTTGC TCATTCAAAG GATGCATCAA GTACTTCAAG TGGCCAATCA 
GGAAGCAAAA ATGAGGGTGA TGAAGAGAGA GGGCTTATTC TGAGTGATGC TGTGTGGTCA 
ACAAAGGTGG ACTGTCTGAA TCCCATTAAT CACCAGAGAC TTTGTGTGCT CTTCAGCAGC 
TCTTCTGCCC AGTCCAGCAA TGCTCCTAGT GCCTGTGTCA GTCCTTGGAT TGTAACAATG 
GAATTTTATG GAAAGAATGA TCTTACATTA GGAATATTTT TAGAGAGATA CTGTTTCAGG 
CCTTCTTATC AGTGTCCAAG CATGTTCTGT GATACCCCCA TGGTACATCA TATTCGGCGC 
TTTGTTCATG GCCAAGGCTG TGTGCAGATA ATCCTGAAGG AGTTGGATTC TCCAGTACCT 
GGATATCAGC ATACAATTCT TACATATTCC TGGTGTAGAA TCTGCAAACA GGTAACACCA 
GTTGTTGCTC TTTCCAATGA GTCCTGGTCT ATGTCATTTG CAAAATACCT TGAACTTAGG 
TTTTATGGGC ACCAGTATAC TCGCAGAGCC AACGCTGAGC CCTGTGGTCA CTCCATCCAT 
CATGATTATC ACCAGTATTT CTCCTATAAC CAGATGGTGG CGTCTTTCAG TTATTCTCCC 
ATTCGGCTTC TTGAAGTATG TGTTCCACTC CCCAAAATAT TCATTAAGCG TCAGGCCCCA 
TTAAAAGTGT CCCTTCTTCA GGATCTGAAG GACTTCTTTC AAAAAGTTTC ACAGGTATAT 
GTTGCCATTG ATGAAAGACT TGCATCTTTG AAAACTGATA CATTTAGTAA AACAAGAGAG 
GAAAAAATGG AAGATATTTT TGCACAGAAA GAGATGGAAG AAGGTGAGTT CAAGAACTGG 
ATTGAGAAGA TGCAAGCAAG GCTCATGTCT TCCTCTGTAG ATACCCCTCA GCAACTGCAG 
TCGGTCTTTG AGTCACTCAT TGCCAAGAAA CAAAGTCTCT GTGAAGTGCT GCAAGCTTGG 
AATAACAGGT TGCAGGACCT TTTCCAACAG GAAAAGGGTA GAAAGAGACC TTCAGTTCCT 
CCAAGTCCTG GAAGACTGAG ACAAGGGGAA GAAAGCAAGA TAAGTGCGAT GGATGCATCT 
CCACGGAATA TTTCTCCAGG ACTTCAGAAT GGAGAAAAAG AGGATCGCTT CTTAACAACT 
TTGTCCAGCC AGAGCTCCAC CAGTTCTACT CATCTCCAAT TGCCTACGCC ACCTGAAGTC 
ATGTCTGAAC AGTCAGTGGG AGGGCCCCCT GAGCTAGATA CAGCCAGCAG TTCCGAAGAT 
GTGTTTGATG GGCATTTGCT GGGATCCACA GACAGCCAAG TGAAGGAAAA GTCAACCATG 
AAAGCCATCT TTGCAAATTT GCTTCCAGGA AATAGCTATA ATCCTATTCC ATTTCCTTTT 
GATCCAGATA AACACTACTT AATGTATGAA CATGAACGAG TGCCCATTGC AGTCTGCGAG 
AAGGAACCCA GCTCCATCAT TGCTTTTGCT CTCAGTTGTA AAGAATACCG AAATGCCTTA 
GAGGAATTGT CTAAAGCGAC TCAGTGGAAC AGTGCCGAAG AAGGGCTTCC AACAAATAGT 
ACTTCAGATA GCAGACCAAA GAGTAGCAGC CCTATCAGAT TACCTGAAAT GAGTGGAGGA 
CAGACAAATC GTACAACAGA AACAGAACCA CAACCAACCA AAAAGGCTTC TGGAATGTTG 
TCCTTCTTCA GAGGGACAGC AGGGAAAAGC CCCGATCTCT CTTCCCAGAA GAGAGAGACC 
TTACGTGGAG CAGATAGTGC TTACTACCAG GTTGGGCAGA CGGGCAAGGA GGGGACCGAG 
AATCAAGGCG TTGAGCCTCA AGATGAAGTA GATGGAGGAG ATACACAAAA GAAGCAACTC 
ATAAATCCTC ATGTGGAACT TCAATTTTCA GATGCTAATG CCAAGTTTTA CTGTCGGCTC 
TACTATGCGG GAGAGTTTCA TAAGATGCGT GAAGTGATTC TGGACAGCAG TGAAGAAGAT 
TTCATTCGTT CCCTCTCCCA CTCATCACCC TGGCAGGCCC GGGGAGGCAA ATCAGGAGCT 
GCCTTCTATG CAACTGAGGA TGATAGATTT ATTTTGAAGC AAATGCCTCG TCTGGAAGTC 
CAGTCCTTCC TCGACTTTGC ACCACATTAC TTCAATTATA TTACAAATGC TGTTCAACAA 
AAGAGGCCCA CGGCGTTGGC CAAAATTCTT GGAGTTTACA GAATTGGTTA TAAGAACTCT 
CAGAACAACA CTGAGAAGAA GTTAGATCTC CTTGTCATGG AAAATCTTTT CTACGGGAGA 
AAGATGGCAC AGGTTTTTGA TTTGAAGGGC TCTCTTAGGA ATCGGAATGT AAAAACTGAC 
ACTGGAAAAG AGAGTTGTGA TGTGGTCCTG CTAGATGAAA ATCTCCTAAA GATGGTTCGA 
GACAACCCTC TATATATTCG TTCTCATTCC AAAGCTGTGC TGAGAACCTC GATCCATAGT 
GACTCCCATT TCCTTTCTAG CCACCTCATT ATAGATTATT CTTTGCTGGT TGGGCGAGAT 
GATACTAGCA ATGAGCTAGT AGTTGGAATT ATAGATTATA TTCGAACATT TACATGGGAC 
AAAAAGCTTG AGATGGTTGT GAAATCAACA GGAATTTTAG GTGGACAAGG TAAAATGCCA 
ACAGTGGTGT CTCCGGAGTT GTACAGGACT AGGTTTTGTG AGGCAATGGA CAAGTATTTC 
CTAATGGTAC CAGACCACTG GACAGGCTTG GGTCTGAATT GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011509085.1
CDS	173..6337
Translation	MATDDKTSPTLDSANDLPRSPTSPSHLTHFKPLTPDQDEPPFKSAYSSFVNLFRFNKERAEGGQGEQQPLSGSWTSPQLPSRTQSVRSPTPYKKQLNEELQRRSSALADNSLQHPQENTDTRRKAEPTFGGHDPRTAVQLRSLSTVLKRLKEIMEGKSQDSDLKQYWMPDSQCKECYDCSEKFTTFRRRHHCRLCGQIFCSRCCNQEIPGKFMGYTGDLRACTYCRKIALSYAHSTDSNSIGEDLNALSDSACSVSVLDPSEPRTPVGSRKASRNIFLEDDLAWQSLIHPDSSNTPLSTRLVSVQEDAGKSPARNRSASITNLSLDRSGSPMVPSYETSVSPQANRTYVRTETTEDERKILLDSVQLKDLWKKICHHSSGMEFQDHRYWLRTHPNCIVGKELVNWLIRNGHIATRAQAIAIGQAMVDGRWLDCVSHHDQLFRDEYALYRPLQSTEFSETPSPDSDSVNSVEGHSEPSWFKDIKFDDSDTEQIAEEGDDNLAKYLISDTGGQQLSISDAFIKESLFNRRVEEKSKELPFTPLGWHHNNLELLREENGEKQAMERLLSANHNHMMALLQQLLHSDSLSSSWRDIIVSLVCQVVQTVRPDVKNQDDDMDIRQFVHIKKIPGGKKFDSVVVNGFVCTKNIAHKKMSSCIKNPKILLLKCSIEYLYREETKFTCIDPIVLQEREFLKNYVQRIVDVRPTLVLVEKTVSRIAQDMLLEHGITLVINVKSQVLERISRMTQGDLVMSMDQLLTKPHLGTCHKFYMQIFQLPNEQTKTLMFFEGCPQHLGCTIKLRGGSDYELARVKEILIFMICVAYHSQLEISFLMDEFAMPPTLMQNPSFHSLIEGRGHEGAVQEQYGGGSIPWDPDIPPESLPCDDSSLLELRIVFEKGEQENKNLPQAVASVKHQEHSTTACPAGLPCAFFAPVPESLLPLPVDDQQDALGSEQPETLQQTVVLQDPKSQIRAFRDPLQDDTGLYVTEEVTSSEDKRKTYSLAFKQELKDVILCISPVITFREPFLLTEKGMRCSTRDYFAEQVYWSPLLNKEFKEMENRRKKQLLRDLSGLQGMNGSIQAKSIQVLPSHELVSTRIAEHLGDSQSLGRMLADYRARGGRIQPKNSDPFAHSKDASSTSSGQSGSKNEGDEERGLILSDAVWSTKVDCLNPINHQRLCVLFSSSSAQSSNAPSACVSPWIVTMEFYGKNDLTLGIFLERYCFRPSYQCPSMFCDTPMVHHIRRFVHGQGCVQIILKELDSPVPGYQHTILTYSWCRICKQVTPVVALSNESWSMSFAKYLELRFYGHQYTRRANAEPCGHSIHHDYHQYFSYNQMVASFSYSPIRLLEVCVPLPKIFIKRQAPLKVSLLQDLKDFFQKVSQVYVAIDERLASLKTDTFSKTREEKMEDIFAQKEMEEGEFKNWIEKMQARLMSSSVDTPQQLQSVFESLIAKKQSLCEVLQAWNNRLQDLFQQEKGRKRPSVPPSPGRLRQGEESKISAMDASPRNISPGLQNGEKEDRFLTTLSSQSSTSSTHLQLPTPPEVMSEQSVGGPPELDTASSSEDVFDGHLLGSTDSQVKEKSTMKAIFANLLPGNSYNPIPFPFDPDKHYLMYEHERVPIAVCEKEPSSIIAFALSCKEYRNALEELSKATQWNSAEEGLPTNSTSDSRPKSSSPIRLPEMSGGQTNRTTETEPQPTKKASGMLSFFRGTAGKSPDLSSQKRETLRGADSAYYQVGQTGKEGTENQGVEPQDEVDGGDTQKKQLINPHVELQFSDANAKFYCRLYYAGEFHKMREVILDSSEEDFIRSLSHSSPWQARGGKSGAAFYATEDDRFILKQMPRLEVQSFLDFAPHYFNYITNAVQQKRPTALAKILGVYRIGYKNSQNNTEKKLDLLVMENLFYGRKMAQVFDLKGSLRNRNVKTDTGKESCDVVLLDENLLKMVRDNPLYIRSHSKAVLRTSIHSDSHFLSSHLIIDYSLLVGRDDTSNELVVGIIDYIRTFTWDKKLEMVVKSTGILGGQGKMPTVVSPELYRTRFCEAMDKYFLMVPDHWTGLGLNC

Target ORF information:

RefSeq Version	XM_011510783.2
Organism	Homo sapiens(human)
Definition	Homo sapiens phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011510783.2

ORF Insert Sequence:

ATGGCCACAG ATGATAAGAC GTCCCCAACA CTGGACTCTG CTAATGATTT GCCTCGATCT 
CCTACTAGTC CTTCTCATCT CACACACTTT AAACCTTTGA CTCCTGATCA AGATGAGCCC 
CCTTTTAAAT CAGCTTATAG TTCTTTTGTA AATCTCTTTC GTTTTAACAA AGAGAGAGCA 
GAAGGAGGCC AGGGAGAACA GCAGCCTTTG AGTGGAAGTT GGACCAGCCC TCAGCTCCCT 
TCGAGGACAC AGTCTGTTAG GTCACCCACA CCTTATAAAA AGCAGCTTAA TGAGGAACTC 
CAGCGGCGCT CTTCAGCATT AGCAGACAAC AGTTTGCAAC ATCCCCAGGA GAACACAGAC 
ACAAGAAGGA AAGCAGAACC TACCTTTGGA GGTCATGACC CTCGTACAGC TGTTCAGCTT 
CGAAGCCTCA GCACAGTATT AAAACGCCTC AAGGAAATCA TGGAGGGGAA AAGCCAGGAT 
AGTGACCTGA AACAATACTG GATGCCAGAT AGCCAATGTA AAGAGTGCTA TGACTGTAGT 
GAGAAATTTA CAACCTTTAG GCGCAGACAC CATTGCCGAC TATGTGGGCA GATTTTCTGC 
AGTCGTTGCT GTAATCAAGA AATCCCTGGA AAATTTATGG GCTATACAGG AGACCTCCGA 
GCTTGCACAT ATTGTAGAAA AATAGCCTTA AGTTATGCTC ATTCCACAGA CAGTAATTCT 
ATTGGGGAAG ACTTGAATGC TCTTTCAGAT TCTGCTTGCT CTGTGTCTGT GCTTGATCCA 
AGTGAACCCC GAACACCTGT TGGGAGTAGG AAAGCCAGCC GTAACATATT TTTAGAGGAT 
GATTTGGCCT GGCAAAGTTT GATTCATCCA GATTCCTCAA ATACTCCTCT TTCAACAAGA 
CTTGTATCTG TGCAAGAGGA TGCTGGGAAA TCTCCTGCTC GAAATAGATC AGCCAGCATT 
ACTAACCTGT CACTGGATAG ATCTGGTTCT CCTATGGTAC CTTCATATGA GACATCTGTC 
AGTCCCCAGG CTAACCGAAC ATATGTTAGG ACAGAGACCA CTGAGGATGA ACGCAAAATT 
CTTCTGGACA GTGTGCAGTT AAAAGACCTG TGGAAAAAAA TCTGCCATCA CAGCAGTGGA 
ATGGAGTTTC AGGATCACCG CTACTGGTTG AGAACGCATC CCAACTGCAT TGTAGGAAAG 
GAATTAGTCA ACTGGCTAAT CCGAAATGGG CATATTGCCA CAAGGGCACA AGCTATAGCA 
ATTGGACAAG CAATGGTTGA TGGACGTTGG CTGGATTGTG TTAGTCATCA CGACCAGCTT 
TTCAGAGATG AGTATGCGCT GTATAGACCA CTGCAGAGTA CAGAATTTTC TGAGACGCCT 
TCTCCCGACA GTGACTCAGT GAACTCCGTG GAAGGACACT CTGAGCCATC CTGGTTTAAA 
GACATAAAGT TTGATGACAG TGACACAGAA CAGATAGCTG AAGAAGGTGA CGATAATTTG 
GCTAAGTATT TGATTTCTGA CACTGGAGGA CAACAGCTCT CAATAAGTGA CGCTTTCATC 
AAAGAATCCT TATTTAATCG CCGAGTAGAG GAAAAATCCA AAGAGCTGCC TTTCACACCT 
TTGGGCTGGC ATCATAACAA CCTGGAGCTC CTGAGGGAGG AGAATGGGGA GAAACAAGCC 
ATGGAGAGGT TGCTTTCAGC TAATCATAAC CACATGATGG CACTACTCCA GCAGTTGCTC 
CATAGTGACT CACTGTCATC ATCTTGGAGG GACATCATCG TGTCATTGGT CTGCCAGGTT 
GTTCAGACAG TCCGACCTGA TGTCAAGAAC CAGGATGATG ACATGGATAT CCGTCAGTTT 
GTCCACATCA AAAAAATCCC AGGTGGAAAG AAGTTTGATT CTGTGGTTGT CAATGGCTTT 
GTTTGTACCA AGAACATTGC ACATAAAAAG ATGAGTTCTT GTATTAAAAA CCCCAAAATT 
CTTCTGTTGA AGTGTTCCAT TGAGTATCTC TACAGAGAAG AAACTAAGTT TACTTGCATT 
GATCCTATTG TGCTTCAGGA AAGGGAATTC TTGAAGAATT ATGTCCAGCG AATAGTTGAT 
GTTCGACCCA CCTTGGTTCT TGTTGAGAAA ACAGTGTCTC GGATTGCCCA GGACATGTTA 
TTGGAACATG GCATTACTTT GGTCATTAAT GTAAAGTCAC AAGTTTTGGA ACGAATCAGT 
CGAATGACCC AAGGTGATTT AGTGATGTCA ATGGACCAGC TGCTTACGAA ACCACACCTG 
GGCACTTGTC ACAAATTTTA TATGCAGATA TTTCAGTTGC CTAATGAACA AACCAAGACA 
CTGATGTTTT TTGAAGGTTG TCCACAGCAC CTAGGCTGTA CAATCAAGCT AAGAGGAGGC 
TCTGATTATG AGCTGGCTCG AGTTAAGGAG ATCCTAATAT TTATGATCTG TGTTGCTTAT 
CATTCTCAAC TAGAAATATC CTTTCTCATG GATGAATTTG CTATGCCTCC CACATTAATG 
CAAAACCCTT CATTCCATTC CCTGATTGAG GGACGAGGGC ATGAGGGGGC TGTCCAAGAG 
CAGTACGGTG GAGGTTCCAT CCCCTGGGAT CCTGACATCC CTCCTGAGTC TCTGCCCTGT 
GATGATAGCA GTTTGCTGGA ATTGAGGATT GTGTTTGAGA AGGGTGAGCA GGAAAATAAA 
AATCTTCCGC AGGCTGTTGC CTCTGTGAAG CATCAAGAAC ATAGCACAAC AGCTTGCCCG 
GCGGGTCTCC CTTGTGCTTT CTTTGCACCT GTACCGGAAT CATTGTTGCC ACTCCCTGTG 
GATGACCAAC AAGATGCTTT AGGCAGCGAG CAGCCAGAGA CTTTGCAGCA AACAGTTGTG 
CTGCAGGATC CCAAAAGCCA GATAAGAGCC TTTAGAGACC CTCTACAGGA TGACACTGGA 
TTATATGTTA CTGAGGAAGT CACCTCCTCT GAAGATAAAC GAAAGACTTA TTCTTTGGCC 
TTTAAGCAGG AATTAAAAGA TGTGATCCTC TGTATCTCCC CAGTAATCAC ATTCCGAGAA 
CCCTTTCTTT TAACTGAAAA GGGGATGAGA TGCTCTACCC GAGATTATTT TGCAGAGCAG 
GTTTACTGGT CTCCTCTCCT CAATAAAGAA TTCAAAGAAA TGGAGAACAG GAGGAAGAAA 
CAGCTGCTCA GGGATCTCTC TGGACTTCAG GGCATGAATG GAAGTATTCA GGCCAAGTCT 
ATTCAAGTCT TACCCTCACA TGAGCTAGTG AGCACTAGAA TTGCTGAGCA TCTGGGCGAT 
AGCCAGAGCT TGGGTAGAAT GCTGGCCGAT TATCGAGCCA GAGGAGGAAG AATTCAGCCC 
AAAAATTCAG ACCCTTTTGC TCATTCAAAG GATGCATCAA GTACTTCAAG TGGCCAATCA 
GGAAGCAAAA ATGAGGGTGA TGAAGAGAGA GGGCTTATTC TGAGTGATGC TGTGTGGTCA 
ACAAAGGTGG ACTGTCTGAA TCCCATTAAT CACCAGAGAC TTTGTGTGCT CTTCAGCAGC 
TCTTCTGCCC AGTCCAGCAA TGCTCCTAGT GCCTGTGTCA GTCCTTGGAT TGTAACAATG 
GAATTTTATG GAAAGAATGA TCTTACATTA GGAATATTTT TAGAGAGATA CTGTTTCAGG 
CCTTCTTATC AGTGTCCAAG CATGTTCTGT GATACCCCCA TGGTACATCA TATTCGGCGC 
TTTGTTCATG GCCAAGGCTG TGTGCAGATA ATCCTGAAGG AGTTGGATTC TCCAGTACCT 
GGATATCAGC ATACAATTCT TACATATTCC TGGTGTAGAA TCTGCAAACA GGTAACACCA 
GTTGTTGCTC TTTCCAATGA GTCCTGGTCT ATGTCATTTG CAAAATACCT TGAACTTAGG 
TTTTATGGGC ACCAGTATAC TCGCAGAGCC AACGCTGAGC CCTGTGGTCA CTCCATCCAT 
CATGATTATC ACCAGTATTT CTCCTATAAC CAGATGGTGG CGTCTTTCAG TTATTCTCCC 
ATTCGGCTTC TTGAAGTATG TGTTCCACTC CCCAAAATAT TCATTAAGCG TCAGGCCCCA 
TTAAAAGTGT CCCTTCTTCA GGATCTGAAG GACTTCTTTC AAAAAGTTTC ACAGGTATAT 
GTTGCCATTG ATGAAAGACT TGCATCTTTG AAAACTGATA CATTTAGTAA AACAAGAGAG 
GAAAAAATGG AAGATATTTT TGCACAGAAA GAGATGGAAG AAGGTGAGTT CAAGAACTGG 
ATTGAGAAGA TGCAAGCAAG GCTCATGTCT TCCTCTGTAG ATACCCCTCA GCAACTGCAG 
TCGGTCTTTG AGTCACTCAT TGCCAAGAAA CAAAGTCTCT GTGAAGTGCT GCAAGCTTGG 
AATAACAGGT TGCAGGACCT TTTCCAACAG GAAAAGGGTA GAAAGAGACC TTCAGTTCCT 
CCAAGTCCTG GAAGACTGAG ACAAGGGGAA GAAAGCAAGA TAAGTGCGAT GGATGCATCT 
CCACGGAATA TTTCTCCAGG ACTTCAGAAT GGAGAAAAAG AGGATCGCTT CTTAACAACT 
TTGTCCAGCC AGAGCTCCAC CAGTTCTACT CATCTCCAAT TGCCTACGCC ACCTGAAGTC 
ATGTCTGAAC AGTCAGTGGG AGGGCCCCCT GAGCTAGATA CAGCCAGCAG TTCCGAAGAT 
GTGTTTGATG GGCATTTGCT GGGATCCACA GACAGCCAAG TGAAGGAAAA GTCAACCATG 
AAAGCCATCT TTGCAAATTT GCTTCCAGGA AATAGCTATA ATCCTATTCC ATTTCCTTTT 
GATCCAGATA AACACTACTT AATGTATGAA CATGAACGAG TGCCCATTGC AGTCTGCGAG 
AAGGAACCCA GCTCCATCAT TGCTTTTGCT CTCAGTTGTA AAGAATACCG AAATGCCTTA 
GAGGAATTGT CTAAAGCGAC TCAGTGGAAC AGTGCCGAAG AAGGGCTTCC AACAAATAGT 
ACTTCAGATA GCAGACCAAA GAGTAGCAGC CCTATCAGAT TACCTGAAAT GAGTGGAGGA 
CAGACAAATC GTACAACAGA AACAGAACCA CAACCAACCA AAAAGGCTTC TGGAATGTTG 
TCCTTCTTCA GAGGGACAGC AGGGAAAAGC CCCGATCTCT CTTCCCAGAA GAGAGAGACC 
TTACGTGGAG CAGATAGTGC TTACTACCAG GTTGGGCAGA CGGGCAAGGA GGGGACCGAG 
AATCAAGGCG TTGAGCCTCA AGATGAAGTA GATGGAGGAG ATACACAAAA GAAGCAACTC 
ATAAATCCTC ATGTGGAACT TCAATTTTCA GATGCTAATG CCAAGTTTTA CTGTCGGCTC 
TACTATGCGG GAGAGTTTCA TAAGATGCGT GAAGTGATTC TGGACAGCAG TGAAGAAGAT 
TTCATTCGTT CCCTCTCCCA CTCATCACCC TGGCAGGCCC GGGGAGGCAA ATCAGGAGCT 
GCCTTCTATG CAACTGAGGA TGATAGATTT ATTTTGAAGC AAATGCCTCG TCTGGAAGTC 
CAGTCCTTCC TCGACTTTGC ACCACATTAC TTCAATTATA TTACAAATGC TGTTCAACAA 
AAGAGGCCCA CGGCGTTGGC CAAAATTCTT GGAGTTTACA GAATTGGTTA TAAGAACTCT 
CAGAACAACA CTGAGAAGAA GTTAGATCTC CTTGTCATGG AAAATCTTTT CTACGGGAGA 
AAGATGGCAC AGGTTTTTGA TTTGAAGGGC TCTCTTAGGA ATCGGAATGT AAAAACTGAC 
ACTGGAAAAG AGAGTTGTGA TGTGGTCCTG CTAGATGAAA ATCTCCTAAA GATGGTTCGA 
GACAACCCTC TATATATTCG TTCTCATTCC AAAGCTGTGC TGAGAACCTC GATCCATAGT 
GACTCCCATT TCCTTTCTAG CCACCTCATT ATAGATTATT CTTTGCTGGT TGGGCGAGAT 
GATACTAGCA ATGAGCTAGT AGTTGGAATT ATAGATTATA TTCGAACATT TACATGGGAC 
AAAAAGCTTG AGATGGTTGT GAAATCAACA GGAATTTTAG GTGGACAAGG TAAAATGCCA 
ACAGTGGTGT CTCCGGAGTT GTACAGGACT AGGTTTTGTG AGGCAATGGA CAAGTATTTC 
CTAATGGTAC CAGACCACTG GACAGGCTTG GGTCTGAATT GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PIKFYVE ( XM_011510783.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011509085.1 Homo sapiens phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X7, mRNA.

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CloneID	OHu77665
Clone ID Related Accession (Same CDS sequence)	XM_011510783.2 , XM_011510783.3
Accession Version	XM_011510783.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6165bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	1-phosphatidylinositol 3-phosphate 5-kinase isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011510783.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG ATGATAAGAC GTCCCCAACA CTGGACTCTG CTAATGATTT GCCTCGATCT 
CCTACTAGTC CTTCTCATCT CACACACTTT AAACCTTTGA CTCCTGATCA AGATGAGCCC 
CCTTTTAAAT CAGCTTATAG TTCTTTTGTA AATCTCTTTC GTTTTAACAA AGAGAGAGCA 
GAAGGAGGCC AGGGAGAACA GCAGCCTTTG AGTGGAAGTT GGACCAGCCC TCAGCTCCCT 
TCGAGGACAC AGTCTGTTAG GTCACCCACA CCTTATAAAA AGCAGCTTAA TGAGGAACTC 
CAGCGGCGCT CTTCAGCATT AGCAGACAAC AGTTTGCAAC ATCCCCAGGA GAACACAGAC 
ACAAGAAGGA AAGCAGAACC TACCTTTGGA GGTCATGACC CTCGTACAGC TGTTCAGCTT 
CGAAGCCTCA GCACAGTATT AAAACGCCTC AAGGAAATCA TGGAGGGGAA AAGCCAGGAT 
AGTGACCTGA AACAATACTG GATGCCAGAT AGCCAATGTA AAGAGTGCTA TGACTGTAGT 
GAGAAATTTA CAACCTTTAG GCGCAGACAC CATTGCCGAC TATGTGGGCA GATTTTCTGC 
AGTCGTTGCT GTAATCAAGA AATCCCTGGA AAATTTATGG GCTATACAGG AGACCTCCGA 
GCTTGCACAT ATTGTAGAAA AATAGCCTTA AGTTATGCTC ATTCCACAGA CAGTAATTCT 
ATTGGGGAAG ACTTGAATGC TCTTTCAGAT TCTGCTTGCT CTGTGTCTGT GCTTGATCCA 
AGTGAACCCC GAACACCTGT TGGGAGTAGG AAAGCCAGCC GTAACATATT TTTAGAGGAT 
GATTTGGCCT GGCAAAGTTT GATTCATCCA GATTCCTCAA ATACTCCTCT TTCAACAAGA 
CTTGTATCTG TGCAAGAGGA TGCTGGGAAA TCTCCTGCTC GAAATAGATC AGCCAGCATT 
ACTAACCTGT CACTGGATAG ATCTGGTTCT CCTATGGTAC CTTCATATGA GACATCTGTC 
AGTCCCCAGG CTAACCGAAC ATATGTTAGG ACAGAGACCA CTGAGGATGA ACGCAAAATT 
CTTCTGGACA GTGTGCAGTT AAAAGACCTG TGGAAAAAAA TCTGCCATCA CAGCAGTGGA 
ATGGAGTTTC AGGATCACCG CTACTGGTTG AGAACGCATC CCAACTGCAT TGTAGGAAAG 
GAATTAGTCA ACTGGCTAAT CCGAAATGGG CATATTGCCA CAAGGGCACA AGCTATAGCA 
ATTGGACAAG CAATGGTTGA TGGACGTTGG CTGGATTGTG TTAGTCATCA CGACCAGCTT 
TTCAGAGATG AGTATGCGCT GTATAGACCA CTGCAGAGTA CAGAATTTTC TGAGACGCCT 
TCTCCCGACA GTGACTCAGT GAACTCCGTG GAAGGACACT CTGAGCCATC CTGGTTTAAA 
GACATAAAGT TTGATGACAG TGACACAGAA CAGATAGCTG AAGAAGGTGA CGATAATTTG 
GCTAAGTATT TGATTTCTGA CACTGGAGGA CAACAGCTCT CAATAAGTGA CGCTTTCATC 
AAAGAATCCT TATTTAATCG CCGAGTAGAG GAAAAATCCA AAGAGCTGCC TTTCACACCT 
TTGGGCTGGC ATCATAACAA CCTGGAGCTC CTGAGGGAGG AGAATGGGGA GAAACAAGCC 
ATGGAGAGGT TGCTTTCAGC TAATCATAAC CACATGATGG CACTACTCCA GCAGTTGCTC 
CATAGTGACT CACTGTCATC ATCTTGGAGG GACATCATCG TGTCATTGGT CTGCCAGGTT 
GTTCAGACAG TCCGACCTGA TGTCAAGAAC CAGGATGATG ACATGGATAT CCGTCAGTTT 
GTCCACATCA AAAAAATCCC AGGTGGAAAG AAGTTTGATT CTGTGGTTGT CAATGGCTTT 
GTTTGTACCA AGAACATTGC ACATAAAAAG ATGAGTTCTT GTATTAAAAA CCCCAAAATT 
CTTCTGTTGA AGTGTTCCAT TGAGTATCTC TACAGAGAAG AAACTAAGTT TACTTGCATT 
GATCCTATTG TGCTTCAGGA AAGGGAATTC TTGAAGAATT ATGTCCAGCG AATAGTTGAT 
GTTCGACCCA CCTTGGTTCT TGTTGAGAAA ACAGTGTCTC GGATTGCCCA GGACATGTTA 
TTGGAACATG GCATTACTTT GGTCATTAAT GTAAAGTCAC AAGTTTTGGA ACGAATCAGT 
CGAATGACCC AAGGTGATTT AGTGATGTCA ATGGACCAGC TGCTTACGAA ACCACACCTG 
GGCACTTGTC ACAAATTTTA TATGCAGATA TTTCAGTTGC CTAATGAACA AACCAAGACA 
CTGATGTTTT TTGAAGGTTG TCCACAGCAC CTAGGCTGTA CAATCAAGCT AAGAGGAGGC 
TCTGATTATG AGCTGGCTCG AGTTAAGGAG ATCCTAATAT TTATGATCTG TGTTGCTTAT 
CATTCTCAAC TAGAAATATC CTTTCTCATG GATGAATTTG CTATGCCTCC CACATTAATG 
CAAAACCCTT CATTCCATTC CCTGATTGAG GGACGAGGGC ATGAGGGGGC TGTCCAAGAG 
CAGTACGGTG GAGGTTCCAT CCCCTGGGAT CCTGACATCC CTCCTGAGTC TCTGCCCTGT 
GATGATAGCA GTTTGCTGGA ATTGAGGATT GTGTTTGAGA AGGGTGAGCA GGAAAATAAA 
AATCTTCCGC AGGCTGTTGC CTCTGTGAAG CATCAAGAAC ATAGCACAAC AGCTTGCCCG 
GCGGGTCTCC CTTGTGCTTT CTTTGCACCT GTACCGGAAT CATTGTTGCC ACTCCCTGTG 
GATGACCAAC AAGATGCTTT AGGCAGCGAG CAGCCAGAGA CTTTGCAGCA AACAGTTGTG 
CTGCAGGATC CCAAAAGCCA GATAAGAGCC TTTAGAGACC CTCTACAGGA TGACACTGGA 
TTATATGTTA CTGAGGAAGT CACCTCCTCT GAAGATAAAC GAAAGACTTA TTCTTTGGCC 
TTTAAGCAGG AATTAAAAGA TGTGATCCTC TGTATCTCCC CAGTAATCAC ATTCCGAGAA 
CCCTTTCTTT TAACTGAAAA GGGGATGAGA TGCTCTACCC GAGATTATTT TGCAGAGCAG 
GTTTACTGGT CTCCTCTCCT CAATAAAGAA TTCAAAGAAA TGGAGAACAG GAGGAAGAAA 
CAGCTGCTCA GGGATCTCTC TGGACTTCAG GGCATGAATG GAAGTATTCA GGCCAAGTCT 
ATTCAAGTCT TACCCTCACA TGAGCTAGTG AGCACTAGAA TTGCTGAGCA TCTGGGCGAT 
AGCCAGAGCT TGGGTAGAAT GCTGGCCGAT TATCGAGCCA GAGGAGGAAG AATTCAGCCC 
AAAAATTCAG ACCCTTTTGC TCATTCAAAG GATGCATCAA GTACTTCAAG TGGCCAATCA 
GGAAGCAAAA ATGAGGGTGA TGAAGAGAGA GGGCTTATTC TGAGTGATGC TGTGTGGTCA 
ACAAAGGTGG ACTGTCTGAA TCCCATTAAT CACCAGAGAC TTTGTGTGCT CTTCAGCAGC 
TCTTCTGCCC AGTCCAGCAA TGCTCCTAGT GCCTGTGTCA GTCCTTGGAT TGTAACAATG 
GAATTTTATG GAAAGAATGA TCTTACATTA GGAATATTTT TAGAGAGATA CTGTTTCAGG 
CCTTCTTATC AGTGTCCAAG CATGTTCTGT GATACCCCCA TGGTACATCA TATTCGGCGC 
TTTGTTCATG GCCAAGGCTG TGTGCAGATA ATCCTGAAGG AGTTGGATTC TCCAGTACCT 
GGATATCAGC ATACAATTCT TACATATTCC TGGTGTAGAA TCTGCAAACA GGTAACACCA 
GTTGTTGCTC TTTCCAATGA GTCCTGGTCT ATGTCATTTG CAAAATACCT TGAACTTAGG 
TTTTATGGGC ACCAGTATAC TCGCAGAGCC AACGCTGAGC CCTGTGGTCA CTCCATCCAT 
CATGATTATC ACCAGTATTT CTCCTATAAC CAGATGGTGG CGTCTTTCAG TTATTCTCCC 
ATTCGGCTTC TTGAAGTATG TGTTCCACTC CCCAAAATAT TCATTAAGCG TCAGGCCCCA 
TTAAAAGTGT CCCTTCTTCA GGATCTGAAG GACTTCTTTC AAAAAGTTTC ACAGGTATAT 
GTTGCCATTG ATGAAAGACT TGCATCTTTG AAAACTGATA CATTTAGTAA AACAAGAGAG 
GAAAAAATGG AAGATATTTT TGCACAGAAA GAGATGGAAG AAGGTGAGTT CAAGAACTGG 
ATTGAGAAGA TGCAAGCAAG GCTCATGTCT TCCTCTGTAG ATACCCCTCA GCAACTGCAG 
TCGGTCTTTG AGTCACTCAT TGCCAAGAAA CAAAGTCTCT GTGAAGTGCT GCAAGCTTGG 
AATAACAGGT TGCAGGACCT TTTCCAACAG GAAAAGGGTA GAAAGAGACC TTCAGTTCCT 
CCAAGTCCTG GAAGACTGAG ACAAGGGGAA GAAAGCAAGA TAAGTGCGAT GGATGCATCT 
CCACGGAATA TTTCTCCAGG ACTTCAGAAT GGAGAAAAAG AGGATCGCTT CTTAACAACT 
TTGTCCAGCC AGAGCTCCAC CAGTTCTACT CATCTCCAAT TGCCTACGCC ACCTGAAGTC 
ATGTCTGAAC AGTCAGTGGG AGGGCCCCCT GAGCTAGATA CAGCCAGCAG TTCCGAAGAT 
GTGTTTGATG GGCATTTGCT GGGATCCACA GACAGCCAAG TGAAGGAAAA GTCAACCATG 
AAAGCCATCT TTGCAAATTT GCTTCCAGGA AATAGCTATA ATCCTATTCC ATTTCCTTTT 
GATCCAGATA AACACTACTT AATGTATGAA CATGAACGAG TGCCCATTGC AGTCTGCGAG 
AAGGAACCCA GCTCCATCAT TGCTTTTGCT CTCAGTTGTA AAGAATACCG AAATGCCTTA 
GAGGAATTGT CTAAAGCGAC TCAGTGGAAC AGTGCCGAAG AAGGGCTTCC AACAAATAGT 
ACTTCAGATA GCAGACCAAA GAGTAGCAGC CCTATCAGAT TACCTGAAAT GAGTGGAGGA 
CAGACAAATC GTACAACAGA AACAGAACCA CAACCAACCA AAAAGGCTTC TGGAATGTTG 
TCCTTCTTCA GAGGGACAGC AGGGAAAAGC CCCGATCTCT CTTCCCAGAA GAGAGAGACC 
TTACGTGGAG CAGATAGTGC TTACTACCAG GTTGGGCAGA CGGGCAAGGA GGGGACCGAG 
AATCAAGGCG TTGAGCCTCA AGATGAAGTA GATGGAGGAG ATACACAAAA GAAGCAACTC 
ATAAATCCTC ATGTGGAACT TCAATTTTCA GATGCTAATG CCAAGTTTTA CTGTCGGCTC 
TACTATGCGG GAGAGTTTCA TAAGATGCGT GAAGTGATTC TGGACAGCAG TGAAGAAGAT 
TTCATTCGTT CCCTCTCCCA CTCATCACCC TGGCAGGCCC GGGGAGGCAA ATCAGGAGCT 
GCCTTCTATG CAACTGAGGA TGATAGATTT ATTTTGAAGC AAATGCCTCG TCTGGAAGTC 
CAGTCCTTCC TCGACTTTGC ACCACATTAC TTCAATTATA TTACAAATGC TGTTCAACAA 
AAGAGGCCCA CGGCGTTGGC CAAAATTCTT GGAGTTTACA GAATTGGTTA TAAGAACTCT 
CAGAACAACA CTGAGAAGAA GTTAGATCTC CTTGTCATGG AAAATCTTTT CTACGGGAGA 
AAGATGGCAC AGGTTTTTGA TTTGAAGGGC TCTCTTAGGA ATCGGAATGT AAAAACTGAC 
ACTGGAAAAG AGAGTTGTGA TGTGGTCCTG CTAGATGAAA ATCTCCTAAA GATGGTTCGA 
GACAACCCTC TATATATTCG TTCTCATTCC AAAGCTGTGC TGAGAACCTC GATCCATAGT 
GACTCCCATT TCCTTTCTAG CCACCTCATT ATAGATTATT CTTTGCTGGT TGGGCGAGAT 
GATACTAGCA ATGAGCTAGT AGTTGGAATT ATAGATTATA TTCGAACATT TACATGGGAC 
AAAAAGCTTG AGATGGTTGT GAAATCAACA GGAATTTTAG GTGGACAAGG TAAAATGCCA 
ACAGTGGTGT CTCCGGAGTT GTACAGGACT AGGTTTTGTG AGGCAATGGA CAAGTATTTC 
CTAATGGTAC CAGACCACTG GACAGGCTTG GGTCTGAATT GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011509085.1
CDS	182..6346
Translation	MATDDKTSPTLDSANDLPRSPTSPSHLTHFKPLTPDQDEPPFKSAYSSFVNLFRFNKERAEGGQGEQQPLSGSWTSPQLPSRTQSVRSPTPYKKQLNEELQRRSSALADNSLQHPQENTDTRRKAEPTFGGHDPRTAVQLRSLSTVLKRLKEIMEGKSQDSDLKQYWMPDSQCKECYDCSEKFTTFRRRHHCRLCGQIFCSRCCNQEIPGKFMGYTGDLRACTYCRKIALSYAHSTDSNSIGEDLNALSDSACSVSVLDPSEPRTPVGSRKASRNIFLEDDLAWQSLIHPDSSNTPLSTRLVSVQEDAGKSPARNRSASITNLSLDRSGSPMVPSYETSVSPQANRTYVRTETTEDERKILLDSVQLKDLWKKICHHSSGMEFQDHRYWLRTHPNCIVGKELVNWLIRNGHIATRAQAIAIGQAMVDGRWLDCVSHHDQLFRDEYALYRPLQSTEFSETPSPDSDSVNSVEGHSEPSWFKDIKFDDSDTEQIAEEGDDNLAKYLISDTGGQQLSISDAFIKESLFNRRVEEKSKELPFTPLGWHHNNLELLREENGEKQAMERLLSANHNHMMALLQQLLHSDSLSSSWRDIIVSLVCQVVQTVRPDVKNQDDDMDIRQFVHIKKIPGGKKFDSVVVNGFVCTKNIAHKKMSSCIKNPKILLLKCSIEYLYREETKFTCIDPIVLQEREFLKNYVQRIVDVRPTLVLVEKTVSRIAQDMLLEHGITLVINVKSQVLERISRMTQGDLVMSMDQLLTKPHLGTCHKFYMQIFQLPNEQTKTLMFFEGCPQHLGCTIKLRGGSDYELARVKEILIFMICVAYHSQLEISFLMDEFAMPPTLMQNPSFHSLIEGRGHEGAVQEQYGGGSIPWDPDIPPESLPCDDSSLLELRIVFEKGEQENKNLPQAVASVKHQEHSTTACPAGLPCAFFAPVPESLLPLPVDDQQDALGSEQPETLQQTVVLQDPKSQIRAFRDPLQDDTGLYVTEEVTSSEDKRKTYSLAFKQELKDVILCISPVITFREPFLLTEKGMRCSTRDYFAEQVYWSPLLNKEFKEMENRRKKQLLRDLSGLQGMNGSIQAKSIQVLPSHELVSTRIAEHLGDSQSLGRMLADYRARGGRIQPKNSDPFAHSKDASSTSSGQSGSKNEGDEERGLILSDAVWSTKVDCLNPINHQRLCVLFSSSSAQSSNAPSACVSPWIVTMEFYGKNDLTLGIFLERYCFRPSYQCPSMFCDTPMVHHIRRFVHGQGCVQIILKELDSPVPGYQHTILTYSWCRICKQVTPVVALSNESWSMSFAKYLELRFYGHQYTRRANAEPCGHSIHHDYHQYFSYNQMVASFSYSPIRLLEVCVPLPKIFIKRQAPLKVSLLQDLKDFFQKVSQVYVAIDERLASLKTDTFSKTREEKMEDIFAQKEMEEGEFKNWIEKMQARLMSSSVDTPQQLQSVFESLIAKKQSLCEVLQAWNNRLQDLFQQEKGRKRPSVPPSPGRLRQGEESKISAMDASPRNISPGLQNGEKEDRFLTTLSSQSSTSSTHLQLPTPPEVMSEQSVGGPPELDTASSSEDVFDGHLLGSTDSQVKEKSTMKAIFANLLPGNSYNPIPFPFDPDKHYLMYEHERVPIAVCEKEPSSIIAFALSCKEYRNALEELSKATQWNSAEEGLPTNSTSDSRPKSSSPIRLPEMSGGQTNRTTETEPQPTKKASGMLSFFRGTAGKSPDLSSQKRETLRGADSAYYQVGQTGKEGTENQGVEPQDEVDGGDTQKKQLINPHVELQFSDANAKFYCRLYYAGEFHKMREVILDSSEEDFIRSLSHSSPWQARGGKSGAAFYATEDDRFILKQMPRLEVQSFLDFAPHYFNYITNAVQQKRPTALAKILGVYRIGYKNSQNNTEKKLDLLVMENLFYGRKMAQVFDLKGSLRNRNVKTDTGKESCDVVLLDENLLKMVRDNPLYIRSHSKAVLRTSIHSDSHFLSSHLIIDYSLLVGRDDTSNELVVGIIDYIRTFTWDKKLEMVVKSTGILGGQGKMPTVVSPELYRTRFCEAMDKYFLMVPDHWTGLGLNC

Target ORF information:

RefSeq Version	XM_011510783.3
Organism	Homo sapiens(human)
Definition	Homo sapiens phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011510783.3

ORF Insert Sequence:

ATGGCCACAG ATGATAAGAC GTCCCCAACA CTGGACTCTG CTAATGATTT GCCTCGATCT 
CCTACTAGTC CTTCTCATCT CACACACTTT AAACCTTTGA CTCCTGATCA AGATGAGCCC 
CCTTTTAAAT CAGCTTATAG TTCTTTTGTA AATCTCTTTC GTTTTAACAA AGAGAGAGCA 
GAAGGAGGCC AGGGAGAACA GCAGCCTTTG AGTGGAAGTT GGACCAGCCC TCAGCTCCCT 
TCGAGGACAC AGTCTGTTAG GTCACCCACA CCTTATAAAA AGCAGCTTAA TGAGGAACTC 
CAGCGGCGCT CTTCAGCATT AGCAGACAAC AGTTTGCAAC ATCCCCAGGA GAACACAGAC 
ACAAGAAGGA AAGCAGAACC TACCTTTGGA GGTCATGACC CTCGTACAGC TGTTCAGCTT 
CGAAGCCTCA GCACAGTATT AAAACGCCTC AAGGAAATCA TGGAGGGGAA AAGCCAGGAT 
AGTGACCTGA AACAATACTG GATGCCAGAT AGCCAATGTA AAGAGTGCTA TGACTGTAGT 
GAGAAATTTA CAACCTTTAG GCGCAGACAC CATTGCCGAC TATGTGGGCA GATTTTCTGC 
AGTCGTTGCT GTAATCAAGA AATCCCTGGA AAATTTATGG GCTATACAGG AGACCTCCGA 
GCTTGCACAT ATTGTAGAAA AATAGCCTTA AGTTATGCTC ATTCCACAGA CAGTAATTCT 
ATTGGGGAAG ACTTGAATGC TCTTTCAGAT TCTGCTTGCT CTGTGTCTGT GCTTGATCCA 
AGTGAACCCC GAACACCTGT TGGGAGTAGG AAAGCCAGCC GTAACATATT TTTAGAGGAT 
GATTTGGCCT GGCAAAGTTT GATTCATCCA GATTCCTCAA ATACTCCTCT TTCAACAAGA 
CTTGTATCTG TGCAAGAGGA TGCTGGGAAA TCTCCTGCTC GAAATAGATC AGCCAGCATT 
ACTAACCTGT CACTGGATAG ATCTGGTTCT CCTATGGTAC CTTCATATGA GACATCTGTC 
AGTCCCCAGG CTAACCGAAC ATATGTTAGG ACAGAGACCA CTGAGGATGA ACGCAAAATT 
CTTCTGGACA GTGTGCAGTT AAAAGACCTG TGGAAAAAAA TCTGCCATCA CAGCAGTGGA 
ATGGAGTTTC AGGATCACCG CTACTGGTTG AGAACGCATC CCAACTGCAT TGTAGGAAAG 
GAATTAGTCA ACTGGCTAAT CCGAAATGGG CATATTGCCA CAAGGGCACA AGCTATAGCA 
ATTGGACAAG CAATGGTTGA TGGACGTTGG CTGGATTGTG TTAGTCATCA CGACCAGCTT 
TTCAGAGATG AGTATGCGCT GTATAGACCA CTGCAGAGTA CAGAATTTTC TGAGACGCCT 
TCTCCCGACA GTGACTCAGT GAACTCCGTG GAAGGACACT CTGAGCCATC CTGGTTTAAA 
GACATAAAGT TTGATGACAG TGACACAGAA CAGATAGCTG AAGAAGGTGA CGATAATTTG 
GCTAAGTATT TGATTTCTGA CACTGGAGGA CAACAGCTCT CAATAAGTGA CGCTTTCATC 
AAAGAATCCT TATTTAATCG CCGAGTAGAG GAAAAATCCA AAGAGCTGCC TTTCACACCT 
TTGGGCTGGC ATCATAACAA CCTGGAGCTC CTGAGGGAGG AGAATGGGGA GAAACAAGCC 
ATGGAGAGGT TGCTTTCAGC TAATCATAAC CACATGATGG CACTACTCCA GCAGTTGCTC 
CATAGTGACT CACTGTCATC ATCTTGGAGG GACATCATCG TGTCATTGGT CTGCCAGGTT 
GTTCAGACAG TCCGACCTGA TGTCAAGAAC CAGGATGATG ACATGGATAT CCGTCAGTTT 
GTCCACATCA AAAAAATCCC AGGTGGAAAG AAGTTTGATT CTGTGGTTGT CAATGGCTTT 
GTTTGTACCA AGAACATTGC ACATAAAAAG ATGAGTTCTT GTATTAAAAA CCCCAAAATT 
CTTCTGTTGA AGTGTTCCAT TGAGTATCTC TACAGAGAAG AAACTAAGTT TACTTGCATT 
GATCCTATTG TGCTTCAGGA AAGGGAATTC TTGAAGAATT ATGTCCAGCG AATAGTTGAT 
GTTCGACCCA CCTTGGTTCT TGTTGAGAAA ACAGTGTCTC GGATTGCCCA GGACATGTTA 
TTGGAACATG GCATTACTTT GGTCATTAAT GTAAAGTCAC AAGTTTTGGA ACGAATCAGT 
CGAATGACCC AAGGTGATTT AGTGATGTCA ATGGACCAGC TGCTTACGAA ACCACACCTG 
GGCACTTGTC ACAAATTTTA TATGCAGATA TTTCAGTTGC CTAATGAACA AACCAAGACA 
CTGATGTTTT TTGAAGGTTG TCCACAGCAC CTAGGCTGTA CAATCAAGCT AAGAGGAGGC 
TCTGATTATG AGCTGGCTCG AGTTAAGGAG ATCCTAATAT TTATGATCTG TGTTGCTTAT 
CATTCTCAAC TAGAAATATC CTTTCTCATG GATGAATTTG CTATGCCTCC CACATTAATG 
CAAAACCCTT CATTCCATTC CCTGATTGAG GGACGAGGGC ATGAGGGGGC TGTCCAAGAG 
CAGTACGGTG GAGGTTCCAT CCCCTGGGAT CCTGACATCC CTCCTGAGTC TCTGCCCTGT 
GATGATAGCA GTTTGCTGGA ATTGAGGATT GTGTTTGAGA AGGGTGAGCA GGAAAATAAA 
AATCTTCCGC AGGCTGTTGC CTCTGTGAAG CATCAAGAAC ATAGCACAAC AGCTTGCCCG 
GCGGGTCTCC CTTGTGCTTT CTTTGCACCT GTACCGGAAT CATTGTTGCC ACTCCCTGTG 
GATGACCAAC AAGATGCTTT AGGCAGCGAG CAGCCAGAGA CTTTGCAGCA AACAGTTGTG 
CTGCAGGATC CCAAAAGCCA GATAAGAGCC TTTAGAGACC CTCTACAGGA TGACACTGGA 
TTATATGTTA CTGAGGAAGT CACCTCCTCT GAAGATAAAC GAAAGACTTA TTCTTTGGCC 
TTTAAGCAGG AATTAAAAGA TGTGATCCTC TGTATCTCCC CAGTAATCAC ATTCCGAGAA 
CCCTTTCTTT TAACTGAAAA GGGGATGAGA TGCTCTACCC GAGATTATTT TGCAGAGCAG 
GTTTACTGGT CTCCTCTCCT CAATAAAGAA TTCAAAGAAA TGGAGAACAG GAGGAAGAAA 
CAGCTGCTCA GGGATCTCTC TGGACTTCAG GGCATGAATG GAAGTATTCA GGCCAAGTCT 
ATTCAAGTCT TACCCTCACA TGAGCTAGTG AGCACTAGAA TTGCTGAGCA TCTGGGCGAT 
AGCCAGAGCT TGGGTAGAAT GCTGGCCGAT TATCGAGCCA GAGGAGGAAG AATTCAGCCC 
AAAAATTCAG ACCCTTTTGC TCATTCAAAG GATGCATCAA GTACTTCAAG TGGCCAATCA 
GGAAGCAAAA ATGAGGGTGA TGAAGAGAGA GGGCTTATTC TGAGTGATGC TGTGTGGTCA 
ACAAAGGTGG ACTGTCTGAA TCCCATTAAT CACCAGAGAC TTTGTGTGCT CTTCAGCAGC 
TCTTCTGCCC AGTCCAGCAA TGCTCCTAGT GCCTGTGTCA GTCCTTGGAT TGTAACAATG 
GAATTTTATG GAAAGAATGA TCTTACATTA GGAATATTTT TAGAGAGATA CTGTTTCAGG 
CCTTCTTATC AGTGTCCAAG CATGTTCTGT GATACCCCCA TGGTACATCA TATTCGGCGC 
TTTGTTCATG GCCAAGGCTG TGTGCAGATA ATCCTGAAGG AGTTGGATTC TCCAGTACCT 
GGATATCAGC ATACAATTCT TACATATTCC TGGTGTAGAA TCTGCAAACA GGTAACACCA 
GTTGTTGCTC TTTCCAATGA GTCCTGGTCT ATGTCATTTG CAAAATACCT TGAACTTAGG 
TTTTATGGGC ACCAGTATAC TCGCAGAGCC AACGCTGAGC CCTGTGGTCA CTCCATCCAT 
CATGATTATC ACCAGTATTT CTCCTATAAC CAGATGGTGG CGTCTTTCAG TTATTCTCCC 
ATTCGGCTTC TTGAAGTATG TGTTCCACTC CCCAAAATAT TCATTAAGCG TCAGGCCCCA 
TTAAAAGTGT CCCTTCTTCA GGATCTGAAG GACTTCTTTC AAAAAGTTTC ACAGGTATAT 
GTTGCCATTG ATGAAAGACT TGCATCTTTG AAAACTGATA CATTTAGTAA AACAAGAGAG 
GAAAAAATGG AAGATATTTT TGCACAGAAA GAGATGGAAG AAGGTGAGTT CAAGAACTGG 
ATTGAGAAGA TGCAAGCAAG GCTCATGTCT TCCTCTGTAG ATACCCCTCA GCAACTGCAG 
TCGGTCTTTG AGTCACTCAT TGCCAAGAAA CAAAGTCTCT GTGAAGTGCT GCAAGCTTGG 
AATAACAGGT TGCAGGACCT TTTCCAACAG GAAAAGGGTA GAAAGAGACC TTCAGTTCCT 
CCAAGTCCTG GAAGACTGAG ACAAGGGGAA GAAAGCAAGA TAAGTGCGAT GGATGCATCT 
CCACGGAATA TTTCTCCAGG ACTTCAGAAT GGAGAAAAAG AGGATCGCTT CTTAACAACT 
TTGTCCAGCC AGAGCTCCAC CAGTTCTACT CATCTCCAAT TGCCTACGCC ACCTGAAGTC 
ATGTCTGAAC AGTCAGTGGG AGGGCCCCCT GAGCTAGATA CAGCCAGCAG TTCCGAAGAT 
GTGTTTGATG GGCATTTGCT GGGATCCACA GACAGCCAAG TGAAGGAAAA GTCAACCATG 
AAAGCCATCT TTGCAAATTT GCTTCCAGGA AATAGCTATA ATCCTATTCC ATTTCCTTTT 
GATCCAGATA AACACTACTT AATGTATGAA CATGAACGAG TGCCCATTGC AGTCTGCGAG 
AAGGAACCCA GCTCCATCAT TGCTTTTGCT CTCAGTTGTA AAGAATACCG AAATGCCTTA 
GAGGAATTGT CTAAAGCGAC TCAGTGGAAC AGTGCCGAAG AAGGGCTTCC AACAAATAGT 
ACTTCAGATA GCAGACCAAA GAGTAGCAGC CCTATCAGAT TACCTGAAAT GAGTGGAGGA 
CAGACAAATC GTACAACAGA AACAGAACCA CAACCAACCA AAAAGGCTTC TGGAATGTTG 
TCCTTCTTCA GAGGGACAGC AGGGAAAAGC CCCGATCTCT CTTCCCAGAA GAGAGAGACC 
TTACGTGGAG CAGATAGTGC TTACTACCAG GTTGGGCAGA CGGGCAAGGA GGGGACCGAG 
AATCAAGGCG TTGAGCCTCA AGATGAAGTA GATGGAGGAG ATACACAAAA GAAGCAACTC 
ATAAATCCTC ATGTGGAACT TCAATTTTCA GATGCTAATG CCAAGTTTTA CTGTCGGCTC 
TACTATGCGG GAGAGTTTCA TAAGATGCGT GAAGTGATTC TGGACAGCAG TGAAGAAGAT 
TTCATTCGTT CCCTCTCCCA CTCATCACCC TGGCAGGCCC GGGGAGGCAA ATCAGGAGCT 
GCCTTCTATG CAACTGAGGA TGATAGATTT ATTTTGAAGC AAATGCCTCG TCTGGAAGTC 
CAGTCCTTCC TCGACTTTGC ACCACATTAC TTCAATTATA TTACAAATGC TGTTCAACAA 
AAGAGGCCCA CGGCGTTGGC CAAAATTCTT GGAGTTTACA GAATTGGTTA TAAGAACTCT 
CAGAACAACA CTGAGAAGAA GTTAGATCTC CTTGTCATGG AAAATCTTTT CTACGGGAGA 
AAGATGGCAC AGGTTTTTGA TTTGAAGGGC TCTCTTAGGA ATCGGAATGT AAAAACTGAC 
ACTGGAAAAG AGAGTTGTGA TGTGGTCCTG CTAGATGAAA ATCTCCTAAA GATGGTTCGA 
GACAACCCTC TATATATTCG TTCTCATTCC AAAGCTGTGC TGAGAACCTC GATCCATAGT 
GACTCCCATT TCCTTTCTAG CCACCTCATT ATAGATTATT CTTTGCTGGT TGGGCGAGAT 
GATACTAGCA ATGAGCTAGT AGTTGGAATT ATAGATTATA TTCGAACATT TACATGGGAC 
AAAAAGCTTG AGATGGTTGT GAAATCAACA GGAATTTTAG GTGGACAAGG TAAAATGCCA 
ACAGTGGTGT CTCCGGAGTT GTACAGGACT AGGTTTTGTG AGGCAATGGA CAAGTATTTC 
CTAATGGTAC CAGACCACTG GACAGGCTTG GGTCTGAATT GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PIKFYVE ( XM_011510784.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011509086.1 Homo sapiens phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X8, mRNA.

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CloneID	OHu77666
Clone ID Related Accession (Same CDS sequence)	XM_011510784.2
Accession Version	XM_011510784.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6162bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	1-phosphatidylinositol 3-phosphate 5-kinase isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011510784.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG ATGATAAGAC GTCCCCAACA CTGGACTCTG CTAATGATTT GCCTCGATCT 
CCTACTAGTC CTTCTCATCT CACACACTTT AAACCTTTGA CTCCTGATCA AGATGAGCCC 
CCTTTTAAAT CAGCTTATAG TTCTTTTGTA AATCTCTTTC GTTTTAACAA AGAGAGAGCA 
GAAGGAGGCC AGGGAGAACA GCAGCCTTTG AGTGGAAGTT GGACCAGCCC TCAGCTCCCT 
TCGAGGACAC AGTCTGTTAG GTCACCCACA CCTTATAAAA AGCAGCTTAA TGAGGAACTC 
CAGCGGCGCT CTTCAGCATT AGACAACAGT TTGCAACATC CCCAGGAGAA CACAGACACA 
AGAAGGAAAG CAGAACCTAC CTTTGGAGGT CATGACCCTC GTACAGCTGT TCAGCTTCGA 
AGCCTCAGCA CAGTATTAAA ACGCCTCAAG GAAATCATGG AGGGGAAAAG CCAGGATAGT 
GACCTGAAAC AATACTGGAT GCCAGATAGC CAATGTAAAG AGTGCTATGA CTGTAGTGAG 
AAATTTACAA CCTTTAGGCG CAGACACCAT TGCCGACTAT GTGGGCAGAT TTTCTGCAGT 
CGTTGCTGTA ATCAAGAAAT CCCTGGAAAA TTTATGGGCT ATACAGGAGA CCTCCGAGCT 
TGCACATATT GTAGAAAAAT AGCCTTAAGT TATGCTCATT CCACAGACAG TAATTCTATT 
GGGGAAGACT TGAATGCTCT TTCAGATTCT GCTTGCTCTG TGTCTGTGCT TGATCCAAGT 
GAACCCCGAA CACCTGTTGG GAGTAGGAAA GCCAGCCGTA ACATATTTTT AGAGGATGAT 
TTGGCCTGGC AAAGTTTGAT TCATCCAGAT TCCTCAAATA CTCCTCTTTC AACAAGACTT 
GTATCTGTGC AAGAGGATGC TGGGAAATCT CCTGCTCGAA ATAGATCAGC CAGCATTACT 
AACCTGTCAC TGGATAGATC TGGTTCTCCT ATGGTACCTT CATATGAGAC ATCTGTCAGT 
CCCCAGGCTA ACCGAACATA TGTTAGGACA GAGACCACTG AGGATGAACG CAAAATTCTT 
CTGGACAGTG TGCAGTTAAA AGACCTGTGG AAAAAAATCT GCCATCACAG CAGTGGAATG 
GAGTTTCAGG ATCACCGCTA CTGGTTGAGA ACGCATCCCA ACTGCATTGT AGGAAAGGAA 
TTAGTCAACT GGCTAATCCG AAATGGGCAT ATTGCCACAA GGGCACAAGC TATAGCAATT 
GGACAAGCAA TGGTTGATGG ACGTTGGCTG GATTGTGTTA GTCATCACGA CCAGCTTTTC 
AGAGATGAGT ATGCGCTGTA TAGACCACTG CAGAGTACAG AATTTTCTGA GACGCCTTCT 
CCCGACAGTG ACTCAGTGAA CTCCGTGGAA GGACACTCTG AGCCATCCTG GTTTAAAGAC 
ATAAAGTTTG ATGACAGTGA CACAGAACAG ATAGCTGAAG AAGGTGACGA TAATTTGGCT 
AAGTATTTGA TTTCTGACAC TGGAGGACAA CAGCTCTCAA TAAGTGACGC TTTCATCAAA 
GAATCCTTAT TTAATCGCCG AGTAGAGGAA AAATCCAAAG AGCTGCCTTT CACACCTTTG 
GGCTGGCATC ATAACAACCT GGAGCTCCTG AGGGAGGAGA ATGGGGAGAA ACAAGCCATG 
GAGAGGTTGC TTTCAGCTAA TCATAACCAC ATGATGGCAC TACTCCAGCA GTTGCTCCAT 
AGTGACTCAC TGTCATCATC TTGGAGGGAC ATCATCGTGT CATTGGTCTG CCAGGTTGTT 
CAGACAGTCC GACCTGATGT CAAGAACCAG GATGATGACA TGGATATCCG TCAGTTTGTC 
CACATCAAAA AAATCCCAGG TGGAAAGAAG TTTGATTCTG TGGTTGTCAA TGGCTTTGTT 
TGTACCAAGA ACATTGCACA TAAAAAGATG AGTTCTTGTA TTAAAAACCC CAAAATTCTT 
CTGTTGAAGT GTTCCATTGA GTATCTCTAC AGAGAAGAAA CTAAGTTTAC TTGCATTGAT 
CCTATTGTGC TTCAGGAAAG GGAATTCTTG AAGAATTATG TCCAGCGAAT AGTTGATGTT 
CGACCCACCT TGGTTCTTGT TGAGAAAACA GTGTCTCGGA TTGCCCAGGA CATGTTATTG 
GAACATGGCA TTACTTTGGT CATTAATGTA AAGTCACAAG TTTTGGAACG AATCAGTCGA 
ATGACCCAAG GTGATTTAGT GATGTCAATG GACCAGCTGC TTACGAAACC ACACCTGGGC 
ACTTGTCACA AATTTTATAT GCAGATATTT CAGTTGCCTA ATGAACAAAC CAAGACACTG 
ATGTTTTTTG AAGGTTGTCC ACAGCACCTA GGCTGTACAA TCAAGCTAAG AGGAGGCTCT 
GATTATGAGC TGGCTCGAGT TAAGGAGATC CTAATATTTA TGATCTGTGT TGCTTATCAT 
TCTCAACTAG AAATATCCTT TCTCATGGAT GAATTTGCTA TGCCTCCCAC ATTAATGCAA 
AACCCTTCAT TCCATTCCCT GATTGAGGGA CGAGGGCATG AGGGGGCTGT CCAAGAGCAG 
TACGGTGGAG GTTCCATCCC CTGGGATCCT GACATCCCTC CTGAGTCTCT GCCCTGTGAT 
GATAGCAGTT TGCTGGAATT GAGGATTGTG TTTGAGAAGG GTGAGCAGGA AAATAAAAAT 
CTTCCGCAGG CTGTTGCCTC TGTGAAGCAT CAAGAACATA GCACAACAGC TTGCCCGGCG 
GGTCTCCCTT GTGCTTTCTT TGCACCTGTA CCGGAATCAT TGTTGCCACT CCCTGTGGAT 
GACCAACAAG ATGCTTTAGG CAGCGAGCAG CCAGAGACTT TGCAGCAAAC AGTTGTGCTG 
CAGGATCCCA AAAGCCAGAT AAGAGCCTTT AGAGACCCTC TACAGGATGA CACTGGATTA 
TATGTTACTG AGGAAGTCAC CTCCTCTGAA GATAAACGAA AGACTTATTC TTTGGCCTTT 
AAGCAGGAAT TAAAAGATGT GATCCTCTGT ATCTCCCCAG TAATCACATT CCGAGAACCC 
TTTCTTTTAA CTGAAAAGGG GATGAGATGC TCTACCCGAG ATTATTTTGC AGAGCAGGTT 
TACTGGTCTC CTCTCCTCAA TAAAGAATTC AAAGAAATGG AGAACAGGAG GAAGAAACAG 
CTGCTCAGGG ATCTCTCTGG ACTTCAGGGC ATGAATGGAA GTATTCAGGC CAAGTCTATT 
CAAGTCTTAC CCTCACATGA GCTAGTGAGC ACTAGAATTG CTGAGCATCT GGGCGATAGC 
CAGAGCTTGG GTAGAATGCT GGCCGATTAT CGAGCCAGAG GAGGAAGAAT TCAGCCCAAA 
AATTCAGACC CTTTTGCTCA TTCAAAGGAT GCATCAAGTA CTTCAAGTGG CCAATCAGGA 
AGCAAAAATG AGGGTGATGA AGAGAGAGGG CTTATTCTGA GTGATGCTGT GTGGTCAACA 
AAGGTGGACT GTCTGAATCC CATTAATCAC CAGAGACTTT GTGTGCTCTT CAGCAGCTCT 
TCTGCCCAGT CCAGCAATGC TCCTAGTGCC TGTGTCAGTC CTTGGATTGT AACAATGGAA 
TTTTATGGAA AGAATGATCT TACATTAGGA ATATTTTTAG AGAGATACTG TTTCAGGCCT 
TCTTATCAGT GTCCAAGCAT GTTCTGTGAT ACCCCCATGG TACATCATAT TCGGCGCTTT 
GTTCATGGCC AAGGCTGTGT GCAGATAATC CTGAAGGAGT TGGATTCTCC AGTACCTGGA 
TATCAGCATA CAATTCTTAC ATATTCCTGG TGTAGAATCT GCAAACAGGT AACACCAGTT 
GTTGCTCTTT CCAATGAGTC CTGGTCTATG TCATTTGCAA AATACCTTGA ACTTAGGTTT 
TATGGGCACC AGTATACTCG CAGAGCCAAC GCTGAGCCCT GTGGTCACTC CATCCATCAT 
GATTATCACC AGTATTTCTC CTATAACCAG ATGGTGGCGT CTTTCAGTTA TTCTCCCATT 
CGGCTTCTTG AAGTATGTGT TCCACTCCCC AAAATATTCA TTAAGCGTCA GGCCCCATTA 
AAAGTGTCCC TTCTTCAGGA TCTGAAGGAC TTCTTTCAAA AAGTTTCACA GGTATATGTT 
GCCATTGATG AAAGACTTGC ATCTTTGAAA ACTGATACAT TTAGTAAAAC AAGAGAGGAA 
AAAATGGAAG ATATTTTTGC ACAGAAAGAG ATGGAAGAAG GTGAGTTCAA GAACTGGATT 
GAGAAGATGC AAGCAAGGCT CATGTCTTCC TCTGTAGATA CCCCTCAGCA ACTGCAGTCG 
GTCTTTGAGT CACTCATTGC CAAGAAACAA AGTCTCTGTG AAGTGCTGCA AGCTTGGAAT 
AACAGGTTGC AGGACCTTTT CCAACAGGAA AAGGGTAGAA AGAGACCTTC AGTTCCTCCA 
AGTCCTGGAA GACTGAGACA AGGGGAAGAA AGCAAGATAA GTGCGATGGA TGCATCTCCA 
CGGAATATTT CTCCAGGACT TCAGAATGGA GAAAAAGAGG ATCGCTTCTT AACAACTTTG 
TCCAGCCAGA GCTCCACCAG TTCTACTCAT CTCCAATTGC CTACGCCACC TGAAGTCATG 
TCTGAACAGT CAGTGGGAGG GCCCCCTGAG CTAGATACAG CCAGCAGTTC CGAAGATGTG 
TTTGATGGGC ATTTGCTGGG ATCCACAGAC AGCCAAGTGA AGGAAAAGTC AACCATGAAA 
GCCATCTTTG CAAATTTGCT TCCAGGAAAT AGCTATAATC CTATTCCATT TCCTTTTGAT 
CCAGATAAAC ACTACTTAAT GTATGAACAT GAACGAGTGC CCATTGCAGT CTGCGAGAAG 
GAACCCAGCT CCATCATTGC TTTTGCTCTC AGTTGTAAAG AATACCGAAA TGCCTTAGAG 
GAATTGTCTA AAGCGACTCA GTGGAACAGT GCCGAAGAAG GGCTTCCAAC AAATAGTACT 
TCAGATAGCA GACCAAAGAG TAGCAGCCCT ATCAGATTAC CTGAAATGAG TGGAGGACAG 
ACAAATCGTA CAACAGAAAC AGAACCACAA CCAACCAAAA AGGCTTCTGG AATGTTGTCC 
TTCTTCAGAG GGACAGCAGG GAAAAGCCCC GATCTCTCTT CCCAGAAGAG AGAGACCTTA 
CGTGGAGCAG ATAGTGCTTA CTACCAGGTT GGGCAGACGG GCAAGGAGGG GACCGAGAAT 
CAAGGCGTTG AGCCTCAAGA TGAAGTAGAT GGAGGAGATA CACAAAAGAA GCAACTCATA 
AATCCTCATG TGGAACTTCA ATTTTCAGAT GCTAATGCCA AGTTTTACTG TCGGCTCTAC 
TATGCGGGAG AGTTTCATAA GATGCGTGAA GTGATTCTGG ACAGCAGTGA AGAAGATTTC 
ATTCGTTCCC TCTCCCACTC ATCACCCTGG CAGGCCCGGG GAGGCAAATC AGGAGCTGCC 
TTCTATGCAA CTGAGGATGA TAGATTTATT TTGAAGCAAA TGCCTCGTCT GGAAGTCCAG 
TCCTTCCTCG ACTTTGCACC ACATTACTTC AATTATATTA CAAATGCTGT TCAACAAAAG 
AGGCCCACGG CGTTGGCCAA AATTCTTGGA GTTTACAGAA TTGGTTATAA GAACTCTCAG 
AACAACACTG AGAAGAAGTT AGATCTCCTT GTCATGGAAA ATCTTTTCTA CGGGAGAAAG 
ATGGCACAGG TTTTTGATTT GAAGGGCTCT CTTAGGAATC GGAATGTAAA AACTGACACT 
GGAAAAGAGA GTTGTGATGT GGTCCTGCTA GATGAAAATC TCCTAAAGAT GGTTCGAGAC 
AACCCTCTAT ATATTCGTTC TCATTCCAAA GCTGTGCTGA GAACCTCGAT CCATAGTGAC 
TCCCATTTCC TTTCTAGCCA CCTCATTATA GATTATTCTT TGCTGGTTGG GCGAGATGAT 
ACTAGCAATG AGCTAGTAGT TGGAATTATA GATTATATTC GAACATTTAC ATGGGACAAA 
AAGCTTGAGA TGGTTGTGAA ATCAACAGGA ATTTTAGGTG GACAAGGTAA AATGCCAACA 
GTGGTGTCTC CGGAGTTGTA CAGGACTAGG TTTTGTGAGG CAATGGACAA GTATTTCCTA 
ATGGTACCAG ACCACTGGAC AGGCTTGGGT CTGAATTGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011509086.1
CDS	194..6355
Translation	MATDDKTSPTLDSANDLPRSPTSPSHLTHFKPLTPDQDEPPFKSAYSSFVNLFRFNKERAEGGQGEQQPLSGSWTSPQLPSRTQSVRSPTPYKKQLNEELQRRSSALDNSLQHPQENTDTRRKAEPTFGGHDPRTAVQLRSLSTVLKRLKEIMEGKSQDSDLKQYWMPDSQCKECYDCSEKFTTFRRRHHCRLCGQIFCSRCCNQEIPGKFMGYTGDLRACTYCRKIALSYAHSTDSNSIGEDLNALSDSACSVSVLDPSEPRTPVGSRKASRNIFLEDDLAWQSLIHPDSSNTPLSTRLVSVQEDAGKSPARNRSASITNLSLDRSGSPMVPSYETSVSPQANRTYVRTETTEDERKILLDSVQLKDLWKKICHHSSGMEFQDHRYWLRTHPNCIVGKELVNWLIRNGHIATRAQAIAIGQAMVDGRWLDCVSHHDQLFRDEYALYRPLQSTEFSETPSPDSDSVNSVEGHSEPSWFKDIKFDDSDTEQIAEEGDDNLAKYLISDTGGQQLSISDAFIKESLFNRRVEEKSKELPFTPLGWHHNNLELLREENGEKQAMERLLSANHNHMMALLQQLLHSDSLSSSWRDIIVSLVCQVVQTVRPDVKNQDDDMDIRQFVHIKKIPGGKKFDSVVVNGFVCTKNIAHKKMSSCIKNPKILLLKCSIEYLYREETKFTCIDPIVLQEREFLKNYVQRIVDVRPTLVLVEKTVSRIAQDMLLEHGITLVINVKSQVLERISRMTQGDLVMSMDQLLTKPHLGTCHKFYMQIFQLPNEQTKTLMFFEGCPQHLGCTIKLRGGSDYELARVKEILIFMICVAYHSQLEISFLMDEFAMPPTLMQNPSFHSLIEGRGHEGAVQEQYGGGSIPWDPDIPPESLPCDDSSLLELRIVFEKGEQENKNLPQAVASVKHQEHSTTACPAGLPCAFFAPVPESLLPLPVDDQQDALGSEQPETLQQTVVLQDPKSQIRAFRDPLQDDTGLYVTEEVTSSEDKRKTYSLAFKQELKDVILCISPVITFREPFLLTEKGMRCSTRDYFAEQVYWSPLLNKEFKEMENRRKKQLLRDLSGLQGMNGSIQAKSIQVLPSHELVSTRIAEHLGDSQSLGRMLADYRARGGRIQPKNSDPFAHSKDASSTSSGQSGSKNEGDEERGLILSDAVWSTKVDCLNPINHQRLCVLFSSSSAQSSNAPSACVSPWIVTMEFYGKNDLTLGIFLERYCFRPSYQCPSMFCDTPMVHHIRRFVHGQGCVQIILKELDSPVPGYQHTILTYSWCRICKQVTPVVALSNESWSMSFAKYLELRFYGHQYTRRANAEPCGHSIHHDYHQYFSYNQMVASFSYSPIRLLEVCVPLPKIFIKRQAPLKVSLLQDLKDFFQKVSQVYVAIDERLASLKTDTFSKTREEKMEDIFAQKEMEEGEFKNWIEKMQARLMSSSVDTPQQLQSVFESLIAKKQSLCEVLQAWNNRLQDLFQQEKGRKRPSVPPSPGRLRQGEESKISAMDASPRNISPGLQNGEKEDRFLTTLSSQSSTSSTHLQLPTPPEVMSEQSVGGPPELDTASSSEDVFDGHLLGSTDSQVKEKSTMKAIFANLLPGNSYNPIPFPFDPDKHYLMYEHERVPIAVCEKEPSSIIAFALSCKEYRNALEELSKATQWNSAEEGLPTNSTSDSRPKSSSPIRLPEMSGGQTNRTTETEPQPTKKASGMLSFFRGTAGKSPDLSSQKRETLRGADSAYYQVGQTGKEGTENQGVEPQDEVDGGDTQKKQLINPHVELQFSDANAKFYCRLYYAGEFHKMREVILDSSEEDFIRSLSHSSPWQARGGKSGAAFYATEDDRFILKQMPRLEVQSFLDFAPHYFNYITNAVQQKRPTALAKILGVYRIGYKNSQNNTEKKLDLLVMENLFYGRKMAQVFDLKGSLRNRNVKTDTGKESCDVVLLDENLLKMVRDNPLYIRSHSKAVLRTSIHSDSHFLSSHLIIDYSLLVGRDDTSNELVVGIIDYIRTFTWDKKLEMVVKSTGILGGQGKMPTVVSPELYRTRFCEAMDKYFLMVPDHWTGLGLNC

Target ORF information:

RefSeq Version	XM_011510784.2
Organism	Homo sapiens(human)
Definition	Homo sapiens phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011510784.2

ORF Insert Sequence:

ATGGCCACAG ATGATAAGAC GTCCCCAACA CTGGACTCTG CTAATGATTT GCCTCGATCT 
CCTACTAGTC CTTCTCATCT CACACACTTT AAACCTTTGA CTCCTGATCA AGATGAGCCC 
CCTTTTAAAT CAGCTTATAG TTCTTTTGTA AATCTCTTTC GTTTTAACAA AGAGAGAGCA 
GAAGGAGGCC AGGGAGAACA GCAGCCTTTG AGTGGAAGTT GGACCAGCCC TCAGCTCCCT 
TCGAGGACAC AGTCTGTTAG GTCACCCACA CCTTATAAAA AGCAGCTTAA TGAGGAACTC 
CAGCGGCGCT CTTCAGCATT AGACAACAGT TTGCAACATC CCCAGGAGAA CACAGACACA 
AGAAGGAAAG CAGAACCTAC CTTTGGAGGT CATGACCCTC GTACAGCTGT TCAGCTTCGA 
AGCCTCAGCA CAGTATTAAA ACGCCTCAAG GAAATCATGG AGGGGAAAAG CCAGGATAGT 
GACCTGAAAC AATACTGGAT GCCAGATAGC CAATGTAAAG AGTGCTATGA CTGTAGTGAG 
AAATTTACAA CCTTTAGGCG CAGACACCAT TGCCGACTAT GTGGGCAGAT TTTCTGCAGT 
CGTTGCTGTA ATCAAGAAAT CCCTGGAAAA TTTATGGGCT ATACAGGAGA CCTCCGAGCT 
TGCACATATT GTAGAAAAAT AGCCTTAAGT TATGCTCATT CCACAGACAG TAATTCTATT 
GGGGAAGACT TGAATGCTCT TTCAGATTCT GCTTGCTCTG TGTCTGTGCT TGATCCAAGT 
GAACCCCGAA CACCTGTTGG GAGTAGGAAA GCCAGCCGTA ACATATTTTT AGAGGATGAT 
TTGGCCTGGC AAAGTTTGAT TCATCCAGAT TCCTCAAATA CTCCTCTTTC AACAAGACTT 
GTATCTGTGC AAGAGGATGC TGGGAAATCT CCTGCTCGAA ATAGATCAGC CAGCATTACT 
AACCTGTCAC TGGATAGATC TGGTTCTCCT ATGGTACCTT CATATGAGAC ATCTGTCAGT 
CCCCAGGCTA ACCGAACATA TGTTAGGACA GAGACCACTG AGGATGAACG CAAAATTCTT 
CTGGACAGTG TGCAGTTAAA AGACCTGTGG AAAAAAATCT GCCATCACAG CAGTGGAATG 
GAGTTTCAGG ATCACCGCTA CTGGTTGAGA ACGCATCCCA ACTGCATTGT AGGAAAGGAA 
TTAGTCAACT GGCTAATCCG AAATGGGCAT ATTGCCACAA GGGCACAAGC TATAGCAATT 
GGACAAGCAA TGGTTGATGG ACGTTGGCTG GATTGTGTTA GTCATCACGA CCAGCTTTTC 
AGAGATGAGT ATGCGCTGTA TAGACCACTG CAGAGTACAG AATTTTCTGA GACGCCTTCT 
CCCGACAGTG ACTCAGTGAA CTCCGTGGAA GGACACTCTG AGCCATCCTG GTTTAAAGAC 
ATAAAGTTTG ATGACAGTGA CACAGAACAG ATAGCTGAAG AAGGTGACGA TAATTTGGCT 
AAGTATTTGA TTTCTGACAC TGGAGGACAA CAGCTCTCAA TAAGTGACGC TTTCATCAAA 
GAATCCTTAT TTAATCGCCG AGTAGAGGAA AAATCCAAAG AGCTGCCTTT CACACCTTTG 
GGCTGGCATC ATAACAACCT GGAGCTCCTG AGGGAGGAGA ATGGGGAGAA ACAAGCCATG 
GAGAGGTTGC TTTCAGCTAA TCATAACCAC ATGATGGCAC TACTCCAGCA GTTGCTCCAT 
AGTGACTCAC TGTCATCATC TTGGAGGGAC ATCATCGTGT CATTGGTCTG CCAGGTTGTT 
CAGACAGTCC GACCTGATGT CAAGAACCAG GATGATGACA TGGATATCCG TCAGTTTGTC 
CACATCAAAA AAATCCCAGG TGGAAAGAAG TTTGATTCTG TGGTTGTCAA TGGCTTTGTT 
TGTACCAAGA ACATTGCACA TAAAAAGATG AGTTCTTGTA TTAAAAACCC CAAAATTCTT 
CTGTTGAAGT GTTCCATTGA GTATCTCTAC AGAGAAGAAA CTAAGTTTAC TTGCATTGAT 
CCTATTGTGC TTCAGGAAAG GGAATTCTTG AAGAATTATG TCCAGCGAAT AGTTGATGTT 
CGACCCACCT TGGTTCTTGT TGAGAAAACA GTGTCTCGGA TTGCCCAGGA CATGTTATTG 
GAACATGGCA TTACTTTGGT CATTAATGTA AAGTCACAAG TTTTGGAACG AATCAGTCGA 
ATGACCCAAG GTGATTTAGT GATGTCAATG GACCAGCTGC TTACGAAACC ACACCTGGGC 
ACTTGTCACA AATTTTATAT GCAGATATTT CAGTTGCCTA ATGAACAAAC CAAGACACTG 
ATGTTTTTTG AAGGTTGTCC ACAGCACCTA GGCTGTACAA TCAAGCTAAG AGGAGGCTCT 
GATTATGAGC TGGCTCGAGT TAAGGAGATC CTAATATTTA TGATCTGTGT TGCTTATCAT 
TCTCAACTAG AAATATCCTT TCTCATGGAT GAATTTGCTA TGCCTCCCAC ATTAATGCAA 
AACCCTTCAT TCCATTCCCT GATTGAGGGA CGAGGGCATG AGGGGGCTGT CCAAGAGCAG 
TACGGTGGAG GTTCCATCCC CTGGGATCCT GACATCCCTC CTGAGTCTCT GCCCTGTGAT 
GATAGCAGTT TGCTGGAATT GAGGATTGTG TTTGAGAAGG GTGAGCAGGA AAATAAAAAT 
CTTCCGCAGG CTGTTGCCTC TGTGAAGCAT CAAGAACATA GCACAACAGC TTGCCCGGCG 
GGTCTCCCTT GTGCTTTCTT TGCACCTGTA CCGGAATCAT TGTTGCCACT CCCTGTGGAT 
GACCAACAAG ATGCTTTAGG CAGCGAGCAG CCAGAGACTT TGCAGCAAAC AGTTGTGCTG 
CAGGATCCCA AAAGCCAGAT AAGAGCCTTT AGAGACCCTC TACAGGATGA CACTGGATTA 
TATGTTACTG AGGAAGTCAC CTCCTCTGAA GATAAACGAA AGACTTATTC TTTGGCCTTT 
AAGCAGGAAT TAAAAGATGT GATCCTCTGT ATCTCCCCAG TAATCACATT CCGAGAACCC 
TTTCTTTTAA CTGAAAAGGG GATGAGATGC TCTACCCGAG ATTATTTTGC AGAGCAGGTT 
TACTGGTCTC CTCTCCTCAA TAAAGAATTC AAAGAAATGG AGAACAGGAG GAAGAAACAG 
CTGCTCAGGG ATCTCTCTGG ACTTCAGGGC ATGAATGGAA GTATTCAGGC CAAGTCTATT 
CAAGTCTTAC CCTCACATGA GCTAGTGAGC ACTAGAATTG CTGAGCATCT GGGCGATAGC 
CAGAGCTTGG GTAGAATGCT GGCCGATTAT CGAGCCAGAG GAGGAAGAAT TCAGCCCAAA 
AATTCAGACC CTTTTGCTCA TTCAAAGGAT GCATCAAGTA CTTCAAGTGG CCAATCAGGA 
AGCAAAAATG AGGGTGATGA AGAGAGAGGG CTTATTCTGA GTGATGCTGT GTGGTCAACA 
AAGGTGGACT GTCTGAATCC CATTAATCAC CAGAGACTTT GTGTGCTCTT CAGCAGCTCT 
TCTGCCCAGT CCAGCAATGC TCCTAGTGCC TGTGTCAGTC CTTGGATTGT AACAATGGAA 
TTTTATGGAA AGAATGATCT TACATTAGGA ATATTTTTAG AGAGATACTG TTTCAGGCCT 
TCTTATCAGT GTCCAAGCAT GTTCTGTGAT ACCCCCATGG TACATCATAT TCGGCGCTTT 
GTTCATGGCC AAGGCTGTGT GCAGATAATC CTGAAGGAGT TGGATTCTCC AGTACCTGGA 
TATCAGCATA CAATTCTTAC ATATTCCTGG TGTAGAATCT GCAAACAGGT AACACCAGTT 
GTTGCTCTTT CCAATGAGTC CTGGTCTATG TCATTTGCAA AATACCTTGA ACTTAGGTTT 
TATGGGCACC AGTATACTCG CAGAGCCAAC GCTGAGCCCT GTGGTCACTC CATCCATCAT 
GATTATCACC AGTATTTCTC CTATAACCAG ATGGTGGCGT CTTTCAGTTA TTCTCCCATT 
CGGCTTCTTG AAGTATGTGT TCCACTCCCC AAAATATTCA TTAAGCGTCA GGCCCCATTA 
AAAGTGTCCC TTCTTCAGGA TCTGAAGGAC TTCTTTCAAA AAGTTTCACA GGTATATGTT 
GCCATTGATG AAAGACTTGC ATCTTTGAAA ACTGATACAT TTAGTAAAAC AAGAGAGGAA 
AAAATGGAAG ATATTTTTGC ACAGAAAGAG ATGGAAGAAG GTGAGTTCAA GAACTGGATT 
GAGAAGATGC AAGCAAGGCT CATGTCTTCC TCTGTAGATA CCCCTCAGCA ACTGCAGTCG 
GTCTTTGAGT CACTCATTGC CAAGAAACAA AGTCTCTGTG AAGTGCTGCA AGCTTGGAAT 
AACAGGTTGC AGGACCTTTT CCAACAGGAA AAGGGTAGAA AGAGACCTTC AGTTCCTCCA 
AGTCCTGGAA GACTGAGACA AGGGGAAGAA AGCAAGATAA GTGCGATGGA TGCATCTCCA 
CGGAATATTT CTCCAGGACT TCAGAATGGA GAAAAAGAGG ATCGCTTCTT AACAACTTTG 
TCCAGCCAGA GCTCCACCAG TTCTACTCAT CTCCAATTGC CTACGCCACC TGAAGTCATG 
TCTGAACAGT CAGTGGGAGG GCCCCCTGAG CTAGATACAG CCAGCAGTTC CGAAGATGTG 
TTTGATGGGC ATTTGCTGGG ATCCACAGAC AGCCAAGTGA AGGAAAAGTC AACCATGAAA 
GCCATCTTTG CAAATTTGCT TCCAGGAAAT AGCTATAATC CTATTCCATT TCCTTTTGAT 
CCAGATAAAC ACTACTTAAT GTATGAACAT GAACGAGTGC CCATTGCAGT CTGCGAGAAG 
GAACCCAGCT CCATCATTGC TTTTGCTCTC AGTTGTAAAG AATACCGAAA TGCCTTAGAG 
GAATTGTCTA AAGCGACTCA GTGGAACAGT GCCGAAGAAG GGCTTCCAAC AAATAGTACT 
TCAGATAGCA GACCAAAGAG TAGCAGCCCT ATCAGATTAC CTGAAATGAG TGGAGGACAG 
ACAAATCGTA CAACAGAAAC AGAACCACAA CCAACCAAAA AGGCTTCTGG AATGTTGTCC 
TTCTTCAGAG GGACAGCAGG GAAAAGCCCC GATCTCTCTT CCCAGAAGAG AGAGACCTTA 
CGTGGAGCAG ATAGTGCTTA CTACCAGGTT GGGCAGACGG GCAAGGAGGG GACCGAGAAT 
CAAGGCGTTG AGCCTCAAGA TGAAGTAGAT GGAGGAGATA CACAAAAGAA GCAACTCATA 
AATCCTCATG TGGAACTTCA ATTTTCAGAT GCTAATGCCA AGTTTTACTG TCGGCTCTAC 
TATGCGGGAG AGTTTCATAA GATGCGTGAA GTGATTCTGG ACAGCAGTGA AGAAGATTTC 
ATTCGTTCCC TCTCCCACTC ATCACCCTGG CAGGCCCGGG GAGGCAAATC AGGAGCTGCC 
TTCTATGCAA CTGAGGATGA TAGATTTATT TTGAAGCAAA TGCCTCGTCT GGAAGTCCAG 
TCCTTCCTCG ACTTTGCACC ACATTACTTC AATTATATTA CAAATGCTGT TCAACAAAAG 
AGGCCCACGG CGTTGGCCAA AATTCTTGGA GTTTACAGAA TTGGTTATAA GAACTCTCAG 
AACAACACTG AGAAGAAGTT AGATCTCCTT GTCATGGAAA ATCTTTTCTA CGGGAGAAAG 
ATGGCACAGG TTTTTGATTT GAAGGGCTCT CTTAGGAATC GGAATGTAAA AACTGACACT 
GGAAAAGAGA GTTGTGATGT GGTCCTGCTA GATGAAAATC TCCTAAAGAT GGTTCGAGAC 
AACCCTCTAT ATATTCGTTC TCATTCCAAA GCTGTGCTGA GAACCTCGAT CCATAGTGAC 
TCCCATTTCC TTTCTAGCCA CCTCATTATA GATTATTCTT TGCTGGTTGG GCGAGATGAT 
ACTAGCAATG AGCTAGTAGT TGGAATTATA GATTATATTC GAACATTTAC ATGGGACAAA 
AAGCTTGAGA TGGTTGTGAA ATCAACAGGA ATTTTAGGTG GACAAGGTAA AATGCCAACA 
GTGGTGTCTC CGGAGTTGTA CAGGACTAGG TTTTGTGAGG CAATGGACAA GTATTTCCTA 
ATGGTACCAG ACCACTGGAC AGGCTTGGGT CTGAATTGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PIKFYVE ( XM_011510785.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011509087.1 Homo sapiens phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X9, mRNA.

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CloneID	OHu77667
Clone ID Related Accession (Same CDS sequence)	XM_011510785.3 , XM_011510785.2
Accession Version	XM_011510785.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6147bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	1-phosphatidylinositol 3-phosphate 5-kinase isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011510785.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG ATGATAAGAC GTCCCCAACA CTGGACTCTG CTAATGATTT GCCTCGATCT 
CCTACTAGTC CTTCTCATCT CACACACTTT AAACCTTTGA CTCCTGATCA AGATGAGCCC 
CCTTTTAAAT CAGCTTATAG TTCTTTTGTA AATCTCTTTC GTTTTAACAA AGAGAGAGCA 
GAAGGAGGCC AGGGAGAACA GCAGCCTTTG AGTGGAAGTT GGACCAGCCC TCAGCTCCCT 
TCGAGGACAC AGTCTGTTAG GTCACCCACA CCTTATAAAA AGCAGCTTAA TGAGGAACTC 
CAGCGGCGCT CTTCAGCATT AGCAGACAAC AGTTTGCAAC ATCCCCAGGA GAACACAGAC 
ACAAGAAGGA AAGCAGAACC TACCTTTGGA GGTCATGACC CTCGTACAGC TGTTCAGCTT 
CGAAGCCTCA GCACAGTATT AAAACGCCTC AAGGAAATCA TGGAGGGGAA AAGCCAGGAT 
AGTGACCTGA AACAATACTG GATGCCAGAT AGCCAATGTA AAGAGTGCTA TGACTGTAGT 
GAGAAATTTA CAACCTTTAG GCGCAGACAC CATTGCCGAC TATGTGGGCA GATTTTCTGC 
AGTCGTTGCT GTAATCAAGA AATCCCTGGA AAATTTATGG GCTATACAGG AGACCTCCGA 
GCTTGCACAT ATTGTAGAAA AATAGCCTTA AGTTATGCTC ATTCCACAGA CAGTAATTCT 
ATTGGGGAAG ACTTGAATGC TCTTTCAGAT TCTGCTTGCT CTGTGTCTGT GCTTGATCCA 
AGTGAACCCC GAACACCTGT TGGGAGTAGG AAAGCCAGCC GTAACATATT TTTAGAGGAT 
GATTTGGCCT GGCAAAGTTT GATTCATCCA GATTCCTCAA ATACTCCTCT TTCAACAAGA 
CTTGTATCTG TGCAAGAGGA TGCTGGGAAA TCTCCTGCTC GAAATAGATC AGCCAGCATT 
ACTAACCTGT CACTGGATAG ATCTGGTTCT CCTATGGTAC CTTCATATGA GACATCTGTC 
AGTCCCCAGG CTAACCGAAC ATATGTTAGG ACAGAGACCA CTGAGGATGA ACGCAAAATT 
CTTCTGGACA GTGTGCAGTT AAAAGACCTG TGGAAAAAAA TCTGCCATCA CAGCAGTGGA 
ATGGAGTTTC AGGATCACCG CTACTGGTTG AGAACGCATC CCAACTGCAT TGTAGGAAAG 
GAATTAGTCA ACTGGCTAAT CCGAAATGGG CATATTGCCA CAAGGGCACA AGCTATAGCA 
ATTGGACAAG CAATGGTTGA TGGACGTTGG CTGGATTGTG TTAGTCATCA CGACCAGCTT 
TTCAGAGATG AGTATGCGCT GTATAGACCA CTGCAGAGTA CAGAATTTTC TGAGACGCCT 
TCTCCCGACA GTGACTCAGT GAACTCCGTG GAAGGACACT CTGAGCCATC CTGGTTTAAA 
GACATAAAGT TTGATGACAG TGACACAGAA CAGATAGCTG AAGAAGAGTA TTTGATTTCT 
GACACTGGAG GACAACAGCT CTCAATAAGT GACGCTTTCA TCAAAGAATC CTTATTTAAT 
CGCCGAGTAG AGGAAAAATC CAAAGAGCTG CCTTTCACAC CTTTGGGCTG GCATCATAAC 
AACCTGGAGC TCCTGAGGGA GGAGAATGGG GAGAAACAAG CCATGGAGAG GTTGCTTTCA 
GCTAATCATA ACCACATGAT GGCACTACTC CAGCAGTTGC TCCATAGTGA CTCACTGTCA 
TCATCTTGGA GGGACATCAT CGTGTCATTG GTCTGCCAGG TTGTTCAGAC AGTCCGACCT 
GATGTCAAGA ACCAGGATGA TGACATGGAT ATCCGTCAGT TTGTCCACAT CAAAAAAATC 
CCAGGTGGAA AGAAGTTTGA TTCTGTGGTT GTCAATGGCT TTGTTTGTAC CAAGAACATT 
GCACATAAAA AGATGAGTTC TTGTATTAAA AACCCCAAAA TTCTTCTGTT GAAGTGTTCC 
ATTGAGTATC TCTACAGAGA AGAAACTAAG TTTACTTGCA TTGATCCTAT TGTGCTTCAG 
GAAAGGGAAT TCTTGAAGAA TTATGTCCAG CGAATAGTTG ATGTTCGACC CACCTTGGTT 
CTTGTTGAGA AAACAGTGTC TCGGATTGCC CAGGACATGT TATTGGAACA TGGCATTACT 
TTGGTCATTA ATGTAAAGTC ACAAGTTTTG GAACGAATCA GTCGAATGAC CCAAGGTGAT 
TTAGTGATGT CAATGGACCA GCTGCTTACG AAACCACACC TGGGCACTTG TCACAAATTT 
TATATGCAGA TATTTCAGTT GCCTAATGAA CAAACCAAGA CACTGATGTT TTTTGAAGGT 
TGTCCACAGC ACCTAGGCTG TACAATCAAG CTAAGAGGAG GCTCTGATTA TGAGCTGGCT 
CGAGTTAAGG AGATCCTAAT ATTTATGATC TGTGTTGCTT ATCATTCTCA ACTAGAAATA 
TCCTTTCTCA TGGATGAATT TGCTATGCCT CCCACATTAA TGCAAAACCC TTCATTCCAT 
TCCCTGATTG AGGGACGAGG GCATGAGGGG GCTGTCCAAG AGCAGTACGG TGGAGGTTCC 
ATCCCCTGGG ATCCTGACAT CCCTCCTGAG TCTCTGCCCT GTGATGATAG CAGTTTGCTG 
GAATTGAGGA TTGTGTTTGA GAAGGGTGAG CAGGAAAATA AAAATCTTCC GCAGGCTGTT 
GCCTCTGTGA AGCATCAAGA ACATAGCACA ACAGCTTGCC CGGCGGGTCT CCCTTGTGCT 
TTCTTTGCAC CTGTACCGGA ATCATTGTTG CCACTCCCTG TGGATGACCA ACAAGATGCT 
TTAGGCAGCG AGCAGCCAGA GACTTTGCAG CAAACAGTTG TGCTGCAGGA TCCCAAAAGC 
CAGATAAGAG CCTTTAGAGA CCCTCTACAG GATGACACTG GATTATATGT TACTGAGGAA 
GTCACCTCCT CTGAAGATAA ACGAAAGACT TATTCTTTGG CCTTTAAGCA GGAATTAAAA 
GATGTGATCC TCTGTATCTC CCCAGTAATC ACATTCCGAG AACCCTTTCT TTTAACTGAA 
AAGGGGATGA GATGCTCTAC CCGAGATTAT TTTGCAGAGC AGGTTTACTG GTCTCCTCTC 
CTCAATAAAG AATTCAAAGA AATGGAGAAC AGGAGGAAGA AACAGCTGCT CAGGGATCTC 
TCTGGACTTC AGGGCATGAA TGGAAGTATT CAGGCCAAGT CTATTCAAGT CTTACCCTCA 
CATGAGCTAG TGAGCACTAG AATTGCTGAG CATCTGGGCG ATAGCCAGAG CTTGGGTAGA 
ATGCTGGCCG ATTATCGAGC CAGAGGAGGA AGAATTCAGC CCAAAAATTC AGACCCTTTT 
GCTCATTCAA AGGATGCATC AAGTACTTCA AGTGGCCAAT CAGGAAGCAA AAATGAGGGT 
GATGAAGAGA GAGGGCTTAT TCTGAGTGAT GCTGTGTGGT CAACAAAGGT GGACTGTCTG 
AATCCCATTA ATCACCAGAG ACTTTGTGTG CTCTTCAGCA GCTCTTCTGC CCAGTCCAGC 
AATGCTCCTA GTGCCTGTGT CAGTCCTTGG ATTGTAACAA TGGAATTTTA TGGAAAGAAT 
GATCTTACAT TAGGAATATT TTTAGAGAGA TACTGTTTCA GGCCTTCTTA TCAGTGTCCA 
AGCATGTTCT GTGATACCCC CATGGTACAT CATATTCGGC GCTTTGTTCA TGGCCAAGGC 
TGTGTGCAGA TAATCCTGAA GGAGTTGGAT TCTCCAGTAC CTGGATATCA GCATACAATT 
CTTACATATT CCTGGTGTAG AATCTGCAAA CAGGTAACAC CAGTTGTTGC TCTTTCCAAT 
GAGTCCTGGT CTATGTCATT TGCAAAATAC CTTGAACTTA GGTTTTATGG GCACCAGTAT 
ACTCGCAGAG CCAACGCTGA GCCCTGTGGT CACTCCATCC ATCATGATTA TCACCAGTAT 
TTCTCCTATA ACCAGATGGT GGCGTCTTTC AGTTATTCTC CCATTCGGCT TCTTGAAGTA 
TGTGTTCCAC TCCCCAAAAT ATTCATTAAG CGTCAGGCCC CATTAAAAGT GTCCCTTCTT 
CAGGATCTGA AGGACTTCTT TCAAAAAGTT TCACAGGTAT ATGTTGCCAT TGATGAAAGA 
CTTGCATCTT TGAAAACTGA TACATTTAGT AAAACAAGAG AGGAAAAAAT GGAAGATATT 
TTTGCACAGA AAGAGATGGA AGAAGGTGAG TTCAAGAACT GGATTGAGAA GATGCAAGCA 
AGGCTCATGT CTTCCTCTGT AGATACCCCT CAGCAACTGC AGTCGGTCTT TGAGTCACTC 
ATTGCCAAGA AACAAAGTCT CTGTGAAGTG CTGCAAGCTT GGAATAACAG GTTGCAGGAC 
CTTTTCCAAC AGGAAAAGGG TAGAAAGAGA CCTTCAGTTC CTCCAAGTCC TGGAAGACTG 
AGACAAGGGG AAGAAAGCAA GATAAGTGCG ATGGATGCAT CTCCACGGAA TATTTCTCCA 
GGACTTCAGA ATGGAGAAAA AGAGGATCGC TTCTTAACAA CTTTGTCCAG CCAGAGCTCC 
ACCAGTTCTA CTCATCTCCA ATTGCCTACG CCACCTGAAG TCATGTCTGA ACAGTCAGTG 
GGAGGGCCCC CTGAGCTAGA TACAGCCAGC AGTTCCGAAG ATGTGTTTGA TGGGCATTTG 
CTGGGATCCA CAGACAGCCA AGTGAAGGAA AAGTCAACCA TGAAAGCCAT CTTTGCAAAT 
TTGCTTCCAG GAAATAGCTA TAATCCTATT CCATTTCCTT TTGATCCAGA TAAACACTAC 
TTAATGTATG AACATGAACG AGTGCCCATT GCAGTCTGCG AGAAGGAACC CAGCTCCATC 
ATTGCTTTTG CTCTCAGTTG TAAAGAATAC CGAAATGCCT TAGAGGAATT GTCTAAAGCG 
ACTCAGTGGA ACAGTGCCGA AGAAGGGCTT CCAACAAATA GTACTTCAGA TAGCAGACCA 
AAGAGTAGCA GCCCTATCAG ATTACCTGAA ATGAGTGGAG GACAGACAAA TCGTACAACA 
GAAACAGAAC CACAACCAAC CAAAAAGGCT TCTGGAATGT TGTCCTTCTT CAGAGGGACA 
GCAGGGAAAA GCCCCGATCT CTCTTCCCAG AAGAGAGAGA CCTTACGTGG AGCAGATAGT 
GCTTACTACC AGGTTGGGCA GACGGGCAAG GAGGGGACCG AGAATCAAGG CGTTGAGCCT 
CAAGATGAAG TAGATGGAGG AGATACACAA AAGAAGCAAC TCATAAATCC TCATGTGGAA 
CTTCAATTTT CAGATGCTAA TGCCAAGTTT TACTGTCGGC TCTACTATGC GGGAGAGTTT 
CATAAGATGC GTGAAGTGAT TCTGGACAGC AGTGAAGAAG ATTTCATTCG TTCCCTCTCC 
CACTCATCAC CCTGGCAGGC CCGGGGAGGC AAATCAGGAG CTGCCTTCTA TGCAACTGAG 
GATGATAGAT TTATTTTGAA GCAAATGCCT CGTCTGGAAG TCCAGTCCTT CCTCGACTTT 
GCACCACATT ACTTCAATTA TATTACAAAT GCTGTTCAAC AAAAGAGGCC CACGGCGTTG 
GCCAAAATTC TTGGAGTTTA CAGAATTGGT TATAAGAACT CTCAGAACAA CACTGAGAAG 
AAGTTAGATC TCCTTGTCAT GGAAAATCTT TTCTACGGGA GAAAGATGGC ACAGGTTTTT 
GATTTGAAGG GCTCTCTTAG GAATCGGAAT GTAAAAACTG ACACTGGAAA AGAGAGTTGT 
GATGTGGTCC TGCTAGATGA AAATCTCCTA AAGATGGTTC GAGACAACCC TCTATATATT 
CGTTCTCATT CCAAAGCTGT GCTGAGAACC TCGATCCATA GTGACTCCCA TTTCCTTTCT 
AGCCACCTCA TTATAGATTA TTCTTTGCTG GTTGGGCGAG ATGATACTAG CAATGAGCTA 
GTAGTTGGAA TTATAGATTA TATTCGAACA TTTACATGGG ACAAAAAGCT TGAGATGGTT 
GTGAAATCAA CAGGAATTTT AGGTGGACAA GGTAAAATGC CAACAGTGGT GTCTCCGGAG 
TTGTACAGGA CTAGGTTTTG TGAGGCAATG GACAAGTATT TCCTAATGGT ACCAGACCAC 
TGGACAGGCT TGGGTCTGAA TTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011509087.1
CDS	182..6328
Translation	MATDDKTSPTLDSANDLPRSPTSPSHLTHFKPLTPDQDEPPFKSAYSSFVNLFRFNKERAEGGQGEQQPLSGSWTSPQLPSRTQSVRSPTPYKKQLNEELQRRSSALADNSLQHPQENTDTRRKAEPTFGGHDPRTAVQLRSLSTVLKRLKEIMEGKSQDSDLKQYWMPDSQCKECYDCSEKFTTFRRRHHCRLCGQIFCSRCCNQEIPGKFMGYTGDLRACTYCRKIALSYAHSTDSNSIGEDLNALSDSACSVSVLDPSEPRTPVGSRKASRNIFLEDDLAWQSLIHPDSSNTPLSTRLVSVQEDAGKSPARNRSASITNLSLDRSGSPMVPSYETSVSPQANRTYVRTETTEDERKILLDSVQLKDLWKKICHHSSGMEFQDHRYWLRTHPNCIVGKELVNWLIRNGHIATRAQAIAIGQAMVDGRWLDCVSHHDQLFRDEYALYRPLQSTEFSETPSPDSDSVNSVEGHSEPSWFKDIKFDDSDTEQIAEEEYLISDTGGQQLSISDAFIKESLFNRRVEEKSKELPFTPLGWHHNNLELLREENGEKQAMERLLSANHNHMMALLQQLLHSDSLSSSWRDIIVSLVCQVVQTVRPDVKNQDDDMDIRQFVHIKKIPGGKKFDSVVVNGFVCTKNIAHKKMSSCIKNPKILLLKCSIEYLYREETKFTCIDPIVLQEREFLKNYVQRIVDVRPTLVLVEKTVSRIAQDMLLEHGITLVINVKSQVLERISRMTQGDLVMSMDQLLTKPHLGTCHKFYMQIFQLPNEQTKTLMFFEGCPQHLGCTIKLRGGSDYELARVKEILIFMICVAYHSQLEISFLMDEFAMPPTLMQNPSFHSLIEGRGHEGAVQEQYGGGSIPWDPDIPPESLPCDDSSLLELRIVFEKGEQENKNLPQAVASVKHQEHSTTACPAGLPCAFFAPVPESLLPLPVDDQQDALGSEQPETLQQTVVLQDPKSQIRAFRDPLQDDTGLYVTEEVTSSEDKRKTYSLAFKQELKDVILCISPVITFREPFLLTEKGMRCSTRDYFAEQVYWSPLLNKEFKEMENRRKKQLLRDLSGLQGMNGSIQAKSIQVLPSHELVSTRIAEHLGDSQSLGRMLADYRARGGRIQPKNSDPFAHSKDASSTSSGQSGSKNEGDEERGLILSDAVWSTKVDCLNPINHQRLCVLFSSSSAQSSNAPSACVSPWIVTMEFYGKNDLTLGIFLERYCFRPSYQCPSMFCDTPMVHHIRRFVHGQGCVQIILKELDSPVPGYQHTILTYSWCRICKQVTPVVALSNESWSMSFAKYLELRFYGHQYTRRANAEPCGHSIHHDYHQYFSYNQMVASFSYSPIRLLEVCVPLPKIFIKRQAPLKVSLLQDLKDFFQKVSQVYVAIDERLASLKTDTFSKTREEKMEDIFAQKEMEEGEFKNWIEKMQARLMSSSVDTPQQLQSVFESLIAKKQSLCEVLQAWNNRLQDLFQQEKGRKRPSVPPSPGRLRQGEESKISAMDASPRNISPGLQNGEKEDRFLTTLSSQSSTSSTHLQLPTPPEVMSEQSVGGPPELDTASSSEDVFDGHLLGSTDSQVKEKSTMKAIFANLLPGNSYNPIPFPFDPDKHYLMYEHERVPIAVCEKEPSSIIAFALSCKEYRNALEELSKATQWNSAEEGLPTNSTSDSRPKSSSPIRLPEMSGGQTNRTTETEPQPTKKASGMLSFFRGTAGKSPDLSSQKRETLRGADSAYYQVGQTGKEGTENQGVEPQDEVDGGDTQKKQLINPHVELQFSDANAKFYCRLYYAGEFHKMREVILDSSEEDFIRSLSHSSPWQARGGKSGAAFYATEDDRFILKQMPRLEVQSFLDFAPHYFNYITNAVQQKRPTALAKILGVYRIGYKNSQNNTEKKLDLLVMENLFYGRKMAQVFDLKGSLRNRNVKTDTGKESCDVVLLDENLLKMVRDNPLYIRSHSKAVLRTSIHSDSHFLSSHLIIDYSLLVGRDDTSNELVVGIIDYIRTFTWDKKLEMVVKSTGILGGQGKMPTVVSPELYRTRFCEAMDKYFLMVPDHWTGLGLNC

Target ORF information:

RefSeq Version	XM_011510785.3
Organism	Homo sapiens(human)
Definition	Homo sapiens phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011510785.3

ORF Insert Sequence:

ATGGCCACAG ATGATAAGAC GTCCCCAACA CTGGACTCTG CTAATGATTT GCCTCGATCT 
CCTACTAGTC CTTCTCATCT CACACACTTT AAACCTTTGA CTCCTGATCA AGATGAGCCC 
CCTTTTAAAT CAGCTTATAG TTCTTTTGTA AATCTCTTTC GTTTTAACAA AGAGAGAGCA 
GAAGGAGGCC AGGGAGAACA GCAGCCTTTG AGTGGAAGTT GGACCAGCCC TCAGCTCCCT 
TCGAGGACAC AGTCTGTTAG GTCACCCACA CCTTATAAAA AGCAGCTTAA TGAGGAACTC 
CAGCGGCGCT CTTCAGCATT AGCAGACAAC AGTTTGCAAC ATCCCCAGGA GAACACAGAC 
ACAAGAAGGA AAGCAGAACC TACCTTTGGA GGTCATGACC CTCGTACAGC TGTTCAGCTT 
CGAAGCCTCA GCACAGTATT AAAACGCCTC AAGGAAATCA TGGAGGGGAA AAGCCAGGAT 
AGTGACCTGA AACAATACTG GATGCCAGAT AGCCAATGTA AAGAGTGCTA TGACTGTAGT 
GAGAAATTTA CAACCTTTAG GCGCAGACAC CATTGCCGAC TATGTGGGCA GATTTTCTGC 
AGTCGTTGCT GTAATCAAGA AATCCCTGGA AAATTTATGG GCTATACAGG AGACCTCCGA 
GCTTGCACAT ATTGTAGAAA AATAGCCTTA AGTTATGCTC ATTCCACAGA CAGTAATTCT 
ATTGGGGAAG ACTTGAATGC TCTTTCAGAT TCTGCTTGCT CTGTGTCTGT GCTTGATCCA 
AGTGAACCCC GAACACCTGT TGGGAGTAGG AAAGCCAGCC GTAACATATT TTTAGAGGAT 
GATTTGGCCT GGCAAAGTTT GATTCATCCA GATTCCTCAA ATACTCCTCT TTCAACAAGA 
CTTGTATCTG TGCAAGAGGA TGCTGGGAAA TCTCCTGCTC GAAATAGATC AGCCAGCATT 
ACTAACCTGT CACTGGATAG ATCTGGTTCT CCTATGGTAC CTTCATATGA GACATCTGTC 
AGTCCCCAGG CTAACCGAAC ATATGTTAGG ACAGAGACCA CTGAGGATGA ACGCAAAATT 
CTTCTGGACA GTGTGCAGTT AAAAGACCTG TGGAAAAAAA TCTGCCATCA CAGCAGTGGA 
ATGGAGTTTC AGGATCACCG CTACTGGTTG AGAACGCATC CCAACTGCAT TGTAGGAAAG 
GAATTAGTCA ACTGGCTAAT CCGAAATGGG CATATTGCCA CAAGGGCACA AGCTATAGCA 
ATTGGACAAG CAATGGTTGA TGGACGTTGG CTGGATTGTG TTAGTCATCA CGACCAGCTT 
TTCAGAGATG AGTATGCGCT GTATAGACCA CTGCAGAGTA CAGAATTTTC TGAGACGCCT 
TCTCCCGACA GTGACTCAGT GAACTCCGTG GAAGGACACT CTGAGCCATC CTGGTTTAAA 
GACATAAAGT TTGATGACAG TGACACAGAA CAGATAGCTG AAGAAGAGTA TTTGATTTCT 
GACACTGGAG GACAACAGCT CTCAATAAGT GACGCTTTCA TCAAAGAATC CTTATTTAAT 
CGCCGAGTAG AGGAAAAATC CAAAGAGCTG CCTTTCACAC CTTTGGGCTG GCATCATAAC 
AACCTGGAGC TCCTGAGGGA GGAGAATGGG GAGAAACAAG CCATGGAGAG GTTGCTTTCA 
GCTAATCATA ACCACATGAT GGCACTACTC CAGCAGTTGC TCCATAGTGA CTCACTGTCA 
TCATCTTGGA GGGACATCAT CGTGTCATTG GTCTGCCAGG TTGTTCAGAC AGTCCGACCT 
GATGTCAAGA ACCAGGATGA TGACATGGAT ATCCGTCAGT TTGTCCACAT CAAAAAAATC 
CCAGGTGGAA AGAAGTTTGA TTCTGTGGTT GTCAATGGCT TTGTTTGTAC CAAGAACATT 
GCACATAAAA AGATGAGTTC TTGTATTAAA AACCCCAAAA TTCTTCTGTT GAAGTGTTCC 
ATTGAGTATC TCTACAGAGA AGAAACTAAG TTTACTTGCA TTGATCCTAT TGTGCTTCAG 
GAAAGGGAAT TCTTGAAGAA TTATGTCCAG CGAATAGTTG ATGTTCGACC CACCTTGGTT 
CTTGTTGAGA AAACAGTGTC TCGGATTGCC CAGGACATGT TATTGGAACA TGGCATTACT 
TTGGTCATTA ATGTAAAGTC ACAAGTTTTG GAACGAATCA GTCGAATGAC CCAAGGTGAT 
TTAGTGATGT CAATGGACCA GCTGCTTACG AAACCACACC TGGGCACTTG TCACAAATTT 
TATATGCAGA TATTTCAGTT GCCTAATGAA CAAACCAAGA CACTGATGTT TTTTGAAGGT 
TGTCCACAGC ACCTAGGCTG TACAATCAAG CTAAGAGGAG GCTCTGATTA TGAGCTGGCT 
CGAGTTAAGG AGATCCTAAT ATTTATGATC TGTGTTGCTT ATCATTCTCA ACTAGAAATA 
TCCTTTCTCA TGGATGAATT TGCTATGCCT CCCACATTAA TGCAAAACCC TTCATTCCAT 
TCCCTGATTG AGGGACGAGG GCATGAGGGG GCTGTCCAAG AGCAGTACGG TGGAGGTTCC 
ATCCCCTGGG ATCCTGACAT CCCTCCTGAG TCTCTGCCCT GTGATGATAG CAGTTTGCTG 
GAATTGAGGA TTGTGTTTGA GAAGGGTGAG CAGGAAAATA AAAATCTTCC GCAGGCTGTT 
GCCTCTGTGA AGCATCAAGA ACATAGCACA ACAGCTTGCC CGGCGGGTCT CCCTTGTGCT 
TTCTTTGCAC CTGTACCGGA ATCATTGTTG CCACTCCCTG TGGATGACCA ACAAGATGCT 
TTAGGCAGCG AGCAGCCAGA GACTTTGCAG CAAACAGTTG TGCTGCAGGA TCCCAAAAGC 
CAGATAAGAG CCTTTAGAGA CCCTCTACAG GATGACACTG GATTATATGT TACTGAGGAA 
GTCACCTCCT CTGAAGATAA ACGAAAGACT TATTCTTTGG CCTTTAAGCA GGAATTAAAA 
GATGTGATCC TCTGTATCTC CCCAGTAATC ACATTCCGAG AACCCTTTCT TTTAACTGAA 
AAGGGGATGA GATGCTCTAC CCGAGATTAT TTTGCAGAGC AGGTTTACTG GTCTCCTCTC 
CTCAATAAAG AATTCAAAGA AATGGAGAAC AGGAGGAAGA AACAGCTGCT CAGGGATCTC 
TCTGGACTTC AGGGCATGAA TGGAAGTATT CAGGCCAAGT CTATTCAAGT CTTACCCTCA 
CATGAGCTAG TGAGCACTAG AATTGCTGAG CATCTGGGCG ATAGCCAGAG CTTGGGTAGA 
ATGCTGGCCG ATTATCGAGC CAGAGGAGGA AGAATTCAGC CCAAAAATTC AGACCCTTTT 
GCTCATTCAA AGGATGCATC AAGTACTTCA AGTGGCCAAT CAGGAAGCAA AAATGAGGGT 
GATGAAGAGA GAGGGCTTAT TCTGAGTGAT GCTGTGTGGT CAACAAAGGT GGACTGTCTG 
AATCCCATTA ATCACCAGAG ACTTTGTGTG CTCTTCAGCA GCTCTTCTGC CCAGTCCAGC 
AATGCTCCTA GTGCCTGTGT CAGTCCTTGG ATTGTAACAA TGGAATTTTA TGGAAAGAAT 
GATCTTACAT TAGGAATATT TTTAGAGAGA TACTGTTTCA GGCCTTCTTA TCAGTGTCCA 
AGCATGTTCT GTGATACCCC CATGGTACAT CATATTCGGC GCTTTGTTCA TGGCCAAGGC 
TGTGTGCAGA TAATCCTGAA GGAGTTGGAT TCTCCAGTAC CTGGATATCA GCATACAATT 
CTTACATATT CCTGGTGTAG AATCTGCAAA CAGGTAACAC CAGTTGTTGC TCTTTCCAAT 
GAGTCCTGGT CTATGTCATT TGCAAAATAC CTTGAACTTA GGTTTTATGG GCACCAGTAT 
ACTCGCAGAG CCAACGCTGA GCCCTGTGGT CACTCCATCC ATCATGATTA TCACCAGTAT 
TTCTCCTATA ACCAGATGGT GGCGTCTTTC AGTTATTCTC CCATTCGGCT TCTTGAAGTA 
TGTGTTCCAC TCCCCAAAAT ATTCATTAAG CGTCAGGCCC CATTAAAAGT GTCCCTTCTT 
CAGGATCTGA AGGACTTCTT TCAAAAAGTT TCACAGGTAT ATGTTGCCAT TGATGAAAGA 
CTTGCATCTT TGAAAACTGA TACATTTAGT AAAACAAGAG AGGAAAAAAT GGAAGATATT 
TTTGCACAGA AAGAGATGGA AGAAGGTGAG TTCAAGAACT GGATTGAGAA GATGCAAGCA 
AGGCTCATGT CTTCCTCTGT AGATACCCCT CAGCAACTGC AGTCGGTCTT TGAGTCACTC 
ATTGCCAAGA AACAAAGTCT CTGTGAAGTG CTGCAAGCTT GGAATAACAG GTTGCAGGAC 
CTTTTCCAAC AGGAAAAGGG TAGAAAGAGA CCTTCAGTTC CTCCAAGTCC TGGAAGACTG 
AGACAAGGGG AAGAAAGCAA GATAAGTGCG ATGGATGCAT CTCCACGGAA TATTTCTCCA 
GGACTTCAGA ATGGAGAAAA AGAGGATCGC TTCTTAACAA CTTTGTCCAG CCAGAGCTCC 
ACCAGTTCTA CTCATCTCCA ATTGCCTACG CCACCTGAAG TCATGTCTGA ACAGTCAGTG 
GGAGGGCCCC CTGAGCTAGA TACAGCCAGC AGTTCCGAAG ATGTGTTTGA TGGGCATTTG 
CTGGGATCCA CAGACAGCCA AGTGAAGGAA AAGTCAACCA TGAAAGCCAT CTTTGCAAAT 
TTGCTTCCAG GAAATAGCTA TAATCCTATT CCATTTCCTT TTGATCCAGA TAAACACTAC 
TTAATGTATG AACATGAACG AGTGCCCATT GCAGTCTGCG AGAAGGAACC CAGCTCCATC 
ATTGCTTTTG CTCTCAGTTG TAAAGAATAC CGAAATGCCT TAGAGGAATT GTCTAAAGCG 
ACTCAGTGGA ACAGTGCCGA AGAAGGGCTT CCAACAAATA GTACTTCAGA TAGCAGACCA 
AAGAGTAGCA GCCCTATCAG ATTACCTGAA ATGAGTGGAG GACAGACAAA TCGTACAACA 
GAAACAGAAC CACAACCAAC CAAAAAGGCT TCTGGAATGT TGTCCTTCTT CAGAGGGACA 
GCAGGGAAAA GCCCCGATCT CTCTTCCCAG AAGAGAGAGA CCTTACGTGG AGCAGATAGT 
GCTTACTACC AGGTTGGGCA GACGGGCAAG GAGGGGACCG AGAATCAAGG CGTTGAGCCT 
CAAGATGAAG TAGATGGAGG AGATACACAA AAGAAGCAAC TCATAAATCC TCATGTGGAA 
CTTCAATTTT CAGATGCTAA TGCCAAGTTT TACTGTCGGC TCTACTATGC GGGAGAGTTT 
CATAAGATGC GTGAAGTGAT TCTGGACAGC AGTGAAGAAG ATTTCATTCG TTCCCTCTCC 
CACTCATCAC CCTGGCAGGC CCGGGGAGGC AAATCAGGAG CTGCCTTCTA TGCAACTGAG 
GATGATAGAT TTATTTTGAA GCAAATGCCT CGTCTGGAAG TCCAGTCCTT CCTCGACTTT 
GCACCACATT ACTTCAATTA TATTACAAAT GCTGTTCAAC AAAAGAGGCC CACGGCGTTG 
GCCAAAATTC TTGGAGTTTA CAGAATTGGT TATAAGAACT CTCAGAACAA CACTGAGAAG 
AAGTTAGATC TCCTTGTCAT GGAAAATCTT TTCTACGGGA GAAAGATGGC ACAGGTTTTT 
GATTTGAAGG GCTCTCTTAG GAATCGGAAT GTAAAAACTG ACACTGGAAA AGAGAGTTGT 
GATGTGGTCC TGCTAGATGA AAATCTCCTA AAGATGGTTC GAGACAACCC TCTATATATT 
CGTTCTCATT CCAAAGCTGT GCTGAGAACC TCGATCCATA GTGACTCCCA TTTCCTTTCT 
AGCCACCTCA TTATAGATTA TTCTTTGCTG GTTGGGCGAG ATGATACTAG CAATGAGCTA 
GTAGTTGGAA TTATAGATTA TATTCGAACA TTTACATGGG ACAAAAAGCT TGAGATGGTT 
GTGAAATCAA CAGGAATTTT AGGTGGACAA GGTAAAATGC CAACAGTGGT GTCTCCGGAG 
TTGTACAGGA CTAGGTTTTG TGAGGCAATG GACAAGTATT TCCTAATGGT ACCAGACCAC 
TGGACAGGCT TGGGTCTGAA TTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PIKFYVE ( XM_011510785.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011509087.1 Homo sapiens phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X9, mRNA.

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CloneID	OHu77667
Clone ID Related Accession (Same CDS sequence)	XM_011510785.3 , XM_011510785.2
Accession Version	XM_011510785.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6147bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	1-phosphatidylinositol 3-phosphate 5-kinase isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011510785.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG ATGATAAGAC GTCCCCAACA CTGGACTCTG CTAATGATTT GCCTCGATCT 
CCTACTAGTC CTTCTCATCT CACACACTTT AAACCTTTGA CTCCTGATCA AGATGAGCCC 
CCTTTTAAAT CAGCTTATAG TTCTTTTGTA AATCTCTTTC GTTTTAACAA AGAGAGAGCA 
GAAGGAGGCC AGGGAGAACA GCAGCCTTTG AGTGGAAGTT GGACCAGCCC TCAGCTCCCT 
TCGAGGACAC AGTCTGTTAG GTCACCCACA CCTTATAAAA AGCAGCTTAA TGAGGAACTC 
CAGCGGCGCT CTTCAGCATT AGCAGACAAC AGTTTGCAAC ATCCCCAGGA GAACACAGAC 
ACAAGAAGGA AAGCAGAACC TACCTTTGGA GGTCATGACC CTCGTACAGC TGTTCAGCTT 
CGAAGCCTCA GCACAGTATT AAAACGCCTC AAGGAAATCA TGGAGGGGAA AAGCCAGGAT 
AGTGACCTGA AACAATACTG GATGCCAGAT AGCCAATGTA AAGAGTGCTA TGACTGTAGT 
GAGAAATTTA CAACCTTTAG GCGCAGACAC CATTGCCGAC TATGTGGGCA GATTTTCTGC 
AGTCGTTGCT GTAATCAAGA AATCCCTGGA AAATTTATGG GCTATACAGG AGACCTCCGA 
GCTTGCACAT ATTGTAGAAA AATAGCCTTA AGTTATGCTC ATTCCACAGA CAGTAATTCT 
ATTGGGGAAG ACTTGAATGC TCTTTCAGAT TCTGCTTGCT CTGTGTCTGT GCTTGATCCA 
AGTGAACCCC GAACACCTGT TGGGAGTAGG AAAGCCAGCC GTAACATATT TTTAGAGGAT 
GATTTGGCCT GGCAAAGTTT GATTCATCCA GATTCCTCAA ATACTCCTCT TTCAACAAGA 
CTTGTATCTG TGCAAGAGGA TGCTGGGAAA TCTCCTGCTC GAAATAGATC AGCCAGCATT 
ACTAACCTGT CACTGGATAG ATCTGGTTCT CCTATGGTAC CTTCATATGA GACATCTGTC 
AGTCCCCAGG CTAACCGAAC ATATGTTAGG ACAGAGACCA CTGAGGATGA ACGCAAAATT 
CTTCTGGACA GTGTGCAGTT AAAAGACCTG TGGAAAAAAA TCTGCCATCA CAGCAGTGGA 
ATGGAGTTTC AGGATCACCG CTACTGGTTG AGAACGCATC CCAACTGCAT TGTAGGAAAG 
GAATTAGTCA ACTGGCTAAT CCGAAATGGG CATATTGCCA CAAGGGCACA AGCTATAGCA 
ATTGGACAAG CAATGGTTGA TGGACGTTGG CTGGATTGTG TTAGTCATCA CGACCAGCTT 
TTCAGAGATG AGTATGCGCT GTATAGACCA CTGCAGAGTA CAGAATTTTC TGAGACGCCT 
TCTCCCGACA GTGACTCAGT GAACTCCGTG GAAGGACACT CTGAGCCATC CTGGTTTAAA 
GACATAAAGT TTGATGACAG TGACACAGAA CAGATAGCTG AAGAAGAGTA TTTGATTTCT 
GACACTGGAG GACAACAGCT CTCAATAAGT GACGCTTTCA TCAAAGAATC CTTATTTAAT 
CGCCGAGTAG AGGAAAAATC CAAAGAGCTG CCTTTCACAC CTTTGGGCTG GCATCATAAC 
AACCTGGAGC TCCTGAGGGA GGAGAATGGG GAGAAACAAG CCATGGAGAG GTTGCTTTCA 
GCTAATCATA ACCACATGAT GGCACTACTC CAGCAGTTGC TCCATAGTGA CTCACTGTCA 
TCATCTTGGA GGGACATCAT CGTGTCATTG GTCTGCCAGG TTGTTCAGAC AGTCCGACCT 
GATGTCAAGA ACCAGGATGA TGACATGGAT ATCCGTCAGT TTGTCCACAT CAAAAAAATC 
CCAGGTGGAA AGAAGTTTGA TTCTGTGGTT GTCAATGGCT TTGTTTGTAC CAAGAACATT 
GCACATAAAA AGATGAGTTC TTGTATTAAA AACCCCAAAA TTCTTCTGTT GAAGTGTTCC 
ATTGAGTATC TCTACAGAGA AGAAACTAAG TTTACTTGCA TTGATCCTAT TGTGCTTCAG 
GAAAGGGAAT TCTTGAAGAA TTATGTCCAG CGAATAGTTG ATGTTCGACC CACCTTGGTT 
CTTGTTGAGA AAACAGTGTC TCGGATTGCC CAGGACATGT TATTGGAACA TGGCATTACT 
TTGGTCATTA ATGTAAAGTC ACAAGTTTTG GAACGAATCA GTCGAATGAC CCAAGGTGAT 
TTAGTGATGT CAATGGACCA GCTGCTTACG AAACCACACC TGGGCACTTG TCACAAATTT 
TATATGCAGA TATTTCAGTT GCCTAATGAA CAAACCAAGA CACTGATGTT TTTTGAAGGT 
TGTCCACAGC ACCTAGGCTG TACAATCAAG CTAAGAGGAG GCTCTGATTA TGAGCTGGCT 
CGAGTTAAGG AGATCCTAAT ATTTATGATC TGTGTTGCTT ATCATTCTCA ACTAGAAATA 
TCCTTTCTCA TGGATGAATT TGCTATGCCT CCCACATTAA TGCAAAACCC TTCATTCCAT 
TCCCTGATTG AGGGACGAGG GCATGAGGGG GCTGTCCAAG AGCAGTACGG TGGAGGTTCC 
ATCCCCTGGG ATCCTGACAT CCCTCCTGAG TCTCTGCCCT GTGATGATAG CAGTTTGCTG 
GAATTGAGGA TTGTGTTTGA GAAGGGTGAG CAGGAAAATA AAAATCTTCC GCAGGCTGTT 
GCCTCTGTGA AGCATCAAGA ACATAGCACA ACAGCTTGCC CGGCGGGTCT CCCTTGTGCT 
TTCTTTGCAC CTGTACCGGA ATCATTGTTG CCACTCCCTG TGGATGACCA ACAAGATGCT 
TTAGGCAGCG AGCAGCCAGA GACTTTGCAG CAAACAGTTG TGCTGCAGGA TCCCAAAAGC 
CAGATAAGAG CCTTTAGAGA CCCTCTACAG GATGACACTG GATTATATGT TACTGAGGAA 
GTCACCTCCT CTGAAGATAA ACGAAAGACT TATTCTTTGG CCTTTAAGCA GGAATTAAAA 
GATGTGATCC TCTGTATCTC CCCAGTAATC ACATTCCGAG AACCCTTTCT TTTAACTGAA 
AAGGGGATGA GATGCTCTAC CCGAGATTAT TTTGCAGAGC AGGTTTACTG GTCTCCTCTC 
CTCAATAAAG AATTCAAAGA AATGGAGAAC AGGAGGAAGA AACAGCTGCT CAGGGATCTC 
TCTGGACTTC AGGGCATGAA TGGAAGTATT CAGGCCAAGT CTATTCAAGT CTTACCCTCA 
CATGAGCTAG TGAGCACTAG AATTGCTGAG CATCTGGGCG ATAGCCAGAG CTTGGGTAGA 
ATGCTGGCCG ATTATCGAGC CAGAGGAGGA AGAATTCAGC CCAAAAATTC AGACCCTTTT 
GCTCATTCAA AGGATGCATC AAGTACTTCA AGTGGCCAAT CAGGAAGCAA AAATGAGGGT 
GATGAAGAGA GAGGGCTTAT TCTGAGTGAT GCTGTGTGGT CAACAAAGGT GGACTGTCTG 
AATCCCATTA ATCACCAGAG ACTTTGTGTG CTCTTCAGCA GCTCTTCTGC CCAGTCCAGC 
AATGCTCCTA GTGCCTGTGT CAGTCCTTGG ATTGTAACAA TGGAATTTTA TGGAAAGAAT 
GATCTTACAT TAGGAATATT TTTAGAGAGA TACTGTTTCA GGCCTTCTTA TCAGTGTCCA 
AGCATGTTCT GTGATACCCC CATGGTACAT CATATTCGGC GCTTTGTTCA TGGCCAAGGC 
TGTGTGCAGA TAATCCTGAA GGAGTTGGAT TCTCCAGTAC CTGGATATCA GCATACAATT 
CTTACATATT CCTGGTGTAG AATCTGCAAA CAGGTAACAC CAGTTGTTGC TCTTTCCAAT 
GAGTCCTGGT CTATGTCATT TGCAAAATAC CTTGAACTTA GGTTTTATGG GCACCAGTAT 
ACTCGCAGAG CCAACGCTGA GCCCTGTGGT CACTCCATCC ATCATGATTA TCACCAGTAT 
TTCTCCTATA ACCAGATGGT GGCGTCTTTC AGTTATTCTC CCATTCGGCT TCTTGAAGTA 
TGTGTTCCAC TCCCCAAAAT ATTCATTAAG CGTCAGGCCC CATTAAAAGT GTCCCTTCTT 
CAGGATCTGA AGGACTTCTT TCAAAAAGTT TCACAGGTAT ATGTTGCCAT TGATGAAAGA 
CTTGCATCTT TGAAAACTGA TACATTTAGT AAAACAAGAG AGGAAAAAAT GGAAGATATT 
TTTGCACAGA AAGAGATGGA AGAAGGTGAG TTCAAGAACT GGATTGAGAA GATGCAAGCA 
AGGCTCATGT CTTCCTCTGT AGATACCCCT CAGCAACTGC AGTCGGTCTT TGAGTCACTC 
ATTGCCAAGA AACAAAGTCT CTGTGAAGTG CTGCAAGCTT GGAATAACAG GTTGCAGGAC 
CTTTTCCAAC AGGAAAAGGG TAGAAAGAGA CCTTCAGTTC CTCCAAGTCC TGGAAGACTG 
AGACAAGGGG AAGAAAGCAA GATAAGTGCG ATGGATGCAT CTCCACGGAA TATTTCTCCA 
GGACTTCAGA ATGGAGAAAA AGAGGATCGC TTCTTAACAA CTTTGTCCAG CCAGAGCTCC 
ACCAGTTCTA CTCATCTCCA ATTGCCTACG CCACCTGAAG TCATGTCTGA ACAGTCAGTG 
GGAGGGCCCC CTGAGCTAGA TACAGCCAGC AGTTCCGAAG ATGTGTTTGA TGGGCATTTG 
CTGGGATCCA CAGACAGCCA AGTGAAGGAA AAGTCAACCA TGAAAGCCAT CTTTGCAAAT 
TTGCTTCCAG GAAATAGCTA TAATCCTATT CCATTTCCTT TTGATCCAGA TAAACACTAC 
TTAATGTATG AACATGAACG AGTGCCCATT GCAGTCTGCG AGAAGGAACC CAGCTCCATC 
ATTGCTTTTG CTCTCAGTTG TAAAGAATAC CGAAATGCCT TAGAGGAATT GTCTAAAGCG 
ACTCAGTGGA ACAGTGCCGA AGAAGGGCTT CCAACAAATA GTACTTCAGA TAGCAGACCA 
AAGAGTAGCA GCCCTATCAG ATTACCTGAA ATGAGTGGAG GACAGACAAA TCGTACAACA 
GAAACAGAAC CACAACCAAC CAAAAAGGCT TCTGGAATGT TGTCCTTCTT CAGAGGGACA 
GCAGGGAAAA GCCCCGATCT CTCTTCCCAG AAGAGAGAGA CCTTACGTGG AGCAGATAGT 
GCTTACTACC AGGTTGGGCA GACGGGCAAG GAGGGGACCG AGAATCAAGG CGTTGAGCCT 
CAAGATGAAG TAGATGGAGG AGATACACAA AAGAAGCAAC TCATAAATCC TCATGTGGAA 
CTTCAATTTT CAGATGCTAA TGCCAAGTTT TACTGTCGGC TCTACTATGC GGGAGAGTTT 
CATAAGATGC GTGAAGTGAT TCTGGACAGC AGTGAAGAAG ATTTCATTCG TTCCCTCTCC 
CACTCATCAC CCTGGCAGGC CCGGGGAGGC AAATCAGGAG CTGCCTTCTA TGCAACTGAG 
GATGATAGAT TTATTTTGAA GCAAATGCCT CGTCTGGAAG TCCAGTCCTT CCTCGACTTT 
GCACCACATT ACTTCAATTA TATTACAAAT GCTGTTCAAC AAAAGAGGCC CACGGCGTTG 
GCCAAAATTC TTGGAGTTTA CAGAATTGGT TATAAGAACT CTCAGAACAA CACTGAGAAG 
AAGTTAGATC TCCTTGTCAT GGAAAATCTT TTCTACGGGA GAAAGATGGC ACAGGTTTTT 
GATTTGAAGG GCTCTCTTAG GAATCGGAAT GTAAAAACTG ACACTGGAAA AGAGAGTTGT 
GATGTGGTCC TGCTAGATGA AAATCTCCTA AAGATGGTTC GAGACAACCC TCTATATATT 
CGTTCTCATT CCAAAGCTGT GCTGAGAACC TCGATCCATA GTGACTCCCA TTTCCTTTCT 
AGCCACCTCA TTATAGATTA TTCTTTGCTG GTTGGGCGAG ATGATACTAG CAATGAGCTA 
GTAGTTGGAA TTATAGATTA TATTCGAACA TTTACATGGG ACAAAAAGCT TGAGATGGTT 
GTGAAATCAA CAGGAATTTT AGGTGGACAA GGTAAAATGC CAACAGTGGT GTCTCCGGAG 
TTGTACAGGA CTAGGTTTTG TGAGGCAATG GACAAGTATT TCCTAATGGT ACCAGACCAC 
TGGACAGGCT TGGGTCTGAA TTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011509087.1
CDS	131..6277
Translation	MATDDKTSPTLDSANDLPRSPTSPSHLTHFKPLTPDQDEPPFKSAYSSFVNLFRFNKERAEGGQGEQQPLSGSWTSPQLPSRTQSVRSPTPYKKQLNEELQRRSSALADNSLQHPQENTDTRRKAEPTFGGHDPRTAVQLRSLSTVLKRLKEIMEGKSQDSDLKQYWMPDSQCKECYDCSEKFTTFRRRHHCRLCGQIFCSRCCNQEIPGKFMGYTGDLRACTYCRKIALSYAHSTDSNSIGEDLNALSDSACSVSVLDPSEPRTPVGSRKASRNIFLEDDLAWQSLIHPDSSNTPLSTRLVSVQEDAGKSPARNRSASITNLSLDRSGSPMVPSYETSVSPQANRTYVRTETTEDERKILLDSVQLKDLWKKICHHSSGMEFQDHRYWLRTHPNCIVGKELVNWLIRNGHIATRAQAIAIGQAMVDGRWLDCVSHHDQLFRDEYALYRPLQSTEFSETPSPDSDSVNSVEGHSEPSWFKDIKFDDSDTEQIAEEEYLISDTGGQQLSISDAFIKESLFNRRVEEKSKELPFTPLGWHHNNLELLREENGEKQAMERLLSANHNHMMALLQQLLHSDSLSSSWRDIIVSLVCQVVQTVRPDVKNQDDDMDIRQFVHIKKIPGGKKFDSVVVNGFVCTKNIAHKKMSSCIKNPKILLLKCSIEYLYREETKFTCIDPIVLQEREFLKNYVQRIVDVRPTLVLVEKTVSRIAQDMLLEHGITLVINVKSQVLERISRMTQGDLVMSMDQLLTKPHLGTCHKFYMQIFQLPNEQTKTLMFFEGCPQHLGCTIKLRGGSDYELARVKEILIFMICVAYHSQLEISFLMDEFAMPPTLMQNPSFHSLIEGRGHEGAVQEQYGGGSIPWDPDIPPESLPCDDSSLLELRIVFEKGEQENKNLPQAVASVKHQEHSTTACPAGLPCAFFAPVPESLLPLPVDDQQDALGSEQPETLQQTVVLQDPKSQIRAFRDPLQDDTGLYVTEEVTSSEDKRKTYSLAFKQELKDVILCISPVITFREPFLLTEKGMRCSTRDYFAEQVYWSPLLNKEFKEMENRRKKQLLRDLSGLQGMNGSIQAKSIQVLPSHELVSTRIAEHLGDSQSLGRMLADYRARGGRIQPKNSDPFAHSKDASSTSSGQSGSKNEGDEERGLILSDAVWSTKVDCLNPINHQRLCVLFSSSSAQSSNAPSACVSPWIVTMEFYGKNDLTLGIFLERYCFRPSYQCPSMFCDTPMVHHIRRFVHGQGCVQIILKELDSPVPGYQHTILTYSWCRICKQVTPVVALSNESWSMSFAKYLELRFYGHQYTRRANAEPCGHSIHHDYHQYFSYNQMVASFSYSPIRLLEVCVPLPKIFIKRQAPLKVSLLQDLKDFFQKVSQVYVAIDERLASLKTDTFSKTREEKMEDIFAQKEMEEGEFKNWIEKMQARLMSSSVDTPQQLQSVFESLIAKKQSLCEVLQAWNNRLQDLFQQEKGRKRPSVPPSPGRLRQGEESKISAMDASPRNISPGLQNGEKEDRFLTTLSSQSSTSSTHLQLPTPPEVMSEQSVGGPPELDTASSSEDVFDGHLLGSTDSQVKEKSTMKAIFANLLPGNSYNPIPFPFDPDKHYLMYEHERVPIAVCEKEPSSIIAFALSCKEYRNALEELSKATQWNSAEEGLPTNSTSDSRPKSSSPIRLPEMSGGQTNRTTETEPQPTKKASGMLSFFRGTAGKSPDLSSQKRETLRGADSAYYQVGQTGKEGTENQGVEPQDEVDGGDTQKKQLINPHVELQFSDANAKFYCRLYYAGEFHKMREVILDSSEEDFIRSLSHSSPWQARGGKSGAAFYATEDDRFILKQMPRLEVQSFLDFAPHYFNYITNAVQQKRPTALAKILGVYRIGYKNSQNNTEKKLDLLVMENLFYGRKMAQVFDLKGSLRNRNVKTDTGKESCDVVLLDENLLKMVRDNPLYIRSHSKAVLRTSIHSDSHFLSSHLIIDYSLLVGRDDTSNELVVGIIDYIRTFTWDKKLEMVVKSTGILGGQGKMPTVVSPELYRTRFCEAMDKYFLMVPDHWTGLGLNC

Target ORF information:

RefSeq Version	XM_011510785.2
Organism	Homo sapiens(human)
Definition	Homo sapiens phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011510785.2

ORF Insert Sequence:

ATGGCCACAG ATGATAAGAC GTCCCCAACA CTGGACTCTG CTAATGATTT GCCTCGATCT 
CCTACTAGTC CTTCTCATCT CACACACTTT AAACCTTTGA CTCCTGATCA AGATGAGCCC 
CCTTTTAAAT CAGCTTATAG TTCTTTTGTA AATCTCTTTC GTTTTAACAA AGAGAGAGCA 
GAAGGAGGCC AGGGAGAACA GCAGCCTTTG AGTGGAAGTT GGACCAGCCC TCAGCTCCCT 
TCGAGGACAC AGTCTGTTAG GTCACCCACA CCTTATAAAA AGCAGCTTAA TGAGGAACTC 
CAGCGGCGCT CTTCAGCATT AGCAGACAAC AGTTTGCAAC ATCCCCAGGA GAACACAGAC 
ACAAGAAGGA AAGCAGAACC TACCTTTGGA GGTCATGACC CTCGTACAGC TGTTCAGCTT 
CGAAGCCTCA GCACAGTATT AAAACGCCTC AAGGAAATCA TGGAGGGGAA AAGCCAGGAT 
AGTGACCTGA AACAATACTG GATGCCAGAT AGCCAATGTA AAGAGTGCTA TGACTGTAGT 
GAGAAATTTA CAACCTTTAG GCGCAGACAC CATTGCCGAC TATGTGGGCA GATTTTCTGC 
AGTCGTTGCT GTAATCAAGA AATCCCTGGA AAATTTATGG GCTATACAGG AGACCTCCGA 
GCTTGCACAT ATTGTAGAAA AATAGCCTTA AGTTATGCTC ATTCCACAGA CAGTAATTCT 
ATTGGGGAAG ACTTGAATGC TCTTTCAGAT TCTGCTTGCT CTGTGTCTGT GCTTGATCCA 
AGTGAACCCC GAACACCTGT TGGGAGTAGG AAAGCCAGCC GTAACATATT TTTAGAGGAT 
GATTTGGCCT GGCAAAGTTT GATTCATCCA GATTCCTCAA ATACTCCTCT TTCAACAAGA 
CTTGTATCTG TGCAAGAGGA TGCTGGGAAA TCTCCTGCTC GAAATAGATC AGCCAGCATT 
ACTAACCTGT CACTGGATAG ATCTGGTTCT CCTATGGTAC CTTCATATGA GACATCTGTC 
AGTCCCCAGG CTAACCGAAC ATATGTTAGG ACAGAGACCA CTGAGGATGA ACGCAAAATT 
CTTCTGGACA GTGTGCAGTT AAAAGACCTG TGGAAAAAAA TCTGCCATCA CAGCAGTGGA 
ATGGAGTTTC AGGATCACCG CTACTGGTTG AGAACGCATC CCAACTGCAT TGTAGGAAAG 
GAATTAGTCA ACTGGCTAAT CCGAAATGGG CATATTGCCA CAAGGGCACA AGCTATAGCA 
ATTGGACAAG CAATGGTTGA TGGACGTTGG CTGGATTGTG TTAGTCATCA CGACCAGCTT 
TTCAGAGATG AGTATGCGCT GTATAGACCA CTGCAGAGTA CAGAATTTTC TGAGACGCCT 
TCTCCCGACA GTGACTCAGT GAACTCCGTG GAAGGACACT CTGAGCCATC CTGGTTTAAA 
GACATAAAGT TTGATGACAG TGACACAGAA CAGATAGCTG AAGAAGAGTA TTTGATTTCT 
GACACTGGAG GACAACAGCT CTCAATAAGT GACGCTTTCA TCAAAGAATC CTTATTTAAT 
CGCCGAGTAG AGGAAAAATC CAAAGAGCTG CCTTTCACAC CTTTGGGCTG GCATCATAAC 
AACCTGGAGC TCCTGAGGGA GGAGAATGGG GAGAAACAAG CCATGGAGAG GTTGCTTTCA 
GCTAATCATA ACCACATGAT GGCACTACTC CAGCAGTTGC TCCATAGTGA CTCACTGTCA 
TCATCTTGGA GGGACATCAT CGTGTCATTG GTCTGCCAGG TTGTTCAGAC AGTCCGACCT 
GATGTCAAGA ACCAGGATGA TGACATGGAT ATCCGTCAGT TTGTCCACAT CAAAAAAATC 
CCAGGTGGAA AGAAGTTTGA TTCTGTGGTT GTCAATGGCT TTGTTTGTAC CAAGAACATT 
GCACATAAAA AGATGAGTTC TTGTATTAAA AACCCCAAAA TTCTTCTGTT GAAGTGTTCC 
ATTGAGTATC TCTACAGAGA AGAAACTAAG TTTACTTGCA TTGATCCTAT TGTGCTTCAG 
GAAAGGGAAT TCTTGAAGAA TTATGTCCAG CGAATAGTTG ATGTTCGACC CACCTTGGTT 
CTTGTTGAGA AAACAGTGTC TCGGATTGCC CAGGACATGT TATTGGAACA TGGCATTACT 
TTGGTCATTA ATGTAAAGTC ACAAGTTTTG GAACGAATCA GTCGAATGAC CCAAGGTGAT 
TTAGTGATGT CAATGGACCA GCTGCTTACG AAACCACACC TGGGCACTTG TCACAAATTT 
TATATGCAGA TATTTCAGTT GCCTAATGAA CAAACCAAGA CACTGATGTT TTTTGAAGGT 
TGTCCACAGC ACCTAGGCTG TACAATCAAG CTAAGAGGAG GCTCTGATTA TGAGCTGGCT 
CGAGTTAAGG AGATCCTAAT ATTTATGATC TGTGTTGCTT ATCATTCTCA ACTAGAAATA 
TCCTTTCTCA TGGATGAATT TGCTATGCCT CCCACATTAA TGCAAAACCC TTCATTCCAT 
TCCCTGATTG AGGGACGAGG GCATGAGGGG GCTGTCCAAG AGCAGTACGG TGGAGGTTCC 
ATCCCCTGGG ATCCTGACAT CCCTCCTGAG TCTCTGCCCT GTGATGATAG CAGTTTGCTG 
GAATTGAGGA TTGTGTTTGA GAAGGGTGAG CAGGAAAATA AAAATCTTCC GCAGGCTGTT 
GCCTCTGTGA AGCATCAAGA ACATAGCACA ACAGCTTGCC CGGCGGGTCT CCCTTGTGCT 
TTCTTTGCAC CTGTACCGGA ATCATTGTTG CCACTCCCTG TGGATGACCA ACAAGATGCT 
TTAGGCAGCG AGCAGCCAGA GACTTTGCAG CAAACAGTTG TGCTGCAGGA TCCCAAAAGC 
CAGATAAGAG CCTTTAGAGA CCCTCTACAG GATGACACTG GATTATATGT TACTGAGGAA 
GTCACCTCCT CTGAAGATAA ACGAAAGACT TATTCTTTGG CCTTTAAGCA GGAATTAAAA 
GATGTGATCC TCTGTATCTC CCCAGTAATC ACATTCCGAG AACCCTTTCT TTTAACTGAA 
AAGGGGATGA GATGCTCTAC CCGAGATTAT TTTGCAGAGC AGGTTTACTG GTCTCCTCTC 
CTCAATAAAG AATTCAAAGA AATGGAGAAC AGGAGGAAGA AACAGCTGCT CAGGGATCTC 
TCTGGACTTC AGGGCATGAA TGGAAGTATT CAGGCCAAGT CTATTCAAGT CTTACCCTCA 
CATGAGCTAG TGAGCACTAG AATTGCTGAG CATCTGGGCG ATAGCCAGAG CTTGGGTAGA 
ATGCTGGCCG ATTATCGAGC CAGAGGAGGA AGAATTCAGC CCAAAAATTC AGACCCTTTT 
GCTCATTCAA AGGATGCATC AAGTACTTCA AGTGGCCAAT CAGGAAGCAA AAATGAGGGT 
GATGAAGAGA GAGGGCTTAT TCTGAGTGAT GCTGTGTGGT CAACAAAGGT GGACTGTCTG 
AATCCCATTA ATCACCAGAG ACTTTGTGTG CTCTTCAGCA GCTCTTCTGC CCAGTCCAGC 
AATGCTCCTA GTGCCTGTGT CAGTCCTTGG ATTGTAACAA TGGAATTTTA TGGAAAGAAT 
GATCTTACAT TAGGAATATT TTTAGAGAGA TACTGTTTCA GGCCTTCTTA TCAGTGTCCA 
AGCATGTTCT GTGATACCCC CATGGTACAT CATATTCGGC GCTTTGTTCA TGGCCAAGGC 
TGTGTGCAGA TAATCCTGAA GGAGTTGGAT TCTCCAGTAC CTGGATATCA GCATACAATT 
CTTACATATT CCTGGTGTAG AATCTGCAAA CAGGTAACAC CAGTTGTTGC TCTTTCCAAT 
GAGTCCTGGT CTATGTCATT TGCAAAATAC CTTGAACTTA GGTTTTATGG GCACCAGTAT 
ACTCGCAGAG CCAACGCTGA GCCCTGTGGT CACTCCATCC ATCATGATTA TCACCAGTAT 
TTCTCCTATA ACCAGATGGT GGCGTCTTTC AGTTATTCTC CCATTCGGCT TCTTGAAGTA 
TGTGTTCCAC TCCCCAAAAT ATTCATTAAG CGTCAGGCCC CATTAAAAGT GTCCCTTCTT 
CAGGATCTGA AGGACTTCTT TCAAAAAGTT TCACAGGTAT ATGTTGCCAT TGATGAAAGA 
CTTGCATCTT TGAAAACTGA TACATTTAGT AAAACAAGAG AGGAAAAAAT GGAAGATATT 
TTTGCACAGA AAGAGATGGA AGAAGGTGAG TTCAAGAACT GGATTGAGAA GATGCAAGCA 
AGGCTCATGT CTTCCTCTGT AGATACCCCT CAGCAACTGC AGTCGGTCTT TGAGTCACTC 
ATTGCCAAGA AACAAAGTCT CTGTGAAGTG CTGCAAGCTT GGAATAACAG GTTGCAGGAC 
CTTTTCCAAC AGGAAAAGGG TAGAAAGAGA CCTTCAGTTC CTCCAAGTCC TGGAAGACTG 
AGACAAGGGG AAGAAAGCAA GATAAGTGCG ATGGATGCAT CTCCACGGAA TATTTCTCCA 
GGACTTCAGA ATGGAGAAAA AGAGGATCGC TTCTTAACAA CTTTGTCCAG CCAGAGCTCC 
ACCAGTTCTA CTCATCTCCA ATTGCCTACG CCACCTGAAG TCATGTCTGA ACAGTCAGTG 
GGAGGGCCCC CTGAGCTAGA TACAGCCAGC AGTTCCGAAG ATGTGTTTGA TGGGCATTTG 
CTGGGATCCA CAGACAGCCA AGTGAAGGAA AAGTCAACCA TGAAAGCCAT CTTTGCAAAT 
TTGCTTCCAG GAAATAGCTA TAATCCTATT CCATTTCCTT TTGATCCAGA TAAACACTAC 
TTAATGTATG AACATGAACG AGTGCCCATT GCAGTCTGCG AGAAGGAACC CAGCTCCATC 
ATTGCTTTTG CTCTCAGTTG TAAAGAATAC CGAAATGCCT TAGAGGAATT GTCTAAAGCG 
ACTCAGTGGA ACAGTGCCGA AGAAGGGCTT CCAACAAATA GTACTTCAGA TAGCAGACCA 
AAGAGTAGCA GCCCTATCAG ATTACCTGAA ATGAGTGGAG GACAGACAAA TCGTACAACA 
GAAACAGAAC CACAACCAAC CAAAAAGGCT TCTGGAATGT TGTCCTTCTT CAGAGGGACA 
GCAGGGAAAA GCCCCGATCT CTCTTCCCAG AAGAGAGAGA CCTTACGTGG AGCAGATAGT 
GCTTACTACC AGGTTGGGCA GACGGGCAAG GAGGGGACCG AGAATCAAGG CGTTGAGCCT 
CAAGATGAAG TAGATGGAGG AGATACACAA AAGAAGCAAC TCATAAATCC TCATGTGGAA 
CTTCAATTTT CAGATGCTAA TGCCAAGTTT TACTGTCGGC TCTACTATGC GGGAGAGTTT 
CATAAGATGC GTGAAGTGAT TCTGGACAGC AGTGAAGAAG ATTTCATTCG TTCCCTCTCC 
CACTCATCAC CCTGGCAGGC CCGGGGAGGC AAATCAGGAG CTGCCTTCTA TGCAACTGAG 
GATGATAGAT TTATTTTGAA GCAAATGCCT CGTCTGGAAG TCCAGTCCTT CCTCGACTTT 
GCACCACATT ACTTCAATTA TATTACAAAT GCTGTTCAAC AAAAGAGGCC CACGGCGTTG 
GCCAAAATTC TTGGAGTTTA CAGAATTGGT TATAAGAACT CTCAGAACAA CACTGAGAAG 
AAGTTAGATC TCCTTGTCAT GGAAAATCTT TTCTACGGGA GAAAGATGGC ACAGGTTTTT 
GATTTGAAGG GCTCTCTTAG GAATCGGAAT GTAAAAACTG ACACTGGAAA AGAGAGTTGT 
GATGTGGTCC TGCTAGATGA AAATCTCCTA AAGATGGTTC GAGACAACCC TCTATATATT 
CGTTCTCATT CCAAAGCTGT GCTGAGAACC TCGATCCATA GTGACTCCCA TTTCCTTTCT 
AGCCACCTCA TTATAGATTA TTCTTTGCTG GTTGGGCGAG ATGATACTAG CAATGAGCTA 
GTAGTTGGAA TTATAGATTA TATTCGAACA TTTACATGGG ACAAAAAGCT TGAGATGGTT 
GTGAAATCAA CAGGAATTTT AGGTGGACAA GGTAAAATGC CAACAGTGGT GTCTCCGGAG 
TTGTACAGGA CTAGGTTTTG TGAGGCAATG GACAAGTATT TCCTAATGGT ACCAGACCAC 
TGGACAGGCT TGGGTCTGAA TTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PIKFYVE ( XM_011510786.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011509088.1 Homo sapiens phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X11, mRNA.

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CloneID	OHu77668
Clone ID Related Accession (Same CDS sequence)	XM_011510786.2 , XM_011510786.3
Accession Version	XM_011510786.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6042bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	1-phosphatidylinositol 3-phosphate 5-kinase isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011510786.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG ATGATAAGAC GTCCCCAACA CTGGACTCTG CTAATGATTT GCCTCGATCT 
CCTACTAGTC CTTCTCATCT CACACACTTT AAACCTTTGA CTCCTGATCA AGATGAGCCC 
CCTTTTAAAT CAGCTTATAG TTCTTTTGTA AATCTCTTTC GTTTTAACAA AGAGAGAGCA 
GAAGGAGGCC AGGGAGAACA GCAGCCTTTG AGTGGAAGTT GGACCAGCCC TCAGCTCCCT 
TCGAGGACAC AGTCTGTTAG GTCACCCACA CCTTATAAAA AGCAGCTTAA TGAGGAACTC 
CAGCGGCGCT CTTCAGCATT AGCAGACAAC AGTTTGCAAC ATCCCCAGGA GAACACAGGA 
GACCTCCGAG CTTGCACATA TTGTAGAAAA ATAGCCTTAA GTTATGCTCA TTCCACAGAC 
AGTAATTCTA TTGGGGAAGA CTTGAATGCT CTTTCAGATT CTGCTTGCTC TGTGTCTGTG 
CTTGATCCAA GTGAACCCCG AACACCTGTT GGGAGTAGGA AAGCCAGCCG TAACATATTT 
TTAGAGGATG ATTTGGCCTG GCAAAGTTTG ATTCATCCAG ATTCCTCAAA TACTCCTCTT 
TCAACAAGAC TTGTATCTGT GCAAGAGGAT GCTGGGAAAT CTCCTGCTCG AAATAGATCA 
GCCAGCATTA CTAACCTGTC ACTGGATAGA TCTGGTTCTC CTATGGTACC TTCATATGAG 
ACATCTGTCA GTCCCCAGGC TAACCGAACA TATGTTAGGA CAGAGACCAC TGAGGATGAA 
CGCAAAATTC TTCTGGACAG TGTGCAGTTA AAAGACCTGT GGAAAAAAAT CTGCCATCAC 
AGCAGTGGAA TGGAGTTTCA GGATCACCGC TACTGGTTGA GAACGCATCC CAACTGCATT 
GTAGGAAAGG AATTAGTCAA CTGGCTAATC CGAAATGGGC ATATTGCCAC AAGGGCACAA 
GCTATAGCAA TTGGACAAGC AATGGTTGAT GGACGTTGGC TGGATTGTGT TAGTCATCAC 
GACCAGCTTT TCAGAGATGA GTATGCGCTG TATAGACCAC TGCAGAGTAC AGAATTTTCT 
GAGACGCCTT CTCCCGACAG TGACTCAGTG AACTCCGTGG AAGGACACTC TGAGCCATCC 
TGGTTTAAAG ACATAAAGTT TGATGACAGT GACACAGAAC AGATAGCTGA AGAAGGTGAC 
GATAATTTGG CTAATTCTGC CAGTCCTAGC AAGCGCACAT CAGTCAGCAG TTTCCAGTCC 
ACAGTGGACA GTGACTCAGC CGCTTCTATC AGCCTGAACG TGGAGCTGGA CAACGTGAAC 
TTCCATATCA AGAAGCCCTC CAAGTACCCA CATGTGCCCC CTCACCCTGC TGACCAAAAA 
GAGTATTTGA TTTCTGACAC TGGAGGACAA CAGCTCTCAA TAAGTGACGC TTTCATCAAA 
GAATCCTTAT TTAATCGCCG AGTAGAGGAA AAATCCAAAG AGCTGCCTTT CACACCTTTG 
GGCTGGCATC ATAACAACCT GGAGCTCCTG AGGGAGGAGA ATGGGGAGAA ACAAGCCATG 
GAGAGGTTGC TTTCAGCTAA TCATAACCAC ATGATGGCAC TACTCCAGCA GTTGCTCCAT 
AGTGACTCAC TGTCATCATC TTGGAGGGAC ATCATCGTGT CATTGGTCTG CCAGGTTGTT 
CAGACAGTCC GACCTGATGT CAAGAACCAG GATGATGACA TGGATATCCG TCAGTTTGTC 
CACATCAAAA AAATCCCAGG TGGAAAGAAG TTTGATTCTG TGGTTGTCAA TGGCTTTGTT 
TGTACCAAGA ACATTGCACA TAAAAAGATG AGTTCTTGTA TTAAAAACCC CAAAATTCTT 
CTGTTGAAGT GTTCCATTGA GTATCTCTAC AGAGAAGAAA CTAAGTTTAC TTGCATTGAT 
CCTATTGTGC TTCAGGAAAG GGAATTCTTG AAGAATTATG TCCAGCGAAT AGTTGATGTT 
CGACCCACCT TGGTTCTTGT TGAGAAAACA GTGTCTCGGA TTGCCCAGGA CATGTTATTG 
GAACATGGCA TTACTTTGGT CATTAATGTA AAGTCACAAG TTTTGGAACG AATCAGTCGA 
ATGACCCAAG GTGATTTAGT GATGTCAATG GACCAGCTGC TTACGAAACC ACACCTGGGC 
ACTTGTCACA AATTTTATAT GCAGATATTT CAGTTGCCTA ATGAACAAAC CAAGACACTG 
ATGTTTTTTG AAGGTTGTCC ACAGCACCTA GGCTGTACAA TCAAGCTAAG AGGAGGCTCT 
GATTATGAGC TGGCTCGAGT TAAGGAGATC CTAATATTTA TGATCTGTGT TGCTTATCAT 
TCTCAACTAG AAATATCCTT TCTCATGGAT GAATTTGCTA TGCCTCCCAC ATTAATGCAA 
AACCCTTCAT TCCATTCCCT GATTGAGGGA CGAGGGCATG AGGGGGCTGT CCAAGAGCAG 
TACGGTGGAG GTTCCATCCC CTGGGATCCT GACATCCCTC CTGAGTCTCT GCCCTGTGAT 
GATAGCAGTT TGCTGGAATT GAGGATTGTG TTTGAGAAGG GTGAGCAGGA AAATAAAAAT 
CTTCCGCAGG CTGTTGCCTC TGTGAAGCAT CAAGAACATA GCACAACAGC TTGCCCGGCG 
GGTCTCCCTT GTGCTTTCTT TGCACCTGTA CCGGAATCAT TGTTGCCACT CCCTGTGGAT 
GACCAACAAG ATGCTTTAGG CAGCGAGCAG CCAGAGACTT TGCAGCAAAC AGTTGTGCTG 
CAGGATCCCA AAAGCCAGAT AAGAGCCTTT AGAGACCCTC TACAGGATGA CACTGGATTA 
TATGTTACTG AGGAAGTCAC CTCCTCTGAA GATAAACGAA AGACTTATTC TTTGGCCTTT 
AAGCAGGAAT TAAAAGATGT GATCCTCTGT ATCTCCCCAG TAATCACATT CCGAGAACCC 
TTTCTTTTAA CTGAAAAGGG GATGAGATGC TCTACCCGAG ATTATTTTGC AGAGCAGGTT 
TACTGGTCTC CTCTCCTCAA TAAAGAATTC AAAGAAATGG AGAACAGGAG GAAGAAACAG 
CTGCTCAGGG ATCTCTCTGG ACTTCAGGGC ATGAATGGAA GTATTCAGGC CAAGTCTATT 
CAAGTCTTAC CCTCACATGA GCTAGTGAGC ACTAGAATTG CTGAGCATCT GGGCGATAGC 
CAGAGCTTGG GTAGAATGCT GGCCGATTAT CGAGCCAGAG GAGGAAGAAT TCAGCCCAAA 
AATTCAGACC CTTTTGCTCA TTCAAAGGAT GCATCAAGTA CTTCAAGTGG CCAATCAGGA 
AGCAAAAATG AGGGTGATGA AGAGAGAGGG CTTATTCTGA GTGATGCTGT GTGGTCAACA 
AAGGTGGACT GTCTGAATCC CATTAATCAC CAGAGACTTT GTGTGCTCTT CAGCAGCTCT 
TCTGCCCAGT CCAGCAATGC TCCTAGTGCC TGTGTCAGTC CTTGGATTGT AACAATGGAA 
TTTTATGGAA AGAATGATCT TACATTAGGA ATATTTTTAG AGAGATACTG TTTCAGGCCT 
TCTTATCAGT GTCCAAGCAT GTTCTGTGAT ACCCCCATGG TACATCATAT TCGGCGCTTT 
GTTCATGGCC AAGGCTGTGT GCAGATAATC CTGAAGGAGT TGGATTCTCC AGTACCTGGA 
TATCAGCATA CAATTCTTAC ATATTCCTGG TGTAGAATCT GCAAACAGGT AACACCAGTT 
GTTGCTCTTT CCAATGAGTC CTGGTCTATG TCATTTGCAA AATACCTTGA ACTTAGGTTT 
TATGGGCACC AGTATACTCG CAGAGCCAAC GCTGAGCCCT GTGGTCACTC CATCCATCAT 
GATTATCACC AGTATTTCTC CTATAACCAG ATGGTGGCGT CTTTCAGTTA TTCTCCCATT 
CGGCTTCTTG AAGTATGTGT TCCACTCCCC AAAATATTCA TTAAGCGTCA GGCCCCATTA 
AAAGTGTCCC TTCTTCAGGA TCTGAAGGAC TTCTTTCAAA AAGTTTCACA GGTATATGTT 
GCCATTGATG AAAGACTTGC ATCTTTGAAA ACTGATACAT TTAGTAAAAC AAGAGAGGAA 
AAAATGGAAG ATATTTTTGC ACAGAAAGAG ATGGAAGAAG GTGAGTTCAA GAACTGGATT 
GAGAAGATGC AAGCAAGGCT CATGTCTTCC TCTGTAGATA CCCCTCAGCA ACTGCAGTCG 
GTCTTTGAGT CACTCATTGC CAAGAAACAA AGTCTCTGTG AAGTGCTGCA AGCTTGGAAT 
AACAGGTTGC AGGACCTTTT CCAACAGGAA AAGGGTAGAA AGAGACCTTC AGTTCCTCCA 
AGTCCTGGAA GACTGAGACA AGGGGAAGAA AGCAAGATAA GTGCGATGGA TGCATCTCCA 
CGGAATATTT CTCCAGGACT TCAGAATGGA GAAAAAGAGG ATCGCTTCTT AACAACTTTG 
TCCAGCCAGA GCTCCACCAG TTCTACTCAT CTCCAATTGC CTACGCCACC TGAAGTCATG 
TCTGAACAGT CAGTGGGAGG GCCCCCTGAG CTAGATACAG CCAGCAGTTC CGAAGATGTG 
TTTGATGGGC ATTTGCTGGG ATCCACAGAC AGCCAAGTGA AGGAAAAGTC AACCATGAAA 
GCCATCTTTG CAAATTTGCT TCCAGGAAAT AGCTATAATC CTATTCCATT TCCTTTTGAT 
CCAGATAAAC ACTACTTAAT GTATGAACAT GAACGAGTGC CCATTGCAGT CTGCGAGAAG 
GAACCCAGCT CCATCATTGC TTTTGCTCTC AGTTGTAAAG AATACCGAAA TGCCTTAGAG 
GAATTGTCTA AAGCGACTCA GTGGAACAGT GCCGAAGAAG GGCTTCCAAC AAATAGTACT 
TCAGATAGCA GACCAAAGAG TAGCAGCCCT ATCAGATTAC CTGAAATGAG TGGAGGACAG 
ACAAATCGTA CAACAGAAAC AGAACCACAA CCAACCAAAA AGGCTTCTGG AATGTTGTCC 
TTCTTCAGAG GGACAGCAGG GAAAAGCCCC GATCTCTCTT CCCAGAAGAG AGAGACCTTA 
CGTGGAGCAG ATAGTGCTTA CTACCAGGTT GGGCAGACGG GCAAGGAGGG GACCGAGAAT 
CAAGGCGTTG AGCCTCAAGA TGAAGTAGAT GGAGGAGATA CACAAAAGAA GCAACTCATA 
AATCCTCATG TGGAACTTCA ATTTTCAGAT GCTAATGCCA AGTTTTACTG TCGGCTCTAC 
TATGCGGGAG AGTTTCATAA GATGCGTGAA GTGATTCTGG ACAGCAGTGA AGAAGATTTC 
ATTCGTTCCC TCTCCCACTC ATCACCCTGG CAGGCCCGGG GAGGCAAATC AGGAGCTGCC 
TTCTATGCAA CTGAGGATGA TAGATTTATT TTGAAGCAAA TGCCTCGTCT GGAAGTCCAG 
TCCTTCCTCG ACTTTGCACC ACATTACTTC AATTATATTA CAAATGCTGT TCAACAAAAG 
AGGCCCACGG CGTTGGCCAA AATTCTTGGA GTTTACAGAA TTGGTTATAA GAACTCTCAG 
AACAACACTG AGAAGAAGTT AGATCTCCTT GTCATGGAAA ATCTTTTCTA CGGGAGAAAG 
ATGGCACAGG TTTTTGATTT GAAGGGCTCT CTTAGGAATC GGAATGTAAA AACTGACACT 
GGAAAAGAGA GTTGTGATGT GGTCCTGCTA GATGAAAATC TCCTAAAGAT GGTTCGAGAC 
AACCCTCTAT ATATTCGTTC TCATTCCAAA GCTGTGCTGA GAACCTCGAT CCATAGTGAC 
TCCCATTTCC TTTCTAGCCA CCTCATTATA GATTATTCTT TGCTGGTTGG GCGAGATGAT 
ACTAGCAATG AGCTAGTAGT TGGAATTATA GATTATATTC GAACATTTAC ATGGGACAAA 
AAGCTTGAGA TGGTTGTGAA ATCAACAGGA ATTTTAGGTG GACAAGGTAA AATGCCAACA 
GTGGTGTCTC CGGAGTTGTA CAGGACTAGG TTTTGTGAGG CAATGGACAA GTATTTCCTA 
ATGGTACCAG ACCACTGGAC AGGCTTGGGT CTGAATTGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011509088.1
CDS	131..6172
Translation	MATDDKTSPTLDSANDLPRSPTSPSHLTHFKPLTPDQDEPPFKSAYSSFVNLFRFNKERAEGGQGEQQPLSGSWTSPQLPSRTQSVRSPTPYKKQLNEELQRRSSALADNSLQHPQENTGDLRACTYCRKIALSYAHSTDSNSIGEDLNALSDSACSVSVLDPSEPRTPVGSRKASRNIFLEDDLAWQSLIHPDSSNTPLSTRLVSVQEDAGKSPARNRSASITNLSLDRSGSPMVPSYETSVSPQANRTYVRTETTEDERKILLDSVQLKDLWKKICHHSSGMEFQDHRYWLRTHPNCIVGKELVNWLIRNGHIATRAQAIAIGQAMVDGRWLDCVSHHDQLFRDEYALYRPLQSTEFSETPSPDSDSVNSVEGHSEPSWFKDIKFDDSDTEQIAEEGDDNLANSASPSKRTSVSSFQSTVDSDSAASISLNVELDNVNFHIKKPSKYPHVPPHPADQKEYLISDTGGQQLSISDAFIKESLFNRRVEEKSKELPFTPLGWHHNNLELLREENGEKQAMERLLSANHNHMMALLQQLLHSDSLSSSWRDIIVSLVCQVVQTVRPDVKNQDDDMDIRQFVHIKKIPGGKKFDSVVVNGFVCTKNIAHKKMSSCIKNPKILLLKCSIEYLYREETKFTCIDPIVLQEREFLKNYVQRIVDVRPTLVLVEKTVSRIAQDMLLEHGITLVINVKSQVLERISRMTQGDLVMSMDQLLTKPHLGTCHKFYMQIFQLPNEQTKTLMFFEGCPQHLGCTIKLRGGSDYELARVKEILIFMICVAYHSQLEISFLMDEFAMPPTLMQNPSFHSLIEGRGHEGAVQEQYGGGSIPWDPDIPPESLPCDDSSLLELRIVFEKGEQENKNLPQAVASVKHQEHSTTACPAGLPCAFFAPVPESLLPLPVDDQQDALGSEQPETLQQTVVLQDPKSQIRAFRDPLQDDTGLYVTEEVTSSEDKRKTYSLAFKQELKDVILCISPVITFREPFLLTEKGMRCSTRDYFAEQVYWSPLLNKEFKEMENRRKKQLLRDLSGLQGMNGSIQAKSIQVLPSHELVSTRIAEHLGDSQSLGRMLADYRARGGRIQPKNSDPFAHSKDASSTSSGQSGSKNEGDEERGLILSDAVWSTKVDCLNPINHQRLCVLFSSSSAQSSNAPSACVSPWIVTMEFYGKNDLTLGIFLERYCFRPSYQCPSMFCDTPMVHHIRRFVHGQGCVQIILKELDSPVPGYQHTILTYSWCRICKQVTPVVALSNESWSMSFAKYLELRFYGHQYTRRANAEPCGHSIHHDYHQYFSYNQMVASFSYSPIRLLEVCVPLPKIFIKRQAPLKVSLLQDLKDFFQKVSQVYVAIDERLASLKTDTFSKTREEKMEDIFAQKEMEEGEFKNWIEKMQARLMSSSVDTPQQLQSVFESLIAKKQSLCEVLQAWNNRLQDLFQQEKGRKRPSVPPSPGRLRQGEESKISAMDASPRNISPGLQNGEKEDRFLTTLSSQSSTSSTHLQLPTPPEVMSEQSVGGPPELDTASSSEDVFDGHLLGSTDSQVKEKSTMKAIFANLLPGNSYNPIPFPFDPDKHYLMYEHERVPIAVCEKEPSSIIAFALSCKEYRNALEELSKATQWNSAEEGLPTNSTSDSRPKSSSPIRLPEMSGGQTNRTTETEPQPTKKASGMLSFFRGTAGKSPDLSSQKRETLRGADSAYYQVGQTGKEGTENQGVEPQDEVDGGDTQKKQLINPHVELQFSDANAKFYCRLYYAGEFHKMREVILDSSEEDFIRSLSHSSPWQARGGKSGAAFYATEDDRFILKQMPRLEVQSFLDFAPHYFNYITNAVQQKRPTALAKILGVYRIGYKNSQNNTEKKLDLLVMENLFYGRKMAQVFDLKGSLRNRNVKTDTGKESCDVVLLDENLLKMVRDNPLYIRSHSKAVLRTSIHSDSHFLSSHLIIDYSLLVGRDDTSNELVVGIIDYIRTFTWDKKLEMVVKSTGILGGQGKMPTVVSPELYRTRFCEAMDKYFLMVPDHWTGLGLNC

Target ORF information:

RefSeq Version	XM_011510786.2
Organism	Homo sapiens(human)
Definition	Homo sapiens phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011510786.2

ORF Insert Sequence:

ATGGCCACAG ATGATAAGAC GTCCCCAACA CTGGACTCTG CTAATGATTT GCCTCGATCT 
CCTACTAGTC CTTCTCATCT CACACACTTT AAACCTTTGA CTCCTGATCA AGATGAGCCC 
CCTTTTAAAT CAGCTTATAG TTCTTTTGTA AATCTCTTTC GTTTTAACAA AGAGAGAGCA 
GAAGGAGGCC AGGGAGAACA GCAGCCTTTG AGTGGAAGTT GGACCAGCCC TCAGCTCCCT 
TCGAGGACAC AGTCTGTTAG GTCACCCACA CCTTATAAAA AGCAGCTTAA TGAGGAACTC 
CAGCGGCGCT CTTCAGCATT AGCAGACAAC AGTTTGCAAC ATCCCCAGGA GAACACAGGA 
GACCTCCGAG CTTGCACATA TTGTAGAAAA ATAGCCTTAA GTTATGCTCA TTCCACAGAC 
AGTAATTCTA TTGGGGAAGA CTTGAATGCT CTTTCAGATT CTGCTTGCTC TGTGTCTGTG 
CTTGATCCAA GTGAACCCCG AACACCTGTT GGGAGTAGGA AAGCCAGCCG TAACATATTT 
TTAGAGGATG ATTTGGCCTG GCAAAGTTTG ATTCATCCAG ATTCCTCAAA TACTCCTCTT 
TCAACAAGAC TTGTATCTGT GCAAGAGGAT GCTGGGAAAT CTCCTGCTCG AAATAGATCA 
GCCAGCATTA CTAACCTGTC ACTGGATAGA TCTGGTTCTC CTATGGTACC TTCATATGAG 
ACATCTGTCA GTCCCCAGGC TAACCGAACA TATGTTAGGA CAGAGACCAC TGAGGATGAA 
CGCAAAATTC TTCTGGACAG TGTGCAGTTA AAAGACCTGT GGAAAAAAAT CTGCCATCAC 
AGCAGTGGAA TGGAGTTTCA GGATCACCGC TACTGGTTGA GAACGCATCC CAACTGCATT 
GTAGGAAAGG AATTAGTCAA CTGGCTAATC CGAAATGGGC ATATTGCCAC AAGGGCACAA 
GCTATAGCAA TTGGACAAGC AATGGTTGAT GGACGTTGGC TGGATTGTGT TAGTCATCAC 
GACCAGCTTT TCAGAGATGA GTATGCGCTG TATAGACCAC TGCAGAGTAC AGAATTTTCT 
GAGACGCCTT CTCCCGACAG TGACTCAGTG AACTCCGTGG AAGGACACTC TGAGCCATCC 
TGGTTTAAAG ACATAAAGTT TGATGACAGT GACACAGAAC AGATAGCTGA AGAAGGTGAC 
GATAATTTGG CTAATTCTGC CAGTCCTAGC AAGCGCACAT CAGTCAGCAG TTTCCAGTCC 
ACAGTGGACA GTGACTCAGC CGCTTCTATC AGCCTGAACG TGGAGCTGGA CAACGTGAAC 
TTCCATATCA AGAAGCCCTC CAAGTACCCA CATGTGCCCC CTCACCCTGC TGACCAAAAA 
GAGTATTTGA TTTCTGACAC TGGAGGACAA CAGCTCTCAA TAAGTGACGC TTTCATCAAA 
GAATCCTTAT TTAATCGCCG AGTAGAGGAA AAATCCAAAG AGCTGCCTTT CACACCTTTG 
GGCTGGCATC ATAACAACCT GGAGCTCCTG AGGGAGGAGA ATGGGGAGAA ACAAGCCATG 
GAGAGGTTGC TTTCAGCTAA TCATAACCAC ATGATGGCAC TACTCCAGCA GTTGCTCCAT 
AGTGACTCAC TGTCATCATC TTGGAGGGAC ATCATCGTGT CATTGGTCTG CCAGGTTGTT 
CAGACAGTCC GACCTGATGT CAAGAACCAG GATGATGACA TGGATATCCG TCAGTTTGTC 
CACATCAAAA AAATCCCAGG TGGAAAGAAG TTTGATTCTG TGGTTGTCAA TGGCTTTGTT 
TGTACCAAGA ACATTGCACA TAAAAAGATG AGTTCTTGTA TTAAAAACCC CAAAATTCTT 
CTGTTGAAGT GTTCCATTGA GTATCTCTAC AGAGAAGAAA CTAAGTTTAC TTGCATTGAT 
CCTATTGTGC TTCAGGAAAG GGAATTCTTG AAGAATTATG TCCAGCGAAT AGTTGATGTT 
CGACCCACCT TGGTTCTTGT TGAGAAAACA GTGTCTCGGA TTGCCCAGGA CATGTTATTG 
GAACATGGCA TTACTTTGGT CATTAATGTA AAGTCACAAG TTTTGGAACG AATCAGTCGA 
ATGACCCAAG GTGATTTAGT GATGTCAATG GACCAGCTGC TTACGAAACC ACACCTGGGC 
ACTTGTCACA AATTTTATAT GCAGATATTT CAGTTGCCTA ATGAACAAAC CAAGACACTG 
ATGTTTTTTG AAGGTTGTCC ACAGCACCTA GGCTGTACAA TCAAGCTAAG AGGAGGCTCT 
GATTATGAGC TGGCTCGAGT TAAGGAGATC CTAATATTTA TGATCTGTGT TGCTTATCAT 
TCTCAACTAG AAATATCCTT TCTCATGGAT GAATTTGCTA TGCCTCCCAC ATTAATGCAA 
AACCCTTCAT TCCATTCCCT GATTGAGGGA CGAGGGCATG AGGGGGCTGT CCAAGAGCAG 
TACGGTGGAG GTTCCATCCC CTGGGATCCT GACATCCCTC CTGAGTCTCT GCCCTGTGAT 
GATAGCAGTT TGCTGGAATT GAGGATTGTG TTTGAGAAGG GTGAGCAGGA AAATAAAAAT 
CTTCCGCAGG CTGTTGCCTC TGTGAAGCAT CAAGAACATA GCACAACAGC TTGCCCGGCG 
GGTCTCCCTT GTGCTTTCTT TGCACCTGTA CCGGAATCAT TGTTGCCACT CCCTGTGGAT 
GACCAACAAG ATGCTTTAGG CAGCGAGCAG CCAGAGACTT TGCAGCAAAC AGTTGTGCTG 
CAGGATCCCA AAAGCCAGAT AAGAGCCTTT AGAGACCCTC TACAGGATGA CACTGGATTA 
TATGTTACTG AGGAAGTCAC CTCCTCTGAA GATAAACGAA AGACTTATTC TTTGGCCTTT 
AAGCAGGAAT TAAAAGATGT GATCCTCTGT ATCTCCCCAG TAATCACATT CCGAGAACCC 
TTTCTTTTAA CTGAAAAGGG GATGAGATGC TCTACCCGAG ATTATTTTGC AGAGCAGGTT 
TACTGGTCTC CTCTCCTCAA TAAAGAATTC AAAGAAATGG AGAACAGGAG GAAGAAACAG 
CTGCTCAGGG ATCTCTCTGG ACTTCAGGGC ATGAATGGAA GTATTCAGGC CAAGTCTATT 
CAAGTCTTAC CCTCACATGA GCTAGTGAGC ACTAGAATTG CTGAGCATCT GGGCGATAGC 
CAGAGCTTGG GTAGAATGCT GGCCGATTAT CGAGCCAGAG GAGGAAGAAT TCAGCCCAAA 
AATTCAGACC CTTTTGCTCA TTCAAAGGAT GCATCAAGTA CTTCAAGTGG CCAATCAGGA 
AGCAAAAATG AGGGTGATGA AGAGAGAGGG CTTATTCTGA GTGATGCTGT GTGGTCAACA 
AAGGTGGACT GTCTGAATCC CATTAATCAC CAGAGACTTT GTGTGCTCTT CAGCAGCTCT 
TCTGCCCAGT CCAGCAATGC TCCTAGTGCC TGTGTCAGTC CTTGGATTGT AACAATGGAA 
TTTTATGGAA AGAATGATCT TACATTAGGA ATATTTTTAG AGAGATACTG TTTCAGGCCT 
TCTTATCAGT GTCCAAGCAT GTTCTGTGAT ACCCCCATGG TACATCATAT TCGGCGCTTT 
GTTCATGGCC AAGGCTGTGT GCAGATAATC CTGAAGGAGT TGGATTCTCC AGTACCTGGA 
TATCAGCATA CAATTCTTAC ATATTCCTGG TGTAGAATCT GCAAACAGGT AACACCAGTT 
GTTGCTCTTT CCAATGAGTC CTGGTCTATG TCATTTGCAA AATACCTTGA ACTTAGGTTT 
TATGGGCACC AGTATACTCG CAGAGCCAAC GCTGAGCCCT GTGGTCACTC CATCCATCAT 
GATTATCACC AGTATTTCTC CTATAACCAG ATGGTGGCGT CTTTCAGTTA TTCTCCCATT 
CGGCTTCTTG AAGTATGTGT TCCACTCCCC AAAATATTCA TTAAGCGTCA GGCCCCATTA 
AAAGTGTCCC TTCTTCAGGA TCTGAAGGAC TTCTTTCAAA AAGTTTCACA GGTATATGTT 
GCCATTGATG AAAGACTTGC ATCTTTGAAA ACTGATACAT TTAGTAAAAC AAGAGAGGAA 
AAAATGGAAG ATATTTTTGC ACAGAAAGAG ATGGAAGAAG GTGAGTTCAA GAACTGGATT 
GAGAAGATGC AAGCAAGGCT CATGTCTTCC TCTGTAGATA CCCCTCAGCA ACTGCAGTCG 
GTCTTTGAGT CACTCATTGC CAAGAAACAA AGTCTCTGTG AAGTGCTGCA AGCTTGGAAT 
AACAGGTTGC AGGACCTTTT CCAACAGGAA AAGGGTAGAA AGAGACCTTC AGTTCCTCCA 
AGTCCTGGAA GACTGAGACA AGGGGAAGAA AGCAAGATAA GTGCGATGGA TGCATCTCCA 
CGGAATATTT CTCCAGGACT TCAGAATGGA GAAAAAGAGG ATCGCTTCTT AACAACTTTG 
TCCAGCCAGA GCTCCACCAG TTCTACTCAT CTCCAATTGC CTACGCCACC TGAAGTCATG 
TCTGAACAGT CAGTGGGAGG GCCCCCTGAG CTAGATACAG CCAGCAGTTC CGAAGATGTG 
TTTGATGGGC ATTTGCTGGG ATCCACAGAC AGCCAAGTGA AGGAAAAGTC AACCATGAAA 
GCCATCTTTG CAAATTTGCT TCCAGGAAAT AGCTATAATC CTATTCCATT TCCTTTTGAT 
CCAGATAAAC ACTACTTAAT GTATGAACAT GAACGAGTGC CCATTGCAGT CTGCGAGAAG 
GAACCCAGCT CCATCATTGC TTTTGCTCTC AGTTGTAAAG AATACCGAAA TGCCTTAGAG 
GAATTGTCTA AAGCGACTCA GTGGAACAGT GCCGAAGAAG GGCTTCCAAC AAATAGTACT 
TCAGATAGCA GACCAAAGAG TAGCAGCCCT ATCAGATTAC CTGAAATGAG TGGAGGACAG 
ACAAATCGTA CAACAGAAAC AGAACCACAA CCAACCAAAA AGGCTTCTGG AATGTTGTCC 
TTCTTCAGAG GGACAGCAGG GAAAAGCCCC GATCTCTCTT CCCAGAAGAG AGAGACCTTA 
CGTGGAGCAG ATAGTGCTTA CTACCAGGTT GGGCAGACGG GCAAGGAGGG GACCGAGAAT 
CAAGGCGTTG AGCCTCAAGA TGAAGTAGAT GGAGGAGATA CACAAAAGAA GCAACTCATA 
AATCCTCATG TGGAACTTCA ATTTTCAGAT GCTAATGCCA AGTTTTACTG TCGGCTCTAC 
TATGCGGGAG AGTTTCATAA GATGCGTGAA GTGATTCTGG ACAGCAGTGA AGAAGATTTC 
ATTCGTTCCC TCTCCCACTC ATCACCCTGG CAGGCCCGGG GAGGCAAATC AGGAGCTGCC 
TTCTATGCAA CTGAGGATGA TAGATTTATT TTGAAGCAAA TGCCTCGTCT GGAAGTCCAG 
TCCTTCCTCG ACTTTGCACC ACATTACTTC AATTATATTA CAAATGCTGT TCAACAAAAG 
AGGCCCACGG CGTTGGCCAA AATTCTTGGA GTTTACAGAA TTGGTTATAA GAACTCTCAG 
AACAACACTG AGAAGAAGTT AGATCTCCTT GTCATGGAAA ATCTTTTCTA CGGGAGAAAG 
ATGGCACAGG TTTTTGATTT GAAGGGCTCT CTTAGGAATC GGAATGTAAA AACTGACACT 
GGAAAAGAGA GTTGTGATGT GGTCCTGCTA GATGAAAATC TCCTAAAGAT GGTTCGAGAC 
AACCCTCTAT ATATTCGTTC TCATTCCAAA GCTGTGCTGA GAACCTCGAT CCATAGTGAC 
TCCCATTTCC TTTCTAGCCA CCTCATTATA GATTATTCTT TGCTGGTTGG GCGAGATGAT 
ACTAGCAATG AGCTAGTAGT TGGAATTATA GATTATATTC GAACATTTAC ATGGGACAAA 
AAGCTTGAGA TGGTTGTGAA ATCAACAGGA ATTTTAGGTG GACAAGGTAA AATGCCAACA 
GTGGTGTCTC CGGAGTTGTA CAGGACTAGG TTTTGTGAGG CAATGGACAA GTATTTCCTA 
ATGGTACCAG ACCACTGGAC AGGCTTGGGT CTGAATTGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PIKFYVE ( XM_011510786.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011509088.1 Homo sapiens phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X11, mRNA.

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CloneID	OHu77668
Clone ID Related Accession (Same CDS sequence)	XM_011510786.2 , XM_011510786.3
Accession Version	XM_011510786.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6042bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	1-phosphatidylinositol 3-phosphate 5-kinase isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011510786.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG ATGATAAGAC GTCCCCAACA CTGGACTCTG CTAATGATTT GCCTCGATCT 
CCTACTAGTC CTTCTCATCT CACACACTTT AAACCTTTGA CTCCTGATCA AGATGAGCCC 
CCTTTTAAAT CAGCTTATAG TTCTTTTGTA AATCTCTTTC GTTTTAACAA AGAGAGAGCA 
GAAGGAGGCC AGGGAGAACA GCAGCCTTTG AGTGGAAGTT GGACCAGCCC TCAGCTCCCT 
TCGAGGACAC AGTCTGTTAG GTCACCCACA CCTTATAAAA AGCAGCTTAA TGAGGAACTC 
CAGCGGCGCT CTTCAGCATT AGCAGACAAC AGTTTGCAAC ATCCCCAGGA GAACACAGGA 
GACCTCCGAG CTTGCACATA TTGTAGAAAA ATAGCCTTAA GTTATGCTCA TTCCACAGAC 
AGTAATTCTA TTGGGGAAGA CTTGAATGCT CTTTCAGATT CTGCTTGCTC TGTGTCTGTG 
CTTGATCCAA GTGAACCCCG AACACCTGTT GGGAGTAGGA AAGCCAGCCG TAACATATTT 
TTAGAGGATG ATTTGGCCTG GCAAAGTTTG ATTCATCCAG ATTCCTCAAA TACTCCTCTT 
TCAACAAGAC TTGTATCTGT GCAAGAGGAT GCTGGGAAAT CTCCTGCTCG AAATAGATCA 
GCCAGCATTA CTAACCTGTC ACTGGATAGA TCTGGTTCTC CTATGGTACC TTCATATGAG 
ACATCTGTCA GTCCCCAGGC TAACCGAACA TATGTTAGGA CAGAGACCAC TGAGGATGAA 
CGCAAAATTC TTCTGGACAG TGTGCAGTTA AAAGACCTGT GGAAAAAAAT CTGCCATCAC 
AGCAGTGGAA TGGAGTTTCA GGATCACCGC TACTGGTTGA GAACGCATCC CAACTGCATT 
GTAGGAAAGG AATTAGTCAA CTGGCTAATC CGAAATGGGC ATATTGCCAC AAGGGCACAA 
GCTATAGCAA TTGGACAAGC AATGGTTGAT GGACGTTGGC TGGATTGTGT TAGTCATCAC 
GACCAGCTTT TCAGAGATGA GTATGCGCTG TATAGACCAC TGCAGAGTAC AGAATTTTCT 
GAGACGCCTT CTCCCGACAG TGACTCAGTG AACTCCGTGG AAGGACACTC TGAGCCATCC 
TGGTTTAAAG ACATAAAGTT TGATGACAGT GACACAGAAC AGATAGCTGA AGAAGGTGAC 
GATAATTTGG CTAATTCTGC CAGTCCTAGC AAGCGCACAT CAGTCAGCAG TTTCCAGTCC 
ACAGTGGACA GTGACTCAGC CGCTTCTATC AGCCTGAACG TGGAGCTGGA CAACGTGAAC 
TTCCATATCA AGAAGCCCTC CAAGTACCCA CATGTGCCCC CTCACCCTGC TGACCAAAAA 
GAGTATTTGA TTTCTGACAC TGGAGGACAA CAGCTCTCAA TAAGTGACGC TTTCATCAAA 
GAATCCTTAT TTAATCGCCG AGTAGAGGAA AAATCCAAAG AGCTGCCTTT CACACCTTTG 
GGCTGGCATC ATAACAACCT GGAGCTCCTG AGGGAGGAGA ATGGGGAGAA ACAAGCCATG 
GAGAGGTTGC TTTCAGCTAA TCATAACCAC ATGATGGCAC TACTCCAGCA GTTGCTCCAT 
AGTGACTCAC TGTCATCATC TTGGAGGGAC ATCATCGTGT CATTGGTCTG CCAGGTTGTT 
CAGACAGTCC GACCTGATGT CAAGAACCAG GATGATGACA TGGATATCCG TCAGTTTGTC 
CACATCAAAA AAATCCCAGG TGGAAAGAAG TTTGATTCTG TGGTTGTCAA TGGCTTTGTT 
TGTACCAAGA ACATTGCACA TAAAAAGATG AGTTCTTGTA TTAAAAACCC CAAAATTCTT 
CTGTTGAAGT GTTCCATTGA GTATCTCTAC AGAGAAGAAA CTAAGTTTAC TTGCATTGAT 
CCTATTGTGC TTCAGGAAAG GGAATTCTTG AAGAATTATG TCCAGCGAAT AGTTGATGTT 
CGACCCACCT TGGTTCTTGT TGAGAAAACA GTGTCTCGGA TTGCCCAGGA CATGTTATTG 
GAACATGGCA TTACTTTGGT CATTAATGTA AAGTCACAAG TTTTGGAACG AATCAGTCGA 
ATGACCCAAG GTGATTTAGT GATGTCAATG GACCAGCTGC TTACGAAACC ACACCTGGGC 
ACTTGTCACA AATTTTATAT GCAGATATTT CAGTTGCCTA ATGAACAAAC CAAGACACTG 
ATGTTTTTTG AAGGTTGTCC ACAGCACCTA GGCTGTACAA TCAAGCTAAG AGGAGGCTCT 
GATTATGAGC TGGCTCGAGT TAAGGAGATC CTAATATTTA TGATCTGTGT TGCTTATCAT 
TCTCAACTAG AAATATCCTT TCTCATGGAT GAATTTGCTA TGCCTCCCAC ATTAATGCAA 
AACCCTTCAT TCCATTCCCT GATTGAGGGA CGAGGGCATG AGGGGGCTGT CCAAGAGCAG 
TACGGTGGAG GTTCCATCCC CTGGGATCCT GACATCCCTC CTGAGTCTCT GCCCTGTGAT 
GATAGCAGTT TGCTGGAATT GAGGATTGTG TTTGAGAAGG GTGAGCAGGA AAATAAAAAT 
CTTCCGCAGG CTGTTGCCTC TGTGAAGCAT CAAGAACATA GCACAACAGC TTGCCCGGCG 
GGTCTCCCTT GTGCTTTCTT TGCACCTGTA CCGGAATCAT TGTTGCCACT CCCTGTGGAT 
GACCAACAAG ATGCTTTAGG CAGCGAGCAG CCAGAGACTT TGCAGCAAAC AGTTGTGCTG 
CAGGATCCCA AAAGCCAGAT AAGAGCCTTT AGAGACCCTC TACAGGATGA CACTGGATTA 
TATGTTACTG AGGAAGTCAC CTCCTCTGAA GATAAACGAA AGACTTATTC TTTGGCCTTT 
AAGCAGGAAT TAAAAGATGT GATCCTCTGT ATCTCCCCAG TAATCACATT CCGAGAACCC 
TTTCTTTTAA CTGAAAAGGG GATGAGATGC TCTACCCGAG ATTATTTTGC AGAGCAGGTT 
TACTGGTCTC CTCTCCTCAA TAAAGAATTC AAAGAAATGG AGAACAGGAG GAAGAAACAG 
CTGCTCAGGG ATCTCTCTGG ACTTCAGGGC ATGAATGGAA GTATTCAGGC CAAGTCTATT 
CAAGTCTTAC CCTCACATGA GCTAGTGAGC ACTAGAATTG CTGAGCATCT GGGCGATAGC 
CAGAGCTTGG GTAGAATGCT GGCCGATTAT CGAGCCAGAG GAGGAAGAAT TCAGCCCAAA 
AATTCAGACC CTTTTGCTCA TTCAAAGGAT GCATCAAGTA CTTCAAGTGG CCAATCAGGA 
AGCAAAAATG AGGGTGATGA AGAGAGAGGG CTTATTCTGA GTGATGCTGT GTGGTCAACA 
AAGGTGGACT GTCTGAATCC CATTAATCAC CAGAGACTTT GTGTGCTCTT CAGCAGCTCT 
TCTGCCCAGT CCAGCAATGC TCCTAGTGCC TGTGTCAGTC CTTGGATTGT AACAATGGAA 
TTTTATGGAA AGAATGATCT TACATTAGGA ATATTTTTAG AGAGATACTG TTTCAGGCCT 
TCTTATCAGT GTCCAAGCAT GTTCTGTGAT ACCCCCATGG TACATCATAT TCGGCGCTTT 
GTTCATGGCC AAGGCTGTGT GCAGATAATC CTGAAGGAGT TGGATTCTCC AGTACCTGGA 
TATCAGCATA CAATTCTTAC ATATTCCTGG TGTAGAATCT GCAAACAGGT AACACCAGTT 
GTTGCTCTTT CCAATGAGTC CTGGTCTATG TCATTTGCAA AATACCTTGA ACTTAGGTTT 
TATGGGCACC AGTATACTCG CAGAGCCAAC GCTGAGCCCT GTGGTCACTC CATCCATCAT 
GATTATCACC AGTATTTCTC CTATAACCAG ATGGTGGCGT CTTTCAGTTA TTCTCCCATT 
CGGCTTCTTG AAGTATGTGT TCCACTCCCC AAAATATTCA TTAAGCGTCA GGCCCCATTA 
AAAGTGTCCC TTCTTCAGGA TCTGAAGGAC TTCTTTCAAA AAGTTTCACA GGTATATGTT 
GCCATTGATG AAAGACTTGC ATCTTTGAAA ACTGATACAT TTAGTAAAAC AAGAGAGGAA 
AAAATGGAAG ATATTTTTGC ACAGAAAGAG ATGGAAGAAG GTGAGTTCAA GAACTGGATT 
GAGAAGATGC AAGCAAGGCT CATGTCTTCC TCTGTAGATA CCCCTCAGCA ACTGCAGTCG 
GTCTTTGAGT CACTCATTGC CAAGAAACAA AGTCTCTGTG AAGTGCTGCA AGCTTGGAAT 
AACAGGTTGC AGGACCTTTT CCAACAGGAA AAGGGTAGAA AGAGACCTTC AGTTCCTCCA 
AGTCCTGGAA GACTGAGACA AGGGGAAGAA AGCAAGATAA GTGCGATGGA TGCATCTCCA 
CGGAATATTT CTCCAGGACT TCAGAATGGA GAAAAAGAGG ATCGCTTCTT AACAACTTTG 
TCCAGCCAGA GCTCCACCAG TTCTACTCAT CTCCAATTGC CTACGCCACC TGAAGTCATG 
TCTGAACAGT CAGTGGGAGG GCCCCCTGAG CTAGATACAG CCAGCAGTTC CGAAGATGTG 
TTTGATGGGC ATTTGCTGGG ATCCACAGAC AGCCAAGTGA AGGAAAAGTC AACCATGAAA 
GCCATCTTTG CAAATTTGCT TCCAGGAAAT AGCTATAATC CTATTCCATT TCCTTTTGAT 
CCAGATAAAC ACTACTTAAT GTATGAACAT GAACGAGTGC CCATTGCAGT CTGCGAGAAG 
GAACCCAGCT CCATCATTGC TTTTGCTCTC AGTTGTAAAG AATACCGAAA TGCCTTAGAG 
GAATTGTCTA AAGCGACTCA GTGGAACAGT GCCGAAGAAG GGCTTCCAAC AAATAGTACT 
TCAGATAGCA GACCAAAGAG TAGCAGCCCT ATCAGATTAC CTGAAATGAG TGGAGGACAG 
ACAAATCGTA CAACAGAAAC AGAACCACAA CCAACCAAAA AGGCTTCTGG AATGTTGTCC 
TTCTTCAGAG GGACAGCAGG GAAAAGCCCC GATCTCTCTT CCCAGAAGAG AGAGACCTTA 
CGTGGAGCAG ATAGTGCTTA CTACCAGGTT GGGCAGACGG GCAAGGAGGG GACCGAGAAT 
CAAGGCGTTG AGCCTCAAGA TGAAGTAGAT GGAGGAGATA CACAAAAGAA GCAACTCATA 
AATCCTCATG TGGAACTTCA ATTTTCAGAT GCTAATGCCA AGTTTTACTG TCGGCTCTAC 
TATGCGGGAG AGTTTCATAA GATGCGTGAA GTGATTCTGG ACAGCAGTGA AGAAGATTTC 
ATTCGTTCCC TCTCCCACTC ATCACCCTGG CAGGCCCGGG GAGGCAAATC AGGAGCTGCC 
TTCTATGCAA CTGAGGATGA TAGATTTATT TTGAAGCAAA TGCCTCGTCT GGAAGTCCAG 
TCCTTCCTCG ACTTTGCACC ACATTACTTC AATTATATTA CAAATGCTGT TCAACAAAAG 
AGGCCCACGG CGTTGGCCAA AATTCTTGGA GTTTACAGAA TTGGTTATAA GAACTCTCAG 
AACAACACTG AGAAGAAGTT AGATCTCCTT GTCATGGAAA ATCTTTTCTA CGGGAGAAAG 
ATGGCACAGG TTTTTGATTT GAAGGGCTCT CTTAGGAATC GGAATGTAAA AACTGACACT 
GGAAAAGAGA GTTGTGATGT GGTCCTGCTA GATGAAAATC TCCTAAAGAT GGTTCGAGAC 
AACCCTCTAT ATATTCGTTC TCATTCCAAA GCTGTGCTGA GAACCTCGAT CCATAGTGAC 
TCCCATTTCC TTTCTAGCCA CCTCATTATA GATTATTCTT TGCTGGTTGG GCGAGATGAT 
ACTAGCAATG AGCTAGTAGT TGGAATTATA GATTATATTC GAACATTTAC ATGGGACAAA 
AAGCTTGAGA TGGTTGTGAA ATCAACAGGA ATTTTAGGTG GACAAGGTAA AATGCCAACA 
GTGGTGTCTC CGGAGTTGTA CAGGACTAGG TTTTGTGAGG CAATGGACAA GTATTTCCTA 
ATGGTACCAG ACCACTGGAC AGGCTTGGGT CTGAATTGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011509088.1
CDS	183..6224
Translation	MATDDKTSPTLDSANDLPRSPTSPSHLTHFKPLTPDQDEPPFKSAYSSFVNLFRFNKERAEGGQGEQQPLSGSWTSPQLPSRTQSVRSPTPYKKQLNEELQRRSSALADNSLQHPQENTGDLRACTYCRKIALSYAHSTDSNSIGEDLNALSDSACSVSVLDPSEPRTPVGSRKASRNIFLEDDLAWQSLIHPDSSNTPLSTRLVSVQEDAGKSPARNRSASITNLSLDRSGSPMVPSYETSVSPQANRTYVRTETTEDERKILLDSVQLKDLWKKICHHSSGMEFQDHRYWLRTHPNCIVGKELVNWLIRNGHIATRAQAIAIGQAMVDGRWLDCVSHHDQLFRDEYALYRPLQSTEFSETPSPDSDSVNSVEGHSEPSWFKDIKFDDSDTEQIAEEGDDNLANSASPSKRTSVSSFQSTVDSDSAASISLNVELDNVNFHIKKPSKYPHVPPHPADQKEYLISDTGGQQLSISDAFIKESLFNRRVEEKSKELPFTPLGWHHNNLELLREENGEKQAMERLLSANHNHMMALLQQLLHSDSLSSSWRDIIVSLVCQVVQTVRPDVKNQDDDMDIRQFVHIKKIPGGKKFDSVVVNGFVCTKNIAHKKMSSCIKNPKILLLKCSIEYLYREETKFTCIDPIVLQEREFLKNYVQRIVDVRPTLVLVEKTVSRIAQDMLLEHGITLVINVKSQVLERISRMTQGDLVMSMDQLLTKPHLGTCHKFYMQIFQLPNEQTKTLMFFEGCPQHLGCTIKLRGGSDYELARVKEILIFMICVAYHSQLEISFLMDEFAMPPTLMQNPSFHSLIEGRGHEGAVQEQYGGGSIPWDPDIPPESLPCDDSSLLELRIVFEKGEQENKNLPQAVASVKHQEHSTTACPAGLPCAFFAPVPESLLPLPVDDQQDALGSEQPETLQQTVVLQDPKSQIRAFRDPLQDDTGLYVTEEVTSSEDKRKTYSLAFKQELKDVILCISPVITFREPFLLTEKGMRCSTRDYFAEQVYWSPLLNKEFKEMENRRKKQLLRDLSGLQGMNGSIQAKSIQVLPSHELVSTRIAEHLGDSQSLGRMLADYRARGGRIQPKNSDPFAHSKDASSTSSGQSGSKNEGDEERGLILSDAVWSTKVDCLNPINHQRLCVLFSSSSAQSSNAPSACVSPWIVTMEFYGKNDLTLGIFLERYCFRPSYQCPSMFCDTPMVHHIRRFVHGQGCVQIILKELDSPVPGYQHTILTYSWCRICKQVTPVVALSNESWSMSFAKYLELRFYGHQYTRRANAEPCGHSIHHDYHQYFSYNQMVASFSYSPIRLLEVCVPLPKIFIKRQAPLKVSLLQDLKDFFQKVSQVYVAIDERLASLKTDTFSKTREEKMEDIFAQKEMEEGEFKNWIEKMQARLMSSSVDTPQQLQSVFESLIAKKQSLCEVLQAWNNRLQDLFQQEKGRKRPSVPPSPGRLRQGEESKISAMDASPRNISPGLQNGEKEDRFLTTLSSQSSTSSTHLQLPTPPEVMSEQSVGGPPELDTASSSEDVFDGHLLGSTDSQVKEKSTMKAIFANLLPGNSYNPIPFPFDPDKHYLMYEHERVPIAVCEKEPSSIIAFALSCKEYRNALEELSKATQWNSAEEGLPTNSTSDSRPKSSSPIRLPEMSGGQTNRTTETEPQPTKKASGMLSFFRGTAGKSPDLSSQKRETLRGADSAYYQVGQTGKEGTENQGVEPQDEVDGGDTQKKQLINPHVELQFSDANAKFYCRLYYAGEFHKMREVILDSSEEDFIRSLSHSSPWQARGGKSGAAFYATEDDRFILKQMPRLEVQSFLDFAPHYFNYITNAVQQKRPTALAKILGVYRIGYKNSQNNTEKKLDLLVMENLFYGRKMAQVFDLKGSLRNRNVKTDTGKESCDVVLLDENLLKMVRDNPLYIRSHSKAVLRTSIHSDSHFLSSHLIIDYSLLVGRDDTSNELVVGIIDYIRTFTWDKKLEMVVKSTGILGGQGKMPTVVSPELYRTRFCEAMDKYFLMVPDHWTGLGLNC

Target ORF information:

RefSeq Version	XM_011510786.3
Organism	Homo sapiens(human)
Definition	Homo sapiens phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011510786.3

ORF Insert Sequence:

ATGGCCACAG ATGATAAGAC GTCCCCAACA CTGGACTCTG CTAATGATTT GCCTCGATCT 
CCTACTAGTC CTTCTCATCT CACACACTTT AAACCTTTGA CTCCTGATCA AGATGAGCCC 
CCTTTTAAAT CAGCTTATAG TTCTTTTGTA AATCTCTTTC GTTTTAACAA AGAGAGAGCA 
GAAGGAGGCC AGGGAGAACA GCAGCCTTTG AGTGGAAGTT GGACCAGCCC TCAGCTCCCT 
TCGAGGACAC AGTCTGTTAG GTCACCCACA CCTTATAAAA AGCAGCTTAA TGAGGAACTC 
CAGCGGCGCT CTTCAGCATT AGCAGACAAC AGTTTGCAAC ATCCCCAGGA GAACACAGGA 
GACCTCCGAG CTTGCACATA TTGTAGAAAA ATAGCCTTAA GTTATGCTCA TTCCACAGAC 
AGTAATTCTA TTGGGGAAGA CTTGAATGCT CTTTCAGATT CTGCTTGCTC TGTGTCTGTG 
CTTGATCCAA GTGAACCCCG AACACCTGTT GGGAGTAGGA AAGCCAGCCG TAACATATTT 
TTAGAGGATG ATTTGGCCTG GCAAAGTTTG ATTCATCCAG ATTCCTCAAA TACTCCTCTT 
TCAACAAGAC TTGTATCTGT GCAAGAGGAT GCTGGGAAAT CTCCTGCTCG AAATAGATCA 
GCCAGCATTA CTAACCTGTC ACTGGATAGA TCTGGTTCTC CTATGGTACC TTCATATGAG 
ACATCTGTCA GTCCCCAGGC TAACCGAACA TATGTTAGGA CAGAGACCAC TGAGGATGAA 
CGCAAAATTC TTCTGGACAG TGTGCAGTTA AAAGACCTGT GGAAAAAAAT CTGCCATCAC 
AGCAGTGGAA TGGAGTTTCA GGATCACCGC TACTGGTTGA GAACGCATCC CAACTGCATT 
GTAGGAAAGG AATTAGTCAA CTGGCTAATC CGAAATGGGC ATATTGCCAC AAGGGCACAA 
GCTATAGCAA TTGGACAAGC AATGGTTGAT GGACGTTGGC TGGATTGTGT TAGTCATCAC 
GACCAGCTTT TCAGAGATGA GTATGCGCTG TATAGACCAC TGCAGAGTAC AGAATTTTCT 
GAGACGCCTT CTCCCGACAG TGACTCAGTG AACTCCGTGG AAGGACACTC TGAGCCATCC 
TGGTTTAAAG ACATAAAGTT TGATGACAGT GACACAGAAC AGATAGCTGA AGAAGGTGAC 
GATAATTTGG CTAATTCTGC CAGTCCTAGC AAGCGCACAT CAGTCAGCAG TTTCCAGTCC 
ACAGTGGACA GTGACTCAGC CGCTTCTATC AGCCTGAACG TGGAGCTGGA CAACGTGAAC 
TTCCATATCA AGAAGCCCTC CAAGTACCCA CATGTGCCCC CTCACCCTGC TGACCAAAAA 
GAGTATTTGA TTTCTGACAC TGGAGGACAA CAGCTCTCAA TAAGTGACGC TTTCATCAAA 
GAATCCTTAT TTAATCGCCG AGTAGAGGAA AAATCCAAAG AGCTGCCTTT CACACCTTTG 
GGCTGGCATC ATAACAACCT GGAGCTCCTG AGGGAGGAGA ATGGGGAGAA ACAAGCCATG 
GAGAGGTTGC TTTCAGCTAA TCATAACCAC ATGATGGCAC TACTCCAGCA GTTGCTCCAT 
AGTGACTCAC TGTCATCATC TTGGAGGGAC ATCATCGTGT CATTGGTCTG CCAGGTTGTT 
CAGACAGTCC GACCTGATGT CAAGAACCAG GATGATGACA TGGATATCCG TCAGTTTGTC 
CACATCAAAA AAATCCCAGG TGGAAAGAAG TTTGATTCTG TGGTTGTCAA TGGCTTTGTT 
TGTACCAAGA ACATTGCACA TAAAAAGATG AGTTCTTGTA TTAAAAACCC CAAAATTCTT 
CTGTTGAAGT GTTCCATTGA GTATCTCTAC AGAGAAGAAA CTAAGTTTAC TTGCATTGAT 
CCTATTGTGC TTCAGGAAAG GGAATTCTTG AAGAATTATG TCCAGCGAAT AGTTGATGTT 
CGACCCACCT TGGTTCTTGT TGAGAAAACA GTGTCTCGGA TTGCCCAGGA CATGTTATTG 
GAACATGGCA TTACTTTGGT CATTAATGTA AAGTCACAAG TTTTGGAACG AATCAGTCGA 
ATGACCCAAG GTGATTTAGT GATGTCAATG GACCAGCTGC TTACGAAACC ACACCTGGGC 
ACTTGTCACA AATTTTATAT GCAGATATTT CAGTTGCCTA ATGAACAAAC CAAGACACTG 
ATGTTTTTTG AAGGTTGTCC ACAGCACCTA GGCTGTACAA TCAAGCTAAG AGGAGGCTCT 
GATTATGAGC TGGCTCGAGT TAAGGAGATC CTAATATTTA TGATCTGTGT TGCTTATCAT 
TCTCAACTAG AAATATCCTT TCTCATGGAT GAATTTGCTA TGCCTCCCAC ATTAATGCAA 
AACCCTTCAT TCCATTCCCT GATTGAGGGA CGAGGGCATG AGGGGGCTGT CCAAGAGCAG 
TACGGTGGAG GTTCCATCCC CTGGGATCCT GACATCCCTC CTGAGTCTCT GCCCTGTGAT 
GATAGCAGTT TGCTGGAATT GAGGATTGTG TTTGAGAAGG GTGAGCAGGA AAATAAAAAT 
CTTCCGCAGG CTGTTGCCTC TGTGAAGCAT CAAGAACATA GCACAACAGC TTGCCCGGCG 
GGTCTCCCTT GTGCTTTCTT TGCACCTGTA CCGGAATCAT TGTTGCCACT CCCTGTGGAT 
GACCAACAAG ATGCTTTAGG CAGCGAGCAG CCAGAGACTT TGCAGCAAAC AGTTGTGCTG 
CAGGATCCCA AAAGCCAGAT AAGAGCCTTT AGAGACCCTC TACAGGATGA CACTGGATTA 
TATGTTACTG AGGAAGTCAC CTCCTCTGAA GATAAACGAA AGACTTATTC TTTGGCCTTT 
AAGCAGGAAT TAAAAGATGT GATCCTCTGT ATCTCCCCAG TAATCACATT CCGAGAACCC 
TTTCTTTTAA CTGAAAAGGG GATGAGATGC TCTACCCGAG ATTATTTTGC AGAGCAGGTT 
TACTGGTCTC CTCTCCTCAA TAAAGAATTC AAAGAAATGG AGAACAGGAG GAAGAAACAG 
CTGCTCAGGG ATCTCTCTGG ACTTCAGGGC ATGAATGGAA GTATTCAGGC CAAGTCTATT 
CAAGTCTTAC CCTCACATGA GCTAGTGAGC ACTAGAATTG CTGAGCATCT GGGCGATAGC 
CAGAGCTTGG GTAGAATGCT GGCCGATTAT CGAGCCAGAG GAGGAAGAAT TCAGCCCAAA 
AATTCAGACC CTTTTGCTCA TTCAAAGGAT GCATCAAGTA CTTCAAGTGG CCAATCAGGA 
AGCAAAAATG AGGGTGATGA AGAGAGAGGG CTTATTCTGA GTGATGCTGT GTGGTCAACA 
AAGGTGGACT GTCTGAATCC CATTAATCAC CAGAGACTTT GTGTGCTCTT CAGCAGCTCT 
TCTGCCCAGT CCAGCAATGC TCCTAGTGCC TGTGTCAGTC CTTGGATTGT AACAATGGAA 
TTTTATGGAA AGAATGATCT TACATTAGGA ATATTTTTAG AGAGATACTG TTTCAGGCCT 
TCTTATCAGT GTCCAAGCAT GTTCTGTGAT ACCCCCATGG TACATCATAT TCGGCGCTTT 
GTTCATGGCC AAGGCTGTGT GCAGATAATC CTGAAGGAGT TGGATTCTCC AGTACCTGGA 
TATCAGCATA CAATTCTTAC ATATTCCTGG TGTAGAATCT GCAAACAGGT AACACCAGTT 
GTTGCTCTTT CCAATGAGTC CTGGTCTATG TCATTTGCAA AATACCTTGA ACTTAGGTTT 
TATGGGCACC AGTATACTCG CAGAGCCAAC GCTGAGCCCT GTGGTCACTC CATCCATCAT 
GATTATCACC AGTATTTCTC CTATAACCAG ATGGTGGCGT CTTTCAGTTA TTCTCCCATT 
CGGCTTCTTG AAGTATGTGT TCCACTCCCC AAAATATTCA TTAAGCGTCA GGCCCCATTA 
AAAGTGTCCC TTCTTCAGGA TCTGAAGGAC TTCTTTCAAA AAGTTTCACA GGTATATGTT 
GCCATTGATG AAAGACTTGC ATCTTTGAAA ACTGATACAT TTAGTAAAAC AAGAGAGGAA 
AAAATGGAAG ATATTTTTGC ACAGAAAGAG ATGGAAGAAG GTGAGTTCAA GAACTGGATT 
GAGAAGATGC AAGCAAGGCT CATGTCTTCC TCTGTAGATA CCCCTCAGCA ACTGCAGTCG 
GTCTTTGAGT CACTCATTGC CAAGAAACAA AGTCTCTGTG AAGTGCTGCA AGCTTGGAAT 
AACAGGTTGC AGGACCTTTT CCAACAGGAA AAGGGTAGAA AGAGACCTTC AGTTCCTCCA 
AGTCCTGGAA GACTGAGACA AGGGGAAGAA AGCAAGATAA GTGCGATGGA TGCATCTCCA 
CGGAATATTT CTCCAGGACT TCAGAATGGA GAAAAAGAGG ATCGCTTCTT AACAACTTTG 
TCCAGCCAGA GCTCCACCAG TTCTACTCAT CTCCAATTGC CTACGCCACC TGAAGTCATG 
TCTGAACAGT CAGTGGGAGG GCCCCCTGAG CTAGATACAG CCAGCAGTTC CGAAGATGTG 
TTTGATGGGC ATTTGCTGGG ATCCACAGAC AGCCAAGTGA AGGAAAAGTC AACCATGAAA 
GCCATCTTTG CAAATTTGCT TCCAGGAAAT AGCTATAATC CTATTCCATT TCCTTTTGAT 
CCAGATAAAC ACTACTTAAT GTATGAACAT GAACGAGTGC CCATTGCAGT CTGCGAGAAG 
GAACCCAGCT CCATCATTGC TTTTGCTCTC AGTTGTAAAG AATACCGAAA TGCCTTAGAG 
GAATTGTCTA AAGCGACTCA GTGGAACAGT GCCGAAGAAG GGCTTCCAAC AAATAGTACT 
TCAGATAGCA GACCAAAGAG TAGCAGCCCT ATCAGATTAC CTGAAATGAG TGGAGGACAG 
ACAAATCGTA CAACAGAAAC AGAACCACAA CCAACCAAAA AGGCTTCTGG AATGTTGTCC 
TTCTTCAGAG GGACAGCAGG GAAAAGCCCC GATCTCTCTT CCCAGAAGAG AGAGACCTTA 
CGTGGAGCAG ATAGTGCTTA CTACCAGGTT GGGCAGACGG GCAAGGAGGG GACCGAGAAT 
CAAGGCGTTG AGCCTCAAGA TGAAGTAGAT GGAGGAGATA CACAAAAGAA GCAACTCATA 
AATCCTCATG TGGAACTTCA ATTTTCAGAT GCTAATGCCA AGTTTTACTG TCGGCTCTAC 
TATGCGGGAG AGTTTCATAA GATGCGTGAA GTGATTCTGG ACAGCAGTGA AGAAGATTTC 
ATTCGTTCCC TCTCCCACTC ATCACCCTGG CAGGCCCGGG GAGGCAAATC AGGAGCTGCC 
TTCTATGCAA CTGAGGATGA TAGATTTATT TTGAAGCAAA TGCCTCGTCT GGAAGTCCAG 
TCCTTCCTCG ACTTTGCACC ACATTACTTC AATTATATTA CAAATGCTGT TCAACAAAAG 
AGGCCCACGG CGTTGGCCAA AATTCTTGGA GTTTACAGAA TTGGTTATAA GAACTCTCAG 
AACAACACTG AGAAGAAGTT AGATCTCCTT GTCATGGAAA ATCTTTTCTA CGGGAGAAAG 
ATGGCACAGG TTTTTGATTT GAAGGGCTCT CTTAGGAATC GGAATGTAAA AACTGACACT 
GGAAAAGAGA GTTGTGATGT GGTCCTGCTA GATGAAAATC TCCTAAAGAT GGTTCGAGAC 
AACCCTCTAT ATATTCGTTC TCATTCCAAA GCTGTGCTGA GAACCTCGAT CCATAGTGAC 
TCCCATTTCC TTTCTAGCCA CCTCATTATA GATTATTCTT TGCTGGTTGG GCGAGATGAT 
ACTAGCAATG AGCTAGTAGT TGGAATTATA GATTATATTC GAACATTTAC ATGGGACAAA 
AAGCTTGAGA TGGTTGTGAA ATCAACAGGA ATTTTAGGTG GACAAGGTAA AATGCCAACA 
GTGGTGTCTC CGGAGTTGTA CAGGACTAGG TTTTGTGAGG CAATGGACAA GTATTTCCTA 
ATGGTACCAG ACCACTGGAC AGGCTTGGGT CTGAATTGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PIKFYVE ( XM_011510787.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011509089.1 Homo sapiens phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X12, mRNA.

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CloneID	OHu77669
Clone ID Related Accession (Same CDS sequence)	XM_011510787.1
Accession Version	XM_011510787.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6039bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	1-phosphatidylinositol 3-phosphate 5-kinase isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG ATGATAAGAC GTCCCCAACA CTGGACTCTG CTAATGATTT GCCTCGATCT 
CCTACTAGTC CTTCTCATCT CACACACTTT AAACCTTTGA CTCCTGATCA AGATGAGCCC 
CCTTTTAAAT CAGCTTATAG TTCTTTTGTA AATCTCTTTC GTTTTAACAA AGAGAGAGCA 
GAAGGAGGCC AGGGAGAACA GCAGCCTTTG AGTGGAAGTT GGACCAGCCC TCAGCTCCCT 
TCGAGGACAC AGTCTGTTAG GTCACCCACA CCTTATAAAA AGCAGCTTAA TGAGGAACTC 
CAGCGGCGCT CTTCAGCATT AGACAACAGT TTGCAACATC CCCAGGAGAA CACAGGAGAC 
CTCCGAGCTT GCACATATTG TAGAAAAATA GCCTTAAGTT ATGCTCATTC CACAGACAGT 
AATTCTATTG GGGAAGACTT GAATGCTCTT TCAGATTCTG CTTGCTCTGT GTCTGTGCTT 
GATCCAAGTG AACCCCGAAC ACCTGTTGGG AGTAGGAAAG CCAGCCGTAA CATATTTTTA 
GAGGATGATT TGGCCTGGCA AAGTTTGATT CATCCAGATT CCTCAAATAC TCCTCTTTCA 
ACAAGACTTG TATCTGTGCA AGAGGATGCT GGGAAATCTC CTGCTCGAAA TAGATCAGCC 
AGCATTACTA ACCTGTCACT GGATAGATCT GGTTCTCCTA TGGTACCTTC ATATGAGACA 
TCTGTCAGTC CCCAGGCTAA CCGAACATAT GTTAGGACAG AGACCACTGA GGATGAACGC 
AAAATTCTTC TGGACAGTGT GCAGTTAAAA GACCTGTGGA AAAAAATCTG CCATCACAGC 
AGTGGAATGG AGTTTCAGGA TCACCGCTAC TGGTTGAGAA CGCATCCCAA CTGCATTGTA 
GGAAAGGAAT TAGTCAACTG GCTAATCCGA AATGGGCATA TTGCCACAAG GGCACAAGCT 
ATAGCAATTG GACAAGCAAT GGTTGATGGA CGTTGGCTGG ATTGTGTTAG TCATCACGAC 
CAGCTTTTCA GAGATGAGTA TGCGCTGTAT AGACCACTGC AGAGTACAGA ATTTTCTGAG 
ACGCCTTCTC CCGACAGTGA CTCAGTGAAC TCCGTGGAAG GACACTCTGA GCCATCCTGG 
TTTAAAGACA TAAAGTTTGA TGACAGTGAC ACAGAACAGA TAGCTGAAGA AGGTGACGAT 
AATTTGGCTA ATTCTGCCAG TCCTAGCAAG CGCACATCAG TCAGCAGTTT CCAGTCCACA 
GTGGACAGTG ACTCAGCCGC TTCTATCAGC CTGAACGTGG AGCTGGACAA CGTGAACTTC 
CATATCAAGA AGCCCTCCAA GTACCCACAT GTGCCCCCTC ACCCTGCTGA CCAAAAAGAG 
TATTTGATTT CTGACACTGG AGGACAACAG CTCTCAATAA GTGACGCTTT CATCAAAGAA 
TCCTTATTTA ATCGCCGAGT AGAGGAAAAA TCCAAAGAGC TGCCTTTCAC ACCTTTGGGC 
TGGCATCATA ACAACCTGGA GCTCCTGAGG GAGGAGAATG GGGAGAAACA AGCCATGGAG 
AGGTTGCTTT CAGCTAATCA TAACCACATG ATGGCACTAC TCCAGCAGTT GCTCCATAGT 
GACTCACTGT CATCATCTTG GAGGGACATC ATCGTGTCAT TGGTCTGCCA GGTTGTTCAG 
ACAGTCCGAC CTGATGTCAA GAACCAGGAT GATGACATGG ATATCCGTCA GTTTGTCCAC 
ATCAAAAAAA TCCCAGGTGG AAAGAAGTTT GATTCTGTGG TTGTCAATGG CTTTGTTTGT 
ACCAAGAACA TTGCACATAA AAAGATGAGT TCTTGTATTA AAAACCCCAA AATTCTTCTG 
TTGAAGTGTT CCATTGAGTA TCTCTACAGA GAAGAAACTA AGTTTACTTG CATTGATCCT 
ATTGTGCTTC AGGAAAGGGA ATTCTTGAAG AATTATGTCC AGCGAATAGT TGATGTTCGA 
CCCACCTTGG TTCTTGTTGA GAAAACAGTG TCTCGGATTG CCCAGGACAT GTTATTGGAA 
CATGGCATTA CTTTGGTCAT TAATGTAAAG TCACAAGTTT TGGAACGAAT CAGTCGAATG 
ACCCAAGGTG ATTTAGTGAT GTCAATGGAC CAGCTGCTTA CGAAACCACA CCTGGGCACT 
TGTCACAAAT TTTATATGCA GATATTTCAG TTGCCTAATG AACAAACCAA GACACTGATG 
TTTTTTGAAG GTTGTCCACA GCACCTAGGC TGTACAATCA AGCTAAGAGG AGGCTCTGAT 
TATGAGCTGG CTCGAGTTAA GGAGATCCTA ATATTTATGA TCTGTGTTGC TTATCATTCT 
CAACTAGAAA TATCCTTTCT CATGGATGAA TTTGCTATGC CTCCCACATT AATGCAAAAC 
CCTTCATTCC ATTCCCTGAT TGAGGGACGA GGGCATGAGG GGGCTGTCCA AGAGCAGTAC 
GGTGGAGGTT CCATCCCCTG GGATCCTGAC ATCCCTCCTG AGTCTCTGCC CTGTGATGAT 
AGCAGTTTGC TGGAATTGAG GATTGTGTTT GAGAAGGGTG AGCAGGAAAA TAAAAATCTT 
CCGCAGGCTG TTGCCTCTGT GAAGCATCAA GAACATAGCA CAACAGCTTG CCCGGCGGGT 
CTCCCTTGTG CTTTCTTTGC ACCTGTACCG GAATCATTGT TGCCACTCCC TGTGGATGAC 
CAACAAGATG CTTTAGGCAG CGAGCAGCCA GAGACTTTGC AGCAAACAGT TGTGCTGCAG 
GATCCCAAAA GCCAGATAAG AGCCTTTAGA GACCCTCTAC AGGATGACAC TGGATTATAT 
GTTACTGAGG AAGTCACCTC CTCTGAAGAT AAACGAAAGA CTTATTCTTT GGCCTTTAAG 
CAGGAATTAA AAGATGTGAT CCTCTGTATC TCCCCAGTAA TCACATTCCG AGAACCCTTT 
CTTTTAACTG AAAAGGGGAT GAGATGCTCT ACCCGAGATT ATTTTGCAGA GCAGGTTTAC 
TGGTCTCCTC TCCTCAATAA AGAATTCAAA GAAATGGAGA ACAGGAGGAA GAAACAGCTG 
CTCAGGGATC TCTCTGGACT TCAGGGCATG AATGGAAGTA TTCAGGCCAA GTCTATTCAA 
GTCTTACCCT CACATGAGCT AGTGAGCACT AGAATTGCTG AGCATCTGGG CGATAGCCAG 
AGCTTGGGTA GAATGCTGGC CGATTATCGA GCCAGAGGAG GAAGAATTCA GCCCAAAAAT 
TCAGACCCTT TTGCTCATTC AAAGGATGCA TCAAGTACTT CAAGTGGCCA ATCAGGAAGC 
AAAAATGAGG GTGATGAAGA GAGAGGGCTT ATTCTGAGTG ATGCTGTGTG GTCAACAAAG 
GTGGACTGTC TGAATCCCAT TAATCACCAG AGACTTTGTG TGCTCTTCAG CAGCTCTTCT 
GCCCAGTCCA GCAATGCTCC TAGTGCCTGT GTCAGTCCTT GGATTGTAAC AATGGAATTT 
TATGGAAAGA ATGATCTTAC ATTAGGAATA TTTTTAGAGA GATACTGTTT CAGGCCTTCT 
TATCAGTGTC CAAGCATGTT CTGTGATACC CCCATGGTAC ATCATATTCG GCGCTTTGTT 
CATGGCCAAG GCTGTGTGCA GATAATCCTG AAGGAGTTGG ATTCTCCAGT ACCTGGATAT 
CAGCATACAA TTCTTACATA TTCCTGGTGT AGAATCTGCA AACAGGTAAC ACCAGTTGTT 
GCTCTTTCCA ATGAGTCCTG GTCTATGTCA TTTGCAAAAT ACCTTGAACT TAGGTTTTAT 
GGGCACCAGT ATACTCGCAG AGCCAACGCT GAGCCCTGTG GTCACTCCAT CCATCATGAT 
TATCACCAGT ATTTCTCCTA TAACCAGATG GTGGCGTCTT TCAGTTATTC TCCCATTCGG 
CTTCTTGAAG TATGTGTTCC ACTCCCCAAA ATATTCATTA AGCGTCAGGC CCCATTAAAA 
GTGTCCCTTC TTCAGGATCT GAAGGACTTC TTTCAAAAAG TTTCACAGGT ATATGTTGCC 
ATTGATGAAA GACTTGCATC TTTGAAAACT GATACATTTA GTAAAACAAG AGAGGAAAAA 
ATGGAAGATA TTTTTGCACA GAAAGAGATG GAAGAAGGTG AGTTCAAGAA CTGGATTGAG 
AAGATGCAAG CAAGGCTCAT GTCTTCCTCT GTAGATACCC CTCAGCAACT GCAGTCGGTC 
TTTGAGTCAC TCATTGCCAA GAAACAAAGT CTCTGTGAAG TGCTGCAAGC TTGGAATAAC 
AGGTTGCAGG ACCTTTTCCA ACAGGAAAAG GGTAGAAAGA GACCTTCAGT TCCTCCAAGT 
CCTGGAAGAC TGAGACAAGG GGAAGAAAGC AAGATAAGTG CGATGGATGC ATCTCCACGG 
AATATTTCTC CAGGACTTCA GAATGGAGAA AAAGAGGATC GCTTCTTAAC AACTTTGTCC 
AGCCAGAGCT CCACCAGTTC TACTCATCTC CAATTGCCTA CGCCACCTGA AGTCATGTCT 
GAACAGTCAG TGGGAGGGCC CCCTGAGCTA GATACAGCCA GCAGTTCCGA AGATGTGTTT 
GATGGGCATT TGCTGGGATC CACAGACAGC CAAGTGAAGG AAAAGTCAAC CATGAAAGCC 
ATCTTTGCAA ATTTGCTTCC AGGAAATAGC TATAATCCTA TTCCATTTCC TTTTGATCCA 
GATAAACACT ACTTAATGTA TGAACATGAA CGAGTGCCCA TTGCAGTCTG CGAGAAGGAA 
CCCAGCTCCA TCATTGCTTT TGCTCTCAGT TGTAAAGAAT ACCGAAATGC CTTAGAGGAA 
TTGTCTAAAG CGACTCAGTG GAACAGTGCC GAAGAAGGGC TTCCAACAAA TAGTACTTCA 
GATAGCAGAC CAAAGAGTAG CAGCCCTATC AGATTACCTG AAATGAGTGG AGGACAGACA 
AATCGTACAA CAGAAACAGA ACCACAACCA ACCAAAAAGG CTTCTGGAAT GTTGTCCTTC 
TTCAGAGGGA CAGCAGGGAA AAGCCCCGAT CTCTCTTCCC AGAAGAGAGA GACCTTACGT 
GGAGCAGATA GTGCTTACTA CCAGGTTGGG CAGACGGGCA AGGAGGGGAC CGAGAATCAA 
GGCGTTGAGC CTCAAGATGA AGTAGATGGA GGAGATACAC AAAAGAAGCA ACTCATAAAT 
CCTCATGTGG AACTTCAATT TTCAGATGCT AATGCCAAGT TTTACTGTCG GCTCTACTAT 
GCGGGAGAGT TTCATAAGAT GCGTGAAGTG ATTCTGGACA GCAGTGAAGA AGATTTCATT 
CGTTCCCTCT CCCACTCATC ACCCTGGCAG GCCCGGGGAG GCAAATCAGG AGCTGCCTTC 
TATGCAACTG AGGATGATAG ATTTATTTTG AAGCAAATGC CTCGTCTGGA AGTCCAGTCC 
TTCCTCGACT TTGCACCACA TTACTTCAAT TATATTACAA ATGCTGTTCA ACAAAAGAGG 
CCCACGGCGT TGGCCAAAAT TCTTGGAGTT TACAGAATTG GTTATAAGAA CTCTCAGAAC 
AACACTGAGA AGAAGTTAGA TCTCCTTGTC ATGGAAAATC TTTTCTACGG GAGAAAGATG 
GCACAGGTTT TTGATTTGAA GGGCTCTCTT AGGAATCGGA ATGTAAAAAC TGACACTGGA 
AAAGAGAGTT GTGATGTGGT CCTGCTAGAT GAAAATCTCC TAAAGATGGT TCGAGACAAC 
CCTCTATATA TTCGTTCTCA TTCCAAAGCT GTGCTGAGAA CCTCGATCCA TAGTGACTCC 
CATTTCCTTT CTAGCCACCT CATTATAGAT TATTCTTTGC TGGTTGGGCG AGATGATACT 
AGCAATGAGC TAGTAGTTGG AATTATAGAT TATATTCGAA CATTTACATG GGACAAAAAG 
CTTGAGATGG TTGTGAAATC AACAGGAATT TTAGGTGGAC AAGGTAAAAT GCCAACAGTG 
GTGTCTCCGG AGTTGTACAG GACTAGGTTT TGTGAGGCAA TGGACAAGTA TTTCCTAATG 
GTACCAGACC ACTGGACAGG CTTGGGTCTG AATTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011509089.1
CDS	143..6181
Translation	MATDDKTSPTLDSANDLPRSPTSPSHLTHFKPLTPDQDEPPFKSAYSSFVNLFRFNKERAEGGQGEQQPLSGSWTSPQLPSRTQSVRSPTPYKKQLNEELQRRSSALDNSLQHPQENTGDLRACTYCRKIALSYAHSTDSNSIGEDLNALSDSACSVSVLDPSEPRTPVGSRKASRNIFLEDDLAWQSLIHPDSSNTPLSTRLVSVQEDAGKSPARNRSASITNLSLDRSGSPMVPSYETSVSPQANRTYVRTETTEDERKILLDSVQLKDLWKKICHHSSGMEFQDHRYWLRTHPNCIVGKELVNWLIRNGHIATRAQAIAIGQAMVDGRWLDCVSHHDQLFRDEYALYRPLQSTEFSETPSPDSDSVNSVEGHSEPSWFKDIKFDDSDTEQIAEEGDDNLANSASPSKRTSVSSFQSTVDSDSAASISLNVELDNVNFHIKKPSKYPHVPPHPADQKEYLISDTGGQQLSISDAFIKESLFNRRVEEKSKELPFTPLGWHHNNLELLREENGEKQAMERLLSANHNHMMALLQQLLHSDSLSSSWRDIIVSLVCQVVQTVRPDVKNQDDDMDIRQFVHIKKIPGGKKFDSVVVNGFVCTKNIAHKKMSSCIKNPKILLLKCSIEYLYREETKFTCIDPIVLQEREFLKNYVQRIVDVRPTLVLVEKTVSRIAQDMLLEHGITLVINVKSQVLERISRMTQGDLVMSMDQLLTKPHLGTCHKFYMQIFQLPNEQTKTLMFFEGCPQHLGCTIKLRGGSDYELARVKEILIFMICVAYHSQLEISFLMDEFAMPPTLMQNPSFHSLIEGRGHEGAVQEQYGGGSIPWDPDIPPESLPCDDSSLLELRIVFEKGEQENKNLPQAVASVKHQEHSTTACPAGLPCAFFAPVPESLLPLPVDDQQDALGSEQPETLQQTVVLQDPKSQIRAFRDPLQDDTGLYVTEEVTSSEDKRKTYSLAFKQELKDVILCISPVITFREPFLLTEKGMRCSTRDYFAEQVYWSPLLNKEFKEMENRRKKQLLRDLSGLQGMNGSIQAKSIQVLPSHELVSTRIAEHLGDSQSLGRMLADYRARGGRIQPKNSDPFAHSKDASSTSSGQSGSKNEGDEERGLILSDAVWSTKVDCLNPINHQRLCVLFSSSSAQSSNAPSACVSPWIVTMEFYGKNDLTLGIFLERYCFRPSYQCPSMFCDTPMVHHIRRFVHGQGCVQIILKELDSPVPGYQHTILTYSWCRICKQVTPVVALSNESWSMSFAKYLELRFYGHQYTRRANAEPCGHSIHHDYHQYFSYNQMVASFSYSPIRLLEVCVPLPKIFIKRQAPLKVSLLQDLKDFFQKVSQVYVAIDERLASLKTDTFSKTREEKMEDIFAQKEMEEGEFKNWIEKMQARLMSSSVDTPQQLQSVFESLIAKKQSLCEVLQAWNNRLQDLFQQEKGRKRPSVPPSPGRLRQGEESKISAMDASPRNISPGLQNGEKEDRFLTTLSSQSSTSSTHLQLPTPPEVMSEQSVGGPPELDTASSSEDVFDGHLLGSTDSQVKEKSTMKAIFANLLPGNSYNPIPFPFDPDKHYLMYEHERVPIAVCEKEPSSIIAFALSCKEYRNALEELSKATQWNSAEEGLPTNSTSDSRPKSSSPIRLPEMSGGQTNRTTETEPQPTKKASGMLSFFRGTAGKSPDLSSQKRETLRGADSAYYQVGQTGKEGTENQGVEPQDEVDGGDTQKKQLINPHVELQFSDANAKFYCRLYYAGEFHKMREVILDSSEEDFIRSLSHSSPWQARGGKSGAAFYATEDDRFILKQMPRLEVQSFLDFAPHYFNYITNAVQQKRPTALAKILGVYRIGYKNSQNNTEKKLDLLVMENLFYGRKMAQVFDLKGSLRNRNVKTDTGKESCDVVLLDENLLKMVRDNPLYIRSHSKAVLRTSIHSDSHFLSSHLIIDYSLLVGRDDTSNELVVGIIDYIRTFTWDKKLEMVVKSTGILGGQGKMPTVVSPELYRTRFCEAMDKYFLMVPDHWTGLGLNC

Target ORF information:

RefSeq Version	XM_011510787.1
Organism	Homo sapiens(human)
Definition	Homo sapiens phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011510787.1

ORF Insert Sequence:

ATGGCCACAG ATGATAAGAC GTCCCCAACA CTGGACTCTG CTAATGATTT GCCTCGATCT 
CCTACTAGTC CTTCTCATCT CACACACTTT AAACCTTTGA CTCCTGATCA AGATGAGCCC 
CCTTTTAAAT CAGCTTATAG TTCTTTTGTA AATCTCTTTC GTTTTAACAA AGAGAGAGCA 
GAAGGAGGCC AGGGAGAACA GCAGCCTTTG AGTGGAAGTT GGACCAGCCC TCAGCTCCCT 
TCGAGGACAC AGTCTGTTAG GTCACCCACA CCTTATAAAA AGCAGCTTAA TGAGGAACTC 
CAGCGGCGCT CTTCAGCATT AGACAACAGT TTGCAACATC CCCAGGAGAA CACAGGAGAC 
CTCCGAGCTT GCACATATTG TAGAAAAATA GCCTTAAGTT ATGCTCATTC CACAGACAGT 
AATTCTATTG GGGAAGACTT GAATGCTCTT TCAGATTCTG CTTGCTCTGT GTCTGTGCTT 
GATCCAAGTG AACCCCGAAC ACCTGTTGGG AGTAGGAAAG CCAGCCGTAA CATATTTTTA 
GAGGATGATT TGGCCTGGCA AAGTTTGATT CATCCAGATT CCTCAAATAC TCCTCTTTCA 
ACAAGACTTG TATCTGTGCA AGAGGATGCT GGGAAATCTC CTGCTCGAAA TAGATCAGCC 
AGCATTACTA ACCTGTCACT GGATAGATCT GGTTCTCCTA TGGTACCTTC ATATGAGACA 
TCTGTCAGTC CCCAGGCTAA CCGAACATAT GTTAGGACAG AGACCACTGA GGATGAACGC 
AAAATTCTTC TGGACAGTGT GCAGTTAAAA GACCTGTGGA AAAAAATCTG CCATCACAGC 
AGTGGAATGG AGTTTCAGGA TCACCGCTAC TGGTTGAGAA CGCATCCCAA CTGCATTGTA 
GGAAAGGAAT TAGTCAACTG GCTAATCCGA AATGGGCATA TTGCCACAAG GGCACAAGCT 
ATAGCAATTG GACAAGCAAT GGTTGATGGA CGTTGGCTGG ATTGTGTTAG TCATCACGAC 
CAGCTTTTCA GAGATGAGTA TGCGCTGTAT AGACCACTGC AGAGTACAGA ATTTTCTGAG 
ACGCCTTCTC CCGACAGTGA CTCAGTGAAC TCCGTGGAAG GACACTCTGA GCCATCCTGG 
TTTAAAGACA TAAAGTTTGA TGACAGTGAC ACAGAACAGA TAGCTGAAGA AGGTGACGAT 
AATTTGGCTA ATTCTGCCAG TCCTAGCAAG CGCACATCAG TCAGCAGTTT CCAGTCCACA 
GTGGACAGTG ACTCAGCCGC TTCTATCAGC CTGAACGTGG AGCTGGACAA CGTGAACTTC 
CATATCAAGA AGCCCTCCAA GTACCCACAT GTGCCCCCTC ACCCTGCTGA CCAAAAAGAG 
TATTTGATTT CTGACACTGG AGGACAACAG CTCTCAATAA GTGACGCTTT CATCAAAGAA 
TCCTTATTTA ATCGCCGAGT AGAGGAAAAA TCCAAAGAGC TGCCTTTCAC ACCTTTGGGC 
TGGCATCATA ACAACCTGGA GCTCCTGAGG GAGGAGAATG GGGAGAAACA AGCCATGGAG 
AGGTTGCTTT CAGCTAATCA TAACCACATG ATGGCACTAC TCCAGCAGTT GCTCCATAGT 
GACTCACTGT CATCATCTTG GAGGGACATC ATCGTGTCAT TGGTCTGCCA GGTTGTTCAG 
ACAGTCCGAC CTGATGTCAA GAACCAGGAT GATGACATGG ATATCCGTCA GTTTGTCCAC 
ATCAAAAAAA TCCCAGGTGG AAAGAAGTTT GATTCTGTGG TTGTCAATGG CTTTGTTTGT 
ACCAAGAACA TTGCACATAA AAAGATGAGT TCTTGTATTA AAAACCCCAA AATTCTTCTG 
TTGAAGTGTT CCATTGAGTA TCTCTACAGA GAAGAAACTA AGTTTACTTG CATTGATCCT 
ATTGTGCTTC AGGAAAGGGA ATTCTTGAAG AATTATGTCC AGCGAATAGT TGATGTTCGA 
CCCACCTTGG TTCTTGTTGA GAAAACAGTG TCTCGGATTG CCCAGGACAT GTTATTGGAA 
CATGGCATTA CTTTGGTCAT TAATGTAAAG TCACAAGTTT TGGAACGAAT CAGTCGAATG 
ACCCAAGGTG ATTTAGTGAT GTCAATGGAC CAGCTGCTTA CGAAACCACA CCTGGGCACT 
TGTCACAAAT TTTATATGCA GATATTTCAG TTGCCTAATG AACAAACCAA GACACTGATG 
TTTTTTGAAG GTTGTCCACA GCACCTAGGC TGTACAATCA AGCTAAGAGG AGGCTCTGAT 
TATGAGCTGG CTCGAGTTAA GGAGATCCTA ATATTTATGA TCTGTGTTGC TTATCATTCT 
CAACTAGAAA TATCCTTTCT CATGGATGAA TTTGCTATGC CTCCCACATT AATGCAAAAC 
CCTTCATTCC ATTCCCTGAT TGAGGGACGA GGGCATGAGG GGGCTGTCCA AGAGCAGTAC 
GGTGGAGGTT CCATCCCCTG GGATCCTGAC ATCCCTCCTG AGTCTCTGCC CTGTGATGAT 
AGCAGTTTGC TGGAATTGAG GATTGTGTTT GAGAAGGGTG AGCAGGAAAA TAAAAATCTT 
CCGCAGGCTG TTGCCTCTGT GAAGCATCAA GAACATAGCA CAACAGCTTG CCCGGCGGGT 
CTCCCTTGTG CTTTCTTTGC ACCTGTACCG GAATCATTGT TGCCACTCCC TGTGGATGAC 
CAACAAGATG CTTTAGGCAG CGAGCAGCCA GAGACTTTGC AGCAAACAGT TGTGCTGCAG 
GATCCCAAAA GCCAGATAAG AGCCTTTAGA GACCCTCTAC AGGATGACAC TGGATTATAT 
GTTACTGAGG AAGTCACCTC CTCTGAAGAT AAACGAAAGA CTTATTCTTT GGCCTTTAAG 
CAGGAATTAA AAGATGTGAT CCTCTGTATC TCCCCAGTAA TCACATTCCG AGAACCCTTT 
CTTTTAACTG AAAAGGGGAT GAGATGCTCT ACCCGAGATT ATTTTGCAGA GCAGGTTTAC 
TGGTCTCCTC TCCTCAATAA AGAATTCAAA GAAATGGAGA ACAGGAGGAA GAAACAGCTG 
CTCAGGGATC TCTCTGGACT TCAGGGCATG AATGGAAGTA TTCAGGCCAA GTCTATTCAA 
GTCTTACCCT CACATGAGCT AGTGAGCACT AGAATTGCTG AGCATCTGGG CGATAGCCAG 
AGCTTGGGTA GAATGCTGGC CGATTATCGA GCCAGAGGAG GAAGAATTCA GCCCAAAAAT 
TCAGACCCTT TTGCTCATTC AAAGGATGCA TCAAGTACTT CAAGTGGCCA ATCAGGAAGC 
AAAAATGAGG GTGATGAAGA GAGAGGGCTT ATTCTGAGTG ATGCTGTGTG GTCAACAAAG 
GTGGACTGTC TGAATCCCAT TAATCACCAG AGACTTTGTG TGCTCTTCAG CAGCTCTTCT 
GCCCAGTCCA GCAATGCTCC TAGTGCCTGT GTCAGTCCTT GGATTGTAAC AATGGAATTT 
TATGGAAAGA ATGATCTTAC ATTAGGAATA TTTTTAGAGA GATACTGTTT CAGGCCTTCT 
TATCAGTGTC CAAGCATGTT CTGTGATACC CCCATGGTAC ATCATATTCG GCGCTTTGTT 
CATGGCCAAG GCTGTGTGCA GATAATCCTG AAGGAGTTGG ATTCTCCAGT ACCTGGATAT 
CAGCATACAA TTCTTACATA TTCCTGGTGT AGAATCTGCA AACAGGTAAC ACCAGTTGTT 
GCTCTTTCCA ATGAGTCCTG GTCTATGTCA TTTGCAAAAT ACCTTGAACT TAGGTTTTAT 
GGGCACCAGT ATACTCGCAG AGCCAACGCT GAGCCCTGTG GTCACTCCAT CCATCATGAT 
TATCACCAGT ATTTCTCCTA TAACCAGATG GTGGCGTCTT TCAGTTATTC TCCCATTCGG 
CTTCTTGAAG TATGTGTTCC ACTCCCCAAA ATATTCATTA AGCGTCAGGC CCCATTAAAA 
GTGTCCCTTC TTCAGGATCT GAAGGACTTC TTTCAAAAAG TTTCACAGGT ATATGTTGCC 
ATTGATGAAA GACTTGCATC TTTGAAAACT GATACATTTA GTAAAACAAG AGAGGAAAAA 
ATGGAAGATA TTTTTGCACA GAAAGAGATG GAAGAAGGTG AGTTCAAGAA CTGGATTGAG 
AAGATGCAAG CAAGGCTCAT GTCTTCCTCT GTAGATACCC CTCAGCAACT GCAGTCGGTC 
TTTGAGTCAC TCATTGCCAA GAAACAAAGT CTCTGTGAAG TGCTGCAAGC TTGGAATAAC 
AGGTTGCAGG ACCTTTTCCA ACAGGAAAAG GGTAGAAAGA GACCTTCAGT TCCTCCAAGT 
CCTGGAAGAC TGAGACAAGG GGAAGAAAGC AAGATAAGTG CGATGGATGC ATCTCCACGG 
AATATTTCTC CAGGACTTCA GAATGGAGAA AAAGAGGATC GCTTCTTAAC AACTTTGTCC 
AGCCAGAGCT CCACCAGTTC TACTCATCTC CAATTGCCTA CGCCACCTGA AGTCATGTCT 
GAACAGTCAG TGGGAGGGCC CCCTGAGCTA GATACAGCCA GCAGTTCCGA AGATGTGTTT 
GATGGGCATT TGCTGGGATC CACAGACAGC CAAGTGAAGG AAAAGTCAAC CATGAAAGCC 
ATCTTTGCAA ATTTGCTTCC AGGAAATAGC TATAATCCTA TTCCATTTCC TTTTGATCCA 
GATAAACACT ACTTAATGTA TGAACATGAA CGAGTGCCCA TTGCAGTCTG CGAGAAGGAA 
CCCAGCTCCA TCATTGCTTT TGCTCTCAGT TGTAAAGAAT ACCGAAATGC CTTAGAGGAA 
TTGTCTAAAG CGACTCAGTG GAACAGTGCC GAAGAAGGGC TTCCAACAAA TAGTACTTCA 
GATAGCAGAC CAAAGAGTAG CAGCCCTATC AGATTACCTG AAATGAGTGG AGGACAGACA 
AATCGTACAA CAGAAACAGA ACCACAACCA ACCAAAAAGG CTTCTGGAAT GTTGTCCTTC 
TTCAGAGGGA CAGCAGGGAA AAGCCCCGAT CTCTCTTCCC AGAAGAGAGA GACCTTACGT 
GGAGCAGATA GTGCTTACTA CCAGGTTGGG CAGACGGGCA AGGAGGGGAC CGAGAATCAA 
GGCGTTGAGC CTCAAGATGA AGTAGATGGA GGAGATACAC AAAAGAAGCA ACTCATAAAT 
CCTCATGTGG AACTTCAATT TTCAGATGCT AATGCCAAGT TTTACTGTCG GCTCTACTAT 
GCGGGAGAGT TTCATAAGAT GCGTGAAGTG ATTCTGGACA GCAGTGAAGA AGATTTCATT 
CGTTCCCTCT CCCACTCATC ACCCTGGCAG GCCCGGGGAG GCAAATCAGG AGCTGCCTTC 
TATGCAACTG AGGATGATAG ATTTATTTTG AAGCAAATGC CTCGTCTGGA AGTCCAGTCC 
TTCCTCGACT TTGCACCACA TTACTTCAAT TATATTACAA ATGCTGTTCA ACAAAAGAGG 
CCCACGGCGT TGGCCAAAAT TCTTGGAGTT TACAGAATTG GTTATAAGAA CTCTCAGAAC 
AACACTGAGA AGAAGTTAGA TCTCCTTGTC ATGGAAAATC TTTTCTACGG GAGAAAGATG 
GCACAGGTTT TTGATTTGAA GGGCTCTCTT AGGAATCGGA ATGTAAAAAC TGACACTGGA 
AAAGAGAGTT GTGATGTGGT CCTGCTAGAT GAAAATCTCC TAAAGATGGT TCGAGACAAC 
CCTCTATATA TTCGTTCTCA TTCCAAAGCT GTGCTGAGAA CCTCGATCCA TAGTGACTCC 
CATTTCCTTT CTAGCCACCT CATTATAGAT TATTCTTTGC TGGTTGGGCG AGATGATACT 
AGCAATGAGC TAGTAGTTGG AATTATAGAT TATATTCGAA CATTTACATG GGACAAAAAG 
CTTGAGATGG TTGTGAAATC AACAGGAATT TTAGGTGGAC AAGGTAAAAT GCCAACAGTG 
GTGTCTCCGG AGTTGTACAG GACTAGGTTT TGTGAGGCAA TGGACAAGTA TTTCCTAATG 
GTACCAGACC ACTGGACAGG CTTGGGTCTG AATTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PIKFYVE ( XM_011510788.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011509090.1 Homo sapiens phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X13, mRNA.

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CloneID	OHu77670
Clone ID Related Accession (Same CDS sequence)	XM_011510788.1
Accession Version	XM_011510788.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6006bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	1-phosphatidylinositol 3-phosphate 5-kinase isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG ATGATAAGAC GTCCCCAACA CTGGACTCTG CTAATGATTT GCCTCGATCT 
CCTACTAGTC CTTCTCATCT CACACACTTT AAACCTTTGA CTCCTGATCA AGATGAGCCC 
CCTTTTAAAT CAGCTTATAG TTCTTTTGTA AATCTCTTTC GTTTTAACAA AGAGAGAGCA 
GAAGGAGGCC AGGGAGAACA GCAGCCTTTG AGTGGAAGTT GGACCAGCCC TCAGCTCCCT 
TCGAGGACAC AGTCTGTTAG GTCACCCACA CCTTATAAAA AGCAGCTTAA TGAGGAACTC 
CAGCGGCGCT CTTCAGCATT AGGAGACCTC CGAGCTTGCA CATATTGTAG AAAAATAGCC 
TTAAGTTATG CTCATTCCAC AGACAGTAAT TCTATTGGGG AAGACTTGAA TGCTCTTTCA 
GATTCTGCTT GCTCTGTGTC TGTGCTTGAT CCAAGTGAAC CCCGAACACC TGTTGGGAGT 
AGGAAAGCCA GCCGTAACAT ATTTTTAGAG GATGATTTGG CCTGGCAAAG TTTGATTCAT 
CCAGATTCCT CAAATACTCC TCTTTCAACA AGACTTGTAT CTGTGCAAGA GGATGCTGGG 
AAATCTCCTG CTCGAAATAG ATCAGCCAGC ATTACTAACC TGTCACTGGA TAGATCTGGT 
TCTCCTATGG TACCTTCATA TGAGACATCT GTCAGTCCCC AGGCTAACCG AACATATGTT 
AGGACAGAGA CCACTGAGGA TGAACGCAAA ATTCTTCTGG ACAGTGTGCA GTTAAAAGAC 
CTGTGGAAAA AAATCTGCCA TCACAGCAGT GGAATGGAGT TTCAGGATCA CCGCTACTGG 
TTGAGAACGC ATCCCAACTG CATTGTAGGA AAGGAATTAG TCAACTGGCT AATCCGAAAT 
GGGCATATTG CCACAAGGGC ACAAGCTATA GCAATTGGAC AAGCAATGGT TGATGGACGT 
TGGCTGGATT GTGTTAGTCA TCACGACCAG CTTTTCAGAG ATGAGTATGC GCTGTATAGA 
CCACTGCAGA GTACAGAATT TTCTGAGACG CCTTCTCCCG ACAGTGACTC AGTGAACTCC 
GTGGAAGGAC ACTCTGAGCC ATCCTGGTTT AAAGACATAA AGTTTGATGA CAGTGACACA 
GAACAGATAG CTGAAGAAGG TGACGATAAT TTGGCTAATT CTGCCAGTCC TAGCAAGCGC 
ACATCAGTCA GCAGTTTCCA GTCCACAGTG GACAGTGACT CAGCCGCTTC TATCAGCCTG 
AACGTGGAGC TGGACAACGT GAACTTCCAT ATCAAGAAGC CCTCCAAGTA CCCACATGTG 
CCCCCTCACC CTGCTGACCA AAAAGAGTAT TTGATTTCTG ACACTGGAGG ACAACAGCTC 
TCAATAAGTG ACGCTTTCAT CAAAGAATCC TTATTTAATC GCCGAGTAGA GGAAAAATCC 
AAAGAGCTGC CTTTCACACC TTTGGGCTGG CATCATAACA ACCTGGAGCT CCTGAGGGAG 
GAGAATGGGG AGAAACAAGC CATGGAGAGG TTGCTTTCAG CTAATCATAA CCACATGATG 
GCACTACTCC AGCAGTTGCT CCATAGTGAC TCACTGTCAT CATCTTGGAG GGACATCATC 
GTGTCATTGG TCTGCCAGGT TGTTCAGACA GTCCGACCTG ATGTCAAGAA CCAGGATGAT 
GACATGGATA TCCGTCAGTT TGTCCACATC AAAAAAATCC CAGGTGGAAA GAAGTTTGAT 
TCTGTGGTTG TCAATGGCTT TGTTTGTACC AAGAACATTG CACATAAAAA GATGAGTTCT 
TGTATTAAAA ACCCCAAAAT TCTTCTGTTG AAGTGTTCCA TTGAGTATCT CTACAGAGAA 
GAAACTAAGT TTACTTGCAT TGATCCTATT GTGCTTCAGG AAAGGGAATT CTTGAAGAAT 
TATGTCCAGC GAATAGTTGA TGTTCGACCC ACCTTGGTTC TTGTTGAGAA AACAGTGTCT 
CGGATTGCCC AGGACATGTT ATTGGAACAT GGCATTACTT TGGTCATTAA TGTAAAGTCA 
CAAGTTTTGG AACGAATCAG TCGAATGACC CAAGGTGATT TAGTGATGTC AATGGACCAG 
CTGCTTACGA AACCACACCT GGGCACTTGT CACAAATTTT ATATGCAGAT ATTTCAGTTG 
CCTAATGAAC AAACCAAGAC ACTGATGTTT TTTGAAGGTT GTCCACAGCA CCTAGGCTGT 
ACAATCAAGC TAAGAGGAGG CTCTGATTAT GAGCTGGCTC GAGTTAAGGA GATCCTAATA 
TTTATGATCT GTGTTGCTTA TCATTCTCAA CTAGAAATAT CCTTTCTCAT GGATGAATTT 
GCTATGCCTC CCACATTAAT GCAAAACCCT TCATTCCATT CCCTGATTGA GGGACGAGGG 
CATGAGGGGG CTGTCCAAGA GCAGTACGGT GGAGGTTCCA TCCCCTGGGA TCCTGACATC 
CCTCCTGAGT CTCTGCCCTG TGATGATAGC AGTTTGCTGG AATTGAGGAT TGTGTTTGAG 
AAGGGTGAGC AGGAAAATAA AAATCTTCCG CAGGCTGTTG CCTCTGTGAA GCATCAAGAA 
CATAGCACAA CAGCTTGCCC GGCGGGTCTC CCTTGTGCTT TCTTTGCACC TGTACCGGAA 
TCATTGTTGC CACTCCCTGT GGATGACCAA CAAGATGCTT TAGGCAGCGA GCAGCCAGAG 
ACTTTGCAGC AAACAGTTGT GCTGCAGGAT CCCAAAAGCC AGATAAGAGC CTTTAGAGAC 
CCTCTACAGG ATGACACTGG ATTATATGTT ACTGAGGAAG TCACCTCCTC TGAAGATAAA 
CGAAAGACTT ATTCTTTGGC CTTTAAGCAG GAATTAAAAG ATGTGATCCT CTGTATCTCC 
CCAGTAATCA CATTCCGAGA ACCCTTTCTT TTAACTGAAA AGGGGATGAG ATGCTCTACC 
CGAGATTATT TTGCAGAGCA GGTTTACTGG TCTCCTCTCC TCAATAAAGA ATTCAAAGAA 
ATGGAGAACA GGAGGAAGAA ACAGCTGCTC AGGGATCTCT CTGGACTTCA GGGCATGAAT 
GGAAGTATTC AGGCCAAGTC TATTCAAGTC TTACCCTCAC ATGAGCTAGT GAGCACTAGA 
ATTGCTGAGC ATCTGGGCGA TAGCCAGAGC TTGGGTAGAA TGCTGGCCGA TTATCGAGCC 
AGAGGAGGAA GAATTCAGCC CAAAAATTCA GACCCTTTTG CTCATTCAAA GGATGCATCA 
AGTACTTCAA GTGGCCAATC AGGAAGCAAA AATGAGGGTG ATGAAGAGAG AGGGCTTATT 
CTGAGTGATG CTGTGTGGTC AACAAAGGTG GACTGTCTGA ATCCCATTAA TCACCAGAGA 
CTTTGTGTGC TCTTCAGCAG CTCTTCTGCC CAGTCCAGCA ATGCTCCTAG TGCCTGTGTC 
AGTCCTTGGA TTGTAACAAT GGAATTTTAT GGAAAGAATG ATCTTACATT AGGAATATTT 
TTAGAGAGAT ACTGTTTCAG GCCTTCTTAT CAGTGTCCAA GCATGTTCTG TGATACCCCC 
ATGGTACATC ATATTCGGCG CTTTGTTCAT GGCCAAGGCT GTGTGCAGAT AATCCTGAAG 
GAGTTGGATT CTCCAGTACC TGGATATCAG CATACAATTC TTACATATTC CTGGTGTAGA 
ATCTGCAAAC AGGTAACACC AGTTGTTGCT CTTTCCAATG AGTCCTGGTC TATGTCATTT 
GCAAAATACC TTGAACTTAG GTTTTATGGG CACCAGTATA CTCGCAGAGC CAACGCTGAG 
CCCTGTGGTC ACTCCATCCA TCATGATTAT CACCAGTATT TCTCCTATAA CCAGATGGTG 
GCGTCTTTCA GTTATTCTCC CATTCGGCTT CTTGAAGTAT GTGTTCCACT CCCCAAAATA 
TTCATTAAGC GTCAGGCCCC ATTAAAAGTG TCCCTTCTTC AGGATCTGAA GGACTTCTTT 
CAAAAAGTTT CACAGGTATA TGTTGCCATT GATGAAAGAC TTGCATCTTT GAAAACTGAT 
ACATTTAGTA AAACAAGAGA GGAAAAAATG GAAGATATTT TTGCACAGAA AGAGATGGAA 
GAAGGTGAGT TCAAGAACTG GATTGAGAAG ATGCAAGCAA GGCTCATGTC TTCCTCTGTA 
GATACCCCTC AGCAACTGCA GTCGGTCTTT GAGTCACTCA TTGCCAAGAA ACAAAGTCTC 
TGTGAAGTGC TGCAAGCTTG GAATAACAGG TTGCAGGACC TTTTCCAACA GGAAAAGGGT 
AGAAAGAGAC CTTCAGTTCC TCCAAGTCCT GGAAGACTGA GACAAGGGGA AGAAAGCAAG 
ATAAGTGCGA TGGATGCATC TCCACGGAAT ATTTCTCCAG GACTTCAGAA TGGAGAAAAA 
GAGGATCGCT TCTTAACAAC TTTGTCCAGC CAGAGCTCCA CCAGTTCTAC TCATCTCCAA 
TTGCCTACGC CACCTGAAGT CATGTCTGAA CAGTCAGTGG GAGGGCCCCC TGAGCTAGAT 
ACAGCCAGCA GTTCCGAAGA TGTGTTTGAT GGGCATTTGC TGGGATCCAC AGACAGCCAA 
GTGAAGGAAA AGTCAACCAT GAAAGCCATC TTTGCAAATT TGCTTCCAGG AAATAGCTAT 
AATCCTATTC CATTTCCTTT TGATCCAGAT AAACACTACT TAATGTATGA ACATGAACGA 
GTGCCCATTG CAGTCTGCGA GAAGGAACCC AGCTCCATCA TTGCTTTTGC TCTCAGTTGT 
AAAGAATACC GAAATGCCTT AGAGGAATTG TCTAAAGCGA CTCAGTGGAA CAGTGCCGAA 
GAAGGGCTTC CAACAAATAG TACTTCAGAT AGCAGACCAA AGAGTAGCAG CCCTATCAGA 
TTACCTGAAA TGAGTGGAGG ACAGACAAAT CGTACAACAG AAACAGAACC ACAACCAACC 
AAAAAGGCTT CTGGAATGTT GTCCTTCTTC AGAGGGACAG CAGGGAAAAG CCCCGATCTC 
TCTTCCCAGA AGAGAGAGAC CTTACGTGGA GCAGATAGTG CTTACTACCA GGTTGGGCAG 
ACGGGCAAGG AGGGGACCGA GAATCAAGGC GTTGAGCCTC AAGATGAAGT AGATGGAGGA 
GATACACAAA AGAAGCAACT CATAAATCCT CATGTGGAAC TTCAATTTTC AGATGCTAAT 
GCCAAGTTTT ACTGTCGGCT CTACTATGCG GGAGAGTTTC ATAAGATGCG TGAAGTGATT 
CTGGACAGCA GTGAAGAAGA TTTCATTCGT TCCCTCTCCC ACTCATCACC CTGGCAGGCC 
CGGGGAGGCA AATCAGGAGC TGCCTTCTAT GCAACTGAGG ATGATAGATT TATTTTGAAG 
CAAATGCCTC GTCTGGAAGT CCAGTCCTTC CTCGACTTTG CACCACATTA CTTCAATTAT 
ATTACAAATG CTGTTCAACA AAAGAGGCCC ACGGCGTTGG CCAAAATTCT TGGAGTTTAC 
AGAATTGGTT ATAAGAACTC TCAGAACAAC ACTGAGAAGA AGTTAGATCT CCTTGTCATG 
GAAAATCTTT TCTACGGGAG AAAGATGGCA CAGGTTTTTG ATTTGAAGGG CTCTCTTAGG 
AATCGGAATG TAAAAACTGA CACTGGAAAA GAGAGTTGTG ATGTGGTCCT GCTAGATGAA 
AATCTCCTAA AGATGGTTCG AGACAACCCT CTATATATTC GTTCTCATTC CAAAGCTGTG 
CTGAGAACCT CGATCCATAG TGACTCCCAT TTCCTTTCTA GCCACCTCAT TATAGATTAT 
TCTTTGCTGG TTGGGCGAGA TGATACTAGC AATGAGCTAG TAGTTGGAAT TATAGATTAT 
ATTCGAACAT TTACATGGGA CAAAAAGCTT GAGATGGTTG TGAAATCAAC AGGAATTTTA 
GGTGGACAAG GTAAAATGCC AACAGTGGTG TCTCCGGAGT TGTACAGGAC TAGGTTTTGT 
GAGGCAATGG ACAAGTATTT CCTAATGGTA CCAGACCACT GGACAGGCTT GGGTCTGAAT 
TGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011509090.1
CDS	159..6164
Translation	MATDDKTSPTLDSANDLPRSPTSPSHLTHFKPLTPDQDEPPFKSAYSSFVNLFRFNKERAEGGQGEQQPLSGSWTSPQLPSRTQSVRSPTPYKKQLNEELQRRSSALGDLRACTYCRKIALSYAHSTDSNSIGEDLNALSDSACSVSVLDPSEPRTPVGSRKASRNIFLEDDLAWQSLIHPDSSNTPLSTRLVSVQEDAGKSPARNRSASITNLSLDRSGSPMVPSYETSVSPQANRTYVRTETTEDERKILLDSVQLKDLWKKICHHSSGMEFQDHRYWLRTHPNCIVGKELVNWLIRNGHIATRAQAIAIGQAMVDGRWLDCVSHHDQLFRDEYALYRPLQSTEFSETPSPDSDSVNSVEGHSEPSWFKDIKFDDSDTEQIAEEGDDNLANSASPSKRTSVSSFQSTVDSDSAASISLNVELDNVNFHIKKPSKYPHVPPHPADQKEYLISDTGGQQLSISDAFIKESLFNRRVEEKSKELPFTPLGWHHNNLELLREENGEKQAMERLLSANHNHMMALLQQLLHSDSLSSSWRDIIVSLVCQVVQTVRPDVKNQDDDMDIRQFVHIKKIPGGKKFDSVVVNGFVCTKNIAHKKMSSCIKNPKILLLKCSIEYLYREETKFTCIDPIVLQEREFLKNYVQRIVDVRPTLVLVEKTVSRIAQDMLLEHGITLVINVKSQVLERISRMTQGDLVMSMDQLLTKPHLGTCHKFYMQIFQLPNEQTKTLMFFEGCPQHLGCTIKLRGGSDYELARVKEILIFMICVAYHSQLEISFLMDEFAMPPTLMQNPSFHSLIEGRGHEGAVQEQYGGGSIPWDPDIPPESLPCDDSSLLELRIVFEKGEQENKNLPQAVASVKHQEHSTTACPAGLPCAFFAPVPESLLPLPVDDQQDALGSEQPETLQQTVVLQDPKSQIRAFRDPLQDDTGLYVTEEVTSSEDKRKTYSLAFKQELKDVILCISPVITFREPFLLTEKGMRCSTRDYFAEQVYWSPLLNKEFKEMENRRKKQLLRDLSGLQGMNGSIQAKSIQVLPSHELVSTRIAEHLGDSQSLGRMLADYRARGGRIQPKNSDPFAHSKDASSTSSGQSGSKNEGDEERGLILSDAVWSTKVDCLNPINHQRLCVLFSSSSAQSSNAPSACVSPWIVTMEFYGKNDLTLGIFLERYCFRPSYQCPSMFCDTPMVHHIRRFVHGQGCVQIILKELDSPVPGYQHTILTYSWCRICKQVTPVVALSNESWSMSFAKYLELRFYGHQYTRRANAEPCGHSIHHDYHQYFSYNQMVASFSYSPIRLLEVCVPLPKIFIKRQAPLKVSLLQDLKDFFQKVSQVYVAIDERLASLKTDTFSKTREEKMEDIFAQKEMEEGEFKNWIEKMQARLMSSSVDTPQQLQSVFESLIAKKQSLCEVLQAWNNRLQDLFQQEKGRKRPSVPPSPGRLRQGEESKISAMDASPRNISPGLQNGEKEDRFLTTLSSQSSTSSTHLQLPTPPEVMSEQSVGGPPELDTASSSEDVFDGHLLGSTDSQVKEKSTMKAIFANLLPGNSYNPIPFPFDPDKHYLMYEHERVPIAVCEKEPSSIIAFALSCKEYRNALEELSKATQWNSAEEGLPTNSTSDSRPKSSSPIRLPEMSGGQTNRTTETEPQPTKKASGMLSFFRGTAGKSPDLSSQKRETLRGADSAYYQVGQTGKEGTENQGVEPQDEVDGGDTQKKQLINPHVELQFSDANAKFYCRLYYAGEFHKMREVILDSSEEDFIRSLSHSSPWQARGGKSGAAFYATEDDRFILKQMPRLEVQSFLDFAPHYFNYITNAVQQKRPTALAKILGVYRIGYKNSQNNTEKKLDLLVMENLFYGRKMAQVFDLKGSLRNRNVKTDTGKESCDVVLLDENLLKMVRDNPLYIRSHSKAVLRTSIHSDSHFLSSHLIIDYSLLVGRDDTSNELVVGIIDYIRTFTWDKKLEMVVKSTGILGGQGKMPTVVSPELYRTRFCEAMDKYFLMVPDHWTGLGLNC

Target ORF information:

RefSeq Version	XM_011510788.1
Organism	Homo sapiens(human)
Definition	Homo sapiens phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011510788.1

ORF Insert Sequence:

ATGGCCACAG ATGATAAGAC GTCCCCAACA CTGGACTCTG CTAATGATTT GCCTCGATCT 
CCTACTAGTC CTTCTCATCT CACACACTTT AAACCTTTGA CTCCTGATCA AGATGAGCCC 
CCTTTTAAAT CAGCTTATAG TTCTTTTGTA AATCTCTTTC GTTTTAACAA AGAGAGAGCA 
GAAGGAGGCC AGGGAGAACA GCAGCCTTTG AGTGGAAGTT GGACCAGCCC TCAGCTCCCT 
TCGAGGACAC AGTCTGTTAG GTCACCCACA CCTTATAAAA AGCAGCTTAA TGAGGAACTC 
CAGCGGCGCT CTTCAGCATT AGGAGACCTC CGAGCTTGCA CATATTGTAG AAAAATAGCC 
TTAAGTTATG CTCATTCCAC AGACAGTAAT TCTATTGGGG AAGACTTGAA TGCTCTTTCA 
GATTCTGCTT GCTCTGTGTC TGTGCTTGAT CCAAGTGAAC CCCGAACACC TGTTGGGAGT 
AGGAAAGCCA GCCGTAACAT ATTTTTAGAG GATGATTTGG CCTGGCAAAG TTTGATTCAT 
CCAGATTCCT CAAATACTCC TCTTTCAACA AGACTTGTAT CTGTGCAAGA GGATGCTGGG 
AAATCTCCTG CTCGAAATAG ATCAGCCAGC ATTACTAACC TGTCACTGGA TAGATCTGGT 
TCTCCTATGG TACCTTCATA TGAGACATCT GTCAGTCCCC AGGCTAACCG AACATATGTT 
AGGACAGAGA CCACTGAGGA TGAACGCAAA ATTCTTCTGG ACAGTGTGCA GTTAAAAGAC 
CTGTGGAAAA AAATCTGCCA TCACAGCAGT GGAATGGAGT TTCAGGATCA CCGCTACTGG 
TTGAGAACGC ATCCCAACTG CATTGTAGGA AAGGAATTAG TCAACTGGCT AATCCGAAAT 
GGGCATATTG CCACAAGGGC ACAAGCTATA GCAATTGGAC AAGCAATGGT TGATGGACGT 
TGGCTGGATT GTGTTAGTCA TCACGACCAG CTTTTCAGAG ATGAGTATGC GCTGTATAGA 
CCACTGCAGA GTACAGAATT TTCTGAGACG CCTTCTCCCG ACAGTGACTC AGTGAACTCC 
GTGGAAGGAC ACTCTGAGCC ATCCTGGTTT AAAGACATAA AGTTTGATGA CAGTGACACA 
GAACAGATAG CTGAAGAAGG TGACGATAAT TTGGCTAATT CTGCCAGTCC TAGCAAGCGC 
ACATCAGTCA GCAGTTTCCA GTCCACAGTG GACAGTGACT CAGCCGCTTC TATCAGCCTG 
AACGTGGAGC TGGACAACGT GAACTTCCAT ATCAAGAAGC CCTCCAAGTA CCCACATGTG 
CCCCCTCACC CTGCTGACCA AAAAGAGTAT TTGATTTCTG ACACTGGAGG ACAACAGCTC 
TCAATAAGTG ACGCTTTCAT CAAAGAATCC TTATTTAATC GCCGAGTAGA GGAAAAATCC 
AAAGAGCTGC CTTTCACACC TTTGGGCTGG CATCATAACA ACCTGGAGCT CCTGAGGGAG 
GAGAATGGGG AGAAACAAGC CATGGAGAGG TTGCTTTCAG CTAATCATAA CCACATGATG 
GCACTACTCC AGCAGTTGCT CCATAGTGAC TCACTGTCAT CATCTTGGAG GGACATCATC 
GTGTCATTGG TCTGCCAGGT TGTTCAGACA GTCCGACCTG ATGTCAAGAA CCAGGATGAT 
GACATGGATA TCCGTCAGTT TGTCCACATC AAAAAAATCC CAGGTGGAAA GAAGTTTGAT 
TCTGTGGTTG TCAATGGCTT TGTTTGTACC AAGAACATTG CACATAAAAA GATGAGTTCT 
TGTATTAAAA ACCCCAAAAT TCTTCTGTTG AAGTGTTCCA TTGAGTATCT CTACAGAGAA 
GAAACTAAGT TTACTTGCAT TGATCCTATT GTGCTTCAGG AAAGGGAATT CTTGAAGAAT 
TATGTCCAGC GAATAGTTGA TGTTCGACCC ACCTTGGTTC TTGTTGAGAA AACAGTGTCT 
CGGATTGCCC AGGACATGTT ATTGGAACAT GGCATTACTT TGGTCATTAA TGTAAAGTCA 
CAAGTTTTGG AACGAATCAG TCGAATGACC CAAGGTGATT TAGTGATGTC AATGGACCAG 
CTGCTTACGA AACCACACCT GGGCACTTGT CACAAATTTT ATATGCAGAT ATTTCAGTTG 
CCTAATGAAC AAACCAAGAC ACTGATGTTT TTTGAAGGTT GTCCACAGCA CCTAGGCTGT 
ACAATCAAGC TAAGAGGAGG CTCTGATTAT GAGCTGGCTC GAGTTAAGGA GATCCTAATA 
TTTATGATCT GTGTTGCTTA TCATTCTCAA CTAGAAATAT CCTTTCTCAT GGATGAATTT 
GCTATGCCTC CCACATTAAT GCAAAACCCT TCATTCCATT CCCTGATTGA GGGACGAGGG 
CATGAGGGGG CTGTCCAAGA GCAGTACGGT GGAGGTTCCA TCCCCTGGGA TCCTGACATC 
CCTCCTGAGT CTCTGCCCTG TGATGATAGC AGTTTGCTGG AATTGAGGAT TGTGTTTGAG 
AAGGGTGAGC AGGAAAATAA AAATCTTCCG CAGGCTGTTG CCTCTGTGAA GCATCAAGAA 
CATAGCACAA CAGCTTGCCC GGCGGGTCTC CCTTGTGCTT TCTTTGCACC TGTACCGGAA 
TCATTGTTGC CACTCCCTGT GGATGACCAA CAAGATGCTT TAGGCAGCGA GCAGCCAGAG 
ACTTTGCAGC AAACAGTTGT GCTGCAGGAT CCCAAAAGCC AGATAAGAGC CTTTAGAGAC 
CCTCTACAGG ATGACACTGG ATTATATGTT ACTGAGGAAG TCACCTCCTC TGAAGATAAA 
CGAAAGACTT ATTCTTTGGC CTTTAAGCAG GAATTAAAAG ATGTGATCCT CTGTATCTCC 
CCAGTAATCA CATTCCGAGA ACCCTTTCTT TTAACTGAAA AGGGGATGAG ATGCTCTACC 
CGAGATTATT TTGCAGAGCA GGTTTACTGG TCTCCTCTCC TCAATAAAGA ATTCAAAGAA 
ATGGAGAACA GGAGGAAGAA ACAGCTGCTC AGGGATCTCT CTGGACTTCA GGGCATGAAT 
GGAAGTATTC AGGCCAAGTC TATTCAAGTC TTACCCTCAC ATGAGCTAGT GAGCACTAGA 
ATTGCTGAGC ATCTGGGCGA TAGCCAGAGC TTGGGTAGAA TGCTGGCCGA TTATCGAGCC 
AGAGGAGGAA GAATTCAGCC CAAAAATTCA GACCCTTTTG CTCATTCAAA GGATGCATCA 
AGTACTTCAA GTGGCCAATC AGGAAGCAAA AATGAGGGTG ATGAAGAGAG AGGGCTTATT 
CTGAGTGATG CTGTGTGGTC AACAAAGGTG GACTGTCTGA ATCCCATTAA TCACCAGAGA 
CTTTGTGTGC TCTTCAGCAG CTCTTCTGCC CAGTCCAGCA ATGCTCCTAG TGCCTGTGTC 
AGTCCTTGGA TTGTAACAAT GGAATTTTAT GGAAAGAATG ATCTTACATT AGGAATATTT 
TTAGAGAGAT ACTGTTTCAG GCCTTCTTAT CAGTGTCCAA GCATGTTCTG TGATACCCCC 
ATGGTACATC ATATTCGGCG CTTTGTTCAT GGCCAAGGCT GTGTGCAGAT AATCCTGAAG 
GAGTTGGATT CTCCAGTACC TGGATATCAG CATACAATTC TTACATATTC CTGGTGTAGA 
ATCTGCAAAC AGGTAACACC AGTTGTTGCT CTTTCCAATG AGTCCTGGTC TATGTCATTT 
GCAAAATACC TTGAACTTAG GTTTTATGGG CACCAGTATA CTCGCAGAGC CAACGCTGAG 
CCCTGTGGTC ACTCCATCCA TCATGATTAT CACCAGTATT TCTCCTATAA CCAGATGGTG 
GCGTCTTTCA GTTATTCTCC CATTCGGCTT CTTGAAGTAT GTGTTCCACT CCCCAAAATA 
TTCATTAAGC GTCAGGCCCC ATTAAAAGTG TCCCTTCTTC AGGATCTGAA GGACTTCTTT 
CAAAAAGTTT CACAGGTATA TGTTGCCATT GATGAAAGAC TTGCATCTTT GAAAACTGAT 
ACATTTAGTA AAACAAGAGA GGAAAAAATG GAAGATATTT TTGCACAGAA AGAGATGGAA 
GAAGGTGAGT TCAAGAACTG GATTGAGAAG ATGCAAGCAA GGCTCATGTC TTCCTCTGTA 
GATACCCCTC AGCAACTGCA GTCGGTCTTT GAGTCACTCA TTGCCAAGAA ACAAAGTCTC 
TGTGAAGTGC TGCAAGCTTG GAATAACAGG TTGCAGGACC TTTTCCAACA GGAAAAGGGT 
AGAAAGAGAC CTTCAGTTCC TCCAAGTCCT GGAAGACTGA GACAAGGGGA AGAAAGCAAG 
ATAAGTGCGA TGGATGCATC TCCACGGAAT ATTTCTCCAG GACTTCAGAA TGGAGAAAAA 
GAGGATCGCT TCTTAACAAC TTTGTCCAGC CAGAGCTCCA CCAGTTCTAC TCATCTCCAA 
TTGCCTACGC CACCTGAAGT CATGTCTGAA CAGTCAGTGG GAGGGCCCCC TGAGCTAGAT 
ACAGCCAGCA GTTCCGAAGA TGTGTTTGAT GGGCATTTGC TGGGATCCAC AGACAGCCAA 
GTGAAGGAAA AGTCAACCAT GAAAGCCATC TTTGCAAATT TGCTTCCAGG AAATAGCTAT 
AATCCTATTC CATTTCCTTT TGATCCAGAT AAACACTACT TAATGTATGA ACATGAACGA 
GTGCCCATTG CAGTCTGCGA GAAGGAACCC AGCTCCATCA TTGCTTTTGC TCTCAGTTGT 
AAAGAATACC GAAATGCCTT AGAGGAATTG TCTAAAGCGA CTCAGTGGAA CAGTGCCGAA 
GAAGGGCTTC CAACAAATAG TACTTCAGAT AGCAGACCAA AGAGTAGCAG CCCTATCAGA 
TTACCTGAAA TGAGTGGAGG ACAGACAAAT CGTACAACAG AAACAGAACC ACAACCAACC 
AAAAAGGCTT CTGGAATGTT GTCCTTCTTC AGAGGGACAG CAGGGAAAAG CCCCGATCTC 
TCTTCCCAGA AGAGAGAGAC CTTACGTGGA GCAGATAGTG CTTACTACCA GGTTGGGCAG 
ACGGGCAAGG AGGGGACCGA GAATCAAGGC GTTGAGCCTC AAGATGAAGT AGATGGAGGA 
GATACACAAA AGAAGCAACT CATAAATCCT CATGTGGAAC TTCAATTTTC AGATGCTAAT 
GCCAAGTTTT ACTGTCGGCT CTACTATGCG GGAGAGTTTC ATAAGATGCG TGAAGTGATT 
CTGGACAGCA GTGAAGAAGA TTTCATTCGT TCCCTCTCCC ACTCATCACC CTGGCAGGCC 
CGGGGAGGCA AATCAGGAGC TGCCTTCTAT GCAACTGAGG ATGATAGATT TATTTTGAAG 
CAAATGCCTC GTCTGGAAGT CCAGTCCTTC CTCGACTTTG CACCACATTA CTTCAATTAT 
ATTACAAATG CTGTTCAACA AAAGAGGCCC ACGGCGTTGG CCAAAATTCT TGGAGTTTAC 
AGAATTGGTT ATAAGAACTC TCAGAACAAC ACTGAGAAGA AGTTAGATCT CCTTGTCATG 
GAAAATCTTT TCTACGGGAG AAAGATGGCA CAGGTTTTTG ATTTGAAGGG CTCTCTTAGG 
AATCGGAATG TAAAAACTGA CACTGGAAAA GAGAGTTGTG ATGTGGTCCT GCTAGATGAA 
AATCTCCTAA AGATGGTTCG AGACAACCCT CTATATATTC GTTCTCATTC CAAAGCTGTG 
CTGAGAACCT CGATCCATAG TGACTCCCAT TTCCTTTCTA GCCACCTCAT TATAGATTAT 
TCTTTGCTGG TTGGGCGAGA TGATACTAGC AATGAGCTAG TAGTTGGAAT TATAGATTAT 
ATTCGAACAT TTACATGGGA CAAAAAGCTT GAGATGGTTG TGAAATCAAC AGGAATTTTA 
GGTGGACAAG GTAAAATGCC AACAGTGGTG TCTCCGGAGT TGTACAGGAC TAGGTTTTGT 
GAGGCAATGG ACAAGTATTT CCTAATGGTA CCAGACCACT GGACAGGCTT GGGTCTGAAT 
TGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The Lipid Kinase PIKfyve Coordinates the Neutrophil Immune Response through the Activation of the Rac GTPase.
Journal of immunology (Baltimore, Md. : 1950)199(6)2096-2105(2017 Sep)
Dayam RM,Sun CX,Choy CH,Mancuso G,Glogauer M,Botelho RJ

PIKfyve inhibition increases exosome release and induces secretory autophagy.
Cellular and molecular life sciences : CMLS73(24)4717-4737(2016 Dec)
Hessvik NP,?verbye A,Brech A,Torgersen ML,Jakobsen IS,Sandvig K,Llorente A

PIKfyve Regulates Vacuole Maturation and Nutrient Recovery following Engulfment.
Developmental cell38(5)536-47(2016 09)
Krishna S,Palm W,Lee Y,Yang W,Bandyopadhyay U,Xu H,Florey O,Thompson CB,Overholtzer M

A cell-permeable tool for analysing APP intracellular domain function and manipulation of PIKfyve activity.
Bioscience reports36(2)(2016)
Guscott B,Balklava Z,Safrany ST,Wassmer T

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PIKFYVE cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

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Target ORF information:

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