SPAG17 gene cDNA ORF clone, Homo sapiens(human)

mRNA	Protein	Name
XM_006710427.3	XP_006710490.1	sperm-associated antigen 17 isoform X3
XM_011540936.2	XP_011539238.1	sperm-associated antigen 17 isoform X5
XM_011540939.2	XP_011539241.1	sperm-associated antigen 17 isoform X6
XM_006710428.3	XP_006710491.1	sperm-associated antigen 17 isoform X6
XM_011540941.2	XP_011539243.1	sperm-associated antigen 17 isoform X7
XM_006710426.3	XP_006710489.1	sperm-associated antigen 17 isoform X1
XM_011540935.2	XP_011539237.1	sperm-associated antigen 17 isoform X4
XM_011540942.2	XP_011539244.1	sperm-associated antigen 17 isoform X7
NM_206996.3	NP_996879.1	sperm-associated antigen 17
NM_206996.4	NP_996879.1	sperm-associated antigen 17
XM_011540934.1	XP_011539236.1	sperm-associated antigen 17 isoform X2
XM_011540937.2	XP_011539239.1	sperm-associated antigen 17 isoform X5
XM_006710429.3	XP_006710492.1	sperm-associated antigen 17 isoform X8
XM_006710431.3	XP_006710494.1	sperm-associated antigen 17 isoform X9

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu16428	NM_206996.4 Latest version!	Homo sapiens sperm associated antigen 17 (SPAG17), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$659.50-$923.30 $1319.00
OHu33136	XM_006710431.3 Latest version!	Homo sapiens sperm associated antigen 17 (SPAG17), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OHu33133	XM_006710428.3 Latest version!	Homo sapiens sperm associated antigen 17 (SPAG17), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
OHu33133	XM_011540939.2 Latest version!	Homo sapiens sperm associated antigen 17 (SPAG17), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
OHu33134	XM_006710429.3 Latest version!	Homo sapiens sperm associated antigen 17 (SPAG17), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
OHu77636	XM_011540937.2 Latest version!	Homo sapiens sperm associated antigen 17 (SPAG17), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu77636	XM_011540936.2 Latest version!	Homo sapiens sperm associated antigen 17 (SPAG17), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu77639	XM_011540942.2 Latest version!	Homo sapiens sperm associated antigen 17 (SPAG17), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
OHu77639	XM_011540941.2 Latest version!	Homo sapiens sperm associated antigen 17 (SPAG17), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
OHu33131	XM_006710426.3 Latest version!	Homo sapiens sperm associated antigen 17 (SPAG17), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OHu33132	XM_006710427.3 Latest version!	Homo sapiens sperm associated antigen 17 (SPAG17), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OHu77634	XM_011540934.1 Latest version!	Homo sapiens sperm associated antigen 17 (SPAG17), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OHu77635	XM_011540935.2 Latest version!	Homo sapiens sperm associated antigen 17 (SPAG17), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
View Old Accession Versions >>
OHu16428	NM_206996.3	Homo sapiens sperm associated antigen 17 (SPAG17), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$659.50-$923.30 $1319.00
Hide Old Accession Versions >>

SPAG17 ( NM_206996.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_996879.1 Homo sapiens sperm associated antigen 17 (SPAG17), mRNA.

Price & Availability↑

CloneID	OHu16428
Clone ID Related Accession (Same CDS sequence)	NM_206996.3 , NM_206996.4
Accession Version	NM_206996.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6672bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-09-28
Organism	Homo sapiens(human)
Product	sperm-associated antigen 17
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL513191.4, DB060943.1, AY555274.1 and DB340825.1. This sequence is a reference standard in the RefSeqGene project. On Feb 8, 2017 this sequence version replaced NM_206996.2. ##Evidence-Data-START## Transcript exon combination :: AY555274.1, BX648110.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1966682, SAMEA1968189 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCACCCA AGAAGGAGAA AGGAGGAACT GTGAACACCA GTTCTAAGAT ATGGGAACCC 
TCGCTCATAG CTGCACAGTT CAATCAGAAC GATTGGCAGG CCTCCATTGC TTTTGTGGTT 
GGGAACCAGA TTGAAGATGA TCTTCTCATC CAAGCCCTTA CCGTGGCTGT CCAGGTCCCT 
CAGCGTAAAC TCTTCAGTAT GGTGTCGTGG CAAGACATTC TCCAGCAGAT TAATGAAATA 
AATACACTTG TTGGATCTGC TTCATCTAAA AAGGCAAAAA AACCTGTAGG TGGTAATGCT 
CCTTTATATT ATGAGGTGTT AACGGCAGCA AAAGCAATTA TGGATAGTGG AGAGAAATTA 
ACCTTACCAC TGATAGGGAA ACTCTTGAAA TTTCAACTTC TCCAGATTAA ATTTAAGGAC 
CAACAGCGAC GGGAAAATGA AAAGAAGGTA ATAGAAGACA AACCTAAGTT AGAAAAGGAT 
AAAGGGAAAG CAAAATCTCC CAAGGAGAAA AAGGCTCCAA GTGCCAAGCC TGCCAAAGGA 
AAGGGAAAGG ATCAGCCTGA GGCAAATGCA CCAGTGAAAA AGACCACCCA GTTAAAGCGG 
AGAGGAGAAG ACGACCACAC CAATCGTTAC ATTGACGATG AGCCAGATGA TGGTGCCCAA 
CATTACATTA TAGTTGTGGG CTTTAACAAT CCTCAGCTAT TAGCAATTAT GGCTGAGCTT 
GGCATTCCTA TAACCAGCGT GATTAAAATA TCTTCAGAGA ATTATGAACC TCTGCAGACA 
CACCTGGCAG CAGTTAACCA GCAGCAGGAA GTTCTTCTTC AGTCAGAAGA TCTAGAAGCA 
GAAAAATTGA AGAAAGAAAA TGCCATAAAA GAGCTTAAAA CTTTCTGGAA GTACTTGGAA 
CCAGTCCTGA ATAATGAGAA ACCTGAAACA AATCTCTTTG ATGTTGCTCG ACTTGAGTAC 
ATGGTCAAAG CAGCTGATTT TCCTTCTGAC TGGTCAGATG GTGAGATGAT GCTGAAATTG 
GGCACTGATA TTTTTGAAAA TATTGCCTGC TTGATGTATG ACATCCTGGA TTGGAAAAGG 
CAGCACCAGC ACTATTTGGA AAGCATGCAG CTTATTAATG TTCCACAAGT GGTTAATGAG 
AAACCTGTAT TAGAAGCCAT GCCAACTTCA GAGGCTCCAC AACCTGCTGT ACCAGCTCCT 
GGAAAGAAGA AAGCACAGTA TGAAGAACCG CAAGCTCCAC CACCAGTGAC TTCAGTCATC 
ACAACTGAAG TAGACATGAG ATATTACAAT TATTTGCTGA ATCCAATTCG AGAGGAATTC 
ATTTCTGTGC CCCTGATACT GCATTGTATG CTGGAACAGG TTGTTGCAAC TGAAGAAGAT 
CTCGTCCCAC CCAGTCTGCG GGAGCCATCC CCCAGAGCAG ACGGGCTAGA CCACAGAATC 
GCAGCTCACA TTGTGTCCCT TCTGCCCTCA CTCTGTCTCT CAGAGAGGGA GAAAAAGAAT 
CTTCATGACA TATTTTTATC TGAAGAAGAA AATGAAAGCA AAGCAGTGCC CAAAGGCCCC 
CTCCTACTGA ACTATCATGA TGCACACGCC CACAAGAAGT ACGCACTACA GGACCAAAAG 
AATTTTGATC CAGTTCAAAT TGAGCAGGAG ATGCAGTCCA AGTTGCCACT GTGGGAATTT 
CTTCAATTCC CTCTACCCCC ACCATGGAAC AACACTAAAC GTCTAGCTAC AATTCATGAG 
CTTATGCACT TTTGTACGAG TGATGTCTTG AGCTGGAATG AAGTAGAACG AGCCTTCAAG 
GTGTTTACTT TTGAGAGCCT GAAGCTCTCT GAGGTTGATG AAAAAGGGAA ACTGAAACCT 
TCTGGGATGA TGTGTGGGTC AGATTCTGAA ATGTTCAACA TACCGTGGGA CAACCCTGCC 
AGATTTGCTA AACAGATAAG GCAGCAATAT GTCATGAAAA TGAATACTCA AGAGGCCAAG 
CAGAAAGCAG ATATTAAAAT CAAAGACAGA ACACTATTTG TGGATCAGAA TTTGTCAATG 
TCTGTGCAAG ATAATGAAAG CAACCGAGAA CCTTCAGATC CTAGTCAGTG TGATGCTAAC 
AATATGAAGC ATTCTGACTT GAATAATCTC AAACTCTCAG TCCCTGATAA TAGACAGCTG 
TTAGAGCAGG AGAGCATCAT GAAGGCTCAG CCCCAACATG AGTCTCTGGA GCAGACCACA 
AACAATGAGA TCAAAGATGA TGCAGTCACA AAGGCTGATT CTCATGAAAA GAAACCCAAG 
AAGATGATGG TGGAAGCAGA TTTAGAGGAC ATAAAGAAAA CACAGCAGCG CAGTCTAATG 
GACTGGAGTT TTACTGAACA TTTTAAACCG AAAGTACTGC TTCAGGTCCT TCAAGAAGCC 
CATAAGCAAT ATAGGTGTGT TGATTCTTAC TACCACACCC AAGACAACTC TTTACTTTTA 
GTCTTTCACA ATCCAATGAA TAGACAACGT TTGCATTGTG AATATTGGAA CATTGCTCTC 
CACTCCAATG TTGGATTCAG GAATTATTTG GAACTTGTTG CAAAATCTAT TCAAGATTGG 
ATTACAAAAG AAGAAGCTAT ATATCAGGAA TCTAAAATGA ATGAGAAAAT CATCAGGACC 
AGAGCTGAGC TGGAATTGAA ATCTTCTGCT AATGCCAAAC TTACTTCTGC TAGCAAAATT 
TTTTCCATTA AAGAATCTAA AAGTAACAAA GGAATCAGCA AAACAGAGAT ATCAGATCAA 
GAAAAAGAAA AAGAGAAGGA AAAGATTCCT TTCATTTTAG AAGGCTCTCT CAAGGCATGG 
AAAGAAGAGC AACATCGATT AGCAGAAGAG GAGCGCTTAA GGGAAGAAAA GAAAGCAGAG 
AAGAAGGGTA AAGAAGCTGG TAAAAAGAAA GGCAAGGATA ACGCAGAGAA AGAGGATAGT 
AGGTCTTTGA AGAAAAAATC ACCTTACAAG GAGAAATCTA AAGAAGAACA AGTCAAGATC 
CAAGAAGTAA CAGAAGAGTC CCCCCACCAA CCAGAACCTA AGATAACTTA CCCGTTTCAC 
GGATACAATA TGGGAAATAT ACCCACTCAA ATCTCAGGGT CAAATTACTA CCTGTATCCT 
TCTGATGGGG GGCAGATTGA AGTGGAAAAG ACAATGTTTG AAAAAGGCCC AACTTTTATC 
AAAGTGAGAG TGGTAAAGGA CAACCACAAT TTTATGATTC ATTTAAATGA CCCTAAGGAA 
ATTGTGAAAA AGGAAGAGAA AGGGGATTAT TATTTAGAAG AGGAAGAAGA AGGAGATGAG 
GAACAAAGTC TTGAAACGGA AGTATCAGAT GCAAAGAATA AAGCTTTCAG CAAGTTTGGA 
TCTTTTTCTG CCACCTTAGA AAATGGAATC TGCCTCTCGA TAAGTTACTA TGGATCAAAT 
GGAATGGCAC CAGAAGATAA GGATCCTGAT TTAGAAACAA TATTGAATAT CCCTTCAGCA 
CTCACTCCAA CAGTGGTTCC TGTTATAGTG ACCGTTCCTC AAAGCAAAGC TAAAGGGAAA 
ATAAAAGGCA AAGAAAAACC CAAAGAATCC CTTAAAGAAG AAGAACACCC AAAAGAAGAA 
GAGAAAAAGG AAGAAGAAGT AGAACCAGAA CCTGTTTTAC AAGAGACTTT GGATGTTCCC 
ACCTTCCAGA GCCTAAATGT GTCTTGCCCC AGTGGGCTCC TGTTGACTTT CATTGGACAA 
GAATCTACAG GTCAATATGT TATAGATGAG GAACCCACCT GGGACATCAT GGTCCGTCAG 
AGCTACCCCC AGAGGGTGAA GCACTATGAG TTCTATAAAA CGGTGATGCC ACCCGCAGAG 
CAGGAGGCTT CAAGGGTTAT CACCAGTCAA GGCACTGTTG TCAAATATAT GTTGGATGGA 
TCCACACAGA TTCTCTTTGC AGATGGTGCT GTGAGCAGGA GTCCCAATTC AGGTCTTATT 
TGTCCTCCTT CTGAAATGCC AGCAACGCCT CACAGTGGAG ATTTGATGGA CTCTATTTCT 
CAGCAGAAAT CAGAAACGAT ACCATCTGAG ATTACCAACA CAAAGAAAGG AAAAAGTCAC 
AAAAGTCAGT CATCAATGGC CCATAAGGGT GAAATCCATG ACCCTCCTCC AGAGGCAGTT 
CAAACTGTAA CTCCTGTGGA GGTTCACATA GGCACCTGGT TTACAACCAC ACCTGAAGGA 
AATCGGATCG GCACCAAAGG ATTAGAAAGA ATAGCAGACT TGACCCCATT GTTATCCTTT 
CAGGCCACAG ATCCTGTCAA TGGAACGGTT ATGACAACTC GAGAAGACAA AGTTGTCATA 
GTTGAAAGGA AAGATGGTAC TCGGATAGTG GATCATGCTG ATGGTACCAG AATCACAACC 
TTTTATCAAG TTTATGAAGA TCAAATTATT CTGCCAGATG ATCAAGAAAC AACCGAGGGT 
CCTCGGACTG TCACCAGGCA GGTGAAGTGT ATGCGGGTAG AAAGCTCACG CTATGCCACT 
GTTATCGCCA ACTGTGAGGA CAGTAGCTGC TGTGCCACCT TTGGAGATGG AACAACTATT 
ATTGCAAAGC CACAGGGAAC ATACCAGGTG TTACCTCCAA ACACAGGCTC TCTTTATATT 
GACAAGGATT GTTCAGCTGT GTACTGCCAT GAGTCAAGCA GTAATATATA CTATCCTTTT 
CAAAAGCGTG AGCAGCTGCG AGCTGGCAGG TACATCATGA GGCATACTTC AGAGGTTATC 
TGTGAGGTTC TGGATCCTGA GGGAAACACT TTTCAGGTCA TGGCTGATGG TAGCATATCA 
ACTATATTAC CTGAAAAAAA ATTGGAAGAT GATTTAAATG AGAAAACTGA GGGCTATGAT 
AGTCTGTCCT CTATGCACCT TGAAAAGAAT CATCAGCAAA TCTATGGTGA ACATGTCCCC 
AGGTTTTTTG TTATGTATGC TGATGGATCA GGAATGGAAC TTCTTCGAGA CAGTGACATA 
GAAGAATATC TATCTTTGGC ATATAAAGAA TCAAATACTG TTGTTCTCCA AGAGCCAGTG 
CAGGAACAGC CAGGCACCCT AACCATCACA GTCCTTCGCC CTTTCCATGA AGCATCACCA 
TGGCAAGTAA AAAAGGAAGA TACAATTGTC CCTCCTAATC TCCGGTCAAG GTCATGGGAA 
ACATTTCCCT CAGTTGAGAA AAAAACTCCA GGACCTCCGT TTGGTACTCA GATTTGGAAA 
GGCCTTTGCA TTGAGTCCAA ACAGCTAGTG AGTGCCCCGG GTGCCATACT CAAGAGCCCC 
AGTGTGCTAC AGATGCGCCA ATTCATTCAG CATGAGGTCA TAAAGAATGA GGTGAAACTG 
AGGCTGCAGG TTTCCCTTAA GGATTACATA AACTATATTC TAAAGAAAGA AGATGAGCTG 
CAGGAAATGA TGGTTAAAGA TTCCAGAACT GAGGAGGAGA GAGGCAATGC TGCTGATCTC 
CTCAAGCTGG TTATGTCTTT CCCTAAAATG GAGGAAACTA CAAAAAGTCA TGTTACTGAA 
GTTGCAGCTC ACCTAACTGA TTTATTCAAG CAGTCTTTGG CTACGCCTCC AAAATGCCCA 
CCAGACACAT TTGGTAAAGA TTTCTTTGAA AAGACATGGA GACACACAGC ATCCTCAAAA 
CGCTGGAAAG AAAAGATAGA CAAAACGAGG AAGGAAATTG AGACAACACA GAATTACCTA 
ATGGATATTA AGAACCGCAT AATACCACCC TTTTTTAAAT CTGAATTGAA CCAGTTATAT 
CAGTCTCAGT ATAATCACCT GGACAGTCTT TCCAAAAAAC TGCCTTCTTT TACAAAGAAA 
AATGAAGATG CAAACGAAAC AGCTGTTCAA GATACATCTG ATCTTAATCT AGATTTCAAG 
CCACATAAGG TTTCAGAACA GAAATCCTCA AGTGTGCCTA GTCTTCCAAA ACCAGAGATT 
TCTGCAGATA AGAAGGATTT CACTGCTCAG AACCAAACTG AAAATTTAAC AAAATCTCCT 
GAAGAAGCAG AATCTTATGA GCCCGTGAAA ATTCCAACCC AGTCCTTGCT GCAGGATGTT 
GCGGGACAAA CAAGAAAAGA AAAAGTGAAG TTGCCTCATT ATTTGCTGAG TTCCAAGCCT 
AAGTCTCAAC CTCTTGCAAA GGTGCAAGAT TCTGTTGGAG GAAAAGTGAA CACATCCTCT 
GTTGCATCTG CTGCCATTAA TAATGCAAAG TCATCCCTTT TTGGGTTCCA TCTTCTCCCA 
TCATCAGTCA AGTTTGGAGT GCTTAAGGAA GGACATACCT ATGCCACAGT TGTAAAGCTC 
AAGAATGTTG GAGTGGACTT CTGCAGGTTT AAAGTAAAGC AGCCCCCACC CAGCACAGGA 
CTGAAAGTGA CTTACAAACC TGGACCTGTG GCAGCTGGTA TGCAGACAGA ACTGAATATA 
GAGTTATTTG CCACAGCTGT TGGAGAGGAT GGGGCCAAGG GATCAGCACA CATCTCTCAC 
AATATCGAGA TTATGACAGA GCATGAGGTT CTGTTCCTAC CTGTGGAAGC AACTGTTTTA 
ACAAGCAGCA ATTATGATAA ACGACCAAAA GACTTTCCCC AGGGAAAAGA AAATCCAATG 
GTCCAGAGAA CTTCTACAAT TTATTCCTCC ACACTTGGAG TCTTCATGTC TCGTAAAGTT 
TCTCCACATT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_996879.1
CDS	113..6784
Translation	MAPKKEKGGTVNTSSKIWEPSLIAAQFNQNDWQASIAFVVGNQIEDDLLIQALTVAVQVPQRKLFSMVSWQDILQQINEINTLVGSASSKKAKKPVGGNAPLYYEVLTAAKAIMDSGEKLTLPLIGKLLKFQLLQIKFKDQQRRENEKKVIEDKPKLEKDKGKAKSPKEKKAPSAKPAKGKGKDQPEANAPVKKTTQLKRRGEDDHTNRYIDDEPDDGAQHYIIVVGFNNPQLLAIMAELGIPITSVIKISSENYEPLQTHLAAVNQQQEVLLQSEDLEAEKLKKENAIKELKTFWKYLEPVLNNEKPETNLFDVARLEYMVKAADFPSDWSDGEMMLKLGTDIFENIACLMYDILDWKRQHQHYLESMQLINVPQVVNEKPVLEAMPTSEAPQPAVPAPGKKKAQYEEPQAPPPVTSVITTEVDMRYYNYLLNPIREEFISVPLILHCMLEQVVATEEDLVPPSLREPSPRADGLDHRIAAHIVSLLPSLCLSEREKKNLHDIFLSEEENESKAVPKGPLLLNYHDAHAHKKYALQDQKNFDPVQIEQEMQSKLPLWEFLQFPLPPPWNNTKRLATIHELMHFCTSDVLSWNEVERAFKVFTFESLKLSEVDEKGKLKPSGMMCGSDSEMFNIPWDNPARFAKQIRQQYVMKMNTQEAKQKADIKIKDRTLFVDQNLSMSVQDNESNREPSDPSQCDANNMKHSDLNNLKLSVPDNRQLLEQESIMKAQPQHESLEQTTNNEIKDDAVTKADSHEKKPKKMMVEADLEDIKKTQQRSLMDWSFTEHFKPKVLLQVLQEAHKQYRCVDSYYHTQDNSLLLVFHNPMNRQRLHCEYWNIALHSNVGFRNYLELVAKSIQDWITKEEAIYQESKMNEKIIRTRAELELKSSANAKLTSASKIFSIKESKSNKGISKTEISDQEKEKEKEKIPFILEGSLKAWKEEQHRLAEEERLREEKKAEKKGKEAGKKKGKDNAEKEDSRSLKKKSPYKEKSKEEQVKIQEVTEESPHQPEPKITYPFHGYNMGNIPTQISGSNYYLYPSDGGQIEVEKTMFEKGPTFIKVRVVKDNHNFMIHLNDPKEIVKKEEKGDYYLEEEEEGDEEQSLETEVSDAKNKAFSKFGSFSATLENGICLSISYYGSNGMAPEDKDPDLETILNIPSALTPTVVPVIVTVPQSKAKGKIKGKEKPKESLKEEEHPKEEEKKEEEVEPEPVLQETLDVPTFQSLNVSCPSGLLLTFIGQESTGQYVIDEEPTWDIMVRQSYPQRVKHYEFYKTVMPPAEQEASRVITSQGTVVKYMLDGSTQILFADGAVSRSPNSGLICPPSEMPATPHSGDLMDSISQQKSETIPSEITNTKKGKSHKSQSSMAHKGEIHDPPPEAVQTVTPVEVHIGTWFTTTPEGNRIGTKGLERIADLTPLLSFQATDPVNGTVMTTREDKVVIVERKDGTRIVDHADGTRITTFYQVYEDQIILPDDQETTEGPRTVTRQVKCMRVESSRYATVIANCEDSSCCATFGDGTTIIAKPQGTYQVLPPNTGSLYIDKDCSAVYCHESSSNIYYPFQKREQLRAGRYIMRHTSEVICEVLDPEGNTFQVMADGSISTILPEKKLEDDLNEKTEGYDSLSSMHLEKNHQQIYGEHVPRFFVMYADGSGMELLRDSDIEEYLSLAYKESNTVVLQEPVQEQPGTLTITVLRPFHEASPWQVKKEDTIVPPNLRSRSWETFPSVEKKTPGPPFGTQIWKGLCIESKQLVSAPGAILKSPSVLQMRQFIQHEVIKNEVKLRLQVSLKDYINYILKKEDELQEMMVKDSRTEEERGNAADLLKLVMSFPKMEETTKSHVTEVAAHLTDLFKQSLATPPKCPPDTFGKDFFEKTWRHTASSKRWKEKIDKTRKEIETTQNYLMDIKNRIIPPFFKSELNQLYQSQYNHLDSLSKKLPSFTKKNEDANETAVQDTSDLNLDFKPHKVSEQKSSSVPSLPKPEISADKKDFTAQNQTENLTKSPEEAESYEPVKIPTQSLLQDVAGQTRKEKVKLPHYLLSSKPKSQPLAKVQDSVGGKVNTSSVASAAINNAKSSLFGFHLLPSSVKFGVLKEGHTYATVVKLKNVGVDFCRFKVKQPPPSTGLKVTYKPGPVAAGMQTELNIELFATAVGEDGAKGSAHISHNIEIMTEHEVLFLPVEATVLTSSNYDKRPKDFPQGKENPMVQRTSTIYSSTLGVFMSRKVSPH

Target ORF information:

RefSeq Version	NM_206996.3
Organism	Homo sapiens(human)
Definition	Homo sapiens sperm associated antigen 17 (SPAG17), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_206996.3

ORF Insert Sequence:

ATGGCACCCA AGAAGGAGAA AGGAGGAACT GTGAACACCA GTTCTAAGAT ATGGGAACCC 
TCGCTCATAG CTGCACAGTT CAATCAGAAC GATTGGCAGG CCTCCATTGC TTTTGTGGTT 
GGGAACCAGA TTGAAGATGA TCTTCTCATC CAAGCCCTTA CCGTGGCTGT CCAGGTCCCT 
CAGCGTAAAC TCTTCAGTAT GGTGTCGTGG CAAGACATTC TCCAGCAGAT TAATGAAATA 
AATACACTTG TTGGATCTGC TTCATCTAAA AAGGCAAAAA AACCTGTAGG TGGTAATGCT 
CCTTTATATT ATGAGGTGTT AACGGCAGCA AAAGCAATTA TGGATAGTGG AGAGAAATTA 
ACCTTACCAC TGATAGGGAA ACTCTTGAAA TTTCAACTTC TCCAGATTAA ATTTAAGGAC 
CAACAGCGAC GGGAAAATGA AAAGAAGGTA ATAGAAGACA AACCTAAGTT AGAAAAGGAT 
AAAGGGAAAG CAAAATCTCC CAAGGAGAAA AAGGCTCCAA GTGCCAAGCC TGCCAAAGGA 
AAGGGAAAGG ATCAGCCTGA GGCAAATGCA CCAGTGAAAA AGACCACCCA GTTAAAGCGG 
AGAGGAGAAG ACGACCACAC CAATCGTTAC ATTGACGATG AGCCAGATGA TGGTGCCCAA 
CATTACATTA TAGTTGTGGG CTTTAACAAT CCTCAGCTAT TAGCAATTAT GGCTGAGCTT 
GGCATTCCTA TAACCAGCGT GATTAAAATA TCTTCAGAGA ATTATGAACC TCTGCAGACA 
CACCTGGCAG CAGTTAACCA GCAGCAGGAA GTTCTTCTTC AGTCAGAAGA TCTAGAAGCA 
GAAAAATTGA AGAAAGAAAA TGCCATAAAA GAGCTTAAAA CTTTCTGGAA GTACTTGGAA 
CCAGTCCTGA ATAATGAGAA ACCTGAAACA AATCTCTTTG ATGTTGCTCG ACTTGAGTAC 
ATGGTCAAAG CAGCTGATTT TCCTTCTGAC TGGTCAGATG GTGAGATGAT GCTGAAATTG 
GGCACTGATA TTTTTGAAAA TATTGCCTGC TTGATGTATG ACATCCTGGA TTGGAAAAGG 
CAGCACCAGC ACTATTTGGA AAGCATGCAG CTTATTAATG TTCCACAAGT GGTTAATGAG 
AAACCTGTAT TAGAAGCCAT GCCAACTTCA GAGGCTCCAC AACCTGCTGT ACCAGCTCCT 
GGAAAGAAGA AAGCACAGTA TGAAGAACCG CAAGCTCCAC CACCAGTGAC TTCAGTCATC 
ACAACTGAAG TAGACATGAG ATATTACAAT TATTTGCTGA ATCCAATTCG AGAGGAATTC 
ATTTCTGTGC CCCTGATACT GCATTGTATG CTGGAACAGG TTGTTGCAAC TGAAGAAGAT 
CTCGTCCCAC CCAGTCTGCG GGAGCCATCC CCCAGAGCAG ACGGGCTAGA CCACAGAATC 
GCAGCTCACA TTGTGTCCCT TCTGCCCTCA CTCTGTCTCT CAGAGAGGGA GAAAAAGAAT 
CTTCATGACA TATTTTTATC TGAAGAAGAA AATGAAAGCA AAGCAGTGCC CAAAGGCCCC 
CTCCTACTGA ACTATCATGA TGCACACGCC CACAAGAAGT ACGCACTACA GGACCAAAAG 
AATTTTGATC CAGTTCAAAT TGAGCAGGAG ATGCAGTCCA AGTTGCCACT GTGGGAATTT 
CTTCAATTCC CTCTACCCCC ACCATGGAAC AACACTAAAC GTCTAGCTAC AATTCATGAG 
CTTATGCACT TTTGTACGAG TGATGTCTTG AGCTGGAATG AAGTAGAACG AGCCTTCAAG 
GTGTTTACTT TTGAGAGCCT GAAGCTCTCT GAGGTTGATG AAAAAGGGAA ACTGAAACCT 
TCTGGGATGA TGTGTGGGTC AGATTCTGAA ATGTTCAACA TACCGTGGGA CAACCCTGCC 
AGATTTGCTA AACAGATAAG GCAGCAATAT GTCATGAAAA TGAATACTCA AGAGGCCAAG 
CAGAAAGCAG ATATTAAAAT CAAAGACAGA ACACTATTTG TGGATCAGAA TTTGTCAATG 
TCTGTGCAAG ATAATGAAAG CAACCGAGAA CCTTCAGATC CTAGTCAGTG TGATGCTAAC 
AATATGAAGC ATTCTGACTT GAATAATCTC AAACTCTCAG TCCCTGATAA TAGACAGCTG 
TTAGAGCAGG AGAGCATCAT GAAGGCTCAG CCCCAACATG AGTCTCTGGA GCAGACCACA 
AACAATGAGA TCAAAGATGA TGCAGTCACA AAGGCTGATT CTCATGAAAA GAAACCCAAG 
AAGATGATGG TGGAAGCAGA TTTAGAGGAC ATAAAGAAAA CACAGCAGCG CAGTCTAATG 
GACTGGAGTT TTACTGAACA TTTTAAACCG AAAGTACTGC TTCAGGTCCT TCAAGAAGCC 
CATAAGCAAT ATAGGTGTGT TGATTCTTAC TACCACACCC AAGACAACTC TTTACTTTTA 
GTCTTTCACA ATCCAATGAA TAGACAACGT TTGCATTGTG AATATTGGAA CATTGCTCTC 
CACTCCAATG TTGGATTCAG GAATTATTTG GAACTTGTTG CAAAATCTAT TCAAGATTGG 
ATTACAAAAG AAGAAGCTAT ATATCAGGAA TCTAAAATGA ATGAGAAAAT CATCAGGACC 
AGAGCTGAGC TGGAATTGAA ATCTTCTGCT AATGCCAAAC TTACTTCTGC TAGCAAAATT 
TTTTCCATTA AAGAATCTAA AAGTAACAAA GGAATCAGCA AAACAGAGAT ATCAGATCAA 
GAAAAAGAAA AAGAGAAGGA AAAGATTCCT TTCATTTTAG AAGGCTCTCT CAAGGCATGG 
AAAGAAGAGC AACATCGATT AGCAGAAGAG GAGCGCTTAA GGGAAGAAAA GAAAGCAGAG 
AAGAAGGGTA AAGAAGCTGG TAAAAAGAAA GGCAAGGATA ACGCAGAGAA AGAGGATAGT 
AGGTCTTTGA AGAAAAAATC ACCTTACAAG GAGAAATCTA AAGAAGAACA AGTCAAGATC 
CAAGAAGTAA CAGAAGAGTC CCCCCACCAA CCAGAACCTA AGATAACTTA CCCGTTTCAC 
GGATACAATA TGGGAAATAT ACCCACTCAA ATCTCAGGGT CAAATTACTA CCTGTATCCT 
TCTGATGGGG GGCAGATTGA AGTGGAAAAG ACAATGTTTG AAAAAGGCCC AACTTTTATC 
AAAGTGAGAG TGGTAAAGGA CAACCACAAT TTTATGATTC ATTTAAATGA CCCTAAGGAA 
ATTGTGAAAA AGGAAGAGAA AGGGGATTAT TATTTAGAAG AGGAAGAAGA AGGAGATGAG 
GAACAAAGTC TTGAAACGGA AGTATCAGAT GCAAAGAATA AAGCTTTCAG CAAGTTTGGA 
TCTTTTTCTG CCACCTTAGA AAATGGAATC TGCCTCTCGA TAAGTTACTA TGGATCAAAT 
GGAATGGCAC CAGAAGATAA GGATCCTGAT TTAGAAACAA TATTGAATAT CCCTTCAGCA 
CTCACTCCAA CAGTGGTTCC TGTTATAGTG ACCGTTCCTC AAAGCAAAGC TAAAGGGAAA 
ATAAAAGGCA AAGAAAAACC CAAAGAATCC CTTAAAGAAG AAGAACACCC AAAAGAAGAA 
GAGAAAAAGG AAGAAGAAGT AGAACCAGAA CCTGTTTTAC AAGAGACTTT GGATGTTCCC 
ACCTTCCAGA GCCTAAATGT GTCTTGCCCC AGTGGGCTCC TGTTGACTTT CATTGGACAA 
GAATCTACAG GTCAATATGT TATAGATGAG GAACCCACCT GGGACATCAT GGTCCGTCAG 
AGCTACCCCC AGAGGGTGAA GCACTATGAG TTCTATAAAA CGGTGATGCC ACCCGCAGAG 
CAGGAGGCTT CAAGGGTTAT CACCAGTCAA GGCACTGTTG TCAAATATAT GTTGGATGGA 
TCCACACAGA TTCTCTTTGC AGATGGTGCT GTGAGCAGGA GTCCCAATTC AGGTCTTATT 
TGTCCTCCTT CTGAAATGCC AGCAACGCCT CACAGTGGAG ATTTGATGGA CTCTATTTCT 
CAGCAGAAAT CAGAAACGAT ACCATCTGAG ATTACCAACA CAAAGAAAGG AAAAAGTCAC 
AAAAGTCAGT CATCAATGGC CCATAAGGGT GAAATCCATG ACCCTCCTCC AGAGGCAGTT 
CAAACTGTAA CTCCTGTGGA GGTTCACATA GGCACCTGGT TTACAACCAC ACCTGAAGGA 
AATCGGATCG GCACCAAAGG ATTAGAAAGA ATAGCAGACT TGACCCCATT GTTATCCTTT 
CAGGCCACAG ATCCTGTCAA TGGAACGGTT ATGACAACTC GAGAAGACAA AGTTGTCATA 
GTTGAAAGGA AAGATGGTAC TCGGATAGTG GATCATGCTG ATGGTACCAG AATCACAACC 
TTTTATCAAG TTTATGAAGA TCAAATTATT CTGCCAGATG ATCAAGAAAC AACCGAGGGT 
CCTCGGACTG TCACCAGGCA GGTGAAGTGT ATGCGGGTAG AAAGCTCACG CTATGCCACT 
GTTATCGCCA ACTGTGAGGA CAGTAGCTGC TGTGCCACCT TTGGAGATGG AACAACTATT 
ATTGCAAAGC CACAGGGAAC ATACCAGGTG TTACCTCCAA ACACAGGCTC TCTTTATATT 
GACAAGGATT GTTCAGCTGT GTACTGCCAT GAGTCAAGCA GTAATATATA CTATCCTTTT 
CAAAAGCGTG AGCAGCTGCG AGCTGGCAGG TACATCATGA GGCATACTTC AGAGGTTATC 
TGTGAGGTTC TGGATCCTGA GGGAAACACT TTTCAGGTCA TGGCTGATGG TAGCATATCA 
ACTATATTAC CTGAAAAAAA ATTGGAAGAT GATTTAAATG AGAAAACTGA GGGCTATGAT 
AGTCTGTCCT CTATGCACCT TGAAAAGAAT CATCAGCAAA TCTATGGTGA ACATGTCCCC 
AGGTTTTTTG TTATGTATGC TGATGGATCA GGAATGGAAC TTCTTCGAGA CAGTGACATA 
GAAGAATATC TATCTTTGGC ATATAAAGAA TCAAATACTG TTGTTCTCCA AGAGCCAGTG 
CAGGAACAGC CAGGCACCCT AACCATCACA GTCCTTCGCC CTTTCCATGA AGCATCACCA 
TGGCAAGTAA AAAAGGAAGA TACAATTGTC CCTCCTAATC TCCGGTCAAG GTCATGGGAA 
ACATTTCCCT CAGTTGAGAA AAAAACTCCA GGACCTCCGT TTGGTACTCA GATTTGGAAA 
GGCCTTTGCA TTGAGTCCAA ACAGCTAGTG AGTGCCCCGG GTGCCATACT CAAGAGCCCC 
AGTGTGCTAC AGATGCGCCA ATTCATTCAG CATGAGGTCA TAAAGAATGA GGTGAAACTG 
AGGCTGCAGG TTTCCCTTAA GGATTACATA AACTATATTC TAAAGAAAGA AGATGAGCTG 
CAGGAAATGA TGGTTAAAGA TTCCAGAACT GAGGAGGAGA GAGGCAATGC TGCTGATCTC 
CTCAAGCTGG TTATGTCTTT CCCTAAAATG GAGGAAACTA CAAAAAGTCA TGTTACTGAA 
GTTGCAGCTC ACCTAACTGA TTTATTCAAG CAGTCTTTGG CTACGCCTCC AAAATGCCCA 
CCAGACACAT TTGGTAAAGA TTTCTTTGAA AAGACATGGA GACACACAGC ATCCTCAAAA 
CGCTGGAAAG AAAAGATAGA CAAAACGAGG AAGGAAATTG AGACAACACA GAATTACCTA 
ATGGATATTA AGAACCGCAT AATACCACCC TTTTTTAAAT CTGAATTGAA CCAGTTATAT 
CAGTCTCAGT ATAATCACCT GGACAGTCTT TCCAAAAAAC TGCCTTCTTT TACAAAGAAA 
AATGAAGATG CAAACGAAAC AGCTGTTCAA GATACATCTG ATCTTAATCT AGATTTCAAG 
CCACATAAGG TTTCAGAACA GAAATCCTCA AGTGTGCCTA GTCTTCCAAA ACCAGAGATT 
TCTGCAGATA AGAAGGATTT CACTGCTCAG AACCAAACTG AAAATTTAAC AAAATCTCCT 
GAAGAAGCAG AATCTTATGA GCCCGTGAAA ATTCCAACCC AGTCCTTGCT GCAGGATGTT 
GCGGGACAAA CAAGAAAAGA AAAAGTGAAG TTGCCTCATT ATTTGCTGAG TTCCAAGCCT 
AAGTCTCAAC CTCTTGCAAA GGTGCAAGAT TCTGTTGGAG GAAAAGTGAA CACATCCTCT 
GTTGCATCTG CTGCCATTAA TAATGCAAAG TCATCCCTTT TTGGGTTCCA TCTTCTCCCA 
TCATCAGTCA AGTTTGGAGT GCTTAAGGAA GGACATACCT ATGCCACAGT TGTAAAGCTC 
AAGAATGTTG GAGTGGACTT CTGCAGGTTT AAAGTAAAGC AGCCCCCACC CAGCACAGGA 
CTGAAAGTGA CTTACAAACC TGGACCTGTG GCAGCTGGTA TGCAGACAGA ACTGAATATA 
GAGTTATTTG CCACAGCTGT TGGAGAGGAT GGGGCCAAGG GATCAGCACA CATCTCTCAC 
AATATCGAGA TTATGACAGA GCATGAGGTT CTGTTCCTAC CTGTGGAAGC AACTGTTTTA 
ACAAGCAGCA ATTATGATAA ACGACCAAAA GACTTTCCCC AGGGAAAAGA AAATCCAATG 
GTCCAGAGAA CTTCTACAAT TTATTCCTCC ACACTTGGAG TCTTCATGTC TCGTAAAGTT 
TCTCCACATT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPAG17 ( NM_206996.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_996879.1 Homo sapiens sperm associated antigen 17 (SPAG17), mRNA.

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CloneID	OHu16428
Clone ID Related Accession (Same CDS sequence)	NM_206996.3 , NM_206996.4
Accession Version	NM_206996.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6672bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-25
Organism	Homo sapiens(human)
Product	sperm-associated antigen 17
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL513191.4, DB060943.1, AY555274.1, DB340825.1 and AL121993.7. This sequence is a reference standard in the RefSeqGene project. On May 17, 2019 this sequence version replaced NM_206996.3. ##Evidence-Data-START## Transcript exon combination :: AY555274.1, BX648110.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1966682, SAMEA1968189 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000336338.10/ ENSP00000337804.5 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCACCCA AGAAGGAGAA AGGAGGAACT GTGAACACCA GTTCTAAGAT ATGGGAACCC 
TCGCTCATAG CTGCACAGTT CAATCAGAAC GATTGGCAGG CCTCCATTGC TTTTGTGGTT 
GGGAACCAGA TTGAAGATGA TCTTCTCATC CAAGCCCTTA CCGTGGCTGT CCAGGTCCCT 
CAGCGTAAAC TCTTCAGTAT GGTGTCGTGG CAAGACATTC TCCAGCAGAT TAATGAAATA 
AATACACTTG TTGGATCTGC TTCATCTAAA AAGGCAAAAA AACCTGTAGG TGGTAATGCT 
CCTTTATATT ATGAGGTGTT AACGGCAGCA AAAGCAATTA TGGATAGTGG AGAGAAATTA 
ACCTTACCAC TGATAGGGAA ACTCTTGAAA TTTCAACTTC TCCAGATTAA ATTTAAGGAC 
CAACAGCGAC GGGAAAATGA AAAGAAGGTA ATAGAAGACA AACCTAAGTT AGAAAAGGAT 
AAAGGGAAAG CAAAATCTCC CAAGGAGAAA AAGGCTCCAA GTGCCAAGCC TGCCAAAGGA 
AAGGGAAAGG ATCAGCCTGA GGCAAATGCA CCAGTGAAAA AGACCACCCA GTTAAAGCGG 
AGAGGAGAAG ACGACCACAC CAATCGTTAC ATTGACGATG AGCCAGATGA TGGTGCCCAA 
CATTACATTA TAGTTGTGGG CTTTAACAAT CCTCAGCTAT TAGCAATTAT GGCTGAGCTT 
GGCATTCCTA TAACCAGCGT GATTAAAATA TCTTCAGAGA ATTATGAACC TCTGCAGACA 
CACCTGGCAG CAGTTAACCA GCAGCAGGAA GTTCTTCTTC AGTCAGAAGA TCTAGAAGCA 
GAAAAATTGA AGAAAGAAAA TGCCATAAAA GAGCTTAAAA CTTTCTGGAA GTACTTGGAA 
CCAGTCCTGA ATAATGAGAA ACCTGAAACA AATCTCTTTG ATGTTGCTCG ACTTGAGTAC 
ATGGTCAAAG CAGCTGATTT TCCTTCTGAC TGGTCAGATG GTGAGATGAT GCTGAAATTG 
GGCACTGATA TTTTTGAAAA TATTGCCTGC TTGATGTATG ACATCCTGGA TTGGAAAAGG 
CAGCACCAGC ACTATTTGGA AAGCATGCAG CTTATTAATG TTCCACAAGT GGTTAATGAG 
AAACCTGTAT TAGAAGCCAT GCCAACTTCA GAGGCTCCAC AACCTGCTGT ACCAGCTCCT 
GGAAAGAAGA AAGCACAGTA TGAAGAACCG CAAGCTCCAC CACCAGTGAC TTCAGTCATC 
ACAACTGAAG TAGACATGAG ATATTACAAT TATTTGCTGA ATCCAATTCG AGAGGAATTC 
ATTTCTGTGC CCCTGATACT GCATTGTATG CTGGAACAGG TTGTTGCAAC TGAAGAAGAT 
CTCGTCCCAC CCAGTCTGCG GGAGCCATCC CCCAGAGCAG ACGGGCTAGA CCACAGAATC 
GCAGCTCACA TTGTGTCCCT TCTGCCCTCA CTCTGTCTCT CAGAGAGGGA GAAAAAGAAT 
CTTCATGACA TATTTTTATC TGAAGAAGAA AATGAAAGCA AAGCAGTGCC CAAAGGCCCC 
CTCCTACTGA ACTATCATGA TGCACACGCC CACAAGAAGT ACGCACTACA GGACCAAAAG 
AATTTTGATC CAGTTCAAAT TGAGCAGGAG ATGCAGTCCA AGTTGCCACT GTGGGAATTT 
CTTCAATTCC CTCTACCCCC ACCATGGAAC AACACTAAAC GTCTAGCTAC AATTCATGAG 
CTTATGCACT TTTGTACGAG TGATGTCTTG AGCTGGAATG AAGTAGAACG AGCCTTCAAG 
GTGTTTACTT TTGAGAGCCT GAAGCTCTCT GAGGTTGATG AAAAAGGGAA ACTGAAACCT 
TCTGGGATGA TGTGTGGGTC AGATTCTGAA ATGTTCAACA TACCGTGGGA CAACCCTGCC 
AGATTTGCTA AACAGATAAG GCAGCAATAT GTCATGAAAA TGAATACTCA AGAGGCCAAG 
CAGAAAGCAG ATATTAAAAT CAAAGACAGA ACACTATTTG TGGATCAGAA TTTGTCAATG 
TCTGTGCAAG ATAATGAAAG CAACCGAGAA CCTTCAGATC CTAGTCAGTG TGATGCTAAC 
AATATGAAGC ATTCTGACTT GAATAATCTC AAACTCTCAG TCCCTGATAA TAGACAGCTG 
TTAGAGCAGG AGAGCATCAT GAAGGCTCAG CCCCAACATG AGTCTCTGGA GCAGACCACA 
AACAATGAGA TCAAAGATGA TGCAGTCACA AAGGCTGATT CTCATGAAAA GAAACCCAAG 
AAGATGATGG TGGAAGCAGA TTTAGAGGAC ATAAAGAAAA CACAGCAGCG CAGTCTAATG 
GACTGGAGTT TTACTGAACA TTTTAAACCG AAAGTACTGC TTCAGGTCCT TCAAGAAGCC 
CATAAGCAAT ATAGGTGTGT TGATTCTTAC TACCACACCC AAGACAACTC TTTACTTTTA 
GTCTTTCACA ATCCAATGAA TAGACAACGT TTGCATTGTG AATATTGGAA CATTGCTCTC 
CACTCCAATG TTGGATTCAG GAATTATTTG GAACTTGTTG CAAAATCTAT TCAAGATTGG 
ATTACAAAAG AAGAAGCTAT ATATCAGGAA TCTAAAATGA ATGAGAAAAT CATCAGGACC 
AGAGCTGAGC TGGAATTGAA ATCTTCTGCT AATGCCAAAC TTACTTCTGC TAGCAAAATT 
TTTTCCATTA AAGAATCTAA AAGTAACAAA GGAATCAGCA AAACAGAGAT ATCAGATCAA 
GAAAAAGAAA AAGAGAAGGA AAAGATTCCT TTCATTTTAG AAGGCTCTCT CAAGGCATGG 
AAAGAAGAGC AACATCGATT AGCAGAAGAG GAGCGCTTAA GGGAAGAAAA GAAAGCAGAG 
AAGAAGGGTA AAGAAGCTGG TAAAAAGAAA GGCAAGGATA ACGCAGAGAA AGAGGATAGT 
AGGTCTTTGA AGAAAAAATC ACCTTACAAG GAGAAATCTA AAGAAGAACA AGTCAAGATC 
CAAGAAGTAA CAGAAGAGTC CCCCCACCAA CCAGAACCTA AGATAACTTA CCCGTTTCAC 
GGATACAATA TGGGAAATAT ACCCACTCAA ATCTCAGGGT CAAATTACTA CCTGTATCCT 
TCTGATGGGG GGCAGATTGA AGTGGAAAAG ACAATGTTTG AAAAAGGCCC AACTTTTATC 
AAAGTGAGAG TGGTAAAGGA CAACCACAAT TTTATGATTC ATTTAAATGA CCCTAAGGAA 
ATTGTGAAAA AGGAAGAGAA AGGGGATTAT TATTTAGAAG AGGAAGAAGA AGGAGATGAG 
GAACAAAGTC TTGAAACGGA AGTATCAGAT GCAAAGAATA AAGCTTTCAG CAAGTTTGGA 
TCTTTTTCTG CCACCTTAGA AAATGGAATC TGCCTCTCGA TAAGTTACTA TGGATCAAAT 
GGAATGGCAC CAGAAGATAA GGATCCTGAT TTAGAAACAA TATTGAATAT CCCTTCAGCA 
CTCACTCCAA CAGTGGTTCC TGTTATAGTG ACCGTTCCTC AAAGCAAAGC TAAAGGGAAA 
ATAAAAGGCA AAGAAAAACC CAAAGAATCC CTTAAAGAAG AAGAACACCC AAAAGAAGAA 
GAGAAAAAGG AAGAAGAAGT AGAACCAGAA CCTGTTTTAC AAGAGACTTT GGATGTTCCC 
ACCTTCCAGA GCCTAAATGT GTCTTGCCCC AGTGGGCTCC TGTTGACTTT CATTGGACAA 
GAATCTACAG GTCAATATGT TATAGATGAG GAACCCACCT GGGACATCAT GGTCCGTCAG 
AGCTACCCCC AGAGGGTGAA GCACTATGAG TTCTATAAAA CGGTGATGCC ACCCGCAGAG 
CAGGAGGCTT CAAGGGTTAT CACCAGTCAA GGCACTGTTG TCAAATATAT GTTGGATGGA 
TCCACACAGA TTCTCTTTGC AGATGGTGCT GTGAGCAGGA GTCCCAATTC AGGTCTTATT 
TGTCCTCCTT CTGAAATGCC AGCAACGCCT CACAGTGGAG ATTTGATGGA CTCTATTTCT 
CAGCAGAAAT CAGAAACGAT ACCATCTGAG ATTACCAACA CAAAGAAAGG AAAAAGTCAC 
AAAAGTCAGT CATCAATGGC CCATAAGGGT GAAATCCATG ACCCTCCTCC AGAGGCAGTT 
CAAACTGTAA CTCCTGTGGA GGTTCACATA GGCACCTGGT TTACAACCAC ACCTGAAGGA 
AATCGGATCG GCACCAAAGG ATTAGAAAGA ATAGCAGACT TGACCCCATT GTTATCCTTT 
CAGGCCACAG ATCCTGTCAA TGGAACGGTT ATGACAACTC GAGAAGACAA AGTTGTCATA 
GTTGAAAGGA AAGATGGTAC TCGGATAGTG GATCATGCTG ATGGTACCAG AATCACAACC 
TTTTATCAAG TTTATGAAGA TCAAATTATT CTGCCAGATG ATCAAGAAAC AACCGAGGGT 
CCTCGGACTG TCACCAGGCA GGTGAAGTGT ATGCGGGTAG AAAGCTCACG CTATGCCACT 
GTTATCGCCA ACTGTGAGGA CAGTAGCTGC TGTGCCACCT TTGGAGATGG AACAACTATT 
ATTGCAAAGC CACAGGGAAC ATACCAGGTG TTACCTCCAA ACACAGGCTC TCTTTATATT 
GACAAGGATT GTTCAGCTGT GTACTGCCAT GAGTCAAGCA GTAATATATA CTATCCTTTT 
CAAAAGCGTG AGCAGCTGCG AGCTGGCAGG TACATCATGA GGCATACTTC AGAGGTTATC 
TGTGAGGTTC TGGATCCTGA GGGAAACACT TTTCAGGTCA TGGCTGATGG TAGCATATCA 
ACTATATTAC CTGAAAAAAA ATTGGAAGAT GATTTAAATG AGAAAACTGA GGGCTATGAT 
AGTCTGTCCT CTATGCACCT TGAAAAGAAT CATCAGCAAA TCTATGGTGA ACATGTCCCC 
AGGTTTTTTG TTATGTATGC TGATGGATCA GGAATGGAAC TTCTTCGAGA CAGTGACATA 
GAAGAATATC TATCTTTGGC ATATAAAGAA TCAAATACTG TTGTTCTCCA AGAGCCAGTG 
CAGGAACAGC CAGGCACCCT AACCATCACA GTCCTTCGCC CTTTCCATGA AGCATCACCA 
TGGCAAGTAA AAAAGGAAGA TACAATTGTC CCTCCTAATC TCCGGTCAAG GTCATGGGAA 
ACATTTCCCT CAGTTGAGAA AAAAACTCCA GGACCTCCGT TTGGTACTCA GATTTGGAAA 
GGCCTTTGCA TTGAGTCCAA ACAGCTAGTG AGTGCCCCGG GTGCCATACT CAAGAGCCCC 
AGTGTGCTAC AGATGCGCCA ATTCATTCAG CATGAGGTCA TAAAGAATGA GGTGAAACTG 
AGGCTGCAGG TTTCCCTTAA GGATTACATA AACTATATTC TAAAGAAAGA AGATGAGCTG 
CAGGAAATGA TGGTTAAAGA TTCCAGAACT GAGGAGGAGA GAGGCAATGC TGCTGATCTC 
CTCAAGCTGG TTATGTCTTT CCCTAAAATG GAGGAAACTA CAAAAAGTCA TGTTACTGAA 
GTTGCAGCTC ACCTAACTGA TTTATTCAAG CAGTCTTTGG CTACGCCTCC AAAATGCCCA 
CCAGACACAT TTGGTAAAGA TTTCTTTGAA AAGACATGGA GACACACAGC ATCCTCAAAA 
CGCTGGAAAG AAAAGATAGA CAAAACGAGG AAGGAAATTG AGACAACACA GAATTACCTA 
ATGGATATTA AGAACCGCAT AATACCACCC TTTTTTAAAT CTGAATTGAA CCAGTTATAT 
CAGTCTCAGT ATAATCACCT GGACAGTCTT TCCAAAAAAC TGCCTTCTTT TACAAAGAAA 
AATGAAGATG CAAACGAAAC AGCTGTTCAA GATACATCTG ATCTTAATCT AGATTTCAAG 
CCACATAAGG TTTCAGAACA GAAATCCTCA AGTGTGCCTA GTCTTCCAAA ACCAGAGATT 
TCTGCAGATA AGAAGGATTT CACTGCTCAG AACCAAACTG AAAATTTAAC AAAATCTCCT 
GAAGAAGCAG AATCTTATGA GCCCGTGAAA ATTCCAACCC AGTCCTTGCT GCAGGATGTT 
GCGGGACAAA CAAGAAAAGA AAAAGTGAAG TTGCCTCATT ATTTGCTGAG TTCCAAGCCT 
AAGTCTCAAC CTCTTGCAAA GGTGCAAGAT TCTGTTGGAG GAAAAGTGAA CACATCCTCT 
GTTGCATCTG CTGCCATTAA TAATGCAAAG TCATCCCTTT TTGGGTTCCA TCTTCTCCCA 
TCATCAGTCA AGTTTGGAGT GCTTAAGGAA GGACATACCT ATGCCACAGT TGTAAAGCTC 
AAGAATGTTG GAGTGGACTT CTGCAGGTTT AAAGTAAAGC AGCCCCCACC CAGCACAGGA 
CTGAAAGTGA CTTACAAACC TGGACCTGTG GCAGCTGGTA TGCAGACAGA ACTGAATATA 
GAGTTATTTG CCACAGCTGT TGGAGAGGAT GGGGCCAAGG GATCAGCACA CATCTCTCAC 
AATATCGAGA TTATGACAGA GCATGAGGTT CTGTTCCTAC CTGTGGAAGC AACTGTTTTA 
ACAAGCAGCA ATTATGATAA ACGACCAAAA GACTTTCCCC AGGGAAAAGA AAATCCAATG 
GTCCAGAGAA CTTCTACAAT TTATTCCTCC ACACTTGGAG TCTTCATGTC TCGTAAAGTT 
TCTCCACATT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_996879.1
CDS	72..6743
Translation	MAPKKEKGGTVNTSSKIWEPSLIAAQFNQNDWQASIAFVVGNQIEDDLLIQALTVAVQVPQRKLFSMVSWQDILQQINEINTLVGSASSKKAKKPVGGNAPLYYEVLTAAKAIMDSGEKLTLPLIGKLLKFQLLQIKFKDQQRRENEKKVIEDKPKLEKDKGKAKSPKEKKAPSAKPAKGKGKDQPEANAPVKKTTQLKRRGEDDHTNRYIDDEPDDGAQHYIIVVGFNNPQLLAIMAELGIPITSVIKISSENYEPLQTHLAAVNQQQEVLLQSEDLEAEKLKKENAIKELKTFWKYLEPVLNNEKPETNLFDVARLEYMVKAADFPSDWSDGEMMLKLGTDIFENIACLMYDILDWKRQHQHYLESMQLINVPQVVNEKPVLEAMPTSEAPQPAVPAPGKKKAQYEEPQAPPPVTSVITTEVDMRYYNYLLNPIREEFISVPLILHCMLEQVVATEEDLVPPSLREPSPRADGLDHRIAAHIVSLLPSLCLSEREKKNLHDIFLSEEENESKAVPKGPLLLNYHDAHAHKKYALQDQKNFDPVQIEQEMQSKLPLWEFLQFPLPPPWNNTKRLATIHELMHFCTSDVLSWNEVERAFKVFTFESLKLSEVDEKGKLKPSGMMCGSDSEMFNIPWDNPARFAKQIRQQYVMKMNTQEAKQKADIKIKDRTLFVDQNLSMSVQDNESNREPSDPSQCDANNMKHSDLNNLKLSVPDNRQLLEQESIMKAQPQHESLEQTTNNEIKDDAVTKADSHEKKPKKMMVEADLEDIKKTQQRSLMDWSFTEHFKPKVLLQVLQEAHKQYRCVDSYYHTQDNSLLLVFHNPMNRQRLHCEYWNIALHSNVGFRNYLELVAKSIQDWITKEEAIYQESKMNEKIIRTRAELELKSSANAKLTSASKIFSIKESKSNKGISKTEISDQEKEKEKEKIPFILEGSLKAWKEEQHRLAEEERLREEKKAEKKGKEAGKKKGKDNAEKEDSRSLKKKSPYKEKSKEEQVKIQEVTEESPHQPEPKITYPFHGYNMGNIPTQISGSNYYLYPSDGGQIEVEKTMFEKGPTFIKVRVVKDNHNFMIHLNDPKEIVKKEEKGDYYLEEEEEGDEEQSLETEVSDAKNKAFSKFGSFSATLENGICLSISYYGSNGMAPEDKDPDLETILNIPSALTPTVVPVIVTVPQSKAKGKIKGKEKPKESLKEEEHPKEEEKKEEEVEPEPVLQETLDVPTFQSLNVSCPSGLLLTFIGQESTGQYVIDEEPTWDIMVRQSYPQRVKHYEFYKTVMPPAEQEASRVITSQGTVVKYMLDGSTQILFADGAVSRSPNSGLICPPSEMPATPHSGDLMDSISQQKSETIPSEITNTKKGKSHKSQSSMAHKGEIHDPPPEAVQTVTPVEVHIGTWFTTTPEGNRIGTKGLERIADLTPLLSFQATDPVNGTVMTTREDKVVIVERKDGTRIVDHADGTRITTFYQVYEDQIILPDDQETTEGPRTVTRQVKCMRVESSRYATVIANCEDSSCCATFGDGTTIIAKPQGTYQVLPPNTGSLYIDKDCSAVYCHESSSNIYYPFQKREQLRAGRYIMRHTSEVICEVLDPEGNTFQVMADGSISTILPEKKLEDDLNEKTEGYDSLSSMHLEKNHQQIYGEHVPRFFVMYADGSGMELLRDSDIEEYLSLAYKESNTVVLQEPVQEQPGTLTITVLRPFHEASPWQVKKEDTIVPPNLRSRSWETFPSVEKKTPGPPFGTQIWKGLCIESKQLVSAPGAILKSPSVLQMRQFIQHEVIKNEVKLRLQVSLKDYINYILKKEDELQEMMVKDSRTEEERGNAADLLKLVMSFPKMEETTKSHVTEVAAHLTDLFKQSLATPPKCPPDTFGKDFFEKTWRHTASSKRWKEKIDKTRKEIETTQNYLMDIKNRIIPPFFKSELNQLYQSQYNHLDSLSKKLPSFTKKNEDANETAVQDTSDLNLDFKPHKVSEQKSSSVPSLPKPEISADKKDFTAQNQTENLTKSPEEAESYEPVKIPTQSLLQDVAGQTRKEKVKLPHYLLSSKPKSQPLAKVQDSVGGKVNTSSVASAAINNAKSSLFGFHLLPSSVKFGVLKEGHTYATVVKLKNVGVDFCRFKVKQPPPSTGLKVTYKPGPVAAGMQTELNIELFATAVGEDGAKGSAHISHNIEIMTEHEVLFLPVEATVLTSSNYDKRPKDFPQGKENPMVQRTSTIYSSTLGVFMSRKVSPH

Target ORF information:

RefSeq Version	NM_206996.4
Organism	Homo sapiens(human)
Definition	Homo sapiens sperm associated antigen 17 (SPAG17), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_206996.4

ORF Insert Sequence:

ATGGCACCCA AGAAGGAGAA AGGAGGAACT GTGAACACCA GTTCTAAGAT ATGGGAACCC 
TCGCTCATAG CTGCACAGTT CAATCAGAAC GATTGGCAGG CCTCCATTGC TTTTGTGGTT 
GGGAACCAGA TTGAAGATGA TCTTCTCATC CAAGCCCTTA CCGTGGCTGT CCAGGTCCCT 
CAGCGTAAAC TCTTCAGTAT GGTGTCGTGG CAAGACATTC TCCAGCAGAT TAATGAAATA 
AATACACTTG TTGGATCTGC TTCATCTAAA AAGGCAAAAA AACCTGTAGG TGGTAATGCT 
CCTTTATATT ATGAGGTGTT AACGGCAGCA AAAGCAATTA TGGATAGTGG AGAGAAATTA 
ACCTTACCAC TGATAGGGAA ACTCTTGAAA TTTCAACTTC TCCAGATTAA ATTTAAGGAC 
CAACAGCGAC GGGAAAATGA AAAGAAGGTA ATAGAAGACA AACCTAAGTT AGAAAAGGAT 
AAAGGGAAAG CAAAATCTCC CAAGGAGAAA AAGGCTCCAA GTGCCAAGCC TGCCAAAGGA 
AAGGGAAAGG ATCAGCCTGA GGCAAATGCA CCAGTGAAAA AGACCACCCA GTTAAAGCGG 
AGAGGAGAAG ACGACCACAC CAATCGTTAC ATTGACGATG AGCCAGATGA TGGTGCCCAA 
CATTACATTA TAGTTGTGGG CTTTAACAAT CCTCAGCTAT TAGCAATTAT GGCTGAGCTT 
GGCATTCCTA TAACCAGCGT GATTAAAATA TCTTCAGAGA ATTATGAACC TCTGCAGACA 
CACCTGGCAG CAGTTAACCA GCAGCAGGAA GTTCTTCTTC AGTCAGAAGA TCTAGAAGCA 
GAAAAATTGA AGAAAGAAAA TGCCATAAAA GAGCTTAAAA CTTTCTGGAA GTACTTGGAA 
CCAGTCCTGA ATAATGAGAA ACCTGAAACA AATCTCTTTG ATGTTGCTCG ACTTGAGTAC 
ATGGTCAAAG CAGCTGATTT TCCTTCTGAC TGGTCAGATG GTGAGATGAT GCTGAAATTG 
GGCACTGATA TTTTTGAAAA TATTGCCTGC TTGATGTATG ACATCCTGGA TTGGAAAAGG 
CAGCACCAGC ACTATTTGGA AAGCATGCAG CTTATTAATG TTCCACAAGT GGTTAATGAG 
AAACCTGTAT TAGAAGCCAT GCCAACTTCA GAGGCTCCAC AACCTGCTGT ACCAGCTCCT 
GGAAAGAAGA AAGCACAGTA TGAAGAACCG CAAGCTCCAC CACCAGTGAC TTCAGTCATC 
ACAACTGAAG TAGACATGAG ATATTACAAT TATTTGCTGA ATCCAATTCG AGAGGAATTC 
ATTTCTGTGC CCCTGATACT GCATTGTATG CTGGAACAGG TTGTTGCAAC TGAAGAAGAT 
CTCGTCCCAC CCAGTCTGCG GGAGCCATCC CCCAGAGCAG ACGGGCTAGA CCACAGAATC 
GCAGCTCACA TTGTGTCCCT TCTGCCCTCA CTCTGTCTCT CAGAGAGGGA GAAAAAGAAT 
CTTCATGACA TATTTTTATC TGAAGAAGAA AATGAAAGCA AAGCAGTGCC CAAAGGCCCC 
CTCCTACTGA ACTATCATGA TGCACACGCC CACAAGAAGT ACGCACTACA GGACCAAAAG 
AATTTTGATC CAGTTCAAAT TGAGCAGGAG ATGCAGTCCA AGTTGCCACT GTGGGAATTT 
CTTCAATTCC CTCTACCCCC ACCATGGAAC AACACTAAAC GTCTAGCTAC AATTCATGAG 
CTTATGCACT TTTGTACGAG TGATGTCTTG AGCTGGAATG AAGTAGAACG AGCCTTCAAG 
GTGTTTACTT TTGAGAGCCT GAAGCTCTCT GAGGTTGATG AAAAAGGGAA ACTGAAACCT 
TCTGGGATGA TGTGTGGGTC AGATTCTGAA ATGTTCAACA TACCGTGGGA CAACCCTGCC 
AGATTTGCTA AACAGATAAG GCAGCAATAT GTCATGAAAA TGAATACTCA AGAGGCCAAG 
CAGAAAGCAG ATATTAAAAT CAAAGACAGA ACACTATTTG TGGATCAGAA TTTGTCAATG 
TCTGTGCAAG ATAATGAAAG CAACCGAGAA CCTTCAGATC CTAGTCAGTG TGATGCTAAC 
AATATGAAGC ATTCTGACTT GAATAATCTC AAACTCTCAG TCCCTGATAA TAGACAGCTG 
TTAGAGCAGG AGAGCATCAT GAAGGCTCAG CCCCAACATG AGTCTCTGGA GCAGACCACA 
AACAATGAGA TCAAAGATGA TGCAGTCACA AAGGCTGATT CTCATGAAAA GAAACCCAAG 
AAGATGATGG TGGAAGCAGA TTTAGAGGAC ATAAAGAAAA CACAGCAGCG CAGTCTAATG 
GACTGGAGTT TTACTGAACA TTTTAAACCG AAAGTACTGC TTCAGGTCCT TCAAGAAGCC 
CATAAGCAAT ATAGGTGTGT TGATTCTTAC TACCACACCC AAGACAACTC TTTACTTTTA 
GTCTTTCACA ATCCAATGAA TAGACAACGT TTGCATTGTG AATATTGGAA CATTGCTCTC 
CACTCCAATG TTGGATTCAG GAATTATTTG GAACTTGTTG CAAAATCTAT TCAAGATTGG 
ATTACAAAAG AAGAAGCTAT ATATCAGGAA TCTAAAATGA ATGAGAAAAT CATCAGGACC 
AGAGCTGAGC TGGAATTGAA ATCTTCTGCT AATGCCAAAC TTACTTCTGC TAGCAAAATT 
TTTTCCATTA AAGAATCTAA AAGTAACAAA GGAATCAGCA AAACAGAGAT ATCAGATCAA 
GAAAAAGAAA AAGAGAAGGA AAAGATTCCT TTCATTTTAG AAGGCTCTCT CAAGGCATGG 
AAAGAAGAGC AACATCGATT AGCAGAAGAG GAGCGCTTAA GGGAAGAAAA GAAAGCAGAG 
AAGAAGGGTA AAGAAGCTGG TAAAAAGAAA GGCAAGGATA ACGCAGAGAA AGAGGATAGT 
AGGTCTTTGA AGAAAAAATC ACCTTACAAG GAGAAATCTA AAGAAGAACA AGTCAAGATC 
CAAGAAGTAA CAGAAGAGTC CCCCCACCAA CCAGAACCTA AGATAACTTA CCCGTTTCAC 
GGATACAATA TGGGAAATAT ACCCACTCAA ATCTCAGGGT CAAATTACTA CCTGTATCCT 
TCTGATGGGG GGCAGATTGA AGTGGAAAAG ACAATGTTTG AAAAAGGCCC AACTTTTATC 
AAAGTGAGAG TGGTAAAGGA CAACCACAAT TTTATGATTC ATTTAAATGA CCCTAAGGAA 
ATTGTGAAAA AGGAAGAGAA AGGGGATTAT TATTTAGAAG AGGAAGAAGA AGGAGATGAG 
GAACAAAGTC TTGAAACGGA AGTATCAGAT GCAAAGAATA AAGCTTTCAG CAAGTTTGGA 
TCTTTTTCTG CCACCTTAGA AAATGGAATC TGCCTCTCGA TAAGTTACTA TGGATCAAAT 
GGAATGGCAC CAGAAGATAA GGATCCTGAT TTAGAAACAA TATTGAATAT CCCTTCAGCA 
CTCACTCCAA CAGTGGTTCC TGTTATAGTG ACCGTTCCTC AAAGCAAAGC TAAAGGGAAA 
ATAAAAGGCA AAGAAAAACC CAAAGAATCC CTTAAAGAAG AAGAACACCC AAAAGAAGAA 
GAGAAAAAGG AAGAAGAAGT AGAACCAGAA CCTGTTTTAC AAGAGACTTT GGATGTTCCC 
ACCTTCCAGA GCCTAAATGT GTCTTGCCCC AGTGGGCTCC TGTTGACTTT CATTGGACAA 
GAATCTACAG GTCAATATGT TATAGATGAG GAACCCACCT GGGACATCAT GGTCCGTCAG 
AGCTACCCCC AGAGGGTGAA GCACTATGAG TTCTATAAAA CGGTGATGCC ACCCGCAGAG 
CAGGAGGCTT CAAGGGTTAT CACCAGTCAA GGCACTGTTG TCAAATATAT GTTGGATGGA 
TCCACACAGA TTCTCTTTGC AGATGGTGCT GTGAGCAGGA GTCCCAATTC AGGTCTTATT 
TGTCCTCCTT CTGAAATGCC AGCAACGCCT CACAGTGGAG ATTTGATGGA CTCTATTTCT 
CAGCAGAAAT CAGAAACGAT ACCATCTGAG ATTACCAACA CAAAGAAAGG AAAAAGTCAC 
AAAAGTCAGT CATCAATGGC CCATAAGGGT GAAATCCATG ACCCTCCTCC AGAGGCAGTT 
CAAACTGTAA CTCCTGTGGA GGTTCACATA GGCACCTGGT TTACAACCAC ACCTGAAGGA 
AATCGGATCG GCACCAAAGG ATTAGAAAGA ATAGCAGACT TGACCCCATT GTTATCCTTT 
CAGGCCACAG ATCCTGTCAA TGGAACGGTT ATGACAACTC GAGAAGACAA AGTTGTCATA 
GTTGAAAGGA AAGATGGTAC TCGGATAGTG GATCATGCTG ATGGTACCAG AATCACAACC 
TTTTATCAAG TTTATGAAGA TCAAATTATT CTGCCAGATG ATCAAGAAAC AACCGAGGGT 
CCTCGGACTG TCACCAGGCA GGTGAAGTGT ATGCGGGTAG AAAGCTCACG CTATGCCACT 
GTTATCGCCA ACTGTGAGGA CAGTAGCTGC TGTGCCACCT TTGGAGATGG AACAACTATT 
ATTGCAAAGC CACAGGGAAC ATACCAGGTG TTACCTCCAA ACACAGGCTC TCTTTATATT 
GACAAGGATT GTTCAGCTGT GTACTGCCAT GAGTCAAGCA GTAATATATA CTATCCTTTT 
CAAAAGCGTG AGCAGCTGCG AGCTGGCAGG TACATCATGA GGCATACTTC AGAGGTTATC 
TGTGAGGTTC TGGATCCTGA GGGAAACACT TTTCAGGTCA TGGCTGATGG TAGCATATCA 
ACTATATTAC CTGAAAAAAA ATTGGAAGAT GATTTAAATG AGAAAACTGA GGGCTATGAT 
AGTCTGTCCT CTATGCACCT TGAAAAGAAT CATCAGCAAA TCTATGGTGA ACATGTCCCC 
AGGTTTTTTG TTATGTATGC TGATGGATCA GGAATGGAAC TTCTTCGAGA CAGTGACATA 
GAAGAATATC TATCTTTGGC ATATAAAGAA TCAAATACTG TTGTTCTCCA AGAGCCAGTG 
CAGGAACAGC CAGGCACCCT AACCATCACA GTCCTTCGCC CTTTCCATGA AGCATCACCA 
TGGCAAGTAA AAAAGGAAGA TACAATTGTC CCTCCTAATC TCCGGTCAAG GTCATGGGAA 
ACATTTCCCT CAGTTGAGAA AAAAACTCCA GGACCTCCGT TTGGTACTCA GATTTGGAAA 
GGCCTTTGCA TTGAGTCCAA ACAGCTAGTG AGTGCCCCGG GTGCCATACT CAAGAGCCCC 
AGTGTGCTAC AGATGCGCCA ATTCATTCAG CATGAGGTCA TAAAGAATGA GGTGAAACTG 
AGGCTGCAGG TTTCCCTTAA GGATTACATA AACTATATTC TAAAGAAAGA AGATGAGCTG 
CAGGAAATGA TGGTTAAAGA TTCCAGAACT GAGGAGGAGA GAGGCAATGC TGCTGATCTC 
CTCAAGCTGG TTATGTCTTT CCCTAAAATG GAGGAAACTA CAAAAAGTCA TGTTACTGAA 
GTTGCAGCTC ACCTAACTGA TTTATTCAAG CAGTCTTTGG CTACGCCTCC AAAATGCCCA 
CCAGACACAT TTGGTAAAGA TTTCTTTGAA AAGACATGGA GACACACAGC ATCCTCAAAA 
CGCTGGAAAG AAAAGATAGA CAAAACGAGG AAGGAAATTG AGACAACACA GAATTACCTA 
ATGGATATTA AGAACCGCAT AATACCACCC TTTTTTAAAT CTGAATTGAA CCAGTTATAT 
CAGTCTCAGT ATAATCACCT GGACAGTCTT TCCAAAAAAC TGCCTTCTTT TACAAAGAAA 
AATGAAGATG CAAACGAAAC AGCTGTTCAA GATACATCTG ATCTTAATCT AGATTTCAAG 
CCACATAAGG TTTCAGAACA GAAATCCTCA AGTGTGCCTA GTCTTCCAAA ACCAGAGATT 
TCTGCAGATA AGAAGGATTT CACTGCTCAG AACCAAACTG AAAATTTAAC AAAATCTCCT 
GAAGAAGCAG AATCTTATGA GCCCGTGAAA ATTCCAACCC AGTCCTTGCT GCAGGATGTT 
GCGGGACAAA CAAGAAAAGA AAAAGTGAAG TTGCCTCATT ATTTGCTGAG TTCCAAGCCT 
AAGTCTCAAC CTCTTGCAAA GGTGCAAGAT TCTGTTGGAG GAAAAGTGAA CACATCCTCT 
GTTGCATCTG CTGCCATTAA TAATGCAAAG TCATCCCTTT TTGGGTTCCA TCTTCTCCCA 
TCATCAGTCA AGTTTGGAGT GCTTAAGGAA GGACATACCT ATGCCACAGT TGTAAAGCTC 
AAGAATGTTG GAGTGGACTT CTGCAGGTTT AAAGTAAAGC AGCCCCCACC CAGCACAGGA 
CTGAAAGTGA CTTACAAACC TGGACCTGTG GCAGCTGGTA TGCAGACAGA ACTGAATATA 
GAGTTATTTG CCACAGCTGT TGGAGAGGAT GGGGCCAAGG GATCAGCACA CATCTCTCAC 
AATATCGAGA TTATGACAGA GCATGAGGTT CTGTTCCTAC CTGTGGAAGC AACTGTTTTA 
ACAAGCAGCA ATTATGATAA ACGACCAAAA GACTTTCCCC AGGGAAAAGA AAATCCAATG 
GTCCAGAGAA CTTCTACAAT TTATTCCTCC ACACTTGGAG TCTTCATGTC TCGTAAAGTT 
TCTCCACATT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPAG17 ( XM_006710431.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_006710494.1 Homo sapiens sperm associated antigen 17 (SPAG17), transcript variant X13, mRNA.

Price & Availability↑

CloneID	OHu33136
Clone ID Related Accession (Same CDS sequence)	XM_006710431.3
Accession Version	XM_006710431.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3459bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	sperm-associated antigen 17 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006710431.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTCATT TAAATGACCC TAAGGAAATT GTGAAAAAGG AAGAGAAAGG GGATTATTAT 
TTAGAAGAGG AAGAAGAAGG AGATGAGGAA CAAAGTCTTG AAACGGAAGT ATCAGATGCA 
AAGAATAAAG CTTTCAGCAA GTTTGGATCT TTTTCTGCCA CCTTAGAAAA TGGAATCTGC 
CTCTCGATAA GTTACTATGG ATCAAATGGA ATGGCACCAG AAGATAAGGA TCCTGATTTA 
GAAACAATAT TGAATATCCC TTCAGCACTC ACTCCAACAG TGGTTCCTGT TATAGTGACC 
GTTCCTCAAA GCAAAGCTAA AGGGAAAATA AAAGGCAAAG AAAAACCCAA AGAATCCCTT 
AAAGAAGAAG AACACCCAAA AGAAGAAGAG AAAAAGGAAG AAGAAGTAGA ACCAGAACCT 
GTTTTACAAG AGACTTTGGA TGTTCCCACC TTCCAGAGCC TAAATGTGTC TTGCCCCAGT 
GGGCTCCTGT TGACTTTCAT TGGACAAGAA TCTACAGGTC AATATGTTAT AGATGAGGAA 
CCCACCTGGG ACATCATGGT CCGTCAGAGC TACCCCCAGA GGGTGAAGCA CTATGAGTTC 
TATAAAACGG TGATGCCACC CGCAGAGCAG GAGGCTTCAA GGGTTATCAC CAGTCAAGGC 
ACTGTTGTCA AATATATGTT GGATGGATCC ACACAGATTC TCTTTGCAGA TGGTGCTGTG 
AGCAGGAGTC CCAATTCAGG TCTTATTTGT CCTCCTTCTG AAATGCCAGC AACGCCTCAC 
AGTGGAGATT TGATGGACTC TATTTCTCAG CAGAAATCAG AAACGATACC ATCTGAGATT 
ACCAACACAA AGAAAGGAAA AAGTCACAAA AGTCAGTCAT CAATGGCCCA TAAGGGTGAA 
ATCCATGACC CTCCTCCAGA GGCAGTTCAA ACTGTAACTC CTGTGGAGGT TCACATAGGC 
ACCTGGTTTA CAACCACACC TGAAGGAAAT CGGATCGGCA CCAAAGGATT AGAAAGAATA 
GCAGACTTGA CCCCATTGTT ATCCTTTCAG GCCACAGATC CTGTCAATGG AACGGTTATG 
ACAACTCGAG AAGACAAAGT TGTCATAGTT GAAAGGAAAG ATGGTACTCG GATAGTGGAT 
CATGCTGATG GTACCAGAAT CACAACCTTT TATCAAGTTT ATGAAGATCA AATTATTCTG 
CCAGATGATC AAGAAACAAC CGAGGGTCCT CGGACTGTCA CCAGGCAGGT GAAGTGTATG 
CGGGTAGAAA GCTCACGCTA TGCCACTGTT ATCGCCAACT GTGAGGACAG TAGCTGCTGT 
GCCACCTTTG GAGATGGAAC AACTATTATT GCAAAGCCAC AGGGAACATA CCAGGTGTTA 
CCTCCAAACA CAGGCTCTCT TTATATTGAC AAGGATTGTT CAGCTGTGTA CTGCCATGAG 
TCAAGCAGTA ATATATACTA TCCTTTTCAA AAGCGTGAGC AGCTGCGAGC TGGCAGGTAC 
ATCATGAGGC ATACTTCAGA GGTTATCTGT GAGGTTCTGG ATCCTGAGGG AAACACTTTT 
CAGGTCATGG CTGATGGTAG CATATCAACT ATATTACCTG AAAAAAAATT GGAAGATGAT 
TTAAATGAGA AAACTGAGGG CTATGATAGT CTGTCCTCTA TGCACCTTGA AAAGAATCAT 
CAGCAAATCT ATGGTGAACA TGTCCCCAGG TTTTTTGTTA TGTATGCTGA TGGATCAGGA 
ATGGAACTTC TTCGAGACAG TGACATAGAA GAATATCTAT CTTTGGCATA TAAAGAATCA 
AATACTGTTG TTCTCCAAGA GCCAGTGCAG GAACAGCCAG GCACCCTAAC CATCACAGTC 
CTTCGCCCTT TCCATGAAGC ATCACCATGG CAAGTAAAAA AGGAAGATAC AATTGTCCCT 
CCTAATCTCC GGTCAAGGTC ATGGGAAACA TTTCCCTCAG TTGAGAAAAA AACTCCAGGA 
CCTCCGTTTG GTACTCAGAT TTGGAAAGGC CTTTGCATTG AGTCCAAACA GCTAGTGAGT 
GCCCCGGGTG CCATACTCAA GAGCCCCAGT GTGCTACAGA TGCGCCAATT CATTCAGCAT 
GAGGTCATAA AGAATGAGGT GAAACTGAGG CTGCAGGTTT CCCTTAAGGA TTACATAAAC 
TATATTCTAA AGAAAGAAGA TGAGCTGCAG GAAATGATGG TTAAAGATTC CAGAACTGAG 
GAGGAGAGAG GCAATGCTGC TGATCTCCTC AAGCTGGTTA TGTCTTTCCC TAAAATGGAG 
GAAACTACAA AAAGTCATGT TACTGAAGTT GCAGCTCACC TAACTGATTT ATTCAAGCAG 
TCTTTGGCTA CGCCTCCAAA ATGCCCACCA GACACATTTG GTAAAGATTT CTTTGAAAAG 
ACATGGAGAC ACACAGCATC CTCAAAACGC TGGAAAGAAA AGATAGACAA AACGAGGAAG 
GAAATTGAGA CAACACAGAA TTACCTAATG GATATTAAGA ACCGCATAAT ACCACCCTTT 
TTTAAATCTG AATTGAACCA GTTATATCAG TCTCAGTATA ATCACCTGGA CAGTCTTTCC 
AAAAAACTGC CTTCTTTTAC AAAGAAAAAT GAAGATGCAA ACGAAACAGC TGTTCAAGAT 
ACATCTGATC TTAATCTAGA TTTCAAGCCA CATAAGGTTT CAGAACAGAA ATCCTCAAGT 
GTGCCTAGTC TTCCAAAACC AGAGATTTCT GCAGATAAGA AGGATTTCAC TGCTCAGAAC 
CAAACTGAAA ATTTAACAAA ATCTCCTGAA GAAGCAGAAT CTTATGAGCC CGTGAAAATT 
CCAACCCAGT CCTTGCTGCA GGATGTTGCG GGACAAACAA GAAAAGAAAA AGTGAAGTTG 
CCTCATTATT TGCTGAGTTC CAAGCCTAAG TCTCAACCTC TTGCAAAGGT GCAAGATTCT 
GTTGGAGGAA AAGTGAACAC ATCCTCTGTT GCATCTGCTG CCATTAATAA TGCAAAGTCA 
TCCCTTTTTG GGTTCCATCT TCTCCCATCA TCAGTCAAGT TTGGAGTGCT TAAGGAAGGA 
CATACCTATG CCACAGTTGT AAAGCTCAAG AATGTTGGAG TGGACTTCTG CAGGTTTAAA 
GTAAAGCAGC CCCCACCCAG CACAGGACTG AAAGTGACTT ACAAACCTGG ACCTGTGGCA 
GCTGGTATGC AGACAGAACT GAATATAGAG TTATTTGCCA CAGCTGTTGG AGAGGATGGG 
GCCAAGGGAT CAGCACACAT CTCTCACAAT ATCGAGATTA TGACAGAGCA TGAGGTTCTG 
TTCCTACCTG TGGAAGCAAC TGTTTTAACA AGCAGCAATT ATGATAAACG ACCAAAAGAC 
TTTCCCCAGG GAAAAGAAAA TCCAATGGTC CAGAGAACTT CTACAATTTA TTCCTCCACA 
CTTGGAGTCT TCATGTCTCG TAAAGTTTCT CCACATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006710494.1
CDS	412..3870
Translation	MIHLNDPKEIVKKEEKGDYYLEEEEEGDEEQSLETEVSDAKNKAFSKFGSFSATLENGICLSISYYGSNGMAPEDKDPDLETILNIPSALTPTVVPVIVTVPQSKAKGKIKGKEKPKESLKEEEHPKEEEKKEEEVEPEPVLQETLDVPTFQSLNVSCPSGLLLTFIGQESTGQYVIDEEPTWDIMVRQSYPQRVKHYEFYKTVMPPAEQEASRVITSQGTVVKYMLDGSTQILFADGAVSRSPNSGLICPPSEMPATPHSGDLMDSISQQKSETIPSEITNTKKGKSHKSQSSMAHKGEIHDPPPEAVQTVTPVEVHIGTWFTTTPEGNRIGTKGLERIADLTPLLSFQATDPVNGTVMTTREDKVVIVERKDGTRIVDHADGTRITTFYQVYEDQIILPDDQETTEGPRTVTRQVKCMRVESSRYATVIANCEDSSCCATFGDGTTIIAKPQGTYQVLPPNTGSLYIDKDCSAVYCHESSSNIYYPFQKREQLRAGRYIMRHTSEVICEVLDPEGNTFQVMADGSISTILPEKKLEDDLNEKTEGYDSLSSMHLEKNHQQIYGEHVPRFFVMYADGSGMELLRDSDIEEYLSLAYKESNTVVLQEPVQEQPGTLTITVLRPFHEASPWQVKKEDTIVPPNLRSRSWETFPSVEKKTPGPPFGTQIWKGLCIESKQLVSAPGAILKSPSVLQMRQFIQHEVIKNEVKLRLQVSLKDYINYILKKEDELQEMMVKDSRTEEERGNAADLLKLVMSFPKMEETTKSHVTEVAAHLTDLFKQSLATPPKCPPDTFGKDFFEKTWRHTASSKRWKEKIDKTRKEIETTQNYLMDIKNRIIPPFFKSELNQLYQSQYNHLDSLSKKLPSFTKKNEDANETAVQDTSDLNLDFKPHKVSEQKSSSVPSLPKPEISADKKDFTAQNQTENLTKSPEEAESYEPVKIPTQSLLQDVAGQTRKEKVKLPHYLLSSKPKSQPLAKVQDSVGGKVNTSSVASAAINNAKSSLFGFHLLPSSVKFGVLKEGHTYATVVKLKNVGVDFCRFKVKQPPPSTGLKVTYKPGPVAAGMQTELNIELFATAVGEDGAKGSAHISHNIEIMTEHEVLFLPVEATVLTSSNYDKRPKDFPQGKENPMVQRTSTIYSSTLGVFMSRKVSPH

Target ORF information:

RefSeq Version	XM_006710431.3
Organism	Homo sapiens(human)
Definition	Homo sapiens sperm associated antigen 17 (SPAG17), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006710431.3

ORF Insert Sequence:

ATGATTCATT TAAATGACCC TAAGGAAATT GTGAAAAAGG AAGAGAAAGG GGATTATTAT 
TTAGAAGAGG AAGAAGAAGG AGATGAGGAA CAAAGTCTTG AAACGGAAGT ATCAGATGCA 
AAGAATAAAG CTTTCAGCAA GTTTGGATCT TTTTCTGCCA CCTTAGAAAA TGGAATCTGC 
CTCTCGATAA GTTACTATGG ATCAAATGGA ATGGCACCAG AAGATAAGGA TCCTGATTTA 
GAAACAATAT TGAATATCCC TTCAGCACTC ACTCCAACAG TGGTTCCTGT TATAGTGACC 
GTTCCTCAAA GCAAAGCTAA AGGGAAAATA AAAGGCAAAG AAAAACCCAA AGAATCCCTT 
AAAGAAGAAG AACACCCAAA AGAAGAAGAG AAAAAGGAAG AAGAAGTAGA ACCAGAACCT 
GTTTTACAAG AGACTTTGGA TGTTCCCACC TTCCAGAGCC TAAATGTGTC TTGCCCCAGT 
GGGCTCCTGT TGACTTTCAT TGGACAAGAA TCTACAGGTC AATATGTTAT AGATGAGGAA 
CCCACCTGGG ACATCATGGT CCGTCAGAGC TACCCCCAGA GGGTGAAGCA CTATGAGTTC 
TATAAAACGG TGATGCCACC CGCAGAGCAG GAGGCTTCAA GGGTTATCAC CAGTCAAGGC 
ACTGTTGTCA AATATATGTT GGATGGATCC ACACAGATTC TCTTTGCAGA TGGTGCTGTG 
AGCAGGAGTC CCAATTCAGG TCTTATTTGT CCTCCTTCTG AAATGCCAGC AACGCCTCAC 
AGTGGAGATT TGATGGACTC TATTTCTCAG CAGAAATCAG AAACGATACC ATCTGAGATT 
ACCAACACAA AGAAAGGAAA AAGTCACAAA AGTCAGTCAT CAATGGCCCA TAAGGGTGAA 
ATCCATGACC CTCCTCCAGA GGCAGTTCAA ACTGTAACTC CTGTGGAGGT TCACATAGGC 
ACCTGGTTTA CAACCACACC TGAAGGAAAT CGGATCGGCA CCAAAGGATT AGAAAGAATA 
GCAGACTTGA CCCCATTGTT ATCCTTTCAG GCCACAGATC CTGTCAATGG AACGGTTATG 
ACAACTCGAG AAGACAAAGT TGTCATAGTT GAAAGGAAAG ATGGTACTCG GATAGTGGAT 
CATGCTGATG GTACCAGAAT CACAACCTTT TATCAAGTTT ATGAAGATCA AATTATTCTG 
CCAGATGATC AAGAAACAAC CGAGGGTCCT CGGACTGTCA CCAGGCAGGT GAAGTGTATG 
CGGGTAGAAA GCTCACGCTA TGCCACTGTT ATCGCCAACT GTGAGGACAG TAGCTGCTGT 
GCCACCTTTG GAGATGGAAC AACTATTATT GCAAAGCCAC AGGGAACATA CCAGGTGTTA 
CCTCCAAACA CAGGCTCTCT TTATATTGAC AAGGATTGTT CAGCTGTGTA CTGCCATGAG 
TCAAGCAGTA ATATATACTA TCCTTTTCAA AAGCGTGAGC AGCTGCGAGC TGGCAGGTAC 
ATCATGAGGC ATACTTCAGA GGTTATCTGT GAGGTTCTGG ATCCTGAGGG AAACACTTTT 
CAGGTCATGG CTGATGGTAG CATATCAACT ATATTACCTG AAAAAAAATT GGAAGATGAT 
TTAAATGAGA AAACTGAGGG CTATGATAGT CTGTCCTCTA TGCACCTTGA AAAGAATCAT 
CAGCAAATCT ATGGTGAACA TGTCCCCAGG TTTTTTGTTA TGTATGCTGA TGGATCAGGA 
ATGGAACTTC TTCGAGACAG TGACATAGAA GAATATCTAT CTTTGGCATA TAAAGAATCA 
AATACTGTTG TTCTCCAAGA GCCAGTGCAG GAACAGCCAG GCACCCTAAC CATCACAGTC 
CTTCGCCCTT TCCATGAAGC ATCACCATGG CAAGTAAAAA AGGAAGATAC AATTGTCCCT 
CCTAATCTCC GGTCAAGGTC ATGGGAAACA TTTCCCTCAG TTGAGAAAAA AACTCCAGGA 
CCTCCGTTTG GTACTCAGAT TTGGAAAGGC CTTTGCATTG AGTCCAAACA GCTAGTGAGT 
GCCCCGGGTG CCATACTCAA GAGCCCCAGT GTGCTACAGA TGCGCCAATT CATTCAGCAT 
GAGGTCATAA AGAATGAGGT GAAACTGAGG CTGCAGGTTT CCCTTAAGGA TTACATAAAC 
TATATTCTAA AGAAAGAAGA TGAGCTGCAG GAAATGATGG TTAAAGATTC CAGAACTGAG 
GAGGAGAGAG GCAATGCTGC TGATCTCCTC AAGCTGGTTA TGTCTTTCCC TAAAATGGAG 
GAAACTACAA AAAGTCATGT TACTGAAGTT GCAGCTCACC TAACTGATTT ATTCAAGCAG 
TCTTTGGCTA CGCCTCCAAA ATGCCCACCA GACACATTTG GTAAAGATTT CTTTGAAAAG 
ACATGGAGAC ACACAGCATC CTCAAAACGC TGGAAAGAAA AGATAGACAA AACGAGGAAG 
GAAATTGAGA CAACACAGAA TTACCTAATG GATATTAAGA ACCGCATAAT ACCACCCTTT 
TTTAAATCTG AATTGAACCA GTTATATCAG TCTCAGTATA ATCACCTGGA CAGTCTTTCC 
AAAAAACTGC CTTCTTTTAC AAAGAAAAAT GAAGATGCAA ACGAAACAGC TGTTCAAGAT 
ACATCTGATC TTAATCTAGA TTTCAAGCCA CATAAGGTTT CAGAACAGAA ATCCTCAAGT 
GTGCCTAGTC TTCCAAAACC AGAGATTTCT GCAGATAAGA AGGATTTCAC TGCTCAGAAC 
CAAACTGAAA ATTTAACAAA ATCTCCTGAA GAAGCAGAAT CTTATGAGCC CGTGAAAATT 
CCAACCCAGT CCTTGCTGCA GGATGTTGCG GGACAAACAA GAAAAGAAAA AGTGAAGTTG 
CCTCATTATT TGCTGAGTTC CAAGCCTAAG TCTCAACCTC TTGCAAAGGT GCAAGATTCT 
GTTGGAGGAA AAGTGAACAC ATCCTCTGTT GCATCTGCTG CCATTAATAA TGCAAAGTCA 
TCCCTTTTTG GGTTCCATCT TCTCCCATCA TCAGTCAAGT TTGGAGTGCT TAAGGAAGGA 
CATACCTATG CCACAGTTGT AAAGCTCAAG AATGTTGGAG TGGACTTCTG CAGGTTTAAA 
GTAAAGCAGC CCCCACCCAG CACAGGACTG AAAGTGACTT ACAAACCTGG ACCTGTGGCA 
GCTGGTATGC AGACAGAACT GAATATAGAG TTATTTGCCA CAGCTGTTGG AGAGGATGGG 
GCCAAGGGAT CAGCACACAT CTCTCACAAT ATCGAGATTA TGACAGAGCA TGAGGTTCTG 
TTCCTACCTG TGGAAGCAAC TGTTTTAACA AGCAGCAATT ATGATAAACG ACCAAAAGAC 
TTTCCCCAGG GAAAAGAAAA TCCAATGGTC CAGAGAACTT CTACAATTTA TTCCTCCACA 
CTTGGAGTCT TCATGTCTCG TAAAGTTTCT CCACATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPAG17 ( XM_011540939.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011539241.1 Homo sapiens sperm associated antigen 17 (SPAG17), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OHu33133
Clone ID Related Accession (Same CDS sequence)	XM_011540939.2 , XM_006710428.3
Accession Version	XM_011540939.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4491bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	sperm-associated antigen 17 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011540939.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTCGTA TTGGTCCATG GAGTCCAGAG CAGACCACAA ACAATGAGAT CAAAGATGAT 
GCAGTCACAA AGGCTGATTC TCATGAAAAG AAACCCAAGA AGATGATGGT GGAAGCAGAT 
TTAGAGGACA TAAAGAAAAC ACAGCAGCGC AGTCTAATGG ACTGGAGTTT TACTGAACAT 
TTTAAACCGA AAGTACTGCT TCAGGTCCTT CAAGAAGCCC ATAAGCAATA TAGGTGTGTT 
GATTCTTACT ACCACACCCA AGACAACTCT TTACTTTTAG TCTTTCACAA TCCAATGAAT 
AGACAACGTT TGCATTGTGA ATATTGGAAC ATTGCTCTCC ACTCCAATGT TGGATTCAGG 
AATTATTTGG AACTTGTTGC AAAATCTATT CAAGATTGGA TTACAAAAGA AGAAGCTATA 
TATCAGGAAT CTAAAATGAA TGAGAAAATC ATCAGGACCA GAGCTGAGCT GGAATTGAAA 
TCTTCTGCTA ATGCCAAACT TACTTCTGCT AGCAAAATTT TTTCCATTAA AGAATCTAAA 
AGTAACAAAG GAATCAGCAA AACAGAGATA TCAGATCAAG AAAAAGAAAA AGAGAAGGAA 
AAGATTCCTT TCATTTTAGA AGGCTCTCTC AAGGCATGGA AAGAAGAGCA ACATCGATTA 
GCAGAAGAGG AGCGCTTAAG GGAAGAAAAG AAAGCAGAGA AGAAGGGTAA AGAAGCTGGT 
AAAAAGAAAG GCAAGGATAA CGCAGAGAAA GAGGATAGTA GGTCTTTGAA GAAAAAATCA 
CCTTACAAGG AGAAATCTAA AGAAGAACAA GTCAAGATCC AAGAAGTAAC AGAAGAGTCC 
CCCCACCAAC CAGAACCTAA GATAACTTAC CCGTTTCACG GATACAATAT GGGAAATATA 
CCCACTCAAA TCTCAGGGTC AAATTACTAC CTGTATCCTT CTGATGGGGG GCAGATTGAA 
GTGGAAAAGA CAATGTTTGA AAAAGGCCCA ACTTTTATCA AAGTGAGAGT GGTAAAGGAC 
AACCACAATT TTATGATTCA TTTAAATGAC CCTAAGGAAA TTGTGAAAAA GGAAGAGAAA 
GGGGATTATT ATTTAGAAGA GGAAGAAGAA GGAGATGAGG AACAAAGTCT TGAAACGGAA 
GTATCAGATG CAAAGAATAA AGCTTTCAGC AAGTTTGGAT CTTTTTCTGC CACCTTAGAA 
AATGGAATCT GCCTCTCGAT AAGTTACTAT GGATCAAATG GAATGGCACC AGAAGATAAG 
GATCCTGATT TAGAAACAAT ATTGAATATC CCTTCAGCAC TCACTCCAAC AGTGGTTCCT 
GTTATAGTGA CCGTTCCTCA AAGCAAAGCT AAAGGGAAAA TAAAAGGCAA AGAAAAACCC 
AAAGAATCCC TTAAAGAAGA AGAACACCCA AAAGAAGAAG AGAAAAAGGA AGAAGAAGTA 
GAACCAGAAC CTGTTTTACA AGAGACTTTG GATGTTCCCA CCTTCCAGAG CCTAAATGTG 
TCTTGCCCCA GTGGGCTCCT GTTGACTTTC ATTGGACAAG AATCTACAGG TCAATATGTT 
ATAGATGAGG AACCCACCTG GGACATCATG GTCCGTCAGA GCTACCCCCA GAGGGTGAAG 
CACTATGAGT TCTATAAAAC GGTGATGCCA CCCGCAGAGC AGGAGGCTTC AAGGGTTATC 
ACCAGTCAAG GCACTGTTGT CAAATATATG TTGGATGGAT CCACACAGAT TCTCTTTGCA 
GATGGTGCTG TGAGCAGGAG TCCCAATTCA GGTCTTATTT GTCCTCCTTC TGAAATGCCA 
GCAACGCCTC ACAGTGGAGA TTTGATGGAC TCTATTTCTC AGCAGAAATC AGAAACGATA 
CCATCTGAGA TTACCAACAC AAAGAAAGGA AAAAGTCACA AAAGTCAGTC ATCAATGGCC 
CATAAGGGTG AAATCCATGA CCCTCCTCCA GAGGCAGTTC AAACTGTAAC TCCTGTGGAG 
GTTCACATAG GCACCTGGTT TACAACCACA CCTGAAGGAA ATCGGATCGG CACCAAAGGA 
TTAGAAAGAA TAGCAGACTT GACCCCATTG TTATCCTTTC AGGCCACAGA TCCTGTCAAT 
GGAACGGTTA TGACAACTCG AGAAGACAAA GTTGTCATAG TTGAAAGGAA AGATGGTACT 
CGGATAGTGG ATCATGCTGA TGGTACCAGA ATCACAACCT TTTATCAAGT TTATGAAGAT 
CAAATTATTC TGCCAGATGA TCAAGAAACA ACCGAGGGTC CTCGGACTGT CACCAGGCAG 
GTGAAGTGTA TGCGGGTAGA AAGCTCACGC TATGCCACTG TTATCGCCAA CTGTGAGGAC 
AGTAGCTGCT GTGCCACCTT TGGAGATGGA ACAACTATTA TTGCAAAGCC ACAGGGAACA 
TACCAGGTGT TACCTCCAAA CACAGGCTCT CTTTATATTG ACAAGGATTG TTCAGCTGTG 
TACTGCCATG AGTCAAGCAG TAATATATAC TATCCTTTTC AAAAGCGTGA GCAGCTGCGA 
GCTGGCAGGT ACATCATGAG GCATACTTCA GAGGTTATCT GTGAGGTTCT GGATCCTGAG 
GGAAACACTT TTCAGGTCAT GGCTGATGGT AGCATATCAA CTATATTACC TGAAAAAAAA 
TTGGAAGATG ATTTAAATGA GAAAACTGAG GGCTATGATA GTCTGTCCTC TATGCACCTT 
GAAAAGAATC ATCAGCAAAT CTATGGTGAA CATGTCCCCA GGTTTTTTGT TATGTATGCT 
GATGGATCAG GAATGGAACT TCTTCGAGAC AGTGACATAG AAGAATATCT ATCTTTGGCA 
TATAAAGAAT CAAATACTGT TGTTCTCCAA GAGCCAGTGC AGGAACAGCC AGGCACCCTA 
ACCATCACAG TCCTTCGCCC TTTCCATGAA GCATCACCAT GGCAAGTAAA AAAGGAAGAT 
ACAATTGTCC CTCCTAATCT CCGGTCAAGG TCATGGGAAA CATTTCCCTC AGTTGAGAAA 
AAAACTCCAG GACCTCCGTT TGGTACTCAG ATTTGGAAAG GCCTTTGCAT TGAGTCCAAA 
CAGCTAGTGA GTGCCCCGGG TGCCATACTC AAGAGCCCCA GTGTGCTACA GATGCGCCAA 
TTCATTCAGC ATGAGGTCAT AAAGAATGAG GTGAAACTGA GGCTGCAGGT TTCCCTTAAG 
GATTACATAA ACTATATTCT AAAGAAAGAA GATGAGCTGC AGGAAATGAT GGTTAAAGAT 
TCCAGAACTG AGGAGGAGAG AGGCAATGCT GCTGATCTCC TCAAGCTGGT TATGTCTTTC 
CCTAAAATGG AGGAAACTAC AAAAAGTCAT GTTACTGAAG TTGCAGCTCA CCTAACTGAT 
TTATTCAAGC AGTCTTTGGC TACGCCTCCA AAATGCCCAC CAGACACATT TGGTAAAGAT 
TTCTTTGAAA AGACATGGAG ACACACAGCA TCCTCAAAAC GCTGGAAAGA AAAGATAGAC 
AAAACGAGGA AGGAAATTGA GACAACACAG AATTACCTAA TGGATATTAA GAACCGCATA 
ATACCACCCT TTTTTAAATC TGAATTGAAC CAGTTATATC AGTCTCAGTA TAATCACCTG 
GACAGTCTTT CCAAAAAACT GCCTTCTTTT ACAAAGAAAA ATGAAGATGC AAACGAAACA 
GCTGTTCAAG ATACATCTGA TCTTAATCTA GATTTCAAGC CACATAAGGT TTCAGAACAG 
AAATCCTCAA GTGTGCCTAG TCTTCCAAAA CCAGAGATTT CTGCAGATAA GAAGGATTTC 
ACTGCTCAGA ACCAAACTGA AAATTTAACA AAATCTCCTG AAGAAGCAGA ATCTTATGAG 
CCCGTGAAAA TTCCAACCCA GTCCTTGCTG CAGGATGTTG CGGGACAAAC AAGAAAAGAA 
AAAGTGAAGT TGCCTCATTA TTTGCTGAGT TCCAAGCCTA AGTCTCAACC TCTTGCAAAG 
GTGCAAGATT CTGTTGGAGG AAAAGTGAAC ACATCCTCTG TTGCATCTGC TGCCATTAAT 
AATGCAAAGT CATCCCTTTT TGGGTTCCAT CTTCTCCCAT CATCAGTCAA GTTTGGAGTG 
CTTAAGGAAG GACATACCTA TGCCACAGTT GTAAAGCTCA AGAATGTTGG AGTGGACTTC 
TGCAGGTTTA AAGTAAAGCA GCCCCCACCC AGCACAGGAC TGAAAGTGAC TTACAAACCT 
GGACCTGTGG CAGCTGGTAT GCAGACAGAA CTGAATATAG AGTTATTTGC CACAGCTGTT 
GGAGAGGATG GGGCCAAGGG ATCAGCACAC ATCTCTCACA ATATCGAGAT TATGACAGAG 
CATGAGGTTC TGTTCCTACC TGTGGAAGCA ACTGTTTTAA CAAGCAGCAA TTATGATAAA 
CGACCAAAAG ACTTTCCCCA GGGAAAAGAA AATCCAATGG TCCAGAGAAC TTCTACAATT 
TATTCCTCCA CACTTGGAGT CTTCATGTCT CGTAAAGTTT CTCCACATTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011539241.1
CDS	359..4849
Translation	MTRIGPWSPEQTTNNEIKDDAVTKADSHEKKPKKMMVEADLEDIKKTQQRSLMDWSFTEHFKPKVLLQVLQEAHKQYRCVDSYYHTQDNSLLLVFHNPMNRQRLHCEYWNIALHSNVGFRNYLELVAKSIQDWITKEEAIYQESKMNEKIIRTRAELELKSSANAKLTSASKIFSIKESKSNKGISKTEISDQEKEKEKEKIPFILEGSLKAWKEEQHRLAEEERLREEKKAEKKGKEAGKKKGKDNAEKEDSRSLKKKSPYKEKSKEEQVKIQEVTEESPHQPEPKITYPFHGYNMGNIPTQISGSNYYLYPSDGGQIEVEKTMFEKGPTFIKVRVVKDNHNFMIHLNDPKEIVKKEEKGDYYLEEEEEGDEEQSLETEVSDAKNKAFSKFGSFSATLENGICLSISYYGSNGMAPEDKDPDLETILNIPSALTPTVVPVIVTVPQSKAKGKIKGKEKPKESLKEEEHPKEEEKKEEEVEPEPVLQETLDVPTFQSLNVSCPSGLLLTFIGQESTGQYVIDEEPTWDIMVRQSYPQRVKHYEFYKTVMPPAEQEASRVITSQGTVVKYMLDGSTQILFADGAVSRSPNSGLICPPSEMPATPHSGDLMDSISQQKSETIPSEITNTKKGKSHKSQSSMAHKGEIHDPPPEAVQTVTPVEVHIGTWFTTTPEGNRIGTKGLERIADLTPLLSFQATDPVNGTVMTTREDKVVIVERKDGTRIVDHADGTRITTFYQVYEDQIILPDDQETTEGPRTVTRQVKCMRVESSRYATVIANCEDSSCCATFGDGTTIIAKPQGTYQVLPPNTGSLYIDKDCSAVYCHESSSNIYYPFQKREQLRAGRYIMRHTSEVICEVLDPEGNTFQVMADGSISTILPEKKLEDDLNEKTEGYDSLSSMHLEKNHQQIYGEHVPRFFVMYADGSGMELLRDSDIEEYLSLAYKESNTVVLQEPVQEQPGTLTITVLRPFHEASPWQVKKEDTIVPPNLRSRSWETFPSVEKKTPGPPFGTQIWKGLCIESKQLVSAPGAILKSPSVLQMRQFIQHEVIKNEVKLRLQVSLKDYINYILKKEDELQEMMVKDSRTEEERGNAADLLKLVMSFPKMEETTKSHVTEVAAHLTDLFKQSLATPPKCPPDTFGKDFFEKTWRHTASSKRWKEKIDKTRKEIETTQNYLMDIKNRIIPPFFKSELNQLYQSQYNHLDSLSKKLPSFTKKNEDANETAVQDTSDLNLDFKPHKVSEQKSSSVPSLPKPEISADKKDFTAQNQTENLTKSPEEAESYEPVKIPTQSLLQDVAGQTRKEKVKLPHYLLSSKPKSQPLAKVQDSVGGKVNTSSVASAAINNAKSSLFGFHLLPSSVKFGVLKEGHTYATVVKLKNVGVDFCRFKVKQPPPSTGLKVTYKPGPVAAGMQTELNIELFATAVGEDGAKGSAHISHNIEIMTEHEVLFLPVEATVLTSSNYDKRPKDFPQGKENPMVQRTSTIYSSTLGVFMSRKVSPH

Target ORF information:

RefSeq Version	XM_011540939.2
Organism	Homo sapiens(human)
Definition	Homo sapiens sperm associated antigen 17 (SPAG17), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011540939.2

ORF Insert Sequence:

ATGACTCGTA TTGGTCCATG GAGTCCAGAG CAGACCACAA ACAATGAGAT CAAAGATGAT 
GCAGTCACAA AGGCTGATTC TCATGAAAAG AAACCCAAGA AGATGATGGT GGAAGCAGAT 
TTAGAGGACA TAAAGAAAAC ACAGCAGCGC AGTCTAATGG ACTGGAGTTT TACTGAACAT 
TTTAAACCGA AAGTACTGCT TCAGGTCCTT CAAGAAGCCC ATAAGCAATA TAGGTGTGTT 
GATTCTTACT ACCACACCCA AGACAACTCT TTACTTTTAG TCTTTCACAA TCCAATGAAT 
AGACAACGTT TGCATTGTGA ATATTGGAAC ATTGCTCTCC ACTCCAATGT TGGATTCAGG 
AATTATTTGG AACTTGTTGC AAAATCTATT CAAGATTGGA TTACAAAAGA AGAAGCTATA 
TATCAGGAAT CTAAAATGAA TGAGAAAATC ATCAGGACCA GAGCTGAGCT GGAATTGAAA 
TCTTCTGCTA ATGCCAAACT TACTTCTGCT AGCAAAATTT TTTCCATTAA AGAATCTAAA 
AGTAACAAAG GAATCAGCAA AACAGAGATA TCAGATCAAG AAAAAGAAAA AGAGAAGGAA 
AAGATTCCTT TCATTTTAGA AGGCTCTCTC AAGGCATGGA AAGAAGAGCA ACATCGATTA 
GCAGAAGAGG AGCGCTTAAG GGAAGAAAAG AAAGCAGAGA AGAAGGGTAA AGAAGCTGGT 
AAAAAGAAAG GCAAGGATAA CGCAGAGAAA GAGGATAGTA GGTCTTTGAA GAAAAAATCA 
CCTTACAAGG AGAAATCTAA AGAAGAACAA GTCAAGATCC AAGAAGTAAC AGAAGAGTCC 
CCCCACCAAC CAGAACCTAA GATAACTTAC CCGTTTCACG GATACAATAT GGGAAATATA 
CCCACTCAAA TCTCAGGGTC AAATTACTAC CTGTATCCTT CTGATGGGGG GCAGATTGAA 
GTGGAAAAGA CAATGTTTGA AAAAGGCCCA ACTTTTATCA AAGTGAGAGT GGTAAAGGAC 
AACCACAATT TTATGATTCA TTTAAATGAC CCTAAGGAAA TTGTGAAAAA GGAAGAGAAA 
GGGGATTATT ATTTAGAAGA GGAAGAAGAA GGAGATGAGG AACAAAGTCT TGAAACGGAA 
GTATCAGATG CAAAGAATAA AGCTTTCAGC AAGTTTGGAT CTTTTTCTGC CACCTTAGAA 
AATGGAATCT GCCTCTCGAT AAGTTACTAT GGATCAAATG GAATGGCACC AGAAGATAAG 
GATCCTGATT TAGAAACAAT ATTGAATATC CCTTCAGCAC TCACTCCAAC AGTGGTTCCT 
GTTATAGTGA CCGTTCCTCA AAGCAAAGCT AAAGGGAAAA TAAAAGGCAA AGAAAAACCC 
AAAGAATCCC TTAAAGAAGA AGAACACCCA AAAGAAGAAG AGAAAAAGGA AGAAGAAGTA 
GAACCAGAAC CTGTTTTACA AGAGACTTTG GATGTTCCCA CCTTCCAGAG CCTAAATGTG 
TCTTGCCCCA GTGGGCTCCT GTTGACTTTC ATTGGACAAG AATCTACAGG TCAATATGTT 
ATAGATGAGG AACCCACCTG GGACATCATG GTCCGTCAGA GCTACCCCCA GAGGGTGAAG 
CACTATGAGT TCTATAAAAC GGTGATGCCA CCCGCAGAGC AGGAGGCTTC AAGGGTTATC 
ACCAGTCAAG GCACTGTTGT CAAATATATG TTGGATGGAT CCACACAGAT TCTCTTTGCA 
GATGGTGCTG TGAGCAGGAG TCCCAATTCA GGTCTTATTT GTCCTCCTTC TGAAATGCCA 
GCAACGCCTC ACAGTGGAGA TTTGATGGAC TCTATTTCTC AGCAGAAATC AGAAACGATA 
CCATCTGAGA TTACCAACAC AAAGAAAGGA AAAAGTCACA AAAGTCAGTC ATCAATGGCC 
CATAAGGGTG AAATCCATGA CCCTCCTCCA GAGGCAGTTC AAACTGTAAC TCCTGTGGAG 
GTTCACATAG GCACCTGGTT TACAACCACA CCTGAAGGAA ATCGGATCGG CACCAAAGGA 
TTAGAAAGAA TAGCAGACTT GACCCCATTG TTATCCTTTC AGGCCACAGA TCCTGTCAAT 
GGAACGGTTA TGACAACTCG AGAAGACAAA GTTGTCATAG TTGAAAGGAA AGATGGTACT 
CGGATAGTGG ATCATGCTGA TGGTACCAGA ATCACAACCT TTTATCAAGT TTATGAAGAT 
CAAATTATTC TGCCAGATGA TCAAGAAACA ACCGAGGGTC CTCGGACTGT CACCAGGCAG 
GTGAAGTGTA TGCGGGTAGA AAGCTCACGC TATGCCACTG TTATCGCCAA CTGTGAGGAC 
AGTAGCTGCT GTGCCACCTT TGGAGATGGA ACAACTATTA TTGCAAAGCC ACAGGGAACA 
TACCAGGTGT TACCTCCAAA CACAGGCTCT CTTTATATTG ACAAGGATTG TTCAGCTGTG 
TACTGCCATG AGTCAAGCAG TAATATATAC TATCCTTTTC AAAAGCGTGA GCAGCTGCGA 
GCTGGCAGGT ACATCATGAG GCATACTTCA GAGGTTATCT GTGAGGTTCT GGATCCTGAG 
GGAAACACTT TTCAGGTCAT GGCTGATGGT AGCATATCAA CTATATTACC TGAAAAAAAA 
TTGGAAGATG ATTTAAATGA GAAAACTGAG GGCTATGATA GTCTGTCCTC TATGCACCTT 
GAAAAGAATC ATCAGCAAAT CTATGGTGAA CATGTCCCCA GGTTTTTTGT TATGTATGCT 
GATGGATCAG GAATGGAACT TCTTCGAGAC AGTGACATAG AAGAATATCT ATCTTTGGCA 
TATAAAGAAT CAAATACTGT TGTTCTCCAA GAGCCAGTGC AGGAACAGCC AGGCACCCTA 
ACCATCACAG TCCTTCGCCC TTTCCATGAA GCATCACCAT GGCAAGTAAA AAAGGAAGAT 
ACAATTGTCC CTCCTAATCT CCGGTCAAGG TCATGGGAAA CATTTCCCTC AGTTGAGAAA 
AAAACTCCAG GACCTCCGTT TGGTACTCAG ATTTGGAAAG GCCTTTGCAT TGAGTCCAAA 
CAGCTAGTGA GTGCCCCGGG TGCCATACTC AAGAGCCCCA GTGTGCTACA GATGCGCCAA 
TTCATTCAGC ATGAGGTCAT AAAGAATGAG GTGAAACTGA GGCTGCAGGT TTCCCTTAAG 
GATTACATAA ACTATATTCT AAAGAAAGAA GATGAGCTGC AGGAAATGAT GGTTAAAGAT 
TCCAGAACTG AGGAGGAGAG AGGCAATGCT GCTGATCTCC TCAAGCTGGT TATGTCTTTC 
CCTAAAATGG AGGAAACTAC AAAAAGTCAT GTTACTGAAG TTGCAGCTCA CCTAACTGAT 
TTATTCAAGC AGTCTTTGGC TACGCCTCCA AAATGCCCAC CAGACACATT TGGTAAAGAT 
TTCTTTGAAA AGACATGGAG ACACACAGCA TCCTCAAAAC GCTGGAAAGA AAAGATAGAC 
AAAACGAGGA AGGAAATTGA GACAACACAG AATTACCTAA TGGATATTAA GAACCGCATA 
ATACCACCCT TTTTTAAATC TGAATTGAAC CAGTTATATC AGTCTCAGTA TAATCACCTG 
GACAGTCTTT CCAAAAAACT GCCTTCTTTT ACAAAGAAAA ATGAAGATGC AAACGAAACA 
GCTGTTCAAG ATACATCTGA TCTTAATCTA GATTTCAAGC CACATAAGGT TTCAGAACAG 
AAATCCTCAA GTGTGCCTAG TCTTCCAAAA CCAGAGATTT CTGCAGATAA GAAGGATTTC 
ACTGCTCAGA ACCAAACTGA AAATTTAACA AAATCTCCTG AAGAAGCAGA ATCTTATGAG 
CCCGTGAAAA TTCCAACCCA GTCCTTGCTG CAGGATGTTG CGGGACAAAC AAGAAAAGAA 
AAAGTGAAGT TGCCTCATTA TTTGCTGAGT TCCAAGCCTA AGTCTCAACC TCTTGCAAAG 
GTGCAAGATT CTGTTGGAGG AAAAGTGAAC ACATCCTCTG TTGCATCTGC TGCCATTAAT 
AATGCAAAGT CATCCCTTTT TGGGTTCCAT CTTCTCCCAT CATCAGTCAA GTTTGGAGTG 
CTTAAGGAAG GACATACCTA TGCCACAGTT GTAAAGCTCA AGAATGTTGG AGTGGACTTC 
TGCAGGTTTA AAGTAAAGCA GCCCCCACCC AGCACAGGAC TGAAAGTGAC TTACAAACCT 
GGACCTGTGG CAGCTGGTAT GCAGACAGAA CTGAATATAG AGTTATTTGC CACAGCTGTT 
GGAGAGGATG GGGCCAAGGG ATCAGCACAC ATCTCTCACA ATATCGAGAT TATGACAGAG 
CATGAGGTTC TGTTCCTACC TGTGGAAGCA ACTGTTTTAA CAAGCAGCAA TTATGATAAA 
CGACCAAAAG ACTTTCCCCA GGGAAAAGAA AATCCAATGG TCCAGAGAAC TTCTACAATT 
TATTCCTCCA CACTTGGAGT CTTCATGTCT CGTAAAGTTT CTCCACATTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPAG17 ( XM_006710428.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_006710491.1 Homo sapiens sperm associated antigen 17 (SPAG17), transcript variant X9, mRNA.

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CloneID	OHu33133
Clone ID Related Accession (Same CDS sequence)	XM_011540939.2 , XM_006710428.3
Accession Version	XM_006710428.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4491bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	sperm-associated antigen 17 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006710428.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTCGTA TTGGTCCATG GAGTCCAGAG CAGACCACAA ACAATGAGAT CAAAGATGAT 
GCAGTCACAA AGGCTGATTC TCATGAAAAG AAACCCAAGA AGATGATGGT GGAAGCAGAT 
TTAGAGGACA TAAAGAAAAC ACAGCAGCGC AGTCTAATGG ACTGGAGTTT TACTGAACAT 
TTTAAACCGA AAGTACTGCT TCAGGTCCTT CAAGAAGCCC ATAAGCAATA TAGGTGTGTT 
GATTCTTACT ACCACACCCA AGACAACTCT TTACTTTTAG TCTTTCACAA TCCAATGAAT 
AGACAACGTT TGCATTGTGA ATATTGGAAC ATTGCTCTCC ACTCCAATGT TGGATTCAGG 
AATTATTTGG AACTTGTTGC AAAATCTATT CAAGATTGGA TTACAAAAGA AGAAGCTATA 
TATCAGGAAT CTAAAATGAA TGAGAAAATC ATCAGGACCA GAGCTGAGCT GGAATTGAAA 
TCTTCTGCTA ATGCCAAACT TACTTCTGCT AGCAAAATTT TTTCCATTAA AGAATCTAAA 
AGTAACAAAG GAATCAGCAA AACAGAGATA TCAGATCAAG AAAAAGAAAA AGAGAAGGAA 
AAGATTCCTT TCATTTTAGA AGGCTCTCTC AAGGCATGGA AAGAAGAGCA ACATCGATTA 
GCAGAAGAGG AGCGCTTAAG GGAAGAAAAG AAAGCAGAGA AGAAGGGTAA AGAAGCTGGT 
AAAAAGAAAG GCAAGGATAA CGCAGAGAAA GAGGATAGTA GGTCTTTGAA GAAAAAATCA 
CCTTACAAGG AGAAATCTAA AGAAGAACAA GTCAAGATCC AAGAAGTAAC AGAAGAGTCC 
CCCCACCAAC CAGAACCTAA GATAACTTAC CCGTTTCACG GATACAATAT GGGAAATATA 
CCCACTCAAA TCTCAGGGTC AAATTACTAC CTGTATCCTT CTGATGGGGG GCAGATTGAA 
GTGGAAAAGA CAATGTTTGA AAAAGGCCCA ACTTTTATCA AAGTGAGAGT GGTAAAGGAC 
AACCACAATT TTATGATTCA TTTAAATGAC CCTAAGGAAA TTGTGAAAAA GGAAGAGAAA 
GGGGATTATT ATTTAGAAGA GGAAGAAGAA GGAGATGAGG AACAAAGTCT TGAAACGGAA 
GTATCAGATG CAAAGAATAA AGCTTTCAGC AAGTTTGGAT CTTTTTCTGC CACCTTAGAA 
AATGGAATCT GCCTCTCGAT AAGTTACTAT GGATCAAATG GAATGGCACC AGAAGATAAG 
GATCCTGATT TAGAAACAAT ATTGAATATC CCTTCAGCAC TCACTCCAAC AGTGGTTCCT 
GTTATAGTGA CCGTTCCTCA AAGCAAAGCT AAAGGGAAAA TAAAAGGCAA AGAAAAACCC 
AAAGAATCCC TTAAAGAAGA AGAACACCCA AAAGAAGAAG AGAAAAAGGA AGAAGAAGTA 
GAACCAGAAC CTGTTTTACA AGAGACTTTG GATGTTCCCA CCTTCCAGAG CCTAAATGTG 
TCTTGCCCCA GTGGGCTCCT GTTGACTTTC ATTGGACAAG AATCTACAGG TCAATATGTT 
ATAGATGAGG AACCCACCTG GGACATCATG GTCCGTCAGA GCTACCCCCA GAGGGTGAAG 
CACTATGAGT TCTATAAAAC GGTGATGCCA CCCGCAGAGC AGGAGGCTTC AAGGGTTATC 
ACCAGTCAAG GCACTGTTGT CAAATATATG TTGGATGGAT CCACACAGAT TCTCTTTGCA 
GATGGTGCTG TGAGCAGGAG TCCCAATTCA GGTCTTATTT GTCCTCCTTC TGAAATGCCA 
GCAACGCCTC ACAGTGGAGA TTTGATGGAC TCTATTTCTC AGCAGAAATC AGAAACGATA 
CCATCTGAGA TTACCAACAC AAAGAAAGGA AAAAGTCACA AAAGTCAGTC ATCAATGGCC 
CATAAGGGTG AAATCCATGA CCCTCCTCCA GAGGCAGTTC AAACTGTAAC TCCTGTGGAG 
GTTCACATAG GCACCTGGTT TACAACCACA CCTGAAGGAA ATCGGATCGG CACCAAAGGA 
TTAGAAAGAA TAGCAGACTT GACCCCATTG TTATCCTTTC AGGCCACAGA TCCTGTCAAT 
GGAACGGTTA TGACAACTCG AGAAGACAAA GTTGTCATAG TTGAAAGGAA AGATGGTACT 
CGGATAGTGG ATCATGCTGA TGGTACCAGA ATCACAACCT TTTATCAAGT TTATGAAGAT 
CAAATTATTC TGCCAGATGA TCAAGAAACA ACCGAGGGTC CTCGGACTGT CACCAGGCAG 
GTGAAGTGTA TGCGGGTAGA AAGCTCACGC TATGCCACTG TTATCGCCAA CTGTGAGGAC 
AGTAGCTGCT GTGCCACCTT TGGAGATGGA ACAACTATTA TTGCAAAGCC ACAGGGAACA 
TACCAGGTGT TACCTCCAAA CACAGGCTCT CTTTATATTG ACAAGGATTG TTCAGCTGTG 
TACTGCCATG AGTCAAGCAG TAATATATAC TATCCTTTTC AAAAGCGTGA GCAGCTGCGA 
GCTGGCAGGT ACATCATGAG GCATACTTCA GAGGTTATCT GTGAGGTTCT GGATCCTGAG 
GGAAACACTT TTCAGGTCAT GGCTGATGGT AGCATATCAA CTATATTACC TGAAAAAAAA 
TTGGAAGATG ATTTAAATGA GAAAACTGAG GGCTATGATA GTCTGTCCTC TATGCACCTT 
GAAAAGAATC ATCAGCAAAT CTATGGTGAA CATGTCCCCA GGTTTTTTGT TATGTATGCT 
GATGGATCAG GAATGGAACT TCTTCGAGAC AGTGACATAG AAGAATATCT ATCTTTGGCA 
TATAAAGAAT CAAATACTGT TGTTCTCCAA GAGCCAGTGC AGGAACAGCC AGGCACCCTA 
ACCATCACAG TCCTTCGCCC TTTCCATGAA GCATCACCAT GGCAAGTAAA AAAGGAAGAT 
ACAATTGTCC CTCCTAATCT CCGGTCAAGG TCATGGGAAA CATTTCCCTC AGTTGAGAAA 
AAAACTCCAG GACCTCCGTT TGGTACTCAG ATTTGGAAAG GCCTTTGCAT TGAGTCCAAA 
CAGCTAGTGA GTGCCCCGGG TGCCATACTC AAGAGCCCCA GTGTGCTACA GATGCGCCAA 
TTCATTCAGC ATGAGGTCAT AAAGAATGAG GTGAAACTGA GGCTGCAGGT TTCCCTTAAG 
GATTACATAA ACTATATTCT AAAGAAAGAA GATGAGCTGC AGGAAATGAT GGTTAAAGAT 
TCCAGAACTG AGGAGGAGAG AGGCAATGCT GCTGATCTCC TCAAGCTGGT TATGTCTTTC 
CCTAAAATGG AGGAAACTAC AAAAAGTCAT GTTACTGAAG TTGCAGCTCA CCTAACTGAT 
TTATTCAAGC AGTCTTTGGC TACGCCTCCA AAATGCCCAC CAGACACATT TGGTAAAGAT 
TTCTTTGAAA AGACATGGAG ACACACAGCA TCCTCAAAAC GCTGGAAAGA AAAGATAGAC 
AAAACGAGGA AGGAAATTGA GACAACACAG AATTACCTAA TGGATATTAA GAACCGCATA 
ATACCACCCT TTTTTAAATC TGAATTGAAC CAGTTATATC AGTCTCAGTA TAATCACCTG 
GACAGTCTTT CCAAAAAACT GCCTTCTTTT ACAAAGAAAA ATGAAGATGC AAACGAAACA 
GCTGTTCAAG ATACATCTGA TCTTAATCTA GATTTCAAGC CACATAAGGT TTCAGAACAG 
AAATCCTCAA GTGTGCCTAG TCTTCCAAAA CCAGAGATTT CTGCAGATAA GAAGGATTTC 
ACTGCTCAGA ACCAAACTGA AAATTTAACA AAATCTCCTG AAGAAGCAGA ATCTTATGAG 
CCCGTGAAAA TTCCAACCCA GTCCTTGCTG CAGGATGTTG CGGGACAAAC AAGAAAAGAA 
AAAGTGAAGT TGCCTCATTA TTTGCTGAGT TCCAAGCCTA AGTCTCAACC TCTTGCAAAG 
GTGCAAGATT CTGTTGGAGG AAAAGTGAAC ACATCCTCTG TTGCATCTGC TGCCATTAAT 
AATGCAAAGT CATCCCTTTT TGGGTTCCAT CTTCTCCCAT CATCAGTCAA GTTTGGAGTG 
CTTAAGGAAG GACATACCTA TGCCACAGTT GTAAAGCTCA AGAATGTTGG AGTGGACTTC 
TGCAGGTTTA AAGTAAAGCA GCCCCCACCC AGCACAGGAC TGAAAGTGAC TTACAAACCT 
GGACCTGTGG CAGCTGGTAT GCAGACAGAA CTGAATATAG AGTTATTTGC CACAGCTGTT 
GGAGAGGATG GGGCCAAGGG ATCAGCACAC ATCTCTCACA ATATCGAGAT TATGACAGAG 
CATGAGGTTC TGTTCCTACC TGTGGAAGCA ACTGTTTTAA CAAGCAGCAA TTATGATAAA 
CGACCAAAAG ACTTTCCCCA GGGAAAAGAA AATCCAATGG TCCAGAGAAC TTCTACAATT 
TATTCCTCCA CACTTGGAGT CTTCATGTCT CGTAAAGTTT CTCCACATTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006710491.1
CDS	321..4811
Translation	MTRIGPWSPEQTTNNEIKDDAVTKADSHEKKPKKMMVEADLEDIKKTQQRSLMDWSFTEHFKPKVLLQVLQEAHKQYRCVDSYYHTQDNSLLLVFHNPMNRQRLHCEYWNIALHSNVGFRNYLELVAKSIQDWITKEEAIYQESKMNEKIIRTRAELELKSSANAKLTSASKIFSIKESKSNKGISKTEISDQEKEKEKEKIPFILEGSLKAWKEEQHRLAEEERLREEKKAEKKGKEAGKKKGKDNAEKEDSRSLKKKSPYKEKSKEEQVKIQEVTEESPHQPEPKITYPFHGYNMGNIPTQISGSNYYLYPSDGGQIEVEKTMFEKGPTFIKVRVVKDNHNFMIHLNDPKEIVKKEEKGDYYLEEEEEGDEEQSLETEVSDAKNKAFSKFGSFSATLENGICLSISYYGSNGMAPEDKDPDLETILNIPSALTPTVVPVIVTVPQSKAKGKIKGKEKPKESLKEEEHPKEEEKKEEEVEPEPVLQETLDVPTFQSLNVSCPSGLLLTFIGQESTGQYVIDEEPTWDIMVRQSYPQRVKHYEFYKTVMPPAEQEASRVITSQGTVVKYMLDGSTQILFADGAVSRSPNSGLICPPSEMPATPHSGDLMDSISQQKSETIPSEITNTKKGKSHKSQSSMAHKGEIHDPPPEAVQTVTPVEVHIGTWFTTTPEGNRIGTKGLERIADLTPLLSFQATDPVNGTVMTTREDKVVIVERKDGTRIVDHADGTRITTFYQVYEDQIILPDDQETTEGPRTVTRQVKCMRVESSRYATVIANCEDSSCCATFGDGTTIIAKPQGTYQVLPPNTGSLYIDKDCSAVYCHESSSNIYYPFQKREQLRAGRYIMRHTSEVICEVLDPEGNTFQVMADGSISTILPEKKLEDDLNEKTEGYDSLSSMHLEKNHQQIYGEHVPRFFVMYADGSGMELLRDSDIEEYLSLAYKESNTVVLQEPVQEQPGTLTITVLRPFHEASPWQVKKEDTIVPPNLRSRSWETFPSVEKKTPGPPFGTQIWKGLCIESKQLVSAPGAILKSPSVLQMRQFIQHEVIKNEVKLRLQVSLKDYINYILKKEDELQEMMVKDSRTEEERGNAADLLKLVMSFPKMEETTKSHVTEVAAHLTDLFKQSLATPPKCPPDTFGKDFFEKTWRHTASSKRWKEKIDKTRKEIETTQNYLMDIKNRIIPPFFKSELNQLYQSQYNHLDSLSKKLPSFTKKNEDANETAVQDTSDLNLDFKPHKVSEQKSSSVPSLPKPEISADKKDFTAQNQTENLTKSPEEAESYEPVKIPTQSLLQDVAGQTRKEKVKLPHYLLSSKPKSQPLAKVQDSVGGKVNTSSVASAAINNAKSSLFGFHLLPSSVKFGVLKEGHTYATVVKLKNVGVDFCRFKVKQPPPSTGLKVTYKPGPVAAGMQTELNIELFATAVGEDGAKGSAHISHNIEIMTEHEVLFLPVEATVLTSSNYDKRPKDFPQGKENPMVQRTSTIYSSTLGVFMSRKVSPH

Target ORF information:

RefSeq Version	XM_006710428.3
Organism	Homo sapiens(human)
Definition	Homo sapiens sperm associated antigen 17 (SPAG17), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006710428.3

ORF Insert Sequence:

ATGACTCGTA TTGGTCCATG GAGTCCAGAG CAGACCACAA ACAATGAGAT CAAAGATGAT 
GCAGTCACAA AGGCTGATTC TCATGAAAAG AAACCCAAGA AGATGATGGT GGAAGCAGAT 
TTAGAGGACA TAAAGAAAAC ACAGCAGCGC AGTCTAATGG ACTGGAGTTT TACTGAACAT 
TTTAAACCGA AAGTACTGCT TCAGGTCCTT CAAGAAGCCC ATAAGCAATA TAGGTGTGTT 
GATTCTTACT ACCACACCCA AGACAACTCT TTACTTTTAG TCTTTCACAA TCCAATGAAT 
AGACAACGTT TGCATTGTGA ATATTGGAAC ATTGCTCTCC ACTCCAATGT TGGATTCAGG 
AATTATTTGG AACTTGTTGC AAAATCTATT CAAGATTGGA TTACAAAAGA AGAAGCTATA 
TATCAGGAAT CTAAAATGAA TGAGAAAATC ATCAGGACCA GAGCTGAGCT GGAATTGAAA 
TCTTCTGCTA ATGCCAAACT TACTTCTGCT AGCAAAATTT TTTCCATTAA AGAATCTAAA 
AGTAACAAAG GAATCAGCAA AACAGAGATA TCAGATCAAG AAAAAGAAAA AGAGAAGGAA 
AAGATTCCTT TCATTTTAGA AGGCTCTCTC AAGGCATGGA AAGAAGAGCA ACATCGATTA 
GCAGAAGAGG AGCGCTTAAG GGAAGAAAAG AAAGCAGAGA AGAAGGGTAA AGAAGCTGGT 
AAAAAGAAAG GCAAGGATAA CGCAGAGAAA GAGGATAGTA GGTCTTTGAA GAAAAAATCA 
CCTTACAAGG AGAAATCTAA AGAAGAACAA GTCAAGATCC AAGAAGTAAC AGAAGAGTCC 
CCCCACCAAC CAGAACCTAA GATAACTTAC CCGTTTCACG GATACAATAT GGGAAATATA 
CCCACTCAAA TCTCAGGGTC AAATTACTAC CTGTATCCTT CTGATGGGGG GCAGATTGAA 
GTGGAAAAGA CAATGTTTGA AAAAGGCCCA ACTTTTATCA AAGTGAGAGT GGTAAAGGAC 
AACCACAATT TTATGATTCA TTTAAATGAC CCTAAGGAAA TTGTGAAAAA GGAAGAGAAA 
GGGGATTATT ATTTAGAAGA GGAAGAAGAA GGAGATGAGG AACAAAGTCT TGAAACGGAA 
GTATCAGATG CAAAGAATAA AGCTTTCAGC AAGTTTGGAT CTTTTTCTGC CACCTTAGAA 
AATGGAATCT GCCTCTCGAT AAGTTACTAT GGATCAAATG GAATGGCACC AGAAGATAAG 
GATCCTGATT TAGAAACAAT ATTGAATATC CCTTCAGCAC TCACTCCAAC AGTGGTTCCT 
GTTATAGTGA CCGTTCCTCA AAGCAAAGCT AAAGGGAAAA TAAAAGGCAA AGAAAAACCC 
AAAGAATCCC TTAAAGAAGA AGAACACCCA AAAGAAGAAG AGAAAAAGGA AGAAGAAGTA 
GAACCAGAAC CTGTTTTACA AGAGACTTTG GATGTTCCCA CCTTCCAGAG CCTAAATGTG 
TCTTGCCCCA GTGGGCTCCT GTTGACTTTC ATTGGACAAG AATCTACAGG TCAATATGTT 
ATAGATGAGG AACCCACCTG GGACATCATG GTCCGTCAGA GCTACCCCCA GAGGGTGAAG 
CACTATGAGT TCTATAAAAC GGTGATGCCA CCCGCAGAGC AGGAGGCTTC AAGGGTTATC 
ACCAGTCAAG GCACTGTTGT CAAATATATG TTGGATGGAT CCACACAGAT TCTCTTTGCA 
GATGGTGCTG TGAGCAGGAG TCCCAATTCA GGTCTTATTT GTCCTCCTTC TGAAATGCCA 
GCAACGCCTC ACAGTGGAGA TTTGATGGAC TCTATTTCTC AGCAGAAATC AGAAACGATA 
CCATCTGAGA TTACCAACAC AAAGAAAGGA AAAAGTCACA AAAGTCAGTC ATCAATGGCC 
CATAAGGGTG AAATCCATGA CCCTCCTCCA GAGGCAGTTC AAACTGTAAC TCCTGTGGAG 
GTTCACATAG GCACCTGGTT TACAACCACA CCTGAAGGAA ATCGGATCGG CACCAAAGGA 
TTAGAAAGAA TAGCAGACTT GACCCCATTG TTATCCTTTC AGGCCACAGA TCCTGTCAAT 
GGAACGGTTA TGACAACTCG AGAAGACAAA GTTGTCATAG TTGAAAGGAA AGATGGTACT 
CGGATAGTGG ATCATGCTGA TGGTACCAGA ATCACAACCT TTTATCAAGT TTATGAAGAT 
CAAATTATTC TGCCAGATGA TCAAGAAACA ACCGAGGGTC CTCGGACTGT CACCAGGCAG 
GTGAAGTGTA TGCGGGTAGA AAGCTCACGC TATGCCACTG TTATCGCCAA CTGTGAGGAC 
AGTAGCTGCT GTGCCACCTT TGGAGATGGA ACAACTATTA TTGCAAAGCC ACAGGGAACA 
TACCAGGTGT TACCTCCAAA CACAGGCTCT CTTTATATTG ACAAGGATTG TTCAGCTGTG 
TACTGCCATG AGTCAAGCAG TAATATATAC TATCCTTTTC AAAAGCGTGA GCAGCTGCGA 
GCTGGCAGGT ACATCATGAG GCATACTTCA GAGGTTATCT GTGAGGTTCT GGATCCTGAG 
GGAAACACTT TTCAGGTCAT GGCTGATGGT AGCATATCAA CTATATTACC TGAAAAAAAA 
TTGGAAGATG ATTTAAATGA GAAAACTGAG GGCTATGATA GTCTGTCCTC TATGCACCTT 
GAAAAGAATC ATCAGCAAAT CTATGGTGAA CATGTCCCCA GGTTTTTTGT TATGTATGCT 
GATGGATCAG GAATGGAACT TCTTCGAGAC AGTGACATAG AAGAATATCT ATCTTTGGCA 
TATAAAGAAT CAAATACTGT TGTTCTCCAA GAGCCAGTGC AGGAACAGCC AGGCACCCTA 
ACCATCACAG TCCTTCGCCC TTTCCATGAA GCATCACCAT GGCAAGTAAA AAAGGAAGAT 
ACAATTGTCC CTCCTAATCT CCGGTCAAGG TCATGGGAAA CATTTCCCTC AGTTGAGAAA 
AAAACTCCAG GACCTCCGTT TGGTACTCAG ATTTGGAAAG GCCTTTGCAT TGAGTCCAAA 
CAGCTAGTGA GTGCCCCGGG TGCCATACTC AAGAGCCCCA GTGTGCTACA GATGCGCCAA 
TTCATTCAGC ATGAGGTCAT AAAGAATGAG GTGAAACTGA GGCTGCAGGT TTCCCTTAAG 
GATTACATAA ACTATATTCT AAAGAAAGAA GATGAGCTGC AGGAAATGAT GGTTAAAGAT 
TCCAGAACTG AGGAGGAGAG AGGCAATGCT GCTGATCTCC TCAAGCTGGT TATGTCTTTC 
CCTAAAATGG AGGAAACTAC AAAAAGTCAT GTTACTGAAG TTGCAGCTCA CCTAACTGAT 
TTATTCAAGC AGTCTTTGGC TACGCCTCCA AAATGCCCAC CAGACACATT TGGTAAAGAT 
TTCTTTGAAA AGACATGGAG ACACACAGCA TCCTCAAAAC GCTGGAAAGA AAAGATAGAC 
AAAACGAGGA AGGAAATTGA GACAACACAG AATTACCTAA TGGATATTAA GAACCGCATA 
ATACCACCCT TTTTTAAATC TGAATTGAAC CAGTTATATC AGTCTCAGTA TAATCACCTG 
GACAGTCTTT CCAAAAAACT GCCTTCTTTT ACAAAGAAAA ATGAAGATGC AAACGAAACA 
GCTGTTCAAG ATACATCTGA TCTTAATCTA GATTTCAAGC CACATAAGGT TTCAGAACAG 
AAATCCTCAA GTGTGCCTAG TCTTCCAAAA CCAGAGATTT CTGCAGATAA GAAGGATTTC 
ACTGCTCAGA ACCAAACTGA AAATTTAACA AAATCTCCTG AAGAAGCAGA ATCTTATGAG 
CCCGTGAAAA TTCCAACCCA GTCCTTGCTG CAGGATGTTG CGGGACAAAC AAGAAAAGAA 
AAAGTGAAGT TGCCTCATTA TTTGCTGAGT TCCAAGCCTA AGTCTCAACC TCTTGCAAAG 
GTGCAAGATT CTGTTGGAGG AAAAGTGAAC ACATCCTCTG TTGCATCTGC TGCCATTAAT 
AATGCAAAGT CATCCCTTTT TGGGTTCCAT CTTCTCCCAT CATCAGTCAA GTTTGGAGTG 
CTTAAGGAAG GACATACCTA TGCCACAGTT GTAAAGCTCA AGAATGTTGG AGTGGACTTC 
TGCAGGTTTA AAGTAAAGCA GCCCCCACCC AGCACAGGAC TGAAAGTGAC TTACAAACCT 
GGACCTGTGG CAGCTGGTAT GCAGACAGAA CTGAATATAG AGTTATTTGC CACAGCTGTT 
GGAGAGGATG GGGCCAAGGG ATCAGCACAC ATCTCTCACA ATATCGAGAT TATGACAGAG 
CATGAGGTTC TGTTCCTACC TGTGGAAGCA ACTGTTTTAA CAAGCAGCAA TTATGATAAA 
CGACCAAAAG ACTTTCCCCA GGGAAAAGAA AATCCAATGG TCCAGAGAAC TTCTACAATT 
TATTCCTCCA CACTTGGAGT CTTCATGTCT CGTAAAGTTT CTCCACATTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPAG17 ( XM_006710429.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_006710492.1 Homo sapiens sperm associated antigen 17 (SPAG17), transcript variant X12, mRNA.

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CloneID	OHu33134
Clone ID Related Accession (Same CDS sequence)	XM_006710429.3
Accession Version	XM_006710429.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4056bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	sperm-associated antigen 17 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006710429.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATGAGA AAATCATCAG GACCAGAGCT GAGCTGGAAT TGAAATCTTC TGCTAATGCC 
AAACTTACTT CTGCTAGCAA AATTTTTTCC ATTAAAGAAT CTAAAAGTAA CAAAGGAATC 
AGCAAAACAG AGATATCAGA TCAAGAAAAA GAAAAAGAGA AGGAAAAGAT TCCTTTCATT 
TTAGAAGGCT CTCTCAAGGC ATGGAAAGAA GAGCAACATC GATTAGCAGA AGAGGAGCGC 
TTAAGGGAAG AAAAGAAAGC AGAGAAGAAG GGTAAAGAAG CTGGTAAAAA GAAAGGCAAG 
GATAACGCAG AGAAAGAGGA TAGTAGGTCT TTGAAGAAAA AATCACCTTA CAAGGAGAAA 
TCTAAAGAAG AACAAGTCAA GATCCAAGAA GTAACAGAAG AGTCCCCCCA CCAACCAGAA 
CCTAAGATAA CTTACCCGTT TCACGGATAC AATATGGGAA ATATACCCAC TCAAATCTCA 
GGGTCAAATT ACTACCTGTA TCCTTCTGAT GGGGGGCAGA TTGAAGTGGA AAAGACAATG 
TTTGAAAAAG GCCCAACTTT TATCAAAGTG AGAGTGGTAA AGGACAACCA CAATTTTATG 
ATTCATTTAA ATGACCCTAA GGAAATTGTG AAAAAGGAAG AGAAAGGGGA TTATTATTTA 
GAAGAGGAAG AAGAAGGAGA TGAGGAACAA AGTCTTGAAA CGGAAGTATC AGATGCAAAG 
AATAAAGCTT TCAGCAAGTT TGGATCTTTT TCTGCCACCT TAGAAAATGG AATCTGCCTC 
TCGATAAGTT ACTATGGATC AAATGGAATG GCACCAGAAG ATAAGGATCC TGATTTAGAA 
ACAATATTGA ATATCCCTTC AGCACTCACT CCAACAGTGG TTCCTGTTAT AGTGACCGTT 
CCTCAAAGCA AAGCTAAAGG GAAAATAAAA GGCAAAGAAA AACCCAAAGA ATCCCTTAAA 
GAAGAAGAAC ACCCAAAAGA AGAAGAGAAA AAGGAAGAAG AAGTAGAACC AGAACCTGTT 
TTACAAGAGA CTTTGGATGT TCCCACCTTC CAGAGCCTAA ATGTGTCTTG CCCCAGTGGG 
CTCCTGTTGA CTTTCATTGG ACAAGAATCT ACAGGTCAAT ATGTTATAGA TGAGGAACCC 
ACCTGGGACA TCATGGTCCG TCAGAGCTAC CCCCAGAGGG TGAAGCACTA TGAGTTCTAT 
AAAACGGTGA TGCCACCCGC AGAGCAGGAG GCTTCAAGGG TTATCACCAG TCAAGGCACT 
GTTGTCAAAT ATATGTTGGA TGGATCCACA CAGATTCTCT TTGCAGATGG TGCTGTGAGC 
AGGAGTCCCA ATTCAGGTCT TATTTGTCCT CCTTCTGAAA TGCCAGCAAC GCCTCACAGT 
GGAGATTTGA TGGACTCTAT TTCTCAGCAG AAATCAGAAA CGATACCATC TGAGATTACC 
AACACAAAGA AAGGAAAAAG TCACAAAAGT CAGTCATCAA TGGCCCATAA GGGTGAAATC 
CATGACCCTC CTCCAGAGGC AGTTCAAACT GTAACTCCTG TGGAGGTTCA CATAGGCACC 
TGGTTTACAA CCACACCTGA AGGAAATCGG ATCGGCACCA AAGGATTAGA AAGAATAGCA 
GACTTGACCC CATTGTTATC CTTTCAGGCC ACAGATCCTG TCAATGGAAC GGTTATGACA 
ACTCGAGAAG ACAAAGTTGT CATAGTTGAA AGGAAAGATG GTACTCGGAT AGTGGATCAT 
GCTGATGGTA CCAGAATCAC AACCTTTTAT CAAGTTTATG AAGATCAAAT TATTCTGCCA 
GATGATCAAG AAACAACCGA GGGTCCTCGG ACTGTCACCA GGCAGGTGAA GTGTATGCGG 
GTAGAAAGCT CACGCTATGC CACTGTTATC GCCAACTGTG AGGACAGTAG CTGCTGTGCC 
ACCTTTGGAG ATGGAACAAC TATTATTGCA AAGCCACAGG GAACATACCA GGTGTTACCT 
CCAAACACAG GCTCTCTTTA TATTGACAAG GATTGTTCAG CTGTGTACTG CCATGAGTCA 
AGCAGTAATA TATACTATCC TTTTCAAAAG CGTGAGCAGC TGCGAGCTGG CAGGTACATC 
ATGAGGCATA CTTCAGAGGT TATCTGTGAG GTTCTGGATC CTGAGGGAAA CACTTTTCAG 
GTCATGGCTG ATGGTAGCAT ATCAACTATA TTACCTGAAA AAAAATTGGA AGATGATTTA 
AATGAGAAAA CTGAGGGCTA TGATAGTCTG TCCTCTATGC ACCTTGAAAA GAATCATCAG 
CAAATCTATG GTGAACATGT CCCCAGGTTT TTTGTTATGT ATGCTGATGG ATCAGGAATG 
GAACTTCTTC GAGACAGTGA CATAGAAGAA TATCTATCTT TGGCATATAA AGAATCAAAT 
ACTGTTGTTC TCCAAGAGCC AGTGCAGGAA CAGCCAGGCA CCCTAACCAT CACAGTCCTT 
CGCCCTTTCC ATGAAGCATC ACCATGGCAA GTAAAAAAGG AAGATACAAT TGTCCCTCCT 
AATCTCCGGT CAAGGTCATG GGAAACATTT CCCTCAGTTG AGAAAAAAAC TCCAGGACCT 
CCGTTTGGTA CTCAGATTTG GAAAGGCCTT TGCATTGAGT CCAAACAGCT AGTGAGTGCC 
CCGGGTGCCA TACTCAAGAG CCCCAGTGTG CTACAGATGC GCCAATTCAT TCAGCATGAG 
GTCATAAAGA ATGAGGTGAA ACTGAGGCTG CAGGTTTCCC TTAAGGATTA CATAAACTAT 
ATTCTAAAGA AAGAAGATGA GCTGCAGGAA ATGATGGTTA AAGATTCCAG AACTGAGGAG 
GAGAGAGGCA ATGCTGCTGA TCTCCTCAAG CTGGTTATGT CTTTCCCTAA AATGGAGGAA 
ACTACAAAAA GTCATGTTAC TGAAGTTGCA GCTCACCTAA CTGATTTATT CAAGCAGTCT 
TTGGCTACGC CTCCAAAATG CCCACCAGAC ACATTTGGTA AAGATTTCTT TGAAAAGACA 
TGGAGACACA CAGCATCCTC AAAACGCTGG AAAGAAAAGA TAGACAAAAC GAGGAAGGAA 
ATTGAGACAA CACAGAATTA CCTAATGGAT ATTAAGAACC GCATAATACC ACCCTTTTTT 
AAATCTGAAT TGAACCAGTT ATATCAGTCT CAGTATAATC ACCTGGACAG TCTTTCCAAA 
AAACTGCCTT CTTTTACAAA GAAAAATGAA GATGCAAACG AAACAGCTGT TCAAGATACA 
TCTGATCTTA ATCTAGATTT CAAGCCACAT AAGGTTTCAG AACAGAAATC CTCAAGTGTG 
CCTAGTCTTC CAAAACCAGA GATTTCTGCA GATAAGAAGG ATTTCACTGC TCAGAACCAA 
ACTGAAAATT TAACAAAATC TCCTGAAGAA GCAGAATCTT ATGAGCCCGT GAAAATTCCA 
ACCCAGTCCT TGCTGCAGGA TGTTGCGGGA CAAACAAGAA AAGAAAAAGT GAAGTTGCCT 
CATTATTTGC TGAGTTCCAA GCCTAAGTCT CAACCTCTTG CAAAGGTGCA AGATTCTGTT 
GGAGGAAAAG TGAACACATC CTCTGTTGCA TCTGCTGCCA TTAATAATGC AAAGTCATCC 
CTTTTTGGGT TCCATCTTCT CCCATCATCA GTCAAGTTTG GAGTGCTTAA GGAAGGACAT 
ACCTATGCCA CAGTTGTAAA GCTCAAGAAT GTTGGAGTGG ACTTCTGCAG GTTTAAAGTA 
AAGCAGCCCC CACCCAGCAC AGGACTGAAA GTGACTTACA AACCTGGACC TGTGGCAGCT 
GGTATGCAGA CAGAACTGAA TATAGAGTTA TTTGCCACAG CTGTTGGAGA GGATGGGGCC 
AAGGGATCAG CACACATCTC TCACAATATC GAGATTATGA CAGAGCATGA GGTTCTGTTC 
CTACCTGTGG AAGCAACTGT TTTAACAAGC AGCAATTATG ATAAACGACC AAAAGACTTT 
CCCCAGGGAA AAGAAAATCC AATGGTCCAG AGAACTTCTA CAATTTATTC CTCCACACTT 
GGAGTCTTCA TGTCTCGTAA AGTTTCTCCA CATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006710492.1
CDS	368..4423
Translation	MNEKIIRTRAELELKSSANAKLTSASKIFSIKESKSNKGISKTEISDQEKEKEKEKIPFILEGSLKAWKEEQHRLAEEERLREEKKAEKKGKEAGKKKGKDNAEKEDSRSLKKKSPYKEKSKEEQVKIQEVTEESPHQPEPKITYPFHGYNMGNIPTQISGSNYYLYPSDGGQIEVEKTMFEKGPTFIKVRVVKDNHNFMIHLNDPKEIVKKEEKGDYYLEEEEEGDEEQSLETEVSDAKNKAFSKFGSFSATLENGICLSISYYGSNGMAPEDKDPDLETILNIPSALTPTVVPVIVTVPQSKAKGKIKGKEKPKESLKEEEHPKEEEKKEEEVEPEPVLQETLDVPTFQSLNVSCPSGLLLTFIGQESTGQYVIDEEPTWDIMVRQSYPQRVKHYEFYKTVMPPAEQEASRVITSQGTVVKYMLDGSTQILFADGAVSRSPNSGLICPPSEMPATPHSGDLMDSISQQKSETIPSEITNTKKGKSHKSQSSMAHKGEIHDPPPEAVQTVTPVEVHIGTWFTTTPEGNRIGTKGLERIADLTPLLSFQATDPVNGTVMTTREDKVVIVERKDGTRIVDHADGTRITTFYQVYEDQIILPDDQETTEGPRTVTRQVKCMRVESSRYATVIANCEDSSCCATFGDGTTIIAKPQGTYQVLPPNTGSLYIDKDCSAVYCHESSSNIYYPFQKREQLRAGRYIMRHTSEVICEVLDPEGNTFQVMADGSISTILPEKKLEDDLNEKTEGYDSLSSMHLEKNHQQIYGEHVPRFFVMYADGSGMELLRDSDIEEYLSLAYKESNTVVLQEPVQEQPGTLTITVLRPFHEASPWQVKKEDTIVPPNLRSRSWETFPSVEKKTPGPPFGTQIWKGLCIESKQLVSAPGAILKSPSVLQMRQFIQHEVIKNEVKLRLQVSLKDYINYILKKEDELQEMMVKDSRTEEERGNAADLLKLVMSFPKMEETTKSHVTEVAAHLTDLFKQSLATPPKCPPDTFGKDFFEKTWRHTASSKRWKEKIDKTRKEIETTQNYLMDIKNRIIPPFFKSELNQLYQSQYNHLDSLSKKLPSFTKKNEDANETAVQDTSDLNLDFKPHKVSEQKSSSVPSLPKPEISADKKDFTAQNQTENLTKSPEEAESYEPVKIPTQSLLQDVAGQTRKEKVKLPHYLLSSKPKSQPLAKVQDSVGGKVNTSSVASAAINNAKSSLFGFHLLPSSVKFGVLKEGHTYATVVKLKNVGVDFCRFKVKQPPPSTGLKVTYKPGPVAAGMQTELNIELFATAVGEDGAKGSAHISHNIEIMTEHEVLFLPVEATVLTSSNYDKRPKDFPQGKENPMVQRTSTIYSSTLGVFMSRKVSPH

Target ORF information:

RefSeq Version	XM_006710429.3
Organism	Homo sapiens(human)
Definition	Homo sapiens sperm associated antigen 17 (SPAG17), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006710429.3

ORF Insert Sequence:

ATGAATGAGA AAATCATCAG GACCAGAGCT GAGCTGGAAT TGAAATCTTC TGCTAATGCC 
AAACTTACTT CTGCTAGCAA AATTTTTTCC ATTAAAGAAT CTAAAAGTAA CAAAGGAATC 
AGCAAAACAG AGATATCAGA TCAAGAAAAA GAAAAAGAGA AGGAAAAGAT TCCTTTCATT 
TTAGAAGGCT CTCTCAAGGC ATGGAAAGAA GAGCAACATC GATTAGCAGA AGAGGAGCGC 
TTAAGGGAAG AAAAGAAAGC AGAGAAGAAG GGTAAAGAAG CTGGTAAAAA GAAAGGCAAG 
GATAACGCAG AGAAAGAGGA TAGTAGGTCT TTGAAGAAAA AATCACCTTA CAAGGAGAAA 
TCTAAAGAAG AACAAGTCAA GATCCAAGAA GTAACAGAAG AGTCCCCCCA CCAACCAGAA 
CCTAAGATAA CTTACCCGTT TCACGGATAC AATATGGGAA ATATACCCAC TCAAATCTCA 
GGGTCAAATT ACTACCTGTA TCCTTCTGAT GGGGGGCAGA TTGAAGTGGA AAAGACAATG 
TTTGAAAAAG GCCCAACTTT TATCAAAGTG AGAGTGGTAA AGGACAACCA CAATTTTATG 
ATTCATTTAA ATGACCCTAA GGAAATTGTG AAAAAGGAAG AGAAAGGGGA TTATTATTTA 
GAAGAGGAAG AAGAAGGAGA TGAGGAACAA AGTCTTGAAA CGGAAGTATC AGATGCAAAG 
AATAAAGCTT TCAGCAAGTT TGGATCTTTT TCTGCCACCT TAGAAAATGG AATCTGCCTC 
TCGATAAGTT ACTATGGATC AAATGGAATG GCACCAGAAG ATAAGGATCC TGATTTAGAA 
ACAATATTGA ATATCCCTTC AGCACTCACT CCAACAGTGG TTCCTGTTAT AGTGACCGTT 
CCTCAAAGCA AAGCTAAAGG GAAAATAAAA GGCAAAGAAA AACCCAAAGA ATCCCTTAAA 
GAAGAAGAAC ACCCAAAAGA AGAAGAGAAA AAGGAAGAAG AAGTAGAACC AGAACCTGTT 
TTACAAGAGA CTTTGGATGT TCCCACCTTC CAGAGCCTAA ATGTGTCTTG CCCCAGTGGG 
CTCCTGTTGA CTTTCATTGG ACAAGAATCT ACAGGTCAAT ATGTTATAGA TGAGGAACCC 
ACCTGGGACA TCATGGTCCG TCAGAGCTAC CCCCAGAGGG TGAAGCACTA TGAGTTCTAT 
AAAACGGTGA TGCCACCCGC AGAGCAGGAG GCTTCAAGGG TTATCACCAG TCAAGGCACT 
GTTGTCAAAT ATATGTTGGA TGGATCCACA CAGATTCTCT TTGCAGATGG TGCTGTGAGC 
AGGAGTCCCA ATTCAGGTCT TATTTGTCCT CCTTCTGAAA TGCCAGCAAC GCCTCACAGT 
GGAGATTTGA TGGACTCTAT TTCTCAGCAG AAATCAGAAA CGATACCATC TGAGATTACC 
AACACAAAGA AAGGAAAAAG TCACAAAAGT CAGTCATCAA TGGCCCATAA GGGTGAAATC 
CATGACCCTC CTCCAGAGGC AGTTCAAACT GTAACTCCTG TGGAGGTTCA CATAGGCACC 
TGGTTTACAA CCACACCTGA AGGAAATCGG ATCGGCACCA AAGGATTAGA AAGAATAGCA 
GACTTGACCC CATTGTTATC CTTTCAGGCC ACAGATCCTG TCAATGGAAC GGTTATGACA 
ACTCGAGAAG ACAAAGTTGT CATAGTTGAA AGGAAAGATG GTACTCGGAT AGTGGATCAT 
GCTGATGGTA CCAGAATCAC AACCTTTTAT CAAGTTTATG AAGATCAAAT TATTCTGCCA 
GATGATCAAG AAACAACCGA GGGTCCTCGG ACTGTCACCA GGCAGGTGAA GTGTATGCGG 
GTAGAAAGCT CACGCTATGC CACTGTTATC GCCAACTGTG AGGACAGTAG CTGCTGTGCC 
ACCTTTGGAG ATGGAACAAC TATTATTGCA AAGCCACAGG GAACATACCA GGTGTTACCT 
CCAAACACAG GCTCTCTTTA TATTGACAAG GATTGTTCAG CTGTGTACTG CCATGAGTCA 
AGCAGTAATA TATACTATCC TTTTCAAAAG CGTGAGCAGC TGCGAGCTGG CAGGTACATC 
ATGAGGCATA CTTCAGAGGT TATCTGTGAG GTTCTGGATC CTGAGGGAAA CACTTTTCAG 
GTCATGGCTG ATGGTAGCAT ATCAACTATA TTACCTGAAA AAAAATTGGA AGATGATTTA 
AATGAGAAAA CTGAGGGCTA TGATAGTCTG TCCTCTATGC ACCTTGAAAA GAATCATCAG 
CAAATCTATG GTGAACATGT CCCCAGGTTT TTTGTTATGT ATGCTGATGG ATCAGGAATG 
GAACTTCTTC GAGACAGTGA CATAGAAGAA TATCTATCTT TGGCATATAA AGAATCAAAT 
ACTGTTGTTC TCCAAGAGCC AGTGCAGGAA CAGCCAGGCA CCCTAACCAT CACAGTCCTT 
CGCCCTTTCC ATGAAGCATC ACCATGGCAA GTAAAAAAGG AAGATACAAT TGTCCCTCCT 
AATCTCCGGT CAAGGTCATG GGAAACATTT CCCTCAGTTG AGAAAAAAAC TCCAGGACCT 
CCGTTTGGTA CTCAGATTTG GAAAGGCCTT TGCATTGAGT CCAAACAGCT AGTGAGTGCC 
CCGGGTGCCA TACTCAAGAG CCCCAGTGTG CTACAGATGC GCCAATTCAT TCAGCATGAG 
GTCATAAAGA ATGAGGTGAA ACTGAGGCTG CAGGTTTCCC TTAAGGATTA CATAAACTAT 
ATTCTAAAGA AAGAAGATGA GCTGCAGGAA ATGATGGTTA AAGATTCCAG AACTGAGGAG 
GAGAGAGGCA ATGCTGCTGA TCTCCTCAAG CTGGTTATGT CTTTCCCTAA AATGGAGGAA 
ACTACAAAAA GTCATGTTAC TGAAGTTGCA GCTCACCTAA CTGATTTATT CAAGCAGTCT 
TTGGCTACGC CTCCAAAATG CCCACCAGAC ACATTTGGTA AAGATTTCTT TGAAAAGACA 
TGGAGACACA CAGCATCCTC AAAACGCTGG AAAGAAAAGA TAGACAAAAC GAGGAAGGAA 
ATTGAGACAA CACAGAATTA CCTAATGGAT ATTAAGAACC GCATAATACC ACCCTTTTTT 
AAATCTGAAT TGAACCAGTT ATATCAGTCT CAGTATAATC ACCTGGACAG TCTTTCCAAA 
AAACTGCCTT CTTTTACAAA GAAAAATGAA GATGCAAACG AAACAGCTGT TCAAGATACA 
TCTGATCTTA ATCTAGATTT CAAGCCACAT AAGGTTTCAG AACAGAAATC CTCAAGTGTG 
CCTAGTCTTC CAAAACCAGA GATTTCTGCA GATAAGAAGG ATTTCACTGC TCAGAACCAA 
ACTGAAAATT TAACAAAATC TCCTGAAGAA GCAGAATCTT ATGAGCCCGT GAAAATTCCA 
ACCCAGTCCT TGCTGCAGGA TGTTGCGGGA CAAACAAGAA AAGAAAAAGT GAAGTTGCCT 
CATTATTTGC TGAGTTCCAA GCCTAAGTCT CAACCTCTTG CAAAGGTGCA AGATTCTGTT 
GGAGGAAAAG TGAACACATC CTCTGTTGCA TCTGCTGCCA TTAATAATGC AAAGTCATCC 
CTTTTTGGGT TCCATCTTCT CCCATCATCA GTCAAGTTTG GAGTGCTTAA GGAAGGACAT 
ACCTATGCCA CAGTTGTAAA GCTCAAGAAT GTTGGAGTGG ACTTCTGCAG GTTTAAAGTA 
AAGCAGCCCC CACCCAGCAC AGGACTGAAA GTGACTTACA AACCTGGACC TGTGGCAGCT 
GGTATGCAGA CAGAACTGAA TATAGAGTTA TTTGCCACAG CTGTTGGAGA GGATGGGGCC 
AAGGGATCAG CACACATCTC TCACAATATC GAGATTATGA CAGAGCATGA GGTTCTGTTC 
CTACCTGTGG AAGCAACTGT TTTAACAAGC AGCAATTATG ATAAACGACC AAAAGACTTT 
CCCCAGGGAA AAGAAAATCC AATGGTCCAG AGAACTTCTA CAATTTATTC CTCCACACTT 
GGAGTCTTCA TGTCTCGTAA AGTTTCTCCA CATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPAG17 ( XM_011540936.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011539238.1 Homo sapiens sperm associated antigen 17 (SPAG17), transcript variant X6, mRNA.

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CloneID	OHu77636
Clone ID Related Accession (Same CDS sequence)	XM_011540936.2 , XM_011540937.2
Accession Version	XM_011540936.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5022bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	sperm-associated antigen 17 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011540936.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGTCCA AGTTGCCACT GTGGGAATTT CTTCAATTCC CTCTACCCCC ACCATGGAAC 
AACACTAAAC GTCTAGCTAC AATTCATGAG CTTATGCACT TTTGTACGAG TGATGTCTTG 
AGCTGGAATG AAGTAGAACG AGCCTTCAAG GTGTTTACTT TTGAGAGCCT GAAGCTCTCT 
GAGGTTGATG AAAAAGGGAA ACTGAAACCT TCTGGGATGA TGTGTGGGTC AGATTCTGAA 
ATGTTCAACA TACCGTGGGA CAACCCTGCC AGATTTGCTA AACAGATAAG GCAGCAATAT 
GTCATGAAAA TGAATACTCA AGAGGCCAAG CAGAAAGCAG ATATTAAAAT CAAAGACAGA 
ACACTATTTG TGGATCAGAA TTTGTCAATG TCTGTGCAAG ATAATGAAAG CAACCGAGAA 
CCTTCAGATC CTAGTCAGTG TGATGCTAAC AATATGAAGC ATTCTGACTT GAATAATCTC 
AAACTCTCAG TCCCTGATAA TAGACAGCTG TTAGAGCAGG AGAGCATCAT GAAGGCTCAG 
CCCCAACATG AGTCTCTGGA GCAGACCACA AACAATGAGA TCAAAGATGA TGCAGTCACA 
AAGGCTGATT CTCATGAAAA GAAACCCAAG AAGATGATGG TGGAAGCAGA TTTAGAGGAC 
ATAAAGAAAA CACAGCAGCG CAGTCTAATG GACTGGAGTT TTACTGAACA TTTTAAACCG 
AAAGTACTGC TTCAGGTCCT TCAAGAAGCC CATAAGCAAT ATAGGTGTGT TGATTCTTAC 
TACCACACCC AAGACAACTC TTTACTTTTA GTCTTTCACA ATCCAATGAA TAGACAACGT 
TTGCATTGTG AATATTGGAA CATTGCTCTC CACTCCAATG TTGGATTCAG GAATTATTTG 
GAACTTGTTG CAAAATCTAT TCAAGATTGG ATTACAAAAG AAGAAGCTAT ATATCAGGAA 
TCTAAAATGA ATGAGAAAAT CATCAGGACC AGAGCTGAGC TGGAATTGAA ATCTTCTGCT 
AATGCCAAAC TTACTTCTGC TAGCAAAATT TTTTCCATTA AAGAATCTAA AAGTAACAAA 
GGAATCAGCA AAACAGAGAT ATCAGATCAA GAAAAAGAAA AAGAGAAGGA AAAGATTCCT 
TTCATTTTAG AAGGCTCTCT CAAGGCATGG AAAGAAGAGC AACATCGATT AGCAGAAGAG 
GAGCGCTTAA GGGAAGAAAA GAAAGCAGAG AAGAAGGGTA AAGAAGCTGG TAAAAAGAAA 
GGCAAGGATA ACGCAGAGAA AGAGGATAGT AGGTCTTTGA AGAAAAAATC ACCTTACAAG 
GAGAAATCTA AAGAAGAACA AGTCAAGATC CAAGAAGTAA CAGAAGAGTC CCCCCACCAA 
CCAGAACCTA AGATAACTTA CCCGTTTCAC GGATACAATA TGGGAAATAT ACCCACTCAA 
ATCTCAGGGT CAAATTACTA CCTGTATCCT TCTGATGGGG GGCAGATTGA AGTGGAAAAG 
ACAATGTTTG AAAAAGGCCC AACTTTTATC AAAGTGAGAG TGGTAAAGGA CAACCACAAT 
TTTATGATTC ATTTAAATGA CCCTAAGGAA ATTGTGAAAA AGGAAGAGAA AGGGGATTAT 
TATTTAGAAG AGGAAGAAGA AGGAGATGAG GAACAAAGTC TTGAAACGGA AGTATCAGAT 
GCAAAGAATA AAGCTTTCAG CAAGTTTGGA TCTTTTTCTG CCACCTTAGA AAATGGAATC 
TGCCTCTCGA TAAGTTACTA TGGATCAAAT GGAATGGCAC CAGAAGATAA GGATCCTGAT 
TTAGAAACAA TATTGAATAT CCCTTCAGCA CTCACTCCAA CAGTGGTTCC TGTTATAGTG 
ACCGTTCCTC AAAGCAAAGC TAAAGGGAAA ATAAAAGGCA AAGAAAAACC CAAAGAATCC 
CTTAAAGAAG AAGAACACCC AAAAGAAGAA GAGAAAAAGG AAGAAGAAGT AGAACCAGAA 
CCTGTTTTAC AAGAGACTTT GGATGTTCCC ACCTTCCAGA GCCTAAATGT GTCTTGCCCC 
AGTGGGCTCC TGTTGACTTT CATTGGACAA GAATCTACAG GTCAATATGT TATAGATGAG 
GAACCCACCT GGGACATCAT GGTCCGTCAG AGCTACCCCC AGAGGGTGAA GCACTATGAG 
TTCTATAAAA CGGTGATGCC ACCCGCAGAG CAGGAGGCTT CAAGGGTTAT CACCAGTCAA 
GGCACTGTTG TCAAATATAT GTTGGATGGA TCCACACAGA TTCTCTTTGC AGATGGTGCT 
GTGAGCAGGA GTCCCAATTC AGGTCTTATT TGTCCTCCTT CTGAAATGCC AGCAACGCCT 
CACAGTGGAG ATTTGATGGA CTCTATTTCT CAGCAGAAAT CAGAAACGAT ACCATCTGAG 
ATTACCAACA CAAAGAAAGG AAAAAGTCAC AAAAGTCAGT CATCAATGGC CCATAAGGGT 
GAAATCCATG ACCCTCCTCC AGAGGCAGTT CAAACTGTAA CTCCTGTGGA GGTTCACATA 
GGCACCTGGT TTACAACCAC ACCTGAAGGA AATCGGATCG GCACCAAAGG ATTAGAAAGA 
ATAGCAGACT TGACCCCATT GTTATCCTTT CAGGCCACAG ATCCTGTCAA TGGAACGGTT 
ATGACAACTC GAGAAGACAA AGTTGTCATA GTTGAAAGGA AAGATGGTAC TCGGATAGTG 
GATCATGCTG ATGGTACCAG AATCACAACC TTTTATCAAG TTTATGAAGA TCAAATTATT 
CTGCCAGATG ATCAAGAAAC AACCGAGGGT CCTCGGACTG TCACCAGGCA GGTGAAGTGT 
ATGCGGGTAG AAAGCTCACG CTATGCCACT GTTATCGCCA ACTGTGAGGA CAGTAGCTGC 
TGTGCCACCT TTGGAGATGG AACAACTATT ATTGCAAAGC CACAGGGAAC ATACCAGGTG 
TTACCTCCAA ACACAGGCTC TCTTTATATT GACAAGGATT GTTCAGCTGT GTACTGCCAT 
GAGTCAAGCA GTAATATATA CTATCCTTTT CAAAAGCGTG AGCAGCTGCG AGCTGGCAGG 
TACATCATGA GGCATACTTC AGAGGTTATC TGTGAGGTTC TGGATCCTGA GGGAAACACT 
TTTCAGGTCA TGGCTGATGG TAGCATATCA ACTATATTAC CTGAAAAAAA ATTGGAAGAT 
GATTTAAATG AGAAAACTGA GGGCTATGAT AGTCTGTCCT CTATGCACCT TGAAAAGAAT 
CATCAGCAAA TCTATGGTGA ACATGTCCCC AGGTTTTTTG TTATGTATGC TGATGGATCA 
GGAATGGAAC TTCTTCGAGA CAGTGACATA GAAGAATATC TATCTTTGGC ATATAAAGAA 
TCAAATACTG TTGTTCTCCA AGAGCCAGTG CAGGAACAGC CAGGCACCCT AACCATCACA 
GTCCTTCGCC CTTTCCATGA AGCATCACCA TGGCAAGTAA AAAAGGAAGA TACAATTGTC 
CCTCCTAATC TCCGGTCAAG GTCATGGGAA ACATTTCCCT CAGTTGAGAA AAAAACTCCA 
GGACCTCCGT TTGGTACTCA GATTTGGAAA GGCCTTTGCA TTGAGTCCAA ACAGCTAGTG 
AGTGCCCCGG GTGCCATACT CAAGAGCCCC AGTGTGCTAC AGATGCGCCA ATTCATTCAG 
CATGAGGTCA TAAAGAATGA GGTGAAACTG AGGCTGCAGG TTTCCCTTAA GGATTACATA 
AACTATATTC TAAAGAAAGA AGATGAGCTG CAGGAAATGA TGGTTAAAGA TTCCAGAACT 
GAGGAGGAGA GAGGCAATGC TGCTGATCTC CTCAAGCTGG TTATGTCTTT CCCTAAAATG 
GAGGAAACTA CAAAAAGTCA TGTTACTGAA GTTGCAGCTC ACCTAACTGA TTTATTCAAG 
CAGTCTTTGG CTACGCCTCC AAAATGCCCA CCAGACACAT TTGGTAAAGA TTTCTTTGAA 
AAGACATGGA GACACACAGC ATCCTCAAAA CGCTGGAAAG AAAAGATAGA CAAAACGAGG 
AAGGAAATTG AGACAACACA GAATTACCTA ATGGATATTA AGAACCGCAT AATACCACCC 
TTTTTTAAAT CTGAATTGAA CCAGTTATAT CAGTCTCAGT ATAATCACCT GGACAGTCTT 
TCCAAAAAAC TGCCTTCTTT TACAAAGAAA AATGAAGATG CAAACGAAAC AGCTGTTCAA 
GATACATCTG ATCTTAATCT AGATTTCAAG CCACATAAGG TTTCAGAACA GAAATCCTCA 
AGTGTGCCTA GTCTTCCAAA ACCAGAGATT TCTGCAGATA AGAAGGATTT CACTGCTCAG 
AACCAAACTG AAAATTTAAC AAAATCTCCT GAAGAAGCAG AATCTTATGA GCCCGTGAAA 
ATTCCAACCC AGTCCTTGCT GCAGGATGTT GCGGGACAAA CAAGAAAAGA AAAAGTGAAG 
TTGCCTCATT ATTTGCTGAG TTCCAAGCCT AAGTCTCAAC CTCTTGCAAA GGTGCAAGAT 
TCTGTTGGAG GAAAAGTGAA CACATCCTCT GTTGCATCTG CTGCCATTAA TAATGCAAAG 
TCATCCCTTT TTGGGTTCCA TCTTCTCCCA TCATCAGTCA AGTTTGGAGT GCTTAAGGAA 
GGACATACCT ATGCCACAGT TGTAAAGCTC AAGAATGTTG GAGTGGACTT CTGCAGGTTT 
AAAGTAAAGC AGCCCCCACC CAGCACAGGA CTGAAAGTGA CTTACAAACC TGGACCTGTG 
GCAGCTGGTA TGCAGACAGA ACTGAATATA GAGTTATTTG CCACAGCTGT TGGAGAGGAT 
GGGGCCAAGG GATCAGCACA CATCTCTCAC AATATCGAGA TTATGACAGA GCATGAGGTT 
CTGTTCCTAC CTGTGGAAGC AACTGTTTTA ACAAGCAGCA ATTATGATAA ACGACCAAAA 
GACTTTCCCC AGGGAAAAGA AAATCCAATG GTCCAGAGAA CTTCTACAAT TTATTCCTCC 
ACACTTGGAG TCTTCATGTC TCGTAAAGTT TCTCCACATT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011539238.1
CDS	377..5398
Translation	MQSKLPLWEFLQFPLPPPWNNTKRLATIHELMHFCTSDVLSWNEVERAFKVFTFESLKLSEVDEKGKLKPSGMMCGSDSEMFNIPWDNPARFAKQIRQQYVMKMNTQEAKQKADIKIKDRTLFVDQNLSMSVQDNESNREPSDPSQCDANNMKHSDLNNLKLSVPDNRQLLEQESIMKAQPQHESLEQTTNNEIKDDAVTKADSHEKKPKKMMVEADLEDIKKTQQRSLMDWSFTEHFKPKVLLQVLQEAHKQYRCVDSYYHTQDNSLLLVFHNPMNRQRLHCEYWNIALHSNVGFRNYLELVAKSIQDWITKEEAIYQESKMNEKIIRTRAELELKSSANAKLTSASKIFSIKESKSNKGISKTEISDQEKEKEKEKIPFILEGSLKAWKEEQHRLAEEERLREEKKAEKKGKEAGKKKGKDNAEKEDSRSLKKKSPYKEKSKEEQVKIQEVTEESPHQPEPKITYPFHGYNMGNIPTQISGSNYYLYPSDGGQIEVEKTMFEKGPTFIKVRVVKDNHNFMIHLNDPKEIVKKEEKGDYYLEEEEEGDEEQSLETEVSDAKNKAFSKFGSFSATLENGICLSISYYGSNGMAPEDKDPDLETILNIPSALTPTVVPVIVTVPQSKAKGKIKGKEKPKESLKEEEHPKEEEKKEEEVEPEPVLQETLDVPTFQSLNVSCPSGLLLTFIGQESTGQYVIDEEPTWDIMVRQSYPQRVKHYEFYKTVMPPAEQEASRVITSQGTVVKYMLDGSTQILFADGAVSRSPNSGLICPPSEMPATPHSGDLMDSISQQKSETIPSEITNTKKGKSHKSQSSMAHKGEIHDPPPEAVQTVTPVEVHIGTWFTTTPEGNRIGTKGLERIADLTPLLSFQATDPVNGTVMTTREDKVVIVERKDGTRIVDHADGTRITTFYQVYEDQIILPDDQETTEGPRTVTRQVKCMRVESSRYATVIANCEDSSCCATFGDGTTIIAKPQGTYQVLPPNTGSLYIDKDCSAVYCHESSSNIYYPFQKREQLRAGRYIMRHTSEVICEVLDPEGNTFQVMADGSISTILPEKKLEDDLNEKTEGYDSLSSMHLEKNHQQIYGEHVPRFFVMYADGSGMELLRDSDIEEYLSLAYKESNTVVLQEPVQEQPGTLTITVLRPFHEASPWQVKKEDTIVPPNLRSRSWETFPSVEKKTPGPPFGTQIWKGLCIESKQLVSAPGAILKSPSVLQMRQFIQHEVIKNEVKLRLQVSLKDYINYILKKEDELQEMMVKDSRTEEERGNAADLLKLVMSFPKMEETTKSHVTEVAAHLTDLFKQSLATPPKCPPDTFGKDFFEKTWRHTASSKRWKEKIDKTRKEIETTQNYLMDIKNRIIPPFFKSELNQLYQSQYNHLDSLSKKLPSFTKKNEDANETAVQDTSDLNLDFKPHKVSEQKSSSVPSLPKPEISADKKDFTAQNQTENLTKSPEEAESYEPVKIPTQSLLQDVAGQTRKEKVKLPHYLLSSKPKSQPLAKVQDSVGGKVNTSSVASAAINNAKSSLFGFHLLPSSVKFGVLKEGHTYATVVKLKNVGVDFCRFKVKQPPPSTGLKVTYKPGPVAAGMQTELNIELFATAVGEDGAKGSAHISHNIEIMTEHEVLFLPVEATVLTSSNYDKRPKDFPQGKENPMVQRTSTIYSSTLGVFMSRKVSPH

Target ORF information:

RefSeq Version	XM_011540936.2
Organism	Homo sapiens(human)
Definition	Homo sapiens sperm associated antigen 17 (SPAG17), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011540936.2

ORF Insert Sequence:

ATGCAGTCCA AGTTGCCACT GTGGGAATTT CTTCAATTCC CTCTACCCCC ACCATGGAAC 
AACACTAAAC GTCTAGCTAC AATTCATGAG CTTATGCACT TTTGTACGAG TGATGTCTTG 
AGCTGGAATG AAGTAGAACG AGCCTTCAAG GTGTTTACTT TTGAGAGCCT GAAGCTCTCT 
GAGGTTGATG AAAAAGGGAA ACTGAAACCT TCTGGGATGA TGTGTGGGTC AGATTCTGAA 
ATGTTCAACA TACCGTGGGA CAACCCTGCC AGATTTGCTA AACAGATAAG GCAGCAATAT 
GTCATGAAAA TGAATACTCA AGAGGCCAAG CAGAAAGCAG ATATTAAAAT CAAAGACAGA 
ACACTATTTG TGGATCAGAA TTTGTCAATG TCTGTGCAAG ATAATGAAAG CAACCGAGAA 
CCTTCAGATC CTAGTCAGTG TGATGCTAAC AATATGAAGC ATTCTGACTT GAATAATCTC 
AAACTCTCAG TCCCTGATAA TAGACAGCTG TTAGAGCAGG AGAGCATCAT GAAGGCTCAG 
CCCCAACATG AGTCTCTGGA GCAGACCACA AACAATGAGA TCAAAGATGA TGCAGTCACA 
AAGGCTGATT CTCATGAAAA GAAACCCAAG AAGATGATGG TGGAAGCAGA TTTAGAGGAC 
ATAAAGAAAA CACAGCAGCG CAGTCTAATG GACTGGAGTT TTACTGAACA TTTTAAACCG 
AAAGTACTGC TTCAGGTCCT TCAAGAAGCC CATAAGCAAT ATAGGTGTGT TGATTCTTAC 
TACCACACCC AAGACAACTC TTTACTTTTA GTCTTTCACA ATCCAATGAA TAGACAACGT 
TTGCATTGTG AATATTGGAA CATTGCTCTC CACTCCAATG TTGGATTCAG GAATTATTTG 
GAACTTGTTG CAAAATCTAT TCAAGATTGG ATTACAAAAG AAGAAGCTAT ATATCAGGAA 
TCTAAAATGA ATGAGAAAAT CATCAGGACC AGAGCTGAGC TGGAATTGAA ATCTTCTGCT 
AATGCCAAAC TTACTTCTGC TAGCAAAATT TTTTCCATTA AAGAATCTAA AAGTAACAAA 
GGAATCAGCA AAACAGAGAT ATCAGATCAA GAAAAAGAAA AAGAGAAGGA AAAGATTCCT 
TTCATTTTAG AAGGCTCTCT CAAGGCATGG AAAGAAGAGC AACATCGATT AGCAGAAGAG 
GAGCGCTTAA GGGAAGAAAA GAAAGCAGAG AAGAAGGGTA AAGAAGCTGG TAAAAAGAAA 
GGCAAGGATA ACGCAGAGAA AGAGGATAGT AGGTCTTTGA AGAAAAAATC ACCTTACAAG 
GAGAAATCTA AAGAAGAACA AGTCAAGATC CAAGAAGTAA CAGAAGAGTC CCCCCACCAA 
CCAGAACCTA AGATAACTTA CCCGTTTCAC GGATACAATA TGGGAAATAT ACCCACTCAA 
ATCTCAGGGT CAAATTACTA CCTGTATCCT TCTGATGGGG GGCAGATTGA AGTGGAAAAG 
ACAATGTTTG AAAAAGGCCC AACTTTTATC AAAGTGAGAG TGGTAAAGGA CAACCACAAT 
TTTATGATTC ATTTAAATGA CCCTAAGGAA ATTGTGAAAA AGGAAGAGAA AGGGGATTAT 
TATTTAGAAG AGGAAGAAGA AGGAGATGAG GAACAAAGTC TTGAAACGGA AGTATCAGAT 
GCAAAGAATA AAGCTTTCAG CAAGTTTGGA TCTTTTTCTG CCACCTTAGA AAATGGAATC 
TGCCTCTCGA TAAGTTACTA TGGATCAAAT GGAATGGCAC CAGAAGATAA GGATCCTGAT 
TTAGAAACAA TATTGAATAT CCCTTCAGCA CTCACTCCAA CAGTGGTTCC TGTTATAGTG 
ACCGTTCCTC AAAGCAAAGC TAAAGGGAAA ATAAAAGGCA AAGAAAAACC CAAAGAATCC 
CTTAAAGAAG AAGAACACCC AAAAGAAGAA GAGAAAAAGG AAGAAGAAGT AGAACCAGAA 
CCTGTTTTAC AAGAGACTTT GGATGTTCCC ACCTTCCAGA GCCTAAATGT GTCTTGCCCC 
AGTGGGCTCC TGTTGACTTT CATTGGACAA GAATCTACAG GTCAATATGT TATAGATGAG 
GAACCCACCT GGGACATCAT GGTCCGTCAG AGCTACCCCC AGAGGGTGAA GCACTATGAG 
TTCTATAAAA CGGTGATGCC ACCCGCAGAG CAGGAGGCTT CAAGGGTTAT CACCAGTCAA 
GGCACTGTTG TCAAATATAT GTTGGATGGA TCCACACAGA TTCTCTTTGC AGATGGTGCT 
GTGAGCAGGA GTCCCAATTC AGGTCTTATT TGTCCTCCTT CTGAAATGCC AGCAACGCCT 
CACAGTGGAG ATTTGATGGA CTCTATTTCT CAGCAGAAAT CAGAAACGAT ACCATCTGAG 
ATTACCAACA CAAAGAAAGG AAAAAGTCAC AAAAGTCAGT CATCAATGGC CCATAAGGGT 
GAAATCCATG ACCCTCCTCC AGAGGCAGTT CAAACTGTAA CTCCTGTGGA GGTTCACATA 
GGCACCTGGT TTACAACCAC ACCTGAAGGA AATCGGATCG GCACCAAAGG ATTAGAAAGA 
ATAGCAGACT TGACCCCATT GTTATCCTTT CAGGCCACAG ATCCTGTCAA TGGAACGGTT 
ATGACAACTC GAGAAGACAA AGTTGTCATA GTTGAAAGGA AAGATGGTAC TCGGATAGTG 
GATCATGCTG ATGGTACCAG AATCACAACC TTTTATCAAG TTTATGAAGA TCAAATTATT 
CTGCCAGATG ATCAAGAAAC AACCGAGGGT CCTCGGACTG TCACCAGGCA GGTGAAGTGT 
ATGCGGGTAG AAAGCTCACG CTATGCCACT GTTATCGCCA ACTGTGAGGA CAGTAGCTGC 
TGTGCCACCT TTGGAGATGG AACAACTATT ATTGCAAAGC CACAGGGAAC ATACCAGGTG 
TTACCTCCAA ACACAGGCTC TCTTTATATT GACAAGGATT GTTCAGCTGT GTACTGCCAT 
GAGTCAAGCA GTAATATATA CTATCCTTTT CAAAAGCGTG AGCAGCTGCG AGCTGGCAGG 
TACATCATGA GGCATACTTC AGAGGTTATC TGTGAGGTTC TGGATCCTGA GGGAAACACT 
TTTCAGGTCA TGGCTGATGG TAGCATATCA ACTATATTAC CTGAAAAAAA ATTGGAAGAT 
GATTTAAATG AGAAAACTGA GGGCTATGAT AGTCTGTCCT CTATGCACCT TGAAAAGAAT 
CATCAGCAAA TCTATGGTGA ACATGTCCCC AGGTTTTTTG TTATGTATGC TGATGGATCA 
GGAATGGAAC TTCTTCGAGA CAGTGACATA GAAGAATATC TATCTTTGGC ATATAAAGAA 
TCAAATACTG TTGTTCTCCA AGAGCCAGTG CAGGAACAGC CAGGCACCCT AACCATCACA 
GTCCTTCGCC CTTTCCATGA AGCATCACCA TGGCAAGTAA AAAAGGAAGA TACAATTGTC 
CCTCCTAATC TCCGGTCAAG GTCATGGGAA ACATTTCCCT CAGTTGAGAA AAAAACTCCA 
GGACCTCCGT TTGGTACTCA GATTTGGAAA GGCCTTTGCA TTGAGTCCAA ACAGCTAGTG 
AGTGCCCCGG GTGCCATACT CAAGAGCCCC AGTGTGCTAC AGATGCGCCA ATTCATTCAG 
CATGAGGTCA TAAAGAATGA GGTGAAACTG AGGCTGCAGG TTTCCCTTAA GGATTACATA 
AACTATATTC TAAAGAAAGA AGATGAGCTG CAGGAAATGA TGGTTAAAGA TTCCAGAACT 
GAGGAGGAGA GAGGCAATGC TGCTGATCTC CTCAAGCTGG TTATGTCTTT CCCTAAAATG 
GAGGAAACTA CAAAAAGTCA TGTTACTGAA GTTGCAGCTC ACCTAACTGA TTTATTCAAG 
CAGTCTTTGG CTACGCCTCC AAAATGCCCA CCAGACACAT TTGGTAAAGA TTTCTTTGAA 
AAGACATGGA GACACACAGC ATCCTCAAAA CGCTGGAAAG AAAAGATAGA CAAAACGAGG 
AAGGAAATTG AGACAACACA GAATTACCTA ATGGATATTA AGAACCGCAT AATACCACCC 
TTTTTTAAAT CTGAATTGAA CCAGTTATAT CAGTCTCAGT ATAATCACCT GGACAGTCTT 
TCCAAAAAAC TGCCTTCTTT TACAAAGAAA AATGAAGATG CAAACGAAAC AGCTGTTCAA 
GATACATCTG ATCTTAATCT AGATTTCAAG CCACATAAGG TTTCAGAACA GAAATCCTCA 
AGTGTGCCTA GTCTTCCAAA ACCAGAGATT TCTGCAGATA AGAAGGATTT CACTGCTCAG 
AACCAAACTG AAAATTTAAC AAAATCTCCT GAAGAAGCAG AATCTTATGA GCCCGTGAAA 
ATTCCAACCC AGTCCTTGCT GCAGGATGTT GCGGGACAAA CAAGAAAAGA AAAAGTGAAG 
TTGCCTCATT ATTTGCTGAG TTCCAAGCCT AAGTCTCAAC CTCTTGCAAA GGTGCAAGAT 
TCTGTTGGAG GAAAAGTGAA CACATCCTCT GTTGCATCTG CTGCCATTAA TAATGCAAAG 
TCATCCCTTT TTGGGTTCCA TCTTCTCCCA TCATCAGTCA AGTTTGGAGT GCTTAAGGAA 
GGACATACCT ATGCCACAGT TGTAAAGCTC AAGAATGTTG GAGTGGACTT CTGCAGGTTT 
AAAGTAAAGC AGCCCCCACC CAGCACAGGA CTGAAAGTGA CTTACAAACC TGGACCTGTG 
GCAGCTGGTA TGCAGACAGA ACTGAATATA GAGTTATTTG CCACAGCTGT TGGAGAGGAT 
GGGGCCAAGG GATCAGCACA CATCTCTCAC AATATCGAGA TTATGACAGA GCATGAGGTT 
CTGTTCCTAC CTGTGGAAGC AACTGTTTTA ACAAGCAGCA ATTATGATAA ACGACCAAAA 
GACTTTCCCC AGGGAAAAGA AAATCCAATG GTCCAGAGAA CTTCTACAAT TTATTCCTCC 
ACACTTGGAG TCTTCATGTC TCGTAAAGTT TCTCCACATT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPAG17 ( XM_011540937.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011539239.1 Homo sapiens sperm associated antigen 17 (SPAG17), transcript variant X7, mRNA.

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CloneID	OHu77636
Clone ID Related Accession (Same CDS sequence)	XM_011540936.2 , XM_011540937.2
Accession Version	XM_011540937.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5022bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	sperm-associated antigen 17 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011540937.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGTCCA AGTTGCCACT GTGGGAATTT CTTCAATTCC CTCTACCCCC ACCATGGAAC 
AACACTAAAC GTCTAGCTAC AATTCATGAG CTTATGCACT TTTGTACGAG TGATGTCTTG 
AGCTGGAATG AAGTAGAACG AGCCTTCAAG GTGTTTACTT TTGAGAGCCT GAAGCTCTCT 
GAGGTTGATG AAAAAGGGAA ACTGAAACCT TCTGGGATGA TGTGTGGGTC AGATTCTGAA 
ATGTTCAACA TACCGTGGGA CAACCCTGCC AGATTTGCTA AACAGATAAG GCAGCAATAT 
GTCATGAAAA TGAATACTCA AGAGGCCAAG CAGAAAGCAG ATATTAAAAT CAAAGACAGA 
ACACTATTTG TGGATCAGAA TTTGTCAATG TCTGTGCAAG ATAATGAAAG CAACCGAGAA 
CCTTCAGATC CTAGTCAGTG TGATGCTAAC AATATGAAGC ATTCTGACTT GAATAATCTC 
AAACTCTCAG TCCCTGATAA TAGACAGCTG TTAGAGCAGG AGAGCATCAT GAAGGCTCAG 
CCCCAACATG AGTCTCTGGA GCAGACCACA AACAATGAGA TCAAAGATGA TGCAGTCACA 
AAGGCTGATT CTCATGAAAA GAAACCCAAG AAGATGATGG TGGAAGCAGA TTTAGAGGAC 
ATAAAGAAAA CACAGCAGCG CAGTCTAATG GACTGGAGTT TTACTGAACA TTTTAAACCG 
AAAGTACTGC TTCAGGTCCT TCAAGAAGCC CATAAGCAAT ATAGGTGTGT TGATTCTTAC 
TACCACACCC AAGACAACTC TTTACTTTTA GTCTTTCACA ATCCAATGAA TAGACAACGT 
TTGCATTGTG AATATTGGAA CATTGCTCTC CACTCCAATG TTGGATTCAG GAATTATTTG 
GAACTTGTTG CAAAATCTAT TCAAGATTGG ATTACAAAAG AAGAAGCTAT ATATCAGGAA 
TCTAAAATGA ATGAGAAAAT CATCAGGACC AGAGCTGAGC TGGAATTGAA ATCTTCTGCT 
AATGCCAAAC TTACTTCTGC TAGCAAAATT TTTTCCATTA AAGAATCTAA AAGTAACAAA 
GGAATCAGCA AAACAGAGAT ATCAGATCAA GAAAAAGAAA AAGAGAAGGA AAAGATTCCT 
TTCATTTTAG AAGGCTCTCT CAAGGCATGG AAAGAAGAGC AACATCGATT AGCAGAAGAG 
GAGCGCTTAA GGGAAGAAAA GAAAGCAGAG AAGAAGGGTA AAGAAGCTGG TAAAAAGAAA 
GGCAAGGATA ACGCAGAGAA AGAGGATAGT AGGTCTTTGA AGAAAAAATC ACCTTACAAG 
GAGAAATCTA AAGAAGAACA AGTCAAGATC CAAGAAGTAA CAGAAGAGTC CCCCCACCAA 
CCAGAACCTA AGATAACTTA CCCGTTTCAC GGATACAATA TGGGAAATAT ACCCACTCAA 
ATCTCAGGGT CAAATTACTA CCTGTATCCT TCTGATGGGG GGCAGATTGA AGTGGAAAAG 
ACAATGTTTG AAAAAGGCCC AACTTTTATC AAAGTGAGAG TGGTAAAGGA CAACCACAAT 
TTTATGATTC ATTTAAATGA CCCTAAGGAA ATTGTGAAAA AGGAAGAGAA AGGGGATTAT 
TATTTAGAAG AGGAAGAAGA AGGAGATGAG GAACAAAGTC TTGAAACGGA AGTATCAGAT 
GCAAAGAATA AAGCTTTCAG CAAGTTTGGA TCTTTTTCTG CCACCTTAGA AAATGGAATC 
TGCCTCTCGA TAAGTTACTA TGGATCAAAT GGAATGGCAC CAGAAGATAA GGATCCTGAT 
TTAGAAACAA TATTGAATAT CCCTTCAGCA CTCACTCCAA CAGTGGTTCC TGTTATAGTG 
ACCGTTCCTC AAAGCAAAGC TAAAGGGAAA ATAAAAGGCA AAGAAAAACC CAAAGAATCC 
CTTAAAGAAG AAGAACACCC AAAAGAAGAA GAGAAAAAGG AAGAAGAAGT AGAACCAGAA 
CCTGTTTTAC AAGAGACTTT GGATGTTCCC ACCTTCCAGA GCCTAAATGT GTCTTGCCCC 
AGTGGGCTCC TGTTGACTTT CATTGGACAA GAATCTACAG GTCAATATGT TATAGATGAG 
GAACCCACCT GGGACATCAT GGTCCGTCAG AGCTACCCCC AGAGGGTGAA GCACTATGAG 
TTCTATAAAA CGGTGATGCC ACCCGCAGAG CAGGAGGCTT CAAGGGTTAT CACCAGTCAA 
GGCACTGTTG TCAAATATAT GTTGGATGGA TCCACACAGA TTCTCTTTGC AGATGGTGCT 
GTGAGCAGGA GTCCCAATTC AGGTCTTATT TGTCCTCCTT CTGAAATGCC AGCAACGCCT 
CACAGTGGAG ATTTGATGGA CTCTATTTCT CAGCAGAAAT CAGAAACGAT ACCATCTGAG 
ATTACCAACA CAAAGAAAGG AAAAAGTCAC AAAAGTCAGT CATCAATGGC CCATAAGGGT 
GAAATCCATG ACCCTCCTCC AGAGGCAGTT CAAACTGTAA CTCCTGTGGA GGTTCACATA 
GGCACCTGGT TTACAACCAC ACCTGAAGGA AATCGGATCG GCACCAAAGG ATTAGAAAGA 
ATAGCAGACT TGACCCCATT GTTATCCTTT CAGGCCACAG ATCCTGTCAA TGGAACGGTT 
ATGACAACTC GAGAAGACAA AGTTGTCATA GTTGAAAGGA AAGATGGTAC TCGGATAGTG 
GATCATGCTG ATGGTACCAG AATCACAACC TTTTATCAAG TTTATGAAGA TCAAATTATT 
CTGCCAGATG ATCAAGAAAC AACCGAGGGT CCTCGGACTG TCACCAGGCA GGTGAAGTGT 
ATGCGGGTAG AAAGCTCACG CTATGCCACT GTTATCGCCA ACTGTGAGGA CAGTAGCTGC 
TGTGCCACCT TTGGAGATGG AACAACTATT ATTGCAAAGC CACAGGGAAC ATACCAGGTG 
TTACCTCCAA ACACAGGCTC TCTTTATATT GACAAGGATT GTTCAGCTGT GTACTGCCAT 
GAGTCAAGCA GTAATATATA CTATCCTTTT CAAAAGCGTG AGCAGCTGCG AGCTGGCAGG 
TACATCATGA GGCATACTTC AGAGGTTATC TGTGAGGTTC TGGATCCTGA GGGAAACACT 
TTTCAGGTCA TGGCTGATGG TAGCATATCA ACTATATTAC CTGAAAAAAA ATTGGAAGAT 
GATTTAAATG AGAAAACTGA GGGCTATGAT AGTCTGTCCT CTATGCACCT TGAAAAGAAT 
CATCAGCAAA TCTATGGTGA ACATGTCCCC AGGTTTTTTG TTATGTATGC TGATGGATCA 
GGAATGGAAC TTCTTCGAGA CAGTGACATA GAAGAATATC TATCTTTGGC ATATAAAGAA 
TCAAATACTG TTGTTCTCCA AGAGCCAGTG CAGGAACAGC CAGGCACCCT AACCATCACA 
GTCCTTCGCC CTTTCCATGA AGCATCACCA TGGCAAGTAA AAAAGGAAGA TACAATTGTC 
CCTCCTAATC TCCGGTCAAG GTCATGGGAA ACATTTCCCT CAGTTGAGAA AAAAACTCCA 
GGACCTCCGT TTGGTACTCA GATTTGGAAA GGCCTTTGCA TTGAGTCCAA ACAGCTAGTG 
AGTGCCCCGG GTGCCATACT CAAGAGCCCC AGTGTGCTAC AGATGCGCCA ATTCATTCAG 
CATGAGGTCA TAAAGAATGA GGTGAAACTG AGGCTGCAGG TTTCCCTTAA GGATTACATA 
AACTATATTC TAAAGAAAGA AGATGAGCTG CAGGAAATGA TGGTTAAAGA TTCCAGAACT 
GAGGAGGAGA GAGGCAATGC TGCTGATCTC CTCAAGCTGG TTATGTCTTT CCCTAAAATG 
GAGGAAACTA CAAAAAGTCA TGTTACTGAA GTTGCAGCTC ACCTAACTGA TTTATTCAAG 
CAGTCTTTGG CTACGCCTCC AAAATGCCCA CCAGACACAT TTGGTAAAGA TTTCTTTGAA 
AAGACATGGA GACACACAGC ATCCTCAAAA CGCTGGAAAG AAAAGATAGA CAAAACGAGG 
AAGGAAATTG AGACAACACA GAATTACCTA ATGGATATTA AGAACCGCAT AATACCACCC 
TTTTTTAAAT CTGAATTGAA CCAGTTATAT CAGTCTCAGT ATAATCACCT GGACAGTCTT 
TCCAAAAAAC TGCCTTCTTT TACAAAGAAA AATGAAGATG CAAACGAAAC AGCTGTTCAA 
GATACATCTG ATCTTAATCT AGATTTCAAG CCACATAAGG TTTCAGAACA GAAATCCTCA 
AGTGTGCCTA GTCTTCCAAA ACCAGAGATT TCTGCAGATA AGAAGGATTT CACTGCTCAG 
AACCAAACTG AAAATTTAAC AAAATCTCCT GAAGAAGCAG AATCTTATGA GCCCGTGAAA 
ATTCCAACCC AGTCCTTGCT GCAGGATGTT GCGGGACAAA CAAGAAAAGA AAAAGTGAAG 
TTGCCTCATT ATTTGCTGAG TTCCAAGCCT AAGTCTCAAC CTCTTGCAAA GGTGCAAGAT 
TCTGTTGGAG GAAAAGTGAA CACATCCTCT GTTGCATCTG CTGCCATTAA TAATGCAAAG 
TCATCCCTTT TTGGGTTCCA TCTTCTCCCA TCATCAGTCA AGTTTGGAGT GCTTAAGGAA 
GGACATACCT ATGCCACAGT TGTAAAGCTC AAGAATGTTG GAGTGGACTT CTGCAGGTTT 
AAAGTAAAGC AGCCCCCACC CAGCACAGGA CTGAAAGTGA CTTACAAACC TGGACCTGTG 
GCAGCTGGTA TGCAGACAGA ACTGAATATA GAGTTATTTG CCACAGCTGT TGGAGAGGAT 
GGGGCCAAGG GATCAGCACA CATCTCTCAC AATATCGAGA TTATGACAGA GCATGAGGTT 
CTGTTCCTAC CTGTGGAAGC AACTGTTTTA ACAAGCAGCA ATTATGATAA ACGACCAAAA 
GACTTTCCCC AGGGAAAAGA AAATCCAATG GTCCAGAGAA CTTCTACAAT TTATTCCTCC 
ACACTTGGAG TCTTCATGTC TCGTAAAGTT TCTCCACATT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011539239.1
CDS	543..5564
Translation	MQSKLPLWEFLQFPLPPPWNNTKRLATIHELMHFCTSDVLSWNEVERAFKVFTFESLKLSEVDEKGKLKPSGMMCGSDSEMFNIPWDNPARFAKQIRQQYVMKMNTQEAKQKADIKIKDRTLFVDQNLSMSVQDNESNREPSDPSQCDANNMKHSDLNNLKLSVPDNRQLLEQESIMKAQPQHESLEQTTNNEIKDDAVTKADSHEKKPKKMMVEADLEDIKKTQQRSLMDWSFTEHFKPKVLLQVLQEAHKQYRCVDSYYHTQDNSLLLVFHNPMNRQRLHCEYWNIALHSNVGFRNYLELVAKSIQDWITKEEAIYQESKMNEKIIRTRAELELKSSANAKLTSASKIFSIKESKSNKGISKTEISDQEKEKEKEKIPFILEGSLKAWKEEQHRLAEEERLREEKKAEKKGKEAGKKKGKDNAEKEDSRSLKKKSPYKEKSKEEQVKIQEVTEESPHQPEPKITYPFHGYNMGNIPTQISGSNYYLYPSDGGQIEVEKTMFEKGPTFIKVRVVKDNHNFMIHLNDPKEIVKKEEKGDYYLEEEEEGDEEQSLETEVSDAKNKAFSKFGSFSATLENGICLSISYYGSNGMAPEDKDPDLETILNIPSALTPTVVPVIVTVPQSKAKGKIKGKEKPKESLKEEEHPKEEEKKEEEVEPEPVLQETLDVPTFQSLNVSCPSGLLLTFIGQESTGQYVIDEEPTWDIMVRQSYPQRVKHYEFYKTVMPPAEQEASRVITSQGTVVKYMLDGSTQILFADGAVSRSPNSGLICPPSEMPATPHSGDLMDSISQQKSETIPSEITNTKKGKSHKSQSSMAHKGEIHDPPPEAVQTVTPVEVHIGTWFTTTPEGNRIGTKGLERIADLTPLLSFQATDPVNGTVMTTREDKVVIVERKDGTRIVDHADGTRITTFYQVYEDQIILPDDQETTEGPRTVTRQVKCMRVESSRYATVIANCEDSSCCATFGDGTTIIAKPQGTYQVLPPNTGSLYIDKDCSAVYCHESSSNIYYPFQKREQLRAGRYIMRHTSEVICEVLDPEGNTFQVMADGSISTILPEKKLEDDLNEKTEGYDSLSSMHLEKNHQQIYGEHVPRFFVMYADGSGMELLRDSDIEEYLSLAYKESNTVVLQEPVQEQPGTLTITVLRPFHEASPWQVKKEDTIVPPNLRSRSWETFPSVEKKTPGPPFGTQIWKGLCIESKQLVSAPGAILKSPSVLQMRQFIQHEVIKNEVKLRLQVSLKDYINYILKKEDELQEMMVKDSRTEEERGNAADLLKLVMSFPKMEETTKSHVTEVAAHLTDLFKQSLATPPKCPPDTFGKDFFEKTWRHTASSKRWKEKIDKTRKEIETTQNYLMDIKNRIIPPFFKSELNQLYQSQYNHLDSLSKKLPSFTKKNEDANETAVQDTSDLNLDFKPHKVSEQKSSSVPSLPKPEISADKKDFTAQNQTENLTKSPEEAESYEPVKIPTQSLLQDVAGQTRKEKVKLPHYLLSSKPKSQPLAKVQDSVGGKVNTSSVASAAINNAKSSLFGFHLLPSSVKFGVLKEGHTYATVVKLKNVGVDFCRFKVKQPPPSTGLKVTYKPGPVAAGMQTELNIELFATAVGEDGAKGSAHISHNIEIMTEHEVLFLPVEATVLTSSNYDKRPKDFPQGKENPMVQRTSTIYSSTLGVFMSRKVSPH

Target ORF information:

RefSeq Version	XM_011540937.2
Organism	Homo sapiens(human)
Definition	Homo sapiens sperm associated antigen 17 (SPAG17), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011540937.2

ORF Insert Sequence:

ATGCAGTCCA AGTTGCCACT GTGGGAATTT CTTCAATTCC CTCTACCCCC ACCATGGAAC 
AACACTAAAC GTCTAGCTAC AATTCATGAG CTTATGCACT TTTGTACGAG TGATGTCTTG 
AGCTGGAATG AAGTAGAACG AGCCTTCAAG GTGTTTACTT TTGAGAGCCT GAAGCTCTCT 
GAGGTTGATG AAAAAGGGAA ACTGAAACCT TCTGGGATGA TGTGTGGGTC AGATTCTGAA 
ATGTTCAACA TACCGTGGGA CAACCCTGCC AGATTTGCTA AACAGATAAG GCAGCAATAT 
GTCATGAAAA TGAATACTCA AGAGGCCAAG CAGAAAGCAG ATATTAAAAT CAAAGACAGA 
ACACTATTTG TGGATCAGAA TTTGTCAATG TCTGTGCAAG ATAATGAAAG CAACCGAGAA 
CCTTCAGATC CTAGTCAGTG TGATGCTAAC AATATGAAGC ATTCTGACTT GAATAATCTC 
AAACTCTCAG TCCCTGATAA TAGACAGCTG TTAGAGCAGG AGAGCATCAT GAAGGCTCAG 
CCCCAACATG AGTCTCTGGA GCAGACCACA AACAATGAGA TCAAAGATGA TGCAGTCACA 
AAGGCTGATT CTCATGAAAA GAAACCCAAG AAGATGATGG TGGAAGCAGA TTTAGAGGAC 
ATAAAGAAAA CACAGCAGCG CAGTCTAATG GACTGGAGTT TTACTGAACA TTTTAAACCG 
AAAGTACTGC TTCAGGTCCT TCAAGAAGCC CATAAGCAAT ATAGGTGTGT TGATTCTTAC 
TACCACACCC AAGACAACTC TTTACTTTTA GTCTTTCACA ATCCAATGAA TAGACAACGT 
TTGCATTGTG AATATTGGAA CATTGCTCTC CACTCCAATG TTGGATTCAG GAATTATTTG 
GAACTTGTTG CAAAATCTAT TCAAGATTGG ATTACAAAAG AAGAAGCTAT ATATCAGGAA 
TCTAAAATGA ATGAGAAAAT CATCAGGACC AGAGCTGAGC TGGAATTGAA ATCTTCTGCT 
AATGCCAAAC TTACTTCTGC TAGCAAAATT TTTTCCATTA AAGAATCTAA AAGTAACAAA 
GGAATCAGCA AAACAGAGAT ATCAGATCAA GAAAAAGAAA AAGAGAAGGA AAAGATTCCT 
TTCATTTTAG AAGGCTCTCT CAAGGCATGG AAAGAAGAGC AACATCGATT AGCAGAAGAG 
GAGCGCTTAA GGGAAGAAAA GAAAGCAGAG AAGAAGGGTA AAGAAGCTGG TAAAAAGAAA 
GGCAAGGATA ACGCAGAGAA AGAGGATAGT AGGTCTTTGA AGAAAAAATC ACCTTACAAG 
GAGAAATCTA AAGAAGAACA AGTCAAGATC CAAGAAGTAA CAGAAGAGTC CCCCCACCAA 
CCAGAACCTA AGATAACTTA CCCGTTTCAC GGATACAATA TGGGAAATAT ACCCACTCAA 
ATCTCAGGGT CAAATTACTA CCTGTATCCT TCTGATGGGG GGCAGATTGA AGTGGAAAAG 
ACAATGTTTG AAAAAGGCCC AACTTTTATC AAAGTGAGAG TGGTAAAGGA CAACCACAAT 
TTTATGATTC ATTTAAATGA CCCTAAGGAA ATTGTGAAAA AGGAAGAGAA AGGGGATTAT 
TATTTAGAAG AGGAAGAAGA AGGAGATGAG GAACAAAGTC TTGAAACGGA AGTATCAGAT 
GCAAAGAATA AAGCTTTCAG CAAGTTTGGA TCTTTTTCTG CCACCTTAGA AAATGGAATC 
TGCCTCTCGA TAAGTTACTA TGGATCAAAT GGAATGGCAC CAGAAGATAA GGATCCTGAT 
TTAGAAACAA TATTGAATAT CCCTTCAGCA CTCACTCCAA CAGTGGTTCC TGTTATAGTG 
ACCGTTCCTC AAAGCAAAGC TAAAGGGAAA ATAAAAGGCA AAGAAAAACC CAAAGAATCC 
CTTAAAGAAG AAGAACACCC AAAAGAAGAA GAGAAAAAGG AAGAAGAAGT AGAACCAGAA 
CCTGTTTTAC AAGAGACTTT GGATGTTCCC ACCTTCCAGA GCCTAAATGT GTCTTGCCCC 
AGTGGGCTCC TGTTGACTTT CATTGGACAA GAATCTACAG GTCAATATGT TATAGATGAG 
GAACCCACCT GGGACATCAT GGTCCGTCAG AGCTACCCCC AGAGGGTGAA GCACTATGAG 
TTCTATAAAA CGGTGATGCC ACCCGCAGAG CAGGAGGCTT CAAGGGTTAT CACCAGTCAA 
GGCACTGTTG TCAAATATAT GTTGGATGGA TCCACACAGA TTCTCTTTGC AGATGGTGCT 
GTGAGCAGGA GTCCCAATTC AGGTCTTATT TGTCCTCCTT CTGAAATGCC AGCAACGCCT 
CACAGTGGAG ATTTGATGGA CTCTATTTCT CAGCAGAAAT CAGAAACGAT ACCATCTGAG 
ATTACCAACA CAAAGAAAGG AAAAAGTCAC AAAAGTCAGT CATCAATGGC CCATAAGGGT 
GAAATCCATG ACCCTCCTCC AGAGGCAGTT CAAACTGTAA CTCCTGTGGA GGTTCACATA 
GGCACCTGGT TTACAACCAC ACCTGAAGGA AATCGGATCG GCACCAAAGG ATTAGAAAGA 
ATAGCAGACT TGACCCCATT GTTATCCTTT CAGGCCACAG ATCCTGTCAA TGGAACGGTT 
ATGACAACTC GAGAAGACAA AGTTGTCATA GTTGAAAGGA AAGATGGTAC TCGGATAGTG 
GATCATGCTG ATGGTACCAG AATCACAACC TTTTATCAAG TTTATGAAGA TCAAATTATT 
CTGCCAGATG ATCAAGAAAC AACCGAGGGT CCTCGGACTG TCACCAGGCA GGTGAAGTGT 
ATGCGGGTAG AAAGCTCACG CTATGCCACT GTTATCGCCA ACTGTGAGGA CAGTAGCTGC 
TGTGCCACCT TTGGAGATGG AACAACTATT ATTGCAAAGC CACAGGGAAC ATACCAGGTG 
TTACCTCCAA ACACAGGCTC TCTTTATATT GACAAGGATT GTTCAGCTGT GTACTGCCAT 
GAGTCAAGCA GTAATATATA CTATCCTTTT CAAAAGCGTG AGCAGCTGCG AGCTGGCAGG 
TACATCATGA GGCATACTTC AGAGGTTATC TGTGAGGTTC TGGATCCTGA GGGAAACACT 
TTTCAGGTCA TGGCTGATGG TAGCATATCA ACTATATTAC CTGAAAAAAA ATTGGAAGAT 
GATTTAAATG AGAAAACTGA GGGCTATGAT AGTCTGTCCT CTATGCACCT TGAAAAGAAT 
CATCAGCAAA TCTATGGTGA ACATGTCCCC AGGTTTTTTG TTATGTATGC TGATGGATCA 
GGAATGGAAC TTCTTCGAGA CAGTGACATA GAAGAATATC TATCTTTGGC ATATAAAGAA 
TCAAATACTG TTGTTCTCCA AGAGCCAGTG CAGGAACAGC CAGGCACCCT AACCATCACA 
GTCCTTCGCC CTTTCCATGA AGCATCACCA TGGCAAGTAA AAAAGGAAGA TACAATTGTC 
CCTCCTAATC TCCGGTCAAG GTCATGGGAA ACATTTCCCT CAGTTGAGAA AAAAACTCCA 
GGACCTCCGT TTGGTACTCA GATTTGGAAA GGCCTTTGCA TTGAGTCCAA ACAGCTAGTG 
AGTGCCCCGG GTGCCATACT CAAGAGCCCC AGTGTGCTAC AGATGCGCCA ATTCATTCAG 
CATGAGGTCA TAAAGAATGA GGTGAAACTG AGGCTGCAGG TTTCCCTTAA GGATTACATA 
AACTATATTC TAAAGAAAGA AGATGAGCTG CAGGAAATGA TGGTTAAAGA TTCCAGAACT 
GAGGAGGAGA GAGGCAATGC TGCTGATCTC CTCAAGCTGG TTATGTCTTT CCCTAAAATG 
GAGGAAACTA CAAAAAGTCA TGTTACTGAA GTTGCAGCTC ACCTAACTGA TTTATTCAAG 
CAGTCTTTGG CTACGCCTCC AAAATGCCCA CCAGACACAT TTGGTAAAGA TTTCTTTGAA 
AAGACATGGA GACACACAGC ATCCTCAAAA CGCTGGAAAG AAAAGATAGA CAAAACGAGG 
AAGGAAATTG AGACAACACA GAATTACCTA ATGGATATTA AGAACCGCAT AATACCACCC 
TTTTTTAAAT CTGAATTGAA CCAGTTATAT CAGTCTCAGT ATAATCACCT GGACAGTCTT 
TCCAAAAAAC TGCCTTCTTT TACAAAGAAA AATGAAGATG CAAACGAAAC AGCTGTTCAA 
GATACATCTG ATCTTAATCT AGATTTCAAG CCACATAAGG TTTCAGAACA GAAATCCTCA 
AGTGTGCCTA GTCTTCCAAA ACCAGAGATT TCTGCAGATA AGAAGGATTT CACTGCTCAG 
AACCAAACTG AAAATTTAAC AAAATCTCCT GAAGAAGCAG AATCTTATGA GCCCGTGAAA 
ATTCCAACCC AGTCCTTGCT GCAGGATGTT GCGGGACAAA CAAGAAAAGA AAAAGTGAAG 
TTGCCTCATT ATTTGCTGAG TTCCAAGCCT AAGTCTCAAC CTCTTGCAAA GGTGCAAGAT 
TCTGTTGGAG GAAAAGTGAA CACATCCTCT GTTGCATCTG CTGCCATTAA TAATGCAAAG 
TCATCCCTTT TTGGGTTCCA TCTTCTCCCA TCATCAGTCA AGTTTGGAGT GCTTAAGGAA 
GGACATACCT ATGCCACAGT TGTAAAGCTC AAGAATGTTG GAGTGGACTT CTGCAGGTTT 
AAAGTAAAGC AGCCCCCACC CAGCACAGGA CTGAAAGTGA CTTACAAACC TGGACCTGTG 
GCAGCTGGTA TGCAGACAGA ACTGAATATA GAGTTATTTG CCACAGCTGT TGGAGAGGAT 
GGGGCCAAGG GATCAGCACA CATCTCTCAC AATATCGAGA TTATGACAGA GCATGAGGTT 
CTGTTCCTAC CTGTGGAAGC AACTGTTTTA ACAAGCAGCA ATTATGATAA ACGACCAAAA 
GACTTTCCCC AGGGAAAAGA AAATCCAATG GTCCAGAGAA CTTCTACAAT TTATTCCTCC 
ACACTTGGAG TCTTCATGTC TCGTAAAGTT TCTCCACATT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPAG17 ( XM_011540941.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011539243.1 Homo sapiens sperm associated antigen 17 (SPAG17), transcript variant X10, mRNA.

Price & Availability↑

CloneID	OHu77639
Clone ID Related Accession (Same CDS sequence)	XM_011540941.2 , XM_011540942.2
Accession Version	XM_011540941.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4389bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	sperm-associated antigen 17 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011540941.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGTGG AAGCAGATTT AGAGGACATA AAGAAAACAC AGCAGCGCAG TCTAATGGAC 
TGGAGTTTTA CTGAACATTT TAAACCGAAA GTACTGCTTC AGGTCCTTCA AGAAGCCCAT 
AAGCAATATA GGTGTGTTGA TTCTTACTAC CACACCCAAG ACAACTCTTT ACTTTTAGTC 
TTTCACAATC CAATGAATAG ACAACGTTTG CATTGTGAAT ATTGGAACAT TGCTCTCCAC 
TCCAATGTTG GATTCAGGAA TTATTTGGAA CTTGTTGCAA AATCTATTCA AGATTGGATT 
ACAAAAGAAG AAGCTATATA TCAGGAATCT AAAATGAATG AGAAAATCAT CAGGACCAGA 
GCTGAGCTGG AATTGAAATC TTCTGCTAAT GCCAAACTTA CTTCTGCTAG CAAAATTTTT 
TCCATTAAAG AATCTAAAAG TAACAAAGGA ATCAGCAAAA CAGAGATATC AGATCAAGAA 
AAAGAAAAAG AGAAGGAAAA GATTCCTTTC ATTTTAGAAG GCTCTCTCAA GGCATGGAAA 
GAAGAGCAAC ATCGATTAGC AGAAGAGGAG CGCTTAAGGG AAGAAAAGAA AGCAGAGAAG 
AAGGGTAAAG AAGCTGGTAA AAAGAAAGGC AAGGATAACG CAGAGAAAGA GGATAGTAGG 
TCTTTGAAGA AAAAATCACC TTACAAGGAG AAATCTAAAG AAGAACAAGT CAAGATCCAA 
GAAGTAACAG AAGAGTCCCC CCACCAACCA GAACCTAAGA TAACTTACCC GTTTCACGGA 
TACAATATGG GAAATATACC CACTCAAATC TCAGGGTCAA ATTACTACCT GTATCCTTCT 
GATGGGGGGC AGATTGAAGT GGAAAAGACA ATGTTTGAAA AAGGCCCAAC TTTTATCAAA 
GTGAGAGTGG TAAAGGACAA CCACAATTTT ATGATTCATT TAAATGACCC TAAGGAAATT 
GTGAAAAAGG AAGAGAAAGG GGATTATTAT TTAGAAGAGG AAGAAGAAGG AGATGAGGAA 
CAAAGTCTTG AAACGGAAGT ATCAGATGCA AAGAATAAAG CTTTCAGCAA GTTTGGATCT 
TTTTCTGCCA CCTTAGAAAA TGGAATCTGC CTCTCGATAA GTTACTATGG ATCAAATGGA 
ATGGCACCAG AAGATAAGGA TCCTGATTTA GAAACAATAT TGAATATCCC TTCAGCACTC 
ACTCCAACAG TGGTTCCTGT TATAGTGACC GTTCCTCAAA GCAAAGCTAA AGGGAAAATA 
AAAGGCAAAG AAAAACCCAA AGAATCCCTT AAAGAAGAAG AACACCCAAA AGAAGAAGAG 
AAAAAGGAAG AAGAAGTAGA ACCAGAACCT GTTTTACAAG AGACTTTGGA TGTTCCCACC 
TTCCAGAGCC TAAATGTGTC TTGCCCCAGT GGGCTCCTGT TGACTTTCAT TGGACAAGAA 
TCTACAGGTC AATATGTTAT AGATGAGGAA CCCACCTGGG ACATCATGGT CCGTCAGAGC 
TACCCCCAGA GGGTGAAGCA CTATGAGTTC TATAAAACGG TGATGCCACC CGCAGAGCAG 
GAGGCTTCAA GGGTTATCAC CAGTCAAGGC ACTGTTGTCA AATATATGTT GGATGGATCC 
ACACAGATTC TCTTTGCAGA TGGTGCTGTG AGCAGGAGTC CCAATTCAGG TCTTATTTGT 
CCTCCTTCTG AAATGCCAGC AACGCCTCAC AGTGGAGATT TGATGGACTC TATTTCTCAG 
CAGAAATCAG AAACGATACC ATCTGAGATT ACCAACACAA AGAAAGGAAA AAGTCACAAA 
AGTCAGTCAT CAATGGCCCA TAAGGGTGAA ATCCATGACC CTCCTCCAGA GGCAGTTCAA 
ACTGTAACTC CTGTGGAGGT TCACATAGGC ACCTGGTTTA CAACCACACC TGAAGGAAAT 
CGGATCGGCA CCAAAGGATT AGAAAGAATA GCAGACTTGA CCCCATTGTT ATCCTTTCAG 
GCCACAGATC CTGTCAATGG AACGGTTATG ACAACTCGAG AAGACAAAGT TGTCATAGTT 
GAAAGGAAAG ATGGTACTCG GATAGTGGAT CATGCTGATG GTACCAGAAT CACAACCTTT 
TATCAAGTTT ATGAAGATCA AATTATTCTG CCAGATGATC AAGAAACAAC CGAGGGTCCT 
CGGACTGTCA CCAGGCAGGT GAAGTGTATG CGGGTAGAAA GCTCACGCTA TGCCACTGTT 
ATCGCCAACT GTGAGGACAG TAGCTGCTGT GCCACCTTTG GAGATGGAAC AACTATTATT 
GCAAAGCCAC AGGGAACATA CCAGGTGTTA CCTCCAAACA CAGGCTCTCT TTATATTGAC 
AAGGATTGTT CAGCTGTGTA CTGCCATGAG TCAAGCAGTA ATATATACTA TCCTTTTCAA 
AAGCGTGAGC AGCTGCGAGC TGGCAGGTAC ATCATGAGGC ATACTTCAGA GGTTATCTGT 
GAGGTTCTGG ATCCTGAGGG AAACACTTTT CAGGTCATGG CTGATGGTAG CATATCAACT 
ATATTACCTG AAAAAAAATT GGAAGATGAT TTAAATGAGA AAACTGAGGG CTATGATAGT 
CTGTCCTCTA TGCACCTTGA AAAGAATCAT CAGCAAATCT ATGGTGAACA TGTCCCCAGG 
TTTTTTGTTA TGTATGCTGA TGGATCAGGA ATGGAACTTC TTCGAGACAG TGACATAGAA 
GAATATCTAT CTTTGGCATA TAAAGAATCA AATACTGTTG TTCTCCAAGA GCCAGTGCAG 
GAACAGCCAG GCACCCTAAC CATCACAGTC CTTCGCCCTT TCCATGAAGC ATCACCATGG 
CAAGTAAAAA AGGAAGATAC AATTGTCCCT CCTAATCTCC GGTCAAGGTC ATGGGAAACA 
TTTCCCTCAG TTGAGAAAAA AACTCCAGGA CCTCCGTTTG GTACTCAGAT TTGGAAAGGC 
CTTTGCATTG AGTCCAAACA GCTAGTGAGT GCCCCGGGTG CCATACTCAA GAGCCCCAGT 
GTGCTACAGA TGCGCCAATT CATTCAGCAT GAGGTCATAA AGAATGAGGT GAAACTGAGG 
CTGCAGGTTT CCCTTAAGGA TTACATAAAC TATATTCTAA AGAAAGAAGA TGAGCTGCAG 
GAAATGATGG TTAAAGATTC CAGAACTGAG GAGGAGAGAG GCAATGCTGC TGATCTCCTC 
AAGCTGGTTA TGTCTTTCCC TAAAATGGAG GAAACTACAA AAAGTCATGT TACTGAAGTT 
GCAGCTCACC TAACTGATTT ATTCAAGCAG TCTTTGGCTA CGCCTCCAAA ATGCCCACCA 
GACACATTTG GTAAAGATTT CTTTGAAAAG ACATGGAGAC ACACAGCATC CTCAAAACGC 
TGGAAAGAAA AGATAGACAA AACGAGGAAG GAAATTGAGA CAACACAGAA TTACCTAATG 
GATATTAAGA ACCGCATAAT ACCACCCTTT TTTAAATCTG AATTGAACCA GTTATATCAG 
TCTCAGTATA ATCACCTGGA CAGTCTTTCC AAAAAACTGC CTTCTTTTAC AAAGAAAAAT 
GAAGATGCAA ACGAAACAGC TGTTCAAGAT ACATCTGATC TTAATCTAGA TTTCAAGCCA 
CATAAGGTTT CAGAACAGAA ATCCTCAAGT GTGCCTAGTC TTCCAAAACC AGAGATTTCT 
GCAGATAAGA AGGATTTCAC TGCTCAGAAC CAAACTGAAA ATTTAACAAA ATCTCCTGAA 
GAAGCAGAAT CTTATGAGCC CGTGAAAATT CCAACCCAGT CCTTGCTGCA GGATGTTGCG 
GGACAAACAA GAAAAGAAAA AGTGAAGTTG CCTCATTATT TGCTGAGTTC CAAGCCTAAG 
TCTCAACCTC TTGCAAAGGT GCAAGATTCT GTTGGAGGAA AAGTGAACAC ATCCTCTGTT 
GCATCTGCTG CCATTAATAA TGCAAAGTCA TCCCTTTTTG GGTTCCATCT TCTCCCATCA 
TCAGTCAAGT TTGGAGTGCT TAAGGAAGGA CATACCTATG CCACAGTTGT AAAGCTCAAG 
AATGTTGGAG TGGACTTCTG CAGGTTTAAA GTAAAGCAGC CCCCACCCAG CACAGGACTG 
AAAGTGACTT ACAAACCTGG ACCTGTGGCA GCTGGTATGC AGACAGAACT GAATATAGAG 
TTATTTGCCA CAGCTGTTGG AGAGGATGGG GCCAAGGGAT CAGCACACAT CTCTCACAAT 
ATCGAGATTA TGACAGAGCA TGAGGTTCTG TTCCTACCTG TGGAAGCAAC TGTTTTAACA 
AGCAGCAATT ATGATAAACG ACCAAAAGAC TTTCCCCAGG GAAAAGAAAA TCCAATGGTC 
CAGAGAACTT CTACAATTTA TTCCTCCACA CTTGGAGTCT TCATGTCTCG TAAAGTTTCT 
CCACATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011539243.1
CDS	471..4859
Translation	MMVEADLEDIKKTQQRSLMDWSFTEHFKPKVLLQVLQEAHKQYRCVDSYYHTQDNSLLLVFHNPMNRQRLHCEYWNIALHSNVGFRNYLELVAKSIQDWITKEEAIYQESKMNEKIIRTRAELELKSSANAKLTSASKIFSIKESKSNKGISKTEISDQEKEKEKEKIPFILEGSLKAWKEEQHRLAEEERLREEKKAEKKGKEAGKKKGKDNAEKEDSRSLKKKSPYKEKSKEEQVKIQEVTEESPHQPEPKITYPFHGYNMGNIPTQISGSNYYLYPSDGGQIEVEKTMFEKGPTFIKVRVVKDNHNFMIHLNDPKEIVKKEEKGDYYLEEEEEGDEEQSLETEVSDAKNKAFSKFGSFSATLENGICLSISYYGSNGMAPEDKDPDLETILNIPSALTPTVVPVIVTVPQSKAKGKIKGKEKPKESLKEEEHPKEEEKKEEEVEPEPVLQETLDVPTFQSLNVSCPSGLLLTFIGQESTGQYVIDEEPTWDIMVRQSYPQRVKHYEFYKTVMPPAEQEASRVITSQGTVVKYMLDGSTQILFADGAVSRSPNSGLICPPSEMPATPHSGDLMDSISQQKSETIPSEITNTKKGKSHKSQSSMAHKGEIHDPPPEAVQTVTPVEVHIGTWFTTTPEGNRIGTKGLERIADLTPLLSFQATDPVNGTVMTTREDKVVIVERKDGTRIVDHADGTRITTFYQVYEDQIILPDDQETTEGPRTVTRQVKCMRVESSRYATVIANCEDSSCCATFGDGTTIIAKPQGTYQVLPPNTGSLYIDKDCSAVYCHESSSNIYYPFQKREQLRAGRYIMRHTSEVICEVLDPEGNTFQVMADGSISTILPEKKLEDDLNEKTEGYDSLSSMHLEKNHQQIYGEHVPRFFVMYADGSGMELLRDSDIEEYLSLAYKESNTVVLQEPVQEQPGTLTITVLRPFHEASPWQVKKEDTIVPPNLRSRSWETFPSVEKKTPGPPFGTQIWKGLCIESKQLVSAPGAILKSPSVLQMRQFIQHEVIKNEVKLRLQVSLKDYINYILKKEDELQEMMVKDSRTEEERGNAADLLKLVMSFPKMEETTKSHVTEVAAHLTDLFKQSLATPPKCPPDTFGKDFFEKTWRHTASSKRWKEKIDKTRKEIETTQNYLMDIKNRIIPPFFKSELNQLYQSQYNHLDSLSKKLPSFTKKNEDANETAVQDTSDLNLDFKPHKVSEQKSSSVPSLPKPEISADKKDFTAQNQTENLTKSPEEAESYEPVKIPTQSLLQDVAGQTRKEKVKLPHYLLSSKPKSQPLAKVQDSVGGKVNTSSVASAAINNAKSSLFGFHLLPSSVKFGVLKEGHTYATVVKLKNVGVDFCRFKVKQPPPSTGLKVTYKPGPVAAGMQTELNIELFATAVGEDGAKGSAHISHNIEIMTEHEVLFLPVEATVLTSSNYDKRPKDFPQGKENPMVQRTSTIYSSTLGVFMSRKVSPH

Target ORF information:

RefSeq Version	XM_011540941.2
Organism	Homo sapiens(human)
Definition	Homo sapiens sperm associated antigen 17 (SPAG17), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011540941.2

ORF Insert Sequence:

ATGATGGTGG AAGCAGATTT AGAGGACATA AAGAAAACAC AGCAGCGCAG TCTAATGGAC 
TGGAGTTTTA CTGAACATTT TAAACCGAAA GTACTGCTTC AGGTCCTTCA AGAAGCCCAT 
AAGCAATATA GGTGTGTTGA TTCTTACTAC CACACCCAAG ACAACTCTTT ACTTTTAGTC 
TTTCACAATC CAATGAATAG ACAACGTTTG CATTGTGAAT ATTGGAACAT TGCTCTCCAC 
TCCAATGTTG GATTCAGGAA TTATTTGGAA CTTGTTGCAA AATCTATTCA AGATTGGATT 
ACAAAAGAAG AAGCTATATA TCAGGAATCT AAAATGAATG AGAAAATCAT CAGGACCAGA 
GCTGAGCTGG AATTGAAATC TTCTGCTAAT GCCAAACTTA CTTCTGCTAG CAAAATTTTT 
TCCATTAAAG AATCTAAAAG TAACAAAGGA ATCAGCAAAA CAGAGATATC AGATCAAGAA 
AAAGAAAAAG AGAAGGAAAA GATTCCTTTC ATTTTAGAAG GCTCTCTCAA GGCATGGAAA 
GAAGAGCAAC ATCGATTAGC AGAAGAGGAG CGCTTAAGGG AAGAAAAGAA AGCAGAGAAG 
AAGGGTAAAG AAGCTGGTAA AAAGAAAGGC AAGGATAACG CAGAGAAAGA GGATAGTAGG 
TCTTTGAAGA AAAAATCACC TTACAAGGAG AAATCTAAAG AAGAACAAGT CAAGATCCAA 
GAAGTAACAG AAGAGTCCCC CCACCAACCA GAACCTAAGA TAACTTACCC GTTTCACGGA 
TACAATATGG GAAATATACC CACTCAAATC TCAGGGTCAA ATTACTACCT GTATCCTTCT 
GATGGGGGGC AGATTGAAGT GGAAAAGACA ATGTTTGAAA AAGGCCCAAC TTTTATCAAA 
GTGAGAGTGG TAAAGGACAA CCACAATTTT ATGATTCATT TAAATGACCC TAAGGAAATT 
GTGAAAAAGG AAGAGAAAGG GGATTATTAT TTAGAAGAGG AAGAAGAAGG AGATGAGGAA 
CAAAGTCTTG AAACGGAAGT ATCAGATGCA AAGAATAAAG CTTTCAGCAA GTTTGGATCT 
TTTTCTGCCA CCTTAGAAAA TGGAATCTGC CTCTCGATAA GTTACTATGG ATCAAATGGA 
ATGGCACCAG AAGATAAGGA TCCTGATTTA GAAACAATAT TGAATATCCC TTCAGCACTC 
ACTCCAACAG TGGTTCCTGT TATAGTGACC GTTCCTCAAA GCAAAGCTAA AGGGAAAATA 
AAAGGCAAAG AAAAACCCAA AGAATCCCTT AAAGAAGAAG AACACCCAAA AGAAGAAGAG 
AAAAAGGAAG AAGAAGTAGA ACCAGAACCT GTTTTACAAG AGACTTTGGA TGTTCCCACC 
TTCCAGAGCC TAAATGTGTC TTGCCCCAGT GGGCTCCTGT TGACTTTCAT TGGACAAGAA 
TCTACAGGTC AATATGTTAT AGATGAGGAA CCCACCTGGG ACATCATGGT CCGTCAGAGC 
TACCCCCAGA GGGTGAAGCA CTATGAGTTC TATAAAACGG TGATGCCACC CGCAGAGCAG 
GAGGCTTCAA GGGTTATCAC CAGTCAAGGC ACTGTTGTCA AATATATGTT GGATGGATCC 
ACACAGATTC TCTTTGCAGA TGGTGCTGTG AGCAGGAGTC CCAATTCAGG TCTTATTTGT 
CCTCCTTCTG AAATGCCAGC AACGCCTCAC AGTGGAGATT TGATGGACTC TATTTCTCAG 
CAGAAATCAG AAACGATACC ATCTGAGATT ACCAACACAA AGAAAGGAAA AAGTCACAAA 
AGTCAGTCAT CAATGGCCCA TAAGGGTGAA ATCCATGACC CTCCTCCAGA GGCAGTTCAA 
ACTGTAACTC CTGTGGAGGT TCACATAGGC ACCTGGTTTA CAACCACACC TGAAGGAAAT 
CGGATCGGCA CCAAAGGATT AGAAAGAATA GCAGACTTGA CCCCATTGTT ATCCTTTCAG 
GCCACAGATC CTGTCAATGG AACGGTTATG ACAACTCGAG AAGACAAAGT TGTCATAGTT 
GAAAGGAAAG ATGGTACTCG GATAGTGGAT CATGCTGATG GTACCAGAAT CACAACCTTT 
TATCAAGTTT ATGAAGATCA AATTATTCTG CCAGATGATC AAGAAACAAC CGAGGGTCCT 
CGGACTGTCA CCAGGCAGGT GAAGTGTATG CGGGTAGAAA GCTCACGCTA TGCCACTGTT 
ATCGCCAACT GTGAGGACAG TAGCTGCTGT GCCACCTTTG GAGATGGAAC AACTATTATT 
GCAAAGCCAC AGGGAACATA CCAGGTGTTA CCTCCAAACA CAGGCTCTCT TTATATTGAC 
AAGGATTGTT CAGCTGTGTA CTGCCATGAG TCAAGCAGTA ATATATACTA TCCTTTTCAA 
AAGCGTGAGC AGCTGCGAGC TGGCAGGTAC ATCATGAGGC ATACTTCAGA GGTTATCTGT 
GAGGTTCTGG ATCCTGAGGG AAACACTTTT CAGGTCATGG CTGATGGTAG CATATCAACT 
ATATTACCTG AAAAAAAATT GGAAGATGAT TTAAATGAGA AAACTGAGGG CTATGATAGT 
CTGTCCTCTA TGCACCTTGA AAAGAATCAT CAGCAAATCT ATGGTGAACA TGTCCCCAGG 
TTTTTTGTTA TGTATGCTGA TGGATCAGGA ATGGAACTTC TTCGAGACAG TGACATAGAA 
GAATATCTAT CTTTGGCATA TAAAGAATCA AATACTGTTG TTCTCCAAGA GCCAGTGCAG 
GAACAGCCAG GCACCCTAAC CATCACAGTC CTTCGCCCTT TCCATGAAGC ATCACCATGG 
CAAGTAAAAA AGGAAGATAC AATTGTCCCT CCTAATCTCC GGTCAAGGTC ATGGGAAACA 
TTTCCCTCAG TTGAGAAAAA AACTCCAGGA CCTCCGTTTG GTACTCAGAT TTGGAAAGGC 
CTTTGCATTG AGTCCAAACA GCTAGTGAGT GCCCCGGGTG CCATACTCAA GAGCCCCAGT 
GTGCTACAGA TGCGCCAATT CATTCAGCAT GAGGTCATAA AGAATGAGGT GAAACTGAGG 
CTGCAGGTTT CCCTTAAGGA TTACATAAAC TATATTCTAA AGAAAGAAGA TGAGCTGCAG 
GAAATGATGG TTAAAGATTC CAGAACTGAG GAGGAGAGAG GCAATGCTGC TGATCTCCTC 
AAGCTGGTTA TGTCTTTCCC TAAAATGGAG GAAACTACAA AAAGTCATGT TACTGAAGTT 
GCAGCTCACC TAACTGATTT ATTCAAGCAG TCTTTGGCTA CGCCTCCAAA ATGCCCACCA 
GACACATTTG GTAAAGATTT CTTTGAAAAG ACATGGAGAC ACACAGCATC CTCAAAACGC 
TGGAAAGAAA AGATAGACAA AACGAGGAAG GAAATTGAGA CAACACAGAA TTACCTAATG 
GATATTAAGA ACCGCATAAT ACCACCCTTT TTTAAATCTG AATTGAACCA GTTATATCAG 
TCTCAGTATA ATCACCTGGA CAGTCTTTCC AAAAAACTGC CTTCTTTTAC AAAGAAAAAT 
GAAGATGCAA ACGAAACAGC TGTTCAAGAT ACATCTGATC TTAATCTAGA TTTCAAGCCA 
CATAAGGTTT CAGAACAGAA ATCCTCAAGT GTGCCTAGTC TTCCAAAACC AGAGATTTCT 
GCAGATAAGA AGGATTTCAC TGCTCAGAAC CAAACTGAAA ATTTAACAAA ATCTCCTGAA 
GAAGCAGAAT CTTATGAGCC CGTGAAAATT CCAACCCAGT CCTTGCTGCA GGATGTTGCG 
GGACAAACAA GAAAAGAAAA AGTGAAGTTG CCTCATTATT TGCTGAGTTC CAAGCCTAAG 
TCTCAACCTC TTGCAAAGGT GCAAGATTCT GTTGGAGGAA AAGTGAACAC ATCCTCTGTT 
GCATCTGCTG CCATTAATAA TGCAAAGTCA TCCCTTTTTG GGTTCCATCT TCTCCCATCA 
TCAGTCAAGT TTGGAGTGCT TAAGGAAGGA CATACCTATG CCACAGTTGT AAAGCTCAAG 
AATGTTGGAG TGGACTTCTG CAGGTTTAAA GTAAAGCAGC CCCCACCCAG CACAGGACTG 
AAAGTGACTT ACAAACCTGG ACCTGTGGCA GCTGGTATGC AGACAGAACT GAATATAGAG 
TTATTTGCCA CAGCTGTTGG AGAGGATGGG GCCAAGGGAT CAGCACACAT CTCTCACAAT 
ATCGAGATTA TGACAGAGCA TGAGGTTCTG TTCCTACCTG TGGAAGCAAC TGTTTTAACA 
AGCAGCAATT ATGATAAACG ACCAAAAGAC TTTCCCCAGG GAAAAGAAAA TCCAATGGTC 
CAGAGAACTT CTACAATTTA TTCCTCCACA CTTGGAGTCT TCATGTCTCG TAAAGTTTCT 
CCACATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPAG17 ( XM_011540942.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011539244.1 Homo sapiens sperm associated antigen 17 (SPAG17), transcript variant X11, mRNA.

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CloneID	OHu77639
Clone ID Related Accession (Same CDS sequence)	XM_011540941.2 , XM_011540942.2
Accession Version	XM_011540942.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4389bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	sperm-associated antigen 17 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011540942.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGTGG AAGCAGATTT AGAGGACATA AAGAAAACAC AGCAGCGCAG TCTAATGGAC 
TGGAGTTTTA CTGAACATTT TAAACCGAAA GTACTGCTTC AGGTCCTTCA AGAAGCCCAT 
AAGCAATATA GGTGTGTTGA TTCTTACTAC CACACCCAAG ACAACTCTTT ACTTTTAGTC 
TTTCACAATC CAATGAATAG ACAACGTTTG CATTGTGAAT ATTGGAACAT TGCTCTCCAC 
TCCAATGTTG GATTCAGGAA TTATTTGGAA CTTGTTGCAA AATCTATTCA AGATTGGATT 
ACAAAAGAAG AAGCTATATA TCAGGAATCT AAAATGAATG AGAAAATCAT CAGGACCAGA 
GCTGAGCTGG AATTGAAATC TTCTGCTAAT GCCAAACTTA CTTCTGCTAG CAAAATTTTT 
TCCATTAAAG AATCTAAAAG TAACAAAGGA ATCAGCAAAA CAGAGATATC AGATCAAGAA 
AAAGAAAAAG AGAAGGAAAA GATTCCTTTC ATTTTAGAAG GCTCTCTCAA GGCATGGAAA 
GAAGAGCAAC ATCGATTAGC AGAAGAGGAG CGCTTAAGGG AAGAAAAGAA AGCAGAGAAG 
AAGGGTAAAG AAGCTGGTAA AAAGAAAGGC AAGGATAACG CAGAGAAAGA GGATAGTAGG 
TCTTTGAAGA AAAAATCACC TTACAAGGAG AAATCTAAAG AAGAACAAGT CAAGATCCAA 
GAAGTAACAG AAGAGTCCCC CCACCAACCA GAACCTAAGA TAACTTACCC GTTTCACGGA 
TACAATATGG GAAATATACC CACTCAAATC TCAGGGTCAA ATTACTACCT GTATCCTTCT 
GATGGGGGGC AGATTGAAGT GGAAAAGACA ATGTTTGAAA AAGGCCCAAC TTTTATCAAA 
GTGAGAGTGG TAAAGGACAA CCACAATTTT ATGATTCATT TAAATGACCC TAAGGAAATT 
GTGAAAAAGG AAGAGAAAGG GGATTATTAT TTAGAAGAGG AAGAAGAAGG AGATGAGGAA 
CAAAGTCTTG AAACGGAAGT ATCAGATGCA AAGAATAAAG CTTTCAGCAA GTTTGGATCT 
TTTTCTGCCA CCTTAGAAAA TGGAATCTGC CTCTCGATAA GTTACTATGG ATCAAATGGA 
ATGGCACCAG AAGATAAGGA TCCTGATTTA GAAACAATAT TGAATATCCC TTCAGCACTC 
ACTCCAACAG TGGTTCCTGT TATAGTGACC GTTCCTCAAA GCAAAGCTAA AGGGAAAATA 
AAAGGCAAAG AAAAACCCAA AGAATCCCTT AAAGAAGAAG AACACCCAAA AGAAGAAGAG 
AAAAAGGAAG AAGAAGTAGA ACCAGAACCT GTTTTACAAG AGACTTTGGA TGTTCCCACC 
TTCCAGAGCC TAAATGTGTC TTGCCCCAGT GGGCTCCTGT TGACTTTCAT TGGACAAGAA 
TCTACAGGTC AATATGTTAT AGATGAGGAA CCCACCTGGG ACATCATGGT CCGTCAGAGC 
TACCCCCAGA GGGTGAAGCA CTATGAGTTC TATAAAACGG TGATGCCACC CGCAGAGCAG 
GAGGCTTCAA GGGTTATCAC CAGTCAAGGC ACTGTTGTCA AATATATGTT GGATGGATCC 
ACACAGATTC TCTTTGCAGA TGGTGCTGTG AGCAGGAGTC CCAATTCAGG TCTTATTTGT 
CCTCCTTCTG AAATGCCAGC AACGCCTCAC AGTGGAGATT TGATGGACTC TATTTCTCAG 
CAGAAATCAG AAACGATACC ATCTGAGATT ACCAACACAA AGAAAGGAAA AAGTCACAAA 
AGTCAGTCAT CAATGGCCCA TAAGGGTGAA ATCCATGACC CTCCTCCAGA GGCAGTTCAA 
ACTGTAACTC CTGTGGAGGT TCACATAGGC ACCTGGTTTA CAACCACACC TGAAGGAAAT 
CGGATCGGCA CCAAAGGATT AGAAAGAATA GCAGACTTGA CCCCATTGTT ATCCTTTCAG 
GCCACAGATC CTGTCAATGG AACGGTTATG ACAACTCGAG AAGACAAAGT TGTCATAGTT 
GAAAGGAAAG ATGGTACTCG GATAGTGGAT CATGCTGATG GTACCAGAAT CACAACCTTT 
TATCAAGTTT ATGAAGATCA AATTATTCTG CCAGATGATC AAGAAACAAC CGAGGGTCCT 
CGGACTGTCA CCAGGCAGGT GAAGTGTATG CGGGTAGAAA GCTCACGCTA TGCCACTGTT 
ATCGCCAACT GTGAGGACAG TAGCTGCTGT GCCACCTTTG GAGATGGAAC AACTATTATT 
GCAAAGCCAC AGGGAACATA CCAGGTGTTA CCTCCAAACA CAGGCTCTCT TTATATTGAC 
AAGGATTGTT CAGCTGTGTA CTGCCATGAG TCAAGCAGTA ATATATACTA TCCTTTTCAA 
AAGCGTGAGC AGCTGCGAGC TGGCAGGTAC ATCATGAGGC ATACTTCAGA GGTTATCTGT 
GAGGTTCTGG ATCCTGAGGG AAACACTTTT CAGGTCATGG CTGATGGTAG CATATCAACT 
ATATTACCTG AAAAAAAATT GGAAGATGAT TTAAATGAGA AAACTGAGGG CTATGATAGT 
CTGTCCTCTA TGCACCTTGA AAAGAATCAT CAGCAAATCT ATGGTGAACA TGTCCCCAGG 
TTTTTTGTTA TGTATGCTGA TGGATCAGGA ATGGAACTTC TTCGAGACAG TGACATAGAA 
GAATATCTAT CTTTGGCATA TAAAGAATCA AATACTGTTG TTCTCCAAGA GCCAGTGCAG 
GAACAGCCAG GCACCCTAAC CATCACAGTC CTTCGCCCTT TCCATGAAGC ATCACCATGG 
CAAGTAAAAA AGGAAGATAC AATTGTCCCT CCTAATCTCC GGTCAAGGTC ATGGGAAACA 
TTTCCCTCAG TTGAGAAAAA AACTCCAGGA CCTCCGTTTG GTACTCAGAT TTGGAAAGGC 
CTTTGCATTG AGTCCAAACA GCTAGTGAGT GCCCCGGGTG CCATACTCAA GAGCCCCAGT 
GTGCTACAGA TGCGCCAATT CATTCAGCAT GAGGTCATAA AGAATGAGGT GAAACTGAGG 
CTGCAGGTTT CCCTTAAGGA TTACATAAAC TATATTCTAA AGAAAGAAGA TGAGCTGCAG 
GAAATGATGG TTAAAGATTC CAGAACTGAG GAGGAGAGAG GCAATGCTGC TGATCTCCTC 
AAGCTGGTTA TGTCTTTCCC TAAAATGGAG GAAACTACAA AAAGTCATGT TACTGAAGTT 
GCAGCTCACC TAACTGATTT ATTCAAGCAG TCTTTGGCTA CGCCTCCAAA ATGCCCACCA 
GACACATTTG GTAAAGATTT CTTTGAAAAG ACATGGAGAC ACACAGCATC CTCAAAACGC 
TGGAAAGAAA AGATAGACAA AACGAGGAAG GAAATTGAGA CAACACAGAA TTACCTAATG 
GATATTAAGA ACCGCATAAT ACCACCCTTT TTTAAATCTG AATTGAACCA GTTATATCAG 
TCTCAGTATA ATCACCTGGA CAGTCTTTCC AAAAAACTGC CTTCTTTTAC AAAGAAAAAT 
GAAGATGCAA ACGAAACAGC TGTTCAAGAT ACATCTGATC TTAATCTAGA TTTCAAGCCA 
CATAAGGTTT CAGAACAGAA ATCCTCAAGT GTGCCTAGTC TTCCAAAACC AGAGATTTCT 
GCAGATAAGA AGGATTTCAC TGCTCAGAAC CAAACTGAAA ATTTAACAAA ATCTCCTGAA 
GAAGCAGAAT CTTATGAGCC CGTGAAAATT CCAACCCAGT CCTTGCTGCA GGATGTTGCG 
GGACAAACAA GAAAAGAAAA AGTGAAGTTG CCTCATTATT TGCTGAGTTC CAAGCCTAAG 
TCTCAACCTC TTGCAAAGGT GCAAGATTCT GTTGGAGGAA AAGTGAACAC ATCCTCTGTT 
GCATCTGCTG CCATTAATAA TGCAAAGTCA TCCCTTTTTG GGTTCCATCT TCTCCCATCA 
TCAGTCAAGT TTGGAGTGCT TAAGGAAGGA CATACCTATG CCACAGTTGT AAAGCTCAAG 
AATGTTGGAG TGGACTTCTG CAGGTTTAAA GTAAAGCAGC CCCCACCCAG CACAGGACTG 
AAAGTGACTT ACAAACCTGG ACCTGTGGCA GCTGGTATGC AGACAGAACT GAATATAGAG 
TTATTTGCCA CAGCTGTTGG AGAGGATGGG GCCAAGGGAT CAGCACACAT CTCTCACAAT 
ATCGAGATTA TGACAGAGCA TGAGGTTCTG TTCCTACCTG TGGAAGCAAC TGTTTTAACA 
AGCAGCAATT ATGATAAACG ACCAAAAGAC TTTCCCCAGG GAAAAGAAAA TCCAATGGTC 
CAGAGAACTT CTACAATTTA TTCCTCCACA CTTGGAGTCT TCATGTCTCG TAAAGTTTCT 
CCACATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011539244.1
CDS	279..4667
Translation	MMVEADLEDIKKTQQRSLMDWSFTEHFKPKVLLQVLQEAHKQYRCVDSYYHTQDNSLLLVFHNPMNRQRLHCEYWNIALHSNVGFRNYLELVAKSIQDWITKEEAIYQESKMNEKIIRTRAELELKSSANAKLTSASKIFSIKESKSNKGISKTEISDQEKEKEKEKIPFILEGSLKAWKEEQHRLAEEERLREEKKAEKKGKEAGKKKGKDNAEKEDSRSLKKKSPYKEKSKEEQVKIQEVTEESPHQPEPKITYPFHGYNMGNIPTQISGSNYYLYPSDGGQIEVEKTMFEKGPTFIKVRVVKDNHNFMIHLNDPKEIVKKEEKGDYYLEEEEEGDEEQSLETEVSDAKNKAFSKFGSFSATLENGICLSISYYGSNGMAPEDKDPDLETILNIPSALTPTVVPVIVTVPQSKAKGKIKGKEKPKESLKEEEHPKEEEKKEEEVEPEPVLQETLDVPTFQSLNVSCPSGLLLTFIGQESTGQYVIDEEPTWDIMVRQSYPQRVKHYEFYKTVMPPAEQEASRVITSQGTVVKYMLDGSTQILFADGAVSRSPNSGLICPPSEMPATPHSGDLMDSISQQKSETIPSEITNTKKGKSHKSQSSMAHKGEIHDPPPEAVQTVTPVEVHIGTWFTTTPEGNRIGTKGLERIADLTPLLSFQATDPVNGTVMTTREDKVVIVERKDGTRIVDHADGTRITTFYQVYEDQIILPDDQETTEGPRTVTRQVKCMRVESSRYATVIANCEDSSCCATFGDGTTIIAKPQGTYQVLPPNTGSLYIDKDCSAVYCHESSSNIYYPFQKREQLRAGRYIMRHTSEVICEVLDPEGNTFQVMADGSISTILPEKKLEDDLNEKTEGYDSLSSMHLEKNHQQIYGEHVPRFFVMYADGSGMELLRDSDIEEYLSLAYKESNTVVLQEPVQEQPGTLTITVLRPFHEASPWQVKKEDTIVPPNLRSRSWETFPSVEKKTPGPPFGTQIWKGLCIESKQLVSAPGAILKSPSVLQMRQFIQHEVIKNEVKLRLQVSLKDYINYILKKEDELQEMMVKDSRTEEERGNAADLLKLVMSFPKMEETTKSHVTEVAAHLTDLFKQSLATPPKCPPDTFGKDFFEKTWRHTASSKRWKEKIDKTRKEIETTQNYLMDIKNRIIPPFFKSELNQLYQSQYNHLDSLSKKLPSFTKKNEDANETAVQDTSDLNLDFKPHKVSEQKSSSVPSLPKPEISADKKDFTAQNQTENLTKSPEEAESYEPVKIPTQSLLQDVAGQTRKEKVKLPHYLLSSKPKSQPLAKVQDSVGGKVNTSSVASAAINNAKSSLFGFHLLPSSVKFGVLKEGHTYATVVKLKNVGVDFCRFKVKQPPPSTGLKVTYKPGPVAAGMQTELNIELFATAVGEDGAKGSAHISHNIEIMTEHEVLFLPVEATVLTSSNYDKRPKDFPQGKENPMVQRTSTIYSSTLGVFMSRKVSPH

Target ORF information:

RefSeq Version	XM_011540942.2
Organism	Homo sapiens(human)
Definition	Homo sapiens sperm associated antigen 17 (SPAG17), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011540942.2

ORF Insert Sequence:

ATGATGGTGG AAGCAGATTT AGAGGACATA AAGAAAACAC AGCAGCGCAG TCTAATGGAC 
TGGAGTTTTA CTGAACATTT TAAACCGAAA GTACTGCTTC AGGTCCTTCA AGAAGCCCAT 
AAGCAATATA GGTGTGTTGA TTCTTACTAC CACACCCAAG ACAACTCTTT ACTTTTAGTC 
TTTCACAATC CAATGAATAG ACAACGTTTG CATTGTGAAT ATTGGAACAT TGCTCTCCAC 
TCCAATGTTG GATTCAGGAA TTATTTGGAA CTTGTTGCAA AATCTATTCA AGATTGGATT 
ACAAAAGAAG AAGCTATATA TCAGGAATCT AAAATGAATG AGAAAATCAT CAGGACCAGA 
GCTGAGCTGG AATTGAAATC TTCTGCTAAT GCCAAACTTA CTTCTGCTAG CAAAATTTTT 
TCCATTAAAG AATCTAAAAG TAACAAAGGA ATCAGCAAAA CAGAGATATC AGATCAAGAA 
AAAGAAAAAG AGAAGGAAAA GATTCCTTTC ATTTTAGAAG GCTCTCTCAA GGCATGGAAA 
GAAGAGCAAC ATCGATTAGC AGAAGAGGAG CGCTTAAGGG AAGAAAAGAA AGCAGAGAAG 
AAGGGTAAAG AAGCTGGTAA AAAGAAAGGC AAGGATAACG CAGAGAAAGA GGATAGTAGG 
TCTTTGAAGA AAAAATCACC TTACAAGGAG AAATCTAAAG AAGAACAAGT CAAGATCCAA 
GAAGTAACAG AAGAGTCCCC CCACCAACCA GAACCTAAGA TAACTTACCC GTTTCACGGA 
TACAATATGG GAAATATACC CACTCAAATC TCAGGGTCAA ATTACTACCT GTATCCTTCT 
GATGGGGGGC AGATTGAAGT GGAAAAGACA ATGTTTGAAA AAGGCCCAAC TTTTATCAAA 
GTGAGAGTGG TAAAGGACAA CCACAATTTT ATGATTCATT TAAATGACCC TAAGGAAATT 
GTGAAAAAGG AAGAGAAAGG GGATTATTAT TTAGAAGAGG AAGAAGAAGG AGATGAGGAA 
CAAAGTCTTG AAACGGAAGT ATCAGATGCA AAGAATAAAG CTTTCAGCAA GTTTGGATCT 
TTTTCTGCCA CCTTAGAAAA TGGAATCTGC CTCTCGATAA GTTACTATGG ATCAAATGGA 
ATGGCACCAG AAGATAAGGA TCCTGATTTA GAAACAATAT TGAATATCCC TTCAGCACTC 
ACTCCAACAG TGGTTCCTGT TATAGTGACC GTTCCTCAAA GCAAAGCTAA AGGGAAAATA 
AAAGGCAAAG AAAAACCCAA AGAATCCCTT AAAGAAGAAG AACACCCAAA AGAAGAAGAG 
AAAAAGGAAG AAGAAGTAGA ACCAGAACCT GTTTTACAAG AGACTTTGGA TGTTCCCACC 
TTCCAGAGCC TAAATGTGTC TTGCCCCAGT GGGCTCCTGT TGACTTTCAT TGGACAAGAA 
TCTACAGGTC AATATGTTAT AGATGAGGAA CCCACCTGGG ACATCATGGT CCGTCAGAGC 
TACCCCCAGA GGGTGAAGCA CTATGAGTTC TATAAAACGG TGATGCCACC CGCAGAGCAG 
GAGGCTTCAA GGGTTATCAC CAGTCAAGGC ACTGTTGTCA AATATATGTT GGATGGATCC 
ACACAGATTC TCTTTGCAGA TGGTGCTGTG AGCAGGAGTC CCAATTCAGG TCTTATTTGT 
CCTCCTTCTG AAATGCCAGC AACGCCTCAC AGTGGAGATT TGATGGACTC TATTTCTCAG 
CAGAAATCAG AAACGATACC ATCTGAGATT ACCAACACAA AGAAAGGAAA AAGTCACAAA 
AGTCAGTCAT CAATGGCCCA TAAGGGTGAA ATCCATGACC CTCCTCCAGA GGCAGTTCAA 
ACTGTAACTC CTGTGGAGGT TCACATAGGC ACCTGGTTTA CAACCACACC TGAAGGAAAT 
CGGATCGGCA CCAAAGGATT AGAAAGAATA GCAGACTTGA CCCCATTGTT ATCCTTTCAG 
GCCACAGATC CTGTCAATGG AACGGTTATG ACAACTCGAG AAGACAAAGT TGTCATAGTT 
GAAAGGAAAG ATGGTACTCG GATAGTGGAT CATGCTGATG GTACCAGAAT CACAACCTTT 
TATCAAGTTT ATGAAGATCA AATTATTCTG CCAGATGATC AAGAAACAAC CGAGGGTCCT 
CGGACTGTCA CCAGGCAGGT GAAGTGTATG CGGGTAGAAA GCTCACGCTA TGCCACTGTT 
ATCGCCAACT GTGAGGACAG TAGCTGCTGT GCCACCTTTG GAGATGGAAC AACTATTATT 
GCAAAGCCAC AGGGAACATA CCAGGTGTTA CCTCCAAACA CAGGCTCTCT TTATATTGAC 
AAGGATTGTT CAGCTGTGTA CTGCCATGAG TCAAGCAGTA ATATATACTA TCCTTTTCAA 
AAGCGTGAGC AGCTGCGAGC TGGCAGGTAC ATCATGAGGC ATACTTCAGA GGTTATCTGT 
GAGGTTCTGG ATCCTGAGGG AAACACTTTT CAGGTCATGG CTGATGGTAG CATATCAACT 
ATATTACCTG AAAAAAAATT GGAAGATGAT TTAAATGAGA AAACTGAGGG CTATGATAGT 
CTGTCCTCTA TGCACCTTGA AAAGAATCAT CAGCAAATCT ATGGTGAACA TGTCCCCAGG 
TTTTTTGTTA TGTATGCTGA TGGATCAGGA ATGGAACTTC TTCGAGACAG TGACATAGAA 
GAATATCTAT CTTTGGCATA TAAAGAATCA AATACTGTTG TTCTCCAAGA GCCAGTGCAG 
GAACAGCCAG GCACCCTAAC CATCACAGTC CTTCGCCCTT TCCATGAAGC ATCACCATGG 
CAAGTAAAAA AGGAAGATAC AATTGTCCCT CCTAATCTCC GGTCAAGGTC ATGGGAAACA 
TTTCCCTCAG TTGAGAAAAA AACTCCAGGA CCTCCGTTTG GTACTCAGAT TTGGAAAGGC 
CTTTGCATTG AGTCCAAACA GCTAGTGAGT GCCCCGGGTG CCATACTCAA GAGCCCCAGT 
GTGCTACAGA TGCGCCAATT CATTCAGCAT GAGGTCATAA AGAATGAGGT GAAACTGAGG 
CTGCAGGTTT CCCTTAAGGA TTACATAAAC TATATTCTAA AGAAAGAAGA TGAGCTGCAG 
GAAATGATGG TTAAAGATTC CAGAACTGAG GAGGAGAGAG GCAATGCTGC TGATCTCCTC 
AAGCTGGTTA TGTCTTTCCC TAAAATGGAG GAAACTACAA AAAGTCATGT TACTGAAGTT 
GCAGCTCACC TAACTGATTT ATTCAAGCAG TCTTTGGCTA CGCCTCCAAA ATGCCCACCA 
GACACATTTG GTAAAGATTT CTTTGAAAAG ACATGGAGAC ACACAGCATC CTCAAAACGC 
TGGAAAGAAA AGATAGACAA AACGAGGAAG GAAATTGAGA CAACACAGAA TTACCTAATG 
GATATTAAGA ACCGCATAAT ACCACCCTTT TTTAAATCTG AATTGAACCA GTTATATCAG 
TCTCAGTATA ATCACCTGGA CAGTCTTTCC AAAAAACTGC CTTCTTTTAC AAAGAAAAAT 
GAAGATGCAA ACGAAACAGC TGTTCAAGAT ACATCTGATC TTAATCTAGA TTTCAAGCCA 
CATAAGGTTT CAGAACAGAA ATCCTCAAGT GTGCCTAGTC TTCCAAAACC AGAGATTTCT 
GCAGATAAGA AGGATTTCAC TGCTCAGAAC CAAACTGAAA ATTTAACAAA ATCTCCTGAA 
GAAGCAGAAT CTTATGAGCC CGTGAAAATT CCAACCCAGT CCTTGCTGCA GGATGTTGCG 
GGACAAACAA GAAAAGAAAA AGTGAAGTTG CCTCATTATT TGCTGAGTTC CAAGCCTAAG 
TCTCAACCTC TTGCAAAGGT GCAAGATTCT GTTGGAGGAA AAGTGAACAC ATCCTCTGTT 
GCATCTGCTG CCATTAATAA TGCAAAGTCA TCCCTTTTTG GGTTCCATCT TCTCCCATCA 
TCAGTCAAGT TTGGAGTGCT TAAGGAAGGA CATACCTATG CCACAGTTGT AAAGCTCAAG 
AATGTTGGAG TGGACTTCTG CAGGTTTAAA GTAAAGCAGC CCCCACCCAG CACAGGACTG 
AAAGTGACTT ACAAACCTGG ACCTGTGGCA GCTGGTATGC AGACAGAACT GAATATAGAG 
TTATTTGCCA CAGCTGTTGG AGAGGATGGG GCCAAGGGAT CAGCACACAT CTCTCACAAT 
ATCGAGATTA TGACAGAGCA TGAGGTTCTG TTCCTACCTG TGGAAGCAAC TGTTTTAACA 
AGCAGCAATT ATGATAAACG ACCAAAAGAC TTTCCCCAGG GAAAAGAAAA TCCAATGGTC 
CAGAGAACTT CTACAATTTA TTCCTCCACA CTTGGAGTCT TCATGTCTCG TAAAGTTTCT 
CCACATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPAG17 ( XM_006710426.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_006710489.1 Homo sapiens sperm associated antigen 17 (SPAG17), transcript variant X2, mRNA.

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CloneID	OHu33131
Clone ID Related Accession (Same CDS sequence)	XM_006710426.3
Accession Version	XM_006710426.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6558bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	sperm-associated antigen 17 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006710426.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCACCCA AGAAGGAGAA AGGAGGAACT GTGAACACCA GTTCTAAGAT ATGGGAACCC 
TCGCTCATAG CTGCACAGTT CAATCAGAAC GATTGGCAGG CCTCCATTGC TTTTGTGGTT 
GGGAACCAGA TTGAAGATGA TCTTCTCATC CAAGCCCTTA CCGTGGCTGT CCAGGTCCCT 
CAGCGTAAAC TCTTCAGTAT GGTGTCGTGG CAAGACATTC TCCAGCAGAT TAATGAAATA 
AATACACTTG TTGGATCTGC TTCATCTAAA AAGGCAAAAA AACCTGTAGG TGGTAATGCT 
CCTTTATATT ATGAGGTGTT AACGGCAGCA AAAGCAATTA TGGATAGTGG AGAGAAATTA 
ACCTTACCAC TGATAGGGAA ACTCTTGAAA TTTCAACTTC TCCAGATTAA ATTTAAGGAC 
CAACAGCGAC GGGAAAATGA AAAGAAGGTA ATAGAAGACA AACCTAAGTT AGAAAAGGAT 
AAAGGGAAAG CAAAATCTCC CAAGGAGAAA AAGGCTCCAA GTGCCAAGCC TGCCAAAGGA 
AAGGGAAAGG ATCAGCCTGA GGCAAATGCA CCAGTGAAAA AGACCACCCA GTTAAAGCGG 
AGAGGAGAAG ACGACCACAC CAATCGTTAC ATTGACGATG AGCCAGATGA TGGTGCCCAA 
CATTACATTA TAGTTGTGGG CTTTAACAAT CCTCAGCTAT TAGCAATTAT GGCTGAGCTT 
GGCATTCCTA TAACCAGCGT GATTAAAATA TCTTCAGAGA ATTATGAACC TCTGCAGACA 
CACCTGGCAG CAGTTAACCA GCAGCAGGAA GTTCTTCTTC AGTCAGAAGA TCTAGAAGCA 
GAAAAATTGA AGAAAGAAAA TGCCATAAAA GAGCTTAAAA CTTTCTGGAA GTACTTGGAA 
CCAGTCCTGA ATAATGAGAA ACCTGAAACA AATCTCTTTG ATGTTGCTCG ACTTGAGTAC 
ATGGTCAAAG CAGCTGATTT TCCTTCTGAC TGGTCAGATG GTGAGATGAT GCTGAAATTG 
GGCACTGATA TTTTTGAAAA TATTGCCTGC TTGATGTATG ACATCCTGGA TTGGAAAAGG 
CAGCACCAGC ACTATTTGGA AAGCATGCAG CTTATTAATG TTCCACAAGT GGTTAATGAG 
AAACCTGTAT TAGAAGCCAT GCCAACTTCA GAGGCTCCAC AACCTGCTGT ACCAGCTCCT 
GGAAAGAAGA AAGCACAGTA TGAAGAACCG CAAGCTCCAC CACCAGTGAC TTCAGTCATC 
ACAACTGAAG TAGACATGAG ATATTACAAT TATTTGCTGA ATCCAATTCG AGAGGAATTC 
ATTTCTGTGC CCCTGATACT GCATTGTATG CTGGAACAGG TTGTTGCAAC TGAAGAAGAT 
CTCGTCCCAC CCAGTCTGCG GGAGCCATCC CCCAGAGCAG ACGGGCTAGA CCACAGAATC 
GCAGCTCACA TTGTGTCCCT TCTGCCCTCA CTCTGTCTCT CAGAGAGGGA GAAAAAGAAT 
CTTCATGACA TATTTTTATC TGAAGAAGAA AATGAAAGCA AAGCAGTGCC CAAAGGCCCC 
CTCCTACTGA ACTATCATGA TGCACACGCC CACAAGAAGT ACGCACTACA GGACCAAAAG 
AATTTTGATC CAGTTCAAAT TGAGCAGGAG ATGCAGTCCA AGTTGCCACT GTGGGAATTT 
CTTCAATTCC CTCTACCCCC ACCATGGAAC AACACTAAAC GTCTAGCTAC AATTCATGAG 
CTTATGCACT TTTGTACGAG TGATGTCTTG AGCTGGAATG AAGTAGAACG AGCCTTCAAG 
GTGTTTACTT TTGAGAGCCT GAAGCTCTCT GAGGTTGATG AAAAAGGGAA ACTGAAACCT 
TCTGGGATGA TGTGTGGGTC AGATTCTGAA ATGTTCAACA TACCGTGGGA CAACCCTGCC 
AGATTTGCTA AACAGATAAG GCAGCAATAT GTCATGAAAA TGAATACTCA AGAGGCCAAG 
CAGAAAGCAG ATATTAAAAT CAAAGACAGA ACACTATTTG TGGATCAGAA TTTGTCAATG 
TCTGTGCAAG ATAATGAAAG CAACCGAGAA CCTTCAGATC CTAGTCAGTG TGATGCTAAC 
AATATGAAGC ATTCTGACTT GAATAATCTC AAACTCTCAG TCCCTGATAA TAGACAGCTG 
TTAGAGCAGG AGAGCATCAT GAAGGCTCAG CCCCAACATG AGTCTCTGGA GCAGACCACA 
AACAATGAGA TCAAAGATGA TGCAGTCACA AAGGCTGATT CTCATGAAAA GAAACCCAAG 
AAGATGATGG TGGAAGCAGA TTTAGAGGAC ATAAAGAAAA CACAGCAGCG CAGTCTAATG 
GACTGGAGTT TTACTGAACA TTTTAAACCG AAAGTACTGC TTCAGGTCCT TCAAGAAGCC 
CATAAGCAAT ATAGGTGTGT TGATTCTTAC TACCACACCC AAGACAACTC TTTACTTTTA 
GTCTTTCACA ATCCAATGAA TAGACAACGT TTGCATTGTG AATATTGGAA CATTGCTCTC 
CACTCCAATG TTGGATTCAG GAATTATTTG GAACTTGTTG CAAAATCTAT TCAAGATTGG 
ATTACAAAAG AAGAAGCTAT ATATCAGGAA TCTAAAATGA ATGAGAAAAT CATCAGGACC 
AGAGCTGAGC TGGAATTGAA ATCTTCTGCT AATGCCAAAC TTACTTCTGC TAGCAAAATT 
TTTTCCATTA AAGAATCTAA AAGTAACAAA GGAATCAGCA AAACAGAGAT ATCAGATCAA 
GAAAAAGAAA AAGAGAAGGA AAAGATTCCT TTCATTTTAG AAGGCTCTCT CAAGGCATGG 
AAAGAAGAGC AACATCGATT AGCAGAAGAG GAGCGCTTAA GGGAAGAAAA GAAAGCAGAG 
AAGAAGGGTA AAGAAGCTGG TAAAAAGAAA GGCAAGGATA ACGCAGAGAA AGAGGATAGT 
AGGTCTTTGA AGAAAAAATC ACCTTACAAG GAGAAATCTA AAGAAGAACA AGTCAAGATC 
CAAGAAGTAA CAGAAGAGTC CCCCCACCAA CCAGAACCTA AGATAACTTA CCCGTTTCAC 
GGATACAATA TGGGAAATAT ACCCACTCAA ATCTCAGGGT CAAATTACTA CCTGTATCCT 
TCTGATGGGG GGCAGATTGA AGTGGAAAAG ACAATGTTTG AAAAAGGCCC AACTTTTATC 
AAAGTGAGAG TGGTAAAGGA CAACCACAAT TTTATGATTC ATTTAAATGA CCCTAAGGAA 
ATTGTGAAAA AGGAAGAGAA AGGGGATTAT TATTTAGAAG AGGAAGAAGA AGGAGATGAG 
GAACAAAGTC TTGAAACGGA AGATAAGGAT CCTGATTTAG AAACAATATT GAATATCCCT 
TCAGCACTCA CTCCAACAGT GGTTCCTGTT ATAGTGACCG TTCCTCAAAG CAAAGCTAAA 
GGGAAAATAA AAGGCAAAGA AAAACCCAAA GAATCCCTTA AAGAAGAAGA ACACCCAAAA 
GAAGAAGAGA AAAAGGAAGA AGAAGTAGAA CCAGAACCTG TTTTACAAGA GACTTTGGAT 
GTTCCCACCT TCCAGAGCCT AAATGTGTCT TGCCCCAGTG GGCTCCTGTT GACTTTCATT 
GGACAAGAAT CTACAGGTCA ATATGTTATA GATGAGGAAC CCACCTGGGA CATCATGGTC 
CGTCAGAGCT ACCCCCAGAG GGTGAAGCAC TATGAGTTCT ATAAAACGGT GATGCCACCC 
GCAGAGCAGG AGGCTTCAAG GGTTATCACC AGTCAAGGCA CTGTTGTCAA ATATATGTTG 
GATGGATCCA CACAGATTCT CTTTGCAGAT GGTGCTGTGA GCAGGAGTCC CAATTCAGGT 
CTTATTTGTC CTCCTTCTGA AATGCCAGCA ACGCCTCACA GTGGAGATTT GATGGACTCT 
ATTTCTCAGC AGAAATCAGA AACGATACCA TCTGAGATTA CCAACACAAA GAAAGGAAAA 
AGTCACAAAA GTCAGTCATC AATGGCCCAT AAGGGTGAAA TCCATGACCC TCCTCCAGAG 
GCAGTTCAAA CTGTAACTCC TGTGGAGGTT CACATAGGCA CCTGGTTTAC AACCACACCT 
GAAGGAAATC GGATCGGCAC CAAAGGATTA GAAAGAATAG CAGACTTGAC CCCATTGTTA 
TCCTTTCAGG CCACAGATCC TGTCAATGGA ACGGTTATGA CAACTCGAGA AGACAAAGTT 
GTCATAGTTG AAAGGAAAGA TGGTACTCGG ATAGTGGATC ATGCTGATGG TACCAGAATC 
ACAACCTTTT ATCAAGTTTA TGAAGATCAA ATTATTCTGC CAGATGATCA AGAAACAACC 
GAGGGTCCTC GGACTGTCAC CAGGCAGGTG AAGTGTATGC GGGTAGAAAG CTCACGCTAT 
GCCACTGTTA TCGCCAACTG TGAGGACAGT AGCTGCTGTG CCACCTTTGG AGATGGAACA 
ACTATTATTG CAAAGCCACA GGGAACATAC CAGGTGTTAC CTCCAAACAC AGGCTCTCTT 
TATATTGACA AGGATTGTTC AGCTGTGTAC TGCCATGAGT CAAGCAGTAA TATATACTAT 
CCTTTTCAAA AGCGTGAGCA GCTGCGAGCT GGCAGGTACA TCATGAGGCA TACTTCAGAG 
GTTATCTGTG AGGTTCTGGA TCCTGAGGGA AACACTTTTC AGGTCATGGC TGATGGTAGC 
ATATCAACTA TATTACCTGA AAAAAAATTG GAAGATGATT TAAATGAGAA AACTGAGGGC 
TATGATAGTC TGTCCTCTAT GCACCTTGAA AAGAATCATC AGCAAATCTA TGGTGAACAT 
GTCCCCAGGT TTTTTGTTAT GTATGCTGAT GGATCAGGAA TGGAACTTCT TCGAGACAGT 
GACATAGAAG AATATCTATC TTTGGCATAT AAAGAATCAA ATACTGTTGT TCTCCAAGAG 
CCAGTGCAGG AACAGCCAGG CACCCTAACC ATCACAGTCC TTCGCCCTTT CCATGAAGCA 
TCACCATGGC AAGTAAAAAA GGAAGATACA ATTGTCCCTC CTAATCTCCG GTCAAGGTCA 
TGGGAAACAT TTCCCTCAGT TGAGAAAAAA ACTCCAGGAC CTCCGTTTGG TACTCAGATT 
TGGAAAGGCC TTTGCATTGA GTCCAAACAG CTAGTGAGTG CCCCGGGTGC CATACTCAAG 
AGCCCCAGTG TGCTACAGAT GCGCCAATTC ATTCAGCATG AGGTCATAAA GAATGAGGTG 
AAACTGAGGC TGCAGGTTTC CCTTAAGGAT TACATAAACT ATATTCTAAA GAAAGAAGAT 
GAGCTGCAGG AAATGATGGT TAAAGATTCC AGAACTGAGG AGGAGAGAGG CAATGCTGCT 
GATCTCCTCA AGCTGGTTAT GTCTTTCCCT AAAATGGAGG AAACTACAAA AAGTCATGTT 
ACTGAAGTTG CAGCTCACCT AACTGATTTA TTCAAGCAGT CTTTGGCTAC GCCTCCAAAA 
TGCCCACCAG ACACATTTGG TAAAGATTTC TTTGAAAAGA CATGGAGACA CACAGCATCC 
TCAAAACGCT GGAAAGAAAA GATAGACAAA ACGAGGAAGG AAATTGAGAC AACACAGAAT 
TACCTAATGG ATATTAAGAA CCGCATAATA CCACCCTTTT TTAAATCTGA ATTGAACCAG 
TTATATCAGT CTCAGTATAA TCACCTGGAC AGTCTTTCCA AAAAACTGCC TTCTTTTACA 
AAGAAAAATG AAGATGCAAA CGAAACAGCT GTTCAAGATA CATCTGATCT TAATCTAGAT 
TTCAAGCCAC ATAAGGTTTC AGAACAGAAA TCCTCAAGTG TGCCTAGTCT TCCAAAACCA 
GAGATTTCTG CAGATAAGAA GGATTTCACT GCTCAGAACC AAACTGAAAA TTTAACAAAA 
TCTCCTGAAG AAGCAGAATC TTATGAGCCC GTGAAAATTC CAACCCAGTC CTTGCTGCAG 
GATGTTGCGG GACAAACAAG AAAAGAAAAA GTGAAGTTGC CTCATTATTT GCTGAGTTCC 
AAGCCTAAGT CTCAACCTCT TGCAAAGGTG CAAGATTCTG TTGGAGGAAA AGTGAACACA 
TCCTCTGTTG CATCTGCTGC CATTAATAAT GCAAAGTCAT CCCTTTTTGG GTTCCATCTT 
CTCCCATCAT CAGTCAAGTT TGGAGTGCTT AAGGAAGGAC ATACCTATGC CACAGTTGTA 
AAGCTCAAGA ATGTTGGAGT GGACTTCTGC AGGTTTAAAG TAAAGCAGCC CCCACCCAGC 
ACAGGACTGA AAGTGACTTA CAAACCTGGA CCTGTGGCAG CTGGTATGCA GACAGAACTG 
AATATAGAGT TATTTGCCAC AGCTGTTGGA GAGGATGGGG CCAAGGGATC AGCACACATC 
TCTCACAATA TCGAGATTAT GACAGAGCAT GAGGTTCTGT TCCTACCTGT GGAAGCAACT 
GTTTTAACAA GCAGCAATTA TGATAAACGA CCAAAAGACT TTCCCCAGGG AAAAGAAAAT 
CCAATGGTCC AGAGAACTTC TACAATTTAT TCCTCCACAC TTGGAGTCTT CATGTCTCGT 
AAAGTTTCTC CACATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006710489.1
CDS	69..6626
Translation	MAPKKEKGGTVNTSSKIWEPSLIAAQFNQNDWQASIAFVVGNQIEDDLLIQALTVAVQVPQRKLFSMVSWQDILQQINEINTLVGSASSKKAKKPVGGNAPLYYEVLTAAKAIMDSGEKLTLPLIGKLLKFQLLQIKFKDQQRRENEKKVIEDKPKLEKDKGKAKSPKEKKAPSAKPAKGKGKDQPEANAPVKKTTQLKRRGEDDHTNRYIDDEPDDGAQHYIIVVGFNNPQLLAIMAELGIPITSVIKISSENYEPLQTHLAAVNQQQEVLLQSEDLEAEKLKKENAIKELKTFWKYLEPVLNNEKPETNLFDVARLEYMVKAADFPSDWSDGEMMLKLGTDIFENIACLMYDILDWKRQHQHYLESMQLINVPQVVNEKPVLEAMPTSEAPQPAVPAPGKKKAQYEEPQAPPPVTSVITTEVDMRYYNYLLNPIREEFISVPLILHCMLEQVVATEEDLVPPSLREPSPRADGLDHRIAAHIVSLLPSLCLSEREKKNLHDIFLSEEENESKAVPKGPLLLNYHDAHAHKKYALQDQKNFDPVQIEQEMQSKLPLWEFLQFPLPPPWNNTKRLATIHELMHFCTSDVLSWNEVERAFKVFTFESLKLSEVDEKGKLKPSGMMCGSDSEMFNIPWDNPARFAKQIRQQYVMKMNTQEAKQKADIKIKDRTLFVDQNLSMSVQDNESNREPSDPSQCDANNMKHSDLNNLKLSVPDNRQLLEQESIMKAQPQHESLEQTTNNEIKDDAVTKADSHEKKPKKMMVEADLEDIKKTQQRSLMDWSFTEHFKPKVLLQVLQEAHKQYRCVDSYYHTQDNSLLLVFHNPMNRQRLHCEYWNIALHSNVGFRNYLELVAKSIQDWITKEEAIYQESKMNEKIIRTRAELELKSSANAKLTSASKIFSIKESKSNKGISKTEISDQEKEKEKEKIPFILEGSLKAWKEEQHRLAEEERLREEKKAEKKGKEAGKKKGKDNAEKEDSRSLKKKSPYKEKSKEEQVKIQEVTEESPHQPEPKITYPFHGYNMGNIPTQISGSNYYLYPSDGGQIEVEKTMFEKGPTFIKVRVVKDNHNFMIHLNDPKEIVKKEEKGDYYLEEEEEGDEEQSLETEDKDPDLETILNIPSALTPTVVPVIVTVPQSKAKGKIKGKEKPKESLKEEEHPKEEEKKEEEVEPEPVLQETLDVPTFQSLNVSCPSGLLLTFIGQESTGQYVIDEEPTWDIMVRQSYPQRVKHYEFYKTVMPPAEQEASRVITSQGTVVKYMLDGSTQILFADGAVSRSPNSGLICPPSEMPATPHSGDLMDSISQQKSETIPSEITNTKKGKSHKSQSSMAHKGEIHDPPPEAVQTVTPVEVHIGTWFTTTPEGNRIGTKGLERIADLTPLLSFQATDPVNGTVMTTREDKVVIVERKDGTRIVDHADGTRITTFYQVYEDQIILPDDQETTEGPRTVTRQVKCMRVESSRYATVIANCEDSSCCATFGDGTTIIAKPQGTYQVLPPNTGSLYIDKDCSAVYCHESSSNIYYPFQKREQLRAGRYIMRHTSEVICEVLDPEGNTFQVMADGSISTILPEKKLEDDLNEKTEGYDSLSSMHLEKNHQQIYGEHVPRFFVMYADGSGMELLRDSDIEEYLSLAYKESNTVVLQEPVQEQPGTLTITVLRPFHEASPWQVKKEDTIVPPNLRSRSWETFPSVEKKTPGPPFGTQIWKGLCIESKQLVSAPGAILKSPSVLQMRQFIQHEVIKNEVKLRLQVSLKDYINYILKKEDELQEMMVKDSRTEEERGNAADLLKLVMSFPKMEETTKSHVTEVAAHLTDLFKQSLATPPKCPPDTFGKDFFEKTWRHTASSKRWKEKIDKTRKEIETTQNYLMDIKNRIIPPFFKSELNQLYQSQYNHLDSLSKKLPSFTKKNEDANETAVQDTSDLNLDFKPHKVSEQKSSSVPSLPKPEISADKKDFTAQNQTENLTKSPEEAESYEPVKIPTQSLLQDVAGQTRKEKVKLPHYLLSSKPKSQPLAKVQDSVGGKVNTSSVASAAINNAKSSLFGFHLLPSSVKFGVLKEGHTYATVVKLKNVGVDFCRFKVKQPPPSTGLKVTYKPGPVAAGMQTELNIELFATAVGEDGAKGSAHISHNIEIMTEHEVLFLPVEATVLTSSNYDKRPKDFPQGKENPMVQRTSTIYSSTLGVFMSRKVSPH

Target ORF information:

RefSeq Version	XM_006710426.3
Organism	Homo sapiens(human)
Definition	Homo sapiens sperm associated antigen 17 (SPAG17), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006710426.3

ORF Insert Sequence:

ATGGCACCCA AGAAGGAGAA AGGAGGAACT GTGAACACCA GTTCTAAGAT ATGGGAACCC 
TCGCTCATAG CTGCACAGTT CAATCAGAAC GATTGGCAGG CCTCCATTGC TTTTGTGGTT 
GGGAACCAGA TTGAAGATGA TCTTCTCATC CAAGCCCTTA CCGTGGCTGT CCAGGTCCCT 
CAGCGTAAAC TCTTCAGTAT GGTGTCGTGG CAAGACATTC TCCAGCAGAT TAATGAAATA 
AATACACTTG TTGGATCTGC TTCATCTAAA AAGGCAAAAA AACCTGTAGG TGGTAATGCT 
CCTTTATATT ATGAGGTGTT AACGGCAGCA AAAGCAATTA TGGATAGTGG AGAGAAATTA 
ACCTTACCAC TGATAGGGAA ACTCTTGAAA TTTCAACTTC TCCAGATTAA ATTTAAGGAC 
CAACAGCGAC GGGAAAATGA AAAGAAGGTA ATAGAAGACA AACCTAAGTT AGAAAAGGAT 
AAAGGGAAAG CAAAATCTCC CAAGGAGAAA AAGGCTCCAA GTGCCAAGCC TGCCAAAGGA 
AAGGGAAAGG ATCAGCCTGA GGCAAATGCA CCAGTGAAAA AGACCACCCA GTTAAAGCGG 
AGAGGAGAAG ACGACCACAC CAATCGTTAC ATTGACGATG AGCCAGATGA TGGTGCCCAA 
CATTACATTA TAGTTGTGGG CTTTAACAAT CCTCAGCTAT TAGCAATTAT GGCTGAGCTT 
GGCATTCCTA TAACCAGCGT GATTAAAATA TCTTCAGAGA ATTATGAACC TCTGCAGACA 
CACCTGGCAG CAGTTAACCA GCAGCAGGAA GTTCTTCTTC AGTCAGAAGA TCTAGAAGCA 
GAAAAATTGA AGAAAGAAAA TGCCATAAAA GAGCTTAAAA CTTTCTGGAA GTACTTGGAA 
CCAGTCCTGA ATAATGAGAA ACCTGAAACA AATCTCTTTG ATGTTGCTCG ACTTGAGTAC 
ATGGTCAAAG CAGCTGATTT TCCTTCTGAC TGGTCAGATG GTGAGATGAT GCTGAAATTG 
GGCACTGATA TTTTTGAAAA TATTGCCTGC TTGATGTATG ACATCCTGGA TTGGAAAAGG 
CAGCACCAGC ACTATTTGGA AAGCATGCAG CTTATTAATG TTCCACAAGT GGTTAATGAG 
AAACCTGTAT TAGAAGCCAT GCCAACTTCA GAGGCTCCAC AACCTGCTGT ACCAGCTCCT 
GGAAAGAAGA AAGCACAGTA TGAAGAACCG CAAGCTCCAC CACCAGTGAC TTCAGTCATC 
ACAACTGAAG TAGACATGAG ATATTACAAT TATTTGCTGA ATCCAATTCG AGAGGAATTC 
ATTTCTGTGC CCCTGATACT GCATTGTATG CTGGAACAGG TTGTTGCAAC TGAAGAAGAT 
CTCGTCCCAC CCAGTCTGCG GGAGCCATCC CCCAGAGCAG ACGGGCTAGA CCACAGAATC 
GCAGCTCACA TTGTGTCCCT TCTGCCCTCA CTCTGTCTCT CAGAGAGGGA GAAAAAGAAT 
CTTCATGACA TATTTTTATC TGAAGAAGAA AATGAAAGCA AAGCAGTGCC CAAAGGCCCC 
CTCCTACTGA ACTATCATGA TGCACACGCC CACAAGAAGT ACGCACTACA GGACCAAAAG 
AATTTTGATC CAGTTCAAAT TGAGCAGGAG ATGCAGTCCA AGTTGCCACT GTGGGAATTT 
CTTCAATTCC CTCTACCCCC ACCATGGAAC AACACTAAAC GTCTAGCTAC AATTCATGAG 
CTTATGCACT TTTGTACGAG TGATGTCTTG AGCTGGAATG AAGTAGAACG AGCCTTCAAG 
GTGTTTACTT TTGAGAGCCT GAAGCTCTCT GAGGTTGATG AAAAAGGGAA ACTGAAACCT 
TCTGGGATGA TGTGTGGGTC AGATTCTGAA ATGTTCAACA TACCGTGGGA CAACCCTGCC 
AGATTTGCTA AACAGATAAG GCAGCAATAT GTCATGAAAA TGAATACTCA AGAGGCCAAG 
CAGAAAGCAG ATATTAAAAT CAAAGACAGA ACACTATTTG TGGATCAGAA TTTGTCAATG 
TCTGTGCAAG ATAATGAAAG CAACCGAGAA CCTTCAGATC CTAGTCAGTG TGATGCTAAC 
AATATGAAGC ATTCTGACTT GAATAATCTC AAACTCTCAG TCCCTGATAA TAGACAGCTG 
TTAGAGCAGG AGAGCATCAT GAAGGCTCAG CCCCAACATG AGTCTCTGGA GCAGACCACA 
AACAATGAGA TCAAAGATGA TGCAGTCACA AAGGCTGATT CTCATGAAAA GAAACCCAAG 
AAGATGATGG TGGAAGCAGA TTTAGAGGAC ATAAAGAAAA CACAGCAGCG CAGTCTAATG 
GACTGGAGTT TTACTGAACA TTTTAAACCG AAAGTACTGC TTCAGGTCCT TCAAGAAGCC 
CATAAGCAAT ATAGGTGTGT TGATTCTTAC TACCACACCC AAGACAACTC TTTACTTTTA 
GTCTTTCACA ATCCAATGAA TAGACAACGT TTGCATTGTG AATATTGGAA CATTGCTCTC 
CACTCCAATG TTGGATTCAG GAATTATTTG GAACTTGTTG CAAAATCTAT TCAAGATTGG 
ATTACAAAAG AAGAAGCTAT ATATCAGGAA TCTAAAATGA ATGAGAAAAT CATCAGGACC 
AGAGCTGAGC TGGAATTGAA ATCTTCTGCT AATGCCAAAC TTACTTCTGC TAGCAAAATT 
TTTTCCATTA AAGAATCTAA AAGTAACAAA GGAATCAGCA AAACAGAGAT ATCAGATCAA 
GAAAAAGAAA AAGAGAAGGA AAAGATTCCT TTCATTTTAG AAGGCTCTCT CAAGGCATGG 
AAAGAAGAGC AACATCGATT AGCAGAAGAG GAGCGCTTAA GGGAAGAAAA GAAAGCAGAG 
AAGAAGGGTA AAGAAGCTGG TAAAAAGAAA GGCAAGGATA ACGCAGAGAA AGAGGATAGT 
AGGTCTTTGA AGAAAAAATC ACCTTACAAG GAGAAATCTA AAGAAGAACA AGTCAAGATC 
CAAGAAGTAA CAGAAGAGTC CCCCCACCAA CCAGAACCTA AGATAACTTA CCCGTTTCAC 
GGATACAATA TGGGAAATAT ACCCACTCAA ATCTCAGGGT CAAATTACTA CCTGTATCCT 
TCTGATGGGG GGCAGATTGA AGTGGAAAAG ACAATGTTTG AAAAAGGCCC AACTTTTATC 
AAAGTGAGAG TGGTAAAGGA CAACCACAAT TTTATGATTC ATTTAAATGA CCCTAAGGAA 
ATTGTGAAAA AGGAAGAGAA AGGGGATTAT TATTTAGAAG AGGAAGAAGA AGGAGATGAG 
GAACAAAGTC TTGAAACGGA AGATAAGGAT CCTGATTTAG AAACAATATT GAATATCCCT 
TCAGCACTCA CTCCAACAGT GGTTCCTGTT ATAGTGACCG TTCCTCAAAG CAAAGCTAAA 
GGGAAAATAA AAGGCAAAGA AAAACCCAAA GAATCCCTTA AAGAAGAAGA ACACCCAAAA 
GAAGAAGAGA AAAAGGAAGA AGAAGTAGAA CCAGAACCTG TTTTACAAGA GACTTTGGAT 
GTTCCCACCT TCCAGAGCCT AAATGTGTCT TGCCCCAGTG GGCTCCTGTT GACTTTCATT 
GGACAAGAAT CTACAGGTCA ATATGTTATA GATGAGGAAC CCACCTGGGA CATCATGGTC 
CGTCAGAGCT ACCCCCAGAG GGTGAAGCAC TATGAGTTCT ATAAAACGGT GATGCCACCC 
GCAGAGCAGG AGGCTTCAAG GGTTATCACC AGTCAAGGCA CTGTTGTCAA ATATATGTTG 
GATGGATCCA CACAGATTCT CTTTGCAGAT GGTGCTGTGA GCAGGAGTCC CAATTCAGGT 
CTTATTTGTC CTCCTTCTGA AATGCCAGCA ACGCCTCACA GTGGAGATTT GATGGACTCT 
ATTTCTCAGC AGAAATCAGA AACGATACCA TCTGAGATTA CCAACACAAA GAAAGGAAAA 
AGTCACAAAA GTCAGTCATC AATGGCCCAT AAGGGTGAAA TCCATGACCC TCCTCCAGAG 
GCAGTTCAAA CTGTAACTCC TGTGGAGGTT CACATAGGCA CCTGGTTTAC AACCACACCT 
GAAGGAAATC GGATCGGCAC CAAAGGATTA GAAAGAATAG CAGACTTGAC CCCATTGTTA 
TCCTTTCAGG CCACAGATCC TGTCAATGGA ACGGTTATGA CAACTCGAGA AGACAAAGTT 
GTCATAGTTG AAAGGAAAGA TGGTACTCGG ATAGTGGATC ATGCTGATGG TACCAGAATC 
ACAACCTTTT ATCAAGTTTA TGAAGATCAA ATTATTCTGC CAGATGATCA AGAAACAACC 
GAGGGTCCTC GGACTGTCAC CAGGCAGGTG AAGTGTATGC GGGTAGAAAG CTCACGCTAT 
GCCACTGTTA TCGCCAACTG TGAGGACAGT AGCTGCTGTG CCACCTTTGG AGATGGAACA 
ACTATTATTG CAAAGCCACA GGGAACATAC CAGGTGTTAC CTCCAAACAC AGGCTCTCTT 
TATATTGACA AGGATTGTTC AGCTGTGTAC TGCCATGAGT CAAGCAGTAA TATATACTAT 
CCTTTTCAAA AGCGTGAGCA GCTGCGAGCT GGCAGGTACA TCATGAGGCA TACTTCAGAG 
GTTATCTGTG AGGTTCTGGA TCCTGAGGGA AACACTTTTC AGGTCATGGC TGATGGTAGC 
ATATCAACTA TATTACCTGA AAAAAAATTG GAAGATGATT TAAATGAGAA AACTGAGGGC 
TATGATAGTC TGTCCTCTAT GCACCTTGAA AAGAATCATC AGCAAATCTA TGGTGAACAT 
GTCCCCAGGT TTTTTGTTAT GTATGCTGAT GGATCAGGAA TGGAACTTCT TCGAGACAGT 
GACATAGAAG AATATCTATC TTTGGCATAT AAAGAATCAA ATACTGTTGT TCTCCAAGAG 
CCAGTGCAGG AACAGCCAGG CACCCTAACC ATCACAGTCC TTCGCCCTTT CCATGAAGCA 
TCACCATGGC AAGTAAAAAA GGAAGATACA ATTGTCCCTC CTAATCTCCG GTCAAGGTCA 
TGGGAAACAT TTCCCTCAGT TGAGAAAAAA ACTCCAGGAC CTCCGTTTGG TACTCAGATT 
TGGAAAGGCC TTTGCATTGA GTCCAAACAG CTAGTGAGTG CCCCGGGTGC CATACTCAAG 
AGCCCCAGTG TGCTACAGAT GCGCCAATTC ATTCAGCATG AGGTCATAAA GAATGAGGTG 
AAACTGAGGC TGCAGGTTTC CCTTAAGGAT TACATAAACT ATATTCTAAA GAAAGAAGAT 
GAGCTGCAGG AAATGATGGT TAAAGATTCC AGAACTGAGG AGGAGAGAGG CAATGCTGCT 
GATCTCCTCA AGCTGGTTAT GTCTTTCCCT AAAATGGAGG AAACTACAAA AAGTCATGTT 
ACTGAAGTTG CAGCTCACCT AACTGATTTA TTCAAGCAGT CTTTGGCTAC GCCTCCAAAA 
TGCCCACCAG ACACATTTGG TAAAGATTTC TTTGAAAAGA CATGGAGACA CACAGCATCC 
TCAAAACGCT GGAAAGAAAA GATAGACAAA ACGAGGAAGG AAATTGAGAC AACACAGAAT 
TACCTAATGG ATATTAAGAA CCGCATAATA CCACCCTTTT TTAAATCTGA ATTGAACCAG 
TTATATCAGT CTCAGTATAA TCACCTGGAC AGTCTTTCCA AAAAACTGCC TTCTTTTACA 
AAGAAAAATG AAGATGCAAA CGAAACAGCT GTTCAAGATA CATCTGATCT TAATCTAGAT 
TTCAAGCCAC ATAAGGTTTC AGAACAGAAA TCCTCAAGTG TGCCTAGTCT TCCAAAACCA 
GAGATTTCTG CAGATAAGAA GGATTTCACT GCTCAGAACC AAACTGAAAA TTTAACAAAA 
TCTCCTGAAG AAGCAGAATC TTATGAGCCC GTGAAAATTC CAACCCAGTC CTTGCTGCAG 
GATGTTGCGG GACAAACAAG AAAAGAAAAA GTGAAGTTGC CTCATTATTT GCTGAGTTCC 
AAGCCTAAGT CTCAACCTCT TGCAAAGGTG CAAGATTCTG TTGGAGGAAA AGTGAACACA 
TCCTCTGTTG CATCTGCTGC CATTAATAAT GCAAAGTCAT CCCTTTTTGG GTTCCATCTT 
CTCCCATCAT CAGTCAAGTT TGGAGTGCTT AAGGAAGGAC ATACCTATGC CACAGTTGTA 
AAGCTCAAGA ATGTTGGAGT GGACTTCTGC AGGTTTAAAG TAAAGCAGCC CCCACCCAGC 
ACAGGACTGA AAGTGACTTA CAAACCTGGA CCTGTGGCAG CTGGTATGCA GACAGAACTG 
AATATAGAGT TATTTGCCAC AGCTGTTGGA GAGGATGGGG CCAAGGGATC AGCACACATC 
TCTCACAATA TCGAGATTAT GACAGAGCAT GAGGTTCTGT TCCTACCTGT GGAAGCAACT 
GTTTTAACAA GCAGCAATTA TGATAAACGA CCAAAAGACT TTCCCCAGGG AAAAGAAAAT 
CCAATGGTCC AGAGAACTTC TACAATTTAT TCCTCCACAC TTGGAGTCTT CATGTCTCGT 
AAAGTTTCTC CACATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPAG17 ( XM_006710427.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_006710490.1 Homo sapiens sperm associated antigen 17 (SPAG17), transcript variant X4, mRNA.

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CloneID	OHu33132
Clone ID Related Accession (Same CDS sequence)	XM_006710427.3
Accession Version	XM_006710427.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6477bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	sperm-associated antigen 17 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006710427.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCACCCA AGAAGGAGAA AGGAGGAACT GTGAACACCA GTTCTAAGAT ATGGGAACCC 
TCGCTCATAG CTGCACAGTT CAATCAGAAC GATTGGCAGG CCTCCATTGC TTTTGTGGTT 
GGGAACCAGA TTGAAGATGA TCTTCTCATC CAAGCCCTTA CCGTGGCTGT CCAGGTCCCT 
CAGCGTAAAC TCTTCAGTAT GGTGTCGTGG CAAGACATTC TCCAGCAGAT TAATGAAATA 
AATACACTTG TTGGATCTGC TTCATCTAAA AAGGCAAAAA AACCTGTAGG TGGTAATGCT 
CCTTTATATT ATGAGGTGTT AACGGCAGCA AAAGCAATTA TGGATAGTGG AGAGAAATTA 
ACCTTACCAC TGATAGGGAA ACTCTTGAAA TTTCAACTTC TCCAGATTAA ATTTAAGGAC 
CAACAGCGAC GGGAAAATGA AAAGAAGGTA ATAGAAGACA AACCTAAGTT AGAAAAGGAT 
AAAGGGAAAG CAAAATCTCC CAAGGAGAAA AAGGCTCCAA GTGCCAAGCC TGCCAAAGGA 
AAGGGAAAGG ATCAGCCTGA GGCAAATGCA CCAGTGAAAA AGACCACCCA GTTAAAGCGG 
AGAGGAGAAG ACGACCACAC CAATCGTTAC ATTGATCTAG AAGCAGAAAA ATTGAAGAAA 
GAAAATGCCA TAAAAGAGCT TAAAACTTTC TGGAAGTACT TGGAACCAGT CCTGAATAAT 
GAGAAACCTG AAACAAATCT CTTTGATGTT GCTCGACTTG AGTACATGGT CAAAGCAGCT 
GATTTTCCTT CTGACTGGTC AGATGGTGAG ATGATGCTGA AATTGGGCAC TGATATTTTT 
GAAAATATTG CCTGCTTGAT GTATGACATC CTGGATTGGA AAAGGCAGCA CCAGCACTAT 
TTGGAAAGCA TGCAGCTTAT TAATGTTCCA CAAGTGGTTA ATGAGAAACC TGTATTAGAA 
GCCATGCCAA CTTCAGAGGC TCCACAACCT GCTGTACCAG CTCCTGGAAA GAAGAAAGCA 
CAGTATGAAG AACCGCAAGC TCCACCACCA GTGACTTCAG TCATCACAAC TGAAGTAGAC 
ATGAGATATT ACAATTATTT GCTGAATCCA ATTCGAGAGG AATTCATTTC TGTGCCCCTG 
ATACTGCATT GTATGCTGGA ACAGGTTGTT GCAACTGAAG AAGATCTCGT CCCACCCAGT 
CTGCGGGAGC CATCCCCCAG AGCAGACGGG CTAGACCACA GAATCGCAGC TCACATTGTG 
TCCCTTCTGC CCTCACTCTG TCTCTCAGAG AGGGAGAAAA AGAATCTTCA TGACATATTT 
TTATCTGAAG AAGAAAATGA AAGCAAAGCA GTGCCCAAAG GCCCCCTCCT ACTGAACTAT 
CATGATGCAC ACGCCCACAA GAAGTACGCA CTACAGGACC AAAAGAATTT TGATCCAGTT 
CAAATTGAGC AGGAGATGCA GTCCAAGTTG CCACTGTGGG AATTTCTTCA ATTCCCTCTA 
CCCCCACCAT GGAACAACAC TAAACGTCTA GCTACAATTC ATGAGCTTAT GCACTTTTGT 
ACGAGTGATG TCTTGAGCTG GAATGAAGTA GAACGAGCCT TCAAGGTGTT TACTTTTGAG 
AGCCTGAAGC TCTCTGAGGT TGATGAAAAA GGGAAACTGA AACCTTCTGG GATGATGTGT 
GGGTCAGATT CTGAAATGTT CAACATACCG TGGGACAACC CTGCCAGATT TGCTAAACAG 
ATAAGGCAGC AATATGTCAT GAAAATGAAT ACTCAAGAGG CCAAGCAGAA AGCAGATATT 
AAAATCAAAG ACAGAACACT ATTTGTGGAT CAGAATTTGT CAATGTCTGT GCAAGATAAT 
GAAAGCAACC GAGAACCTTC AGATCCTAGT CAGTGTGATG CTAACAATAT GAAGCATTCT 
GACTTGAATA ATCTCAAACT CTCAGTCCCT GATAATAGAC AGCTGTTAGA GCAGGAGAGC 
ATCATGAAGG CTCAGCCCCA ACATGAGTCT CTGGAGCAGA CCACAAACAA TGAGATCAAA 
GATGATGCAG TCACAAAGGC TGATTCTCAT GAAAAGAAAC CCAAGAAGAT GATGGTGGAA 
GCAGATTTAG AGGACATAAA GAAAACACAG CAGCGCAGTC TAATGGACTG GAGTTTTACT 
GAACATTTTA AACCGAAAGT ACTGCTTCAG GTCCTTCAAG AAGCCCATAA GCAATATAGG 
TGTGTTGATT CTTACTACCA CACCCAAGAC AACTCTTTAC TTTTAGTCTT TCACAATCCA 
ATGAATAGAC AACGTTTGCA TTGTGAATAT TGGAACATTG CTCTCCACTC CAATGTTGGA 
TTCAGGAATT ATTTGGAACT TGTTGCAAAA TCTATTCAAG ATTGGATTAC AAAAGAAGAA 
GCTATATATC AGGAATCTAA AATGAATGAG AAAATCATCA GGACCAGAGC TGAGCTGGAA 
TTGAAATCTT CTGCTAATGC CAAACTTACT TCTGCTAGCA AAATTTTTTC CATTAAAGAA 
TCTAAAAGTA ACAAAGGAAT CAGCAAAACA GAGATATCAG ATCAAGAAAA AGAAAAAGAG 
AAGGAAAAGA TTCCTTTCAT TTTAGAAGGC TCTCTCAAGG CATGGAAAGA AGAGCAACAT 
CGATTAGCAG AAGAGGAGCG CTTAAGGGAA GAAAAGAAAG CAGAGAAGAA GGGTAAAGAA 
GCTGGTAAAA AGAAAGGCAA GGATAACGCA GAGAAAGAGG ATAGTAGGTC TTTGAAGAAA 
AAATCACCTT ACAAGGAGAA ATCTAAAGAA GAACAAGTCA AGATCCAAGA AGTAACAGAA 
GAGTCCCCCC ACCAACCAGA ACCTAAGATA ACTTACCCGT TTCACGGATA CAATATGGGA 
AATATACCCA CTCAAATCTC AGGGTCAAAT TACTACCTGT ATCCTTCTGA TGGGGGGCAG 
ATTGAAGTGG AAAAGACAAT GTTTGAAAAA GGCCCAACTT TTATCAAAGT GAGAGTGGTA 
AAGGACAACC ACAATTTTAT GATTCATTTA AATGACCCTA AGGAAATTGT GAAAAAGGAA 
GAGAAAGGGG ATTATTATTT AGAAGAGGAA GAAGAAGGAG ATGAGGAACA AAGTCTTGAA 
ACGGAAGTAT CAGATGCAAA GAATAAAGCT TTCAGCAAGT TTGGATCTTT TTCTGCCACC 
TTAGAAAATG GAATCTGCCT CTCGATAAGT TACTATGGAT CAAATGGAAT GGCACCAGAA 
GATAAGGATC CTGATTTAGA AACAATATTG AATATCCCTT CAGCACTCAC TCCAACAGTG 
GTTCCTGTTA TAGTGACCGT TCCTCAAAGC AAAGCTAAAG GGAAAATAAA AGGCAAAGAA 
AAACCCAAAG AATCCCTTAA AGAAGAAGAA CACCCAAAAG AAGAAGAGAA AAAGGAAGAA 
GAAGTAGAAC CAGAACCTGT TTTACAAGAG ACTTTGGATG TTCCCACCTT CCAGAGCCTA 
AATGTGTCTT GCCCCAGTGG GCTCCTGTTG ACTTTCATTG GACAAGAATC TACAGGTCAA 
TATGTTATAG ATGAGGAACC CACCTGGGAC ATCATGGTCC GTCAGAGCTA CCCCCAGAGG 
GTGAAGCACT ATGAGTTCTA TAAAACGGTG ATGCCACCCG CAGAGCAGGA GGCTTCAAGG 
GTTATCACCA GTCAAGGCAC TGTTGTCAAA TATATGTTGG ATGGATCCAC ACAGATTCTC 
TTTGCAGATG GTGCTGTGAG CAGGAGTCCC AATTCAGGTC TTATTTGTCC TCCTTCTGAA 
ATGCCAGCAA CGCCTCACAG TGGAGATTTG ATGGACTCTA TTTCTCAGCA GAAATCAGAA 
ACGATACCAT CTGAGATTAC CAACACAAAG AAAGGAAAAA GTCACAAAAG TCAGTCATCA 
ATGGCCCATA AGGGTGAAAT CCATGACCCT CCTCCAGAGG CAGTTCAAAC TGTAACTCCT 
GTGGAGGTTC ACATAGGCAC CTGGTTTACA ACCACACCTG AAGGAAATCG GATCGGCACC 
AAAGGATTAG AAAGAATAGC AGACTTGACC CCATTGTTAT CCTTTCAGGC CACAGATCCT 
GTCAATGGAA CGGTTATGAC AACTCGAGAA GACAAAGTTG TCATAGTTGA AAGGAAAGAT 
GGTACTCGGA TAGTGGATCA TGCTGATGGT ACCAGAATCA CAACCTTTTA TCAAGTTTAT 
GAAGATCAAA TTATTCTGCC AGATGATCAA GAAACAACCG AGGGTCCTCG GACTGTCACC 
AGGCAGGTGA AGTGTATGCG GGTAGAAAGC TCACGCTATG CCACTGTTAT CGCCAACTGT 
GAGGACAGTA GCTGCTGTGC CACCTTTGGA GATGGAACAA CTATTATTGC AAAGCCACAG 
GGAACATACC AGGTGTTACC TCCAAACACA GGCTCTCTTT ATATTGACAA GGATTGTTCA 
GCTGTGTACT GCCATGAGTC AAGCAGTAAT ATATACTATC CTTTTCAAAA GCGTGAGCAG 
CTGCGAGCTG GCAGGTACAT CATGAGGCAT ACTTCAGAGG TTATCTGTGA GGTTCTGGAT 
CCTGAGGGAA ACACTTTTCA GGTCATGGCT GATGGTAGCA TATCAACTAT ATTACCTGAA 
AAAAAATTGG AAGATGATTT AAATGAGAAA ACTGAGGGCT ATGATAGTCT GTCCTCTATG 
CACCTTGAAA AGAATCATCA GCAAATCTAT GGTGAACATG TCCCCAGGTT TTTTGTTATG 
TATGCTGATG GATCAGGAAT GGAACTTCTT CGAGACAGTG ACATAGAAGA ATATCTATCT 
TTGGCATATA AAGAATCAAA TACTGTTGTT CTCCAAGAGC CAGTGCAGGA ACAGCCAGGC 
ACCCTAACCA TCACAGTCCT TCGCCCTTTC CATGAAGCAT CACCATGGCA AGTAAAAAAG 
GAAGATACAA TTGTCCCTCC TAATCTCCGG TCAAGGTCAT GGGAAACATT TCCCTCAGTT 
GAGAAAAAAA CTCCAGGACC TCCGTTTGGT ACTCAGATTT GGAAAGGCCT TTGCATTGAG 
TCCAAACAGC TAGTGAGTGC CCCGGGTGCC ATACTCAAGA GCCCCAGTGT GCTACAGATG 
CGCCAATTCA TTCAGCATGA GGTCATAAAG AATGAGGTGA AACTGAGGCT GCAGGTTTCC 
CTTAAGGATT ACATAAACTA TATTCTAAAG AAAGAAGATG AGCTGCAGGA AATGATGGTT 
AAAGATTCCA GAACTGAGGA GGAGAGAGGC AATGCTGCTG ATCTCCTCAA GCTGGTTATG 
TCTTTCCCTA AAATGGAGGA AACTACAAAA AGTCATGTTA CTGAAGTTGC AGCTCACCTA 
ACTGATTTAT TCAAGCAGTC TTTGGCTACG CCTCCAAAAT GCCCACCAGA CACATTTGGT 
AAAGATTTCT TTGAAAAGAC ATGGAGACAC ACAGCATCCT CAAAACGCTG GAAAGAAAAG 
ATAGACAAAA CGAGGAAGGA AATTGAGACA ACACAGAATT ACCTAATGGA TATTAAGAAC 
CGCATAATAC CACCCTTTTT TAAATCTGAA TTGAACCAGT TATATCAGTC TCAGTATAAT 
CACCTGGACA GTCTTTCCAA AAAACTGCCT TCTTTTACAA AGAAAAATGA AGATGCAAAC 
GAAACAGCTG TTCAAGATAC ATCTGATCTT AATCTAGATT TCAAGCCACA TAAGGTTTCA 
GAACAGAAAT CCTCAAGTGT GCCTAGTCTT CCAAAACCAG AGATTTCTGC AGATAAGAAG 
GATTTCACTG CTCAGAACCA AACTGAAAAT TTAACAAAAT CTCCTGAAGA AGCAGAATCT 
TATGAGCCCG TGAAAATTCC AACCCAGTCC TTGCTGCAGG ATGTTGCGGG ACAAACAAGA 
AAAGAAAAAG TGAAGTTGCC TCATTATTTG CTGAGTTCCA AGCCTAAGTC TCAACCTCTT 
GCAAAGGTGC AAGATTCTGT TGGAGGAAAA GTGAACACAT CCTCTGTTGC ATCTGCTGCC 
ATTAATAATG CAAAGTCATC CCTTTTTGGG TTCCATCTTC TCCCATCATC AGTCAAGTTT 
GGAGTGCTTA AGGAAGGACA TACCTATGCC ACAGTTGTAA AGCTCAAGAA TGTTGGAGTG 
GACTTCTGCA GGTTTAAAGT AAAGCAGCCC CCACCCAGCA CAGGACTGAA AGTGACTTAC 
AAACCTGGAC CTGTGGCAGC TGGTATGCAG ACAGAACTGA ATATAGAGTT ATTTGCCACA 
GCTGTTGGAG AGGATGGGGC CAAGGGATCA GCACACATCT CTCACAATAT CGAGATTATG 
ACAGAGCATG AGGTTCTGTT CCTACCTGTG GAAGCAACTG TTTTAACAAG CAGCAATTAT 
GATAAACGAC CAAAAGACTT TCCCCAGGGA AAAGAAAATC CAATGGTCCA GAGAACTTCT 
ACAATTTATT CCTCCACACT TGGAGTCTTC ATGTCTCGTA AAGTTTCTCC ACATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006710490.1
CDS	69..6545
Translation	MAPKKEKGGTVNTSSKIWEPSLIAAQFNQNDWQASIAFVVGNQIEDDLLIQALTVAVQVPQRKLFSMVSWQDILQQINEINTLVGSASSKKAKKPVGGNAPLYYEVLTAAKAIMDSGEKLTLPLIGKLLKFQLLQIKFKDQQRRENEKKVIEDKPKLEKDKGKAKSPKEKKAPSAKPAKGKGKDQPEANAPVKKTTQLKRRGEDDHTNRYIDLEAEKLKKENAIKELKTFWKYLEPVLNNEKPETNLFDVARLEYMVKAADFPSDWSDGEMMLKLGTDIFENIACLMYDILDWKRQHQHYLESMQLINVPQVVNEKPVLEAMPTSEAPQPAVPAPGKKKAQYEEPQAPPPVTSVITTEVDMRYYNYLLNPIREEFISVPLILHCMLEQVVATEEDLVPPSLREPSPRADGLDHRIAAHIVSLLPSLCLSEREKKNLHDIFLSEEENESKAVPKGPLLLNYHDAHAHKKYALQDQKNFDPVQIEQEMQSKLPLWEFLQFPLPPPWNNTKRLATIHELMHFCTSDVLSWNEVERAFKVFTFESLKLSEVDEKGKLKPSGMMCGSDSEMFNIPWDNPARFAKQIRQQYVMKMNTQEAKQKADIKIKDRTLFVDQNLSMSVQDNESNREPSDPSQCDANNMKHSDLNNLKLSVPDNRQLLEQESIMKAQPQHESLEQTTNNEIKDDAVTKADSHEKKPKKMMVEADLEDIKKTQQRSLMDWSFTEHFKPKVLLQVLQEAHKQYRCVDSYYHTQDNSLLLVFHNPMNRQRLHCEYWNIALHSNVGFRNYLELVAKSIQDWITKEEAIYQESKMNEKIIRTRAELELKSSANAKLTSASKIFSIKESKSNKGISKTEISDQEKEKEKEKIPFILEGSLKAWKEEQHRLAEEERLREEKKAEKKGKEAGKKKGKDNAEKEDSRSLKKKSPYKEKSKEEQVKIQEVTEESPHQPEPKITYPFHGYNMGNIPTQISGSNYYLYPSDGGQIEVEKTMFEKGPTFIKVRVVKDNHNFMIHLNDPKEIVKKEEKGDYYLEEEEEGDEEQSLETEVSDAKNKAFSKFGSFSATLENGICLSISYYGSNGMAPEDKDPDLETILNIPSALTPTVVPVIVTVPQSKAKGKIKGKEKPKESLKEEEHPKEEEKKEEEVEPEPVLQETLDVPTFQSLNVSCPSGLLLTFIGQESTGQYVIDEEPTWDIMVRQSYPQRVKHYEFYKTVMPPAEQEASRVITSQGTVVKYMLDGSTQILFADGAVSRSPNSGLICPPSEMPATPHSGDLMDSISQQKSETIPSEITNTKKGKSHKSQSSMAHKGEIHDPPPEAVQTVTPVEVHIGTWFTTTPEGNRIGTKGLERIADLTPLLSFQATDPVNGTVMTTREDKVVIVERKDGTRIVDHADGTRITTFYQVYEDQIILPDDQETTEGPRTVTRQVKCMRVESSRYATVIANCEDSSCCATFGDGTTIIAKPQGTYQVLPPNTGSLYIDKDCSAVYCHESSSNIYYPFQKREQLRAGRYIMRHTSEVICEVLDPEGNTFQVMADGSISTILPEKKLEDDLNEKTEGYDSLSSMHLEKNHQQIYGEHVPRFFVMYADGSGMELLRDSDIEEYLSLAYKESNTVVLQEPVQEQPGTLTITVLRPFHEASPWQVKKEDTIVPPNLRSRSWETFPSVEKKTPGPPFGTQIWKGLCIESKQLVSAPGAILKSPSVLQMRQFIQHEVIKNEVKLRLQVSLKDYINYILKKEDELQEMMVKDSRTEEERGNAADLLKLVMSFPKMEETTKSHVTEVAAHLTDLFKQSLATPPKCPPDTFGKDFFEKTWRHTASSKRWKEKIDKTRKEIETTQNYLMDIKNRIIPPFFKSELNQLYQSQYNHLDSLSKKLPSFTKKNEDANETAVQDTSDLNLDFKPHKVSEQKSSSVPSLPKPEISADKKDFTAQNQTENLTKSPEEAESYEPVKIPTQSLLQDVAGQTRKEKVKLPHYLLSSKPKSQPLAKVQDSVGGKVNTSSVASAAINNAKSSLFGFHLLPSSVKFGVLKEGHTYATVVKLKNVGVDFCRFKVKQPPPSTGLKVTYKPGPVAAGMQTELNIELFATAVGEDGAKGSAHISHNIEIMTEHEVLFLPVEATVLTSSNYDKRPKDFPQGKENPMVQRTSTIYSSTLGVFMSRKVSPH

Target ORF information:

RefSeq Version	XM_006710427.3
Organism	Homo sapiens(human)
Definition	Homo sapiens sperm associated antigen 17 (SPAG17), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006710427.3

ORF Insert Sequence:

ATGGCACCCA AGAAGGAGAA AGGAGGAACT GTGAACACCA GTTCTAAGAT ATGGGAACCC 
TCGCTCATAG CTGCACAGTT CAATCAGAAC GATTGGCAGG CCTCCATTGC TTTTGTGGTT 
GGGAACCAGA TTGAAGATGA TCTTCTCATC CAAGCCCTTA CCGTGGCTGT CCAGGTCCCT 
CAGCGTAAAC TCTTCAGTAT GGTGTCGTGG CAAGACATTC TCCAGCAGAT TAATGAAATA 
AATACACTTG TTGGATCTGC TTCATCTAAA AAGGCAAAAA AACCTGTAGG TGGTAATGCT 
CCTTTATATT ATGAGGTGTT AACGGCAGCA AAAGCAATTA TGGATAGTGG AGAGAAATTA 
ACCTTACCAC TGATAGGGAA ACTCTTGAAA TTTCAACTTC TCCAGATTAA ATTTAAGGAC 
CAACAGCGAC GGGAAAATGA AAAGAAGGTA ATAGAAGACA AACCTAAGTT AGAAAAGGAT 
AAAGGGAAAG CAAAATCTCC CAAGGAGAAA AAGGCTCCAA GTGCCAAGCC TGCCAAAGGA 
AAGGGAAAGG ATCAGCCTGA GGCAAATGCA CCAGTGAAAA AGACCACCCA GTTAAAGCGG 
AGAGGAGAAG ACGACCACAC CAATCGTTAC ATTGATCTAG AAGCAGAAAA ATTGAAGAAA 
GAAAATGCCA TAAAAGAGCT TAAAACTTTC TGGAAGTACT TGGAACCAGT CCTGAATAAT 
GAGAAACCTG AAACAAATCT CTTTGATGTT GCTCGACTTG AGTACATGGT CAAAGCAGCT 
GATTTTCCTT CTGACTGGTC AGATGGTGAG ATGATGCTGA AATTGGGCAC TGATATTTTT 
GAAAATATTG CCTGCTTGAT GTATGACATC CTGGATTGGA AAAGGCAGCA CCAGCACTAT 
TTGGAAAGCA TGCAGCTTAT TAATGTTCCA CAAGTGGTTA ATGAGAAACC TGTATTAGAA 
GCCATGCCAA CTTCAGAGGC TCCACAACCT GCTGTACCAG CTCCTGGAAA GAAGAAAGCA 
CAGTATGAAG AACCGCAAGC TCCACCACCA GTGACTTCAG TCATCACAAC TGAAGTAGAC 
ATGAGATATT ACAATTATTT GCTGAATCCA ATTCGAGAGG AATTCATTTC TGTGCCCCTG 
ATACTGCATT GTATGCTGGA ACAGGTTGTT GCAACTGAAG AAGATCTCGT CCCACCCAGT 
CTGCGGGAGC CATCCCCCAG AGCAGACGGG CTAGACCACA GAATCGCAGC TCACATTGTG 
TCCCTTCTGC CCTCACTCTG TCTCTCAGAG AGGGAGAAAA AGAATCTTCA TGACATATTT 
TTATCTGAAG AAGAAAATGA AAGCAAAGCA GTGCCCAAAG GCCCCCTCCT ACTGAACTAT 
CATGATGCAC ACGCCCACAA GAAGTACGCA CTACAGGACC AAAAGAATTT TGATCCAGTT 
CAAATTGAGC AGGAGATGCA GTCCAAGTTG CCACTGTGGG AATTTCTTCA ATTCCCTCTA 
CCCCCACCAT GGAACAACAC TAAACGTCTA GCTACAATTC ATGAGCTTAT GCACTTTTGT 
ACGAGTGATG TCTTGAGCTG GAATGAAGTA GAACGAGCCT TCAAGGTGTT TACTTTTGAG 
AGCCTGAAGC TCTCTGAGGT TGATGAAAAA GGGAAACTGA AACCTTCTGG GATGATGTGT 
GGGTCAGATT CTGAAATGTT CAACATACCG TGGGACAACC CTGCCAGATT TGCTAAACAG 
ATAAGGCAGC AATATGTCAT GAAAATGAAT ACTCAAGAGG CCAAGCAGAA AGCAGATATT 
AAAATCAAAG ACAGAACACT ATTTGTGGAT CAGAATTTGT CAATGTCTGT GCAAGATAAT 
GAAAGCAACC GAGAACCTTC AGATCCTAGT CAGTGTGATG CTAACAATAT GAAGCATTCT 
GACTTGAATA ATCTCAAACT CTCAGTCCCT GATAATAGAC AGCTGTTAGA GCAGGAGAGC 
ATCATGAAGG CTCAGCCCCA ACATGAGTCT CTGGAGCAGA CCACAAACAA TGAGATCAAA 
GATGATGCAG TCACAAAGGC TGATTCTCAT GAAAAGAAAC CCAAGAAGAT GATGGTGGAA 
GCAGATTTAG AGGACATAAA GAAAACACAG CAGCGCAGTC TAATGGACTG GAGTTTTACT 
GAACATTTTA AACCGAAAGT ACTGCTTCAG GTCCTTCAAG AAGCCCATAA GCAATATAGG 
TGTGTTGATT CTTACTACCA CACCCAAGAC AACTCTTTAC TTTTAGTCTT TCACAATCCA 
ATGAATAGAC AACGTTTGCA TTGTGAATAT TGGAACATTG CTCTCCACTC CAATGTTGGA 
TTCAGGAATT ATTTGGAACT TGTTGCAAAA TCTATTCAAG ATTGGATTAC AAAAGAAGAA 
GCTATATATC AGGAATCTAA AATGAATGAG AAAATCATCA GGACCAGAGC TGAGCTGGAA 
TTGAAATCTT CTGCTAATGC CAAACTTACT TCTGCTAGCA AAATTTTTTC CATTAAAGAA 
TCTAAAAGTA ACAAAGGAAT CAGCAAAACA GAGATATCAG ATCAAGAAAA AGAAAAAGAG 
AAGGAAAAGA TTCCTTTCAT TTTAGAAGGC TCTCTCAAGG CATGGAAAGA AGAGCAACAT 
CGATTAGCAG AAGAGGAGCG CTTAAGGGAA GAAAAGAAAG CAGAGAAGAA GGGTAAAGAA 
GCTGGTAAAA AGAAAGGCAA GGATAACGCA GAGAAAGAGG ATAGTAGGTC TTTGAAGAAA 
AAATCACCTT ACAAGGAGAA ATCTAAAGAA GAACAAGTCA AGATCCAAGA AGTAACAGAA 
GAGTCCCCCC ACCAACCAGA ACCTAAGATA ACTTACCCGT TTCACGGATA CAATATGGGA 
AATATACCCA CTCAAATCTC AGGGTCAAAT TACTACCTGT ATCCTTCTGA TGGGGGGCAG 
ATTGAAGTGG AAAAGACAAT GTTTGAAAAA GGCCCAACTT TTATCAAAGT GAGAGTGGTA 
AAGGACAACC ACAATTTTAT GATTCATTTA AATGACCCTA AGGAAATTGT GAAAAAGGAA 
GAGAAAGGGG ATTATTATTT AGAAGAGGAA GAAGAAGGAG ATGAGGAACA AAGTCTTGAA 
ACGGAAGTAT CAGATGCAAA GAATAAAGCT TTCAGCAAGT TTGGATCTTT TTCTGCCACC 
TTAGAAAATG GAATCTGCCT CTCGATAAGT TACTATGGAT CAAATGGAAT GGCACCAGAA 
GATAAGGATC CTGATTTAGA AACAATATTG AATATCCCTT CAGCACTCAC TCCAACAGTG 
GTTCCTGTTA TAGTGACCGT TCCTCAAAGC AAAGCTAAAG GGAAAATAAA AGGCAAAGAA 
AAACCCAAAG AATCCCTTAA AGAAGAAGAA CACCCAAAAG AAGAAGAGAA AAAGGAAGAA 
GAAGTAGAAC CAGAACCTGT TTTACAAGAG ACTTTGGATG TTCCCACCTT CCAGAGCCTA 
AATGTGTCTT GCCCCAGTGG GCTCCTGTTG ACTTTCATTG GACAAGAATC TACAGGTCAA 
TATGTTATAG ATGAGGAACC CACCTGGGAC ATCATGGTCC GTCAGAGCTA CCCCCAGAGG 
GTGAAGCACT ATGAGTTCTA TAAAACGGTG ATGCCACCCG CAGAGCAGGA GGCTTCAAGG 
GTTATCACCA GTCAAGGCAC TGTTGTCAAA TATATGTTGG ATGGATCCAC ACAGATTCTC 
TTTGCAGATG GTGCTGTGAG CAGGAGTCCC AATTCAGGTC TTATTTGTCC TCCTTCTGAA 
ATGCCAGCAA CGCCTCACAG TGGAGATTTG ATGGACTCTA TTTCTCAGCA GAAATCAGAA 
ACGATACCAT CTGAGATTAC CAACACAAAG AAAGGAAAAA GTCACAAAAG TCAGTCATCA 
ATGGCCCATA AGGGTGAAAT CCATGACCCT CCTCCAGAGG CAGTTCAAAC TGTAACTCCT 
GTGGAGGTTC ACATAGGCAC CTGGTTTACA ACCACACCTG AAGGAAATCG GATCGGCACC 
AAAGGATTAG AAAGAATAGC AGACTTGACC CCATTGTTAT CCTTTCAGGC CACAGATCCT 
GTCAATGGAA CGGTTATGAC AACTCGAGAA GACAAAGTTG TCATAGTTGA AAGGAAAGAT 
GGTACTCGGA TAGTGGATCA TGCTGATGGT ACCAGAATCA CAACCTTTTA TCAAGTTTAT 
GAAGATCAAA TTATTCTGCC AGATGATCAA GAAACAACCG AGGGTCCTCG GACTGTCACC 
AGGCAGGTGA AGTGTATGCG GGTAGAAAGC TCACGCTATG CCACTGTTAT CGCCAACTGT 
GAGGACAGTA GCTGCTGTGC CACCTTTGGA GATGGAACAA CTATTATTGC AAAGCCACAG 
GGAACATACC AGGTGTTACC TCCAAACACA GGCTCTCTTT ATATTGACAA GGATTGTTCA 
GCTGTGTACT GCCATGAGTC AAGCAGTAAT ATATACTATC CTTTTCAAAA GCGTGAGCAG 
CTGCGAGCTG GCAGGTACAT CATGAGGCAT ACTTCAGAGG TTATCTGTGA GGTTCTGGAT 
CCTGAGGGAA ACACTTTTCA GGTCATGGCT GATGGTAGCA TATCAACTAT ATTACCTGAA 
AAAAAATTGG AAGATGATTT AAATGAGAAA ACTGAGGGCT ATGATAGTCT GTCCTCTATG 
CACCTTGAAA AGAATCATCA GCAAATCTAT GGTGAACATG TCCCCAGGTT TTTTGTTATG 
TATGCTGATG GATCAGGAAT GGAACTTCTT CGAGACAGTG ACATAGAAGA ATATCTATCT 
TTGGCATATA AAGAATCAAA TACTGTTGTT CTCCAAGAGC CAGTGCAGGA ACAGCCAGGC 
ACCCTAACCA TCACAGTCCT TCGCCCTTTC CATGAAGCAT CACCATGGCA AGTAAAAAAG 
GAAGATACAA TTGTCCCTCC TAATCTCCGG TCAAGGTCAT GGGAAACATT TCCCTCAGTT 
GAGAAAAAAA CTCCAGGACC TCCGTTTGGT ACTCAGATTT GGAAAGGCCT TTGCATTGAG 
TCCAAACAGC TAGTGAGTGC CCCGGGTGCC ATACTCAAGA GCCCCAGTGT GCTACAGATG 
CGCCAATTCA TTCAGCATGA GGTCATAAAG AATGAGGTGA AACTGAGGCT GCAGGTTTCC 
CTTAAGGATT ACATAAACTA TATTCTAAAG AAAGAAGATG AGCTGCAGGA AATGATGGTT 
AAAGATTCCA GAACTGAGGA GGAGAGAGGC AATGCTGCTG ATCTCCTCAA GCTGGTTATG 
TCTTTCCCTA AAATGGAGGA AACTACAAAA AGTCATGTTA CTGAAGTTGC AGCTCACCTA 
ACTGATTTAT TCAAGCAGTC TTTGGCTACG CCTCCAAAAT GCCCACCAGA CACATTTGGT 
AAAGATTTCT TTGAAAAGAC ATGGAGACAC ACAGCATCCT CAAAACGCTG GAAAGAAAAG 
ATAGACAAAA CGAGGAAGGA AATTGAGACA ACACAGAATT ACCTAATGGA TATTAAGAAC 
CGCATAATAC CACCCTTTTT TAAATCTGAA TTGAACCAGT TATATCAGTC TCAGTATAAT 
CACCTGGACA GTCTTTCCAA AAAACTGCCT TCTTTTACAA AGAAAAATGA AGATGCAAAC 
GAAACAGCTG TTCAAGATAC ATCTGATCTT AATCTAGATT TCAAGCCACA TAAGGTTTCA 
GAACAGAAAT CCTCAAGTGT GCCTAGTCTT CCAAAACCAG AGATTTCTGC AGATAAGAAG 
GATTTCACTG CTCAGAACCA AACTGAAAAT TTAACAAAAT CTCCTGAAGA AGCAGAATCT 
TATGAGCCCG TGAAAATTCC AACCCAGTCC TTGCTGCAGG ATGTTGCGGG ACAAACAAGA 
AAAGAAAAAG TGAAGTTGCC TCATTATTTG CTGAGTTCCA AGCCTAAGTC TCAACCTCTT 
GCAAAGGTGC AAGATTCTGT TGGAGGAAAA GTGAACACAT CCTCTGTTGC ATCTGCTGCC 
ATTAATAATG CAAAGTCATC CCTTTTTGGG TTCCATCTTC TCCCATCATC AGTCAAGTTT 
GGAGTGCTTA AGGAAGGACA TACCTATGCC ACAGTTGTAA AGCTCAAGAA TGTTGGAGTG 
GACTTCTGCA GGTTTAAAGT AAAGCAGCCC CCACCCAGCA CAGGACTGAA AGTGACTTAC 
AAACCTGGAC CTGTGGCAGC TGGTATGCAG ACAGAACTGA ATATAGAGTT ATTTGCCACA 
GCTGTTGGAG AGGATGGGGC CAAGGGATCA GCACACATCT CTCACAATAT CGAGATTATG 
ACAGAGCATG AGGTTCTGTT CCTACCTGTG GAAGCAACTG TTTTAACAAG CAGCAATTAT 
GATAAACGAC CAAAAGACTT TCCCCAGGGA AAAGAAAATC CAATGGTCCA GAGAACTTCT 
ACAATTTATT CCTCCACACT TGGAGTCTTC ATGTCTCGTA AAGTTTCTCC ACATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPAG17 ( XM_011540934.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011539236.1 Homo sapiens sperm associated antigen 17 (SPAG17), transcript variant X3, mRNA.

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CloneID	OHu77634
Clone ID Related Accession (Same CDS sequence)	XM_011540934.1
Accession Version	XM_011540934.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6540bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	sperm-associated antigen 17 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCACCCA AGAAGGAGAA AGGAGGAACT GTGAACACCA GTTCTAAGAT ATGGGAACCC 
TCGCTCATAG CTGCACAGTT CAATCAGAAC GATTGGCAGG CCTCCATTGC TTTTGTGGTT 
GGGAACCAGA TTGAAGATGA TCTTCTCATC CAAGCCCTTA CCGTGGCTGT CCAGGTCCCT 
CAGCGTAAAC TCTTCAGTAT GGTGTCGTGG CAAGACATTC TCCAGCAGAT TAATGAAATA 
AATACACTTG TTGGATCTGC TTCATCTAAA AAGGCAAAAA AACCTGTAGG TGGTAATGCT 
CCTTTATATT ATGAGGTGTT AACGGCAGCA AAAGCAATTA TGGATAGTGG AGAGAAATTA 
ACCTTACCAC TGATAGGGAA ACTCTTGAAA TTTCAACTTC TCCAGATTAA ATTTAAGGAC 
CAACAGCGAC GGGAAAATGA AAAGAAGGTA ATAGAAGACA AACCTAAGTT AGAAAAGGAT 
AAAGGGAAAG CAAAATCTCC CAAGGAGAAA AAGGCTCCAA GTGCCAAGCC TGCCAAAGGA 
AAGGGAAAGG ATCAGCCTGA GGCAAATGCA CCAGTGAAAA AGACCACCCA GTTAAAGCGG 
AGAGGAGAAG ACGACCACAC CAATCGTTAC ATTGACGATG AGCCAGATGA TGGTGCCCAA 
CATTACATTA TAGTTGTGGG CTTTAACAAT CCTCAGCTAT TAGCAATTAT GGCTGAGCTT 
GGCATTCCTA TAACCAGCGT GATTAAAATA TCTTCAGAGA ATTATGAACC TCTGCAGACA 
CACCTGGCAG CAGTTAACCA GCAGCAGGAA GTTCTTCTTC AGTCAGAAGA TCTAGAAGCA 
GAAAAATTGA AGAAAGAAAA TGCCATAAAA GAGCTTAAAA CTTTCTGGAA GTACTTGGAA 
CCAGTCCTGA ATAATGAGAA ACCTGAAACA AATCTCTTTG ATGTTGCTCG ACTTGAGTAC 
ATGGTCAAAG CAGCTGATTT TCCTTCTGAC TGGTCAGATG GTGAGATGAT GCTGAAATTG 
GGCACTGATA TTTTTGAAAA TATTGCCTGC TTGATGTATG ACATCCTGGA TTGGAAAAGG 
CAGCACCAGC ACTATTTGGA AAGCATGCAG CTTATTAATG TTCCACAAGT GGTTAATGAG 
AAACCTGTAT TAGAAGCCAT GCCAACTTCA GAGGCTCCAC AACCTGCTGT ACCAGCTCCT 
GGAAAGAAGA AAGCACAGTA TGAAGAACCG CAAGCTCCAC CACCAGTGAC TTCAGTCATC 
ACAACTGAAG TAGACATGAG ATATTACAAT TATTTGCTGA ATCCAATTCG AGAGGAATTC 
ATTTCTGTGC CCCTGATACT GCATTGTATG CTGGAACAGG TTGTTGCAAC TGAAGAAGAT 
CTCGTCCCAC CCAGTCTGCG GGAGCCATCC CCCAGAGCAG ACGGGCTAGA CCACAGAATC 
GCAGCTCACA TTGTGTCCCT TCTGCCCTCA CTCTGTCTCT CAGAGAGGGA GAAAAAGAAT 
CTTCATGACA TATTTTTATC TGAAGAAGAA AATGAAAGCA AAGCAGTGCC CAAAGGCCCC 
CTCCTACTGA ACTATCATGA TGCACACGCC CACAAGAAGT ACGCACTACA GGACCAAAAG 
AATTTTGATC CAGTTCAAAT TGAGCAGGAG ATGCAGTCCA AGTTGCCACT GTGGGAATTT 
CTTCAATTCC CTCTACCCCC ACCATGGAAC AACACTAAAC GTCTAGCTAC AATTCATGAG 
CTTATGCACT TTTGTACGAG TGATGTCTTG AGCTGGAATG AAGTAGAACG AGCCTTCAAG 
GTGTTTACTT TTGAGAGCCT GAAGCTCTCT GAGGTTGATG AAAAAGGGAA ACTGAAACCT 
TCTGGGATGA TGTGTGGGTC AGATTCTGAA ATGTTCAACA TACCGTGGGA CAACCCTGCC 
AGATTTGCTA AACAGATAAG GCAGCAATAT GTCATGAAAA TGAATACTCA AGAGGCCAAG 
CAGAAAGCAG ATATTAAAAT CAAAGACAGA ACACTATTTG TGGATCAGAA TTTGTCAATG 
TCTGTGCAAG ATAATGAAAG CAACCGAGAA CCTTCAGATC CTAGTCAGTG TGATGCTAAC 
AATATGAAGC ATTCTGACTT GAATAATCTC AAACTCTCAG TCCCTGATAA TAGACAGCTG 
TTAGAGCAGG AGAGCATCAT GAAGGCTCAG CCCCAACATG AGTCTCTGGA GCAGACCACA 
AACAATGAGA TCAAAGATGA TGCAGTCACA AAGGCTGATT CTCATGAAAA GAAACCCAAG 
AAGATGATGG TGGAAGCAGA TTTAGAGGAC ATAAAGAAAA CACAGCAGCG CAGTCTAATG 
GACTGGAGTT TTACTGAACA TTTTAAACCG AAAGTACTGC TTCAGGTCCT TCAAGAAGCC 
CATAAGCAAT ATAGGTGTGT TGATTCTTAC TACCACACCC AAGACAACTC TTTACTTTTA 
GTCTTTCACA ATCCAATGAA TAGACAACGT TTGCATTGTG AATATTGGAA CATTGCTCTC 
CACTCCAATG TTGGATTCAG GAATTATTTG GAACTTGTTG CAAAATCTAT TCAAGATTGG 
ATTACAAAAG AAGAAGCTAT ATATCAGGAA TCTAAAATGA ATGAGAAAAT CATCAGGACC 
AGAGCTGAGC TGGAATTGAA ATCTTCTGCT AATGCCAAAC TTACTTCTGC TAGCAAAATT 
TTTTCCATTA AAGAATCTAA AAGTAACAAA GGAATCAGCA AAACAGAGAT ATCAGATCAA 
GAAAAAGAAA AAGAGAAGGA AAAGATTCCT TTCATTTTAG AAGGCTCTCT CAAGGCATGG 
AAAGAAGAGC AACATCGATT AGCAGAAGAG GAGCGCTTAA GGGAAGAAAA GAAAGCAGAG 
AAGAAGGGTA AAGAAGCTGG TAAAAAGAAA GGCAAGGATA ACGCAGAGAA AGAGGATAGT 
AGGTCTTTGA AGAAAAAATC ACCTTACAAG GAGAAATCTA AAGAAGAACA AGTCAAGATC 
CAAGAAGTAA CAGAAGAGTC CCCCCACCAA CCAGAACCTA AGATAACTTA CCCGTTTCAC 
GGATACAATA TGGGAAATAT ACCCACTCAA ATCTCAGGGT CAAATTACTA CCTGTATCCT 
TCTGATGGGG GGCAGATTGA AGTGGAAAAG ACAATGTTTG AAAAAGGCCC AACTTTTATC 
AAAGTGAGAG TGGTAAAGGA CAACCACAAT TTTATGATTC ATTTAAATGA CCCTAAGGAA 
ATTGTGAAAA AGGAAGAGAA AGGGGATTAT TATTTAGAAG AGGAAGAAGA AGGAGATGAG 
GAACAAAGTC TTGAAACGGA AGTATCAGAT GCAAAGAATA AAGCTTTCAG CAAGTTTGGA 
TCTTTTTCTG CCACCTTAGA AAATGGAATC TGCCTCTCGA TAAGTTACTA TGGATCAAAT 
GGAATGGCAC CAGAAGATAA GGATCCTGAT TTAGAAACAA TATTGAATAT CCCTTCAGCA 
CTCACTCCAA CAGTGGTTCC TGTTATAGTG ACCGTTCCTC AAAGCAAAGC TAAAGGGAAA 
ATAAAAGGCA AAGAAAAACC CAAAGAATCC CTTAAAGAAG AAGAACACCC AAAAGAAGAA 
GAGAAAAAGG AAGAAGAAGT AGAACCAGAA CCTGTTTTAC AAGAGACTTT GGATGTTCCC 
ACCTTCCAGA GCCTAAATGT GTCTTGCCCC AGTGGGCTCC TGTTGACTTT CATTGGACAA 
GAATCTACAG GTCAATATGT TATAGATGAG GAACCCACCT GGGACATCAT GGTCCGTCAG 
AGCTACCCCC AGAGGGTGAA GCACTATGAG TTCTATAAAA CGGTGATGCC ACCCGCAGAG 
CAGGAGGCTT CAAGGGTTAT CACCAGTCAA GGCACTGTTG TCAAATATAT GTTGGATGGA 
TCCACACAGA TTCTCTTTGC AGATGGTGCT GTGAGCAGGA GTCCCAATTC AGGTCTTATT 
TGTCCTCCTT CTGAAATGCC AGCAACGCCT CACAGTGGAG ATTTGATGGA CTCTATTTCT 
CAGCAGAAAT CAGAAACGAT ACCATCTGAG ATTACCAACA CAAAGAAAGG AAAAAGTCAC 
AAAAGTCAGT CATCAATGGC CCATAAGGGT GAAATCCATG ACCCTCCTCC AGAGGCAGTT 
CAAACTGTAA CTCCTGTGGA GGTTCACATA GGCACCTGGT TTACAACCAC ACCTGAAGGA 
AATCGGATCG GCACCAAAGG ATTAGAAAGA ATAGCAGACT TGACCCCATT GTTATCCTTT 
CAGGCCACAG ATCCTGTCAA TGGAACGGTT ATGACAACTC GAGAAGACAA AGTTGTCATA 
GTTGAAAGGA AAGATGGTAC TCGGATAGTG GATCATGCTG ATGGTACCAG AATCACAACC 
TTTTATCAAG TTTATGAAGA TCAAATTATT CTGCCAGATG ATCAAGAAAC AACCGAGGGT 
CCTCGGACTG TCACCAGGCA GGTGAAGTGT ATGCGGGTAG AAAGCTCACG CTATGCCACT 
GTTATCGCCA ACTGTGAGGA CAGTAGCTGC TGTGCCACCT TTGGAGATGG AACAACTATT 
ATTGCAAAGC CACAGGGAAC ATACCAGGTG TTACCTCCAA ACACAGGCTC TCTTTATATT 
GACAAGGATT GTTCAGCTGT GTACTGCCAT GAGTCAAGCA GTAATATATA CTATCCTTTT 
CAAAAGCGTG AGCAGCTGCG AGCTGGCAGG TACATCATGA GGCATACTTC AGAGGTTATC 
TGTGAGGTTC TGGATCCTGA GGGAAACACT TTTCAGGTCA TGGCTGATGG TAGCATATCA 
ACTATATTAC CTGAAAAAAA ATTGGAAGAT GATTTAAATG AGAAAACTGA GGGCTATGAT 
AGTCTGTCCT CTATGCACCT TGAAAAGAAT CATCAGCAAA TCTATGGTGA ACATGTCCCC 
AGGTTTTTTG TTATGTATGC TGATGGATCA GGAATGGAAC TTCTTCGAGA CAGTGACATA 
GAAGAATATC TATCTTTGGC ATATAAAGAA TCAAATACTG TTGTTCTCCA AGAGCCAGTG 
CAGGAACAGC CAGGCACCCT AACCATCACA GTCCTTCGCC CTTTCCATGA AGCATCACCA 
TGGCAAGTAA AAAAGGAAGA TACAATTGTC CCTCCTAATC TCCGGTCAAG GTCATGGGAA 
ACATTTCCCT CAGTTGAGAA AAAAACTCCA GGACCTCCGT TTGGTACTCA GATTTGGAAA 
GGCCTTTGCA TTGAGTCCAA ACAGCTAGTG AGTGCCCCGG GTGCCATACT CAAGAGCCCC 
AGTGTGCTAC AGATGCGCCA ATTCATTCAG CATGAGGTCA TAAAGAATGA GGTGAAACTG 
AGGCTGCAGG TTTCCCTTAA GGATTACATA AACTATATTC TAAAGAAAGA AGATGAGCTG 
CAGGAAATGA TGGTTAAAGA TTCCAGAACT GAGGAGGAGA GAGGCAATGC TGCTGATCTC 
CTCAAGCTGG TTATGTCTTT CCCTAAAATG GAGGAAACTA CAAAAAGTCA TGTTACTGAA 
GTTGCAGCTC ACCTAACTGA TTTATTCAAG CAGTCTTTGG CTACGCCTCC AAAATGCCCA 
CCAGACACAT TTGGTAAAGA TTTCTTTGAA AAGACATGGA GACACACAGC ATCCTCAAAA 
CGCTGGAAAG AAAAGATAGA CAAAACGAGG AAGGAAATTG AGACAACACA GAATTACCTA 
ATGGATATTA AGAACCGCAT AATACCACCC TTTTTTAAAT CTGAATTGAA CCAGTTATAT 
CAGTCTCAGT ATAATCACCT GGACAGTCTT TCCAAAAAAC TGCCTTCTTT TACAAAGAAA 
AATGAAGATG CAAACGAAAC AGCTGTTCAA GATACATCTG ATCTTAATCT AGAAGCAGAA 
TCTTATGAGC CCGTGAAAAT TCCAACCCAG TCCTTGCTGC AGGATGTTGC GGGACAAACA 
AGAAAAGAAA AAGTGAAGTT GCCTCATTAT TTGCTGAGTT CCAAGCCTAA GTCTCAACCT 
CTTGCAAAGG TGCAAGATTC TGTTGGAGGA AAAGTGAACA CATCCTCTGT TGCATCTGCT 
GCCATTAATA ATGCAAAGTC ATCCCTTTTT GGGTTCCATC TTCTCCCATC ATCAGTCAAG 
TTTGGAGTGC TTAAGGAAGG ACATACCTAT GCCACAGTTG TAAAGCTCAA GAATGTTGGA 
GTGGACTTCT GCAGGTTTAA AGTAAAGCAG CCCCCACCCA GCACAGGACT GAAAGTGACT 
TACAAACCTG GACCTGTGGC AGCTGGTATG CAGACAGAAC TGAATATAGA GTTATTTGCC 
ACAGCTGTTG GAGAGGATGG GGCCAAGGGA TCAGCACACA TCTCTCACAA TATCGAGATT 
ATGACAGAGC ATGAGGTTCT GTTCCTACCT GTGGAAGCAA CTGTTTTAAC AAGCAGCAAT 
TATGATAAAC GACCAAAAGA CTTTCCCCAG GGAAAAGAAA ATCCAATGGT CCAGAGAACT 
TCTACAATTT ATTCCTCCAC ACTTGGAGTC TTCATGTCTC GTAAAGTTTC TCCACATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011539236.1
CDS	69..6608
Translation	MAPKKEKGGTVNTSSKIWEPSLIAAQFNQNDWQASIAFVVGNQIEDDLLIQALTVAVQVPQRKLFSMVSWQDILQQINEINTLVGSASSKKAKKPVGGNAPLYYEVLTAAKAIMDSGEKLTLPLIGKLLKFQLLQIKFKDQQRRENEKKVIEDKPKLEKDKGKAKSPKEKKAPSAKPAKGKGKDQPEANAPVKKTTQLKRRGEDDHTNRYIDDEPDDGAQHYIIVVGFNNPQLLAIMAELGIPITSVIKISSENYEPLQTHLAAVNQQQEVLLQSEDLEAEKLKKENAIKELKTFWKYLEPVLNNEKPETNLFDVARLEYMVKAADFPSDWSDGEMMLKLGTDIFENIACLMYDILDWKRQHQHYLESMQLINVPQVVNEKPVLEAMPTSEAPQPAVPAPGKKKAQYEEPQAPPPVTSVITTEVDMRYYNYLLNPIREEFISVPLILHCMLEQVVATEEDLVPPSLREPSPRADGLDHRIAAHIVSLLPSLCLSEREKKNLHDIFLSEEENESKAVPKGPLLLNYHDAHAHKKYALQDQKNFDPVQIEQEMQSKLPLWEFLQFPLPPPWNNTKRLATIHELMHFCTSDVLSWNEVERAFKVFTFESLKLSEVDEKGKLKPSGMMCGSDSEMFNIPWDNPARFAKQIRQQYVMKMNTQEAKQKADIKIKDRTLFVDQNLSMSVQDNESNREPSDPSQCDANNMKHSDLNNLKLSVPDNRQLLEQESIMKAQPQHESLEQTTNNEIKDDAVTKADSHEKKPKKMMVEADLEDIKKTQQRSLMDWSFTEHFKPKVLLQVLQEAHKQYRCVDSYYHTQDNSLLLVFHNPMNRQRLHCEYWNIALHSNVGFRNYLELVAKSIQDWITKEEAIYQESKMNEKIIRTRAELELKSSANAKLTSASKIFSIKESKSNKGISKTEISDQEKEKEKEKIPFILEGSLKAWKEEQHRLAEEERLREEKKAEKKGKEAGKKKGKDNAEKEDSRSLKKKSPYKEKSKEEQVKIQEVTEESPHQPEPKITYPFHGYNMGNIPTQISGSNYYLYPSDGGQIEVEKTMFEKGPTFIKVRVVKDNHNFMIHLNDPKEIVKKEEKGDYYLEEEEEGDEEQSLETEVSDAKNKAFSKFGSFSATLENGICLSISYYGSNGMAPEDKDPDLETILNIPSALTPTVVPVIVTVPQSKAKGKIKGKEKPKESLKEEEHPKEEEKKEEEVEPEPVLQETLDVPTFQSLNVSCPSGLLLTFIGQESTGQYVIDEEPTWDIMVRQSYPQRVKHYEFYKTVMPPAEQEASRVITSQGTVVKYMLDGSTQILFADGAVSRSPNSGLICPPSEMPATPHSGDLMDSISQQKSETIPSEITNTKKGKSHKSQSSMAHKGEIHDPPPEAVQTVTPVEVHIGTWFTTTPEGNRIGTKGLERIADLTPLLSFQATDPVNGTVMTTREDKVVIVERKDGTRIVDHADGTRITTFYQVYEDQIILPDDQETTEGPRTVTRQVKCMRVESSRYATVIANCEDSSCCATFGDGTTIIAKPQGTYQVLPPNTGSLYIDKDCSAVYCHESSSNIYYPFQKREQLRAGRYIMRHTSEVICEVLDPEGNTFQVMADGSISTILPEKKLEDDLNEKTEGYDSLSSMHLEKNHQQIYGEHVPRFFVMYADGSGMELLRDSDIEEYLSLAYKESNTVVLQEPVQEQPGTLTITVLRPFHEASPWQVKKEDTIVPPNLRSRSWETFPSVEKKTPGPPFGTQIWKGLCIESKQLVSAPGAILKSPSVLQMRQFIQHEVIKNEVKLRLQVSLKDYINYILKKEDELQEMMVKDSRTEEERGNAADLLKLVMSFPKMEETTKSHVTEVAAHLTDLFKQSLATPPKCPPDTFGKDFFEKTWRHTASSKRWKEKIDKTRKEIETTQNYLMDIKNRIIPPFFKSELNQLYQSQYNHLDSLSKKLPSFTKKNEDANETAVQDTSDLNLEAESYEPVKIPTQSLLQDVAGQTRKEKVKLPHYLLSSKPKSQPLAKVQDSVGGKVNTSSVASAAINNAKSSLFGFHLLPSSVKFGVLKEGHTYATVVKLKNVGVDFCRFKVKQPPPSTGLKVTYKPGPVAAGMQTELNIELFATAVGEDGAKGSAHISHNIEIMTEHEVLFLPVEATVLTSSNYDKRPKDFPQGKENPMVQRTSTIYSSTLGVFMSRKVSPH

Target ORF information:

RefSeq Version	XM_011540934.1
Organism	Homo sapiens(human)
Definition	Homo sapiens sperm associated antigen 17 (SPAG17), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011540934.1

ORF Insert Sequence:

ATGGCACCCA AGAAGGAGAA AGGAGGAACT GTGAACACCA GTTCTAAGAT ATGGGAACCC 
TCGCTCATAG CTGCACAGTT CAATCAGAAC GATTGGCAGG CCTCCATTGC TTTTGTGGTT 
GGGAACCAGA TTGAAGATGA TCTTCTCATC CAAGCCCTTA CCGTGGCTGT CCAGGTCCCT 
CAGCGTAAAC TCTTCAGTAT GGTGTCGTGG CAAGACATTC TCCAGCAGAT TAATGAAATA 
AATACACTTG TTGGATCTGC TTCATCTAAA AAGGCAAAAA AACCTGTAGG TGGTAATGCT 
CCTTTATATT ATGAGGTGTT AACGGCAGCA AAAGCAATTA TGGATAGTGG AGAGAAATTA 
ACCTTACCAC TGATAGGGAA ACTCTTGAAA TTTCAACTTC TCCAGATTAA ATTTAAGGAC 
CAACAGCGAC GGGAAAATGA AAAGAAGGTA ATAGAAGACA AACCTAAGTT AGAAAAGGAT 
AAAGGGAAAG CAAAATCTCC CAAGGAGAAA AAGGCTCCAA GTGCCAAGCC TGCCAAAGGA 
AAGGGAAAGG ATCAGCCTGA GGCAAATGCA CCAGTGAAAA AGACCACCCA GTTAAAGCGG 
AGAGGAGAAG ACGACCACAC CAATCGTTAC ATTGACGATG AGCCAGATGA TGGTGCCCAA 
CATTACATTA TAGTTGTGGG CTTTAACAAT CCTCAGCTAT TAGCAATTAT GGCTGAGCTT 
GGCATTCCTA TAACCAGCGT GATTAAAATA TCTTCAGAGA ATTATGAACC TCTGCAGACA 
CACCTGGCAG CAGTTAACCA GCAGCAGGAA GTTCTTCTTC AGTCAGAAGA TCTAGAAGCA 
GAAAAATTGA AGAAAGAAAA TGCCATAAAA GAGCTTAAAA CTTTCTGGAA GTACTTGGAA 
CCAGTCCTGA ATAATGAGAA ACCTGAAACA AATCTCTTTG ATGTTGCTCG ACTTGAGTAC 
ATGGTCAAAG CAGCTGATTT TCCTTCTGAC TGGTCAGATG GTGAGATGAT GCTGAAATTG 
GGCACTGATA TTTTTGAAAA TATTGCCTGC TTGATGTATG ACATCCTGGA TTGGAAAAGG 
CAGCACCAGC ACTATTTGGA AAGCATGCAG CTTATTAATG TTCCACAAGT GGTTAATGAG 
AAACCTGTAT TAGAAGCCAT GCCAACTTCA GAGGCTCCAC AACCTGCTGT ACCAGCTCCT 
GGAAAGAAGA AAGCACAGTA TGAAGAACCG CAAGCTCCAC CACCAGTGAC TTCAGTCATC 
ACAACTGAAG TAGACATGAG ATATTACAAT TATTTGCTGA ATCCAATTCG AGAGGAATTC 
ATTTCTGTGC CCCTGATACT GCATTGTATG CTGGAACAGG TTGTTGCAAC TGAAGAAGAT 
CTCGTCCCAC CCAGTCTGCG GGAGCCATCC CCCAGAGCAG ACGGGCTAGA CCACAGAATC 
GCAGCTCACA TTGTGTCCCT TCTGCCCTCA CTCTGTCTCT CAGAGAGGGA GAAAAAGAAT 
CTTCATGACA TATTTTTATC TGAAGAAGAA AATGAAAGCA AAGCAGTGCC CAAAGGCCCC 
CTCCTACTGA ACTATCATGA TGCACACGCC CACAAGAAGT ACGCACTACA GGACCAAAAG 
AATTTTGATC CAGTTCAAAT TGAGCAGGAG ATGCAGTCCA AGTTGCCACT GTGGGAATTT 
CTTCAATTCC CTCTACCCCC ACCATGGAAC AACACTAAAC GTCTAGCTAC AATTCATGAG 
CTTATGCACT TTTGTACGAG TGATGTCTTG AGCTGGAATG AAGTAGAACG AGCCTTCAAG 
GTGTTTACTT TTGAGAGCCT GAAGCTCTCT GAGGTTGATG AAAAAGGGAA ACTGAAACCT 
TCTGGGATGA TGTGTGGGTC AGATTCTGAA ATGTTCAACA TACCGTGGGA CAACCCTGCC 
AGATTTGCTA AACAGATAAG GCAGCAATAT GTCATGAAAA TGAATACTCA AGAGGCCAAG 
CAGAAAGCAG ATATTAAAAT CAAAGACAGA ACACTATTTG TGGATCAGAA TTTGTCAATG 
TCTGTGCAAG ATAATGAAAG CAACCGAGAA CCTTCAGATC CTAGTCAGTG TGATGCTAAC 
AATATGAAGC ATTCTGACTT GAATAATCTC AAACTCTCAG TCCCTGATAA TAGACAGCTG 
TTAGAGCAGG AGAGCATCAT GAAGGCTCAG CCCCAACATG AGTCTCTGGA GCAGACCACA 
AACAATGAGA TCAAAGATGA TGCAGTCACA AAGGCTGATT CTCATGAAAA GAAACCCAAG 
AAGATGATGG TGGAAGCAGA TTTAGAGGAC ATAAAGAAAA CACAGCAGCG CAGTCTAATG 
GACTGGAGTT TTACTGAACA TTTTAAACCG AAAGTACTGC TTCAGGTCCT TCAAGAAGCC 
CATAAGCAAT ATAGGTGTGT TGATTCTTAC TACCACACCC AAGACAACTC TTTACTTTTA 
GTCTTTCACA ATCCAATGAA TAGACAACGT TTGCATTGTG AATATTGGAA CATTGCTCTC 
CACTCCAATG TTGGATTCAG GAATTATTTG GAACTTGTTG CAAAATCTAT TCAAGATTGG 
ATTACAAAAG AAGAAGCTAT ATATCAGGAA TCTAAAATGA ATGAGAAAAT CATCAGGACC 
AGAGCTGAGC TGGAATTGAA ATCTTCTGCT AATGCCAAAC TTACTTCTGC TAGCAAAATT 
TTTTCCATTA AAGAATCTAA AAGTAACAAA GGAATCAGCA AAACAGAGAT ATCAGATCAA 
GAAAAAGAAA AAGAGAAGGA AAAGATTCCT TTCATTTTAG AAGGCTCTCT CAAGGCATGG 
AAAGAAGAGC AACATCGATT AGCAGAAGAG GAGCGCTTAA GGGAAGAAAA GAAAGCAGAG 
AAGAAGGGTA AAGAAGCTGG TAAAAAGAAA GGCAAGGATA ACGCAGAGAA AGAGGATAGT 
AGGTCTTTGA AGAAAAAATC ACCTTACAAG GAGAAATCTA AAGAAGAACA AGTCAAGATC 
CAAGAAGTAA CAGAAGAGTC CCCCCACCAA CCAGAACCTA AGATAACTTA CCCGTTTCAC 
GGATACAATA TGGGAAATAT ACCCACTCAA ATCTCAGGGT CAAATTACTA CCTGTATCCT 
TCTGATGGGG GGCAGATTGA AGTGGAAAAG ACAATGTTTG AAAAAGGCCC AACTTTTATC 
AAAGTGAGAG TGGTAAAGGA CAACCACAAT TTTATGATTC ATTTAAATGA CCCTAAGGAA 
ATTGTGAAAA AGGAAGAGAA AGGGGATTAT TATTTAGAAG AGGAAGAAGA AGGAGATGAG 
GAACAAAGTC TTGAAACGGA AGTATCAGAT GCAAAGAATA AAGCTTTCAG CAAGTTTGGA 
TCTTTTTCTG CCACCTTAGA AAATGGAATC TGCCTCTCGA TAAGTTACTA TGGATCAAAT 
GGAATGGCAC CAGAAGATAA GGATCCTGAT TTAGAAACAA TATTGAATAT CCCTTCAGCA 
CTCACTCCAA CAGTGGTTCC TGTTATAGTG ACCGTTCCTC AAAGCAAAGC TAAAGGGAAA 
ATAAAAGGCA AAGAAAAACC CAAAGAATCC CTTAAAGAAG AAGAACACCC AAAAGAAGAA 
GAGAAAAAGG AAGAAGAAGT AGAACCAGAA CCTGTTTTAC AAGAGACTTT GGATGTTCCC 
ACCTTCCAGA GCCTAAATGT GTCTTGCCCC AGTGGGCTCC TGTTGACTTT CATTGGACAA 
GAATCTACAG GTCAATATGT TATAGATGAG GAACCCACCT GGGACATCAT GGTCCGTCAG 
AGCTACCCCC AGAGGGTGAA GCACTATGAG TTCTATAAAA CGGTGATGCC ACCCGCAGAG 
CAGGAGGCTT CAAGGGTTAT CACCAGTCAA GGCACTGTTG TCAAATATAT GTTGGATGGA 
TCCACACAGA TTCTCTTTGC AGATGGTGCT GTGAGCAGGA GTCCCAATTC AGGTCTTATT 
TGTCCTCCTT CTGAAATGCC AGCAACGCCT CACAGTGGAG ATTTGATGGA CTCTATTTCT 
CAGCAGAAAT CAGAAACGAT ACCATCTGAG ATTACCAACA CAAAGAAAGG AAAAAGTCAC 
AAAAGTCAGT CATCAATGGC CCATAAGGGT GAAATCCATG ACCCTCCTCC AGAGGCAGTT 
CAAACTGTAA CTCCTGTGGA GGTTCACATA GGCACCTGGT TTACAACCAC ACCTGAAGGA 
AATCGGATCG GCACCAAAGG ATTAGAAAGA ATAGCAGACT TGACCCCATT GTTATCCTTT 
CAGGCCACAG ATCCTGTCAA TGGAACGGTT ATGACAACTC GAGAAGACAA AGTTGTCATA 
GTTGAAAGGA AAGATGGTAC TCGGATAGTG GATCATGCTG ATGGTACCAG AATCACAACC 
TTTTATCAAG TTTATGAAGA TCAAATTATT CTGCCAGATG ATCAAGAAAC AACCGAGGGT 
CCTCGGACTG TCACCAGGCA GGTGAAGTGT ATGCGGGTAG AAAGCTCACG CTATGCCACT 
GTTATCGCCA ACTGTGAGGA CAGTAGCTGC TGTGCCACCT TTGGAGATGG AACAACTATT 
ATTGCAAAGC CACAGGGAAC ATACCAGGTG TTACCTCCAA ACACAGGCTC TCTTTATATT 
GACAAGGATT GTTCAGCTGT GTACTGCCAT GAGTCAAGCA GTAATATATA CTATCCTTTT 
CAAAAGCGTG AGCAGCTGCG AGCTGGCAGG TACATCATGA GGCATACTTC AGAGGTTATC 
TGTGAGGTTC TGGATCCTGA GGGAAACACT TTTCAGGTCA TGGCTGATGG TAGCATATCA 
ACTATATTAC CTGAAAAAAA ATTGGAAGAT GATTTAAATG AGAAAACTGA GGGCTATGAT 
AGTCTGTCCT CTATGCACCT TGAAAAGAAT CATCAGCAAA TCTATGGTGA ACATGTCCCC 
AGGTTTTTTG TTATGTATGC TGATGGATCA GGAATGGAAC TTCTTCGAGA CAGTGACATA 
GAAGAATATC TATCTTTGGC ATATAAAGAA TCAAATACTG TTGTTCTCCA AGAGCCAGTG 
CAGGAACAGC CAGGCACCCT AACCATCACA GTCCTTCGCC CTTTCCATGA AGCATCACCA 
TGGCAAGTAA AAAAGGAAGA TACAATTGTC CCTCCTAATC TCCGGTCAAG GTCATGGGAA 
ACATTTCCCT CAGTTGAGAA AAAAACTCCA GGACCTCCGT TTGGTACTCA GATTTGGAAA 
GGCCTTTGCA TTGAGTCCAA ACAGCTAGTG AGTGCCCCGG GTGCCATACT CAAGAGCCCC 
AGTGTGCTAC AGATGCGCCA ATTCATTCAG CATGAGGTCA TAAAGAATGA GGTGAAACTG 
AGGCTGCAGG TTTCCCTTAA GGATTACATA AACTATATTC TAAAGAAAGA AGATGAGCTG 
CAGGAAATGA TGGTTAAAGA TTCCAGAACT GAGGAGGAGA GAGGCAATGC TGCTGATCTC 
CTCAAGCTGG TTATGTCTTT CCCTAAAATG GAGGAAACTA CAAAAAGTCA TGTTACTGAA 
GTTGCAGCTC ACCTAACTGA TTTATTCAAG CAGTCTTTGG CTACGCCTCC AAAATGCCCA 
CCAGACACAT TTGGTAAAGA TTTCTTTGAA AAGACATGGA GACACACAGC ATCCTCAAAA 
CGCTGGAAAG AAAAGATAGA CAAAACGAGG AAGGAAATTG AGACAACACA GAATTACCTA 
ATGGATATTA AGAACCGCAT AATACCACCC TTTTTTAAAT CTGAATTGAA CCAGTTATAT 
CAGTCTCAGT ATAATCACCT GGACAGTCTT TCCAAAAAAC TGCCTTCTTT TACAAAGAAA 
AATGAAGATG CAAACGAAAC AGCTGTTCAA GATACATCTG ATCTTAATCT AGAAGCAGAA 
TCTTATGAGC CCGTGAAAAT TCCAACCCAG TCCTTGCTGC AGGATGTTGC GGGACAAACA 
AGAAAAGAAA AAGTGAAGTT GCCTCATTAT TTGCTGAGTT CCAAGCCTAA GTCTCAACCT 
CTTGCAAAGG TGCAAGATTC TGTTGGAGGA AAAGTGAACA CATCCTCTGT TGCATCTGCT 
GCCATTAATA ATGCAAAGTC ATCCCTTTTT GGGTTCCATC TTCTCCCATC ATCAGTCAAG 
TTTGGAGTGC TTAAGGAAGG ACATACCTAT GCCACAGTTG TAAAGCTCAA GAATGTTGGA 
GTGGACTTCT GCAGGTTTAA AGTAAAGCAG CCCCCACCCA GCACAGGACT GAAAGTGACT 
TACAAACCTG GACCTGTGGC AGCTGGTATG CAGACAGAAC TGAATATAGA GTTATTTGCC 
ACAGCTGTTG GAGAGGATGG GGCCAAGGGA TCAGCACACA TCTCTCACAA TATCGAGATT 
ATGACAGAGC ATGAGGTTCT GTTCCTACCT GTGGAAGCAA CTGTTTTAAC AAGCAGCAAT 
TATGATAAAC GACCAAAAGA CTTTCCCCAG GGAAAAGAAA ATCCAATGGT CCAGAGAACT 
TCTACAATTT ATTCCTCCAC ACTTGGAGTC TTCATGTCTC GTAAAGTTTC TCCACATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPAG17 ( XM_011540935.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011539237.1 Homo sapiens sperm associated antigen 17 (SPAG17), transcript variant X5, mRNA.

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CloneID	OHu77635
Clone ID Related Accession (Same CDS sequence)	XM_011540935.2
Accession Version	XM_011540935.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6333bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	sperm-associated antigen 17 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011540935.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATAGTG GAGAGAAATT AACCTTACCA CTGATAGGGA AACTCTTGAA ATTTCAACTT 
CTCCAGATTA AATTTAAGGA CCAACAGCGA CGGGAAAATG AAAAGAAGGT AATAGAAGAC 
AAACCTAAGT TAGAAAAGGA TAAAGGGAAA GCAAAATCTC CCAAGGAGAA AAAGGCTCCA 
AGTGCCAAGC CTGCCAAAGG AAAGGGAAAG GATCAGCCTG AGGCAAATGC ACCAGTGAAA 
AAGACCACCC AGTTAAAGCG GAGAGGAGAA GACGACCACA CCAATCGTTA CATTGACGAT 
GAGCCAGATG ATGGTGCCCA ACATTACATT ATAGTTGTGG GCTTTAACAA TCCTCAGCTA 
TTAGCAATTA TGGCTGAGCT TGGCATTCCT ATAACCAGCG TGATTAAAAT ATCTTCAGAG 
AATTATGAAC CTCTGCAGAC ACACCTGGCA GCAGTTAACC AGCAGCAGGA AGTTCTTCTT 
CAGTCAGAAG ATCTAGAAGC AGAAAAATTG AAGAAAGAAA ATGCCATAAA AGAGCTTAAA 
ACTTTCTGGA AGTACTTGGA ACCAGTCCTG AATAATGAGA AACCTGAAAC AAATCTCTTT 
GATGTTGCTC GACTTGAGTA CATGGTCAAA GCAGCTGATT TTCCTTCTGA CTGGTCAGAT 
GGTGAGATGA TGCTGAAATT GGGCACTGAT ATTTTTGAAA ATATTGCCTG CTTGATGTAT 
GACATCCTGG ATTGGAAAAG GCAGCACCAG CACTATTTGG AAAGCATGCA GCTTATTAAT 
GTTCCACAAG TGGTTAATGA GAAACCTGTA TTAGAAGCCA TGCCAACTTC AGAGGCTCCA 
CAACCTGCTG TACCAGCTCC TGGAAAGAAG AAAGCACAGT ATGAAGAACC GCAAGCTCCA 
CCACCAGTGA CTTCAGTCAT CACAACTGAA GTAGACATGA GATATTACAA TTATTTGCTG 
AATCCAATTC GAGAGGAATT CATTTCTGTG CCCCTGATAC TGCATTGTAT GCTGGAACAG 
GTTGTTGCAA CTGAAGAAGA TCTCGTCCCA CCCAGTCTGC GGGAGCCATC CCCCAGAGCA 
GACGGGCTAG ACCACAGAAT CGCAGCTCAC ATTGTGTCCC TTCTGCCCTC ACTCTGTCTC 
TCAGAGAGGG AGAAAAAGAA TCTTCATGAC ATATTTTTAT CTGAAGAAGA AAATGAAAGC 
AAAGCAGTGC CCAAAGGCCC CCTCCTACTG AACTATCATG ATGCACACGC CCACAAGAAG 
TACGCACTAC AGGACCAAAA GAATTTTGAT CCAGTTCAAA TTGAGCAGGA GATGCAGTCC 
AAGTTGCCAC TGTGGGAATT TCTTCAATTC CCTCTACCCC CACCATGGAA CAACACTAAA 
CGTCTAGCTA CAATTCATGA GCTTATGCAC TTTTGTACGA GTGATGTCTT GAGCTGGAAT 
GAAGTAGAAC GAGCCTTCAA GGTGTTTACT TTTGAGAGCC TGAAGCTCTC TGAGGTTGAT 
GAAAAAGGGA AACTGAAACC TTCTGGGATG ATGTGTGGGT CAGATTCTGA AATGTTCAAC 
ATACCGTGGG ACAACCCTGC CAGATTTGCT AAACAGATAA GGCAGCAATA TGTCATGAAA 
ATGAATACTC AAGAGGCCAA GCAGAAAGCA GATATTAAAA TCAAAGACAG AACACTATTT 
GTGGATCAGA ATTTGTCAAT GTCTGTGCAA GATAATGAAA GCAACCGAGA ACCTTCAGAT 
CCTAGTCAGT GTGATGCTAA CAATATGAAG CATTCTGACT TGAATAATCT CAAACTCTCA 
GTCCCTGATA ATAGACAGCT GTTAGAGCAG GAGAGCATCA TGAAGGCTCA GCCCCAACAT 
GAGTCTCTGG AGCAGACCAC AAACAATGAG ATCAAAGATG ATGCAGTCAC AAAGGCTGAT 
TCTCATGAAA AGAAACCCAA GAAGATGATG GTGGAAGCAG ATTTAGAGGA CATAAAGAAA 
ACACAGCAGC GCAGTCTAAT GGACTGGAGT TTTACTGAAC ATTTTAAACC GAAAGTACTG 
CTTCAGGTCC TTCAAGAAGC CCATAAGCAA TATAGGTGTG TTGATTCTTA CTACCACACC 
CAAGACAACT CTTTACTTTT AGTCTTTCAC AATCCAATGA ATAGACAACG TTTGCATTGT 
GAATATTGGA ACATTGCTCT CCACTCCAAT GTTGGATTCA GGAATTATTT GGAACTTGTT 
GCAAAATCTA TTCAAGATTG GATTACAAAA GAAGAAGCTA TATATCAGGA ATCTAAAATG 
AATGAGAAAA TCATCAGGAC CAGAGCTGAG CTGGAATTGA AATCTTCTGC TAATGCCAAA 
CTTACTTCTG CTAGCAAAAT TTTTTCCATT AAAGAATCTA AAAGTAACAA AGGAATCAGC 
AAAACAGAGA TATCAGATCA AGAAAAAGAA AAAGAGAAGG AAAAGATTCC TTTCATTTTA 
GAAGGCTCTC TCAAGGCATG GAAAGAAGAG CAACATCGAT TAGCAGAAGA GGAGCGCTTA 
AGGGAAGAAA AGAAAGCAGA GAAGAAGGGT AAAGAAGCTG GTAAAAAGAA AGGCAAGGAT 
AACGCAGAGA AAGAGGATAG TAGGTCTTTG AAGAAAAAAT CACCTTACAA GGAGAAATCT 
AAAGAAGAAC AAGTCAAGAT CCAAGAAGTA ACAGAAGAGT CCCCCCACCA ACCAGAACCT 
AAGATAACTT ACCCGTTTCA CGGATACAAT ATGGGAAATA TACCCACTCA AATCTCAGGG 
TCAAATTACT ACCTGTATCC TTCTGATGGG GGGCAGATTG AAGTGGAAAA GACAATGTTT 
GAAAAAGGCC CAACTTTTAT CAAAGTGAGA GTGGTAAAGG ACAACCACAA TTTTATGATT 
CATTTAAATG ACCCTAAGGA AATTGTGAAA AAGGAAGAGA AAGGGGATTA TTATTTAGAA 
GAGGAAGAAG AAGGAGATGA GGAACAAAGT CTTGAAACGG AAGTATCAGA TGCAAAGAAT 
AAAGCTTTCA GCAAGTTTGG ATCTTTTTCT GCCACCTTAG AAAATGGAAT CTGCCTCTCG 
ATAAGTTACT ATGGATCAAA TGGAATGGCA CCAGAAGATA AGGATCCTGA TTTAGAAACA 
ATATTGAATA TCCCTTCAGC ACTCACTCCA ACAGTGGTTC CTGTTATAGT GACCGTTCCT 
CAAAGCAAAG CTAAAGGGAA AATAAAAGGC AAAGAAAAAC CCAAAGAATC CCTTAAAGAA 
GAAGAACACC CAAAAGAAGA AGAGAAAAAG GAAGAAGAAG TAGAACCAGA ACCTGTTTTA 
CAAGAGACTT TGGATGTTCC CACCTTCCAG AGCCTAAATG TGTCTTGCCC CAGTGGGCTC 
CTGTTGACTT TCATTGGACA AGAATCTACA GGTCAATATG TTATAGATGA GGAACCCACC 
TGGGACATCA TGGTCCGTCA GAGCTACCCC CAGAGGGTGA AGCACTATGA GTTCTATAAA 
ACGGTGATGC CACCCGCAGA GCAGGAGGCT TCAAGGGTTA TCACCAGTCA AGGCACTGTT 
GTCAAATATA TGTTGGATGG ATCCACACAG ATTCTCTTTG CAGATGGTGC TGTGAGCAGG 
AGTCCCAATT CAGGTCTTAT TTGTCCTCCT TCTGAAATGC CAGCAACGCC TCACAGTGGA 
GATTTGATGG ACTCTATTTC TCAGCAGAAA TCAGAAACGA TACCATCTGA GATTACCAAC 
ACAAAGAAAG GAAAAAGTCA CAAAAGTCAG TCATCAATGG CCCATAAGGG TGAAATCCAT 
GACCCTCCTC CAGAGGCAGT TCAAACTGTA ACTCCTGTGG AGGTTCACAT AGGCACCTGG 
TTTACAACCA CACCTGAAGG AAATCGGATC GGCACCAAAG GATTAGAAAG AATAGCAGAC 
TTGACCCCAT TGTTATCCTT TCAGGCCACA GATCCTGTCA ATGGAACGGT TATGACAACT 
CGAGAAGACA AAGTTGTCAT AGTTGAAAGG AAAGATGGTA CTCGGATAGT GGATCATGCT 
GATGGTACCA GAATCACAAC CTTTTATCAA GTTTATGAAG ATCAAATTAT TCTGCCAGAT 
GATCAAGAAA CAACCGAGGG TCCTCGGACT GTCACCAGGC AGGTGAAGTG TATGCGGGTA 
GAAAGCTCAC GCTATGCCAC TGTTATCGCC AACTGTGAGG ACAGTAGCTG CTGTGCCACC 
TTTGGAGATG GAACAACTAT TATTGCAAAG CCACAGGGAA CATACCAGGT GTTACCTCCA 
AACACAGGCT CTCTTTATAT TGACAAGGAT TGTTCAGCTG TGTACTGCCA TGAGTCAAGC 
AGTAATATAT ACTATCCTTT TCAAAAGCGT GAGCAGCTGC GAGCTGGCAG GTACATCATG 
AGGCATACTT CAGAGGTTAT CTGTGAGGTT CTGGATCCTG AGGGAAACAC TTTTCAGGTC 
ATGGCTGATG GTAGCATATC AACTATATTA CCTGAAAAAA AATTGGAAGA TGATTTAAAT 
GAGAAAACTG AGGGCTATGA TAGTCTGTCC TCTATGCACC TTGAAAAGAA TCATCAGCAA 
ATCTATGGTG AACATGTCCC CAGGTTTTTT GTTATGTATG CTGATGGATC AGGAATGGAA 
CTTCTTCGAG ACAGTGACAT AGAAGAATAT CTATCTTTGG CATATAAAGA ATCAAATACT 
GTTGTTCTCC AAGAGCCAGT GCAGGAACAG CCAGGCACCC TAACCATCAC AGTCCTTCGC 
CCTTTCCATG AAGCATCACC ATGGCAAGTA AAAAAGGAAG ATACAATTGT CCCTCCTAAT 
CTCCGGTCAA GGTCATGGGA AACATTTCCC TCAGTTGAGA AAAAAACTCC AGGACCTCCG 
TTTGGTACTC AGATTTGGAA AGGCCTTTGC ATTGAGTCCA AACAGCTAGT GAGTGCCCCG 
GGTGCCATAC TCAAGAGCCC CAGTGTGCTA CAGATGCGCC AATTCATTCA GCATGAGGTC 
ATAAAGAATG AGGTGAAACT GAGGCTGCAG GTTTCCCTTA AGGATTACAT AAACTATATT 
CTAAAGAAAG AAGATGAGCT GCAGGAAATG ATGGTTAAAG ATTCCAGAAC TGAGGAGGAG 
AGAGGCAATG CTGCTGATCT CCTCAAGCTG GTTATGTCTT TCCCTAAAAT GGAGGAAACT 
ACAAAAAGTC ATGTTACTGA AGTTGCAGCT CACCTAACTG ATTTATTCAA GCAGTCTTTG 
GCTACGCCTC CAAAATGCCC ACCAGACACA TTTGGTAAAG ATTTCTTTGA AAAGACATGG 
AGACACACAG CATCCTCAAA ACGCTGGAAA GAAAAGATAG ACAAAACGAG GAAGGAAATT 
GAGACAACAC AGAATTACCT AATGGATATT AAGAACCGCA TAATACCACC CTTTTTTAAA 
TCTGAATTGA ACCAGTTATA TCAGTCTCAG TATAATCACC TGGACAGTCT TTCCAAAAAA 
CTGCCTTCTT TTACAAAGAA AAATGAAGAT GCAAACGAAA CAGCTGTTCA AGATACATCT 
GATCTTAATC TAGATTTCAA GCCACATAAG GTTTCAGAAC AGAAATCCTC AAGTGTGCCT 
AGTCTTCCAA AACCAGAGAT TTCTGCAGAT AAGAAGGATT TCACTGCTCA GAACCAAACT 
GAAAATTTAA CAAAATCTCC TGAAGAAGCA GAATCTTATG AGCCCGTGAA AATTCCAACC 
CAGTCCTTGC TGCAGGATGT TGCGGGACAA ACAAGAAAAG AAAAAGTGAA GTTGCCTCAT 
TATTTGCTGA GTTCCAAGCC TAAGTCTCAA CCTCTTGCAA AGGTGCAAGA TTCTGTTGGA 
GGAAAAGTGA ACACATCCTC TGTTGCATCT GCTGCCATTA ATAATGCAAA GTCATCCCTT 
TTTGGGTTCC ATCTTCTCCC ATCATCAGTC AAGTTTGGAG TGCTTAAGGA AGGACATACC 
TATGCCACAG TTGTAAAGCT CAAGAATGTT GGAGTGGACT TCTGCAGGTT TAAAGTAAAG 
CAGCCCCCAC CCAGCACAGG ACTGAAAGTG ACTTACAAAC CTGGACCTGT GGCAGCTGGT 
ATGCAGACAG AACTGAATAT AGAGTTATTT GCCACAGCTG TTGGAGAGGA TGGGGCCAAG 
GGATCAGCAC ACATCTCTCA CAATATCGAG ATTATGACAG AGCATGAGGT TCTGTTCCTA 
CCTGTGGAAG CAACTGTTTT AACAAGCAGC AATTATGATA AACGACCAAA AGACTTTCCC 
CAGGGAAAAG AAAATCCAAT GGTCCAGAGA ACTTCTACAA TTTATTCCTC CACACTTGGA 
GTCTTCATGT CTCGTAAAGT TTCTCCACAT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011539237.1
CDS	247..6579
Translation	MDSGEKLTLPLIGKLLKFQLLQIKFKDQQRRENEKKVIEDKPKLEKDKGKAKSPKEKKAPSAKPAKGKGKDQPEANAPVKKTTQLKRRGEDDHTNRYIDDEPDDGAQHYIIVVGFNNPQLLAIMAELGIPITSVIKISSENYEPLQTHLAAVNQQQEVLLQSEDLEAEKLKKENAIKELKTFWKYLEPVLNNEKPETNLFDVARLEYMVKAADFPSDWSDGEMMLKLGTDIFENIACLMYDILDWKRQHQHYLESMQLINVPQVVNEKPVLEAMPTSEAPQPAVPAPGKKKAQYEEPQAPPPVTSVITTEVDMRYYNYLLNPIREEFISVPLILHCMLEQVVATEEDLVPPSLREPSPRADGLDHRIAAHIVSLLPSLCLSEREKKNLHDIFLSEEENESKAVPKGPLLLNYHDAHAHKKYALQDQKNFDPVQIEQEMQSKLPLWEFLQFPLPPPWNNTKRLATIHELMHFCTSDVLSWNEVERAFKVFTFESLKLSEVDEKGKLKPSGMMCGSDSEMFNIPWDNPARFAKQIRQQYVMKMNTQEAKQKADIKIKDRTLFVDQNLSMSVQDNESNREPSDPSQCDANNMKHSDLNNLKLSVPDNRQLLEQESIMKAQPQHESLEQTTNNEIKDDAVTKADSHEKKPKKMMVEADLEDIKKTQQRSLMDWSFTEHFKPKVLLQVLQEAHKQYRCVDSYYHTQDNSLLLVFHNPMNRQRLHCEYWNIALHSNVGFRNYLELVAKSIQDWITKEEAIYQESKMNEKIIRTRAELELKSSANAKLTSASKIFSIKESKSNKGISKTEISDQEKEKEKEKIPFILEGSLKAWKEEQHRLAEEERLREEKKAEKKGKEAGKKKGKDNAEKEDSRSLKKKSPYKEKSKEEQVKIQEVTEESPHQPEPKITYPFHGYNMGNIPTQISGSNYYLYPSDGGQIEVEKTMFEKGPTFIKVRVVKDNHNFMIHLNDPKEIVKKEEKGDYYLEEEEEGDEEQSLETEVSDAKNKAFSKFGSFSATLENGICLSISYYGSNGMAPEDKDPDLETILNIPSALTPTVVPVIVTVPQSKAKGKIKGKEKPKESLKEEEHPKEEEKKEEEVEPEPVLQETLDVPTFQSLNVSCPSGLLLTFIGQESTGQYVIDEEPTWDIMVRQSYPQRVKHYEFYKTVMPPAEQEASRVITSQGTVVKYMLDGSTQILFADGAVSRSPNSGLICPPSEMPATPHSGDLMDSISQQKSETIPSEITNTKKGKSHKSQSSMAHKGEIHDPPPEAVQTVTPVEVHIGTWFTTTPEGNRIGTKGLERIADLTPLLSFQATDPVNGTVMTTREDKVVIVERKDGTRIVDHADGTRITTFYQVYEDQIILPDDQETTEGPRTVTRQVKCMRVESSRYATVIANCEDSSCCATFGDGTTIIAKPQGTYQVLPPNTGSLYIDKDCSAVYCHESSSNIYYPFQKREQLRAGRYIMRHTSEVICEVLDPEGNTFQVMADGSISTILPEKKLEDDLNEKTEGYDSLSSMHLEKNHQQIYGEHVPRFFVMYADGSGMELLRDSDIEEYLSLAYKESNTVVLQEPVQEQPGTLTITVLRPFHEASPWQVKKEDTIVPPNLRSRSWETFPSVEKKTPGPPFGTQIWKGLCIESKQLVSAPGAILKSPSVLQMRQFIQHEVIKNEVKLRLQVSLKDYINYILKKEDELQEMMVKDSRTEEERGNAADLLKLVMSFPKMEETTKSHVTEVAAHLTDLFKQSLATPPKCPPDTFGKDFFEKTWRHTASSKRWKEKIDKTRKEIETTQNYLMDIKNRIIPPFFKSELNQLYQSQYNHLDSLSKKLPSFTKKNEDANETAVQDTSDLNLDFKPHKVSEQKSSSVPSLPKPEISADKKDFTAQNQTENLTKSPEEAESYEPVKIPTQSLLQDVAGQTRKEKVKLPHYLLSSKPKSQPLAKVQDSVGGKVNTSSVASAAINNAKSSLFGFHLLPSSVKFGVLKEGHTYATVVKLKNVGVDFCRFKVKQPPPSTGLKVTYKPGPVAAGMQTELNIELFATAVGEDGAKGSAHISHNIEIMTEHEVLFLPVEATVLTSSNYDKRPKDFPQGKENPMVQRTSTIYSSTLGVFMSRKVSPH

Target ORF information:

RefSeq Version	XM_011540935.2
Organism	Homo sapiens(human)
Definition	Homo sapiens sperm associated antigen 17 (SPAG17), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011540935.2

ORF Insert Sequence:

ATGGATAGTG GAGAGAAATT AACCTTACCA CTGATAGGGA AACTCTTGAA ATTTCAACTT 
CTCCAGATTA AATTTAAGGA CCAACAGCGA CGGGAAAATG AAAAGAAGGT AATAGAAGAC 
AAACCTAAGT TAGAAAAGGA TAAAGGGAAA GCAAAATCTC CCAAGGAGAA AAAGGCTCCA 
AGTGCCAAGC CTGCCAAAGG AAAGGGAAAG GATCAGCCTG AGGCAAATGC ACCAGTGAAA 
AAGACCACCC AGTTAAAGCG GAGAGGAGAA GACGACCACA CCAATCGTTA CATTGACGAT 
GAGCCAGATG ATGGTGCCCA ACATTACATT ATAGTTGTGG GCTTTAACAA TCCTCAGCTA 
TTAGCAATTA TGGCTGAGCT TGGCATTCCT ATAACCAGCG TGATTAAAAT ATCTTCAGAG 
AATTATGAAC CTCTGCAGAC ACACCTGGCA GCAGTTAACC AGCAGCAGGA AGTTCTTCTT 
CAGTCAGAAG ATCTAGAAGC AGAAAAATTG AAGAAAGAAA ATGCCATAAA AGAGCTTAAA 
ACTTTCTGGA AGTACTTGGA ACCAGTCCTG AATAATGAGA AACCTGAAAC AAATCTCTTT 
GATGTTGCTC GACTTGAGTA CATGGTCAAA GCAGCTGATT TTCCTTCTGA CTGGTCAGAT 
GGTGAGATGA TGCTGAAATT GGGCACTGAT ATTTTTGAAA ATATTGCCTG CTTGATGTAT 
GACATCCTGG ATTGGAAAAG GCAGCACCAG CACTATTTGG AAAGCATGCA GCTTATTAAT 
GTTCCACAAG TGGTTAATGA GAAACCTGTA TTAGAAGCCA TGCCAACTTC AGAGGCTCCA 
CAACCTGCTG TACCAGCTCC TGGAAAGAAG AAAGCACAGT ATGAAGAACC GCAAGCTCCA 
CCACCAGTGA CTTCAGTCAT CACAACTGAA GTAGACATGA GATATTACAA TTATTTGCTG 
AATCCAATTC GAGAGGAATT CATTTCTGTG CCCCTGATAC TGCATTGTAT GCTGGAACAG 
GTTGTTGCAA CTGAAGAAGA TCTCGTCCCA CCCAGTCTGC GGGAGCCATC CCCCAGAGCA 
GACGGGCTAG ACCACAGAAT CGCAGCTCAC ATTGTGTCCC TTCTGCCCTC ACTCTGTCTC 
TCAGAGAGGG AGAAAAAGAA TCTTCATGAC ATATTTTTAT CTGAAGAAGA AAATGAAAGC 
AAAGCAGTGC CCAAAGGCCC CCTCCTACTG AACTATCATG ATGCACACGC CCACAAGAAG 
TACGCACTAC AGGACCAAAA GAATTTTGAT CCAGTTCAAA TTGAGCAGGA GATGCAGTCC 
AAGTTGCCAC TGTGGGAATT TCTTCAATTC CCTCTACCCC CACCATGGAA CAACACTAAA 
CGTCTAGCTA CAATTCATGA GCTTATGCAC TTTTGTACGA GTGATGTCTT GAGCTGGAAT 
GAAGTAGAAC GAGCCTTCAA GGTGTTTACT TTTGAGAGCC TGAAGCTCTC TGAGGTTGAT 
GAAAAAGGGA AACTGAAACC TTCTGGGATG ATGTGTGGGT CAGATTCTGA AATGTTCAAC 
ATACCGTGGG ACAACCCTGC CAGATTTGCT AAACAGATAA GGCAGCAATA TGTCATGAAA 
ATGAATACTC AAGAGGCCAA GCAGAAAGCA GATATTAAAA TCAAAGACAG AACACTATTT 
GTGGATCAGA ATTTGTCAAT GTCTGTGCAA GATAATGAAA GCAACCGAGA ACCTTCAGAT 
CCTAGTCAGT GTGATGCTAA CAATATGAAG CATTCTGACT TGAATAATCT CAAACTCTCA 
GTCCCTGATA ATAGACAGCT GTTAGAGCAG GAGAGCATCA TGAAGGCTCA GCCCCAACAT 
GAGTCTCTGG AGCAGACCAC AAACAATGAG ATCAAAGATG ATGCAGTCAC AAAGGCTGAT 
TCTCATGAAA AGAAACCCAA GAAGATGATG GTGGAAGCAG ATTTAGAGGA CATAAAGAAA 
ACACAGCAGC GCAGTCTAAT GGACTGGAGT TTTACTGAAC ATTTTAAACC GAAAGTACTG 
CTTCAGGTCC TTCAAGAAGC CCATAAGCAA TATAGGTGTG TTGATTCTTA CTACCACACC 
CAAGACAACT CTTTACTTTT AGTCTTTCAC AATCCAATGA ATAGACAACG TTTGCATTGT 
GAATATTGGA ACATTGCTCT CCACTCCAAT GTTGGATTCA GGAATTATTT GGAACTTGTT 
GCAAAATCTA TTCAAGATTG GATTACAAAA GAAGAAGCTA TATATCAGGA ATCTAAAATG 
AATGAGAAAA TCATCAGGAC CAGAGCTGAG CTGGAATTGA AATCTTCTGC TAATGCCAAA 
CTTACTTCTG CTAGCAAAAT TTTTTCCATT AAAGAATCTA AAAGTAACAA AGGAATCAGC 
AAAACAGAGA TATCAGATCA AGAAAAAGAA AAAGAGAAGG AAAAGATTCC TTTCATTTTA 
GAAGGCTCTC TCAAGGCATG GAAAGAAGAG CAACATCGAT TAGCAGAAGA GGAGCGCTTA 
AGGGAAGAAA AGAAAGCAGA GAAGAAGGGT AAAGAAGCTG GTAAAAAGAA AGGCAAGGAT 
AACGCAGAGA AAGAGGATAG TAGGTCTTTG AAGAAAAAAT CACCTTACAA GGAGAAATCT 
AAAGAAGAAC AAGTCAAGAT CCAAGAAGTA ACAGAAGAGT CCCCCCACCA ACCAGAACCT 
AAGATAACTT ACCCGTTTCA CGGATACAAT ATGGGAAATA TACCCACTCA AATCTCAGGG 
TCAAATTACT ACCTGTATCC TTCTGATGGG GGGCAGATTG AAGTGGAAAA GACAATGTTT 
GAAAAAGGCC CAACTTTTAT CAAAGTGAGA GTGGTAAAGG ACAACCACAA TTTTATGATT 
CATTTAAATG ACCCTAAGGA AATTGTGAAA AAGGAAGAGA AAGGGGATTA TTATTTAGAA 
GAGGAAGAAG AAGGAGATGA GGAACAAAGT CTTGAAACGG AAGTATCAGA TGCAAAGAAT 
AAAGCTTTCA GCAAGTTTGG ATCTTTTTCT GCCACCTTAG AAAATGGAAT CTGCCTCTCG 
ATAAGTTACT ATGGATCAAA TGGAATGGCA CCAGAAGATA AGGATCCTGA TTTAGAAACA 
ATATTGAATA TCCCTTCAGC ACTCACTCCA ACAGTGGTTC CTGTTATAGT GACCGTTCCT 
CAAAGCAAAG CTAAAGGGAA AATAAAAGGC AAAGAAAAAC CCAAAGAATC CCTTAAAGAA 
GAAGAACACC CAAAAGAAGA AGAGAAAAAG GAAGAAGAAG TAGAACCAGA ACCTGTTTTA 
CAAGAGACTT TGGATGTTCC CACCTTCCAG AGCCTAAATG TGTCTTGCCC CAGTGGGCTC 
CTGTTGACTT TCATTGGACA AGAATCTACA GGTCAATATG TTATAGATGA GGAACCCACC 
TGGGACATCA TGGTCCGTCA GAGCTACCCC CAGAGGGTGA AGCACTATGA GTTCTATAAA 
ACGGTGATGC CACCCGCAGA GCAGGAGGCT TCAAGGGTTA TCACCAGTCA AGGCACTGTT 
GTCAAATATA TGTTGGATGG ATCCACACAG ATTCTCTTTG CAGATGGTGC TGTGAGCAGG 
AGTCCCAATT CAGGTCTTAT TTGTCCTCCT TCTGAAATGC CAGCAACGCC TCACAGTGGA 
GATTTGATGG ACTCTATTTC TCAGCAGAAA TCAGAAACGA TACCATCTGA GATTACCAAC 
ACAAAGAAAG GAAAAAGTCA CAAAAGTCAG TCATCAATGG CCCATAAGGG TGAAATCCAT 
GACCCTCCTC CAGAGGCAGT TCAAACTGTA ACTCCTGTGG AGGTTCACAT AGGCACCTGG 
TTTACAACCA CACCTGAAGG AAATCGGATC GGCACCAAAG GATTAGAAAG AATAGCAGAC 
TTGACCCCAT TGTTATCCTT TCAGGCCACA GATCCTGTCA ATGGAACGGT TATGACAACT 
CGAGAAGACA AAGTTGTCAT AGTTGAAAGG AAAGATGGTA CTCGGATAGT GGATCATGCT 
GATGGTACCA GAATCACAAC CTTTTATCAA GTTTATGAAG ATCAAATTAT TCTGCCAGAT 
GATCAAGAAA CAACCGAGGG TCCTCGGACT GTCACCAGGC AGGTGAAGTG TATGCGGGTA 
GAAAGCTCAC GCTATGCCAC TGTTATCGCC AACTGTGAGG ACAGTAGCTG CTGTGCCACC 
TTTGGAGATG GAACAACTAT TATTGCAAAG CCACAGGGAA CATACCAGGT GTTACCTCCA 
AACACAGGCT CTCTTTATAT TGACAAGGAT TGTTCAGCTG TGTACTGCCA TGAGTCAAGC 
AGTAATATAT ACTATCCTTT TCAAAAGCGT GAGCAGCTGC GAGCTGGCAG GTACATCATG 
AGGCATACTT CAGAGGTTAT CTGTGAGGTT CTGGATCCTG AGGGAAACAC TTTTCAGGTC 
ATGGCTGATG GTAGCATATC AACTATATTA CCTGAAAAAA AATTGGAAGA TGATTTAAAT 
GAGAAAACTG AGGGCTATGA TAGTCTGTCC TCTATGCACC TTGAAAAGAA TCATCAGCAA 
ATCTATGGTG AACATGTCCC CAGGTTTTTT GTTATGTATG CTGATGGATC AGGAATGGAA 
CTTCTTCGAG ACAGTGACAT AGAAGAATAT CTATCTTTGG CATATAAAGA ATCAAATACT 
GTTGTTCTCC AAGAGCCAGT GCAGGAACAG CCAGGCACCC TAACCATCAC AGTCCTTCGC 
CCTTTCCATG AAGCATCACC ATGGCAAGTA AAAAAGGAAG ATACAATTGT CCCTCCTAAT 
CTCCGGTCAA GGTCATGGGA AACATTTCCC TCAGTTGAGA AAAAAACTCC AGGACCTCCG 
TTTGGTACTC AGATTTGGAA AGGCCTTTGC ATTGAGTCCA AACAGCTAGT GAGTGCCCCG 
GGTGCCATAC TCAAGAGCCC CAGTGTGCTA CAGATGCGCC AATTCATTCA GCATGAGGTC 
ATAAAGAATG AGGTGAAACT GAGGCTGCAG GTTTCCCTTA AGGATTACAT AAACTATATT 
CTAAAGAAAG AAGATGAGCT GCAGGAAATG ATGGTTAAAG ATTCCAGAAC TGAGGAGGAG 
AGAGGCAATG CTGCTGATCT CCTCAAGCTG GTTATGTCTT TCCCTAAAAT GGAGGAAACT 
ACAAAAAGTC ATGTTACTGA AGTTGCAGCT CACCTAACTG ATTTATTCAA GCAGTCTTTG 
GCTACGCCTC CAAAATGCCC ACCAGACACA TTTGGTAAAG ATTTCTTTGA AAAGACATGG 
AGACACACAG CATCCTCAAA ACGCTGGAAA GAAAAGATAG ACAAAACGAG GAAGGAAATT 
GAGACAACAC AGAATTACCT AATGGATATT AAGAACCGCA TAATACCACC CTTTTTTAAA 
TCTGAATTGA ACCAGTTATA TCAGTCTCAG TATAATCACC TGGACAGTCT TTCCAAAAAA 
CTGCCTTCTT TTACAAAGAA AAATGAAGAT GCAAACGAAA CAGCTGTTCA AGATACATCT 
GATCTTAATC TAGATTTCAA GCCACATAAG GTTTCAGAAC AGAAATCCTC AAGTGTGCCT 
AGTCTTCCAA AACCAGAGAT TTCTGCAGAT AAGAAGGATT TCACTGCTCA GAACCAAACT 
GAAAATTTAA CAAAATCTCC TGAAGAAGCA GAATCTTATG AGCCCGTGAA AATTCCAACC 
CAGTCCTTGC TGCAGGATGT TGCGGGACAA ACAAGAAAAG AAAAAGTGAA GTTGCCTCAT 
TATTTGCTGA GTTCCAAGCC TAAGTCTCAA CCTCTTGCAA AGGTGCAAGA TTCTGTTGGA 
GGAAAAGTGA ACACATCCTC TGTTGCATCT GCTGCCATTA ATAATGCAAA GTCATCCCTT 
TTTGGGTTCC ATCTTCTCCC ATCATCAGTC AAGTTTGGAG TGCTTAAGGA AGGACATACC 
TATGCCACAG TTGTAAAGCT CAAGAATGTT GGAGTGGACT TCTGCAGGTT TAAAGTAAAG 
CAGCCCCCAC CCAGCACAGG ACTGAAAGTG ACTTACAAAC CTGGACCTGT GGCAGCTGGT 
ATGCAGACAG AACTGAATAT AGAGTTATTT GCCACAGCTG TTGGAGAGGA TGGGGCCAAG 
GGATCAGCAC ACATCTCTCA CAATATCGAG ATTATGACAG AGCATGAGGT TCTGTTCCTA 
CCTGTGGAAG CAACTGTTTT AACAAGCAGC AATTATGATA AACGACCAAA AGACTTTCCC 
CAGGGAAAAG AAAATCCAAT GGTCCAGAGA ACTTCTACAA TTTATTCCTC CACACTTGGA 
GTCTTCATGT CTCGTAAAGT TTCTCCACAT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	SPAG17
Entrez Gene ID	200162
Full Name	sperm associated antigen 17
Synonyms	CT143,PF6
General protein information	Preferred Names sperm associated antigen 17 Names sperm-associated antigen 17 projection protein PF6 homolog
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 1 Map Location: 1p12
Summary	This gene encodes a central pair protein present in the axonemes of cells with a '9 + 2' organization of microtubules. The encoded protein is required for the proper function of the axoneme. Mutations in the orthologous gene in mice lead to primary ciliary dyskinesia characterized by immotile nasal and tracheal cilia, reduced clearance of nasal mucus, profound respiratory distress, hydrocephalus, and neonatal lethality within twelve hours of birth due to impaired airway mucociliary clearance. Single-nucleotide polymorphisms in this gene are associated with human height and targeted mutations lead to skeletal malformations affecting the limbs in mice, suggesting a role for this gene in skeletal development. [provided by RefSeq, Feb 2017]. COMPLETENESS: complete on the 3' end.
Disorder MIM:	616554

Xenopus tropicalis (tropical clawed frog)	LOC100170502	NP_001123754.1
Homo sapiens (human)	SPAG17	NP_996879.1
Pan troglodytes (chimpanzee)	SPAG17	XP_513687.2
	SPAG17	XP_002801770.1
Canis lupus familiaris (dog)	SPAG17	XP_849615.1
Mus musculus (house mouse)	Spag17	NP_083168.3
Gallus gallus (chicken)	SPAG17	XP_416538.4
Bos taurus (cattle)	SPAG17	NP_001193803.1
Rattus norvegicus (Norway rat)	Spag17	XP_006233092.1
Danio rerio (zebrafish)	si:dkey-208b23.5	XP_689610.5

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

SPAG17 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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