ELANE cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	ELANE
Entrez Gene ID	1991
Full Name	elastase, neutrophil expressed
Synonyms	ELA2,GE,HLE,HNE,NE,PMN-E,SCN1
General protein information	Preferred Names elastase, neutrophil expressed Names neutrophil elastase PMN elastase bone marrow serine protease elastase 2, neutrophil elastase-2 granulocyte-derived elastase leukocyte elastase medullasin polymorphonuclear elastase
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 19 Map Location: 19p13.3
Summary	Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode structurally similar proteins. The encoded preproprotein is proteolytically processed to generate the active protease. Following activation, this protease hydrolyzes proteins within specialized neutrophil lysosomes, called azurophil granules, as well as proteins of the extracellular matrix. The enzyme may play a role in degenerative and inflammatory diseases through proteolysis of collagen-IV and elastin. This protein also degrades the outer membrane protein A (OmpA) of E. coli as well as the virulence factors of such bacteria as Shigella, Salmonella and Yersinia. Mutations in this gene are associated with cyclic neutropenia and severe congenital neutropenia (SCN). This gene is present in a gene cluster on chromosome 19. [provided by RefSeq, Jan 2016].
Disorder MIM:	130130
Disorder Html:	Neutropenia, cyclic, 162800 (3); Neutropenia, severe congenital 1, autosomal dominant, 202700 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001972.2	NP_001963.1	neutrophil elastase preproprotein
NM_001972.3	NP_001963.1	neutrophil elastase preproprotein
NM_001972.4	NP_001963.1	neutrophil elastase preproprotein
XM_011527775.1	XP_011526077.1	neutrophil elastase isoform X1
XM_011546888.1	XP_011545190.1	neutrophil elastase isoform X1
XM_011527776.1	XP_011526078.1	neutrophil elastase isoform X1

Pathways from BioSystems

Homologies

Bos taurus (cattle)	ELANE	NP_001099123.1
Mus musculus (house mouse)	Elane	NP_056594.2
Homo sapiens (human)	ELANE	NP_001963.1
Canis lupus familiaris (dog)	ELANE	NP_001003378.1
Pan troglodytes (chimpanzee)	ELANE	XP_001172130.3
Rattus norvegicus (Norway rat)	Elane	NP_001100237.1

Gene Ontology
Bibliography

Related articles in PubMed

Elastase inhibitors as potential therapies for
Makaryan V, Kelley ML, Fletcher B, Bolyard AA, Aprikyan AA, Dale DC
Journal of leukocyte biology102(4)1143-1151(2017 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Coincubation with the inhibitors, particularly MK0339, promoted cell survival and increased formation of mature neutrophils. These studies suggest that cell-permeable inhibitors of neutrophil elastase show promise as novel therapies for ELANE-associated neutropenia.
Title: Elastase inhibitors as potential therapies for

The role of neutrophil elastase in the activation of unfolded protein response effector molecules via PERK and CHOP is reported.
Title: Human neutrophil elastase induces endothelial cell apoptosis by activating the PERK-CHOP branch of the unfolded protein response.

Taken together, these observations support that the unusual HNE N-glycosylation, here reported for the first time, is involved in modulating multiple immune functions central to inflammation and infection.
Title: Paucimannose-Rich

Data suggest that neuropilin-1 (NRP1) functions as a receptor for neutrophil elastase (NE), mediates uptake/absorption of NE, and facilitates cross-presentation of PR1 (a peptide fragment of NE) in breast cancer cells.
Title: Neuropilin-1 mediates neutrophil elastase uptake and cross-presentation in breast cancer cells.

This study proposes that acquisition of CSF3R mutations may represent a mechanism by which myeloid precursor cells carrying the ELANE mutations evade the proapoptotic activity of the Neutrophil Elastase mutants in SCN patients.
Title: A Truncated Granulocyte Colony-stimulating Factor Receptor (G-CSFR) Inhibits Apoptosis Induced by Neutrophil Elastase G185R Mutant: IMPLICATION FOR UNDERSTANDING CSF3R GENE MUTATIONS IN SEVERE CONGENITAL NEUTROPENIA.

The following ELANE gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ELANE cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu19461	NM_001972.4 Latest version!	Homo sapiens elastase, neutrophil expressed (ELANE), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$99.50-$139.30 $199.00
OHu19461	XM_011527776.1 Latest version!	Homo sapiens elastase, neutrophil expressed (ELANE), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$99.50-$139.30 $199.00
OHu19461	XM_011527775.1 Latest version!	Homo sapiens elastase, neutrophil expressed (ELANE), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$99.50-$139.30 $199.00
OHu19461	XM_011546888.1 Latest version!	Homo sapiens elastase, neutrophil expressed (ELANE), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$99.50-$139.30 $199.00
View Old Accession Versions >>
OHu19461	NM_001972.2	Homo sapiens elastase, neutrophil expressed (ELANE), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$99.50-$139.30 $199.00
OHu19461	NM_001972.3	Homo sapiens elastase, neutrophil expressed (ELANE), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$99.50-$139.30 $199.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ELANE ( NM_001972.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001963.1 Homo sapiens elastase, neutrophil expressed (ELANE), mRNA.

Price & Availability↑

CloneID	OHu19461
Clone ID Related Accession (Same CDS sequence)	NM_001972.2 , NM_001972.3 , NM_001972.4 , XM_011527775.1 , XM_011546888.1 , XM_011527776.1
Accession Version	NM_001972.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 804bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-14
Organism	Homo sapiens(Human)
Product	neutrophil elastase preproprotein
Comment

ATGACCCTCG GCCGCCGACT CGCGTGTCTT TTCCTCGCCT GTGTCCTGCC GGCCTTGCTG 
CTGGGGGGCA CCGCGCTGGC CTCGGAGATT GTGGGGGGCC GGCGAGCGCG GCCCCACGCG 
TGGCCCTTCA TGGTGTCCCT GCAGCTGCGC GGAGGCCACT TCTGCGGCGC CACCCTGATT 
GCGCCCAACT TCGTCATGTC GGCCGCGCAC TGCGTGGCGA ATGTAAACGT CCGCGCGGTG 
CGGGTGGTCC TGGGAGCCCA TAACCTCTCG CGGCGGGAGC CCACCCGGCA GGTGTTCGCC 
GTGCAGCGCA TCTTCGAAAA CGGCTACGAC CCCGTAAACT TGCTCAACGA CATCGTGATT 
CTCCAGCTCA ACGGGTCGGC CACCATCAAC GCCAACGTGC AGGTGGCCCA GCTGCCGGCT 
CAGGGACGCC GCCTGGGCAA CGGGGTGCAG TGCCTGGCCA TGGGCTGGGG CCTTCTGGGC 
AGGAACCGTG GGATCGCCAG CGTCCTGCAG GAGCTCAACG TGACGGTGGT GACGTCCCTC 
TGCCGTCGCA GCAACGTCTG CACTCTCGTG AGGGGCCGGC AGGCCGGCGT CTGTTTCGGG 
GACTCCGGCA GCCCCTTGGT CTGCAACGGG CTAATCCACG GAATTGCCTC CTTCGTCCGG 
GGAGGCTGCG CCTCAGGGCT CTACCCCGAT GCCTTTGCCC CGGTGGCACA GTTTGTAAAC 
TGGATCGACT CTATCATCCA ACGCTCCGAG GACAACCCCT GTCCCCACCC CCGGGACCCG 
GACCCGGCCA GCAGGACCCA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001963.1
Translation	MTLGRRLACLFLACVLPALLLGGTALASEIVGGRRARPHAWPFMVSLQLRGGHFCGATLIAPNFVMSAAHCVANVNVRAVRVVLGAHNLSRREPTRQVFAVQRIFENGYDPVNLLNDIVILQLNGSATINANVQVAQLPAQGRRLGNGVQCLAMGWGLLGRNRGIASVLQELNVTVVTSLCRRSNVCTLVRGRQAGVCFGDSGSPLVCNGLIHGIASFVRGGCASGLYPDAFAPVAQFVNWIDSIIQRSEDNPCPHPRDPDPASRTH

Target ORF information:

RefSeq Version	NM_001972.2
Organism	Homo sapiens(Human)
Definition	Homo sapiens elastase, neutrophil expressed (ELANE), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001972.2

ORF Insert Sequence:

ATGACCCTCG GCCGCCGACT CGCGTGTCTT TTCCTCGCCT GTGTCCTGCC GGCCTTGCTG 
CTGGGGGGCA CCGCGCTGGC CTCGGAGATT GTGGGGGGCC GGCGAGCGCG GCCCCACGCG 
TGGCCCTTCA TGGTGTCCCT GCAGCTGCGC GGAGGCCACT TCTGCGGCGC CACCCTGATT 
GCGCCCAACT TCGTCATGTC GGCCGCGCAC TGCGTGGCGA ATGTAAACGT CCGCGCGGTG 
CGGGTGGTCC TGGGAGCCCA TAACCTCTCG CGGCGGGAGC CCACCCGGCA GGTGTTCGCC 
GTGCAGCGCA TCTTCGAAAA CGGCTACGAC CCCGTAAACT TGCTCAACGA CATCGTGATT 
CTCCAGCTCA ACGGGTCGGC CACCATCAAC GCCAACGTGC AGGTGGCCCA GCTGCCGGCT 
CAGGGACGCC GCCTGGGCAA CGGGGTGCAG TGCCTGGCCA TGGGCTGGGG CCTTCTGGGC 
AGGAACCGTG GGATCGCCAG CGTCCTGCAG GAGCTCAACG TGACGGTGGT GACGTCCCTC 
TGCCGTCGCA GCAACGTCTG CACTCTCGTG AGGGGCCGGC AGGCCGGCGT CTGTTTCGGG 
GACTCCGGCA GCCCCTTGGT CTGCAACGGG CTAATCCACG GAATTGCCTC CTTCGTCCGG 
GGAGGCTGCG CCTCAGGGCT CTACCCCGAT GCCTTTGCCC CGGTGGCACA GTTTGTAAAC 
TGGATCGACT CTATCATCCA ACGCTCCGAG GACAACCCCT GTCCCCACCC CCGGGACCCG 
GACCCGGCCA GCAGGACCCA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ELANE ( NM_001972.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001963.1 Homo sapiens elastase, neutrophil expressed (ELANE), mRNA.

Price & Availability↑

CloneID	OHu19461
Clone ID Related Accession (Same CDS sequence)	NM_001972.2 , NM_001972.3 , NM_001972.4 , XM_011527775.1 , XM_011546888.1 , XM_011527776.1
Accession Version	NM_001972.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 804bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-27
Organism	Homo sapiens(human)
Product	neutrophil elastase preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CD613590.1, M34379.1 and BI254663.1. On Jan 8, 2016 this sequence version replaced NM_001972.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: M34379.1, X05875.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2142348 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACCCTCG GCCGCCGACT CGCGTGTCTT TTCCTCGCCT GTGTCCTGCC GGCCTTGCTG 
CTGGGGGGCA CCGCGCTGGC CTCGGAGATT GTGGGGGGCC GGCGAGCGCG GCCCCACGCG 
TGGCCCTTCA TGGTGTCCCT GCAGCTGCGC GGAGGCCACT TCTGCGGCGC CACCCTGATT 
GCGCCCAACT TCGTCATGTC GGCCGCGCAC TGCGTGGCGA ATGTAAACGT CCGCGCGGTG 
CGGGTGGTCC TGGGAGCCCA TAACCTCTCG CGGCGGGAGC CCACCCGGCA GGTGTTCGCC 
GTGCAGCGCA TCTTCGAAAA CGGCTACGAC CCCGTAAACT TGCTCAACGA CATCGTGATT 
CTCCAGCTCA ACGGGTCGGC CACCATCAAC GCCAACGTGC AGGTGGCCCA GCTGCCGGCT 
CAGGGACGCC GCCTGGGCAA CGGGGTGCAG TGCCTGGCCA TGGGCTGGGG CCTTCTGGGC 
AGGAACCGTG GGATCGCCAG CGTCCTGCAG GAGCTCAACG TGACGGTGGT GACGTCCCTC 
TGCCGTCGCA GCAACGTCTG CACTCTCGTG AGGGGCCGGC AGGCCGGCGT CTGTTTCGGG 
GACTCCGGCA GCCCCTTGGT CTGCAACGGG CTAATCCACG GAATTGCCTC CTTCGTCCGG 
GGAGGCTGCG CCTCAGGGCT CTACCCCGAT GCCTTTGCCC CGGTGGCACA GTTTGTAAAC 
TGGATCGACT CTATCATCCA ACGCTCCGAG GACAACCCCT GTCCCCACCC CCGGGACCCG 
GACCCGGCCA GCAGGACCCA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001963.1
CDS	121..924
Translation	MTLGRRLACLFLACVLPALLLGGTALASEIVGGRRARPHAWPFMVSLQLRGGHFCGATLIAPNFVMSAAHCVANVNVRAVRVVLGAHNLSRREPTRQVFAVQRIFENGYDPVNLLNDIVILQLNGSATINANVQVAQLPAQGRRLGNGVQCLAMGWGLLGRNRGIASVLQELNVTVVTSLCRRSNVCTLVRGRQAGVCFGDSGSPLVCNGLIHGIASFVRGGCASGLYPDAFAPVAQFVNWIDSIIQRSEDNPCPHPRDPDPASRTH

Target ORF information:

RefSeq Version	NM_001972.3
Organism	Homo sapiens(human)
Definition	Homo sapiens elastase, neutrophil expressed (ELANE), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001972.3

ORF Insert Sequence:

ATGACCCTCG GCCGCCGACT CGCGTGTCTT TTCCTCGCCT GTGTCCTGCC GGCCTTGCTG 
CTGGGGGGCA CCGCGCTGGC CTCGGAGATT GTGGGGGGCC GGCGAGCGCG GCCCCACGCG 
TGGCCCTTCA TGGTGTCCCT GCAGCTGCGC GGAGGCCACT TCTGCGGCGC CACCCTGATT 
GCGCCCAACT TCGTCATGTC GGCCGCGCAC TGCGTGGCGA ATGTAAACGT CCGCGCGGTG 
CGGGTGGTCC TGGGAGCCCA TAACCTCTCG CGGCGGGAGC CCACCCGGCA GGTGTTCGCC 
GTGCAGCGCA TCTTCGAAAA CGGCTACGAC CCCGTAAACT TGCTCAACGA CATCGTGATT 
CTCCAGCTCA ACGGGTCGGC CACCATCAAC GCCAACGTGC AGGTGGCCCA GCTGCCGGCT 
CAGGGACGCC GCCTGGGCAA CGGGGTGCAG TGCCTGGCCA TGGGCTGGGG CCTTCTGGGC 
AGGAACCGTG GGATCGCCAG CGTCCTGCAG GAGCTCAACG TGACGGTGGT GACGTCCCTC 
TGCCGTCGCA GCAACGTCTG CACTCTCGTG AGGGGCCGGC AGGCCGGCGT CTGTTTCGGG 
GACTCCGGCA GCCCCTTGGT CTGCAACGGG CTAATCCACG GAATTGCCTC CTTCGTCCGG 
GGAGGCTGCG CCTCAGGGCT CTACCCCGAT GCCTTTGCCC CGGTGGCACA GTTTGTAAAC 
TGGATCGACT CTATCATCCA ACGCTCCGAG GACAACCCCT GTCCCCACCC CCGGGACCCG 
GACCCGGCCA GCAGGACCCA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ELANE ( NM_001972.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_001963.1 Homo sapiens elastase, neutrophil expressed (ELANE), mRNA.

Price & Availability↑

CloneID	OHu19461
Clone ID Related Accession (Same CDS sequence)	NM_001972.2 , NM_001972.3 , NM_001972.4 , XM_011527775.1 , XM_011546888.1 , XM_011527776.1
Accession Version	NM_001972.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 804bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-03
Organism	Homo sapiens(human)
Product	neutrophil elastase preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from M34379.1 and BI254663.1. On Nov 22, 2018 this sequence version replaced NM_001972.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: M34379.1, X05875.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2142348 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000263621.2/ ENSP00000263621.1 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGACCCTCG GCCGCCGACT CGCGTGTCTT TTCCTCGCCT GTGTCCTGCC GGCCTTGCTG 
CTGGGGGGCA CCGCGCTGGC CTCGGAGATT GTGGGGGGCC GGCGAGCGCG GCCCCACGCG 
TGGCCCTTCA TGGTGTCCCT GCAGCTGCGC GGAGGCCACT TCTGCGGCGC CACCCTGATT 
GCGCCCAACT TCGTCATGTC GGCCGCGCAC TGCGTGGCGA ATGTAAACGT CCGCGCGGTG 
CGGGTGGTCC TGGGAGCCCA TAACCTCTCG CGGCGGGAGC CCACCCGGCA GGTGTTCGCC 
GTGCAGCGCA TCTTCGAAAA CGGCTACGAC CCCGTAAACT TGCTCAACGA CATCGTGATT 
CTCCAGCTCA ACGGGTCGGC CACCATCAAC GCCAACGTGC AGGTGGCCCA GCTGCCGGCT 
CAGGGACGCC GCCTGGGCAA CGGGGTGCAG TGCCTGGCCA TGGGCTGGGG CCTTCTGGGC 
AGGAACCGTG GGATCGCCAG CGTCCTGCAG GAGCTCAACG TGACGGTGGT GACGTCCCTC 
TGCCGTCGCA GCAACGTCTG CACTCTCGTG AGGGGCCGGC AGGCCGGCGT CTGTTTCGGG 
GACTCCGGCA GCCCCTTGGT CTGCAACGGG CTAATCCACG GAATTGCCTC CTTCGTCCGG 
GGAGGCTGCG CCTCAGGGCT CTACCCCGAT GCCTTTGCCC CGGTGGCACA GTTTGTAAAC 
TGGATCGACT CTATCATCCA ACGCTCCGAG GACAACCCCT GTCCCCACCC CCGGGACCCG 
GACCCGGCCA GCAGGACCCA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001963.1
CDS	27..830
Translation	MTLGRRLACLFLACVLPALLLGGTALASEIVGGRRARPHAWPFMVSLQLRGGHFCGATLIAPNFVMSAAHCVANVNVRAVRVVLGAHNLSRREPTRQVFAVQRIFENGYDPVNLLNDIVILQLNGSATINANVQVAQLPAQGRRLGNGVQCLAMGWGLLGRNRGIASVLQELNVTVVTSLCRRSNVCTLVRGRQAGVCFGDSGSPLVCNGLIHGIASFVRGGCASGLYPDAFAPVAQFVNWIDSIIQRSEDNPCPHPRDPDPASRTH

Target ORF information:

RefSeq Version	NM_001972.4
Organism	Homo sapiens(human)
Definition	Homo sapiens elastase, neutrophil expressed (ELANE), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001972.4

ORF Insert Sequence:

ATGACCCTCG GCCGCCGACT CGCGTGTCTT TTCCTCGCCT GTGTCCTGCC GGCCTTGCTG 
CTGGGGGGCA CCGCGCTGGC CTCGGAGATT GTGGGGGGCC GGCGAGCGCG GCCCCACGCG 
TGGCCCTTCA TGGTGTCCCT GCAGCTGCGC GGAGGCCACT TCTGCGGCGC CACCCTGATT 
GCGCCCAACT TCGTCATGTC GGCCGCGCAC TGCGTGGCGA ATGTAAACGT CCGCGCGGTG 
CGGGTGGTCC TGGGAGCCCA TAACCTCTCG CGGCGGGAGC CCACCCGGCA GGTGTTCGCC 
GTGCAGCGCA TCTTCGAAAA CGGCTACGAC CCCGTAAACT TGCTCAACGA CATCGTGATT 
CTCCAGCTCA ACGGGTCGGC CACCATCAAC GCCAACGTGC AGGTGGCCCA GCTGCCGGCT 
CAGGGACGCC GCCTGGGCAA CGGGGTGCAG TGCCTGGCCA TGGGCTGGGG CCTTCTGGGC 
AGGAACCGTG GGATCGCCAG CGTCCTGCAG GAGCTCAACG TGACGGTGGT GACGTCCCTC 
TGCCGTCGCA GCAACGTCTG CACTCTCGTG AGGGGCCGGC AGGCCGGCGT CTGTTTCGGG 
GACTCCGGCA GCCCCTTGGT CTGCAACGGG CTAATCCACG GAATTGCCTC CTTCGTCCGG 
GGAGGCTGCG CCTCAGGGCT CTACCCCGAT GCCTTTGCCC CGGTGGCACA GTTTGTAAAC 
TGGATCGACT CTATCATCCA ACGCTCCGAG GACAACCCCT GTCCCCACCC CCGGGACCCG 
GACCCGGCCA GCAGGACCCA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ELANE ( XM_011527775.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011526077.1 Homo sapiens elastase, neutrophil expressed (ELANE), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu19461
Clone ID Related Accession (Same CDS sequence)	NM_001972.2 , NM_001972.3 , NM_001972.4 , XM_011527775.1 , XM_011546888.1 , XM_011527776.1
Accession Version	XM_011527775.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 804bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	neutrophil elastase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCCTCG GCCGCCGACT CGCGTGTCTT TTCCTCGCCT GTGTCCTGCC GGCCTTGCTG 
CTGGGGGGCA CCGCGCTGGC CTCGGAGATT GTGGGGGGCC GGCGAGCGCG GCCCCACGCG 
TGGCCCTTCA TGGTGTCCCT GCAGCTGCGC GGAGGCCACT TCTGCGGCGC CACCCTGATT 
GCGCCCAACT TCGTCATGTC GGCCGCGCAC TGCGTGGCGA ATGTAAACGT CCGCGCGGTG 
CGGGTGGTCC TGGGAGCCCA TAACCTCTCG CGGCGGGAGC CCACCCGGCA GGTGTTCGCC 
GTGCAGCGCA TCTTCGAAAA CGGCTACGAC CCCGTAAACT TGCTCAACGA CATCGTGATT 
CTCCAGCTCA ACGGGTCGGC CACCATCAAC GCCAACGTGC AGGTGGCCCA GCTGCCGGCT 
CAGGGACGCC GCCTGGGCAA CGGGGTGCAG TGCCTGGCCA TGGGCTGGGG CCTTCTGGGC 
AGGAACCGTG GGATCGCCAG CGTCCTGCAG GAGCTCAACG TGACGGTGGT GACGTCCCTC 
TGCCGTCGCA GCAACGTCTG CACTCTCGTG AGGGGCCGGC AGGCCGGCGT CTGTTTCGGG 
GACTCCGGCA GCCCCTTGGT CTGCAACGGG CTAATCCACG GAATTGCCTC CTTCGTCCGG 
GGAGGCTGCG CCTCAGGGCT CTACCCCGAT GCCTTTGCCC CGGTGGCACA GTTTGTAAAC 
TGGATCGACT CTATCATCCA ACGCTCCGAG GACAACCCCT GTCCCCACCC CCGGGACCCG 
GACCCGGCCA GCAGGACCCA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011526077.1
CDS	798..1601
Translation	MTLGRRLACLFLACVLPALLLGGTALASEIVGGRRARPHAWPFMVSLQLRGGHFCGATLIAPNFVMSAAHCVANVNVRAVRVVLGAHNLSRREPTRQVFAVQRIFENGYDPVNLLNDIVILQLNGSATINANVQVAQLPAQGRRLGNGVQCLAMGWGLLGRNRGIASVLQELNVTVVTSLCRRSNVCTLVRGRQAGVCFGDSGSPLVCNGLIHGIASFVRGGCASGLYPDAFAPVAQFVNWIDSIIQRSEDNPCPHPRDPDPASRTH

Target ORF information:

RefSeq Version	XM_011527775.1
Organism	Homo sapiens(human)
Definition	Homo sapiens elastase, neutrophil expressed (ELANE), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011527775.1

ORF Insert Sequence:

ATGACCCTCG GCCGCCGACT CGCGTGTCTT TTCCTCGCCT GTGTCCTGCC GGCCTTGCTG 
CTGGGGGGCA CCGCGCTGGC CTCGGAGATT GTGGGGGGCC GGCGAGCGCG GCCCCACGCG 
TGGCCCTTCA TGGTGTCCCT GCAGCTGCGC GGAGGCCACT TCTGCGGCGC CACCCTGATT 
GCGCCCAACT TCGTCATGTC GGCCGCGCAC TGCGTGGCGA ATGTAAACGT CCGCGCGGTG 
CGGGTGGTCC TGGGAGCCCA TAACCTCTCG CGGCGGGAGC CCACCCGGCA GGTGTTCGCC 
GTGCAGCGCA TCTTCGAAAA CGGCTACGAC CCCGTAAACT TGCTCAACGA CATCGTGATT 
CTCCAGCTCA ACGGGTCGGC CACCATCAAC GCCAACGTGC AGGTGGCCCA GCTGCCGGCT 
CAGGGACGCC GCCTGGGCAA CGGGGTGCAG TGCCTGGCCA TGGGCTGGGG CCTTCTGGGC 
AGGAACCGTG GGATCGCCAG CGTCCTGCAG GAGCTCAACG TGACGGTGGT GACGTCCCTC 
TGCCGTCGCA GCAACGTCTG CACTCTCGTG AGGGGCCGGC AGGCCGGCGT CTGTTTCGGG 
GACTCCGGCA GCCCCTTGGT CTGCAACGGG CTAATCCACG GAATTGCCTC CTTCGTCCGG 
GGAGGCTGCG CCTCAGGGCT CTACCCCGAT GCCTTTGCCC CGGTGGCACA GTTTGTAAAC 
TGGATCGACT CTATCATCCA ACGCTCCGAG GACAACCCCT GTCCCCACCC CCGGGACCCG 
GACCCGGCCA GCAGGACCCA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ELANE ( XM_011546888.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011545190.1 Homo sapiens elastase, neutrophil expressed (ELANE), transcript variant X2, mRNA.

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CloneID	OHu19461
Clone ID Related Accession (Same CDS sequence)	NM_001972.2 , NM_001972.3 , NM_001972.4 , XM_011527775.1 , XM_011546888.1 , XM_011527776.1
Accession Version	XM_011546888.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 804bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-11
Organism	Homo sapiens(Human)
Product	neutrophil elastase isoform X1
Comment

ATGACCCTCG GCCGCCGACT CGCGTGTCTT TTCCTCGCCT GTGTCCTGCC GGCCTTGCTG 
CTGGGGGGCA CCGCGCTGGC CTCGGAGATT GTGGGGGGCC GGCGAGCGCG GCCCCACGCG 
TGGCCCTTCA TGGTGTCCCT GCAGCTGCGC GGAGGCCACT TCTGCGGCGC CACCCTGATT 
GCGCCCAACT TCGTCATGTC GGCCGCGCAC TGCGTGGCGA ATGTAAACGT CCGCGCGGTG 
CGGGTGGTCC TGGGAGCCCA TAACCTCTCG CGGCGGGAGC CCACCCGGCA GGTGTTCGCC 
GTGCAGCGCA TCTTCGAAAA CGGCTACGAC CCCGTAAACT TGCTCAACGA CATCGTGATT 
CTCCAGCTCA ACGGGTCGGC CACCATCAAC GCCAACGTGC AGGTGGCCCA GCTGCCGGCT 
CAGGGACGCC GCCTGGGCAA CGGGGTGCAG TGCCTGGCCA TGGGCTGGGG CCTTCTGGGC 
AGGAACCGTG GGATCGCCAG CGTCCTGCAG GAGCTCAACG TGACGGTGGT GACGTCCCTC 
TGCCGTCGCA GCAACGTCTG CACTCTCGTG AGGGGCCGGC AGGCCGGCGT CTGTTTCGGG 
GACTCCGGCA GCCCCTTGGT CTGCAACGGG CTAATCCACG GAATTGCCTC CTTCGTCCGG 
GGAGGCTGCG CCTCAGGGCT CTACCCCGAT GCCTTTGCCC CGGTGGCACA GTTTGTAAAC 
TGGATCGACT CTATCATCCA ACGCTCCGAG GACAACCCCT GTCCCCACCC CCGGGACCCG 
GACCCGGCCA GCAGGACCCA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011545190.1
Translation	MTLGRRLACLFLACVLPALLLGGTALASEIVGGRRARPHAWPFMVSLQLRGGHFCGATLIAPNFVMSAAHCVANVNVRAVRVVLGAHNLSRREPTRQVFAVQRIFENGYDPVNLLNDIVILQLNGSATINANVQVAQLPAQGRRLGNGVQCLAMGWGLLGRNRGIASVLQELNVTVVTSLCRRSNVCTLVRGRQAGVCFGDSGSPLVCNGLIHGIASFVRGGCASGLYPDAFAPVAQFVNWIDSIIQRSEDNPCPHPRDPDPASRTH

Target ORF information:

RefSeq Version	XM_011546888.1
Organism	Homo sapiens(Human)
Definition	Homo sapiens elastase, neutrophil expressed (ELANE), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011546888.1

ORF Insert Sequence:

ATGACCCTCG GCCGCCGACT CGCGTGTCTT TTCCTCGCCT GTGTCCTGCC GGCCTTGCTG 
CTGGGGGGCA CCGCGCTGGC CTCGGAGATT GTGGGGGGCC GGCGAGCGCG GCCCCACGCG 
TGGCCCTTCA TGGTGTCCCT GCAGCTGCGC GGAGGCCACT TCTGCGGCGC CACCCTGATT 
GCGCCCAACT TCGTCATGTC GGCCGCGCAC TGCGTGGCGA ATGTAAACGT CCGCGCGGTG 
CGGGTGGTCC TGGGAGCCCA TAACCTCTCG CGGCGGGAGC CCACCCGGCA GGTGTTCGCC 
GTGCAGCGCA TCTTCGAAAA CGGCTACGAC CCCGTAAACT TGCTCAACGA CATCGTGATT 
CTCCAGCTCA ACGGGTCGGC CACCATCAAC GCCAACGTGC AGGTGGCCCA GCTGCCGGCT 
CAGGGACGCC GCCTGGGCAA CGGGGTGCAG TGCCTGGCCA TGGGCTGGGG CCTTCTGGGC 
AGGAACCGTG GGATCGCCAG CGTCCTGCAG GAGCTCAACG TGACGGTGGT GACGTCCCTC 
TGCCGTCGCA GCAACGTCTG CACTCTCGTG AGGGGCCGGC AGGCCGGCGT CTGTTTCGGG 
GACTCCGGCA GCCCCTTGGT CTGCAACGGG CTAATCCACG GAATTGCCTC CTTCGTCCGG 
GGAGGCTGCG CCTCAGGGCT CTACCCCGAT GCCTTTGCCC CGGTGGCACA GTTTGTAAAC 
TGGATCGACT CTATCATCCA ACGCTCCGAG GACAACCCCT GTCCCCACCC CCGGGACCCG 
GACCCGGCCA GCAGGACCCA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ELANE ( XM_011527776.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011526078.1 Homo sapiens elastase, neutrophil expressed (ELANE), transcript variant X2, mRNA.

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CloneID	OHu19461
Clone ID Related Accession (Same CDS sequence)	NM_001972.2 , NM_001972.3 , NM_001972.4 , XM_011527775.1 , XM_011546888.1 , XM_011527776.1
Accession Version	XM_011527776.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 804bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	neutrophil elastase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCCTCG GCCGCCGACT CGCGTGTCTT TTCCTCGCCT GTGTCCTGCC GGCCTTGCTG 
CTGGGGGGCA CCGCGCTGGC CTCGGAGATT GTGGGGGGCC GGCGAGCGCG GCCCCACGCG 
TGGCCCTTCA TGGTGTCCCT GCAGCTGCGC GGAGGCCACT TCTGCGGCGC CACCCTGATT 
GCGCCCAACT TCGTCATGTC GGCCGCGCAC TGCGTGGCGA ATGTAAACGT CCGCGCGGTG 
CGGGTGGTCC TGGGAGCCCA TAACCTCTCG CGGCGGGAGC CCACCCGGCA GGTGTTCGCC 
GTGCAGCGCA TCTTCGAAAA CGGCTACGAC CCCGTAAACT TGCTCAACGA CATCGTGATT 
CTCCAGCTCA ACGGGTCGGC CACCATCAAC GCCAACGTGC AGGTGGCCCA GCTGCCGGCT 
CAGGGACGCC GCCTGGGCAA CGGGGTGCAG TGCCTGGCCA TGGGCTGGGG CCTTCTGGGC 
AGGAACCGTG GGATCGCCAG CGTCCTGCAG GAGCTCAACG TGACGGTGGT GACGTCCCTC 
TGCCGTCGCA GCAACGTCTG CACTCTCGTG AGGGGCCGGC AGGCCGGCGT CTGTTTCGGG 
GACTCCGGCA GCCCCTTGGT CTGCAACGGG CTAATCCACG GAATTGCCTC CTTCGTCCGG 
GGAGGCTGCG CCTCAGGGCT CTACCCCGAT GCCTTTGCCC CGGTGGCACA GTTTGTAAAC 
TGGATCGACT CTATCATCCA ACGCTCCGAG GACAACCCCT GTCCCCACCC CCGGGACCCG 
GACCCGGCCA GCAGGACCCA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011526078.1
CDS	137..940
Translation	MTLGRRLACLFLACVLPALLLGGTALASEIVGGRRARPHAWPFMVSLQLRGGHFCGATLIAPNFVMSAAHCVANVNVRAVRVVLGAHNLSRREPTRQVFAVQRIFENGYDPVNLLNDIVILQLNGSATINANVQVAQLPAQGRRLGNGVQCLAMGWGLLGRNRGIASVLQELNVTVVTSLCRRSNVCTLVRGRQAGVCFGDSGSPLVCNGLIHGIASFVRGGCASGLYPDAFAPVAQFVNWIDSIIQRSEDNPCPHPRDPDPASRTH

Target ORF information:

RefSeq Version	XM_011527776.1
Organism	Homo sapiens(human)
Definition	Homo sapiens elastase, neutrophil expressed (ELANE), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011527776.1

ORF Insert Sequence:

ATGACCCTCG GCCGCCGACT CGCGTGTCTT TTCCTCGCCT GTGTCCTGCC GGCCTTGCTG 
CTGGGGGGCA CCGCGCTGGC CTCGGAGATT GTGGGGGGCC GGCGAGCGCG GCCCCACGCG 
TGGCCCTTCA TGGTGTCCCT GCAGCTGCGC GGAGGCCACT TCTGCGGCGC CACCCTGATT 
GCGCCCAACT TCGTCATGTC GGCCGCGCAC TGCGTGGCGA ATGTAAACGT CCGCGCGGTG 
CGGGTGGTCC TGGGAGCCCA TAACCTCTCG CGGCGGGAGC CCACCCGGCA GGTGTTCGCC 
GTGCAGCGCA TCTTCGAAAA CGGCTACGAC CCCGTAAACT TGCTCAACGA CATCGTGATT 
CTCCAGCTCA ACGGGTCGGC CACCATCAAC GCCAACGTGC AGGTGGCCCA GCTGCCGGCT 
CAGGGACGCC GCCTGGGCAA CGGGGTGCAG TGCCTGGCCA TGGGCTGGGG CCTTCTGGGC 
AGGAACCGTG GGATCGCCAG CGTCCTGCAG GAGCTCAACG TGACGGTGGT GACGTCCCTC 
TGCCGTCGCA GCAACGTCTG CACTCTCGTG AGGGGCCGGC AGGCCGGCGT CTGTTTCGGG 
GACTCCGGCA GCCCCTTGGT CTGCAACGGG CTAATCCACG GAATTGCCTC CTTCGTCCGG 
GGAGGCTGCG CCTCAGGGCT CTACCCCGAT GCCTTTGCCC CGGTGGCACA GTTTGTAAAC 
TGGATCGACT CTATCATCCA ACGCTCCGAG GACAACCCCT GTCCCCACCC CCGGGACCCG 
GACCCGGCCA GCAGGACCCA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Elastase inhibitors as potential therapies for
Journal of leukocyte biology102(4)1143-1151(2017 Oct)
Makaryan V,Kelley ML,Fletcher B,Bolyard AA,Aprikyan AA,Dale DC

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ELANE cDNA ORF clone, Homo sapiens(human)

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Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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