EIF5 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	EIF5
Entrez Gene ID	1983
Full Name	eukaryotic translation initiation factor 5
Synonyms	EIF-5,EIF-5A
General protein information	Preferred Names eukaryotic translation initiation factor 5 Names eukaryotic translation initiation factor 5
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 14 Map Location: 14q32.32
Summary	Eukaryotic translation initiation factor-5 (EIF5) interacts with the 40S initiation complex to promote hydrolysis of bound GTP with concomitant joining of the 60S ribosomal subunit to the 40S initiation complex. The resulting functional 80S ribosomal initiation complex is then active in peptidyl transfer and chain elongations (summary by Si et al., 1996 [PubMed 8663286]).[supplied by OMIM, May 2010].
Disorder MIM:	601710

mRNA and Protein(s)

mRNA	Protein	Name
NM_001969.4	NP_001960.2	eukaryotic translation initiation factor 5
NM_183004.5	NP_892116.2	eukaryotic translation initiation factor 5
NM_183004.4	NP_892116.2	eukaryotic translation initiation factor 5
NM_001969.5	NP_001960.2	eukaryotic translation initiation factor 5

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	EIF5	XP_868610.1
Caenorhabditis elegans (roundworm)	C37C3.2	NP_505024.2
Rattus norvegicus (Norway rat)	Eif5	NP_064460.1
Eremothecium gossypii	AGOS_AER400C	NP_985255.2
Homo sapiens (human)	EIF5	NP_892116.2
Pan troglodytes (chimpanzee)	EIF5	XP_001136120.1
Mus musculus (house mouse)	Eif5	NP_829887.1
Danio rerio (zebrafish)	eif5	NP_955885.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011203	XP_309438.2
Kluyveromyces lactis	KLLA0E06095g	XP_454221.1
Schizosaccharomyces pombe (fission yeast)	SPAC2F7.05c	NP_592976.1
Neurospora crassa	NCU00366	XP_957252.2
Xenopus tropicalis (tropical clawed frog)	eif5	NP_001008209.1
	LOC697538	XP_001086107.2
Gallus gallus (chicken)	EIF5	NP_001006488.1
Drosophila melanogaster (fruit fly)	eIF5	NP_996480.1
Saccharomyces cerevisiae (baker's yeast)	TIF5	NP_015366.1
Magnaporthe oryzae (rice blast fungus)	MGG_08866	XP_003713892.1
Bos taurus (cattle)	EIF5	NP_001180152.1

Gene Ontology
Bibliography

Related articles in PubMed

Competition between translation initiation factor eIF5 and its mimic protein 5MP determines non-AUG initiation rate genome-wide.
Tang L, Morris J, Wan J, Moore C, Fujita Y, Gillaspie S, Aube E, Nanda J, Marques M, Jangal M, Anderson A, Cox C, Hiraishi H, Dong L, Saito H, Singh CR, Witcher M, Topisirovic I, Qian SB, Asano K
Nucleic acids research45(20)11941-11953(2017 Nov)

A Case-Matched Gender Comparison Transcriptomic Screen Identifies eIF4E and eIF5 as Potential Prognostic Markers in Male Breast Cancer.
Humphries MP, Sundara Rajan S, Droop A, Suleman CAB, Carbone C, Nilsson C, Honarpisheh H, Cserni G, Dent J, Fulford L, Jordan LB, Jones JL, Kanthan R, Litwiniuk M, Di Benedetto A, Mottolese M, Provenzano E, Shousha S, Stephens M, Walker RA, Kulka J, Ellis IO, Jeffery M, Thygesen HH, Cappelletti V, Daidone MG, Hedenfalk IA, Fj?llskog ML, Melisi D, Stead LF, Shaaban AM, Speirs V
Clinical cancer research : an official journal of the American Association for Cancer Research23(10)2575-2583(2017 May)

Defect in the GTPase activating protein (GAP) function of eIF5 causes repression of GCN4 translation.
Antony A C, Alone PV
Biochemical and biophysical research communications486(4)1110-1115(2017 05)

Overexpression of eIF5 or its protein mimic 5MP perturbs eIF2 function and induces ATF4 translation through delayed re-initiation.
Kozel C, Thompson B, Hustak S, Moore C, Nakashima A, Singh CR, Reid M, Cox C, Papadopoulos E, Luna RE, Anderson A, Tagami H, Hiraishi H, Slone EA, Yoshino KI, Asano M, Gillaspie S, Nietfeld J, Perchellet JP, Rothenburg S, Masai H, Wagner G, Beeser A, Kikkawa U, Fleming SD, Asano K
Nucleic acids research44(18)8704-8713(2016 Oct)

Sliding of a 43S ribosomal complex from the recognized AUG codon triggered by a delay in eIF2-bound GTP hydrolysis.
Terenin IM, Akulich KA, Andreev DE, Polyanskaya SA, Shatsky IN, Dmitriev SE
Nucleic acids research44(4)1882-93(2016 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data show that eIF5-mimic protein (5MP) represses non-AUG translation by competing with translation initiation factor 5 (eIF5) for the Met-tRNAi-binding factor eIF2.
Title: Competition between translation initiation factor eIF5 and its mimic protein 5MP determines non-AUG initiation rate genome-wide.

The down-regulation of the GCN4 expression (Gcn(-) phenotype) in the eIF5(G31R) mutant was not because of leaky scanning defects; rather was due to the utilization of upUUG initiation codons at the 5' regulatory region present between uORF1 and the main GCN4 ORF.
Title: Defect in the GTPase activating protein (GAP) function of eIF5 causes repression of GCN4 translation.

overexpression of eIF5 and 5MP induces translation of ATF4
Title: Overexpression of eIF5 or its protein mimic 5MP perturbs eIF2 function and induces ATF4 translation through delayed re-initiation.

it is eIF5-induced GTP hydrolysis and Pi release that irreversibly trap the 48S complex, and this complex is further stabilized by eIF5B and 60S joining.
Title: Sliding of a 43S ribosomal complex from the recognized AUG codon triggered by a delay in eIF2-bound GTP hydrolysis.

The N-terminal tail of eIF1A mediates the interaction with eIF5 and eIF1.
Title: The interaction between eukaryotic initiation factor 1A and eIF5 retains eIF1 within scanning preinitiation complexes.

The following EIF5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EIF5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu12534	NM_183004.5 Latest version!	Homo sapiens eukaryotic translation initiation factor 5 (EIF5), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
OHu12534	NM_001969.5 Latest version!	Homo sapiens eukaryotic translation initiation factor 5 (EIF5), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
View Old Accession Versions >>
OHu12534	NM_183004.4	Homo sapiens eukaryotic translation initiation factor 5 (EIF5), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
OHu12534	NM_001969.4	Homo sapiens eukaryotic translation initiation factor 5 (EIF5), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

EIF5 ( NM_001969.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_001960.2 Homo sapiens eukaryotic translation initiation factor 5 (EIF5), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu12534
Clone ID Related Accession (Same CDS sequence)	NM_001969.4 , NM_183004.5 , NM_183004.4 , NM_001969.5
Accession Version	NM_001969.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1296bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-17
Organism	Homo sapiens(human)
Product	eukaryotic translation initiation factor 5
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DB458085.1, AL080102.1, AK026933.1, AL138976.5 and AK027240.1. On May 15, 2012 this sequence version replaced NM_001969.3. Transcript Variant: This variant (1) represents the longer transcript. Variants 1 and 2 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.29329.1, SRR1660809.63643.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCTGTCA ATGTCAACCG CAGCGTGTCA GACCAGTTCT ATCGCTACAA GATGCCCCGT 
CTGATTGCCA AGGTTGAGGG CAAAGGCAAT GGAATCAAGA CAGTTATAGT CAACATGGTT 
GACGTTGCAA AGGCGCTTAA TCGGCCTCCA ACGTATCCCA CCAAATATTT TGGTTGTGAG 
CTGGGAGCAC AGACCCAGTT TGATGTTAAG AATGACCGTT ACATTGTCAA TGGATCTCAT 
GAGGCGAATA AGCTGCAAGA CATGTTGGAT GGATTCATTA AAAAATTTGT TCTCTGTCCT 
GAATGTGAGA ATCCTGAAAC AGATTTGCAT GTCAATCCAA AGAAGCAAAC AATAGGTAAT 
TCTTGTAAAG CCTGTGGCTA TCGAGGCATG CTTGACACAC ATCATAAACT CTGCACATTC 
ATTCTCAAAA ACCCACCTGA GAATAGTGAC AGTGGTACAG GAAAGAAAGA AAAAGAAAAG 
AAAAACAGAA AGGGCAAAGA CAAGGAAAAT GGCTCCGTAT CCAGCAGTGA GACACCACCA 
CCACCACCAC CACCAAATGA AATTAATCCT CCTCCACATA CAATGGAAGA AGAGGAGGAT 
GATGACTGGG GAGAAGATAC AACTGAGGAA GCTCAAAGGC GTCGAATGGA TGAAATCAGT 
GACCATGCAA AAGTTCTGAC ACTCAGTGAT GATTTGGAAA GAACAATTGA GGAGAGGGTC 
AATATCCTCT TTGATTTTGT TAAGAAAAAG AAAGAAGAGG GTGTTATTGA TTCATCTGAC 
AAAGAAATCG TTGCTGAAGC AGAAAGACTG GATGTAAAAG CCATGGGCCC TCTTGTTCTA 
ACTGAAGTTC TTTTTAATGA GAAGATTAGA GAACAGATTA AGAAATACAG GCGCCATTTC 
CTACGATTTT GTCACAACAA CAAAAAAGCC CAACGGTACC TTCTTCATGG TTTGGAGTGT 
GTGGTAGCAA TGCATCAAGC TCAGCTTATC TCCAAGATTC CACATATCTT GAAGGAGATG 
TACGATGCAG ACCTTTTAGA AGAAGAGGTC ATCATCAGCT GGTCGGAAAA GGCCTCTAAG 
AAATATGTCT CCAAAGAACT TGCCAAAGAG ATTCGTGTCA AAGCAGAACC ATTTATAAAA 
TGGTTGAAGG AGGCAGAGGA AGAATCTTCT GGTGGCGAAG AAGAAGATGA AGATGAGAAC 
ATTGAGGTGG TGTATTCGAA GGCTGCCAGT GTACCGAAAG TTGAGACTGT AAAGTCAGAC 
AACAAGGATG ACGACATCGA TATTGATGCC ATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001960.2
CDS	677..1972
Translation	MSVNVNRSVSDQFYRYKMPRLIAKVEGKGNGIKTVIVNMVDVAKALNRPPTYPTKYFGCELGAQTQFDVKNDRYIVNGSHEANKLQDMLDGFIKKFVLCPECENPETDLHVNPKKQTIGNSCKACGYRGMLDTHHKLCTFILKNPPENSDSGTGKKEKEKKNRKGKDKENGSVSSSETPPPPPPPNEINPPPHTMEEEEDDDWGEDTTEEAQRRRMDEISDHAKVLTLSDDLERTIEERVNILFDFVKKKKEEGVIDSSDKEIVAEAERLDVKAMGPLVLTEVLFNEKIREQIKKYRRHFLRFCHNNKKAQRYLLHGLECVVAMHQAQLISKIPHILKEMYDADLLEEEVIISWSEKASKKYVSKELAKEIRVKAEPFIKWLKEAEEESSGGEEEDEDENIEVVYSKAASVPKVETVKSDNKDDDIDIDAI

Target ORF information:

RefSeq Version	NM_001969.4
Organism	Homo sapiens(human)
Definition	Homo sapiens eukaryotic translation initiation factor 5 (EIF5), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001969.4

ORF Insert Sequence:

ATGTCTGTCA ATGTCAACCG CAGCGTGTCA GACCAGTTCT ATCGCTACAA GATGCCCCGT 
CTGATTGCCA AGGTTGAGGG CAAAGGCAAT GGAATCAAGA CAGTTATAGT CAACATGGTT 
GACGTTGCAA AGGCGCTTAA TCGGCCTCCA ACGTATCCCA CCAAATATTT TGGTTGTGAG 
CTGGGAGCAC AGACCCAGTT TGATGTTAAG AATGACCGTT ACATTGTCAA TGGATCTCAT 
GAGGCGAATA AGCTGCAAGA CATGTTGGAT GGATTCATTA AAAAATTTGT TCTCTGTCCT 
GAATGTGAGA ATCCTGAAAC AGATTTGCAT GTCAATCCAA AGAAGCAAAC AATAGGTAAT 
TCTTGTAAAG CCTGTGGCTA TCGAGGCATG CTTGACACAC ATCATAAACT CTGCACATTC 
ATTCTCAAAA ACCCACCTGA GAATAGTGAC AGTGGTACAG GAAAGAAAGA AAAAGAAAAG 
AAAAACAGAA AGGGCAAAGA CAAGGAAAAT GGCTCCGTAT CCAGCAGTGA GACACCACCA 
CCACCACCAC CACCAAATGA AATTAATCCT CCTCCACATA CAATGGAAGA AGAGGAGGAT 
GATGACTGGG GAGAAGATAC AACTGAGGAA GCTCAAAGGC GTCGAATGGA TGAAATCAGT 
GACCATGCAA AAGTTCTGAC ACTCAGTGAT GATTTGGAAA GAACAATTGA GGAGAGGGTC 
AATATCCTCT TTGATTTTGT TAAGAAAAAG AAAGAAGAGG GTGTTATTGA TTCATCTGAC 
AAAGAAATCG TTGCTGAAGC AGAAAGACTG GATGTAAAAG CCATGGGCCC TCTTGTTCTA 
ACTGAAGTTC TTTTTAATGA GAAGATTAGA GAACAGATTA AGAAATACAG GCGCCATTTC 
CTACGATTTT GTCACAACAA CAAAAAAGCC CAACGGTACC TTCTTCATGG TTTGGAGTGT 
GTGGTAGCAA TGCATCAAGC TCAGCTTATC TCCAAGATTC CACATATCTT GAAGGAGATG 
TACGATGCAG ACCTTTTAGA AGAAGAGGTC ATCATCAGCT GGTCGGAAAA GGCCTCTAAG 
AAATATGTCT CCAAAGAACT TGCCAAAGAG ATTCGTGTCA AAGCAGAACC ATTTATAAAA 
TGGTTGAAGG AGGCAGAGGA AGAATCTTCT GGTGGCGAAG AAGAAGATGA AGATGAGAAC 
ATTGAGGTGG TGTATTCGAA GGCTGCCAGT GTACCGAAAG TTGAGACTGT AAAGTCAGAC 
AACAAGGATG ACGACATCGA TATTGATGCC ATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EIF5 ( NM_183004.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_892116.2 Homo sapiens eukaryotic translation initiation factor 5 (EIF5), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu12534
Clone ID Related Accession (Same CDS sequence)	NM_001969.4 , NM_183004.5 , NM_183004.4 , NM_001969.5
Accession Version	NM_183004.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1296bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-30
Organism	Homo sapiens(human)
Product	eukaryotic translation initiation factor 5
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL138976.5. On May 31, 2019 this sequence version replaced NM_183004.4. Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 and 2 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.163340.1, SRR1803617.260292.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2144120 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCTGTCA ATGTCAACCG CAGCGTGTCA GACCAGTTCT ATCGCTACAA GATGCCCCGT 
CTGATTGCCA AGGTTGAGGG CAAAGGCAAT GGAATCAAGA CAGTTATAGT CAACATGGTT 
GACGTTGCAA AGGCGCTTAA TCGGCCTCCA ACGTATCCCA CCAAATATTT TGGTTGTGAG 
CTGGGAGCAC AGACCCAGTT TGATGTTAAG AATGACCGTT ACATTGTCAA TGGATCTCAT 
GAGGCGAATA AGCTGCAAGA CATGTTGGAT GGATTCATTA AAAAATTTGT TCTCTGTCCT 
GAATGTGAGA ATCCTGAAAC AGATTTGCAT GTCAATCCAA AGAAGCAAAC AATAGGTAAT 
TCTTGTAAAG CCTGTGGCTA TCGAGGCATG CTTGACACAC ATCATAAACT CTGCACATTC 
ATTCTCAAAA ACCCACCTGA GAATAGTGAC AGTGGTACAG GAAAGAAAGA AAAAGAAAAG 
AAAAACAGAA AGGGCAAAGA CAAGGAAAAT GGCTCCGTAT CCAGCAGTGA GACACCACCA 
CCACCACCAC CACCAAATGA AATTAATCCT CCTCCACATA CAATGGAAGA AGAGGAGGAT 
GATGACTGGG GAGAAGATAC AACTGAGGAA GCTCAAAGGC GTCGAATGGA TGAAATCAGT 
GACCATGCAA AAGTTCTGAC ACTCAGTGAT GATTTGGAAA GAACAATTGA GGAGAGGGTC 
AATATCCTCT TTGATTTTGT TAAGAAAAAG AAAGAAGAGG GTGTTATTGA TTCATCTGAC 
AAAGAAATCG TTGCTGAAGC AGAAAGACTG GATGTAAAAG CCATGGGCCC TCTTGTTCTA 
ACTGAAGTTC TTTTTAATGA GAAGATTAGA GAACAGATTA AGAAATACAG GCGCCATTTC 
CTACGATTTT GTCACAACAA CAAAAAAGCC CAACGGTACC TTCTTCATGG TTTGGAGTGT 
GTGGTAGCAA TGCATCAAGC TCAGCTTATC TCCAAGATTC CACATATCTT GAAGGAGATG 
TACGATGCAG ACCTTTTAGA AGAAGAGGTC ATCATCAGCT GGTCGGAAAA GGCCTCTAAG 
AAATATGTCT CCAAAGAACT TGCCAAAGAG ATTCGTGTCA AAGCAGAACC ATTTATAAAA 
TGGTTGAAGG AGGCAGAGGA AGAATCTTCT GGTGGCGAAG AAGAAGATGA AGATGAGAAC 
ATTGAGGTGG TGTATTCGAA GGCTGCCAGT GTACCGAAAG TTGAGACTGT AAAGTCAGAC 
AACAAGGATG ACGACATCGA TATTGATGCC ATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_892116.2
CDS	507..1802
Translation	MSVNVNRSVSDQFYRYKMPRLIAKVEGKGNGIKTVIVNMVDVAKALNRPPTYPTKYFGCELGAQTQFDVKNDRYIVNGSHEANKLQDMLDGFIKKFVLCPECENPETDLHVNPKKQTIGNSCKACGYRGMLDTHHKLCTFILKNPPENSDSGTGKKEKEKKNRKGKDKENGSVSSSETPPPPPPPNEINPPPHTMEEEEDDDWGEDTTEEAQRRRMDEISDHAKVLTLSDDLERTIEERVNILFDFVKKKKEEGVIDSSDKEIVAEAERLDVKAMGPLVLTEVLFNEKIREQIKKYRRHFLRFCHNNKKAQRYLLHGLECVVAMHQAQLISKIPHILKEMYDADLLEEEVIISWSEKASKKYVSKELAKEIRVKAEPFIKWLKEAEEESSGGEEEDEDENIEVVYSKAASVPKVETVKSDNKDDDIDIDAI

Target ORF information:

RefSeq Version	NM_183004.5
Organism	Homo sapiens(human)
Definition	Homo sapiens eukaryotic translation initiation factor 5 (EIF5), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_183004.5

ORF Insert Sequence:

ATGTCTGTCA ATGTCAACCG CAGCGTGTCA GACCAGTTCT ATCGCTACAA GATGCCCCGT 
CTGATTGCCA AGGTTGAGGG CAAAGGCAAT GGAATCAAGA CAGTTATAGT CAACATGGTT 
GACGTTGCAA AGGCGCTTAA TCGGCCTCCA ACGTATCCCA CCAAATATTT TGGTTGTGAG 
CTGGGAGCAC AGACCCAGTT TGATGTTAAG AATGACCGTT ACATTGTCAA TGGATCTCAT 
GAGGCGAATA AGCTGCAAGA CATGTTGGAT GGATTCATTA AAAAATTTGT TCTCTGTCCT 
GAATGTGAGA ATCCTGAAAC AGATTTGCAT GTCAATCCAA AGAAGCAAAC AATAGGTAAT 
TCTTGTAAAG CCTGTGGCTA TCGAGGCATG CTTGACACAC ATCATAAACT CTGCACATTC 
ATTCTCAAAA ACCCACCTGA GAATAGTGAC AGTGGTACAG GAAAGAAAGA AAAAGAAAAG 
AAAAACAGAA AGGGCAAAGA CAAGGAAAAT GGCTCCGTAT CCAGCAGTGA GACACCACCA 
CCACCACCAC CACCAAATGA AATTAATCCT CCTCCACATA CAATGGAAGA AGAGGAGGAT 
GATGACTGGG GAGAAGATAC AACTGAGGAA GCTCAAAGGC GTCGAATGGA TGAAATCAGT 
GACCATGCAA AAGTTCTGAC ACTCAGTGAT GATTTGGAAA GAACAATTGA GGAGAGGGTC 
AATATCCTCT TTGATTTTGT TAAGAAAAAG AAAGAAGAGG GTGTTATTGA TTCATCTGAC 
AAAGAAATCG TTGCTGAAGC AGAAAGACTG GATGTAAAAG CCATGGGCCC TCTTGTTCTA 
ACTGAAGTTC TTTTTAATGA GAAGATTAGA GAACAGATTA AGAAATACAG GCGCCATTTC 
CTACGATTTT GTCACAACAA CAAAAAAGCC CAACGGTACC TTCTTCATGG TTTGGAGTGT 
GTGGTAGCAA TGCATCAAGC TCAGCTTATC TCCAAGATTC CACATATCTT GAAGGAGATG 
TACGATGCAG ACCTTTTAGA AGAAGAGGTC ATCATCAGCT GGTCGGAAAA GGCCTCTAAG 
AAATATGTCT CCAAAGAACT TGCCAAAGAG ATTCGTGTCA AAGCAGAACC ATTTATAAAA 
TGGTTGAAGG AGGCAGAGGA AGAATCTTCT GGTGGCGAAG AAGAAGATGA AGATGAGAAC 
ATTGAGGTGG TGTATTCGAA GGCTGCCAGT GTACCGAAAG TTGAGACTGT AAAGTCAGAC 
AACAAGGATG ACGACATCGA TATTGATGCC ATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EIF5 ( NM_183004.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_892116.2 Homo sapiens eukaryotic translation initiation factor 5 (EIF5), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu12534
Clone ID Related Accession (Same CDS sequence)	NM_001969.4 , NM_183004.5 , NM_183004.4 , NM_001969.5
Accession Version	NM_183004.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1296bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-24
Organism	Homo sapiens(human)
Product	eukaryotic translation initiation factor 5
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL138976.5, BC032866.2 and AK027240.1. On May 15, 2012 this sequence version replaced NM_183004.3. Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 and 2 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.10641.1, SRR1803611.163340.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2144120 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCTGTCA ATGTCAACCG CAGCGTGTCA GACCAGTTCT ATCGCTACAA GATGCCCCGT 
CTGATTGCCA AGGTTGAGGG CAAAGGCAAT GGAATCAAGA CAGTTATAGT CAACATGGTT 
GACGTTGCAA AGGCGCTTAA TCGGCCTCCA ACGTATCCCA CCAAATATTT TGGTTGTGAG 
CTGGGAGCAC AGACCCAGTT TGATGTTAAG AATGACCGTT ACATTGTCAA TGGATCTCAT 
GAGGCGAATA AGCTGCAAGA CATGTTGGAT GGATTCATTA AAAAATTTGT TCTCTGTCCT 
GAATGTGAGA ATCCTGAAAC AGATTTGCAT GTCAATCCAA AGAAGCAAAC AATAGGTAAT 
TCTTGTAAAG CCTGTGGCTA TCGAGGCATG CTTGACACAC ATCATAAACT CTGCACATTC 
ATTCTCAAAA ACCCACCTGA GAATAGTGAC AGTGGTACAG GAAAGAAAGA AAAAGAAAAG 
AAAAACAGAA AGGGCAAAGA CAAGGAAAAT GGCTCCGTAT CCAGCAGTGA GACACCACCA 
CCACCACCAC CACCAAATGA AATTAATCCT CCTCCACATA CAATGGAAGA AGAGGAGGAT 
GATGACTGGG GAGAAGATAC AACTGAGGAA GCTCAAAGGC GTCGAATGGA TGAAATCAGT 
GACCATGCAA AAGTTCTGAC ACTCAGTGAT GATTTGGAAA GAACAATTGA GGAGAGGGTC 
AATATCCTCT TTGATTTTGT TAAGAAAAAG AAAGAAGAGG GTGTTATTGA TTCATCTGAC 
AAAGAAATCG TTGCTGAAGC AGAAAGACTG GATGTAAAAG CCATGGGCCC TCTTGTTCTA 
ACTGAAGTTC TTTTTAATGA GAAGATTAGA GAACAGATTA AGAAATACAG GCGCCATTTC 
CTACGATTTT GTCACAACAA CAAAAAAGCC CAACGGTACC TTCTTCATGG TTTGGAGTGT 
GTGGTAGCAA TGCATCAAGC TCAGCTTATC TCCAAGATTC CACATATCTT GAAGGAGATG 
TACGATGCAG ACCTTTTAGA AGAAGAGGTC ATCATCAGCT GGTCGGAAAA GGCCTCTAAG 
AAATATGTCT CCAAAGAACT TGCCAAAGAG ATTCGTGTCA AAGCAGAACC ATTTATAAAA 
TGGTTGAAGG AGGCAGAGGA AGAATCTTCT GGTGGCGAAG AAGAAGATGA AGATGAGAAC 
ATTGAGGTGG TGTATTCGAA GGCTGCCAGT GTACCGAAAG TTGAGACTGT AAAGTCAGAC 
AACAAGGATG ACGACATCGA TATTGATGCC ATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_892116.2
CDS	534..1829
Translation	MSVNVNRSVSDQFYRYKMPRLIAKVEGKGNGIKTVIVNMVDVAKALNRPPTYPTKYFGCELGAQTQFDVKNDRYIVNGSHEANKLQDMLDGFIKKFVLCPECENPETDLHVNPKKQTIGNSCKACGYRGMLDTHHKLCTFILKNPPENSDSGTGKKEKEKKNRKGKDKENGSVSSSETPPPPPPPNEINPPPHTMEEEEDDDWGEDTTEEAQRRRMDEISDHAKVLTLSDDLERTIEERVNILFDFVKKKKEEGVIDSSDKEIVAEAERLDVKAMGPLVLTEVLFNEKIREQIKKYRRHFLRFCHNNKKAQRYLLHGLECVVAMHQAQLISKIPHILKEMYDADLLEEEVIISWSEKASKKYVSKELAKEIRVKAEPFIKWLKEAEEESSGGEEEDEDENIEVVYSKAASVPKVETVKSDNKDDDIDIDAI

Target ORF information:

RefSeq Version	NM_183004.4
Organism	Homo sapiens(human)
Definition	Homo sapiens eukaryotic translation initiation factor 5 (EIF5), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_183004.4

ORF Insert Sequence:

ATGTCTGTCA ATGTCAACCG CAGCGTGTCA GACCAGTTCT ATCGCTACAA GATGCCCCGT 
CTGATTGCCA AGGTTGAGGG CAAAGGCAAT GGAATCAAGA CAGTTATAGT CAACATGGTT 
GACGTTGCAA AGGCGCTTAA TCGGCCTCCA ACGTATCCCA CCAAATATTT TGGTTGTGAG 
CTGGGAGCAC AGACCCAGTT TGATGTTAAG AATGACCGTT ACATTGTCAA TGGATCTCAT 
GAGGCGAATA AGCTGCAAGA CATGTTGGAT GGATTCATTA AAAAATTTGT TCTCTGTCCT 
GAATGTGAGA ATCCTGAAAC AGATTTGCAT GTCAATCCAA AGAAGCAAAC AATAGGTAAT 
TCTTGTAAAG CCTGTGGCTA TCGAGGCATG CTTGACACAC ATCATAAACT CTGCACATTC 
ATTCTCAAAA ACCCACCTGA GAATAGTGAC AGTGGTACAG GAAAGAAAGA AAAAGAAAAG 
AAAAACAGAA AGGGCAAAGA CAAGGAAAAT GGCTCCGTAT CCAGCAGTGA GACACCACCA 
CCACCACCAC CACCAAATGA AATTAATCCT CCTCCACATA CAATGGAAGA AGAGGAGGAT 
GATGACTGGG GAGAAGATAC AACTGAGGAA GCTCAAAGGC GTCGAATGGA TGAAATCAGT 
GACCATGCAA AAGTTCTGAC ACTCAGTGAT GATTTGGAAA GAACAATTGA GGAGAGGGTC 
AATATCCTCT TTGATTTTGT TAAGAAAAAG AAAGAAGAGG GTGTTATTGA TTCATCTGAC 
AAAGAAATCG TTGCTGAAGC AGAAAGACTG GATGTAAAAG CCATGGGCCC TCTTGTTCTA 
ACTGAAGTTC TTTTTAATGA GAAGATTAGA GAACAGATTA AGAAATACAG GCGCCATTTC 
CTACGATTTT GTCACAACAA CAAAAAAGCC CAACGGTACC TTCTTCATGG TTTGGAGTGT 
GTGGTAGCAA TGCATCAAGC TCAGCTTATC TCCAAGATTC CACATATCTT GAAGGAGATG 
TACGATGCAG ACCTTTTAGA AGAAGAGGTC ATCATCAGCT GGTCGGAAAA GGCCTCTAAG 
AAATATGTCT CCAAAGAACT TGCCAAAGAG ATTCGTGTCA AAGCAGAACC ATTTATAAAA 
TGGTTGAAGG AGGCAGAGGA AGAATCTTCT GGTGGCGAAG AAGAAGATGA AGATGAGAAC 
ATTGAGGTGG TGTATTCGAA GGCTGCCAGT GTACCGAAAG TTGAGACTGT AAAGTCAGAC 
AACAAGGATG ACGACATCGA TATTGATGCC ATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EIF5 ( NM_001969.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_001960.2 Homo sapiens eukaryotic translation initiation factor 5 (EIF5), transcript variant 1, mRNA.

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CloneID	OHu12534
Clone ID Related Accession (Same CDS sequence)	NM_001969.4 , NM_183004.5 , NM_183004.4 , NM_001969.5
Accession Version	NM_001969.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1296bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-07
Organism	Homo sapiens(human)
Product	eukaryotic translation initiation factor 5
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL138976.5. On Nov 23, 2018 this sequence version replaced NM_001969.4. Transcript Variant: This variant (1) represents the longer transcript. Variants 1 and 2 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.83710.1, SRR1660803.200837.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000216554.8/ ENSP00000216554.3 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGTCTGTCA ATGTCAACCG CAGCGTGTCA GACCAGTTCT ATCGCTACAA GATGCCCCGT 
CTGATTGCCA AGGTTGAGGG CAAAGGCAAT GGAATCAAGA CAGTTATAGT CAACATGGTT 
GACGTTGCAA AGGCGCTTAA TCGGCCTCCA ACGTATCCCA CCAAATATTT TGGTTGTGAG 
CTGGGAGCAC AGACCCAGTT TGATGTTAAG AATGACCGTT ACATTGTCAA TGGATCTCAT 
GAGGCGAATA AGCTGCAAGA CATGTTGGAT GGATTCATTA AAAAATTTGT TCTCTGTCCT 
GAATGTGAGA ATCCTGAAAC AGATTTGCAT GTCAATCCAA AGAAGCAAAC AATAGGTAAT 
TCTTGTAAAG CCTGTGGCTA TCGAGGCATG CTTGACACAC ATCATAAACT CTGCACATTC 
ATTCTCAAAA ACCCACCTGA GAATAGTGAC AGTGGTACAG GAAAGAAAGA AAAAGAAAAG 
AAAAACAGAA AGGGCAAAGA CAAGGAAAAT GGCTCCGTAT CCAGCAGTGA GACACCACCA 
CCACCACCAC CACCAAATGA AATTAATCCT CCTCCACATA CAATGGAAGA AGAGGAGGAT 
GATGACTGGG GAGAAGATAC AACTGAGGAA GCTCAAAGGC GTCGAATGGA TGAAATCAGT 
GACCATGCAA AAGTTCTGAC ACTCAGTGAT GATTTGGAAA GAACAATTGA GGAGAGGGTC 
AATATCCTCT TTGATTTTGT TAAGAAAAAG AAAGAAGAGG GTGTTATTGA TTCATCTGAC 
AAAGAAATCG TTGCTGAAGC AGAAAGACTG GATGTAAAAG CCATGGGCCC TCTTGTTCTA 
ACTGAAGTTC TTTTTAATGA GAAGATTAGA GAACAGATTA AGAAATACAG GCGCCATTTC 
CTACGATTTT GTCACAACAA CAAAAAAGCC CAACGGTACC TTCTTCATGG TTTGGAGTGT 
GTGGTAGCAA TGCATCAAGC TCAGCTTATC TCCAAGATTC CACATATCTT GAAGGAGATG 
TACGATGCAG ACCTTTTAGA AGAAGAGGTC ATCATCAGCT GGTCGGAAAA GGCCTCTAAG 
AAATATGTCT CCAAAGAACT TGCCAAAGAG ATTCGTGTCA AAGCAGAACC ATTTATAAAA 
TGGTTGAAGG AGGCAGAGGA AGAATCTTCT GGTGGCGAAG AAGAAGATGA AGATGAGAAC 
ATTGAGGTGG TGTATTCGAA GGCTGCCAGT GTACCGAAAG TTGAGACTGT AAAGTCAGAC 
AACAAGGATG ACGACATCGA TATTGATGCC ATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001960.2
CDS	442..1737
Translation	MSVNVNRSVSDQFYRYKMPRLIAKVEGKGNGIKTVIVNMVDVAKALNRPPTYPTKYFGCELGAQTQFDVKNDRYIVNGSHEANKLQDMLDGFIKKFVLCPECENPETDLHVNPKKQTIGNSCKACGYRGMLDTHHKLCTFILKNPPENSDSGTGKKEKEKKNRKGKDKENGSVSSSETPPPPPPPNEINPPPHTMEEEEDDDWGEDTTEEAQRRRMDEISDHAKVLTLSDDLERTIEERVNILFDFVKKKKEEGVIDSSDKEIVAEAERLDVKAMGPLVLTEVLFNEKIREQIKKYRRHFLRFCHNNKKAQRYLLHGLECVVAMHQAQLISKIPHILKEMYDADLLEEEVIISWSEKASKKYVSKELAKEIRVKAEPFIKWLKEAEEESSGGEEEDEDENIEVVYSKAASVPKVETVKSDNKDDDIDIDAI

Target ORF information:

RefSeq Version	NM_001969.5
Organism	Homo sapiens(human)
Definition	Homo sapiens eukaryotic translation initiation factor 5 (EIF5), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001969.5

ORF Insert Sequence:

ATGTCTGTCA ATGTCAACCG CAGCGTGTCA GACCAGTTCT ATCGCTACAA GATGCCCCGT 
CTGATTGCCA AGGTTGAGGG CAAAGGCAAT GGAATCAAGA CAGTTATAGT CAACATGGTT 
GACGTTGCAA AGGCGCTTAA TCGGCCTCCA ACGTATCCCA CCAAATATTT TGGTTGTGAG 
CTGGGAGCAC AGACCCAGTT TGATGTTAAG AATGACCGTT ACATTGTCAA TGGATCTCAT 
GAGGCGAATA AGCTGCAAGA CATGTTGGAT GGATTCATTA AAAAATTTGT TCTCTGTCCT 
GAATGTGAGA ATCCTGAAAC AGATTTGCAT GTCAATCCAA AGAAGCAAAC AATAGGTAAT 
TCTTGTAAAG CCTGTGGCTA TCGAGGCATG CTTGACACAC ATCATAAACT CTGCACATTC 
ATTCTCAAAA ACCCACCTGA GAATAGTGAC AGTGGTACAG GAAAGAAAGA AAAAGAAAAG 
AAAAACAGAA AGGGCAAAGA CAAGGAAAAT GGCTCCGTAT CCAGCAGTGA GACACCACCA 
CCACCACCAC CACCAAATGA AATTAATCCT CCTCCACATA CAATGGAAGA AGAGGAGGAT 
GATGACTGGG GAGAAGATAC AACTGAGGAA GCTCAAAGGC GTCGAATGGA TGAAATCAGT 
GACCATGCAA AAGTTCTGAC ACTCAGTGAT GATTTGGAAA GAACAATTGA GGAGAGGGTC 
AATATCCTCT TTGATTTTGT TAAGAAAAAG AAAGAAGAGG GTGTTATTGA TTCATCTGAC 
AAAGAAATCG TTGCTGAAGC AGAAAGACTG GATGTAAAAG CCATGGGCCC TCTTGTTCTA 
ACTGAAGTTC TTTTTAATGA GAAGATTAGA GAACAGATTA AGAAATACAG GCGCCATTTC 
CTACGATTTT GTCACAACAA CAAAAAAGCC CAACGGTACC TTCTTCATGG TTTGGAGTGT 
GTGGTAGCAA TGCATCAAGC TCAGCTTATC TCCAAGATTC CACATATCTT GAAGGAGATG 
TACGATGCAG ACCTTTTAGA AGAAGAGGTC ATCATCAGCT GGTCGGAAAA GGCCTCTAAG 
AAATATGTCT CCAAAGAACT TGCCAAAGAG ATTCGTGTCA AAGCAGAACC ATTTATAAAA 
TGGTTGAAGG AGGCAGAGGA AGAATCTTCT GGTGGCGAAG AAGAAGATGA AGATGAGAAC 
ATTGAGGTGG TGTATTCGAA GGCTGCCAGT GTACCGAAAG TTGAGACTGT AAAGTCAGAC 
AACAAGGATG ACGACATCGA TATTGATGCC ATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Competition between translation initiation factor eIF5 and its mimic protein 5MP determines non-AUG initiation rate genome-wide.
Nucleic acids research45(20)11941-11953(2017 Nov)
Tang L,Morris J,Wan J,Moore C,Fujita Y,Gillaspie S,Aube E,Nanda J,Marques M,Jangal M,Anderson A,Cox C,Hiraishi H,Dong L,Saito H,Singh CR,Witcher M,Topisirovic I,Qian SB,Asano K

A Case-Matched Gender Comparison Transcriptomic Screen Identifies eIF4E and eIF5 as Potential Prognostic Markers in Male Breast Cancer.
Clinical cancer research : an official journal of the American Association for Cancer Research23(10)2575-2583(2017 May)
Humphries MP,Sundara Rajan S,Droop A,Suleman CAB,Carbone C,Nilsson C,Honarpisheh H,Cserni G,Dent J,Fulford L,Jordan LB,Jones JL,Kanthan R,Litwiniuk M,Di Benedetto A,Mottolese M,Provenzano E,Shousha S,Stephens M,Walker RA,Kulka J,Ellis IO,Jeffery M,Thygesen HH,Cappelletti V,Daidone MG,Hedenfalk IA,Fj?llskog ML,Melisi D,Stead LF,Shaaban AM,Speirs V

Defect in the GTPase activating protein (GAP) function of eIF5 causes repression of GCN4 translation.
Biochemical and biophysical research communications486(4)1110-1115(2017 05)
Antony A C,Alone PV

Overexpression of eIF5 or its protein mimic 5MP perturbs eIF2 function and induces ATF4 translation through delayed re-initiation.
Nucleic acids research44(18)8704-8713(2016 Oct)
Kozel C,Thompson B,Hustak S,Moore C,Nakashima A,Singh CR,Reid M,Cox C,Papadopoulos E,Luna RE,Anderson A,Tagami H,Hiraishi H,Slone EA,Yoshino KI,Asano M,Gillaspie S,Nietfeld J,Perchellet JP,Rothenburg S,Masai H,Wagner G,Beeser A,Kikkawa U,Fleming SD,Asano K

Sliding of a 43S ribosomal complex from the recognized AUG codon triggered by a delay in eIF2-bound GTP hydrolysis.
Nucleic acids research44(4)1882-93(2016 Feb)
Terenin IM,Akulich KA,Andreev DE,Polyanskaya SA,Shatsky IN,Dmitriev SE

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EIF5 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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