EIF4G1 gene cDNA ORF clone, Homo sapiens(human)

mRNA	Protein	Name
NM_198241.2	NP_937884.1	eukaryotic translation initiation factor 4 gamma 1 isoform 5
NM_198242.2	NP_937885.1	eukaryotic translation initiation factor 4 gamma 1 isoform 3
NM_001291157.1	NP_001278086.1	eukaryotic translation initiation factor 4 gamma 1 isoform 7
NM_001291157.2	NP_001278086.2	eukaryotic translation initiation factor 4 gamma 1 isoform 7
NM_001194946.1	NP_001181875.1	eukaryotic translation initiation factor 4 gamma 1 isoform 6
NM_198244.2	NP_937887.1	eukaryotic translation initiation factor 4 gamma 1 isoform 2
NM_182917.4	NP_886553.3	eukaryotic translation initiation factor 4 gamma 1 isoform 1
NM_198241.3	NP_937884.2	eukaryotic translation initiation factor 4 gamma 1 isoform 5
NM_004953.4	NP_004944.3	eukaryotic translation initiation factor 4 gamma 1 isoform 4
NM_004953.5	NP_004944.3	eukaryotic translation initiation factor 4 gamma 1 isoform 4
NM_198242.3	NP_937885.1	eukaryotic translation initiation factor 4 gamma 1 isoform 3
NM_198244.3	NP_937887.2	eukaryotic translation initiation factor 4 gamma 1 isoform 2
NM_001194947.1	NP_001181876.1	eukaryotic translation initiation factor 4 gamma 1 isoform 6
NM_001194946.2	NP_001181875.2	eukaryotic translation initiation factor 4 gamma 1 isoform 6

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu13769	NM_182917.4 Latest version!	Homo sapiens eukaryotic translation initiation factor 4 gamma 1 (EIF4G1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$659.50-$923.30 $1319.00
OHu13773	NM_198242.3 Latest version!	Homo sapiens eukaryotic translation initiation factor 4 gamma 1 (EIF4G1), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$274.50-$384.30 $549.00
OHu13775	NM_001194946.1 Latest version!	Homo sapiens eukaryotic translation initiation factor 4 gamma 1 (EIF4G1), transcript variant 6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$274.50-$384.30 $549.00
OHu13775	NM_001194947.1 Latest version!	Homo sapiens eukaryotic translation initiation factor 4 gamma 1 (EIF4G1), transcript variant 7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$274.50-$384.30 $549.00
OHu13776	NM_004953.5 Latest version!	Homo sapiens eukaryotic translation initiation factor 4 gamma 1 (EIF4G1), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$274.50-$384.30 $549.00
OHu18102	NM_198241.2 Latest version!	Homo sapiens eukaryotic translation initiation factor 4 gamma 1 (EIF4G1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$659.50-$923.30 $1319.00
OHu108646	NM_001194946.2 Latest version!	Homo sapiens eukaryotic translation initiation factor 4 gamma 1 (EIF4G1), transcript variant 6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OHu108651	NM_198241.3 Latest version!	Homo sapiens eukaryotic translation initiation factor 4 gamma 1 (EIF4G1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OHu108653	NM_001291157.2 Latest version!	Homo sapiens eukaryotic translation initiation factor 4 gamma 1 (EIF4G1), transcript variant 8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OHu108656	NM_198244.3 Latest version!	Homo sapiens eukaryotic translation initiation factor 4 gamma 1 (EIF4G1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OHu13778	NM_198244.2 Latest version!	Homo sapiens eukaryotic translation initiation factor 4 gamma 1 (EIF4G1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$384.30 $549.00
OHu15300	NM_001291157.1 Latest version!	Homo sapiens eukaryotic translation initiation factor 4 gamma 1 (EIF4G1), transcript variant 8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
View Old Accession Versions >>
OHu13776	NM_004953.4	Homo sapiens eukaryotic translation initiation factor 4 gamma 1 (EIF4G1), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$274.50-$384.30 $549.00
OHu13773	NM_198242.2	Homo sapiens eukaryotic translation initiation factor 4 gamma 1 (EIF4G1), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$274.50-$384.30 $549.00
Hide Old Accession Versions >>

EIF4G1 ( NM_182917.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_886553.3 Homo sapiens eukaryotic translation initiation factor 4 gamma 1 (EIF4G1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu13769
Clone ID Related Accession (Same CDS sequence)	NM_182917.4
Accession Version	NM_182917.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4803bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-20
Organism	Homo sapiens(human)
Product	eukaryotic translation initiation factor 4 gamma 1 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC347118.1, AY082886.1, AF002815.1, AK308319.1 and BQ011010.1. On Aug 13, 2010 this sequence version replaced NM_182917.3. Transcript Variant: This variant (1) represents the longest transcript and encodes isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF281070.1, AY082886.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## regulatory uORF :: PMID: 12052860 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGAACAAAG CTCCACAGTC CACAGGCCCC CCACCCGCCC CATCCCCCGG ACTCCCACAG 
CCAGCGTTTC CCCCGGGGCA GACAGCGCCG GTGGTGTTCA GTACGCCACA AGCGACACAA 
ATGAACACGC CTTCTCAGCC CCGCCAGCAC TTCTACCCTA GCCGGGCCCA GCCCCCGAGC 
AGTGCAGCCT CCCGAGTGCA GAGTGCAGCC CCTGCCCGCC CTGGCCCAGC TGCCCATGTC 
TACCCTGCTG GATCCCAAGT AATGATGATC CCTTCCCAGA TCTCCTACCC AGCCTCCCAG 
GGGGCCTACT ACATCCCTGG ACAGGGGCGT TCCACATACG TTGTCCCGAC ACAGCAGTAC 
CCTGTGCAGC CAGGAGCCCC AGGCTTCTAT CCAGGTGCAA GCCCTACAGA ATTTGGGACC 
TACGCTGGCG CCTACTATCC AGCCCAAGGG GTGCAGCAGT TTCCCACTGG CGTGGCCCCC 
GCCCCAGTTT TGATGAACCA GCCACCCCAG ATTGCTCCCA AGAGGGAGCG TAAGACGATC 
CGAATTCGAG ATCCAAACCA AGGAGGAAAG GATATCACAG AGGAGATCAT GTCTGGGGCC 
CGCACTGCCT CCACACCCAC CCCTCCCCAG ACGGGAGGCG GTCTGGAGCC TCAAGCTAAT 
GGGGAGACGC CCCAGGTTGC TGTCATTGTC CGGCCAGATG ACCGGTCACA GGGAGCAATC 
ATTGCTGACC GGCCAGGGCT GCCTGGCCCA GAGCATAGCC CTTCAGAATC CCAGCCTTCG 
TCGCCTTCTC CGACCCCATC ACCATCCCCA GTCTTGGAAC CGGGGTCTGA GCCTAATCTC 
GCAGTCCTCT CTATTCCTGG GGACACTATG ACAACTATAC AAATGTCTGT AGAAGAATCA 
ACCCCCATCT CCCGTGAAAC TGGGGAGCCA TATCGCCTCT CTCCAGAACC CACTCCTCTC 
GCCGAACCCA TACTGGAAGT AGAAGTGACA CTTAGCAAAC CGGTTCCAGA ATCTGAGTTT 
TCTTCCAGTC CTCTCCAGGC TCCCACCCCT TTGGCATCTC ACACAGTGGA AATTCATGAG 
CCTAATGGCA TGGTCCCATC TGAAGATCTG GAACCAGAGG TGGAGTCAAG CCCAGAGCTT 
GCTCCTCCCC CAGCTTGCCC CTCCGAATCC CCTGTGCCCA TTGCTCCAAC TGCCCAACCT 
GAGGAACTGC TCAACGGAGC CCCCTCGCCA CCAGCTGTGG ACTTAAGCCC AGTCAGTGAG 
CCAGAGGAGC AGGCCAAGGA GGTGACAGCA TCAATGGCGC CCCCCACCAT CCCCTCTGCT 
ACTCCAGCTA CGGCTCCTTC AGCTACTTCC CCAGCTCAGG AGGAGGAAAT GGAAGAAGAA 
GAAGAAGAGG AAGAAGGAGA AGCAGGAGAA GCAGGAGAAG CTGAGAGTGA GAAAGGAGGA 
GAGGAACTGC TCCCCCCAGA GAGTACCCCT ATTCCAGCCA ACTTGTCTCA GAATTTGGAG 
GCAGCAGCAG CCACTCAAGT GGCAGTATCT GTGCCAAAGA GGAGACGGAA AATTAAGGAG 
CTAAATAAGA AGGAGGCTGT TGGAGACCTT CTGGATGCCT TCAAGGAGGC GAACCCGGCA 
GTACCAGAGG TGGAAAATCA GCCTCCTGCA GGCAGCAATC CAGGCCCAGA GTCTGAGGGC 
AGTGGTGTGC CCCCACGTCC TGAGGAAGCA GATGAGACCT GGGACTCAAA GGAAGACAAA 
ATTCACAATG CTGAGAACAT CCAGCCCGGG GAACAGAAGT ATGAATATAA GTCAGATCAG 
TGGAAGCCTC TAAACCTAGA GGAGAAAAAA CGTTACGACC GTGAGTTCCT GCTTGGTTTT 
CAGTTCATCT TTGCCAGTAT GCAGAAGCCA GAGGGATTGC CACATATCAG TGACGTGGTG 
CTGGACAAGG CCAATAAAAC ACCACTGCGG CCACTGGATC CCACTAGACT ACAAGGCATA 
AATTGTGGCC CAGACTTCAC TCCATCCTTT GCCAACCTTG GCCGGACAAC CCTTAGCACC 
CGTGGGCCCC CAAGGGGTGG GCCAGGTGGG GAGCTGCCCC GTGGGCCGCA GGCTGGCCTG 
GGACCCCGGC GCTCTCAGCA GGGACCCCGA AAAGAACCAC GCAAGATCAT TGCCACAGTG 
TTAATGACCG AAGATATAAA ACTGAACAAA GCAGAGAAAG CCTGGAAACC CAGCAGCAAG 
CGGACGGCGG CTGATAAGGA TCGAGGGGAA GAAGATGCTG ATGGCAGCAA AACCCAGGAC 
CTATTCCGCA GGGTGCGCTC CATCCTGAAT AAACTGACAC CCCAGATGTT CCAGCAGCTG 
ATGAAGCAAG TGACGCAGCT GGCCATCGAC ACCGAGGAAC GCCTCAAAGG GGTCATTGAC 
CTCATTTTTG AGAAGGCCAT TTCAGAGCCC AACTTCTCTG TGGCCTATGC CAACATGTGC 
CGCTGCCTCA TGGCGCTGAA AGTGCCCACT ACGGAAAAGC CAACAGTGAC TGTGAACTTC 
CGAAAGCTGT TGTTGAATCG ATGTCAGAAG GAGTTTGAGA AAGACAAAGA TGATGATGAG 
GTTTTTGAGA AGAAGCAAAA AGAGATGGAT GAAGCTGCTA CGGCAGAGGA ACGAGGACGC 
CTGAAGGAAG AGCTGGAAGA GGCTCGGGAC ATAGCCCGGC GGCGCTCTTT AGGGAATATC 
AAGTTTATTG GAGAGTTGTT CAAACTGAAG ATGTTAACAG AGGCAATAAT GCATGACTGT 
GTGGTCAAAC TGCTTAAGAA CCATGATGAA GAGTCCCTTG AGTGCCTTTG TCGTCTGCTC 
ACCACCATTG GCAAAGACCT GGACTTTGAA AAAGCCAAGC CCCGAATGGA TCAGTATTTC 
AACCAGATGG AAAAAATCAT TAAAGAAAAG AAGACGTCAT CCCGCATCCG CTTTATGCTG 
CAGGACGTGC TGGATCTGCG AGGGAGCAAT TGGGTGCCAC GCCGAGGGGA TCAGGGTCCC 
AAGACCATTG ACCAGATCCA TAAGGAGGCT GAGATGGAAG AACATCGAGA GCACATCAAA 
GTGCAGCAGC TCATGGCCAA GGGCAGTGAC AAGCGTCGGG GCGGTCCTCC AGGCCCTCCC 
ATCAGCCGTG GACTTCCCCT TGTGGATGAT GGTGGCTGGA ACACAGTTCC CATCAGCAAA 
GGTAGCCGCC CCATTGACAC CTCACGACTC ACCAAGATCA CCAAGCCTGG CTCCATCGAT 
TCTAACAACC AGCTCTTTGC ACCTGGAGGG CGACTGAGCT GGGGCAAGGG CAGCAGCGGA 
GGCTCAGGAG CCAAGCCCTC AGACGCAGCA TCAGAAGCTG CTCGCCCAGC TACTAGTACT 
TTGAATCGCT TCTCAGCCCT TCAACAAGCG GTACCCACAG AAAGCACAGA TAATAGACGT 
GTGGTGCAGA GGAGTAGCTT GAGCCGAGAA CGAGGCGAGA AAGCTGGAGA CCGAGGAGAC 
CGCCTAGAGC GGAGTGAACG GGGAGGGGAC CGTGGGGACC GGCTTGATCG TGCGCGGACA 
CCTGCTACCA AGCGGAGCTT CAGCAAGGAA GTGGAGGAGC GGAGTAGAGA ACGGCCCTCC 
CAGCCTGAGG GGCTGCGCAA GGCAGCTAGC CTCACGGAGG ATCGGGACCG TGGGCGGGAT 
GCCGTGAAGC GAGAAGCTGC CCTACCCCCA GTGAGCCCCC TGAAGGCGGC TCTCTCTGAG 
GAGGAGTTAG AGAAGAAATC CAAGGCTATC ATTGAGGAAT ATCTCCATCT CAATGACATG 
AAAGAGGCAG TCCAGTGCGT GCAGGAGCTG GCCTCACCCT CCTTGCTCTT CATCTTTGTA 
CGGCATGGTG TCGAGTCTAC GCTGGAGCGC AGTGCCATTG CTCGTGAGCA TATGGGGCAG 
CTGCTGCACC AGCTGCTCTG TGCTGGGCAT CTGTCTACTG CTCAGTACTA CCAAGGGTTG 
TATGAAATCT TGGAATTGGC TGAGGACATG GAAATTGACA TCCCCCACGT GTGGCTCTAC 
CTAGCGGAAC TGGTAACACC CATTCTGCAG GAAGGTGGGG TGCCCATGGG GGAGCTGTTC 
AGGGAGATTA CAAAGCCTCT GAGACCGTTG GGCAAAGCTG CTTCCCTGTT GCTGGAGATC 
CTGGGCCTCC TGTGCAAAAG CATGGGTCCT AAAAAGGTGG GGACGCTGTG GCGAGAAGCC 
GGGCTTAGCT GGAAGGAATT TCTACCTGAA GGCCAGGACA TTGGTGCATT CGTCGCTGAA 
CAGAAGGTGG AGTATACCCT GGGAGAGGAG TCGGAAGCCC CTGGCCAGAG GGCACTCCCC 
TCCGAGGAGC TGAACAGGCA GCTGGAGAAG CTGCTGAAGG AGGGCAGCAG TAACCAGCGG 
GTGTTCGACT GGATAGAGGC CAACCTGAGT GAGCAGCAGA TAGTATCCAA CACGTTAGTT 
CGAGCCCTCA TGACGGCTGT CTGCTATTCT GCAATTATTT TTGAGACTCC CCTCCGAGTG 
GACGTTGCAG TGCTGAAAGC GCGAGCGAAG CTGCTGCAGA AATACCTGTG TGACGAGCAG 
AAGGAGCTAC AGGCGCTCTA CGCCCTCCAG GCCCTTGTAG TGACCTTAGA ACAGCCTCCC 
AACCTGCTGC GGATGTTCTT TGACGCACTG TATGACGAGG ACGTGGTGAA GGAGGATGCC 
TTCTACAGTT GGGAGAGTAG CAAGGACCCC GCTGAGCAGC AGGGCAAGGG TGTGGCCCTT 
AAATCTGTCA CAGCCTTCTT CAAGTGGCTC CGTGAAGCAG AGGAGGAGTC TGACCACAAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_886553.3
CDS	417..5219
Translation	MNKAPQSTGPPPAPSPGLPQPAFPPGQTAPVVFSTPQATQMNTPSQPRQHFYPSRAQPPSSAASRVQSAAPARPGPAAHVYPAGSQVMMIPSQISYPASQGAYYIPGQGRSTYVVPTQQYPVQPGAPGFYPGASPTEFGTYAGAYYPAQGVQQFPTGVAPAPVLMNQPPQIAPKRERKTIRIRDPNQGGKDITEEIMSGARTASTPTPPQTGGGLEPQANGETPQVAVIVRPDDRSQGAIIADRPGLPGPEHSPSESQPSSPSPTPSPSPVLEPGSEPNLAVLSIPGDTMTTIQMSVEESTPISRETGEPYRLSPEPTPLAEPILEVEVTLSKPVPESEFSSSPLQAPTPLASHTVEIHEPNGMVPSEDLEPEVESSPELAPPPACPSESPVPIAPTAQPEELLNGAPSPPAVDLSPVSEPEEQAKEVTASMAPPTIPSATPATAPSATSPAQEEEMEEEEEEEEGEAGEAGEAESEKGGEELLPPESTPIPANLSQNLEAAAATQVAVSVPKRRRKIKELNKKEAVGDLLDAFKEANPAVPEVENQPPAGSNPGPESEGSGVPPRPEEADETWDSKEDKIHNAENIQPGEQKYEYKSDQWKPLNLEEKKRYDREFLLGFQFIFASMQKPEGLPHISDVVLDKANKTPLRPLDPTRLQGINCGPDFTPSFANLGRTTLSTRGPPRGGPGGELPRGPQAGLGPRRSQQGPRKEPRKIIATVLMTEDIKLNKAEKAWKPSSKRTAADKDRGEEDADGSKTQDLFRRVRSILNKLTPQMFQQLMKQVTQLAIDTEERLKGVIDLIFEKAISEPNFSVAYANMCRCLMALKVPTTEKPTVTVNFRKLLLNRCQKEFEKDKDDDEVFEKKQKEMDEAATAEERGRLKEELEEARDIARRRSLGNIKFIGELFKLKMLTEAIMHDCVVKLLKNHDEESLECLCRLLTTIGKDLDFEKAKPRMDQYFNQMEKIIKEKKTSSRIRFMLQDVLDLRGSNWVPRRGDQGPKTIDQIHKEAEMEEHREHIKVQQLMAKGSDKRRGGPPGPPISRGLPLVDDGGWNTVPISKGSRPIDTSRLTKITKPGSIDSNNQLFAPGGRLSWGKGSSGGSGAKPSDAASEAARPATSTLNRFSALQQAVPTESTDNRRVVQRSSLSRERGEKAGDRGDRLERSERGGDRGDRLDRARTPATKRSFSKEVEERSRERPSQPEGLRKAASLTEDRDRGRDAVKREAALPPVSPLKAALSEEELEKKSKAIIEEYLHLNDMKEAVQCVQELASPSLLFIFVRHGVESTLERSAIAREHMGQLLHQLLCAGHLSTAQYYQGLYEILELAEDMEIDIPHVWLYLAELVTPILQEGGVPMGELFREITKPLRPLGKAASLLLEILGLLCKSMGPKKVGTLWREAGLSWKEFLPEGQDIGAFVAEQKVEYTLGEESEAPGQRALPSEELNRQLEKLLKEGSSNQRVFDWIEANLSEQQIVSNTLVRALMTAVCYSAIIFETPLRVDVAVLKARAKLLQKYLCDEQKELQALYALQALVVTLEQPPNLLRMFFDALYDEDVVKEDAFYSWESSKDPAEQQGKGVALKSVTAFFKWLREAEEESDHN

Target ORF information:

RefSeq Version	NM_182917.4
Organism	Homo sapiens(human)
Definition	Homo sapiens eukaryotic translation initiation factor 4 gamma 1 (EIF4G1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_182917.4

ORF Insert Sequence:

ATGAACAAAG CTCCACAGTC CACAGGCCCC CCACCCGCCC CATCCCCCGG ACTCCCACAG 
CCAGCGTTTC CCCCGGGGCA GACAGCGCCG GTGGTGTTCA GTACGCCACA AGCGACACAA 
ATGAACACGC CTTCTCAGCC CCGCCAGCAC TTCTACCCTA GCCGGGCCCA GCCCCCGAGC 
AGTGCAGCCT CCCGAGTGCA GAGTGCAGCC CCTGCCCGCC CTGGCCCAGC TGCCCATGTC 
TACCCTGCTG GATCCCAAGT AATGATGATC CCTTCCCAGA TCTCCTACCC AGCCTCCCAG 
GGGGCCTACT ACATCCCTGG ACAGGGGCGT TCCACATACG TTGTCCCGAC ACAGCAGTAC 
CCTGTGCAGC CAGGAGCCCC AGGCTTCTAT CCAGGTGCAA GCCCTACAGA ATTTGGGACC 
TACGCTGGCG CCTACTATCC AGCCCAAGGG GTGCAGCAGT TTCCCACTGG CGTGGCCCCC 
GCCCCAGTTT TGATGAACCA GCCACCCCAG ATTGCTCCCA AGAGGGAGCG TAAGACGATC 
CGAATTCGAG ATCCAAACCA AGGAGGAAAG GATATCACAG AGGAGATCAT GTCTGGGGCC 
CGCACTGCCT CCACACCCAC CCCTCCCCAG ACGGGAGGCG GTCTGGAGCC TCAAGCTAAT 
GGGGAGACGC CCCAGGTTGC TGTCATTGTC CGGCCAGATG ACCGGTCACA GGGAGCAATC 
ATTGCTGACC GGCCAGGGCT GCCTGGCCCA GAGCATAGCC CTTCAGAATC CCAGCCTTCG 
TCGCCTTCTC CGACCCCATC ACCATCCCCA GTCTTGGAAC CGGGGTCTGA GCCTAATCTC 
GCAGTCCTCT CTATTCCTGG GGACACTATG ACAACTATAC AAATGTCTGT AGAAGAATCA 
ACCCCCATCT CCCGTGAAAC TGGGGAGCCA TATCGCCTCT CTCCAGAACC CACTCCTCTC 
GCCGAACCCA TACTGGAAGT AGAAGTGACA CTTAGCAAAC CGGTTCCAGA ATCTGAGTTT 
TCTTCCAGTC CTCTCCAGGC TCCCACCCCT TTGGCATCTC ACACAGTGGA AATTCATGAG 
CCTAATGGCA TGGTCCCATC TGAAGATCTG GAACCAGAGG TGGAGTCAAG CCCAGAGCTT 
GCTCCTCCCC CAGCTTGCCC CTCCGAATCC CCTGTGCCCA TTGCTCCAAC TGCCCAACCT 
GAGGAACTGC TCAACGGAGC CCCCTCGCCA CCAGCTGTGG ACTTAAGCCC AGTCAGTGAG 
CCAGAGGAGC AGGCCAAGGA GGTGACAGCA TCAATGGCGC CCCCCACCAT CCCCTCTGCT 
ACTCCAGCTA CGGCTCCTTC AGCTACTTCC CCAGCTCAGG AGGAGGAAAT GGAAGAAGAA 
GAAGAAGAGG AAGAAGGAGA AGCAGGAGAA GCAGGAGAAG CTGAGAGTGA GAAAGGAGGA 
GAGGAACTGC TCCCCCCAGA GAGTACCCCT ATTCCAGCCA ACTTGTCTCA GAATTTGGAG 
GCAGCAGCAG CCACTCAAGT GGCAGTATCT GTGCCAAAGA GGAGACGGAA AATTAAGGAG 
CTAAATAAGA AGGAGGCTGT TGGAGACCTT CTGGATGCCT TCAAGGAGGC GAACCCGGCA 
GTACCAGAGG TGGAAAATCA GCCTCCTGCA GGCAGCAATC CAGGCCCAGA GTCTGAGGGC 
AGTGGTGTGC CCCCACGTCC TGAGGAAGCA GATGAGACCT GGGACTCAAA GGAAGACAAA 
ATTCACAATG CTGAGAACAT CCAGCCCGGG GAACAGAAGT ATGAATATAA GTCAGATCAG 
TGGAAGCCTC TAAACCTAGA GGAGAAAAAA CGTTACGACC GTGAGTTCCT GCTTGGTTTT 
CAGTTCATCT TTGCCAGTAT GCAGAAGCCA GAGGGATTGC CACATATCAG TGACGTGGTG 
CTGGACAAGG CCAATAAAAC ACCACTGCGG CCACTGGATC CCACTAGACT ACAAGGCATA 
AATTGTGGCC CAGACTTCAC TCCATCCTTT GCCAACCTTG GCCGGACAAC CCTTAGCACC 
CGTGGGCCCC CAAGGGGTGG GCCAGGTGGG GAGCTGCCCC GTGGGCCGCA GGCTGGCCTG 
GGACCCCGGC GCTCTCAGCA GGGACCCCGA AAAGAACCAC GCAAGATCAT TGCCACAGTG 
TTAATGACCG AAGATATAAA ACTGAACAAA GCAGAGAAAG CCTGGAAACC CAGCAGCAAG 
CGGACGGCGG CTGATAAGGA TCGAGGGGAA GAAGATGCTG ATGGCAGCAA AACCCAGGAC 
CTATTCCGCA GGGTGCGCTC CATCCTGAAT AAACTGACAC CCCAGATGTT CCAGCAGCTG 
ATGAAGCAAG TGACGCAGCT GGCCATCGAC ACCGAGGAAC GCCTCAAAGG GGTCATTGAC 
CTCATTTTTG AGAAGGCCAT TTCAGAGCCC AACTTCTCTG TGGCCTATGC CAACATGTGC 
CGCTGCCTCA TGGCGCTGAA AGTGCCCACT ACGGAAAAGC CAACAGTGAC TGTGAACTTC 
CGAAAGCTGT TGTTGAATCG ATGTCAGAAG GAGTTTGAGA AAGACAAAGA TGATGATGAG 
GTTTTTGAGA AGAAGCAAAA AGAGATGGAT GAAGCTGCTA CGGCAGAGGA ACGAGGACGC 
CTGAAGGAAG AGCTGGAAGA GGCTCGGGAC ATAGCCCGGC GGCGCTCTTT AGGGAATATC 
AAGTTTATTG GAGAGTTGTT CAAACTGAAG ATGTTAACAG AGGCAATAAT GCATGACTGT 
GTGGTCAAAC TGCTTAAGAA CCATGATGAA GAGTCCCTTG AGTGCCTTTG TCGTCTGCTC 
ACCACCATTG GCAAAGACCT GGACTTTGAA AAAGCCAAGC CCCGAATGGA TCAGTATTTC 
AACCAGATGG AAAAAATCAT TAAAGAAAAG AAGACGTCAT CCCGCATCCG CTTTATGCTG 
CAGGACGTGC TGGATCTGCG AGGGAGCAAT TGGGTGCCAC GCCGAGGGGA TCAGGGTCCC 
AAGACCATTG ACCAGATCCA TAAGGAGGCT GAGATGGAAG AACATCGAGA GCACATCAAA 
GTGCAGCAGC TCATGGCCAA GGGCAGTGAC AAGCGTCGGG GCGGTCCTCC AGGCCCTCCC 
ATCAGCCGTG GACTTCCCCT TGTGGATGAT GGTGGCTGGA ACACAGTTCC CATCAGCAAA 
GGTAGCCGCC CCATTGACAC CTCACGACTC ACCAAGATCA CCAAGCCTGG CTCCATCGAT 
TCTAACAACC AGCTCTTTGC ACCTGGAGGG CGACTGAGCT GGGGCAAGGG CAGCAGCGGA 
GGCTCAGGAG CCAAGCCCTC AGACGCAGCA TCAGAAGCTG CTCGCCCAGC TACTAGTACT 
TTGAATCGCT TCTCAGCCCT TCAACAAGCG GTACCCACAG AAAGCACAGA TAATAGACGT 
GTGGTGCAGA GGAGTAGCTT GAGCCGAGAA CGAGGCGAGA AAGCTGGAGA CCGAGGAGAC 
CGCCTAGAGC GGAGTGAACG GGGAGGGGAC CGTGGGGACC GGCTTGATCG TGCGCGGACA 
CCTGCTACCA AGCGGAGCTT CAGCAAGGAA GTGGAGGAGC GGAGTAGAGA ACGGCCCTCC 
CAGCCTGAGG GGCTGCGCAA GGCAGCTAGC CTCACGGAGG ATCGGGACCG TGGGCGGGAT 
GCCGTGAAGC GAGAAGCTGC CCTACCCCCA GTGAGCCCCC TGAAGGCGGC TCTCTCTGAG 
GAGGAGTTAG AGAAGAAATC CAAGGCTATC ATTGAGGAAT ATCTCCATCT CAATGACATG 
AAAGAGGCAG TCCAGTGCGT GCAGGAGCTG GCCTCACCCT CCTTGCTCTT CATCTTTGTA 
CGGCATGGTG TCGAGTCTAC GCTGGAGCGC AGTGCCATTG CTCGTGAGCA TATGGGGCAG 
CTGCTGCACC AGCTGCTCTG TGCTGGGCAT CTGTCTACTG CTCAGTACTA CCAAGGGTTG 
TATGAAATCT TGGAATTGGC TGAGGACATG GAAATTGACA TCCCCCACGT GTGGCTCTAC 
CTAGCGGAAC TGGTAACACC CATTCTGCAG GAAGGTGGGG TGCCCATGGG GGAGCTGTTC 
AGGGAGATTA CAAAGCCTCT GAGACCGTTG GGCAAAGCTG CTTCCCTGTT GCTGGAGATC 
CTGGGCCTCC TGTGCAAAAG CATGGGTCCT AAAAAGGTGG GGACGCTGTG GCGAGAAGCC 
GGGCTTAGCT GGAAGGAATT TCTACCTGAA GGCCAGGACA TTGGTGCATT CGTCGCTGAA 
CAGAAGGTGG AGTATACCCT GGGAGAGGAG TCGGAAGCCC CTGGCCAGAG GGCACTCCCC 
TCCGAGGAGC TGAACAGGCA GCTGGAGAAG CTGCTGAAGG AGGGCAGCAG TAACCAGCGG 
GTGTTCGACT GGATAGAGGC CAACCTGAGT GAGCAGCAGA TAGTATCCAA CACGTTAGTT 
CGAGCCCTCA TGACGGCTGT CTGCTATTCT GCAATTATTT TTGAGACTCC CCTCCGAGTG 
GACGTTGCAG TGCTGAAAGC GCGAGCGAAG CTGCTGCAGA AATACCTGTG TGACGAGCAG 
AAGGAGCTAC AGGCGCTCTA CGCCCTCCAG GCCCTTGTAG TGACCTTAGA ACAGCCTCCC 
AACCTGCTGC GGATGTTCTT TGACGCACTG TATGACGAGG ACGTGGTGAA GGAGGATGCC 
TTCTACAGTT GGGAGAGTAG CAAGGACCCC GCTGAGCAGC AGGGCAAGGG TGTGGCCCTT 
AAATCTGTCA CAGCCTTCTT CAAGTGGCTC CGTGAAGCAG AGGAGGAGTC TGACCACAAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EIF4G1 ( NM_198242.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_937885.1 Homo sapiens eukaryotic translation initiation factor 4 gamma 1 (EIF4G1), transcript variant 4, mRNA.

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CloneID	OHu13773
Clone ID Related Accession (Same CDS sequence)	NM_198242.2 , NM_198242.3
Accession Version	NM_198242.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4308bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-03-03
Organism	Homo sapiens(human)
Product	eukaryotic translation initiation factor 4 gamma 1 isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CR980540.1, AL832754.1, BM475154.1, CX165113.1 and BQ011010.1. On Aug 13, 2010 this sequence version replaced NM_198242.1. Transcript Variant: This variant (4) differs in the 5' UTR, lacks a portion of the 5' coding region, uses a downstream start codon, and uses an alternate in-frame splice site in the central coding region, compared to variant 1. The resulting isoform (3) is shorter at the N-terminus and lacks an internal residue, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.86057.1, AL832754.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAACCAGC CACCCCAGAT TGCTCCCAAG AGGGAGCGTA AGACGATCCG AATTCGAGAT 
CCAAACCAAG GAGGAAAGGA TATCACAGAG GAGATCATGT CTGGGGCCCG CACTGCCTCC 
ACACCCACCC CTCCCCAGAC GGGAGGCGGT CTGGAGCCTC AAGCTAATGG GGAGACGCCC 
CAGGTTGCTG TCATTGTCCG GCCAGATGAC CGGTCACAGG GAGCAATCAT TGCTGACCGG 
CCAGGGCTGC CTGGCCCAGA GCATAGCCCT TCAGAATCCC AGCCTTCGTC GCCTTCTCCG 
ACCCCATCAC CATCCCCAGT CTTGGAACCG GGGTCTGAGC CTAATCTCGC AGTCCTCTCT 
ATTCCTGGGG ACACTATGAC AACTATACAA ATGTCTGTAG AAGAATCAAC CCCCATCTCC 
CGTGAAACTG GGGAGCCATA TCGCCTCTCT CCAGAACCCA CTCCTCTCGC CGAACCCATA 
CTGGAAGTAG AAGTGACACT TAGCAAACCG GTTCCAGAAT CTGAGTTTTC TTCCAGTCCT 
CTCCAGGCTC CCACCCCTTT GGCATCTCAC ACAGTGGAAA TTCATGAGCC TAATGGCATG 
GTCCCATCTG AAGATCTGGA ACCAGAGGTG GAGTCAAGCC CAGAGCTTGC TCCTCCCCCA 
GCTTGCCCCT CCGAATCCCC TGTGCCCATT GCTCCAACTG CCCAACCTGA GGAACTGCTC 
AACGGAGCCC CCTCGCCACC AGCTGTGGAC TTAAGCCCAG TCAGTGAGCC AGAGGAGCAG 
GCCAAGGAGG TGACAGCATC AATGGCGCCC CCCACCATCC CCTCTGCTAC TCCAGCTACG 
GCTCCTTCAG CTACTTCCCC AGCTCAGGAG GAGGAAATGG AAGAAGAAGA AGAAGAGGAA 
GAAGGAGAAG CAGGAGAAGC AGGAGAAGCT GAGAGTGAGA AAGGAGGAGA GGAACTGCTC 
CCCCCAGAGA GTACCCCTAT TCCAGCCAAC TTGTCTCAGA ATTTGGAGGC AGCAGCAGCC 
ACTCAAGTGG CAGTATCTGT GCCAAAGAGG AGACGGAAAA TTAAGGAGCT AAATAAGAAG 
GAGGCTGTTG GAGACCTTCT GGATGCCTTC AAGGAGGCGA ACCCGGCAGT ACCAGAGGTG 
GAAAATCAGC CTCCTGCAGG CAGCAATCCA GGCCCAGAGT CTGAGGGCAG TGGTGTGCCC 
CCACGTCCTG AGGAAGCAGA TGAGACCTGG GACTCAAAGG AAGACAAAAT TCACAATGCT 
GAGAACATCC AGCCCGGGGA ACAGAAGTAT GAATATAAGT CAGATCAGTG GAAGCCTCTA 
AACCTAGAGG AGAAAAAACG TTACGACCGT GAGTTCCTGC TTGGTTTTCA GTTCATCTTT 
GCCAGTATGC AGAAGCCAGA GGGATTGCCA CATATCAGTG ACGTGGTGCT GGACAAGGCC 
AATAAAACAC CACTGCGGCC ACTGGATCCC ACTAGACTAC AAGGCATAAA TTGTGGCCCA 
GACTTCACTC CATCCTTTGC CAACCTTGGC CGGACAACCC TTAGCACCCG TGGGCCCCCA 
AGGGGTGGGC CAGGTGGGGA GCTGCCCCGT GGGCCGGCTG GCCTGGGACC CCGGCGCTCT 
CAGCAGGGAC CCCGAAAAGA ACCACGCAAG ATCATTGCCA CAGTGTTAAT GACCGAAGAT 
ATAAAACTGA ACAAAGCAGA GAAAGCCTGG AAACCCAGCA GCAAGCGGAC GGCGGCTGAT 
AAGGATCGAG GGGAAGAAGA TGCTGATGGC AGCAAAACCC AGGACCTATT CCGCAGGGTG 
CGCTCCATCC TGAATAAACT GACACCCCAG ATGTTCCAGC AGCTGATGAA GCAAGTGACG 
CAGCTGGCCA TCGACACCGA GGAACGCCTC AAAGGGGTCA TTGACCTCAT TTTTGAGAAG 
GCCATTTCAG AGCCCAACTT CTCTGTGGCC TATGCCAACA TGTGCCGCTG CCTCATGGCG 
CTGAAAGTGC CCACTACGGA AAAGCCAACA GTGACTGTGA ACTTCCGAAA GCTGTTGTTG 
AATCGATGTC AGAAGGAGTT TGAGAAAGAC AAAGATGATG ATGAGGTTTT TGAGAAGAAG 
CAAAAAGAGA TGGATGAAGC TGCTACGGCA GAGGAACGAG GACGCCTGAA GGAAGAGCTG 
GAAGAGGCTC GGGACATAGC CCGGCGGCGC TCTTTAGGGA ATATCAAGTT TATTGGAGAG 
TTGTTCAAAC TGAAGATGTT AACAGAGGCA ATAATGCATG ACTGTGTGGT CAAACTGCTT 
AAGAACCATG ATGAAGAGTC CCTTGAGTGC CTTTGTCGTC TGCTCACCAC CATTGGCAAA 
GACCTGGACT TTGAAAAAGC CAAGCCCCGA ATGGATCAGT ATTTCAACCA GATGGAAAAA 
ATCATTAAAG AAAAGAAGAC GTCATCCCGC ATCCGCTTTA TGCTGCAGGA CGTGCTGGAT 
CTGCGAGGGA GCAATTGGGT GCCACGCCGA GGGGATCAGG GTCCCAAGAC CATTGACCAG 
ATCCATAAGG AGGCTGAGAT GGAAGAACAT CGAGAGCACA TCAAAGTGCA GCAGCTCATG 
GCCAAGGGCA GTGACAAGCG TCGGGGCGGT CCTCCAGGCC CTCCCATCAG CCGTGGACTT 
CCCCTTGTGG ATGATGGTGG CTGGAACACA GTTCCCATCA GCAAAGGTAG CCGCCCCATT 
GACACCTCAC GACTCACCAA GATCACCAAG CCTGGCTCCA TCGATTCTAA CAACCAGCTC 
TTTGCACCTG GAGGGCGACT GAGCTGGGGC AAGGGCAGCA GCGGAGGCTC AGGAGCCAAG 
CCCTCAGACG CAGCATCAGA AGCTGCTCGC CCAGCTACTA GTACTTTGAA TCGCTTCTCA 
GCCCTTCAAC AAGCGGTACC CACAGAAAGC ACAGATAATA GACGTGTGGT GCAGAGGAGT 
AGCTTGAGCC GAGAACGAGG CGAGAAAGCT GGAGACCGAG GAGACCGCCT AGAGCGGAGT 
GAACGGGGAG GGGACCGTGG GGACCGGCTT GATCGTGCGC GGACACCTGC TACCAAGCGG 
AGCTTCAGCA AGGAAGTGGA GGAGCGGAGT AGAGAACGGC CCTCCCAGCC TGAGGGGCTG 
CGCAAGGCAG CTAGCCTCAC GGAGGATCGG GACCGTGGGC GGGATGCCGT GAAGCGAGAA 
GCTGCCCTAC CCCCAGTGAG CCCCCTGAAG GCGGCTCTCT CTGAGGAGGA GTTAGAGAAG 
AAATCCAAGG CTATCATTGA GGAATATCTC CATCTCAATG ACATGAAAGA GGCAGTCCAG 
TGCGTGCAGG AGCTGGCCTC ACCCTCCTTG CTCTTCATCT TTGTACGGCA TGGTGTCGAG 
TCTACGCTGG AGCGCAGTGC CATTGCTCGT GAGCATATGG GGCAGCTGCT GCACCAGCTG 
CTCTGTGCTG GGCATCTGTC TACTGCTCAG TACTACCAAG GGTTGTATGA AATCTTGGAA 
TTGGCTGAGG ACATGGAAAT TGACATCCCC CACGTGTGGC TCTACCTAGC GGAACTGGTA 
ACACCCATTC TGCAGGAAGG TGGGGTGCCC ATGGGGGAGC TGTTCAGGGA GATTACAAAG 
CCTCTGAGAC CGTTGGGCAA AGCTGCTTCC CTGTTGCTGG AGATCCTGGG CCTCCTGTGC 
AAAAGCATGG GTCCTAAAAA GGTGGGGACG CTGTGGCGAG AAGCCGGGCT TAGCTGGAAG 
GAATTTCTAC CTGAAGGCCA GGACATTGGT GCATTCGTCG CTGAACAGAA GGTGGAGTAT 
ACCCTGGGAG AGGAGTCGGA AGCCCCTGGC CAGAGGGCAC TCCCCTCCGA GGAGCTGAAC 
AGGCAGCTGG AGAAGCTGCT GAAGGAGGGC AGCAGTAACC AGCGGGTGTT CGACTGGATA 
GAGGCCAACC TGAGTGAGCA GCAGATAGTA TCCAACACGT TAGTTCGAGC CCTCATGACG 
GCTGTCTGCT ATTCTGCAAT TATTTTTGAG ACTCCCCTCC GAGTGGACGT TGCAGTGCTG 
AAAGCGCGAG CGAAGCTGCT GCAGAAATAC CTGTGTGACG AGCAGAAGGA GCTACAGGCG 
CTCTACGCCC TCCAGGCCCT TGTAGTGACC TTAGAACAGC CTCCCAACCT GCTGCGGATG 
TTCTTTGACG CACTGTATGA CGAGGACGTG GTGAAGGAGG ATGCCTTCTA CAGTTGGGAG 
AGTAGCAAGG ACCCCGCTGA GCAGCAGGGC AAGGGTGTGG CCCTTAAATC TGTCACAGCC 
TTCTTCAAGT GGCTCCGTGA AGCAGAGGAG GAGTCTGACC ACAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_937885.1
CDS	349..4656
Translation	MNQPPQIAPKRERKTIRIRDPNQGGKDITEEIMSGARTASTPTPPQTGGGLEPQANGETPQVAVIVRPDDRSQGAIIADRPGLPGPEHSPSESQPSSPSPTPSPSPVLEPGSEPNLAVLSIPGDTMTTIQMSVEESTPISRETGEPYRLSPEPTPLAEPILEVEVTLSKPVPESEFSSSPLQAPTPLASHTVEIHEPNGMVPSEDLEPEVESSPELAPPPACPSESPVPIAPTAQPEELLNGAPSPPAVDLSPVSEPEEQAKEVTASMAPPTIPSATPATAPSATSPAQEEEMEEEEEEEEGEAGEAGEAESEKGGEELLPPESTPIPANLSQNLEAAAATQVAVSVPKRRRKIKELNKKEAVGDLLDAFKEANPAVPEVENQPPAGSNPGPESEGSGVPPRPEEADETWDSKEDKIHNAENIQPGEQKYEYKSDQWKPLNLEEKKRYDREFLLGFQFIFASMQKPEGLPHISDVVLDKANKTPLRPLDPTRLQGINCGPDFTPSFANLGRTTLSTRGPPRGGPGGELPRGPAGLGPRRSQQGPRKEPRKIIATVLMTEDIKLNKAEKAWKPSSKRTAADKDRGEEDADGSKTQDLFRRVRSILNKLTPQMFQQLMKQVTQLAIDTEERLKGVIDLIFEKAISEPNFSVAYANMCRCLMALKVPTTEKPTVTVNFRKLLLNRCQKEFEKDKDDDEVFEKKQKEMDEAATAEERGRLKEELEEARDIARRRSLGNIKFIGELFKLKMLTEAIMHDCVVKLLKNHDEESLECLCRLLTTIGKDLDFEKAKPRMDQYFNQMEKIIKEKKTSSRIRFMLQDVLDLRGSNWVPRRGDQGPKTIDQIHKEAEMEEHREHIKVQQLMAKGSDKRRGGPPGPPISRGLPLVDDGGWNTVPISKGSRPIDTSRLTKITKPGSIDSNNQLFAPGGRLSWGKGSSGGSGAKPSDAASEAARPATSTLNRFSALQQAVPTESTDNRRVVQRSSLSRERGEKAGDRGDRLERSERGGDRGDRLDRARTPATKRSFSKEVEERSRERPSQPEGLRKAASLTEDRDRGRDAVKREAALPPVSPLKAALSEEELEKKSKAIIEEYLHLNDMKEAVQCVQELASPSLLFIFVRHGVESTLERSAIAREHMGQLLHQLLCAGHLSTAQYYQGLYEILELAEDMEIDIPHVWLYLAELVTPILQEGGVPMGELFREITKPLRPLGKAASLLLEILGLLCKSMGPKKVGTLWREAGLSWKEFLPEGQDIGAFVAEQKVEYTLGEESEAPGQRALPSEELNRQLEKLLKEGSSNQRVFDWIEANLSEQQIVSNTLVRALMTAVCYSAIIFETPLRVDVAVLKARAKLLQKYLCDEQKELQALYALQALVVTLEQPPNLLRMFFDALYDEDVVKEDAFYSWESSKDPAEQQGKGVALKSVTAFFKWLREAEEESDHN

Target ORF information:

RefSeq Version	NM_198242.2
Organism	Homo sapiens(human)
Definition	Homo sapiens eukaryotic translation initiation factor 4 gamma 1 (EIF4G1), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_198242.2

ORF Insert Sequence:

ATGAACCAGC CACCCCAGAT TGCTCCCAAG AGGGAGCGTA AGACGATCCG AATTCGAGAT 
CCAAACCAAG GAGGAAAGGA TATCACAGAG GAGATCATGT CTGGGGCCCG CACTGCCTCC 
ACACCCACCC CTCCCCAGAC GGGAGGCGGT CTGGAGCCTC AAGCTAATGG GGAGACGCCC 
CAGGTTGCTG TCATTGTCCG GCCAGATGAC CGGTCACAGG GAGCAATCAT TGCTGACCGG 
CCAGGGCTGC CTGGCCCAGA GCATAGCCCT TCAGAATCCC AGCCTTCGTC GCCTTCTCCG 
ACCCCATCAC CATCCCCAGT CTTGGAACCG GGGTCTGAGC CTAATCTCGC AGTCCTCTCT 
ATTCCTGGGG ACACTATGAC AACTATACAA ATGTCTGTAG AAGAATCAAC CCCCATCTCC 
CGTGAAACTG GGGAGCCATA TCGCCTCTCT CCAGAACCCA CTCCTCTCGC CGAACCCATA 
CTGGAAGTAG AAGTGACACT TAGCAAACCG GTTCCAGAAT CTGAGTTTTC TTCCAGTCCT 
CTCCAGGCTC CCACCCCTTT GGCATCTCAC ACAGTGGAAA TTCATGAGCC TAATGGCATG 
GTCCCATCTG AAGATCTGGA ACCAGAGGTG GAGTCAAGCC CAGAGCTTGC TCCTCCCCCA 
GCTTGCCCCT CCGAATCCCC TGTGCCCATT GCTCCAACTG CCCAACCTGA GGAACTGCTC 
AACGGAGCCC CCTCGCCACC AGCTGTGGAC TTAAGCCCAG TCAGTGAGCC AGAGGAGCAG 
GCCAAGGAGG TGACAGCATC AATGGCGCCC CCCACCATCC CCTCTGCTAC TCCAGCTACG 
GCTCCTTCAG CTACTTCCCC AGCTCAGGAG GAGGAAATGG AAGAAGAAGA AGAAGAGGAA 
GAAGGAGAAG CAGGAGAAGC AGGAGAAGCT GAGAGTGAGA AAGGAGGAGA GGAACTGCTC 
CCCCCAGAGA GTACCCCTAT TCCAGCCAAC TTGTCTCAGA ATTTGGAGGC AGCAGCAGCC 
ACTCAAGTGG CAGTATCTGT GCCAAAGAGG AGACGGAAAA TTAAGGAGCT AAATAAGAAG 
GAGGCTGTTG GAGACCTTCT GGATGCCTTC AAGGAGGCGA ACCCGGCAGT ACCAGAGGTG 
GAAAATCAGC CTCCTGCAGG CAGCAATCCA GGCCCAGAGT CTGAGGGCAG TGGTGTGCCC 
CCACGTCCTG AGGAAGCAGA TGAGACCTGG GACTCAAAGG AAGACAAAAT TCACAATGCT 
GAGAACATCC AGCCCGGGGA ACAGAAGTAT GAATATAAGT CAGATCAGTG GAAGCCTCTA 
AACCTAGAGG AGAAAAAACG TTACGACCGT GAGTTCCTGC TTGGTTTTCA GTTCATCTTT 
GCCAGTATGC AGAAGCCAGA GGGATTGCCA CATATCAGTG ACGTGGTGCT GGACAAGGCC 
AATAAAACAC CACTGCGGCC ACTGGATCCC ACTAGACTAC AAGGCATAAA TTGTGGCCCA 
GACTTCACTC CATCCTTTGC CAACCTTGGC CGGACAACCC TTAGCACCCG TGGGCCCCCA 
AGGGGTGGGC CAGGTGGGGA GCTGCCCCGT GGGCCGGCTG GCCTGGGACC CCGGCGCTCT 
CAGCAGGGAC CCCGAAAAGA ACCACGCAAG ATCATTGCCA CAGTGTTAAT GACCGAAGAT 
ATAAAACTGA ACAAAGCAGA GAAAGCCTGG AAACCCAGCA GCAAGCGGAC GGCGGCTGAT 
AAGGATCGAG GGGAAGAAGA TGCTGATGGC AGCAAAACCC AGGACCTATT CCGCAGGGTG 
CGCTCCATCC TGAATAAACT GACACCCCAG ATGTTCCAGC AGCTGATGAA GCAAGTGACG 
CAGCTGGCCA TCGACACCGA GGAACGCCTC AAAGGGGTCA TTGACCTCAT TTTTGAGAAG 
GCCATTTCAG AGCCCAACTT CTCTGTGGCC TATGCCAACA TGTGCCGCTG CCTCATGGCG 
CTGAAAGTGC CCACTACGGA AAAGCCAACA GTGACTGTGA ACTTCCGAAA GCTGTTGTTG 
AATCGATGTC AGAAGGAGTT TGAGAAAGAC AAAGATGATG ATGAGGTTTT TGAGAAGAAG 
CAAAAAGAGA TGGATGAAGC TGCTACGGCA GAGGAACGAG GACGCCTGAA GGAAGAGCTG 
GAAGAGGCTC GGGACATAGC CCGGCGGCGC TCTTTAGGGA ATATCAAGTT TATTGGAGAG 
TTGTTCAAAC TGAAGATGTT AACAGAGGCA ATAATGCATG ACTGTGTGGT CAAACTGCTT 
AAGAACCATG ATGAAGAGTC CCTTGAGTGC CTTTGTCGTC TGCTCACCAC CATTGGCAAA 
GACCTGGACT TTGAAAAAGC CAAGCCCCGA ATGGATCAGT ATTTCAACCA GATGGAAAAA 
ATCATTAAAG AAAAGAAGAC GTCATCCCGC ATCCGCTTTA TGCTGCAGGA CGTGCTGGAT 
CTGCGAGGGA GCAATTGGGT GCCACGCCGA GGGGATCAGG GTCCCAAGAC CATTGACCAG 
ATCCATAAGG AGGCTGAGAT GGAAGAACAT CGAGAGCACA TCAAAGTGCA GCAGCTCATG 
GCCAAGGGCA GTGACAAGCG TCGGGGCGGT CCTCCAGGCC CTCCCATCAG CCGTGGACTT 
CCCCTTGTGG ATGATGGTGG CTGGAACACA GTTCCCATCA GCAAAGGTAG CCGCCCCATT 
GACACCTCAC GACTCACCAA GATCACCAAG CCTGGCTCCA TCGATTCTAA CAACCAGCTC 
TTTGCACCTG GAGGGCGACT GAGCTGGGGC AAGGGCAGCA GCGGAGGCTC AGGAGCCAAG 
CCCTCAGACG CAGCATCAGA AGCTGCTCGC CCAGCTACTA GTACTTTGAA TCGCTTCTCA 
GCCCTTCAAC AAGCGGTACC CACAGAAAGC ACAGATAATA GACGTGTGGT GCAGAGGAGT 
AGCTTGAGCC GAGAACGAGG CGAGAAAGCT GGAGACCGAG GAGACCGCCT AGAGCGGAGT 
GAACGGGGAG GGGACCGTGG GGACCGGCTT GATCGTGCGC GGACACCTGC TACCAAGCGG 
AGCTTCAGCA AGGAAGTGGA GGAGCGGAGT AGAGAACGGC CCTCCCAGCC TGAGGGGCTG 
CGCAAGGCAG CTAGCCTCAC GGAGGATCGG GACCGTGGGC GGGATGCCGT GAAGCGAGAA 
GCTGCCCTAC CCCCAGTGAG CCCCCTGAAG GCGGCTCTCT CTGAGGAGGA GTTAGAGAAG 
AAATCCAAGG CTATCATTGA GGAATATCTC CATCTCAATG ACATGAAAGA GGCAGTCCAG 
TGCGTGCAGG AGCTGGCCTC ACCCTCCTTG CTCTTCATCT TTGTACGGCA TGGTGTCGAG 
TCTACGCTGG AGCGCAGTGC CATTGCTCGT GAGCATATGG GGCAGCTGCT GCACCAGCTG 
CTCTGTGCTG GGCATCTGTC TACTGCTCAG TACTACCAAG GGTTGTATGA AATCTTGGAA 
TTGGCTGAGG ACATGGAAAT TGACATCCCC CACGTGTGGC TCTACCTAGC GGAACTGGTA 
ACACCCATTC TGCAGGAAGG TGGGGTGCCC ATGGGGGAGC TGTTCAGGGA GATTACAAAG 
CCTCTGAGAC CGTTGGGCAA AGCTGCTTCC CTGTTGCTGG AGATCCTGGG CCTCCTGTGC 
AAAAGCATGG GTCCTAAAAA GGTGGGGACG CTGTGGCGAG AAGCCGGGCT TAGCTGGAAG 
GAATTTCTAC CTGAAGGCCA GGACATTGGT GCATTCGTCG CTGAACAGAA GGTGGAGTAT 
ACCCTGGGAG AGGAGTCGGA AGCCCCTGGC CAGAGGGCAC TCCCCTCCGA GGAGCTGAAC 
AGGCAGCTGG AGAAGCTGCT GAAGGAGGGC AGCAGTAACC AGCGGGTGTT CGACTGGATA 
GAGGCCAACC TGAGTGAGCA GCAGATAGTA TCCAACACGT TAGTTCGAGC CCTCATGACG 
GCTGTCTGCT ATTCTGCAAT TATTTTTGAG ACTCCCCTCC GAGTGGACGT TGCAGTGCTG 
AAAGCGCGAG CGAAGCTGCT GCAGAAATAC CTGTGTGACG AGCAGAAGGA GCTACAGGCG 
CTCTACGCCC TCCAGGCCCT TGTAGTGACC TTAGAACAGC CTCCCAACCT GCTGCGGATG 
TTCTTTGACG CACTGTATGA CGAGGACGTG GTGAAGGAGG ATGCCTTCTA CAGTTGGGAG 
AGTAGCAAGG ACCCCGCTGA GCAGCAGGGC AAGGGTGTGG CCCTTAAATC TGTCACAGCC 
TTCTTCAAGT GGCTCCGTGA AGCAGAGGAG GAGTCTGACC ACAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EIF4G1 ( NM_198242.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_937885.1 Homo sapiens eukaryotic translation initiation factor 4 gamma 1 (EIF4G1), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OHu13773
Clone ID Related Accession (Same CDS sequence)	NM_198242.2 , NM_198242.3
Accession Version	NM_198242.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4308bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-29
Organism	Homo sapiens(human)
Product	eukaryotic translation initiation factor 4 gamma 1 isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC078797.20. On Jun 2, 2019 this sequence version replaced NM_198242.2. Transcript Variant: This variant (4) differs in the 5' UTR, lacks a portion of the 5' coding region, uses a downstream start codon, and uses an alternate in-frame splice site in the central coding region, compared to variant 1. The resulting isoform (3) is shorter at the N-terminus and lacks an internal residue, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AL832754.1, SRR1803617.86057.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAACCAGC CACCCCAGAT TGCTCCCAAG AGGGAGCGTA AGACGATCCG AATTCGAGAT 
CCAAACCAAG GAGGAAAGGA TATCACAGAG GAGATCATGT CTGGGGCCCG CACTGCCTCC 
ACACCCACCC CTCCCCAGAC GGGAGGCGGT CTGGAGCCTC AAGCTAATGG GGAGACGCCC 
CAGGTTGCTG TCATTGTCCG GCCAGATGAC CGGTCACAGG GAGCAATCAT TGCTGACCGG 
CCAGGGCTGC CTGGCCCAGA GCATAGCCCT TCAGAATCCC AGCCTTCGTC GCCTTCTCCG 
ACCCCATCAC CATCCCCAGT CTTGGAACCG GGGTCTGAGC CTAATCTCGC AGTCCTCTCT 
ATTCCTGGGG ACACTATGAC AACTATACAA ATGTCTGTAG AAGAATCAAC CCCCATCTCC 
CGTGAAACTG GGGAGCCATA TCGCCTCTCT CCAGAACCCA CTCCTCTCGC CGAACCCATA 
CTGGAAGTAG AAGTGACACT TAGCAAACCG GTTCCAGAAT CTGAGTTTTC TTCCAGTCCT 
CTCCAGGCTC CCACCCCTTT GGCATCTCAC ACAGTGGAAA TTCATGAGCC TAATGGCATG 
GTCCCATCTG AAGATCTGGA ACCAGAGGTG GAGTCAAGCC CAGAGCTTGC TCCTCCCCCA 
GCTTGCCCCT CCGAATCCCC TGTGCCCATT GCTCCAACTG CCCAACCTGA GGAACTGCTC 
AACGGAGCCC CCTCGCCACC AGCTGTGGAC TTAAGCCCAG TCAGTGAGCC AGAGGAGCAG 
GCCAAGGAGG TGACAGCATC AATGGCGCCC CCCACCATCC CCTCTGCTAC TCCAGCTACG 
GCTCCTTCAG CTACTTCCCC AGCTCAGGAG GAGGAAATGG AAGAAGAAGA AGAAGAGGAA 
GAAGGAGAAG CAGGAGAAGC AGGAGAAGCT GAGAGTGAGA AAGGAGGAGA GGAACTGCTC 
CCCCCAGAGA GTACCCCTAT TCCAGCCAAC TTGTCTCAGA ATTTGGAGGC AGCAGCAGCC 
ACTCAAGTGG CAGTATCTGT GCCAAAGAGG AGACGGAAAA TTAAGGAGCT AAATAAGAAG 
GAGGCTGTTG GAGACCTTCT GGATGCCTTC AAGGAGGCGA ACCCGGCAGT ACCAGAGGTG 
GAAAATCAGC CTCCTGCAGG CAGCAATCCA GGCCCAGAGT CTGAGGGCAG TGGTGTGCCC 
CCACGTCCTG AGGAAGCAGA TGAGACCTGG GACTCAAAGG AAGACAAAAT TCACAATGCT 
GAGAACATCC AGCCCGGGGA ACAGAAGTAT GAATATAAGT CAGATCAGTG GAAGCCTCTA 
AACCTAGAGG AGAAAAAACG TTACGACCGT GAGTTCCTGC TTGGTTTTCA GTTCATCTTT 
GCCAGTATGC AGAAGCCAGA GGGATTGCCA CATATCAGTG ACGTGGTGCT GGACAAGGCC 
AATAAAACAC CACTGCGGCC ACTGGATCCC ACTAGACTAC AAGGCATAAA TTGTGGCCCA 
GACTTCACTC CATCCTTTGC CAACCTTGGC CGGACAACCC TTAGCACCCG TGGGCCCCCA 
AGGGGTGGGC CAGGTGGGGA GCTGCCCCGT GGGCCGGCTG GCCTGGGACC CCGGCGCTCT 
CAGCAGGGAC CCCGAAAAGA ACCACGCAAG ATCATTGCCA CAGTGTTAAT GACCGAAGAT 
ATAAAACTGA ACAAAGCAGA GAAAGCCTGG AAACCCAGCA GCAAGCGGAC GGCGGCTGAT 
AAGGATCGAG GGGAAGAAGA TGCTGATGGC AGCAAAACCC AGGACCTATT CCGCAGGGTG 
CGCTCCATCC TGAATAAACT GACACCCCAG ATGTTCCAGC AGCTGATGAA GCAAGTGACG 
CAGCTGGCCA TCGACACCGA GGAACGCCTC AAAGGGGTCA TTGACCTCAT TTTTGAGAAG 
GCCATTTCAG AGCCCAACTT CTCTGTGGCC TATGCCAACA TGTGCCGCTG CCTCATGGCG 
CTGAAAGTGC CCACTACGGA AAAGCCAACA GTGACTGTGA ACTTCCGAAA GCTGTTGTTG 
AATCGATGTC AGAAGGAGTT TGAGAAAGAC AAAGATGATG ATGAGGTTTT TGAGAAGAAG 
CAAAAAGAGA TGGATGAAGC TGCTACGGCA GAGGAACGAG GACGCCTGAA GGAAGAGCTG 
GAAGAGGCTC GGGACATAGC CCGGCGGCGC TCTTTAGGGA ATATCAAGTT TATTGGAGAG 
TTGTTCAAAC TGAAGATGTT AACAGAGGCA ATAATGCATG ACTGTGTGGT CAAACTGCTT 
AAGAACCATG ATGAAGAGTC CCTTGAGTGC CTTTGTCGTC TGCTCACCAC CATTGGCAAA 
GACCTGGACT TTGAAAAAGC CAAGCCCCGA ATGGATCAGT ATTTCAACCA GATGGAAAAA 
ATCATTAAAG AAAAGAAGAC GTCATCCCGC ATCCGCTTTA TGCTGCAGGA CGTGCTGGAT 
CTGCGAGGGA GCAATTGGGT GCCACGCCGA GGGGATCAGG GTCCCAAGAC CATTGACCAG 
ATCCATAAGG AGGCTGAGAT GGAAGAACAT CGAGAGCACA TCAAAGTGCA GCAGCTCATG 
GCCAAGGGCA GTGACAAGCG TCGGGGCGGT CCTCCAGGCC CTCCCATCAG CCGTGGACTT 
CCCCTTGTGG ATGATGGTGG CTGGAACACA GTTCCCATCA GCAAAGGTAG CCGCCCCATT 
GACACCTCAC GACTCACCAA GATCACCAAG CCTGGCTCCA TCGATTCTAA CAACCAGCTC 
TTTGCACCTG GAGGGCGACT GAGCTGGGGC AAGGGCAGCA GCGGAGGCTC AGGAGCCAAG 
CCCTCAGACG CAGCATCAGA AGCTGCTCGC CCAGCTACTA GTACTTTGAA TCGCTTCTCA 
GCCCTTCAAC AAGCGGTACC CACAGAAAGC ACAGATAATA GACGTGTGGT GCAGAGGAGT 
AGCTTGAGCC GAGAACGAGG CGAGAAAGCT GGAGACCGAG GAGACCGCCT AGAGCGGAGT 
GAACGGGGAG GGGACCGTGG GGACCGGCTT GATCGTGCGC GGACACCTGC TACCAAGCGG 
AGCTTCAGCA AGGAAGTGGA GGAGCGGAGT AGAGAACGGC CCTCCCAGCC TGAGGGGCTG 
CGCAAGGCAG CTAGCCTCAC GGAGGATCGG GACCGTGGGC GGGATGCCGT GAAGCGAGAA 
GCTGCCCTAC CCCCAGTGAG CCCCCTGAAG GCGGCTCTCT CTGAGGAGGA GTTAGAGAAG 
AAATCCAAGG CTATCATTGA GGAATATCTC CATCTCAATG ACATGAAAGA GGCAGTCCAG 
TGCGTGCAGG AGCTGGCCTC ACCCTCCTTG CTCTTCATCT TTGTACGGCA TGGTGTCGAG 
TCTACGCTGG AGCGCAGTGC CATTGCTCGT GAGCATATGG GGCAGCTGCT GCACCAGCTG 
CTCTGTGCTG GGCATCTGTC TACTGCTCAG TACTACCAAG GGTTGTATGA AATCTTGGAA 
TTGGCTGAGG ACATGGAAAT TGACATCCCC CACGTGTGGC TCTACCTAGC GGAACTGGTA 
ACACCCATTC TGCAGGAAGG TGGGGTGCCC ATGGGGGAGC TGTTCAGGGA GATTACAAAG 
CCTCTGAGAC CGTTGGGCAA AGCTGCTTCC CTGTTGCTGG AGATCCTGGG CCTCCTGTGC 
AAAAGCATGG GTCCTAAAAA GGTGGGGACG CTGTGGCGAG AAGCCGGGCT TAGCTGGAAG 
GAATTTCTAC CTGAAGGCCA GGACATTGGT GCATTCGTCG CTGAACAGAA GGTGGAGTAT 
ACCCTGGGAG AGGAGTCGGA AGCCCCTGGC CAGAGGGCAC TCCCCTCCGA GGAGCTGAAC 
AGGCAGCTGG AGAAGCTGCT GAAGGAGGGC AGCAGTAACC AGCGGGTGTT CGACTGGATA 
GAGGCCAACC TGAGTGAGCA GCAGATAGTA TCCAACACGT TAGTTCGAGC CCTCATGACG 
GCTGTCTGCT ATTCTGCAAT TATTTTTGAG ACTCCCCTCC GAGTGGACGT TGCAGTGCTG 
AAAGCGCGAG CGAAGCTGCT GCAGAAATAC CTGTGTGACG AGCAGAAGGA GCTACAGGCG 
CTCTACGCCC TCCAGGCCCT TGTAGTGACC TTAGAACAGC CTCCCAACCT GCTGCGGATG 
TTCTTTGACG CACTGTATGA CGAGGACGTG GTGAAGGAGG ATGCCTTCTA CAGTTGGGAG 
AGTAGCAAGG ACCCCGCTGA GCAGCAGGGC AAGGGTGTGG CCCTTAAATC TGTCACAGCC 
TTCTTCAAGT GGCTCCGTGA AGCAGAGGAG GAGTCTGACC ACAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_937885.1
CDS	238..4545
Translation	MNQPPQIAPKRERKTIRIRDPNQGGKDITEEIMSGARTASTPTPPQTGGGLEPQANGETPQVAVIVRPDDRSQGAIIADRPGLPGPEHSPSESQPSSPSPTPSPSPVLEPGSEPNLAVLSIPGDTMTTIQMSVEESTPISRETGEPYRLSPEPTPLAEPILEVEVTLSKPVPESEFSSSPLQAPTPLASHTVEIHEPNGMVPSEDLEPEVESSPELAPPPACPSESPVPIAPTAQPEELLNGAPSPPAVDLSPVSEPEEQAKEVTASMAPPTIPSATPATAPSATSPAQEEEMEEEEEEEEGEAGEAGEAESEKGGEELLPPESTPIPANLSQNLEAAAATQVAVSVPKRRRKIKELNKKEAVGDLLDAFKEANPAVPEVENQPPAGSNPGPESEGSGVPPRPEEADETWDSKEDKIHNAENIQPGEQKYEYKSDQWKPLNLEEKKRYDREFLLGFQFIFASMQKPEGLPHISDVVLDKANKTPLRPLDPTRLQGINCGPDFTPSFANLGRTTLSTRGPPRGGPGGELPRGPAGLGPRRSQQGPRKEPRKIIATVLMTEDIKLNKAEKAWKPSSKRTAADKDRGEEDADGSKTQDLFRRVRSILNKLTPQMFQQLMKQVTQLAIDTEERLKGVIDLIFEKAISEPNFSVAYANMCRCLMALKVPTTEKPTVTVNFRKLLLNRCQKEFEKDKDDDEVFEKKQKEMDEAATAEERGRLKEELEEARDIARRRSLGNIKFIGELFKLKMLTEAIMHDCVVKLLKNHDEESLECLCRLLTTIGKDLDFEKAKPRMDQYFNQMEKIIKEKKTSSRIRFMLQDVLDLRGSNWVPRRGDQGPKTIDQIHKEAEMEEHREHIKVQQLMAKGSDKRRGGPPGPPISRGLPLVDDGGWNTVPISKGSRPIDTSRLTKITKPGSIDSNNQLFAPGGRLSWGKGSSGGSGAKPSDAASEAARPATSTLNRFSALQQAVPTESTDNRRVVQRSSLSRERGEKAGDRGDRLERSERGGDRGDRLDRARTPATKRSFSKEVEERSRERPSQPEGLRKAASLTEDRDRGRDAVKREAALPPVSPLKAALSEEELEKKSKAIIEEYLHLNDMKEAVQCVQELASPSLLFIFVRHGVESTLERSAIAREHMGQLLHQLLCAGHLSTAQYYQGLYEILELAEDMEIDIPHVWLYLAELVTPILQEGGVPMGELFREITKPLRPLGKAASLLLEILGLLCKSMGPKKVGTLWREAGLSWKEFLPEGQDIGAFVAEQKVEYTLGEESEAPGQRALPSEELNRQLEKLLKEGSSNQRVFDWIEANLSEQQIVSNTLVRALMTAVCYSAIIFETPLRVDVAVLKARAKLLQKYLCDEQKELQALYALQALVVTLEQPPNLLRMFFDALYDEDVVKEDAFYSWESSKDPAEQQGKGVALKSVTAFFKWLREAEEESDHN

Target ORF information:

RefSeq Version	NM_198242.3
Organism	Homo sapiens(human)
Definition	Homo sapiens eukaryotic translation initiation factor 4 gamma 1 (EIF4G1), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_198242.3

ORF Insert Sequence:

ATGAACCAGC CACCCCAGAT TGCTCCCAAG AGGGAGCGTA AGACGATCCG AATTCGAGAT 
CCAAACCAAG GAGGAAAGGA TATCACAGAG GAGATCATGT CTGGGGCCCG CACTGCCTCC 
ACACCCACCC CTCCCCAGAC GGGAGGCGGT CTGGAGCCTC AAGCTAATGG GGAGACGCCC 
CAGGTTGCTG TCATTGTCCG GCCAGATGAC CGGTCACAGG GAGCAATCAT TGCTGACCGG 
CCAGGGCTGC CTGGCCCAGA GCATAGCCCT TCAGAATCCC AGCCTTCGTC GCCTTCTCCG 
ACCCCATCAC CATCCCCAGT CTTGGAACCG GGGTCTGAGC CTAATCTCGC AGTCCTCTCT 
ATTCCTGGGG ACACTATGAC AACTATACAA ATGTCTGTAG AAGAATCAAC CCCCATCTCC 
CGTGAAACTG GGGAGCCATA TCGCCTCTCT CCAGAACCCA CTCCTCTCGC CGAACCCATA 
CTGGAAGTAG AAGTGACACT TAGCAAACCG GTTCCAGAAT CTGAGTTTTC TTCCAGTCCT 
CTCCAGGCTC CCACCCCTTT GGCATCTCAC ACAGTGGAAA TTCATGAGCC TAATGGCATG 
GTCCCATCTG AAGATCTGGA ACCAGAGGTG GAGTCAAGCC CAGAGCTTGC TCCTCCCCCA 
GCTTGCCCCT CCGAATCCCC TGTGCCCATT GCTCCAACTG CCCAACCTGA GGAACTGCTC 
AACGGAGCCC CCTCGCCACC AGCTGTGGAC TTAAGCCCAG TCAGTGAGCC AGAGGAGCAG 
GCCAAGGAGG TGACAGCATC AATGGCGCCC CCCACCATCC CCTCTGCTAC TCCAGCTACG 
GCTCCTTCAG CTACTTCCCC AGCTCAGGAG GAGGAAATGG AAGAAGAAGA AGAAGAGGAA 
GAAGGAGAAG CAGGAGAAGC AGGAGAAGCT GAGAGTGAGA AAGGAGGAGA GGAACTGCTC 
CCCCCAGAGA GTACCCCTAT TCCAGCCAAC TTGTCTCAGA ATTTGGAGGC AGCAGCAGCC 
ACTCAAGTGG CAGTATCTGT GCCAAAGAGG AGACGGAAAA TTAAGGAGCT AAATAAGAAG 
GAGGCTGTTG GAGACCTTCT GGATGCCTTC AAGGAGGCGA ACCCGGCAGT ACCAGAGGTG 
GAAAATCAGC CTCCTGCAGG CAGCAATCCA GGCCCAGAGT CTGAGGGCAG TGGTGTGCCC 
CCACGTCCTG AGGAAGCAGA TGAGACCTGG GACTCAAAGG AAGACAAAAT TCACAATGCT 
GAGAACATCC AGCCCGGGGA ACAGAAGTAT GAATATAAGT CAGATCAGTG GAAGCCTCTA 
AACCTAGAGG AGAAAAAACG TTACGACCGT GAGTTCCTGC TTGGTTTTCA GTTCATCTTT 
GCCAGTATGC AGAAGCCAGA GGGATTGCCA CATATCAGTG ACGTGGTGCT GGACAAGGCC 
AATAAAACAC CACTGCGGCC ACTGGATCCC ACTAGACTAC AAGGCATAAA TTGTGGCCCA 
GACTTCACTC CATCCTTTGC CAACCTTGGC CGGACAACCC TTAGCACCCG TGGGCCCCCA 
AGGGGTGGGC CAGGTGGGGA GCTGCCCCGT GGGCCGGCTG GCCTGGGACC CCGGCGCTCT 
CAGCAGGGAC CCCGAAAAGA ACCACGCAAG ATCATTGCCA CAGTGTTAAT GACCGAAGAT 
ATAAAACTGA ACAAAGCAGA GAAAGCCTGG AAACCCAGCA GCAAGCGGAC GGCGGCTGAT 
AAGGATCGAG GGGAAGAAGA TGCTGATGGC AGCAAAACCC AGGACCTATT CCGCAGGGTG 
CGCTCCATCC TGAATAAACT GACACCCCAG ATGTTCCAGC AGCTGATGAA GCAAGTGACG 
CAGCTGGCCA TCGACACCGA GGAACGCCTC AAAGGGGTCA TTGACCTCAT TTTTGAGAAG 
GCCATTTCAG AGCCCAACTT CTCTGTGGCC TATGCCAACA TGTGCCGCTG CCTCATGGCG 
CTGAAAGTGC CCACTACGGA AAAGCCAACA GTGACTGTGA ACTTCCGAAA GCTGTTGTTG 
AATCGATGTC AGAAGGAGTT TGAGAAAGAC AAAGATGATG ATGAGGTTTT TGAGAAGAAG 
CAAAAAGAGA TGGATGAAGC TGCTACGGCA GAGGAACGAG GACGCCTGAA GGAAGAGCTG 
GAAGAGGCTC GGGACATAGC CCGGCGGCGC TCTTTAGGGA ATATCAAGTT TATTGGAGAG 
TTGTTCAAAC TGAAGATGTT AACAGAGGCA ATAATGCATG ACTGTGTGGT CAAACTGCTT 
AAGAACCATG ATGAAGAGTC CCTTGAGTGC CTTTGTCGTC TGCTCACCAC CATTGGCAAA 
GACCTGGACT TTGAAAAAGC CAAGCCCCGA ATGGATCAGT ATTTCAACCA GATGGAAAAA 
ATCATTAAAG AAAAGAAGAC GTCATCCCGC ATCCGCTTTA TGCTGCAGGA CGTGCTGGAT 
CTGCGAGGGA GCAATTGGGT GCCACGCCGA GGGGATCAGG GTCCCAAGAC CATTGACCAG 
ATCCATAAGG AGGCTGAGAT GGAAGAACAT CGAGAGCACA TCAAAGTGCA GCAGCTCATG 
GCCAAGGGCA GTGACAAGCG TCGGGGCGGT CCTCCAGGCC CTCCCATCAG CCGTGGACTT 
CCCCTTGTGG ATGATGGTGG CTGGAACACA GTTCCCATCA GCAAAGGTAG CCGCCCCATT 
GACACCTCAC GACTCACCAA GATCACCAAG CCTGGCTCCA TCGATTCTAA CAACCAGCTC 
TTTGCACCTG GAGGGCGACT GAGCTGGGGC AAGGGCAGCA GCGGAGGCTC AGGAGCCAAG 
CCCTCAGACG CAGCATCAGA AGCTGCTCGC CCAGCTACTA GTACTTTGAA TCGCTTCTCA 
GCCCTTCAAC AAGCGGTACC CACAGAAAGC ACAGATAATA GACGTGTGGT GCAGAGGAGT 
AGCTTGAGCC GAGAACGAGG CGAGAAAGCT GGAGACCGAG GAGACCGCCT AGAGCGGAGT 
GAACGGGGAG GGGACCGTGG GGACCGGCTT GATCGTGCGC GGACACCTGC TACCAAGCGG 
AGCTTCAGCA AGGAAGTGGA GGAGCGGAGT AGAGAACGGC CCTCCCAGCC TGAGGGGCTG 
CGCAAGGCAG CTAGCCTCAC GGAGGATCGG GACCGTGGGC GGGATGCCGT GAAGCGAGAA 
GCTGCCCTAC CCCCAGTGAG CCCCCTGAAG GCGGCTCTCT CTGAGGAGGA GTTAGAGAAG 
AAATCCAAGG CTATCATTGA GGAATATCTC CATCTCAATG ACATGAAAGA GGCAGTCCAG 
TGCGTGCAGG AGCTGGCCTC ACCCTCCTTG CTCTTCATCT TTGTACGGCA TGGTGTCGAG 
TCTACGCTGG AGCGCAGTGC CATTGCTCGT GAGCATATGG GGCAGCTGCT GCACCAGCTG 
CTCTGTGCTG GGCATCTGTC TACTGCTCAG TACTACCAAG GGTTGTATGA AATCTTGGAA 
TTGGCTGAGG ACATGGAAAT TGACATCCCC CACGTGTGGC TCTACCTAGC GGAACTGGTA 
ACACCCATTC TGCAGGAAGG TGGGGTGCCC ATGGGGGAGC TGTTCAGGGA GATTACAAAG 
CCTCTGAGAC CGTTGGGCAA AGCTGCTTCC CTGTTGCTGG AGATCCTGGG CCTCCTGTGC 
AAAAGCATGG GTCCTAAAAA GGTGGGGACG CTGTGGCGAG AAGCCGGGCT TAGCTGGAAG 
GAATTTCTAC CTGAAGGCCA GGACATTGGT GCATTCGTCG CTGAACAGAA GGTGGAGTAT 
ACCCTGGGAG AGGAGTCGGA AGCCCCTGGC CAGAGGGCAC TCCCCTCCGA GGAGCTGAAC 
AGGCAGCTGG AGAAGCTGCT GAAGGAGGGC AGCAGTAACC AGCGGGTGTT CGACTGGATA 
GAGGCCAACC TGAGTGAGCA GCAGATAGTA TCCAACACGT TAGTTCGAGC CCTCATGACG 
GCTGTCTGCT ATTCTGCAAT TATTTTTGAG ACTCCCCTCC GAGTGGACGT TGCAGTGCTG 
AAAGCGCGAG CGAAGCTGCT GCAGAAATAC CTGTGTGACG AGCAGAAGGA GCTACAGGCG 
CTCTACGCCC TCCAGGCCCT TGTAGTGACC TTAGAACAGC CTCCCAACCT GCTGCGGATG 
TTCTTTGACG CACTGTATGA CGAGGACGTG GTGAAGGAGG ATGCCTTCTA CAGTTGGGAG 
AGTAGCAAGG ACCCCGCTGA GCAGCAGGGC AAGGGTGTGG CCCTTAAATC TGTCACAGCC 
TTCTTCAAGT GGCTCCGTGA AGCAGAGGAG GAGTCTGACC ACAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EIF4G1 ( NM_001194946.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001181875.1 Homo sapiens eukaryotic translation initiation factor 4 gamma 1 (EIF4G1), transcript variant 6, mRNA.

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CloneID	OHu13775
Clone ID Related Accession (Same CDS sequence)	NM_001194946.1 , NM_001194947.1
Accession Version	NM_001194946.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4821bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-03-03
Organism	Homo sapiens(human)
Product	eukaryotic translation initiation factor 4 gamma 1 isoform 6
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CR980540.1, BC140892.1, BC140896.1 and BQ011010.1. Transcript Variant: This variant (6) differs in the 5' UTR, includes an additional in-frame exon in the 5' coding region, and uses an alternate in-frame splice site in the central coding region, compared to variant 1. The resulting isoform (6) is longer than isoform 1. Both variants 6 and 7 encode the same isoform. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC140892.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAACAAAG CTCCACAGTC CACAGGCCCC CCACCCGCCC CATCCCCCGG ACTCCCACAG 
CCAGCGTTTC CCCCGGGGCA GACAGCGCCG GTGGTGTTCA GTACGCCACA AGCGACACAA 
ATGAACACGC CTTCTCAGCC CCGCCAGGGA GGATTCAGGT CTCTGCAGCA CTTCTACCCT 
AGCCGGGCCC AGCCCCCGAG CAGTGCAGCC TCCCGAGTGC AGAGTGCAGC CCCTGCCCGC 
CCTGGCCCAG CTGCCCATGT CTACCCTGCT GGATCCCAAG TAATGATGAT CCCTTCCCAG 
ATCTCCTACC CAGCCTCCCA GGGGGCCTAC TACATCCCTG GACAGGGGCG TTCCACATAC 
GTTGTCCCGA CACAGCAGTA CCCTGTGCAG CCAGGAGCCC CAGGCTTCTA TCCAGGTGCA 
AGCCCTACAG AATTTGGGAC CTACGCTGGC GCCTACTATC CAGCCCAAGG GGTGCAGCAG 
TTTCCCACTG GCGTGGCCCC CGCCCCAGTT TTGATGAACC AGCCACCCCA GATTGCTCCC 
AAGAGGGAGC GTAAGACGAT CCGAATTCGA GATCCAAACC AAGGAGGAAA GGATATCACA 
GAGGAGATCA TGTCTGGGGC CCGCACTGCC TCCACACCCA CCCCTCCCCA GACGGGAGGC 
GGTCTGGAGC CTCAAGCTAA TGGGGAGACG CCCCAGGTTG CTGTCATTGT CCGGCCAGAT 
GACCGGTCAC AGGGAGCAAT CATTGCTGAC CGGCCAGGGC TGCCTGGCCC AGAGCATAGC 
CCTTCAGAAT CCCAGCCTTC GTCGCCTTCT CCGACCCCAT CACCATCCCC AGTCTTGGAA 
CCGGGGTCTG AGCCTAATCT CGCAGTCCTC TCTATTCCTG GGGACACTAT GACAACTATA 
CAAATGTCTG TAGAAGAATC AACCCCCATC TCCCGTGAAA CTGGGGAGCC ATATCGCCTC 
TCTCCAGAAC CCACTCCTCT CGCCGAACCC ATACTGGAAG TAGAAGTGAC ACTTAGCAAA 
CCGGTTCCAG AATCTGAGTT TTCTTCCAGT CCTCTCCAGG CTCCCACCCC TTTGGCATCT 
CACACAGTGG AAATTCATGA GCCTAATGGC ATGGTCCCAT CTGAAGATCT GGAACCAGAG 
GTGGAGTCAA GCCCAGAGCT TGCTCCTCCC CCAGCTTGCC CCTCCGAATC CCCTGTGCCC 
ATTGCTCCAA CTGCCCAACC TGAGGAACTG CTCAACGGAG CCCCCTCGCC ACCAGCTGTG 
GACTTAAGCC CAGTCAGTGA GCCAGAGGAG CAGGCCAAGG AGGTGACAGC ATCAATGGCG 
CCCCCCACCA TCCCCTCTGC TACTCCAGCT ACGGCTCCTT CAGCTACTTC CCCAGCTCAG 
GAGGAGGAAA TGGAAGAAGA AGAAGAAGAG GAAGAAGGAG AAGCAGGAGA AGCAGGAGAA 
GCTGAGAGTG AGAAAGGAGG AGAGGAACTG CTCCCCCCAG AGAGTACCCC TATTCCAGCC 
AACTTGTCTC AGAATTTGGA GGCAGCAGCA GCCACTCAAG TGGCAGTATC TGTGCCAAAG 
AGGAGACGGA AAATTAAGGA GCTAAATAAG AAGGAGGCTG TTGGAGACCT TCTGGATGCC 
TTCAAGGAGG CGAACCCGGC AGTACCAGAG GTGGAAAATC AGCCTCCTGC AGGCAGCAAT 
CCAGGCCCAG AGTCTGAGGG CAGTGGTGTG CCCCCACGTC CTGAGGAAGC AGATGAGACC 
TGGGACTCAA AGGAAGACAA AATTCACAAT GCTGAGAACA TCCAGCCCGG GGAACAGAAG 
TATGAATATA AGTCAGATCA GTGGAAGCCT CTAAACCTAG AGGAGAAAAA ACGTTACGAC 
CGTGAGTTCC TGCTTGGTTT TCAGTTCATC TTTGCCAGTA TGCAGAAGCC AGAGGGATTG 
CCACATATCA GTGACGTGGT GCTGGACAAG GCCAATAAAA CACCACTGCG GCCACTGGAT 
CCCACTAGAC TACAAGGCAT AAATTGTGGC CCAGACTTCA CTCCATCCTT TGCCAACCTT 
GGCCGGACAA CCCTTAGCAC CCGTGGGCCC CCAAGGGGTG GGCCAGGTGG GGAGCTGCCC 
CGTGGGCCGG CTGGCCTGGG ACCCCGGCGC TCTCAGCAGG GACCCCGAAA AGAACCACGC 
AAGATCATTG CCACAGTGTT AATGACCGAA GATATAAAAC TGAACAAAGC AGAGAAAGCC 
TGGAAACCCA GCAGCAAGCG GACGGCGGCT GATAAGGATC GAGGGGAAGA AGATGCTGAT 
GGCAGCAAAA CCCAGGACCT ATTCCGCAGG GTGCGCTCCA TCCTGAATAA ACTGACACCC 
CAGATGTTCC AGCAGCTGAT GAAGCAAGTG ACGCAGCTGG CCATCGACAC CGAGGAACGC 
CTCAAAGGGG TCATTGACCT CATTTTTGAG AAGGCCATTT CAGAGCCCAA CTTCTCTGTG 
GCCTATGCCA ACATGTGCCG CTGCCTCATG GCGCTGAAAG TGCCCACTAC GGAAAAGCCA 
ACAGTGACTG TGAACTTCCG AAAGCTGTTG TTGAATCGAT GTCAGAAGGA GTTTGAGAAA 
GACAAAGATG ATGATGAGGT TTTTGAGAAG AAGCAAAAAG AGATGGATGA AGCTGCTACG 
GCAGAGGAAC GAGGACGCCT GAAGGAAGAG CTGGAAGAGG CTCGGGACAT AGCCCGGCGG 
CGCTCTTTAG GGAATATCAA GTTTATTGGA GAGTTGTTCA AACTGAAGAT GTTAACAGAG 
GCAATAATGC ATGACTGTGT GGTCAAACTG CTTAAGAACC ATGATGAAGA GTCCCTTGAG 
TGCCTTTGTC GTCTGCTCAC CACCATTGGC AAAGACCTGG ACTTTGAAAA AGCCAAGCCC 
CGAATGGATC AGTATTTCAA CCAGATGGAA AAAATCATTA AAGAAAAGAA GACGTCATCC 
CGCATCCGCT TTATGCTGCA GGACGTGCTG GATCTGCGAG GGAGCAATTG GGTGCCACGC 
CGAGGGGATC AGGGTCCCAA GACCATTGAC CAGATCCATA AGGAGGCTGA GATGGAAGAA 
CATCGAGAGC ACATCAAAGT GCAGCAGCTC ATGGCCAAGG GCAGTGACAA GCGTCGGGGC 
GGTCCTCCAG GCCCTCCCAT CAGCCGTGGA CTTCCCCTTG TGGATGATGG TGGCTGGAAC 
ACAGTTCCCA TCAGCAAAGG TAGCCGCCCC ATTGACACCT CACGACTCAC CAAGATCACC 
AAGCCTGGCT CCATCGATTC TAACAACCAG CTCTTTGCAC CTGGAGGGCG ACTGAGCTGG 
GGCAAGGGCA GCAGCGGAGG CTCAGGAGCC AAGCCCTCAG ACGCAGCATC AGAAGCTGCT 
CGCCCAGCTA CTAGTACTTT GAATCGCTTC TCAGCCCTTC AACAAGCGGT ACCCACAGAA 
AGCACAGATA ATAGACGTGT GGTGCAGAGG AGTAGCTTGA GCCGAGAACG AGGCGAGAAA 
GCTGGAGACC GAGGAGACCG CCTAGAGCGG AGTGAACGGG GAGGGGACCG TGGGGACCGG 
CTTGATCGTG CGCGGACACC TGCTACCAAG CGGAGCTTCA GCAAGGAAGT GGAGGAGCGG 
AGTAGAGAAC GGCCCTCCCA GCCTGAGGGG CTGCGCAAGG CAGCTAGCCT CACGGAGGAT 
CGGGACCGTG GGCGGGATGC CGTGAAGCGA GAAGCTGCCC TACCCCCAGT GAGCCCCCTG 
AAGGCGGCTC TCTCTGAGGA GGAGTTAGAG AAGAAATCCA AGGCTATCAT TGAGGAATAT 
CTCCATCTCA ATGACATGAA AGAGGCAGTC CAGTGCGTGC AGGAGCTGGC CTCACCCTCC 
TTGCTCTTCA TCTTTGTACG GCATGGTGTC GAGTCTACGC TGGAGCGCAG TGCCATTGCT 
CGTGAGCATA TGGGGCAGCT GCTGCACCAG CTGCTCTGTG CTGGGCATCT GTCTACTGCT 
CAGTACTACC AAGGGTTGTA TGAAATCTTG GAATTGGCTG AGGACATGGA AATTGACATC 
CCCCACGTGT GGCTCTACCT AGCGGAACTG GTAACACCCA TTCTGCAGGA AGGTGGGGTG 
CCCATGGGGG AGCTGTTCAG GGAGATTACA AAGCCTCTGA GACCGTTGGG CAAAGCTGCT 
TCCCTGTTGC TGGAGATCCT GGGCCTCCTG TGCAAAAGCA TGGGTCCTAA AAAGGTGGGG 
ACGCTGTGGC GAGAAGCCGG GCTTAGCTGG AAGGAATTTC TACCTGAAGG CCAGGACATT 
GGTGCATTCG TCGCTGAACA GAAGGTGGAG TATACCCTGG GAGAGGAGTC GGAAGCCCCT 
GGCCAGAGGG CACTCCCCTC CGAGGAGCTG AACAGGCAGC TGGAGAAGCT GCTGAAGGAG 
GGCAGCAGTA ACCAGCGGGT GTTCGACTGG ATAGAGGCCA ACCTGAGTGA GCAGCAGATA 
GTATCCAACA CGTTAGTTCG AGCCCTCATG ACGGCTGTCT GCTATTCTGC AATTATTTTT 
GAGACTCCCC TCCGAGTGGA CGTTGCAGTG CTGAAAGCGC GAGCGAAGCT GCTGCAGAAA 
TACCTGTGTG ACGAGCAGAA GGAGCTACAG GCGCTCTACG CCCTCCAGGC CCTTGTAGTG 
ACCTTAGAAC AGCCTCCCAA CCTGCTGCGG ATGTTCTTTG ACGCACTGTA TGACGAGGAC 
GTGGTGAAGG AGGATGCCTT CTACAGTTGG GAGAGTAGCA AGGACCCCGC TGAGCAGCAG 
GGCAAGGGTG TGGCCCTTAA ATCTGTCACA GCCTTCTTCA AGTGGCTCCG TGAAGCAGAG 
GAGGAGTCTG ACCACAACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001181875.1
CDS	272..5092
Translation	MNKAPQSTGPPPAPSPGLPQPAFPPGQTAPVVFSTPQATQMNTPSQPRQGGFRSLQHFYPSRAQPPSSAASRVQSAAPARPGPAAHVYPAGSQVMMIPSQISYPASQGAYYIPGQGRSTYVVPTQQYPVQPGAPGFYPGASPTEFGTYAGAYYPAQGVQQFPTGVAPAPVLMNQPPQIAPKRERKTIRIRDPNQGGKDITEEIMSGARTASTPTPPQTGGGLEPQANGETPQVAVIVRPDDRSQGAIIADRPGLPGPEHSPSESQPSSPSPTPSPSPVLEPGSEPNLAVLSIPGDTMTTIQMSVEESTPISRETGEPYRLSPEPTPLAEPILEVEVTLSKPVPESEFSSSPLQAPTPLASHTVEIHEPNGMVPSEDLEPEVESSPELAPPPACPSESPVPIAPTAQPEELLNGAPSPPAVDLSPVSEPEEQAKEVTASMAPPTIPSATPATAPSATSPAQEEEMEEEEEEEEGEAGEAGEAESEKGGEELLPPESTPIPANLSQNLEAAAATQVAVSVPKRRRKIKELNKKEAVGDLLDAFKEANPAVPEVENQPPAGSNPGPESEGSGVPPRPEEADETWDSKEDKIHNAENIQPGEQKYEYKSDQWKPLNLEEKKRYDREFLLGFQFIFASMQKPEGLPHISDVVLDKANKTPLRPLDPTRLQGINCGPDFTPSFANLGRTTLSTRGPPRGGPGGELPRGPAGLGPRRSQQGPRKEPRKIIATVLMTEDIKLNKAEKAWKPSSKRTAADKDRGEEDADGSKTQDLFRRVRSILNKLTPQMFQQLMKQVTQLAIDTEERLKGVIDLIFEKAISEPNFSVAYANMCRCLMALKVPTTEKPTVTVNFRKLLLNRCQKEFEKDKDDDEVFEKKQKEMDEAATAEERGRLKEELEEARDIARRRSLGNIKFIGELFKLKMLTEAIMHDCVVKLLKNHDEESLECLCRLLTTIGKDLDFEKAKPRMDQYFNQMEKIIKEKKTSSRIRFMLQDVLDLRGSNWVPRRGDQGPKTIDQIHKEAEMEEHREHIKVQQLMAKGSDKRRGGPPGPPISRGLPLVDDGGWNTVPISKGSRPIDTSRLTKITKPGSIDSNNQLFAPGGRLSWGKGSSGGSGAKPSDAASEAARPATSTLNRFSALQQAVPTESTDNRRVVQRSSLSRERGEKAGDRGDRLERSERGGDRGDRLDRARTPATKRSFSKEVEERSRERPSQPEGLRKAASLTEDRDRGRDAVKREAALPPVSPLKAALSEEELEKKSKAIIEEYLHLNDMKEAVQCVQELASPSLLFIFVRHGVESTLERSAIAREHMGQLLHQLLCAGHLSTAQYYQGLYEILELAEDMEIDIPHVWLYLAELVTPILQEGGVPMGELFREITKPLRPLGKAASLLLEILGLLCKSMGPKKVGTLWREAGLSWKEFLPEGQDIGAFVAEQKVEYTLGEESEAPGQRALPSEELNRQLEKLLKEGSSNQRVFDWIEANLSEQQIVSNTLVRALMTAVCYSAIIFETPLRVDVAVLKARAKLLQKYLCDEQKELQALYALQALVVTLEQPPNLLRMFFDALYDEDVVKEDAFYSWESSKDPAEQQGKGVALKSVTAFFKWLREAEEESDHN

Target ORF information:

RefSeq Version	NM_001194946.1
Organism	Homo sapiens(human)
Definition	Homo sapiens eukaryotic translation initiation factor 4 gamma 1 (EIF4G1), transcript variant 6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001194946.1

ORF Insert Sequence:

ATGAACAAAG CTCCACAGTC CACAGGCCCC CCACCCGCCC CATCCCCCGG ACTCCCACAG 
CCAGCGTTTC CCCCGGGGCA GACAGCGCCG GTGGTGTTCA GTACGCCACA AGCGACACAA 
ATGAACACGC CTTCTCAGCC CCGCCAGGGA GGATTCAGGT CTCTGCAGCA CTTCTACCCT 
AGCCGGGCCC AGCCCCCGAG CAGTGCAGCC TCCCGAGTGC AGAGTGCAGC CCCTGCCCGC 
CCTGGCCCAG CTGCCCATGT CTACCCTGCT GGATCCCAAG TAATGATGAT CCCTTCCCAG 
ATCTCCTACC CAGCCTCCCA GGGGGCCTAC TACATCCCTG GACAGGGGCG TTCCACATAC 
GTTGTCCCGA CACAGCAGTA CCCTGTGCAG CCAGGAGCCC CAGGCTTCTA TCCAGGTGCA 
AGCCCTACAG AATTTGGGAC CTACGCTGGC GCCTACTATC CAGCCCAAGG GGTGCAGCAG 
TTTCCCACTG GCGTGGCCCC CGCCCCAGTT TTGATGAACC AGCCACCCCA GATTGCTCCC 
AAGAGGGAGC GTAAGACGAT CCGAATTCGA GATCCAAACC AAGGAGGAAA GGATATCACA 
GAGGAGATCA TGTCTGGGGC CCGCACTGCC TCCACACCCA CCCCTCCCCA GACGGGAGGC 
GGTCTGGAGC CTCAAGCTAA TGGGGAGACG CCCCAGGTTG CTGTCATTGT CCGGCCAGAT 
GACCGGTCAC AGGGAGCAAT CATTGCTGAC CGGCCAGGGC TGCCTGGCCC AGAGCATAGC 
CCTTCAGAAT CCCAGCCTTC GTCGCCTTCT CCGACCCCAT CACCATCCCC AGTCTTGGAA 
CCGGGGTCTG AGCCTAATCT CGCAGTCCTC TCTATTCCTG GGGACACTAT GACAACTATA 
CAAATGTCTG TAGAAGAATC AACCCCCATC TCCCGTGAAA CTGGGGAGCC ATATCGCCTC 
TCTCCAGAAC CCACTCCTCT CGCCGAACCC ATACTGGAAG TAGAAGTGAC ACTTAGCAAA 
CCGGTTCCAG AATCTGAGTT TTCTTCCAGT CCTCTCCAGG CTCCCACCCC TTTGGCATCT 
CACACAGTGG AAATTCATGA GCCTAATGGC ATGGTCCCAT CTGAAGATCT GGAACCAGAG 
GTGGAGTCAA GCCCAGAGCT TGCTCCTCCC CCAGCTTGCC CCTCCGAATC CCCTGTGCCC 
ATTGCTCCAA CTGCCCAACC TGAGGAACTG CTCAACGGAG CCCCCTCGCC ACCAGCTGTG 
GACTTAAGCC CAGTCAGTGA GCCAGAGGAG CAGGCCAAGG AGGTGACAGC ATCAATGGCG 
CCCCCCACCA TCCCCTCTGC TACTCCAGCT ACGGCTCCTT CAGCTACTTC CCCAGCTCAG 
GAGGAGGAAA TGGAAGAAGA AGAAGAAGAG GAAGAAGGAG AAGCAGGAGA AGCAGGAGAA 
GCTGAGAGTG AGAAAGGAGG AGAGGAACTG CTCCCCCCAG AGAGTACCCC TATTCCAGCC 
AACTTGTCTC AGAATTTGGA GGCAGCAGCA GCCACTCAAG TGGCAGTATC TGTGCCAAAG 
AGGAGACGGA AAATTAAGGA GCTAAATAAG AAGGAGGCTG TTGGAGACCT TCTGGATGCC 
TTCAAGGAGG CGAACCCGGC AGTACCAGAG GTGGAAAATC AGCCTCCTGC AGGCAGCAAT 
CCAGGCCCAG AGTCTGAGGG CAGTGGTGTG CCCCCACGTC CTGAGGAAGC AGATGAGACC 
TGGGACTCAA AGGAAGACAA AATTCACAAT GCTGAGAACA TCCAGCCCGG GGAACAGAAG 
TATGAATATA AGTCAGATCA GTGGAAGCCT CTAAACCTAG AGGAGAAAAA ACGTTACGAC 
CGTGAGTTCC TGCTTGGTTT TCAGTTCATC TTTGCCAGTA TGCAGAAGCC AGAGGGATTG 
CCACATATCA GTGACGTGGT GCTGGACAAG GCCAATAAAA CACCACTGCG GCCACTGGAT 
CCCACTAGAC TACAAGGCAT AAATTGTGGC CCAGACTTCA CTCCATCCTT TGCCAACCTT 
GGCCGGACAA CCCTTAGCAC CCGTGGGCCC CCAAGGGGTG GGCCAGGTGG GGAGCTGCCC 
CGTGGGCCGG CTGGCCTGGG ACCCCGGCGC TCTCAGCAGG GACCCCGAAA AGAACCACGC 
AAGATCATTG CCACAGTGTT AATGACCGAA GATATAAAAC TGAACAAAGC AGAGAAAGCC 
TGGAAACCCA GCAGCAAGCG GACGGCGGCT GATAAGGATC GAGGGGAAGA AGATGCTGAT 
GGCAGCAAAA CCCAGGACCT ATTCCGCAGG GTGCGCTCCA TCCTGAATAA ACTGACACCC 
CAGATGTTCC AGCAGCTGAT GAAGCAAGTG ACGCAGCTGG CCATCGACAC CGAGGAACGC 
CTCAAAGGGG TCATTGACCT CATTTTTGAG AAGGCCATTT CAGAGCCCAA CTTCTCTGTG 
GCCTATGCCA ACATGTGCCG CTGCCTCATG GCGCTGAAAG TGCCCACTAC GGAAAAGCCA 
ACAGTGACTG TGAACTTCCG AAAGCTGTTG TTGAATCGAT GTCAGAAGGA GTTTGAGAAA 
GACAAAGATG ATGATGAGGT TTTTGAGAAG AAGCAAAAAG AGATGGATGA AGCTGCTACG 
GCAGAGGAAC GAGGACGCCT GAAGGAAGAG CTGGAAGAGG CTCGGGACAT AGCCCGGCGG 
CGCTCTTTAG GGAATATCAA GTTTATTGGA GAGTTGTTCA AACTGAAGAT GTTAACAGAG 
GCAATAATGC ATGACTGTGT GGTCAAACTG CTTAAGAACC ATGATGAAGA GTCCCTTGAG 
TGCCTTTGTC GTCTGCTCAC CACCATTGGC AAAGACCTGG ACTTTGAAAA AGCCAAGCCC 
CGAATGGATC AGTATTTCAA CCAGATGGAA AAAATCATTA AAGAAAAGAA GACGTCATCC 
CGCATCCGCT TTATGCTGCA GGACGTGCTG GATCTGCGAG GGAGCAATTG GGTGCCACGC 
CGAGGGGATC AGGGTCCCAA GACCATTGAC CAGATCCATA AGGAGGCTGA GATGGAAGAA 
CATCGAGAGC ACATCAAAGT GCAGCAGCTC ATGGCCAAGG GCAGTGACAA GCGTCGGGGC 
GGTCCTCCAG GCCCTCCCAT CAGCCGTGGA CTTCCCCTTG TGGATGATGG TGGCTGGAAC 
ACAGTTCCCA TCAGCAAAGG TAGCCGCCCC ATTGACACCT CACGACTCAC CAAGATCACC 
AAGCCTGGCT CCATCGATTC TAACAACCAG CTCTTTGCAC CTGGAGGGCG ACTGAGCTGG 
GGCAAGGGCA GCAGCGGAGG CTCAGGAGCC AAGCCCTCAG ACGCAGCATC AGAAGCTGCT 
CGCCCAGCTA CTAGTACTTT GAATCGCTTC TCAGCCCTTC AACAAGCGGT ACCCACAGAA 
AGCACAGATA ATAGACGTGT GGTGCAGAGG AGTAGCTTGA GCCGAGAACG AGGCGAGAAA 
GCTGGAGACC GAGGAGACCG CCTAGAGCGG AGTGAACGGG GAGGGGACCG TGGGGACCGG 
CTTGATCGTG CGCGGACACC TGCTACCAAG CGGAGCTTCA GCAAGGAAGT GGAGGAGCGG 
AGTAGAGAAC GGCCCTCCCA GCCTGAGGGG CTGCGCAAGG CAGCTAGCCT CACGGAGGAT 
CGGGACCGTG GGCGGGATGC CGTGAAGCGA GAAGCTGCCC TACCCCCAGT GAGCCCCCTG 
AAGGCGGCTC TCTCTGAGGA GGAGTTAGAG AAGAAATCCA AGGCTATCAT TGAGGAATAT 
CTCCATCTCA ATGACATGAA AGAGGCAGTC CAGTGCGTGC AGGAGCTGGC CTCACCCTCC 
TTGCTCTTCA TCTTTGTACG GCATGGTGTC GAGTCTACGC TGGAGCGCAG TGCCATTGCT 
CGTGAGCATA TGGGGCAGCT GCTGCACCAG CTGCTCTGTG CTGGGCATCT GTCTACTGCT 
CAGTACTACC AAGGGTTGTA TGAAATCTTG GAATTGGCTG AGGACATGGA AATTGACATC 
CCCCACGTGT GGCTCTACCT AGCGGAACTG GTAACACCCA TTCTGCAGGA AGGTGGGGTG 
CCCATGGGGG AGCTGTTCAG GGAGATTACA AAGCCTCTGA GACCGTTGGG CAAAGCTGCT 
TCCCTGTTGC TGGAGATCCT GGGCCTCCTG TGCAAAAGCA TGGGTCCTAA AAAGGTGGGG 
ACGCTGTGGC GAGAAGCCGG GCTTAGCTGG AAGGAATTTC TACCTGAAGG CCAGGACATT 
GGTGCATTCG TCGCTGAACA GAAGGTGGAG TATACCCTGG GAGAGGAGTC GGAAGCCCCT 
GGCCAGAGGG CACTCCCCTC CGAGGAGCTG AACAGGCAGC TGGAGAAGCT GCTGAAGGAG 
GGCAGCAGTA ACCAGCGGGT GTTCGACTGG ATAGAGGCCA ACCTGAGTGA GCAGCAGATA 
GTATCCAACA CGTTAGTTCG AGCCCTCATG ACGGCTGTCT GCTATTCTGC AATTATTTTT 
GAGACTCCCC TCCGAGTGGA CGTTGCAGTG CTGAAAGCGC GAGCGAAGCT GCTGCAGAAA 
TACCTGTGTG ACGAGCAGAA GGAGCTACAG GCGCTCTACG CCCTCCAGGC CCTTGTAGTG 
ACCTTAGAAC AGCCTCCCAA CCTGCTGCGG ATGTTCTTTG ACGCACTGTA TGACGAGGAC 
GTGGTGAAGG AGGATGCCTT CTACAGTTGG GAGAGTAGCA AGGACCCCGC TGAGCAGCAG 
GGCAAGGGTG TGGCCCTTAA ATCTGTCACA GCCTTCTTCA AGTGGCTCCG TGAAGCAGAG 
GAGGAGTCTG ACCACAACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EIF4G1 ( NM_001194947.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001181876.1 Homo sapiens eukaryotic translation initiation factor 4 gamma 1 (EIF4G1), transcript variant 7, mRNA.

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CloneID	OHu13775
Clone ID Related Accession (Same CDS sequence)	NM_001194946.1 , NM_001194947.1
Accession Version	NM_001194947.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4821bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-19
Organism	Homo sapiens(human)
Product	eukaryotic translation initiation factor 4 gamma 1 isoform 6
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CR980540.1, BC140896.1 and BQ011010.1. Transcript Variant: This variant (7) differs in the 5' UTR, includes an additional in-frame exon in the 5' coding region, and uses an alternate in-frame splice site in the central coding region, compared to variant 1. The resulting isoform (6) is longer than isoform 1. Both variants 6 and 7 encode the same isoform. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC140896.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2142348 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAACAAAG CTCCACAGTC CACAGGCCCC CCACCCGCCC CATCCCCCGG ACTCCCACAG 
CCAGCGTTTC CCCCGGGGCA GACAGCGCCG GTGGTGTTCA GTACGCCACA AGCGACACAA 
ATGAACACGC CTTCTCAGCC CCGCCAGGGA GGATTCAGGT CTCTGCAGCA CTTCTACCCT 
AGCCGGGCCC AGCCCCCGAG CAGTGCAGCC TCCCGAGTGC AGAGTGCAGC CCCTGCCCGC 
CCTGGCCCAG CTGCCCATGT CTACCCTGCT GGATCCCAAG TAATGATGAT CCCTTCCCAG 
ATCTCCTACC CAGCCTCCCA GGGGGCCTAC TACATCCCTG GACAGGGGCG TTCCACATAC 
GTTGTCCCGA CACAGCAGTA CCCTGTGCAG CCAGGAGCCC CAGGCTTCTA TCCAGGTGCA 
AGCCCTACAG AATTTGGGAC CTACGCTGGC GCCTACTATC CAGCCCAAGG GGTGCAGCAG 
TTTCCCACTG GCGTGGCCCC CGCCCCAGTT TTGATGAACC AGCCACCCCA GATTGCTCCC 
AAGAGGGAGC GTAAGACGAT CCGAATTCGA GATCCAAACC AAGGAGGAAA GGATATCACA 
GAGGAGATCA TGTCTGGGGC CCGCACTGCC TCCACACCCA CCCCTCCCCA GACGGGAGGC 
GGTCTGGAGC CTCAAGCTAA TGGGGAGACG CCCCAGGTTG CTGTCATTGT CCGGCCAGAT 
GACCGGTCAC AGGGAGCAAT CATTGCTGAC CGGCCAGGGC TGCCTGGCCC AGAGCATAGC 
CCTTCAGAAT CCCAGCCTTC GTCGCCTTCT CCGACCCCAT CACCATCCCC AGTCTTGGAA 
CCGGGGTCTG AGCCTAATCT CGCAGTCCTC TCTATTCCTG GGGACACTAT GACAACTATA 
CAAATGTCTG TAGAAGAATC AACCCCCATC TCCCGTGAAA CTGGGGAGCC ATATCGCCTC 
TCTCCAGAAC CCACTCCTCT CGCCGAACCC ATACTGGAAG TAGAAGTGAC ACTTAGCAAA 
CCGGTTCCAG AATCTGAGTT TTCTTCCAGT CCTCTCCAGG CTCCCACCCC TTTGGCATCT 
CACACAGTGG AAATTCATGA GCCTAATGGC ATGGTCCCAT CTGAAGATCT GGAACCAGAG 
GTGGAGTCAA GCCCAGAGCT TGCTCCTCCC CCAGCTTGCC CCTCCGAATC CCCTGTGCCC 
ATTGCTCCAA CTGCCCAACC TGAGGAACTG CTCAACGGAG CCCCCTCGCC ACCAGCTGTG 
GACTTAAGCC CAGTCAGTGA GCCAGAGGAG CAGGCCAAGG AGGTGACAGC ATCAATGGCG 
CCCCCCACCA TCCCCTCTGC TACTCCAGCT ACGGCTCCTT CAGCTACTTC CCCAGCTCAG 
GAGGAGGAAA TGGAAGAAGA AGAAGAAGAG GAAGAAGGAG AAGCAGGAGA AGCAGGAGAA 
GCTGAGAGTG AGAAAGGAGG AGAGGAACTG CTCCCCCCAG AGAGTACCCC TATTCCAGCC 
AACTTGTCTC AGAATTTGGA GGCAGCAGCA GCCACTCAAG TGGCAGTATC TGTGCCAAAG 
AGGAGACGGA AAATTAAGGA GCTAAATAAG AAGGAGGCTG TTGGAGACCT TCTGGATGCC 
TTCAAGGAGG CGAACCCGGC AGTACCAGAG GTGGAAAATC AGCCTCCTGC AGGCAGCAAT 
CCAGGCCCAG AGTCTGAGGG CAGTGGTGTG CCCCCACGTC CTGAGGAAGC AGATGAGACC 
TGGGACTCAA AGGAAGACAA AATTCACAAT GCTGAGAACA TCCAGCCCGG GGAACAGAAG 
TATGAATATA AGTCAGATCA GTGGAAGCCT CTAAACCTAG AGGAGAAAAA ACGTTACGAC 
CGTGAGTTCC TGCTTGGTTT TCAGTTCATC TTTGCCAGTA TGCAGAAGCC AGAGGGATTG 
CCACATATCA GTGACGTGGT GCTGGACAAG GCCAATAAAA CACCACTGCG GCCACTGGAT 
CCCACTAGAC TACAAGGCAT AAATTGTGGC CCAGACTTCA CTCCATCCTT TGCCAACCTT 
GGCCGGACAA CCCTTAGCAC CCGTGGGCCC CCAAGGGGTG GGCCAGGTGG GGAGCTGCCC 
CGTGGGCCGG CTGGCCTGGG ACCCCGGCGC TCTCAGCAGG GACCCCGAAA AGAACCACGC 
AAGATCATTG CCACAGTGTT AATGACCGAA GATATAAAAC TGAACAAAGC AGAGAAAGCC 
TGGAAACCCA GCAGCAAGCG GACGGCGGCT GATAAGGATC GAGGGGAAGA AGATGCTGAT 
GGCAGCAAAA CCCAGGACCT ATTCCGCAGG GTGCGCTCCA TCCTGAATAA ACTGACACCC 
CAGATGTTCC AGCAGCTGAT GAAGCAAGTG ACGCAGCTGG CCATCGACAC CGAGGAACGC 
CTCAAAGGGG TCATTGACCT CATTTTTGAG AAGGCCATTT CAGAGCCCAA CTTCTCTGTG 
GCCTATGCCA ACATGTGCCG CTGCCTCATG GCGCTGAAAG TGCCCACTAC GGAAAAGCCA 
ACAGTGACTG TGAACTTCCG AAAGCTGTTG TTGAATCGAT GTCAGAAGGA GTTTGAGAAA 
GACAAAGATG ATGATGAGGT TTTTGAGAAG AAGCAAAAAG AGATGGATGA AGCTGCTACG 
GCAGAGGAAC GAGGACGCCT GAAGGAAGAG CTGGAAGAGG CTCGGGACAT AGCCCGGCGG 
CGCTCTTTAG GGAATATCAA GTTTATTGGA GAGTTGTTCA AACTGAAGAT GTTAACAGAG 
GCAATAATGC ATGACTGTGT GGTCAAACTG CTTAAGAACC ATGATGAAGA GTCCCTTGAG 
TGCCTTTGTC GTCTGCTCAC CACCATTGGC AAAGACCTGG ACTTTGAAAA AGCCAAGCCC 
CGAATGGATC AGTATTTCAA CCAGATGGAA AAAATCATTA AAGAAAAGAA GACGTCATCC 
CGCATCCGCT TTATGCTGCA GGACGTGCTG GATCTGCGAG GGAGCAATTG GGTGCCACGC 
CGAGGGGATC AGGGTCCCAA GACCATTGAC CAGATCCATA AGGAGGCTGA GATGGAAGAA 
CATCGAGAGC ACATCAAAGT GCAGCAGCTC ATGGCCAAGG GCAGTGACAA GCGTCGGGGC 
GGTCCTCCAG GCCCTCCCAT CAGCCGTGGA CTTCCCCTTG TGGATGATGG TGGCTGGAAC 
ACAGTTCCCA TCAGCAAAGG TAGCCGCCCC ATTGACACCT CACGACTCAC CAAGATCACC 
AAGCCTGGCT CCATCGATTC TAACAACCAG CTCTTTGCAC CTGGAGGGCG ACTGAGCTGG 
GGCAAGGGCA GCAGCGGAGG CTCAGGAGCC AAGCCCTCAG ACGCAGCATC AGAAGCTGCT 
CGCCCAGCTA CTAGTACTTT GAATCGCTTC TCAGCCCTTC AACAAGCGGT ACCCACAGAA 
AGCACAGATA ATAGACGTGT GGTGCAGAGG AGTAGCTTGA GCCGAGAACG AGGCGAGAAA 
GCTGGAGACC GAGGAGACCG CCTAGAGCGG AGTGAACGGG GAGGGGACCG TGGGGACCGG 
CTTGATCGTG CGCGGACACC TGCTACCAAG CGGAGCTTCA GCAAGGAAGT GGAGGAGCGG 
AGTAGAGAAC GGCCCTCCCA GCCTGAGGGG CTGCGCAAGG CAGCTAGCCT CACGGAGGAT 
CGGGACCGTG GGCGGGATGC CGTGAAGCGA GAAGCTGCCC TACCCCCAGT GAGCCCCCTG 
AAGGCGGCTC TCTCTGAGGA GGAGTTAGAG AAGAAATCCA AGGCTATCAT TGAGGAATAT 
CTCCATCTCA ATGACATGAA AGAGGCAGTC CAGTGCGTGC AGGAGCTGGC CTCACCCTCC 
TTGCTCTTCA TCTTTGTACG GCATGGTGTC GAGTCTACGC TGGAGCGCAG TGCCATTGCT 
CGTGAGCATA TGGGGCAGCT GCTGCACCAG CTGCTCTGTG CTGGGCATCT GTCTACTGCT 
CAGTACTACC AAGGGTTGTA TGAAATCTTG GAATTGGCTG AGGACATGGA AATTGACATC 
CCCCACGTGT GGCTCTACCT AGCGGAACTG GTAACACCCA TTCTGCAGGA AGGTGGGGTG 
CCCATGGGGG AGCTGTTCAG GGAGATTACA AAGCCTCTGA GACCGTTGGG CAAAGCTGCT 
TCCCTGTTGC TGGAGATCCT GGGCCTCCTG TGCAAAAGCA TGGGTCCTAA AAAGGTGGGG 
ACGCTGTGGC GAGAAGCCGG GCTTAGCTGG AAGGAATTTC TACCTGAAGG CCAGGACATT 
GGTGCATTCG TCGCTGAACA GAAGGTGGAG TATACCCTGG GAGAGGAGTC GGAAGCCCCT 
GGCCAGAGGG CACTCCCCTC CGAGGAGCTG AACAGGCAGC TGGAGAAGCT GCTGAAGGAG 
GGCAGCAGTA ACCAGCGGGT GTTCGACTGG ATAGAGGCCA ACCTGAGTGA GCAGCAGATA 
GTATCCAACA CGTTAGTTCG AGCCCTCATG ACGGCTGTCT GCTATTCTGC AATTATTTTT 
GAGACTCCCC TCCGAGTGGA CGTTGCAGTG CTGAAAGCGC GAGCGAAGCT GCTGCAGAAA 
TACCTGTGTG ACGAGCAGAA GGAGCTACAG GCGCTCTACG CCCTCCAGGC CCTTGTAGTG 
ACCTTAGAAC AGCCTCCCAA CCTGCTGCGG ATGTTCTTTG ACGCACTGTA TGACGAGGAC 
GTGGTGAAGG AGGATGCCTT CTACAGTTGG GAGAGTAGCA AGGACCCCGC TGAGCAGCAG 
GGCAAGGGTG TGGCCCTTAA ATCTGTCACA GCCTTCTTCA AGTGGCTCCG TGAAGCAGAG 
GAGGAGTCTG ACCACAACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001181876.1
CDS	215..5035
Translation	MNKAPQSTGPPPAPSPGLPQPAFPPGQTAPVVFSTPQATQMNTPSQPRQGGFRSLQHFYPSRAQPPSSAASRVQSAAPARPGPAAHVYPAGSQVMMIPSQISYPASQGAYYIPGQGRSTYVVPTQQYPVQPGAPGFYPGASPTEFGTYAGAYYPAQGVQQFPTGVAPAPVLMNQPPQIAPKRERKTIRIRDPNQGGKDITEEIMSGARTASTPTPPQTGGGLEPQANGETPQVAVIVRPDDRSQGAIIADRPGLPGPEHSPSESQPSSPSPTPSPSPVLEPGSEPNLAVLSIPGDTMTTIQMSVEESTPISRETGEPYRLSPEPTPLAEPILEVEVTLSKPVPESEFSSSPLQAPTPLASHTVEIHEPNGMVPSEDLEPEVESSPELAPPPACPSESPVPIAPTAQPEELLNGAPSPPAVDLSPVSEPEEQAKEVTASMAPPTIPSATPATAPSATSPAQEEEMEEEEEEEEGEAGEAGEAESEKGGEELLPPESTPIPANLSQNLEAAAATQVAVSVPKRRRKIKELNKKEAVGDLLDAFKEANPAVPEVENQPPAGSNPGPESEGSGVPPRPEEADETWDSKEDKIHNAENIQPGEQKYEYKSDQWKPLNLEEKKRYDREFLLGFQFIFASMQKPEGLPHISDVVLDKANKTPLRPLDPTRLQGINCGPDFTPSFANLGRTTLSTRGPPRGGPGGELPRGPAGLGPRRSQQGPRKEPRKIIATVLMTEDIKLNKAEKAWKPSSKRTAADKDRGEEDADGSKTQDLFRRVRSILNKLTPQMFQQLMKQVTQLAIDTEERLKGVIDLIFEKAISEPNFSVAYANMCRCLMALKVPTTEKPTVTVNFRKLLLNRCQKEFEKDKDDDEVFEKKQKEMDEAATAEERGRLKEELEEARDIARRRSLGNIKFIGELFKLKMLTEAIMHDCVVKLLKNHDEESLECLCRLLTTIGKDLDFEKAKPRMDQYFNQMEKIIKEKKTSSRIRFMLQDVLDLRGSNWVPRRGDQGPKTIDQIHKEAEMEEHREHIKVQQLMAKGSDKRRGGPPGPPISRGLPLVDDGGWNTVPISKGSRPIDTSRLTKITKPGSIDSNNQLFAPGGRLSWGKGSSGGSGAKPSDAASEAARPATSTLNRFSALQQAVPTESTDNRRVVQRSSLSRERGEKAGDRGDRLERSERGGDRGDRLDRARTPATKRSFSKEVEERSRERPSQPEGLRKAASLTEDRDRGRDAVKREAALPPVSPLKAALSEEELEKKSKAIIEEYLHLNDMKEAVQCVQELASPSLLFIFVRHGVESTLERSAIAREHMGQLLHQLLCAGHLSTAQYYQGLYEILELAEDMEIDIPHVWLYLAELVTPILQEGGVPMGELFREITKPLRPLGKAASLLLEILGLLCKSMGPKKVGTLWREAGLSWKEFLPEGQDIGAFVAEQKVEYTLGEESEAPGQRALPSEELNRQLEKLLKEGSSNQRVFDWIEANLSEQQIVSNTLVRALMTAVCYSAIIFETPLRVDVAVLKARAKLLQKYLCDEQKELQALYALQALVVTLEQPPNLLRMFFDALYDEDVVKEDAFYSWESSKDPAEQQGKGVALKSVTAFFKWLREAEEESDHN

Target ORF information:

RefSeq Version	NM_001194947.1
Organism	Homo sapiens(human)
Definition	Homo sapiens eukaryotic translation initiation factor 4 gamma 1 (EIF4G1), transcript variant 7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001194947.1

ORF Insert Sequence:

ATGAACAAAG CTCCACAGTC CACAGGCCCC CCACCCGCCC CATCCCCCGG ACTCCCACAG 
CCAGCGTTTC CCCCGGGGCA GACAGCGCCG GTGGTGTTCA GTACGCCACA AGCGACACAA 
ATGAACACGC CTTCTCAGCC CCGCCAGGGA GGATTCAGGT CTCTGCAGCA CTTCTACCCT 
AGCCGGGCCC AGCCCCCGAG CAGTGCAGCC TCCCGAGTGC AGAGTGCAGC CCCTGCCCGC 
CCTGGCCCAG CTGCCCATGT CTACCCTGCT GGATCCCAAG TAATGATGAT CCCTTCCCAG 
ATCTCCTACC CAGCCTCCCA GGGGGCCTAC TACATCCCTG GACAGGGGCG TTCCACATAC 
GTTGTCCCGA CACAGCAGTA CCCTGTGCAG CCAGGAGCCC CAGGCTTCTA TCCAGGTGCA 
AGCCCTACAG AATTTGGGAC CTACGCTGGC GCCTACTATC CAGCCCAAGG GGTGCAGCAG 
TTTCCCACTG GCGTGGCCCC CGCCCCAGTT TTGATGAACC AGCCACCCCA GATTGCTCCC 
AAGAGGGAGC GTAAGACGAT CCGAATTCGA GATCCAAACC AAGGAGGAAA GGATATCACA 
GAGGAGATCA TGTCTGGGGC CCGCACTGCC TCCACACCCA CCCCTCCCCA GACGGGAGGC 
GGTCTGGAGC CTCAAGCTAA TGGGGAGACG CCCCAGGTTG CTGTCATTGT CCGGCCAGAT 
GACCGGTCAC AGGGAGCAAT CATTGCTGAC CGGCCAGGGC TGCCTGGCCC AGAGCATAGC 
CCTTCAGAAT CCCAGCCTTC GTCGCCTTCT CCGACCCCAT CACCATCCCC AGTCTTGGAA 
CCGGGGTCTG AGCCTAATCT CGCAGTCCTC TCTATTCCTG GGGACACTAT GACAACTATA 
CAAATGTCTG TAGAAGAATC AACCCCCATC TCCCGTGAAA CTGGGGAGCC ATATCGCCTC 
TCTCCAGAAC CCACTCCTCT CGCCGAACCC ATACTGGAAG TAGAAGTGAC ACTTAGCAAA 
CCGGTTCCAG AATCTGAGTT TTCTTCCAGT CCTCTCCAGG CTCCCACCCC TTTGGCATCT 
CACACAGTGG AAATTCATGA GCCTAATGGC ATGGTCCCAT CTGAAGATCT GGAACCAGAG 
GTGGAGTCAA GCCCAGAGCT TGCTCCTCCC CCAGCTTGCC CCTCCGAATC CCCTGTGCCC 
ATTGCTCCAA CTGCCCAACC TGAGGAACTG CTCAACGGAG CCCCCTCGCC ACCAGCTGTG 
GACTTAAGCC CAGTCAGTGA GCCAGAGGAG CAGGCCAAGG AGGTGACAGC ATCAATGGCG 
CCCCCCACCA TCCCCTCTGC TACTCCAGCT ACGGCTCCTT CAGCTACTTC CCCAGCTCAG 
GAGGAGGAAA TGGAAGAAGA AGAAGAAGAG GAAGAAGGAG AAGCAGGAGA AGCAGGAGAA 
GCTGAGAGTG AGAAAGGAGG AGAGGAACTG CTCCCCCCAG AGAGTACCCC TATTCCAGCC 
AACTTGTCTC AGAATTTGGA GGCAGCAGCA GCCACTCAAG TGGCAGTATC TGTGCCAAAG 
AGGAGACGGA AAATTAAGGA GCTAAATAAG AAGGAGGCTG TTGGAGACCT TCTGGATGCC 
TTCAAGGAGG CGAACCCGGC AGTACCAGAG GTGGAAAATC AGCCTCCTGC AGGCAGCAAT 
CCAGGCCCAG AGTCTGAGGG CAGTGGTGTG CCCCCACGTC CTGAGGAAGC AGATGAGACC 
TGGGACTCAA AGGAAGACAA AATTCACAAT GCTGAGAACA TCCAGCCCGG GGAACAGAAG 
TATGAATATA AGTCAGATCA GTGGAAGCCT CTAAACCTAG AGGAGAAAAA ACGTTACGAC 
CGTGAGTTCC TGCTTGGTTT TCAGTTCATC TTTGCCAGTA TGCAGAAGCC AGAGGGATTG 
CCACATATCA GTGACGTGGT GCTGGACAAG GCCAATAAAA CACCACTGCG GCCACTGGAT 
CCCACTAGAC TACAAGGCAT AAATTGTGGC CCAGACTTCA CTCCATCCTT TGCCAACCTT 
GGCCGGACAA CCCTTAGCAC CCGTGGGCCC CCAAGGGGTG GGCCAGGTGG GGAGCTGCCC 
CGTGGGCCGG CTGGCCTGGG ACCCCGGCGC TCTCAGCAGG GACCCCGAAA AGAACCACGC 
AAGATCATTG CCACAGTGTT AATGACCGAA GATATAAAAC TGAACAAAGC AGAGAAAGCC 
TGGAAACCCA GCAGCAAGCG GACGGCGGCT GATAAGGATC GAGGGGAAGA AGATGCTGAT 
GGCAGCAAAA CCCAGGACCT ATTCCGCAGG GTGCGCTCCA TCCTGAATAA ACTGACACCC 
CAGATGTTCC AGCAGCTGAT GAAGCAAGTG ACGCAGCTGG CCATCGACAC CGAGGAACGC 
CTCAAAGGGG TCATTGACCT CATTTTTGAG AAGGCCATTT CAGAGCCCAA CTTCTCTGTG 
GCCTATGCCA ACATGTGCCG CTGCCTCATG GCGCTGAAAG TGCCCACTAC GGAAAAGCCA 
ACAGTGACTG TGAACTTCCG AAAGCTGTTG TTGAATCGAT GTCAGAAGGA GTTTGAGAAA 
GACAAAGATG ATGATGAGGT TTTTGAGAAG AAGCAAAAAG AGATGGATGA AGCTGCTACG 
GCAGAGGAAC GAGGACGCCT GAAGGAAGAG CTGGAAGAGG CTCGGGACAT AGCCCGGCGG 
CGCTCTTTAG GGAATATCAA GTTTATTGGA GAGTTGTTCA AACTGAAGAT GTTAACAGAG 
GCAATAATGC ATGACTGTGT GGTCAAACTG CTTAAGAACC ATGATGAAGA GTCCCTTGAG 
TGCCTTTGTC GTCTGCTCAC CACCATTGGC AAAGACCTGG ACTTTGAAAA AGCCAAGCCC 
CGAATGGATC AGTATTTCAA CCAGATGGAA AAAATCATTA AAGAAAAGAA GACGTCATCC 
CGCATCCGCT TTATGCTGCA GGACGTGCTG GATCTGCGAG GGAGCAATTG GGTGCCACGC 
CGAGGGGATC AGGGTCCCAA GACCATTGAC CAGATCCATA AGGAGGCTGA GATGGAAGAA 
CATCGAGAGC ACATCAAAGT GCAGCAGCTC ATGGCCAAGG GCAGTGACAA GCGTCGGGGC 
GGTCCTCCAG GCCCTCCCAT CAGCCGTGGA CTTCCCCTTG TGGATGATGG TGGCTGGAAC 
ACAGTTCCCA TCAGCAAAGG TAGCCGCCCC ATTGACACCT CACGACTCAC CAAGATCACC 
AAGCCTGGCT CCATCGATTC TAACAACCAG CTCTTTGCAC CTGGAGGGCG ACTGAGCTGG 
GGCAAGGGCA GCAGCGGAGG CTCAGGAGCC AAGCCCTCAG ACGCAGCATC AGAAGCTGCT 
CGCCCAGCTA CTAGTACTTT GAATCGCTTC TCAGCCCTTC AACAAGCGGT ACCCACAGAA 
AGCACAGATA ATAGACGTGT GGTGCAGAGG AGTAGCTTGA GCCGAGAACG AGGCGAGAAA 
GCTGGAGACC GAGGAGACCG CCTAGAGCGG AGTGAACGGG GAGGGGACCG TGGGGACCGG 
CTTGATCGTG CGCGGACACC TGCTACCAAG CGGAGCTTCA GCAAGGAAGT GGAGGAGCGG 
AGTAGAGAAC GGCCCTCCCA GCCTGAGGGG CTGCGCAAGG CAGCTAGCCT CACGGAGGAT 
CGGGACCGTG GGCGGGATGC CGTGAAGCGA GAAGCTGCCC TACCCCCAGT GAGCCCCCTG 
AAGGCGGCTC TCTCTGAGGA GGAGTTAGAG AAGAAATCCA AGGCTATCAT TGAGGAATAT 
CTCCATCTCA ATGACATGAA AGAGGCAGTC CAGTGCGTGC AGGAGCTGGC CTCACCCTCC 
TTGCTCTTCA TCTTTGTACG GCATGGTGTC GAGTCTACGC TGGAGCGCAG TGCCATTGCT 
CGTGAGCATA TGGGGCAGCT GCTGCACCAG CTGCTCTGTG CTGGGCATCT GTCTACTGCT 
CAGTACTACC AAGGGTTGTA TGAAATCTTG GAATTGGCTG AGGACATGGA AATTGACATC 
CCCCACGTGT GGCTCTACCT AGCGGAACTG GTAACACCCA TTCTGCAGGA AGGTGGGGTG 
CCCATGGGGG AGCTGTTCAG GGAGATTACA AAGCCTCTGA GACCGTTGGG CAAAGCTGCT 
TCCCTGTTGC TGGAGATCCT GGGCCTCCTG TGCAAAAGCA TGGGTCCTAA AAAGGTGGGG 
ACGCTGTGGC GAGAAGCCGG GCTTAGCTGG AAGGAATTTC TACCTGAAGG CCAGGACATT 
GGTGCATTCG TCGCTGAACA GAAGGTGGAG TATACCCTGG GAGAGGAGTC GGAAGCCCCT 
GGCCAGAGGG CACTCCCCTC CGAGGAGCTG AACAGGCAGC TGGAGAAGCT GCTGAAGGAG 
GGCAGCAGTA ACCAGCGGGT GTTCGACTGG ATAGAGGCCA ACCTGAGTGA GCAGCAGATA 
GTATCCAACA CGTTAGTTCG AGCCCTCATG ACGGCTGTCT GCTATTCTGC AATTATTTTT 
GAGACTCCCC TCCGAGTGGA CGTTGCAGTG CTGAAAGCGC GAGCGAAGCT GCTGCAGAAA 
TACCTGTGTG ACGAGCAGAA GGAGCTACAG GCGCTCTACG CCCTCCAGGC CCTTGTAGTG 
ACCTTAGAAC AGCCTCCCAA CCTGCTGCGG ATGTTCTTTG ACGCACTGTA TGACGAGGAC 
GTGGTGAAGG AGGATGCCTT CTACAGTTGG GAGAGTAGCA AGGACCCCGC TGAGCAGCAG 
GGCAAGGGTG TGGCCCTTAA ATCTGTCACA GCCTTCTTCA AGTGGCTCCG TGAAGCAGAG 
GAGGAGTCTG ACCACAACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EIF4G1 ( NM_004953.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_004944.3 Homo sapiens eukaryotic translation initiation factor 4 gamma 1 (EIF4G1), transcript variant 5, mRNA.

Price & Availability↑

CloneID	OHu13776
Clone ID Related Accession (Same CDS sequence)	NM_004953.4 , NM_004953.5
Accession Version	NM_004953.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4215bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-03-03
Organism	Homo sapiens(human)
Product	eukaryotic translation initiation factor 4 gamma 1 isoform 4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC078797.20 and BQ011010.1. On Aug 13, 2010 this sequence version replaced NM_004953.3. Transcript Variant: This variant (5) differs in the 5' UTR, lacks a portion of the 5' coding region, and uses a downstream start codon, compared to variant 1. The resulting isoform (4) is shorter at the N-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: D12686.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCTGGGG CCCGCACTGC CTCCACACCC ACCCCTCCCC AGACGGGAGG CGGTCTGGAG 
CCTCAAGCTA ATGGGGAGAC GCCCCAGGTT GCTGTCATTG TCCGGCCAGA TGACCGGTCA 
CAGGGAGCAA TCATTGCTGA CCGGCCAGGG CTGCCTGGCC CAGAGCATAG CCCTTCAGAA 
TCCCAGCCTT CGTCGCCTTC TCCGACCCCA TCACCATCCC CAGTCTTGGA ACCGGGGTCT 
GAGCCTAATC TCGCAGTCCT CTCTATTCCT GGGGACACTA TGACAACTAT ACAAATGTCT 
GTAGAAGAAT CAACCCCCAT CTCCCGTGAA ACTGGGGAGC CATATCGCCT CTCTCCAGAA 
CCCACTCCTC TCGCCGAACC CATACTGGAA GTAGAAGTGA CACTTAGCAA ACCGGTTCCA 
GAATCTGAGT TTTCTTCCAG TCCTCTCCAG GCTCCCACCC CTTTGGCATC TCACACAGTG 
GAAATTCATG AGCCTAATGG CATGGTCCCA TCTGAAGATC TGGAACCAGA GGTGGAGTCA 
AGCCCAGAGC TTGCTCCTCC CCCAGCTTGC CCCTCCGAAT CCCCTGTGCC CATTGCTCCA 
ACTGCCCAAC CTGAGGAACT GCTCAACGGA GCCCCCTCGC CACCAGCTGT GGACTTAAGC 
CCAGTCAGTG AGCCAGAGGA GCAGGCCAAG GAGGTGACAG CATCAATGGC GCCCCCCACC 
ATCCCCTCTG CTACTCCAGC TACGGCTCCT TCAGCTACTT CCCCAGCTCA GGAGGAGGAA 
ATGGAAGAAG AAGAAGAAGA GGAAGAAGGA GAAGCAGGAG AAGCAGGAGA AGCTGAGAGT 
GAGAAAGGAG GAGAGGAACT GCTCCCCCCA GAGAGTACCC CTATTCCAGC CAACTTGTCT 
CAGAATTTGG AGGCAGCAGC AGCCACTCAA GTGGCAGTAT CTGTGCCAAA GAGGAGACGG 
AAAATTAAGG AGCTAAATAA GAAGGAGGCT GTTGGAGACC TTCTGGATGC CTTCAAGGAG 
GCGAACCCGG CAGTACCAGA GGTGGAAAAT CAGCCTCCTG CAGGCAGCAA TCCAGGCCCA 
GAGTCTGAGG GCAGTGGTGT GCCCCCACGT CCTGAGGAAG CAGATGAGAC CTGGGACTCA 
AAGGAAGACA AAATTCACAA TGCTGAGAAC ATCCAGCCCG GGGAACAGAA GTATGAATAT 
AAGTCAGATC AGTGGAAGCC TCTAAACCTA GAGGAGAAAA AACGTTACGA CCGTGAGTTC 
CTGCTTGGTT TTCAGTTCAT CTTTGCCAGT ATGCAGAAGC CAGAGGGATT GCCACATATC 
AGTGACGTGG TGCTGGACAA GGCCAATAAA ACACCACTGC GGCCACTGGA TCCCACTAGA 
CTACAAGGCA TAAATTGTGG CCCAGACTTC ACTCCATCCT TTGCCAACCT TGGCCGGACA 
ACCCTTAGCA CCCGTGGGCC CCCAAGGGGT GGGCCAGGTG GGGAGCTGCC CCGTGGGCCG 
CAGGCTGGCC TGGGACCCCG GCGCTCTCAG CAGGGACCCC GAAAAGAACC ACGCAAGATC 
ATTGCCACAG TGTTAATGAC CGAAGATATA AAACTGAACA AAGCAGAGAA AGCCTGGAAA 
CCCAGCAGCA AGCGGACGGC GGCTGATAAG GATCGAGGGG AAGAAGATGC TGATGGCAGC 
AAAACCCAGG ACCTATTCCG CAGGGTGCGC TCCATCCTGA ATAAACTGAC ACCCCAGATG 
TTCCAGCAGC TGATGAAGCA AGTGACGCAG CTGGCCATCG ACACCGAGGA ACGCCTCAAA 
GGGGTCATTG ACCTCATTTT TGAGAAGGCC ATTTCAGAGC CCAACTTCTC TGTGGCCTAT 
GCCAACATGT GCCGCTGCCT CATGGCGCTG AAAGTGCCCA CTACGGAAAA GCCAACAGTG 
ACTGTGAACT TCCGAAAGCT GTTGTTGAAT CGATGTCAGA AGGAGTTTGA GAAAGACAAA 
GATGATGATG AGGTTTTTGA GAAGAAGCAA AAAGAGATGG ATGAAGCTGC TACGGCAGAG 
GAACGAGGAC GCCTGAAGGA AGAGCTGGAA GAGGCTCGGG ACATAGCCCG GCGGCGCTCT 
TTAGGGAATA TCAAGTTTAT TGGAGAGTTG TTCAAACTGA AGATGTTAAC AGAGGCAATA 
ATGCATGACT GTGTGGTCAA ACTGCTTAAG AACCATGATG AAGAGTCCCT TGAGTGCCTT 
TGTCGTCTGC TCACCACCAT TGGCAAAGAC CTGGACTTTG AAAAAGCCAA GCCCCGAATG 
GATCAGTATT TCAACCAGAT GGAAAAAATC ATTAAAGAAA AGAAGACGTC ATCCCGCATC 
CGCTTTATGC TGCAGGACGT GCTGGATCTG CGAGGGAGCA ATTGGGTGCC ACGCCGAGGG 
GATCAGGGTC CCAAGACCAT TGACCAGATC CATAAGGAGG CTGAGATGGA AGAACATCGA 
GAGCACATCA AAGTGCAGCA GCTCATGGCC AAGGGCAGTG ACAAGCGTCG GGGCGGTCCT 
CCAGGCCCTC CCATCAGCCG TGGACTTCCC CTTGTGGATG ATGGTGGCTG GAACACAGTT 
CCCATCAGCA AAGGTAGCCG CCCCATTGAC ACCTCACGAC TCACCAAGAT CACCAAGCCT 
GGCTCCATCG ATTCTAACAA CCAGCTCTTT GCACCTGGAG GGCGACTGAG CTGGGGCAAG 
GGCAGCAGCG GAGGCTCAGG AGCCAAGCCC TCAGACGCAG CATCAGAAGC TGCTCGCCCA 
GCTACTAGTA CTTTGAATCG CTTCTCAGCC CTTCAACAAG CGGTACCCAC AGAAAGCACA 
GATAATAGAC GTGTGGTGCA GAGGAGTAGC TTGAGCCGAG AACGAGGCGA GAAAGCTGGA 
GACCGAGGAG ACCGCCTAGA GCGGAGTGAA CGGGGAGGGG ACCGTGGGGA CCGGCTTGAT 
CGTGCGCGGA CACCTGCTAC CAAGCGGAGC TTCAGCAAGG AAGTGGAGGA GCGGAGTAGA 
GAACGGCCCT CCCAGCCTGA GGGGCTGCGC AAGGCAGCTA GCCTCACGGA GGATCGGGAC 
CGTGGGCGGG ATGCCGTGAA GCGAGAAGCT GCCCTACCCC CAGTGAGCCC CCTGAAGGCG 
GCTCTCTCTG AGGAGGAGTT AGAGAAGAAA TCCAAGGCTA TCATTGAGGA ATATCTCCAT 
CTCAATGACA TGAAAGAGGC AGTCCAGTGC GTGCAGGAGC TGGCCTCACC CTCCTTGCTC 
TTCATCTTTG TACGGCATGG TGTCGAGTCT ACGCTGGAGC GCAGTGCCAT TGCTCGTGAG 
CATATGGGGC AGCTGCTGCA CCAGCTGCTC TGTGCTGGGC ATCTGTCTAC TGCTCAGTAC 
TACCAAGGGT TGTATGAAAT CTTGGAATTG GCTGAGGACA TGGAAATTGA CATCCCCCAC 
GTGTGGCTCT ACCTAGCGGA ACTGGTAACA CCCATTCTGC AGGAAGGTGG GGTGCCCATG 
GGGGAGCTGT TCAGGGAGAT TACAAAGCCT CTGAGACCGT TGGGCAAAGC TGCTTCCCTG 
TTGCTGGAGA TCCTGGGCCT CCTGTGCAAA AGCATGGGTC CTAAAAAGGT GGGGACGCTG 
TGGCGAGAAG CCGGGCTTAG CTGGAAGGAA TTTCTACCTG AAGGCCAGGA CATTGGTGCA 
TTCGTCGCTG AACAGAAGGT GGAGTATACC CTGGGAGAGG AGTCGGAAGC CCCTGGCCAG 
AGGGCACTCC CCTCCGAGGA GCTGAACAGG CAGCTGGAGA AGCTGCTGAA GGAGGGCAGC 
AGTAACCAGC GGGTGTTCGA CTGGATAGAG GCCAACCTGA GTGAGCAGCA GATAGTATCC 
AACACGTTAG TTCGAGCCCT CATGACGGCT GTCTGCTATT CTGCAATTAT TTTTGAGACT 
CCCCTCCGAG TGGACGTTGC AGTGCTGAAA GCGCGAGCGA AGCTGCTGCA GAAATACCTG 
TGTGACGAGC AGAAGGAGCT ACAGGCGCTC TACGCCCTCC AGGCCCTTGT AGTGACCTTA 
GAACAGCCTC CCAACCTGCT GCGGATGTTC TTTGACGCAC TGTATGACGA GGACGTGGTG 
AAGGAGGATG CCTTCTACAG TTGGGAGAGT AGCAAGGACC CCGCTGAGCA GCAGGGCAAG 
GGTGTGGCCC TTAAATCTGT CACAGCCTTC TTCAAGTGGC TCCGTGAAGC AGAGGAGGAG 
TCTGACCACA ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004944.3
CDS	369..4583
Translation	MSGARTASTPTPPQTGGGLEPQANGETPQVAVIVRPDDRSQGAIIADRPGLPGPEHSPSESQPSSPSPTPSPSPVLEPGSEPNLAVLSIPGDTMTTIQMSVEESTPISRETGEPYRLSPEPTPLAEPILEVEVTLSKPVPESEFSSSPLQAPTPLASHTVEIHEPNGMVPSEDLEPEVESSPELAPPPACPSESPVPIAPTAQPEELLNGAPSPPAVDLSPVSEPEEQAKEVTASMAPPTIPSATPATAPSATSPAQEEEMEEEEEEEEGEAGEAGEAESEKGGEELLPPESTPIPANLSQNLEAAAATQVAVSVPKRRRKIKELNKKEAVGDLLDAFKEANPAVPEVENQPPAGSNPGPESEGSGVPPRPEEADETWDSKEDKIHNAENIQPGEQKYEYKSDQWKPLNLEEKKRYDREFLLGFQFIFASMQKPEGLPHISDVVLDKANKTPLRPLDPTRLQGINCGPDFTPSFANLGRTTLSTRGPPRGGPGGELPRGPQAGLGPRRSQQGPRKEPRKIIATVLMTEDIKLNKAEKAWKPSSKRTAADKDRGEEDADGSKTQDLFRRVRSILNKLTPQMFQQLMKQVTQLAIDTEERLKGVIDLIFEKAISEPNFSVAYANMCRCLMALKVPTTEKPTVTVNFRKLLLNRCQKEFEKDKDDDEVFEKKQKEMDEAATAEERGRLKEELEEARDIARRRSLGNIKFIGELFKLKMLTEAIMHDCVVKLLKNHDEESLECLCRLLTTIGKDLDFEKAKPRMDQYFNQMEKIIKEKKTSSRIRFMLQDVLDLRGSNWVPRRGDQGPKTIDQIHKEAEMEEHREHIKVQQLMAKGSDKRRGGPPGPPISRGLPLVDDGGWNTVPISKGSRPIDTSRLTKITKPGSIDSNNQLFAPGGRLSWGKGSSGGSGAKPSDAASEAARPATSTLNRFSALQQAVPTESTDNRRVVQRSSLSRERGEKAGDRGDRLERSERGGDRGDRLDRARTPATKRSFSKEVEERSRERPSQPEGLRKAASLTEDRDRGRDAVKREAALPPVSPLKAALSEEELEKKSKAIIEEYLHLNDMKEAVQCVQELASPSLLFIFVRHGVESTLERSAIAREHMGQLLHQLLCAGHLSTAQYYQGLYEILELAEDMEIDIPHVWLYLAELVTPILQEGGVPMGELFREITKPLRPLGKAASLLLEILGLLCKSMGPKKVGTLWREAGLSWKEFLPEGQDIGAFVAEQKVEYTLGEESEAPGQRALPSEELNRQLEKLLKEGSSNQRVFDWIEANLSEQQIVSNTLVRALMTAVCYSAIIFETPLRVDVAVLKARAKLLQKYLCDEQKELQALYALQALVVTLEQPPNLLRMFFDALYDEDVVKEDAFYSWESSKDPAEQQGKGVALKSVTAFFKWLREAEEESDHN

Target ORF information:

RefSeq Version	NM_004953.4
Organism	Homo sapiens(human)
Definition	Homo sapiens eukaryotic translation initiation factor 4 gamma 1 (EIF4G1), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004953.4

ORF Insert Sequence:

ATGTCTGGGG CCCGCACTGC CTCCACACCC ACCCCTCCCC AGACGGGAGG CGGTCTGGAG 
CCTCAAGCTA ATGGGGAGAC GCCCCAGGTT GCTGTCATTG TCCGGCCAGA TGACCGGTCA 
CAGGGAGCAA TCATTGCTGA CCGGCCAGGG CTGCCTGGCC CAGAGCATAG CCCTTCAGAA 
TCCCAGCCTT CGTCGCCTTC TCCGACCCCA TCACCATCCC CAGTCTTGGA ACCGGGGTCT 
GAGCCTAATC TCGCAGTCCT CTCTATTCCT GGGGACACTA TGACAACTAT ACAAATGTCT 
GTAGAAGAAT CAACCCCCAT CTCCCGTGAA ACTGGGGAGC CATATCGCCT CTCTCCAGAA 
CCCACTCCTC TCGCCGAACC CATACTGGAA GTAGAAGTGA CACTTAGCAA ACCGGTTCCA 
GAATCTGAGT TTTCTTCCAG TCCTCTCCAG GCTCCCACCC CTTTGGCATC TCACACAGTG 
GAAATTCATG AGCCTAATGG CATGGTCCCA TCTGAAGATC TGGAACCAGA GGTGGAGTCA 
AGCCCAGAGC TTGCTCCTCC CCCAGCTTGC CCCTCCGAAT CCCCTGTGCC CATTGCTCCA 
ACTGCCCAAC CTGAGGAACT GCTCAACGGA GCCCCCTCGC CACCAGCTGT GGACTTAAGC 
CCAGTCAGTG AGCCAGAGGA GCAGGCCAAG GAGGTGACAG CATCAATGGC GCCCCCCACC 
ATCCCCTCTG CTACTCCAGC TACGGCTCCT TCAGCTACTT CCCCAGCTCA GGAGGAGGAA 
ATGGAAGAAG AAGAAGAAGA GGAAGAAGGA GAAGCAGGAG AAGCAGGAGA AGCTGAGAGT 
GAGAAAGGAG GAGAGGAACT GCTCCCCCCA GAGAGTACCC CTATTCCAGC CAACTTGTCT 
CAGAATTTGG AGGCAGCAGC AGCCACTCAA GTGGCAGTAT CTGTGCCAAA GAGGAGACGG 
AAAATTAAGG AGCTAAATAA GAAGGAGGCT GTTGGAGACC TTCTGGATGC CTTCAAGGAG 
GCGAACCCGG CAGTACCAGA GGTGGAAAAT CAGCCTCCTG CAGGCAGCAA TCCAGGCCCA 
GAGTCTGAGG GCAGTGGTGT GCCCCCACGT CCTGAGGAAG CAGATGAGAC CTGGGACTCA 
AAGGAAGACA AAATTCACAA TGCTGAGAAC ATCCAGCCCG GGGAACAGAA GTATGAATAT 
AAGTCAGATC AGTGGAAGCC TCTAAACCTA GAGGAGAAAA AACGTTACGA CCGTGAGTTC 
CTGCTTGGTT TTCAGTTCAT CTTTGCCAGT ATGCAGAAGC CAGAGGGATT GCCACATATC 
AGTGACGTGG TGCTGGACAA GGCCAATAAA ACACCACTGC GGCCACTGGA TCCCACTAGA 
CTACAAGGCA TAAATTGTGG CCCAGACTTC ACTCCATCCT TTGCCAACCT TGGCCGGACA 
ACCCTTAGCA CCCGTGGGCC CCCAAGGGGT GGGCCAGGTG GGGAGCTGCC CCGTGGGCCG 
CAGGCTGGCC TGGGACCCCG GCGCTCTCAG CAGGGACCCC GAAAAGAACC ACGCAAGATC 
ATTGCCACAG TGTTAATGAC CGAAGATATA AAACTGAACA AAGCAGAGAA AGCCTGGAAA 
CCCAGCAGCA AGCGGACGGC GGCTGATAAG GATCGAGGGG AAGAAGATGC TGATGGCAGC 
AAAACCCAGG ACCTATTCCG CAGGGTGCGC TCCATCCTGA ATAAACTGAC ACCCCAGATG 
TTCCAGCAGC TGATGAAGCA AGTGACGCAG CTGGCCATCG ACACCGAGGA ACGCCTCAAA 
GGGGTCATTG ACCTCATTTT TGAGAAGGCC ATTTCAGAGC CCAACTTCTC TGTGGCCTAT 
GCCAACATGT GCCGCTGCCT CATGGCGCTG AAAGTGCCCA CTACGGAAAA GCCAACAGTG 
ACTGTGAACT TCCGAAAGCT GTTGTTGAAT CGATGTCAGA AGGAGTTTGA GAAAGACAAA 
GATGATGATG AGGTTTTTGA GAAGAAGCAA AAAGAGATGG ATGAAGCTGC TACGGCAGAG 
GAACGAGGAC GCCTGAAGGA AGAGCTGGAA GAGGCTCGGG ACATAGCCCG GCGGCGCTCT 
TTAGGGAATA TCAAGTTTAT TGGAGAGTTG TTCAAACTGA AGATGTTAAC AGAGGCAATA 
ATGCATGACT GTGTGGTCAA ACTGCTTAAG AACCATGATG AAGAGTCCCT TGAGTGCCTT 
TGTCGTCTGC TCACCACCAT TGGCAAAGAC CTGGACTTTG AAAAAGCCAA GCCCCGAATG 
GATCAGTATT TCAACCAGAT GGAAAAAATC ATTAAAGAAA AGAAGACGTC ATCCCGCATC 
CGCTTTATGC TGCAGGACGT GCTGGATCTG CGAGGGAGCA ATTGGGTGCC ACGCCGAGGG 
GATCAGGGTC CCAAGACCAT TGACCAGATC CATAAGGAGG CTGAGATGGA AGAACATCGA 
GAGCACATCA AAGTGCAGCA GCTCATGGCC AAGGGCAGTG ACAAGCGTCG GGGCGGTCCT 
CCAGGCCCTC CCATCAGCCG TGGACTTCCC CTTGTGGATG ATGGTGGCTG GAACACAGTT 
CCCATCAGCA AAGGTAGCCG CCCCATTGAC ACCTCACGAC TCACCAAGAT CACCAAGCCT 
GGCTCCATCG ATTCTAACAA CCAGCTCTTT GCACCTGGAG GGCGACTGAG CTGGGGCAAG 
GGCAGCAGCG GAGGCTCAGG AGCCAAGCCC TCAGACGCAG CATCAGAAGC TGCTCGCCCA 
GCTACTAGTA CTTTGAATCG CTTCTCAGCC CTTCAACAAG CGGTACCCAC AGAAAGCACA 
GATAATAGAC GTGTGGTGCA GAGGAGTAGC TTGAGCCGAG AACGAGGCGA GAAAGCTGGA 
GACCGAGGAG ACCGCCTAGA GCGGAGTGAA CGGGGAGGGG ACCGTGGGGA CCGGCTTGAT 
CGTGCGCGGA CACCTGCTAC CAAGCGGAGC TTCAGCAAGG AAGTGGAGGA GCGGAGTAGA 
GAACGGCCCT CCCAGCCTGA GGGGCTGCGC AAGGCAGCTA GCCTCACGGA GGATCGGGAC 
CGTGGGCGGG ATGCCGTGAA GCGAGAAGCT GCCCTACCCC CAGTGAGCCC CCTGAAGGCG 
GCTCTCTCTG AGGAGGAGTT AGAGAAGAAA TCCAAGGCTA TCATTGAGGA ATATCTCCAT 
CTCAATGACA TGAAAGAGGC AGTCCAGTGC GTGCAGGAGC TGGCCTCACC CTCCTTGCTC 
TTCATCTTTG TACGGCATGG TGTCGAGTCT ACGCTGGAGC GCAGTGCCAT TGCTCGTGAG 
CATATGGGGC AGCTGCTGCA CCAGCTGCTC TGTGCTGGGC ATCTGTCTAC TGCTCAGTAC 
TACCAAGGGT TGTATGAAAT CTTGGAATTG GCTGAGGACA TGGAAATTGA CATCCCCCAC 
GTGTGGCTCT ACCTAGCGGA ACTGGTAACA CCCATTCTGC AGGAAGGTGG GGTGCCCATG 
GGGGAGCTGT TCAGGGAGAT TACAAAGCCT CTGAGACCGT TGGGCAAAGC TGCTTCCCTG 
TTGCTGGAGA TCCTGGGCCT CCTGTGCAAA AGCATGGGTC CTAAAAAGGT GGGGACGCTG 
TGGCGAGAAG CCGGGCTTAG CTGGAAGGAA TTTCTACCTG AAGGCCAGGA CATTGGTGCA 
TTCGTCGCTG AACAGAAGGT GGAGTATACC CTGGGAGAGG AGTCGGAAGC CCCTGGCCAG 
AGGGCACTCC CCTCCGAGGA GCTGAACAGG CAGCTGGAGA AGCTGCTGAA GGAGGGCAGC 
AGTAACCAGC GGGTGTTCGA CTGGATAGAG GCCAACCTGA GTGAGCAGCA GATAGTATCC 
AACACGTTAG TTCGAGCCCT CATGACGGCT GTCTGCTATT CTGCAATTAT TTTTGAGACT 
CCCCTCCGAG TGGACGTTGC AGTGCTGAAA GCGCGAGCGA AGCTGCTGCA GAAATACCTG 
TGTGACGAGC AGAAGGAGCT ACAGGCGCTC TACGCCCTCC AGGCCCTTGT AGTGACCTTA 
GAACAGCCTC CCAACCTGCT GCGGATGTTC TTTGACGCAC TGTATGACGA GGACGTGGTG 
AAGGAGGATG CCTTCTACAG TTGGGAGAGT AGCAAGGACC CCGCTGAGCA GCAGGGCAAG 
GGTGTGGCCC TTAAATCTGT CACAGCCTTC TTCAAGTGGC TCCGTGAAGC AGAGGAGGAG 
TCTGACCACA ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EIF4G1 ( NM_004953.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_004944.3 Homo sapiens eukaryotic translation initiation factor 4 gamma 1 (EIF4G1), transcript variant 5, mRNA.

Price & Availability↑

CloneID	OHu13776
Clone ID Related Accession (Same CDS sequence)	NM_004953.4 , NM_004953.5
Accession Version	NM_004953.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4215bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-29
Organism	Homo sapiens(human)
Product	eukaryotic translation initiation factor 4 gamma 1 isoform 4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC078797.20 and BQ011010.1. On Jun 2, 2019 this sequence version replaced NM_004953.4. Transcript Variant: This variant (5) differs in the 5' UTR, lacks a portion of the 5' coding region, and uses a downstream start codon, compared to variant 1. The resulting isoform (4) is shorter at the N-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: D12686.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCTGGGG CCCGCACTGC CTCCACACCC ACCCCTCCCC AGACGGGAGG CGGTCTGGAG 
CCTCAAGCTA ATGGGGAGAC GCCCCAGGTT GCTGTCATTG TCCGGCCAGA TGACCGGTCA 
CAGGGAGCAA TCATTGCTGA CCGGCCAGGG CTGCCTGGCC CAGAGCATAG CCCTTCAGAA 
TCCCAGCCTT CGTCGCCTTC TCCGACCCCA TCACCATCCC CAGTCTTGGA ACCGGGGTCT 
GAGCCTAATC TCGCAGTCCT CTCTATTCCT GGGGACACTA TGACAACTAT ACAAATGTCT 
GTAGAAGAAT CAACCCCCAT CTCCCGTGAA ACTGGGGAGC CATATCGCCT CTCTCCAGAA 
CCCACTCCTC TCGCCGAACC CATACTGGAA GTAGAAGTGA CACTTAGCAA ACCGGTTCCA 
GAATCTGAGT TTTCTTCCAG TCCTCTCCAG GCTCCCACCC CTTTGGCATC TCACACAGTG 
GAAATTCATG AGCCTAATGG CATGGTCCCA TCTGAAGATC TGGAACCAGA GGTGGAGTCA 
AGCCCAGAGC TTGCTCCTCC CCCAGCTTGC CCCTCCGAAT CCCCTGTGCC CATTGCTCCA 
ACTGCCCAAC CTGAGGAACT GCTCAACGGA GCCCCCTCGC CACCAGCTGT GGACTTAAGC 
CCAGTCAGTG AGCCAGAGGA GCAGGCCAAG GAGGTGACAG CATCAATGGC GCCCCCCACC 
ATCCCCTCTG CTACTCCAGC TACGGCTCCT TCAGCTACTT CCCCAGCTCA GGAGGAGGAA 
ATGGAAGAAG AAGAAGAAGA GGAAGAAGGA GAAGCAGGAG AAGCAGGAGA AGCTGAGAGT 
GAGAAAGGAG GAGAGGAACT GCTCCCCCCA GAGAGTACCC CTATTCCAGC CAACTTGTCT 
CAGAATTTGG AGGCAGCAGC AGCCACTCAA GTGGCAGTAT CTGTGCCAAA GAGGAGACGG 
AAAATTAAGG AGCTAAATAA GAAGGAGGCT GTTGGAGACC TTCTGGATGC CTTCAAGGAG 
GCGAACCCGG CAGTACCAGA GGTGGAAAAT CAGCCTCCTG CAGGCAGCAA TCCAGGCCCA 
GAGTCTGAGG GCAGTGGTGT GCCCCCACGT CCTGAGGAAG CAGATGAGAC CTGGGACTCA 
AAGGAAGACA AAATTCACAA TGCTGAGAAC ATCCAGCCCG GGGAACAGAA GTATGAATAT 
AAGTCAGATC AGTGGAAGCC TCTAAACCTA GAGGAGAAAA AACGTTACGA CCGTGAGTTC 
CTGCTTGGTT TTCAGTTCAT CTTTGCCAGT ATGCAGAAGC CAGAGGGATT GCCACATATC 
AGTGACGTGG TGCTGGACAA GGCCAATAAA ACACCACTGC GGCCACTGGA TCCCACTAGA 
CTACAAGGCA TAAATTGTGG CCCAGACTTC ACTCCATCCT TTGCCAACCT TGGCCGGACA 
ACCCTTAGCA CCCGTGGGCC CCCAAGGGGT GGGCCAGGTG GGGAGCTGCC CCGTGGGCCG 
CAGGCTGGCC TGGGACCCCG GCGCTCTCAG CAGGGACCCC GAAAAGAACC ACGCAAGATC 
ATTGCCACAG TGTTAATGAC CGAAGATATA AAACTGAACA AAGCAGAGAA AGCCTGGAAA 
CCCAGCAGCA AGCGGACGGC GGCTGATAAG GATCGAGGGG AAGAAGATGC TGATGGCAGC 
AAAACCCAGG ACCTATTCCG CAGGGTGCGC TCCATCCTGA ATAAACTGAC ACCCCAGATG 
TTCCAGCAGC TGATGAAGCA AGTGACGCAG CTGGCCATCG ACACCGAGGA ACGCCTCAAA 
GGGGTCATTG ACCTCATTTT TGAGAAGGCC ATTTCAGAGC CCAACTTCTC TGTGGCCTAT 
GCCAACATGT GCCGCTGCCT CATGGCGCTG AAAGTGCCCA CTACGGAAAA GCCAACAGTG 
ACTGTGAACT TCCGAAAGCT GTTGTTGAAT CGATGTCAGA AGGAGTTTGA GAAAGACAAA 
GATGATGATG AGGTTTTTGA GAAGAAGCAA AAAGAGATGG ATGAAGCTGC TACGGCAGAG 
GAACGAGGAC GCCTGAAGGA AGAGCTGGAA GAGGCTCGGG ACATAGCCCG GCGGCGCTCT 
TTAGGGAATA TCAAGTTTAT TGGAGAGTTG TTCAAACTGA AGATGTTAAC AGAGGCAATA 
ATGCATGACT GTGTGGTCAA ACTGCTTAAG AACCATGATG AAGAGTCCCT TGAGTGCCTT 
TGTCGTCTGC TCACCACCAT TGGCAAAGAC CTGGACTTTG AAAAAGCCAA GCCCCGAATG 
GATCAGTATT TCAACCAGAT GGAAAAAATC ATTAAAGAAA AGAAGACGTC ATCCCGCATC 
CGCTTTATGC TGCAGGACGT GCTGGATCTG CGAGGGAGCA ATTGGGTGCC ACGCCGAGGG 
GATCAGGGTC CCAAGACCAT TGACCAGATC CATAAGGAGG CTGAGATGGA AGAACATCGA 
GAGCACATCA AAGTGCAGCA GCTCATGGCC AAGGGCAGTG ACAAGCGTCG GGGCGGTCCT 
CCAGGCCCTC CCATCAGCCG TGGACTTCCC CTTGTGGATG ATGGTGGCTG GAACACAGTT 
CCCATCAGCA AAGGTAGCCG CCCCATTGAC ACCTCACGAC TCACCAAGAT CACCAAGCCT 
GGCTCCATCG ATTCTAACAA CCAGCTCTTT GCACCTGGAG GGCGACTGAG CTGGGGCAAG 
GGCAGCAGCG GAGGCTCAGG AGCCAAGCCC TCAGACGCAG CATCAGAAGC TGCTCGCCCA 
GCTACTAGTA CTTTGAATCG CTTCTCAGCC CTTCAACAAG CGGTACCCAC AGAAAGCACA 
GATAATAGAC GTGTGGTGCA GAGGAGTAGC TTGAGCCGAG AACGAGGCGA GAAAGCTGGA 
GACCGAGGAG ACCGCCTAGA GCGGAGTGAA CGGGGAGGGG ACCGTGGGGA CCGGCTTGAT 
CGTGCGCGGA CACCTGCTAC CAAGCGGAGC TTCAGCAAGG AAGTGGAGGA GCGGAGTAGA 
GAACGGCCCT CCCAGCCTGA GGGGCTGCGC AAGGCAGCTA GCCTCACGGA GGATCGGGAC 
CGTGGGCGGG ATGCCGTGAA GCGAGAAGCT GCCCTACCCC CAGTGAGCCC CCTGAAGGCG 
GCTCTCTCTG AGGAGGAGTT AGAGAAGAAA TCCAAGGCTA TCATTGAGGA ATATCTCCAT 
CTCAATGACA TGAAAGAGGC AGTCCAGTGC GTGCAGGAGC TGGCCTCACC CTCCTTGCTC 
TTCATCTTTG TACGGCATGG TGTCGAGTCT ACGCTGGAGC GCAGTGCCAT TGCTCGTGAG 
CATATGGGGC AGCTGCTGCA CCAGCTGCTC TGTGCTGGGC ATCTGTCTAC TGCTCAGTAC 
TACCAAGGGT TGTATGAAAT CTTGGAATTG GCTGAGGACA TGGAAATTGA CATCCCCCAC 
GTGTGGCTCT ACCTAGCGGA ACTGGTAACA CCCATTCTGC AGGAAGGTGG GGTGCCCATG 
GGGGAGCTGT TCAGGGAGAT TACAAAGCCT CTGAGACCGT TGGGCAAAGC TGCTTCCCTG 
TTGCTGGAGA TCCTGGGCCT CCTGTGCAAA AGCATGGGTC CTAAAAAGGT GGGGACGCTG 
TGGCGAGAAG CCGGGCTTAG CTGGAAGGAA TTTCTACCTG AAGGCCAGGA CATTGGTGCA 
TTCGTCGCTG AACAGAAGGT GGAGTATACC CTGGGAGAGG AGTCGGAAGC CCCTGGCCAG 
AGGGCACTCC CCTCCGAGGA GCTGAACAGG CAGCTGGAGA AGCTGCTGAA GGAGGGCAGC 
AGTAACCAGC GGGTGTTCGA CTGGATAGAG GCCAACCTGA GTGAGCAGCA GATAGTATCC 
AACACGTTAG TTCGAGCCCT CATGACGGCT GTCTGCTATT CTGCAATTAT TTTTGAGACT 
CCCCTCCGAG TGGACGTTGC AGTGCTGAAA GCGCGAGCGA AGCTGCTGCA GAAATACCTG 
TGTGACGAGC AGAAGGAGCT ACAGGCGCTC TACGCCCTCC AGGCCCTTGT AGTGACCTTA 
GAACAGCCTC CCAACCTGCT GCGGATGTTC TTTGACGCAC TGTATGACGA GGACGTGGTG 
AAGGAGGATG CCTTCTACAG TTGGGAGAGT AGCAAGGACC CCGCTGAGCA GCAGGGCAAG 
GGTGTGGCCC TTAAATCTGT CACAGCCTTC TTCAAGTGGC TCCGTGAAGC AGAGGAGGAG 
TCTGACCACA ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004944.3
CDS	419..4633
Translation	MSGARTASTPTPPQTGGGLEPQANGETPQVAVIVRPDDRSQGAIIADRPGLPGPEHSPSESQPSSPSPTPSPSPVLEPGSEPNLAVLSIPGDTMTTIQMSVEESTPISRETGEPYRLSPEPTPLAEPILEVEVTLSKPVPESEFSSSPLQAPTPLASHTVEIHEPNGMVPSEDLEPEVESSPELAPPPACPSESPVPIAPTAQPEELLNGAPSPPAVDLSPVSEPEEQAKEVTASMAPPTIPSATPATAPSATSPAQEEEMEEEEEEEEGEAGEAGEAESEKGGEELLPPESTPIPANLSQNLEAAAATQVAVSVPKRRRKIKELNKKEAVGDLLDAFKEANPAVPEVENQPPAGSNPGPESEGSGVPPRPEEADETWDSKEDKIHNAENIQPGEQKYEYKSDQWKPLNLEEKKRYDREFLLGFQFIFASMQKPEGLPHISDVVLDKANKTPLRPLDPTRLQGINCGPDFTPSFANLGRTTLSTRGPPRGGPGGELPRGPQAGLGPRRSQQGPRKEPRKIIATVLMTEDIKLNKAEKAWKPSSKRTAADKDRGEEDADGSKTQDLFRRVRSILNKLTPQMFQQLMKQVTQLAIDTEERLKGVIDLIFEKAISEPNFSVAYANMCRCLMALKVPTTEKPTVTVNFRKLLLNRCQKEFEKDKDDDEVFEKKQKEMDEAATAEERGRLKEELEEARDIARRRSLGNIKFIGELFKLKMLTEAIMHDCVVKLLKNHDEESLECLCRLLTTIGKDLDFEKAKPRMDQYFNQMEKIIKEKKTSSRIRFMLQDVLDLRGSNWVPRRGDQGPKTIDQIHKEAEMEEHREHIKVQQLMAKGSDKRRGGPPGPPISRGLPLVDDGGWNTVPISKGSRPIDTSRLTKITKPGSIDSNNQLFAPGGRLSWGKGSSGGSGAKPSDAASEAARPATSTLNRFSALQQAVPTESTDNRRVVQRSSLSRERGEKAGDRGDRLERSERGGDRGDRLDRARTPATKRSFSKEVEERSRERPSQPEGLRKAASLTEDRDRGRDAVKREAALPPVSPLKAALSEEELEKKSKAIIEEYLHLNDMKEAVQCVQELASPSLLFIFVRHGVESTLERSAIAREHMGQLLHQLLCAGHLSTAQYYQGLYEILELAEDMEIDIPHVWLYLAELVTPILQEGGVPMGELFREITKPLRPLGKAASLLLEILGLLCKSMGPKKVGTLWREAGLSWKEFLPEGQDIGAFVAEQKVEYTLGEESEAPGQRALPSEELNRQLEKLLKEGSSNQRVFDWIEANLSEQQIVSNTLVRALMTAVCYSAIIFETPLRVDVAVLKARAKLLQKYLCDEQKELQALYALQALVVTLEQPPNLLRMFFDALYDEDVVKEDAFYSWESSKDPAEQQGKGVALKSVTAFFKWLREAEEESDHN

Target ORF information:

RefSeq Version	NM_004953.5
Organism	Homo sapiens(human)
Definition	Homo sapiens eukaryotic translation initiation factor 4 gamma 1 (EIF4G1), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004953.5

ORF Insert Sequence:

ATGTCTGGGG CCCGCACTGC CTCCACACCC ACCCCTCCCC AGACGGGAGG CGGTCTGGAG 
CCTCAAGCTA ATGGGGAGAC GCCCCAGGTT GCTGTCATTG TCCGGCCAGA TGACCGGTCA 
CAGGGAGCAA TCATTGCTGA CCGGCCAGGG CTGCCTGGCC CAGAGCATAG CCCTTCAGAA 
TCCCAGCCTT CGTCGCCTTC TCCGACCCCA TCACCATCCC CAGTCTTGGA ACCGGGGTCT 
GAGCCTAATC TCGCAGTCCT CTCTATTCCT GGGGACACTA TGACAACTAT ACAAATGTCT 
GTAGAAGAAT CAACCCCCAT CTCCCGTGAA ACTGGGGAGC CATATCGCCT CTCTCCAGAA 
CCCACTCCTC TCGCCGAACC CATACTGGAA GTAGAAGTGA CACTTAGCAA ACCGGTTCCA 
GAATCTGAGT TTTCTTCCAG TCCTCTCCAG GCTCCCACCC CTTTGGCATC TCACACAGTG 
GAAATTCATG AGCCTAATGG CATGGTCCCA TCTGAAGATC TGGAACCAGA GGTGGAGTCA 
AGCCCAGAGC TTGCTCCTCC CCCAGCTTGC CCCTCCGAAT CCCCTGTGCC CATTGCTCCA 
ACTGCCCAAC CTGAGGAACT GCTCAACGGA GCCCCCTCGC CACCAGCTGT GGACTTAAGC 
CCAGTCAGTG AGCCAGAGGA GCAGGCCAAG GAGGTGACAG CATCAATGGC GCCCCCCACC 
ATCCCCTCTG CTACTCCAGC TACGGCTCCT TCAGCTACTT CCCCAGCTCA GGAGGAGGAA 
ATGGAAGAAG AAGAAGAAGA GGAAGAAGGA GAAGCAGGAG AAGCAGGAGA AGCTGAGAGT 
GAGAAAGGAG GAGAGGAACT GCTCCCCCCA GAGAGTACCC CTATTCCAGC CAACTTGTCT 
CAGAATTTGG AGGCAGCAGC AGCCACTCAA GTGGCAGTAT CTGTGCCAAA GAGGAGACGG 
AAAATTAAGG AGCTAAATAA GAAGGAGGCT GTTGGAGACC TTCTGGATGC CTTCAAGGAG 
GCGAACCCGG CAGTACCAGA GGTGGAAAAT CAGCCTCCTG CAGGCAGCAA TCCAGGCCCA 
GAGTCTGAGG GCAGTGGTGT GCCCCCACGT CCTGAGGAAG CAGATGAGAC CTGGGACTCA 
AAGGAAGACA AAATTCACAA TGCTGAGAAC ATCCAGCCCG GGGAACAGAA GTATGAATAT 
AAGTCAGATC AGTGGAAGCC TCTAAACCTA GAGGAGAAAA AACGTTACGA CCGTGAGTTC 
CTGCTTGGTT TTCAGTTCAT CTTTGCCAGT ATGCAGAAGC CAGAGGGATT GCCACATATC 
AGTGACGTGG TGCTGGACAA GGCCAATAAA ACACCACTGC GGCCACTGGA TCCCACTAGA 
CTACAAGGCA TAAATTGTGG CCCAGACTTC ACTCCATCCT TTGCCAACCT TGGCCGGACA 
ACCCTTAGCA CCCGTGGGCC CCCAAGGGGT GGGCCAGGTG GGGAGCTGCC CCGTGGGCCG 
CAGGCTGGCC TGGGACCCCG GCGCTCTCAG CAGGGACCCC GAAAAGAACC ACGCAAGATC 
ATTGCCACAG TGTTAATGAC CGAAGATATA AAACTGAACA AAGCAGAGAA AGCCTGGAAA 
CCCAGCAGCA AGCGGACGGC GGCTGATAAG GATCGAGGGG AAGAAGATGC TGATGGCAGC 
AAAACCCAGG ACCTATTCCG CAGGGTGCGC TCCATCCTGA ATAAACTGAC ACCCCAGATG 
TTCCAGCAGC TGATGAAGCA AGTGACGCAG CTGGCCATCG ACACCGAGGA ACGCCTCAAA 
GGGGTCATTG ACCTCATTTT TGAGAAGGCC ATTTCAGAGC CCAACTTCTC TGTGGCCTAT 
GCCAACATGT GCCGCTGCCT CATGGCGCTG AAAGTGCCCA CTACGGAAAA GCCAACAGTG 
ACTGTGAACT TCCGAAAGCT GTTGTTGAAT CGATGTCAGA AGGAGTTTGA GAAAGACAAA 
GATGATGATG AGGTTTTTGA GAAGAAGCAA AAAGAGATGG ATGAAGCTGC TACGGCAGAG 
GAACGAGGAC GCCTGAAGGA AGAGCTGGAA GAGGCTCGGG ACATAGCCCG GCGGCGCTCT 
TTAGGGAATA TCAAGTTTAT TGGAGAGTTG TTCAAACTGA AGATGTTAAC AGAGGCAATA 
ATGCATGACT GTGTGGTCAA ACTGCTTAAG AACCATGATG AAGAGTCCCT TGAGTGCCTT 
TGTCGTCTGC TCACCACCAT TGGCAAAGAC CTGGACTTTG AAAAAGCCAA GCCCCGAATG 
GATCAGTATT TCAACCAGAT GGAAAAAATC ATTAAAGAAA AGAAGACGTC ATCCCGCATC 
CGCTTTATGC TGCAGGACGT GCTGGATCTG CGAGGGAGCA ATTGGGTGCC ACGCCGAGGG 
GATCAGGGTC CCAAGACCAT TGACCAGATC CATAAGGAGG CTGAGATGGA AGAACATCGA 
GAGCACATCA AAGTGCAGCA GCTCATGGCC AAGGGCAGTG ACAAGCGTCG GGGCGGTCCT 
CCAGGCCCTC CCATCAGCCG TGGACTTCCC CTTGTGGATG ATGGTGGCTG GAACACAGTT 
CCCATCAGCA AAGGTAGCCG CCCCATTGAC ACCTCACGAC TCACCAAGAT CACCAAGCCT 
GGCTCCATCG ATTCTAACAA CCAGCTCTTT GCACCTGGAG GGCGACTGAG CTGGGGCAAG 
GGCAGCAGCG GAGGCTCAGG AGCCAAGCCC TCAGACGCAG CATCAGAAGC TGCTCGCCCA 
GCTACTAGTA CTTTGAATCG CTTCTCAGCC CTTCAACAAG CGGTACCCAC AGAAAGCACA 
GATAATAGAC GTGTGGTGCA GAGGAGTAGC TTGAGCCGAG AACGAGGCGA GAAAGCTGGA 
GACCGAGGAG ACCGCCTAGA GCGGAGTGAA CGGGGAGGGG ACCGTGGGGA CCGGCTTGAT 
CGTGCGCGGA CACCTGCTAC CAAGCGGAGC TTCAGCAAGG AAGTGGAGGA GCGGAGTAGA 
GAACGGCCCT CCCAGCCTGA GGGGCTGCGC AAGGCAGCTA GCCTCACGGA GGATCGGGAC 
CGTGGGCGGG ATGCCGTGAA GCGAGAAGCT GCCCTACCCC CAGTGAGCCC CCTGAAGGCG 
GCTCTCTCTG AGGAGGAGTT AGAGAAGAAA TCCAAGGCTA TCATTGAGGA ATATCTCCAT 
CTCAATGACA TGAAAGAGGC AGTCCAGTGC GTGCAGGAGC TGGCCTCACC CTCCTTGCTC 
TTCATCTTTG TACGGCATGG TGTCGAGTCT ACGCTGGAGC GCAGTGCCAT TGCTCGTGAG 
CATATGGGGC AGCTGCTGCA CCAGCTGCTC TGTGCTGGGC ATCTGTCTAC TGCTCAGTAC 
TACCAAGGGT TGTATGAAAT CTTGGAATTG GCTGAGGACA TGGAAATTGA CATCCCCCAC 
GTGTGGCTCT ACCTAGCGGA ACTGGTAACA CCCATTCTGC AGGAAGGTGG GGTGCCCATG 
GGGGAGCTGT TCAGGGAGAT TACAAAGCCT CTGAGACCGT TGGGCAAAGC TGCTTCCCTG 
TTGCTGGAGA TCCTGGGCCT CCTGTGCAAA AGCATGGGTC CTAAAAAGGT GGGGACGCTG 
TGGCGAGAAG CCGGGCTTAG CTGGAAGGAA TTTCTACCTG AAGGCCAGGA CATTGGTGCA 
TTCGTCGCTG AACAGAAGGT GGAGTATACC CTGGGAGAGG AGTCGGAAGC CCCTGGCCAG 
AGGGCACTCC CCTCCGAGGA GCTGAACAGG CAGCTGGAGA AGCTGCTGAA GGAGGGCAGC 
AGTAACCAGC GGGTGTTCGA CTGGATAGAG GCCAACCTGA GTGAGCAGCA GATAGTATCC 
AACACGTTAG TTCGAGCCCT CATGACGGCT GTCTGCTATT CTGCAATTAT TTTTGAGACT 
CCCCTCCGAG TGGACGTTGC AGTGCTGAAA GCGCGAGCGA AGCTGCTGCA GAAATACCTG 
TGTGACGAGC AGAAGGAGCT ACAGGCGCTC TACGCCCTCC AGGCCCTTGT AGTGACCTTA 
GAACAGCCTC CCAACCTGCT GCGGATGTTC TTTGACGCAC TGTATGACGA GGACGTGGTG 
AAGGAGGATG CCTTCTACAG TTGGGAGAGT AGCAAGGACC CCGCTGAGCA GCAGGGCAAG 
GGTGTGGCCC TTAAATCTGT CACAGCCTTC TTCAAGTGGC TCCGTGAAGC AGAGGAGGAG 
TCTGACCACA ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EIF4G1 ( NM_198241.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_937884.1 Homo sapiens eukaryotic translation initiation factor 4 gamma 1 (EIF4G1), transcript variant 2, mRNA.

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CloneID	OHu18102
Clone ID Related Accession (Same CDS sequence)	NM_198241.2
Accession Version	NM_198241.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4800bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-03-03
Organism	Homo sapiens(human)
Product	eukaryotic translation initiation factor 4 gamma 1 isoform 5
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CR980540.1, BX647812.1, BC007788.2 and BQ011010.1. This sequence is a reference standard in the RefSeqGene project. On Aug 14, 2010 this sequence version replaced NM_198241.1. Transcript Variant: This variant (2) differs in the 5' UTR and uses an alternate in-frame splice site in the central coding region, compared to variant 1, resulting in an isoform (5) that is 1 aa shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BX647812.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAACAAAG CTCCACAGTC CACAGGCCCC CCACCCGCCC CATCCCCCGG ACTCCCACAG 
CCAGCGTTTC CCCCGGGGCA GACAGCGCCG GTGGTGTTCA GTACGCCACA AGCGACACAA 
ATGAACACGC CTTCTCAGCC CCGCCAGCAC TTCTACCCTA GCCGGGCCCA GCCCCCGAGC 
AGTGCAGCCT CCCGAGTGCA GAGTGCAGCC CCTGCCCGCC CTGGCCCAGC TGCCCATGTC 
TACCCTGCTG GATCCCAAGT AATGATGATC CCTTCCCAGA TCTCCTACCC AGCCTCCCAG 
GGGGCCTACT ACATCCCTGG ACAGGGGCGT TCCACATACG TTGTCCCGAC ACAGCAGTAC 
CCTGTGCAGC CAGGAGCCCC AGGCTTCTAT CCAGGTGCAA GCCCTACAGA ATTTGGGACC 
TACGCTGGCG CCTACTATCC AGCCCAAGGG GTGCAGCAGT TTCCCACTGG CGTGGCCCCC 
GCCCCAGTTT TGATGAACCA GCCACCCCAG ATTGCTCCCA AGAGGGAGCG TAAGACGATC 
CGAATTCGAG ATCCAAACCA AGGAGGAAAG GATATCACAG AGGAGATCAT GTCTGGGGCC 
CGCACTGCCT CCACACCCAC CCCTCCCCAG ACGGGAGGCG GTCTGGAGCC TCAAGCTAAT 
GGGGAGACGC CCCAGGTTGC TGTCATTGTC CGGCCAGATG ACCGGTCACA GGGAGCAATC 
ATTGCTGACC GGCCAGGGCT GCCTGGCCCA GAGCATAGCC CTTCAGAATC CCAGCCTTCG 
TCGCCTTCTC CGACCCCATC ACCATCCCCA GTCTTGGAAC CGGGGTCTGA GCCTAATCTC 
GCAGTCCTCT CTATTCCTGG GGACACTATG ACAACTATAC AAATGTCTGT AGAAGAATCA 
ACCCCCATCT CCCGTGAAAC TGGGGAGCCA TATCGCCTCT CTCCAGAACC CACTCCTCTC 
GCCGAACCCA TACTGGAAGT AGAAGTGACA CTTAGCAAAC CGGTTCCAGA ATCTGAGTTT 
TCTTCCAGTC CTCTCCAGGC TCCCACCCCT TTGGCATCTC ACACAGTGGA AATTCATGAG 
CCTAATGGCA TGGTCCCATC TGAAGATCTG GAACCAGAGG TGGAGTCAAG CCCAGAGCTT 
GCTCCTCCCC CAGCTTGCCC CTCCGAATCC CCTGTGCCCA TTGCTCCAAC TGCCCAACCT 
GAGGAACTGC TCAACGGAGC CCCCTCGCCA CCAGCTGTGG ACTTAAGCCC AGTCAGTGAG 
CCAGAGGAGC AGGCCAAGGA GGTGACAGCA TCAATGGCGC CCCCCACCAT CCCCTCTGCT 
ACTCCAGCTA CGGCTCCTTC AGCTACTTCC CCAGCTCAGG AGGAGGAAAT GGAAGAAGAA 
GAAGAAGAGG AAGAAGGAGA AGCAGGAGAA GCAGGAGAAG CTGAGAGTGA GAAAGGAGGA 
GAGGAACTGC TCCCCCCAGA GAGTACCCCT ATTCCAGCCA ACTTGTCTCA GAATTTGGAG 
GCAGCAGCAG CCACTCAAGT GGCAGTATCT GTGCCAAAGA GGAGACGGAA AATTAAGGAG 
CTAAATAAGA AGGAGGCTGT TGGAGACCTT CTGGATGCCT TCAAGGAGGC GAACCCGGCA 
GTACCAGAGG TGGAAAATCA GCCTCCTGCA GGCAGCAATC CAGGCCCAGA GTCTGAGGGC 
AGTGGTGTGC CCCCACGTCC TGAGGAAGCA GATGAGACCT GGGACTCAAA GGAAGACAAA 
ATTCACAATG CTGAGAACAT CCAGCCCGGG GAACAGAAGT ATGAATATAA GTCAGATCAG 
TGGAAGCCTC TAAACCTAGA GGAGAAAAAA CGTTACGACC GTGAGTTCCT GCTTGGTTTT 
CAGTTCATCT TTGCCAGTAT GCAGAAGCCA GAGGGATTGC CACATATCAG TGACGTGGTG 
CTGGACAAGG CCAATAAAAC ACCACTGCGG CCACTGGATC CCACTAGACT ACAAGGCATA 
AATTGTGGCC CAGACTTCAC TCCATCCTTT GCCAACCTTG GCCGGACAAC CCTTAGCACC 
CGTGGGCCCC CAAGGGGTGG GCCAGGTGGG GAGCTGCCCC GTGGGCCGGC TGGCCTGGGA 
CCCCGGCGCT CTCAGCAGGG ACCCCGAAAA GAACCACGCA AGATCATTGC CACAGTGTTA 
ATGACCGAAG ATATAAAACT GAACAAAGCA GAGAAAGCCT GGAAACCCAG CAGCAAGCGG 
ACGGCGGCTG ATAAGGATCG AGGGGAAGAA GATGCTGATG GCAGCAAAAC CCAGGACCTA 
TTCCGCAGGG TGCGCTCCAT CCTGAATAAA CTGACACCCC AGATGTTCCA GCAGCTGATG 
AAGCAAGTGA CGCAGCTGGC CATCGACACC GAGGAACGCC TCAAAGGGGT CATTGACCTC 
ATTTTTGAGA AGGCCATTTC AGAGCCCAAC TTCTCTGTGG CCTATGCCAA CATGTGCCGC 
TGCCTCATGG CGCTGAAAGT GCCCACTACG GAAAAGCCAA CAGTGACTGT GAACTTCCGA 
AAGCTGTTGT TGAATCGATG TCAGAAGGAG TTTGAGAAAG ACAAAGATGA TGATGAGGTT 
TTTGAGAAGA AGCAAAAAGA GATGGATGAA GCTGCTACGG CAGAGGAACG AGGACGCCTG 
AAGGAAGAGC TGGAAGAGGC TCGGGACATA GCCCGGCGGC GCTCTTTAGG GAATATCAAG 
TTTATTGGAG AGTTGTTCAA ACTGAAGATG TTAACAGAGG CAATAATGCA TGACTGTGTG 
GTCAAACTGC TTAAGAACCA TGATGAAGAG TCCCTTGAGT GCCTTTGTCG TCTGCTCACC 
ACCATTGGCA AAGACCTGGA CTTTGAAAAA GCCAAGCCCC GAATGGATCA GTATTTCAAC 
CAGATGGAAA AAATCATTAA AGAAAAGAAG ACGTCATCCC GCATCCGCTT TATGCTGCAG 
GACGTGCTGG ATCTGCGAGG GAGCAATTGG GTGCCACGCC GAGGGGATCA GGGTCCCAAG 
ACCATTGACC AGATCCATAA GGAGGCTGAG ATGGAAGAAC ATCGAGAGCA CATCAAAGTG 
CAGCAGCTCA TGGCCAAGGG CAGTGACAAG CGTCGGGGCG GTCCTCCAGG CCCTCCCATC 
AGCCGTGGAC TTCCCCTTGT GGATGATGGT GGCTGGAACA CAGTTCCCAT CAGCAAAGGT 
AGCCGCCCCA TTGACACCTC ACGACTCACC AAGATCACCA AGCCTGGCTC CATCGATTCT 
AACAACCAGC TCTTTGCACC TGGAGGGCGA CTGAGCTGGG GCAAGGGCAG CAGCGGAGGC 
TCAGGAGCCA AGCCCTCAGA CGCAGCATCA GAAGCTGCTC GCCCAGCTAC TAGTACTTTG 
AATCGCTTCT CAGCCCTTCA ACAAGCGGTA CCCACAGAAA GCACAGATAA TAGACGTGTG 
GTGCAGAGGA GTAGCTTGAG CCGAGAACGA GGCGAGAAAG CTGGAGACCG AGGAGACCGC 
CTAGAGCGGA GTGAACGGGG AGGGGACCGT GGGGACCGGC TTGATCGTGC GCGGACACCT 
GCTACCAAGC GGAGCTTCAG CAAGGAAGTG GAGGAGCGGA GTAGAGAACG GCCCTCCCAG 
CCTGAGGGGC TGCGCAAGGC AGCTAGCCTC ACGGAGGATC GGGACCGTGG GCGGGATGCC 
GTGAAGCGAG AAGCTGCCCT ACCCCCAGTG AGCCCCCTGA AGGCGGCTCT CTCTGAGGAG 
GAGTTAGAGA AGAAATCCAA GGCTATCATT GAGGAATATC TCCATCTCAA TGACATGAAA 
GAGGCAGTCC AGTGCGTGCA GGAGCTGGCC TCACCCTCCT TGCTCTTCAT CTTTGTACGG 
CATGGTGTCG AGTCTACGCT GGAGCGCAGT GCCATTGCTC GTGAGCATAT GGGGCAGCTG 
CTGCACCAGC TGCTCTGTGC TGGGCATCTG TCTACTGCTC AGTACTACCA AGGGTTGTAT 
GAAATCTTGG AATTGGCTGA GGACATGGAA ATTGACATCC CCCACGTGTG GCTCTACCTA 
GCGGAACTGG TAACACCCAT TCTGCAGGAA GGTGGGGTGC CCATGGGGGA GCTGTTCAGG 
GAGATTACAA AGCCTCTGAG ACCGTTGGGC AAAGCTGCTT CCCTGTTGCT GGAGATCCTG 
GGCCTCCTGT GCAAAAGCAT GGGTCCTAAA AAGGTGGGGA CGCTGTGGCG AGAAGCCGGG 
CTTAGCTGGA AGGAATTTCT ACCTGAAGGC CAGGACATTG GTGCATTCGT CGCTGAACAG 
AAGGTGGAGT ATACCCTGGG AGAGGAGTCG GAAGCCCCTG GCCAGAGGGC ACTCCCCTCC 
GAGGAGCTGA ACAGGCAGCT GGAGAAGCTG CTGAAGGAGG GCAGCAGTAA CCAGCGGGTG 
TTCGACTGGA TAGAGGCCAA CCTGAGTGAG CAGCAGATAG TATCCAACAC GTTAGTTCGA 
GCCCTCATGA CGGCTGTCTG CTATTCTGCA ATTATTTTTG AGACTCCCCT CCGAGTGGAC 
GTTGCAGTGC TGAAAGCGCG AGCGAAGCTG CTGCAGAAAT ACCTGTGTGA CGAGCAGAAG 
GAGCTACAGG CGCTCTACGC CCTCCAGGCC CTTGTAGTGA CCTTAGAACA GCCTCCCAAC 
CTGCTGCGGA TGTTCTTTGA CGCACTGTAT GACGAGGACG TGGTGAAGGA GGATGCCTTC 
TACAGTTGGG AGAGTAGCAA GGACCCCGCT GAGCAGCAGG GCAAGGGTGT GGCCCTTAAA 
TCTGTCACAG CCTTCTTCAA GTGGCTCCGT GAAGCAGAGG AGGAGTCTGA CCACAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_937884.1
CDS	272..5071
Translation	MNKAPQSTGPPPAPSPGLPQPAFPPGQTAPVVFSTPQATQMNTPSQPRQHFYPSRAQPPSSAASRVQSAAPARPGPAAHVYPAGSQVMMIPSQISYPASQGAYYIPGQGRSTYVVPTQQYPVQPGAPGFYPGASPTEFGTYAGAYYPAQGVQQFPTGVAPAPVLMNQPPQIAPKRERKTIRIRDPNQGGKDITEEIMSGARTASTPTPPQTGGGLEPQANGETPQVAVIVRPDDRSQGAIIADRPGLPGPEHSPSESQPSSPSPTPSPSPVLEPGSEPNLAVLSIPGDTMTTIQMSVEESTPISRETGEPYRLSPEPTPLAEPILEVEVTLSKPVPESEFSSSPLQAPTPLASHTVEIHEPNGMVPSEDLEPEVESSPELAPPPACPSESPVPIAPTAQPEELLNGAPSPPAVDLSPVSEPEEQAKEVTASMAPPTIPSATPATAPSATSPAQEEEMEEEEEEEEGEAGEAGEAESEKGGEELLPPESTPIPANLSQNLEAAAATQVAVSVPKRRRKIKELNKKEAVGDLLDAFKEANPAVPEVENQPPAGSNPGPESEGSGVPPRPEEADETWDSKEDKIHNAENIQPGEQKYEYKSDQWKPLNLEEKKRYDREFLLGFQFIFASMQKPEGLPHISDVVLDKANKTPLRPLDPTRLQGINCGPDFTPSFANLGRTTLSTRGPPRGGPGGELPRGPAGLGPRRSQQGPRKEPRKIIATVLMTEDIKLNKAEKAWKPSSKRTAADKDRGEEDADGSKTQDLFRRVRSILNKLTPQMFQQLMKQVTQLAIDTEERLKGVIDLIFEKAISEPNFSVAYANMCRCLMALKVPTTEKPTVTVNFRKLLLNRCQKEFEKDKDDDEVFEKKQKEMDEAATAEERGRLKEELEEARDIARRRSLGNIKFIGELFKLKMLTEAIMHDCVVKLLKNHDEESLECLCRLLTTIGKDLDFEKAKPRMDQYFNQMEKIIKEKKTSSRIRFMLQDVLDLRGSNWVPRRGDQGPKTIDQIHKEAEMEEHREHIKVQQLMAKGSDKRRGGPPGPPISRGLPLVDDGGWNTVPISKGSRPIDTSRLTKITKPGSIDSNNQLFAPGGRLSWGKGSSGGSGAKPSDAASEAARPATSTLNRFSALQQAVPTESTDNRRVVQRSSLSRERGEKAGDRGDRLERSERGGDRGDRLDRARTPATKRSFSKEVEERSRERPSQPEGLRKAASLTEDRDRGRDAVKREAALPPVSPLKAALSEEELEKKSKAIIEEYLHLNDMKEAVQCVQELASPSLLFIFVRHGVESTLERSAIAREHMGQLLHQLLCAGHLSTAQYYQGLYEILELAEDMEIDIPHVWLYLAELVTPILQEGGVPMGELFREITKPLRPLGKAASLLLEILGLLCKSMGPKKVGTLWREAGLSWKEFLPEGQDIGAFVAEQKVEYTLGEESEAPGQRALPSEELNRQLEKLLKEGSSNQRVFDWIEANLSEQQIVSNTLVRALMTAVCYSAIIFETPLRVDVAVLKARAKLLQKYLCDEQKELQALYALQALVVTLEQPPNLLRMFFDALYDEDVVKEDAFYSWESSKDPAEQQGKGVALKSVTAFFKWLREAEEESDHN

Target ORF information:

RefSeq Version	NM_198241.2
Organism	Homo sapiens(human)
Definition	Homo sapiens eukaryotic translation initiation factor 4 gamma 1 (EIF4G1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_198241.2

ORF Insert Sequence:

ATGAACAAAG CTCCACAGTC CACAGGCCCC CCACCCGCCC CATCCCCCGG ACTCCCACAG 
CCAGCGTTTC CCCCGGGGCA GACAGCGCCG GTGGTGTTCA GTACGCCACA AGCGACACAA 
ATGAACACGC CTTCTCAGCC CCGCCAGCAC TTCTACCCTA GCCGGGCCCA GCCCCCGAGC 
AGTGCAGCCT CCCGAGTGCA GAGTGCAGCC CCTGCCCGCC CTGGCCCAGC TGCCCATGTC 
TACCCTGCTG GATCCCAAGT AATGATGATC CCTTCCCAGA TCTCCTACCC AGCCTCCCAG 
GGGGCCTACT ACATCCCTGG ACAGGGGCGT TCCACATACG TTGTCCCGAC ACAGCAGTAC 
CCTGTGCAGC CAGGAGCCCC AGGCTTCTAT CCAGGTGCAA GCCCTACAGA ATTTGGGACC 
TACGCTGGCG CCTACTATCC AGCCCAAGGG GTGCAGCAGT TTCCCACTGG CGTGGCCCCC 
GCCCCAGTTT TGATGAACCA GCCACCCCAG ATTGCTCCCA AGAGGGAGCG TAAGACGATC 
CGAATTCGAG ATCCAAACCA AGGAGGAAAG GATATCACAG AGGAGATCAT GTCTGGGGCC 
CGCACTGCCT CCACACCCAC CCCTCCCCAG ACGGGAGGCG GTCTGGAGCC TCAAGCTAAT 
GGGGAGACGC CCCAGGTTGC TGTCATTGTC CGGCCAGATG ACCGGTCACA GGGAGCAATC 
ATTGCTGACC GGCCAGGGCT GCCTGGCCCA GAGCATAGCC CTTCAGAATC CCAGCCTTCG 
TCGCCTTCTC CGACCCCATC ACCATCCCCA GTCTTGGAAC CGGGGTCTGA GCCTAATCTC 
GCAGTCCTCT CTATTCCTGG GGACACTATG ACAACTATAC AAATGTCTGT AGAAGAATCA 
ACCCCCATCT CCCGTGAAAC TGGGGAGCCA TATCGCCTCT CTCCAGAACC CACTCCTCTC 
GCCGAACCCA TACTGGAAGT AGAAGTGACA CTTAGCAAAC CGGTTCCAGA ATCTGAGTTT 
TCTTCCAGTC CTCTCCAGGC TCCCACCCCT TTGGCATCTC ACACAGTGGA AATTCATGAG 
CCTAATGGCA TGGTCCCATC TGAAGATCTG GAACCAGAGG TGGAGTCAAG CCCAGAGCTT 
GCTCCTCCCC CAGCTTGCCC CTCCGAATCC CCTGTGCCCA TTGCTCCAAC TGCCCAACCT 
GAGGAACTGC TCAACGGAGC CCCCTCGCCA CCAGCTGTGG ACTTAAGCCC AGTCAGTGAG 
CCAGAGGAGC AGGCCAAGGA GGTGACAGCA TCAATGGCGC CCCCCACCAT CCCCTCTGCT 
ACTCCAGCTA CGGCTCCTTC AGCTACTTCC CCAGCTCAGG AGGAGGAAAT GGAAGAAGAA 
GAAGAAGAGG AAGAAGGAGA AGCAGGAGAA GCAGGAGAAG CTGAGAGTGA GAAAGGAGGA 
GAGGAACTGC TCCCCCCAGA GAGTACCCCT ATTCCAGCCA ACTTGTCTCA GAATTTGGAG 
GCAGCAGCAG CCACTCAAGT GGCAGTATCT GTGCCAAAGA GGAGACGGAA AATTAAGGAG 
CTAAATAAGA AGGAGGCTGT TGGAGACCTT CTGGATGCCT TCAAGGAGGC GAACCCGGCA 
GTACCAGAGG TGGAAAATCA GCCTCCTGCA GGCAGCAATC CAGGCCCAGA GTCTGAGGGC 
AGTGGTGTGC CCCCACGTCC TGAGGAAGCA GATGAGACCT GGGACTCAAA GGAAGACAAA 
ATTCACAATG CTGAGAACAT CCAGCCCGGG GAACAGAAGT ATGAATATAA GTCAGATCAG 
TGGAAGCCTC TAAACCTAGA GGAGAAAAAA CGTTACGACC GTGAGTTCCT GCTTGGTTTT 
CAGTTCATCT TTGCCAGTAT GCAGAAGCCA GAGGGATTGC CACATATCAG TGACGTGGTG 
CTGGACAAGG CCAATAAAAC ACCACTGCGG CCACTGGATC CCACTAGACT ACAAGGCATA 
AATTGTGGCC CAGACTTCAC TCCATCCTTT GCCAACCTTG GCCGGACAAC CCTTAGCACC 
CGTGGGCCCC CAAGGGGTGG GCCAGGTGGG GAGCTGCCCC GTGGGCCGGC TGGCCTGGGA 
CCCCGGCGCT CTCAGCAGGG ACCCCGAAAA GAACCACGCA AGATCATTGC CACAGTGTTA 
ATGACCGAAG ATATAAAACT GAACAAAGCA GAGAAAGCCT GGAAACCCAG CAGCAAGCGG 
ACGGCGGCTG ATAAGGATCG AGGGGAAGAA GATGCTGATG GCAGCAAAAC CCAGGACCTA 
TTCCGCAGGG TGCGCTCCAT CCTGAATAAA CTGACACCCC AGATGTTCCA GCAGCTGATG 
AAGCAAGTGA CGCAGCTGGC CATCGACACC GAGGAACGCC TCAAAGGGGT CATTGACCTC 
ATTTTTGAGA AGGCCATTTC AGAGCCCAAC TTCTCTGTGG CCTATGCCAA CATGTGCCGC 
TGCCTCATGG CGCTGAAAGT GCCCACTACG GAAAAGCCAA CAGTGACTGT GAACTTCCGA 
AAGCTGTTGT TGAATCGATG TCAGAAGGAG TTTGAGAAAG ACAAAGATGA TGATGAGGTT 
TTTGAGAAGA AGCAAAAAGA GATGGATGAA GCTGCTACGG CAGAGGAACG AGGACGCCTG 
AAGGAAGAGC TGGAAGAGGC TCGGGACATA GCCCGGCGGC GCTCTTTAGG GAATATCAAG 
TTTATTGGAG AGTTGTTCAA ACTGAAGATG TTAACAGAGG CAATAATGCA TGACTGTGTG 
GTCAAACTGC TTAAGAACCA TGATGAAGAG TCCCTTGAGT GCCTTTGTCG TCTGCTCACC 
ACCATTGGCA AAGACCTGGA CTTTGAAAAA GCCAAGCCCC GAATGGATCA GTATTTCAAC 
CAGATGGAAA AAATCATTAA AGAAAAGAAG ACGTCATCCC GCATCCGCTT TATGCTGCAG 
GACGTGCTGG ATCTGCGAGG GAGCAATTGG GTGCCACGCC GAGGGGATCA GGGTCCCAAG 
ACCATTGACC AGATCCATAA GGAGGCTGAG ATGGAAGAAC ATCGAGAGCA CATCAAAGTG 
CAGCAGCTCA TGGCCAAGGG CAGTGACAAG CGTCGGGGCG GTCCTCCAGG CCCTCCCATC 
AGCCGTGGAC TTCCCCTTGT GGATGATGGT GGCTGGAACA CAGTTCCCAT CAGCAAAGGT 
AGCCGCCCCA TTGACACCTC ACGACTCACC AAGATCACCA AGCCTGGCTC CATCGATTCT 
AACAACCAGC TCTTTGCACC TGGAGGGCGA CTGAGCTGGG GCAAGGGCAG CAGCGGAGGC 
TCAGGAGCCA AGCCCTCAGA CGCAGCATCA GAAGCTGCTC GCCCAGCTAC TAGTACTTTG 
AATCGCTTCT CAGCCCTTCA ACAAGCGGTA CCCACAGAAA GCACAGATAA TAGACGTGTG 
GTGCAGAGGA GTAGCTTGAG CCGAGAACGA GGCGAGAAAG CTGGAGACCG AGGAGACCGC 
CTAGAGCGGA GTGAACGGGG AGGGGACCGT GGGGACCGGC TTGATCGTGC GCGGACACCT 
GCTACCAAGC GGAGCTTCAG CAAGGAAGTG GAGGAGCGGA GTAGAGAACG GCCCTCCCAG 
CCTGAGGGGC TGCGCAAGGC AGCTAGCCTC ACGGAGGATC GGGACCGTGG GCGGGATGCC 
GTGAAGCGAG AAGCTGCCCT ACCCCCAGTG AGCCCCCTGA AGGCGGCTCT CTCTGAGGAG 
GAGTTAGAGA AGAAATCCAA GGCTATCATT GAGGAATATC TCCATCTCAA TGACATGAAA 
GAGGCAGTCC AGTGCGTGCA GGAGCTGGCC TCACCCTCCT TGCTCTTCAT CTTTGTACGG 
CATGGTGTCG AGTCTACGCT GGAGCGCAGT GCCATTGCTC GTGAGCATAT GGGGCAGCTG 
CTGCACCAGC TGCTCTGTGC TGGGCATCTG TCTACTGCTC AGTACTACCA AGGGTTGTAT 
GAAATCTTGG AATTGGCTGA GGACATGGAA ATTGACATCC CCCACGTGTG GCTCTACCTA 
GCGGAACTGG TAACACCCAT TCTGCAGGAA GGTGGGGTGC CCATGGGGGA GCTGTTCAGG 
GAGATTACAA AGCCTCTGAG ACCGTTGGGC AAAGCTGCTT CCCTGTTGCT GGAGATCCTG 
GGCCTCCTGT GCAAAAGCAT GGGTCCTAAA AAGGTGGGGA CGCTGTGGCG AGAAGCCGGG 
CTTAGCTGGA AGGAATTTCT ACCTGAAGGC CAGGACATTG GTGCATTCGT CGCTGAACAG 
AAGGTGGAGT ATACCCTGGG AGAGGAGTCG GAAGCCCCTG GCCAGAGGGC ACTCCCCTCC 
GAGGAGCTGA ACAGGCAGCT GGAGAAGCTG CTGAAGGAGG GCAGCAGTAA CCAGCGGGTG 
TTCGACTGGA TAGAGGCCAA CCTGAGTGAG CAGCAGATAG TATCCAACAC GTTAGTTCGA 
GCCCTCATGA CGGCTGTCTG CTATTCTGCA ATTATTTTTG AGACTCCCCT CCGAGTGGAC 
GTTGCAGTGC TGAAAGCGCG AGCGAAGCTG CTGCAGAAAT ACCTGTGTGA CGAGCAGAAG 
GAGCTACAGG CGCTCTACGC CCTCCAGGCC CTTGTAGTGA CCTTAGAACA GCCTCCCAAC 
CTGCTGCGGA TGTTCTTTGA CGCACTGTAT GACGAGGACG TGGTGAAGGA GGATGCCTTC 
TACAGTTGGG AGAGTAGCAA GGACCCCGCT GAGCAGCAGG GCAAGGGTGT GGCCCTTAAA 
TCTGTCACAG CCTTCTTCAA GTGGCTCCGT GAAGCAGAGG AGGAGTCTGA CCACAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EIF4G1 ( NM_001194946.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001181875.2 Homo sapiens eukaryotic translation initiation factor 4 gamma 1 (EIF4G1), transcript variant 6, mRNA.

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CloneID	OHu108646
Clone ID Related Accession (Same CDS sequence)	NM_001194946.2
Accession Version	NM_001194946.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4821bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-20
Organism	Homo sapiens(human)
Product	eukaryotic translation initiation factor 4 gamma 1 isoform 6
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC078797.20. On Jun 2, 2019 this sequence version replaced NM_001194946.1. Transcript Variant: This variant (6) differs in the 5' UTR, includes an additional in-frame exon in the 5' coding region, and uses an alternate in-frame splice site in the central coding region, compared to variant 1. The resulting isoform (6) is longer than isoform 1. Both variants 6 and 7 encode the same isoform. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC140892.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAACAAAG CTCCACAGTC CACAGGCCCC CCACCCGCCC CATCCCCCGG ACTCCCACAG 
CCAGCGTTTC CCCCGGGGCA GACAGCGCCG GTGGTGTTCA GTACGCCACA AGCGACACAA 
ATGAACACGC CTTCTCAGCC CCGCCAGGGA GGATTCAGGT CTCTGCAGCA CTTCTACCCT 
AGCCGGGCCC AGCCCCCGAG CAGTGCAGCC TCCCGAGTGC AGAGTGCAGC CCCTGCCCGC 
CCTGGCCCAG CTGCCCATGT CTACCCTGCT GGATCCCAAG TAATGATGAT CCCTTCCCAG 
ATCTCCTACC CAGCCTCCCA GGGGGCCTAC TACATCCCTG GACAGGGGCG TTCCACATAC 
GTTGTCCCGA CACAGCAGTA CCCTGTGCAG CCAGGAGCCC CAGGCTTCTA TCCAGGTGCA 
AGCCCTACAG AATTTGGGAC CTACGCTGGC GCCTACTATC CAGCCCAAGG GGTGCAGCAG 
TTTCCCACTG GCGTGGCCCC CACCCCAGTT TTGATGAACC AGCCACCCCA GATTGCTCCC 
AAGAGGGAGC GTAAGACGAT CCGAATTCGA GATCCAAACC AAGGAGGAAA GGATATCACA 
GAGGAGATCA TGTCTGGGGC CCGCACTGCC TCCACACCCA CCCCTCCCCA GACGGGAGGC 
GGTCTGGAGC CTCAAGCTAA TGGGGAGACG CCCCAGGTTG CTGTCATTGT CCGGCCAGAT 
GACCGGTCAC AGGGAGCAAT CATTGCTGAC CGGCCAGGGC TGCCTGGCCC AGAGCATAGC 
CCTTCAGAAT CCCAGCCTTC GTCGCCTTCT CCGACCCCAT CACCATCCCC AGTCTTGGAA 
CCGGGGTCTG AGCCTAATCT CGCAGTCCTC TCTATTCCTG GGGACACTAT GACAACTATA 
CAAATGTCTG TAGAAGAATC AACCCCCATC TCCCGTGAAA CTGGGGAGCC ATATCGCCTC 
TCTCCAGAAC CCACTCCTCT CGCCGAACCC ATACTGGAAG TAGAAGTGAC ACTTAGCAAA 
CCGGTTCCAG AATCTGAGTT TTCTTCCAGT CCTCTCCAGG CTCCCACCCC TTTGGCATCT 
CACACAGTGG AAATTCATGA GCCTAATGGC ATGGTCCCAT CTGAAGATCT GGAACCAGAG 
GTGGAGTCAA GCCCAGAGCT TGCTCCTCCC CCAGCTTGCC CCTCCGAATC CCCTGTGCCC 
ATTGCTCCAA CTGCCCAACC TGAGGAACTG CTCAACGGAG CCCCCTCGCC ACCAGCTGTG 
GACTTAAGCC CAGTCAGTGA GCCAGAGGAG CAGGCCAAGG AGGTGACAGC ATCAATGGCG 
CCCCCCACCA TCCCCTCTGC TACTCCAGCT ACGGCTCCTT CAGCTACTTC CCCAGCTCAG 
GAGGAGGAAA TGGAAGAAGA AGAAGAAGAG GAAGAAGGAG AAGCAGGAGA AGCAGGAGAA 
GCTGAGAGTG AGAAAGGAGG AGAGGAACTG CTCCCCCCAG AGAGTACCCC TATTCCAGCC 
AACTTGTCTC AGAATTTGGA GGCAGCAGCA GCCACTCAAG TGGCAGTATC TGTGCCAAAG 
AGGAGACGGA AAATTAAGGA GCTAAATAAG AAGGAGGCTG TTGGAGACCT TCTGGATGCC 
TTCAAGGAGG CGAACCCGGC AGTACCAGAG GTGGAAAATC AGCCTCCTGC AGGCAGCAAT 
CCAGGCCCAG AGTCTGAGGG CAGTGGTGTG CCCCCACGTC CTGAGGAAGC AGATGAGACC 
TGGGACTCAA AGGAAGACAA AATTCACAAT GCTGAGAACA TCCAGCCCGG GGAACAGAAG 
TATGAATATA AGTCAGATCA GTGGAAGCCT CTAAACCTAG AGGAGAAAAA ACGTTACGAC 
CGTGAGTTCC TGCTTGGTTT TCAGTTCATC TTTGCCAGTA TGCAGAAGCC AGAGGGATTG 
CCACATATCA GTGACGTGGT GCTGGACAAG GCCAATAAAA CACCACTGCG GCCACTGGAT 
CCCACTAGAC TACAAGGCAT AAATTGTGGC CCAGACTTCA CTCCATCCTT TGCCAACCTT 
GGCCGGACAA CCCTTAGCAC CCGTGGGCCC CCAAGGGGTG GGCCAGGTGG GGAGCTGCCC 
CGTGGGCCGG CTGGCCTGGG ACCCCGGCGC TCTCAGCAGG GACCCCGAAA AGAACCACGC 
AAGATCATTG CCACAGTGTT AATGACCGAA GATATAAAAC TGAACAAAGC AGAGAAAGCC 
TGGAAACCCA GCAGCAAGCG GACGGCGGCT GATAAGGATC GAGGGGAAGA AGATGCTGAT 
GGCAGCAAAA CCCAGGACCT ATTCCGCAGG GTGCGCTCCA TCCTGAATAA ACTGACACCC 
CAGATGTTCC AGCAGCTGAT GAAGCAAGTG ACGCAGCTGG CCATCGACAC CGAGGAACGC 
CTCAAAGGGG TCATTGACCT CATTTTTGAG AAGGCCATTT CAGAGCCCAA CTTCTCTGTG 
GCCTATGCCA ACATGTGCCG CTGCCTCATG GCGCTGAAAG TGCCCACTAC GGAAAAGCCA 
ACAGTGACTG TGAACTTCCG AAAGCTGTTG TTGAATCGAT GTCAGAAGGA GTTTGAGAAA 
GACAAAGATG ATGATGAGGT TTTTGAGAAG AAGCAAAAAG AGATGGATGA AGCTGCTACG 
GCAGAGGAAC GAGGACGCCT GAAGGAAGAG CTGGAAGAGG CTCGGGACAT AGCCCGGCGG 
CGCTCTTTAG GGAATATCAA GTTTATTGGA GAGTTGTTCA AACTGAAGAT GTTAACAGAG 
GCAATAATGC ATGACTGTGT GGTCAAACTG CTTAAGAACC ATGATGAAGA GTCCCTTGAG 
TGCCTTTGTC GTCTGCTCAC CACCATTGGC AAAGACCTGG ACTTTGAAAA AGCCAAGCCC 
CGAATGGATC AGTATTTCAA CCAGATGGAA AAAATCATTA AAGAAAAGAA GACGTCATCC 
CGCATCCGCT TTATGCTGCA GGACGTGCTG GATCTGCGAG GGAGCAATTG GGTGCCACGC 
CGAGGGGATC AGGGTCCCAA GACCATTGAC CAGATCCATA AGGAGGCTGA GATGGAAGAA 
CATCGAGAGC ACATCAAAGT GCAGCAGCTC ATGGCCAAGG GCAGTGACAA GCGTCGGGGC 
GGTCCTCCAG GCCCTCCCAT CAGCCGTGGA CTTCCCCTTG TGGATGATGG TGGCTGGAAC 
ACAGTTCCCA TCAGCAAAGG TAGCCGCCCC ATTGACACCT CACGACTCAC CAAGATCACC 
AAGCCTGGCT CCATCGATTC TAACAACCAG CTCTTTGCAC CTGGAGGGCG ACTGAGCTGG 
GGCAAGGGCA GCAGCGGAGG CTCAGGAGCC AAGCCCTCAG ACGCAGCATC AGAAGCTGCT 
CGCCCAGCTA CTAGTACTTT GAATCGCTTC TCAGCCCTTC AACAAGCGGT ACCCACAGAA 
AGCACAGATA ATAGACGTGT GGTGCAGAGG AGTAGCTTGA GCCGAGAACG AGGCGAGAAA 
GCTGGAGACC GAGGAGACCG CCTAGAGCGG AGTGAACGGG GAGGGGACCG TGGGGACCGG 
CTTGATCGTG CGCGGACACC TGCTACCAAG CGGAGCTTCA GCAAGGAAGT GGAGGAGCGG 
AGTAGAGAAC GGCCCTCCCA GCCTGAGGGG CTGCGCAAGG CAGCTAGCCT CACGGAGGAT 
CGGGACCGTG GGCGGGATGC CGTGAAGCGA GAAGCTGCCC TACCCCCAGT GAGCCCCCTG 
AAGGCGGCTC TCTCTGAGGA GGAGTTAGAG AAGAAATCCA AGGCTATCAT TGAGGAATAT 
CTCCATCTCA ATGACATGAA AGAGGCAGTC CAGTGCGTGC AGGAGCTGGC CTCACCCTCC 
TTGCTCTTCA TCTTTGTACG GCATGGTGTC GAGTCTACGC TGGAGCGCAG TGCCATTGCT 
CGTGAGCATA TGGGGCAGCT GCTGCACCAG CTGCTCTGTG CTGGGCATCT GTCTACTGCT 
CAGTACTACC AAGGGTTGTA TGAAATCTTG GAATTGGCTG AGGACATGGA AATTGACATC 
CCCCACGTGT GGCTCTACCT AGCGGAACTG GTAACACCCA TTCTGCAGGA AGGTGGGGTG 
CCCATGGGGG AGCTGTTCAG GGAGATTACA AAGCCTCTGA GACCGTTGGG CAAAGCTGCT 
TCCCTGTTGC TGGAGATCCT GGGCCTCCTG TGCAAAAGCA TGGGTCCTAA AAAGGTGGGG 
ACGCTGTGGC GAGAAGCCGG GCTTAGCTGG AAGGAATTTC TACCTGAAGG CCAGGACATT 
GGTGCATTCG TCGCTGAACA GAAGGTGGAG TATACCCTGG GAGAGGAGTC GGAAGCCCCT 
GGCCAGAGGG CACTCCCCTC CGAGGAGCTG AACAGGCAGC TGGAGAAGCT GCTGAAGGAG 
GGCAGCAGTA ACCAGCGGGT GTTCGACTGG ATAGAGGCCA ACCTGAGTGA GCAGCAGATA 
GTATCCAACA CGTTAGTTCG AGCCCTCATG ACGGCTGTCT GCTATTCTGC AATTATTTTT 
GAGACTCCCC TCCGAGTGGA CGTTGCAGTG CTGAAAGCGC GAGCGAAGCT GCTGCAGAAA 
TACCTGTGTG ACGAGCAGAA GGAGCTACAG GCGCTCTACG CCCTCCAGGC CCTTGTAGTG 
ACCTTAGAAC AGCCTCCCAA CCTGCTGCGG ATGTTCTTTG ACGCACTGTA TGACGAGGAC 
GTGGTGAAGG AGGATGCCTT CTACAGTTGG GAGAGTAGCA AGGACCCCGC TGAGCAGCAG 
GGCAAGGGTG TGGCCCTTAA ATCTGTCACA GCCTTCTTCA AGTGGCTCCG TGAAGCAGAG 
GAGGAGTCTG ACCACAACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001181875.2
CDS	161..4981
Translation	MNKAPQSTGPPPAPSPGLPQPAFPPGQTAPVVFSTPQATQMNTPSQPRQGGFRSLQHFYPSRAQPPSSAASRVQSAAPARPGPAAHVYPAGSQVMMIPSQISYPASQGAYYIPGQGRSTYVVPTQQYPVQPGAPGFYPGASPTEFGTYAGAYYPAQGVQQFPTGVAPTPVLMNQPPQIAPKRERKTIRIRDPNQGGKDITEEIMSGARTASTPTPPQTGGGLEPQANGETPQVAVIVRPDDRSQGAIIADRPGLPGPEHSPSESQPSSPSPTPSPSPVLEPGSEPNLAVLSIPGDTMTTIQMSVEESTPISRETGEPYRLSPEPTPLAEPILEVEVTLSKPVPESEFSSSPLQAPTPLASHTVEIHEPNGMVPSEDLEPEVESSPELAPPPACPSESPVPIAPTAQPEELLNGAPSPPAVDLSPVSEPEEQAKEVTASMAPPTIPSATPATAPSATSPAQEEEMEEEEEEEEGEAGEAGEAESEKGGEELLPPESTPIPANLSQNLEAAAATQVAVSVPKRRRKIKELNKKEAVGDLLDAFKEANPAVPEVENQPPAGSNPGPESEGSGVPPRPEEADETWDSKEDKIHNAENIQPGEQKYEYKSDQWKPLNLEEKKRYDREFLLGFQFIFASMQKPEGLPHISDVVLDKANKTPLRPLDPTRLQGINCGPDFTPSFANLGRTTLSTRGPPRGGPGGELPRGPAGLGPRRSQQGPRKEPRKIIATVLMTEDIKLNKAEKAWKPSSKRTAADKDRGEEDADGSKTQDLFRRVRSILNKLTPQMFQQLMKQVTQLAIDTEERLKGVIDLIFEKAISEPNFSVAYANMCRCLMALKVPTTEKPTVTVNFRKLLLNRCQKEFEKDKDDDEVFEKKQKEMDEAATAEERGRLKEELEEARDIARRRSLGNIKFIGELFKLKMLTEAIMHDCVVKLLKNHDEESLECLCRLLTTIGKDLDFEKAKPRMDQYFNQMEKIIKEKKTSSRIRFMLQDVLDLRGSNWVPRRGDQGPKTIDQIHKEAEMEEHREHIKVQQLMAKGSDKRRGGPPGPPISRGLPLVDDGGWNTVPISKGSRPIDTSRLTKITKPGSIDSNNQLFAPGGRLSWGKGSSGGSGAKPSDAASEAARPATSTLNRFSALQQAVPTESTDNRRVVQRSSLSRERGEKAGDRGDRLERSERGGDRGDRLDRARTPATKRSFSKEVEERSRERPSQPEGLRKAASLTEDRDRGRDAVKREAALPPVSPLKAALSEEELEKKSKAIIEEYLHLNDMKEAVQCVQELASPSLLFIFVRHGVESTLERSAIAREHMGQLLHQLLCAGHLSTAQYYQGLYEILELAEDMEIDIPHVWLYLAELVTPILQEGGVPMGELFREITKPLRPLGKAASLLLEILGLLCKSMGPKKVGTLWREAGLSWKEFLPEGQDIGAFVAEQKVEYTLGEESEAPGQRALPSEELNRQLEKLLKEGSSNQRVFDWIEANLSEQQIVSNTLVRALMTAVCYSAIIFETPLRVDVAVLKARAKLLQKYLCDEQKELQALYALQALVVTLEQPPNLLRMFFDALYDEDVVKEDAFYSWESSKDPAEQQGKGVALKSVTAFFKWLREAEEESDHN

Target ORF information:

RefSeq Version	NM_001194946.2
Organism	Homo sapiens(human)
Definition	Homo sapiens eukaryotic translation initiation factor 4 gamma 1 (EIF4G1), transcript variant 6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001194946.2

ORF Insert Sequence:

ATGAACAAAG CTCCACAGTC CACAGGCCCC CCACCCGCCC CATCCCCCGG ACTCCCACAG 
CCAGCGTTTC CCCCGGGGCA GACAGCGCCG GTGGTGTTCA GTACGCCACA AGCGACACAA 
ATGAACACGC CTTCTCAGCC CCGCCAGGGA GGATTCAGGT CTCTGCAGCA CTTCTACCCT 
AGCCGGGCCC AGCCCCCGAG CAGTGCAGCC TCCCGAGTGC AGAGTGCAGC CCCTGCCCGC 
CCTGGCCCAG CTGCCCATGT CTACCCTGCT GGATCCCAAG TAATGATGAT CCCTTCCCAG 
ATCTCCTACC CAGCCTCCCA GGGGGCCTAC TACATCCCTG GACAGGGGCG TTCCACATAC 
GTTGTCCCGA CACAGCAGTA CCCTGTGCAG CCAGGAGCCC CAGGCTTCTA TCCAGGTGCA 
AGCCCTACAG AATTTGGGAC CTACGCTGGC GCCTACTATC CAGCCCAAGG GGTGCAGCAG 
TTTCCCACTG GCGTGGCCCC CACCCCAGTT TTGATGAACC AGCCACCCCA GATTGCTCCC 
AAGAGGGAGC GTAAGACGAT CCGAATTCGA GATCCAAACC AAGGAGGAAA GGATATCACA 
GAGGAGATCA TGTCTGGGGC CCGCACTGCC TCCACACCCA CCCCTCCCCA GACGGGAGGC 
GGTCTGGAGC CTCAAGCTAA TGGGGAGACG CCCCAGGTTG CTGTCATTGT CCGGCCAGAT 
GACCGGTCAC AGGGAGCAAT CATTGCTGAC CGGCCAGGGC TGCCTGGCCC AGAGCATAGC 
CCTTCAGAAT CCCAGCCTTC GTCGCCTTCT CCGACCCCAT CACCATCCCC AGTCTTGGAA 
CCGGGGTCTG AGCCTAATCT CGCAGTCCTC TCTATTCCTG GGGACACTAT GACAACTATA 
CAAATGTCTG TAGAAGAATC AACCCCCATC TCCCGTGAAA CTGGGGAGCC ATATCGCCTC 
TCTCCAGAAC CCACTCCTCT CGCCGAACCC ATACTGGAAG TAGAAGTGAC ACTTAGCAAA 
CCGGTTCCAG AATCTGAGTT TTCTTCCAGT CCTCTCCAGG CTCCCACCCC TTTGGCATCT 
CACACAGTGG AAATTCATGA GCCTAATGGC ATGGTCCCAT CTGAAGATCT GGAACCAGAG 
GTGGAGTCAA GCCCAGAGCT TGCTCCTCCC CCAGCTTGCC CCTCCGAATC CCCTGTGCCC 
ATTGCTCCAA CTGCCCAACC TGAGGAACTG CTCAACGGAG CCCCCTCGCC ACCAGCTGTG 
GACTTAAGCC CAGTCAGTGA GCCAGAGGAG CAGGCCAAGG AGGTGACAGC ATCAATGGCG 
CCCCCCACCA TCCCCTCTGC TACTCCAGCT ACGGCTCCTT CAGCTACTTC CCCAGCTCAG 
GAGGAGGAAA TGGAAGAAGA AGAAGAAGAG GAAGAAGGAG AAGCAGGAGA AGCAGGAGAA 
GCTGAGAGTG AGAAAGGAGG AGAGGAACTG CTCCCCCCAG AGAGTACCCC TATTCCAGCC 
AACTTGTCTC AGAATTTGGA GGCAGCAGCA GCCACTCAAG TGGCAGTATC TGTGCCAAAG 
AGGAGACGGA AAATTAAGGA GCTAAATAAG AAGGAGGCTG TTGGAGACCT TCTGGATGCC 
TTCAAGGAGG CGAACCCGGC AGTACCAGAG GTGGAAAATC AGCCTCCTGC AGGCAGCAAT 
CCAGGCCCAG AGTCTGAGGG CAGTGGTGTG CCCCCACGTC CTGAGGAAGC AGATGAGACC 
TGGGACTCAA AGGAAGACAA AATTCACAAT GCTGAGAACA TCCAGCCCGG GGAACAGAAG 
TATGAATATA AGTCAGATCA GTGGAAGCCT CTAAACCTAG AGGAGAAAAA ACGTTACGAC 
CGTGAGTTCC TGCTTGGTTT TCAGTTCATC TTTGCCAGTA TGCAGAAGCC AGAGGGATTG 
CCACATATCA GTGACGTGGT GCTGGACAAG GCCAATAAAA CACCACTGCG GCCACTGGAT 
CCCACTAGAC TACAAGGCAT AAATTGTGGC CCAGACTTCA CTCCATCCTT TGCCAACCTT 
GGCCGGACAA CCCTTAGCAC CCGTGGGCCC CCAAGGGGTG GGCCAGGTGG GGAGCTGCCC 
CGTGGGCCGG CTGGCCTGGG ACCCCGGCGC TCTCAGCAGG GACCCCGAAA AGAACCACGC 
AAGATCATTG CCACAGTGTT AATGACCGAA GATATAAAAC TGAACAAAGC AGAGAAAGCC 
TGGAAACCCA GCAGCAAGCG GACGGCGGCT GATAAGGATC GAGGGGAAGA AGATGCTGAT 
GGCAGCAAAA CCCAGGACCT ATTCCGCAGG GTGCGCTCCA TCCTGAATAA ACTGACACCC 
CAGATGTTCC AGCAGCTGAT GAAGCAAGTG ACGCAGCTGG CCATCGACAC CGAGGAACGC 
CTCAAAGGGG TCATTGACCT CATTTTTGAG AAGGCCATTT CAGAGCCCAA CTTCTCTGTG 
GCCTATGCCA ACATGTGCCG CTGCCTCATG GCGCTGAAAG TGCCCACTAC GGAAAAGCCA 
ACAGTGACTG TGAACTTCCG AAAGCTGTTG TTGAATCGAT GTCAGAAGGA GTTTGAGAAA 
GACAAAGATG ATGATGAGGT TTTTGAGAAG AAGCAAAAAG AGATGGATGA AGCTGCTACG 
GCAGAGGAAC GAGGACGCCT GAAGGAAGAG CTGGAAGAGG CTCGGGACAT AGCCCGGCGG 
CGCTCTTTAG GGAATATCAA GTTTATTGGA GAGTTGTTCA AACTGAAGAT GTTAACAGAG 
GCAATAATGC ATGACTGTGT GGTCAAACTG CTTAAGAACC ATGATGAAGA GTCCCTTGAG 
TGCCTTTGTC GTCTGCTCAC CACCATTGGC AAAGACCTGG ACTTTGAAAA AGCCAAGCCC 
CGAATGGATC AGTATTTCAA CCAGATGGAA AAAATCATTA AAGAAAAGAA GACGTCATCC 
CGCATCCGCT TTATGCTGCA GGACGTGCTG GATCTGCGAG GGAGCAATTG GGTGCCACGC 
CGAGGGGATC AGGGTCCCAA GACCATTGAC CAGATCCATA AGGAGGCTGA GATGGAAGAA 
CATCGAGAGC ACATCAAAGT GCAGCAGCTC ATGGCCAAGG GCAGTGACAA GCGTCGGGGC 
GGTCCTCCAG GCCCTCCCAT CAGCCGTGGA CTTCCCCTTG TGGATGATGG TGGCTGGAAC 
ACAGTTCCCA TCAGCAAAGG TAGCCGCCCC ATTGACACCT CACGACTCAC CAAGATCACC 
AAGCCTGGCT CCATCGATTC TAACAACCAG CTCTTTGCAC CTGGAGGGCG ACTGAGCTGG 
GGCAAGGGCA GCAGCGGAGG CTCAGGAGCC AAGCCCTCAG ACGCAGCATC AGAAGCTGCT 
CGCCCAGCTA CTAGTACTTT GAATCGCTTC TCAGCCCTTC AACAAGCGGT ACCCACAGAA 
AGCACAGATA ATAGACGTGT GGTGCAGAGG AGTAGCTTGA GCCGAGAACG AGGCGAGAAA 
GCTGGAGACC GAGGAGACCG CCTAGAGCGG AGTGAACGGG GAGGGGACCG TGGGGACCGG 
CTTGATCGTG CGCGGACACC TGCTACCAAG CGGAGCTTCA GCAAGGAAGT GGAGGAGCGG 
AGTAGAGAAC GGCCCTCCCA GCCTGAGGGG CTGCGCAAGG CAGCTAGCCT CACGGAGGAT 
CGGGACCGTG GGCGGGATGC CGTGAAGCGA GAAGCTGCCC TACCCCCAGT GAGCCCCCTG 
AAGGCGGCTC TCTCTGAGGA GGAGTTAGAG AAGAAATCCA AGGCTATCAT TGAGGAATAT 
CTCCATCTCA ATGACATGAA AGAGGCAGTC CAGTGCGTGC AGGAGCTGGC CTCACCCTCC 
TTGCTCTTCA TCTTTGTACG GCATGGTGTC GAGTCTACGC TGGAGCGCAG TGCCATTGCT 
CGTGAGCATA TGGGGCAGCT GCTGCACCAG CTGCTCTGTG CTGGGCATCT GTCTACTGCT 
CAGTACTACC AAGGGTTGTA TGAAATCTTG GAATTGGCTG AGGACATGGA AATTGACATC 
CCCCACGTGT GGCTCTACCT AGCGGAACTG GTAACACCCA TTCTGCAGGA AGGTGGGGTG 
CCCATGGGGG AGCTGTTCAG GGAGATTACA AAGCCTCTGA GACCGTTGGG CAAAGCTGCT 
TCCCTGTTGC TGGAGATCCT GGGCCTCCTG TGCAAAAGCA TGGGTCCTAA AAAGGTGGGG 
ACGCTGTGGC GAGAAGCCGG GCTTAGCTGG AAGGAATTTC TACCTGAAGG CCAGGACATT 
GGTGCATTCG TCGCTGAACA GAAGGTGGAG TATACCCTGG GAGAGGAGTC GGAAGCCCCT 
GGCCAGAGGG CACTCCCCTC CGAGGAGCTG AACAGGCAGC TGGAGAAGCT GCTGAAGGAG 
GGCAGCAGTA ACCAGCGGGT GTTCGACTGG ATAGAGGCCA ACCTGAGTGA GCAGCAGATA 
GTATCCAACA CGTTAGTTCG AGCCCTCATG ACGGCTGTCT GCTATTCTGC AATTATTTTT 
GAGACTCCCC TCCGAGTGGA CGTTGCAGTG CTGAAAGCGC GAGCGAAGCT GCTGCAGAAA 
TACCTGTGTG ACGAGCAGAA GGAGCTACAG GCGCTCTACG CCCTCCAGGC CCTTGTAGTG 
ACCTTAGAAC AGCCTCCCAA CCTGCTGCGG ATGTTCTTTG ACGCACTGTA TGACGAGGAC 
GTGGTGAAGG AGGATGCCTT CTACAGTTGG GAGAGTAGCA AGGACCCCGC TGAGCAGCAG 
GGCAAGGGTG TGGCCCTTAA ATCTGTCACA GCCTTCTTCA AGTGGCTCCG TGAAGCAGAG 
GAGGAGTCTG ACCACAACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EIF4G1 ( NM_198241.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_937884.2 Homo sapiens eukaryotic translation initiation factor 4 gamma 1 (EIF4G1), transcript variant 2, mRNA.

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CloneID	OHu108651
Clone ID Related Accession (Same CDS sequence)	NM_198241.3
Accession Version	NM_198241.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4800bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-20
Organism	Homo sapiens(human)
Product	eukaryotic translation initiation factor 4 gamma 1 isoform 5
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC078797.20. On Jun 2, 2019 this sequence version replaced NM_198241.2. Transcript Variant: This variant (2) differs in the 5' UTR and uses an alternate in-frame splice site in the central coding region, compared to variant 1, resulting in an isoform (5) that is 1 aa shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BX647812.1, SRR7346977.2441908.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on manual assertion, conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGAACAAAG CTCCACAGTC CACAGGCCCC CCACCCGCCC CATCCCCCGG ACTCCCACAG 
CCAGCGTTTC CCCCGGGGCA GACAGCGCCG GTGGTGTTCA GTACGCCACA AGCGACACAA 
ATGAACACGC CTTCTCAGCC CCGCCAGCAC TTCTACCCTA GCCGGGCCCA GCCCCCGAGC 
AGTGCAGCCT CCCGAGTGCA GAGTGCAGCC CCTGCCCGCC CTGGCCCAGC TGCCCATGTC 
TACCCTGCTG GATCCCAAGT AATGATGATC CCTTCCCAGA TCTCCTACCC AGCCTCCCAG 
GGGGCCTACT ACATCCCTGG ACAGGGGCGT TCCACATACG TTGTCCCGAC ACAGCAGTAC 
CCTGTGCAGC CAGGAGCCCC AGGCTTCTAT CCAGGTGCAA GCCCTACAGA ATTTGGGACC 
TACGCTGGCG CCTACTATCC AGCCCAAGGG GTGCAGCAGT TTCCCACTGG CGTGGCCCCC 
ACCCCAGTTT TGATGAACCA GCCACCCCAG ATTGCTCCCA AGAGGGAGCG TAAGACGATC 
CGAATTCGAG ATCCAAACCA AGGAGGAAAG GATATCACAG AGGAGATCAT GTCTGGGGCC 
CGCACTGCCT CCACACCCAC CCCTCCCCAG ACGGGAGGCG GTCTGGAGCC TCAAGCTAAT 
GGGGAGACGC CCCAGGTTGC TGTCATTGTC CGGCCAGATG ACCGGTCACA GGGAGCAATC 
ATTGCTGACC GGCCAGGGCT GCCTGGCCCA GAGCATAGCC CTTCAGAATC CCAGCCTTCG 
TCGCCTTCTC CGACCCCATC ACCATCCCCA GTCTTGGAAC CGGGGTCTGA GCCTAATCTC 
GCAGTCCTCT CTATTCCTGG GGACACTATG ACAACTATAC AAATGTCTGT AGAAGAATCA 
ACCCCCATCT CCCGTGAAAC TGGGGAGCCA TATCGCCTCT CTCCAGAACC CACTCCTCTC 
GCCGAACCCA TACTGGAAGT AGAAGTGACA CTTAGCAAAC CGGTTCCAGA ATCTGAGTTT 
TCTTCCAGTC CTCTCCAGGC TCCCACCCCT TTGGCATCTC ACACAGTGGA AATTCATGAG 
CCTAATGGCA TGGTCCCATC TGAAGATCTG GAACCAGAGG TGGAGTCAAG CCCAGAGCTT 
GCTCCTCCCC CAGCTTGCCC CTCCGAATCC CCTGTGCCCA TTGCTCCAAC TGCCCAACCT 
GAGGAACTGC TCAACGGAGC CCCCTCGCCA CCAGCTGTGG ACTTAAGCCC AGTCAGTGAG 
CCAGAGGAGC AGGCCAAGGA GGTGACAGCA TCAATGGCGC CCCCCACCAT CCCCTCTGCT 
ACTCCAGCTA CGGCTCCTTC AGCTACTTCC CCAGCTCAGG AGGAGGAAAT GGAAGAAGAA 
GAAGAAGAGG AAGAAGGAGA AGCAGGAGAA GCAGGAGAAG CTGAGAGTGA GAAAGGAGGA 
GAGGAACTGC TCCCCCCAGA GAGTACCCCT ATTCCAGCCA ACTTGTCTCA GAATTTGGAG 
GCAGCAGCAG CCACTCAAGT GGCAGTATCT GTGCCAAAGA GGAGACGGAA AATTAAGGAG 
CTAAATAAGA AGGAGGCTGT TGGAGACCTT CTGGATGCCT TCAAGGAGGC GAACCCGGCA 
GTACCAGAGG TGGAAAATCA GCCTCCTGCA GGCAGCAATC CAGGCCCAGA GTCTGAGGGC 
AGTGGTGTGC CCCCACGTCC TGAGGAAGCA GATGAGACCT GGGACTCAAA GGAAGACAAA 
ATTCACAATG CTGAGAACAT CCAGCCCGGG GAACAGAAGT ATGAATATAA GTCAGATCAG 
TGGAAGCCTC TAAACCTAGA GGAGAAAAAA CGTTACGACC GTGAGTTCCT GCTTGGTTTT 
CAGTTCATCT TTGCCAGTAT GCAGAAGCCA GAGGGATTGC CACATATCAG TGACGTGGTG 
CTGGACAAGG CCAATAAAAC ACCACTGCGG CCACTGGATC CCACTAGACT ACAAGGCATA 
AATTGTGGCC CAGACTTCAC TCCATCCTTT GCCAACCTTG GCCGGACAAC CCTTAGCACC 
CGTGGGCCCC CAAGGGGTGG GCCAGGTGGG GAGCTGCCCC GTGGGCCGGC TGGCCTGGGA 
CCCCGGCGCT CTCAGCAGGG ACCCCGAAAA GAACCACGCA AGATCATTGC CACAGTGTTA 
ATGACCGAAG ATATAAAACT GAACAAAGCA GAGAAAGCCT GGAAACCCAG CAGCAAGCGG 
ACGGCGGCTG ATAAGGATCG AGGGGAAGAA GATGCTGATG GCAGCAAAAC CCAGGACCTA 
TTCCGCAGGG TGCGCTCCAT CCTGAATAAA CTGACACCCC AGATGTTCCA GCAGCTGATG 
AAGCAAGTGA CGCAGCTGGC CATCGACACC GAGGAACGCC TCAAAGGGGT CATTGACCTC 
ATTTTTGAGA AGGCCATTTC AGAGCCCAAC TTCTCTGTGG CCTATGCCAA CATGTGCCGC 
TGCCTCATGG CGCTGAAAGT GCCCACTACG GAAAAGCCAA CAGTGACTGT GAACTTCCGA 
AAGCTGTTGT TGAATCGATG TCAGAAGGAG TTTGAGAAAG ACAAAGATGA TGATGAGGTT 
TTTGAGAAGA AGCAAAAAGA GATGGATGAA GCTGCTACGG CAGAGGAACG AGGACGCCTG 
AAGGAAGAGC TGGAAGAGGC TCGGGACATA GCCCGGCGGC GCTCTTTAGG GAATATCAAG 
TTTATTGGAG AGTTGTTCAA ACTGAAGATG TTAACAGAGG CAATAATGCA TGACTGTGTG 
GTCAAACTGC TTAAGAACCA TGATGAAGAG TCCCTTGAGT GCCTTTGTCG TCTGCTCACC 
ACCATTGGCA AAGACCTGGA CTTTGAAAAA GCCAAGCCCC GAATGGATCA GTATTTCAAC 
CAGATGGAAA AAATCATTAA AGAAAAGAAG ACGTCATCCC GCATCCGCTT TATGCTGCAG 
GACGTGCTGG ATCTGCGAGG GAGCAATTGG GTGCCACGCC GAGGGGATCA GGGTCCCAAG 
ACCATTGACC AGATCCATAA GGAGGCTGAG ATGGAAGAAC ATCGAGAGCA CATCAAAGTG 
CAGCAGCTCA TGGCCAAGGG CAGTGACAAG CGTCGGGGCG GTCCTCCAGG CCCTCCCATC 
AGCCGTGGAC TTCCCCTTGT GGATGATGGT GGCTGGAACA CAGTTCCCAT CAGCAAAGGT 
AGCCGCCCCA TTGACACCTC ACGACTCACC AAGATCACCA AGCCTGGCTC CATCGATTCT 
AACAACCAGC TCTTTGCACC TGGAGGGCGA CTGAGCTGGG GCAAGGGCAG CAGCGGAGGC 
TCAGGAGCCA AGCCCTCAGA CGCAGCATCA GAAGCTGCTC GCCCAGCTAC TAGTACTTTG 
AATCGCTTCT CAGCCCTTCA ACAAGCGGTA CCCACAGAAA GCACAGATAA TAGACGTGTG 
GTGCAGAGGA GTAGCTTGAG CCGAGAACGA GGCGAGAAAG CTGGAGACCG AGGAGACCGC 
CTAGAGCGGA GTGAACGGGG AGGGGACCGT GGGGACCGGC TTGATCGTGC GCGGACACCT 
GCTACCAAGC GGAGCTTCAG CAAGGAAGTG GAGGAGCGGA GTAGAGAACG GCCCTCCCAG 
CCTGAGGGGC TGCGCAAGGC AGCTAGCCTC ACGGAGGATC GGGACCGTGG GCGGGATGCC 
GTGAAGCGAG AAGCTGCCCT ACCCCCAGTG AGCCCCCTGA AGGCGGCTCT CTCTGAGGAG 
GAGTTAGAGA AGAAATCCAA GGCTATCATT GAGGAATATC TCCATCTCAA TGACATGAAA 
GAGGCAGTCC AGTGCGTGCA GGAGCTGGCC TCACCCTCCT TGCTCTTCAT CTTTGTACGG 
CATGGTGTCG AGTCTACGCT GGAGCGCAGT GCCATTGCTC GTGAGCATAT GGGGCAGCTG 
CTGCACCAGC TGCTCTGTGC TGGGCATCTG TCTACTGCTC AGTACTACCA AGGGTTGTAT 
GAAATCTTGG AATTGGCTGA GGACATGGAA ATTGACATCC CCCACGTGTG GCTCTACCTA 
GCGGAACTGG TAACACCCAT TCTGCAGGAA GGTGGGGTGC CCATGGGGGA GCTGTTCAGG 
GAGATTACAA AGCCTCTGAG ACCGTTGGGC AAAGCTGCTT CCCTGTTGCT GGAGATCCTG 
GGCCTCCTGT GCAAAAGCAT GGGTCCTAAA AAGGTGGGGA CGCTGTGGCG AGAAGCCGGG 
CTTAGCTGGA AGGAATTTCT ACCTGAAGGC CAGGACATTG GTGCATTCGT CGCTGAACAG 
AAGGTGGAGT ATACCCTGGG AGAGGAGTCG GAAGCCCCTG GCCAGAGGGC ACTCCCCTCC 
GAGGAGCTGA ACAGGCAGCT GGAGAAGCTG CTGAAGGAGG GCAGCAGTAA CCAGCGGGTG 
TTCGACTGGA TAGAGGCCAA CCTGAGTGAG CAGCAGATAG TATCCAACAC GTTAGTTCGA 
GCCCTCATGA CGGCTGTCTG CTATTCTGCA ATTATTTTTG AGACTCCCCT CCGAGTGGAC 
GTTGCAGTGC TGAAAGCGCG AGCGAAGCTG CTGCAGAAAT ACCTGTGTGA CGAGCAGAAG 
GAGCTACAGG CGCTCTACGC CCTCCAGGCC CTTGTAGTGA CCTTAGAACA GCCTCCCAAC 
CTGCTGCGGA TGTTCTTTGA CGCACTGTAT GACGAGGACG TGGTGAAGGA GGATGCCTTC 
TACAGTTGGG AGAGTAGCAA GGACCCCGCT GAGCAGCAGG GCAAGGGTGT GGCCCTTAAA 
TCTGTCACAG CCTTCTTCAA GTGGCTCCGT GAAGCAGAGG AGGAGTCTGA CCACAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_937884.2
CDS	161..4960
Translation	MNKAPQSTGPPPAPSPGLPQPAFPPGQTAPVVFSTPQATQMNTPSQPRQHFYPSRAQPPSSAASRVQSAAPARPGPAAHVYPAGSQVMMIPSQISYPASQGAYYIPGQGRSTYVVPTQQYPVQPGAPGFYPGASPTEFGTYAGAYYPAQGVQQFPTGVAPTPVLMNQPPQIAPKRERKTIRIRDPNQGGKDITEEIMSGARTASTPTPPQTGGGLEPQANGETPQVAVIVRPDDRSQGAIIADRPGLPGPEHSPSESQPSSPSPTPSPSPVLEPGSEPNLAVLSIPGDTMTTIQMSVEESTPISRETGEPYRLSPEPTPLAEPILEVEVTLSKPVPESEFSSSPLQAPTPLASHTVEIHEPNGMVPSEDLEPEVESSPELAPPPACPSESPVPIAPTAQPEELLNGAPSPPAVDLSPVSEPEEQAKEVTASMAPPTIPSATPATAPSATSPAQEEEMEEEEEEEEGEAGEAGEAESEKGGEELLPPESTPIPANLSQNLEAAAATQVAVSVPKRRRKIKELNKKEAVGDLLDAFKEANPAVPEVENQPPAGSNPGPESEGSGVPPRPEEADETWDSKEDKIHNAENIQPGEQKYEYKSDQWKPLNLEEKKRYDREFLLGFQFIFASMQKPEGLPHISDVVLDKANKTPLRPLDPTRLQGINCGPDFTPSFANLGRTTLSTRGPPRGGPGGELPRGPAGLGPRRSQQGPRKEPRKIIATVLMTEDIKLNKAEKAWKPSSKRTAADKDRGEEDADGSKTQDLFRRVRSILNKLTPQMFQQLMKQVTQLAIDTEERLKGVIDLIFEKAISEPNFSVAYANMCRCLMALKVPTTEKPTVTVNFRKLLLNRCQKEFEKDKDDDEVFEKKQKEMDEAATAEERGRLKEELEEARDIARRRSLGNIKFIGELFKLKMLTEAIMHDCVVKLLKNHDEESLECLCRLLTTIGKDLDFEKAKPRMDQYFNQMEKIIKEKKTSSRIRFMLQDVLDLRGSNWVPRRGDQGPKTIDQIHKEAEMEEHREHIKVQQLMAKGSDKRRGGPPGPPISRGLPLVDDGGWNTVPISKGSRPIDTSRLTKITKPGSIDSNNQLFAPGGRLSWGKGSSGGSGAKPSDAASEAARPATSTLNRFSALQQAVPTESTDNRRVVQRSSLSRERGEKAGDRGDRLERSERGGDRGDRLDRARTPATKRSFSKEVEERSRERPSQPEGLRKAASLTEDRDRGRDAVKREAALPPVSPLKAALSEEELEKKSKAIIEEYLHLNDMKEAVQCVQELASPSLLFIFVRHGVESTLERSAIAREHMGQLLHQLLCAGHLSTAQYYQGLYEILELAEDMEIDIPHVWLYLAELVTPILQEGGVPMGELFREITKPLRPLGKAASLLLEILGLLCKSMGPKKVGTLWREAGLSWKEFLPEGQDIGAFVAEQKVEYTLGEESEAPGQRALPSEELNRQLEKLLKEGSSNQRVFDWIEANLSEQQIVSNTLVRALMTAVCYSAIIFETPLRVDVAVLKARAKLLQKYLCDEQKELQALYALQALVVTLEQPPNLLRMFFDALYDEDVVKEDAFYSWESSKDPAEQQGKGVALKSVTAFFKWLREAEEESDHN

Target ORF information:

RefSeq Version	NM_198241.3
Organism	Homo sapiens(human)
Definition	Homo sapiens eukaryotic translation initiation factor 4 gamma 1 (EIF4G1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_198241.3

ORF Insert Sequence:

ATGAACAAAG CTCCACAGTC CACAGGCCCC CCACCCGCCC CATCCCCCGG ACTCCCACAG 
CCAGCGTTTC CCCCGGGGCA GACAGCGCCG GTGGTGTTCA GTACGCCACA AGCGACACAA 
ATGAACACGC CTTCTCAGCC CCGCCAGCAC TTCTACCCTA GCCGGGCCCA GCCCCCGAGC 
AGTGCAGCCT CCCGAGTGCA GAGTGCAGCC CCTGCCCGCC CTGGCCCAGC TGCCCATGTC 
TACCCTGCTG GATCCCAAGT AATGATGATC CCTTCCCAGA TCTCCTACCC AGCCTCCCAG 
GGGGCCTACT ACATCCCTGG ACAGGGGCGT TCCACATACG TTGTCCCGAC ACAGCAGTAC 
CCTGTGCAGC CAGGAGCCCC AGGCTTCTAT CCAGGTGCAA GCCCTACAGA ATTTGGGACC 
TACGCTGGCG CCTACTATCC AGCCCAAGGG GTGCAGCAGT TTCCCACTGG CGTGGCCCCC 
ACCCCAGTTT TGATGAACCA GCCACCCCAG ATTGCTCCCA AGAGGGAGCG TAAGACGATC 
CGAATTCGAG ATCCAAACCA AGGAGGAAAG GATATCACAG AGGAGATCAT GTCTGGGGCC 
CGCACTGCCT CCACACCCAC CCCTCCCCAG ACGGGAGGCG GTCTGGAGCC TCAAGCTAAT 
GGGGAGACGC CCCAGGTTGC TGTCATTGTC CGGCCAGATG ACCGGTCACA GGGAGCAATC 
ATTGCTGACC GGCCAGGGCT GCCTGGCCCA GAGCATAGCC CTTCAGAATC CCAGCCTTCG 
TCGCCTTCTC CGACCCCATC ACCATCCCCA GTCTTGGAAC CGGGGTCTGA GCCTAATCTC 
GCAGTCCTCT CTATTCCTGG GGACACTATG ACAACTATAC AAATGTCTGT AGAAGAATCA 
ACCCCCATCT CCCGTGAAAC TGGGGAGCCA TATCGCCTCT CTCCAGAACC CACTCCTCTC 
GCCGAACCCA TACTGGAAGT AGAAGTGACA CTTAGCAAAC CGGTTCCAGA ATCTGAGTTT 
TCTTCCAGTC CTCTCCAGGC TCCCACCCCT TTGGCATCTC ACACAGTGGA AATTCATGAG 
CCTAATGGCA TGGTCCCATC TGAAGATCTG GAACCAGAGG TGGAGTCAAG CCCAGAGCTT 
GCTCCTCCCC CAGCTTGCCC CTCCGAATCC CCTGTGCCCA TTGCTCCAAC TGCCCAACCT 
GAGGAACTGC TCAACGGAGC CCCCTCGCCA CCAGCTGTGG ACTTAAGCCC AGTCAGTGAG 
CCAGAGGAGC AGGCCAAGGA GGTGACAGCA TCAATGGCGC CCCCCACCAT CCCCTCTGCT 
ACTCCAGCTA CGGCTCCTTC AGCTACTTCC CCAGCTCAGG AGGAGGAAAT GGAAGAAGAA 
GAAGAAGAGG AAGAAGGAGA AGCAGGAGAA GCAGGAGAAG CTGAGAGTGA GAAAGGAGGA 
GAGGAACTGC TCCCCCCAGA GAGTACCCCT ATTCCAGCCA ACTTGTCTCA GAATTTGGAG 
GCAGCAGCAG CCACTCAAGT GGCAGTATCT GTGCCAAAGA GGAGACGGAA AATTAAGGAG 
CTAAATAAGA AGGAGGCTGT TGGAGACCTT CTGGATGCCT TCAAGGAGGC GAACCCGGCA 
GTACCAGAGG TGGAAAATCA GCCTCCTGCA GGCAGCAATC CAGGCCCAGA GTCTGAGGGC 
AGTGGTGTGC CCCCACGTCC TGAGGAAGCA GATGAGACCT GGGACTCAAA GGAAGACAAA 
ATTCACAATG CTGAGAACAT CCAGCCCGGG GAACAGAAGT ATGAATATAA GTCAGATCAG 
TGGAAGCCTC TAAACCTAGA GGAGAAAAAA CGTTACGACC GTGAGTTCCT GCTTGGTTTT 
CAGTTCATCT TTGCCAGTAT GCAGAAGCCA GAGGGATTGC CACATATCAG TGACGTGGTG 
CTGGACAAGG CCAATAAAAC ACCACTGCGG CCACTGGATC CCACTAGACT ACAAGGCATA 
AATTGTGGCC CAGACTTCAC TCCATCCTTT GCCAACCTTG GCCGGACAAC CCTTAGCACC 
CGTGGGCCCC CAAGGGGTGG GCCAGGTGGG GAGCTGCCCC GTGGGCCGGC TGGCCTGGGA 
CCCCGGCGCT CTCAGCAGGG ACCCCGAAAA GAACCACGCA AGATCATTGC CACAGTGTTA 
ATGACCGAAG ATATAAAACT GAACAAAGCA GAGAAAGCCT GGAAACCCAG CAGCAAGCGG 
ACGGCGGCTG ATAAGGATCG AGGGGAAGAA GATGCTGATG GCAGCAAAAC CCAGGACCTA 
TTCCGCAGGG TGCGCTCCAT CCTGAATAAA CTGACACCCC AGATGTTCCA GCAGCTGATG 
AAGCAAGTGA CGCAGCTGGC CATCGACACC GAGGAACGCC TCAAAGGGGT CATTGACCTC 
ATTTTTGAGA AGGCCATTTC AGAGCCCAAC TTCTCTGTGG CCTATGCCAA CATGTGCCGC 
TGCCTCATGG CGCTGAAAGT GCCCACTACG GAAAAGCCAA CAGTGACTGT GAACTTCCGA 
AAGCTGTTGT TGAATCGATG TCAGAAGGAG TTTGAGAAAG ACAAAGATGA TGATGAGGTT 
TTTGAGAAGA AGCAAAAAGA GATGGATGAA GCTGCTACGG CAGAGGAACG AGGACGCCTG 
AAGGAAGAGC TGGAAGAGGC TCGGGACATA GCCCGGCGGC GCTCTTTAGG GAATATCAAG 
TTTATTGGAG AGTTGTTCAA ACTGAAGATG TTAACAGAGG CAATAATGCA TGACTGTGTG 
GTCAAACTGC TTAAGAACCA TGATGAAGAG TCCCTTGAGT GCCTTTGTCG TCTGCTCACC 
ACCATTGGCA AAGACCTGGA CTTTGAAAAA GCCAAGCCCC GAATGGATCA GTATTTCAAC 
CAGATGGAAA AAATCATTAA AGAAAAGAAG ACGTCATCCC GCATCCGCTT TATGCTGCAG 
GACGTGCTGG ATCTGCGAGG GAGCAATTGG GTGCCACGCC GAGGGGATCA GGGTCCCAAG 
ACCATTGACC AGATCCATAA GGAGGCTGAG ATGGAAGAAC ATCGAGAGCA CATCAAAGTG 
CAGCAGCTCA TGGCCAAGGG CAGTGACAAG CGTCGGGGCG GTCCTCCAGG CCCTCCCATC 
AGCCGTGGAC TTCCCCTTGT GGATGATGGT GGCTGGAACA CAGTTCCCAT CAGCAAAGGT 
AGCCGCCCCA TTGACACCTC ACGACTCACC AAGATCACCA AGCCTGGCTC CATCGATTCT 
AACAACCAGC TCTTTGCACC TGGAGGGCGA CTGAGCTGGG GCAAGGGCAG CAGCGGAGGC 
TCAGGAGCCA AGCCCTCAGA CGCAGCATCA GAAGCTGCTC GCCCAGCTAC TAGTACTTTG 
AATCGCTTCT CAGCCCTTCA ACAAGCGGTA CCCACAGAAA GCACAGATAA TAGACGTGTG 
GTGCAGAGGA GTAGCTTGAG CCGAGAACGA GGCGAGAAAG CTGGAGACCG AGGAGACCGC 
CTAGAGCGGA GTGAACGGGG AGGGGACCGT GGGGACCGGC TTGATCGTGC GCGGACACCT 
GCTACCAAGC GGAGCTTCAG CAAGGAAGTG GAGGAGCGGA GTAGAGAACG GCCCTCCCAG 
CCTGAGGGGC TGCGCAAGGC AGCTAGCCTC ACGGAGGATC GGGACCGTGG GCGGGATGCC 
GTGAAGCGAG AAGCTGCCCT ACCCCCAGTG AGCCCCCTGA AGGCGGCTCT CTCTGAGGAG 
GAGTTAGAGA AGAAATCCAA GGCTATCATT GAGGAATATC TCCATCTCAA TGACATGAAA 
GAGGCAGTCC AGTGCGTGCA GGAGCTGGCC TCACCCTCCT TGCTCTTCAT CTTTGTACGG 
CATGGTGTCG AGTCTACGCT GGAGCGCAGT GCCATTGCTC GTGAGCATAT GGGGCAGCTG 
CTGCACCAGC TGCTCTGTGC TGGGCATCTG TCTACTGCTC AGTACTACCA AGGGTTGTAT 
GAAATCTTGG AATTGGCTGA GGACATGGAA ATTGACATCC CCCACGTGTG GCTCTACCTA 
GCGGAACTGG TAACACCCAT TCTGCAGGAA GGTGGGGTGC CCATGGGGGA GCTGTTCAGG 
GAGATTACAA AGCCTCTGAG ACCGTTGGGC AAAGCTGCTT CCCTGTTGCT GGAGATCCTG 
GGCCTCCTGT GCAAAAGCAT GGGTCCTAAA AAGGTGGGGA CGCTGTGGCG AGAAGCCGGG 
CTTAGCTGGA AGGAATTTCT ACCTGAAGGC CAGGACATTG GTGCATTCGT CGCTGAACAG 
AAGGTGGAGT ATACCCTGGG AGAGGAGTCG GAAGCCCCTG GCCAGAGGGC ACTCCCCTCC 
GAGGAGCTGA ACAGGCAGCT GGAGAAGCTG CTGAAGGAGG GCAGCAGTAA CCAGCGGGTG 
TTCGACTGGA TAGAGGCCAA CCTGAGTGAG CAGCAGATAG TATCCAACAC GTTAGTTCGA 
GCCCTCATGA CGGCTGTCTG CTATTCTGCA ATTATTTTTG AGACTCCCCT CCGAGTGGAC 
GTTGCAGTGC TGAAAGCGCG AGCGAAGCTG CTGCAGAAAT ACCTGTGTGA CGAGCAGAAG 
GAGCTACAGG CGCTCTACGC CCTCCAGGCC CTTGTAGTGA CCTTAGAACA GCCTCCCAAC 
CTGCTGCGGA TGTTCTTTGA CGCACTGTAT GACGAGGACG TGGTGAAGGA GGATGCCTTC 
TACAGTTGGG AGAGTAGCAA GGACCCCGCT GAGCAGCAGG GCAAGGGTGT GGCCCTTAAA 
TCTGTCACAG CCTTCTTCAA GTGGCTCCGT GAAGCAGAGG AGGAGTCTGA CCACAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EIF4G1 ( NM_001291157.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001278086.2 Homo sapiens eukaryotic translation initiation factor 4 gamma 1 (EIF4G1), transcript variant 8, mRNA.

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CloneID	OHu108653
Clone ID Related Accession (Same CDS sequence)	NM_001291157.2
Accession Version	NM_001291157.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4680bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-29
Organism	Homo sapiens(human)
Product	eukaryotic translation initiation factor 4 gamma 1 isoform 7
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC078797.20. On Jun 2, 2019 this sequence version replaced NM_001291157.1. Transcript Variant: This variant (8) differs in the 5' UTR, includes an additional internal segment in the 5' region, which results in a downstream start codon, and uses an alternate in-frame splice site in the central coding region, compared to variant 1. The resulting isoform (7) is shorter at the N-terminus, and lacks an internal residue, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK226160.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END##

ATGAACACGC CTTCTCAGCC CCGCCAGCAC TTCTACCCTA GCCGGGCCCA GCCCCCGAGC 
AGTGCAGCCT CCCGAGTGCA GAGTGCAGCC CCTGCCCGCC CTGGCCCAGC TGCCCATGTC 
TACCCTGCTG GATCCCAAGT AATGATGATC CCTTCCCAGA TCTCCTACCC AGCCTCCCAG 
GGGGCCTACT ACATCCCTGG ACAGGGGCGT TCCACATACG TTGTCCCGAC ACAGCAGTAC 
CCTGTGCAGC CAGGAGCCCC AGGCTTCTAT CCAGGTGCAA GCCCTACAGA ATTTGGGACC 
TACGCTGGCG CCTACTATCC AGCCCAAGGG GTGCAGCAGT TTCCCACTGG CGTGGCCCCC 
ACCCCAGTTT TGATGAACCA GCCACCCCAG ATTGCTCCCA AGAGGGAGCG TAAGACGATC 
CGAATTCGAG ATCCAAACCA AGGAGGAAAG GATATCACAG AGGAGATCAT GTCTGGGGCC 
CGCACTGCCT CCACACCCAC CCCTCCCCAG ACGGGAGGCG GTCTGGAGCC TCAAGCTAAT 
GGGGAGACGC CCCAGGTTGC TGTCATTGTC CGGCCAGATG ACCGGTCACA GGGAGCAATC 
ATTGCTGACC GGCCAGGGCT GCCTGGCCCA GAGCATAGCC CTTCAGAATC CCAGCCTTCG 
TCGCCTTCTC CGACCCCATC ACCATCCCCA GTCTTGGAAC CGGGGTCTGA GCCTAATCTC 
GCAGTCCTCT CTATTCCTGG GGACACTATG ACAACTATAC AAATGTCTGT AGAAGAATCA 
ACCCCCATCT CCCGTGAAAC TGGGGAGCCA TATCGCCTCT CTCCAGAACC CACTCCTCTC 
GCCGAACCCA TACTGGAAGT AGAAGTGACA CTTAGCAAAC CGGTTCCAGA ATCTGAGTTT 
TCTTCCAGTC CTCTCCAGGC TCCCACCCCT TTGGCATCTC ACACAGTGGA AATTCATGAG 
CCTAATGGCA TGGTCCCATC TGAAGATCTG GAACCAGAGG TGGAGTCAAG CCCAGAGCTT 
GCTCCTCCCC CAGCTTGCCC CTCCGAATCC CCTGTGCCCA TTGCTCCAAC TGCCCAACCT 
GAGGAACTGC TCAACGGAGC CCCCTCGCCA CCAGCTGTGG ACTTAAGCCC AGTCAGTGAG 
CCAGAGGAGC AGGCCAAGGA GGTGACAGCA TCAATGGCGC CCCCCACCAT CCCCTCTGCT 
ACTCCAGCTA CGGCTCCTTC AGCTACTTCC CCAGCTCAGG AGGAGGAAAT GGAAGAAGAA 
GAAGAAGAGG AAGAAGGAGA AGCAGGAGAA GCAGGAGAAG CTGAGAGTGA GAAAGGAGGA 
GAGGAACTGC TCCCCCCAGA GAGTACCCCT ATTCCAGCCA ACTTGTCTCA GAATTTGGAG 
GCAGCAGCAG CCACTCAAGT GGCAGTATCT GTGCCAAAGA GGAGACGGAA AATTAAGGAG 
CTAAATAAGA AGGAGGCTGT TGGAGACCTT CTGGATGCCT TCAAGGAGGC GAACCCGGCA 
GTACCAGAGG TGGAAAATCA GCCTCCTGCA GGCAGCAATC CAGGCCCAGA GTCTGAGGGC 
AGTGGTGTGC CCCCACGTCC TGAGGAAGCA GATGAGACCT GGGACTCAAA GGAAGACAAA 
ATTCACAATG CTGAGAACAT CCAGCCCGGG GAACAGAAGT ATGAATATAA GTCAGATCAG 
TGGAAGCCTC TAAACCTAGA GGAGAAAAAA CGTTACGACC GTGAGTTCCT GCTTGGTTTT 
CAGTTCATCT TTGCCAGTAT GCAGAAGCCA GAGGGATTGC CACATATCAG TGACGTGGTG 
CTGGACAAGG CCAATAAAAC ACCACTGCGG CCACTGGATC CCACTAGACT ACAAGGCATA 
AATTGTGGCC CAGACTTCAC TCCATCCTTT GCCAACCTTG GCCGGACAAC CCTTAGCACC 
CGTGGGCCCC CAAGGGGTGG GCCAGGTGGG GAGCTGCCCC GTGGGCCGGC TGGCCTGGGA 
CCCCGGCGCT CTCAGCAGGG ACCCCGAAAA GAACCACGCA AGATCATTGC CACAGTGTTA 
ATGACCGAAG ATATAAAACT GAACAAAGCA GAGAAAGCCT GGAAACCCAG CAGCAAGCGG 
ACGGCGGCTG ATAAGGATCG AGGGGAAGAA GATGCTGATG GCAGCAAAAC CCAGGACCTA 
TTCCGCAGGG TGCGCTCCAT CCTGAATAAA CTGACACCCC AGATGTTCCA GCAGCTGATG 
AAGCAAGTGA CGCAGCTGGC CATCGACACC GAGGAACGCC TCAAAGGGGT CATTGACCTC 
ATTTTTGAGA AGGCCATTTC AGAGCCCAAC TTCTCTGTGG CCTATGCCAA CATGTGCCGC 
TGCCTCATGG CGCTGAAAGT GCCCACTACG GAAAAGCCAA CAGTGACTGT GAACTTCCGA 
AAGCTGTTGT TGAATCGATG TCAGAAGGAG TTTGAGAAAG ACAAAGATGA TGATGAGGTT 
TTTGAGAAGA AGCAAAAAGA GATGGATGAA GCTGCTACGG CAGAGGAACG AGGACGCCTG 
AAGGAAGAGC TGGAAGAGGC TCGGGACATA GCCCGGCGGC GCTCTTTAGG GAATATCAAG 
TTTATTGGAG AGTTGTTCAA ACTGAAGATG TTAACAGAGG CAATAATGCA TGACTGTGTG 
GTCAAACTGC TTAAGAACCA TGATGAAGAG TCCCTTGAGT GCCTTTGTCG TCTGCTCACC 
ACCATTGGCA AAGACCTGGA CTTTGAAAAA GCCAAGCCCC GAATGGATCA GTATTTCAAC 
CAGATGGAAA AAATCATTAA AGAAAAGAAG ACGTCATCCC GCATCCGCTT TATGCTGCAG 
GACGTGCTGG ATCTGCGAGG GAGCAATTGG GTGCCACGCC GAGGGGATCA GGGTCCCAAG 
ACCATTGACC AGATCCATAA GGAGGCTGAG ATGGAAGAAC ATCGAGAGCA CATCAAAGTG 
CAGCAGCTCA TGGCCAAGGG CAGTGACAAG CGTCGGGGCG GTCCTCCAGG CCCTCCCATC 
AGCCGTGGAC TTCCCCTTGT GGATGATGGT GGCTGGAACA CAGTTCCCAT CAGCAAAGGT 
AGCCGCCCCA TTGACACCTC ACGACTCACC AAGATCACCA AGCCTGGCTC CATCGATTCT 
AACAACCAGC TCTTTGCACC TGGAGGGCGA CTGAGCTGGG GCAAGGGCAG CAGCGGAGGC 
TCAGGAGCCA AGCCCTCAGA CGCAGCATCA GAAGCTGCTC GCCCAGCTAC TAGTACTTTG 
AATCGCTTCT CAGCCCTTCA ACAAGCGGTA CCCACAGAAA GCACAGATAA TAGACGTGTG 
GTGCAGAGGA GTAGCTTGAG CCGAGAACGA GGCGAGAAAG CTGGAGACCG AGGAGACCGC 
CTAGAGCGGA GTGAACGGGG AGGGGACCGT GGGGACCGGC TTGATCGTGC GCGGACACCT 
GCTACCAAGC GGAGCTTCAG CAAGGAAGTG GAGGAGCGGA GTAGAGAACG GCCCTCCCAG 
CCTGAGGGGC TGCGCAAGGC AGCTAGCCTC ACGGAGGATC GGGACCGTGG GCGGGATGCC 
GTGAAGCGAG AAGCTGCCCT ACCCCCAGTG AGCCCCCTGA AGGCGGCTCT CTCTGAGGAG 
GAGTTAGAGA AGAAATCCAA GGCTATCATT GAGGAATATC TCCATCTCAA TGACATGAAA 
GAGGCAGTCC AGTGCGTGCA GGAGCTGGCC TCACCCTCCT TGCTCTTCAT CTTTGTACGG 
CATGGTGTCG AGTCTACGCT GGAGCGCAGT GCCATTGCTC GTGAGCATAT GGGGCAGCTG 
CTGCACCAGC TGCTCTGTGC TGGGCATCTG TCTACTGCTC AGTACTACCA AGGGTTGTAT 
GAAATCTTGG AATTGGCTGA GGACATGGAA ATTGACATCC CCCACGTGTG GCTCTACCTA 
GCGGAACTGG TAACACCCAT TCTGCAGGAA GGTGGGGTGC CCATGGGGGA GCTGTTCAGG 
GAGATTACAA AGCCTCTGAG ACCGTTGGGC AAAGCTGCTT CCCTGTTGCT GGAGATCCTG 
GGCCTCCTGT GCAAAAGCAT GGGTCCTAAA AAGGTGGGGA CGCTGTGGCG AGAAGCCGGG 
CTTAGCTGGA AGGAATTTCT ACCTGAAGGC CAGGACATTG GTGCATTCGT CGCTGAACAG 
AAGGTGGAGT ATACCCTGGG AGAGGAGTCG GAAGCCCCTG GCCAGAGGGC ACTCCCCTCC 
GAGGAGCTGA ACAGGCAGCT GGAGAAGCTG CTGAAGGAGG GCAGCAGTAA CCAGCGGGTG 
TTCGACTGGA TAGAGGCCAA CCTGAGTGAG CAGCAGATAG TATCCAACAC GTTAGTTCGA 
GCCCTCATGA CGGCTGTCTG CTATTCTGCA ATTATTTTTG AGACTCCCCT CCGAGTGGAC 
GTTGCAGTGC TGAAAGCGCG AGCGAAGCTG CTGCAGAAAT ACCTGTGTGA CGAGCAGAAG 
GAGCTACAGG CGCTCTACGC CCTCCAGGCC CTTGTAGTGA CCTTAGAACA GCCTCCCAAC 
CTGCTGCGGA TGTTCTTTGA CGCACTGTAT GACGAGGACG TGGTGAAGGA GGATGCCTTC 
TACAGTTGGG AGAGTAGCAA GGACCCCGCT GAGCAGCAGG GCAAGGGTGT GGCCCTTAAA 
TCTGTCACAG CCTTCTTCAA GTGGCTCCGT GAAGCAGAGG AGGAGTCTGA CCACAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001278086.2
CDS	341..5020
Translation	MNTPSQPRQHFYPSRAQPPSSAASRVQSAAPARPGPAAHVYPAGSQVMMIPSQISYPASQGAYYIPGQGRSTYVVPTQQYPVQPGAPGFYPGASPTEFGTYAGAYYPAQGVQQFPTGVAPTPVLMNQPPQIAPKRERKTIRIRDPNQGGKDITEEIMSGARTASTPTPPQTGGGLEPQANGETPQVAVIVRPDDRSQGAIIADRPGLPGPEHSPSESQPSSPSPTPSPSPVLEPGSEPNLAVLSIPGDTMTTIQMSVEESTPISRETGEPYRLSPEPTPLAEPILEVEVTLSKPVPESEFSSSPLQAPTPLASHTVEIHEPNGMVPSEDLEPEVESSPELAPPPACPSESPVPIAPTAQPEELLNGAPSPPAVDLSPVSEPEEQAKEVTASMAPPTIPSATPATAPSATSPAQEEEMEEEEEEEEGEAGEAGEAESEKGGEELLPPESTPIPANLSQNLEAAAATQVAVSVPKRRRKIKELNKKEAVGDLLDAFKEANPAVPEVENQPPAGSNPGPESEGSGVPPRPEEADETWDSKEDKIHNAENIQPGEQKYEYKSDQWKPLNLEEKKRYDREFLLGFQFIFASMQKPEGLPHISDVVLDKANKTPLRPLDPTRLQGINCGPDFTPSFANLGRTTLSTRGPPRGGPGGELPRGPAGLGPRRSQQGPRKEPRKIIATVLMTEDIKLNKAEKAWKPSSKRTAADKDRGEEDADGSKTQDLFRRVRSILNKLTPQMFQQLMKQVTQLAIDTEERLKGVIDLIFEKAISEPNFSVAYANMCRCLMALKVPTTEKPTVTVNFRKLLLNRCQKEFEKDKDDDEVFEKKQKEMDEAATAEERGRLKEELEEARDIARRRSLGNIKFIGELFKLKMLTEAIMHDCVVKLLKNHDEESLECLCRLLTTIGKDLDFEKAKPRMDQYFNQMEKIIKEKKTSSRIRFMLQDVLDLRGSNWVPRRGDQGPKTIDQIHKEAEMEEHREHIKVQQLMAKGSDKRRGGPPGPPISRGLPLVDDGGWNTVPISKGSRPIDTSRLTKITKPGSIDSNNQLFAPGGRLSWGKGSSGGSGAKPSDAASEAARPATSTLNRFSALQQAVPTESTDNRRVVQRSSLSRERGEKAGDRGDRLERSERGGDRGDRLDRARTPATKRSFSKEVEERSRERPSQPEGLRKAASLTEDRDRGRDAVKREAALPPVSPLKAALSEEELEKKSKAIIEEYLHLNDMKEAVQCVQELASPSLLFIFVRHGVESTLERSAIAREHMGQLLHQLLCAGHLSTAQYYQGLYEILELAEDMEIDIPHVWLYLAELVTPILQEGGVPMGELFREITKPLRPLGKAASLLLEILGLLCKSMGPKKVGTLWREAGLSWKEFLPEGQDIGAFVAEQKVEYTLGEESEAPGQRALPSEELNRQLEKLLKEGSSNQRVFDWIEANLSEQQIVSNTLVRALMTAVCYSAIIFETPLRVDVAVLKARAKLLQKYLCDEQKELQALYALQALVVTLEQPPNLLRMFFDALYDEDVVKEDAFYSWESSKDPAEQQGKGVALKSVTAFFKWLREAEEESDHN

Target ORF information:

RefSeq Version	NM_001291157.2
Organism	Homo sapiens(human)
Definition	Homo sapiens eukaryotic translation initiation factor 4 gamma 1 (EIF4G1), transcript variant 8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001291157.2

ORF Insert Sequence:

ATGAACACGC CTTCTCAGCC CCGCCAGCAC TTCTACCCTA GCCGGGCCCA GCCCCCGAGC 
AGTGCAGCCT CCCGAGTGCA GAGTGCAGCC CCTGCCCGCC CTGGCCCAGC TGCCCATGTC 
TACCCTGCTG GATCCCAAGT AATGATGATC CCTTCCCAGA TCTCCTACCC AGCCTCCCAG 
GGGGCCTACT ACATCCCTGG ACAGGGGCGT TCCACATACG TTGTCCCGAC ACAGCAGTAC 
CCTGTGCAGC CAGGAGCCCC AGGCTTCTAT CCAGGTGCAA GCCCTACAGA ATTTGGGACC 
TACGCTGGCG CCTACTATCC AGCCCAAGGG GTGCAGCAGT TTCCCACTGG CGTGGCCCCC 
ACCCCAGTTT TGATGAACCA GCCACCCCAG ATTGCTCCCA AGAGGGAGCG TAAGACGATC 
CGAATTCGAG ATCCAAACCA AGGAGGAAAG GATATCACAG AGGAGATCAT GTCTGGGGCC 
CGCACTGCCT CCACACCCAC CCCTCCCCAG ACGGGAGGCG GTCTGGAGCC TCAAGCTAAT 
GGGGAGACGC CCCAGGTTGC TGTCATTGTC CGGCCAGATG ACCGGTCACA GGGAGCAATC 
ATTGCTGACC GGCCAGGGCT GCCTGGCCCA GAGCATAGCC CTTCAGAATC CCAGCCTTCG 
TCGCCTTCTC CGACCCCATC ACCATCCCCA GTCTTGGAAC CGGGGTCTGA GCCTAATCTC 
GCAGTCCTCT CTATTCCTGG GGACACTATG ACAACTATAC AAATGTCTGT AGAAGAATCA 
ACCCCCATCT CCCGTGAAAC TGGGGAGCCA TATCGCCTCT CTCCAGAACC CACTCCTCTC 
GCCGAACCCA TACTGGAAGT AGAAGTGACA CTTAGCAAAC CGGTTCCAGA ATCTGAGTTT 
TCTTCCAGTC CTCTCCAGGC TCCCACCCCT TTGGCATCTC ACACAGTGGA AATTCATGAG 
CCTAATGGCA TGGTCCCATC TGAAGATCTG GAACCAGAGG TGGAGTCAAG CCCAGAGCTT 
GCTCCTCCCC CAGCTTGCCC CTCCGAATCC CCTGTGCCCA TTGCTCCAAC TGCCCAACCT 
GAGGAACTGC TCAACGGAGC CCCCTCGCCA CCAGCTGTGG ACTTAAGCCC AGTCAGTGAG 
CCAGAGGAGC AGGCCAAGGA GGTGACAGCA TCAATGGCGC CCCCCACCAT CCCCTCTGCT 
ACTCCAGCTA CGGCTCCTTC AGCTACTTCC CCAGCTCAGG AGGAGGAAAT GGAAGAAGAA 
GAAGAAGAGG AAGAAGGAGA AGCAGGAGAA GCAGGAGAAG CTGAGAGTGA GAAAGGAGGA 
GAGGAACTGC TCCCCCCAGA GAGTACCCCT ATTCCAGCCA ACTTGTCTCA GAATTTGGAG 
GCAGCAGCAG CCACTCAAGT GGCAGTATCT GTGCCAAAGA GGAGACGGAA AATTAAGGAG 
CTAAATAAGA AGGAGGCTGT TGGAGACCTT CTGGATGCCT TCAAGGAGGC GAACCCGGCA 
GTACCAGAGG TGGAAAATCA GCCTCCTGCA GGCAGCAATC CAGGCCCAGA GTCTGAGGGC 
AGTGGTGTGC CCCCACGTCC TGAGGAAGCA GATGAGACCT GGGACTCAAA GGAAGACAAA 
ATTCACAATG CTGAGAACAT CCAGCCCGGG GAACAGAAGT ATGAATATAA GTCAGATCAG 
TGGAAGCCTC TAAACCTAGA GGAGAAAAAA CGTTACGACC GTGAGTTCCT GCTTGGTTTT 
CAGTTCATCT TTGCCAGTAT GCAGAAGCCA GAGGGATTGC CACATATCAG TGACGTGGTG 
CTGGACAAGG CCAATAAAAC ACCACTGCGG CCACTGGATC CCACTAGACT ACAAGGCATA 
AATTGTGGCC CAGACTTCAC TCCATCCTTT GCCAACCTTG GCCGGACAAC CCTTAGCACC 
CGTGGGCCCC CAAGGGGTGG GCCAGGTGGG GAGCTGCCCC GTGGGCCGGC TGGCCTGGGA 
CCCCGGCGCT CTCAGCAGGG ACCCCGAAAA GAACCACGCA AGATCATTGC CACAGTGTTA 
ATGACCGAAG ATATAAAACT GAACAAAGCA GAGAAAGCCT GGAAACCCAG CAGCAAGCGG 
ACGGCGGCTG ATAAGGATCG AGGGGAAGAA GATGCTGATG GCAGCAAAAC CCAGGACCTA 
TTCCGCAGGG TGCGCTCCAT CCTGAATAAA CTGACACCCC AGATGTTCCA GCAGCTGATG 
AAGCAAGTGA CGCAGCTGGC CATCGACACC GAGGAACGCC TCAAAGGGGT CATTGACCTC 
ATTTTTGAGA AGGCCATTTC AGAGCCCAAC TTCTCTGTGG CCTATGCCAA CATGTGCCGC 
TGCCTCATGG CGCTGAAAGT GCCCACTACG GAAAAGCCAA CAGTGACTGT GAACTTCCGA 
AAGCTGTTGT TGAATCGATG TCAGAAGGAG TTTGAGAAAG ACAAAGATGA TGATGAGGTT 
TTTGAGAAGA AGCAAAAAGA GATGGATGAA GCTGCTACGG CAGAGGAACG AGGACGCCTG 
AAGGAAGAGC TGGAAGAGGC TCGGGACATA GCCCGGCGGC GCTCTTTAGG GAATATCAAG 
TTTATTGGAG AGTTGTTCAA ACTGAAGATG TTAACAGAGG CAATAATGCA TGACTGTGTG 
GTCAAACTGC TTAAGAACCA TGATGAAGAG TCCCTTGAGT GCCTTTGTCG TCTGCTCACC 
ACCATTGGCA AAGACCTGGA CTTTGAAAAA GCCAAGCCCC GAATGGATCA GTATTTCAAC 
CAGATGGAAA AAATCATTAA AGAAAAGAAG ACGTCATCCC GCATCCGCTT TATGCTGCAG 
GACGTGCTGG ATCTGCGAGG GAGCAATTGG GTGCCACGCC GAGGGGATCA GGGTCCCAAG 
ACCATTGACC AGATCCATAA GGAGGCTGAG ATGGAAGAAC ATCGAGAGCA CATCAAAGTG 
CAGCAGCTCA TGGCCAAGGG CAGTGACAAG CGTCGGGGCG GTCCTCCAGG CCCTCCCATC 
AGCCGTGGAC TTCCCCTTGT GGATGATGGT GGCTGGAACA CAGTTCCCAT CAGCAAAGGT 
AGCCGCCCCA TTGACACCTC ACGACTCACC AAGATCACCA AGCCTGGCTC CATCGATTCT 
AACAACCAGC TCTTTGCACC TGGAGGGCGA CTGAGCTGGG GCAAGGGCAG CAGCGGAGGC 
TCAGGAGCCA AGCCCTCAGA CGCAGCATCA GAAGCTGCTC GCCCAGCTAC TAGTACTTTG 
AATCGCTTCT CAGCCCTTCA ACAAGCGGTA CCCACAGAAA GCACAGATAA TAGACGTGTG 
GTGCAGAGGA GTAGCTTGAG CCGAGAACGA GGCGAGAAAG CTGGAGACCG AGGAGACCGC 
CTAGAGCGGA GTGAACGGGG AGGGGACCGT GGGGACCGGC TTGATCGTGC GCGGACACCT 
GCTACCAAGC GGAGCTTCAG CAAGGAAGTG GAGGAGCGGA GTAGAGAACG GCCCTCCCAG 
CCTGAGGGGC TGCGCAAGGC AGCTAGCCTC ACGGAGGATC GGGACCGTGG GCGGGATGCC 
GTGAAGCGAG AAGCTGCCCT ACCCCCAGTG AGCCCCCTGA AGGCGGCTCT CTCTGAGGAG 
GAGTTAGAGA AGAAATCCAA GGCTATCATT GAGGAATATC TCCATCTCAA TGACATGAAA 
GAGGCAGTCC AGTGCGTGCA GGAGCTGGCC TCACCCTCCT TGCTCTTCAT CTTTGTACGG 
CATGGTGTCG AGTCTACGCT GGAGCGCAGT GCCATTGCTC GTGAGCATAT GGGGCAGCTG 
CTGCACCAGC TGCTCTGTGC TGGGCATCTG TCTACTGCTC AGTACTACCA AGGGTTGTAT 
GAAATCTTGG AATTGGCTGA GGACATGGAA ATTGACATCC CCCACGTGTG GCTCTACCTA 
GCGGAACTGG TAACACCCAT TCTGCAGGAA GGTGGGGTGC CCATGGGGGA GCTGTTCAGG 
GAGATTACAA AGCCTCTGAG ACCGTTGGGC AAAGCTGCTT CCCTGTTGCT GGAGATCCTG 
GGCCTCCTGT GCAAAAGCAT GGGTCCTAAA AAGGTGGGGA CGCTGTGGCG AGAAGCCGGG 
CTTAGCTGGA AGGAATTTCT ACCTGAAGGC CAGGACATTG GTGCATTCGT CGCTGAACAG 
AAGGTGGAGT ATACCCTGGG AGAGGAGTCG GAAGCCCCTG GCCAGAGGGC ACTCCCCTCC 
GAGGAGCTGA ACAGGCAGCT GGAGAAGCTG CTGAAGGAGG GCAGCAGTAA CCAGCGGGTG 
TTCGACTGGA TAGAGGCCAA CCTGAGTGAG CAGCAGATAG TATCCAACAC GTTAGTTCGA 
GCCCTCATGA CGGCTGTCTG CTATTCTGCA ATTATTTTTG AGACTCCCCT CCGAGTGGAC 
GTTGCAGTGC TGAAAGCGCG AGCGAAGCTG CTGCAGAAAT ACCTGTGTGA CGAGCAGAAG 
GAGCTACAGG CGCTCTACGC CCTCCAGGCC CTTGTAGTGA CCTTAGAACA GCCTCCCAAC 
CTGCTGCGGA TGTTCTTTGA CGCACTGTAT GACGAGGACG TGGTGAAGGA GGATGCCTTC 
TACAGTTGGG AGAGTAGCAA GGACCCCGCT GAGCAGCAGG GCAAGGGTGT GGCCCTTAAA 
TCTGTCACAG CCTTCTTCAA GTGGCTCCGT GAAGCAGAGG AGGAGTCTGA CCACAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EIF4G1 ( NM_198244.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_937887.2 Homo sapiens eukaryotic translation initiation factor 4 gamma 1 (EIF4G1), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu108656
Clone ID Related Accession (Same CDS sequence)	NM_198244.3
Accession Version	NM_198244.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4539bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-29
Organism	Homo sapiens(human)
Product	eukaryotic translation initiation factor 4 gamma 1 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC078797.20. On Jun 2, 2019 this sequence version replaced NM_198244.2. Transcript Variant: This variant (3) differs in the 5' UTR, lacks a portion of the 5' coding region, uses a downstream start codon, and uses an alternate in-frame splice site in the central coding region, compared to variant 1. The resulting isoform (2) is shorter at the N-terminus and lacks an internal residue, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK299763.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGATGATCC CTTCCCAGAT CTCCTACCCA GCCTCCCAGG GGGCCTACTA CATCCCTGGA 
CAGGGGCGTT CCACATACGT TGTCCCGACA CAGCAGTACC CTGTGCAGCC AGGAGCCCCA 
GGCTTCTATC CAGGTGCAAG CCCTACAGAA TTTGGGACCT ACGCTGGCGC CTACTATCCA 
GCCCAAGGGG TGCAGCAGTT TCCCACTGGC GTGGCCCCCA CCCCAGTTTT GATGAACCAG 
CCACCCCAGA TTGCTCCCAA GAGGGAGCGT AAGACGATCC GAATTCGAGA TCCAAACCAA 
GGAGGAAAGG ATATCACAGA GGAGATCATG TCTGGGGCCC GCACTGCCTC CACACCCACC 
CCTCCCCAGA CGGGAGGCGG TCTGGAGCCT CAAGCTAATG GGGAGACGCC CCAGGTTGCT 
GTCATTGTCC GGCCAGATGA CCGGTCACAG GGAGCAATCA TTGCTGACCG GCCAGGGCTG 
CCTGGCCCAG AGCATAGCCC TTCAGAATCC CAGCCTTCGT CGCCTTCTCC GACCCCATCA 
CCATCCCCAG TCTTGGAACC GGGGTCTGAG CCTAATCTCG CAGTCCTCTC TATTCCTGGG 
GACACTATGA CAACTATACA AATGTCTGTA GAAGAATCAA CCCCCATCTC CCGTGAAACT 
GGGGAGCCAT ATCGCCTCTC TCCAGAACCC ACTCCTCTCG CCGAACCCAT ACTGGAAGTA 
GAAGTGACAC TTAGCAAACC GGTTCCAGAA TCTGAGTTTT CTTCCAGTCC TCTCCAGGCT 
CCCACCCCTT TGGCATCTCA CACAGTGGAA ATTCATGAGC CTAATGGCAT GGTCCCATCT 
GAAGATCTGG AACCAGAGGT GGAGTCAAGC CCAGAGCTTG CTCCTCCCCC AGCTTGCCCC 
TCCGAATCCC CTGTGCCCAT TGCTCCAACT GCCCAACCTG AGGAACTGCT CAACGGAGCC 
CCCTCGCCAC CAGCTGTGGA CTTAAGCCCA GTCAGTGAGC CAGAGGAGCA GGCCAAGGAG 
GTGACAGCAT CAATGGCGCC CCCCACCATC CCCTCTGCTA CTCCAGCTAC GGCTCCTTCA 
GCTACTTCCC CAGCTCAGGA GGAGGAAATG GAAGAAGAAG AAGAAGAGGA AGAAGGAGAA 
GCAGGAGAAG CAGGAGAAGC TGAGAGTGAG AAAGGAGGAG AGGAACTGCT CCCCCCAGAG 
AGTACCCCTA TTCCAGCCAA CTTGTCTCAG AATTTGGAGG CAGCAGCAGC CACTCAAGTG 
GCAGTATCTG TGCCAAAGAG GAGACGGAAA ATTAAGGAGC TAAATAAGAA GGAGGCTGTT 
GGAGACCTTC TGGATGCCTT CAAGGAGGCG AACCCGGCAG TACCAGAGGT GGAAAATCAG 
CCTCCTGCAG GCAGCAATCC AGGCCCAGAG TCTGAGGGCA GTGGTGTGCC CCCACGTCCT 
GAGGAAGCAG ATGAGACCTG GGACTCAAAG GAAGACAAAA TTCACAATGC TGAGAACATC 
CAGCCCGGGG AACAGAAGTA TGAATATAAG TCAGATCAGT GGAAGCCTCT AAACCTAGAG 
GAGAAAAAAC GTTACGACCG TGAGTTCCTG CTTGGTTTTC AGTTCATCTT TGCCAGTATG 
CAGAAGCCAG AGGGATTGCC ACATATCAGT GACGTGGTGC TGGACAAGGC CAATAAAACA 
CCACTGCGGC CACTGGATCC CACTAGACTA CAAGGCATAA ATTGTGGCCC AGACTTCACT 
CCATCCTTTG CCAACCTTGG CCGGACAACC CTTAGCACCC GTGGGCCCCC AAGGGGTGGG 
CCAGGTGGGG AGCTGCCCCG TGGGCCGGCT GGCCTGGGAC CCCGGCGCTC TCAGCAGGGA 
CCCCGAAAAG AACCACGCAA GATCATTGCC ACAGTGTTAA TGACCGAAGA TATAAAACTG 
AACAAAGCAG AGAAAGCCTG GAAACCCAGC AGCAAGCGGA CGGCGGCTGA TAAGGATCGA 
GGGGAAGAAG ATGCTGATGG CAGCAAAACC CAGGACCTAT TCCGCAGGGT GCGCTCCATC 
CTGAATAAAC TGACACCCCA GATGTTCCAG CAGCTGATGA AGCAAGTGAC GCAGCTGGCC 
ATCGACACCG AGGAACGCCT CAAAGGGGTC ATTGACCTCA TTTTTGAGAA GGCCATTTCA 
GAGCCCAACT TCTCTGTGGC CTATGCCAAC ATGTGCCGCT GCCTCATGGC GCTGAAAGTG 
CCCACTACGG AAAAGCCAAC AGTGACTGTG AACTTCCGAA AGCTGTTGTT GAATCGATGT 
CAGAAGGAGT TTGAGAAAGA CAAAGATGAT GATGAGGTTT TTGAGAAGAA GCAAAAAGAG 
ATGGATGAAG CTGCTACGGC AGAGGAACGA GGACGCCTGA AGGAAGAGCT GGAAGAGGCT 
CGGGACATAG CCCGGCGGCG CTCTTTAGGG AATATCAAGT TTATTGGAGA GTTGTTCAAA 
CTGAAGATGT TAACAGAGGC AATAATGCAT GACTGTGTGG TCAAACTGCT TAAGAACCAT 
GATGAAGAGT CCCTTGAGTG CCTTTGTCGT CTGCTCACCA CCATTGGCAA AGACCTGGAC 
TTTGAAAAAG CCAAGCCCCG AATGGATCAG TATTTCAACC AGATGGAAAA AATCATTAAA 
GAAAAGAAGA CGTCATCCCG CATCCGCTTT ATGCTGCAGG ACGTGCTGGA TCTGCGAGGG 
AGCAATTGGG TGCCACGCCG AGGGGATCAG GGTCCCAAGA CCATTGACCA GATCCATAAG 
GAGGCTGAGA TGGAAGAACA TCGAGAGCAC ATCAAAGTGC AGCAGCTCAT GGCCAAGGGC 
AGTGACAAGC GTCGGGGCGG TCCTCCAGGC CCTCCCATCA GCCGTGGACT TCCCCTTGTG 
GATGATGGTG GCTGGAACAC AGTTCCCATC AGCAAAGGTA GCCGCCCCAT TGACACCTCA 
CGACTCACCA AGATCACCAA GCCTGGCTCC ATCGATTCTA ACAACCAGCT CTTTGCACCT 
GGAGGGCGAC TGAGCTGGGG CAAGGGCAGC AGCGGAGGCT CAGGAGCCAA GCCCTCAGAC 
GCAGCATCAG AAGCTGCTCG CCCAGCTACT AGTACTTTGA ATCGCTTCTC AGCCCTTCAA 
CAAGCGGTAC CCACAGAAAG CACAGATAAT AGACGTGTGG TGCAGAGGAG TAGCTTGAGC 
CGAGAACGAG GCGAGAAAGC TGGAGACCGA GGAGACCGCC TAGAGCGGAG TGAACGGGGA 
GGGGACCGTG GGGACCGGCT TGATCGTGCG CGGACACCTG CTACCAAGCG GAGCTTCAGC 
AAGGAAGTGG AGGAGCGGAG TAGAGAACGG CCCTCCCAGC CTGAGGGGCT GCGCAAGGCA 
GCTAGCCTCA CGGAGGATCG GGACCGTGGG CGGGATGCCG TGAAGCGAGA AGCTGCCCTA 
CCCCCAGTGA GCCCCCTGAA GGCGGCTCTC TCTGAGGAGG AGTTAGAGAA GAAATCCAAG 
GCTATCATTG AGGAATATCT CCATCTCAAT GACATGAAAG AGGCAGTCCA GTGCGTGCAG 
GAGCTGGCCT CACCCTCCTT GCTCTTCATC TTTGTACGGC ATGGTGTCGA GTCTACGCTG 
GAGCGCAGTG CCATTGCTCG TGAGCATATG GGGCAGCTGC TGCACCAGCT GCTCTGTGCT 
GGGCATCTGT CTACTGCTCA GTACTACCAA GGGTTGTATG AAATCTTGGA ATTGGCTGAG 
GACATGGAAA TTGACATCCC CCACGTGTGG CTCTACCTAG CGGAACTGGT AACACCCATT 
CTGCAGGAAG GTGGGGTGCC CATGGGGGAG CTGTTCAGGG AGATTACAAA GCCTCTGAGA 
CCGTTGGGCA AAGCTGCTTC CCTGTTGCTG GAGATCCTGG GCCTCCTGTG CAAAAGCATG 
GGTCCTAAAA AGGTGGGGAC GCTGTGGCGA GAAGCCGGGC TTAGCTGGAA GGAATTTCTA 
CCTGAAGGCC AGGACATTGG TGCATTCGTC GCTGAACAGA AGGTGGAGTA TACCCTGGGA 
GAGGAGTCGG AAGCCCCTGG CCAGAGGGCA CTCCCCTCCG AGGAGCTGAA CAGGCAGCTG 
GAGAAGCTGC TGAAGGAGGG CAGCAGTAAC CAGCGGGTGT TCGACTGGAT AGAGGCCAAC 
CTGAGTGAGC AGCAGATAGT ATCCAACACG TTAGTTCGAG CCCTCATGAC GGCTGTCTGC 
TATTCTGCAA TTATTTTTGA GACTCCCCTC CGAGTGGACG TTGCAGTGCT GAAAGCGCGA 
GCGAAGCTGC TGCAGAAATA CCTGTGTGAC GAGCAGAAGG AGCTACAGGC GCTCTACGCC 
CTCCAGGCCC TTGTAGTGAC CTTAGAACAG CCTCCCAACC TGCTGCGGAT GTTCTTTGAC 
GCACTGTATG ACGAGGACGT GGTGAAGGAG GATGCCTTCT ACAGTTGGGA GAGTAGCAAG 
GACCCCGCTG AGCAGCAGGG CAAGGGTGTG GCCCTTAAAT CTGTCACAGC CTTCTTCAAG 
TGGCTCCGTG AAGCAGAGGA GGAGTCTGAC CACAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_937887.2
CDS	184..4722
Translation	MMIPSQISYPASQGAYYIPGQGRSTYVVPTQQYPVQPGAPGFYPGASPTEFGTYAGAYYPAQGVQQFPTGVAPTPVLMNQPPQIAPKRERKTIRIRDPNQGGKDITEEIMSGARTASTPTPPQTGGGLEPQANGETPQVAVIVRPDDRSQGAIIADRPGLPGPEHSPSESQPSSPSPTPSPSPVLEPGSEPNLAVLSIPGDTMTTIQMSVEESTPISRETGEPYRLSPEPTPLAEPILEVEVTLSKPVPESEFSSSPLQAPTPLASHTVEIHEPNGMVPSEDLEPEVESSPELAPPPACPSESPVPIAPTAQPEELLNGAPSPPAVDLSPVSEPEEQAKEVTASMAPPTIPSATPATAPSATSPAQEEEMEEEEEEEEGEAGEAGEAESEKGGEELLPPESTPIPANLSQNLEAAAATQVAVSVPKRRRKIKELNKKEAVGDLLDAFKEANPAVPEVENQPPAGSNPGPESEGSGVPPRPEEADETWDSKEDKIHNAENIQPGEQKYEYKSDQWKPLNLEEKKRYDREFLLGFQFIFASMQKPEGLPHISDVVLDKANKTPLRPLDPTRLQGINCGPDFTPSFANLGRTTLSTRGPPRGGPGGELPRGPAGLGPRRSQQGPRKEPRKIIATVLMTEDIKLNKAEKAWKPSSKRTAADKDRGEEDADGSKTQDLFRRVRSILNKLTPQMFQQLMKQVTQLAIDTEERLKGVIDLIFEKAISEPNFSVAYANMCRCLMALKVPTTEKPTVTVNFRKLLLNRCQKEFEKDKDDDEVFEKKQKEMDEAATAEERGRLKEELEEARDIARRRSLGNIKFIGELFKLKMLTEAIMHDCVVKLLKNHDEESLECLCRLLTTIGKDLDFEKAKPRMDQYFNQMEKIIKEKKTSSRIRFMLQDVLDLRGSNWVPRRGDQGPKTIDQIHKEAEMEEHREHIKVQQLMAKGSDKRRGGPPGPPISRGLPLVDDGGWNTVPISKGSRPIDTSRLTKITKPGSIDSNNQLFAPGGRLSWGKGSSGGSGAKPSDAASEAARPATSTLNRFSALQQAVPTESTDNRRVVQRSSLSRERGEKAGDRGDRLERSERGGDRGDRLDRARTPATKRSFSKEVEERSRERPSQPEGLRKAASLTEDRDRGRDAVKREAALPPVSPLKAALSEEELEKKSKAIIEEYLHLNDMKEAVQCVQELASPSLLFIFVRHGVESTLERSAIAREHMGQLLHQLLCAGHLSTAQYYQGLYEILELAEDMEIDIPHVWLYLAELVTPILQEGGVPMGELFREITKPLRPLGKAASLLLEILGLLCKSMGPKKVGTLWREAGLSWKEFLPEGQDIGAFVAEQKVEYTLGEESEAPGQRALPSEELNRQLEKLLKEGSSNQRVFDWIEANLSEQQIVSNTLVRALMTAVCYSAIIFETPLRVDVAVLKARAKLLQKYLCDEQKELQALYALQALVVTLEQPPNLLRMFFDALYDEDVVKEDAFYSWESSKDPAEQQGKGVALKSVTAFFKWLREAEEESDHN

Target ORF information:

RefSeq Version	NM_198244.3
Organism	Homo sapiens(human)
Definition	Homo sapiens eukaryotic translation initiation factor 4 gamma 1 (EIF4G1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_198244.3

ORF Insert Sequence:

ATGATGATCC CTTCCCAGAT CTCCTACCCA GCCTCCCAGG GGGCCTACTA CATCCCTGGA 
CAGGGGCGTT CCACATACGT TGTCCCGACA CAGCAGTACC CTGTGCAGCC AGGAGCCCCA 
GGCTTCTATC CAGGTGCAAG CCCTACAGAA TTTGGGACCT ACGCTGGCGC CTACTATCCA 
GCCCAAGGGG TGCAGCAGTT TCCCACTGGC GTGGCCCCCA CCCCAGTTTT GATGAACCAG 
CCACCCCAGA TTGCTCCCAA GAGGGAGCGT AAGACGATCC GAATTCGAGA TCCAAACCAA 
GGAGGAAAGG ATATCACAGA GGAGATCATG TCTGGGGCCC GCACTGCCTC CACACCCACC 
CCTCCCCAGA CGGGAGGCGG TCTGGAGCCT CAAGCTAATG GGGAGACGCC CCAGGTTGCT 
GTCATTGTCC GGCCAGATGA CCGGTCACAG GGAGCAATCA TTGCTGACCG GCCAGGGCTG 
CCTGGCCCAG AGCATAGCCC TTCAGAATCC CAGCCTTCGT CGCCTTCTCC GACCCCATCA 
CCATCCCCAG TCTTGGAACC GGGGTCTGAG CCTAATCTCG CAGTCCTCTC TATTCCTGGG 
GACACTATGA CAACTATACA AATGTCTGTA GAAGAATCAA CCCCCATCTC CCGTGAAACT 
GGGGAGCCAT ATCGCCTCTC TCCAGAACCC ACTCCTCTCG CCGAACCCAT ACTGGAAGTA 
GAAGTGACAC TTAGCAAACC GGTTCCAGAA TCTGAGTTTT CTTCCAGTCC TCTCCAGGCT 
CCCACCCCTT TGGCATCTCA CACAGTGGAA ATTCATGAGC CTAATGGCAT GGTCCCATCT 
GAAGATCTGG AACCAGAGGT GGAGTCAAGC CCAGAGCTTG CTCCTCCCCC AGCTTGCCCC 
TCCGAATCCC CTGTGCCCAT TGCTCCAACT GCCCAACCTG AGGAACTGCT CAACGGAGCC 
CCCTCGCCAC CAGCTGTGGA CTTAAGCCCA GTCAGTGAGC CAGAGGAGCA GGCCAAGGAG 
GTGACAGCAT CAATGGCGCC CCCCACCATC CCCTCTGCTA CTCCAGCTAC GGCTCCTTCA 
GCTACTTCCC CAGCTCAGGA GGAGGAAATG GAAGAAGAAG AAGAAGAGGA AGAAGGAGAA 
GCAGGAGAAG CAGGAGAAGC TGAGAGTGAG AAAGGAGGAG AGGAACTGCT CCCCCCAGAG 
AGTACCCCTA TTCCAGCCAA CTTGTCTCAG AATTTGGAGG CAGCAGCAGC CACTCAAGTG 
GCAGTATCTG TGCCAAAGAG GAGACGGAAA ATTAAGGAGC TAAATAAGAA GGAGGCTGTT 
GGAGACCTTC TGGATGCCTT CAAGGAGGCG AACCCGGCAG TACCAGAGGT GGAAAATCAG 
CCTCCTGCAG GCAGCAATCC AGGCCCAGAG TCTGAGGGCA GTGGTGTGCC CCCACGTCCT 
GAGGAAGCAG ATGAGACCTG GGACTCAAAG GAAGACAAAA TTCACAATGC TGAGAACATC 
CAGCCCGGGG AACAGAAGTA TGAATATAAG TCAGATCAGT GGAAGCCTCT AAACCTAGAG 
GAGAAAAAAC GTTACGACCG TGAGTTCCTG CTTGGTTTTC AGTTCATCTT TGCCAGTATG 
CAGAAGCCAG AGGGATTGCC ACATATCAGT GACGTGGTGC TGGACAAGGC CAATAAAACA 
CCACTGCGGC CACTGGATCC CACTAGACTA CAAGGCATAA ATTGTGGCCC AGACTTCACT 
CCATCCTTTG CCAACCTTGG CCGGACAACC CTTAGCACCC GTGGGCCCCC AAGGGGTGGG 
CCAGGTGGGG AGCTGCCCCG TGGGCCGGCT GGCCTGGGAC CCCGGCGCTC TCAGCAGGGA 
CCCCGAAAAG AACCACGCAA GATCATTGCC ACAGTGTTAA TGACCGAAGA TATAAAACTG 
AACAAAGCAG AGAAAGCCTG GAAACCCAGC AGCAAGCGGA CGGCGGCTGA TAAGGATCGA 
GGGGAAGAAG ATGCTGATGG CAGCAAAACC CAGGACCTAT TCCGCAGGGT GCGCTCCATC 
CTGAATAAAC TGACACCCCA GATGTTCCAG CAGCTGATGA AGCAAGTGAC GCAGCTGGCC 
ATCGACACCG AGGAACGCCT CAAAGGGGTC ATTGACCTCA TTTTTGAGAA GGCCATTTCA 
GAGCCCAACT TCTCTGTGGC CTATGCCAAC ATGTGCCGCT GCCTCATGGC GCTGAAAGTG 
CCCACTACGG AAAAGCCAAC AGTGACTGTG AACTTCCGAA AGCTGTTGTT GAATCGATGT 
CAGAAGGAGT TTGAGAAAGA CAAAGATGAT GATGAGGTTT TTGAGAAGAA GCAAAAAGAG 
ATGGATGAAG CTGCTACGGC AGAGGAACGA GGACGCCTGA AGGAAGAGCT GGAAGAGGCT 
CGGGACATAG CCCGGCGGCG CTCTTTAGGG AATATCAAGT TTATTGGAGA GTTGTTCAAA 
CTGAAGATGT TAACAGAGGC AATAATGCAT GACTGTGTGG TCAAACTGCT TAAGAACCAT 
GATGAAGAGT CCCTTGAGTG CCTTTGTCGT CTGCTCACCA CCATTGGCAA AGACCTGGAC 
TTTGAAAAAG CCAAGCCCCG AATGGATCAG TATTTCAACC AGATGGAAAA AATCATTAAA 
GAAAAGAAGA CGTCATCCCG CATCCGCTTT ATGCTGCAGG ACGTGCTGGA TCTGCGAGGG 
AGCAATTGGG TGCCACGCCG AGGGGATCAG GGTCCCAAGA CCATTGACCA GATCCATAAG 
GAGGCTGAGA TGGAAGAACA TCGAGAGCAC ATCAAAGTGC AGCAGCTCAT GGCCAAGGGC 
AGTGACAAGC GTCGGGGCGG TCCTCCAGGC CCTCCCATCA GCCGTGGACT TCCCCTTGTG 
GATGATGGTG GCTGGAACAC AGTTCCCATC AGCAAAGGTA GCCGCCCCAT TGACACCTCA 
CGACTCACCA AGATCACCAA GCCTGGCTCC ATCGATTCTA ACAACCAGCT CTTTGCACCT 
GGAGGGCGAC TGAGCTGGGG CAAGGGCAGC AGCGGAGGCT CAGGAGCCAA GCCCTCAGAC 
GCAGCATCAG AAGCTGCTCG CCCAGCTACT AGTACTTTGA ATCGCTTCTC AGCCCTTCAA 
CAAGCGGTAC CCACAGAAAG CACAGATAAT AGACGTGTGG TGCAGAGGAG TAGCTTGAGC 
CGAGAACGAG GCGAGAAAGC TGGAGACCGA GGAGACCGCC TAGAGCGGAG TGAACGGGGA 
GGGGACCGTG GGGACCGGCT TGATCGTGCG CGGACACCTG CTACCAAGCG GAGCTTCAGC 
AAGGAAGTGG AGGAGCGGAG TAGAGAACGG CCCTCCCAGC CTGAGGGGCT GCGCAAGGCA 
GCTAGCCTCA CGGAGGATCG GGACCGTGGG CGGGATGCCG TGAAGCGAGA AGCTGCCCTA 
CCCCCAGTGA GCCCCCTGAA GGCGGCTCTC TCTGAGGAGG AGTTAGAGAA GAAATCCAAG 
GCTATCATTG AGGAATATCT CCATCTCAAT GACATGAAAG AGGCAGTCCA GTGCGTGCAG 
GAGCTGGCCT CACCCTCCTT GCTCTTCATC TTTGTACGGC ATGGTGTCGA GTCTACGCTG 
GAGCGCAGTG CCATTGCTCG TGAGCATATG GGGCAGCTGC TGCACCAGCT GCTCTGTGCT 
GGGCATCTGT CTACTGCTCA GTACTACCAA GGGTTGTATG AAATCTTGGA ATTGGCTGAG 
GACATGGAAA TTGACATCCC CCACGTGTGG CTCTACCTAG CGGAACTGGT AACACCCATT 
CTGCAGGAAG GTGGGGTGCC CATGGGGGAG CTGTTCAGGG AGATTACAAA GCCTCTGAGA 
CCGTTGGGCA AAGCTGCTTC CCTGTTGCTG GAGATCCTGG GCCTCCTGTG CAAAAGCATG 
GGTCCTAAAA AGGTGGGGAC GCTGTGGCGA GAAGCCGGGC TTAGCTGGAA GGAATTTCTA 
CCTGAAGGCC AGGACATTGG TGCATTCGTC GCTGAACAGA AGGTGGAGTA TACCCTGGGA 
GAGGAGTCGG AAGCCCCTGG CCAGAGGGCA CTCCCCTCCG AGGAGCTGAA CAGGCAGCTG 
GAGAAGCTGC TGAAGGAGGG CAGCAGTAAC CAGCGGGTGT TCGACTGGAT AGAGGCCAAC 
CTGAGTGAGC AGCAGATAGT ATCCAACACG TTAGTTCGAG CCCTCATGAC GGCTGTCTGC 
TATTCTGCAA TTATTTTTGA GACTCCCCTC CGAGTGGACG TTGCAGTGCT GAAAGCGCGA 
GCGAAGCTGC TGCAGAAATA CCTGTGTGAC GAGCAGAAGG AGCTACAGGC GCTCTACGCC 
CTCCAGGCCC TTGTAGTGAC CTTAGAACAG CCTCCCAACC TGCTGCGGAT GTTCTTTGAC 
GCACTGTATG ACGAGGACGT GGTGAAGGAG GATGCCTTCT ACAGTTGGGA GAGTAGCAAG 
GACCCCGCTG AGCAGCAGGG CAAGGGTGTG GCCCTTAAAT CTGTCACAGC CTTCTTCAAG 
TGGCTCCGTG AAGCAGAGGA GGAGTCTGAC CACAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EIF4G1 ( NM_198244.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_937887.1 Homo sapiens eukaryotic translation initiation factor 4 gamma 1 (EIF4G1), transcript variant 3, mRNA.

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CloneID	OHu13778
Clone ID Related Accession (Same CDS sequence)	NM_198244.2
Accession Version	NM_198244.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4539bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-03-03
Organism	Homo sapiens(human)
Product	eukaryotic translation initiation factor 4 gamma 1 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CR980540.1, AK299763.1, BC010688.1, BC007788.2 and BQ011010.1. On Aug 13, 2010 this sequence version replaced NM_198244.1. Transcript Variant: This variant (3) differs in the 5' UTR, lacks a portion of the 5' coding region, uses a downstream start codon, and uses an alternate in-frame splice site in the central coding region, compared to variant 1. The resulting isoform (2) is shorter at the N-terminus and lacks an internal residue, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK299763.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGATGATCC CTTCCCAGAT CTCCTACCCA GCCTCCCAGG GGGCCTACTA CATCCCTGGA 
CAGGGGCGTT CCACATACGT TGTCCCGACA CAGCAGTACC CTGTGCAGCC AGGAGCCCCA 
GGCTTCTATC CAGGTGCAAG CCCTACAGAA TTTGGGACCT ACGCTGGCGC CTACTATCCA 
GCCCAAGGGG TGCAGCAGTT TCCCACTGGC GTGGCCCCCG CCCCAGTTTT GATGAACCAG 
CCACCCCAGA TTGCTCCCAA GAGGGAGCGT AAGACGATCC GAATTCGAGA TCCAAACCAA 
GGAGGAAAGG ATATCACAGA GGAGATCATG TCTGGGGCCC GCACTGCCTC CACACCCACC 
CCTCCCCAGA CGGGAGGCGG TCTGGAGCCT CAAGCTAATG GGGAGACGCC CCAGGTTGCT 
GTCATTGTCC GGCCAGATGA CCGGTCACAG GGAGCAATCA TTGCTGACCG GCCAGGGCTG 
CCTGGCCCAG AGCATAGCCC TTCAGAATCC CAGCCTTCGT CGCCTTCTCC GACCCCATCA 
CCATCCCCAG TCTTGGAACC GGGGTCTGAG CCTAATCTCG CAGTCCTCTC TATTCCTGGG 
GACACTATGA CAACTATACA AATGTCTGTA GAAGAATCAA CCCCCATCTC CCGTGAAACT 
GGGGAGCCAT ATCGCCTCTC TCCAGAACCC ACTCCTCTCG CCGAACCCAT ACTGGAAGTA 
GAAGTGACAC TTAGCAAACC GGTTCCAGAA TCTGAGTTTT CTTCCAGTCC TCTCCAGGCT 
CCCACCCCTT TGGCATCTCA CACAGTGGAA ATTCATGAGC CTAATGGCAT GGTCCCATCT 
GAAGATCTGG AACCAGAGGT GGAGTCAAGC CCAGAGCTTG CTCCTCCCCC AGCTTGCCCC 
TCCGAATCCC CTGTGCCCAT TGCTCCAACT GCCCAACCTG AGGAACTGCT CAACGGAGCC 
CCCTCGCCAC CAGCTGTGGA CTTAAGCCCA GTCAGTGAGC CAGAGGAGCA GGCCAAGGAG 
GTGACAGCAT CAATGGCGCC CCCCACCATC CCCTCTGCTA CTCCAGCTAC GGCTCCTTCA 
GCTACTTCCC CAGCTCAGGA GGAGGAAATG GAAGAAGAAG AAGAAGAGGA AGAAGGAGAA 
GCAGGAGAAG CAGGAGAAGC TGAGAGTGAG AAAGGAGGAG AGGAACTGCT CCCCCCAGAG 
AGTACCCCTA TTCCAGCCAA CTTGTCTCAG AATTTGGAGG CAGCAGCAGC CACTCAAGTG 
GCAGTATCTG TGCCAAAGAG GAGACGGAAA ATTAAGGAGC TAAATAAGAA GGAGGCTGTT 
GGAGACCTTC TGGATGCCTT CAAGGAGGCG AACCCGGCAG TACCAGAGGT GGAAAATCAG 
CCTCCTGCAG GCAGCAATCC AGGCCCAGAG TCTGAGGGCA GTGGTGTGCC CCCACGTCCT 
GAGGAAGCAG ATGAGACCTG GGACTCAAAG GAAGACAAAA TTCACAATGC TGAGAACATC 
CAGCCCGGGG AACAGAAGTA TGAATATAAG TCAGATCAGT GGAAGCCTCT AAACCTAGAG 
GAGAAAAAAC GTTACGACCG TGAGTTCCTG CTTGGTTTTC AGTTCATCTT TGCCAGTATG 
CAGAAGCCAG AGGGATTGCC ACATATCAGT GACGTGGTGC TGGACAAGGC CAATAAAACA 
CCACTGCGGC CACTGGATCC CACTAGACTA CAAGGCATAA ATTGTGGCCC AGACTTCACT 
CCATCCTTTG CCAACCTTGG CCGGACAACC CTTAGCACCC GTGGGCCCCC AAGGGGTGGG 
CCAGGTGGGG AGCTGCCCCG TGGGCCGGCT GGCCTGGGAC CCCGGCGCTC TCAGCAGGGA 
CCCCGAAAAG AACCACGCAA GATCATTGCC ACAGTGTTAA TGACCGAAGA TATAAAACTG 
AACAAAGCAG AGAAAGCCTG GAAACCCAGC AGCAAGCGGA CGGCGGCTGA TAAGGATCGA 
GGGGAAGAAG ATGCTGATGG CAGCAAAACC CAGGACCTAT TCCGCAGGGT GCGCTCCATC 
CTGAATAAAC TGACACCCCA GATGTTCCAG CAGCTGATGA AGCAAGTGAC GCAGCTGGCC 
ATCGACACCG AGGAACGCCT CAAAGGGGTC ATTGACCTCA TTTTTGAGAA GGCCATTTCA 
GAGCCCAACT TCTCTGTGGC CTATGCCAAC ATGTGCCGCT GCCTCATGGC GCTGAAAGTG 
CCCACTACGG AAAAGCCAAC AGTGACTGTG AACTTCCGAA AGCTGTTGTT GAATCGATGT 
CAGAAGGAGT TTGAGAAAGA CAAAGATGAT GATGAGGTTT TTGAGAAGAA GCAAAAAGAG 
ATGGATGAAG CTGCTACGGC AGAGGAACGA GGACGCCTGA AGGAAGAGCT GGAAGAGGCT 
CGGGACATAG CCCGGCGGCG CTCTTTAGGG AATATCAAGT TTATTGGAGA GTTGTTCAAA 
CTGAAGATGT TAACAGAGGC AATAATGCAT GACTGTGTGG TCAAACTGCT TAAGAACCAT 
GATGAAGAGT CCCTTGAGTG CCTTTGTCGT CTGCTCACCA CCATTGGCAA AGACCTGGAC 
TTTGAAAAAG CCAAGCCCCG AATGGATCAG TATTTCAACC AGATGGAAAA AATCATTAAA 
GAAAAGAAGA CGTCATCCCG CATCCGCTTT ATGCTGCAGG ACGTGCTGGA TCTGCGAGGG 
AGCAATTGGG TGCCACGCCG AGGGGATCAG GGTCCCAAGA CCATTGACCA GATCCATAAG 
GAGGCTGAGA TGGAAGAACA TCGAGAGCAC ATCAAAGTGC AGCAGCTCAT GGCCAAGGGC 
AGTGACAAGC GTCGGGGCGG TCCTCCAGGC CCTCCCATCA GCCGTGGACT TCCCCTTGTG 
GATGATGGTG GCTGGAACAC AGTTCCCATC AGCAAAGGTA GCCGCCCCAT TGACACCTCA 
CGACTCACCA AGATCACCAA GCCTGGCTCC ATCGATTCTA ACAACCAGCT CTTTGCACCT 
GGAGGGCGAC TGAGCTGGGG CAAGGGCAGC AGCGGAGGCT CAGGAGCCAA GCCCTCAGAC 
GCAGCATCAG AAGCTGCTCG CCCAGCTACT AGTACTTTGA ATCGCTTCTC AGCCCTTCAA 
CAAGCGGTAC CCACAGAAAG CACAGATAAT AGACGTGTGG TGCAGAGGAG TAGCTTGAGC 
CGAGAACGAG GCGAGAAAGC TGGAGACCGA GGAGACCGCC TAGAGCGGAG TGAACGGGGA 
GGGGACCGTG GGGACCGGCT TGATCGTGCG CGGACACCTG CTACCAAGCG GAGCTTCAGC 
AAGGAAGTGG AGGAGCGGAG TAGAGAACGG CCCTCCCAGC CTGAGGGGCT GCGCAAGGCA 
GCTAGCCTCA CGGAGGATCG GGACCGTGGG CGGGATGCCG TGAAGCGAGA AGCTGCCCTA 
CCCCCAGTGA GCCCCCTGAA GGCGGCTCTC TCTGAGGAGG AGTTAGAGAA GAAATCCAAG 
GCTATCATTG AGGAATATCT CCATCTCAAT GACATGAAAG AGGCAGTCCA GTGCGTGCAG 
GAGCTGGCCT CACCCTCCTT GCTCTTCATC TTTGTACGGC ATGGTGTCGA GTCTACGCTG 
GAGCGCAGTG CCATTGCTCG TGAGCATATG GGGCAGCTGC TGCACCAGCT GCTCTGTGCT 
GGGCATCTGT CTACTGCTCA GTACTACCAA GGGTTGTATG AAATCTTGGA ATTGGCTGAG 
GACATGGAAA TTGACATCCC CCACGTGTGG CTCTACCTAG CGGAACTGGT AACACCCATT 
CTGCAGGAAG GTGGGGTGCC CATGGGGGAG CTGTTCAGGG AGATTACAAA GCCTCTGAGA 
CCGTTGGGCA AAGCTGCTTC CCTGTTGCTG GAGATCCTGG GCCTCCTGTG CAAAAGCATG 
GGTCCTAAAA AGGTGGGGAC GCTGTGGCGA GAAGCCGGGC TTAGCTGGAA GGAATTTCTA 
CCTGAAGGCC AGGACATTGG TGCATTCGTC GCTGAACAGA AGGTGGAGTA TACCCTGGGA 
GAGGAGTCGG AAGCCCCTGG CCAGAGGGCA CTCCCCTCCG AGGAGCTGAA CAGGCAGCTG 
GAGAAGCTGC TGAAGGAGGG CAGCAGTAAC CAGCGGGTGT TCGACTGGAT AGAGGCCAAC 
CTGAGTGAGC AGCAGATAGT ATCCAACACG TTAGTTCGAG CCCTCATGAC GGCTGTCTGC 
TATTCTGCAA TTATTTTTGA GACTCCCCTC CGAGTGGACG TTGCAGTGCT GAAAGCGCGA 
GCGAAGCTGC TGCAGAAATA CCTGTGTGAC GAGCAGAAGG AGCTACAGGC GCTCTACGCC 
CTCCAGGCCC TTGTAGTGAC CTTAGAACAG CCTCCCAACC TGCTGCGGAT GTTCTTTGAC 
GCACTGTATG ACGAGGACGT GGTGAAGGAG GATGCCTTCT ACAGTTGGGA GAGTAGCAAG 
GACCCCGCTG AGCAGCAGGG CAAGGGTGTG GCCCTTAAAT CTGTCACAGC CTTCTTCAAG 
TGGCTCCGTG AAGCAGAGGA GGAGTCTGAC CACAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_937887.1
CDS	295..4833
Translation	MMIPSQISYPASQGAYYIPGQGRSTYVVPTQQYPVQPGAPGFYPGASPTEFGTYAGAYYPAQGVQQFPTGVAPAPVLMNQPPQIAPKRERKTIRIRDPNQGGKDITEEIMSGARTASTPTPPQTGGGLEPQANGETPQVAVIVRPDDRSQGAIIADRPGLPGPEHSPSESQPSSPSPTPSPSPVLEPGSEPNLAVLSIPGDTMTTIQMSVEESTPISRETGEPYRLSPEPTPLAEPILEVEVTLSKPVPESEFSSSPLQAPTPLASHTVEIHEPNGMVPSEDLEPEVESSPELAPPPACPSESPVPIAPTAQPEELLNGAPSPPAVDLSPVSEPEEQAKEVTASMAPPTIPSATPATAPSATSPAQEEEMEEEEEEEEGEAGEAGEAESEKGGEELLPPESTPIPANLSQNLEAAAATQVAVSVPKRRRKIKELNKKEAVGDLLDAFKEANPAVPEVENQPPAGSNPGPESEGSGVPPRPEEADETWDSKEDKIHNAENIQPGEQKYEYKSDQWKPLNLEEKKRYDREFLLGFQFIFASMQKPEGLPHISDVVLDKANKTPLRPLDPTRLQGINCGPDFTPSFANLGRTTLSTRGPPRGGPGGELPRGPAGLGPRRSQQGPRKEPRKIIATVLMTEDIKLNKAEKAWKPSSKRTAADKDRGEEDADGSKTQDLFRRVRSILNKLTPQMFQQLMKQVTQLAIDTEERLKGVIDLIFEKAISEPNFSVAYANMCRCLMALKVPTTEKPTVTVNFRKLLLNRCQKEFEKDKDDDEVFEKKQKEMDEAATAEERGRLKEELEEARDIARRRSLGNIKFIGELFKLKMLTEAIMHDCVVKLLKNHDEESLECLCRLLTTIGKDLDFEKAKPRMDQYFNQMEKIIKEKKTSSRIRFMLQDVLDLRGSNWVPRRGDQGPKTIDQIHKEAEMEEHREHIKVQQLMAKGSDKRRGGPPGPPISRGLPLVDDGGWNTVPISKGSRPIDTSRLTKITKPGSIDSNNQLFAPGGRLSWGKGSSGGSGAKPSDAASEAARPATSTLNRFSALQQAVPTESTDNRRVVQRSSLSRERGEKAGDRGDRLERSERGGDRGDRLDRARTPATKRSFSKEVEERSRERPSQPEGLRKAASLTEDRDRGRDAVKREAALPPVSPLKAALSEEELEKKSKAIIEEYLHLNDMKEAVQCVQELASPSLLFIFVRHGVESTLERSAIAREHMGQLLHQLLCAGHLSTAQYYQGLYEILELAEDMEIDIPHVWLYLAELVTPILQEGGVPMGELFREITKPLRPLGKAASLLLEILGLLCKSMGPKKVGTLWREAGLSWKEFLPEGQDIGAFVAEQKVEYTLGEESEAPGQRALPSEELNRQLEKLLKEGSSNQRVFDWIEANLSEQQIVSNTLVRALMTAVCYSAIIFETPLRVDVAVLKARAKLLQKYLCDEQKELQALYALQALVVTLEQPPNLLRMFFDALYDEDVVKEDAFYSWESSKDPAEQQGKGVALKSVTAFFKWLREAEEESDHN

Target ORF information:

RefSeq Version	NM_198244.2
Organism	Homo sapiens(human)
Definition	Homo sapiens eukaryotic translation initiation factor 4 gamma 1 (EIF4G1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_198244.2

ORF Insert Sequence:

ATGATGATCC CTTCCCAGAT CTCCTACCCA GCCTCCCAGG GGGCCTACTA CATCCCTGGA 
CAGGGGCGTT CCACATACGT TGTCCCGACA CAGCAGTACC CTGTGCAGCC AGGAGCCCCA 
GGCTTCTATC CAGGTGCAAG CCCTACAGAA TTTGGGACCT ACGCTGGCGC CTACTATCCA 
GCCCAAGGGG TGCAGCAGTT TCCCACTGGC GTGGCCCCCG CCCCAGTTTT GATGAACCAG 
CCACCCCAGA TTGCTCCCAA GAGGGAGCGT AAGACGATCC GAATTCGAGA TCCAAACCAA 
GGAGGAAAGG ATATCACAGA GGAGATCATG TCTGGGGCCC GCACTGCCTC CACACCCACC 
CCTCCCCAGA CGGGAGGCGG TCTGGAGCCT CAAGCTAATG GGGAGACGCC CCAGGTTGCT 
GTCATTGTCC GGCCAGATGA CCGGTCACAG GGAGCAATCA TTGCTGACCG GCCAGGGCTG 
CCTGGCCCAG AGCATAGCCC TTCAGAATCC CAGCCTTCGT CGCCTTCTCC GACCCCATCA 
CCATCCCCAG TCTTGGAACC GGGGTCTGAG CCTAATCTCG CAGTCCTCTC TATTCCTGGG 
GACACTATGA CAACTATACA AATGTCTGTA GAAGAATCAA CCCCCATCTC CCGTGAAACT 
GGGGAGCCAT ATCGCCTCTC TCCAGAACCC ACTCCTCTCG CCGAACCCAT ACTGGAAGTA 
GAAGTGACAC TTAGCAAACC GGTTCCAGAA TCTGAGTTTT CTTCCAGTCC TCTCCAGGCT 
CCCACCCCTT TGGCATCTCA CACAGTGGAA ATTCATGAGC CTAATGGCAT GGTCCCATCT 
GAAGATCTGG AACCAGAGGT GGAGTCAAGC CCAGAGCTTG CTCCTCCCCC AGCTTGCCCC 
TCCGAATCCC CTGTGCCCAT TGCTCCAACT GCCCAACCTG AGGAACTGCT CAACGGAGCC 
CCCTCGCCAC CAGCTGTGGA CTTAAGCCCA GTCAGTGAGC CAGAGGAGCA GGCCAAGGAG 
GTGACAGCAT CAATGGCGCC CCCCACCATC CCCTCTGCTA CTCCAGCTAC GGCTCCTTCA 
GCTACTTCCC CAGCTCAGGA GGAGGAAATG GAAGAAGAAG AAGAAGAGGA AGAAGGAGAA 
GCAGGAGAAG CAGGAGAAGC TGAGAGTGAG AAAGGAGGAG AGGAACTGCT CCCCCCAGAG 
AGTACCCCTA TTCCAGCCAA CTTGTCTCAG AATTTGGAGG CAGCAGCAGC CACTCAAGTG 
GCAGTATCTG TGCCAAAGAG GAGACGGAAA ATTAAGGAGC TAAATAAGAA GGAGGCTGTT 
GGAGACCTTC TGGATGCCTT CAAGGAGGCG AACCCGGCAG TACCAGAGGT GGAAAATCAG 
CCTCCTGCAG GCAGCAATCC AGGCCCAGAG TCTGAGGGCA GTGGTGTGCC CCCACGTCCT 
GAGGAAGCAG ATGAGACCTG GGACTCAAAG GAAGACAAAA TTCACAATGC TGAGAACATC 
CAGCCCGGGG AACAGAAGTA TGAATATAAG TCAGATCAGT GGAAGCCTCT AAACCTAGAG 
GAGAAAAAAC GTTACGACCG TGAGTTCCTG CTTGGTTTTC AGTTCATCTT TGCCAGTATG 
CAGAAGCCAG AGGGATTGCC ACATATCAGT GACGTGGTGC TGGACAAGGC CAATAAAACA 
CCACTGCGGC CACTGGATCC CACTAGACTA CAAGGCATAA ATTGTGGCCC AGACTTCACT 
CCATCCTTTG CCAACCTTGG CCGGACAACC CTTAGCACCC GTGGGCCCCC AAGGGGTGGG 
CCAGGTGGGG AGCTGCCCCG TGGGCCGGCT GGCCTGGGAC CCCGGCGCTC TCAGCAGGGA 
CCCCGAAAAG AACCACGCAA GATCATTGCC ACAGTGTTAA TGACCGAAGA TATAAAACTG 
AACAAAGCAG AGAAAGCCTG GAAACCCAGC AGCAAGCGGA CGGCGGCTGA TAAGGATCGA 
GGGGAAGAAG ATGCTGATGG CAGCAAAACC CAGGACCTAT TCCGCAGGGT GCGCTCCATC 
CTGAATAAAC TGACACCCCA GATGTTCCAG CAGCTGATGA AGCAAGTGAC GCAGCTGGCC 
ATCGACACCG AGGAACGCCT CAAAGGGGTC ATTGACCTCA TTTTTGAGAA GGCCATTTCA 
GAGCCCAACT TCTCTGTGGC CTATGCCAAC ATGTGCCGCT GCCTCATGGC GCTGAAAGTG 
CCCACTACGG AAAAGCCAAC AGTGACTGTG AACTTCCGAA AGCTGTTGTT GAATCGATGT 
CAGAAGGAGT TTGAGAAAGA CAAAGATGAT GATGAGGTTT TTGAGAAGAA GCAAAAAGAG 
ATGGATGAAG CTGCTACGGC AGAGGAACGA GGACGCCTGA AGGAAGAGCT GGAAGAGGCT 
CGGGACATAG CCCGGCGGCG CTCTTTAGGG AATATCAAGT TTATTGGAGA GTTGTTCAAA 
CTGAAGATGT TAACAGAGGC AATAATGCAT GACTGTGTGG TCAAACTGCT TAAGAACCAT 
GATGAAGAGT CCCTTGAGTG CCTTTGTCGT CTGCTCACCA CCATTGGCAA AGACCTGGAC 
TTTGAAAAAG CCAAGCCCCG AATGGATCAG TATTTCAACC AGATGGAAAA AATCATTAAA 
GAAAAGAAGA CGTCATCCCG CATCCGCTTT ATGCTGCAGG ACGTGCTGGA TCTGCGAGGG 
AGCAATTGGG TGCCACGCCG AGGGGATCAG GGTCCCAAGA CCATTGACCA GATCCATAAG 
GAGGCTGAGA TGGAAGAACA TCGAGAGCAC ATCAAAGTGC AGCAGCTCAT GGCCAAGGGC 
AGTGACAAGC GTCGGGGCGG TCCTCCAGGC CCTCCCATCA GCCGTGGACT TCCCCTTGTG 
GATGATGGTG GCTGGAACAC AGTTCCCATC AGCAAAGGTA GCCGCCCCAT TGACACCTCA 
CGACTCACCA AGATCACCAA GCCTGGCTCC ATCGATTCTA ACAACCAGCT CTTTGCACCT 
GGAGGGCGAC TGAGCTGGGG CAAGGGCAGC AGCGGAGGCT CAGGAGCCAA GCCCTCAGAC 
GCAGCATCAG AAGCTGCTCG CCCAGCTACT AGTACTTTGA ATCGCTTCTC AGCCCTTCAA 
CAAGCGGTAC CCACAGAAAG CACAGATAAT AGACGTGTGG TGCAGAGGAG TAGCTTGAGC 
CGAGAACGAG GCGAGAAAGC TGGAGACCGA GGAGACCGCC TAGAGCGGAG TGAACGGGGA 
GGGGACCGTG GGGACCGGCT TGATCGTGCG CGGACACCTG CTACCAAGCG GAGCTTCAGC 
AAGGAAGTGG AGGAGCGGAG TAGAGAACGG CCCTCCCAGC CTGAGGGGCT GCGCAAGGCA 
GCTAGCCTCA CGGAGGATCG GGACCGTGGG CGGGATGCCG TGAAGCGAGA AGCTGCCCTA 
CCCCCAGTGA GCCCCCTGAA GGCGGCTCTC TCTGAGGAGG AGTTAGAGAA GAAATCCAAG 
GCTATCATTG AGGAATATCT CCATCTCAAT GACATGAAAG AGGCAGTCCA GTGCGTGCAG 
GAGCTGGCCT CACCCTCCTT GCTCTTCATC TTTGTACGGC ATGGTGTCGA GTCTACGCTG 
GAGCGCAGTG CCATTGCTCG TGAGCATATG GGGCAGCTGC TGCACCAGCT GCTCTGTGCT 
GGGCATCTGT CTACTGCTCA GTACTACCAA GGGTTGTATG AAATCTTGGA ATTGGCTGAG 
GACATGGAAA TTGACATCCC CCACGTGTGG CTCTACCTAG CGGAACTGGT AACACCCATT 
CTGCAGGAAG GTGGGGTGCC CATGGGGGAG CTGTTCAGGG AGATTACAAA GCCTCTGAGA 
CCGTTGGGCA AAGCTGCTTC CCTGTTGCTG GAGATCCTGG GCCTCCTGTG CAAAAGCATG 
GGTCCTAAAA AGGTGGGGAC GCTGTGGCGA GAAGCCGGGC TTAGCTGGAA GGAATTTCTA 
CCTGAAGGCC AGGACATTGG TGCATTCGTC GCTGAACAGA AGGTGGAGTA TACCCTGGGA 
GAGGAGTCGG AAGCCCCTGG CCAGAGGGCA CTCCCCTCCG AGGAGCTGAA CAGGCAGCTG 
GAGAAGCTGC TGAAGGAGGG CAGCAGTAAC CAGCGGGTGT TCGACTGGAT AGAGGCCAAC 
CTGAGTGAGC AGCAGATAGT ATCCAACACG TTAGTTCGAG CCCTCATGAC GGCTGTCTGC 
TATTCTGCAA TTATTTTTGA GACTCCCCTC CGAGTGGACG TTGCAGTGCT GAAAGCGCGA 
GCGAAGCTGC TGCAGAAATA CCTGTGTGAC GAGCAGAAGG AGCTACAGGC GCTCTACGCC 
CTCCAGGCCC TTGTAGTGAC CTTAGAACAG CCTCCCAACC TGCTGCGGAT GTTCTTTGAC 
GCACTGTATG ACGAGGACGT GGTGAAGGAG GATGCCTTCT ACAGTTGGGA GAGTAGCAAG 
GACCCCGCTG AGCAGCAGGG CAAGGGTGTG GCCCTTAAAT CTGTCACAGC CTTCTTCAAG 
TGGCTCCGTG AAGCAGAGGA GGAGTCTGAC CACAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EIF4G1 ( NM_001291157.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001278086.1 Homo sapiens eukaryotic translation initiation factor 4 gamma 1 (EIF4G1), transcript variant 8, mRNA.

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CloneID	OHu15300
Clone ID Related Accession (Same CDS sequence)	NM_001291157.1
Accession Version	NM_001291157.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4680bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-03-03
Organism	Homo sapiens(human)
Product	eukaryotic translation initiation factor 4 gamma 1 isoform 7
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC369776.1, AK226160.1 and BG177454.1. Transcript Variant: This variant (8) differs in the 5' UTR, includes an additional internal segment in the 5' region, which results in a downstream start codon, and uses an alternate in-frame splice site in the central coding region, compared to variant 1. The resulting isoform (7) is shorter at the N-terminus, and lacks an internal residue, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK226160.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END##

ATGAACACGC CTTCTCAGCC CCGCCAGCAC TTCTACCCTA GCCGGGCCCA GCCCCCGAGC 
AGTGCAGCCT CCCGAGTGCA GAGTGCAGCC CCTGCCCGCC CTGGCCCAGC TGCCCATGTC 
TACCCTGCTG GATCCCAAGT AATGATGATC CCTTCCCAGA TCTCCTACCC AGCCTCCCAG 
GGGGCCTACT ACATCCCTGG ACAGGGGCGT TCCACATACG TTGTCCCGAC ACAGCAGTAC 
CCTGTGCAGC CAGGAGCCCC AGGCTTCTAT CCAGGTGCAA GCCCTACAGA ATTTGGGACC 
TACGCTGGCG CCTACTATCC AGCCCAAGGG GTGCAGCAGT TTCCCACTGG CGTGGCCCCC 
GCCCCAGTTT TGATGAACCA GCCACCCCAG ATTGCTCCCA AGAGGGAGCG TAAGACGATC 
CGAATTCGAG ATCCAAACCA AGGAGGAAAG GATATCACAG AGGAGATCAT GTCTGGGGCC 
CGCACTGCCT CCACACCCAC CCCTCCCCAG ACGGGAGGCG GTCTGGAGCC TCAAGCTAAT 
GGGGAGACGC CCCAGGTTGC TGTCATTGTC CGGCCAGATG ACCGGTCACA GGGAGCAATC 
ATTGCTGACC GGCCAGGGCT GCCTGGCCCA GAGCATAGCC CTTCAGAATC CCAGCCTTCG 
TCGCCTTCTC CGACCCCATC ACCATCCCCA GTCTTGGAAC CGGGGTCTGA GCCTAATCTC 
GCAGTCCTCT CTATTCCTGG GGACACTATG ACAACTATAC AAATGTCTGT AGAAGAATCA 
ACCCCCATCT CCCGTGAAAC TGGGGAGCCA TATCGCCTCT CTCCAGAACC CACTCCTCTC 
GCCGAACCCA TACTGGAAGT AGAAGTGACA CTTAGCAAAC CGGTTCCAGA ATCTGAGTTT 
TCTTCCAGTC CTCTCCAGGC TCCCACCCCT TTGGCATCTC ACACAGTGGA AATTCATGAG 
CCTAATGGCA TGGTCCCATC TGAAGATCTG GAACCAGAGG TGGAGTCAAG CCCAGAGCTT 
GCTCCTCCCC CAGCTTGCCC CTCCGAATCC CCTGTGCCCA TTGCTCCAAC TGCCCAACCT 
GAGGAACTGC TCAACGGAGC CCCCTCGCCA CCAGCTGTGG ACTTAAGCCC AGTCAGTGAG 
CCAGAGGAGC AGGCCAAGGA GGTGACAGCA TCAATGGCGC CCCCCACCAT CCCCTCTGCT 
ACTCCAGCTA CGGCTCCTTC AGCTACTTCC CCAGCTCAGG AGGAGGAAAT GGAAGAAGAA 
GAAGAAGAGG AAGAAGGAGA AGCAGGAGAA GCAGGAGAAG CTGAGAGTGA GAAAGGAGGA 
GAGGAACTGC TCCCCCCAGA GAGTACCCCT ATTCCAGCCA ACTTGTCTCA GAATTTGGAG 
GCAGCAGCAG CCACTCAAGT GGCAGTATCT GTGCCAAAGA GGAGACGGAA AATTAAGGAG 
CTAAATAAGA AGGAGGCTGT TGGAGACCTT CTGGATGCCT TCAAGGAGGC GAACCCGGCA 
GTACCAGAGG TGGAAAATCA GCCTCCTGCA GGCAGCAATC CAGGCCCAGA GTCTGAGGGC 
AGTGGTGTGC CCCCACGTCC TGAGGAAGCA GATGAGACCT GGGACTCAAA GGAAGACAAA 
ATTCACAATG CTGAGAACAT CCAGCCCGGG GAACAGAAGT ATGAATATAA GTCAGATCAG 
TGGAAGCCTC TAAACCTAGA GGAGAAAAAA CGTTACGACC GTGAGTTCCT GCTTGGTTTT 
CAGTTCATCT TTGCCAGTAT GCAGAAGCCA GAGGGATTGC CACATATCAG TGACGTGGTG 
CTGGACAAGG CCAATAAAAC ACCACTGCGG CCACTGGATC CCACTAGACT ACAAGGCATA 
AATTGTGGCC CAGACTTCAC TCCATCCTTT GCCAACCTTG GCCGGACAAC CCTTAGCACC 
CGTGGGCCCC CAAGGGGTGG GCCAGGTGGG GAGCTGCCCC GTGGGCCGGC TGGCCTGGGA 
CCCCGGCGCT CTCAGCAGGG ACCCCGAAAA GAACCACGCA AGATCATTGC CACAGTGTTA 
ATGACCGAAG ATATAAAACT GAACAAAGCA GAGAAAGCCT GGAAACCCAG CAGCAAGCGG 
ACGGCGGCTG ATAAGGATCG AGGGGAAGAA GATGCTGATG GCAGCAAAAC CCAGGACCTA 
TTCCGCAGGG TGCGCTCCAT CCTGAATAAA CTGACACCCC AGATGTTCCA GCAGCTGATG 
AAGCAAGTGA CGCAGCTGGC CATCGACACC GAGGAACGCC TCAAAGGGGT CATTGACCTC 
ATTTTTGAGA AGGCCATTTC AGAGCCCAAC TTCTCTGTGG CCTATGCCAA CATGTGCCGC 
TGCCTCATGG CGCTGAAAGT GCCCACTACG GAAAAGCCAA CAGTGACTGT GAACTTCCGA 
AAGCTGTTGT TGAATCGATG TCAGAAGGAG TTTGAGAAAG ACAAAGATGA TGATGAGGTT 
TTTGAGAAGA AGCAAAAAGA GATGGATGAA GCTGCTACGG CAGAGGAACG AGGACGCCTG 
AAGGAAGAGC TGGAAGAGGC TCGGGACATA GCCCGGCGGC GCTCTTTAGG GAATATCAAG 
TTTATTGGAG AGTTGTTCAA ACTGAAGATG TTAACAGAGG CAATAATGCA TGACTGTGTG 
GTCAAACTGC TTAAGAACCA TGATGAAGAG TCCCTTGAGT GCCTTTGTCG TCTGCTCACC 
ACCATTGGCA AAGACCTGGA CTTTGAAAAA GCCAAGCCCC GAATGGATCA GTATTTCAAC 
CAGATGGAAA AAATCATTAA AGAAAAGAAG ACGTCATCCC GCATCCGCTT TATGCTGCAG 
GACGTGCTGG ATCTGCGAGG GAGCAATTGG GTGCCACGCC GAGGGGATCA GGGTCCCAAG 
ACCATTGACC AGATCCATAA GGAGGCTGAG ATGGAAGAAC ATCGAGAGCA CATCAAAGTG 
CAGCAGCTCA TGGCCAAGGG CAGTGACAAG CGTCGGGGCG GTCCTCCAGG CCCTCCCATC 
AGCCGTGGAC TTCCCCTTGT GGATGATGGT GGCTGGAACA CAGTTCCCAT CAGCAAAGGT 
AGCCGCCCCA TTGACACCTC ACGACTCACC AAGATCACCA AGCCTGGCTC CATCGATTCT 
AACAACCAGC TCTTTGCACC TGGAGGGCGA CTGAGCTGGG GCAAGGGCAG CAGCGGAGGC 
TCAGGAGCCA AGCCCTCAGA CGCAGCATCA GAAGCTGCTC GCCCAGCTAC TAGTACTTTG 
AATCGCTTCT CAGCCCTTCA ACAAGCGGTA CCCACAGAAA GCACAGATAA TAGACGTGTG 
GTGCAGAGGA GTAGCTTGAG CCGAGAACGA GGCGAGAAAG CTGGAGACCG AGGAGACCGC 
CTAGAGCGGA GTGAACGGGG AGGGGACCGT GGGGACCGGC TTGATCGTGC GCGGACACCT 
GCTACCAAGC GGAGCTTCAG CAAGGAAGTG GAGGAGCGGA GTAGAGAACG GCCCTCCCAG 
CCTGAGGGGC TGCGCAAGGC AGCTAGCCTC ACGGAGGATC GGGACCGTGG GCGGGATGCC 
GTGAAGCGAG AAGCTGCCCT ACCCCCAGTG AGCCCCCTGA AGGCGGCTCT CTCTGAGGAG 
GAGTTAGAGA AGAAATCCAA GGCTATCATT GAGGAATATC TCCATCTCAA TGACATGAAA 
GAGGCAGTCC AGTGCGTGCA GGAGCTGGCC TCACCCTCCT TGCTCTTCAT CTTTGTACGG 
CATGGTGTCG AGTCTACGCT GGAGCGCAGT GCCATTGCTC GTGAGCATAT GGGGCAGCTG 
CTGCACCAGC TGCTCTGTGC TGGGCATCTG TCTACTGCTC AGTACTACCA AGGGTTGTAT 
GAAATCTTGG AATTGGCTGA GGACATGGAA ATTGACATCC CCCACGTGTG GCTCTACCTA 
GCGGAACTGG TAACACCCAT TCTGCAGGAA GGTGGGGTGC CCATGGGGGA GCTGTTCAGG 
GAGATTACAA AGCCTCTGAG ACCGTTGGGC AAAGCTGCTT CCCTGTTGCT GGAGATCCTG 
GGCCTCCTGT GCAAAAGCAT GGGTCCTAAA AAGGTGGGGA CGCTGTGGCG AGAAGCCGGG 
CTTAGCTGGA AGGAATTTCT ACCTGAAGGC CAGGACATTG GTGCATTCGT CGCTGAACAG 
AAGGTGGAGT ATACCCTGGG AGAGGAGTCG GAAGCCCCTG GCCAGAGGGC ACTCCCCTCC 
GAGGAGCTGA ACAGGCAGCT GGAGAAGCTG CTGAAGGAGG GCAGCAGTAA CCAGCGGGTG 
TTCGACTGGA TAGAGGCCAA CCTGAGTGAG CAGCAGATAG TATCCAACAC GTTAGTTCGA 
GCCCTCATGA CGGCTGTCTG CTATTCTGCA ATTATTTTTG AGACTCCCCT CCGAGTGGAC 
GTTGCAGTGC TGAAAGCGCG AGCGAAGCTG CTGCAGAAAT ACCTGTGTGA CGAGCAGAAG 
GAGCTACAGG CGCTCTACGC CCTCCAGGCC CTTGTAGTGA CCTTAGAACA GCCTCCCAAC 
CTGCTGCGGA TGTTCTTTGA CGCACTGTAT GACGAGGACG TGGTGAAGGA GGATGCCTTC 
TACAGTTGGG AGAGTAGCAA GGACCCCGCT GAGCAGCAGG GCAAGGGTGT GGCCCTTAAA 
TCTGTCACAG CCTTCTTCAA GTGGCTCCGT GAAGCAGAGG AGGAGTCTGA CCACAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001278086.1
CDS	445..5124
Translation	MNTPSQPRQHFYPSRAQPPSSAASRVQSAAPARPGPAAHVYPAGSQVMMIPSQISYPASQGAYYIPGQGRSTYVVPTQQYPVQPGAPGFYPGASPTEFGTYAGAYYPAQGVQQFPTGVAPAPVLMNQPPQIAPKRERKTIRIRDPNQGGKDITEEIMSGARTASTPTPPQTGGGLEPQANGETPQVAVIVRPDDRSQGAIIADRPGLPGPEHSPSESQPSSPSPTPSPSPVLEPGSEPNLAVLSIPGDTMTTIQMSVEESTPISRETGEPYRLSPEPTPLAEPILEVEVTLSKPVPESEFSSSPLQAPTPLASHTVEIHEPNGMVPSEDLEPEVESSPELAPPPACPSESPVPIAPTAQPEELLNGAPSPPAVDLSPVSEPEEQAKEVTASMAPPTIPSATPATAPSATSPAQEEEMEEEEEEEEGEAGEAGEAESEKGGEELLPPESTPIPANLSQNLEAAAATQVAVSVPKRRRKIKELNKKEAVGDLLDAFKEANPAVPEVENQPPAGSNPGPESEGSGVPPRPEEADETWDSKEDKIHNAENIQPGEQKYEYKSDQWKPLNLEEKKRYDREFLLGFQFIFASMQKPEGLPHISDVVLDKANKTPLRPLDPTRLQGINCGPDFTPSFANLGRTTLSTRGPPRGGPGGELPRGPAGLGPRRSQQGPRKEPRKIIATVLMTEDIKLNKAEKAWKPSSKRTAADKDRGEEDADGSKTQDLFRRVRSILNKLTPQMFQQLMKQVTQLAIDTEERLKGVIDLIFEKAISEPNFSVAYANMCRCLMALKVPTTEKPTVTVNFRKLLLNRCQKEFEKDKDDDEVFEKKQKEMDEAATAEERGRLKEELEEARDIARRRSLGNIKFIGELFKLKMLTEAIMHDCVVKLLKNHDEESLECLCRLLTTIGKDLDFEKAKPRMDQYFNQMEKIIKEKKTSSRIRFMLQDVLDLRGSNWVPRRGDQGPKTIDQIHKEAEMEEHREHIKVQQLMAKGSDKRRGGPPGPPISRGLPLVDDGGWNTVPISKGSRPIDTSRLTKITKPGSIDSNNQLFAPGGRLSWGKGSSGGSGAKPSDAASEAARPATSTLNRFSALQQAVPTESTDNRRVVQRSSLSRERGEKAGDRGDRLERSERGGDRGDRLDRARTPATKRSFSKEVEERSRERPSQPEGLRKAASLTEDRDRGRDAVKREAALPPVSPLKAALSEEELEKKSKAIIEEYLHLNDMKEAVQCVQELASPSLLFIFVRHGVESTLERSAIAREHMGQLLHQLLCAGHLSTAQYYQGLYEILELAEDMEIDIPHVWLYLAELVTPILQEGGVPMGELFREITKPLRPLGKAASLLLEILGLLCKSMGPKKVGTLWREAGLSWKEFLPEGQDIGAFVAEQKVEYTLGEESEAPGQRALPSEELNRQLEKLLKEGSSNQRVFDWIEANLSEQQIVSNTLVRALMTAVCYSAIIFETPLRVDVAVLKARAKLLQKYLCDEQKELQALYALQALVVTLEQPPNLLRMFFDALYDEDVVKEDAFYSWESSKDPAEQQGKGVALKSVTAFFKWLREAEEESDHN

Target ORF information:

RefSeq Version	NM_001291157.1
Organism	Homo sapiens(human)
Definition	Homo sapiens eukaryotic translation initiation factor 4 gamma 1 (EIF4G1), transcript variant 8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001291157.1

ORF Insert Sequence:

ATGAACACGC CTTCTCAGCC CCGCCAGCAC TTCTACCCTA GCCGGGCCCA GCCCCCGAGC 
AGTGCAGCCT CCCGAGTGCA GAGTGCAGCC CCTGCCCGCC CTGGCCCAGC TGCCCATGTC 
TACCCTGCTG GATCCCAAGT AATGATGATC CCTTCCCAGA TCTCCTACCC AGCCTCCCAG 
GGGGCCTACT ACATCCCTGG ACAGGGGCGT TCCACATACG TTGTCCCGAC ACAGCAGTAC 
CCTGTGCAGC CAGGAGCCCC AGGCTTCTAT CCAGGTGCAA GCCCTACAGA ATTTGGGACC 
TACGCTGGCG CCTACTATCC AGCCCAAGGG GTGCAGCAGT TTCCCACTGG CGTGGCCCCC 
GCCCCAGTTT TGATGAACCA GCCACCCCAG ATTGCTCCCA AGAGGGAGCG TAAGACGATC 
CGAATTCGAG ATCCAAACCA AGGAGGAAAG GATATCACAG AGGAGATCAT GTCTGGGGCC 
CGCACTGCCT CCACACCCAC CCCTCCCCAG ACGGGAGGCG GTCTGGAGCC TCAAGCTAAT 
GGGGAGACGC CCCAGGTTGC TGTCATTGTC CGGCCAGATG ACCGGTCACA GGGAGCAATC 
ATTGCTGACC GGCCAGGGCT GCCTGGCCCA GAGCATAGCC CTTCAGAATC CCAGCCTTCG 
TCGCCTTCTC CGACCCCATC ACCATCCCCA GTCTTGGAAC CGGGGTCTGA GCCTAATCTC 
GCAGTCCTCT CTATTCCTGG GGACACTATG ACAACTATAC AAATGTCTGT AGAAGAATCA 
ACCCCCATCT CCCGTGAAAC TGGGGAGCCA TATCGCCTCT CTCCAGAACC CACTCCTCTC 
GCCGAACCCA TACTGGAAGT AGAAGTGACA CTTAGCAAAC CGGTTCCAGA ATCTGAGTTT 
TCTTCCAGTC CTCTCCAGGC TCCCACCCCT TTGGCATCTC ACACAGTGGA AATTCATGAG 
CCTAATGGCA TGGTCCCATC TGAAGATCTG GAACCAGAGG TGGAGTCAAG CCCAGAGCTT 
GCTCCTCCCC CAGCTTGCCC CTCCGAATCC CCTGTGCCCA TTGCTCCAAC TGCCCAACCT 
GAGGAACTGC TCAACGGAGC CCCCTCGCCA CCAGCTGTGG ACTTAAGCCC AGTCAGTGAG 
CCAGAGGAGC AGGCCAAGGA GGTGACAGCA TCAATGGCGC CCCCCACCAT CCCCTCTGCT 
ACTCCAGCTA CGGCTCCTTC AGCTACTTCC CCAGCTCAGG AGGAGGAAAT GGAAGAAGAA 
GAAGAAGAGG AAGAAGGAGA AGCAGGAGAA GCAGGAGAAG CTGAGAGTGA GAAAGGAGGA 
GAGGAACTGC TCCCCCCAGA GAGTACCCCT ATTCCAGCCA ACTTGTCTCA GAATTTGGAG 
GCAGCAGCAG CCACTCAAGT GGCAGTATCT GTGCCAAAGA GGAGACGGAA AATTAAGGAG 
CTAAATAAGA AGGAGGCTGT TGGAGACCTT CTGGATGCCT TCAAGGAGGC GAACCCGGCA 
GTACCAGAGG TGGAAAATCA GCCTCCTGCA GGCAGCAATC CAGGCCCAGA GTCTGAGGGC 
AGTGGTGTGC CCCCACGTCC TGAGGAAGCA GATGAGACCT GGGACTCAAA GGAAGACAAA 
ATTCACAATG CTGAGAACAT CCAGCCCGGG GAACAGAAGT ATGAATATAA GTCAGATCAG 
TGGAAGCCTC TAAACCTAGA GGAGAAAAAA CGTTACGACC GTGAGTTCCT GCTTGGTTTT 
CAGTTCATCT TTGCCAGTAT GCAGAAGCCA GAGGGATTGC CACATATCAG TGACGTGGTG 
CTGGACAAGG CCAATAAAAC ACCACTGCGG CCACTGGATC CCACTAGACT ACAAGGCATA 
AATTGTGGCC CAGACTTCAC TCCATCCTTT GCCAACCTTG GCCGGACAAC CCTTAGCACC 
CGTGGGCCCC CAAGGGGTGG GCCAGGTGGG GAGCTGCCCC GTGGGCCGGC TGGCCTGGGA 
CCCCGGCGCT CTCAGCAGGG ACCCCGAAAA GAACCACGCA AGATCATTGC CACAGTGTTA 
ATGACCGAAG ATATAAAACT GAACAAAGCA GAGAAAGCCT GGAAACCCAG CAGCAAGCGG 
ACGGCGGCTG ATAAGGATCG AGGGGAAGAA GATGCTGATG GCAGCAAAAC CCAGGACCTA 
TTCCGCAGGG TGCGCTCCAT CCTGAATAAA CTGACACCCC AGATGTTCCA GCAGCTGATG 
AAGCAAGTGA CGCAGCTGGC CATCGACACC GAGGAACGCC TCAAAGGGGT CATTGACCTC 
ATTTTTGAGA AGGCCATTTC AGAGCCCAAC TTCTCTGTGG CCTATGCCAA CATGTGCCGC 
TGCCTCATGG CGCTGAAAGT GCCCACTACG GAAAAGCCAA CAGTGACTGT GAACTTCCGA 
AAGCTGTTGT TGAATCGATG TCAGAAGGAG TTTGAGAAAG ACAAAGATGA TGATGAGGTT 
TTTGAGAAGA AGCAAAAAGA GATGGATGAA GCTGCTACGG CAGAGGAACG AGGACGCCTG 
AAGGAAGAGC TGGAAGAGGC TCGGGACATA GCCCGGCGGC GCTCTTTAGG GAATATCAAG 
TTTATTGGAG AGTTGTTCAA ACTGAAGATG TTAACAGAGG CAATAATGCA TGACTGTGTG 
GTCAAACTGC TTAAGAACCA TGATGAAGAG TCCCTTGAGT GCCTTTGTCG TCTGCTCACC 
ACCATTGGCA AAGACCTGGA CTTTGAAAAA GCCAAGCCCC GAATGGATCA GTATTTCAAC 
CAGATGGAAA AAATCATTAA AGAAAAGAAG ACGTCATCCC GCATCCGCTT TATGCTGCAG 
GACGTGCTGG ATCTGCGAGG GAGCAATTGG GTGCCACGCC GAGGGGATCA GGGTCCCAAG 
ACCATTGACC AGATCCATAA GGAGGCTGAG ATGGAAGAAC ATCGAGAGCA CATCAAAGTG 
CAGCAGCTCA TGGCCAAGGG CAGTGACAAG CGTCGGGGCG GTCCTCCAGG CCCTCCCATC 
AGCCGTGGAC TTCCCCTTGT GGATGATGGT GGCTGGAACA CAGTTCCCAT CAGCAAAGGT 
AGCCGCCCCA TTGACACCTC ACGACTCACC AAGATCACCA AGCCTGGCTC CATCGATTCT 
AACAACCAGC TCTTTGCACC TGGAGGGCGA CTGAGCTGGG GCAAGGGCAG CAGCGGAGGC 
TCAGGAGCCA AGCCCTCAGA CGCAGCATCA GAAGCTGCTC GCCCAGCTAC TAGTACTTTG 
AATCGCTTCT CAGCCCTTCA ACAAGCGGTA CCCACAGAAA GCACAGATAA TAGACGTGTG 
GTGCAGAGGA GTAGCTTGAG CCGAGAACGA GGCGAGAAAG CTGGAGACCG AGGAGACCGC 
CTAGAGCGGA GTGAACGGGG AGGGGACCGT GGGGACCGGC TTGATCGTGC GCGGACACCT 
GCTACCAAGC GGAGCTTCAG CAAGGAAGTG GAGGAGCGGA GTAGAGAACG GCCCTCCCAG 
CCTGAGGGGC TGCGCAAGGC AGCTAGCCTC ACGGAGGATC GGGACCGTGG GCGGGATGCC 
GTGAAGCGAG AAGCTGCCCT ACCCCCAGTG AGCCCCCTGA AGGCGGCTCT CTCTGAGGAG 
GAGTTAGAGA AGAAATCCAA GGCTATCATT GAGGAATATC TCCATCTCAA TGACATGAAA 
GAGGCAGTCC AGTGCGTGCA GGAGCTGGCC TCACCCTCCT TGCTCTTCAT CTTTGTACGG 
CATGGTGTCG AGTCTACGCT GGAGCGCAGT GCCATTGCTC GTGAGCATAT GGGGCAGCTG 
CTGCACCAGC TGCTCTGTGC TGGGCATCTG TCTACTGCTC AGTACTACCA AGGGTTGTAT 
GAAATCTTGG AATTGGCTGA GGACATGGAA ATTGACATCC CCCACGTGTG GCTCTACCTA 
GCGGAACTGG TAACACCCAT TCTGCAGGAA GGTGGGGTGC CCATGGGGGA GCTGTTCAGG 
GAGATTACAA AGCCTCTGAG ACCGTTGGGC AAAGCTGCTT CCCTGTTGCT GGAGATCCTG 
GGCCTCCTGT GCAAAAGCAT GGGTCCTAAA AAGGTGGGGA CGCTGTGGCG AGAAGCCGGG 
CTTAGCTGGA AGGAATTTCT ACCTGAAGGC CAGGACATTG GTGCATTCGT CGCTGAACAG 
AAGGTGGAGT ATACCCTGGG AGAGGAGTCG GAAGCCCCTG GCCAGAGGGC ACTCCCCTCC 
GAGGAGCTGA ACAGGCAGCT GGAGAAGCTG CTGAAGGAGG GCAGCAGTAA CCAGCGGGTG 
TTCGACTGGA TAGAGGCCAA CCTGAGTGAG CAGCAGATAG TATCCAACAC GTTAGTTCGA 
GCCCTCATGA CGGCTGTCTG CTATTCTGCA ATTATTTTTG AGACTCCCCT CCGAGTGGAC 
GTTGCAGTGC TGAAAGCGCG AGCGAAGCTG CTGCAGAAAT ACCTGTGTGA CGAGCAGAAG 
GAGCTACAGG CGCTCTACGC CCTCCAGGCC CTTGTAGTGA CCTTAGAACA GCCTCCCAAC 
CTGCTGCGGA TGTTCTTTGA CGCACTGTAT GACGAGGACG TGGTGAAGGA GGATGCCTTC 
TACAGTTGGG AGAGTAGCAA GGACCCCGCT GAGCAGCAGG GCAAGGGTGT GGCCCTTAAA 
TCTGTCACAG CCTTCTTCAA GTGGCTCCGT GAAGCAGAGG AGGAGTCTGA CCACAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	EIF4G1
Entrez Gene ID	1981
Full Name	eukaryotic translation initiation factor 4 gamma 1
Synonyms	EIF-4G1,EIF4F,EIF4G,EIF4GI,P220,PARK18
General protein information	Preferred Names eukaryotic translation initiation factor 4 gamma 1 Names eukaryotic translation initiation factor 4 gamma 1 EIF4-gamma eIF-4-gamma 1 eucaryotic translation initiation factor 4G
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 3 Map Location: 3q27.1
Summary	The protein encoded by this gene is a component of the multi-subunit protein complex EIF4F. This complex facilitates the recruitment of mRNA to the ribosome, which is a rate-limiting step during the initiation phase of protein synthesis. The recognition of the mRNA cap and the ATP-dependent unwinding of 5'-terminal secondary structure is catalyzed by factors in this complex. The subunit encoded by this gene is a large scaffolding protein that contains binding sites for other members of the EIF4F complex. A domain at its N-terminus can also interact with the poly(A)-binding protein, which may mediate the circularization of mRNA during translation. Alternative splicing results in multiple transcript variants, some of which are derived from alternative promoter usage. [provided by RefSeq, Aug 2010].
Disorder MIM:	600495
Disorder Html:	{Parkinson disease 18}, 614251 (3)

	EIF4G1	XP_002802932.1
Canis lupus familiaris (dog)	EIF4G1	XP_005640005.1
Bos taurus (cattle)	EIF4G1	XP_003585711.2
Mus musculus (house mouse)	Eif4g1	NP_666053.2
Homo sapiens (human)	EIF4G1	NP_886553.3
Rattus norvegicus (Norway rat)	Eif4g1	XP_006248643.1
Gallus gallus (chicken)	EIF4G1	XP_004943468.1
Xenopus tropicalis (tropical clawed frog)	eif4g3	XP_004914456.1
Pan troglodytes (chimpanzee)	EIF4G1	XP_003310194.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

EIF4G1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

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