EIF4EBP2 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	EIF4EBP2
Entrez Gene ID	1979
Full Name	eukaryotic translation initiation factor 4E binding protein 2
Synonyms	4EBP2,PHASII
General protein information	Preferred Names eukaryotic translation initiation factor 4E binding protein 2 Names eukaryotic translation initiation factor 4E-binding protein 2 4E-BP2 eIF4E-binding protein 2 phosphorylated, heat and acid stable regulated by insulin protein II
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 10 Map Location: 10q22.1
Summary	This gene encodes a member of the eukaryotic translation initiation factor 4E binding protein family. The gene products of this family bind eIF4E and inhibit translation initiation. However, insulin and other growth factors can release this inhibition via a phosphorylation-dependent disruption of their binding to eIF4E. Regulation of protein production through these gene products have been implicated in cell proliferation, cell differentiation and viral infection. [provided by RefSeq, Oct 2008].
Disorder MIM:	602224

mRNA and Protein(s)

mRNA	Protein	Name
NM_004096.4	NP_004087.1	eukaryotic translation initiation factor 4E-binding protein 2
NM_004096.5	NP_004087.1	eukaryotic translation initiation factor 4E-binding protein 2

Pathways from BioSystems

Homologies

	EIF4EBP2	XP_002805739.1
Mus musculus (house mouse)	Eif4ebp2	NP_034254.1
Xenopus tropicalis (tropical clawed frog)	eif4ebp2	NP_989333.1
Pan troglodytes (chimpanzee)	EIF4EBP2	XP_003312651.1
Homo sapiens (human)	EIF4EBP2	NP_004087.1
Canis lupus familiaris (dog)	EIF4EBP2	XP_546145.2
Bos taurus (cattle)	EIF4EBP2	NP_001178078.1
Rattus norvegicus (Norway rat)	Eif4ebp2	NP_001028241.1
Danio rerio (zebrafish)	eif4ebp2	NP_997968.1

Gene Ontology
Bibliography

Related articles in PubMed

Architecture of the human interactome defines protein communities and disease networks.
Huttlin EL, Bruckner RJ, Paulo JA, Cannon JR, Ting L, Baltier K, Colby G, Gebreab F, Gygi MP, Parzen H, Szpyt J, Tam S, Zarraga G, Pontano-Vaites L, Swarup S, White AE, Schweppe DK, Rad R, Erickson BK, Obar RA, Guruharsha KG, Li K, Artavanis-Tsakonas S, Gygi SP, Harper JW
Nature545(7655)505-509(2017 05)

Mechanism of Phosphorylation-Induced Folding of 4E-BP2 Revealed by Molecular Dynamics Simulations.
Zeng J, Jiang F, Wu YD
Journal of chemical theory and computation13(1)320-328(2017 01)

Oncofetal protein IGF2BP3 facilitates the activity of proto-oncogene protein eIF4E through the destabilization of EIF4E-BP2 mRNA.
Mizutani R, Imamachi N, Suzuki Y, Yoshida H, Tochigi N, Oonishi T, Suzuki Y, Akimitsu N
Oncogene35(27)3495-502(2016 Jul)

Folding of an intrinsically disordered protein by phosphorylation as a regulatory switch.
Bah A, Vernon RM, Siddiqui Z, Krzeminski M, Muhandiram R, Zhao C, Sonenberg N, Kay LE, Forman-Kay JD
Nature519(7541)106-9(2015 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

molecular dynamics simulations to investigate both the folded and unfolded states of 4E-BP2 under different phosphorylation states of T37 and T46
Title: Mechanism of Phosphorylation-Induced Folding of 4E-BP2 Revealed by Molecular Dynamics Simulations.

Results suggest that IGF2BP3 promotes eIF4E-mediated translational activation through the reduction of EIF4E-BP2 via mRNA degradation, leading to enhanced cell proliferation.
Title: Oncofetal protein IGF2BP3 facilitates the activity of proto-oncogene protein eIF4E through the destabilization of EIF4E-BP2 mRNA.

results highlight stabilization of a phosphorylation-induced fold as the essential mechanism for phospho-regulation of the 4E-BP:eIF4E interaction and exemplify a new mode of biological regulation mediated by intrinsically disordered proteins
Title: Folding of an intrinsically disordered protein by phosphorylation as a regulatory switch.

mTORC1 controls mitochondrial activity and biogenesis by selectively promoting translation of nucleus-encoded mitochondria-related mRNAs via inhibition of the eukaryotic translation initiation factor 4E (eIF4E)-binding proteins (4E-BPs).
Title: mTORC1 controls mitochondrial activity and biogenesis through 4E-BP-dependent translational regulation.

Data show that the eIF4E binding preference for 4E-BP2 over 4E-BP1 is based on stacking of Arg63 side chain on Trp73 indole ring of eIF4E and construction of hydrophobic space around the Trp73 indole ring by the Leu59-Leu60 sequence of 4E-BP2.
Title: Structural scaffold for eIF4E binding selectivity of 4E-BP isoforms: crystal structure of eIF4E binding region of 4E-BP2 and its comparison with that of 4E-BP1.

The following EIF4EBP2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EIF4EBP2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu30760	NM_004096.5 Latest version!	Homo sapiens eukaryotic translation initiation factor 4E binding protein 2 (EIF4EBP2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
View Old Accession Versions >>
OHu30760	NM_004096.4	Homo sapiens eukaryotic translation initiation factor 4E binding protein 2 (EIF4EBP2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

EIF4EBP2 ( NM_004096.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_004087.1 Homo sapiens eukaryotic translation initiation factor 4E binding protein 2 (EIF4EBP2), mRNA.

Price & Availability↑

CloneID	OHu30760
Clone ID Related Accession (Same CDS sequence)	NM_004096.4 , NM_004096.5
Accession Version	NM_004096.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 363bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	eukaryotic translation initiation factor 4E-binding protein 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BG717474.1, BC050633.1 and AC022532.13. On Oct 7, 2008 this sequence version replaced NM_004096.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.66710.1, SRR1803614.8472.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCCTCGT CAGCCGGCAG CGGCCACCAG CCCAGCCAGA GCCGCGCCAT CCCCACCCGC 
ACCGTGGCCA TCAGCGACGC CGCGCAGCTA CCTCATGACT ATTGCACCAC GCCCGGGGGG 
ACGCTCTTCT CCACCACACC GGGAGGAACT CGAATCATTT ATGACAGAAA GTTTCTGTTG 
GATCGTCGCA ATTCTCCCAT GGCTCAGACC CCACCCTGCC ACCTGCCCAA TATCCCAGGA 
GTCACTAGCC CTGGCACCTT AATTGAAGAC TCCAAAGTAG AAGTAAACAA TTTGAACAAC 
TTGAACAATC ACGACAGGAA ACATGCAGTT GGGGATGATG CTCAGTTCGA GATGGACATC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004087.1
CDS	298..660
Translation	MSSSAGSGHQPSQSRAIPTRTVAISDAAQLPHDYCTTPGGTLFSTTPGGTRIIYDRKFLLDRRNSPMAQTPPCHLPNIPGVTSPGTLIEDSKVEVNNLNNLNNHDRKHAVGDDAQFEMDI

Target ORF information:

RefSeq Version	NM_004096.4
Organism	Homo sapiens(human)
Definition	Homo sapiens eukaryotic translation initiation factor 4E binding protein 2 (EIF4EBP2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004096.4

ORF Insert Sequence:

ATGTCCTCGT CAGCCGGCAG CGGCCACCAG CCCAGCCAGA GCCGCGCCAT CCCCACCCGC 
ACCGTGGCCA TCAGCGACGC CGCGCAGCTA CCTCATGACT ATTGCACCAC GCCCGGGGGG 
ACGCTCTTCT CCACCACACC GGGAGGAACT CGAATCATTT ATGACAGAAA GTTTCTGTTG 
GATCGTCGCA ATTCTCCCAT GGCTCAGACC CCACCCTGCC ACCTGCCCAA TATCCCAGGA 
GTCACTAGCC CTGGCACCTT AATTGAAGAC TCCAAAGTAG AAGTAAACAA TTTGAACAAC 
TTGAACAATC ACGACAGGAA ACATGCAGTT GGGGATGATG CTCAGTTCGA GATGGACATC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EIF4EBP2 ( NM_004096.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_004087.1 Homo sapiens eukaryotic translation initiation factor 4E binding protein 2 (EIF4EBP2), mRNA.

Price & Availability↑

CloneID	OHu30760
Clone ID Related Accession (Same CDS sequence)	NM_004096.4 , NM_004096.5
Accession Version	NM_004096.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 363bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-16
Organism	Homo sapiens(human)
Product	eukaryotic translation initiation factor 4E-binding protein 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BG717474.1, BC050633.1 and AC022532.13. On Nov 22, 2018 this sequence version replaced NM_004096.4. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.33657.1, SRR1803611.66710.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000373218.5/ ENSP00000362314.4 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGTCCTCGT CAGCCGGCAG CGGCCACCAG CCCAGCCAGA GCCGCGCCAT CCCCACCCGC 
ACCGTGGCCA TCAGCGACGC CGCGCAGCTA CCTCATGACT ATTGCACCAC GCCCGGGGGG 
ACGCTCTTCT CCACCACACC GGGAGGAACT CGAATCATTT ATGACAGAAA GTTTCTGTTG 
GATCGTCGCA ATTCTCCCAT GGCTCAGACC CCACCCTGCC ACCTGCCCAA TATCCCAGGA 
GTCACTAGCC CTGGCACCTT AATTGAAGAC TCCAAAGTAG AAGTAAACAA TTTGAACAAC 
TTGAACAATC ACGACAGGAA ACATGCAGTT GGGGATGATG CTCAGTTCGA GATGGACATC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004087.1
CDS	258..620
Translation	MSSSAGSGHQPSQSRAIPTRTVAISDAAQLPHDYCTTPGGTLFSTTPGGTRIIYDRKFLLDRRNSPMAQTPPCHLPNIPGVTSPGTLIEDSKVEVNNLNNLNNHDRKHAVGDDAQFEMDI

Target ORF information:

RefSeq Version	NM_004096.5
Organism	Homo sapiens(human)
Definition	Homo sapiens eukaryotic translation initiation factor 4E binding protein 2 (EIF4EBP2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004096.5

ORF Insert Sequence:

ATGTCCTCGT CAGCCGGCAG CGGCCACCAG CCCAGCCAGA GCCGCGCCAT CCCCACCCGC 
ACCGTGGCCA TCAGCGACGC CGCGCAGCTA CCTCATGACT ATTGCACCAC GCCCGGGGGG 
ACGCTCTTCT CCACCACACC GGGAGGAACT CGAATCATTT ATGACAGAAA GTTTCTGTTG 
GATCGTCGCA ATTCTCCCAT GGCTCAGACC CCACCCTGCC ACCTGCCCAA TATCCCAGGA 
GTCACTAGCC CTGGCACCTT AATTGAAGAC TCCAAAGTAG AAGTAAACAA TTTGAACAAC 
TTGAACAATC ACGACAGGAA ACATGCAGTT GGGGATGATG CTCAGTTCGA GATGGACATC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Architecture of the human interactome defines protein communities and disease networks.
Nature545(7655)505-509(2017 05)
Huttlin EL,Bruckner RJ,Paulo JA,Cannon JR,Ting L,Baltier K,Colby G,Gebreab F,Gygi MP,Parzen H,Szpyt J,Tam S,Zarraga G,Pontano-Vaites L,Swarup S,White AE,Schweppe DK,Rad R,Erickson BK,Obar RA,Guruharsha KG,Li K,Artavanis-Tsakonas S,Gygi SP,Harper JW

Mechanism of Phosphorylation-Induced Folding of 4E-BP2 Revealed by Molecular Dynamics Simulations.
Journal of chemical theory and computation13(1)320-328(2017 01)
Zeng J,Jiang F,Wu YD

Oncofetal protein IGF2BP3 facilitates the activity of proto-oncogene protein eIF4E through the destabilization of EIF4E-BP2 mRNA.
Oncogene35(27)3495-502(2016 Jul)
Mizutani R,Imamachi N,Suzuki Y,Yoshida H,Tochigi N,Oonishi T,Suzuki Y,Akimitsu N

Folding of an intrinsically disordered protein by phosphorylation as a regulatory switch.
Nature519(7541)106-9(2015 Mar)
Bah A,Vernon RM,Siddiqui Z,Krzeminski M,Muhandiram R,Zhao C,Sonenberg N,Kay LE,Forman-Kay JD

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EIF4EBP2 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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