UBR1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	UBR1
Entrez Gene ID	197131
Full Name	ubiquitin protein ligase E3 component n-recognin 1
Synonyms	JBS
General protein information	Preferred Names ubiquitin protein ligase E3 component n-recognin 1 Names E3 ubiquitin-protein ligase UBR1 E3a ligase N-recognin-1 RING-type E3 ubiquitin transferase UBR1 ubiquitin ligase E3 alpha-I ubiquitin-protein ligase E3-alpha ubiquitin-protein ligase E3-alpha-1 ubiquitin-protein ligase E3-alpha-I
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 15 Map Location: 15q15.2
Summary	The N-end rule pathway is one proteolytic pathway of the ubiquitin system. The recognition component of this pathway, encoded by this gene, binds to a destabilizing N-terminal residue of a substrate protein and participates in the formation of a substrate-linked multiubiquitin chain. This leads to the eventual degradation of the substrate protein. The protein described in this record has a RING-type zinc finger and a UBR-type zinc finger. Mutations in this gene have been associated with Johanson-Blizzard syndrome. [provided by RefSeq, Jul 2008].
Disorder MIM:	605981
Disorder Html:	Johanson-Blizzard syndrome, 243800 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_174916.2	NP_777576.1	E3 ubiquitin-protein ligase UBR1
NM_174916.3	NP_777576.1	E3 ubiquitin-protein ligase UBR1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	UBR1	XP_510341.3
	UBR1	XP_001105721.2
Canis lupus familiaris (dog)	UBR1	XP_544645.2
Bos taurus (cattle)	UBR1	NP_001192826.1
Danio rerio (zebrafish)	ubr1	XP_002665264.3
Xenopus tropicalis (tropical clawed frog)	ubr1	XP_002941132.2
Rattus norvegicus (Norway rat)	Ubr1	NP_001171543.1
Homo sapiens (human)	UBR1	NP_777576.1
Mus musculus (house mouse)	Ubr1	NP_033487.2
Gallus gallus (chicken)	UBR1	XP_421165.3
Schizosaccharomyces pombe (fission yeast)	ubr1	NP_596158.2
Magnaporthe oryzae (rice blast fungus)	MGG_13171	XP_003710667.1
Neurospora crassa	NCU03234	XP_965334.2

Gene Ontology
Bibliography

Related articles in PubMed

Expanding the mutational spectrum in Johanson-Blizzard syndrome: identification of whole exon deletions and duplications in the UBR1 gene by multiplex ligation-dependent probe amplification analysis.
Sukalo M, Sch?flein E, Schanze I, Everman DB, Rezaei N, Argente J, Lorda-Sanchez I, Deshpande C, Takahashi T, Kleger A, Zenker M
Molecular genetics & genomic medicine5(6)774-780(2017 Nov)

Variants in the UBR1 gene are not associated with chronic pancreatitis in Japan.
Masamune A, Nakano E, Niihori T, Hamada S, Nagasaki M, Aoki Y, Shimosegawa T
Pancreatology : official journal of the International Association of Pancreatology (IAP) ... [et al.]16(5)814-8(2016 Sep-Oct)

Implication of a Chromosome 15q15.2 Locus in Regulating UBR1 and Predisposing Smokers to MGMT Methylation in Lung.
Leng S, Wu G, Collins LB, Thomas CL, Tellez CS, Jauregui AR, Picchi MA, Zhang X, Juri DE, Desai D, Amin SG, Crowell RE, Stidley CA, Liu Y, Swenberg JA, Lin Y, Wathelet MG, Gilliland FD, Belinsky SA
Cancer research75(15)3108-17(2015 Aug)

Mutations in the human UBR1 gene and the associated phenotypic spectrum.
Sukalo M, Fiedler A, Guzm?n C, Spranger S, Addor MC, McHeik JN, Oltra Benavent M, Cobben JM, Gillis LA, Shealy AG, Deshpande C, Bozorgmehr B, Everman DB, Stattin EL, Liebelt J, Keller KM, Bertola DR, van Karnebeek CD, Bergmann C, Liu Z, D?ker G, Rezaei N, Alkuraya FS, O?ur G, Alrajoudi A, Venegas-Vega CA, Verbeek NE, Richmond EJ, Kirbiyik O, Ranganath P, Singh A, Godbole K, Ali FA, Alves C, Mayerle J, Lerch MM, Witt H, Zenker M
Human mutation35(5)521-31(2014 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

UBR1 mutations of single or multi-exon deletions or duplications account for a substantial proportion of Johanson-Blizzard syndrome.
Title: Expanding the mutational spectrum in Johanson-Blizzard syndrome: identification of whole exon deletions and duplications in the UBR1 gene by multiplex ligation-dependent probe amplification analysis.

The frequency of any non-synonymous or synonymous variants was not different between the patients with chronic pancreatitis and controls
Title: Variants in the UBR1 gene are not associated with chronic pancreatitis in Japan.

Reduced UBR1 expression affects MGMT turnover and DNA repair in the smokers lungs.
Title: Implication of a Chromosome 15q15.2 Locus in Regulating UBR1 and Predisposing Smokers to MGMT Methylation in Lung.

For all previously reported and novel UBR1 mutations together with their clinical data, a mutation database has been established at LOVD.
Title: Mutations in the human UBR1 gene and the associated phenotypic spectrum.

Results confirmed the relevance of specific missense UBR1 alleles to JBS, and suggested that a residual activity of a missense allele is causally associated with milder variants of JBS.
Title: Ubiquitin ligases of the N-end rule pathway: assessment of mutations in UBR1 that cause the Johanson-Blizzard syndrome.

The following UBR1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the UBR1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu19665	NM_174916.3 Latest version!	Homo sapiens ubiquitin protein ligase E3 component n-recognin 1 (UBR1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	22	$923.30 $1319.00
View Old Accession Versions >>
OHu19665	NM_174916.2	Homo sapiens ubiquitin protein ligase E3 component n-recognin 1 (UBR1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	22	$923.30 $1319.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

UBR1 ( NM_174916.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_777576.1 Homo sapiens ubiquitin protein ligase E3 component n-recognin 1 (UBR1), mRNA.

Price & Availability↑

CloneID	OHu19665
Clone ID Related Accession (Same CDS sequence)	NM_174916.2 , NM_174916.3
Accession Version	NM_174916.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5250bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase UBR1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BE873236.1, AY061886.1, AK127478.1 and BX648400.1. This sequence is a reference standard in the RefSeqGene project. On Dec 15, 2005 this sequence version replaced NM_174916.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.254533.1, SRR1803613.36884.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGACG AGGAGGCTGG AGGTACTGAG AGGATGGAAA TCAGCGCGGA GTTACCCCAG 
ACCCCTCAGC GTCTGGCATC TTGGTGGGAT CAGCAAGTTG ATTTTTATAC TGCTTTCTTG 
CATCATTTGG CACAATTGGT GCCAGAAATT TACTTTGCTG AAATGGACCC AGACTTGGAA 
AAGCAGGAGG AAAGTGTACA AATGTCAATA TTCACTCCAC TGGAATGGTA CTTATTTGGA 
GAAGATCCAG ATATTTGCTT AGAGAAATTG AAGCACAGTG GAGCATTTCA GCTTTGTGGG 
AGGGTTTTCA AAAGTGGAGA GACAACCTAT TCTTGCAGGG ATTGTGCAAT TGATCCAACA 
TGTGTACTCT GTATGGACTG CTTCCAGGAC AGTGTTCATA AAAATCATCG TTACAAGATG 
CATACTTCTA CTGGAGGAGG GTTCTGTGAC TGTGGAGACA CAGAGGCATG GAAAACTGGC 
CCTTTTTGTG TAAATCATGA ACCTGGAAGA GCAGGTACTA TAAAAGAGAA TTCACGCTGT 
CCGTTGAATG AAGAGGTAAT TGTCCAAGCC AGGAAAATAT TTCCTTCAGT GATAAAATAT 
GTCGTAGAAA TGACTATATG GGAAGAGGAA AAAGAACTGC CTCCTGAACT CCAGATAAGG 
GAGAAAAATG AAAGATACTA TTGTGTCCTT TTCAATGATG AACACCATTC ATATGACCAC 
GTCATATACA GCCTACAAAG AGCTCTTGAC TGTGAGCTCG CAGAGGCCCA GTTGCATACC 
ACTGCCATTG ACAAAGAGGG TCGTCGGGCT GTTAAAGCGG GAGCTTATGC TGCTTGCCAG 
GAAGCAAAGG AAGATATAAA GAGTCATTCA GAAAATGTCT CTCAACATCC ACTTCATGTA 
GAAGTATTAC ACTCAGAGAT TATGGCTCAT CAGAAATTTG CTTTGCGTCT TGGTTCCTGG 
ATGAACAAAA TTATGAGCTA TTCAAGTGAC TTTAGGCAGA TCTTTTGCCA AGCATGCCTT 
AGAGAAGAAC CTGACTCGGA GAATCCCTGT CTCATAAGCA GGTTAATGCT TTGGGATGCA 
AAGCTTTATA AAGGTGCCCG TAAGATCCTT CATGAATTGA TCTTCAGCAG TTTTTTTATG 
GAGATGGAAT ACAAAAAACT CTTTGCTATG GAATTTGTGA AGTATTATAA ACAACTGCAG 
AAAGAATATA TCAGTGATGA TCATGACAGA AGTATCTCTA TAACTGCACT TTCAGTTCAG 
ATGTTTACTG TTCCTACTCT GGCTCGACAT CTTATTGAAG AGCAGAATGT TATCTCTGTC 
ATTACTGAAA CTCTGCTAGA AGTTTTACCT GAGTACTTGG ACAGGAACAA TAAATTCAAC 
TTCCAGGGTT ATAGCCAGGA CAAATTGGGA AGAGTATATG CAGTAATATG TGACCTAAAG 
TATATCCTGA TCAGCAAACC CACAATATGG ACAGAAAGAT TAAGAATGCA GTTCCTTGAA 
GGTTTTCGAT CTTTTTTGAA GATTCTTACC TGTATGCAGG GAATGGAAGA AATCCGAAGA 
CAGGTTGGGC AACACATTGA AGTGGATCCT GATTGGGAGG CTGCCATTGC TATACAGATG 
CAATTGAAGA ATATTTTACT CATGTTCCAA GAGTGGTGTG CTTGTGATGA AGAACTCTTA 
CTTGTGGCTT ATAAAGAATG TCACAAAGCT GTGATGAGGT GCAGTACCAG TTTCATATCT 
AGTAGCAAGA CAGTAGTACA ATCGTGTGGA CATAGTTTGG AAACAAAGTC CTACAGAGTA 
TCTGAGGATC TTGTAAGCAT ACATCTGCCA CTCTCTAGGA CCCTTGCTGG TCTTCATGTA 
CGTTTAAGCA GGCTGGGTGC TGTTTCAAGA CTGCATGAAT TTGTGTCTTT TGAGGACTTT 
CAAGTAGAGG TACTAGTGGA ATATCCTTTA CGTTGTCTGG TGTTGGTTGC CCAGGTTGTT 
GCTGAGATGT GGCGAAGAAA TGGACTGTCT CTTATTAGCC AGGTGTTTTA TTACCAAGAT 
GTTAAGTGCA GAGAAGAAAT GTATGATAAA GATATCATCA TGCTTCAGAT TGGTGCATCT 
TTAATGGATC CCAATAAGTT CTTGTTACTG GTACTTCAGA GGTATGAACT TGCCGAGGCT 
TTTAACAAGA CCATATCTAC AAAAGACCAG GATTTGATTA AACAATATAA TACACTAATA 
GAAGAAATGC TTCAGGTCCT CATCTATATT GTGGGTGAGC GTTATGTACC TGGAGTGGGA 
AATGTGACCA AAGAAGAGGT CACAATGAGA GAAATCATTC ACTTGCTTTG CATTGAACCC 
ATGCCACACA GTGCCATTGC CAAAAATTTA CCTGAGAATG AAAATAATGA AACTGGCTTA 
GAGAATGTCA TAAACAAAGT GGCCACATTT AAGAAACCAG GTGTATCAGG CCATGGAGTT 
TATGAACTAA AAGATGAATC ACTGAAAGAC TTCAATATGT ACTTTTATCA TTACTCCAAA 
ACCCAGCATA GCAAGGCTGA ACATATGCAG AAGAAAAGGA GAAAACAAGA AAACAAAGAT 
GAAGCATTGC CGCCACCACC ACCTCCTGAA TTCTGCCCTG CTTTCAGCAA AGTGATTAAC 
CTTCTCAACT GTGATATCAT GATGTACATT CTCAGGACCG TATTTGAGCG GGCAATAGAC 
ACAGATTCTA ACTTGTGGAC CGAAGGGATG CTCCAAATGG CTTTTCATAT TCTGGCATTG 
GGTTTACTAG AAGAGAAGCA ACAGCTTCAA AAAGCTCCTG AAGAAGAAGT AACATTTGAC 
TTTTATCATA AGGCTTCAAG ATTGGGAAGT TCAGCCATGA ATATACAAAT GCTTTTGGAA 
AAACTCAAAG GAATTCCCCA GTTAGAAGGC CAGAAGGACA TGATAACGTG GATACTTCAG 
ATGTTTGACA CAGTGAAGCG ATTAAGAGAA AAATCTTGTT TAATTGTAGC AACCACATCA 
GGATCGGAAT CTATTAAGAA TGATGAGATT ACTCATGATA AAGAAAAAGC AGAACGAAAA 
AGAAAAGCTG AAGCTGCTAG GCTACATCGC CAGAAGATCA TGGCTCAGAT GTCTGCCTTA 
CAGAAAAACT TCATTGAAAC TCATAAACTC ATGTATGACA ATACATCAGA AATGCCTGGG 
AAAGAAGATT CCATTATGGA GGAAGAGAGC ACCCCAGCAG TCAGTGACTA CTCTAGAATT 
GCTTTGGGTC CTAAACGGGG TCCATCTGTT ACTGAAAAGG AGGTGCTGAC GTGCATCCTT 
TGCCAAGAAG AACAGGAGGT GAAAATAGAA AATAATGCCA TGGTATTATC GGCCTGTGTC 
CAGAAATCTA CTGCCTTAAC CCAGCACAGG GGAAAACCCA TAGAACTCTC AGGAGAAGCC 
CTAGACCCAC TTTTCATGGA TCCAGACTTG GCATATGGAA CTTATACAGG AAGCTGTGGT 
CATGTAATGC ACGCAGTGTG CTGGCAGAAG TATTTTGAAG CTGTACAGCT GAGCTCTCAG 
CAGCGCATTC ATGTTGACCT TTTTGACTTG GAAAGTGGAG AATATCTTTG CCCTCTTTGC 
AAATCTCTGT GCAATACTGT GATCCCCATT ATTCCTTTGC AACCTCAAAA GATAAACAGT 
GAGAATGCAG ATGCTCTTGC TCAACTTTTG ACCCTGGCAC GGTGGATACA GACTGTTCTG 
GCCAGAATAT CAGGTTATAA TATAAGACAT GCTAAAGGAG AAAACCCAAT TCCTATTTTC 
TTTAATCAAG GAATGGGAGA TTCTACTTTG GAGTTCCATT CCATCCTGAG TTTTGGCGTT 
GAGTCTTCGA TTAAATATTC AAATAGCATC AAGGAAATGG TTATTCTCTT TGCCACAACA 
ATTTATAGAA TTGGATTGAA AGTGCCACCT GATGAAAGGG ATCCTCGAGT CCCCATGCTG 
ACCTGGAGCA CCTGCGCTTT CACTATCCAG GCAATTGAAA ATCTATTGGG AGATGAAGGA 
AAACCTCTGT TTGGAGCACT TCAAAATAGG CAGCATAATG GTCTGAAAGC ATTAATGCAG 
TTTGCAGTTG CACAGAGGAT TACCTGTCCT CAGGTCCTGA TACAGAAACA TCTGGTTCGT 
CTTCTATCAG TTGTTCTTCC TAACATAAAA TCAGAAGATA CACCATGCCT TCTGTCTATA 
GATCTGTTTC ATGTTTTGGT GGGTGCTGTG TTAGCATTCC CATCCTTGTA TTGGGATGAC 
CCTGTTGATC TGCAGCCTTC TTCAGTTAGT TCTTCCTATA ACCACCTTTA TCTCTTCCAT 
TTGATCACCA TGGCACACAT GCTTCAGATA CTACTTACAG TAGACACAGG CCTACCCCTT 
GCTCAGGTTC AAGAAGACAG TGAAGAGGCT CATTCCGCAT CTTCTTTCTT TGCAGAAATT 
TCTCAATATA CAAGTGGCTC CATTGGGTGT GATATTCCTG GCTGGTATTT GTGGGTCTCA 
CTGAAGAATG GCATCACCCC TTATCTTCGC TGTGCTGCAT TGTTTTTCCA CTATTTACTT 
GGGGTAACTC CGCCTGAGGA ACTGCATACC AATTCTGCAG AAGGAGAGTA CAGTGCACTC 
TGTAGCTATC TATCTTTACC TACAAATTTG TTCCTGCTCT TCCAGGAATA TTGGGATACT 
GTAAGGCCCT TGCTCCAGAG GTGGTGTGCA GATCCTGCCT TACTAAACTG TTTGAAGCAA 
AAAAACACCG TGGTCAGGTA CCCTAGAAAA AGAAATAGTT TGATAGAGCT TCCTGATGAC 
TATAGCTGCC TCCTGAATCA AGCTTCTCAT TTCAGGTGCC CACGGTCTGC AGATGATGAG 
CGAAAGCATC CTGTCCTCTG CCTTTTCTGT GGGGCTATAC TATGTTCTCA GAACATTTGC 
TGCCAGGAAA TTGTGAACGG GGAAGAGGTT GGAGCTTGCA TTTTTCACGC ACTTCACTGT 
GGAGCCGGAG TCTGCATTTT CCTAAAAATC AGAGAATGCC GAGTGGTCCT GGTTGAAGGT 
AAAGCCAGAG GCTGTGCCTA TCCAGCTCCT TACTTGGATG AATATGGAGA AACAGACCCT 
GGCCTGAAGA GGGGCAACCC CCTTCATTTA TCTCGTGAGC GGTATCGGAA GCTCCATTTG 
GTCTGGCAAC AACACTGCAT TATAGAAGAG ATTGCTAGGA GCCAAGAGAC TAATCAGATG 
TTATTTGGAT TCAACTGGCA GTTACTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_777576.1
CDS	67..5316
Translation	MADEEAGGTERMEISAELPQTPQRLASWWDQQVDFYTAFLHHLAQLVPEIYFAEMDPDLEKQEESVQMSIFTPLEWYLFGEDPDICLEKLKHSGAFQLCGRVFKSGETTYSCRDCAIDPTCVLCMDCFQDSVHKNHRYKMHTSTGGGFCDCGDTEAWKTGPFCVNHEPGRAGTIKENSRCPLNEEVIVQARKIFPSVIKYVVEMTIWEEEKELPPELQIREKNERYYCVLFNDEHHSYDHVIYSLQRALDCELAEAQLHTTAIDKEGRRAVKAGAYAACQEAKEDIKSHSENVSQHPLHVEVLHSEIMAHQKFALRLGSWMNKIMSYSSDFRQIFCQACLREEPDSENPCLISRLMLWDAKLYKGARKILHELIFSSFFMEMEYKKLFAMEFVKYYKQLQKEYISDDHDRSISITALSVQMFTVPTLARHLIEEQNVISVITETLLEVLPEYLDRNNKFNFQGYSQDKLGRVYAVICDLKYILISKPTIWTERLRMQFLEGFRSFLKILTCMQGMEEIRRQVGQHIEVDPDWEAAIAIQMQLKNILLMFQEWCACDEELLLVAYKECHKAVMRCSTSFISSSKTVVQSCGHSLETKSYRVSEDLVSIHLPLSRTLAGLHVRLSRLGAVSRLHEFVSFEDFQVEVLVEYPLRCLVLVAQVVAEMWRRNGLSLISQVFYYQDVKCREEMYDKDIIMLQIGASLMDPNKFLLLVLQRYELAEAFNKTISTKDQDLIKQYNTLIEEMLQVLIYIVGERYVPGVGNVTKEEVTMREIIHLLCIEPMPHSAIAKNLPENENNETGLENVINKVATFKKPGVSGHGVYELKDESLKDFNMYFYHYSKTQHSKAEHMQKKRRKQENKDEALPPPPPPEFCPAFSKVINLLNCDIMMYILRTVFERAIDTDSNLWTEGMLQMAFHILALGLLEEKQQLQKAPEEEVTFDFYHKASRLGSSAMNIQMLLEKLKGIPQLEGQKDMITWILQMFDTVKRLREKSCLIVATTSGSESIKNDEITHDKEKAERKRKAEAARLHRQKIMAQMSALQKNFIETHKLMYDNTSEMPGKEDSIMEEESTPAVSDYSRIALGPKRGPSVTEKEVLTCILCQEEQEVKIENNAMVLSACVQKSTALTQHRGKPIELSGEALDPLFMDPDLAYGTYTGSCGHVMHAVCWQKYFEAVQLSSQQRIHVDLFDLESGEYLCPLCKSLCNTVIPIIPLQPQKINSENADALAQLLTLARWIQTVLARISGYNIRHAKGENPIPIFFNQGMGDSTLEFHSILSFGVESSIKYSNSIKEMVILFATTIYRIGLKVPPDERDPRVPMLTWSTCAFTIQAIENLLGDEGKPLFGALQNRQHNGLKALMQFAVAQRITCPQVLIQKHLVRLLSVVLPNIKSEDTPCLLSIDLFHVLVGAVLAFPSLYWDDPVDLQPSSVSSSYNHLYLFHLITMAHMLQILLTVDTGLPLAQVQEDSEEAHSASSFFAEISQYTSGSIGCDIPGWYLWVSLKNGITPYLRCAALFFHYLLGVTPPEELHTNSAEGEYSALCSYLSLPTNLFLLFQEYWDTVRPLLQRWCADPALLNCLKQKNTVVRYPRKRNSLIELPDDYSCLLNQASHFRCPRSADDERKHPVLCLFCGAILCSQNICCQEIVNGEEVGACIFHALHCGAGVCIFLKIRECRVVLVEGKARGCAYPAPYLDEYGETDPGLKRGNPLHLSRERYRKLHLVWQQHCIIEEIARSQETNQMLFGFNWQLL

Target ORF information:

RefSeq Version	NM_174916.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin protein ligase E3 component n-recognin 1 (UBR1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_174916.2

ORF Insert Sequence:

ATGGCGGACG AGGAGGCTGG AGGTACTGAG AGGATGGAAA TCAGCGCGGA GTTACCCCAG 
ACCCCTCAGC GTCTGGCATC TTGGTGGGAT CAGCAAGTTG ATTTTTATAC TGCTTTCTTG 
CATCATTTGG CACAATTGGT GCCAGAAATT TACTTTGCTG AAATGGACCC AGACTTGGAA 
AAGCAGGAGG AAAGTGTACA AATGTCAATA TTCACTCCAC TGGAATGGTA CTTATTTGGA 
GAAGATCCAG ATATTTGCTT AGAGAAATTG AAGCACAGTG GAGCATTTCA GCTTTGTGGG 
AGGGTTTTCA AAAGTGGAGA GACAACCTAT TCTTGCAGGG ATTGTGCAAT TGATCCAACA 
TGTGTACTCT GTATGGACTG CTTCCAGGAC AGTGTTCATA AAAATCATCG TTACAAGATG 
CATACTTCTA CTGGAGGAGG GTTCTGTGAC TGTGGAGACA CAGAGGCATG GAAAACTGGC 
CCTTTTTGTG TAAATCATGA ACCTGGAAGA GCAGGTACTA TAAAAGAGAA TTCACGCTGT 
CCGTTGAATG AAGAGGTAAT TGTCCAAGCC AGGAAAATAT TTCCTTCAGT GATAAAATAT 
GTCGTAGAAA TGACTATATG GGAAGAGGAA AAAGAACTGC CTCCTGAACT CCAGATAAGG 
GAGAAAAATG AAAGATACTA TTGTGTCCTT TTCAATGATG AACACCATTC ATATGACCAC 
GTCATATACA GCCTACAAAG AGCTCTTGAC TGTGAGCTCG CAGAGGCCCA GTTGCATACC 
ACTGCCATTG ACAAAGAGGG TCGTCGGGCT GTTAAAGCGG GAGCTTATGC TGCTTGCCAG 
GAAGCAAAGG AAGATATAAA GAGTCATTCA GAAAATGTCT CTCAACATCC ACTTCATGTA 
GAAGTATTAC ACTCAGAGAT TATGGCTCAT CAGAAATTTG CTTTGCGTCT TGGTTCCTGG 
ATGAACAAAA TTATGAGCTA TTCAAGTGAC TTTAGGCAGA TCTTTTGCCA AGCATGCCTT 
AGAGAAGAAC CTGACTCGGA GAATCCCTGT CTCATAAGCA GGTTAATGCT TTGGGATGCA 
AAGCTTTATA AAGGTGCCCG TAAGATCCTT CATGAATTGA TCTTCAGCAG TTTTTTTATG 
GAGATGGAAT ACAAAAAACT CTTTGCTATG GAATTTGTGA AGTATTATAA ACAACTGCAG 
AAAGAATATA TCAGTGATGA TCATGACAGA AGTATCTCTA TAACTGCACT TTCAGTTCAG 
ATGTTTACTG TTCCTACTCT GGCTCGACAT CTTATTGAAG AGCAGAATGT TATCTCTGTC 
ATTACTGAAA CTCTGCTAGA AGTTTTACCT GAGTACTTGG ACAGGAACAA TAAATTCAAC 
TTCCAGGGTT ATAGCCAGGA CAAATTGGGA AGAGTATATG CAGTAATATG TGACCTAAAG 
TATATCCTGA TCAGCAAACC CACAATATGG ACAGAAAGAT TAAGAATGCA GTTCCTTGAA 
GGTTTTCGAT CTTTTTTGAA GATTCTTACC TGTATGCAGG GAATGGAAGA AATCCGAAGA 
CAGGTTGGGC AACACATTGA AGTGGATCCT GATTGGGAGG CTGCCATTGC TATACAGATG 
CAATTGAAGA ATATTTTACT CATGTTCCAA GAGTGGTGTG CTTGTGATGA AGAACTCTTA 
CTTGTGGCTT ATAAAGAATG TCACAAAGCT GTGATGAGGT GCAGTACCAG TTTCATATCT 
AGTAGCAAGA CAGTAGTACA ATCGTGTGGA CATAGTTTGG AAACAAAGTC CTACAGAGTA 
TCTGAGGATC TTGTAAGCAT ACATCTGCCA CTCTCTAGGA CCCTTGCTGG TCTTCATGTA 
CGTTTAAGCA GGCTGGGTGC TGTTTCAAGA CTGCATGAAT TTGTGTCTTT TGAGGACTTT 
CAAGTAGAGG TACTAGTGGA ATATCCTTTA CGTTGTCTGG TGTTGGTTGC CCAGGTTGTT 
GCTGAGATGT GGCGAAGAAA TGGACTGTCT CTTATTAGCC AGGTGTTTTA TTACCAAGAT 
GTTAAGTGCA GAGAAGAAAT GTATGATAAA GATATCATCA TGCTTCAGAT TGGTGCATCT 
TTAATGGATC CCAATAAGTT CTTGTTACTG GTACTTCAGA GGTATGAACT TGCCGAGGCT 
TTTAACAAGA CCATATCTAC AAAAGACCAG GATTTGATTA AACAATATAA TACACTAATA 
GAAGAAATGC TTCAGGTCCT CATCTATATT GTGGGTGAGC GTTATGTACC TGGAGTGGGA 
AATGTGACCA AAGAAGAGGT CACAATGAGA GAAATCATTC ACTTGCTTTG CATTGAACCC 
ATGCCACACA GTGCCATTGC CAAAAATTTA CCTGAGAATG AAAATAATGA AACTGGCTTA 
GAGAATGTCA TAAACAAAGT GGCCACATTT AAGAAACCAG GTGTATCAGG CCATGGAGTT 
TATGAACTAA AAGATGAATC ACTGAAAGAC TTCAATATGT ACTTTTATCA TTACTCCAAA 
ACCCAGCATA GCAAGGCTGA ACATATGCAG AAGAAAAGGA GAAAACAAGA AAACAAAGAT 
GAAGCATTGC CGCCACCACC ACCTCCTGAA TTCTGCCCTG CTTTCAGCAA AGTGATTAAC 
CTTCTCAACT GTGATATCAT GATGTACATT CTCAGGACCG TATTTGAGCG GGCAATAGAC 
ACAGATTCTA ACTTGTGGAC CGAAGGGATG CTCCAAATGG CTTTTCATAT TCTGGCATTG 
GGTTTACTAG AAGAGAAGCA ACAGCTTCAA AAAGCTCCTG AAGAAGAAGT AACATTTGAC 
TTTTATCATA AGGCTTCAAG ATTGGGAAGT TCAGCCATGA ATATACAAAT GCTTTTGGAA 
AAACTCAAAG GAATTCCCCA GTTAGAAGGC CAGAAGGACA TGATAACGTG GATACTTCAG 
ATGTTTGACA CAGTGAAGCG ATTAAGAGAA AAATCTTGTT TAATTGTAGC AACCACATCA 
GGATCGGAAT CTATTAAGAA TGATGAGATT ACTCATGATA AAGAAAAAGC AGAACGAAAA 
AGAAAAGCTG AAGCTGCTAG GCTACATCGC CAGAAGATCA TGGCTCAGAT GTCTGCCTTA 
CAGAAAAACT TCATTGAAAC TCATAAACTC ATGTATGACA ATACATCAGA AATGCCTGGG 
AAAGAAGATT CCATTATGGA GGAAGAGAGC ACCCCAGCAG TCAGTGACTA CTCTAGAATT 
GCTTTGGGTC CTAAACGGGG TCCATCTGTT ACTGAAAAGG AGGTGCTGAC GTGCATCCTT 
TGCCAAGAAG AACAGGAGGT GAAAATAGAA AATAATGCCA TGGTATTATC GGCCTGTGTC 
CAGAAATCTA CTGCCTTAAC CCAGCACAGG GGAAAACCCA TAGAACTCTC AGGAGAAGCC 
CTAGACCCAC TTTTCATGGA TCCAGACTTG GCATATGGAA CTTATACAGG AAGCTGTGGT 
CATGTAATGC ACGCAGTGTG CTGGCAGAAG TATTTTGAAG CTGTACAGCT GAGCTCTCAG 
CAGCGCATTC ATGTTGACCT TTTTGACTTG GAAAGTGGAG AATATCTTTG CCCTCTTTGC 
AAATCTCTGT GCAATACTGT GATCCCCATT ATTCCTTTGC AACCTCAAAA GATAAACAGT 
GAGAATGCAG ATGCTCTTGC TCAACTTTTG ACCCTGGCAC GGTGGATACA GACTGTTCTG 
GCCAGAATAT CAGGTTATAA TATAAGACAT GCTAAAGGAG AAAACCCAAT TCCTATTTTC 
TTTAATCAAG GAATGGGAGA TTCTACTTTG GAGTTCCATT CCATCCTGAG TTTTGGCGTT 
GAGTCTTCGA TTAAATATTC AAATAGCATC AAGGAAATGG TTATTCTCTT TGCCACAACA 
ATTTATAGAA TTGGATTGAA AGTGCCACCT GATGAAAGGG ATCCTCGAGT CCCCATGCTG 
ACCTGGAGCA CCTGCGCTTT CACTATCCAG GCAATTGAAA ATCTATTGGG AGATGAAGGA 
AAACCTCTGT TTGGAGCACT TCAAAATAGG CAGCATAATG GTCTGAAAGC ATTAATGCAG 
TTTGCAGTTG CACAGAGGAT TACCTGTCCT CAGGTCCTGA TACAGAAACA TCTGGTTCGT 
CTTCTATCAG TTGTTCTTCC TAACATAAAA TCAGAAGATA CACCATGCCT TCTGTCTATA 
GATCTGTTTC ATGTTTTGGT GGGTGCTGTG TTAGCATTCC CATCCTTGTA TTGGGATGAC 
CCTGTTGATC TGCAGCCTTC TTCAGTTAGT TCTTCCTATA ACCACCTTTA TCTCTTCCAT 
TTGATCACCA TGGCACACAT GCTTCAGATA CTACTTACAG TAGACACAGG CCTACCCCTT 
GCTCAGGTTC AAGAAGACAG TGAAGAGGCT CATTCCGCAT CTTCTTTCTT TGCAGAAATT 
TCTCAATATA CAAGTGGCTC CATTGGGTGT GATATTCCTG GCTGGTATTT GTGGGTCTCA 
CTGAAGAATG GCATCACCCC TTATCTTCGC TGTGCTGCAT TGTTTTTCCA CTATTTACTT 
GGGGTAACTC CGCCTGAGGA ACTGCATACC AATTCTGCAG AAGGAGAGTA CAGTGCACTC 
TGTAGCTATC TATCTTTACC TACAAATTTG TTCCTGCTCT TCCAGGAATA TTGGGATACT 
GTAAGGCCCT TGCTCCAGAG GTGGTGTGCA GATCCTGCCT TACTAAACTG TTTGAAGCAA 
AAAAACACCG TGGTCAGGTA CCCTAGAAAA AGAAATAGTT TGATAGAGCT TCCTGATGAC 
TATAGCTGCC TCCTGAATCA AGCTTCTCAT TTCAGGTGCC CACGGTCTGC AGATGATGAG 
CGAAAGCATC CTGTCCTCTG CCTTTTCTGT GGGGCTATAC TATGTTCTCA GAACATTTGC 
TGCCAGGAAA TTGTGAACGG GGAAGAGGTT GGAGCTTGCA TTTTTCACGC ACTTCACTGT 
GGAGCCGGAG TCTGCATTTT CCTAAAAATC AGAGAATGCC GAGTGGTCCT GGTTGAAGGT 
AAAGCCAGAG GCTGTGCCTA TCCAGCTCCT TACTTGGATG AATATGGAGA AACAGACCCT 
GGCCTGAAGA GGGGCAACCC CCTTCATTTA TCTCGTGAGC GGTATCGGAA GCTCCATTTG 
GTCTGGCAAC AACACTGCAT TATAGAAGAG ATTGCTAGGA GCCAAGAGAC TAATCAGATG 
TTATTTGGAT TCAACTGGCA GTTACTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UBR1 ( NM_174916.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_777576.1 Homo sapiens ubiquitin protein ligase E3 component n-recognin 1 (UBR1), mRNA.

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CloneID	OHu19665
Clone ID Related Accession (Same CDS sequence)	NM_174916.2 , NM_174916.3
Accession Version	NM_174916.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5250bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-23
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase UBR1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BE873236.1, AY061886.1, AK127478.1 and BX648400.1. This sequence is a reference standard in the RefSeqGene project. On Nov 23, 2018 this sequence version replaced NM_174916.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.36884.1, SRR1803616.254533.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000290650.9/ ENSP00000290650.4 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCGGACG AGGAGGCTGG AGGTACTGAG AGGATGGAAA TCAGCGCGGA GTTACCCCAG 
ACCCCTCAGC GTCTGGCATC TTGGTGGGAT CAGCAAGTTG ATTTTTATAC TGCTTTCTTG 
CATCATTTGG CACAATTGGT GCCAGAAATT TACTTTGCTG AAATGGACCC AGACTTGGAA 
AAGCAGGAGG AAAGTGTACA AATGTCAATA TTCACTCCAC TGGAATGGTA CTTATTTGGA 
GAAGATCCAG ATATTTGCTT AGAGAAATTG AAGCACAGTG GAGCATTTCA GCTTTGTGGG 
AGGGTTTTCA AAAGTGGAGA GACAACCTAT TCTTGCAGGG ATTGTGCAAT TGATCCAACA 
TGTGTACTCT GTATGGACTG CTTCCAGGAC AGTGTTCATA AAAATCATCG TTACAAGATG 
CATACTTCTA CTGGAGGAGG GTTCTGTGAC TGTGGAGACA CAGAGGCATG GAAAACTGGC 
CCTTTTTGTG TAAATCATGA ACCTGGAAGA GCAGGTACTA TAAAAGAGAA TTCACGCTGT 
CCGTTGAATG AAGAGGTAAT TGTCCAAGCC AGGAAAATAT TTCCTTCAGT GATAAAATAT 
GTCGTAGAAA TGACTATATG GGAAGAGGAA AAAGAACTGC CTCCTGAACT CCAGATAAGG 
GAGAAAAATG AAAGATACTA TTGTGTCCTT TTCAATGATG AACACCATTC ATATGACCAC 
GTCATATACA GCCTACAAAG AGCTCTTGAC TGTGAGCTCG CAGAGGCCCA GTTGCATACC 
ACTGCCATTG ACAAAGAGGG TCGTCGGGCT GTTAAAGCGG GAGCTTATGC TGCTTGCCAG 
GAAGCAAAGG AAGATATAAA GAGTCATTCA GAAAATGTCT CTCAACATCC ACTTCATGTA 
GAAGTATTAC ACTCAGAGAT TATGGCTCAT CAGAAATTTG CTTTGCGTCT TGGTTCCTGG 
ATGAACAAAA TTATGAGCTA TTCAAGTGAC TTTAGGCAGA TCTTTTGCCA AGCATGCCTT 
AGAGAAGAAC CTGACTCGGA GAATCCCTGT CTCATAAGCA GGTTAATGCT TTGGGATGCA 
AAGCTTTATA AAGGTGCCCG TAAGATCCTT CATGAATTGA TCTTCAGCAG TTTTTTTATG 
GAGATGGAAT ACAAAAAACT CTTTGCTATG GAATTTGTGA AGTATTATAA ACAACTGCAG 
AAAGAATATA TCAGTGATGA TCATGACAGA AGTATCTCTA TAACTGCACT TTCAGTTCAG 
ATGTTTACTG TTCCTACTCT GGCTCGACAT CTTATTGAAG AGCAGAATGT TATCTCTGTC 
ATTACTGAAA CTCTGCTAGA AGTTTTACCT GAGTACTTGG ACAGGAACAA TAAATTCAAC 
TTCCAGGGTT ATAGCCAGGA CAAATTGGGA AGAGTATATG CAGTAATATG TGACCTAAAG 
TATATCCTGA TCAGCAAACC CACAATATGG ACAGAAAGAT TAAGAATGCA GTTCCTTGAA 
GGTTTTCGAT CTTTTTTGAA GATTCTTACC TGTATGCAGG GAATGGAAGA AATCCGAAGA 
CAGGTTGGGC AACACATTGA AGTGGATCCT GATTGGGAGG CTGCCATTGC TATACAGATG 
CAATTGAAGA ATATTTTACT CATGTTCCAA GAGTGGTGTG CTTGTGATGA AGAACTCTTA 
CTTGTGGCTT ATAAAGAATG TCACAAAGCT GTGATGAGGT GCAGTACCAG TTTCATATCT 
AGTAGCAAGA CAGTAGTACA ATCGTGTGGA CATAGTTTGG AAACAAAGTC CTACAGAGTA 
TCTGAGGATC TTGTAAGCAT ACATCTGCCA CTCTCTAGGA CCCTTGCTGG TCTTCATGTA 
CGTTTAAGCA GGCTGGGTGC TGTTTCAAGA CTGCATGAAT TTGTGTCTTT TGAGGACTTT 
CAAGTAGAGG TACTAGTGGA ATATCCTTTA CGTTGTCTGG TGTTGGTTGC CCAGGTTGTT 
GCTGAGATGT GGCGAAGAAA TGGACTGTCT CTTATTAGCC AGGTGTTTTA TTACCAAGAT 
GTTAAGTGCA GAGAAGAAAT GTATGATAAA GATATCATCA TGCTTCAGAT TGGTGCATCT 
TTAATGGATC CCAATAAGTT CTTGTTACTG GTACTTCAGA GGTATGAACT TGCCGAGGCT 
TTTAACAAGA CCATATCTAC AAAAGACCAG GATTTGATTA AACAATATAA TACACTAATA 
GAAGAAATGC TTCAGGTCCT CATCTATATT GTGGGTGAGC GTTATGTACC TGGAGTGGGA 
AATGTGACCA AAGAAGAGGT CACAATGAGA GAAATCATTC ACTTGCTTTG CATTGAACCC 
ATGCCACACA GTGCCATTGC CAAAAATTTA CCTGAGAATG AAAATAATGA AACTGGCTTA 
GAGAATGTCA TAAACAAAGT GGCCACATTT AAGAAACCAG GTGTATCAGG CCATGGAGTT 
TATGAACTAA AAGATGAATC ACTGAAAGAC TTCAATATGT ACTTTTATCA TTACTCCAAA 
ACCCAGCATA GCAAGGCTGA ACATATGCAG AAGAAAAGGA GAAAACAAGA AAACAAAGAT 
GAAGCATTGC CGCCACCACC ACCTCCTGAA TTCTGCCCTG CTTTCAGCAA AGTGATTAAC 
CTTCTCAACT GTGATATCAT GATGTACATT CTCAGGACCG TATTTGAGCG GGCAATAGAC 
ACAGATTCTA ACTTGTGGAC CGAAGGGATG CTCCAAATGG CTTTTCATAT TCTGGCATTG 
GGTTTACTAG AAGAGAAGCA ACAGCTTCAA AAAGCTCCTG AAGAAGAAGT AACATTTGAC 
TTTTATCATA AGGCTTCAAG ATTGGGAAGT TCAGCCATGA ATATACAAAT GCTTTTGGAA 
AAACTCAAAG GAATTCCCCA GTTAGAAGGC CAGAAGGACA TGATAACGTG GATACTTCAG 
ATGTTTGACA CAGTGAAGCG ATTAAGAGAA AAATCTTGTT TAATTGTAGC AACCACATCA 
GGATCGGAAT CTATTAAGAA TGATGAGATT ACTCATGATA AAGAAAAAGC AGAACGAAAA 
AGAAAAGCTG AAGCTGCTAG GCTACATCGC CAGAAGATCA TGGCTCAGAT GTCTGCCTTA 
CAGAAAAACT TCATTGAAAC TCATAAACTC ATGTATGACA ATACATCAGA AATGCCTGGG 
AAAGAAGATT CCATTATGGA GGAAGAGAGC ACCCCAGCAG TCAGTGACTA CTCTAGAATT 
GCTTTGGGTC CTAAACGGGG TCCATCTGTT ACTGAAAAGG AGGTGCTGAC GTGCATCCTT 
TGCCAAGAAG AACAGGAGGT GAAAATAGAA AATAATGCCA TGGTATTATC GGCCTGTGTC 
CAGAAATCTA CTGCCTTAAC CCAGCACAGG GGAAAACCCA TAGAACTCTC AGGAGAAGCC 
CTAGACCCAC TTTTCATGGA TCCAGACTTG GCATATGGAA CTTATACAGG AAGCTGTGGT 
CATGTAATGC ACGCAGTGTG CTGGCAGAAG TATTTTGAAG CTGTACAGCT GAGCTCTCAG 
CAGCGCATTC ATGTTGACCT TTTTGACTTG GAAAGTGGAG AATATCTTTG CCCTCTTTGC 
AAATCTCTGT GCAATACTGT GATCCCCATT ATTCCTTTGC AACCTCAAAA GATAAACAGT 
GAGAATGCAG ATGCTCTTGC TCAACTTTTG ACCCTGGCAC GGTGGATACA GACTGTTCTG 
GCCAGAATAT CAGGTTATAA TATAAGACAT GCTAAAGGAG AAAACCCAAT TCCTATTTTC 
TTTAATCAAG GAATGGGAGA TTCTACTTTG GAGTTCCATT CCATCCTGAG TTTTGGCGTT 
GAGTCTTCGA TTAAATATTC AAATAGCATC AAGGAAATGG TTATTCTCTT TGCCACAACA 
ATTTATAGAA TTGGATTGAA AGTGCCACCT GATGAAAGGG ATCCTCGAGT CCCCATGCTG 
ACCTGGAGCA CCTGCGCTTT CACTATCCAG GCAATTGAAA ATCTATTGGG AGATGAAGGA 
AAACCTCTGT TTGGAGCACT TCAAAATAGG CAGCATAATG GTCTGAAAGC ATTAATGCAG 
TTTGCAGTTG CACAGAGGAT TACCTGTCCT CAGGTCCTGA TACAGAAACA TCTGGTTCGT 
CTTCTATCAG TTGTTCTTCC TAACATAAAA TCAGAAGATA CACCATGCCT TCTGTCTATA 
GATCTGTTTC ATGTTTTGGT GGGTGCTGTG TTAGCATTCC CATCCTTGTA TTGGGATGAC 
CCTGTTGATC TGCAGCCTTC TTCAGTTAGT TCTTCCTATA ACCACCTTTA TCTCTTCCAT 
TTGATCACCA TGGCACACAT GCTTCAGATA CTACTTACAG TAGACACAGG CCTACCCCTT 
GCTCAGGTTC AAGAAGACAG TGAAGAGGCT CATTCCGCAT CTTCTTTCTT TGCAGAAATT 
TCTCAATATA CAAGTGGCTC CATTGGGTGT GATATTCCTG GCTGGTATTT GTGGGTCTCA 
CTGAAGAATG GCATCACCCC TTATCTTCGC TGTGCTGCAT TGTTTTTCCA CTATTTACTT 
GGGGTAACTC CGCCTGAGGA ACTGCATACC AATTCTGCAG AAGGAGAGTA CAGTGCACTC 
TGTAGCTATC TATCTTTACC TACAAATTTG TTCCTGCTCT TCCAGGAATA TTGGGATACT 
GTAAGGCCCT TGCTCCAGAG GTGGTGTGCA GATCCTGCCT TACTAAACTG TTTGAAGCAA 
AAAAACACCG TGGTCAGGTA CCCTAGAAAA AGAAATAGTT TGATAGAGCT TCCTGATGAC 
TATAGCTGCC TCCTGAATCA AGCTTCTCAT TTCAGGTGCC CACGGTCTGC AGATGATGAG 
CGAAAGCATC CTGTCCTCTG CCTTTTCTGT GGGGCTATAC TATGTTCTCA GAACATTTGC 
TGCCAGGAAA TTGTGAACGG GGAAGAGGTT GGAGCTTGCA TTTTTCACGC ACTTCACTGT 
GGAGCCGGAG TCTGCATTTT CCTAAAAATC AGAGAATGCC GAGTGGTCCT GGTTGAAGGT 
AAAGCCAGAG GCTGTGCCTA TCCAGCTCCT TACTTGGATG AATATGGAGA AACAGACCCT 
GGCCTGAAGA GGGGCAACCC CCTTCATTTA TCTCGTGAGC GGTATCGGAA GCTCCATTTG 
GTCTGGCAAC AACACTGCAT TATAGAAGAG ATTGCTAGGA GCCAAGAGAC TAATCAGATG 
TTATTTGGAT TCAACTGGCA GTTACTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_777576.1
CDS	17..5266
Translation	MADEEAGGTERMEISAELPQTPQRLASWWDQQVDFYTAFLHHLAQLVPEIYFAEMDPDLEKQEESVQMSIFTPLEWYLFGEDPDICLEKLKHSGAFQLCGRVFKSGETTYSCRDCAIDPTCVLCMDCFQDSVHKNHRYKMHTSTGGGFCDCGDTEAWKTGPFCVNHEPGRAGTIKENSRCPLNEEVIVQARKIFPSVIKYVVEMTIWEEEKELPPELQIREKNERYYCVLFNDEHHSYDHVIYSLQRALDCELAEAQLHTTAIDKEGRRAVKAGAYAACQEAKEDIKSHSENVSQHPLHVEVLHSEIMAHQKFALRLGSWMNKIMSYSSDFRQIFCQACLREEPDSENPCLISRLMLWDAKLYKGARKILHELIFSSFFMEMEYKKLFAMEFVKYYKQLQKEYISDDHDRSISITALSVQMFTVPTLARHLIEEQNVISVITETLLEVLPEYLDRNNKFNFQGYSQDKLGRVYAVICDLKYILISKPTIWTERLRMQFLEGFRSFLKILTCMQGMEEIRRQVGQHIEVDPDWEAAIAIQMQLKNILLMFQEWCACDEELLLVAYKECHKAVMRCSTSFISSSKTVVQSCGHSLETKSYRVSEDLVSIHLPLSRTLAGLHVRLSRLGAVSRLHEFVSFEDFQVEVLVEYPLRCLVLVAQVVAEMWRRNGLSLISQVFYYQDVKCREEMYDKDIIMLQIGASLMDPNKFLLLVLQRYELAEAFNKTISTKDQDLIKQYNTLIEEMLQVLIYIVGERYVPGVGNVTKEEVTMREIIHLLCIEPMPHSAIAKNLPENENNETGLENVINKVATFKKPGVSGHGVYELKDESLKDFNMYFYHYSKTQHSKAEHMQKKRRKQENKDEALPPPPPPEFCPAFSKVINLLNCDIMMYILRTVFERAIDTDSNLWTEGMLQMAFHILALGLLEEKQQLQKAPEEEVTFDFYHKASRLGSSAMNIQMLLEKLKGIPQLEGQKDMITWILQMFDTVKRLREKSCLIVATTSGSESIKNDEITHDKEKAERKRKAEAARLHRQKIMAQMSALQKNFIETHKLMYDNTSEMPGKEDSIMEEESTPAVSDYSRIALGPKRGPSVTEKEVLTCILCQEEQEVKIENNAMVLSACVQKSTALTQHRGKPIELSGEALDPLFMDPDLAYGTYTGSCGHVMHAVCWQKYFEAVQLSSQQRIHVDLFDLESGEYLCPLCKSLCNTVIPIIPLQPQKINSENADALAQLLTLARWIQTVLARISGYNIRHAKGENPIPIFFNQGMGDSTLEFHSILSFGVESSIKYSNSIKEMVILFATTIYRIGLKVPPDERDPRVPMLTWSTCAFTIQAIENLLGDEGKPLFGALQNRQHNGLKALMQFAVAQRITCPQVLIQKHLVRLLSVVLPNIKSEDTPCLLSIDLFHVLVGAVLAFPSLYWDDPVDLQPSSVSSSYNHLYLFHLITMAHMLQILLTVDTGLPLAQVQEDSEEAHSASSFFAEISQYTSGSIGCDIPGWYLWVSLKNGITPYLRCAALFFHYLLGVTPPEELHTNSAEGEYSALCSYLSLPTNLFLLFQEYWDTVRPLLQRWCADPALLNCLKQKNTVVRYPRKRNSLIELPDDYSCLLNQASHFRCPRSADDERKHPVLCLFCGAILCSQNICCQEIVNGEEVGACIFHALHCGAGVCIFLKIRECRVVLVEGKARGCAYPAPYLDEYGETDPGLKRGNPLHLSRERYRKLHLVWQQHCIIEEIARSQETNQMLFGFNWQLL

Target ORF information:

RefSeq Version	NM_174916.3
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin protein ligase E3 component n-recognin 1 (UBR1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_174916.3

ORF Insert Sequence:

ATGGCGGACG AGGAGGCTGG AGGTACTGAG AGGATGGAAA TCAGCGCGGA GTTACCCCAG 
ACCCCTCAGC GTCTGGCATC TTGGTGGGAT CAGCAAGTTG ATTTTTATAC TGCTTTCTTG 
CATCATTTGG CACAATTGGT GCCAGAAATT TACTTTGCTG AAATGGACCC AGACTTGGAA 
AAGCAGGAGG AAAGTGTACA AATGTCAATA TTCACTCCAC TGGAATGGTA CTTATTTGGA 
GAAGATCCAG ATATTTGCTT AGAGAAATTG AAGCACAGTG GAGCATTTCA GCTTTGTGGG 
AGGGTTTTCA AAAGTGGAGA GACAACCTAT TCTTGCAGGG ATTGTGCAAT TGATCCAACA 
TGTGTACTCT GTATGGACTG CTTCCAGGAC AGTGTTCATA AAAATCATCG TTACAAGATG 
CATACTTCTA CTGGAGGAGG GTTCTGTGAC TGTGGAGACA CAGAGGCATG GAAAACTGGC 
CCTTTTTGTG TAAATCATGA ACCTGGAAGA GCAGGTACTA TAAAAGAGAA TTCACGCTGT 
CCGTTGAATG AAGAGGTAAT TGTCCAAGCC AGGAAAATAT TTCCTTCAGT GATAAAATAT 
GTCGTAGAAA TGACTATATG GGAAGAGGAA AAAGAACTGC CTCCTGAACT CCAGATAAGG 
GAGAAAAATG AAAGATACTA TTGTGTCCTT TTCAATGATG AACACCATTC ATATGACCAC 
GTCATATACA GCCTACAAAG AGCTCTTGAC TGTGAGCTCG CAGAGGCCCA GTTGCATACC 
ACTGCCATTG ACAAAGAGGG TCGTCGGGCT GTTAAAGCGG GAGCTTATGC TGCTTGCCAG 
GAAGCAAAGG AAGATATAAA GAGTCATTCA GAAAATGTCT CTCAACATCC ACTTCATGTA 
GAAGTATTAC ACTCAGAGAT TATGGCTCAT CAGAAATTTG CTTTGCGTCT TGGTTCCTGG 
ATGAACAAAA TTATGAGCTA TTCAAGTGAC TTTAGGCAGA TCTTTTGCCA AGCATGCCTT 
AGAGAAGAAC CTGACTCGGA GAATCCCTGT CTCATAAGCA GGTTAATGCT TTGGGATGCA 
AAGCTTTATA AAGGTGCCCG TAAGATCCTT CATGAATTGA TCTTCAGCAG TTTTTTTATG 
GAGATGGAAT ACAAAAAACT CTTTGCTATG GAATTTGTGA AGTATTATAA ACAACTGCAG 
AAAGAATATA TCAGTGATGA TCATGACAGA AGTATCTCTA TAACTGCACT TTCAGTTCAG 
ATGTTTACTG TTCCTACTCT GGCTCGACAT CTTATTGAAG AGCAGAATGT TATCTCTGTC 
ATTACTGAAA CTCTGCTAGA AGTTTTACCT GAGTACTTGG ACAGGAACAA TAAATTCAAC 
TTCCAGGGTT ATAGCCAGGA CAAATTGGGA AGAGTATATG CAGTAATATG TGACCTAAAG 
TATATCCTGA TCAGCAAACC CACAATATGG ACAGAAAGAT TAAGAATGCA GTTCCTTGAA 
GGTTTTCGAT CTTTTTTGAA GATTCTTACC TGTATGCAGG GAATGGAAGA AATCCGAAGA 
CAGGTTGGGC AACACATTGA AGTGGATCCT GATTGGGAGG CTGCCATTGC TATACAGATG 
CAATTGAAGA ATATTTTACT CATGTTCCAA GAGTGGTGTG CTTGTGATGA AGAACTCTTA 
CTTGTGGCTT ATAAAGAATG TCACAAAGCT GTGATGAGGT GCAGTACCAG TTTCATATCT 
AGTAGCAAGA CAGTAGTACA ATCGTGTGGA CATAGTTTGG AAACAAAGTC CTACAGAGTA 
TCTGAGGATC TTGTAAGCAT ACATCTGCCA CTCTCTAGGA CCCTTGCTGG TCTTCATGTA 
CGTTTAAGCA GGCTGGGTGC TGTTTCAAGA CTGCATGAAT TTGTGTCTTT TGAGGACTTT 
CAAGTAGAGG TACTAGTGGA ATATCCTTTA CGTTGTCTGG TGTTGGTTGC CCAGGTTGTT 
GCTGAGATGT GGCGAAGAAA TGGACTGTCT CTTATTAGCC AGGTGTTTTA TTACCAAGAT 
GTTAAGTGCA GAGAAGAAAT GTATGATAAA GATATCATCA TGCTTCAGAT TGGTGCATCT 
TTAATGGATC CCAATAAGTT CTTGTTACTG GTACTTCAGA GGTATGAACT TGCCGAGGCT 
TTTAACAAGA CCATATCTAC AAAAGACCAG GATTTGATTA AACAATATAA TACACTAATA 
GAAGAAATGC TTCAGGTCCT CATCTATATT GTGGGTGAGC GTTATGTACC TGGAGTGGGA 
AATGTGACCA AAGAAGAGGT CACAATGAGA GAAATCATTC ACTTGCTTTG CATTGAACCC 
ATGCCACACA GTGCCATTGC CAAAAATTTA CCTGAGAATG AAAATAATGA AACTGGCTTA 
GAGAATGTCA TAAACAAAGT GGCCACATTT AAGAAACCAG GTGTATCAGG CCATGGAGTT 
TATGAACTAA AAGATGAATC ACTGAAAGAC TTCAATATGT ACTTTTATCA TTACTCCAAA 
ACCCAGCATA GCAAGGCTGA ACATATGCAG AAGAAAAGGA GAAAACAAGA AAACAAAGAT 
GAAGCATTGC CGCCACCACC ACCTCCTGAA TTCTGCCCTG CTTTCAGCAA AGTGATTAAC 
CTTCTCAACT GTGATATCAT GATGTACATT CTCAGGACCG TATTTGAGCG GGCAATAGAC 
ACAGATTCTA ACTTGTGGAC CGAAGGGATG CTCCAAATGG CTTTTCATAT TCTGGCATTG 
GGTTTACTAG AAGAGAAGCA ACAGCTTCAA AAAGCTCCTG AAGAAGAAGT AACATTTGAC 
TTTTATCATA AGGCTTCAAG ATTGGGAAGT TCAGCCATGA ATATACAAAT GCTTTTGGAA 
AAACTCAAAG GAATTCCCCA GTTAGAAGGC CAGAAGGACA TGATAACGTG GATACTTCAG 
ATGTTTGACA CAGTGAAGCG ATTAAGAGAA AAATCTTGTT TAATTGTAGC AACCACATCA 
GGATCGGAAT CTATTAAGAA TGATGAGATT ACTCATGATA AAGAAAAAGC AGAACGAAAA 
AGAAAAGCTG AAGCTGCTAG GCTACATCGC CAGAAGATCA TGGCTCAGAT GTCTGCCTTA 
CAGAAAAACT TCATTGAAAC TCATAAACTC ATGTATGACA ATACATCAGA AATGCCTGGG 
AAAGAAGATT CCATTATGGA GGAAGAGAGC ACCCCAGCAG TCAGTGACTA CTCTAGAATT 
GCTTTGGGTC CTAAACGGGG TCCATCTGTT ACTGAAAAGG AGGTGCTGAC GTGCATCCTT 
TGCCAAGAAG AACAGGAGGT GAAAATAGAA AATAATGCCA TGGTATTATC GGCCTGTGTC 
CAGAAATCTA CTGCCTTAAC CCAGCACAGG GGAAAACCCA TAGAACTCTC AGGAGAAGCC 
CTAGACCCAC TTTTCATGGA TCCAGACTTG GCATATGGAA CTTATACAGG AAGCTGTGGT 
CATGTAATGC ACGCAGTGTG CTGGCAGAAG TATTTTGAAG CTGTACAGCT GAGCTCTCAG 
CAGCGCATTC ATGTTGACCT TTTTGACTTG GAAAGTGGAG AATATCTTTG CCCTCTTTGC 
AAATCTCTGT GCAATACTGT GATCCCCATT ATTCCTTTGC AACCTCAAAA GATAAACAGT 
GAGAATGCAG ATGCTCTTGC TCAACTTTTG ACCCTGGCAC GGTGGATACA GACTGTTCTG 
GCCAGAATAT CAGGTTATAA TATAAGACAT GCTAAAGGAG AAAACCCAAT TCCTATTTTC 
TTTAATCAAG GAATGGGAGA TTCTACTTTG GAGTTCCATT CCATCCTGAG TTTTGGCGTT 
GAGTCTTCGA TTAAATATTC AAATAGCATC AAGGAAATGG TTATTCTCTT TGCCACAACA 
ATTTATAGAA TTGGATTGAA AGTGCCACCT GATGAAAGGG ATCCTCGAGT CCCCATGCTG 
ACCTGGAGCA CCTGCGCTTT CACTATCCAG GCAATTGAAA ATCTATTGGG AGATGAAGGA 
AAACCTCTGT TTGGAGCACT TCAAAATAGG CAGCATAATG GTCTGAAAGC ATTAATGCAG 
TTTGCAGTTG CACAGAGGAT TACCTGTCCT CAGGTCCTGA TACAGAAACA TCTGGTTCGT 
CTTCTATCAG TTGTTCTTCC TAACATAAAA TCAGAAGATA CACCATGCCT TCTGTCTATA 
GATCTGTTTC ATGTTTTGGT GGGTGCTGTG TTAGCATTCC CATCCTTGTA TTGGGATGAC 
CCTGTTGATC TGCAGCCTTC TTCAGTTAGT TCTTCCTATA ACCACCTTTA TCTCTTCCAT 
TTGATCACCA TGGCACACAT GCTTCAGATA CTACTTACAG TAGACACAGG CCTACCCCTT 
GCTCAGGTTC AAGAAGACAG TGAAGAGGCT CATTCCGCAT CTTCTTTCTT TGCAGAAATT 
TCTCAATATA CAAGTGGCTC CATTGGGTGT GATATTCCTG GCTGGTATTT GTGGGTCTCA 
CTGAAGAATG GCATCACCCC TTATCTTCGC TGTGCTGCAT TGTTTTTCCA CTATTTACTT 
GGGGTAACTC CGCCTGAGGA ACTGCATACC AATTCTGCAG AAGGAGAGTA CAGTGCACTC 
TGTAGCTATC TATCTTTACC TACAAATTTG TTCCTGCTCT TCCAGGAATA TTGGGATACT 
GTAAGGCCCT TGCTCCAGAG GTGGTGTGCA GATCCTGCCT TACTAAACTG TTTGAAGCAA 
AAAAACACCG TGGTCAGGTA CCCTAGAAAA AGAAATAGTT TGATAGAGCT TCCTGATGAC 
TATAGCTGCC TCCTGAATCA AGCTTCTCAT TTCAGGTGCC CACGGTCTGC AGATGATGAG 
CGAAAGCATC CTGTCCTCTG CCTTTTCTGT GGGGCTATAC TATGTTCTCA GAACATTTGC 
TGCCAGGAAA TTGTGAACGG GGAAGAGGTT GGAGCTTGCA TTTTTCACGC ACTTCACTGT 
GGAGCCGGAG TCTGCATTTT CCTAAAAATC AGAGAATGCC GAGTGGTCCT GGTTGAAGGT 
AAAGCCAGAG GCTGTGCCTA TCCAGCTCCT TACTTGGATG AATATGGAGA AACAGACCCT 
GGCCTGAAGA GGGGCAACCC CCTTCATTTA TCTCGTGAGC GGTATCGGAA GCTCCATTTG 
GTCTGGCAAC AACACTGCAT TATAGAAGAG ATTGCTAGGA GCCAAGAGAC TAATCAGATG 
TTATTTGGAT TCAACTGGCA GTTACTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Expanding the mutational spectrum in Johanson-Blizzard syndrome: identification of whole exon deletions and duplications in the UBR1 gene by multiplex ligation-dependent probe amplification analysis.
Molecular genetics & genomic medicine5(6)774-780(2017 Nov)
Sukalo M,Sch?flein E,Schanze I,Everman DB,Rezaei N,Argente J,Lorda-Sanchez I,Deshpande C,Takahashi T,Kleger A,Zenker M

Variants in the UBR1 gene are not associated with chronic pancreatitis in Japan.
Pancreatology : official journal of the International Association of Pancreatology (IAP) ... [et al.]16(5)814-8(2016 Sep-Oct)
Masamune A,Nakano E,Niihori T,Hamada S,Nagasaki M,Aoki Y,Shimosegawa T

Implication of a Chromosome 15q15.2 Locus in Regulating UBR1 and Predisposing Smokers to MGMT Methylation in Lung.
Cancer research75(15)3108-17(2015 Aug)
Leng S,Wu G,Collins LB,Thomas CL,Tellez CS,Jauregui AR,Picchi MA,Zhang X,Juri DE,Desai D,Amin SG,Crowell RE,Stidley CA,Liu Y,Swenberg JA,Lin Y,Wathelet MG,Gilliland FD,Belinsky SA

Mutations in the human UBR1 gene and the associated phenotypic spectrum.
Human mutation35(5)521-31(2014 May)
Sukalo M,Fiedler A,Guzm?n C,Spranger S,Addor MC,McHeik JN,Oltra Benavent M,Cobben JM,Gillis LA,Shealy AG,Deshpande C,Bozorgmehr B,Everman DB,Stattin EL,Liebelt J,Keller KM,Bertola DR,van Karnebeek CD,Bergmann C,Liu Z,D?ker G,Rezaei N,Alkuraya FS,O?ur G,Alrajoudi A,Venegas-Vega CA,Verbeek NE,Richmond EJ,Kirbiyik O,Ranganath P,Singh A,Godbole K,Ali FA,Alves C,Mayerle J,Lerch MM,Witt H,Zenker M

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UBR1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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