EIF2B1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	EIF2B1
Entrez Gene ID	1967
Full Name	eukaryotic translation initiation factor 2B subunit alpha
Synonyms	EIF2B,EIF2BA
General protein information	Preferred Names eukaryotic translation initiation factor 2B subunit alpha Names translation initiation factor eIF-2B subunit alpha eIF-2B GDP-GTP exchange factor subunit alpha eukaryotic translation initiation factor 2B, subunit 1 alpha, 26kDa
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 12 Map Location: 12q24.31
Summary	This gene encodes one of five subunits of eukaryotic translation initiation factor 2B (EIF2B), a GTP exchange factor for eukaryotic initiation factor 2 and an essential regulator for protein synthesis. Mutations in this gene and the genes encoding other EIF2B subunits have been associated with leukoencephalopathy with vanishing white matter. [provided by RefSeq, Oct 2009].
Disorder MIM:	606686
Disorder Html:	Leukoencephalopathy with vanishing white matter, 603896 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001414.3	NP_001405.1	translation initiation factor eIF-2B subunit alpha
NM_001414.4	NP_001405.1	translation initiation factor eIF-2B subunit alpha

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	LOC100151592	XP_005168723.1
Drosophila melanogaster (fruit fly)	eIF2B-alpha	NP_651752.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP012611	XP_307038.2
Kluyveromyces lactis	KLLA0F13970g	XP_455709.2
Mus musculus (house mouse)	Eif2b1	NP_663346.1
Gallus gallus (chicken)	EIF2B1	XP_003642240.1
Caenorhabditis elegans (roundworm)	ZK1098.4	NP_499106.1
Saccharomyces cerevisiae (baker's yeast)	GCN3	NP_012951.1
Eremothecium gossypii	AGOS_AFR084C	NP_985631.2
Bos taurus (cattle)	EIF2B1	NP_001069211.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003650	XP_313420.3
Arabidopsis thaliana (thale cress)	AT1G53900	NP_564646.3
	EIF2B1	XP_001101480.2
Oryza sativa (rice)	Os12g0497400	NP_001066807.1
Xenopus tropicalis (tropical clawed frog)	eif2b1	NP_001017286.1
Homo sapiens (human)	EIF2B1	NP_001405.1
Pan troglodytes (chimpanzee)	EIF2B1	XP_522583.3
Canis lupus familiaris (dog)	EIF2B1	XP_005636171.1
Rattus norvegicus (Norway rat)	Eif2b1	NP_742026.1
Danio rerio (zebrafish)	eif2b1	NP_001002060.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP012799	XP_307097.4
Schizosaccharomyces pombe (fission yeast)	SPCC11E10.07c	NP_588203.1
Arabidopsis thaliana (thale cress)	AT1G72340	NP_565041.1
Arabidopsis thaliana (thale cress)	AT1G53880	NP_564643.2

Gene Ontology
Bibliography

Related articles in PubMed

Novel mechanisms of eIF2B action and regulation by eIF2? phosphorylation.
Bogorad AM, Lin KY, Marintchev A
Nucleic acids research45(20)11962-11979(2017 Nov)

Different Eukaryotic Initiation Factor 2B? Mutations Lead to Various Degrees of Intolerance to the Stress of Endoplasmic Reticulum in Oligodendrocytes.
Chen N, Jiang YW, Hao HJ, Ban TT, Gao K, Zhang ZB, Wang JM, Wu Y
Chinese medical journal128(13)1772-7(2015 Jul)

The alpha subunit of eukaryotic initiation factor 2B (eIF2B) is required for eIF2-mediated translational suppression of vesicular stomatitis virus.
Elsby R, Heiber JF, Reid P, Kimball SR, Pavitt GD, Barber GN
Journal of virology85(19)9716-25(2011 Oct)

Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study.
Bailey SD, Xie C, Do R, Montpetit A, Diaz R, Mohan V, Keavney B, Yusuf S, Gerstein HC, Engert JC, Anand S,
Diabetes care33(10)2250-3(2010 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data show that eIF2Balpha and eIF2Bbeta bind to adjacent surfaces on eIF2alpha-N-terminal domains (NTDs).
Title: Novel mechanisms of eIF2B action and regulation by eIF2? phosphorylation.

Oligodendrocytes with truncated or deletion mutants eIF2B showed less tolerable to endoplasmic reticulum stress
Title: Different Eukaryotic Initiation Factor 2B? Mutations Lead to Various Degrees of Intolerance to the Stress of Endoplasmic Reticulum in Oligodendrocytes.

These data emphasize the importance of eIF2Balpha in mediating the eIF2 kinase translation-inhibitory activity and may provide insight into the complex nature of vesiculovirus oncolysis.
Title: The alpha subunit of eukaryotic initiation factor 2B (eIF2B) is required for eIF2-mediated translational suppression of vesicular stomatitis virus.

Observational study of gene-disease association, gene-environment interaction, and pharmacogenomic / toxicogenomic. (HuGE Navigator)
Title: Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study.

Observational study of gene-disease association. (HuGE Navigator)
Title: Gene-centric association signals for lipids and apolipoproteins identified via the HumanCVD BeadChip.

The following EIF2B1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EIF2B1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu26433	NM_001414.4 Latest version!	Homo sapiens eukaryotic translation initiation factor 2B subunit alpha (EIF2B1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$99.50-$139.30 $199.00
View Old Accession Versions >>
OHu26433	NM_001414.3	Homo sapiens eukaryotic translation initiation factor 2B subunit alpha (EIF2B1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$99.50-$139.30 $199.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

EIF2B1 ( NM_001414.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001405.1 Homo sapiens eukaryotic translation initiation factor 2B subunit alpha (EIF2B1), mRNA.

Price & Availability↑

CloneID	OHu26433
Clone ID Related Accession (Same CDS sequence)	NM_001414.3 , NM_001414.4
Accession Version	NM_001414.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 918bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-21
Organism	Homo sapiens(human)
Product	translation initiation factor eIF-2B subunit alpha
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff in collaboration with Francesco Amaldi. The reference sequence was derived from DA879094.1, DA221985.1, BC103763.1 and AI540796.1. This sequence is a reference standard in the RefSeqGene project. On Oct 31, 2009 this sequence version replaced NM_001414.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.37169.1, SRR1660809.158728.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: full length.

ATGGACGACA AGGAGTTAAT TGAATACTTT AAGTCTCAGA TGAAAGAAGA TCCTGACATG 
GCCTCAGCAG TGGCTGCCAT CCGGACGTTG CTGGAGTTCT TGAAGAGAGA TAAAGGGGAG 
ACAATCCAGG GTCTGAGGGC GAATCTCACC AGTGCCATAG AAACCCTGTG TGGTGTGGAC 
TCCTCTGTGG CAGTGTCCTC TGGCGGGGAG CTCTTCCTCC GCTTCATCAG TCTTGCCTCC 
CTGGAATACT CCGATTACTC CAAATGTAAA AAGATCATGA TTGAGCGGGG AGAACTTTTT 
CTCAGGAGAA TATCACTGTC AAGAAACAAA ATTGCAGATC TGTGCCATAC TTTCATCAAA 
GATGGAGCGA CAATATTGAC TCACGCCTAC TCCAGAGTGG TCCTGAGAGT CCTGGAAGCA 
GCCGTGGCGG CCAAGAAGCG ATTTAGTGTA TACGTCACAG AGTCACAGCC TGATTTGTCA 
GGTAAGAAAA TGGCCAAAGC CCTCTGCCAC CTCAACGTCC CTGTCACTGT GGTGCTAGAT 
GCTGCTGTCG GCTACATCAT GGAGAAAGCA GATCTTGTCA TAGTTGGTGC TGAAGGAGTT 
GTTGAAAACG GAGGAATTAT TAACAAGATT GGAACCAACC AGATGGCTGT GTGTGCCAAA 
GCACAGAACA AACCTTTCTA TGTGGTTGCA GAAAGTTTCA AGTTTGTCCG GCTCTTTCCA 
CTAAACCAGC AAGACGTCCC AGATAAGTTT AAGTATAAGG CAGACACTCT CAAGGTCGCG 
CAGACTGGAC AAGACCTCAA AGAGGAGCAT CCGTGGGTCG ACTACACTGC CCCTTCCTTA 
ATCACTCTGC TGTTTACAGA CCTGGGCGTG CTGACACCCT CAGCAGTCAG CGATGAGCTC 
ATCAAGCTCT ATCTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001405.1
CDS	220..1137
Translation	MDDKELIEYFKSQMKEDPDMASAVAAIRTLLEFLKRDKGETIQGLRANLTSAIETLCGVDSSVAVSSGGELFLRFISLASLEYSDYSKCKKIMIERGELFLRRISLSRNKIADLCHTFIKDGATILTHAYSRVVLRVLEAAVAAKKRFSVYVTESQPDLSGKKMAKALCHLNVPVTVVLDAAVGYIMEKADLVIVGAEGVVENGGIINKIGTNQMAVCAKAQNKPFYVVAESFKFVRLFPLNQQDVPDKFKYKADTLKVAQTGQDLKEEHPWVDYTAPSLITLLFTDLGVLTPSAVSDELIKLYL

Target ORF information:

RefSeq Version	NM_001414.3
Organism	Homo sapiens(human)
Definition	Homo sapiens eukaryotic translation initiation factor 2B subunit alpha (EIF2B1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001414.3

ORF Insert Sequence:

ATGGACGACA AGGAGTTAAT TGAATACTTT AAGTCTCAGA TGAAAGAAGA TCCTGACATG 
GCCTCAGCAG TGGCTGCCAT CCGGACGTTG CTGGAGTTCT TGAAGAGAGA TAAAGGGGAG 
ACAATCCAGG GTCTGAGGGC GAATCTCACC AGTGCCATAG AAACCCTGTG TGGTGTGGAC 
TCCTCTGTGG CAGTGTCCTC TGGCGGGGAG CTCTTCCTCC GCTTCATCAG TCTTGCCTCC 
CTGGAATACT CCGATTACTC CAAATGTAAA AAGATCATGA TTGAGCGGGG AGAACTTTTT 
CTCAGGAGAA TATCACTGTC AAGAAACAAA ATTGCAGATC TGTGCCATAC TTTCATCAAA 
GATGGAGCGA CAATATTGAC TCACGCCTAC TCCAGAGTGG TCCTGAGAGT CCTGGAAGCA 
GCCGTGGCGG CCAAGAAGCG ATTTAGTGTA TACGTCACAG AGTCACAGCC TGATTTGTCA 
GGTAAGAAAA TGGCCAAAGC CCTCTGCCAC CTCAACGTCC CTGTCACTGT GGTGCTAGAT 
GCTGCTGTCG GCTACATCAT GGAGAAAGCA GATCTTGTCA TAGTTGGTGC TGAAGGAGTT 
GTTGAAAACG GAGGAATTAT TAACAAGATT GGAACCAACC AGATGGCTGT GTGTGCCAAA 
GCACAGAACA AACCTTTCTA TGTGGTTGCA GAAAGTTTCA AGTTTGTCCG GCTCTTTCCA 
CTAAACCAGC AAGACGTCCC AGATAAGTTT AAGTATAAGG CAGACACTCT CAAGGTCGCG 
CAGACTGGAC AAGACCTCAA AGAGGAGCAT CCGTGGGTCG ACTACACTGC CCCTTCCTTA 
ATCACTCTGC TGTTTACAGA CCTGGGCGTG CTGACACCCT CAGCAGTCAG CGATGAGCTC 
ATCAAGCTCT ATCTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EIF2B1 ( NM_001414.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_001405.1 Homo sapiens eukaryotic translation initiation factor 2B subunit alpha (EIF2B1), mRNA.

Price & Availability↑

CloneID	OHu26433
Clone ID Related Accession (Same CDS sequence)	NM_001414.3 , NM_001414.4
Accession Version	NM_001414.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 918bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-24
Organism	Homo sapiens(human)
Product	translation initiation factor eIF-2B subunit alpha
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff in collaboration with Francesco Amaldi. The reference sequence was derived from AC117503.8. On May 17, 2019 this sequence version replaced NM_001414.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.5290.1, SRR1660807.189405.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000424014.7/ ENSP00000416250.2 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGACGACA AGGAGTTAAT TGAATACTTT AAGTCTCAGA TGAAAGAAGA TCCTGACATG 
GCCTCAGCAG TGGCTGCCAT CCGGACGTTG CTGGAGTTCT TGAAGAGAGA TAAAGGGGAG 
ACAATCCAGG GTCTGAGGGC GAATCTCACC AGTGCCATAG AAACCCTGTG TGGTGTGGAC 
TCCTCTGTGG CAGTGTCCTC TGGCGGGGAG CTCTTCCTCC GCTTCATCAG TCTTGCCTCC 
CTGGAATACT CCGATTACTC CAAATGTAAA AAGATCATGA TTGAGCGGGG AGAACTTTTT 
CTCAGGAGAA TATCACTGTC AAGAAACAAA ATTGCAGATC TGTGCCATAC TTTCATCAAA 
GATGGAGCGA CAATATTGAC TCACGCCTAC TCCAGAGTGG TCCTGAGAGT CCTGGAAGCA 
GCCGTGGCGG CCAAGAAGCG ATTTAGTGTA TACGTCACAG AGTCACAGCC TGATTTGTCA 
GGTAAGAAAA TGGCCAAAGC CCTCTGCCAC CTCAACGTCC CTGTCACTGT GGTGCTAGAT 
GCTGCTGTCG GCTACATCAT GGAGAAAGCA GATCTTGTCA TAGTTGGTGC TGAAGGAGTT 
GTTGAAAACG GAGGAATTAT TAACAAGATT GGAACCAACC AGATGGCTGT GTGTGCCAAA 
GCACAGAACA AACCTTTCTA TGTGGTTGCA GAAAGTTTCA AGTTTGTCCG GCTCTTTCCA 
CTAAACCAGC AAGACGTCCC AGATAAGTTT AAGTATAAGG CAGACACTCT CAAGGTCGCG 
CAGACTGGAC AAGACCTCAA AGAGGAGCAT CCGTGGGTCG ACTACACTGC CCCTTCCTTA 
ATCACTCTGC TGTTTACAGA CCTGGGCGTG CTGACACCCT CAGCAGTCAG CGATGAGCTC 
ATCAAGCTCT ATCTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001405.1
CDS	130..1047
Translation	MDDKELIEYFKSQMKEDPDMASAVAAIRTLLEFLKRDKGETIQGLRANLTSAIETLCGVDSSVAVSSGGELFLRFISLASLEYSDYSKCKKIMIERGELFLRRISLSRNKIADLCHTFIKDGATILTHAYSRVVLRVLEAAVAAKKRFSVYVTESQPDLSGKKMAKALCHLNVPVTVVLDAAVGYIMEKADLVIVGAEGVVENGGIINKIGTNQMAVCAKAQNKPFYVVAESFKFVRLFPLNQQDVPDKFKYKADTLKVAQTGQDLKEEHPWVDYTAPSLITLLFTDLGVLTPSAVSDELIKLYL

Target ORF information:

RefSeq Version	NM_001414.4
Organism	Homo sapiens(human)
Definition	Homo sapiens eukaryotic translation initiation factor 2B subunit alpha (EIF2B1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001414.4

ORF Insert Sequence:

ATGGACGACA AGGAGTTAAT TGAATACTTT AAGTCTCAGA TGAAAGAAGA TCCTGACATG 
GCCTCAGCAG TGGCTGCCAT CCGGACGTTG CTGGAGTTCT TGAAGAGAGA TAAAGGGGAG 
ACAATCCAGG GTCTGAGGGC GAATCTCACC AGTGCCATAG AAACCCTGTG TGGTGTGGAC 
TCCTCTGTGG CAGTGTCCTC TGGCGGGGAG CTCTTCCTCC GCTTCATCAG TCTTGCCTCC 
CTGGAATACT CCGATTACTC CAAATGTAAA AAGATCATGA TTGAGCGGGG AGAACTTTTT 
CTCAGGAGAA TATCACTGTC AAGAAACAAA ATTGCAGATC TGTGCCATAC TTTCATCAAA 
GATGGAGCGA CAATATTGAC TCACGCCTAC TCCAGAGTGG TCCTGAGAGT CCTGGAAGCA 
GCCGTGGCGG CCAAGAAGCG ATTTAGTGTA TACGTCACAG AGTCACAGCC TGATTTGTCA 
GGTAAGAAAA TGGCCAAAGC CCTCTGCCAC CTCAACGTCC CTGTCACTGT GGTGCTAGAT 
GCTGCTGTCG GCTACATCAT GGAGAAAGCA GATCTTGTCA TAGTTGGTGC TGAAGGAGTT 
GTTGAAAACG GAGGAATTAT TAACAAGATT GGAACCAACC AGATGGCTGT GTGTGCCAAA 
GCACAGAACA AACCTTTCTA TGTGGTTGCA GAAAGTTTCA AGTTTGTCCG GCTCTTTCCA 
CTAAACCAGC AAGACGTCCC AGATAAGTTT AAGTATAAGG CAGACACTCT CAAGGTCGCG 
CAGACTGGAC AAGACCTCAA AGAGGAGCAT CCGTGGGTCG ACTACACTGC CCCTTCCTTA 
ATCACTCTGC TGTTTACAGA CCTGGGCGTG CTGACACCCT CAGCAGTCAG CGATGAGCTC 
ATCAAGCTCT ATCTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Novel mechanisms of eIF2B action and regulation by eIF2? phosphorylation.
Nucleic acids research45(20)11962-11979(2017 Nov)
Bogorad AM,Lin KY,Marintchev A

Different Eukaryotic Initiation Factor 2B? Mutations Lead to Various Degrees of Intolerance to the Stress of Endoplasmic Reticulum in Oligodendrocytes.
Chinese medical journal128(13)1772-7(2015 Jul)
Chen N,Jiang YW,Hao HJ,Ban TT,Gao K,Zhang ZB,Wang JM,Wu Y

The alpha subunit of eukaryotic initiation factor 2B (eIF2B) is required for eIF2-mediated translational suppression of vesicular stomatitis virus.
Journal of virology85(19)9716-25(2011 Oct)
Elsby R,Heiber JF,Reid P,Kimball SR,Pavitt GD,Barber GN

Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study.
Diabetes care33(10)2250-3(2010 Oct)
Bailey SD,Xie C,Do R,Montpetit A,Diaz R,Mohan V,Keavney B,Yusuf S,Gerstein HC,Engert JC,Anand S,

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EIF2B1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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