EGF gene cDNA ORF clone, Homo sapiens(human)

mRNA	Protein	Name
XM_017007851.1	XP_016863340.1	pro-epidermal growth factor isoform X9
NM_001963.6	NP_001954.2	pro-epidermal growth factor isoform 1 preproprotein
NM_001963.5	NP_001954.2	pro-epidermal growth factor isoform 1 preproprotein
XM_017007852.1	XP_016863341.1	pro-epidermal growth factor isoform X10
XM_017007845.1	XP_016863334.1	pro-epidermal growth factor isoform X1
XM_017007848.1	XP_016863337.1	pro-epidermal growth factor isoform X5
XM_017007849.1	XP_016863338.1	pro-epidermal growth factor isoform X7
XM_017007853.1	XP_016863342.1	pro-epidermal growth factor isoform X10
NM_001178130.2	NP_001171601.1	pro-epidermal growth factor isoform 2 precursor
XM_005262796.2	XP_005262853.1	pro-epidermal growth factor isoform X2
XM_017007846.1	XP_016863335.1	pro-epidermal growth factor isoform X3
XM_017007847.1	XP_016863336.1	pro-epidermal growth factor isoform X4
NM_001357021.2	NP_001343950.1	pro-epidermal growth factor isoform 4 precursor
NM_001178130.3	NP_001171601.1	pro-epidermal growth factor isoform 2 precursor
NM_001178131.3	NP_001171602.1	pro-epidermal growth factor isoform 3 precursor
NM_001357021.1	NP_001343950.1	pro-epidermal growth factor isoform 4 precursor
NM_001178131.2	NP_001171602.1	pro-epidermal growth factor isoform 3 precursor
XM_017007850.1	XP_016863339.1	pro-epidermal growth factor isoform X8
XM_017007854.1	XP_016863343.1	pro-epidermal growth factor isoform X11
XM_017007855.1	XP_016863344.1	pro-epidermal growth factor isoform X12
XM_011531707.1	XP_011530009.1	pro-epidermal growth factor isoform X6

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu23582	NM_001963.6 Latest version!	Homo sapiens epidermal growth factor (EGF), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$274.50-$384.30 $549.00
OHu94958	XM_017007853.1 Latest version!	Homo sapiens epidermal growth factor (EGF), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OHu94959	XM_017007854.1 Latest version!	Homo sapiens epidermal growth factor (EGF), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OHu94960	XM_017007855.1 Latest version!	Homo sapiens epidermal growth factor (EGF), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OHu94957	NM_001357021.2 Latest version!	Homo sapiens epidermal growth factor (EGF), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OHu94950	XM_017007845.1 Latest version!	Homo sapiens epidermal growth factor (EGF), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OHu94951	XM_017007846.1 Latest version!	Homo sapiens epidermal growth factor (EGF), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OHu94952	XM_017007847.1 Latest version!	Homo sapiens epidermal growth factor (EGF), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OHu94953	XM_017007848.1 Latest version!	Homo sapiens epidermal growth factor (EGF), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OHu94954	XM_017007849.1 Latest version!	Homo sapiens epidermal growth factor (EGF), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OHu94955	XM_017007850.1 Latest version!	Homo sapiens epidermal growth factor (EGF), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OHu94956	XM_017007851.1 Latest version!	Homo sapiens epidermal growth factor (EGF), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OHu94957	XM_017007852.1 Latest version!	Homo sapiens epidermal growth factor (EGF), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OHu15827	NM_001178131.3 Latest version!	Homo sapiens epidermal growth factor (EGF), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$825.30 $1179.00
OHu16028	NM_001178130.3 Latest version!	Homo sapiens epidermal growth factor (EGF), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$825.30 $1179.00
OHu38745	XM_005262796.2 Latest version!	Homo sapiens epidermal growth factor (EGF), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu57444	XM_011531707.1 Latest version!	Homo sapiens epidermal growth factor (EGF), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
View Old Accession Versions >>
OHu23582	NM_001963.5	Homo sapiens epidermal growth factor (EGF), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$274.50-$384.30 $549.00
OHu16028	NM_001178130.2	Homo sapiens epidermal growth factor (EGF), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$825.30 $1179.00
OHu94957	NM_001357021.1	Homo sapiens epidermal growth factor (EGF), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OHu15827	NM_001178131.2	Homo sapiens epidermal growth factor (EGF), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$825.30 $1179.00
Hide Old Accession Versions >>

EGF ( NM_001963.6 ) cDNA ORF clone, Homo sapiens(human) -> NP_001954.2 Homo sapiens epidermal growth factor (EGF), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu23582
Clone ID Related Accession (Same CDS sequence)	NM_001963.6 , NM_001963.5
Accession Version	NM_001963.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3624bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-31
Organism	Homo sapiens(human)
Product	pro-epidermal growth factor isoform 1 preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC005509.1, BC093731.1, AC004050.1, AK299306.1 and CA437074.1. This sequence is a reference standard in the RefSeqGene project. On May 17, 2019 this sequence version replaced NM_001963.5. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X04571.1, BC093731.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968832, SAMEA1970526 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000265171.10/ ENSP00000265171.5 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGCTGCTCA CTCTTATCAT TCTGTTGCCA GTAGTTTCAA AATTTAGTTT TGTTAGTCTC 
TCAGCACCGC AGCACTGGAG CTGTCCTGAA GGTACTCTCG CAGGAAATGG GAATTCTACT 
TGTGTGGGTC CTGCACCCTT CTTAATTTTC TCCCATGGAA ATAGTATCTT TAGGATTGAC 
ACAGAAGGAA CCAATTATGA GCAATTGGTG GTGGATGCTG GTGTCTCAGT GATCATGGAT 
TTTCATTATA ATGAGAAAAG AATCTATTGG GTGGATTTAG AAAGACAACT TTTGCAAAGA 
GTTTTTCTGA ATGGGTCAAG GCAAGAGAGA GTATGTAATA TAGAGAAAAA TGTTTCTGGA 
ATGGCAATAA ATTGGATAAA TGAAGAAGTT ATTTGGTCAA ATCAACAGGA AGGAATCATT 
ACAGTAACAG ATATGAAAGG AAATAATTCC CACATTCTTT TAAGTGCTTT AAAATATCCT 
GCAAATGTAG CAGTTGATCC AGTAGAAAGG TTTATATTTT GGTCTTCAGA GGTGGCTGGA 
AGCCTTTATA GAGCAGATCT CGATGGTGTG GGAGTGAAGG CTCTGTTGGA GACATCAGAG 
AAAATAACAG CTGTGTCATT GGATGTGCTT GATAAGCGGC TGTTTTGGAT TCAGTACAAC 
AGAGAAGGAA GCAATTCTCT TATTTGCTCC TGTGATTATG ATGGAGGTTC TGTCCACATT 
AGTAAACATC CAACACAGCA TAATTTGTTT GCAATGTCCC TTTTTGGTGA CCGTATCTTC 
TATTCAACAT GGAAAATGAA GACAATTTGG ATAGCCAACA AACACACTGG AAAGGACATG 
GTTAGAATTA ACCTCCATTC ATCATTTGTA CCACTTGGTG AACTGAAAGT AGTGCATCCA 
CTTGCACAAC CCAAGGCAGA AGATGACACT TGGGAGCCTG AGCAGAAACT TTGCAAATTG 
AGGAAAGGAA ACTGCAGCAG CACTGTGTGT GGGCAAGACC TCCAGTCACA CTTGTGCATG 
TGTGCAGAGG GATACGCCCT AAGTCGAGAC CGGAAGTACT GTGAAGATGT TAATGAATGT 
GCTTTTTGGA ATCATGGCTG TACTCTTGGG TGTAAAAACA CCCCTGGATC CTATTACTGC 
ACGTGCCCTG TAGGATTTGT TCTGCTTCCT GATGGGAAAC GATGTCATCA ACTTGTTTCC 
TGTCCACGCA ATGTGTCTGA ATGCAGCCAT GACTGTGTTC TGACATCAGA AGGTCCCTTA 
TGTTTCTGTC CTGAAGGCTC AGTGCTTGAG AGAGATGGGA AAACATGTAG CGGTTGTTCC 
TCACCCGATA ATGGTGGATG TAGCCAGCTC TGCGTTCCTC TTAGCCCAGT ATCCTGGGAA 
TGTGATTGCT TTCCTGGGTA TGACCTACAA CTGGATGAAA AAAGCTGTGC AGCTTCAGGA 
CCACAACCAT TTTTGCTGTT TGCCAATTCT CAAGATATTC GACACATGCA TTTTGATGGA 
ACAGACTATG GAACTCTGCT CAGCCAGCAG ATGGGAATGG TTTATGCCCT AGATCATGAC 
CCTGTGGAAA ATAAGATATA CTTTGCCCAT ACAGCCCTGA AGTGGATAGA GAGAGCTAAT 
ATGGATGGTT CCCAGCGAGA AAGGCTTATT GAGGAAGGAG TAGATGTGCC AGAAGGTCTT 
GCTGTGGACT GGATTGGCCG TAGATTCTAT TGGACAGACA GAGGGAAATC TCTGATTGGA 
AGGAGTGATT TAAATGGGAA ACGTTCCAAA ATAATCACTA AGGAGAACAT CTCTCAACCA 
CGAGGAATTG CTGTTCATCC AATGGCCAAG AGATTATTCT GGACTGATAC AGGGATTAAT 
CCACGAATTG AAAGTTCTTC CCTCCAAGGC CTTGGCCGTC TGGTTATAGC CAGCTCTGAT 
CTAATCTGGC CCAGTGGAAT AACGATTGAC TTCTTAACTG ACAAGTTGTA CTGGTGCGAT 
GCCAAGCAGT CTGTGATTGA AATGGCCAAT CTGGATGGTT CAAAACGCCG AAGACTTACC 
CAGAATGATG TAGGTCACCC ATTTGCTGTA GCAGTGTTTG AGGATTATGT GTGGTTCTCA 
GATTGGGCTA TGCCATCAGT AATGAGAGTA AACAAGAGGA CTGGCAAAGA TAGAGTACGT 
CTCCAAGGCA GCATGCTGAA GCCCTCATCA CTGGTTGTGG TTCATCCATT GGCAAAACCA 
GGAGCAGATC CCTGCTTATA TCAAAACGGA GGCTGTGAAC ATATTTGCAA AAAGAGGCTT 
GGAACTGCTT GGTGTTCGTG TCGTGAAGGT TTTATGAAAG CCTCAGATGG GAAAACGTGT 
CTGGCTCTGG ATGGTCATCA GCTGTTGGCA GGTGGTGAAG TTGATCTAAA GAACCAAGTA 
ACACCATTGG ACATCTTGTC CAAGACTAGA GTGTCAGAAG ATAACATTAC AGAATCTCAA 
CACATGCTAG TGGCTGAAAT CATGGTGTCA GATCAAGATG ACTGTGCTCC TGTGGGATGC 
AGCATGTATG CTCGGTGTAT TTCAGAGGGA GAGGATGCCA CATGTCAGTG TTTGAAAGGA 
TTTGCTGGGG ATGGAAAACT ATGTTCTGAT ATAGATGAAT GTGAGATGGG TGTCCCAGTG 
TGCCCCCCTG CCTCCTCCAA GTGCATCAAC ACCGAAGGTG GTTATGTCTG CCGGTGCTCA 
GAAGGCTACC AAGGAGATGG GATTCACTGT CTTGATATTG ATGAGTGCCA ACTGGGGGAG 
CACAGCTGTG GAGAGAATGC CAGCTGCACA AATACAGAGG GAGGCTATAC CTGCATGTGT 
GCTGGACGCC TGTCTGAACC AGGACTGATT TGCCCTGACT CTACTCCACC CCCTCACCTC 
AGGGAAGATG ACCACCACTA TTCCGTAAGA AATAGTGACT CTGAATGTCC CCTGTCCCAC 
GATGGGTACT GCCTCCATGA TGGTGTGTGC ATGTATATTG AAGCATTGGA CAAGTATGCA 
TGCAACTGTG TTGTTGGCTA CATCGGGGAG CGATGTCAGT ACCGAGACCT GAAGTGGTGG 
GAACTGCGCC ACGCTGGCCA CGGGCAGCAG CAGAAGGTCA TCGTGGTGGC TGTCTGCGTG 
GTGGTGCTTG TCATGCTGCT CCTCCTGAGC CTGTGGGGGG CCCACTACTA CAGGACTCAG 
AAGCTGCTAT CGAAAAACCC AAAGAATCCT TATGAGGAGT CGAGCAGAGA TGTGAGGAGT 
CGCAGGCCTG CTGACACTGA GGATGGGATG TCCTCTTGCC CTCAACCTTG GTTTGTGGTT 
ATAAAAGAAC ACCAAGACCT CAAGAATGGG GGTCAACCAG TGGCTGGTGA GGATGGCCAG 
GCAGCAGATG GGTCAATGCA ACCAACTTCA TGGAGGCAGG AGCCCCAGTT ATGTGGAATG 
GGCACAGAGC AAGGCTGCTG GATTCCAGTA TCCAGTGATA AGGGCTCCTG TCCCCAGGTA 
ATGGAGCGAA GCTTTCATAT GCCCTCCTAT GGGACACAGA CCCTTGAAGG GGGTGTCGAG 
AAGCCCCATT CTCTCCTATC AGCTAACCCA TTATGGCAAC AAAGGGCCCT GGACCCACCA 
CACCAAATGG AGCTGACTCA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001954.2
CDS	454..4077
Translation	MLLTLIILLPVVSKFSFVSLSAPQHWSCPEGTLAGNGNSTCVGPAPFLIFSHGNSIFRIDTEGTNYEQLVVDAGVSVIMDFHYNEKRIYWVDLERQLLQRVFLNGSRQERVCNIEKNVSGMAINWINEEVIWSNQQEGIITVTDMKGNNSHILLSALKYPANVAVDPVERFIFWSSEVAGSLYRADLDGVGVKALLETSEKITAVSLDVLDKRLFWIQYNREGSNSLICSCDYDGGSVHISKHPTQHNLFAMSLFGDRIFYSTWKMKTIWIANKHTGKDMVRINLHSSFVPLGELKVVHPLAQPKAEDDTWEPEQKLCKLRKGNCSSTVCGQDLQSHLCMCAEGYALSRDRKYCEDVNECAFWNHGCTLGCKNTPGSYYCTCPVGFVLLPDGKRCHQLVSCPRNVSECSHDCVLTSEGPLCFCPEGSVLERDGKTCSGCSSPDNGGCSQLCVPLSPVSWECDCFPGYDLQLDEKSCAASGPQPFLLFANSQDIRHMHFDGTDYGTLLSQQMGMVYALDHDPVENKIYFAHTALKWIERANMDGSQRERLIEEGVDVPEGLAVDWIGRRFYWTDRGKSLIGRSDLNGKRSKIITKENISQPRGIAVHPMAKRLFWTDTGINPRIESSSLQGLGRLVIASSDLIWPSGITIDFLTDKLYWCDAKQSVIEMANLDGSKRRRLTQNDVGHPFAVAVFEDYVWFSDWAMPSVMRVNKRTGKDRVRLQGSMLKPSSLVVVHPLAKPGADPCLYQNGGCEHICKKRLGTAWCSCREGFMKASDGKTCLALDGHQLLAGGEVDLKNQVTPLDILSKTRVSEDNITESQHMLVAEIMVSDQDDCAPVGCSMYARCISEGEDATCQCLKGFAGDGKLCSDIDECEMGVPVCPPASSKCINTEGGYVCRCSEGYQGDGIHCLDIDECQLGEHSCGENASCTNTEGGYTCMCAGRLSEPGLICPDSTPPPHLREDDHHYSVRNSDSECPLSHDGYCLHDGVCMYIEALDKYACNCVVGYIGERCQYRDLKWWELRHAGHGQQQKVIVVAVCVVVLVMLLLLSLWGAHYYRTQKLLSKNPKNPYEESSRDVRSRRPADTEDGMSSCPQPWFVVIKEHQDLKNGGQPVAGEDGQAADGSMQPTSWRQEPQLCGMGTEQGCWIPVSSDKGSCPQVMERSFHMPSYGTQTLEGGVEKPHSLLSANPLWQQRALDPPHQMELTQ

Target ORF information:

RefSeq Version	NM_001963.6
Organism	Homo sapiens(human)
Definition	Homo sapiens epidermal growth factor (EGF), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001963.6

ORF Insert Sequence:

ATGCTGCTCA CTCTTATCAT TCTGTTGCCA GTAGTTTCAA AATTTAGTTT TGTTAGTCTC 
TCAGCACCGC AGCACTGGAG CTGTCCTGAA GGTACTCTCG CAGGAAATGG GAATTCTACT 
TGTGTGGGTC CTGCACCCTT CTTAATTTTC TCCCATGGAA ATAGTATCTT TAGGATTGAC 
ACAGAAGGAA CCAATTATGA GCAATTGGTG GTGGATGCTG GTGTCTCAGT GATCATGGAT 
TTTCATTATA ATGAGAAAAG AATCTATTGG GTGGATTTAG AAAGACAACT TTTGCAAAGA 
GTTTTTCTGA ATGGGTCAAG GCAAGAGAGA GTATGTAATA TAGAGAAAAA TGTTTCTGGA 
ATGGCAATAA ATTGGATAAA TGAAGAAGTT ATTTGGTCAA ATCAACAGGA AGGAATCATT 
ACAGTAACAG ATATGAAAGG AAATAATTCC CACATTCTTT TAAGTGCTTT AAAATATCCT 
GCAAATGTAG CAGTTGATCC AGTAGAAAGG TTTATATTTT GGTCTTCAGA GGTGGCTGGA 
AGCCTTTATA GAGCAGATCT CGATGGTGTG GGAGTGAAGG CTCTGTTGGA GACATCAGAG 
AAAATAACAG CTGTGTCATT GGATGTGCTT GATAAGCGGC TGTTTTGGAT TCAGTACAAC 
AGAGAAGGAA GCAATTCTCT TATTTGCTCC TGTGATTATG ATGGAGGTTC TGTCCACATT 
AGTAAACATC CAACACAGCA TAATTTGTTT GCAATGTCCC TTTTTGGTGA CCGTATCTTC 
TATTCAACAT GGAAAATGAA GACAATTTGG ATAGCCAACA AACACACTGG AAAGGACATG 
GTTAGAATTA ACCTCCATTC ATCATTTGTA CCACTTGGTG AACTGAAAGT AGTGCATCCA 
CTTGCACAAC CCAAGGCAGA AGATGACACT TGGGAGCCTG AGCAGAAACT TTGCAAATTG 
AGGAAAGGAA ACTGCAGCAG CACTGTGTGT GGGCAAGACC TCCAGTCACA CTTGTGCATG 
TGTGCAGAGG GATACGCCCT AAGTCGAGAC CGGAAGTACT GTGAAGATGT TAATGAATGT 
GCTTTTTGGA ATCATGGCTG TACTCTTGGG TGTAAAAACA CCCCTGGATC CTATTACTGC 
ACGTGCCCTG TAGGATTTGT TCTGCTTCCT GATGGGAAAC GATGTCATCA ACTTGTTTCC 
TGTCCACGCA ATGTGTCTGA ATGCAGCCAT GACTGTGTTC TGACATCAGA AGGTCCCTTA 
TGTTTCTGTC CTGAAGGCTC AGTGCTTGAG AGAGATGGGA AAACATGTAG CGGTTGTTCC 
TCACCCGATA ATGGTGGATG TAGCCAGCTC TGCGTTCCTC TTAGCCCAGT ATCCTGGGAA 
TGTGATTGCT TTCCTGGGTA TGACCTACAA CTGGATGAAA AAAGCTGTGC AGCTTCAGGA 
CCACAACCAT TTTTGCTGTT TGCCAATTCT CAAGATATTC GACACATGCA TTTTGATGGA 
ACAGACTATG GAACTCTGCT CAGCCAGCAG ATGGGAATGG TTTATGCCCT AGATCATGAC 
CCTGTGGAAA ATAAGATATA CTTTGCCCAT ACAGCCCTGA AGTGGATAGA GAGAGCTAAT 
ATGGATGGTT CCCAGCGAGA AAGGCTTATT GAGGAAGGAG TAGATGTGCC AGAAGGTCTT 
GCTGTGGACT GGATTGGCCG TAGATTCTAT TGGACAGACA GAGGGAAATC TCTGATTGGA 
AGGAGTGATT TAAATGGGAA ACGTTCCAAA ATAATCACTA AGGAGAACAT CTCTCAACCA 
CGAGGAATTG CTGTTCATCC AATGGCCAAG AGATTATTCT GGACTGATAC AGGGATTAAT 
CCACGAATTG AAAGTTCTTC CCTCCAAGGC CTTGGCCGTC TGGTTATAGC CAGCTCTGAT 
CTAATCTGGC CCAGTGGAAT AACGATTGAC TTCTTAACTG ACAAGTTGTA CTGGTGCGAT 
GCCAAGCAGT CTGTGATTGA AATGGCCAAT CTGGATGGTT CAAAACGCCG AAGACTTACC 
CAGAATGATG TAGGTCACCC ATTTGCTGTA GCAGTGTTTG AGGATTATGT GTGGTTCTCA 
GATTGGGCTA TGCCATCAGT AATGAGAGTA AACAAGAGGA CTGGCAAAGA TAGAGTACGT 
CTCCAAGGCA GCATGCTGAA GCCCTCATCA CTGGTTGTGG TTCATCCATT GGCAAAACCA 
GGAGCAGATC CCTGCTTATA TCAAAACGGA GGCTGTGAAC ATATTTGCAA AAAGAGGCTT 
GGAACTGCTT GGTGTTCGTG TCGTGAAGGT TTTATGAAAG CCTCAGATGG GAAAACGTGT 
CTGGCTCTGG ATGGTCATCA GCTGTTGGCA GGTGGTGAAG TTGATCTAAA GAACCAAGTA 
ACACCATTGG ACATCTTGTC CAAGACTAGA GTGTCAGAAG ATAACATTAC AGAATCTCAA 
CACATGCTAG TGGCTGAAAT CATGGTGTCA GATCAAGATG ACTGTGCTCC TGTGGGATGC 
AGCATGTATG CTCGGTGTAT TTCAGAGGGA GAGGATGCCA CATGTCAGTG TTTGAAAGGA 
TTTGCTGGGG ATGGAAAACT ATGTTCTGAT ATAGATGAAT GTGAGATGGG TGTCCCAGTG 
TGCCCCCCTG CCTCCTCCAA GTGCATCAAC ACCGAAGGTG GTTATGTCTG CCGGTGCTCA 
GAAGGCTACC AAGGAGATGG GATTCACTGT CTTGATATTG ATGAGTGCCA ACTGGGGGAG 
CACAGCTGTG GAGAGAATGC CAGCTGCACA AATACAGAGG GAGGCTATAC CTGCATGTGT 
GCTGGACGCC TGTCTGAACC AGGACTGATT TGCCCTGACT CTACTCCACC CCCTCACCTC 
AGGGAAGATG ACCACCACTA TTCCGTAAGA AATAGTGACT CTGAATGTCC CCTGTCCCAC 
GATGGGTACT GCCTCCATGA TGGTGTGTGC ATGTATATTG AAGCATTGGA CAAGTATGCA 
TGCAACTGTG TTGTTGGCTA CATCGGGGAG CGATGTCAGT ACCGAGACCT GAAGTGGTGG 
GAACTGCGCC ACGCTGGCCA CGGGCAGCAG CAGAAGGTCA TCGTGGTGGC TGTCTGCGTG 
GTGGTGCTTG TCATGCTGCT CCTCCTGAGC CTGTGGGGGG CCCACTACTA CAGGACTCAG 
AAGCTGCTAT CGAAAAACCC AAAGAATCCT TATGAGGAGT CGAGCAGAGA TGTGAGGAGT 
CGCAGGCCTG CTGACACTGA GGATGGGATG TCCTCTTGCC CTCAACCTTG GTTTGTGGTT 
ATAAAAGAAC ACCAAGACCT CAAGAATGGG GGTCAACCAG TGGCTGGTGA GGATGGCCAG 
GCAGCAGATG GGTCAATGCA ACCAACTTCA TGGAGGCAGG AGCCCCAGTT ATGTGGAATG 
GGCACAGAGC AAGGCTGCTG GATTCCAGTA TCCAGTGATA AGGGCTCCTG TCCCCAGGTA 
ATGGAGCGAA GCTTTCATAT GCCCTCCTAT GGGACACAGA CCCTTGAAGG GGGTGTCGAG 
AAGCCCCATT CTCTCCTATC AGCTAACCCA TTATGGCAAC AAAGGGCCCT GGACCCACCA 
CACCAAATGG AGCTGACTCA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EGF ( NM_001963.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_001954.2 Homo sapiens epidermal growth factor (EGF), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu23582
Clone ID Related Accession (Same CDS sequence)	NM_001963.6 , NM_001963.5
Accession Version	NM_001963.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3624bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-24
Organism	Homo sapiens(human)
Product	pro-epidermal growth factor isoform 1 preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC005509.1, BC093731.1, AC004050.1, AK299306.1 and CA437074.1. This sequence is a reference standard in the RefSeqGene project. On Jan 8, 2016 this sequence version replaced NM_001963.4. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X04571.1, BC093731.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968832, SAMEA1970526 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

ATGCTGCTCA CTCTTATCAT TCTGTTGCCA GTAGTTTCAA AATTTAGTTT TGTTAGTCTC 
TCAGCACCGC AGCACTGGAG CTGTCCTGAA GGTACTCTCG CAGGAAATGG GAATTCTACT 
TGTGTGGGTC CTGCACCCTT CTTAATTTTC TCCCATGGAA ATAGTATCTT TAGGATTGAC 
ACAGAAGGAA CCAATTATGA GCAATTGGTG GTGGATGCTG GTGTCTCAGT GATCATGGAT 
TTTCATTATA ATGAGAAAAG AATCTATTGG GTGGATTTAG AAAGACAACT TTTGCAAAGA 
GTTTTTCTGA ATGGGTCAAG GCAAGAGAGA GTATGTAATA TAGAGAAAAA TGTTTCTGGA 
ATGGCAATAA ATTGGATAAA TGAAGAAGTT ATTTGGTCAA ATCAACAGGA AGGAATCATT 
ACAGTAACAG ATATGAAAGG AAATAATTCC CACATTCTTT TAAGTGCTTT AAAATATCCT 
GCAAATGTAG CAGTTGATCC AGTAGAAAGG TTTATATTTT GGTCTTCAGA GGTGGCTGGA 
AGCCTTTATA GAGCAGATCT CGATGGTGTG GGAGTGAAGG CTCTGTTGGA GACATCAGAG 
AAAATAACAG CTGTGTCATT GGATGTGCTT GATAAGCGGC TGTTTTGGAT TCAGTACAAC 
AGAGAAGGAA GCAATTCTCT TATTTGCTCC TGTGATTATG ATGGAGGTTC TGTCCACATT 
AGTAAACATC CAACACAGCA TAATTTGTTT GCAATGTCCC TTTTTGGTGA CCGTATCTTC 
TATTCAACAT GGAAAATGAA GACAATTTGG ATAGCCAACA AACACACTGG AAAGGACATG 
GTTAGAATTA ACCTCCATTC ATCATTTGTA CCACTTGGTG AACTGAAAGT AGTGCATCCA 
CTTGCACAAC CCAAGGCAGA AGATGACACT TGGGAGCCTG AGCAGAAACT TTGCAAATTG 
AGGAAAGGAA ACTGCAGCAG CACTGTGTGT GGGCAAGACC TCCAGTCACA CTTGTGCATG 
TGTGCAGAGG GATACGCCCT AAGTCGAGAC CGGAAGTACT GTGAAGATGT TAATGAATGT 
GCTTTTTGGA ATCATGGCTG TACTCTTGGG TGTAAAAACA CCCCTGGATC CTATTACTGC 
ACGTGCCCTG TAGGATTTGT TCTGCTTCCT GATGGGAAAC GATGTCATCA ACTTGTTTCC 
TGTCCACGCA ATGTGTCTGA ATGCAGCCAT GACTGTGTTC TGACATCAGA AGGTCCCTTA 
TGTTTCTGTC CTGAAGGCTC AGTGCTTGAG AGAGATGGGA AAACATGTAG CGGTTGTTCC 
TCACCCGATA ATGGTGGATG TAGCCAGCTC TGCGTTCCTC TTAGCCCAGT ATCCTGGGAA 
TGTGATTGCT TTCCTGGGTA TGACCTACAA CTGGATGAAA AAAGCTGTGC AGCTTCAGGA 
CCACAACCAT TTTTGCTGTT TGCCAATTCT CAAGATATTC GACACATGCA TTTTGATGGA 
ACAGACTATG GAACTCTGCT CAGCCAGCAG ATGGGAATGG TTTATGCCCT AGATCATGAC 
CCTGTGGAAA ATAAGATATA CTTTGCCCAT ACAGCCCTGA AGTGGATAGA GAGAGCTAAT 
ATGGATGGTT CCCAGCGAGA AAGGCTTATT GAGGAAGGAG TAGATGTGCC AGAAGGTCTT 
GCTGTGGACT GGATTGGCCG TAGATTCTAT TGGACAGACA GAGGGAAATC TCTGATTGGA 
AGGAGTGATT TAAATGGGAA ACGTTCCAAA ATAATCACTA AGGAGAACAT CTCTCAACCA 
CGAGGAATTG CTGTTCATCC AATGGCCAAG AGATTATTCT GGACTGATAC AGGGATTAAT 
CCACGAATTG AAAGTTCTTC CCTCCAAGGC CTTGGCCGTC TGGTTATAGC CAGCTCTGAT 
CTAATCTGGC CCAGTGGAAT AACGATTGAC TTCTTAACTG ACAAGTTGTA CTGGTGCGAT 
GCCAAGCAGT CTGTGATTGA AATGGCCAAT CTGGATGGTT CAAAACGCCG AAGACTTACC 
CAGAATGATG TAGGTCACCC ATTTGCTGTA GCAGTGTTTG AGGATTATGT GTGGTTCTCA 
GATTGGGCTA TGCCATCAGT AATGAGAGTA AACAAGAGGA CTGGCAAAGA TAGAGTACGT 
CTCCAAGGCA GCATGCTGAA GCCCTCATCA CTGGTTGTGG TTCATCCATT GGCAAAACCA 
GGAGCAGATC CCTGCTTATA TCAAAACGGA GGCTGTGAAC ATATTTGCAA AAAGAGGCTT 
GGAACTGCTT GGTGTTCGTG TCGTGAAGGT TTTATGAAAG CCTCAGATGG GAAAACGTGT 
CTGGCTCTGG ATGGTCATCA GCTGTTGGCA GGTGGTGAAG TTGATCTAAA GAACCAAGTA 
ACACCATTGG ACATCTTGTC CAAGACTAGA GTGTCAGAAG ATAACATTAC AGAATCTCAA 
CACATGCTAG TGGCTGAAAT CATGGTGTCA GATCAAGATG ACTGTGCTCC TGTGGGATGC 
AGCATGTATG CTCGGTGTAT TTCAGAGGGA GAGGATGCCA CATGTCAGTG TTTGAAAGGA 
TTTGCTGGGG ATGGAAAACT ATGTTCTGAT ATAGATGAAT GTGAGATGGG TGTCCCAGTG 
TGCCCCCCTG CCTCCTCCAA GTGCATCAAC ACCGAAGGTG GTTATGTCTG CCGGTGCTCA 
GAAGGCTACC AAGGAGATGG GATTCACTGT CTTGATATTG ATGAGTGCCA ACTGGGGGAG 
CACAGCTGTG GAGAGAATGC CAGCTGCACA AATACAGAGG GAGGCTATAC CTGCATGTGT 
GCTGGACGCC TGTCTGAACC AGGACTGATT TGCCCTGACT CTACTCCACC CCCTCACCTC 
AGGGAAGATG ACCACCACTA TTCCGTAAGA AATAGTGACT CTGAATGTCC CCTGTCCCAC 
GATGGGTACT GCCTCCATGA TGGTGTGTGC ATGTATATTG AAGCATTGGA CAAGTATGCA 
TGCAACTGTG TTGTTGGCTA CATCGGGGAG CGATGTCAGT ACCGAGACCT GAAGTGGTGG 
GAACTGCGCC ACGCTGGCCA CGGGCAGCAG CAGAAGGTCA TCGTGGTGGC TGTCTGCGTG 
GTGGTGCTTG TCATGCTGCT CCTCCTGAGC CTGTGGGGGG CCCACTACTA CAGGACTCAG 
AAGCTGCTAT CGAAAAACCC AAAGAATCCT TATGAGGAGT CGAGCAGAGA TGTGAGGAGT 
CGCAGGCCTG CTGACACTGA GGATGGGATG TCCTCTTGCC CTCAACCTTG GTTTGTGGTT 
ATAAAAGAAC ACCAAGACCT CAAGAATGGG GGTCAACCAG TGGCTGGTGA GGATGGCCAG 
GCAGCAGATG GGTCAATGCA ACCAACTTCA TGGAGGCAGG AGCCCCAGTT ATGTGGAATG 
GGCACAGAGC AAGGCTGCTG GATTCCAGTA TCCAGTGATA AGGGCTCCTG TCCCCAGGTA 
ATGGAGCGAA GCTTTCATAT GCCCTCCTAT GGGACACAGA CCCTTGAAGG GGGTGTCGAG 
AAGCCCCATT CTCTCCTATC AGCTAACCCA TTATGGCAAC AAAGGGCCCT GGACCCACCA 
CACCAAATGG AGCTGACTCA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001954.2
CDS	453..4076
Translation	MLLTLIILLPVVSKFSFVSLSAPQHWSCPEGTLAGNGNSTCVGPAPFLIFSHGNSIFRIDTEGTNYEQLVVDAGVSVIMDFHYNEKRIYWVDLERQLLQRVFLNGSRQERVCNIEKNVSGMAINWINEEVIWSNQQEGIITVTDMKGNNSHILLSALKYPANVAVDPVERFIFWSSEVAGSLYRADLDGVGVKALLETSEKITAVSLDVLDKRLFWIQYNREGSNSLICSCDYDGGSVHISKHPTQHNLFAMSLFGDRIFYSTWKMKTIWIANKHTGKDMVRINLHSSFVPLGELKVVHPLAQPKAEDDTWEPEQKLCKLRKGNCSSTVCGQDLQSHLCMCAEGYALSRDRKYCEDVNECAFWNHGCTLGCKNTPGSYYCTCPVGFVLLPDGKRCHQLVSCPRNVSECSHDCVLTSEGPLCFCPEGSVLERDGKTCSGCSSPDNGGCSQLCVPLSPVSWECDCFPGYDLQLDEKSCAASGPQPFLLFANSQDIRHMHFDGTDYGTLLSQQMGMVYALDHDPVENKIYFAHTALKWIERANMDGSQRERLIEEGVDVPEGLAVDWIGRRFYWTDRGKSLIGRSDLNGKRSKIITKENISQPRGIAVHPMAKRLFWTDTGINPRIESSSLQGLGRLVIASSDLIWPSGITIDFLTDKLYWCDAKQSVIEMANLDGSKRRRLTQNDVGHPFAVAVFEDYVWFSDWAMPSVMRVNKRTGKDRVRLQGSMLKPSSLVVVHPLAKPGADPCLYQNGGCEHICKKRLGTAWCSCREGFMKASDGKTCLALDGHQLLAGGEVDLKNQVTPLDILSKTRVSEDNITESQHMLVAEIMVSDQDDCAPVGCSMYARCISEGEDATCQCLKGFAGDGKLCSDIDECEMGVPVCPPASSKCINTEGGYVCRCSEGYQGDGIHCLDIDECQLGEHSCGENASCTNTEGGYTCMCAGRLSEPGLICPDSTPPPHLREDDHHYSVRNSDSECPLSHDGYCLHDGVCMYIEALDKYACNCVVGYIGERCQYRDLKWWELRHAGHGQQQKVIVVAVCVVVLVMLLLLSLWGAHYYRTQKLLSKNPKNPYEESSRDVRSRRPADTEDGMSSCPQPWFVVIKEHQDLKNGGQPVAGEDGQAADGSMQPTSWRQEPQLCGMGTEQGCWIPVSSDKGSCPQVMERSFHMPSYGTQTLEGGVEKPHSLLSANPLWQQRALDPPHQMELTQ

Target ORF information:

RefSeq Version	NM_001963.5
Organism	Homo sapiens(human)
Definition	Homo sapiens epidermal growth factor (EGF), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001963.5

ORF Insert Sequence:

ATGCTGCTCA CTCTTATCAT TCTGTTGCCA GTAGTTTCAA AATTTAGTTT TGTTAGTCTC 
TCAGCACCGC AGCACTGGAG CTGTCCTGAA GGTACTCTCG CAGGAAATGG GAATTCTACT 
TGTGTGGGTC CTGCACCCTT CTTAATTTTC TCCCATGGAA ATAGTATCTT TAGGATTGAC 
ACAGAAGGAA CCAATTATGA GCAATTGGTG GTGGATGCTG GTGTCTCAGT GATCATGGAT 
TTTCATTATA ATGAGAAAAG AATCTATTGG GTGGATTTAG AAAGACAACT TTTGCAAAGA 
GTTTTTCTGA ATGGGTCAAG GCAAGAGAGA GTATGTAATA TAGAGAAAAA TGTTTCTGGA 
ATGGCAATAA ATTGGATAAA TGAAGAAGTT ATTTGGTCAA ATCAACAGGA AGGAATCATT 
ACAGTAACAG ATATGAAAGG AAATAATTCC CACATTCTTT TAAGTGCTTT AAAATATCCT 
GCAAATGTAG CAGTTGATCC AGTAGAAAGG TTTATATTTT GGTCTTCAGA GGTGGCTGGA 
AGCCTTTATA GAGCAGATCT CGATGGTGTG GGAGTGAAGG CTCTGTTGGA GACATCAGAG 
AAAATAACAG CTGTGTCATT GGATGTGCTT GATAAGCGGC TGTTTTGGAT TCAGTACAAC 
AGAGAAGGAA GCAATTCTCT TATTTGCTCC TGTGATTATG ATGGAGGTTC TGTCCACATT 
AGTAAACATC CAACACAGCA TAATTTGTTT GCAATGTCCC TTTTTGGTGA CCGTATCTTC 
TATTCAACAT GGAAAATGAA GACAATTTGG ATAGCCAACA AACACACTGG AAAGGACATG 
GTTAGAATTA ACCTCCATTC ATCATTTGTA CCACTTGGTG AACTGAAAGT AGTGCATCCA 
CTTGCACAAC CCAAGGCAGA AGATGACACT TGGGAGCCTG AGCAGAAACT TTGCAAATTG 
AGGAAAGGAA ACTGCAGCAG CACTGTGTGT GGGCAAGACC TCCAGTCACA CTTGTGCATG 
TGTGCAGAGG GATACGCCCT AAGTCGAGAC CGGAAGTACT GTGAAGATGT TAATGAATGT 
GCTTTTTGGA ATCATGGCTG TACTCTTGGG TGTAAAAACA CCCCTGGATC CTATTACTGC 
ACGTGCCCTG TAGGATTTGT TCTGCTTCCT GATGGGAAAC GATGTCATCA ACTTGTTTCC 
TGTCCACGCA ATGTGTCTGA ATGCAGCCAT GACTGTGTTC TGACATCAGA AGGTCCCTTA 
TGTTTCTGTC CTGAAGGCTC AGTGCTTGAG AGAGATGGGA AAACATGTAG CGGTTGTTCC 
TCACCCGATA ATGGTGGATG TAGCCAGCTC TGCGTTCCTC TTAGCCCAGT ATCCTGGGAA 
TGTGATTGCT TTCCTGGGTA TGACCTACAA CTGGATGAAA AAAGCTGTGC AGCTTCAGGA 
CCACAACCAT TTTTGCTGTT TGCCAATTCT CAAGATATTC GACACATGCA TTTTGATGGA 
ACAGACTATG GAACTCTGCT CAGCCAGCAG ATGGGAATGG TTTATGCCCT AGATCATGAC 
CCTGTGGAAA ATAAGATATA CTTTGCCCAT ACAGCCCTGA AGTGGATAGA GAGAGCTAAT 
ATGGATGGTT CCCAGCGAGA AAGGCTTATT GAGGAAGGAG TAGATGTGCC AGAAGGTCTT 
GCTGTGGACT GGATTGGCCG TAGATTCTAT TGGACAGACA GAGGGAAATC TCTGATTGGA 
AGGAGTGATT TAAATGGGAA ACGTTCCAAA ATAATCACTA AGGAGAACAT CTCTCAACCA 
CGAGGAATTG CTGTTCATCC AATGGCCAAG AGATTATTCT GGACTGATAC AGGGATTAAT 
CCACGAATTG AAAGTTCTTC CCTCCAAGGC CTTGGCCGTC TGGTTATAGC CAGCTCTGAT 
CTAATCTGGC CCAGTGGAAT AACGATTGAC TTCTTAACTG ACAAGTTGTA CTGGTGCGAT 
GCCAAGCAGT CTGTGATTGA AATGGCCAAT CTGGATGGTT CAAAACGCCG AAGACTTACC 
CAGAATGATG TAGGTCACCC ATTTGCTGTA GCAGTGTTTG AGGATTATGT GTGGTTCTCA 
GATTGGGCTA TGCCATCAGT AATGAGAGTA AACAAGAGGA CTGGCAAAGA TAGAGTACGT 
CTCCAAGGCA GCATGCTGAA GCCCTCATCA CTGGTTGTGG TTCATCCATT GGCAAAACCA 
GGAGCAGATC CCTGCTTATA TCAAAACGGA GGCTGTGAAC ATATTTGCAA AAAGAGGCTT 
GGAACTGCTT GGTGTTCGTG TCGTGAAGGT TTTATGAAAG CCTCAGATGG GAAAACGTGT 
CTGGCTCTGG ATGGTCATCA GCTGTTGGCA GGTGGTGAAG TTGATCTAAA GAACCAAGTA 
ACACCATTGG ACATCTTGTC CAAGACTAGA GTGTCAGAAG ATAACATTAC AGAATCTCAA 
CACATGCTAG TGGCTGAAAT CATGGTGTCA GATCAAGATG ACTGTGCTCC TGTGGGATGC 
AGCATGTATG CTCGGTGTAT TTCAGAGGGA GAGGATGCCA CATGTCAGTG TTTGAAAGGA 
TTTGCTGGGG ATGGAAAACT ATGTTCTGAT ATAGATGAAT GTGAGATGGG TGTCCCAGTG 
TGCCCCCCTG CCTCCTCCAA GTGCATCAAC ACCGAAGGTG GTTATGTCTG CCGGTGCTCA 
GAAGGCTACC AAGGAGATGG GATTCACTGT CTTGATATTG ATGAGTGCCA ACTGGGGGAG 
CACAGCTGTG GAGAGAATGC CAGCTGCACA AATACAGAGG GAGGCTATAC CTGCATGTGT 
GCTGGACGCC TGTCTGAACC AGGACTGATT TGCCCTGACT CTACTCCACC CCCTCACCTC 
AGGGAAGATG ACCACCACTA TTCCGTAAGA AATAGTGACT CTGAATGTCC CCTGTCCCAC 
GATGGGTACT GCCTCCATGA TGGTGTGTGC ATGTATATTG AAGCATTGGA CAAGTATGCA 
TGCAACTGTG TTGTTGGCTA CATCGGGGAG CGATGTCAGT ACCGAGACCT GAAGTGGTGG 
GAACTGCGCC ACGCTGGCCA CGGGCAGCAG CAGAAGGTCA TCGTGGTGGC TGTCTGCGTG 
GTGGTGCTTG TCATGCTGCT CCTCCTGAGC CTGTGGGGGG CCCACTACTA CAGGACTCAG 
AAGCTGCTAT CGAAAAACCC AAAGAATCCT TATGAGGAGT CGAGCAGAGA TGTGAGGAGT 
CGCAGGCCTG CTGACACTGA GGATGGGATG TCCTCTTGCC CTCAACCTTG GTTTGTGGTT 
ATAAAAGAAC ACCAAGACCT CAAGAATGGG GGTCAACCAG TGGCTGGTGA GGATGGCCAG 
GCAGCAGATG GGTCAATGCA ACCAACTTCA TGGAGGCAGG AGCCCCAGTT ATGTGGAATG 
GGCACAGAGC AAGGCTGCTG GATTCCAGTA TCCAGTGATA AGGGCTCCTG TCCCCAGGTA 
ATGGAGCGAA GCTTTCATAT GCCCTCCTAT GGGACACAGA CCCTTGAAGG GGGTGTCGAG 
AAGCCCCATT CTCTCCTATC AGCTAACCCA TTATGGCAAC AAAGGGCCCT GGACCCACCA 
CACCAAATGG AGCTGACTCA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EGF ( XM_017007853.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016863342.1 Homo sapiens epidermal growth factor (EGF), transcript variant X12, mRNA.

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CloneID	OHu94958
Clone ID Related Accession (Same CDS sequence)	XM_017007853.1
Accession Version	XM_017007853.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2805bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	pro-epidermal growth factor isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCTCA CTCTTATCAT TCTGTTGCCA GTAGTTTCAA AATTTAGTTT TGTTAGTCTC 
TCAGCACCGC AGCACTGGAG CTGTCCTGAA GGTACTCTCG CAGGAAATGG GAATTCTACT 
TGTGTGGTTT GGTCTCTTTC TTCCCACCCA GGTCCTGCAC CCTTCTTAAT TTTCTCCCAT 
GGAAATAGTA TCTTTAGGAT TGACACAGAA GGAACCAATT ATGAGCAATT GGTGGTGGAT 
GCTGGTGTCT CAGTGATCAT GGATTTTCAT TATAATGAGA AAAGAATCTA TTGGGTGGAT 
TTAGAAAGAC AACTTTTGCA AAGAGTTTTT CTGAATGGGT CAAGGCAAGA GAGAGTATGT 
AATATAGAGA AAAATGTTTC TGGAATGGCA ATAAATTGGA TAAATGAAGA AGTTATTTGG 
TCAAATCAAC AGGAAGGAAT CATTACAGTA ACAGATATGA AAGGAAATAA TTCCCACATT 
CTTTTAAGTG CTTTAAAATA TCCTGCAAAT GTAGCAGTTG ATCCAGTAGA AAGGTTTATA 
TTTTGGTCTT CAGAGGTGGC TGGAAGCCTT TATAGAGCAG ATCTCGATGG TGTGGGAGTG 
AAGGCTCTGT TGGAGACATC AGAGAAAATA ACAGCTGTGT CATTGGATGT GCTTGATAAG 
CGGCTGTTTT GGATTCAGTA CAACAGAGAA GGAAGCAATT CTCTTATTTG CTCCTGTGAT 
TATGATGGAG GTTCTGTCCA CATTAGTAAA CATCCAACAC AGCATAATTT GTTTGCAATG 
TCCCTTTTTG GTGACCGTAT CTTCTATTCA ACATGGAAAA TGAAGACAAT TTGGATAGCC 
AACAAACACA CTGGAAAGGA CATGGTTAGA ATTAACCTCC ATTCATCATT TGTACCACTT 
GGTGAACTGA AAGTAGTGCA TCCACTTGCA CAACCCAAGG CAGAAGATGA CACTTGGGAG 
CCTGAGCAGA AACTTTGCAA ATTGAGGAAA GGAAACTGCA GCAGCACTGT GTGTGGGCAA 
GACCTCCAGT CACACTTGTG CATGTGTGCA GAGGGATACG CCCTAAGTCG AGACCGGAAG 
TACTGTGAAG ATGTTAATGA ATGTGCTTTT TGGAATCATG GCTGTACTCT TGGGTGTAAA 
AACACCCCTG GATCCTATTA CTGCACGTGC CCTGTAGGAT TTGTTCTGCT TCCTGATGGG 
AAACGATGTC ATCAACTTGT TTCCTGTCCA CGCAATGTGT CTGAATGCAG CCATGACTGT 
GTTCTGACAT CAGAAGGTCC CTTATGTTTC TGTCCTGAAG GCTCAGTGCT TGAGAGAGAT 
GGGAAAACAT GTAGCGGTTG TTCCTCACCC GATAATGGTG GATGTAGCCA GCTCTGCGTT 
CCTCTTAGCC CAGTATCCTG GGAATGTGAT TGCTTTCCTG GGTATGACCT ACAACTGGAT 
GAAAAAAGCT GTGCAGCTTC AGGACCACAA CCATTTTTGC TGTTTGCCAA TTCTCAAGAT 
ATTCGACACA TGCATTTTGA TGGAACAGAC TATGGAACTC TGCTCAGCCA GCAGATGGGA 
ATGGTTTATG CCCTAGATCA TGACCCTGTG GAAAATAAGA TATACTTTGC CCATACAGCC 
CTGAAGTGGA TAGAGAGAGC TAATATGGAT GGTTCCCAGC GAGAAAGGCT TATTGAGGAA 
GGAGTAGATG TGCCAGAAGG TCTTGCTGTG GACTGGATTG GCCGTAGATT CTATTGGACA 
GACAGAGGGA AATCTCTGAT TGGAAGGAGT GATTTAAATG GGAAACGTTC CAAAATAATC 
ACTAAGGAGA ACATCTCTCA ACCACGAGGA ATTGCTGTTC ATCCAATGGC CAAGAGATTA 
TTCTGGACTG ATACAGGGAT TAATCCACGA ATTGAAAGTT CTTCCCTCCA AGGCCTTGGC 
CGTCTGGTTA TAGCCAGCTC TGATCTAATC TGGCCCAGTG GAATAACGAT TGACTTCTTA 
ACTGACAAGT TGTACTGGTG CGATGCCAAG CAGTCTGTGA TTGAAATGGC CAATCTGGAT 
GGTTCAAAAC GCCGAAGACT TACCCAGAAT GATGTAGGTC ACCCATTTGC TGTAGCAGTG 
TTTGAGGATT ATGTGTGGTT CTCAGATTGG GCTATGCCAT CAGTAATGAG AGTAAACAAG 
AGGACTGGCA AAGATAGAGT ACGTCTCCAA GGCAGCATGC TGAAGCCCTC ATCACTGGTT 
GTGGTTCATC CATTGGCAAA ACCAGGAGCA GATCCCTGCT TATATCAAAA CGGAGGCTGT 
GAACATATTT GCAAAAAGAG GCTTGGAACT GCTTGGTGTT CGTGTCGTGA AGGTTTTATG 
AAAGCCTCAG ATGGGAAAAC GTGTCTGGCT CTGGATGGTC ATCAGCTGTT GGCAGGTGGT 
GAAGTTGATC TAAAGAACCA AGTAACACCA TTGGACATCT TGTCCAAGAC TAGAGTGTCA 
GAAGATAACA TTACAGAATC TCAACACATG CTAGTGGCTG AAATCATGGT GTCAGATCAA 
GATGACTGTG CTCCTGTGGG ATGCAGCATG TATGCTCGGT GTATTTCAGA GGGAGAGGAT 
GCCACATGTC AGTGTTTGAA AGGATTTGCT GGGGATGGAA AACTATGTTC TGATATAGAT 
GAATGTGAGA TGGGTGTCCC AGTGTGCCCC CCTGCCTCCT CCAAGTGCAT CAACACCGAA 
GGTGGTTATG TCTGCCGGTG CTCAGAAGGC TACCAAGGAG ATGGGATTCA CTGTCTTGCT 
GTGTTGTTGG CTACATCGGG GAGCGATGTC AGTACCGAGA CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016863342.1
CDS	454..3258
Translation	MLLTLIILLPVVSKFSFVSLSAPQHWSCPEGTLAGNGNSTCVVWSLSSHPGPAPFLIFSHGNSIFRIDTEGTNYEQLVVDAGVSVIMDFHYNEKRIYWVDLERQLLQRVFLNGSRQERVCNIEKNVSGMAINWINEEVIWSNQQEGIITVTDMKGNNSHILLSALKYPANVAVDPVERFIFWSSEVAGSLYRADLDGVGVKALLETSEKITAVSLDVLDKRLFWIQYNREGSNSLICSCDYDGGSVHISKHPTQHNLFAMSLFGDRIFYSTWKMKTIWIANKHTGKDMVRINLHSSFVPLGELKVVHPLAQPKAEDDTWEPEQKLCKLRKGNCSSTVCGQDLQSHLCMCAEGYALSRDRKYCEDVNECAFWNHGCTLGCKNTPGSYYCTCPVGFVLLPDGKRCHQLVSCPRNVSECSHDCVLTSEGPLCFCPEGSVLERDGKTCSGCSSPDNGGCSQLCVPLSPVSWECDCFPGYDLQLDEKSCAASGPQPFLLFANSQDIRHMHFDGTDYGTLLSQQMGMVYALDHDPVENKIYFAHTALKWIERANMDGSQRERLIEEGVDVPEGLAVDWIGRRFYWTDRGKSLIGRSDLNGKRSKIITKENISQPRGIAVHPMAKRLFWTDTGINPRIESSSLQGLGRLVIASSDLIWPSGITIDFLTDKLYWCDAKQSVIEMANLDGSKRRRLTQNDVGHPFAVAVFEDYVWFSDWAMPSVMRVNKRTGKDRVRLQGSMLKPSSLVVVHPLAKPGADPCLYQNGGCEHICKKRLGTAWCSCREGFMKASDGKTCLALDGHQLLAGGEVDLKNQVTPLDILSKTRVSEDNITESQHMLVAEIMVSDQDDCAPVGCSMYARCISEGEDATCQCLKGFAGDGKLCSDIDECEMGVPVCPPASSKCINTEGGYVCRCSEGYQGDGIHCLAVLLATSGSDVSTET

Target ORF information:

RefSeq Version	XM_017007853.1
Organism	Homo sapiens(human)
Definition	Homo sapiens epidermal growth factor (EGF), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017007853.1

ORF Insert Sequence:

ATGCTGCTCA CTCTTATCAT TCTGTTGCCA GTAGTTTCAA AATTTAGTTT TGTTAGTCTC 
TCAGCACCGC AGCACTGGAG CTGTCCTGAA GGTACTCTCG CAGGAAATGG GAATTCTACT 
TGTGTGGTTT GGTCTCTTTC TTCCCACCCA GGTCCTGCAC CCTTCTTAAT TTTCTCCCAT 
GGAAATAGTA TCTTTAGGAT TGACACAGAA GGAACCAATT ATGAGCAATT GGTGGTGGAT 
GCTGGTGTCT CAGTGATCAT GGATTTTCAT TATAATGAGA AAAGAATCTA TTGGGTGGAT 
TTAGAAAGAC AACTTTTGCA AAGAGTTTTT CTGAATGGGT CAAGGCAAGA GAGAGTATGT 
AATATAGAGA AAAATGTTTC TGGAATGGCA ATAAATTGGA TAAATGAAGA AGTTATTTGG 
TCAAATCAAC AGGAAGGAAT CATTACAGTA ACAGATATGA AAGGAAATAA TTCCCACATT 
CTTTTAAGTG CTTTAAAATA TCCTGCAAAT GTAGCAGTTG ATCCAGTAGA AAGGTTTATA 
TTTTGGTCTT CAGAGGTGGC TGGAAGCCTT TATAGAGCAG ATCTCGATGG TGTGGGAGTG 
AAGGCTCTGT TGGAGACATC AGAGAAAATA ACAGCTGTGT CATTGGATGT GCTTGATAAG 
CGGCTGTTTT GGATTCAGTA CAACAGAGAA GGAAGCAATT CTCTTATTTG CTCCTGTGAT 
TATGATGGAG GTTCTGTCCA CATTAGTAAA CATCCAACAC AGCATAATTT GTTTGCAATG 
TCCCTTTTTG GTGACCGTAT CTTCTATTCA ACATGGAAAA TGAAGACAAT TTGGATAGCC 
AACAAACACA CTGGAAAGGA CATGGTTAGA ATTAACCTCC ATTCATCATT TGTACCACTT 
GGTGAACTGA AAGTAGTGCA TCCACTTGCA CAACCCAAGG CAGAAGATGA CACTTGGGAG 
CCTGAGCAGA AACTTTGCAA ATTGAGGAAA GGAAACTGCA GCAGCACTGT GTGTGGGCAA 
GACCTCCAGT CACACTTGTG CATGTGTGCA GAGGGATACG CCCTAAGTCG AGACCGGAAG 
TACTGTGAAG ATGTTAATGA ATGTGCTTTT TGGAATCATG GCTGTACTCT TGGGTGTAAA 
AACACCCCTG GATCCTATTA CTGCACGTGC CCTGTAGGAT TTGTTCTGCT TCCTGATGGG 
AAACGATGTC ATCAACTTGT TTCCTGTCCA CGCAATGTGT CTGAATGCAG CCATGACTGT 
GTTCTGACAT CAGAAGGTCC CTTATGTTTC TGTCCTGAAG GCTCAGTGCT TGAGAGAGAT 
GGGAAAACAT GTAGCGGTTG TTCCTCACCC GATAATGGTG GATGTAGCCA GCTCTGCGTT 
CCTCTTAGCC CAGTATCCTG GGAATGTGAT TGCTTTCCTG GGTATGACCT ACAACTGGAT 
GAAAAAAGCT GTGCAGCTTC AGGACCACAA CCATTTTTGC TGTTTGCCAA TTCTCAAGAT 
ATTCGACACA TGCATTTTGA TGGAACAGAC TATGGAACTC TGCTCAGCCA GCAGATGGGA 
ATGGTTTATG CCCTAGATCA TGACCCTGTG GAAAATAAGA TATACTTTGC CCATACAGCC 
CTGAAGTGGA TAGAGAGAGC TAATATGGAT GGTTCCCAGC GAGAAAGGCT TATTGAGGAA 
GGAGTAGATG TGCCAGAAGG TCTTGCTGTG GACTGGATTG GCCGTAGATT CTATTGGACA 
GACAGAGGGA AATCTCTGAT TGGAAGGAGT GATTTAAATG GGAAACGTTC CAAAATAATC 
ACTAAGGAGA ACATCTCTCA ACCACGAGGA ATTGCTGTTC ATCCAATGGC CAAGAGATTA 
TTCTGGACTG ATACAGGGAT TAATCCACGA ATTGAAAGTT CTTCCCTCCA AGGCCTTGGC 
CGTCTGGTTA TAGCCAGCTC TGATCTAATC TGGCCCAGTG GAATAACGAT TGACTTCTTA 
ACTGACAAGT TGTACTGGTG CGATGCCAAG CAGTCTGTGA TTGAAATGGC CAATCTGGAT 
GGTTCAAAAC GCCGAAGACT TACCCAGAAT GATGTAGGTC ACCCATTTGC TGTAGCAGTG 
TTTGAGGATT ATGTGTGGTT CTCAGATTGG GCTATGCCAT CAGTAATGAG AGTAAACAAG 
AGGACTGGCA AAGATAGAGT ACGTCTCCAA GGCAGCATGC TGAAGCCCTC ATCACTGGTT 
GTGGTTCATC CATTGGCAAA ACCAGGAGCA GATCCCTGCT TATATCAAAA CGGAGGCTGT 
GAACATATTT GCAAAAAGAG GCTTGGAACT GCTTGGTGTT CGTGTCGTGA AGGTTTTATG 
AAAGCCTCAG ATGGGAAAAC GTGTCTGGCT CTGGATGGTC ATCAGCTGTT GGCAGGTGGT 
GAAGTTGATC TAAAGAACCA AGTAACACCA TTGGACATCT TGTCCAAGAC TAGAGTGTCA 
GAAGATAACA TTACAGAATC TCAACACATG CTAGTGGCTG AAATCATGGT GTCAGATCAA 
GATGACTGTG CTCCTGTGGG ATGCAGCATG TATGCTCGGT GTATTTCAGA GGGAGAGGAT 
GCCACATGTC AGTGTTTGAA AGGATTTGCT GGGGATGGAA AACTATGTTC TGATATAGAT 
GAATGTGAGA TGGGTGTCCC AGTGTGCCCC CCTGCCTCCT CCAAGTGCAT CAACACCGAA 
GGTGGTTATG TCTGCCGGTG CTCAGAAGGC TACCAAGGAG ATGGGATTCA CTGTCTTGCT 
GTGTTGTTGG CTACATCGGG GAGCGATGTC AGTACCGAGA CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EGF ( XM_017007854.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016863343.1 Homo sapiens epidermal growth factor (EGF), transcript variant X13, mRNA.

Price & Availability↑

CloneID	OHu94959
Clone ID Related Accession (Same CDS sequence)	XM_017007854.1
Accession Version	XM_017007854.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2136bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	pro-epidermal growth factor isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCTCA CTCTTATCAT TCTGTTGCCA GTAGTTTCAA AATTTAGTTT TGTTAGTCTC 
TCAGCACCGC AGCACTGGAG CTGTCCTGAA GGTACTCTCG CAGGAAATGG GAATTCTACT 
TGTGTGGTTT GGTCTCTTTC TTCCCACCCA GGTCCTGCAC CCTTCTTAAT TTTCTCCCAT 
GGAAATAGTA TCTTTAGGAT TGACACAGAA GGAACCAATT ATGAGCAATT GGTGGTGGAT 
GCTGGTGTCT CAGTGATCAT GGATTTTCAT TATAATGAGA AAAGAATCTA TTGGGTGGAT 
TTAGAAAGAC AACTTTTGCA AAGAGTTTTT CTGAATGGGT CAAGGCAAGA GAGAGTATGT 
AATATAGAGA AAAATGTTTC TGGAATGGCA ATAAATTGGA TAAATGAAGA AGTTATTTGG 
TCAAATCAAC AGGAAGGAAT CATTACAGTA ACAGATATGA AAGGAAATAA TTCCCACATT 
CTTTTAAGTG CTTTAAAATA TCCTGCAAAT GTAGCAGTTG ATCCAGTAGA AAGGTTTATA 
TTTTGGTCTT CAGAGGTGGC TGGAAGCCTT TATAGAGCAG ATCTCGATGG TGTGGGAGTG 
AAGGCTCTGT TGGAGACATC AGAGAAAATA ACAGCTGTGT CATTGGATGT GCTTGATAAG 
CGGCTGTTTT GGATTCAGTA CAACAGAGAA GGAAGCAATT CTCTTATTTG CTCCTGTGAT 
TATGATGGAG GTTCTGTCCA CATTAGTAAA CATCCAACAC AGCATAATTT GTTTGCAATG 
TCCCTTTTTG GTGACCGTAT CTTCTATTCA ACATGGAAAA TGAAGACAAT TTGGATAGCC 
AACAAACACA CTGGAAAGGA CATGGTTAGA ATTAACCTCC ATTCATCATT TGTACCACTT 
GGTGAACTGA AAGTAGTGCA TCCACTTGCA CAACCCAAGG CAGAAGATGA CACTTGGGAG 
CCTGAGCAGA AACTTTGCAA ATTGAGGAAA GGAAACTGCA GCAGCACTGT GTGTGGGCAA 
GACCTCCAGT CACACTTGTG CATGTGTGCA GAGGGATACG CCCTAAGTCG AGACCGGAAG 
TACTGTGAAG ATGTTAATGA ATGTGCTTTT TGGAATCATG GCTGTACTCT TGGGTGTAAA 
AACACCCCTG GATCCTATTA CTGCACGTGC CCTGTAGGAT TTGTTCTGCT TCCTGATGGG 
AAACGATGTC ATCAACTTGT TTCCTGTCCA CGCAATGTGT CTGAATGCAG CCATGACTGT 
GTTCTGACAT CAGAAGGTCC CTTATGTTTC TGTCCTGAAG GCTCAGTGCT TGAGAGAGAT 
GGGAAAACAT GTAGCGGTTG TTCCTCACCC GATAATGGTG GATGTAGCCA GCTCTGCGTT 
CCTCTTAGCC CAGTATCCTG GGAATGTGAT TGCTTTCCTG GGTATGACCT ACAACTGGAT 
GAAAAAAGCT GTGCAGCTTC AGGACCACAA CCATTTTTGC TGTTTGCCAA TTCTCAAGAT 
ATTCGACACA TGCATTTTGA TGGAACAGAC TATGGAACTC TGCTCAGCCA GCAGATGGGA 
ATGGTTTATG CCCTAGATCA TGACCCTGTG GAAAATAAGA TATACTTTGC CCATACAGCC 
CTGAAGTGGA TAGAGAGAGC TAATATGGAT GGTTCCCAGC GAGAAAGGCT TATTGAGGAA 
GGAGTAGATG TGCCAGAAGG TCTTGCTGTG GACTGGATTG GCCGTAGATT CTATTGGACA 
GACAGAGGGA AATCTCTGAT TGGAAGGAGT GATTTAAATG GGAAACGTTC CAAAATAATC 
ACTAAGGAGA ACATCTCTCA ACCACGAGGA ATTGCTGTTC ATCCAATGGC CAAGAGATTA 
TTCTGGACTG ATACAGGGAT TAATCCACGA ATTGAAAGTT CTTCCCTCCA AGGCCTTGGC 
CGTCTGGTTA TAGCCAGCTC TGATCTAATC TGGCCCAGTG GAATAACGAT TGACTTCTTA 
ACTGACAAGT TGTACTGGTG CGATGCCAAG CAGTCTGTGA TTGAAATGGC CAATCTGGAT 
GGTTCAAAAC GCCGAAGACT TACCCAGAAT GATGAAGAAG CTAACCAACT AATAATCTGT 
ATAAGATACC TATTTTGCCC ATTCTCTTTG GCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016863343.1
CDS	454..2589
Translation	MLLTLIILLPVVSKFSFVSLSAPQHWSCPEGTLAGNGNSTCVVWSLSSHPGPAPFLIFSHGNSIFRIDTEGTNYEQLVVDAGVSVIMDFHYNEKRIYWVDLERQLLQRVFLNGSRQERVCNIEKNVSGMAINWINEEVIWSNQQEGIITVTDMKGNNSHILLSALKYPANVAVDPVERFIFWSSEVAGSLYRADLDGVGVKALLETSEKITAVSLDVLDKRLFWIQYNREGSNSLICSCDYDGGSVHISKHPTQHNLFAMSLFGDRIFYSTWKMKTIWIANKHTGKDMVRINLHSSFVPLGELKVVHPLAQPKAEDDTWEPEQKLCKLRKGNCSSTVCGQDLQSHLCMCAEGYALSRDRKYCEDVNECAFWNHGCTLGCKNTPGSYYCTCPVGFVLLPDGKRCHQLVSCPRNVSECSHDCVLTSEGPLCFCPEGSVLERDGKTCSGCSSPDNGGCSQLCVPLSPVSWECDCFPGYDLQLDEKSCAASGPQPFLLFANSQDIRHMHFDGTDYGTLLSQQMGMVYALDHDPVENKIYFAHTALKWIERANMDGSQRERLIEEGVDVPEGLAVDWIGRRFYWTDRGKSLIGRSDLNGKRSKIITKENISQPRGIAVHPMAKRLFWTDTGINPRIESSSLQGLGRLVIASSDLIWPSGITIDFLTDKLYWCDAKQSVIEMANLDGSKRRRLTQNDEEANQLIICIRYLFCPFSLA

Target ORF information:

RefSeq Version	XM_017007854.1
Organism	Homo sapiens(human)
Definition	Homo sapiens epidermal growth factor (EGF), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017007854.1

ORF Insert Sequence:

ATGCTGCTCA CTCTTATCAT TCTGTTGCCA GTAGTTTCAA AATTTAGTTT TGTTAGTCTC 
TCAGCACCGC AGCACTGGAG CTGTCCTGAA GGTACTCTCG CAGGAAATGG GAATTCTACT 
TGTGTGGTTT GGTCTCTTTC TTCCCACCCA GGTCCTGCAC CCTTCTTAAT TTTCTCCCAT 
GGAAATAGTA TCTTTAGGAT TGACACAGAA GGAACCAATT ATGAGCAATT GGTGGTGGAT 
GCTGGTGTCT CAGTGATCAT GGATTTTCAT TATAATGAGA AAAGAATCTA TTGGGTGGAT 
TTAGAAAGAC AACTTTTGCA AAGAGTTTTT CTGAATGGGT CAAGGCAAGA GAGAGTATGT 
AATATAGAGA AAAATGTTTC TGGAATGGCA ATAAATTGGA TAAATGAAGA AGTTATTTGG 
TCAAATCAAC AGGAAGGAAT CATTACAGTA ACAGATATGA AAGGAAATAA TTCCCACATT 
CTTTTAAGTG CTTTAAAATA TCCTGCAAAT GTAGCAGTTG ATCCAGTAGA AAGGTTTATA 
TTTTGGTCTT CAGAGGTGGC TGGAAGCCTT TATAGAGCAG ATCTCGATGG TGTGGGAGTG 
AAGGCTCTGT TGGAGACATC AGAGAAAATA ACAGCTGTGT CATTGGATGT GCTTGATAAG 
CGGCTGTTTT GGATTCAGTA CAACAGAGAA GGAAGCAATT CTCTTATTTG CTCCTGTGAT 
TATGATGGAG GTTCTGTCCA CATTAGTAAA CATCCAACAC AGCATAATTT GTTTGCAATG 
TCCCTTTTTG GTGACCGTAT CTTCTATTCA ACATGGAAAA TGAAGACAAT TTGGATAGCC 
AACAAACACA CTGGAAAGGA CATGGTTAGA ATTAACCTCC ATTCATCATT TGTACCACTT 
GGTGAACTGA AAGTAGTGCA TCCACTTGCA CAACCCAAGG CAGAAGATGA CACTTGGGAG 
CCTGAGCAGA AACTTTGCAA ATTGAGGAAA GGAAACTGCA GCAGCACTGT GTGTGGGCAA 
GACCTCCAGT CACACTTGTG CATGTGTGCA GAGGGATACG CCCTAAGTCG AGACCGGAAG 
TACTGTGAAG ATGTTAATGA ATGTGCTTTT TGGAATCATG GCTGTACTCT TGGGTGTAAA 
AACACCCCTG GATCCTATTA CTGCACGTGC CCTGTAGGAT TTGTTCTGCT TCCTGATGGG 
AAACGATGTC ATCAACTTGT TTCCTGTCCA CGCAATGTGT CTGAATGCAG CCATGACTGT 
GTTCTGACAT CAGAAGGTCC CTTATGTTTC TGTCCTGAAG GCTCAGTGCT TGAGAGAGAT 
GGGAAAACAT GTAGCGGTTG TTCCTCACCC GATAATGGTG GATGTAGCCA GCTCTGCGTT 
CCTCTTAGCC CAGTATCCTG GGAATGTGAT TGCTTTCCTG GGTATGACCT ACAACTGGAT 
GAAAAAAGCT GTGCAGCTTC AGGACCACAA CCATTTTTGC TGTTTGCCAA TTCTCAAGAT 
ATTCGACACA TGCATTTTGA TGGAACAGAC TATGGAACTC TGCTCAGCCA GCAGATGGGA 
ATGGTTTATG CCCTAGATCA TGACCCTGTG GAAAATAAGA TATACTTTGC CCATACAGCC 
CTGAAGTGGA TAGAGAGAGC TAATATGGAT GGTTCCCAGC GAGAAAGGCT TATTGAGGAA 
GGAGTAGATG TGCCAGAAGG TCTTGCTGTG GACTGGATTG GCCGTAGATT CTATTGGACA 
GACAGAGGGA AATCTCTGAT TGGAAGGAGT GATTTAAATG GGAAACGTTC CAAAATAATC 
ACTAAGGAGA ACATCTCTCA ACCACGAGGA ATTGCTGTTC ATCCAATGGC CAAGAGATTA 
TTCTGGACTG ATACAGGGAT TAATCCACGA ATTGAAAGTT CTTCCCTCCA AGGCCTTGGC 
CGTCTGGTTA TAGCCAGCTC TGATCTAATC TGGCCCAGTG GAATAACGAT TGACTTCTTA 
ACTGACAAGT TGTACTGGTG CGATGCCAAG CAGTCTGTGA TTGAAATGGC CAATCTGGAT 
GGTTCAAAAC GCCGAAGACT TACCCAGAAT GATGAAGAAG CTAACCAACT AATAATCTGT 
ATAAGATACC TATTTTGCCC ATTCTCTTTG GCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EGF ( XM_017007855.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016863344.1 Homo sapiens epidermal growth factor (EGF), transcript variant X14, mRNA.

Price & Availability↑

CloneID	OHu94960
Clone ID Related Accession (Same CDS sequence)	XM_017007855.1
Accession Version	XM_017007855.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2091bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	pro-epidermal growth factor isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCTCA CTCTTATCAT TCTGTTGCCA GTAGTTTCAA AATTTAGTTT TGTTAGTCTC 
TCAGCACCGC AGCACTGGAG CTGTCCTGAA GGTACTCTCG CAGGAAATGG GAATTCTACT 
TGTGTGGTTT GGTCTCTTTC TTCCCACCCA GGTCCTGCAC CCTTCTTAAT TTTCTCCCAT 
GGAAATAGTA TCTTTAGGAT TGACACAGAA GGAACCAATT ATGAGCAATT GGTGGTGGAT 
GCTGGTGTCT CAGTGATCAT GGATTTTCAT TATAATGAGA AAAGAATCTA TTGGGTGGAT 
TTAGAAAGAC AACTTTTGCA AAGAGTTTTT CTGAATGGGT CAAGGCAAGA GAGAGTATGT 
AATATAGAGA AAAATGTTTC TGGAATGGCA ATAAATTGGA TAAATGAAGA AGTTATTTGG 
TCAAATCAAC AGGAAGGAAT CATTACAGTA ACAGATATGA AAGGAAATAA TTCCCACATT 
CTTTTAAGTG CTTTAAAATA TCCTGCAAAT GTAGCAGTTG ATCCAGTAGA AAGGTTTATA 
TTTTGGTCTT CAGAGGTGGC TGGAAGCCTT TATAGAGCAG ATCTCGATGG TGTGGGAGTG 
AAGGCTCTGT TGGAGACATC AGAGAAAATA ACAGCTGTGT CATTGGATGT GCTTGATAAG 
CGGCTGTTTT GGATTCAGTA CAACAGAGAA GGAAGCAATT CTCTTATTTG CTCCTGTGAT 
TATGATGGAG GTTCTGTCCA CATTAGTAAA CATCCAACAC AGCATAATTT GTTTGCAATG 
TCCCTTTTTG GTGACCGTAT CTTCTATTCA ACATGGAAAA TGAAGACAAT TTGGATAGCC 
AACAAACACA CTGGAAAGGA CATGGTTAGA ATTAACCTCC ATTCATCATT TGTACCACTT 
GGTGAACTGA AAGTAGTGCA TCCACTTGCA CAACCCAAGG CAGAAGATGA CACTTGGGAG 
CCTGAGCAGA AACTTTGCAA ATTGAGGAAA GGAAACTGCA GCAGCACTGT GTGTGGGCAA 
GACCTCCAGT CACACTTGTG CATGTGTGCA GAGGGATACG CCCTAAGTCG AGACCGGAAG 
TACTGTGAAG ATGTTAATGA ATGTGCTTTT TGGAATCATG GCTGTACTCT TGGGTGTAAA 
AACACCCCTG GATCCTATTA CTGCACGTGC CCTGTAGGAT TTGTTCTGCT TCCTGATGGG 
AAACGATGTC ATCAACTTGT TTCCTGTCCA CGCAATGTGT CTGAATGCAG CCATGACTGT 
GTTCTGACAT CAGAAGGTCC CTTATGTTTC TGTCCTGAAG GCTCAGTGCT TGAGAGAGAT 
GGGAAAACAT GTAGCGGTTG TTCCTCACCC GATAATGGTG GATGTAGCCA GCTCTGCGTT 
CCTCTTAGCC CAGTATCCTG GGAATGTGAT TGCTTTCCTG GGTATGACCT ACAACTGGAT 
GAAAAAAGCT GTGCAGCTTC AGGACCACAA CCATTTTTGC TGTTTGCCAA TTCTCAAGAT 
ATTCGACACA TGCATTTTGA TGGAACAGAC TATGGAACTC TGCTCAGCCA GCAGATGGGA 
ATGGTTTATG CCCTAGATCA TGACCCTGTG GAAAATAAGA TATACTTTGC CCATACAGCC 
CTGAAGTGGA TAGAGAGAGC TAATATGGAT GGTTCCCAGC GAGAAAGGCT TATTGAGGAA 
GGAGTAGATG TGCCAGAAGG TCTTGCTGTG GACTGGATTG GCCGTAGATT CTATTGGACA 
GACAGAGGGA AATCTCTGAT TGGAAGGAGT GATTTAAATG GGAAACGTTC CAAAATAATC 
ACTAAGGAGA ACATCTCTCA ACCACGAGGA ATTGCTGTTC ATCCAATGGC CAAGAGATTA 
TTCTGGACTG ATACAGGGAT TAATCCACGA ATTGAAAGTT CTTCCCTCCA AGGCCTTGGC 
CGTCTGGTTA TAGCCAGCTC TGATCTAATC TGGCCCAGTG GAATAACGAT TGACTTCTTA 
ACTGACAAGT TGTACTGGTG CGATGCCAAG CAGTCTGTGA TTGAAATGGC CAATCTGGAT 
GGTTCAAAAC GCCGAAGACT TACCCAGAAT GATGTCACCC ATTTGCTGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016863344.1
CDS	454..2544
Translation	MLLTLIILLPVVSKFSFVSLSAPQHWSCPEGTLAGNGNSTCVVWSLSSHPGPAPFLIFSHGNSIFRIDTEGTNYEQLVVDAGVSVIMDFHYNEKRIYWVDLERQLLQRVFLNGSRQERVCNIEKNVSGMAINWINEEVIWSNQQEGIITVTDMKGNNSHILLSALKYPANVAVDPVERFIFWSSEVAGSLYRADLDGVGVKALLETSEKITAVSLDVLDKRLFWIQYNREGSNSLICSCDYDGGSVHISKHPTQHNLFAMSLFGDRIFYSTWKMKTIWIANKHTGKDMVRINLHSSFVPLGELKVVHPLAQPKAEDDTWEPEQKLCKLRKGNCSSTVCGQDLQSHLCMCAEGYALSRDRKYCEDVNECAFWNHGCTLGCKNTPGSYYCTCPVGFVLLPDGKRCHQLVSCPRNVSECSHDCVLTSEGPLCFCPEGSVLERDGKTCSGCSSPDNGGCSQLCVPLSPVSWECDCFPGYDLQLDEKSCAASGPQPFLLFANSQDIRHMHFDGTDYGTLLSQQMGMVYALDHDPVENKIYFAHTALKWIERANMDGSQRERLIEEGVDVPEGLAVDWIGRRFYWTDRGKSLIGRSDLNGKRSKIITKENISQPRGIAVHPMAKRLFWTDTGINPRIESSSLQGLGRLVIASSDLIWPSGITIDFLTDKLYWCDAKQSVIEMANLDGSKRRRLTQNDVTHLL

Target ORF information:

RefSeq Version	XM_017007855.1
Organism	Homo sapiens(human)
Definition	Homo sapiens epidermal growth factor (EGF), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017007855.1

ORF Insert Sequence:

ATGCTGCTCA CTCTTATCAT TCTGTTGCCA GTAGTTTCAA AATTTAGTTT TGTTAGTCTC 
TCAGCACCGC AGCACTGGAG CTGTCCTGAA GGTACTCTCG CAGGAAATGG GAATTCTACT 
TGTGTGGTTT GGTCTCTTTC TTCCCACCCA GGTCCTGCAC CCTTCTTAAT TTTCTCCCAT 
GGAAATAGTA TCTTTAGGAT TGACACAGAA GGAACCAATT ATGAGCAATT GGTGGTGGAT 
GCTGGTGTCT CAGTGATCAT GGATTTTCAT TATAATGAGA AAAGAATCTA TTGGGTGGAT 
TTAGAAAGAC AACTTTTGCA AAGAGTTTTT CTGAATGGGT CAAGGCAAGA GAGAGTATGT 
AATATAGAGA AAAATGTTTC TGGAATGGCA ATAAATTGGA TAAATGAAGA AGTTATTTGG 
TCAAATCAAC AGGAAGGAAT CATTACAGTA ACAGATATGA AAGGAAATAA TTCCCACATT 
CTTTTAAGTG CTTTAAAATA TCCTGCAAAT GTAGCAGTTG ATCCAGTAGA AAGGTTTATA 
TTTTGGTCTT CAGAGGTGGC TGGAAGCCTT TATAGAGCAG ATCTCGATGG TGTGGGAGTG 
AAGGCTCTGT TGGAGACATC AGAGAAAATA ACAGCTGTGT CATTGGATGT GCTTGATAAG 
CGGCTGTTTT GGATTCAGTA CAACAGAGAA GGAAGCAATT CTCTTATTTG CTCCTGTGAT 
TATGATGGAG GTTCTGTCCA CATTAGTAAA CATCCAACAC AGCATAATTT GTTTGCAATG 
TCCCTTTTTG GTGACCGTAT CTTCTATTCA ACATGGAAAA TGAAGACAAT TTGGATAGCC 
AACAAACACA CTGGAAAGGA CATGGTTAGA ATTAACCTCC ATTCATCATT TGTACCACTT 
GGTGAACTGA AAGTAGTGCA TCCACTTGCA CAACCCAAGG CAGAAGATGA CACTTGGGAG 
CCTGAGCAGA AACTTTGCAA ATTGAGGAAA GGAAACTGCA GCAGCACTGT GTGTGGGCAA 
GACCTCCAGT CACACTTGTG CATGTGTGCA GAGGGATACG CCCTAAGTCG AGACCGGAAG 
TACTGTGAAG ATGTTAATGA ATGTGCTTTT TGGAATCATG GCTGTACTCT TGGGTGTAAA 
AACACCCCTG GATCCTATTA CTGCACGTGC CCTGTAGGAT TTGTTCTGCT TCCTGATGGG 
AAACGATGTC ATCAACTTGT TTCCTGTCCA CGCAATGTGT CTGAATGCAG CCATGACTGT 
GTTCTGACAT CAGAAGGTCC CTTATGTTTC TGTCCTGAAG GCTCAGTGCT TGAGAGAGAT 
GGGAAAACAT GTAGCGGTTG TTCCTCACCC GATAATGGTG GATGTAGCCA GCTCTGCGTT 
CCTCTTAGCC CAGTATCCTG GGAATGTGAT TGCTTTCCTG GGTATGACCT ACAACTGGAT 
GAAAAAAGCT GTGCAGCTTC AGGACCACAA CCATTTTTGC TGTTTGCCAA TTCTCAAGAT 
ATTCGACACA TGCATTTTGA TGGAACAGAC TATGGAACTC TGCTCAGCCA GCAGATGGGA 
ATGGTTTATG CCCTAGATCA TGACCCTGTG GAAAATAAGA TATACTTTGC CCATACAGCC 
CTGAAGTGGA TAGAGAGAGC TAATATGGAT GGTTCCCAGC GAGAAAGGCT TATTGAGGAA 
GGAGTAGATG TGCCAGAAGG TCTTGCTGTG GACTGGATTG GCCGTAGATT CTATTGGACA 
GACAGAGGGA AATCTCTGAT TGGAAGGAGT GATTTAAATG GGAAACGTTC CAAAATAATC 
ACTAAGGAGA ACATCTCTCA ACCACGAGGA ATTGCTGTTC ATCCAATGGC CAAGAGATTA 
TTCTGGACTG ATACAGGGAT TAATCCACGA ATTGAAAGTT CTTCCCTCCA AGGCCTTGGC 
CGTCTGGTTA TAGCCAGCTC TGATCTAATC TGGCCCAGTG GAATAACGAT TGACTTCTTA 
ACTGACAAGT TGTACTGGTG CGATGCCAAG CAGTCTGTGA TTGAAATGGC CAATCTGGAT 
GGTTCAAAAC GCCGAAGACT TACCCAGAAT GATGTCACCC ATTTGCTGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EGF ( NM_001357021.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001343950.1 Homo sapiens epidermal growth factor (EGF), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OHu94957
Clone ID Related Accession (Same CDS sequence)	XM_017007852.1 , NM_001357021.2 , NM_001357021.1
Accession Version	NM_001357021.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3033bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-31
Organism	Homo sapiens(human)
Product	pro-epidermal growth factor isoform 4 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC005509.1, AK299306.1, AC004050.1 and CA437074.1. On May 31, 2019 this sequence version replaced NM_001357021.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.275536.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1970526, SAMEA2145774 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCTGCTCA CTCTTATCAT TCTGTTGCCA GTAGTTTCAA AATTTAGTTT TGTTAGTCTC 
TCAGCACCGC AGCACTGGAG CTGTCCTGAA GGTACTCTCG CAGGAAATGG GAATTCTACT 
TGTGTGGGTC CTGCACCCTT CTTAATTTTC TCCCATGGAA ATAGTATCTT TAGGATTGAC 
ACAGAAGGAA CCAATTATGA GCAATTGGTG GTGGATGCTG GTGTCTCAGT GATCATGGAT 
TTTCATTATA ATGAGAAAAG AATCTATTGG GTGGATTTAG AAAGACAACT TTTGCAAAGA 
GTTTTTCTGA ATGGGTCAAG GCAAGAGAGA GTATGTAATA TAGAGAAAAA TGTTTCTGGA 
ATGGCAATAA ATTGGATAAA TGAAGAAGTT ATTTGGTCAA ATCAACAGGA AGGAATCATT 
ACAGTAACAG ATATGAAAGG AAATAATTCC CACATTCTTT TAAGTGCTTT AAAATATCCT 
GCAAATGTAG CAGTTGATCC AGTAGAAAGG TTTATATTTT GGTCTTCAGA GGTGGCTGGA 
AGCCTTTATA GAGCAGATCT CGATGGTGTG GGAGTGAAGG CTCTGTTGGA GACATCAGAG 
AAAATAACAG CTGTGTCATT GGATGTGCTT GATAAGCGGC TGTTTTGGAT TCAGTACAAC 
AGAGAAGGAA GCAATTCTCT TATTTGCTCC TGTGATTATG ATGGAGGTTC TGTCCACATT 
AGTAAACATC CAACACAGCA TAATTTGTTT GCAATGTCCC TTTTTGGTGA CCGTATCTTC 
TATTCAACAT GGAAAATGAA GACAATTTGG ATAGCCAACA AACACACTGG AAAGGACATG 
GTTAGAATTA ACCTCCATTC ATCATTTGTA CCACTTGGTG AACTGAAAGT AGTGCATCCA 
CTTGCACAAC CCAAGGCAGA AGATGACACT TGGGAGCCTG ATGTTAATGA ATGTGCTTTT 
TGGAATCATG GCTGTACTCT TGGGTGTAAA AACACCCCTG GATCCTATTA CTGCACGTGC 
CCTGTAGGAT TTGTTCTGCT TCCTGATGGG AAACGATGTC ATCAACTTGT TTCCTGTCCA 
CGCAATGTGT CTGAATGCAG CCATGACTGT GTTCTGACAT CAGAAGGTCC CTTATGTTTC 
TGTCCTGAAG GCTCAGTGCT TGAGAGAGAT GGGAAAACAT GTAGCGGTTG TTCCTCACCC 
GATAATGGTG GATGTAGCCA GCTCTGCGTT CCTCTTAGCC CAGTATCCTG GGAATGTGAT 
TGCTTTCCTG GGTATGACCT ACAACTGGAT GAAAAAAGCT GTGCAGCTTC AGGACCACAA 
CCATTTTTGC TGTTTGCCAA TTCTCAAGAT ATTCGACACA TGCATTTTGA TGGAACAGAC 
TATGGAACTC TGCTCAGCCA GCAGATGGGA ATGGTTTATG CCCTAGATCA TGACCCTGTG 
GAAAATAAGA TATACTTTGC CCATACAGCC CTGAAGTGGA TAGAGAGAGC TAATATGGAT 
GGTTCCCAGC GAGAAAGGCT TATTGAGGAA GGAGTAGATG TGCCAGAAGG TCTTGCTGTG 
GACTGGATTG GCCGTAGATT CTATTGGACA GACAGAGGGA AATCTCTGAT TGGAAGGAGT 
GATTTAAATG GGAAACGTTC CAAAATAATC ACTAAGGAGA ACATCTCTCA ACCACGAGGA 
ATTGCTGTTC ATCCAATGGC CAAGAGATTA TTCTGGACTG ATACAGGGAT TAATCCACGA 
ATTGAAAGTT CTTCCCTCCA AGGCCTTGGC CGTCTGGTTA TAGCCAGCTC TGATCTAATC 
TGGCCCAGTG GAATAACGAT TGACTTCTTA ACTGACAAGT TGTACTGGTG CGATGCCAAG 
CAGTCTGTGA TTGAAATGGC CAATCTGGAT GGTTCAAAAC GCCGAAGACT TACCCAGAAT 
GATGTAGGTC ACCCATTTGC TGTAGCAGTG TTTGAGGATT ATGTGTGGTT CTCAGATTGG 
GCTATGCCAT CAGTAATGAG AGTAAACAAG AGGACTGGCA AAGATAGAGT ACGTCTCCAA 
GGCAGCATGC TGAAGCCCTC ATCACTGGTT GTGGTTCATC CATTGGCAAA ACCAGGAGCA 
GATCCCTGCT TATATCAAAA CGGAGGCTGT GAACATATTT GCAAAAAGAG GCTTGGAACT 
GCTTGGTGTT CGTGTCGTGA AGGTTTTATG AAAGCCTCAG ATGGGAAAAC GTGTCTGGCT 
CTGGATGGTC ATCAGCTGTT GGCAGGTGGT GAAGTTGATC TAAAGAACCA AGTAACACCA 
TTGGACATCT TGTCCAAGAC TAGAGTGTCA GAAGATAACA TTACAGAATC TCAACACATG 
CTAGTGGCTG AAATCATGGT GTCAGATATT GATGAGTGCC AACTGGGGGA GCACAGCTGT 
GGAGAGAATG CCAGCTGCAC AAATACAGAG GGAGGCTATA CCTGCATGTG TGCTGGACGC 
CTGTCTGAAC CAGGACTGAT TTGCCCTGAC TCTACTCCAC CCCCTCACCT CAGGGAAGAT 
GACCACCACT ATTCCGTAAG AAATAGTGAC TCTGAATGTC CCCTGTCCCA CGATGGGTAC 
TGCCTCCATG ATGGTGTGTG CATGTATATT GAAGCATTGG ACAAGTATGC ATGCAACTGT 
GTTGTTGGCT ACATCGGGGA GCGATGTCAG TACCGAGACC TGAAGTGGTG GGAACTGCGC 
CACGCTGGCC ACGGGCAGCA GCAGAAGGTC ATCGTGGTGG CTGTCTGCGT GGTGGTGCTT 
GTCATGCTGC TCCTCCTGAG CCTGTGGGGG GCCCACTACT ACAGGACTCA GAAGCTGCTA 
TCGAAAAACC CAAAGAATCC TTATGAGGAG TCGAGCAGAG ATGTGAGGAG TCGCAGGCCT 
GCTGACACTG AGGATGGGAT GTCCTCTTGC CCTCAACCTT GGGGTCAATG CAACCAACTT 
CATGGAGGCA GGAGCCCCAG TTATGTGGAA TGGGCACAGA GCAAGGCTGC TGGATTCCAG 
TATCCAGTGA TAAGGGCTCC TGTCCCCAGG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001343950.1
CDS	454..3486
Translation	MLLTLIILLPVVSKFSFVSLSAPQHWSCPEGTLAGNGNSTCVGPAPFLIFSHGNSIFRIDTEGTNYEQLVVDAGVSVIMDFHYNEKRIYWVDLERQLLQRVFLNGSRQERVCNIEKNVSGMAINWINEEVIWSNQQEGIITVTDMKGNNSHILLSALKYPANVAVDPVERFIFWSSEVAGSLYRADLDGVGVKALLETSEKITAVSLDVLDKRLFWIQYNREGSNSLICSCDYDGGSVHISKHPTQHNLFAMSLFGDRIFYSTWKMKTIWIANKHTGKDMVRINLHSSFVPLGELKVVHPLAQPKAEDDTWEPDVNECAFWNHGCTLGCKNTPGSYYCTCPVGFVLLPDGKRCHQLVSCPRNVSECSHDCVLTSEGPLCFCPEGSVLERDGKTCSGCSSPDNGGCSQLCVPLSPVSWECDCFPGYDLQLDEKSCAASGPQPFLLFANSQDIRHMHFDGTDYGTLLSQQMGMVYALDHDPVENKIYFAHTALKWIERANMDGSQRERLIEEGVDVPEGLAVDWIGRRFYWTDRGKSLIGRSDLNGKRSKIITKENISQPRGIAVHPMAKRLFWTDTGINPRIESSSLQGLGRLVIASSDLIWPSGITIDFLTDKLYWCDAKQSVIEMANLDGSKRRRLTQNDVGHPFAVAVFEDYVWFSDWAMPSVMRVNKRTGKDRVRLQGSMLKPSSLVVVHPLAKPGADPCLYQNGGCEHICKKRLGTAWCSCREGFMKASDGKTCLALDGHQLLAGGEVDLKNQVTPLDILSKTRVSEDNITESQHMLVAEIMVSDIDECQLGEHSCGENASCTNTEGGYTCMCAGRLSEPGLICPDSTPPPHLREDDHHYSVRNSDSECPLSHDGYCLHDGVCMYIEALDKYACNCVVGYIGERCQYRDLKWWELRHAGHGQQQKVIVVAVCVVVLVMLLLLSLWGAHYYRTQKLLSKNPKNPYEESSRDVRSRRPADTEDGMSSCPQPWGQCNQLHGGRSPSYVEWAQSKAAGFQYPVIRAPVPR

Target ORF information:

RefSeq Version	NM_001357021.2
Organism	Homo sapiens(human)
Definition	Homo sapiens epidermal growth factor (EGF), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001357021.2

ORF Insert Sequence:

ATGCTGCTCA CTCTTATCAT TCTGTTGCCA GTAGTTTCAA AATTTAGTTT TGTTAGTCTC 
TCAGCACCGC AGCACTGGAG CTGTCCTGAA GGTACTCTCG CAGGAAATGG GAATTCTACT 
TGTGTGGGTC CTGCACCCTT CTTAATTTTC TCCCATGGAA ATAGTATCTT TAGGATTGAC 
ACAGAAGGAA CCAATTATGA GCAATTGGTG GTGGATGCTG GTGTCTCAGT GATCATGGAT 
TTTCATTATA ATGAGAAAAG AATCTATTGG GTGGATTTAG AAAGACAACT TTTGCAAAGA 
GTTTTTCTGA ATGGGTCAAG GCAAGAGAGA GTATGTAATA TAGAGAAAAA TGTTTCTGGA 
ATGGCAATAA ATTGGATAAA TGAAGAAGTT ATTTGGTCAA ATCAACAGGA AGGAATCATT 
ACAGTAACAG ATATGAAAGG AAATAATTCC CACATTCTTT TAAGTGCTTT AAAATATCCT 
GCAAATGTAG CAGTTGATCC AGTAGAAAGG TTTATATTTT GGTCTTCAGA GGTGGCTGGA 
AGCCTTTATA GAGCAGATCT CGATGGTGTG GGAGTGAAGG CTCTGTTGGA GACATCAGAG 
AAAATAACAG CTGTGTCATT GGATGTGCTT GATAAGCGGC TGTTTTGGAT TCAGTACAAC 
AGAGAAGGAA GCAATTCTCT TATTTGCTCC TGTGATTATG ATGGAGGTTC TGTCCACATT 
AGTAAACATC CAACACAGCA TAATTTGTTT GCAATGTCCC TTTTTGGTGA CCGTATCTTC 
TATTCAACAT GGAAAATGAA GACAATTTGG ATAGCCAACA AACACACTGG AAAGGACATG 
GTTAGAATTA ACCTCCATTC ATCATTTGTA CCACTTGGTG AACTGAAAGT AGTGCATCCA 
CTTGCACAAC CCAAGGCAGA AGATGACACT TGGGAGCCTG ATGTTAATGA ATGTGCTTTT 
TGGAATCATG GCTGTACTCT TGGGTGTAAA AACACCCCTG GATCCTATTA CTGCACGTGC 
CCTGTAGGAT TTGTTCTGCT TCCTGATGGG AAACGATGTC ATCAACTTGT TTCCTGTCCA 
CGCAATGTGT CTGAATGCAG CCATGACTGT GTTCTGACAT CAGAAGGTCC CTTATGTTTC 
TGTCCTGAAG GCTCAGTGCT TGAGAGAGAT GGGAAAACAT GTAGCGGTTG TTCCTCACCC 
GATAATGGTG GATGTAGCCA GCTCTGCGTT CCTCTTAGCC CAGTATCCTG GGAATGTGAT 
TGCTTTCCTG GGTATGACCT ACAACTGGAT GAAAAAAGCT GTGCAGCTTC AGGACCACAA 
CCATTTTTGC TGTTTGCCAA TTCTCAAGAT ATTCGACACA TGCATTTTGA TGGAACAGAC 
TATGGAACTC TGCTCAGCCA GCAGATGGGA ATGGTTTATG CCCTAGATCA TGACCCTGTG 
GAAAATAAGA TATACTTTGC CCATACAGCC CTGAAGTGGA TAGAGAGAGC TAATATGGAT 
GGTTCCCAGC GAGAAAGGCT TATTGAGGAA GGAGTAGATG TGCCAGAAGG TCTTGCTGTG 
GACTGGATTG GCCGTAGATT CTATTGGACA GACAGAGGGA AATCTCTGAT TGGAAGGAGT 
GATTTAAATG GGAAACGTTC CAAAATAATC ACTAAGGAGA ACATCTCTCA ACCACGAGGA 
ATTGCTGTTC ATCCAATGGC CAAGAGATTA TTCTGGACTG ATACAGGGAT TAATCCACGA 
ATTGAAAGTT CTTCCCTCCA AGGCCTTGGC CGTCTGGTTA TAGCCAGCTC TGATCTAATC 
TGGCCCAGTG GAATAACGAT TGACTTCTTA ACTGACAAGT TGTACTGGTG CGATGCCAAG 
CAGTCTGTGA TTGAAATGGC CAATCTGGAT GGTTCAAAAC GCCGAAGACT TACCCAGAAT 
GATGTAGGTC ACCCATTTGC TGTAGCAGTG TTTGAGGATT ATGTGTGGTT CTCAGATTGG 
GCTATGCCAT CAGTAATGAG AGTAAACAAG AGGACTGGCA AAGATAGAGT ACGTCTCCAA 
GGCAGCATGC TGAAGCCCTC ATCACTGGTT GTGGTTCATC CATTGGCAAA ACCAGGAGCA 
GATCCCTGCT TATATCAAAA CGGAGGCTGT GAACATATTT GCAAAAAGAG GCTTGGAACT 
GCTTGGTGTT CGTGTCGTGA AGGTTTTATG AAAGCCTCAG ATGGGAAAAC GTGTCTGGCT 
CTGGATGGTC ATCAGCTGTT GGCAGGTGGT GAAGTTGATC TAAAGAACCA AGTAACACCA 
TTGGACATCT TGTCCAAGAC TAGAGTGTCA GAAGATAACA TTACAGAATC TCAACACATG 
CTAGTGGCTG AAATCATGGT GTCAGATATT GATGAGTGCC AACTGGGGGA GCACAGCTGT 
GGAGAGAATG CCAGCTGCAC AAATACAGAG GGAGGCTATA CCTGCATGTG TGCTGGACGC 
CTGTCTGAAC CAGGACTGAT TTGCCCTGAC TCTACTCCAC CCCCTCACCT CAGGGAAGAT 
GACCACCACT ATTCCGTAAG AAATAGTGAC TCTGAATGTC CCCTGTCCCA CGATGGGTAC 
TGCCTCCATG ATGGTGTGTG CATGTATATT GAAGCATTGG ACAAGTATGC ATGCAACTGT 
GTTGTTGGCT ACATCGGGGA GCGATGTCAG TACCGAGACC TGAAGTGGTG GGAACTGCGC 
CACGCTGGCC ACGGGCAGCA GCAGAAGGTC ATCGTGGTGG CTGTCTGCGT GGTGGTGCTT 
GTCATGCTGC TCCTCCTGAG CCTGTGGGGG GCCCACTACT ACAGGACTCA GAAGCTGCTA 
TCGAAAAACC CAAAGAATCC TTATGAGGAG TCGAGCAGAG ATGTGAGGAG TCGCAGGCCT 
GCTGACACTG AGGATGGGAT GTCCTCTTGC CCTCAACCTT GGGGTCAATG CAACCAACTT 
CATGGAGGCA GGAGCCCCAG TTATGTGGAA TGGGCACAGA GCAAGGCTGC TGGATTCCAG 
TATCCAGTGA TAAGGGCTCC TGTCCCCAGG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EGF ( NM_001357021.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001343950.1 Homo sapiens epidermal growth factor (EGF), transcript variant 4, mRNA.

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CloneID	OHu94957
Clone ID Related Accession (Same CDS sequence)	XM_017007852.1 , NM_001357021.2 , NM_001357021.1
Accession Version	NM_001357021.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3033bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-26
Organism	Homo sapiens(human)
Product	pro-epidermal growth factor isoform 4 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK299306.1, AC005509.1, AC004050.1 and CA437074.1. On Oct 16, 2017 this sequence version replaced XM_017007852.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.275536.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1970526, SAMEA2145774 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCTGCTCA CTCTTATCAT TCTGTTGCCA GTAGTTTCAA AATTTAGTTT TGTTAGTCTC 
TCAGCACCGC AGCACTGGAG CTGTCCTGAA GGTACTCTCG CAGGAAATGG GAATTCTACT 
TGTGTGGGTC CTGCACCCTT CTTAATTTTC TCCCATGGAA ATAGTATCTT TAGGATTGAC 
ACAGAAGGAA CCAATTATGA GCAATTGGTG GTGGATGCTG GTGTCTCAGT GATCATGGAT 
TTTCATTATA ATGAGAAAAG AATCTATTGG GTGGATTTAG AAAGACAACT TTTGCAAAGA 
GTTTTTCTGA ATGGGTCAAG GCAAGAGAGA GTATGTAATA TAGAGAAAAA TGTTTCTGGA 
ATGGCAATAA ATTGGATAAA TGAAGAAGTT ATTTGGTCAA ATCAACAGGA AGGAATCATT 
ACAGTAACAG ATATGAAAGG AAATAATTCC CACATTCTTT TAAGTGCTTT AAAATATCCT 
GCAAATGTAG CAGTTGATCC AGTAGAAAGG TTTATATTTT GGTCTTCAGA GGTGGCTGGA 
AGCCTTTATA GAGCAGATCT CGATGGTGTG GGAGTGAAGG CTCTGTTGGA GACATCAGAG 
AAAATAACAG CTGTGTCATT GGATGTGCTT GATAAGCGGC TGTTTTGGAT TCAGTACAAC 
AGAGAAGGAA GCAATTCTCT TATTTGCTCC TGTGATTATG ATGGAGGTTC TGTCCACATT 
AGTAAACATC CAACACAGCA TAATTTGTTT GCAATGTCCC TTTTTGGTGA CCGTATCTTC 
TATTCAACAT GGAAAATGAA GACAATTTGG ATAGCCAACA AACACACTGG AAAGGACATG 
GTTAGAATTA ACCTCCATTC ATCATTTGTA CCACTTGGTG AACTGAAAGT AGTGCATCCA 
CTTGCACAAC CCAAGGCAGA AGATGACACT TGGGAGCCTG ATGTTAATGA ATGTGCTTTT 
TGGAATCATG GCTGTACTCT TGGGTGTAAA AACACCCCTG GATCCTATTA CTGCACGTGC 
CCTGTAGGAT TTGTTCTGCT TCCTGATGGG AAACGATGTC ATCAACTTGT TTCCTGTCCA 
CGCAATGTGT CTGAATGCAG CCATGACTGT GTTCTGACAT CAGAAGGTCC CTTATGTTTC 
TGTCCTGAAG GCTCAGTGCT TGAGAGAGAT GGGAAAACAT GTAGCGGTTG TTCCTCACCC 
GATAATGGTG GATGTAGCCA GCTCTGCGTT CCTCTTAGCC CAGTATCCTG GGAATGTGAT 
TGCTTTCCTG GGTATGACCT ACAACTGGAT GAAAAAAGCT GTGCAGCTTC AGGACCACAA 
CCATTTTTGC TGTTTGCCAA TTCTCAAGAT ATTCGACACA TGCATTTTGA TGGAACAGAC 
TATGGAACTC TGCTCAGCCA GCAGATGGGA ATGGTTTATG CCCTAGATCA TGACCCTGTG 
GAAAATAAGA TATACTTTGC CCATACAGCC CTGAAGTGGA TAGAGAGAGC TAATATGGAT 
GGTTCCCAGC GAGAAAGGCT TATTGAGGAA GGAGTAGATG TGCCAGAAGG TCTTGCTGTG 
GACTGGATTG GCCGTAGATT CTATTGGACA GACAGAGGGA AATCTCTGAT TGGAAGGAGT 
GATTTAAATG GGAAACGTTC CAAAATAATC ACTAAGGAGA ACATCTCTCA ACCACGAGGA 
ATTGCTGTTC ATCCAATGGC CAAGAGATTA TTCTGGACTG ATACAGGGAT TAATCCACGA 
ATTGAAAGTT CTTCCCTCCA AGGCCTTGGC CGTCTGGTTA TAGCCAGCTC TGATCTAATC 
TGGCCCAGTG GAATAACGAT TGACTTCTTA ACTGACAAGT TGTACTGGTG CGATGCCAAG 
CAGTCTGTGA TTGAAATGGC CAATCTGGAT GGTTCAAAAC GCCGAAGACT TACCCAGAAT 
GATGTAGGTC ACCCATTTGC TGTAGCAGTG TTTGAGGATT ATGTGTGGTT CTCAGATTGG 
GCTATGCCAT CAGTAATGAG AGTAAACAAG AGGACTGGCA AAGATAGAGT ACGTCTCCAA 
GGCAGCATGC TGAAGCCCTC ATCACTGGTT GTGGTTCATC CATTGGCAAA ACCAGGAGCA 
GATCCCTGCT TATATCAAAA CGGAGGCTGT GAACATATTT GCAAAAAGAG GCTTGGAACT 
GCTTGGTGTT CGTGTCGTGA AGGTTTTATG AAAGCCTCAG ATGGGAAAAC GTGTCTGGCT 
CTGGATGGTC ATCAGCTGTT GGCAGGTGGT GAAGTTGATC TAAAGAACCA AGTAACACCA 
TTGGACATCT TGTCCAAGAC TAGAGTGTCA GAAGATAACA TTACAGAATC TCAACACATG 
CTAGTGGCTG AAATCATGGT GTCAGATATT GATGAGTGCC AACTGGGGGA GCACAGCTGT 
GGAGAGAATG CCAGCTGCAC AAATACAGAG GGAGGCTATA CCTGCATGTG TGCTGGACGC 
CTGTCTGAAC CAGGACTGAT TTGCCCTGAC TCTACTCCAC CCCCTCACCT CAGGGAAGAT 
GACCACCACT ATTCCGTAAG AAATAGTGAC TCTGAATGTC CCCTGTCCCA CGATGGGTAC 
TGCCTCCATG ATGGTGTGTG CATGTATATT GAAGCATTGG ACAAGTATGC ATGCAACTGT 
GTTGTTGGCT ACATCGGGGA GCGATGTCAG TACCGAGACC TGAAGTGGTG GGAACTGCGC 
CACGCTGGCC ACGGGCAGCA GCAGAAGGTC ATCGTGGTGG CTGTCTGCGT GGTGGTGCTT 
GTCATGCTGC TCCTCCTGAG CCTGTGGGGG GCCCACTACT ACAGGACTCA GAAGCTGCTA 
TCGAAAAACC CAAAGAATCC TTATGAGGAG TCGAGCAGAG ATGTGAGGAG TCGCAGGCCT 
GCTGACACTG AGGATGGGAT GTCCTCTTGC CCTCAACCTT GGGGTCAATG CAACCAACTT 
CATGGAGGCA GGAGCCCCAG TTATGTGGAA TGGGCACAGA GCAAGGCTGC TGGATTCCAG 
TATCCAGTGA TAAGGGCTCC TGTCCCCAGG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001343950.1
CDS	453..3485
Translation	MLLTLIILLPVVSKFSFVSLSAPQHWSCPEGTLAGNGNSTCVGPAPFLIFSHGNSIFRIDTEGTNYEQLVVDAGVSVIMDFHYNEKRIYWVDLERQLLQRVFLNGSRQERVCNIEKNVSGMAINWINEEVIWSNQQEGIITVTDMKGNNSHILLSALKYPANVAVDPVERFIFWSSEVAGSLYRADLDGVGVKALLETSEKITAVSLDVLDKRLFWIQYNREGSNSLICSCDYDGGSVHISKHPTQHNLFAMSLFGDRIFYSTWKMKTIWIANKHTGKDMVRINLHSSFVPLGELKVVHPLAQPKAEDDTWEPDVNECAFWNHGCTLGCKNTPGSYYCTCPVGFVLLPDGKRCHQLVSCPRNVSECSHDCVLTSEGPLCFCPEGSVLERDGKTCSGCSSPDNGGCSQLCVPLSPVSWECDCFPGYDLQLDEKSCAASGPQPFLLFANSQDIRHMHFDGTDYGTLLSQQMGMVYALDHDPVENKIYFAHTALKWIERANMDGSQRERLIEEGVDVPEGLAVDWIGRRFYWTDRGKSLIGRSDLNGKRSKIITKENISQPRGIAVHPMAKRLFWTDTGINPRIESSSLQGLGRLVIASSDLIWPSGITIDFLTDKLYWCDAKQSVIEMANLDGSKRRRLTQNDVGHPFAVAVFEDYVWFSDWAMPSVMRVNKRTGKDRVRLQGSMLKPSSLVVVHPLAKPGADPCLYQNGGCEHICKKRLGTAWCSCREGFMKASDGKTCLALDGHQLLAGGEVDLKNQVTPLDILSKTRVSEDNITESQHMLVAEIMVSDIDECQLGEHSCGENASCTNTEGGYTCMCAGRLSEPGLICPDSTPPPHLREDDHHYSVRNSDSECPLSHDGYCLHDGVCMYIEALDKYACNCVVGYIGERCQYRDLKWWELRHAGHGQQQKVIVVAVCVVVLVMLLLLSLWGAHYYRTQKLLSKNPKNPYEESSRDVRSRRPADTEDGMSSCPQPWGQCNQLHGGRSPSYVEWAQSKAAGFQYPVIRAPVPR

Target ORF information:

RefSeq Version	NM_001357021.1
Organism	Homo sapiens(human)
Definition	Homo sapiens epidermal growth factor (EGF), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001357021.1

ORF Insert Sequence:

ATGCTGCTCA CTCTTATCAT TCTGTTGCCA GTAGTTTCAA AATTTAGTTT TGTTAGTCTC 
TCAGCACCGC AGCACTGGAG CTGTCCTGAA GGTACTCTCG CAGGAAATGG GAATTCTACT 
TGTGTGGGTC CTGCACCCTT CTTAATTTTC TCCCATGGAA ATAGTATCTT TAGGATTGAC 
ACAGAAGGAA CCAATTATGA GCAATTGGTG GTGGATGCTG GTGTCTCAGT GATCATGGAT 
TTTCATTATA ATGAGAAAAG AATCTATTGG GTGGATTTAG AAAGACAACT TTTGCAAAGA 
GTTTTTCTGA ATGGGTCAAG GCAAGAGAGA GTATGTAATA TAGAGAAAAA TGTTTCTGGA 
ATGGCAATAA ATTGGATAAA TGAAGAAGTT ATTTGGTCAA ATCAACAGGA AGGAATCATT 
ACAGTAACAG ATATGAAAGG AAATAATTCC CACATTCTTT TAAGTGCTTT AAAATATCCT 
GCAAATGTAG CAGTTGATCC AGTAGAAAGG TTTATATTTT GGTCTTCAGA GGTGGCTGGA 
AGCCTTTATA GAGCAGATCT CGATGGTGTG GGAGTGAAGG CTCTGTTGGA GACATCAGAG 
AAAATAACAG CTGTGTCATT GGATGTGCTT GATAAGCGGC TGTTTTGGAT TCAGTACAAC 
AGAGAAGGAA GCAATTCTCT TATTTGCTCC TGTGATTATG ATGGAGGTTC TGTCCACATT 
AGTAAACATC CAACACAGCA TAATTTGTTT GCAATGTCCC TTTTTGGTGA CCGTATCTTC 
TATTCAACAT GGAAAATGAA GACAATTTGG ATAGCCAACA AACACACTGG AAAGGACATG 
GTTAGAATTA ACCTCCATTC ATCATTTGTA CCACTTGGTG AACTGAAAGT AGTGCATCCA 
CTTGCACAAC CCAAGGCAGA AGATGACACT TGGGAGCCTG ATGTTAATGA ATGTGCTTTT 
TGGAATCATG GCTGTACTCT TGGGTGTAAA AACACCCCTG GATCCTATTA CTGCACGTGC 
CCTGTAGGAT TTGTTCTGCT TCCTGATGGG AAACGATGTC ATCAACTTGT TTCCTGTCCA 
CGCAATGTGT CTGAATGCAG CCATGACTGT GTTCTGACAT CAGAAGGTCC CTTATGTTTC 
TGTCCTGAAG GCTCAGTGCT TGAGAGAGAT GGGAAAACAT GTAGCGGTTG TTCCTCACCC 
GATAATGGTG GATGTAGCCA GCTCTGCGTT CCTCTTAGCC CAGTATCCTG GGAATGTGAT 
TGCTTTCCTG GGTATGACCT ACAACTGGAT GAAAAAAGCT GTGCAGCTTC AGGACCACAA 
CCATTTTTGC TGTTTGCCAA TTCTCAAGAT ATTCGACACA TGCATTTTGA TGGAACAGAC 
TATGGAACTC TGCTCAGCCA GCAGATGGGA ATGGTTTATG CCCTAGATCA TGACCCTGTG 
GAAAATAAGA TATACTTTGC CCATACAGCC CTGAAGTGGA TAGAGAGAGC TAATATGGAT 
GGTTCCCAGC GAGAAAGGCT TATTGAGGAA GGAGTAGATG TGCCAGAAGG TCTTGCTGTG 
GACTGGATTG GCCGTAGATT CTATTGGACA GACAGAGGGA AATCTCTGAT TGGAAGGAGT 
GATTTAAATG GGAAACGTTC CAAAATAATC ACTAAGGAGA ACATCTCTCA ACCACGAGGA 
ATTGCTGTTC ATCCAATGGC CAAGAGATTA TTCTGGACTG ATACAGGGAT TAATCCACGA 
ATTGAAAGTT CTTCCCTCCA AGGCCTTGGC CGTCTGGTTA TAGCCAGCTC TGATCTAATC 
TGGCCCAGTG GAATAACGAT TGACTTCTTA ACTGACAAGT TGTACTGGTG CGATGCCAAG 
CAGTCTGTGA TTGAAATGGC CAATCTGGAT GGTTCAAAAC GCCGAAGACT TACCCAGAAT 
GATGTAGGTC ACCCATTTGC TGTAGCAGTG TTTGAGGATT ATGTGTGGTT CTCAGATTGG 
GCTATGCCAT CAGTAATGAG AGTAAACAAG AGGACTGGCA AAGATAGAGT ACGTCTCCAA 
GGCAGCATGC TGAAGCCCTC ATCACTGGTT GTGGTTCATC CATTGGCAAA ACCAGGAGCA 
GATCCCTGCT TATATCAAAA CGGAGGCTGT GAACATATTT GCAAAAAGAG GCTTGGAACT 
GCTTGGTGTT CGTGTCGTGA AGGTTTTATG AAAGCCTCAG ATGGGAAAAC GTGTCTGGCT 
CTGGATGGTC ATCAGCTGTT GGCAGGTGGT GAAGTTGATC TAAAGAACCA AGTAACACCA 
TTGGACATCT TGTCCAAGAC TAGAGTGTCA GAAGATAACA TTACAGAATC TCAACACATG 
CTAGTGGCTG AAATCATGGT GTCAGATATT GATGAGTGCC AACTGGGGGA GCACAGCTGT 
GGAGAGAATG CCAGCTGCAC AAATACAGAG GGAGGCTATA CCTGCATGTG TGCTGGACGC 
CTGTCTGAAC CAGGACTGAT TTGCCCTGAC TCTACTCCAC CCCCTCACCT CAGGGAAGAT 
GACCACCACT ATTCCGTAAG AAATAGTGAC TCTGAATGTC CCCTGTCCCA CGATGGGTAC 
TGCCTCCATG ATGGTGTGTG CATGTATATT GAAGCATTGG ACAAGTATGC ATGCAACTGT 
GTTGTTGGCT ACATCGGGGA GCGATGTCAG TACCGAGACC TGAAGTGGTG GGAACTGCGC 
CACGCTGGCC ACGGGCAGCA GCAGAAGGTC ATCGTGGTGG CTGTCTGCGT GGTGGTGCTT 
GTCATGCTGC TCCTCCTGAG CCTGTGGGGG GCCCACTACT ACAGGACTCA GAAGCTGCTA 
TCGAAAAACC CAAAGAATCC TTATGAGGAG TCGAGCAGAG ATGTGAGGAG TCGCAGGCCT 
GCTGACACTG AGGATGGGAT GTCCTCTTGC CCTCAACCTT GGGGTCAATG CAACCAACTT 
CATGGAGGCA GGAGCCCCAG TTATGTGGAA TGGGCACAGA GCAAGGCTGC TGGATTCCAG 
TATCCAGTGA TAAGGGCTCC TGTCCCCAGG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EGF ( XM_017007845.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016863334.1 Homo sapiens epidermal growth factor (EGF), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu94950
Clone ID Related Accession (Same CDS sequence)	XM_017007845.1
Accession Version	XM_017007845.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3672bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	pro-epidermal growth factor isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCTCA CTCTTATCAT TCTGTTGCCA GTAGTTTCAA AATTTAGTTT TGTTAGTCTC 
TCAGCACCGC AGCACTGGAG CTGTCCTGAA GGTACTCTCG CAGGAAATGG GAATTCTACT 
TGTGTGGTTT GGTCTCTTTC TTCCCACCCA GGTCCTGCAC CCTTCTTAAT TTTCTCCCAT 
GGAAATAGTA TCTTTAGGAT TGACACAGAA GGAACCAATT ATGAGCAATT GGTGGTGGAT 
GCTGGTGTCT CAGTGATCAT GGATTTTCAT TATAATGAGA AAAGAATCTA TTGGGTGGAT 
TTAGAAAGAC AACTTTTGCA AAGAGTTTTT CTGAATGGGT CAAGGCAAGA GAGAGTATGT 
AATATAGAGA AAAATGTTTC TGGAATGGCA ATAAATTGGA TAAATGAAGA AGTTATTTGG 
TCAAATCAAC AGGAAGGAAT CATTACAGTA ACAGATATGA AAGGAAATAA TTCCCACATT 
CTTTTAAGTG CTTTAAAATA TCCTGCAAAT GTAGCAGTTG ATCCAGTAGA AAGGTTTATA 
TTTTGGTCTT CAGAGGTGGC TGGAAGCCTT TATAGAGCAG ATCTCGATGG TGTGGGAGTG 
AAGGCTCTGT TGGAGACATC AGAGAAAATA ACAGCTGTGT CATTGGATGT GCTTGATAAG 
CGGCTGTTTT GGATTCAGTA CAACAGAGAA GGAAGCAATT CTCTTATTTG CTCCTGTGAT 
TATGATGGAG GTTCTGTCCA CATTAGTAAA CATCCAACAC AGCATAATTT GTTTGCAATG 
TCCCTTTTTG GTGACCGTAT CTTCTATTCA ACATGGAAAA TGAAGACAAT TTGGATAGCC 
AACAAACACA CTGGAAAGGA CATGGTTAGA ATTAACCTCC ATTCATCATT TGTACCACTT 
GGTGAACTGA AAGTAGTGCA TCCACTTGCA CAACCCAAGG CAGAAGATGA CACTTGGGAG 
CCTGAGCAGA AACTTTGCAA ATTGAGGAAA GGAAACTGCA GCAGCACTGT GTGTGGGCAA 
GACCTCCAGT CACACTTGTG CATGTGTGCA GAGGGATACG CCCTAAGTCG AGACCGGAAG 
TACTGTGAAG ATGTTAATGA ATGTGCTTTT TGGAATCATG GCTGTACTCT TGGGTGTAAA 
AACACCCCTG GATCCTATTA CTGCACGTGC CCTGTAGGAT TTGTTCTGCT TCCTGATGGG 
AAACGATGTC ATCAACTTGT TTCCTGTCCA CGCAATGTGT CTGAATGCAG CCATGACTGT 
GTTCTGACAT CAGAAGGTCC CTTATGTTTC TGTCCTGAAG GCTCAGTGCT TGAGAGAGAT 
GGGAAAACAT GTAGCGGTTG TTCCTCACCC GATAATGGTG GATGTAGCCA GCTCTGCGTT 
CCTCTTAGCC CAGTATCCTG GGAATGTGAT TGCTTTCCTG GGTATGACCT ACAACTGGAT 
GAAAAAAGCT GTGCAGCTTC AGGACCACAA CCATTTTTGC TGTTTGCCAA TTCTCAAGAT 
ATTCGACACA TGCATTTTGA TGGAACAGAC TATGGAACTC TGCTCAGCCA GCAGATGGGA 
ATGGTTTATG CCCTAGATCA TGACCCTGTG GAAAATAAGA TATACTTTGC CCATACAGCC 
CTGAAGTGGA TAGAGAGAGC TAATATGGAT GGTTCCCAGC GAGAAAGGCT TATTGAGGAA 
GGAGTAGATG TGCCAGAAGG TCTTGCTGTG GACTGGATTG GCCGTAGATT CTATTGGACA 
GACAGAGGGA AATCTCTGAT TGGAAGGAGT GATTTAAATG GGAAACGTTC CAAAATAATC 
ACTAAGGAGA ACATCTCTCA ACCACGAGGA ATTGCTGTTC ATCCAATGGC CAAGAGATTA 
TTCTGGACTG ATACAGGGAT TAATCCACGA ATTGAAAGTT CTTCCCTCCA AGGCCTTGGC 
CGTCTGGTTA TAGCCAGCTC TGATCTAATC TGGCCCAGTG GAATAACGAT TGACTTCTTA 
ACTGACAAGT TGTACTGGTG CGATGCCAAG CAGTCTGTGA TTGAAATGGC CAATCTGGAT 
GGTTCAAAAC GCCGAAGACT TACCCAGAAT GATGTAGGTC ACCCATTTGC TGTAGCAGTG 
TTTGAGGATT ATGTGTGGTT CTCAGATTGG GCTATGCCAT CAGTAATGAG AGTAAACAAG 
AGGACTGGCA AAGATAGAGT ACGTCTCCAA GGCAGCATGC TGAAGCCCTC ATCACTGGTT 
GTGGTTCATC CATTGGCAAA ACCAGGAGCA GATCCCTGCT TATATCAAAA CGGAGGCTGT 
GAACATATTT GCAAAAAGAG GCTTGGAACT GCTTGGTGTT CGTGTCGTGA AGGTTTTATG 
AAAGCCTCAG ATGGGAAAAC GTGTCTGGCT CTGGATGGTC ATCAGCTGTT GGCAGGTGGT 
GAAGTTGATC TAAAGAACCA AGTAACACCA TTGGACATCT TGTCCAAGAC TAGAGTGTCA 
GAAGATAACA TTACAGAATC TCAACACATG CTAGTGGCTG AAATCATGGT GTCAGATCAA 
GATGACTGTG CTCCTGTGGG ATGCAGCATG TATGCTCGGT GTATTTCAGA GGGAGAGGAT 
GCCACATGTC AGTGTTTGAA AGGATTTGCT GGGGATGGAA AACTATGTTC TGATATAGAT 
GAATGTGAGA TGGGTGTCCC AGTGTGCCCC CCTGCCTCCT CCAAGTGCAT CAACACCGAA 
GGTGGTTATG TCTGCCGGTG CTCAGAAGGC TACCAAGGAG ATGGGATTCA CTGTCTTGAT 
ATTGATGAGT GCCAACTGGG GGAGCACAGC TGTGGAGAGA ATGCCAGCTG CACAAATACA 
GAGGGAGGCT ATACCTGCAT GTGTGCTGGA CGCCTGTCTG AACCAGGACT GATTTGCCCT 
GACTCTACTC CACCCCCTCA CCTCAGGGAA GATGACCACC ACTATTCCGT AAGAAATAGT 
GACTCTGAAT GTCCCCTGTC CCACGATGGG TACTGCCTCC ATGATGGTGT GTGCATGTAT 
ATTGAAGCAT TGGACAAGTA TGCATGCAAC TGTGTTGTTG GCTACATCGG GGAGCGATGT 
CAGTACCGAG ACCTGAAGTG GTGGGAACTG CGCCACGCTG GCCACGGGCA GCAGCAGAAG 
GTCATCGTGG TGGCTGTCTG CGTGGTGGTG CTTGTCATGC TGCTCCTCCT GAGCCTGTGG 
GGGGCCCACT ACTACAGGAC TCAGAAGCTG CTATCGAAAA ACCCAAAGAA TCCTTATGAG 
GAGTCGAGCA GAGATGTGAG GAGTCGCAGG CCTGCTGACA CTGAGGATGG GATGTCCTCT 
TGCCCTCAAC CTTGGTTTGT GGTTATAAAA GAACACCAAG ACCTCAAGAA TGGGGGTCAA 
CCAGTGGCTG GTGAGGATGG CCAGGCAGCA GATGGTCAGT TTTTATCCCT GGCTCCTGGG 
TCAATGCAAC CAACTTCATG GAGGCAGGAG CCCCAGTTAT GTGGAATGGG CACAGAGCAA 
GGCTGCTGGA TTCCAGTATC CAGTGATAAG GGCTCCTGTC CCCAGGTAAT GGAGCGAAGC 
TTTCATATGC CCTCCTATGG GACACAGACC CTTGAAGGGG GTGTCGAGAA GCCCCATTCT 
CTCCTATCAG CTAACCCATT ATGGCAACAA AGGGCCCTGG ACCCACCACA CCAAATGGAG 
CTGACTCAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016863334.1
CDS	454..4125
Translation	MLLTLIILLPVVSKFSFVSLSAPQHWSCPEGTLAGNGNSTCVVWSLSSHPGPAPFLIFSHGNSIFRIDTEGTNYEQLVVDAGVSVIMDFHYNEKRIYWVDLERQLLQRVFLNGSRQERVCNIEKNVSGMAINWINEEVIWSNQQEGIITVTDMKGNNSHILLSALKYPANVAVDPVERFIFWSSEVAGSLYRADLDGVGVKALLETSEKITAVSLDVLDKRLFWIQYNREGSNSLICSCDYDGGSVHISKHPTQHNLFAMSLFGDRIFYSTWKMKTIWIANKHTGKDMVRINLHSSFVPLGELKVVHPLAQPKAEDDTWEPEQKLCKLRKGNCSSTVCGQDLQSHLCMCAEGYALSRDRKYCEDVNECAFWNHGCTLGCKNTPGSYYCTCPVGFVLLPDGKRCHQLVSCPRNVSECSHDCVLTSEGPLCFCPEGSVLERDGKTCSGCSSPDNGGCSQLCVPLSPVSWECDCFPGYDLQLDEKSCAASGPQPFLLFANSQDIRHMHFDGTDYGTLLSQQMGMVYALDHDPVENKIYFAHTALKWIERANMDGSQRERLIEEGVDVPEGLAVDWIGRRFYWTDRGKSLIGRSDLNGKRSKIITKENISQPRGIAVHPMAKRLFWTDTGINPRIESSSLQGLGRLVIASSDLIWPSGITIDFLTDKLYWCDAKQSVIEMANLDGSKRRRLTQNDVGHPFAVAVFEDYVWFSDWAMPSVMRVNKRTGKDRVRLQGSMLKPSSLVVVHPLAKPGADPCLYQNGGCEHICKKRLGTAWCSCREGFMKASDGKTCLALDGHQLLAGGEVDLKNQVTPLDILSKTRVSEDNITESQHMLVAEIMVSDQDDCAPVGCSMYARCISEGEDATCQCLKGFAGDGKLCSDIDECEMGVPVCPPASSKCINTEGGYVCRCSEGYQGDGIHCLDIDECQLGEHSCGENASCTNTEGGYTCMCAGRLSEPGLICPDSTPPPHLREDDHHYSVRNSDSECPLSHDGYCLHDGVCMYIEALDKYACNCVVGYIGERCQYRDLKWWELRHAGHGQQQKVIVVAVCVVVLVMLLLLSLWGAHYYRTQKLLSKNPKNPYEESSRDVRSRRPADTEDGMSSCPQPWFVVIKEHQDLKNGGQPVAGEDGQAADGQFLSLAPGSMQPTSWRQEPQLCGMGTEQGCWIPVSSDKGSCPQVMERSFHMPSYGTQTLEGGVEKPHSLLSANPLWQQRALDPPHQMELTQ

Target ORF information:

RefSeq Version	XM_017007845.1
Organism	Homo sapiens(human)
Definition	Homo sapiens epidermal growth factor (EGF), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017007845.1

ORF Insert Sequence:

ATGCTGCTCA CTCTTATCAT TCTGTTGCCA GTAGTTTCAA AATTTAGTTT TGTTAGTCTC 
TCAGCACCGC AGCACTGGAG CTGTCCTGAA GGTACTCTCG CAGGAAATGG GAATTCTACT 
TGTGTGGTTT GGTCTCTTTC TTCCCACCCA GGTCCTGCAC CCTTCTTAAT TTTCTCCCAT 
GGAAATAGTA TCTTTAGGAT TGACACAGAA GGAACCAATT ATGAGCAATT GGTGGTGGAT 
GCTGGTGTCT CAGTGATCAT GGATTTTCAT TATAATGAGA AAAGAATCTA TTGGGTGGAT 
TTAGAAAGAC AACTTTTGCA AAGAGTTTTT CTGAATGGGT CAAGGCAAGA GAGAGTATGT 
AATATAGAGA AAAATGTTTC TGGAATGGCA ATAAATTGGA TAAATGAAGA AGTTATTTGG 
TCAAATCAAC AGGAAGGAAT CATTACAGTA ACAGATATGA AAGGAAATAA TTCCCACATT 
CTTTTAAGTG CTTTAAAATA TCCTGCAAAT GTAGCAGTTG ATCCAGTAGA AAGGTTTATA 
TTTTGGTCTT CAGAGGTGGC TGGAAGCCTT TATAGAGCAG ATCTCGATGG TGTGGGAGTG 
AAGGCTCTGT TGGAGACATC AGAGAAAATA ACAGCTGTGT CATTGGATGT GCTTGATAAG 
CGGCTGTTTT GGATTCAGTA CAACAGAGAA GGAAGCAATT CTCTTATTTG CTCCTGTGAT 
TATGATGGAG GTTCTGTCCA CATTAGTAAA CATCCAACAC AGCATAATTT GTTTGCAATG 
TCCCTTTTTG GTGACCGTAT CTTCTATTCA ACATGGAAAA TGAAGACAAT TTGGATAGCC 
AACAAACACA CTGGAAAGGA CATGGTTAGA ATTAACCTCC ATTCATCATT TGTACCACTT 
GGTGAACTGA AAGTAGTGCA TCCACTTGCA CAACCCAAGG CAGAAGATGA CACTTGGGAG 
CCTGAGCAGA AACTTTGCAA ATTGAGGAAA GGAAACTGCA GCAGCACTGT GTGTGGGCAA 
GACCTCCAGT CACACTTGTG CATGTGTGCA GAGGGATACG CCCTAAGTCG AGACCGGAAG 
TACTGTGAAG ATGTTAATGA ATGTGCTTTT TGGAATCATG GCTGTACTCT TGGGTGTAAA 
AACACCCCTG GATCCTATTA CTGCACGTGC CCTGTAGGAT TTGTTCTGCT TCCTGATGGG 
AAACGATGTC ATCAACTTGT TTCCTGTCCA CGCAATGTGT CTGAATGCAG CCATGACTGT 
GTTCTGACAT CAGAAGGTCC CTTATGTTTC TGTCCTGAAG GCTCAGTGCT TGAGAGAGAT 
GGGAAAACAT GTAGCGGTTG TTCCTCACCC GATAATGGTG GATGTAGCCA GCTCTGCGTT 
CCTCTTAGCC CAGTATCCTG GGAATGTGAT TGCTTTCCTG GGTATGACCT ACAACTGGAT 
GAAAAAAGCT GTGCAGCTTC AGGACCACAA CCATTTTTGC TGTTTGCCAA TTCTCAAGAT 
ATTCGACACA TGCATTTTGA TGGAACAGAC TATGGAACTC TGCTCAGCCA GCAGATGGGA 
ATGGTTTATG CCCTAGATCA TGACCCTGTG GAAAATAAGA TATACTTTGC CCATACAGCC 
CTGAAGTGGA TAGAGAGAGC TAATATGGAT GGTTCCCAGC GAGAAAGGCT TATTGAGGAA 
GGAGTAGATG TGCCAGAAGG TCTTGCTGTG GACTGGATTG GCCGTAGATT CTATTGGACA 
GACAGAGGGA AATCTCTGAT TGGAAGGAGT GATTTAAATG GGAAACGTTC CAAAATAATC 
ACTAAGGAGA ACATCTCTCA ACCACGAGGA ATTGCTGTTC ATCCAATGGC CAAGAGATTA 
TTCTGGACTG ATACAGGGAT TAATCCACGA ATTGAAAGTT CTTCCCTCCA AGGCCTTGGC 
CGTCTGGTTA TAGCCAGCTC TGATCTAATC TGGCCCAGTG GAATAACGAT TGACTTCTTA 
ACTGACAAGT TGTACTGGTG CGATGCCAAG CAGTCTGTGA TTGAAATGGC CAATCTGGAT 
GGTTCAAAAC GCCGAAGACT TACCCAGAAT GATGTAGGTC ACCCATTTGC TGTAGCAGTG 
TTTGAGGATT ATGTGTGGTT CTCAGATTGG GCTATGCCAT CAGTAATGAG AGTAAACAAG 
AGGACTGGCA AAGATAGAGT ACGTCTCCAA GGCAGCATGC TGAAGCCCTC ATCACTGGTT 
GTGGTTCATC CATTGGCAAA ACCAGGAGCA GATCCCTGCT TATATCAAAA CGGAGGCTGT 
GAACATATTT GCAAAAAGAG GCTTGGAACT GCTTGGTGTT CGTGTCGTGA AGGTTTTATG 
AAAGCCTCAG ATGGGAAAAC GTGTCTGGCT CTGGATGGTC ATCAGCTGTT GGCAGGTGGT 
GAAGTTGATC TAAAGAACCA AGTAACACCA TTGGACATCT TGTCCAAGAC TAGAGTGTCA 
GAAGATAACA TTACAGAATC TCAACACATG CTAGTGGCTG AAATCATGGT GTCAGATCAA 
GATGACTGTG CTCCTGTGGG ATGCAGCATG TATGCTCGGT GTATTTCAGA GGGAGAGGAT 
GCCACATGTC AGTGTTTGAA AGGATTTGCT GGGGATGGAA AACTATGTTC TGATATAGAT 
GAATGTGAGA TGGGTGTCCC AGTGTGCCCC CCTGCCTCCT CCAAGTGCAT CAACACCGAA 
GGTGGTTATG TCTGCCGGTG CTCAGAAGGC TACCAAGGAG ATGGGATTCA CTGTCTTGAT 
ATTGATGAGT GCCAACTGGG GGAGCACAGC TGTGGAGAGA ATGCCAGCTG CACAAATACA 
GAGGGAGGCT ATACCTGCAT GTGTGCTGGA CGCCTGTCTG AACCAGGACT GATTTGCCCT 
GACTCTACTC CACCCCCTCA CCTCAGGGAA GATGACCACC ACTATTCCGT AAGAAATAGT 
GACTCTGAAT GTCCCCTGTC CCACGATGGG TACTGCCTCC ATGATGGTGT GTGCATGTAT 
ATTGAAGCAT TGGACAAGTA TGCATGCAAC TGTGTTGTTG GCTACATCGG GGAGCGATGT 
CAGTACCGAG ACCTGAAGTG GTGGGAACTG CGCCACGCTG GCCACGGGCA GCAGCAGAAG 
GTCATCGTGG TGGCTGTCTG CGTGGTGGTG CTTGTCATGC TGCTCCTCCT GAGCCTGTGG 
GGGGCCCACT ACTACAGGAC TCAGAAGCTG CTATCGAAAA ACCCAAAGAA TCCTTATGAG 
GAGTCGAGCA GAGATGTGAG GAGTCGCAGG CCTGCTGACA CTGAGGATGG GATGTCCTCT 
TGCCCTCAAC CTTGGTTTGT GGTTATAAAA GAACACCAAG ACCTCAAGAA TGGGGGTCAA 
CCAGTGGCTG GTGAGGATGG CCAGGCAGCA GATGGTCAGT TTTTATCCCT GGCTCCTGGG 
TCAATGCAAC CAACTTCATG GAGGCAGGAG CCCCAGTTAT GTGGAATGGG CACAGAGCAA 
GGCTGCTGGA TTCCAGTATC CAGTGATAAG GGCTCCTGTC CCCAGGTAAT GGAGCGAAGC 
TTTCATATGC CCTCCTATGG GACACAGACC CTTGAAGGGG GTGTCGAGAA GCCCCATTCT 
CTCCTATCAG CTAACCCATT ATGGCAACAA AGGGCCCTGG ACCCACCACA CCAAATGGAG 
CTGACTCAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EGF ( XM_017007846.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016863335.1 Homo sapiens epidermal growth factor (EGF), transcript variant X3, mRNA.

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CloneID	OHu94951
Clone ID Related Accession (Same CDS sequence)	XM_017007846.1
Accession Version	XM_017007846.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3648bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	pro-epidermal growth factor isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCTCA CTCTTATCAT TCTGTTGCCA GTAGTTTCAA AATTTAGTTT TGTTAGTCTC 
TCAGCACCGC AGCACTGGAG CTGTCCTGAA GGTACTCTCG CAGGAAATGG GAATTCTACT 
TGTGTGGTTT GGTCTCTTTC TTCCCACCCA GGTCCTGCAC CCTTCTTAAT TTTCTCCCAT 
GGAAATAGTA TCTTTAGGAT TGACACAGAA GGAACCAATT ATGAGCAATT GGTGGTGGAT 
GCTGGTGTCT CAGTGATCAT GGATTTTCAT TATAATGAGA AAAGAATCTA TTGGGTGGAT 
TTAGAAAGAC AACTTTTGCA AAGAGTTTTT CTGAATGGGT CAAGGCAAGA GAGAGTATGT 
AATATAGAGA AAAATGTTTC TGGAATGGCA ATAAATTGGA TAAATGAAGA AGTTATTTGG 
TCAAATCAAC AGGAAGGAAT CATTACAGTA ACAGATATGA AAGGAAATAA TTCCCACATT 
CTTTTAAGTG CTTTAAAATA TCCTGCAAAT GTAGCAGTTG ATCCAGTAGA AAGGTTTATA 
TTTTGGTCTT CAGAGGTGGC TGGAAGCCTT TATAGAGCAG ATCTCGATGG TGTGGGAGTG 
AAGGCTCTGT TGGAGACATC AGAGAAAATA ACAGCTGTGT CATTGGATGT GCTTGATAAG 
CGGCTGTTTT GGATTCAGTA CAACAGAGAA GGAAGCAATT CTCTTATTTG CTCCTGTGAT 
TATGATGGAG GTTCTGTCCA CATTAGTAAA CATCCAACAC AGCATAATTT GTTTGCAATG 
TCCCTTTTTG GTGACCGTAT CTTCTATTCA ACATGGAAAA TGAAGACAAT TTGGATAGCC 
AACAAACACA CTGGAAAGGA CATGGTTAGA ATTAACCTCC ATTCATCATT TGTACCACTT 
GGTGAACTGA AAGTAGTGCA TCCACTTGCA CAACCCAAGG CAGAAGATGA CACTTGGGAG 
CCTGAGCAGA AACTTTGCAA ATTGAGGAAA GGAAACTGCA GCAGCACTGT GTGTGGGCAA 
GACCTCCAGT CACACTTGTG CATGTGTGCA GAGGGATACG CCCTAAGTCG AGACCGGAAG 
TACTGTGAAG ATGTTAATGA ATGTGCTTTT TGGAATCATG GCTGTACTCT TGGGTGTAAA 
AACACCCCTG GATCCTATTA CTGCACGTGC CCTGTAGGAT TTGTTCTGCT TCCTGATGGG 
AAACGATGTC ATCAACTTGT TTCCTGTCCA CGCAATGTGT CTGAATGCAG CCATGACTGT 
GTTCTGACAT CAGAAGGTCC CTTATGTTTC TGTCCTGAAG GCTCAGTGCT TGAGAGAGAT 
GGGAAAACAT GTAGCGGTTG TTCCTCACCC GATAATGGTG GATGTAGCCA GCTCTGCGTT 
CCTCTTAGCC CAGTATCCTG GGAATGTGAT TGCTTTCCTG GGTATGACCT ACAACTGGAT 
GAAAAAAGCT GTGCAGCTTC AGGACCACAA CCATTTTTGC TGTTTGCCAA TTCTCAAGAT 
ATTCGACACA TGCATTTTGA TGGAACAGAC TATGGAACTC TGCTCAGCCA GCAGATGGGA 
ATGGTTTATG CCCTAGATCA TGACCCTGTG GAAAATAAGA TATACTTTGC CCATACAGCC 
CTGAAGTGGA TAGAGAGAGC TAATATGGAT GGTTCCCAGC GAGAAAGGCT TATTGAGGAA 
GGAGTAGATG TGCCAGAAGG TCTTGCTGTG GACTGGATTG GCCGTAGATT CTATTGGACA 
GACAGAGGGA AATCTCTGAT TGGAAGGAGT GATTTAAATG GGAAACGTTC CAAAATAATC 
ACTAAGGAGA ACATCTCTCA ACCACGAGGA ATTGCTGTTC ATCCAATGGC CAAGAGATTA 
TTCTGGACTG ATACAGGGAT TAATCCACGA ATTGAAAGTT CTTCCCTCCA AGGCCTTGGC 
CGTCTGGTTA TAGCCAGCTC TGATCTAATC TGGCCCAGTG GAATAACGAT TGACTTCTTA 
ACTGACAAGT TGTACTGGTG CGATGCCAAG CAGTCTGTGA TTGAAATGGC CAATCTGGAT 
GGTTCAAAAC GCCGAAGACT TACCCAGAAT GATGTAGGTC ACCCATTTGC TGTAGCAGTG 
TTTGAGGATT ATGTGTGGTT CTCAGATTGG GCTATGCCAT CAGTAATGAG AGTAAACAAG 
AGGACTGGCA AAGATAGAGT ACGTCTCCAA GGCAGCATGC TGAAGCCCTC ATCACTGGTT 
GTGGTTCATC CATTGGCAAA ACCAGGAGCA GATCCCTGCT TATATCAAAA CGGAGGCTGT 
GAACATATTT GCAAAAAGAG GCTTGGAACT GCTTGGTGTT CGTGTCGTGA AGGTTTTATG 
AAAGCCTCAG ATGGGAAAAC GTGTCTGGCT CTGGATGGTC ATCAGCTGTT GGCAGGTGGT 
GAAGTTGATC TAAAGAACCA AGTAACACCA TTGGACATCT TGTCCAAGAC TAGAGTGTCA 
GAAGATAACA TTACAGAATC TCAACACATG CTAGTGGCTG AAATCATGGT GTCAGATCAA 
GATGACTGTG CTCCTGTGGG ATGCAGCATG TATGCTCGGT GTATTTCAGA GGGAGAGGAT 
GCCACATGTC AGTGTTTGAA AGGATTTGCT GGGGATGGAA AACTATGTTC TGATATAGAT 
GAATGTGAGA TGGGTGTCCC AGTGTGCCCC CCTGCCTCCT CCAAGTGCAT CAACACCGAA 
GGTGGTTATG TCTGCCGGTG CTCAGAAGGC TACCAAGGAG ATGGGATTCA CTGTCTTGAT 
ATTGATGAGT GCCAACTGGG GGAGCACAGC TGTGGAGAGA ATGCCAGCTG CACAAATACA 
GAGGGAGGCT ATACCTGCAT GTGTGCTGGA CGCCTGTCTG AACCAGGACT GATTTGCCCT 
GACTCTACTC CACCCCCTCA CCTCAGGGAA GATGACCACC ACTATTCCGT AAGAAATAGT 
GACTCTGAAT GTCCCCTGTC CCACGATGGG TACTGCCTCC ATGATGGTGT GTGCATGTAT 
ATTGAAGCAT TGGACAAGTA TGCATGCAAC TGTGTTGTTG GCTACATCGG GGAGCGATGT 
CAGTACCGAG ACCTGAAGTG GTGGGAACTG CGCCACGCTG GCCACGGGCA GCAGCAGAAG 
GTCATCGTGG TGGCTGTCTG CGTGGTGGTG CTTGTCATGC TGCTCCTCCT GAGCCTGTGG 
GGGGCCCACT ACTACAGGAC TCAGAAGCTG CTATCGAAAA ACCCAAAGAA TCCTTATGAG 
GAGTCGAGCA GAGATGTGAG GAGTCGCAGG CCTGCTGACA CTGAGGATGG GATGTCCTCT 
TGCCCTCAAC CTTGGTTTGT GGTTATAAAA GAACACCAAG ACCTCAAGAA TGGGGGTCAA 
CCAGTGGCTG GTGAGGATGG CCAGGCAGCA GATGGGTCAA TGCAACCAAC TTCATGGAGG 
CAGGAGCCCC AGTTATGTGG AATGGGCACA GAGCAAGGCT GCTGGATTCC AGTATCCAGT 
GATAAGGGCT CCTGTCCCCA GGTAATGGAG CGAAGCTTTC ATATGCCCTC CTATGGGACA 
CAGACCCTTG AAGGGGGTGT CGAGAAGCCC CATTCTCTCC TATCAGCTAA CCCATTATGG 
CAACAAAGGG CCCTGGACCC ACCACACCAA ATGGAGCTGA CTCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016863335.1
CDS	454..4101
Translation	MLLTLIILLPVVSKFSFVSLSAPQHWSCPEGTLAGNGNSTCVVWSLSSHPGPAPFLIFSHGNSIFRIDTEGTNYEQLVVDAGVSVIMDFHYNEKRIYWVDLERQLLQRVFLNGSRQERVCNIEKNVSGMAINWINEEVIWSNQQEGIITVTDMKGNNSHILLSALKYPANVAVDPVERFIFWSSEVAGSLYRADLDGVGVKALLETSEKITAVSLDVLDKRLFWIQYNREGSNSLICSCDYDGGSVHISKHPTQHNLFAMSLFGDRIFYSTWKMKTIWIANKHTGKDMVRINLHSSFVPLGELKVVHPLAQPKAEDDTWEPEQKLCKLRKGNCSSTVCGQDLQSHLCMCAEGYALSRDRKYCEDVNECAFWNHGCTLGCKNTPGSYYCTCPVGFVLLPDGKRCHQLVSCPRNVSECSHDCVLTSEGPLCFCPEGSVLERDGKTCSGCSSPDNGGCSQLCVPLSPVSWECDCFPGYDLQLDEKSCAASGPQPFLLFANSQDIRHMHFDGTDYGTLLSQQMGMVYALDHDPVENKIYFAHTALKWIERANMDGSQRERLIEEGVDVPEGLAVDWIGRRFYWTDRGKSLIGRSDLNGKRSKIITKENISQPRGIAVHPMAKRLFWTDTGINPRIESSSLQGLGRLVIASSDLIWPSGITIDFLTDKLYWCDAKQSVIEMANLDGSKRRRLTQNDVGHPFAVAVFEDYVWFSDWAMPSVMRVNKRTGKDRVRLQGSMLKPSSLVVVHPLAKPGADPCLYQNGGCEHICKKRLGTAWCSCREGFMKASDGKTCLALDGHQLLAGGEVDLKNQVTPLDILSKTRVSEDNITESQHMLVAEIMVSDQDDCAPVGCSMYARCISEGEDATCQCLKGFAGDGKLCSDIDECEMGVPVCPPASSKCINTEGGYVCRCSEGYQGDGIHCLDIDECQLGEHSCGENASCTNTEGGYTCMCAGRLSEPGLICPDSTPPPHLREDDHHYSVRNSDSECPLSHDGYCLHDGVCMYIEALDKYACNCVVGYIGERCQYRDLKWWELRHAGHGQQQKVIVVAVCVVVLVMLLLLSLWGAHYYRTQKLLSKNPKNPYEESSRDVRSRRPADTEDGMSSCPQPWFVVIKEHQDLKNGGQPVAGEDGQAADGSMQPTSWRQEPQLCGMGTEQGCWIPVSSDKGSCPQVMERSFHMPSYGTQTLEGGVEKPHSLLSANPLWQQRALDPPHQMELTQ

Target ORF information:

RefSeq Version	XM_017007846.1
Organism	Homo sapiens(human)
Definition	Homo sapiens epidermal growth factor (EGF), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017007846.1

ORF Insert Sequence:

ATGCTGCTCA CTCTTATCAT TCTGTTGCCA GTAGTTTCAA AATTTAGTTT TGTTAGTCTC 
TCAGCACCGC AGCACTGGAG CTGTCCTGAA GGTACTCTCG CAGGAAATGG GAATTCTACT 
TGTGTGGTTT GGTCTCTTTC TTCCCACCCA GGTCCTGCAC CCTTCTTAAT TTTCTCCCAT 
GGAAATAGTA TCTTTAGGAT TGACACAGAA GGAACCAATT ATGAGCAATT GGTGGTGGAT 
GCTGGTGTCT CAGTGATCAT GGATTTTCAT TATAATGAGA AAAGAATCTA TTGGGTGGAT 
TTAGAAAGAC AACTTTTGCA AAGAGTTTTT CTGAATGGGT CAAGGCAAGA GAGAGTATGT 
AATATAGAGA AAAATGTTTC TGGAATGGCA ATAAATTGGA TAAATGAAGA AGTTATTTGG 
TCAAATCAAC AGGAAGGAAT CATTACAGTA ACAGATATGA AAGGAAATAA TTCCCACATT 
CTTTTAAGTG CTTTAAAATA TCCTGCAAAT GTAGCAGTTG ATCCAGTAGA AAGGTTTATA 
TTTTGGTCTT CAGAGGTGGC TGGAAGCCTT TATAGAGCAG ATCTCGATGG TGTGGGAGTG 
AAGGCTCTGT TGGAGACATC AGAGAAAATA ACAGCTGTGT CATTGGATGT GCTTGATAAG 
CGGCTGTTTT GGATTCAGTA CAACAGAGAA GGAAGCAATT CTCTTATTTG CTCCTGTGAT 
TATGATGGAG GTTCTGTCCA CATTAGTAAA CATCCAACAC AGCATAATTT GTTTGCAATG 
TCCCTTTTTG GTGACCGTAT CTTCTATTCA ACATGGAAAA TGAAGACAAT TTGGATAGCC 
AACAAACACA CTGGAAAGGA CATGGTTAGA ATTAACCTCC ATTCATCATT TGTACCACTT 
GGTGAACTGA AAGTAGTGCA TCCACTTGCA CAACCCAAGG CAGAAGATGA CACTTGGGAG 
CCTGAGCAGA AACTTTGCAA ATTGAGGAAA GGAAACTGCA GCAGCACTGT GTGTGGGCAA 
GACCTCCAGT CACACTTGTG CATGTGTGCA GAGGGATACG CCCTAAGTCG AGACCGGAAG 
TACTGTGAAG ATGTTAATGA ATGTGCTTTT TGGAATCATG GCTGTACTCT TGGGTGTAAA 
AACACCCCTG GATCCTATTA CTGCACGTGC CCTGTAGGAT TTGTTCTGCT TCCTGATGGG 
AAACGATGTC ATCAACTTGT TTCCTGTCCA CGCAATGTGT CTGAATGCAG CCATGACTGT 
GTTCTGACAT CAGAAGGTCC CTTATGTTTC TGTCCTGAAG GCTCAGTGCT TGAGAGAGAT 
GGGAAAACAT GTAGCGGTTG TTCCTCACCC GATAATGGTG GATGTAGCCA GCTCTGCGTT 
CCTCTTAGCC CAGTATCCTG GGAATGTGAT TGCTTTCCTG GGTATGACCT ACAACTGGAT 
GAAAAAAGCT GTGCAGCTTC AGGACCACAA CCATTTTTGC TGTTTGCCAA TTCTCAAGAT 
ATTCGACACA TGCATTTTGA TGGAACAGAC TATGGAACTC TGCTCAGCCA GCAGATGGGA 
ATGGTTTATG CCCTAGATCA TGACCCTGTG GAAAATAAGA TATACTTTGC CCATACAGCC 
CTGAAGTGGA TAGAGAGAGC TAATATGGAT GGTTCCCAGC GAGAAAGGCT TATTGAGGAA 
GGAGTAGATG TGCCAGAAGG TCTTGCTGTG GACTGGATTG GCCGTAGATT CTATTGGACA 
GACAGAGGGA AATCTCTGAT TGGAAGGAGT GATTTAAATG GGAAACGTTC CAAAATAATC 
ACTAAGGAGA ACATCTCTCA ACCACGAGGA ATTGCTGTTC ATCCAATGGC CAAGAGATTA 
TTCTGGACTG ATACAGGGAT TAATCCACGA ATTGAAAGTT CTTCCCTCCA AGGCCTTGGC 
CGTCTGGTTA TAGCCAGCTC TGATCTAATC TGGCCCAGTG GAATAACGAT TGACTTCTTA 
ACTGACAAGT TGTACTGGTG CGATGCCAAG CAGTCTGTGA TTGAAATGGC CAATCTGGAT 
GGTTCAAAAC GCCGAAGACT TACCCAGAAT GATGTAGGTC ACCCATTTGC TGTAGCAGTG 
TTTGAGGATT ATGTGTGGTT CTCAGATTGG GCTATGCCAT CAGTAATGAG AGTAAACAAG 
AGGACTGGCA AAGATAGAGT ACGTCTCCAA GGCAGCATGC TGAAGCCCTC ATCACTGGTT 
GTGGTTCATC CATTGGCAAA ACCAGGAGCA GATCCCTGCT TATATCAAAA CGGAGGCTGT 
GAACATATTT GCAAAAAGAG GCTTGGAACT GCTTGGTGTT CGTGTCGTGA AGGTTTTATG 
AAAGCCTCAG ATGGGAAAAC GTGTCTGGCT CTGGATGGTC ATCAGCTGTT GGCAGGTGGT 
GAAGTTGATC TAAAGAACCA AGTAACACCA TTGGACATCT TGTCCAAGAC TAGAGTGTCA 
GAAGATAACA TTACAGAATC TCAACACATG CTAGTGGCTG AAATCATGGT GTCAGATCAA 
GATGACTGTG CTCCTGTGGG ATGCAGCATG TATGCTCGGT GTATTTCAGA GGGAGAGGAT 
GCCACATGTC AGTGTTTGAA AGGATTTGCT GGGGATGGAA AACTATGTTC TGATATAGAT 
GAATGTGAGA TGGGTGTCCC AGTGTGCCCC CCTGCCTCCT CCAAGTGCAT CAACACCGAA 
GGTGGTTATG TCTGCCGGTG CTCAGAAGGC TACCAAGGAG ATGGGATTCA CTGTCTTGAT 
ATTGATGAGT GCCAACTGGG GGAGCACAGC TGTGGAGAGA ATGCCAGCTG CACAAATACA 
GAGGGAGGCT ATACCTGCAT GTGTGCTGGA CGCCTGTCTG AACCAGGACT GATTTGCCCT 
GACTCTACTC CACCCCCTCA CCTCAGGGAA GATGACCACC ACTATTCCGT AAGAAATAGT 
GACTCTGAAT GTCCCCTGTC CCACGATGGG TACTGCCTCC ATGATGGTGT GTGCATGTAT 
ATTGAAGCAT TGGACAAGTA TGCATGCAAC TGTGTTGTTG GCTACATCGG GGAGCGATGT 
CAGTACCGAG ACCTGAAGTG GTGGGAACTG CGCCACGCTG GCCACGGGCA GCAGCAGAAG 
GTCATCGTGG TGGCTGTCTG CGTGGTGGTG CTTGTCATGC TGCTCCTCCT GAGCCTGTGG 
GGGGCCCACT ACTACAGGAC TCAGAAGCTG CTATCGAAAA ACCCAAAGAA TCCTTATGAG 
GAGTCGAGCA GAGATGTGAG GAGTCGCAGG CCTGCTGACA CTGAGGATGG GATGTCCTCT 
TGCCCTCAAC CTTGGTTTGT GGTTATAAAA GAACACCAAG ACCTCAAGAA TGGGGGTCAA 
CCAGTGGCTG GTGAGGATGG CCAGGCAGCA GATGGGTCAA TGCAACCAAC TTCATGGAGG 
CAGGAGCCCC AGTTATGTGG AATGGGCACA GAGCAAGGCT GCTGGATTCC AGTATCCAGT 
GATAAGGGCT CCTGTCCCCA GGTAATGGAG CGAAGCTTTC ATATGCCCTC CTATGGGACA 
CAGACCCTTG AAGGGGGTGT CGAGAAGCCC CATTCTCTCC TATCAGCTAA CCCATTATGG 
CAACAAAGGG CCCTGGACCC ACCACACCAA ATGGAGCTGA CTCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EGF ( XM_017007847.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016863336.1 Homo sapiens epidermal growth factor (EGF), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OHu94952
Clone ID Related Accession (Same CDS sequence)	XM_017007847.1
Accession Version	XM_017007847.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3549bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	pro-epidermal growth factor isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCTCA CTCTTATCAT TCTGTTGCCA GTAGTTTCAA AATTTAGTTT TGTTAGTCTC 
TCAGCACCGC AGCACTGGAG CTGTCCTGAA GGTACTCTCG CAGGAAATGG GAATTCTACT 
TGTGTGGTTT GGTCTCTTTC TTCCCACCCA GGTCCTGCAC CCTTCTTAAT TTTCTCCCAT 
GGAAATAGTA TCTTTAGGAT TGACACAGAA GGAACCAATT ATGAGCAATT GGTGGTGGAT 
GCTGGTGTCT CAGTGATCAT GGATTTTCAT TATAATGAGA AAAGAATCTA TTGGGTGGAT 
TTAGAAAGAC AACTTTTGCA AAGAGTTTTT CTGAATGGGT CAAGGCAAGA GAGAGTATGT 
AATATAGAGA AAAATGTTTC TGGAATGGCA ATAAATTGGA TAAATGAAGA AGTTATTTGG 
TCAAATCAAC AGGAAGGAAT CATTACAGTA ACAGATATGA AAGGAAATAA TTCCCACATT 
CTTTTAAGTG CTTTAAAATA TCCTGCAAAT GTAGCAGTTG ATCCAGTAGA AAGGTTTATA 
TTTTGGTCTT CAGAGGTGGC TGGAAGCCTT TATAGAGCAG ATCTCGATGG TGTGGGAGTG 
AAGGCTCTGT TGGAGACATC AGAGAAAATA ACAGCTGTGT CATTGGATGT GCTTGATAAG 
CGGCTGTTTT GGATTCAGTA CAACAGAGAA GGAAGCAATT CTCTTATTTG CTCCTGTGAT 
TATGATGGAG GTTCTGTCCA CATTAGTAAA CATCCAACAC AGCATAATTT GTTTGCAATG 
TCCCTTTTTG GTGACCGTAT CTTCTATTCA ACATGGAAAA TGAAGACAAT TTGGATAGCC 
AACAAACACA CTGGAAAGGA CATGGTTAGA ATTAACCTCC ATTCATCATT TGTACCACTT 
GGTGAACTGA AAGTAGTGCA TCCACTTGCA CAACCCAAGG CAGAAGATGA CACTTGGGAG 
CCTGAGCAGA AACTTTGCAA ATTGAGGAAA GGAAACTGCA GCAGCACTGT GTGTGGGCAA 
GACCTCCAGT CACACTTGTG CATGTGTGCA GAGGGATACG CCCTAAGTCG AGACCGGAAG 
TACTGTGAAG ATGTTAATGA ATGTGCTTTT TGGAATCATG GCTGTACTCT TGGGTGTAAA 
AACACCCCTG GATCCTATTA CTGCACGTGC CCTGTAGGAT TTGTTCTGCT TCCTGATGGG 
AAACGATGTC ATCAACTTGT TTCCTGTCCA CGCAATGTGT CTGAATGCAG CCATGACTGT 
GTTCTGACAT CAGAAGGTCC CTTATGTTTC TGTCCTGAAG GCTCAGTGCT TGAGAGAGAT 
GGGAAAACAT GTAGCGGTTG TTCCTCACCC GATAATGGTG GATGTAGCCA GCTCTGCGTT 
CCTCTTAGCC CAGTATCCTG GGAATGTGAT TGCTTTCCTG GGTATGACCT ACAACTGGAT 
GAAAAAAGCT GTGCAGCTTC AGGACCACAA CCATTTTTGC TGTTTGCCAA TTCTCAAGAT 
ATTCGACACA TGCATTTTGA TGGAACAGAC TATGGAACTC TGCTCAGCCA GCAGATGGGA 
ATGGTTTATG CCCTAGATCA TGACCCTGTG GAAAATAAGA TATACTTTGC CCATACAGCC 
CTGAAGTGGA TAGAGAGAGC TAATATGGAT GGTTCCCAGC GAGAAAGGCT TATTGAGGAA 
GGAGTAGATG TGCCAGAAGG TCTTGCTGTG GACTGGATTG GCCGTAGATT CTATTGGACA 
GACAGAGGGA AATCTCTGAT TGGAAGGAGT GATTTAAATG GGAAACGTTC CAAAATAATC 
ACTAAGGAGA ACATCTCTCA ACCACGAGGA ATTGCTGTTC ATCCAATGGC CAAGAGATTA 
TTCTGGACTG ATACAGGGAT TAATCCACGA ATTGAAAGTT CTTCCCTCCA AGGCCTTGGC 
CGTCTGGTTA TAGCCAGCTC TGATCTAATC TGGCCCAGTG GAATAACGAT TGACTTCTTA 
ACTGACAAGT TGTACTGGTG CGATGCCAAG CAGTCTGTGA TTGAAATGGC CAATCTGGAT 
GGTTCAAAAC GCCGAAGACT TACCCAGAAT GATGTAGGTC ACCCATTTGC TGTAGCAGTG 
TTTGAGGATT ATGTGTGGTT CTCAGATTGG GCTATGCCAT CAGTAATGAG AGTAAACAAG 
AGGACTGGCA AAGATAGAGT ACGTCTCCAA GGCAGCATGC TGAAGCCCTC ATCACTGGTT 
GTGGTTCATC CATTGGCAAA ACCAGGAGCA GATCCCTGCT TATATCAAAA CGGAGGCTGT 
GAACATATTT GCAAAAAGAG GCTTGGAACT GCTTGGTGTT CGTGTCGTGA AGGTTTTATG 
AAAGCCTCAG ATGGGAAAAC GTGTCTGGCT CTGGATGGTC ATCAGCTGTT GGCAGGTGGT 
GAAGTTGATC TAAAGAACCA AGTAACACCA TTGGACATCT TGTCCAAGAC TAGAGTGTCA 
GAAGATAACA TTACAGAATC TCAACACATG CTAGTGGCTG AAATCATGGT GTCAGATCAA 
GATGACTGTG CTCCTGTGGG ATGCAGCATG TATGCTCGGT GTATTTCAGA GGGAGAGGAT 
GCCACATGTC AGTGTTTGAA AGGATTTGCT GGGGATGGAA AACTATGTTC TGATATAGAT 
GAATGTGAGA TGGGTGTCCC AGTGTGCCCC CCTGCCTCCT CCAAGTGCAT CAACACCGAA 
GGTGGTTATG TCTGCCGGTG CTCAGAAGGC TACCAAGGAG ATGGGATTCA CTGTCTTGAC 
TCTACTCCAC CCCCTCACCT CAGGGAAGAT GACCACCACT ATTCCGTAAG AAATAGTGAC 
TCTGAATGTC CCCTGTCCCA CGATGGGTAC TGCCTCCATG ATGGTGTGTG CATGTATATT 
GAAGCATTGG ACAAGTATGC ATGCAACTGT GTTGTTGGCT ACATCGGGGA GCGATGTCAG 
TACCGAGACC TGAAGTGGTG GGAACTGCGC CACGCTGGCC ACGGGCAGCA GCAGAAGGTC 
ATCGTGGTGG CTGTCTGCGT GGTGGTGCTT GTCATGCTGC TCCTCCTGAG CCTGTGGGGG 
GCCCACTACT ACAGGACTCA GAAGCTGCTA TCGAAAAACC CAAAGAATCC TTATGAGGAG 
TCGAGCAGAG ATGTGAGGAG TCGCAGGCCT GCTGACACTG AGGATGGGAT GTCCTCTTGC 
CCTCAACCTT GGTTTGTGGT TATAAAAGAA CACCAAGACC TCAAGAATGG GGGTCAACCA 
GTGGCTGGTG AGGATGGCCA GGCAGCAGAT GGTCAGTTTT TATCCCTGGC TCCTGGGTCA 
ATGCAACCAA CTTCATGGAG GCAGGAGCCC CAGTTATGTG GAATGGGCAC AGAGCAAGGC 
TGCTGGATTC CAGTATCCAG TGATAAGGGC TCCTGTCCCC AGGTAATGGA GCGAAGCTTT 
CATATGCCCT CCTATGGGAC ACAGACCCTT GAAGGGGGTG TCGAGAAGCC CCATTCTCTC 
CTATCAGCTA ACCCATTATG GCAACAAAGG GCCCTGGACC CACCACACCA AATGGAGCTG 
ACTCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016863336.1
CDS	454..4002
Translation	MLLTLIILLPVVSKFSFVSLSAPQHWSCPEGTLAGNGNSTCVVWSLSSHPGPAPFLIFSHGNSIFRIDTEGTNYEQLVVDAGVSVIMDFHYNEKRIYWVDLERQLLQRVFLNGSRQERVCNIEKNVSGMAINWINEEVIWSNQQEGIITVTDMKGNNSHILLSALKYPANVAVDPVERFIFWSSEVAGSLYRADLDGVGVKALLETSEKITAVSLDVLDKRLFWIQYNREGSNSLICSCDYDGGSVHISKHPTQHNLFAMSLFGDRIFYSTWKMKTIWIANKHTGKDMVRINLHSSFVPLGELKVVHPLAQPKAEDDTWEPEQKLCKLRKGNCSSTVCGQDLQSHLCMCAEGYALSRDRKYCEDVNECAFWNHGCTLGCKNTPGSYYCTCPVGFVLLPDGKRCHQLVSCPRNVSECSHDCVLTSEGPLCFCPEGSVLERDGKTCSGCSSPDNGGCSQLCVPLSPVSWECDCFPGYDLQLDEKSCAASGPQPFLLFANSQDIRHMHFDGTDYGTLLSQQMGMVYALDHDPVENKIYFAHTALKWIERANMDGSQRERLIEEGVDVPEGLAVDWIGRRFYWTDRGKSLIGRSDLNGKRSKIITKENISQPRGIAVHPMAKRLFWTDTGINPRIESSSLQGLGRLVIASSDLIWPSGITIDFLTDKLYWCDAKQSVIEMANLDGSKRRRLTQNDVGHPFAVAVFEDYVWFSDWAMPSVMRVNKRTGKDRVRLQGSMLKPSSLVVVHPLAKPGADPCLYQNGGCEHICKKRLGTAWCSCREGFMKASDGKTCLALDGHQLLAGGEVDLKNQVTPLDILSKTRVSEDNITESQHMLVAEIMVSDQDDCAPVGCSMYARCISEGEDATCQCLKGFAGDGKLCSDIDECEMGVPVCPPASSKCINTEGGYVCRCSEGYQGDGIHCLDSTPPPHLREDDHHYSVRNSDSECPLSHDGYCLHDGVCMYIEALDKYACNCVVGYIGERCQYRDLKWWELRHAGHGQQQKVIVVAVCVVVLVMLLLLSLWGAHYYRTQKLLSKNPKNPYEESSRDVRSRRPADTEDGMSSCPQPWFVVIKEHQDLKNGGQPVAGEDGQAADGQFLSLAPGSMQPTSWRQEPQLCGMGTEQGCWIPVSSDKGSCPQVMERSFHMPSYGTQTLEGGVEKPHSLLSANPLWQQRALDPPHQMELTQ

Target ORF information:

RefSeq Version	XM_017007847.1
Organism	Homo sapiens(human)
Definition	Homo sapiens epidermal growth factor (EGF), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017007847.1

ORF Insert Sequence:

ATGCTGCTCA CTCTTATCAT TCTGTTGCCA GTAGTTTCAA AATTTAGTTT TGTTAGTCTC 
TCAGCACCGC AGCACTGGAG CTGTCCTGAA GGTACTCTCG CAGGAAATGG GAATTCTACT 
TGTGTGGTTT GGTCTCTTTC TTCCCACCCA GGTCCTGCAC CCTTCTTAAT TTTCTCCCAT 
GGAAATAGTA TCTTTAGGAT TGACACAGAA GGAACCAATT ATGAGCAATT GGTGGTGGAT 
GCTGGTGTCT CAGTGATCAT GGATTTTCAT TATAATGAGA AAAGAATCTA TTGGGTGGAT 
TTAGAAAGAC AACTTTTGCA AAGAGTTTTT CTGAATGGGT CAAGGCAAGA GAGAGTATGT 
AATATAGAGA AAAATGTTTC TGGAATGGCA ATAAATTGGA TAAATGAAGA AGTTATTTGG 
TCAAATCAAC AGGAAGGAAT CATTACAGTA ACAGATATGA AAGGAAATAA TTCCCACATT 
CTTTTAAGTG CTTTAAAATA TCCTGCAAAT GTAGCAGTTG ATCCAGTAGA AAGGTTTATA 
TTTTGGTCTT CAGAGGTGGC TGGAAGCCTT TATAGAGCAG ATCTCGATGG TGTGGGAGTG 
AAGGCTCTGT TGGAGACATC AGAGAAAATA ACAGCTGTGT CATTGGATGT GCTTGATAAG 
CGGCTGTTTT GGATTCAGTA CAACAGAGAA GGAAGCAATT CTCTTATTTG CTCCTGTGAT 
TATGATGGAG GTTCTGTCCA CATTAGTAAA CATCCAACAC AGCATAATTT GTTTGCAATG 
TCCCTTTTTG GTGACCGTAT CTTCTATTCA ACATGGAAAA TGAAGACAAT TTGGATAGCC 
AACAAACACA CTGGAAAGGA CATGGTTAGA ATTAACCTCC ATTCATCATT TGTACCACTT 
GGTGAACTGA AAGTAGTGCA TCCACTTGCA CAACCCAAGG CAGAAGATGA CACTTGGGAG 
CCTGAGCAGA AACTTTGCAA ATTGAGGAAA GGAAACTGCA GCAGCACTGT GTGTGGGCAA 
GACCTCCAGT CACACTTGTG CATGTGTGCA GAGGGATACG CCCTAAGTCG AGACCGGAAG 
TACTGTGAAG ATGTTAATGA ATGTGCTTTT TGGAATCATG GCTGTACTCT TGGGTGTAAA 
AACACCCCTG GATCCTATTA CTGCACGTGC CCTGTAGGAT TTGTTCTGCT TCCTGATGGG 
AAACGATGTC ATCAACTTGT TTCCTGTCCA CGCAATGTGT CTGAATGCAG CCATGACTGT 
GTTCTGACAT CAGAAGGTCC CTTATGTTTC TGTCCTGAAG GCTCAGTGCT TGAGAGAGAT 
GGGAAAACAT GTAGCGGTTG TTCCTCACCC GATAATGGTG GATGTAGCCA GCTCTGCGTT 
CCTCTTAGCC CAGTATCCTG GGAATGTGAT TGCTTTCCTG GGTATGACCT ACAACTGGAT 
GAAAAAAGCT GTGCAGCTTC AGGACCACAA CCATTTTTGC TGTTTGCCAA TTCTCAAGAT 
ATTCGACACA TGCATTTTGA TGGAACAGAC TATGGAACTC TGCTCAGCCA GCAGATGGGA 
ATGGTTTATG CCCTAGATCA TGACCCTGTG GAAAATAAGA TATACTTTGC CCATACAGCC 
CTGAAGTGGA TAGAGAGAGC TAATATGGAT GGTTCCCAGC GAGAAAGGCT TATTGAGGAA 
GGAGTAGATG TGCCAGAAGG TCTTGCTGTG GACTGGATTG GCCGTAGATT CTATTGGACA 
GACAGAGGGA AATCTCTGAT TGGAAGGAGT GATTTAAATG GGAAACGTTC CAAAATAATC 
ACTAAGGAGA ACATCTCTCA ACCACGAGGA ATTGCTGTTC ATCCAATGGC CAAGAGATTA 
TTCTGGACTG ATACAGGGAT TAATCCACGA ATTGAAAGTT CTTCCCTCCA AGGCCTTGGC 
CGTCTGGTTA TAGCCAGCTC TGATCTAATC TGGCCCAGTG GAATAACGAT TGACTTCTTA 
ACTGACAAGT TGTACTGGTG CGATGCCAAG CAGTCTGTGA TTGAAATGGC CAATCTGGAT 
GGTTCAAAAC GCCGAAGACT TACCCAGAAT GATGTAGGTC ACCCATTTGC TGTAGCAGTG 
TTTGAGGATT ATGTGTGGTT CTCAGATTGG GCTATGCCAT CAGTAATGAG AGTAAACAAG 
AGGACTGGCA AAGATAGAGT ACGTCTCCAA GGCAGCATGC TGAAGCCCTC ATCACTGGTT 
GTGGTTCATC CATTGGCAAA ACCAGGAGCA GATCCCTGCT TATATCAAAA CGGAGGCTGT 
GAACATATTT GCAAAAAGAG GCTTGGAACT GCTTGGTGTT CGTGTCGTGA AGGTTTTATG 
AAAGCCTCAG ATGGGAAAAC GTGTCTGGCT CTGGATGGTC ATCAGCTGTT GGCAGGTGGT 
GAAGTTGATC TAAAGAACCA AGTAACACCA TTGGACATCT TGTCCAAGAC TAGAGTGTCA 
GAAGATAACA TTACAGAATC TCAACACATG CTAGTGGCTG AAATCATGGT GTCAGATCAA 
GATGACTGTG CTCCTGTGGG ATGCAGCATG TATGCTCGGT GTATTTCAGA GGGAGAGGAT 
GCCACATGTC AGTGTTTGAA AGGATTTGCT GGGGATGGAA AACTATGTTC TGATATAGAT 
GAATGTGAGA TGGGTGTCCC AGTGTGCCCC CCTGCCTCCT CCAAGTGCAT CAACACCGAA 
GGTGGTTATG TCTGCCGGTG CTCAGAAGGC TACCAAGGAG ATGGGATTCA CTGTCTTGAC 
TCTACTCCAC CCCCTCACCT CAGGGAAGAT GACCACCACT ATTCCGTAAG AAATAGTGAC 
TCTGAATGTC CCCTGTCCCA CGATGGGTAC TGCCTCCATG ATGGTGTGTG CATGTATATT 
GAAGCATTGG ACAAGTATGC ATGCAACTGT GTTGTTGGCT ACATCGGGGA GCGATGTCAG 
TACCGAGACC TGAAGTGGTG GGAACTGCGC CACGCTGGCC ACGGGCAGCA GCAGAAGGTC 
ATCGTGGTGG CTGTCTGCGT GGTGGTGCTT GTCATGCTGC TCCTCCTGAG CCTGTGGGGG 
GCCCACTACT ACAGGACTCA GAAGCTGCTA TCGAAAAACC CAAAGAATCC TTATGAGGAG 
TCGAGCAGAG ATGTGAGGAG TCGCAGGCCT GCTGACACTG AGGATGGGAT GTCCTCTTGC 
CCTCAACCTT GGTTTGTGGT TATAAAAGAA CACCAAGACC TCAAGAATGG GGGTCAACCA 
GTGGCTGGTG AGGATGGCCA GGCAGCAGAT GGTCAGTTTT TATCCCTGGC TCCTGGGTCA 
ATGCAACCAA CTTCATGGAG GCAGGAGCCC CAGTTATGTG GAATGGGCAC AGAGCAAGGC 
TGCTGGATTC CAGTATCCAG TGATAAGGGC TCCTGTCCCC AGGTAATGGA GCGAAGCTTT 
CATATGCCCT CCTATGGGAC ACAGACCCTT GAAGGGGGTG TCGAGAAGCC CCATTCTCTC 
CTATCAGCTA ACCCATTATG GCAACAAAGG GCCCTGGACC CACCACACCA AATGGAGCTG 
ACTCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EGF ( XM_017007848.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016863337.1 Homo sapiens epidermal growth factor (EGF), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OHu94953
Clone ID Related Accession (Same CDS sequence)	XM_017007848.1
Accession Version	XM_017007848.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3546bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	pro-epidermal growth factor isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCTCA CTCTTATCAT TCTGTTGCCA GTAGTTTCAA AATTTAGTTT TGTTAGTCTC 
TCAGCACCGC AGCACTGGAG CTGTCCTGAA GGTACTCTCG CAGGAAATGG GAATTCTACT 
TGTGTGGTTT GGTCTCTTTC TTCCCACCCA GGTCCTGCAC CCTTCTTAAT TTTCTCCCAT 
GGAAATAGTA TCTTTAGGAT TGACACAGAA GGAACCAATT ATGAGCAATT GGTGGTGGAT 
GCTGGTGTCT CAGTGATCAT GGATTTTCAT TATAATGAGA AAAGAATCTA TTGGGTGGAT 
TTAGAAAGAC AACTTTTGCA AAGAGTTTTT CTGAATGGGT CAAGGCAAGA GAGAGTATGT 
AATATAGAGA AAAATGTTTC TGGAATGGCA ATAAATTGGA TAAATGAAGA AGTTATTTGG 
TCAAATCAAC AGGAAGGAAT CATTACAGTA ACAGATATGA AAGGAAATAA TTCCCACATT 
CTTTTAAGTG CTTTAAAATA TCCTGCAAAT GTAGCAGTTG ATCCAGTAGA AAGGTTTATA 
TTTTGGTCTT CAGAGGTGGC TGGAAGCCTT TATAGAGCAG ATCTCGATGG TGTGGGAGTG 
AAGGCTCTGT TGGAGACATC AGAGAAAATA ACAGCTGTGT CATTGGATGT GCTTGATAAG 
CGGCTGTTTT GGATTCAGTA CAACAGAGAA GGAAGCAATT CTCTTATTTG CTCCTGTGAT 
TATGATGGAG GTTCTGTCCA CATTAGTAAA CATCCAACAC AGCATAATTT GTTTGCAATG 
TCCCTTTTTG GTGACCGTAT CTTCTATTCA ACATGGAAAA TGAAGACAAT TTGGATAGCC 
AACAAACACA CTGGAAAGGA CATGGTTAGA ATTAACCTCC ATTCATCATT TGTACCACTT 
GGTGAACTGA AAGTAGTGCA TCCACTTGCA CAACCCAAGG CAGAAGATGA CACTTGGGAG 
CCTGATGTTA ATGAATGTGC TTTTTGGAAT CATGGCTGTA CTCTTGGGTG TAAAAACACC 
CCTGGATCCT ATTACTGCAC GTGCCCTGTA GGATTTGTTC TGCTTCCTGA TGGGAAACGA 
TGTCATCAAC TTGTTTCCTG TCCACGCAAT GTGTCTGAAT GCAGCCATGA CTGTGTTCTG 
ACATCAGAAG GTCCCTTATG TTTCTGTCCT GAAGGCTCAG TGCTTGAGAG AGATGGGAAA 
ACATGTAGCG GTTGTTCCTC ACCCGATAAT GGTGGATGTA GCCAGCTCTG CGTTCCTCTT 
AGCCCAGTAT CCTGGGAATG TGATTGCTTT CCTGGGTATG ACCTACAACT GGATGAAAAA 
AGCTGTGCAG CTTCAGGACC ACAACCATTT TTGCTGTTTG CCAATTCTCA AGATATTCGA 
CACATGCATT TTGATGGAAC AGACTATGGA ACTCTGCTCA GCCAGCAGAT GGGAATGGTT 
TATGCCCTAG ATCATGACCC TGTGGAAAAT AAGATATACT TTGCCCATAC AGCCCTGAAG 
TGGATAGAGA GAGCTAATAT GGATGGTTCC CAGCGAGAAA GGCTTATTGA GGAAGGAGTA 
GATGTGCCAG AAGGTCTTGC TGTGGACTGG ATTGGCCGTA GATTCTATTG GACAGACAGA 
GGGAAATCTC TGATTGGAAG GAGTGATTTA AATGGGAAAC GTTCCAAAAT AATCACTAAG 
GAGAACATCT CTCAACCACG AGGAATTGCT GTTCATCCAA TGGCCAAGAG ATTATTCTGG 
ACTGATACAG GGATTAATCC ACGAATTGAA AGTTCTTCCC TCCAAGGCCT TGGCCGTCTG 
GTTATAGCCA GCTCTGATCT AATCTGGCCC AGTGGAATAA CGATTGACTT CTTAACTGAC 
AAGTTGTACT GGTGCGATGC CAAGCAGTCT GTGATTGAAA TGGCCAATCT GGATGGTTCA 
AAACGCCGAA GACTTACCCA GAATGATGTA GGTCACCCAT TTGCTGTAGC AGTGTTTGAG 
GATTATGTGT GGTTCTCAGA TTGGGCTATG CCATCAGTAA TGAGAGTAAA CAAGAGGACT 
GGCAAAGATA GAGTACGTCT CCAAGGCAGC ATGCTGAAGC CCTCATCACT GGTTGTGGTT 
CATCCATTGG CAAAACCAGG AGCAGATCCC TGCTTATATC AAAACGGAGG CTGTGAACAT 
ATTTGCAAAA AGAGGCTTGG AACTGCTTGG TGTTCGTGTC GTGAAGGTTT TATGAAAGCC 
TCAGATGGGA AAACGTGTCT GGCTCTGGAT GGTCATCAGC TGTTGGCAGG TGGTGAAGTT 
GATCTAAAGA ACCAAGTAAC ACCATTGGAC ATCTTGTCCA AGACTAGAGT GTCAGAAGAT 
AACATTACAG AATCTCAACA CATGCTAGTG GCTGAAATCA TGGTGTCAGA TCAAGATGAC 
TGTGCTCCTG TGGGATGCAG CATGTATGCT CGGTGTATTT CAGAGGGAGA GGATGCCACA 
TGTCAGTGTT TGAAAGGATT TGCTGGGGAT GGAAAACTAT GTTCTGATAT AGATGAATGT 
GAGATGGGTG TCCCAGTGTG CCCCCCTGCC TCCTCCAAGT GCATCAACAC CGAAGGTGGT 
TATGTCTGCC GGTGCTCAGA AGGCTACCAA GGAGATGGGA TTCACTGTCT TGATATTGAT 
GAGTGCCAAC TGGGGGAGCA CAGCTGTGGA GAGAATGCCA GCTGCACAAA TACAGAGGGA 
GGCTATACCT GCATGTGTGC TGGACGCCTG TCTGAACCAG GACTGATTTG CCCTGACTCT 
ACTCCACCCC CTCACCTCAG GGAAGATGAC CACCACTATT CCGTAAGAAA TAGTGACTCT 
GAATGTCCCC TGTCCCACGA TGGGTACTGC CTCCATGATG GTGTGTGCAT GTATATTGAA 
GCATTGGACA AGTATGCATG CAACTGTGTT GTTGGCTACA TCGGGGAGCG ATGTCAGTAC 
CGAGACCTGA AGTGGTGGGA ACTGCGCCAC GCTGGCCACG GGCAGCAGCA GAAGGTCATC 
GTGGTGGCTG TCTGCGTGGT GGTGCTTGTC ATGCTGCTCC TCCTGAGCCT GTGGGGGGCC 
CACTACTACA GGACTCAGAA GCTGCTATCG AAAAACCCAA AGAATCCTTA TGAGGAGTCG 
AGCAGAGATG TGAGGAGTCG CAGGCCTGCT GACACTGAGG ATGGGATGTC CTCTTGCCCT 
CAACCTTGGT TTGTGGTTAT AAAAGAACAC CAAGACCTCA AGAATGGGGG TCAACCAGTG 
GCTGGTGAGG ATGGCCAGGC AGCAGATGGT CAGTTTTTAT CCCTGGCTCC TGGGTCAATG 
CAACCAACTT CATGGAGGCA GGAGCCCCAG TTATGTGGAA TGGGCACAGA GCAAGGCTGC 
TGGATTCCAG TATCCAGTGA TAAGGGCTCC TGTCCCCAGG TAATGGAGCG AAGCTTTCAT 
ATGCCCTCCT ATGGGACACA GACCCTTGAA GGGGGTGTCG AGAAGCCCCA TTCTCTCCTA 
TCAGCTAACC CATTATGGCA ACAAAGGGCC CTGGACCCAC CACACCAAAT GGAGCTGACT 
CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016863337.1
CDS	454..3999
Translation	MLLTLIILLPVVSKFSFVSLSAPQHWSCPEGTLAGNGNSTCVVWSLSSHPGPAPFLIFSHGNSIFRIDTEGTNYEQLVVDAGVSVIMDFHYNEKRIYWVDLERQLLQRVFLNGSRQERVCNIEKNVSGMAINWINEEVIWSNQQEGIITVTDMKGNNSHILLSALKYPANVAVDPVERFIFWSSEVAGSLYRADLDGVGVKALLETSEKITAVSLDVLDKRLFWIQYNREGSNSLICSCDYDGGSVHISKHPTQHNLFAMSLFGDRIFYSTWKMKTIWIANKHTGKDMVRINLHSSFVPLGELKVVHPLAQPKAEDDTWEPDVNECAFWNHGCTLGCKNTPGSYYCTCPVGFVLLPDGKRCHQLVSCPRNVSECSHDCVLTSEGPLCFCPEGSVLERDGKTCSGCSSPDNGGCSQLCVPLSPVSWECDCFPGYDLQLDEKSCAASGPQPFLLFANSQDIRHMHFDGTDYGTLLSQQMGMVYALDHDPVENKIYFAHTALKWIERANMDGSQRERLIEEGVDVPEGLAVDWIGRRFYWTDRGKSLIGRSDLNGKRSKIITKENISQPRGIAVHPMAKRLFWTDTGINPRIESSSLQGLGRLVIASSDLIWPSGITIDFLTDKLYWCDAKQSVIEMANLDGSKRRRLTQNDVGHPFAVAVFEDYVWFSDWAMPSVMRVNKRTGKDRVRLQGSMLKPSSLVVVHPLAKPGADPCLYQNGGCEHICKKRLGTAWCSCREGFMKASDGKTCLALDGHQLLAGGEVDLKNQVTPLDILSKTRVSEDNITESQHMLVAEIMVSDQDDCAPVGCSMYARCISEGEDATCQCLKGFAGDGKLCSDIDECEMGVPVCPPASSKCINTEGGYVCRCSEGYQGDGIHCLDIDECQLGEHSCGENASCTNTEGGYTCMCAGRLSEPGLICPDSTPPPHLREDDHHYSVRNSDSECPLSHDGYCLHDGVCMYIEALDKYACNCVVGYIGERCQYRDLKWWELRHAGHGQQQKVIVVAVCVVVLVMLLLLSLWGAHYYRTQKLLSKNPKNPYEESSRDVRSRRPADTEDGMSSCPQPWFVVIKEHQDLKNGGQPVAGEDGQAADGQFLSLAPGSMQPTSWRQEPQLCGMGTEQGCWIPVSSDKGSCPQVMERSFHMPSYGTQTLEGGVEKPHSLLSANPLWQQRALDPPHQMELTQ

Target ORF information:

RefSeq Version	XM_017007848.1
Organism	Homo sapiens(human)
Definition	Homo sapiens epidermal growth factor (EGF), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017007848.1

ORF Insert Sequence:

ATGCTGCTCA CTCTTATCAT TCTGTTGCCA GTAGTTTCAA AATTTAGTTT TGTTAGTCTC 
TCAGCACCGC AGCACTGGAG CTGTCCTGAA GGTACTCTCG CAGGAAATGG GAATTCTACT 
TGTGTGGTTT GGTCTCTTTC TTCCCACCCA GGTCCTGCAC CCTTCTTAAT TTTCTCCCAT 
GGAAATAGTA TCTTTAGGAT TGACACAGAA GGAACCAATT ATGAGCAATT GGTGGTGGAT 
GCTGGTGTCT CAGTGATCAT GGATTTTCAT TATAATGAGA AAAGAATCTA TTGGGTGGAT 
TTAGAAAGAC AACTTTTGCA AAGAGTTTTT CTGAATGGGT CAAGGCAAGA GAGAGTATGT 
AATATAGAGA AAAATGTTTC TGGAATGGCA ATAAATTGGA TAAATGAAGA AGTTATTTGG 
TCAAATCAAC AGGAAGGAAT CATTACAGTA ACAGATATGA AAGGAAATAA TTCCCACATT 
CTTTTAAGTG CTTTAAAATA TCCTGCAAAT GTAGCAGTTG ATCCAGTAGA AAGGTTTATA 
TTTTGGTCTT CAGAGGTGGC TGGAAGCCTT TATAGAGCAG ATCTCGATGG TGTGGGAGTG 
AAGGCTCTGT TGGAGACATC AGAGAAAATA ACAGCTGTGT CATTGGATGT GCTTGATAAG 
CGGCTGTTTT GGATTCAGTA CAACAGAGAA GGAAGCAATT CTCTTATTTG CTCCTGTGAT 
TATGATGGAG GTTCTGTCCA CATTAGTAAA CATCCAACAC AGCATAATTT GTTTGCAATG 
TCCCTTTTTG GTGACCGTAT CTTCTATTCA ACATGGAAAA TGAAGACAAT TTGGATAGCC 
AACAAACACA CTGGAAAGGA CATGGTTAGA ATTAACCTCC ATTCATCATT TGTACCACTT 
GGTGAACTGA AAGTAGTGCA TCCACTTGCA CAACCCAAGG CAGAAGATGA CACTTGGGAG 
CCTGATGTTA ATGAATGTGC TTTTTGGAAT CATGGCTGTA CTCTTGGGTG TAAAAACACC 
CCTGGATCCT ATTACTGCAC GTGCCCTGTA GGATTTGTTC TGCTTCCTGA TGGGAAACGA 
TGTCATCAAC TTGTTTCCTG TCCACGCAAT GTGTCTGAAT GCAGCCATGA CTGTGTTCTG 
ACATCAGAAG GTCCCTTATG TTTCTGTCCT GAAGGCTCAG TGCTTGAGAG AGATGGGAAA 
ACATGTAGCG GTTGTTCCTC ACCCGATAAT GGTGGATGTA GCCAGCTCTG CGTTCCTCTT 
AGCCCAGTAT CCTGGGAATG TGATTGCTTT CCTGGGTATG ACCTACAACT GGATGAAAAA 
AGCTGTGCAG CTTCAGGACC ACAACCATTT TTGCTGTTTG CCAATTCTCA AGATATTCGA 
CACATGCATT TTGATGGAAC AGACTATGGA ACTCTGCTCA GCCAGCAGAT GGGAATGGTT 
TATGCCCTAG ATCATGACCC TGTGGAAAAT AAGATATACT TTGCCCATAC AGCCCTGAAG 
TGGATAGAGA GAGCTAATAT GGATGGTTCC CAGCGAGAAA GGCTTATTGA GGAAGGAGTA 
GATGTGCCAG AAGGTCTTGC TGTGGACTGG ATTGGCCGTA GATTCTATTG GACAGACAGA 
GGGAAATCTC TGATTGGAAG GAGTGATTTA AATGGGAAAC GTTCCAAAAT AATCACTAAG 
GAGAACATCT CTCAACCACG AGGAATTGCT GTTCATCCAA TGGCCAAGAG ATTATTCTGG 
ACTGATACAG GGATTAATCC ACGAATTGAA AGTTCTTCCC TCCAAGGCCT TGGCCGTCTG 
GTTATAGCCA GCTCTGATCT AATCTGGCCC AGTGGAATAA CGATTGACTT CTTAACTGAC 
AAGTTGTACT GGTGCGATGC CAAGCAGTCT GTGATTGAAA TGGCCAATCT GGATGGTTCA 
AAACGCCGAA GACTTACCCA GAATGATGTA GGTCACCCAT TTGCTGTAGC AGTGTTTGAG 
GATTATGTGT GGTTCTCAGA TTGGGCTATG CCATCAGTAA TGAGAGTAAA CAAGAGGACT 
GGCAAAGATA GAGTACGTCT CCAAGGCAGC ATGCTGAAGC CCTCATCACT GGTTGTGGTT 
CATCCATTGG CAAAACCAGG AGCAGATCCC TGCTTATATC AAAACGGAGG CTGTGAACAT 
ATTTGCAAAA AGAGGCTTGG AACTGCTTGG TGTTCGTGTC GTGAAGGTTT TATGAAAGCC 
TCAGATGGGA AAACGTGTCT GGCTCTGGAT GGTCATCAGC TGTTGGCAGG TGGTGAAGTT 
GATCTAAAGA ACCAAGTAAC ACCATTGGAC ATCTTGTCCA AGACTAGAGT GTCAGAAGAT 
AACATTACAG AATCTCAACA CATGCTAGTG GCTGAAATCA TGGTGTCAGA TCAAGATGAC 
TGTGCTCCTG TGGGATGCAG CATGTATGCT CGGTGTATTT CAGAGGGAGA GGATGCCACA 
TGTCAGTGTT TGAAAGGATT TGCTGGGGAT GGAAAACTAT GTTCTGATAT AGATGAATGT 
GAGATGGGTG TCCCAGTGTG CCCCCCTGCC TCCTCCAAGT GCATCAACAC CGAAGGTGGT 
TATGTCTGCC GGTGCTCAGA AGGCTACCAA GGAGATGGGA TTCACTGTCT TGATATTGAT 
GAGTGCCAAC TGGGGGAGCA CAGCTGTGGA GAGAATGCCA GCTGCACAAA TACAGAGGGA 
GGCTATACCT GCATGTGTGC TGGACGCCTG TCTGAACCAG GACTGATTTG CCCTGACTCT 
ACTCCACCCC CTCACCTCAG GGAAGATGAC CACCACTATT CCGTAAGAAA TAGTGACTCT 
GAATGTCCCC TGTCCCACGA TGGGTACTGC CTCCATGATG GTGTGTGCAT GTATATTGAA 
GCATTGGACA AGTATGCATG CAACTGTGTT GTTGGCTACA TCGGGGAGCG ATGTCAGTAC 
CGAGACCTGA AGTGGTGGGA ACTGCGCCAC GCTGGCCACG GGCAGCAGCA GAAGGTCATC 
GTGGTGGCTG TCTGCGTGGT GGTGCTTGTC ATGCTGCTCC TCCTGAGCCT GTGGGGGGCC 
CACTACTACA GGACTCAGAA GCTGCTATCG AAAAACCCAA AGAATCCTTA TGAGGAGTCG 
AGCAGAGATG TGAGGAGTCG CAGGCCTGCT GACACTGAGG ATGGGATGTC CTCTTGCCCT 
CAACCTTGGT TTGTGGTTAT AAAAGAACAC CAAGACCTCA AGAATGGGGG TCAACCAGTG 
GCTGGTGAGG ATGGCCAGGC AGCAGATGGT CAGTTTTTAT CCCTGGCTCC TGGGTCAATG 
CAACCAACTT CATGGAGGCA GGAGCCCCAG TTATGTGGAA TGGGCACAGA GCAAGGCTGC 
TGGATTCCAG TATCCAGTGA TAAGGGCTCC TGTCCCCAGG TAATGGAGCG AAGCTTTCAT 
ATGCCCTCCT ATGGGACACA GACCCTTGAA GGGGGTGTCG AGAAGCCCCA TTCTCTCCTA 
TCAGCTAACC CATTATGGCA ACAAAGGGCC CTGGACCCAC CACACCAAAT GGAGCTGACT 
CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EGF ( XM_017007849.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016863338.1 Homo sapiens epidermal growth factor (EGF), transcript variant X7, mRNA.

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CloneID	OHu94954
Clone ID Related Accession (Same CDS sequence)	XM_017007849.1
Accession Version	XM_017007849.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3429bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	pro-epidermal growth factor isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCTCA CTCTTATCAT TCTGTTGCCA GTAGTTTCAA AATTTAGTTT TGTTAGTCTC 
TCAGCACCGC AGCACTGGAG CTGTCCTGAA GGTACTCTCG CAGGAAATGG GAATTCTACT 
TGTGTGGTTT GGTCTCTTTC TTCCCACCCA GGTCCTGCAC CCTTCTTAAT TTTCTCCCAT 
GGAAATAGTA TCTTTAGGAT TGACACAGAA GGAACCAATT ATGAGCAATT GGTGGTGGAT 
GCTGGTGTCT CAGTGATCAT GGATTTTCAT TATAATGAGA AAAGAATCTA TTGGGTGGAT 
TTAGAAAGAC AACTTTTGCA AAGAGTTTTT CTGAATGGGT CAAGGCAAGA GAGAGTATGT 
AATATAGAGA AAAATGTTTC TGGAATGGCA ATAAATTGGA TAAATGAAGA AGTTATTTGG 
TCAAATCAAC AGGAAGGAAT CATTACAGTA ACAGATATGA AAGGAAATAA TTCCCACATT 
CTTTTAAGTG CTTTAAAATA TCCTGCAAAT GTAGCAGTTG ATCCAGTAGA AAGGTTTATA 
TTTTGGTCTT CAGAGGTGGC TGGAAGCCTT TATAGAGCAG ATCTCGATGG TGTGGGAGTG 
AAGGCTCTGT TGGAGACATC AGAGAAAATA ACAGCTGTGT CATTGGATGT GCTTGATAAG 
CGGCTGTTTT GGATTCAGTA CAACAGAGAA GGAAGCAATT CTCTTATTTG CTCCTGTGAT 
TATGATGGAG GTTCTGTCCA CATTAGTAAA CATCCAACAC AGCATAATTT GTTTGCAATG 
TCCCTTTTTG GTGACCGTAT CTTCTATTCA ACATGGAAAA TGAAGACAAT TTGGATAGCC 
AACAAACACA CTGGAAAGGA CATGGTTAGA ATTAACCTCC ATTCATCATT TGTACCACTT 
GGTGAACTGA AAGTAGTGCA TCCACTTGCA CAACCCAAGG CAGAAGATGA CACTTGGGAG 
CCTGAGCAGA AACTTTGCAA ATTGAGGAAA GGAAACTGCA GCAGCACTGT GTGTGGGCAA 
GACCTCCAGT CACACTTGTG CATGTGTGCA GAGGGATACG CCCTAAGTCG AGACCGGAAG 
TACTGTGAAG ATGTTAATGA ATGTGCTTTT TGGAATCATG GCTGTACTCT TGGGTGTAAA 
AACACCCCTG GATCCTATTA CTGCACGTGC CCTGTAGGAT TTGTTCTGCT TCCTGATGGG 
AAACGATGTC ATCAACTTGT TTCCTGTCCA CGCAATGTGT CTGAATGCAG CCATGACTGT 
GTTCTGACAT CAGAAGGTCC CTTATGTTTC TGTCCTGAAG GCTCAGTGCT TGAGAGAGAT 
GGGAAAACAT GTAGCGGTTG TTCCTCACCC GATAATGGTG GATGTAGCCA GCTCTGCGTT 
CCTCTTAGCC CAGTATCCTG GGAATGTGAT TGCTTTCCTG GGTATGACCT ACAACTGGAT 
GAAAAAAGCT GTGCAGCTTC AGGACCACAA CCATTTTTGC TGTTTGCCAA TTCTCAAGAT 
ATTCGACACA TGCATTTTGA TGGAACAGAC TATGGAACTC TGCTCAGCCA GCAGATGGGA 
ATGGTTTATG CCCTAGATCA TGACCCTGTG GAAAATAAGA TATACTTTGC CCATACAGCC 
CTGAAGTGGA TAGAGAGAGC TAATATGGAT GGTTCCCAGC GAGAAAGGCT TATTGAGGAA 
GGAGTAGATG TGCCAGAAGG TCTTGCTGTG GACTGGATTG GCCGTAGATT CTATTGGACA 
GACAGAGGGA AATCTCTGAT TGGAAGGAGT GATTTAAATG GGAAACGTTC CAAAATAATC 
ACTAAGGAGA ACATCTCTCA ACCACGAGGA ATTGCTGTTC ATCCAATGGC CAAGAGATTA 
TTCTGGACTG ATACAGGGAT TAATCCACGA ATTGAAAGTT CTTCCCTCCA AGGCCTTGGC 
CGTCTGGTTA TAGCCAGCTC TGATCTAATC TGGCCCAGTG GAATAACGAT TGACTTCTTA 
ACTGACAAGT TGTACTGGTG CGATGCCAAG CAGTCTGTGA TTGAAATGGC CAATCTGGAT 
GGTTCAAAAC GCCGAAGACT TACCCAGAAT GATGTAGGTC ACCCATTTGC TGTAGCAGTG 
TTTGAGGATT ATGTGTGGTT CTCAGATTGG GCTATGCCAT CAGTAATGAG AGTAAACAAG 
AGGACTGGCA AAGATAGAGT ACGTCTCCAA GGCAGCATGC TGAAGCCCTC ATCACTGGTT 
GTGGTTCATC CATTGGCAAA ACCAGGAGCA GATCCCTGCT TATATCAAAA CGGAGGCTGT 
GAACATATTT GCAAAAAGAG GCTTGGAACT GCTTGGTGTT CGTGTCGTGA AGGTTTTATG 
AAAGCCTCAG ATGGGAAAAC GTGTCTGGCT CTGGATGGTC ATCAGCTGTT GGCAGGTGGT 
GAAGTTGATC TAAAGAACCA AGTAACACCA TTGGACATCT TGTCCAAGAC TAGAGTGTCA 
GAAGATAACA TTACAGAATC TCAACACATG CTAGTGGCTG AAATCATGGT GTCAGATATT 
GATGAGTGCC AACTGGGGGA GCACAGCTGT GGAGAGAATG CCAGCTGCAC AAATACAGAG 
GGAGGCTATA CCTGCATGTG TGCTGGACGC CTGTCTGAAC CAGGACTGAT TTGCCCTGAC 
TCTACTCCAC CCCCTCACCT CAGGGAAGAT GACCACCACT ATTCCGTAAG AAATAGTGAC 
TCTGAATGTC CCCTGTCCCA CGATGGGTAC TGCCTCCATG ATGGTGTGTG CATGTATATT 
GAAGCATTGG ACAAGTATGC ATGCAACTGT GTTGTTGGCT ACATCGGGGA GCGATGTCAG 
TACCGAGACC TGAAGTGGTG GGAACTGCGC CACGCTGGCC ACGGGCAGCA GCAGAAGGTC 
ATCGTGGTGG CTGTCTGCGT GGTGGTGCTT GTCATGCTGC TCCTCCTGAG CCTGTGGGGG 
GCCCACTACT ACAGGACTCA GAAGCTGCTA TCGAAAAACC CAAAGAATCC TTATGAGGAG 
TCGAGCAGAG ATGTGAGGAG TCGCAGGCCT GCTGACACTG AGGATGGGAT GTCCTCTTGC 
CCTCAACCTT GGTTTGTGGT TATAAAAGAA CACCAAGACC TCAAGAATGG GGGTCAACCA 
GTGGCTGGTG AGGATGGCCA GGCAGCAGAT GGTCAGTTTT TATCCCTGGC TCCTGGGTCA 
ATGCAACCAA CTTCATGGAG GCAGGAGCCC CAGTTATGTG GAATGGGCAC AGAGCAAGGC 
TGCTGGATTC CAGTATCCAG TGATAAGGGC TCCTGTCCCC AGGTAATGGA GCGAAGCTTT 
CATATGCCCT CCTATGGGAC ACAGACCCTT GAAGGGGGTG TCGAGAAGCC CCATTCTCTC 
CTATCAGCTA ACCCATTATG GCAACAAAGG GCCCTGGACC CACCACACCA AATGGAGCTG 
ACTCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016863338.1
CDS	454..3882
Translation	MLLTLIILLPVVSKFSFVSLSAPQHWSCPEGTLAGNGNSTCVVWSLSSHPGPAPFLIFSHGNSIFRIDTEGTNYEQLVVDAGVSVIMDFHYNEKRIYWVDLERQLLQRVFLNGSRQERVCNIEKNVSGMAINWINEEVIWSNQQEGIITVTDMKGNNSHILLSALKYPANVAVDPVERFIFWSSEVAGSLYRADLDGVGVKALLETSEKITAVSLDVLDKRLFWIQYNREGSNSLICSCDYDGGSVHISKHPTQHNLFAMSLFGDRIFYSTWKMKTIWIANKHTGKDMVRINLHSSFVPLGELKVVHPLAQPKAEDDTWEPEQKLCKLRKGNCSSTVCGQDLQSHLCMCAEGYALSRDRKYCEDVNECAFWNHGCTLGCKNTPGSYYCTCPVGFVLLPDGKRCHQLVSCPRNVSECSHDCVLTSEGPLCFCPEGSVLERDGKTCSGCSSPDNGGCSQLCVPLSPVSWECDCFPGYDLQLDEKSCAASGPQPFLLFANSQDIRHMHFDGTDYGTLLSQQMGMVYALDHDPVENKIYFAHTALKWIERANMDGSQRERLIEEGVDVPEGLAVDWIGRRFYWTDRGKSLIGRSDLNGKRSKIITKENISQPRGIAVHPMAKRLFWTDTGINPRIESSSLQGLGRLVIASSDLIWPSGITIDFLTDKLYWCDAKQSVIEMANLDGSKRRRLTQNDVGHPFAVAVFEDYVWFSDWAMPSVMRVNKRTGKDRVRLQGSMLKPSSLVVVHPLAKPGADPCLYQNGGCEHICKKRLGTAWCSCREGFMKASDGKTCLALDGHQLLAGGEVDLKNQVTPLDILSKTRVSEDNITESQHMLVAEIMVSDIDECQLGEHSCGENASCTNTEGGYTCMCAGRLSEPGLICPDSTPPPHLREDDHHYSVRNSDSECPLSHDGYCLHDGVCMYIEALDKYACNCVVGYIGERCQYRDLKWWELRHAGHGQQQKVIVVAVCVVVLVMLLLLSLWGAHYYRTQKLLSKNPKNPYEESSRDVRSRRPADTEDGMSSCPQPWFVVIKEHQDLKNGGQPVAGEDGQAADGQFLSLAPGSMQPTSWRQEPQLCGMGTEQGCWIPVSSDKGSCPQVMERSFHMPSYGTQTLEGGVEKPHSLLSANPLWQQRALDPPHQMELTQ

Target ORF information:

RefSeq Version	XM_017007849.1
Organism	Homo sapiens(human)
Definition	Homo sapiens epidermal growth factor (EGF), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017007849.1

ORF Insert Sequence:

ATGCTGCTCA CTCTTATCAT TCTGTTGCCA GTAGTTTCAA AATTTAGTTT TGTTAGTCTC 
TCAGCACCGC AGCACTGGAG CTGTCCTGAA GGTACTCTCG CAGGAAATGG GAATTCTACT 
TGTGTGGTTT GGTCTCTTTC TTCCCACCCA GGTCCTGCAC CCTTCTTAAT TTTCTCCCAT 
GGAAATAGTA TCTTTAGGAT TGACACAGAA GGAACCAATT ATGAGCAATT GGTGGTGGAT 
GCTGGTGTCT CAGTGATCAT GGATTTTCAT TATAATGAGA AAAGAATCTA TTGGGTGGAT 
TTAGAAAGAC AACTTTTGCA AAGAGTTTTT CTGAATGGGT CAAGGCAAGA GAGAGTATGT 
AATATAGAGA AAAATGTTTC TGGAATGGCA ATAAATTGGA TAAATGAAGA AGTTATTTGG 
TCAAATCAAC AGGAAGGAAT CATTACAGTA ACAGATATGA AAGGAAATAA TTCCCACATT 
CTTTTAAGTG CTTTAAAATA TCCTGCAAAT GTAGCAGTTG ATCCAGTAGA AAGGTTTATA 
TTTTGGTCTT CAGAGGTGGC TGGAAGCCTT TATAGAGCAG ATCTCGATGG TGTGGGAGTG 
AAGGCTCTGT TGGAGACATC AGAGAAAATA ACAGCTGTGT CATTGGATGT GCTTGATAAG 
CGGCTGTTTT GGATTCAGTA CAACAGAGAA GGAAGCAATT CTCTTATTTG CTCCTGTGAT 
TATGATGGAG GTTCTGTCCA CATTAGTAAA CATCCAACAC AGCATAATTT GTTTGCAATG 
TCCCTTTTTG GTGACCGTAT CTTCTATTCA ACATGGAAAA TGAAGACAAT TTGGATAGCC 
AACAAACACA CTGGAAAGGA CATGGTTAGA ATTAACCTCC ATTCATCATT TGTACCACTT 
GGTGAACTGA AAGTAGTGCA TCCACTTGCA CAACCCAAGG CAGAAGATGA CACTTGGGAG 
CCTGAGCAGA AACTTTGCAA ATTGAGGAAA GGAAACTGCA GCAGCACTGT GTGTGGGCAA 
GACCTCCAGT CACACTTGTG CATGTGTGCA GAGGGATACG CCCTAAGTCG AGACCGGAAG 
TACTGTGAAG ATGTTAATGA ATGTGCTTTT TGGAATCATG GCTGTACTCT TGGGTGTAAA 
AACACCCCTG GATCCTATTA CTGCACGTGC CCTGTAGGAT TTGTTCTGCT TCCTGATGGG 
AAACGATGTC ATCAACTTGT TTCCTGTCCA CGCAATGTGT CTGAATGCAG CCATGACTGT 
GTTCTGACAT CAGAAGGTCC CTTATGTTTC TGTCCTGAAG GCTCAGTGCT TGAGAGAGAT 
GGGAAAACAT GTAGCGGTTG TTCCTCACCC GATAATGGTG GATGTAGCCA GCTCTGCGTT 
CCTCTTAGCC CAGTATCCTG GGAATGTGAT TGCTTTCCTG GGTATGACCT ACAACTGGAT 
GAAAAAAGCT GTGCAGCTTC AGGACCACAA CCATTTTTGC TGTTTGCCAA TTCTCAAGAT 
ATTCGACACA TGCATTTTGA TGGAACAGAC TATGGAACTC TGCTCAGCCA GCAGATGGGA 
ATGGTTTATG CCCTAGATCA TGACCCTGTG GAAAATAAGA TATACTTTGC CCATACAGCC 
CTGAAGTGGA TAGAGAGAGC TAATATGGAT GGTTCCCAGC GAGAAAGGCT TATTGAGGAA 
GGAGTAGATG TGCCAGAAGG TCTTGCTGTG GACTGGATTG GCCGTAGATT CTATTGGACA 
GACAGAGGGA AATCTCTGAT TGGAAGGAGT GATTTAAATG GGAAACGTTC CAAAATAATC 
ACTAAGGAGA ACATCTCTCA ACCACGAGGA ATTGCTGTTC ATCCAATGGC CAAGAGATTA 
TTCTGGACTG ATACAGGGAT TAATCCACGA ATTGAAAGTT CTTCCCTCCA AGGCCTTGGC 
CGTCTGGTTA TAGCCAGCTC TGATCTAATC TGGCCCAGTG GAATAACGAT TGACTTCTTA 
ACTGACAAGT TGTACTGGTG CGATGCCAAG CAGTCTGTGA TTGAAATGGC CAATCTGGAT 
GGTTCAAAAC GCCGAAGACT TACCCAGAAT GATGTAGGTC ACCCATTTGC TGTAGCAGTG 
TTTGAGGATT ATGTGTGGTT CTCAGATTGG GCTATGCCAT CAGTAATGAG AGTAAACAAG 
AGGACTGGCA AAGATAGAGT ACGTCTCCAA GGCAGCATGC TGAAGCCCTC ATCACTGGTT 
GTGGTTCATC CATTGGCAAA ACCAGGAGCA GATCCCTGCT TATATCAAAA CGGAGGCTGT 
GAACATATTT GCAAAAAGAG GCTTGGAACT GCTTGGTGTT CGTGTCGTGA AGGTTTTATG 
AAAGCCTCAG ATGGGAAAAC GTGTCTGGCT CTGGATGGTC ATCAGCTGTT GGCAGGTGGT 
GAAGTTGATC TAAAGAACCA AGTAACACCA TTGGACATCT TGTCCAAGAC TAGAGTGTCA 
GAAGATAACA TTACAGAATC TCAACACATG CTAGTGGCTG AAATCATGGT GTCAGATATT 
GATGAGTGCC AACTGGGGGA GCACAGCTGT GGAGAGAATG CCAGCTGCAC AAATACAGAG 
GGAGGCTATA CCTGCATGTG TGCTGGACGC CTGTCTGAAC CAGGACTGAT TTGCCCTGAC 
TCTACTCCAC CCCCTCACCT CAGGGAAGAT GACCACCACT ATTCCGTAAG AAATAGTGAC 
TCTGAATGTC CCCTGTCCCA CGATGGGTAC TGCCTCCATG ATGGTGTGTG CATGTATATT 
GAAGCATTGG ACAAGTATGC ATGCAACTGT GTTGTTGGCT ACATCGGGGA GCGATGTCAG 
TACCGAGACC TGAAGTGGTG GGAACTGCGC CACGCTGGCC ACGGGCAGCA GCAGAAGGTC 
ATCGTGGTGG CTGTCTGCGT GGTGGTGCTT GTCATGCTGC TCCTCCTGAG CCTGTGGGGG 
GCCCACTACT ACAGGACTCA GAAGCTGCTA TCGAAAAACC CAAAGAATCC TTATGAGGAG 
TCGAGCAGAG ATGTGAGGAG TCGCAGGCCT GCTGACACTG AGGATGGGAT GTCCTCTTGC 
CCTCAACCTT GGTTTGTGGT TATAAAAGAA CACCAAGACC TCAAGAATGG GGGTCAACCA 
GTGGCTGGTG AGGATGGCCA GGCAGCAGAT GGTCAGTTTT TATCCCTGGC TCCTGGGTCA 
ATGCAACCAA CTTCATGGAG GCAGGAGCCC CAGTTATGTG GAATGGGCAC AGAGCAAGGC 
TGCTGGATTC CAGTATCCAG TGATAAGGGC TCCTGTCCCC AGGTAATGGA GCGAAGCTTT 
CATATGCCCT CCTATGGGAC ACAGACCCTT GAAGGGGGTG TCGAGAAGCC CCATTCTCTC 
CTATCAGCTA ACCCATTATG GCAACAAAGG GCCCTGGACC CACCACACCA AATGGAGCTG 
ACTCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EGF ( XM_017007850.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016863339.1 Homo sapiens epidermal growth factor (EGF), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OHu94955
Clone ID Related Accession (Same CDS sequence)	XM_017007850.1
Accession Version	XM_017007850.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3426bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	pro-epidermal growth factor isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCTCA CTCTTATCAT TCTGTTGCCA GTAGTTTCAA AATTTAGTTT TGTTAGTCTC 
TCAGCACCGC AGCACTGGAG CTGTCCTGAA GGTACTCTCG CAGGAAATGG GAATTCTACT 
TGTGTGGTTT GGTCTCTTTC TTCCCACCCA GGTCCTGCAC CCTTCTTAAT TTTCTCCCAT 
GGAAATAGTA TCTTTAGGAT TGACACAGAA GGAACCAATT ATGAGCAATT GGTGGTGGAT 
GCTGGTGTCT CAGTGATCAT GGATTTTCAT TATAATGAGA AAAGAATCTA TTGGGTGGAT 
TTAGAAAGAC AACTTTTGCA AAGAGTTTTT CTGAATGGGT CAAGGCAAGA GAGAGTATGT 
AATATAGAGA AAAATGTTTC TGGAATGGCA ATAAATTGGA TAAATGAAGA AGTTATTTGG 
TCAAATCAAC AGGAAGGAAT CATTACAGTA ACAGATATGA AAGGAAATAA TTCCCACATT 
CTTTTAAGTG CTTTAAAATA TCCTGCAAAT GTAGCAGTTG ATCCAGTAGA AAGGTTTATA 
TTTTGGTCTT CAGAGGTGGC TGGAAGCCTT TATAGAGCAG ATCTCGATGG TGTGGGAGTG 
AAGGCTCTGT TGGAGACATC AGAGAAAATA ACAGCTGTGT CATTGGATGT GCTTGATAAG 
CGGCTGTTTT GGATTCAGTA CAACAGAGAA GGAAGCAATT CTCTTATTTG CTCCTGTGAT 
TATGATGGAG GTTCTGTCCA CATTAGTAAA CATCCAACAC AGCATAATTT GTTTGCAATG 
TCCCTTTTTG GTGACCGTAT CTTCTATTCA ACATGGAAAA TGAAGACAAT TTGGATAGCC 
AACAAACACA CTGGAAAGGA CATGGTTAGA ATTAACCTCC ATTCATCATT TGTACCACTT 
GGTGAACTGA AAGTAGTGCA TCCACTTGCA CAACCCAAGG CAGAAGATGA CACTTGGGAG 
CCTGAGCAGA AACTTTGCAA ATTGAGGAAA GGAAACTGCA GCAGCACTGT GTGTGGGCAA 
GACCTCCAGT CACACTTGTG CATGTGTGCA GAGGGATACG CCCTAAGTCG AGACCGGAAG 
TACTGTGAAG ATGTTAATGA ATGTGCTTTT TGGAATCATG GCTGTACTCT TGGGTGTAAA 
AACACCCCTG GATCCTATTA CTGCACGTGC CCTGTAGGAT TTGTTCTGCT TCCTGATGGG 
AAACGATGTC ATCAACTTGT TTCCTGTCCA CGCAATGTGT CTGAATGCAG CCATGACTGT 
GTTCTGACAT CAGAAGGTCC CTTATGTTTC TGTCCTGAAG GCTCAGTGCT TGAGAGAGAT 
GGGAAAACAT GTAGCGGTTG TTCCTCACCC GATAATGGTG GATGTAGCCA GCTCTGCGTT 
CCTCTTAGCC CAGTATCCTG GGAATGTGAT TGCTTTCCTG GGTATGACCT ACAACTGGAT 
GAAAAAAGCT GTGCAGCTTC AGGACCACAA CCATTTTTGC TGTTTGCCAA TTCTCAAGAT 
ATTCGACACA TGCATTTTGA TGGAACAGAC TATGGAACTC TGCTCAGCCA GCAGATGGGA 
ATGGTTTATG CCCTAGATCA TGACCCTGTG GAAAATAAGA TATACTTTGC CCATACAGCC 
CTGAAGTGGA TAGAGAGAGC TAATATGGAT GGTTCCCAGC GAGAAAGGCT TATTGAGGAA 
GGAGTAGATG TGCCAGAAGG TCTTGCTGTG GACTGGATTG GCCGTAGATT CTATTGGACA 
GACAGAGGGA AATCTCTGAT TGGAAGGAGT GATTTAAATG GGAAACGTTC CAAAATAATC 
ACTAAGGAGA ACATCTCTCA ACCACGAGGA ATTGCTGTTC ATCCAATGGC CAAGAGATTA 
TTCTGGACTG ATACAGGGAT TAATCCACGA ATTGAAAGTT CTTCCCTCCA AGGCCTTGGC 
CGTCTGGTTA TAGCCAGCTC TGATCTAATC TGGCCCAGTG GAATAACGAT TGACTTCTTA 
ACTGACAAGT TGTACTGGTG CGATGCCAAG CAGTCTGTGA TTGAAATGGC CAATCTGGAT 
GGTTCAAAAC GCCGAAGACT TACCCAGAAT GATGTAGGTC ACCCATTTGC TGTAGCAGTG 
TTTGAGGATT ATGTGTGGTT CTCAGATTGG GCTATGCCAT CAGTAATGAG AGTAAACAAG 
AGGACTGGCA AAGATAGAGT ACGTCTCCAA GGCAGCATGC TGAAGCCCTC ATCACTGGTT 
GTGGTTCATC CATTGGCAAA ACCAGGAGCA GATCCCTGCT TATATCAAAA CGGAGGCTGT 
GAACATATTT GCAAAAAGAG GCTTGGAACT GCTTGGTGTT CGTGTCGTGA AGGTTTTATG 
AAAGCCTCAG ATGGGAAAAC GTGTCTGGCT CTGGATGGTC ATCAGCTGTT GGCAGGTGGT 
GAAGTTGATC TAAAGAACCA AGTAACACCA TTGGACATCT TGTCCAAGAC TAGAGTGTCA 
GAAGATAACA TTACAGAATC TCAACACATG CTAGTGGCTG AAATCATGGT GTCAGATCAA 
GATGACTGTG CTCCTGTGGG ATGCAGCATG TATGCTCGGT GTATTTCAGA GGGAGAGGAT 
GCCACATGTC AGTGTTTGAA AGGATTTGCT GGGGATGGAA AACTATGTTC TGATATAGAT 
GAATGTGAGA TGGGTGTCCC AGTGTGCCCC CCTGCCTCCT CCAAGTGCAT CAACACCGAA 
GGTGGTTATG TCTGCCGGTG CTCAGAAGGC TACCAAGGAG ATGGGATTCA CTGTCTTGAT 
ATTGATGAGT GCCAACTGGG GGAGCACAGC TGTGGAGAGA ATGCCAGCTG CACAAATACA 
GAGGGAGGCT ATACCTGCAT GTGTGCTGGA CGCCTGTCTG AACCAGGACT GATTTGCCCT 
GACTCTACTC CACCCCCTCA CCTCAGGGAA GATGACCACC ACTATTCCGT AAGAAATAGT 
GACTCTGAAT GTCCCCTGTC CCACGATGGG TACTGCCTCC ATGATGGTGT GTGCATGTAT 
ATTGAAGCAT TGGACAAGTA TGCATGCAAC TGTGTTGTTG GCTACATCGG GGAGCGATGT 
CAGTACCGAG ACCTGAAGTG GTGGGAACTG CGCCACGCTG GCCACGGGCA GCAGCAGAAG 
GTCATCGTGG TGGCTGTCTG CGTGGTGGTG CTTGTCATGC TGCTCCTCCT GAGCCTGTGG 
GGGGCCCACT ACTACAGGAC TCAGAAGCTG CTATCGAAAA ACCCAAAGAA TCCTTATGAG 
GAGTCGAGCA GAGATGTGAG GAGTCGCAGG CCTGCTGACA CTGAGGATGG GATGTCCTCT 
TGCCCTCAAC CTTGGGGTCA ATGCAACCAA CTTCATGGAG GCAGGAGCCC CAGTTATGTG 
GAATGGGCAC AGAGCAAGGC TGCTGGATTC CAGTATCCAG TGATAAGGGC TCCTGTCCCC 
AGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016863339.1
CDS	454..3879
Translation	MLLTLIILLPVVSKFSFVSLSAPQHWSCPEGTLAGNGNSTCVVWSLSSHPGPAPFLIFSHGNSIFRIDTEGTNYEQLVVDAGVSVIMDFHYNEKRIYWVDLERQLLQRVFLNGSRQERVCNIEKNVSGMAINWINEEVIWSNQQEGIITVTDMKGNNSHILLSALKYPANVAVDPVERFIFWSSEVAGSLYRADLDGVGVKALLETSEKITAVSLDVLDKRLFWIQYNREGSNSLICSCDYDGGSVHISKHPTQHNLFAMSLFGDRIFYSTWKMKTIWIANKHTGKDMVRINLHSSFVPLGELKVVHPLAQPKAEDDTWEPEQKLCKLRKGNCSSTVCGQDLQSHLCMCAEGYALSRDRKYCEDVNECAFWNHGCTLGCKNTPGSYYCTCPVGFVLLPDGKRCHQLVSCPRNVSECSHDCVLTSEGPLCFCPEGSVLERDGKTCSGCSSPDNGGCSQLCVPLSPVSWECDCFPGYDLQLDEKSCAASGPQPFLLFANSQDIRHMHFDGTDYGTLLSQQMGMVYALDHDPVENKIYFAHTALKWIERANMDGSQRERLIEEGVDVPEGLAVDWIGRRFYWTDRGKSLIGRSDLNGKRSKIITKENISQPRGIAVHPMAKRLFWTDTGINPRIESSSLQGLGRLVIASSDLIWPSGITIDFLTDKLYWCDAKQSVIEMANLDGSKRRRLTQNDVGHPFAVAVFEDYVWFSDWAMPSVMRVNKRTGKDRVRLQGSMLKPSSLVVVHPLAKPGADPCLYQNGGCEHICKKRLGTAWCSCREGFMKASDGKTCLALDGHQLLAGGEVDLKNQVTPLDILSKTRVSEDNITESQHMLVAEIMVSDQDDCAPVGCSMYARCISEGEDATCQCLKGFAGDGKLCSDIDECEMGVPVCPPASSKCINTEGGYVCRCSEGYQGDGIHCLDIDECQLGEHSCGENASCTNTEGGYTCMCAGRLSEPGLICPDSTPPPHLREDDHHYSVRNSDSECPLSHDGYCLHDGVCMYIEALDKYACNCVVGYIGERCQYRDLKWWELRHAGHGQQQKVIVVAVCVVVLVMLLLLSLWGAHYYRTQKLLSKNPKNPYEESSRDVRSRRPADTEDGMSSCPQPWGQCNQLHGGRSPSYVEWAQSKAAGFQYPVIRAPVPR

Target ORF information:

RefSeq Version	XM_017007850.1
Organism	Homo sapiens(human)
Definition	Homo sapiens epidermal growth factor (EGF), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017007850.1

ORF Insert Sequence:

ATGCTGCTCA CTCTTATCAT TCTGTTGCCA GTAGTTTCAA AATTTAGTTT TGTTAGTCTC 
TCAGCACCGC AGCACTGGAG CTGTCCTGAA GGTACTCTCG CAGGAAATGG GAATTCTACT 
TGTGTGGTTT GGTCTCTTTC TTCCCACCCA GGTCCTGCAC CCTTCTTAAT TTTCTCCCAT 
GGAAATAGTA TCTTTAGGAT TGACACAGAA GGAACCAATT ATGAGCAATT GGTGGTGGAT 
GCTGGTGTCT CAGTGATCAT GGATTTTCAT TATAATGAGA AAAGAATCTA TTGGGTGGAT 
TTAGAAAGAC AACTTTTGCA AAGAGTTTTT CTGAATGGGT CAAGGCAAGA GAGAGTATGT 
AATATAGAGA AAAATGTTTC TGGAATGGCA ATAAATTGGA TAAATGAAGA AGTTATTTGG 
TCAAATCAAC AGGAAGGAAT CATTACAGTA ACAGATATGA AAGGAAATAA TTCCCACATT 
CTTTTAAGTG CTTTAAAATA TCCTGCAAAT GTAGCAGTTG ATCCAGTAGA AAGGTTTATA 
TTTTGGTCTT CAGAGGTGGC TGGAAGCCTT TATAGAGCAG ATCTCGATGG TGTGGGAGTG 
AAGGCTCTGT TGGAGACATC AGAGAAAATA ACAGCTGTGT CATTGGATGT GCTTGATAAG 
CGGCTGTTTT GGATTCAGTA CAACAGAGAA GGAAGCAATT CTCTTATTTG CTCCTGTGAT 
TATGATGGAG GTTCTGTCCA CATTAGTAAA CATCCAACAC AGCATAATTT GTTTGCAATG 
TCCCTTTTTG GTGACCGTAT CTTCTATTCA ACATGGAAAA TGAAGACAAT TTGGATAGCC 
AACAAACACA CTGGAAAGGA CATGGTTAGA ATTAACCTCC ATTCATCATT TGTACCACTT 
GGTGAACTGA AAGTAGTGCA TCCACTTGCA CAACCCAAGG CAGAAGATGA CACTTGGGAG 
CCTGAGCAGA AACTTTGCAA ATTGAGGAAA GGAAACTGCA GCAGCACTGT GTGTGGGCAA 
GACCTCCAGT CACACTTGTG CATGTGTGCA GAGGGATACG CCCTAAGTCG AGACCGGAAG 
TACTGTGAAG ATGTTAATGA ATGTGCTTTT TGGAATCATG GCTGTACTCT TGGGTGTAAA 
AACACCCCTG GATCCTATTA CTGCACGTGC CCTGTAGGAT TTGTTCTGCT TCCTGATGGG 
AAACGATGTC ATCAACTTGT TTCCTGTCCA CGCAATGTGT CTGAATGCAG CCATGACTGT 
GTTCTGACAT CAGAAGGTCC CTTATGTTTC TGTCCTGAAG GCTCAGTGCT TGAGAGAGAT 
GGGAAAACAT GTAGCGGTTG TTCCTCACCC GATAATGGTG GATGTAGCCA GCTCTGCGTT 
CCTCTTAGCC CAGTATCCTG GGAATGTGAT TGCTTTCCTG GGTATGACCT ACAACTGGAT 
GAAAAAAGCT GTGCAGCTTC AGGACCACAA CCATTTTTGC TGTTTGCCAA TTCTCAAGAT 
ATTCGACACA TGCATTTTGA TGGAACAGAC TATGGAACTC TGCTCAGCCA GCAGATGGGA 
ATGGTTTATG CCCTAGATCA TGACCCTGTG GAAAATAAGA TATACTTTGC CCATACAGCC 
CTGAAGTGGA TAGAGAGAGC TAATATGGAT GGTTCCCAGC GAGAAAGGCT TATTGAGGAA 
GGAGTAGATG TGCCAGAAGG TCTTGCTGTG GACTGGATTG GCCGTAGATT CTATTGGACA 
GACAGAGGGA AATCTCTGAT TGGAAGGAGT GATTTAAATG GGAAACGTTC CAAAATAATC 
ACTAAGGAGA ACATCTCTCA ACCACGAGGA ATTGCTGTTC ATCCAATGGC CAAGAGATTA 
TTCTGGACTG ATACAGGGAT TAATCCACGA ATTGAAAGTT CTTCCCTCCA AGGCCTTGGC 
CGTCTGGTTA TAGCCAGCTC TGATCTAATC TGGCCCAGTG GAATAACGAT TGACTTCTTA 
ACTGACAAGT TGTACTGGTG CGATGCCAAG CAGTCTGTGA TTGAAATGGC CAATCTGGAT 
GGTTCAAAAC GCCGAAGACT TACCCAGAAT GATGTAGGTC ACCCATTTGC TGTAGCAGTG 
TTTGAGGATT ATGTGTGGTT CTCAGATTGG GCTATGCCAT CAGTAATGAG AGTAAACAAG 
AGGACTGGCA AAGATAGAGT ACGTCTCCAA GGCAGCATGC TGAAGCCCTC ATCACTGGTT 
GTGGTTCATC CATTGGCAAA ACCAGGAGCA GATCCCTGCT TATATCAAAA CGGAGGCTGT 
GAACATATTT GCAAAAAGAG GCTTGGAACT GCTTGGTGTT CGTGTCGTGA AGGTTTTATG 
AAAGCCTCAG ATGGGAAAAC GTGTCTGGCT CTGGATGGTC ATCAGCTGTT GGCAGGTGGT 
GAAGTTGATC TAAAGAACCA AGTAACACCA TTGGACATCT TGTCCAAGAC TAGAGTGTCA 
GAAGATAACA TTACAGAATC TCAACACATG CTAGTGGCTG AAATCATGGT GTCAGATCAA 
GATGACTGTG CTCCTGTGGG ATGCAGCATG TATGCTCGGT GTATTTCAGA GGGAGAGGAT 
GCCACATGTC AGTGTTTGAA AGGATTTGCT GGGGATGGAA AACTATGTTC TGATATAGAT 
GAATGTGAGA TGGGTGTCCC AGTGTGCCCC CCTGCCTCCT CCAAGTGCAT CAACACCGAA 
GGTGGTTATG TCTGCCGGTG CTCAGAAGGC TACCAAGGAG ATGGGATTCA CTGTCTTGAT 
ATTGATGAGT GCCAACTGGG GGAGCACAGC TGTGGAGAGA ATGCCAGCTG CACAAATACA 
GAGGGAGGCT ATACCTGCAT GTGTGCTGGA CGCCTGTCTG AACCAGGACT GATTTGCCCT 
GACTCTACTC CACCCCCTCA CCTCAGGGAA GATGACCACC ACTATTCCGT AAGAAATAGT 
GACTCTGAAT GTCCCCTGTC CCACGATGGG TACTGCCTCC ATGATGGTGT GTGCATGTAT 
ATTGAAGCAT TGGACAAGTA TGCATGCAAC TGTGTTGTTG GCTACATCGG GGAGCGATGT 
CAGTACCGAG ACCTGAAGTG GTGGGAACTG CGCCACGCTG GCCACGGGCA GCAGCAGAAG 
GTCATCGTGG TGGCTGTCTG CGTGGTGGTG CTTGTCATGC TGCTCCTCCT GAGCCTGTGG 
GGGGCCCACT ACTACAGGAC TCAGAAGCTG CTATCGAAAA ACCCAAAGAA TCCTTATGAG 
GAGTCGAGCA GAGATGTGAG GAGTCGCAGG CCTGCTGACA CTGAGGATGG GATGTCCTCT 
TGCCCTCAAC CTTGGGGTCA ATGCAACCAA CTTCATGGAG GCAGGAGCCC CAGTTATGTG 
GAATGGGCAC AGAGCAAGGC TGCTGGATTC CAGTATCCAG TGATAAGGGC TCCTGTCCCC 
AGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EGF ( XM_017007851.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016863340.1 Homo sapiens epidermal growth factor (EGF), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OHu94956
Clone ID Related Accession (Same CDS sequence)	XM_017007851.1
Accession Version	XM_017007851.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3183bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	pro-epidermal growth factor isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCTCA CTCTTATCAT TCTGTTGCCA GTAGTTTCAA AATTTAGTTT TGTTAGTCTC 
TCAGCACCGC AGCACTGGAG CTGTCCTGAA GGTACTCTCG CAGGAAATGG GAATTCTACT 
TGTGTGGTTT GGTCTCTTTC TTCCCACCCA GGTCCTGCAC CCTTCTTAAT TTTCTCCCAT 
GGAAATAGTA TCTTTAGGAT TGACACAGAA GGAACCAATT ATGAGCAATT GGTGGTGGAT 
GCTGGTGTCT CAGTGATCAT GGATTTTCAT TATAATGAGA AAAGAATCTA TTGGGTGGAT 
TTAGAAAGAC AACTTTTGCA AAGAGTTTTT CTGAATGGGT CAAGGCAAGA GAGAGTATGT 
AATATAGAGA AAAATGTTTC TGGAATGGCA ATAAATTGGA TAAATGAAGA AGTTATTTGG 
TCAAATCAAC AGGAAGGAAT CATTACAGTA ACAGATATGA AAGGAAATAA TTCCCACATT 
CTTTTAAGTG CTTTAAAATA TCCTGCAAAT GTAGCAGTTG ATCCAGTAGA AAGGTTTATA 
TTTTGGTCTT CAGAGGTGGC TGGAAGCCTT TATAGAGCAG ATCTCGATGG TGTGGGAGTG 
AAGGCTCTGT TGGAGACATC AGAGAAAATA ACAGCTGTGT CATTGGATGT GCTTGATAAG 
CGGCTGTTTT GGATTCAGTA CAACAGAGAA GGAAGCAATT CTCTTATTTG CTCCTGTGAT 
TATGATGGAG GTTCTGTCCA CATTAGTAAA CATCCAACAC AGCATAATTT GTTTGCAATG 
TCCCTTTTTG GTGACCGTAT CTTCTATTCA ACATGGAAAA TGAAGACAAT TTGGATAGCC 
AACAAACACA CTGGAAAGGA CATGGTTAGA ATTAACCTCC ATTCATCATT TGTACCACTT 
GGTGAACTGA AAGTAGTGCA TCCACTTGCA CAACCCAAGG CAGAAGATGA CACTTGGGAG 
CCTGAGCAGA AACTTTGCAA ATTGAGGAAA GGAAACTGCA GCAGCACTGT GTGTGGGCAA 
GACCTCCAGT CACACTTGTG CATGTGTGCA GAGGGATACG CCCTAAGTCG AGACCGGAAG 
TACTGTGAAG ATGTTAATGA ATGTGCTTTT TGGAATCATG GCTGTACTCT TGGGTGTAAA 
AACACCCCTG GATCCTATTA CTGCACGTGC CCTGTAGGAT TTGTTCTGCT TCCTGATGGG 
AAACGATGTC ATCAACTTGT TTCCTGTCCA CGCAATGTGT CTGAATGCAG CCATGACTGT 
GTTCTGACAT CAGAAGGTCC CTTATGTTTC TGTCCTGAAG GCTCAGTGCT TGAGAGAGAT 
GGGAAAACAT GTAGCGGTTG TTCCTCACCC GATAATGGTG GATGTAGCCA GCTCTGCGTT 
CCTCTTAGCC CAGTATCCTG GGAATGTGAT TGCTTTCCTG GGTATGACCT ACAACTGGAT 
GAAAAAAGCT GTGCAGCTTC AGGACCACAA CCATTTTTGC TGTTTGCCAA TTCTCAAGAT 
ATTCGACACA TGCATTTTGA TGGAACAGAC TATGGAACTC TGCTCAGCCA GCAGATGGGA 
ATGGTTTATG CCCTAGATCA TGACCCTGTG GAAAATAAGA TATACTTTGC CCATACAGCC 
CTGAAGTGGA TAGAGAGAGC TAATATGGAT GGTTCCCAGC GAGAAAGGCT TATTGAGGAA 
GGAGTAGATG TGCCAGAAGG TCTTGCTGTG GACTGGATTG GCCGTAGATT CTATTGGACA 
GACAGAGGGA AATCTCTGAT TGGAAGGAGT GATTTAAATG GGAAACGTTC CAAAATAATC 
ACTAAGGAGA ACATCTCTCA ACCACGAGGA ATTGCTGTTC ATCCAATGGC CAAGAGATTA 
TTCTGGACTG ATACAGGGAT TAATCCACGA ATTGAAAGTT CTTCCCTCCA AGGCCTTGGC 
CGTCTGGTTA TAGCCAGCTC TGATCTAATC TGGCCCAGTG GAATAACGAT TGACTTCTTA 
ACTGACAAGT TGTACTGGTG CGATGCCAAG CAGTCTGTGA TTGAAATGGC CAATCTGGAT 
GGTTCAAAAC GCCGAAGACT TACCCAGAAT GATGTAGGTC ACCCATTTGC TGTAGCAGTG 
TTTGAGGATT ATGTGTGGTT CTCAGATTGG GCTATGCCAT CAGTAATGAG AGTAAACAAG 
AGGACTGGCA AAGATAGAGT ACGTCTCCAA GGCAGCATGC TGAAGCCCTC ATCACTGGTT 
GTGGTTCATC CATTGGCAAA ACCAGGAGCA GATCCCTGCT TATATCAAAA CGGAGGCTGT 
GAACATATTT GCAAAAAGAG GCTTGGAACT GCTTGGTGTT CGTGTCGTGA AGGTTTTATG 
AAAGCCTCAG ATGGGAAAAC GTGTCTGGCT CTGGATGGTC ATCAGCTGTT GGCAGGTGGT 
GAAGTTGATC TAAAGAACCA AGTAACACCA TTGGACATCT TGTCCAAGAC TAGAGTGTCA 
GAAGATAACA TTACAGAATC TCAACACATG CTAGTGGCTG AAATCATGGT GTCAGATATT 
GATGAGTGCC AACTGGGGGA GCACAGCTGT GGAGAGAATG CCAGCTGCAC AAATACAGAG 
GGAGGCTATA CCTGCATGTG TGCTGGACGC CTGTCTGAAC CAGGACTGAT TTGCCCTGAC 
TCTACTCCAC CCCCTCACCT CAGGGAAGAT GACCACCACT ATTCCGTAAG AAATAGTGAC 
TCTGAATGTC CCCTGTCCCA CGATGGGTAC TGCCTCCATG ATGGTGTGTG CATGTATATT 
GAAGCATTGG ACAAGTATGC ATGCAACTGT GTTGTTGGCT ACATCGGGGA GCGATGTCAG 
TACCGAGACC TGAAGTGGTG GGAACTGCGC CACGCTGGCC ACGGGCAGCA GCAGAAGGTC 
ATCGTGGTGG CTGTCTGCGT GGTGGTGCTT GTCATGCTGC TCCTCCTGAG CCTGTGGGGG 
GCCCACTACT ACAGGACTCA GAAGCTGCTA TCGAAAAACC CAAAGAATCC TTATGAGGAG 
TCGAGCAGAG ATGTGAGGAG TCGCAGGCCT GCTGACACTG AGGATGGGAT GTCCTCTTGC 
CCTCAACCTT GGGGTCAATG CAACCAACTT CATGGAGGCA GGAGCCCCAG TTATGTGGAA 
TGGGCACAGA GCAAGGCTGC TGGATTCCAG TATCCAGTGA TAAGGGCTCC TGTCCCCAGG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016863340.1
CDS	454..3636
Translation	MLLTLIILLPVVSKFSFVSLSAPQHWSCPEGTLAGNGNSTCVVWSLSSHPGPAPFLIFSHGNSIFRIDTEGTNYEQLVVDAGVSVIMDFHYNEKRIYWVDLERQLLQRVFLNGSRQERVCNIEKNVSGMAINWINEEVIWSNQQEGIITVTDMKGNNSHILLSALKYPANVAVDPVERFIFWSSEVAGSLYRADLDGVGVKALLETSEKITAVSLDVLDKRLFWIQYNREGSNSLICSCDYDGGSVHISKHPTQHNLFAMSLFGDRIFYSTWKMKTIWIANKHTGKDMVRINLHSSFVPLGELKVVHPLAQPKAEDDTWEPEQKLCKLRKGNCSSTVCGQDLQSHLCMCAEGYALSRDRKYCEDVNECAFWNHGCTLGCKNTPGSYYCTCPVGFVLLPDGKRCHQLVSCPRNVSECSHDCVLTSEGPLCFCPEGSVLERDGKTCSGCSSPDNGGCSQLCVPLSPVSWECDCFPGYDLQLDEKSCAASGPQPFLLFANSQDIRHMHFDGTDYGTLLSQQMGMVYALDHDPVENKIYFAHTALKWIERANMDGSQRERLIEEGVDVPEGLAVDWIGRRFYWTDRGKSLIGRSDLNGKRSKIITKENISQPRGIAVHPMAKRLFWTDTGINPRIESSSLQGLGRLVIASSDLIWPSGITIDFLTDKLYWCDAKQSVIEMANLDGSKRRRLTQNDVGHPFAVAVFEDYVWFSDWAMPSVMRVNKRTGKDRVRLQGSMLKPSSLVVVHPLAKPGADPCLYQNGGCEHICKKRLGTAWCSCREGFMKASDGKTCLALDGHQLLAGGEVDLKNQVTPLDILSKTRVSEDNITESQHMLVAEIMVSDIDECQLGEHSCGENASCTNTEGGYTCMCAGRLSEPGLICPDSTPPPHLREDDHHYSVRNSDSECPLSHDGYCLHDGVCMYIEALDKYACNCVVGYIGERCQYRDLKWWELRHAGHGQQQKVIVVAVCVVVLVMLLLLSLWGAHYYRTQKLLSKNPKNPYEESSRDVRSRRPADTEDGMSSCPQPWGQCNQLHGGRSPSYVEWAQSKAAGFQYPVIRAPVPR

Target ORF information:

RefSeq Version	XM_017007851.1
Organism	Homo sapiens(human)
Definition	Homo sapiens epidermal growth factor (EGF), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017007851.1

ORF Insert Sequence:

ATGCTGCTCA CTCTTATCAT TCTGTTGCCA GTAGTTTCAA AATTTAGTTT TGTTAGTCTC 
TCAGCACCGC AGCACTGGAG CTGTCCTGAA GGTACTCTCG CAGGAAATGG GAATTCTACT 
TGTGTGGTTT GGTCTCTTTC TTCCCACCCA GGTCCTGCAC CCTTCTTAAT TTTCTCCCAT 
GGAAATAGTA TCTTTAGGAT TGACACAGAA GGAACCAATT ATGAGCAATT GGTGGTGGAT 
GCTGGTGTCT CAGTGATCAT GGATTTTCAT TATAATGAGA AAAGAATCTA TTGGGTGGAT 
TTAGAAAGAC AACTTTTGCA AAGAGTTTTT CTGAATGGGT CAAGGCAAGA GAGAGTATGT 
AATATAGAGA AAAATGTTTC TGGAATGGCA ATAAATTGGA TAAATGAAGA AGTTATTTGG 
TCAAATCAAC AGGAAGGAAT CATTACAGTA ACAGATATGA AAGGAAATAA TTCCCACATT 
CTTTTAAGTG CTTTAAAATA TCCTGCAAAT GTAGCAGTTG ATCCAGTAGA AAGGTTTATA 
TTTTGGTCTT CAGAGGTGGC TGGAAGCCTT TATAGAGCAG ATCTCGATGG TGTGGGAGTG 
AAGGCTCTGT TGGAGACATC AGAGAAAATA ACAGCTGTGT CATTGGATGT GCTTGATAAG 
CGGCTGTTTT GGATTCAGTA CAACAGAGAA GGAAGCAATT CTCTTATTTG CTCCTGTGAT 
TATGATGGAG GTTCTGTCCA CATTAGTAAA CATCCAACAC AGCATAATTT GTTTGCAATG 
TCCCTTTTTG GTGACCGTAT CTTCTATTCA ACATGGAAAA TGAAGACAAT TTGGATAGCC 
AACAAACACA CTGGAAAGGA CATGGTTAGA ATTAACCTCC ATTCATCATT TGTACCACTT 
GGTGAACTGA AAGTAGTGCA TCCACTTGCA CAACCCAAGG CAGAAGATGA CACTTGGGAG 
CCTGAGCAGA AACTTTGCAA ATTGAGGAAA GGAAACTGCA GCAGCACTGT GTGTGGGCAA 
GACCTCCAGT CACACTTGTG CATGTGTGCA GAGGGATACG CCCTAAGTCG AGACCGGAAG 
TACTGTGAAG ATGTTAATGA ATGTGCTTTT TGGAATCATG GCTGTACTCT TGGGTGTAAA 
AACACCCCTG GATCCTATTA CTGCACGTGC CCTGTAGGAT TTGTTCTGCT TCCTGATGGG 
AAACGATGTC ATCAACTTGT TTCCTGTCCA CGCAATGTGT CTGAATGCAG CCATGACTGT 
GTTCTGACAT CAGAAGGTCC CTTATGTTTC TGTCCTGAAG GCTCAGTGCT TGAGAGAGAT 
GGGAAAACAT GTAGCGGTTG TTCCTCACCC GATAATGGTG GATGTAGCCA GCTCTGCGTT 
CCTCTTAGCC CAGTATCCTG GGAATGTGAT TGCTTTCCTG GGTATGACCT ACAACTGGAT 
GAAAAAAGCT GTGCAGCTTC AGGACCACAA CCATTTTTGC TGTTTGCCAA TTCTCAAGAT 
ATTCGACACA TGCATTTTGA TGGAACAGAC TATGGAACTC TGCTCAGCCA GCAGATGGGA 
ATGGTTTATG CCCTAGATCA TGACCCTGTG GAAAATAAGA TATACTTTGC CCATACAGCC 
CTGAAGTGGA TAGAGAGAGC TAATATGGAT GGTTCCCAGC GAGAAAGGCT TATTGAGGAA 
GGAGTAGATG TGCCAGAAGG TCTTGCTGTG GACTGGATTG GCCGTAGATT CTATTGGACA 
GACAGAGGGA AATCTCTGAT TGGAAGGAGT GATTTAAATG GGAAACGTTC CAAAATAATC 
ACTAAGGAGA ACATCTCTCA ACCACGAGGA ATTGCTGTTC ATCCAATGGC CAAGAGATTA 
TTCTGGACTG ATACAGGGAT TAATCCACGA ATTGAAAGTT CTTCCCTCCA AGGCCTTGGC 
CGTCTGGTTA TAGCCAGCTC TGATCTAATC TGGCCCAGTG GAATAACGAT TGACTTCTTA 
ACTGACAAGT TGTACTGGTG CGATGCCAAG CAGTCTGTGA TTGAAATGGC CAATCTGGAT 
GGTTCAAAAC GCCGAAGACT TACCCAGAAT GATGTAGGTC ACCCATTTGC TGTAGCAGTG 
TTTGAGGATT ATGTGTGGTT CTCAGATTGG GCTATGCCAT CAGTAATGAG AGTAAACAAG 
AGGACTGGCA AAGATAGAGT ACGTCTCCAA GGCAGCATGC TGAAGCCCTC ATCACTGGTT 
GTGGTTCATC CATTGGCAAA ACCAGGAGCA GATCCCTGCT TATATCAAAA CGGAGGCTGT 
GAACATATTT GCAAAAAGAG GCTTGGAACT GCTTGGTGTT CGTGTCGTGA AGGTTTTATG 
AAAGCCTCAG ATGGGAAAAC GTGTCTGGCT CTGGATGGTC ATCAGCTGTT GGCAGGTGGT 
GAAGTTGATC TAAAGAACCA AGTAACACCA TTGGACATCT TGTCCAAGAC TAGAGTGTCA 
GAAGATAACA TTACAGAATC TCAACACATG CTAGTGGCTG AAATCATGGT GTCAGATATT 
GATGAGTGCC AACTGGGGGA GCACAGCTGT GGAGAGAATG CCAGCTGCAC AAATACAGAG 
GGAGGCTATA CCTGCATGTG TGCTGGACGC CTGTCTGAAC CAGGACTGAT TTGCCCTGAC 
TCTACTCCAC CCCCTCACCT CAGGGAAGAT GACCACCACT ATTCCGTAAG AAATAGTGAC 
TCTGAATGTC CCCTGTCCCA CGATGGGTAC TGCCTCCATG ATGGTGTGTG CATGTATATT 
GAAGCATTGG ACAAGTATGC ATGCAACTGT GTTGTTGGCT ACATCGGGGA GCGATGTCAG 
TACCGAGACC TGAAGTGGTG GGAACTGCGC CACGCTGGCC ACGGGCAGCA GCAGAAGGTC 
ATCGTGGTGG CTGTCTGCGT GGTGGTGCTT GTCATGCTGC TCCTCCTGAG CCTGTGGGGG 
GCCCACTACT ACAGGACTCA GAAGCTGCTA TCGAAAAACC CAAAGAATCC TTATGAGGAG 
TCGAGCAGAG ATGTGAGGAG TCGCAGGCCT GCTGACACTG AGGATGGGAT GTCCTCTTGC 
CCTCAACCTT GGGGTCAATG CAACCAACTT CATGGAGGCA GGAGCCCCAG TTATGTGGAA 
TGGGCACAGA GCAAGGCTGC TGGATTCCAG TATCCAGTGA TAAGGGCTCC TGTCCCCAGG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EGF ( XM_017007852.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016863341.1 Homo sapiens epidermal growth factor (EGF), transcript variant X10, mRNA.

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CloneID	OHu94957
Clone ID Related Accession (Same CDS sequence)	XM_017007852.1 , NM_001357021.2 , NM_001357021.1
Accession Version	XM_017007852.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3033bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(Human)
Product	pro-epidermal growth factor isoform X10
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCTCA CTCTTATCAT TCTGTTGCCA GTAGTTTCAA AATTTAGTTT TGTTAGTCTC 
TCAGCACCGC AGCACTGGAG CTGTCCTGAA GGTACTCTCG CAGGAAATGG GAATTCTACT 
TGTGTGGGTC CTGCACCCTT CTTAATTTTC TCCCATGGAA ATAGTATCTT TAGGATTGAC 
ACAGAAGGAA CCAATTATGA GCAATTGGTG GTGGATGCTG GTGTCTCAGT GATCATGGAT 
TTTCATTATA ATGAGAAAAG AATCTATTGG GTGGATTTAG AAAGACAACT TTTGCAAAGA 
GTTTTTCTGA ATGGGTCAAG GCAAGAGAGA GTATGTAATA TAGAGAAAAA TGTTTCTGGA 
ATGGCAATAA ATTGGATAAA TGAAGAAGTT ATTTGGTCAA ATCAACAGGA AGGAATCATT 
ACAGTAACAG ATATGAAAGG AAATAATTCC CACATTCTTT TAAGTGCTTT AAAATATCCT 
GCAAATGTAG CAGTTGATCC AGTAGAAAGG TTTATATTTT GGTCTTCAGA GGTGGCTGGA 
AGCCTTTATA GAGCAGATCT CGATGGTGTG GGAGTGAAGG CTCTGTTGGA GACATCAGAG 
AAAATAACAG CTGTGTCATT GGATGTGCTT GATAAGCGGC TGTTTTGGAT TCAGTACAAC 
AGAGAAGGAA GCAATTCTCT TATTTGCTCC TGTGATTATG ATGGAGGTTC TGTCCACATT 
AGTAAACATC CAACACAGCA TAATTTGTTT GCAATGTCCC TTTTTGGTGA CCGTATCTTC 
TATTCAACAT GGAAAATGAA GACAATTTGG ATAGCCAACA AACACACTGG AAAGGACATG 
GTTAGAATTA ACCTCCATTC ATCATTTGTA CCACTTGGTG AACTGAAAGT AGTGCATCCA 
CTTGCACAAC CCAAGGCAGA AGATGACACT TGGGAGCCTG ATGTTAATGA ATGTGCTTTT 
TGGAATCATG GCTGTACTCT TGGGTGTAAA AACACCCCTG GATCCTATTA CTGCACGTGC 
CCTGTAGGAT TTGTTCTGCT TCCTGATGGG AAACGATGTC ATCAACTTGT TTCCTGTCCA 
CGCAATGTGT CTGAATGCAG CCATGACTGT GTTCTGACAT CAGAAGGTCC CTTATGTTTC 
TGTCCTGAAG GCTCAGTGCT TGAGAGAGAT GGGAAAACAT GTAGCGGTTG TTCCTCACCC 
GATAATGGTG GATGTAGCCA GCTCTGCGTT CCTCTTAGCC CAGTATCCTG GGAATGTGAT 
TGCTTTCCTG GGTATGACCT ACAACTGGAT GAAAAAAGCT GTGCAGCTTC AGGACCACAA 
CCATTTTTGC TGTTTGCCAA TTCTCAAGAT ATTCGACACA TGCATTTTGA TGGAACAGAC 
TATGGAACTC TGCTCAGCCA GCAGATGGGA ATGGTTTATG CCCTAGATCA TGACCCTGTG 
GAAAATAAGA TATACTTTGC CCATACAGCC CTGAAGTGGA TAGAGAGAGC TAATATGGAT 
GGTTCCCAGC GAGAAAGGCT TATTGAGGAA GGAGTAGATG TGCCAGAAGG TCTTGCTGTG 
GACTGGATTG GCCGTAGATT CTATTGGACA GACAGAGGGA AATCTCTGAT TGGAAGGAGT 
GATTTAAATG GGAAACGTTC CAAAATAATC ACTAAGGAGA ACATCTCTCA ACCACGAGGA 
ATTGCTGTTC ATCCAATGGC CAAGAGATTA TTCTGGACTG ATACAGGGAT TAATCCACGA 
ATTGAAAGTT CTTCCCTCCA AGGCCTTGGC CGTCTGGTTA TAGCCAGCTC TGATCTAATC 
TGGCCCAGTG GAATAACGAT TGACTTCTTA ACTGACAAGT TGTACTGGTG CGATGCCAAG 
CAGTCTGTGA TTGAAATGGC CAATCTGGAT GGTTCAAAAC GCCGAAGACT TACCCAGAAT 
GATGTAGGTC ACCCATTTGC TGTAGCAGTG TTTGAGGATT ATGTGTGGTT CTCAGATTGG 
GCTATGCCAT CAGTAATGAG AGTAAACAAG AGGACTGGCA AAGATAGAGT ACGTCTCCAA 
GGCAGCATGC TGAAGCCCTC ATCACTGGTT GTGGTTCATC CATTGGCAAA ACCAGGAGCA 
GATCCCTGCT TATATCAAAA CGGAGGCTGT GAACATATTT GCAAAAAGAG GCTTGGAACT 
GCTTGGTGTT CGTGTCGTGA AGGTTTTATG AAAGCCTCAG ATGGGAAAAC GTGTCTGGCT 
CTGGATGGTC ATCAGCTGTT GGCAGGTGGT GAAGTTGATC TAAAGAACCA AGTAACACCA 
TTGGACATCT TGTCCAAGAC TAGAGTGTCA GAAGATAACA TTACAGAATC TCAACACATG 
CTAGTGGCTG AAATCATGGT GTCAGATATT GATGAGTGCC AACTGGGGGA GCACAGCTGT 
GGAGAGAATG CCAGCTGCAC AAATACAGAG GGAGGCTATA CCTGCATGTG TGCTGGACGC 
CTGTCTGAAC CAGGACTGAT TTGCCCTGAC TCTACTCCAC CCCCTCACCT CAGGGAAGAT 
GACCACCACT ATTCCGTAAG AAATAGTGAC TCTGAATGTC CCCTGTCCCA CGATGGGTAC 
TGCCTCCATG ATGGTGTGTG CATGTATATT GAAGCATTGG ACAAGTATGC ATGCAACTGT 
GTTGTTGGCT ACATCGGGGA GCGATGTCAG TACCGAGACC TGAAGTGGTG GGAACTGCGC 
CACGCTGGCC ACGGGCAGCA GCAGAAGGTC ATCGTGGTGG CTGTCTGCGT GGTGGTGCTT 
GTCATGCTGC TCCTCCTGAG CCTGTGGGGG GCCCACTACT ACAGGACTCA GAAGCTGCTA 
TCGAAAAACC CAAAGAATCC TTATGAGGAG TCGAGCAGAG ATGTGAGGAG TCGCAGGCCT 
GCTGACACTG AGGATGGGAT GTCCTCTTGC CCTCAACCTT GGGGTCAATG CAACCAACTT 
CATGGAGGCA GGAGCCCCAG TTATGTGGAA TGGGCACAGA GCAAGGCTGC TGGATTCCAG 
TATCCAGTGA TAAGGGCTCC TGTCCCCAGG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016863341.1
CDS	454..3486
Translation	MLLTLIILLPVVSKFSFVSLSAPQHWSCPEGTLAGNGNSTCVGPAPFLIFSHGNSIFRIDTEGTNYEQLVVDAGVSVIMDFHYNEKRIYWVDLERQLLQRVFLNGSRQERVCNIEKNVSGMAINWINEEVIWSNQQEGIITVTDMKGNNSHILLSALKYPANVAVDPVERFIFWSSEVAGSLYRADLDGVGVKALLETSEKITAVSLDVLDKRLFWIQYNREGSNSLICSCDYDGGSVHISKHPTQHNLFAMSLFGDRIFYSTWKMKTIWIANKHTGKDMVRINLHSSFVPLGELKVVHPLAQPKAEDDTWEPDVNECAFWNHGCTLGCKNTPGSYYCTCPVGFVLLPDGKRCHQLVSCPRNVSECSHDCVLTSEGPLCFCPEGSVLERDGKTCSGCSSPDNGGCSQLCVPLSPVSWECDCFPGYDLQLDEKSCAASGPQPFLLFANSQDIRHMHFDGTDYGTLLSQQMGMVYALDHDPVENKIYFAHTALKWIERANMDGSQRERLIEEGVDVPEGLAVDWIGRRFYWTDRGKSLIGRSDLNGKRSKIITKENISQPRGIAVHPMAKRLFWTDTGINPRIESSSLQGLGRLVIASSDLIWPSGITIDFLTDKLYWCDAKQSVIEMANLDGSKRRRLTQNDVGHPFAVAVFEDYVWFSDWAMPSVMRVNKRTGKDRVRLQGSMLKPSSLVVVHPLAKPGADPCLYQNGGCEHICKKRLGTAWCSCREGFMKASDGKTCLALDGHQLLAGGEVDLKNQVTPLDILSKTRVSEDNITESQHMLVAEIMVSDIDECQLGEHSCGENASCTNTEGGYTCMCAGRLSEPGLICPDSTPPPHLREDDHHYSVRNSDSECPLSHDGYCLHDGVCMYIEALDKYACNCVVGYIGERCQYRDLKWWELRHAGHGQQQKVIVVAVCVVVLVMLLLLSLWGAHYYRTQKLLSKNPKNPYEESSRDVRSRRPADTEDGMSSCPQPWGQCNQLHGGRSPSYVEWAQSKAAGFQYPVIRAPVPR

Target ORF information:

RefSeq Version	XM_017007852.1
Organism	Homo sapiens(Human)
Definition	Homo sapiens epidermal growth factor (EGF), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017007852.1

ORF Insert Sequence:

ATGCTGCTCA CTCTTATCAT TCTGTTGCCA GTAGTTTCAA AATTTAGTTT TGTTAGTCTC 
TCAGCACCGC AGCACTGGAG CTGTCCTGAA GGTACTCTCG CAGGAAATGG GAATTCTACT 
TGTGTGGGTC CTGCACCCTT CTTAATTTTC TCCCATGGAA ATAGTATCTT TAGGATTGAC 
ACAGAAGGAA CCAATTATGA GCAATTGGTG GTGGATGCTG GTGTCTCAGT GATCATGGAT 
TTTCATTATA ATGAGAAAAG AATCTATTGG GTGGATTTAG AAAGACAACT TTTGCAAAGA 
GTTTTTCTGA ATGGGTCAAG GCAAGAGAGA GTATGTAATA TAGAGAAAAA TGTTTCTGGA 
ATGGCAATAA ATTGGATAAA TGAAGAAGTT ATTTGGTCAA ATCAACAGGA AGGAATCATT 
ACAGTAACAG ATATGAAAGG AAATAATTCC CACATTCTTT TAAGTGCTTT AAAATATCCT 
GCAAATGTAG CAGTTGATCC AGTAGAAAGG TTTATATTTT GGTCTTCAGA GGTGGCTGGA 
AGCCTTTATA GAGCAGATCT CGATGGTGTG GGAGTGAAGG CTCTGTTGGA GACATCAGAG 
AAAATAACAG CTGTGTCATT GGATGTGCTT GATAAGCGGC TGTTTTGGAT TCAGTACAAC 
AGAGAAGGAA GCAATTCTCT TATTTGCTCC TGTGATTATG ATGGAGGTTC TGTCCACATT 
AGTAAACATC CAACACAGCA TAATTTGTTT GCAATGTCCC TTTTTGGTGA CCGTATCTTC 
TATTCAACAT GGAAAATGAA GACAATTTGG ATAGCCAACA AACACACTGG AAAGGACATG 
GTTAGAATTA ACCTCCATTC ATCATTTGTA CCACTTGGTG AACTGAAAGT AGTGCATCCA 
CTTGCACAAC CCAAGGCAGA AGATGACACT TGGGAGCCTG ATGTTAATGA ATGTGCTTTT 
TGGAATCATG GCTGTACTCT TGGGTGTAAA AACACCCCTG GATCCTATTA CTGCACGTGC 
CCTGTAGGAT TTGTTCTGCT TCCTGATGGG AAACGATGTC ATCAACTTGT TTCCTGTCCA 
CGCAATGTGT CTGAATGCAG CCATGACTGT GTTCTGACAT CAGAAGGTCC CTTATGTTTC 
TGTCCTGAAG GCTCAGTGCT TGAGAGAGAT GGGAAAACAT GTAGCGGTTG TTCCTCACCC 
GATAATGGTG GATGTAGCCA GCTCTGCGTT CCTCTTAGCC CAGTATCCTG GGAATGTGAT 
TGCTTTCCTG GGTATGACCT ACAACTGGAT GAAAAAAGCT GTGCAGCTTC AGGACCACAA 
CCATTTTTGC TGTTTGCCAA TTCTCAAGAT ATTCGACACA TGCATTTTGA TGGAACAGAC 
TATGGAACTC TGCTCAGCCA GCAGATGGGA ATGGTTTATG CCCTAGATCA TGACCCTGTG 
GAAAATAAGA TATACTTTGC CCATACAGCC CTGAAGTGGA TAGAGAGAGC TAATATGGAT 
GGTTCCCAGC GAGAAAGGCT TATTGAGGAA GGAGTAGATG TGCCAGAAGG TCTTGCTGTG 
GACTGGATTG GCCGTAGATT CTATTGGACA GACAGAGGGA AATCTCTGAT TGGAAGGAGT 
GATTTAAATG GGAAACGTTC CAAAATAATC ACTAAGGAGA ACATCTCTCA ACCACGAGGA 
ATTGCTGTTC ATCCAATGGC CAAGAGATTA TTCTGGACTG ATACAGGGAT TAATCCACGA 
ATTGAAAGTT CTTCCCTCCA AGGCCTTGGC CGTCTGGTTA TAGCCAGCTC TGATCTAATC 
TGGCCCAGTG GAATAACGAT TGACTTCTTA ACTGACAAGT TGTACTGGTG CGATGCCAAG 
CAGTCTGTGA TTGAAATGGC CAATCTGGAT GGTTCAAAAC GCCGAAGACT TACCCAGAAT 
GATGTAGGTC ACCCATTTGC TGTAGCAGTG TTTGAGGATT ATGTGTGGTT CTCAGATTGG 
GCTATGCCAT CAGTAATGAG AGTAAACAAG AGGACTGGCA AAGATAGAGT ACGTCTCCAA 
GGCAGCATGC TGAAGCCCTC ATCACTGGTT GTGGTTCATC CATTGGCAAA ACCAGGAGCA 
GATCCCTGCT TATATCAAAA CGGAGGCTGT GAACATATTT GCAAAAAGAG GCTTGGAACT 
GCTTGGTGTT CGTGTCGTGA AGGTTTTATG AAAGCCTCAG ATGGGAAAAC GTGTCTGGCT 
CTGGATGGTC ATCAGCTGTT GGCAGGTGGT GAAGTTGATC TAAAGAACCA AGTAACACCA 
TTGGACATCT TGTCCAAGAC TAGAGTGTCA GAAGATAACA TTACAGAATC TCAACACATG 
CTAGTGGCTG AAATCATGGT GTCAGATATT GATGAGTGCC AACTGGGGGA GCACAGCTGT 
GGAGAGAATG CCAGCTGCAC AAATACAGAG GGAGGCTATA CCTGCATGTG TGCTGGACGC 
CTGTCTGAAC CAGGACTGAT TTGCCCTGAC TCTACTCCAC CCCCTCACCT CAGGGAAGAT 
GACCACCACT ATTCCGTAAG AAATAGTGAC TCTGAATGTC CCCTGTCCCA CGATGGGTAC 
TGCCTCCATG ATGGTGTGTG CATGTATATT GAAGCATTGG ACAAGTATGC ATGCAACTGT 
GTTGTTGGCT ACATCGGGGA GCGATGTCAG TACCGAGACC TGAAGTGGTG GGAACTGCGC 
CACGCTGGCC ACGGGCAGCA GCAGAAGGTC ATCGTGGTGG CTGTCTGCGT GGTGGTGCTT 
GTCATGCTGC TCCTCCTGAG CCTGTGGGGG GCCCACTACT ACAGGACTCA GAAGCTGCTA 
TCGAAAAACC CAAAGAATCC TTATGAGGAG TCGAGCAGAG ATGTGAGGAG TCGCAGGCCT 
GCTGACACTG AGGATGGGAT GTCCTCTTGC CCTCAACCTT GGGGTCAATG CAACCAACTT 
CATGGAGGCA GGAGCCCCAG TTATGTGGAA TGGGCACAGA GCAAGGCTGC TGGATTCCAG 
TATCCAGTGA TAAGGGCTCC TGTCCCCAGG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EGF ( NM_001178131.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001171602.1 Homo sapiens epidermal growth factor (EGF), transcript variant 3, mRNA.

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CloneID	OHu15827
Clone ID Related Accession (Same CDS sequence)	NM_001178131.3 , NM_001178131.2
Accession Version	NM_001178131.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3498bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-03
Organism	Homo sapiens(human)
Product	pro-epidermal growth factor isoform 3 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC005509.1, AK299306.1, AC004050.1 and CA437074.1. On May 31, 2019 this sequence version replaced NM_001178131.2. Transcript Variant: This variant (3) lacks an in-frame exon in the coding region, compared to variant 1. The encoded isoform (3) is shorter than isoform 1. This isoform (3) may undergo proteolytic processing similar to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK299306.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968832, SAMEA1970526 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCTGCTCA CTCTTATCAT TCTGTTGCCA GTAGTTTCAA AATTTAGTTT TGTTAGTCTC 
TCAGCACCGC AGCACTGGAG CTGTCCTGAA GGTACTCTCG CAGGAAATGG GAATTCTACT 
TGTGTGGGTC CTGCACCCTT CTTAATTTTC TCCCATGGAA ATAGTATCTT TAGGATTGAC 
ACAGAAGGAA CCAATTATGA GCAATTGGTG GTGGATGCTG GTGTCTCAGT GATCATGGAT 
TTTCATTATA ATGAGAAAAG AATCTATTGG GTGGATTTAG AAAGACAACT TTTGCAAAGA 
GTTTTTCTGA ATGGGTCAAG GCAAGAGAGA GTATGTAATA TAGAGAAAAA TGTTTCTGGA 
ATGGCAATAA ATTGGATAAA TGAAGAAGTT ATTTGGTCAA ATCAACAGGA AGGAATCATT 
ACAGTAACAG ATATGAAAGG AAATAATTCC CACATTCTTT TAAGTGCTTT AAAATATCCT 
GCAAATGTAG CAGTTGATCC AGTAGAAAGG TTTATATTTT GGTCTTCAGA GGTGGCTGGA 
AGCCTTTATA GAGCAGATCT CGATGGTGTG GGAGTGAAGG CTCTGTTGGA GACATCAGAG 
AAAATAACAG CTGTGTCATT GGATGTGCTT GATAAGCGGC TGTTTTGGAT TCAGTACAAC 
AGAGAAGGAA GCAATTCTCT TATTTGCTCC TGTGATTATG ATGGAGGTTC TGTCCACATT 
AGTAAACATC CAACACAGCA TAATTTGTTT GCAATGTCCC TTTTTGGTGA CCGTATCTTC 
TATTCAACAT GGAAAATGAA GACAATTTGG ATAGCCAACA AACACACTGG AAAGGACATG 
GTTAGAATTA ACCTCCATTC ATCATTTGTA CCACTTGGTG AACTGAAAGT AGTGCATCCA 
CTTGCACAAC CCAAGGCAGA AGATGACACT TGGGAGCCTG ATGTTAATGA ATGTGCTTTT 
TGGAATCATG GCTGTACTCT TGGGTGTAAA AACACCCCTG GATCCTATTA CTGCACGTGC 
CCTGTAGGAT TTGTTCTGCT TCCTGATGGG AAACGATGTC ATCAACTTGT TTCCTGTCCA 
CGCAATGTGT CTGAATGCAG CCATGACTGT GTTCTGACAT CAGAAGGTCC CTTATGTTTC 
TGTCCTGAAG GCTCAGTGCT TGAGAGAGAT GGGAAAACAT GTAGCGGTTG TTCCTCACCC 
GATAATGGTG GATGTAGCCA GCTCTGCGTT CCTCTTAGCC CAGTATCCTG GGAATGTGAT 
TGCTTTCCTG GGTATGACCT ACAACTGGAT GAAAAAAGCT GTGCAGCTTC AGGACCACAA 
CCATTTTTGC TGTTTGCCAA TTCTCAAGAT ATTCGACACA TGCATTTTGA TGGAACAGAC 
TATGGAACTC TGCTCAGCCA GCAGATGGGA ATGGTTTATG CCCTAGATCA TGACCCTGTG 
GAAAATAAGA TATACTTTGC CCATACAGCC CTGAAGTGGA TAGAGAGAGC TAATATGGAT 
GGTTCCCAGC GAGAAAGGCT TATTGAGGAA GGAGTAGATG TGCCAGAAGG TCTTGCTGTG 
GACTGGATTG GCCGTAGATT CTATTGGACA GACAGAGGGA AATCTCTGAT TGGAAGGAGT 
GATTTAAATG GGAAACGTTC CAAAATAATC ACTAAGGAGA ACATCTCTCA ACCACGAGGA 
ATTGCTGTTC ATCCAATGGC CAAGAGATTA TTCTGGACTG ATACAGGGAT TAATCCACGA 
ATTGAAAGTT CTTCCCTCCA AGGCCTTGGC CGTCTGGTTA TAGCCAGCTC TGATCTAATC 
TGGCCCAGTG GAATAACGAT TGACTTCTTA ACTGACAAGT TGTACTGGTG CGATGCCAAG 
CAGTCTGTGA TTGAAATGGC CAATCTGGAT GGTTCAAAAC GCCGAAGACT TACCCAGAAT 
GATGTAGGTC ACCCATTTGC TGTAGCAGTG TTTGAGGATT ATGTGTGGTT CTCAGATTGG 
GCTATGCCAT CAGTAATGAG AGTAAACAAG AGGACTGGCA AAGATAGAGT ACGTCTCCAA 
GGCAGCATGC TGAAGCCCTC ATCACTGGTT GTGGTTCATC CATTGGCAAA ACCAGGAGCA 
GATCCCTGCT TATATCAAAA CGGAGGCTGT GAACATATTT GCAAAAAGAG GCTTGGAACT 
GCTTGGTGTT CGTGTCGTGA AGGTTTTATG AAAGCCTCAG ATGGGAAAAC GTGTCTGGCT 
CTGGATGGTC ATCAGCTGTT GGCAGGTGGT GAAGTTGATC TAAAGAACCA AGTAACACCA 
TTGGACATCT TGTCCAAGAC TAGAGTGTCA GAAGATAACA TTACAGAATC TCAACACATG 
CTAGTGGCTG AAATCATGGT GTCAGATCAA GATGACTGTG CTCCTGTGGG ATGCAGCATG 
TATGCTCGGT GTATTTCAGA GGGAGAGGAT GCCACATGTC AGTGTTTGAA AGGATTTGCT 
GGGGATGGAA AACTATGTTC TGATATAGAT GAATGTGAGA TGGGTGTCCC AGTGTGCCCC 
CCTGCCTCCT CCAAGTGCAT CAACACCGAA GGTGGTTATG TCTGCCGGTG CTCAGAAGGC 
TACCAAGGAG ATGGGATTCA CTGTCTTGAT ATTGATGAGT GCCAACTGGG GGAGCACAGC 
TGTGGAGAGA ATGCCAGCTG CACAAATACA GAGGGAGGCT ATACCTGCAT GTGTGCTGGA 
CGCCTGTCTG AACCAGGACT GATTTGCCCT GACTCTACTC CACCCCCTCA CCTCAGGGAA 
GATGACCACC ACTATTCCGT AAGAAATAGT GACTCTGAAT GTCCCCTGTC CCACGATGGG 
TACTGCCTCC ATGATGGTGT GTGCATGTAT ATTGAAGCAT TGGACAAGTA TGCATGCAAC 
TGTGTTGTTG GCTACATCGG GGAGCGATGT CAGTACCGAG ACCTGAAGTG GTGGGAACTG 
CGCCACGCTG GCCACGGGCA GCAGCAGAAG GTCATCGTGG TGGCTGTCTG CGTGGTGGTG 
CTTGTCATGC TGCTCCTCCT GAGCCTGTGG GGGGCCCACT ACTACAGGAC TCAGAAGCTG 
CTATCGAAAA ACCCAAAGAA TCCTTATGAG GAGTCGAGCA GAGATGTGAG GAGTCGCAGG 
CCTGCTGACA CTGAGGATGG GATGTCCTCT TGCCCTCAAC CTTGGTTTGT GGTTATAAAA 
GAACACCAAG ACCTCAAGAA TGGGGGTCAA CCAGTGGCTG GTGAGGATGG CCAGGCAGCA 
GATGGGTCAA TGCAACCAAC TTCATGGAGG CAGGAGCCCC AGTTATGTGG AATGGGCACA 
GAGCAAGGCT GCTGGATTCC AGTATCCAGT GATAAGGGCT CCTGTCCCCA GGTAATGGAG 
CGAAGCTTTC ATATGCCCTC CTATGGGACA CAGACCCTTG AAGGGGGTGT CGAGAAGCCC 
CATTCTCTCC TATCAGCTAA CCCATTATGG CAACAAAGGG CCCTGGACCC ACCACACCAA 
ATGGAGCTGA CTCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001171602.1
CDS	454..3951
Translation	MLLTLIILLPVVSKFSFVSLSAPQHWSCPEGTLAGNGNSTCVGPAPFLIFSHGNSIFRIDTEGTNYEQLVVDAGVSVIMDFHYNEKRIYWVDLERQLLQRVFLNGSRQERVCNIEKNVSGMAINWINEEVIWSNQQEGIITVTDMKGNNSHILLSALKYPANVAVDPVERFIFWSSEVAGSLYRADLDGVGVKALLETSEKITAVSLDVLDKRLFWIQYNREGSNSLICSCDYDGGSVHISKHPTQHNLFAMSLFGDRIFYSTWKMKTIWIANKHTGKDMVRINLHSSFVPLGELKVVHPLAQPKAEDDTWEPDVNECAFWNHGCTLGCKNTPGSYYCTCPVGFVLLPDGKRCHQLVSCPRNVSECSHDCVLTSEGPLCFCPEGSVLERDGKTCSGCSSPDNGGCSQLCVPLSPVSWECDCFPGYDLQLDEKSCAASGPQPFLLFANSQDIRHMHFDGTDYGTLLSQQMGMVYALDHDPVENKIYFAHTALKWIERANMDGSQRERLIEEGVDVPEGLAVDWIGRRFYWTDRGKSLIGRSDLNGKRSKIITKENISQPRGIAVHPMAKRLFWTDTGINPRIESSSLQGLGRLVIASSDLIWPSGITIDFLTDKLYWCDAKQSVIEMANLDGSKRRRLTQNDVGHPFAVAVFEDYVWFSDWAMPSVMRVNKRTGKDRVRLQGSMLKPSSLVVVHPLAKPGADPCLYQNGGCEHICKKRLGTAWCSCREGFMKASDGKTCLALDGHQLLAGGEVDLKNQVTPLDILSKTRVSEDNITESQHMLVAEIMVSDQDDCAPVGCSMYARCISEGEDATCQCLKGFAGDGKLCSDIDECEMGVPVCPPASSKCINTEGGYVCRCSEGYQGDGIHCLDIDECQLGEHSCGENASCTNTEGGYTCMCAGRLSEPGLICPDSTPPPHLREDDHHYSVRNSDSECPLSHDGYCLHDGVCMYIEALDKYACNCVVGYIGERCQYRDLKWWELRHAGHGQQQKVIVVAVCVVVLVMLLLLSLWGAHYYRTQKLLSKNPKNPYEESSRDVRSRRPADTEDGMSSCPQPWFVVIKEHQDLKNGGQPVAGEDGQAADGSMQPTSWRQEPQLCGMGTEQGCWIPVSSDKGSCPQVMERSFHMPSYGTQTLEGGVEKPHSLLSANPLWQQRALDPPHQMELTQ

Target ORF information:

RefSeq Version	NM_001178131.3
Organism	Homo sapiens(human)
Definition	Homo sapiens epidermal growth factor (EGF), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001178131.3

ORF Insert Sequence:

ATGCTGCTCA CTCTTATCAT TCTGTTGCCA GTAGTTTCAA AATTTAGTTT TGTTAGTCTC 
TCAGCACCGC AGCACTGGAG CTGTCCTGAA GGTACTCTCG CAGGAAATGG GAATTCTACT 
TGTGTGGGTC CTGCACCCTT CTTAATTTTC TCCCATGGAA ATAGTATCTT TAGGATTGAC 
ACAGAAGGAA CCAATTATGA GCAATTGGTG GTGGATGCTG GTGTCTCAGT GATCATGGAT 
TTTCATTATA ATGAGAAAAG AATCTATTGG GTGGATTTAG AAAGACAACT TTTGCAAAGA 
GTTTTTCTGA ATGGGTCAAG GCAAGAGAGA GTATGTAATA TAGAGAAAAA TGTTTCTGGA 
ATGGCAATAA ATTGGATAAA TGAAGAAGTT ATTTGGTCAA ATCAACAGGA AGGAATCATT 
ACAGTAACAG ATATGAAAGG AAATAATTCC CACATTCTTT TAAGTGCTTT AAAATATCCT 
GCAAATGTAG CAGTTGATCC AGTAGAAAGG TTTATATTTT GGTCTTCAGA GGTGGCTGGA 
AGCCTTTATA GAGCAGATCT CGATGGTGTG GGAGTGAAGG CTCTGTTGGA GACATCAGAG 
AAAATAACAG CTGTGTCATT GGATGTGCTT GATAAGCGGC TGTTTTGGAT TCAGTACAAC 
AGAGAAGGAA GCAATTCTCT TATTTGCTCC TGTGATTATG ATGGAGGTTC TGTCCACATT 
AGTAAACATC CAACACAGCA TAATTTGTTT GCAATGTCCC TTTTTGGTGA CCGTATCTTC 
TATTCAACAT GGAAAATGAA GACAATTTGG ATAGCCAACA AACACACTGG AAAGGACATG 
GTTAGAATTA ACCTCCATTC ATCATTTGTA CCACTTGGTG AACTGAAAGT AGTGCATCCA 
CTTGCACAAC CCAAGGCAGA AGATGACACT TGGGAGCCTG ATGTTAATGA ATGTGCTTTT 
TGGAATCATG GCTGTACTCT TGGGTGTAAA AACACCCCTG GATCCTATTA CTGCACGTGC 
CCTGTAGGAT TTGTTCTGCT TCCTGATGGG AAACGATGTC ATCAACTTGT TTCCTGTCCA 
CGCAATGTGT CTGAATGCAG CCATGACTGT GTTCTGACAT CAGAAGGTCC CTTATGTTTC 
TGTCCTGAAG GCTCAGTGCT TGAGAGAGAT GGGAAAACAT GTAGCGGTTG TTCCTCACCC 
GATAATGGTG GATGTAGCCA GCTCTGCGTT CCTCTTAGCC CAGTATCCTG GGAATGTGAT 
TGCTTTCCTG GGTATGACCT ACAACTGGAT GAAAAAAGCT GTGCAGCTTC AGGACCACAA 
CCATTTTTGC TGTTTGCCAA TTCTCAAGAT ATTCGACACA TGCATTTTGA TGGAACAGAC 
TATGGAACTC TGCTCAGCCA GCAGATGGGA ATGGTTTATG CCCTAGATCA TGACCCTGTG 
GAAAATAAGA TATACTTTGC CCATACAGCC CTGAAGTGGA TAGAGAGAGC TAATATGGAT 
GGTTCCCAGC GAGAAAGGCT TATTGAGGAA GGAGTAGATG TGCCAGAAGG TCTTGCTGTG 
GACTGGATTG GCCGTAGATT CTATTGGACA GACAGAGGGA AATCTCTGAT TGGAAGGAGT 
GATTTAAATG GGAAACGTTC CAAAATAATC ACTAAGGAGA ACATCTCTCA ACCACGAGGA 
ATTGCTGTTC ATCCAATGGC CAAGAGATTA TTCTGGACTG ATACAGGGAT TAATCCACGA 
ATTGAAAGTT CTTCCCTCCA AGGCCTTGGC CGTCTGGTTA TAGCCAGCTC TGATCTAATC 
TGGCCCAGTG GAATAACGAT TGACTTCTTA ACTGACAAGT TGTACTGGTG CGATGCCAAG 
CAGTCTGTGA TTGAAATGGC CAATCTGGAT GGTTCAAAAC GCCGAAGACT TACCCAGAAT 
GATGTAGGTC ACCCATTTGC TGTAGCAGTG TTTGAGGATT ATGTGTGGTT CTCAGATTGG 
GCTATGCCAT CAGTAATGAG AGTAAACAAG AGGACTGGCA AAGATAGAGT ACGTCTCCAA 
GGCAGCATGC TGAAGCCCTC ATCACTGGTT GTGGTTCATC CATTGGCAAA ACCAGGAGCA 
GATCCCTGCT TATATCAAAA CGGAGGCTGT GAACATATTT GCAAAAAGAG GCTTGGAACT 
GCTTGGTGTT CGTGTCGTGA AGGTTTTATG AAAGCCTCAG ATGGGAAAAC GTGTCTGGCT 
CTGGATGGTC ATCAGCTGTT GGCAGGTGGT GAAGTTGATC TAAAGAACCA AGTAACACCA 
TTGGACATCT TGTCCAAGAC TAGAGTGTCA GAAGATAACA TTACAGAATC TCAACACATG 
CTAGTGGCTG AAATCATGGT GTCAGATCAA GATGACTGTG CTCCTGTGGG ATGCAGCATG 
TATGCTCGGT GTATTTCAGA GGGAGAGGAT GCCACATGTC AGTGTTTGAA AGGATTTGCT 
GGGGATGGAA AACTATGTTC TGATATAGAT GAATGTGAGA TGGGTGTCCC AGTGTGCCCC 
CCTGCCTCCT CCAAGTGCAT CAACACCGAA GGTGGTTATG TCTGCCGGTG CTCAGAAGGC 
TACCAAGGAG ATGGGATTCA CTGTCTTGAT ATTGATGAGT GCCAACTGGG GGAGCACAGC 
TGTGGAGAGA ATGCCAGCTG CACAAATACA GAGGGAGGCT ATACCTGCAT GTGTGCTGGA 
CGCCTGTCTG AACCAGGACT GATTTGCCCT GACTCTACTC CACCCCCTCA CCTCAGGGAA 
GATGACCACC ACTATTCCGT AAGAAATAGT GACTCTGAAT GTCCCCTGTC CCACGATGGG 
TACTGCCTCC ATGATGGTGT GTGCATGTAT ATTGAAGCAT TGGACAAGTA TGCATGCAAC 
TGTGTTGTTG GCTACATCGG GGAGCGATGT CAGTACCGAG ACCTGAAGTG GTGGGAACTG 
CGCCACGCTG GCCACGGGCA GCAGCAGAAG GTCATCGTGG TGGCTGTCTG CGTGGTGGTG 
CTTGTCATGC TGCTCCTCCT GAGCCTGTGG GGGGCCCACT ACTACAGGAC TCAGAAGCTG 
CTATCGAAAA ACCCAAAGAA TCCTTATGAG GAGTCGAGCA GAGATGTGAG GAGTCGCAGG 
CCTGCTGACA CTGAGGATGG GATGTCCTCT TGCCCTCAAC CTTGGTTTGT GGTTATAAAA 
GAACACCAAG ACCTCAAGAA TGGGGGTCAA CCAGTGGCTG GTGAGGATGG CCAGGCAGCA 
GATGGGTCAA TGCAACCAAC TTCATGGAGG CAGGAGCCCC AGTTATGTGG AATGGGCACA 
GAGCAAGGCT GCTGGATTCC AGTATCCAGT GATAAGGGCT CCTGTCCCCA GGTAATGGAG 
CGAAGCTTTC ATATGCCCTC CTATGGGACA CAGACCCTTG AAGGGGGTGT CGAGAAGCCC 
CATTCTCTCC TATCAGCTAA CCCATTATGG CAACAAAGGG CCCTGGACCC ACCACACCAA 
ATGGAGCTGA CTCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EGF ( NM_001178131.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001171602.1 Homo sapiens epidermal growth factor (EGF), transcript variant 3, mRNA.

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CloneID	OHu15827
Clone ID Related Accession (Same CDS sequence)	NM_001178131.3 , NM_001178131.2
Accession Version	NM_001178131.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3498bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-24
Organism	Homo sapiens(human)
Product	pro-epidermal growth factor isoform 3 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK299306.1, AC005509.1, AC004050.1 and CA437074.1. On Jan 8, 2016 this sequence version replaced NM_001178131.1. Transcript Variant: This variant (3) lacks an in-frame exon in the coding region, compared to variant 1. The encoded isoform (3) is shorter than isoform 1. This isoform (3) may undergo proteolytic processing similar to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK299306.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968832, SAMEA1970526 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCTGCTCA CTCTTATCAT TCTGTTGCCA GTAGTTTCAA AATTTAGTTT TGTTAGTCTC 
TCAGCACCGC AGCACTGGAG CTGTCCTGAA GGTACTCTCG CAGGAAATGG GAATTCTACT 
TGTGTGGGTC CTGCACCCTT CTTAATTTTC TCCCATGGAA ATAGTATCTT TAGGATTGAC 
ACAGAAGGAA CCAATTATGA GCAATTGGTG GTGGATGCTG GTGTCTCAGT GATCATGGAT 
TTTCATTATA ATGAGAAAAG AATCTATTGG GTGGATTTAG AAAGACAACT TTTGCAAAGA 
GTTTTTCTGA ATGGGTCAAG GCAAGAGAGA GTATGTAATA TAGAGAAAAA TGTTTCTGGA 
ATGGCAATAA ATTGGATAAA TGAAGAAGTT ATTTGGTCAA ATCAACAGGA AGGAATCATT 
ACAGTAACAG ATATGAAAGG AAATAATTCC CACATTCTTT TAAGTGCTTT AAAATATCCT 
GCAAATGTAG CAGTTGATCC AGTAGAAAGG TTTATATTTT GGTCTTCAGA GGTGGCTGGA 
AGCCTTTATA GAGCAGATCT CGATGGTGTG GGAGTGAAGG CTCTGTTGGA GACATCAGAG 
AAAATAACAG CTGTGTCATT GGATGTGCTT GATAAGCGGC TGTTTTGGAT TCAGTACAAC 
AGAGAAGGAA GCAATTCTCT TATTTGCTCC TGTGATTATG ATGGAGGTTC TGTCCACATT 
AGTAAACATC CAACACAGCA TAATTTGTTT GCAATGTCCC TTTTTGGTGA CCGTATCTTC 
TATTCAACAT GGAAAATGAA GACAATTTGG ATAGCCAACA AACACACTGG AAAGGACATG 
GTTAGAATTA ACCTCCATTC ATCATTTGTA CCACTTGGTG AACTGAAAGT AGTGCATCCA 
CTTGCACAAC CCAAGGCAGA AGATGACACT TGGGAGCCTG ATGTTAATGA ATGTGCTTTT 
TGGAATCATG GCTGTACTCT TGGGTGTAAA AACACCCCTG GATCCTATTA CTGCACGTGC 
CCTGTAGGAT TTGTTCTGCT TCCTGATGGG AAACGATGTC ATCAACTTGT TTCCTGTCCA 
CGCAATGTGT CTGAATGCAG CCATGACTGT GTTCTGACAT CAGAAGGTCC CTTATGTTTC 
TGTCCTGAAG GCTCAGTGCT TGAGAGAGAT GGGAAAACAT GTAGCGGTTG TTCCTCACCC 
GATAATGGTG GATGTAGCCA GCTCTGCGTT CCTCTTAGCC CAGTATCCTG GGAATGTGAT 
TGCTTTCCTG GGTATGACCT ACAACTGGAT GAAAAAAGCT GTGCAGCTTC AGGACCACAA 
CCATTTTTGC TGTTTGCCAA TTCTCAAGAT ATTCGACACA TGCATTTTGA TGGAACAGAC 
TATGGAACTC TGCTCAGCCA GCAGATGGGA ATGGTTTATG CCCTAGATCA TGACCCTGTG 
GAAAATAAGA TATACTTTGC CCATACAGCC CTGAAGTGGA TAGAGAGAGC TAATATGGAT 
GGTTCCCAGC GAGAAAGGCT TATTGAGGAA GGAGTAGATG TGCCAGAAGG TCTTGCTGTG 
GACTGGATTG GCCGTAGATT CTATTGGACA GACAGAGGGA AATCTCTGAT TGGAAGGAGT 
GATTTAAATG GGAAACGTTC CAAAATAATC ACTAAGGAGA ACATCTCTCA ACCACGAGGA 
ATTGCTGTTC ATCCAATGGC CAAGAGATTA TTCTGGACTG ATACAGGGAT TAATCCACGA 
ATTGAAAGTT CTTCCCTCCA AGGCCTTGGC CGTCTGGTTA TAGCCAGCTC TGATCTAATC 
TGGCCCAGTG GAATAACGAT TGACTTCTTA ACTGACAAGT TGTACTGGTG CGATGCCAAG 
CAGTCTGTGA TTGAAATGGC CAATCTGGAT GGTTCAAAAC GCCGAAGACT TACCCAGAAT 
GATGTAGGTC ACCCATTTGC TGTAGCAGTG TTTGAGGATT ATGTGTGGTT CTCAGATTGG 
GCTATGCCAT CAGTAATGAG AGTAAACAAG AGGACTGGCA AAGATAGAGT ACGTCTCCAA 
GGCAGCATGC TGAAGCCCTC ATCACTGGTT GTGGTTCATC CATTGGCAAA ACCAGGAGCA 
GATCCCTGCT TATATCAAAA CGGAGGCTGT GAACATATTT GCAAAAAGAG GCTTGGAACT 
GCTTGGTGTT CGTGTCGTGA AGGTTTTATG AAAGCCTCAG ATGGGAAAAC GTGTCTGGCT 
CTGGATGGTC ATCAGCTGTT GGCAGGTGGT GAAGTTGATC TAAAGAACCA AGTAACACCA 
TTGGACATCT TGTCCAAGAC TAGAGTGTCA GAAGATAACA TTACAGAATC TCAACACATG 
CTAGTGGCTG AAATCATGGT GTCAGATCAA GATGACTGTG CTCCTGTGGG ATGCAGCATG 
TATGCTCGGT GTATTTCAGA GGGAGAGGAT GCCACATGTC AGTGTTTGAA AGGATTTGCT 
GGGGATGGAA AACTATGTTC TGATATAGAT GAATGTGAGA TGGGTGTCCC AGTGTGCCCC 
CCTGCCTCCT CCAAGTGCAT CAACACCGAA GGTGGTTATG TCTGCCGGTG CTCAGAAGGC 
TACCAAGGAG ATGGGATTCA CTGTCTTGAT ATTGATGAGT GCCAACTGGG GGAGCACAGC 
TGTGGAGAGA ATGCCAGCTG CACAAATACA GAGGGAGGCT ATACCTGCAT GTGTGCTGGA 
CGCCTGTCTG AACCAGGACT GATTTGCCCT GACTCTACTC CACCCCCTCA CCTCAGGGAA 
GATGACCACC ACTATTCCGT AAGAAATAGT GACTCTGAAT GTCCCCTGTC CCACGATGGG 
TACTGCCTCC ATGATGGTGT GTGCATGTAT ATTGAAGCAT TGGACAAGTA TGCATGCAAC 
TGTGTTGTTG GCTACATCGG GGAGCGATGT CAGTACCGAG ACCTGAAGTG GTGGGAACTG 
CGCCACGCTG GCCACGGGCA GCAGCAGAAG GTCATCGTGG TGGCTGTCTG CGTGGTGGTG 
CTTGTCATGC TGCTCCTCCT GAGCCTGTGG GGGGCCCACT ACTACAGGAC TCAGAAGCTG 
CTATCGAAAA ACCCAAAGAA TCCTTATGAG GAGTCGAGCA GAGATGTGAG GAGTCGCAGG 
CCTGCTGACA CTGAGGATGG GATGTCCTCT TGCCCTCAAC CTTGGTTTGT GGTTATAAAA 
GAACACCAAG ACCTCAAGAA TGGGGGTCAA CCAGTGGCTG GTGAGGATGG CCAGGCAGCA 
GATGGGTCAA TGCAACCAAC TTCATGGAGG CAGGAGCCCC AGTTATGTGG AATGGGCACA 
GAGCAAGGCT GCTGGATTCC AGTATCCAGT GATAAGGGCT CCTGTCCCCA GGTAATGGAG 
CGAAGCTTTC ATATGCCCTC CTATGGGACA CAGACCCTTG AAGGGGGTGT CGAGAAGCCC 
CATTCTCTCC TATCAGCTAA CCCATTATGG CAACAAAGGG CCCTGGACCC ACCACACCAA 
ATGGAGCTGA CTCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001171602.1
CDS	453..3950
Translation	MLLTLIILLPVVSKFSFVSLSAPQHWSCPEGTLAGNGNSTCVGPAPFLIFSHGNSIFRIDTEGTNYEQLVVDAGVSVIMDFHYNEKRIYWVDLERQLLQRVFLNGSRQERVCNIEKNVSGMAINWINEEVIWSNQQEGIITVTDMKGNNSHILLSALKYPANVAVDPVERFIFWSSEVAGSLYRADLDGVGVKALLETSEKITAVSLDVLDKRLFWIQYNREGSNSLICSCDYDGGSVHISKHPTQHNLFAMSLFGDRIFYSTWKMKTIWIANKHTGKDMVRINLHSSFVPLGELKVVHPLAQPKAEDDTWEPDVNECAFWNHGCTLGCKNTPGSYYCTCPVGFVLLPDGKRCHQLVSCPRNVSECSHDCVLTSEGPLCFCPEGSVLERDGKTCSGCSSPDNGGCSQLCVPLSPVSWECDCFPGYDLQLDEKSCAASGPQPFLLFANSQDIRHMHFDGTDYGTLLSQQMGMVYALDHDPVENKIYFAHTALKWIERANMDGSQRERLIEEGVDVPEGLAVDWIGRRFYWTDRGKSLIGRSDLNGKRSKIITKENISQPRGIAVHPMAKRLFWTDTGINPRIESSSLQGLGRLVIASSDLIWPSGITIDFLTDKLYWCDAKQSVIEMANLDGSKRRRLTQNDVGHPFAVAVFEDYVWFSDWAMPSVMRVNKRTGKDRVRLQGSMLKPSSLVVVHPLAKPGADPCLYQNGGCEHICKKRLGTAWCSCREGFMKASDGKTCLALDGHQLLAGGEVDLKNQVTPLDILSKTRVSEDNITESQHMLVAEIMVSDQDDCAPVGCSMYARCISEGEDATCQCLKGFAGDGKLCSDIDECEMGVPVCPPASSKCINTEGGYVCRCSEGYQGDGIHCLDIDECQLGEHSCGENASCTNTEGGYTCMCAGRLSEPGLICPDSTPPPHLREDDHHYSVRNSDSECPLSHDGYCLHDGVCMYIEALDKYACNCVVGYIGERCQYRDLKWWELRHAGHGQQQKVIVVAVCVVVLVMLLLLSLWGAHYYRTQKLLSKNPKNPYEESSRDVRSRRPADTEDGMSSCPQPWFVVIKEHQDLKNGGQPVAGEDGQAADGSMQPTSWRQEPQLCGMGTEQGCWIPVSSDKGSCPQVMERSFHMPSYGTQTLEGGVEKPHSLLSANPLWQQRALDPPHQMELTQ

Target ORF information:

RefSeq Version	NM_001178131.2
Organism	Homo sapiens(human)
Definition	Homo sapiens epidermal growth factor (EGF), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001178131.2

ORF Insert Sequence:

ATGCTGCTCA CTCTTATCAT TCTGTTGCCA GTAGTTTCAA AATTTAGTTT TGTTAGTCTC 
TCAGCACCGC AGCACTGGAG CTGTCCTGAA GGTACTCTCG CAGGAAATGG GAATTCTACT 
TGTGTGGGTC CTGCACCCTT CTTAATTTTC TCCCATGGAA ATAGTATCTT TAGGATTGAC 
ACAGAAGGAA CCAATTATGA GCAATTGGTG GTGGATGCTG GTGTCTCAGT GATCATGGAT 
TTTCATTATA ATGAGAAAAG AATCTATTGG GTGGATTTAG AAAGACAACT TTTGCAAAGA 
GTTTTTCTGA ATGGGTCAAG GCAAGAGAGA GTATGTAATA TAGAGAAAAA TGTTTCTGGA 
ATGGCAATAA ATTGGATAAA TGAAGAAGTT ATTTGGTCAA ATCAACAGGA AGGAATCATT 
ACAGTAACAG ATATGAAAGG AAATAATTCC CACATTCTTT TAAGTGCTTT AAAATATCCT 
GCAAATGTAG CAGTTGATCC AGTAGAAAGG TTTATATTTT GGTCTTCAGA GGTGGCTGGA 
AGCCTTTATA GAGCAGATCT CGATGGTGTG GGAGTGAAGG CTCTGTTGGA GACATCAGAG 
AAAATAACAG CTGTGTCATT GGATGTGCTT GATAAGCGGC TGTTTTGGAT TCAGTACAAC 
AGAGAAGGAA GCAATTCTCT TATTTGCTCC TGTGATTATG ATGGAGGTTC TGTCCACATT 
AGTAAACATC CAACACAGCA TAATTTGTTT GCAATGTCCC TTTTTGGTGA CCGTATCTTC 
TATTCAACAT GGAAAATGAA GACAATTTGG ATAGCCAACA AACACACTGG AAAGGACATG 
GTTAGAATTA ACCTCCATTC ATCATTTGTA CCACTTGGTG AACTGAAAGT AGTGCATCCA 
CTTGCACAAC CCAAGGCAGA AGATGACACT TGGGAGCCTG ATGTTAATGA ATGTGCTTTT 
TGGAATCATG GCTGTACTCT TGGGTGTAAA AACACCCCTG GATCCTATTA CTGCACGTGC 
CCTGTAGGAT TTGTTCTGCT TCCTGATGGG AAACGATGTC ATCAACTTGT TTCCTGTCCA 
CGCAATGTGT CTGAATGCAG CCATGACTGT GTTCTGACAT CAGAAGGTCC CTTATGTTTC 
TGTCCTGAAG GCTCAGTGCT TGAGAGAGAT GGGAAAACAT GTAGCGGTTG TTCCTCACCC 
GATAATGGTG GATGTAGCCA GCTCTGCGTT CCTCTTAGCC CAGTATCCTG GGAATGTGAT 
TGCTTTCCTG GGTATGACCT ACAACTGGAT GAAAAAAGCT GTGCAGCTTC AGGACCACAA 
CCATTTTTGC TGTTTGCCAA TTCTCAAGAT ATTCGACACA TGCATTTTGA TGGAACAGAC 
TATGGAACTC TGCTCAGCCA GCAGATGGGA ATGGTTTATG CCCTAGATCA TGACCCTGTG 
GAAAATAAGA TATACTTTGC CCATACAGCC CTGAAGTGGA TAGAGAGAGC TAATATGGAT 
GGTTCCCAGC GAGAAAGGCT TATTGAGGAA GGAGTAGATG TGCCAGAAGG TCTTGCTGTG 
GACTGGATTG GCCGTAGATT CTATTGGACA GACAGAGGGA AATCTCTGAT TGGAAGGAGT 
GATTTAAATG GGAAACGTTC CAAAATAATC ACTAAGGAGA ACATCTCTCA ACCACGAGGA 
ATTGCTGTTC ATCCAATGGC CAAGAGATTA TTCTGGACTG ATACAGGGAT TAATCCACGA 
ATTGAAAGTT CTTCCCTCCA AGGCCTTGGC CGTCTGGTTA TAGCCAGCTC TGATCTAATC 
TGGCCCAGTG GAATAACGAT TGACTTCTTA ACTGACAAGT TGTACTGGTG CGATGCCAAG 
CAGTCTGTGA TTGAAATGGC CAATCTGGAT GGTTCAAAAC GCCGAAGACT TACCCAGAAT 
GATGTAGGTC ACCCATTTGC TGTAGCAGTG TTTGAGGATT ATGTGTGGTT CTCAGATTGG 
GCTATGCCAT CAGTAATGAG AGTAAACAAG AGGACTGGCA AAGATAGAGT ACGTCTCCAA 
GGCAGCATGC TGAAGCCCTC ATCACTGGTT GTGGTTCATC CATTGGCAAA ACCAGGAGCA 
GATCCCTGCT TATATCAAAA CGGAGGCTGT GAACATATTT GCAAAAAGAG GCTTGGAACT 
GCTTGGTGTT CGTGTCGTGA AGGTTTTATG AAAGCCTCAG ATGGGAAAAC GTGTCTGGCT 
CTGGATGGTC ATCAGCTGTT GGCAGGTGGT GAAGTTGATC TAAAGAACCA AGTAACACCA 
TTGGACATCT TGTCCAAGAC TAGAGTGTCA GAAGATAACA TTACAGAATC TCAACACATG 
CTAGTGGCTG AAATCATGGT GTCAGATCAA GATGACTGTG CTCCTGTGGG ATGCAGCATG 
TATGCTCGGT GTATTTCAGA GGGAGAGGAT GCCACATGTC AGTGTTTGAA AGGATTTGCT 
GGGGATGGAA AACTATGTTC TGATATAGAT GAATGTGAGA TGGGTGTCCC AGTGTGCCCC 
CCTGCCTCCT CCAAGTGCAT CAACACCGAA GGTGGTTATG TCTGCCGGTG CTCAGAAGGC 
TACCAAGGAG ATGGGATTCA CTGTCTTGAT ATTGATGAGT GCCAACTGGG GGAGCACAGC 
TGTGGAGAGA ATGCCAGCTG CACAAATACA GAGGGAGGCT ATACCTGCAT GTGTGCTGGA 
CGCCTGTCTG AACCAGGACT GATTTGCCCT GACTCTACTC CACCCCCTCA CCTCAGGGAA 
GATGACCACC ACTATTCCGT AAGAAATAGT GACTCTGAAT GTCCCCTGTC CCACGATGGG 
TACTGCCTCC ATGATGGTGT GTGCATGTAT ATTGAAGCAT TGGACAAGTA TGCATGCAAC 
TGTGTTGTTG GCTACATCGG GGAGCGATGT CAGTACCGAG ACCTGAAGTG GTGGGAACTG 
CGCCACGCTG GCCACGGGCA GCAGCAGAAG GTCATCGTGG TGGCTGTCTG CGTGGTGGTG 
CTTGTCATGC TGCTCCTCCT GAGCCTGTGG GGGGCCCACT ACTACAGGAC TCAGAAGCTG 
CTATCGAAAA ACCCAAAGAA TCCTTATGAG GAGTCGAGCA GAGATGTGAG GAGTCGCAGG 
CCTGCTGACA CTGAGGATGG GATGTCCTCT TGCCCTCAAC CTTGGTTTGT GGTTATAAAA 
GAACACCAAG ACCTCAAGAA TGGGGGTCAA CCAGTGGCTG GTGAGGATGG CCAGGCAGCA 
GATGGGTCAA TGCAACCAAC TTCATGGAGG CAGGAGCCCC AGTTATGTGG AATGGGCACA 
GAGCAAGGCT GCTGGATTCC AGTATCCAGT GATAAGGGCT CCTGTCCCCA GGTAATGGAG 
CGAAGCTTTC ATATGCCCTC CTATGGGACA CAGACCCTTG AAGGGGGTGT CGAGAAGCCC 
CATTCTCTCC TATCAGCTAA CCCATTATGG CAACAAAGGG CCCTGGACCC ACCACACCAA 
ATGGAGCTGA CTCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EGF ( NM_001178130.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001171601.1 Homo sapiens epidermal growth factor (EGF), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu16028
Clone ID Related Accession (Same CDS sequence)	NM_001178130.2 , NM_001178130.3
Accession Version	NM_001178130.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3501bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-25
Organism	Homo sapiens(human)
Product	pro-epidermal growth factor isoform 2 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC005509.1, BC143357.1, AC004050.1 and CA437074.1. On Jan 8, 2016 this sequence version replaced NM_001178130.1. Transcript Variant: This variant (2) lacks an in-frame exon in the coding region, compared to variant 1. The encoded isoform (2) is shorter than isoform 1. This isoform (2) may undergo proteolytic processing similar to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC143357.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968832, SAMEA2145313 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCTGCTCA CTCTTATCAT TCTGTTGCCA GTAGTTTCAA AATTTAGTTT TGTTAGTCTC 
TCAGCACCGC AGCACTGGAG CTGTCCTGAA GGTACTCTCG CAGGAAATGG GAATTCTACT 
TGTGTGGGTC CTGCACCCTT CTTAATTTTC TCCCATGGAA ATAGTATCTT TAGGATTGAC 
ACAGAAGGAA CCAATTATGA GCAATTGGTG GTGGATGCTG GTGTCTCAGT GATCATGGAT 
TTTCATTATA ATGAGAAAAG AATCTATTGG GTGGATTTAG AAAGACAACT TTTGCAAAGA 
GTTTTTCTGA ATGGGTCAAG GCAAGAGAGA GTATGTAATA TAGAGAAAAA TGTTTCTGGA 
ATGGCAATAA ATTGGATAAA TGAAGAAGTT ATTTGGTCAA ATCAACAGGA AGGAATCATT 
ACAGTAACAG ATATGAAAGG AAATAATTCC CACATTCTTT TAAGTGCTTT AAAATATCCT 
GCAAATGTAG CAGTTGATCC AGTAGAAAGG TTTATATTTT GGTCTTCAGA GGTGGCTGGA 
AGCCTTTATA GAGCAGATCT CGATGGTGTG GGAGTGAAGG CTCTGTTGGA GACATCAGAG 
AAAATAACAG CTGTGTCATT GGATGTGCTT GATAAGCGGC TGTTTTGGAT TCAGTACAAC 
AGAGAAGGAA GCAATTCTCT TATTTGCTCC TGTGATTATG ATGGAGGTTC TGTCCACATT 
AGTAAACATC CAACACAGCA TAATTTGTTT GCAATGTCCC TTTTTGGTGA CCGTATCTTC 
TATTCAACAT GGAAAATGAA GACAATTTGG ATAGCCAACA AACACACTGG AAAGGACATG 
GTTAGAATTA ACCTCCATTC ATCATTTGTA CCACTTGGTG AACTGAAAGT AGTGCATCCA 
CTTGCACAAC CCAAGGCAGA AGATGACACT TGGGAGCCTG AGCAGAAACT TTGCAAATTG 
AGGAAAGGAA ACTGCAGCAG CACTGTGTGT GGGCAAGACC TCCAGTCACA CTTGTGCATG 
TGTGCAGAGG GATACGCCCT AAGTCGAGAC CGGAAGTACT GTGAAGATGT TAATGAATGT 
GCTTTTTGGA ATCATGGCTG TACTCTTGGG TGTAAAAACA CCCCTGGATC CTATTACTGC 
ACGTGCCCTG TAGGATTTGT TCTGCTTCCT GATGGGAAAC GATGTCATCA ACTTGTTTCC 
TGTCCACGCA ATGTGTCTGA ATGCAGCCAT GACTGTGTTC TGACATCAGA AGGTCCCTTA 
TGTTTCTGTC CTGAAGGCTC AGTGCTTGAG AGAGATGGGA AAACATGTAG CGGTTGTTCC 
TCACCCGATA ATGGTGGATG TAGCCAGCTC TGCGTTCCTC TTAGCCCAGT ATCCTGGGAA 
TGTGATTGCT TTCCTGGGTA TGACCTACAA CTGGATGAAA AAAGCTGTGC AGCTTCAGGA 
CCACAACCAT TTTTGCTGTT TGCCAATTCT CAAGATATTC GACACATGCA TTTTGATGGA 
ACAGACTATG GAACTCTGCT CAGCCAGCAG ATGGGAATGG TTTATGCCCT AGATCATGAC 
CCTGTGGAAA ATAAGATATA CTTTGCCCAT ACAGCCCTGA AGTGGATAGA GAGAGCTAAT 
ATGGATGGTT CCCAGCGAGA AAGGCTTATT GAGGAAGGAG TAGATGTGCC AGAAGGTCTT 
GCTGTGGACT GGATTGGCCG TAGATTCTAT TGGACAGACA GAGGGAAATC TCTGATTGGA 
AGGAGTGATT TAAATGGGAA ACGTTCCAAA ATAATCACTA AGGAGAACAT CTCTCAACCA 
CGAGGAATTG CTGTTCATCC AATGGCCAAG AGATTATTCT GGACTGATAC AGGGATTAAT 
CCACGAATTG AAAGTTCTTC CCTCCAAGGC CTTGGCCGTC TGGTTATAGC CAGCTCTGAT 
CTAATCTGGC CCAGTGGAAT AACGATTGAC TTCTTAACTG ACAAGTTGTA CTGGTGCGAT 
GCCAAGCAGT CTGTGATTGA AATGGCCAAT CTGGATGGTT CAAAACGCCG AAGACTTACC 
CAGAATGATG TAGGTCACCC ATTTGCTGTA GCAGTGTTTG AGGATTATGT GTGGTTCTCA 
GATTGGGCTA TGCCATCAGT AATGAGAGTA AACAAGAGGA CTGGCAAAGA TAGAGTACGT 
CTCCAAGGCA GCATGCTGAA GCCCTCATCA CTGGTTGTGG TTCATCCATT GGCAAAACCA 
GGAGCAGATC CCTGCTTATA TCAAAACGGA GGCTGTGAAC ATATTTGCAA AAAGAGGCTT 
GGAACTGCTT GGTGTTCGTG TCGTGAAGGT TTTATGAAAG CCTCAGATGG GAAAACGTGT 
CTGGCTCTGG ATGGTCATCA GCTGTTGGCA GGTGGTGAAG TTGATCTAAA GAACCAAGTA 
ACACCATTGG ACATCTTGTC CAAGACTAGA GTGTCAGAAG ATAACATTAC AGAATCTCAA 
CACATGCTAG TGGCTGAAAT CATGGTGTCA GATCAAGATG ACTGTGCTCC TGTGGGATGC 
AGCATGTATG CTCGGTGTAT TTCAGAGGGA GAGGATGCCA CATGTCAGTG TTTGAAAGGA 
TTTGCTGGGG ATGGAAAACT ATGTTCTGAT ATAGATGAAT GTGAGATGGG TGTCCCAGTG 
TGCCCCCCTG CCTCCTCCAA GTGCATCAAC ACCGAAGGTG GTTATGTCTG CCGGTGCTCA 
GAAGGCTACC AAGGAGATGG GATTCACTGT CTTGACTCTA CTCCACCCCC TCACCTCAGG 
GAAGATGACC ACCACTATTC CGTAAGAAAT AGTGACTCTG AATGTCCCCT GTCCCACGAT 
GGGTACTGCC TCCATGATGG TGTGTGCATG TATATTGAAG CATTGGACAA GTATGCATGC 
AACTGTGTTG TTGGCTACAT CGGGGAGCGA TGTCAGTACC GAGACCTGAA GTGGTGGGAA 
CTGCGCCACG CTGGCCACGG GCAGCAGCAG AAGGTCATCG TGGTGGCTGT CTGCGTGGTG 
GTGCTTGTCA TGCTGCTCCT CCTGAGCCTG TGGGGGGCCC ACTACTACAG GACTCAGAAG 
CTGCTATCGA AAAACCCAAA GAATCCTTAT GAGGAGTCGA GCAGAGATGT GAGGAGTCGC 
AGGCCTGCTG ACACTGAGGA TGGGATGTCC TCTTGCCCTC AACCTTGGTT TGTGGTTATA 
AAAGAACACC AAGACCTCAA GAATGGGGGT CAACCAGTGG CTGGTGAGGA TGGCCAGGCA 
GCAGATGGGT CAATGCAACC AACTTCATGG AGGCAGGAGC CCCAGTTATG TGGAATGGGC 
ACAGAGCAAG GCTGCTGGAT TCCAGTATCC AGTGATAAGG GCTCCTGTCC CCAGGTAATG 
GAGCGAAGCT TTCATATGCC CTCCTATGGG ACACAGACCC TTGAAGGGGG TGTCGAGAAG 
CCCCATTCTC TCCTATCAGC TAACCCATTA TGGCAACAAA GGGCCCTGGA CCCACCACAC 
CAAATGGAGC TGACTCAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001171601.1
CDS	453..3953
Translation	MLLTLIILLPVVSKFSFVSLSAPQHWSCPEGTLAGNGNSTCVGPAPFLIFSHGNSIFRIDTEGTNYEQLVVDAGVSVIMDFHYNEKRIYWVDLERQLLQRVFLNGSRQERVCNIEKNVSGMAINWINEEVIWSNQQEGIITVTDMKGNNSHILLSALKYPANVAVDPVERFIFWSSEVAGSLYRADLDGVGVKALLETSEKITAVSLDVLDKRLFWIQYNREGSNSLICSCDYDGGSVHISKHPTQHNLFAMSLFGDRIFYSTWKMKTIWIANKHTGKDMVRINLHSSFVPLGELKVVHPLAQPKAEDDTWEPEQKLCKLRKGNCSSTVCGQDLQSHLCMCAEGYALSRDRKYCEDVNECAFWNHGCTLGCKNTPGSYYCTCPVGFVLLPDGKRCHQLVSCPRNVSECSHDCVLTSEGPLCFCPEGSVLERDGKTCSGCSSPDNGGCSQLCVPLSPVSWECDCFPGYDLQLDEKSCAASGPQPFLLFANSQDIRHMHFDGTDYGTLLSQQMGMVYALDHDPVENKIYFAHTALKWIERANMDGSQRERLIEEGVDVPEGLAVDWIGRRFYWTDRGKSLIGRSDLNGKRSKIITKENISQPRGIAVHPMAKRLFWTDTGINPRIESSSLQGLGRLVIASSDLIWPSGITIDFLTDKLYWCDAKQSVIEMANLDGSKRRRLTQNDVGHPFAVAVFEDYVWFSDWAMPSVMRVNKRTGKDRVRLQGSMLKPSSLVVVHPLAKPGADPCLYQNGGCEHICKKRLGTAWCSCREGFMKASDGKTCLALDGHQLLAGGEVDLKNQVTPLDILSKTRVSEDNITESQHMLVAEIMVSDQDDCAPVGCSMYARCISEGEDATCQCLKGFAGDGKLCSDIDECEMGVPVCPPASSKCINTEGGYVCRCSEGYQGDGIHCLDSTPPPHLREDDHHYSVRNSDSECPLSHDGYCLHDGVCMYIEALDKYACNCVVGYIGERCQYRDLKWWELRHAGHGQQQKVIVVAVCVVVLVMLLLLSLWGAHYYRTQKLLSKNPKNPYEESSRDVRSRRPADTEDGMSSCPQPWFVVIKEHQDLKNGGQPVAGEDGQAADGSMQPTSWRQEPQLCGMGTEQGCWIPVSSDKGSCPQVMERSFHMPSYGTQTLEGGVEKPHSLLSANPLWQQRALDPPHQMELTQ

Target ORF information:

RefSeq Version	NM_001178130.2
Organism	Homo sapiens(human)
Definition	Homo sapiens epidermal growth factor (EGF), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001178130.2

ORF Insert Sequence:

ATGCTGCTCA CTCTTATCAT TCTGTTGCCA GTAGTTTCAA AATTTAGTTT TGTTAGTCTC 
TCAGCACCGC AGCACTGGAG CTGTCCTGAA GGTACTCTCG CAGGAAATGG GAATTCTACT 
TGTGTGGGTC CTGCACCCTT CTTAATTTTC TCCCATGGAA ATAGTATCTT TAGGATTGAC 
ACAGAAGGAA CCAATTATGA GCAATTGGTG GTGGATGCTG GTGTCTCAGT GATCATGGAT 
TTTCATTATA ATGAGAAAAG AATCTATTGG GTGGATTTAG AAAGACAACT TTTGCAAAGA 
GTTTTTCTGA ATGGGTCAAG GCAAGAGAGA GTATGTAATA TAGAGAAAAA TGTTTCTGGA 
ATGGCAATAA ATTGGATAAA TGAAGAAGTT ATTTGGTCAA ATCAACAGGA AGGAATCATT 
ACAGTAACAG ATATGAAAGG AAATAATTCC CACATTCTTT TAAGTGCTTT AAAATATCCT 
GCAAATGTAG CAGTTGATCC AGTAGAAAGG TTTATATTTT GGTCTTCAGA GGTGGCTGGA 
AGCCTTTATA GAGCAGATCT CGATGGTGTG GGAGTGAAGG CTCTGTTGGA GACATCAGAG 
AAAATAACAG CTGTGTCATT GGATGTGCTT GATAAGCGGC TGTTTTGGAT TCAGTACAAC 
AGAGAAGGAA GCAATTCTCT TATTTGCTCC TGTGATTATG ATGGAGGTTC TGTCCACATT 
AGTAAACATC CAACACAGCA TAATTTGTTT GCAATGTCCC TTTTTGGTGA CCGTATCTTC 
TATTCAACAT GGAAAATGAA GACAATTTGG ATAGCCAACA AACACACTGG AAAGGACATG 
GTTAGAATTA ACCTCCATTC ATCATTTGTA CCACTTGGTG AACTGAAAGT AGTGCATCCA 
CTTGCACAAC CCAAGGCAGA AGATGACACT TGGGAGCCTG AGCAGAAACT TTGCAAATTG 
AGGAAAGGAA ACTGCAGCAG CACTGTGTGT GGGCAAGACC TCCAGTCACA CTTGTGCATG 
TGTGCAGAGG GATACGCCCT AAGTCGAGAC CGGAAGTACT GTGAAGATGT TAATGAATGT 
GCTTTTTGGA ATCATGGCTG TACTCTTGGG TGTAAAAACA CCCCTGGATC CTATTACTGC 
ACGTGCCCTG TAGGATTTGT TCTGCTTCCT GATGGGAAAC GATGTCATCA ACTTGTTTCC 
TGTCCACGCA ATGTGTCTGA ATGCAGCCAT GACTGTGTTC TGACATCAGA AGGTCCCTTA 
TGTTTCTGTC CTGAAGGCTC AGTGCTTGAG AGAGATGGGA AAACATGTAG CGGTTGTTCC 
TCACCCGATA ATGGTGGATG TAGCCAGCTC TGCGTTCCTC TTAGCCCAGT ATCCTGGGAA 
TGTGATTGCT TTCCTGGGTA TGACCTACAA CTGGATGAAA AAAGCTGTGC AGCTTCAGGA 
CCACAACCAT TTTTGCTGTT TGCCAATTCT CAAGATATTC GACACATGCA TTTTGATGGA 
ACAGACTATG GAACTCTGCT CAGCCAGCAG ATGGGAATGG TTTATGCCCT AGATCATGAC 
CCTGTGGAAA ATAAGATATA CTTTGCCCAT ACAGCCCTGA AGTGGATAGA GAGAGCTAAT 
ATGGATGGTT CCCAGCGAGA AAGGCTTATT GAGGAAGGAG TAGATGTGCC AGAAGGTCTT 
GCTGTGGACT GGATTGGCCG TAGATTCTAT TGGACAGACA GAGGGAAATC TCTGATTGGA 
AGGAGTGATT TAAATGGGAA ACGTTCCAAA ATAATCACTA AGGAGAACAT CTCTCAACCA 
CGAGGAATTG CTGTTCATCC AATGGCCAAG AGATTATTCT GGACTGATAC AGGGATTAAT 
CCACGAATTG AAAGTTCTTC CCTCCAAGGC CTTGGCCGTC TGGTTATAGC CAGCTCTGAT 
CTAATCTGGC CCAGTGGAAT AACGATTGAC TTCTTAACTG ACAAGTTGTA CTGGTGCGAT 
GCCAAGCAGT CTGTGATTGA AATGGCCAAT CTGGATGGTT CAAAACGCCG AAGACTTACC 
CAGAATGATG TAGGTCACCC ATTTGCTGTA GCAGTGTTTG AGGATTATGT GTGGTTCTCA 
GATTGGGCTA TGCCATCAGT AATGAGAGTA AACAAGAGGA CTGGCAAAGA TAGAGTACGT 
CTCCAAGGCA GCATGCTGAA GCCCTCATCA CTGGTTGTGG TTCATCCATT GGCAAAACCA 
GGAGCAGATC CCTGCTTATA TCAAAACGGA GGCTGTGAAC ATATTTGCAA AAAGAGGCTT 
GGAACTGCTT GGTGTTCGTG TCGTGAAGGT TTTATGAAAG CCTCAGATGG GAAAACGTGT 
CTGGCTCTGG ATGGTCATCA GCTGTTGGCA GGTGGTGAAG TTGATCTAAA GAACCAAGTA 
ACACCATTGG ACATCTTGTC CAAGACTAGA GTGTCAGAAG ATAACATTAC AGAATCTCAA 
CACATGCTAG TGGCTGAAAT CATGGTGTCA GATCAAGATG ACTGTGCTCC TGTGGGATGC 
AGCATGTATG CTCGGTGTAT TTCAGAGGGA GAGGATGCCA CATGTCAGTG TTTGAAAGGA 
TTTGCTGGGG ATGGAAAACT ATGTTCTGAT ATAGATGAAT GTGAGATGGG TGTCCCAGTG 
TGCCCCCCTG CCTCCTCCAA GTGCATCAAC ACCGAAGGTG GTTATGTCTG CCGGTGCTCA 
GAAGGCTACC AAGGAGATGG GATTCACTGT CTTGACTCTA CTCCACCCCC TCACCTCAGG 
GAAGATGACC ACCACTATTC CGTAAGAAAT AGTGACTCTG AATGTCCCCT GTCCCACGAT 
GGGTACTGCC TCCATGATGG TGTGTGCATG TATATTGAAG CATTGGACAA GTATGCATGC 
AACTGTGTTG TTGGCTACAT CGGGGAGCGA TGTCAGTACC GAGACCTGAA GTGGTGGGAA 
CTGCGCCACG CTGGCCACGG GCAGCAGCAG AAGGTCATCG TGGTGGCTGT CTGCGTGGTG 
GTGCTTGTCA TGCTGCTCCT CCTGAGCCTG TGGGGGGCCC ACTACTACAG GACTCAGAAG 
CTGCTATCGA AAAACCCAAA GAATCCTTAT GAGGAGTCGA GCAGAGATGT GAGGAGTCGC 
AGGCCTGCTG ACACTGAGGA TGGGATGTCC TCTTGCCCTC AACCTTGGTT TGTGGTTATA 
AAAGAACACC AAGACCTCAA GAATGGGGGT CAACCAGTGG CTGGTGAGGA TGGCCAGGCA 
GCAGATGGGT CAATGCAACC AACTTCATGG AGGCAGGAGC CCCAGTTATG TGGAATGGGC 
ACAGAGCAAG GCTGCTGGAT TCCAGTATCC AGTGATAAGG GCTCCTGTCC CCAGGTAATG 
GAGCGAAGCT TTCATATGCC CTCCTATGGG ACACAGACCC TTGAAGGGGG TGTCGAGAAG 
CCCCATTCTC TCCTATCAGC TAACCCATTA TGGCAACAAA GGGCCCTGGA CCCACCACAC 
CAAATGGAGC TGACTCAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EGF ( NM_001178130.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001171601.1 Homo sapiens epidermal growth factor (EGF), transcript variant 2, mRNA.

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CloneID	OHu16028
Clone ID Related Accession (Same CDS sequence)	NM_001178130.2 , NM_001178130.3
Accession Version	NM_001178130.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3501bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-03
Organism	Homo sapiens(human)
Product	pro-epidermal growth factor isoform 2 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC005509.1, BC143357.1, AC004050.1 and CA437074.1. On May 31, 2019 this sequence version replaced NM_001178130.2. Transcript Variant: This variant (2) lacks an in-frame exon in the coding region, compared to variant 1. The encoded isoform (2) is shorter than isoform 1. This isoform (2) may undergo proteolytic processing similar to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC143357.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968832, SAMEA2145313 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCTGCTCA CTCTTATCAT TCTGTTGCCA GTAGTTTCAA AATTTAGTTT TGTTAGTCTC 
TCAGCACCGC AGCACTGGAG CTGTCCTGAA GGTACTCTCG CAGGAAATGG GAATTCTACT 
TGTGTGGGTC CTGCACCCTT CTTAATTTTC TCCCATGGAA ATAGTATCTT TAGGATTGAC 
ACAGAAGGAA CCAATTATGA GCAATTGGTG GTGGATGCTG GTGTCTCAGT GATCATGGAT 
TTTCATTATA ATGAGAAAAG AATCTATTGG GTGGATTTAG AAAGACAACT TTTGCAAAGA 
GTTTTTCTGA ATGGGTCAAG GCAAGAGAGA GTATGTAATA TAGAGAAAAA TGTTTCTGGA 
ATGGCAATAA ATTGGATAAA TGAAGAAGTT ATTTGGTCAA ATCAACAGGA AGGAATCATT 
ACAGTAACAG ATATGAAAGG AAATAATTCC CACATTCTTT TAAGTGCTTT AAAATATCCT 
GCAAATGTAG CAGTTGATCC AGTAGAAAGG TTTATATTTT GGTCTTCAGA GGTGGCTGGA 
AGCCTTTATA GAGCAGATCT CGATGGTGTG GGAGTGAAGG CTCTGTTGGA GACATCAGAG 
AAAATAACAG CTGTGTCATT GGATGTGCTT GATAAGCGGC TGTTTTGGAT TCAGTACAAC 
AGAGAAGGAA GCAATTCTCT TATTTGCTCC TGTGATTATG ATGGAGGTTC TGTCCACATT 
AGTAAACATC CAACACAGCA TAATTTGTTT GCAATGTCCC TTTTTGGTGA CCGTATCTTC 
TATTCAACAT GGAAAATGAA GACAATTTGG ATAGCCAACA AACACACTGG AAAGGACATG 
GTTAGAATTA ACCTCCATTC ATCATTTGTA CCACTTGGTG AACTGAAAGT AGTGCATCCA 
CTTGCACAAC CCAAGGCAGA AGATGACACT TGGGAGCCTG AGCAGAAACT TTGCAAATTG 
AGGAAAGGAA ACTGCAGCAG CACTGTGTGT GGGCAAGACC TCCAGTCACA CTTGTGCATG 
TGTGCAGAGG GATACGCCCT AAGTCGAGAC CGGAAGTACT GTGAAGATGT TAATGAATGT 
GCTTTTTGGA ATCATGGCTG TACTCTTGGG TGTAAAAACA CCCCTGGATC CTATTACTGC 
ACGTGCCCTG TAGGATTTGT TCTGCTTCCT GATGGGAAAC GATGTCATCA ACTTGTTTCC 
TGTCCACGCA ATGTGTCTGA ATGCAGCCAT GACTGTGTTC TGACATCAGA AGGTCCCTTA 
TGTTTCTGTC CTGAAGGCTC AGTGCTTGAG AGAGATGGGA AAACATGTAG CGGTTGTTCC 
TCACCCGATA ATGGTGGATG TAGCCAGCTC TGCGTTCCTC TTAGCCCAGT ATCCTGGGAA 
TGTGATTGCT TTCCTGGGTA TGACCTACAA CTGGATGAAA AAAGCTGTGC AGCTTCAGGA 
CCACAACCAT TTTTGCTGTT TGCCAATTCT CAAGATATTC GACACATGCA TTTTGATGGA 
ACAGACTATG GAACTCTGCT CAGCCAGCAG ATGGGAATGG TTTATGCCCT AGATCATGAC 
CCTGTGGAAA ATAAGATATA CTTTGCCCAT ACAGCCCTGA AGTGGATAGA GAGAGCTAAT 
ATGGATGGTT CCCAGCGAGA AAGGCTTATT GAGGAAGGAG TAGATGTGCC AGAAGGTCTT 
GCTGTGGACT GGATTGGCCG TAGATTCTAT TGGACAGACA GAGGGAAATC TCTGATTGGA 
AGGAGTGATT TAAATGGGAA ACGTTCCAAA ATAATCACTA AGGAGAACAT CTCTCAACCA 
CGAGGAATTG CTGTTCATCC AATGGCCAAG AGATTATTCT GGACTGATAC AGGGATTAAT 
CCACGAATTG AAAGTTCTTC CCTCCAAGGC CTTGGCCGTC TGGTTATAGC CAGCTCTGAT 
CTAATCTGGC CCAGTGGAAT AACGATTGAC TTCTTAACTG ACAAGTTGTA CTGGTGCGAT 
GCCAAGCAGT CTGTGATTGA AATGGCCAAT CTGGATGGTT CAAAACGCCG AAGACTTACC 
CAGAATGATG TAGGTCACCC ATTTGCTGTA GCAGTGTTTG AGGATTATGT GTGGTTCTCA 
GATTGGGCTA TGCCATCAGT AATGAGAGTA AACAAGAGGA CTGGCAAAGA TAGAGTACGT 
CTCCAAGGCA GCATGCTGAA GCCCTCATCA CTGGTTGTGG TTCATCCATT GGCAAAACCA 
GGAGCAGATC CCTGCTTATA TCAAAACGGA GGCTGTGAAC ATATTTGCAA AAAGAGGCTT 
GGAACTGCTT GGTGTTCGTG TCGTGAAGGT TTTATGAAAG CCTCAGATGG GAAAACGTGT 
CTGGCTCTGG ATGGTCATCA GCTGTTGGCA GGTGGTGAAG TTGATCTAAA GAACCAAGTA 
ACACCATTGG ACATCTTGTC CAAGACTAGA GTGTCAGAAG ATAACATTAC AGAATCTCAA 
CACATGCTAG TGGCTGAAAT CATGGTGTCA GATCAAGATG ACTGTGCTCC TGTGGGATGC 
AGCATGTATG CTCGGTGTAT TTCAGAGGGA GAGGATGCCA CATGTCAGTG TTTGAAAGGA 
TTTGCTGGGG ATGGAAAACT ATGTTCTGAT ATAGATGAAT GTGAGATGGG TGTCCCAGTG 
TGCCCCCCTG CCTCCTCCAA GTGCATCAAC ACCGAAGGTG GTTATGTCTG CCGGTGCTCA 
GAAGGCTACC AAGGAGATGG GATTCACTGT CTTGACTCTA CTCCACCCCC TCACCTCAGG 
GAAGATGACC ACCACTATTC CGTAAGAAAT AGTGACTCTG AATGTCCCCT GTCCCACGAT 
GGGTACTGCC TCCATGATGG TGTGTGCATG TATATTGAAG CATTGGACAA GTATGCATGC 
AACTGTGTTG TTGGCTACAT CGGGGAGCGA TGTCAGTACC GAGACCTGAA GTGGTGGGAA 
CTGCGCCACG CTGGCCACGG GCAGCAGCAG AAGGTCATCG TGGTGGCTGT CTGCGTGGTG 
GTGCTTGTCA TGCTGCTCCT CCTGAGCCTG TGGGGGGCCC ACTACTACAG GACTCAGAAG 
CTGCTATCGA AAAACCCAAA GAATCCTTAT GAGGAGTCGA GCAGAGATGT GAGGAGTCGC 
AGGCCTGCTG ACACTGAGGA TGGGATGTCC TCTTGCCCTC AACCTTGGTT TGTGGTTATA 
AAAGAACACC AAGACCTCAA GAATGGGGGT CAACCAGTGG CTGGTGAGGA TGGCCAGGCA 
GCAGATGGGT CAATGCAACC AACTTCATGG AGGCAGGAGC CCCAGTTATG TGGAATGGGC 
ACAGAGCAAG GCTGCTGGAT TCCAGTATCC AGTGATAAGG GCTCCTGTCC CCAGGTAATG 
GAGCGAAGCT TTCATATGCC CTCCTATGGG ACACAGACCC TTGAAGGGGG TGTCGAGAAG 
CCCCATTCTC TCCTATCAGC TAACCCATTA TGGCAACAAA GGGCCCTGGA CCCACCACAC 
CAAATGGAGC TGACTCAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001171601.1
CDS	454..3954
Translation	MLLTLIILLPVVSKFSFVSLSAPQHWSCPEGTLAGNGNSTCVGPAPFLIFSHGNSIFRIDTEGTNYEQLVVDAGVSVIMDFHYNEKRIYWVDLERQLLQRVFLNGSRQERVCNIEKNVSGMAINWINEEVIWSNQQEGIITVTDMKGNNSHILLSALKYPANVAVDPVERFIFWSSEVAGSLYRADLDGVGVKALLETSEKITAVSLDVLDKRLFWIQYNREGSNSLICSCDYDGGSVHISKHPTQHNLFAMSLFGDRIFYSTWKMKTIWIANKHTGKDMVRINLHSSFVPLGELKVVHPLAQPKAEDDTWEPEQKLCKLRKGNCSSTVCGQDLQSHLCMCAEGYALSRDRKYCEDVNECAFWNHGCTLGCKNTPGSYYCTCPVGFVLLPDGKRCHQLVSCPRNVSECSHDCVLTSEGPLCFCPEGSVLERDGKTCSGCSSPDNGGCSQLCVPLSPVSWECDCFPGYDLQLDEKSCAASGPQPFLLFANSQDIRHMHFDGTDYGTLLSQQMGMVYALDHDPVENKIYFAHTALKWIERANMDGSQRERLIEEGVDVPEGLAVDWIGRRFYWTDRGKSLIGRSDLNGKRSKIITKENISQPRGIAVHPMAKRLFWTDTGINPRIESSSLQGLGRLVIASSDLIWPSGITIDFLTDKLYWCDAKQSVIEMANLDGSKRRRLTQNDVGHPFAVAVFEDYVWFSDWAMPSVMRVNKRTGKDRVRLQGSMLKPSSLVVVHPLAKPGADPCLYQNGGCEHICKKRLGTAWCSCREGFMKASDGKTCLALDGHQLLAGGEVDLKNQVTPLDILSKTRVSEDNITESQHMLVAEIMVSDQDDCAPVGCSMYARCISEGEDATCQCLKGFAGDGKLCSDIDECEMGVPVCPPASSKCINTEGGYVCRCSEGYQGDGIHCLDSTPPPHLREDDHHYSVRNSDSECPLSHDGYCLHDGVCMYIEALDKYACNCVVGYIGERCQYRDLKWWELRHAGHGQQQKVIVVAVCVVVLVMLLLLSLWGAHYYRTQKLLSKNPKNPYEESSRDVRSRRPADTEDGMSSCPQPWFVVIKEHQDLKNGGQPVAGEDGQAADGSMQPTSWRQEPQLCGMGTEQGCWIPVSSDKGSCPQVMERSFHMPSYGTQTLEGGVEKPHSLLSANPLWQQRALDPPHQMELTQ

Target ORF information:

RefSeq Version	NM_001178130.3
Organism	Homo sapiens(human)
Definition	Homo sapiens epidermal growth factor (EGF), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001178130.3

ORF Insert Sequence:

ATGCTGCTCA CTCTTATCAT TCTGTTGCCA GTAGTTTCAA AATTTAGTTT TGTTAGTCTC 
TCAGCACCGC AGCACTGGAG CTGTCCTGAA GGTACTCTCG CAGGAAATGG GAATTCTACT 
TGTGTGGGTC CTGCACCCTT CTTAATTTTC TCCCATGGAA ATAGTATCTT TAGGATTGAC 
ACAGAAGGAA CCAATTATGA GCAATTGGTG GTGGATGCTG GTGTCTCAGT GATCATGGAT 
TTTCATTATA ATGAGAAAAG AATCTATTGG GTGGATTTAG AAAGACAACT TTTGCAAAGA 
GTTTTTCTGA ATGGGTCAAG GCAAGAGAGA GTATGTAATA TAGAGAAAAA TGTTTCTGGA 
ATGGCAATAA ATTGGATAAA TGAAGAAGTT ATTTGGTCAA ATCAACAGGA AGGAATCATT 
ACAGTAACAG ATATGAAAGG AAATAATTCC CACATTCTTT TAAGTGCTTT AAAATATCCT 
GCAAATGTAG CAGTTGATCC AGTAGAAAGG TTTATATTTT GGTCTTCAGA GGTGGCTGGA 
AGCCTTTATA GAGCAGATCT CGATGGTGTG GGAGTGAAGG CTCTGTTGGA GACATCAGAG 
AAAATAACAG CTGTGTCATT GGATGTGCTT GATAAGCGGC TGTTTTGGAT TCAGTACAAC 
AGAGAAGGAA GCAATTCTCT TATTTGCTCC TGTGATTATG ATGGAGGTTC TGTCCACATT 
AGTAAACATC CAACACAGCA TAATTTGTTT GCAATGTCCC TTTTTGGTGA CCGTATCTTC 
TATTCAACAT GGAAAATGAA GACAATTTGG ATAGCCAACA AACACACTGG AAAGGACATG 
GTTAGAATTA ACCTCCATTC ATCATTTGTA CCACTTGGTG AACTGAAAGT AGTGCATCCA 
CTTGCACAAC CCAAGGCAGA AGATGACACT TGGGAGCCTG AGCAGAAACT TTGCAAATTG 
AGGAAAGGAA ACTGCAGCAG CACTGTGTGT GGGCAAGACC TCCAGTCACA CTTGTGCATG 
TGTGCAGAGG GATACGCCCT AAGTCGAGAC CGGAAGTACT GTGAAGATGT TAATGAATGT 
GCTTTTTGGA ATCATGGCTG TACTCTTGGG TGTAAAAACA CCCCTGGATC CTATTACTGC 
ACGTGCCCTG TAGGATTTGT TCTGCTTCCT GATGGGAAAC GATGTCATCA ACTTGTTTCC 
TGTCCACGCA ATGTGTCTGA ATGCAGCCAT GACTGTGTTC TGACATCAGA AGGTCCCTTA 
TGTTTCTGTC CTGAAGGCTC AGTGCTTGAG AGAGATGGGA AAACATGTAG CGGTTGTTCC 
TCACCCGATA ATGGTGGATG TAGCCAGCTC TGCGTTCCTC TTAGCCCAGT ATCCTGGGAA 
TGTGATTGCT TTCCTGGGTA TGACCTACAA CTGGATGAAA AAAGCTGTGC AGCTTCAGGA 
CCACAACCAT TTTTGCTGTT TGCCAATTCT CAAGATATTC GACACATGCA TTTTGATGGA 
ACAGACTATG GAACTCTGCT CAGCCAGCAG ATGGGAATGG TTTATGCCCT AGATCATGAC 
CCTGTGGAAA ATAAGATATA CTTTGCCCAT ACAGCCCTGA AGTGGATAGA GAGAGCTAAT 
ATGGATGGTT CCCAGCGAGA AAGGCTTATT GAGGAAGGAG TAGATGTGCC AGAAGGTCTT 
GCTGTGGACT GGATTGGCCG TAGATTCTAT TGGACAGACA GAGGGAAATC TCTGATTGGA 
AGGAGTGATT TAAATGGGAA ACGTTCCAAA ATAATCACTA AGGAGAACAT CTCTCAACCA 
CGAGGAATTG CTGTTCATCC AATGGCCAAG AGATTATTCT GGACTGATAC AGGGATTAAT 
CCACGAATTG AAAGTTCTTC CCTCCAAGGC CTTGGCCGTC TGGTTATAGC CAGCTCTGAT 
CTAATCTGGC CCAGTGGAAT AACGATTGAC TTCTTAACTG ACAAGTTGTA CTGGTGCGAT 
GCCAAGCAGT CTGTGATTGA AATGGCCAAT CTGGATGGTT CAAAACGCCG AAGACTTACC 
CAGAATGATG TAGGTCACCC ATTTGCTGTA GCAGTGTTTG AGGATTATGT GTGGTTCTCA 
GATTGGGCTA TGCCATCAGT AATGAGAGTA AACAAGAGGA CTGGCAAAGA TAGAGTACGT 
CTCCAAGGCA GCATGCTGAA GCCCTCATCA CTGGTTGTGG TTCATCCATT GGCAAAACCA 
GGAGCAGATC CCTGCTTATA TCAAAACGGA GGCTGTGAAC ATATTTGCAA AAAGAGGCTT 
GGAACTGCTT GGTGTTCGTG TCGTGAAGGT TTTATGAAAG CCTCAGATGG GAAAACGTGT 
CTGGCTCTGG ATGGTCATCA GCTGTTGGCA GGTGGTGAAG TTGATCTAAA GAACCAAGTA 
ACACCATTGG ACATCTTGTC CAAGACTAGA GTGTCAGAAG ATAACATTAC AGAATCTCAA 
CACATGCTAG TGGCTGAAAT CATGGTGTCA GATCAAGATG ACTGTGCTCC TGTGGGATGC 
AGCATGTATG CTCGGTGTAT TTCAGAGGGA GAGGATGCCA CATGTCAGTG TTTGAAAGGA 
TTTGCTGGGG ATGGAAAACT ATGTTCTGAT ATAGATGAAT GTGAGATGGG TGTCCCAGTG 
TGCCCCCCTG CCTCCTCCAA GTGCATCAAC ACCGAAGGTG GTTATGTCTG CCGGTGCTCA 
GAAGGCTACC AAGGAGATGG GATTCACTGT CTTGACTCTA CTCCACCCCC TCACCTCAGG 
GAAGATGACC ACCACTATTC CGTAAGAAAT AGTGACTCTG AATGTCCCCT GTCCCACGAT 
GGGTACTGCC TCCATGATGG TGTGTGCATG TATATTGAAG CATTGGACAA GTATGCATGC 
AACTGTGTTG TTGGCTACAT CGGGGAGCGA TGTCAGTACC GAGACCTGAA GTGGTGGGAA 
CTGCGCCACG CTGGCCACGG GCAGCAGCAG AAGGTCATCG TGGTGGCTGT CTGCGTGGTG 
GTGCTTGTCA TGCTGCTCCT CCTGAGCCTG TGGGGGGCCC ACTACTACAG GACTCAGAAG 
CTGCTATCGA AAAACCCAAA GAATCCTTAT GAGGAGTCGA GCAGAGATGT GAGGAGTCGC 
AGGCCTGCTG ACACTGAGGA TGGGATGTCC TCTTGCCCTC AACCTTGGTT TGTGGTTATA 
AAAGAACACC AAGACCTCAA GAATGGGGGT CAACCAGTGG CTGGTGAGGA TGGCCAGGCA 
GCAGATGGGT CAATGCAACC AACTTCATGG AGGCAGGAGC CCCAGTTATG TGGAATGGGC 
ACAGAGCAAG GCTGCTGGAT TCCAGTATCC AGTGATAAGG GCTCCTGTCC CCAGGTAATG 
GAGCGAAGCT TTCATATGCC CTCCTATGGG ACACAGACCC TTGAAGGGGG TGTCGAGAAG 
CCCCATTCTC TCCTATCAGC TAACCCATTA TGGCAACAAA GGGCCCTGGA CCCACCACAC 
CAAATGGAGC TGACTCAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EGF ( XM_005262796.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_005262853.1 Homo sapiens epidermal growth factor (EGF), transcript variant X2, mRNA.

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CloneID	OHu38745
Clone ID Related Accession (Same CDS sequence)	XM_005262796.2
Accession Version	XM_005262796.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3648bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	pro-epidermal growth factor isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced XM_005262796.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCTCA CTCTTATCAT TCTGTTGCCA GTAGTTTCAA AATTTAGTTT TGTTAGTCTC 
TCAGCACCGC AGCACTGGAG CTGTCCTGAA GGTACTCTCG CAGGAAATGG GAATTCTACT 
TGTGTGGGTC CTGCACCCTT CTTAATTTTC TCCCATGGAA ATAGTATCTT TAGGATTGAC 
ACAGAAGGAA CCAATTATGA GCAATTGGTG GTGGATGCTG GTGTCTCAGT GATCATGGAT 
TTTCATTATA ATGAGAAAAG AATCTATTGG GTGGATTTAG AAAGACAACT TTTGCAAAGA 
GTTTTTCTGA ATGGGTCAAG GCAAGAGAGA GTATGTAATA TAGAGAAAAA TGTTTCTGGA 
ATGGCAATAA ATTGGATAAA TGAAGAAGTT ATTTGGTCAA ATCAACAGGA AGGAATCATT 
ACAGTAACAG ATATGAAAGG AAATAATTCC CACATTCTTT TAAGTGCTTT AAAATATCCT 
GCAAATGTAG CAGTTGATCC AGTAGAAAGG TTTATATTTT GGTCTTCAGA GGTGGCTGGA 
AGCCTTTATA GAGCAGATCT CGATGGTGTG GGAGTGAAGG CTCTGTTGGA GACATCAGAG 
AAAATAACAG CTGTGTCATT GGATGTGCTT GATAAGCGGC TGTTTTGGAT TCAGTACAAC 
AGAGAAGGAA GCAATTCTCT TATTTGCTCC TGTGATTATG ATGGAGGTTC TGTCCACATT 
AGTAAACATC CAACACAGCA TAATTTGTTT GCAATGTCCC TTTTTGGTGA CCGTATCTTC 
TATTCAACAT GGAAAATGAA GACAATTTGG ATAGCCAACA AACACACTGG AAAGGACATG 
GTTAGAATTA ACCTCCATTC ATCATTTGTA CCACTTGGTG AACTGAAAGT AGTGCATCCA 
CTTGCACAAC CCAAGGCAGA AGATGACACT TGGGAGCCTG AGCAGAAACT TTGCAAATTG 
AGGAAAGGAA ACTGCAGCAG CACTGTGTGT GGGCAAGACC TCCAGTCACA CTTGTGCATG 
TGTGCAGAGG GATACGCCCT AAGTCGAGAC CGGAAGTACT GTGAAGATGT TAATGAATGT 
GCTTTTTGGA ATCATGGCTG TACTCTTGGG TGTAAAAACA CCCCTGGATC CTATTACTGC 
ACGTGCCCTG TAGGATTTGT TCTGCTTCCT GATGGGAAAC GATGTCATCA ACTTGTTTCC 
TGTCCACGCA ATGTGTCTGA ATGCAGCCAT GACTGTGTTC TGACATCAGA AGGTCCCTTA 
TGTTTCTGTC CTGAAGGCTC AGTGCTTGAG AGAGATGGGA AAACATGTAG CGGTTGTTCC 
TCACCCGATA ATGGTGGATG TAGCCAGCTC TGCGTTCCTC TTAGCCCAGT ATCCTGGGAA 
TGTGATTGCT TTCCTGGGTA TGACCTACAA CTGGATGAAA AAAGCTGTGC AGCTTCAGGA 
CCACAACCAT TTTTGCTGTT TGCCAATTCT CAAGATATTC GACACATGCA TTTTGATGGA 
ACAGACTATG GAACTCTGCT CAGCCAGCAG ATGGGAATGG TTTATGCCCT AGATCATGAC 
CCTGTGGAAA ATAAGATATA CTTTGCCCAT ACAGCCCTGA AGTGGATAGA GAGAGCTAAT 
ATGGATGGTT CCCAGCGAGA AAGGCTTATT GAGGAAGGAG TAGATGTGCC AGAAGGTCTT 
GCTGTGGACT GGATTGGCCG TAGATTCTAT TGGACAGACA GAGGGAAATC TCTGATTGGA 
AGGAGTGATT TAAATGGGAA ACGTTCCAAA ATAATCACTA AGGAGAACAT CTCTCAACCA 
CGAGGAATTG CTGTTCATCC AATGGCCAAG AGATTATTCT GGACTGATAC AGGGATTAAT 
CCACGAATTG AAAGTTCTTC CCTCCAAGGC CTTGGCCGTC TGGTTATAGC CAGCTCTGAT 
CTAATCTGGC CCAGTGGAAT AACGATTGAC TTCTTAACTG ACAAGTTGTA CTGGTGCGAT 
GCCAAGCAGT CTGTGATTGA AATGGCCAAT CTGGATGGTT CAAAACGCCG AAGACTTACC 
CAGAATGATG TAGGTCACCC ATTTGCTGTA GCAGTGTTTG AGGATTATGT GTGGTTCTCA 
GATTGGGCTA TGCCATCAGT AATGAGAGTA AACAAGAGGA CTGGCAAAGA TAGAGTACGT 
CTCCAAGGCA GCATGCTGAA GCCCTCATCA CTGGTTGTGG TTCATCCATT GGCAAAACCA 
GGAGCAGATC CCTGCTTATA TCAAAACGGA GGCTGTGAAC ATATTTGCAA AAAGAGGCTT 
GGAACTGCTT GGTGTTCGTG TCGTGAAGGT TTTATGAAAG CCTCAGATGG GAAAACGTGT 
CTGGCTCTGG ATGGTCATCA GCTGTTGGCA GGTGGTGAAG TTGATCTAAA GAACCAAGTA 
ACACCATTGG ACATCTTGTC CAAGACTAGA GTGTCAGAAG ATAACATTAC AGAATCTCAA 
CACATGCTAG TGGCTGAAAT CATGGTGTCA GATCAAGATG ACTGTGCTCC TGTGGGATGC 
AGCATGTATG CTCGGTGTAT TTCAGAGGGA GAGGATGCCA CATGTCAGTG TTTGAAAGGA 
TTTGCTGGGG ATGGAAAACT ATGTTCTGAT ATAGATGAAT GTGAGATGGG TGTCCCAGTG 
TGCCCCCCTG CCTCCTCCAA GTGCATCAAC ACCGAAGGTG GTTATGTCTG CCGGTGCTCA 
GAAGGCTACC AAGGAGATGG GATTCACTGT CTTGATATTG ATGAGTGCCA ACTGGGGGAG 
CACAGCTGTG GAGAGAATGC CAGCTGCACA AATACAGAGG GAGGCTATAC CTGCATGTGT 
GCTGGACGCC TGTCTGAACC AGGACTGATT TGCCCTGACT CTACTCCACC CCCTCACCTC 
AGGGAAGATG ACCACCACTA TTCCGTAAGA AATAGTGACT CTGAATGTCC CCTGTCCCAC 
GATGGGTACT GCCTCCATGA TGGTGTGTGC ATGTATATTG AAGCATTGGA CAAGTATGCA 
TGCAACTGTG TTGTTGGCTA CATCGGGGAG CGATGTCAGT ACCGAGACCT GAAGTGGTGG 
GAACTGCGCC ACGCTGGCCA CGGGCAGCAG CAGAAGGTCA TCGTGGTGGC TGTCTGCGTG 
GTGGTGCTTG TCATGCTGCT CCTCCTGAGC CTGTGGGGGG CCCACTACTA CAGGACTCAG 
AAGCTGCTAT CGAAAAACCC AAAGAATCCT TATGAGGAGT CGAGCAGAGA TGTGAGGAGT 
CGCAGGCCTG CTGACACTGA GGATGGGATG TCCTCTTGCC CTCAACCTTG GTTTGTGGTT 
ATAAAAGAAC ACCAAGACCT CAAGAATGGG GGTCAACCAG TGGCTGGTGA GGATGGCCAG 
GCAGCAGATG GTCAGTTTTT ATCCCTGGCT CCTGGGTCAA TGCAACCAAC TTCATGGAGG 
CAGGAGCCCC AGTTATGTGG AATGGGCACA GAGCAAGGCT GCTGGATTCC AGTATCCAGT 
GATAAGGGCT CCTGTCCCCA GGTAATGGAG CGAAGCTTTC ATATGCCCTC CTATGGGACA 
CAGACCCTTG AAGGGGGTGT CGAGAAGCCC CATTCTCTCC TATCAGCTAA CCCATTATGG 
CAACAAAGGG CCCTGGACCC ACCACACCAA ATGGAGCTGA CTCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005262853.1
CDS	454..4101
Translation	MLLTLIILLPVVSKFSFVSLSAPQHWSCPEGTLAGNGNSTCVGPAPFLIFSHGNSIFRIDTEGTNYEQLVVDAGVSVIMDFHYNEKRIYWVDLERQLLQRVFLNGSRQERVCNIEKNVSGMAINWINEEVIWSNQQEGIITVTDMKGNNSHILLSALKYPANVAVDPVERFIFWSSEVAGSLYRADLDGVGVKALLETSEKITAVSLDVLDKRLFWIQYNREGSNSLICSCDYDGGSVHISKHPTQHNLFAMSLFGDRIFYSTWKMKTIWIANKHTGKDMVRINLHSSFVPLGELKVVHPLAQPKAEDDTWEPEQKLCKLRKGNCSSTVCGQDLQSHLCMCAEGYALSRDRKYCEDVNECAFWNHGCTLGCKNTPGSYYCTCPVGFVLLPDGKRCHQLVSCPRNVSECSHDCVLTSEGPLCFCPEGSVLERDGKTCSGCSSPDNGGCSQLCVPLSPVSWECDCFPGYDLQLDEKSCAASGPQPFLLFANSQDIRHMHFDGTDYGTLLSQQMGMVYALDHDPVENKIYFAHTALKWIERANMDGSQRERLIEEGVDVPEGLAVDWIGRRFYWTDRGKSLIGRSDLNGKRSKIITKENISQPRGIAVHPMAKRLFWTDTGINPRIESSSLQGLGRLVIASSDLIWPSGITIDFLTDKLYWCDAKQSVIEMANLDGSKRRRLTQNDVGHPFAVAVFEDYVWFSDWAMPSVMRVNKRTGKDRVRLQGSMLKPSSLVVVHPLAKPGADPCLYQNGGCEHICKKRLGTAWCSCREGFMKASDGKTCLALDGHQLLAGGEVDLKNQVTPLDILSKTRVSEDNITESQHMLVAEIMVSDQDDCAPVGCSMYARCISEGEDATCQCLKGFAGDGKLCSDIDECEMGVPVCPPASSKCINTEGGYVCRCSEGYQGDGIHCLDIDECQLGEHSCGENASCTNTEGGYTCMCAGRLSEPGLICPDSTPPPHLREDDHHYSVRNSDSECPLSHDGYCLHDGVCMYIEALDKYACNCVVGYIGERCQYRDLKWWELRHAGHGQQQKVIVVAVCVVVLVMLLLLSLWGAHYYRTQKLLSKNPKNPYEESSRDVRSRRPADTEDGMSSCPQPWFVVIKEHQDLKNGGQPVAGEDGQAADGQFLSLAPGSMQPTSWRQEPQLCGMGTEQGCWIPVSSDKGSCPQVMERSFHMPSYGTQTLEGGVEKPHSLLSANPLWQQRALDPPHQMELTQ

Target ORF information:

RefSeq Version	XM_005262796.2
Organism	Homo sapiens(human)
Definition	Homo sapiens epidermal growth factor (EGF), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005262796.2

ORF Insert Sequence:

ATGCTGCTCA CTCTTATCAT TCTGTTGCCA GTAGTTTCAA AATTTAGTTT TGTTAGTCTC 
TCAGCACCGC AGCACTGGAG CTGTCCTGAA GGTACTCTCG CAGGAAATGG GAATTCTACT 
TGTGTGGGTC CTGCACCCTT CTTAATTTTC TCCCATGGAA ATAGTATCTT TAGGATTGAC 
ACAGAAGGAA CCAATTATGA GCAATTGGTG GTGGATGCTG GTGTCTCAGT GATCATGGAT 
TTTCATTATA ATGAGAAAAG AATCTATTGG GTGGATTTAG AAAGACAACT TTTGCAAAGA 
GTTTTTCTGA ATGGGTCAAG GCAAGAGAGA GTATGTAATA TAGAGAAAAA TGTTTCTGGA 
ATGGCAATAA ATTGGATAAA TGAAGAAGTT ATTTGGTCAA ATCAACAGGA AGGAATCATT 
ACAGTAACAG ATATGAAAGG AAATAATTCC CACATTCTTT TAAGTGCTTT AAAATATCCT 
GCAAATGTAG CAGTTGATCC AGTAGAAAGG TTTATATTTT GGTCTTCAGA GGTGGCTGGA 
AGCCTTTATA GAGCAGATCT CGATGGTGTG GGAGTGAAGG CTCTGTTGGA GACATCAGAG 
AAAATAACAG CTGTGTCATT GGATGTGCTT GATAAGCGGC TGTTTTGGAT TCAGTACAAC 
AGAGAAGGAA GCAATTCTCT TATTTGCTCC TGTGATTATG ATGGAGGTTC TGTCCACATT 
AGTAAACATC CAACACAGCA TAATTTGTTT GCAATGTCCC TTTTTGGTGA CCGTATCTTC 
TATTCAACAT GGAAAATGAA GACAATTTGG ATAGCCAACA AACACACTGG AAAGGACATG 
GTTAGAATTA ACCTCCATTC ATCATTTGTA CCACTTGGTG AACTGAAAGT AGTGCATCCA 
CTTGCACAAC CCAAGGCAGA AGATGACACT TGGGAGCCTG AGCAGAAACT TTGCAAATTG 
AGGAAAGGAA ACTGCAGCAG CACTGTGTGT GGGCAAGACC TCCAGTCACA CTTGTGCATG 
TGTGCAGAGG GATACGCCCT AAGTCGAGAC CGGAAGTACT GTGAAGATGT TAATGAATGT 
GCTTTTTGGA ATCATGGCTG TACTCTTGGG TGTAAAAACA CCCCTGGATC CTATTACTGC 
ACGTGCCCTG TAGGATTTGT TCTGCTTCCT GATGGGAAAC GATGTCATCA ACTTGTTTCC 
TGTCCACGCA ATGTGTCTGA ATGCAGCCAT GACTGTGTTC TGACATCAGA AGGTCCCTTA 
TGTTTCTGTC CTGAAGGCTC AGTGCTTGAG AGAGATGGGA AAACATGTAG CGGTTGTTCC 
TCACCCGATA ATGGTGGATG TAGCCAGCTC TGCGTTCCTC TTAGCCCAGT ATCCTGGGAA 
TGTGATTGCT TTCCTGGGTA TGACCTACAA CTGGATGAAA AAAGCTGTGC AGCTTCAGGA 
CCACAACCAT TTTTGCTGTT TGCCAATTCT CAAGATATTC GACACATGCA TTTTGATGGA 
ACAGACTATG GAACTCTGCT CAGCCAGCAG ATGGGAATGG TTTATGCCCT AGATCATGAC 
CCTGTGGAAA ATAAGATATA CTTTGCCCAT ACAGCCCTGA AGTGGATAGA GAGAGCTAAT 
ATGGATGGTT CCCAGCGAGA AAGGCTTATT GAGGAAGGAG TAGATGTGCC AGAAGGTCTT 
GCTGTGGACT GGATTGGCCG TAGATTCTAT TGGACAGACA GAGGGAAATC TCTGATTGGA 
AGGAGTGATT TAAATGGGAA ACGTTCCAAA ATAATCACTA AGGAGAACAT CTCTCAACCA 
CGAGGAATTG CTGTTCATCC AATGGCCAAG AGATTATTCT GGACTGATAC AGGGATTAAT 
CCACGAATTG AAAGTTCTTC CCTCCAAGGC CTTGGCCGTC TGGTTATAGC CAGCTCTGAT 
CTAATCTGGC CCAGTGGAAT AACGATTGAC TTCTTAACTG ACAAGTTGTA CTGGTGCGAT 
GCCAAGCAGT CTGTGATTGA AATGGCCAAT CTGGATGGTT CAAAACGCCG AAGACTTACC 
CAGAATGATG TAGGTCACCC ATTTGCTGTA GCAGTGTTTG AGGATTATGT GTGGTTCTCA 
GATTGGGCTA TGCCATCAGT AATGAGAGTA AACAAGAGGA CTGGCAAAGA TAGAGTACGT 
CTCCAAGGCA GCATGCTGAA GCCCTCATCA CTGGTTGTGG TTCATCCATT GGCAAAACCA 
GGAGCAGATC CCTGCTTATA TCAAAACGGA GGCTGTGAAC ATATTTGCAA AAAGAGGCTT 
GGAACTGCTT GGTGTTCGTG TCGTGAAGGT TTTATGAAAG CCTCAGATGG GAAAACGTGT 
CTGGCTCTGG ATGGTCATCA GCTGTTGGCA GGTGGTGAAG TTGATCTAAA GAACCAAGTA 
ACACCATTGG ACATCTTGTC CAAGACTAGA GTGTCAGAAG ATAACATTAC AGAATCTCAA 
CACATGCTAG TGGCTGAAAT CATGGTGTCA GATCAAGATG ACTGTGCTCC TGTGGGATGC 
AGCATGTATG CTCGGTGTAT TTCAGAGGGA GAGGATGCCA CATGTCAGTG TTTGAAAGGA 
TTTGCTGGGG ATGGAAAACT ATGTTCTGAT ATAGATGAAT GTGAGATGGG TGTCCCAGTG 
TGCCCCCCTG CCTCCTCCAA GTGCATCAAC ACCGAAGGTG GTTATGTCTG CCGGTGCTCA 
GAAGGCTACC AAGGAGATGG GATTCACTGT CTTGATATTG ATGAGTGCCA ACTGGGGGAG 
CACAGCTGTG GAGAGAATGC CAGCTGCACA AATACAGAGG GAGGCTATAC CTGCATGTGT 
GCTGGACGCC TGTCTGAACC AGGACTGATT TGCCCTGACT CTACTCCACC CCCTCACCTC 
AGGGAAGATG ACCACCACTA TTCCGTAAGA AATAGTGACT CTGAATGTCC CCTGTCCCAC 
GATGGGTACT GCCTCCATGA TGGTGTGTGC ATGTATATTG AAGCATTGGA CAAGTATGCA 
TGCAACTGTG TTGTTGGCTA CATCGGGGAG CGATGTCAGT ACCGAGACCT GAAGTGGTGG 
GAACTGCGCC ACGCTGGCCA CGGGCAGCAG CAGAAGGTCA TCGTGGTGGC TGTCTGCGTG 
GTGGTGCTTG TCATGCTGCT CCTCCTGAGC CTGTGGGGGG CCCACTACTA CAGGACTCAG 
AAGCTGCTAT CGAAAAACCC AAAGAATCCT TATGAGGAGT CGAGCAGAGA TGTGAGGAGT 
CGCAGGCCTG CTGACACTGA GGATGGGATG TCCTCTTGCC CTCAACCTTG GTTTGTGGTT 
ATAAAAGAAC ACCAAGACCT CAAGAATGGG GGTCAACCAG TGGCTGGTGA GGATGGCCAG 
GCAGCAGATG GTCAGTTTTT ATCCCTGGCT CCTGGGTCAA TGCAACCAAC TTCATGGAGG 
CAGGAGCCCC AGTTATGTGG AATGGGCACA GAGCAAGGCT GCTGGATTCC AGTATCCAGT 
GATAAGGGCT CCTGTCCCCA GGTAATGGAG CGAAGCTTTC ATATGCCCTC CTATGGGACA 
CAGACCCTTG AAGGGGGTGT CGAGAAGCCC CATTCTCTCC TATCAGCTAA CCCATTATGG 
CAACAAAGGG CCCTGGACCC ACCACACCAA ATGGAGCTGA CTCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EGF ( XM_011531707.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011530009.1 Homo sapiens epidermal growth factor (EGF), transcript variant X6, mRNA.

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CloneID	OHu57444
Clone ID Related Accession (Same CDS sequence)	XM_011531707.1
Accession Version	XM_011531707.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3537bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	pro-epidermal growth factor isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAAAGA TTTCATGTCC TGCACCCTTC TTAATTTTCT CCCATGGAAA TAGTATCTTT 
AGGATTGACA CAGAAGGAAC CAATTATGAG CAATTGGTGG TGGATGCTGG TGTCTCAGTG 
ATCATGGATT TTCATTATAA TGAGAAAAGA ATCTATTGGG TGGATTTAGA AAGACAACTT 
TTGCAAAGAG TTTTTCTGAA TGGGTCAAGG CAAGAGAGAG TATGTAATAT AGAGAAAAAT 
GTTTCTGGAA TGGCAATAAA TTGGATAAAT GAAGAAGTTA TTTGGTCAAA TCAACAGGAA 
GGAATCATTA CAGTAACAGA TATGAAAGGA AATAATTCCC ACATTCTTTT AAGTGCTTTA 
AAATATCCTG CAAATGTAGC AGTTGATCCA GTAGAAAGGT TTATATTTTG GTCTTCAGAG 
GTGGCTGGAA GCCTTTATAG AGCAGATCTC GATGGTGTGG GAGTGAAGGC TCTGTTGGAG 
ACATCAGAGA AAATAACAGC TGTGTCATTG GATGTGCTTG ATAAGCGGCT GTTTTGGATT 
CAGTACAACA GAGAAGGAAG CAATTCTCTT ATTTGCTCCT GTGATTATGA TGGAGGTTCT 
GTCCACATTA GTAAACATCC AACACAGCAT AATTTGTTTG CAATGTCCCT TTTTGGTGAC 
CGTATCTTCT ATTCAACATG GAAAATGAAG ACAATTTGGA TAGCCAACAA ACACACTGGA 
AAGGACATGG TTAGAATTAA CCTCCATTCA TCATTTGTAC CACTTGGTGA ACTGAAAGTA 
GTGCATCCAC TTGCACAACC CAAGGCAGAA GATGACACTT GGGAGCCTGA GCAGAAACTT 
TGCAAATTGA GGAAAGGAAA CTGCAGCAGC ACTGTGTGTG GGCAAGACCT CCAGTCACAC 
TTGTGCATGT GTGCAGAGGG ATACGCCCTA AGTCGAGACC GGAAGTACTG TGAAGATGTT 
AATGAATGTG CTTTTTGGAA TCATGGCTGT ACTCTTGGGT GTAAAAACAC CCCTGGATCC 
TATTACTGCA CGTGCCCTGT AGGATTTGTT CTGCTTCCTG ATGGGAAACG ATGTCATCAA 
CTTGTTTCCT GTCCACGCAA TGTGTCTGAA TGCAGCCATG ACTGTGTTCT GACATCAGAA 
GGTCCCTTAT GTTTCTGTCC TGAAGGCTCA GTGCTTGAGA GAGATGGGAA AACATGTAGC 
GGTTGTTCCT CACCCGATAA TGGTGGATGT AGCCAGCTCT GCGTTCCTCT TAGCCCAGTA 
TCCTGGGAAT GTGATTGCTT TCCTGGGTAT GACCTACAAC TGGATGAAAA AAGCTGTGCA 
GCTTCAGGAC CACAACCATT TTTGCTGTTT GCCAATTCTC AAGATATTCG ACACATGCAT 
TTTGATGGAA CAGACTATGG AACTCTGCTC AGCCAGCAGA TGGGAATGGT TTATGCCCTA 
GATCATGACC CTGTGGAAAA TAAGATATAC TTTGCCCATA CAGCCCTGAA GTGGATAGAG 
AGAGCTAATA TGGATGGTTC CCAGCGAGAA AGGCTTATTG AGGAAGGAGT AGATGTGCCA 
GAAGGTCTTG CTGTGGACTG GATTGGCCGT AGATTCTATT GGACAGACAG AGGGAAATCT 
CTGATTGGAA GGAGTGATTT AAATGGGAAA CGTTCCAAAA TAATCACTAA GGAGAACATC 
TCTCAACCAC GAGGAATTGC TGTTCATCCA ATGGCCAAGA GATTATTCTG GACTGATACA 
GGGATTAATC CACGAATTGA AAGTTCTTCC CTCCAAGGCC TTGGCCGTCT GGTTATAGCC 
AGCTCTGATC TAATCTGGCC CAGTGGAATA ACGATTGACT TCTTAACTGA CAAGTTGTAC 
TGGTGCGATG CCAAGCAGTC TGTGATTGAA ATGGCCAATC TGGATGGTTC AAAACGCCGA 
AGACTTACCC AGAATGATGT AGGTCACCCA TTTGCTGTAG CAGTGTTTGA GGATTATGTG 
TGGTTCTCAG ATTGGGCTAT GCCATCAGTA ATGAGAGTAA ACAAGAGGAC TGGCAAAGAT 
AGAGTACGTC TCCAAGGCAG CATGCTGAAG CCCTCATCAC TGGTTGTGGT TCATCCATTG 
GCAAAACCAG GAGCAGATCC CTGCTTATAT CAAAACGGAG GCTGTGAACA TATTTGCAAA 
AAGAGGCTTG GAACTGCTTG GTGTTCGTGT CGTGAAGGTT TTATGAAAGC CTCAGATGGG 
AAAACGTGTC TGGCTCTGGA TGGTCATCAG CTGTTGGCAG GTGGTGAAGT TGATCTAAAG 
AACCAAGTAA CACCATTGGA CATCTTGTCC AAGACTAGAG TGTCAGAAGA TAACATTACA 
GAATCTCAAC ACATGCTAGT GGCTGAAATC ATGGTGTCAG ATCAAGATGA CTGTGCTCCT 
GTGGGATGCA GCATGTATGC TCGGTGTATT TCAGAGGGAG AGGATGCCAC ATGTCAGTGT 
TTGAAAGGAT TTGCTGGGGA TGGAAAACTA TGTTCTGATA TAGATGAATG TGAGATGGGT 
GTCCCAGTGT GCCCCCCTGC CTCCTCCAAG TGCATCAACA CCGAAGGTGG TTATGTCTGC 
CGGTGCTCAG AAGGCTACCA AGGAGATGGG ATTCACTGTC TTGATATTGA TGAGTGCCAA 
CTGGGGGAGC ACAGCTGTGG AGAGAATGCC AGCTGCACAA ATACAGAGGG AGGCTATACC 
TGCATGTGTG CTGGACGCCT GTCTGAACCA GGACTGATTT GCCCTGACTC TACTCCACCC 
CCTCACCTCA GGGAAGATGA CCACCACTAT TCCGTAAGAA ATAGTGACTC TGAATGTCCC 
CTGTCCCACG ATGGGTACTG CCTCCATGAT GGTGTGTGCA TGTATATTGA AGCATTGGAC 
AAGTATGCAT GCAACTGTGT TGTTGGCTAC ATCGGGGAGC GATGTCAGTA CCGAGACCTG 
AAGTGGTGGG AACTGCGCCA CGCTGGCCAC GGGCAGCAGC AGAAGGTCAT CGTGGTGGCT 
GTCTGCGTGG TGGTGCTTGT CATGCTGCTC CTCCTGAGCC TGTGGGGGGC CCACTACTAC 
AGGACTCAGA AGCTGCTATC GAAAAACCCA AAGAATCCTT ATGAGGAGTC GAGCAGAGAT 
GTGAGGAGTC GCAGGCCTGC TGACACTGAG GATGGGATGT CCTCTTGCCC TCAACCTTGG 
TTTGTGGTTA TAAAAGAACA CCAAGACCTC AAGAATGGGG GTCAACCAGT GGCTGGTGAG 
GATGGCCAGG CAGCAGATGG TCAGTTTTTA TCCCTGGCTC CTGGGTCAAT GCAACCAACT 
TCATGGAGGC AGGAGCCCCA GTTATGTGGA ATGGGCACAG AGCAAGGCTG CTGGATTCCA 
GTATCCAGTG ATAAGGGCTC CTGTCCCCAG GTAATGGAGC GAAGCTTTCA TATGCCCTCC 
TATGGGACAC AGACCCTTGA AGGGGGTGTC GAGAAGCCCC ATTCTCTCCT ATCAGCTAAC 
CCATTATGGC AACAAAGGGC CCTGGACCCA CCACACCAAA TGGAGCTGAC TCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011530009.1
CDS	214..3750
Translation	MAKISCPAPFLIFSHGNSIFRIDTEGTNYEQLVVDAGVSVIMDFHYNEKRIYWVDLERQLLQRVFLNGSRQERVCNIEKNVSGMAINWINEEVIWSNQQEGIITVTDMKGNNSHILLSALKYPANVAVDPVERFIFWSSEVAGSLYRADLDGVGVKALLETSEKITAVSLDVLDKRLFWIQYNREGSNSLICSCDYDGGSVHISKHPTQHNLFAMSLFGDRIFYSTWKMKTIWIANKHTGKDMVRINLHSSFVPLGELKVVHPLAQPKAEDDTWEPEQKLCKLRKGNCSSTVCGQDLQSHLCMCAEGYALSRDRKYCEDVNECAFWNHGCTLGCKNTPGSYYCTCPVGFVLLPDGKRCHQLVSCPRNVSECSHDCVLTSEGPLCFCPEGSVLERDGKTCSGCSSPDNGGCSQLCVPLSPVSWECDCFPGYDLQLDEKSCAASGPQPFLLFANSQDIRHMHFDGTDYGTLLSQQMGMVYALDHDPVENKIYFAHTALKWIERANMDGSQRERLIEEGVDVPEGLAVDWIGRRFYWTDRGKSLIGRSDLNGKRSKIITKENISQPRGIAVHPMAKRLFWTDTGINPRIESSSLQGLGRLVIASSDLIWPSGITIDFLTDKLYWCDAKQSVIEMANLDGSKRRRLTQNDVGHPFAVAVFEDYVWFSDWAMPSVMRVNKRTGKDRVRLQGSMLKPSSLVVVHPLAKPGADPCLYQNGGCEHICKKRLGTAWCSCREGFMKASDGKTCLALDGHQLLAGGEVDLKNQVTPLDILSKTRVSEDNITESQHMLVAEIMVSDQDDCAPVGCSMYARCISEGEDATCQCLKGFAGDGKLCSDIDECEMGVPVCPPASSKCINTEGGYVCRCSEGYQGDGIHCLDIDECQLGEHSCGENASCTNTEGGYTCMCAGRLSEPGLICPDSTPPPHLREDDHHYSVRNSDSECPLSHDGYCLHDGVCMYIEALDKYACNCVVGYIGERCQYRDLKWWELRHAGHGQQQKVIVVAVCVVVLVMLLLLSLWGAHYYRTQKLLSKNPKNPYEESSRDVRSRRPADTEDGMSSCPQPWFVVIKEHQDLKNGGQPVAGEDGQAADGQFLSLAPGSMQPTSWRQEPQLCGMGTEQGCWIPVSSDKGSCPQVMERSFHMPSYGTQTLEGGVEKPHSLLSANPLWQQRALDPPHQMELTQ

Target ORF information:

RefSeq Version	XM_011531707.1
Organism	Homo sapiens(human)
Definition	Homo sapiens epidermal growth factor (EGF), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011531707.1

ORF Insert Sequence:

ATGGCAAAGA TTTCATGTCC TGCACCCTTC TTAATTTTCT CCCATGGAAA TAGTATCTTT 
AGGATTGACA CAGAAGGAAC CAATTATGAG CAATTGGTGG TGGATGCTGG TGTCTCAGTG 
ATCATGGATT TTCATTATAA TGAGAAAAGA ATCTATTGGG TGGATTTAGA AAGACAACTT 
TTGCAAAGAG TTTTTCTGAA TGGGTCAAGG CAAGAGAGAG TATGTAATAT AGAGAAAAAT 
GTTTCTGGAA TGGCAATAAA TTGGATAAAT GAAGAAGTTA TTTGGTCAAA TCAACAGGAA 
GGAATCATTA CAGTAACAGA TATGAAAGGA AATAATTCCC ACATTCTTTT AAGTGCTTTA 
AAATATCCTG CAAATGTAGC AGTTGATCCA GTAGAAAGGT TTATATTTTG GTCTTCAGAG 
GTGGCTGGAA GCCTTTATAG AGCAGATCTC GATGGTGTGG GAGTGAAGGC TCTGTTGGAG 
ACATCAGAGA AAATAACAGC TGTGTCATTG GATGTGCTTG ATAAGCGGCT GTTTTGGATT 
CAGTACAACA GAGAAGGAAG CAATTCTCTT ATTTGCTCCT GTGATTATGA TGGAGGTTCT 
GTCCACATTA GTAAACATCC AACACAGCAT AATTTGTTTG CAATGTCCCT TTTTGGTGAC 
CGTATCTTCT ATTCAACATG GAAAATGAAG ACAATTTGGA TAGCCAACAA ACACACTGGA 
AAGGACATGG TTAGAATTAA CCTCCATTCA TCATTTGTAC CACTTGGTGA ACTGAAAGTA 
GTGCATCCAC TTGCACAACC CAAGGCAGAA GATGACACTT GGGAGCCTGA GCAGAAACTT 
TGCAAATTGA GGAAAGGAAA CTGCAGCAGC ACTGTGTGTG GGCAAGACCT CCAGTCACAC 
TTGTGCATGT GTGCAGAGGG ATACGCCCTA AGTCGAGACC GGAAGTACTG TGAAGATGTT 
AATGAATGTG CTTTTTGGAA TCATGGCTGT ACTCTTGGGT GTAAAAACAC CCCTGGATCC 
TATTACTGCA CGTGCCCTGT AGGATTTGTT CTGCTTCCTG ATGGGAAACG ATGTCATCAA 
CTTGTTTCCT GTCCACGCAA TGTGTCTGAA TGCAGCCATG ACTGTGTTCT GACATCAGAA 
GGTCCCTTAT GTTTCTGTCC TGAAGGCTCA GTGCTTGAGA GAGATGGGAA AACATGTAGC 
GGTTGTTCCT CACCCGATAA TGGTGGATGT AGCCAGCTCT GCGTTCCTCT TAGCCCAGTA 
TCCTGGGAAT GTGATTGCTT TCCTGGGTAT GACCTACAAC TGGATGAAAA AAGCTGTGCA 
GCTTCAGGAC CACAACCATT TTTGCTGTTT GCCAATTCTC AAGATATTCG ACACATGCAT 
TTTGATGGAA CAGACTATGG AACTCTGCTC AGCCAGCAGA TGGGAATGGT TTATGCCCTA 
GATCATGACC CTGTGGAAAA TAAGATATAC TTTGCCCATA CAGCCCTGAA GTGGATAGAG 
AGAGCTAATA TGGATGGTTC CCAGCGAGAA AGGCTTATTG AGGAAGGAGT AGATGTGCCA 
GAAGGTCTTG CTGTGGACTG GATTGGCCGT AGATTCTATT GGACAGACAG AGGGAAATCT 
CTGATTGGAA GGAGTGATTT AAATGGGAAA CGTTCCAAAA TAATCACTAA GGAGAACATC 
TCTCAACCAC GAGGAATTGC TGTTCATCCA ATGGCCAAGA GATTATTCTG GACTGATACA 
GGGATTAATC CACGAATTGA AAGTTCTTCC CTCCAAGGCC TTGGCCGTCT GGTTATAGCC 
AGCTCTGATC TAATCTGGCC CAGTGGAATA ACGATTGACT TCTTAACTGA CAAGTTGTAC 
TGGTGCGATG CCAAGCAGTC TGTGATTGAA ATGGCCAATC TGGATGGTTC AAAACGCCGA 
AGACTTACCC AGAATGATGT AGGTCACCCA TTTGCTGTAG CAGTGTTTGA GGATTATGTG 
TGGTTCTCAG ATTGGGCTAT GCCATCAGTA ATGAGAGTAA ACAAGAGGAC TGGCAAAGAT 
AGAGTACGTC TCCAAGGCAG CATGCTGAAG CCCTCATCAC TGGTTGTGGT TCATCCATTG 
GCAAAACCAG GAGCAGATCC CTGCTTATAT CAAAACGGAG GCTGTGAACA TATTTGCAAA 
AAGAGGCTTG GAACTGCTTG GTGTTCGTGT CGTGAAGGTT TTATGAAAGC CTCAGATGGG 
AAAACGTGTC TGGCTCTGGA TGGTCATCAG CTGTTGGCAG GTGGTGAAGT TGATCTAAAG 
AACCAAGTAA CACCATTGGA CATCTTGTCC AAGACTAGAG TGTCAGAAGA TAACATTACA 
GAATCTCAAC ACATGCTAGT GGCTGAAATC ATGGTGTCAG ATCAAGATGA CTGTGCTCCT 
GTGGGATGCA GCATGTATGC TCGGTGTATT TCAGAGGGAG AGGATGCCAC ATGTCAGTGT 
TTGAAAGGAT TTGCTGGGGA TGGAAAACTA TGTTCTGATA TAGATGAATG TGAGATGGGT 
GTCCCAGTGT GCCCCCCTGC CTCCTCCAAG TGCATCAACA CCGAAGGTGG TTATGTCTGC 
CGGTGCTCAG AAGGCTACCA AGGAGATGGG ATTCACTGTC TTGATATTGA TGAGTGCCAA 
CTGGGGGAGC ACAGCTGTGG AGAGAATGCC AGCTGCACAA ATACAGAGGG AGGCTATACC 
TGCATGTGTG CTGGACGCCT GTCTGAACCA GGACTGATTT GCCCTGACTC TACTCCACCC 
CCTCACCTCA GGGAAGATGA CCACCACTAT TCCGTAAGAA ATAGTGACTC TGAATGTCCC 
CTGTCCCACG ATGGGTACTG CCTCCATGAT GGTGTGTGCA TGTATATTGA AGCATTGGAC 
AAGTATGCAT GCAACTGTGT TGTTGGCTAC ATCGGGGAGC GATGTCAGTA CCGAGACCTG 
AAGTGGTGGG AACTGCGCCA CGCTGGCCAC GGGCAGCAGC AGAAGGTCAT CGTGGTGGCT 
GTCTGCGTGG TGGTGCTTGT CATGCTGCTC CTCCTGAGCC TGTGGGGGGC CCACTACTAC 
AGGACTCAGA AGCTGCTATC GAAAAACCCA AAGAATCCTT ATGAGGAGTC GAGCAGAGAT 
GTGAGGAGTC GCAGGCCTGC TGACACTGAG GATGGGATGT CCTCTTGCCC TCAACCTTGG 
TTTGTGGTTA TAAAAGAACA CCAAGACCTC AAGAATGGGG GTCAACCAGT GGCTGGTGAG 
GATGGCCAGG CAGCAGATGG TCAGTTTTTA TCCCTGGCTC CTGGGTCAAT GCAACCAACT 
TCATGGAGGC AGGAGCCCCA GTTATGTGGA ATGGGCACAG AGCAAGGCTG CTGGATTCCA 
GTATCCAGTG ATAAGGGCTC CTGTCCCCAG GTAATGGAGC GAAGCTTTCA TATGCCCTCC 
TATGGGACAC AGACCCTTGA AGGGGGTGTC GAGAAGCCCC ATTCTCTCCT ATCAGCTAAC 
CCATTATGGC AACAAAGGGC CCTGGACCCA CCACACCAAA TGGAGCTGAC TCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	EGF
Entrez Gene ID	1950
Full Name	epidermal growth factor
Synonyms	HOMG4,URG
General protein information	Preferred Names epidermal growth factor Names pro-epidermal growth factor beta-urogastrone
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 4 Map Location: 4q25
Summary	This gene encodes a member of the epidermal growth factor superfamily. The encoded preproprotein is proteolytically processed to generate the 53-amino acid epidermal growth factor peptide. This protein acts a potent mitogenic factor that plays an important role in the growth, proliferation and differentiation of numerous cell types. This protein acts by binding with high affinity to the cell surface receptor, epidermal growth factor receptor. Defects in this gene are the cause of hypomagnesemia type 4. Dysregulation of this gene has been associated with the growth and progression of certain cancers. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed. [provided by RefSeq, Jan 2016].
Disorder MIM:	131530
Disorder Html:	Hypomagnesemia 4, renal, 611718 (3)

Homo sapiens (human)	EGF	NP_001954.2
Bos taurus (cattle)	EGF	XP_002688149.2
Mus musculus (house mouse)	Egf	NP_034243.2
	EGF	XP_001088957.1
Rattus norvegicus (Norway rat)	Egf	NP_036974.1
Canis lupus familiaris (dog)	EGF	NP_001003094.1
Danio rerio (zebrafish)	egf	NP_991294.1
Pan troglodytes (chimpanzee)	EGF	XP_517395.2
Gallus gallus (chicken)	EGF	NP_001001292.1
Xenopus tropicalis (tropical clawed frog)	egf	NP_001106478.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

EGF cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

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