EFNA4 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	EFNA4
Entrez Gene ID	1945
Full Name	ephrin A4
Synonyms	EFL4,EPLG4,LERK4
General protein information	Preferred Names ephrin A4 Names ephrin-A4 LERK-4 eph-related receptor tyrosine kinase ligand 4 ligand of eph-related kinase 4
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 1 Map Location: 1q21.3
Summary	This gene encodes a member of the ephrin (EPH) family. The ephrins and EPH-related receptors comprise the largest subfamily of receptor protein-tyrosine kinases and have been implicated in mediating developmental events, especially in the nervous system and in erythropoiesis. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. This gene encodes an EFNA class ephrin. Three transcript variants that encode distinct proteins have been identified. [provided by RefSeq, Jul 2008].
Disorder MIM:	601380

mRNA and Protein(s)

mRNA	Protein	Name
NM_005227.2	NP_005218.1	ephrin-A4 isoform a precursor
NM_005227.3	NP_005218.1	ephrin-A4 isoform a precursor
NM_182689.2	NP_872631.1	ephrin-A4 isoform b precursor
NM_182690.3	NP_872632.2	ephrin-A4 isoform c precursor
NM_182690.2	NP_872632.2	ephrin-A4 isoform c precursor
NM_182689.1	NP_872631.1	ephrin-A4 isoform b precursor

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	LOC100003450	XP_001343018.4
Pan troglodytes (chimpanzee)	EFNA4	XP_003308471.1
Homo sapiens (human)	EFNA4	NP_005218.1
Canis lupus familiaris (dog)	EFNA4	XP_547555.2
Bos taurus (cattle)	EFNA4	NP_001094719.1
Mus musculus (house mouse)	Efna4	NP_031936.2
Rattus norvegicus (Norway rat)	Efna4	NP_001101162.1

Gene Ontology
Bibliography

Related articles in PubMed

EphrinA4 plays a critical role in ?4 and ?L mediated survival of human CLL cells during extravasation.
Flores MA, Fortea P, Trinidad EM, Garc?a D, Soler G, Ortu?o FJ, Zapata AG, Alonso-Colmenar LM
Oncotarget7(30)48481-48500(2016 Jul)

Ephrin-A3 and ephrin-A4 contribute to microglia-induced angiogenesis in brain endothelial cells.
Li Y, Liu DX, Li MY, Qin XX, Fang WG, Zhao WD, Chen YH
Anatomical record (Hoboken, N.J. : 2007)297(10)1908-18(2014 Oct)

Ephrin-As, Eph receptors and integrin ?3 interact and colocalise at membrane protrusions of U251MG glioblastoma cells.
Makarov A, Ylivinkka I, Nyman TA, Hyyti?inen M, Keski-Oja J
Cell biology international37(10)1080-8(2013 Oct)

Identification of 23 new prostate cancer susceptibility loci using the iCOGS custom genotyping array.
Eeles RA, Olama AA, Benlloch S, Saunders EJ, Leongamornlert DA, Tymrakiewicz M, Ghoussaini M, Luccarini C, Dennis J, Jugurnauth-Little S, Dadaev T, Neal DE, Hamdy FC, Donovan JL, Muir K, Giles GG, Severi G, Wiklund F, Gronberg H, Haiman CA, Schumacher F, Henderson BE, Le Marchand L, Lindstrom S, Kraft P, Hunter DJ, Gapstur S, Chanock SJ, Berndt SI, Albanes D, Andriole G, Schleutker J, Weischer M, Canzian F, Riboli E, Key TJ, Travis RC, Campa D, Ingles SA, John EM, Hayes RB, Pharoah PD, Pashayan N, Khaw KT, Stanford JL, Ostrander EA, Signorello LB, Thibodeau SN, Schaid D, Maier C, Vogel W, Kibel AS, Cybulski C, Lubinski J, Cannon-Albright L, Brenner H, Park JY, Kaneva R, Batra J, Spurdle AB, Clements JA, Teixeira MR, Dicks E, Lee A, Dunning AM, Baynes C, Conroy D, Maranian MJ, Ahmed S, Govindasami K, Guy M, Wilkinson RA, Sawyer EJ, Morgan A, Dearnaley DP, Horwich A, Huddart RA, Khoo VS, Parker CC, Van As NJ, Woodhouse CJ, Thompson A, Dudderidge T, Ogden C, Cooper CS, Lophatananon A, Cox A, Southey MC, Hopper JL, English DR, Aly M, Adolfsson J, Xu J, Zheng SL, Yeager M, Kaaks R, Diver WR, Gaudet MM, Stern MC, Corral R, Joshi AD, Shahabi A, Wahlfors T, Tammela TL, Auvinen A, Virtamo J, Klarskov P, Nordestgaard BG, R?der MA, Nielsen SF, Bojesen SE, Siddiq A, Fitzgerald LM, Kolb S, Kwon EM, Karyadi DM, Blot WJ, Zheng W, Cai Q, McDonnell SK, Rinckleb AE, Drake B, Colditz G, Wokolorczyk D, Stephenson RA, Teerlink C, Muller H, Rothenbacher D, Sellers TA, Lin HY, Slavov C, Mitev V, Lose F, Srinivasan S, Maia S, Paulo P, Lange E, Cooney KA, Antoniou AC, Vincent D, Bacot F, Tessier DC, , , , , , Kote-Jarai Z, Easton DF
Nature genetics45(4)385-91, 391e1-2(2013 Apr)

Primer-BLAST: a tool to design target-specific primers for polymerase chain reaction.
Ye J, Coulouris G, Zaretskaya I, Cutcutache I, Rozen S, Madden TL
BMC bioinformatics13134(2012 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Study present for the first time in vitro and in vivo evidence suggesting that the major role of two ephrin A4 isoforms in chronic lymphocytic leukemia could be related with a non-previously described mechanism of survival linked to extravasation strongly dependent on integrin signaling.
Title: EphrinA4 plays a critical role in ?4 and ?L mediated survival of human CLL cells during extravasation.

The present study provides evidence that microglia upregulates endothelial ephrin-A3 and ephrin-A4 to facilitate in vitro angiogenesis of brain endothelial cells, which is mediated by microglia-released TNF-alpha.
Title: Ephrin-A3 and ephrin-A4 contribute to microglia-induced angiogenesis in brain endothelial cells.

The interaction between ephrin-As, Eph receptors and integrin alpha3 is plausibly important for the crosstalk between Eph and integrin signalling pathways at the membrane protrusions and in the migration of brain cancer cells.
Title: Ephrin-As, Eph receptors and integrin ?3 interact and colocalise at membrane protrusions of U251MG glioblastoma cells.

Observational study of gene-disease association. (HuGE Navigator)
Title: Prevalence and complications of single-gene and chromosomal disorders in craniosynostosis.

The cytoplasmic pattern of ephrin A4 could identify a subgroup of primary osteosarcoma patients with a high liability for progression, poor prognosis, and inferior response to chemotherapy.
Title: Ephrin A4 expression in osteosarcoma, impact on prognosis, and patient outcome.

The following EFNA4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EFNA4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu18034	NM_005227.3 Latest version!	Homo sapiens ephrin A4 (EFNA4), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$188.30 $269.00
OHu02288	NM_182689.2 Latest version!	Homo sapiens ephrin A4 (EFNA4), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00
OHu30425	NM_182690.3 Latest version!	Homo sapiens ephrin A4 (EFNA4), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
View Old Accession Versions >>
OHu18034	NM_005227.2	Homo sapiens ephrin A4 (EFNA4), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$188.30 $269.00
OHu30425	NM_182690.2	Homo sapiens ephrin A4 (EFNA4), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OHu02288	NM_182689.1	Homo sapiens ephrin A4 (EFNA4), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

EFNA4 ( NM_005227.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_005218.1 Homo sapiens ephrin A4 (EFNA4), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu18034
Clone ID Related Accession (Same CDS sequence)	NM_005227.2 , NM_005227.3
Accession Version	NM_005227.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 606bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-30
Organism	Homo sapiens(human)
Product	ephrin-A4 isoform a precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CD671399.1, BE780161.1, AI459151.1 and BQ940212.1. This sequence is a reference standard in the RefSeqGene project. On Jul 31, 2003 this sequence version replaced NM_005227.1. Transcript Variant: This variant (1), also known as ephrin-A4 (m), is the longest transcript and it encodes isoform a. Isoform a is a membrane-bound protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: ERR279825.9236.1, SRR1163658.260846.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCGGCTGC TGCCCCTGCT GCGGACTGTC CTCTGGGCCG CGTTCCTCGG CTCCCCTCTG 
CGCGGGGGCT CCAGCCTCCG CCACGTAGTC TACTGGAACT CCAGTAACCC CAGGTTGCTT 
CGAGGAGACG CCGTGGTGGA GCTGGGCCTC AACGATTACC TAGACATTGT CTGCCCCCAC 
TACGAAGGCC CAGGGCCCCC TGAGGGCCCC GAGACGTTTG CTTTGTACAT GGTGGACTGG 
CCAGGCTATG AGTCCTGCCA GGCAGAGGGC CCCCGGGCCT ACAAGCGCTG GGTGTGCTCC 
CTGCCCTTTG GCCATGTTCA ATTCTCAGAG AAGATTCAGC GCTTCACACC CTTCTCCCTC 
GGCTTTGAGT TCTTACCTGG AGAGACTTAC TACTACATCT CGGTGCCCAC TCCAGAGAGT 
TCTGGCCAGT GCTTGAGGCT CCAGGTGTCT GTCTGCTGCA AGGAGAGGAA GTCTGAGTCA 
GCCCATCCTG TTGGGAGCCC TGGAGAGAGT GGCACATCAG GGTGGCGAGG GGGGGACACT 
CCCAGCCCCC TCTGTCTCTT GCTATTACTG CTGCTTCTGA TTCTTCGTCT TCTGCGAATT 
CTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005218.1
CDS	88..693
Translation	MRLLPLLRTVLWAAFLGSPLRGGSSLRHVVYWNSSNPRLLRGDAVVELGLNDYLDIVCPHYEGPGPPEGPETFALYMVDWPGYESCQAEGPRAYKRWVCSLPFGHVQFSEKIQRFTPFSLGFEFLPGETYYYISVPTPESSGQCLRLQVSVCCKERKSESAHPVGSPGESGTSGWRGGDTPSPLCLLLLLLLLILRLLRIL

Target ORF information:

RefSeq Version	NM_005227.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ephrin A4 (EFNA4), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005227.2

ORF Insert Sequence:

ATGCGGCTGC TGCCCCTGCT GCGGACTGTC CTCTGGGCCG CGTTCCTCGG CTCCCCTCTG 
CGCGGGGGCT CCAGCCTCCG CCACGTAGTC TACTGGAACT CCAGTAACCC CAGGTTGCTT 
CGAGGAGACG CCGTGGTGGA GCTGGGCCTC AACGATTACC TAGACATTGT CTGCCCCCAC 
TACGAAGGCC CAGGGCCCCC TGAGGGCCCC GAGACGTTTG CTTTGTACAT GGTGGACTGG 
CCAGGCTATG AGTCCTGCCA GGCAGAGGGC CCCCGGGCCT ACAAGCGCTG GGTGTGCTCC 
CTGCCCTTTG GCCATGTTCA ATTCTCAGAG AAGATTCAGC GCTTCACACC CTTCTCCCTC 
GGCTTTGAGT TCTTACCTGG AGAGACTTAC TACTACATCT CGGTGCCCAC TCCAGAGAGT 
TCTGGCCAGT GCTTGAGGCT CCAGGTGTCT GTCTGCTGCA AGGAGAGGAA GTCTGAGTCA 
GCCCATCCTG TTGGGAGCCC TGGAGAGAGT GGCACATCAG GGTGGCGAGG GGGGGACACT 
CCCAGCCCCC TCTGTCTCTT GCTATTACTG CTGCTTCTGA TTCTTCGTCT TCTGCGAATT 
CTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EFNA4 ( NM_005227.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_005218.1 Homo sapiens ephrin A4 (EFNA4), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu18034
Clone ID Related Accession (Same CDS sequence)	NM_005227.2 , NM_005227.3
Accession Version	NM_005227.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 606bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-25
Organism	Homo sapiens(human)
Product	ephrin-A4 isoform a precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CD671399.1, BE780161.1, AI459151.1 and BQ940212.1. This sequence is a reference standard in the RefSeqGene project. On Nov 22, 2018 this sequence version replaced NM_005227.2. Transcript Variant: This variant (1), also known as ephrin-A4 (m), is the longest transcript and it encodes isoform a. Isoform a is a membrane-bound protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: ERR279825.9236.1, SRR1163658.260846.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000368409.8/ ENSP00000357394.3 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGCGGCTGC TGCCCCTGCT GCGGACTGTC CTCTGGGCCG CGTTCCTCGG CTCCCCTCTG 
CGCGGGGGCT CCAGCCTCCG CCACGTAGTC TACTGGAACT CCAGTAACCC CAGGTTGCTT 
CGAGGAGACG CCGTGGTGGA GCTGGGCCTC AACGATTACC TAGACATTGT CTGCCCCCAC 
TACGAAGGCC CAGGGCCCCC TGAGGGCCCC GAGACGTTTG CTTTGTACAT GGTGGACTGG 
CCAGGCTATG AGTCCTGCCA GGCAGAGGGC CCCCGGGCCT ACAAGCGCTG GGTGTGCTCC 
CTGCCCTTTG GCCATGTTCA ATTCTCAGAG AAGATTCAGC GCTTCACACC CTTCTCCCTC 
GGCTTTGAGT TCTTACCTGG AGAGACTTAC TACTACATCT CGGTGCCCAC TCCAGAGAGT 
TCTGGCCAGT GCTTGAGGCT CCAGGTGTCT GTCTGCTGCA AGGAGAGGAA GTCTGAGTCA 
GCCCATCCTG TTGGGAGCCC TGGAGAGAGT GGCACATCAG GGTGGCGAGG GGGGGACACT 
CCCAGCCCCC TCTGTCTCTT GCTATTACTG CTGCTTCTGA TTCTTCGTCT TCTGCGAATT 
CTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005218.1
CDS	85..690
Translation	MRLLPLLRTVLWAAFLGSPLRGGSSLRHVVYWNSSNPRLLRGDAVVELGLNDYLDIVCPHYEGPGPPEGPETFALYMVDWPGYESCQAEGPRAYKRWVCSLPFGHVQFSEKIQRFTPFSLGFEFLPGETYYYISVPTPESSGQCLRLQVSVCCKERKSESAHPVGSPGESGTSGWRGGDTPSPLCLLLLLLLLILRLLRIL

Target ORF information:

RefSeq Version	NM_005227.3
Organism	Homo sapiens(human)
Definition	Homo sapiens ephrin A4 (EFNA4), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005227.3

ORF Insert Sequence:

ATGCGGCTGC TGCCCCTGCT GCGGACTGTC CTCTGGGCCG CGTTCCTCGG CTCCCCTCTG 
CGCGGGGGCT CCAGCCTCCG CCACGTAGTC TACTGGAACT CCAGTAACCC CAGGTTGCTT 
CGAGGAGACG CCGTGGTGGA GCTGGGCCTC AACGATTACC TAGACATTGT CTGCCCCCAC 
TACGAAGGCC CAGGGCCCCC TGAGGGCCCC GAGACGTTTG CTTTGTACAT GGTGGACTGG 
CCAGGCTATG AGTCCTGCCA GGCAGAGGGC CCCCGGGCCT ACAAGCGCTG GGTGTGCTCC 
CTGCCCTTTG GCCATGTTCA ATTCTCAGAG AAGATTCAGC GCTTCACACC CTTCTCCCTC 
GGCTTTGAGT TCTTACCTGG AGAGACTTAC TACTACATCT CGGTGCCCAC TCCAGAGAGT 
TCTGGCCAGT GCTTGAGGCT CCAGGTGTCT GTCTGCTGCA AGGAGAGGAA GTCTGAGTCA 
GCCCATCCTG TTGGGAGCCC TGGAGAGAGT GGCACATCAG GGTGGCGAGG GGGGGACACT 
CCCAGCCCCC TCTGTCTCTT GCTATTACTG CTGCTTCTGA TTCTTCGTCT TCTGCGAATT 
CTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EFNA4 ( NM_182689.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_872631.1 Homo sapiens ephrin A4 (EFNA4), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu02288
Clone ID Related Accession (Same CDS sequence)	NM_182689.2 , NM_182689.1
Accession Version	NM_182689.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 624bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-27
Organism	Homo sapiens(human)
Product	ephrin-A4 isoform b precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BM740848.1, BE780161.1, AI459151.1 and BQ940212.1. On May 31, 2019 this sequence version replaced NM_182689.1. Transcript Variant: This variant (2) uses an alternate splice site in the 3' coding region, compared to variant 1, that results in a downstream translation termination. It encodes isoform b which has a little longer and distinct C-terminus compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR7346977.158663.1, SRR7346977.61941.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCGGCTGC TGCCCCTGCT GCGGACTGTC CTCTGGGCCG CGTTCCTCGG CTCCCCTCTG 
CGCGGGGGCT CCAGCCTCCG CCACGTAGTC TACTGGAACT CCAGTAACCC CAGGTTGCTT 
CGAGGAGACG CCGTGGTGGA GCTGGGCCTC AACGATTACC TAGACATTGT CTGCCCCCAC 
TACGAAGGCC CAGGGCCCCC TGAGGGCCCC GAGACGTTTG CTTTGTACAT GGTGGACTGG 
CCAGGCTATG AGTCCTGCCA GGCAGAGGGC CCCCGGGCCT ACAAGCGCTG GGTGTGCTCC 
CTGCCCTTTG GCCATGTTCA ATTCTCAGAG AAGATTCAGC GCTTCACACC CTTCTCCCTC 
GGCTTTGAGT TCTTACCTGG AGAGACTTAC TACTACATCT CGGTGCCCAC TCCAGAGAGT 
TCTGGCCAGT GCTTGAGGCT CCAGGTGTCT GTCTGCTGCA AGGAGAGGAG AGCCAGAGTC 
CTCCCAAGAT CCCCTGGAGG AGGAGGGATC CCTGCTGCCT GCACTGGGGG TGCCAATTCA 
GACCGACAAG ATGGAGCATT GATGGGGGAG ATCAGAGGGT CTGAGGTGAC TCTTGCAGGA 
GCCTGTCCCC TCATCACAGG CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_872631.1
CDS	85..708
Translation	MRLLPLLRTVLWAAFLGSPLRGGSSLRHVVYWNSSNPRLLRGDAVVELGLNDYLDIVCPHYEGPGPPEGPETFALYMVDWPGYESCQAEGPRAYKRWVCSLPFGHVQFSEKIQRFTPFSLGFEFLPGETYYYISVPTPESSGQCLRLQVSVCCKERRARVLPRSPGGGGIPAACTGGANSDRQDGALMGEIRGSEVTLAGACPLITG

Target ORF information:

RefSeq Version	NM_182689.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ephrin A4 (EFNA4), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_182689.2

ORF Insert Sequence:

ATGCGGCTGC TGCCCCTGCT GCGGACTGTC CTCTGGGCCG CGTTCCTCGG CTCCCCTCTG 
CGCGGGGGCT CCAGCCTCCG CCACGTAGTC TACTGGAACT CCAGTAACCC CAGGTTGCTT 
CGAGGAGACG CCGTGGTGGA GCTGGGCCTC AACGATTACC TAGACATTGT CTGCCCCCAC 
TACGAAGGCC CAGGGCCCCC TGAGGGCCCC GAGACGTTTG CTTTGTACAT GGTGGACTGG 
CCAGGCTATG AGTCCTGCCA GGCAGAGGGC CCCCGGGCCT ACAAGCGCTG GGTGTGCTCC 
CTGCCCTTTG GCCATGTTCA ATTCTCAGAG AAGATTCAGC GCTTCACACC CTTCTCCCTC 
GGCTTTGAGT TCTTACCTGG AGAGACTTAC TACTACATCT CGGTGCCCAC TCCAGAGAGT 
TCTGGCCAGT GCTTGAGGCT CCAGGTGTCT GTCTGCTGCA AGGAGAGGAG AGCCAGAGTC 
CTCCCAAGAT CCCCTGGAGG AGGAGGGATC CCTGCTGCCT GCACTGGGGG TGCCAATTCA 
GACCGACAAG ATGGAGCATT GATGGGGGAG ATCAGAGGGT CTGAGGTGAC TCTTGCAGGA 
GCCTGTCCCC TCATCACAGG CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EFNA4 ( NM_182689.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_872631.1 Homo sapiens ephrin A4 (EFNA4), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu02288
Clone ID Related Accession (Same CDS sequence)	NM_182689.2 , NM_182689.1
Accession Version	NM_182689.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 624bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-15
Organism	Homo sapiens(human)
Product	ephrin-A4 isoform b precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BM740848.1, BE780161.1, AI459151.1 and BQ940212.1. Transcript Variant: This variant (2) uses an alternate splice site in the 3' coding region, compared to variant 1, that results in a downstream translation termination. It encodes isoform b which has a little longer and distinct C-terminus compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BM740848.1, SRR1803616.42123.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCGGCTGC TGCCCCTGCT GCGGACTGTC CTCTGGGCCG CGTTCCTCGG CTCCCCTCTG 
CGCGGGGGCT CCAGCCTCCG CCACGTAGTC TACTGGAACT CCAGTAACCC CAGGTTGCTT 
CGAGGAGACG CCGTGGTGGA GCTGGGCCTC AACGATTACC TAGACATTGT CTGCCCCCAC 
TACGAAGGCC CAGGGCCCCC TGAGGGCCCC GAGACGTTTG CTTTGTACAT GGTGGACTGG 
CCAGGCTATG AGTCCTGCCA GGCAGAGGGC CCCCGGGCCT ACAAGCGCTG GGTGTGCTCC 
CTGCCCTTTG GCCATGTTCA ATTCTCAGAG AAGATTCAGC GCTTCACACC CTTCTCCCTC 
GGCTTTGAGT TCTTACCTGG AGAGACTTAC TACTACATCT CGGTGCCCAC TCCAGAGAGT 
TCTGGCCAGT GCTTGAGGCT CCAGGTGTCT GTCTGCTGCA AGGAGAGGAG AGCCAGAGTC 
CTCCCAAGAT CCCCTGGAGG AGGAGGGATC CCTGCTGCCT GCACTGGGGG TGCCAATTCA 
GACCGACAAG ATGGAGCATT GATGGGGGAG ATCAGAGGGT CTGAGGTGAC TCTTGCAGGA 
GCCTGTCCCC TCATCACAGG CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_872631.1
CDS	88..711
Translation	MRLLPLLRTVLWAAFLGSPLRGGSSLRHVVYWNSSNPRLLRGDAVVELGLNDYLDIVCPHYEGPGPPEGPETFALYMVDWPGYESCQAEGPRAYKRWVCSLPFGHVQFSEKIQRFTPFSLGFEFLPGETYYYISVPTPESSGQCLRLQVSVCCKERRARVLPRSPGGGGIPAACTGGANSDRQDGALMGEIRGSEVTLAGACPLITG

Target ORF information:

RefSeq Version	NM_182689.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ephrin A4 (EFNA4), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_182689.1

ORF Insert Sequence:

ATGCGGCTGC TGCCCCTGCT GCGGACTGTC CTCTGGGCCG CGTTCCTCGG CTCCCCTCTG 
CGCGGGGGCT CCAGCCTCCG CCACGTAGTC TACTGGAACT CCAGTAACCC CAGGTTGCTT 
CGAGGAGACG CCGTGGTGGA GCTGGGCCTC AACGATTACC TAGACATTGT CTGCCCCCAC 
TACGAAGGCC CAGGGCCCCC TGAGGGCCCC GAGACGTTTG CTTTGTACAT GGTGGACTGG 
CCAGGCTATG AGTCCTGCCA GGCAGAGGGC CCCCGGGCCT ACAAGCGCTG GGTGTGCTCC 
CTGCCCTTTG GCCATGTTCA ATTCTCAGAG AAGATTCAGC GCTTCACACC CTTCTCCCTC 
GGCTTTGAGT TCTTACCTGG AGAGACTTAC TACTACATCT CGGTGCCCAC TCCAGAGAGT 
TCTGGCCAGT GCTTGAGGCT CCAGGTGTCT GTCTGCTGCA AGGAGAGGAG AGCCAGAGTC 
CTCCCAAGAT CCCCTGGAGG AGGAGGGATC CCTGCTGCCT GCACTGGGGG TGCCAATTCA 
GACCGACAAG ATGGAGCATT GATGGGGGAG ATCAGAGGGT CTGAGGTGAC TCTTGCAGGA 
GCCTGTCCCC TCATCACAGG CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EFNA4 ( NM_182690.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_872632.2 Homo sapiens ephrin A4 (EFNA4), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu30425
Clone ID Related Accession (Same CDS sequence)	NM_182690.3 , NM_182690.2
Accession Version	NM_182690.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 582bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-27
Organism	Homo sapiens(human)
Product	ephrin-A4 isoform c precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BM740848.1, AJ006353.1 and AL691442.32. On May 31, 2019 this sequence version replaced NM_182690.2. Transcript Variant: This variant (3), also known as ephrin-A4 (s), uses an alternate splice site in the 3' coding region, compared to variant 1, that results in a downstream translation termination. It encodes isoform c which has a distinct and slightly shorter C-terminus compared to isoform a. Isoform c lacks a characteristic transmembrane domain and the GPI-signal sequence contained in isoform a. Isoform c is a secreted molecule. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AJ006353.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2147596, SAMN01820691 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCGGCTGC TGCCCCTGCT GCGGACTGTC CTCTGGGCCG CGTTCCTCGG CTCCCCTCTG 
CGCGGGGGCT CCAGCCTCCG CCACGTAGTC TACTGGAACT CCAGTAACCC CAGGTTGCTT 
CGAGGAGACG CCGTGGTGGA GCTGGGCCTC AACGATTACC TAGACATTGT CTGCCCCCAC 
TACGAAGGCC CAGGGCCCCC TGAGGGCCCC GAGACGTTTG CTTTGTACAT GGTGGACTGG 
CCAGGCTATG AGTCCTGCCA GGCAGAGGGC CCCCGGGCCT ACAAGCGCTG GGTGTGCTCC 
CTGCCCTTTG GCCATGTTCA ATTCTCAGAG AAGATTCAGC GCTTCACACC CTTCTCCCTC 
GGCTTTGAGT TCTTACCTGG AGAGACTTAC TACTACATCT CGGTGCCCAC TCCAGAGAGT 
TCTGGCCAGT GCTTGAGGCT CCAGGTGTCT GTCTGCTGCA AGGAGAGGAA CCTTCCCTCT 
CATCCCAAGG AGCCAGAGTC CTCCCAAGAT CCCCTGGAGG AGGAGGGATC CCTGCTGCCT 
GCACTGGGGG TGCCAATTCA GACCGACAAG ATGGAGCATT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_872632.2
CDS	85..666
Translation	MRLLPLLRTVLWAAFLGSPLRGGSSLRHVVYWNSSNPRLLRGDAVVELGLNDYLDIVCPHYEGPGPPEGPETFALYMVDWPGYESCQAEGPRAYKRWVCSLPFGHVQFSEKIQRFTPFSLGFEFLPGETYYYISVPTPESSGQCLRLQVSVCCKERNLPSHPKEPESSQDPLEEEGSLLPALGVPIQTDKMEH

Target ORF information:

RefSeq Version	NM_182690.3
Organism	Homo sapiens(human)
Definition	Homo sapiens ephrin A4 (EFNA4), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_182690.3

ORF Insert Sequence:

ATGCGGCTGC TGCCCCTGCT GCGGACTGTC CTCTGGGCCG CGTTCCTCGG CTCCCCTCTG 
CGCGGGGGCT CCAGCCTCCG CCACGTAGTC TACTGGAACT CCAGTAACCC CAGGTTGCTT 
CGAGGAGACG CCGTGGTGGA GCTGGGCCTC AACGATTACC TAGACATTGT CTGCCCCCAC 
TACGAAGGCC CAGGGCCCCC TGAGGGCCCC GAGACGTTTG CTTTGTACAT GGTGGACTGG 
CCAGGCTATG AGTCCTGCCA GGCAGAGGGC CCCCGGGCCT ACAAGCGCTG GGTGTGCTCC 
CTGCCCTTTG GCCATGTTCA ATTCTCAGAG AAGATTCAGC GCTTCACACC CTTCTCCCTC 
GGCTTTGAGT TCTTACCTGG AGAGACTTAC TACTACATCT CGGTGCCCAC TCCAGAGAGT 
TCTGGCCAGT GCTTGAGGCT CCAGGTGTCT GTCTGCTGCA AGGAGAGGAA CCTTCCCTCT 
CATCCCAAGG AGCCAGAGTC CTCCCAAGAT CCCCTGGAGG AGGAGGGATC CCTGCTGCCT 
GCACTGGGGG TGCCAATTCA GACCGACAAG ATGGAGCATT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EFNA4 ( NM_182690.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_872632.2 Homo sapiens ephrin A4 (EFNA4), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu30425
Clone ID Related Accession (Same CDS sequence)	NM_182690.3 , NM_182690.2
Accession Version	NM_182690.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 582bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-22
Organism	Homo sapiens(human)
Product	ephrin-A4 isoform c precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BM740848.1, AJ006353.1 and AL691442.32. On May 25, 2006 this sequence version replaced NM_182690.1. Transcript Variant: This variant (3), also known as ephrin-A4 (s), uses an alternate splice site in the 3' coding region, compared to variant 1, that results in a downstream translation termination. It encodes isoform c which has a distinct and slightly shorter C-terminus compared to isoform a. Isoform c lacks a characteristic transmembrane domain and the GPI-signal sequence contained in isoform a. Isoform c is a secreted molecule. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AJ006353.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2147596, SAMN01820691 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCGGCTGC TGCCCCTGCT GCGGACTGTC CTCTGGGCCG CGTTCCTCGG CTCCCCTCTG 
CGCGGGGGCT CCAGCCTCCG CCACGTAGTC TACTGGAACT CCAGTAACCC CAGGTTGCTT 
CGAGGAGACG CCGTGGTGGA GCTGGGCCTC AACGATTACC TAGACATTGT CTGCCCCCAC 
TACGAAGGCC CAGGGCCCCC TGAGGGCCCC GAGACGTTTG CTTTGTACAT GGTGGACTGG 
CCAGGCTATG AGTCCTGCCA GGCAGAGGGC CCCCGGGCCT ACAAGCGCTG GGTGTGCTCC 
CTGCCCTTTG GCCATGTTCA ATTCTCAGAG AAGATTCAGC GCTTCACACC CTTCTCCCTC 
GGCTTTGAGT TCTTACCTGG AGAGACTTAC TACTACATCT CGGTGCCCAC TCCAGAGAGT 
TCTGGCCAGT GCTTGAGGCT CCAGGTGTCT GTCTGCTGCA AGGAGAGGAA CCTTCCCTCT 
CATCCCAAGG AGCCAGAGTC CTCCCAAGAT CCCCTGGAGG AGGAGGGATC CCTGCTGCCT 
GCACTGGGGG TGCCAATTCA GACCGACAAG ATGGAGCATT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_872632.2
CDS	88..669
Translation	MRLLPLLRTVLWAAFLGSPLRGGSSLRHVVYWNSSNPRLLRGDAVVELGLNDYLDIVCPHYEGPGPPEGPETFALYMVDWPGYESCQAEGPRAYKRWVCSLPFGHVQFSEKIQRFTPFSLGFEFLPGETYYYISVPTPESSGQCLRLQVSVCCKERNLPSHPKEPESSQDPLEEEGSLLPALGVPIQTDKMEH

Target ORF information:

RefSeq Version	NM_182690.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ephrin A4 (EFNA4), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_182690.2

ORF Insert Sequence:

ATGCGGCTGC TGCCCCTGCT GCGGACTGTC CTCTGGGCCG CGTTCCTCGG CTCCCCTCTG 
CGCGGGGGCT CCAGCCTCCG CCACGTAGTC TACTGGAACT CCAGTAACCC CAGGTTGCTT 
CGAGGAGACG CCGTGGTGGA GCTGGGCCTC AACGATTACC TAGACATTGT CTGCCCCCAC 
TACGAAGGCC CAGGGCCCCC TGAGGGCCCC GAGACGTTTG CTTTGTACAT GGTGGACTGG 
CCAGGCTATG AGTCCTGCCA GGCAGAGGGC CCCCGGGCCT ACAAGCGCTG GGTGTGCTCC 
CTGCCCTTTG GCCATGTTCA ATTCTCAGAG AAGATTCAGC GCTTCACACC CTTCTCCCTC 
GGCTTTGAGT TCTTACCTGG AGAGACTTAC TACTACATCT CGGTGCCCAC TCCAGAGAGT 
TCTGGCCAGT GCTTGAGGCT CCAGGTGTCT GTCTGCTGCA AGGAGAGGAA CCTTCCCTCT 
CATCCCAAGG AGCCAGAGTC CTCCCAAGAT CCCCTGGAGG AGGAGGGATC CCTGCTGCCT 
GCACTGGGGG TGCCAATTCA GACCGACAAG ATGGAGCATT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

EphrinA4 plays a critical role in ?4 and ?L mediated survival of human CLL cells during extravasation.
Oncotarget7(30)48481-48500(2016 Jul)
Flores MA,Fortea P,Trinidad EM,Garc?a D,Soler G,Ortu?o FJ,Zapata AG,Alonso-Colmenar LM

Ephrin-A3 and ephrin-A4 contribute to microglia-induced angiogenesis in brain endothelial cells.
Anatomical record (Hoboken, N.J. : 2007)297(10)1908-18(2014 Oct)
Li Y,Liu DX,Li MY,Qin XX,Fang WG,Zhao WD,Chen YH

Ephrin-As, Eph receptors and integrin ?3 interact and colocalise at membrane protrusions of U251MG glioblastoma cells.
Cell biology international37(10)1080-8(2013 Oct)
Makarov A,Ylivinkka I,Nyman TA,Hyyti?inen M,Keski-Oja J

Identification of 23 new prostate cancer susceptibility loci using the iCOGS custom genotyping array.
Nature genetics45(4)385-91, 391e1-2(2013 Apr)
Eeles RA,Olama AA,Benlloch S,Saunders EJ,Leongamornlert DA,Tymrakiewicz M,Ghoussaini M,Luccarini C,Dennis J,Jugurnauth-Little S,Dadaev T,Neal DE,Hamdy FC,Donovan JL,Muir K,Giles GG,Severi G,Wiklund F,Gronberg H,Haiman CA,Schumacher F,Henderson BE,Le Marchand L,Lindstrom S,Kraft P,Hunter DJ,Gapstur S,Chanock SJ,Berndt SI,Albanes D,Andriole G,Schleutker J,Weischer M,Canzian F,Riboli E,Key TJ,Travis RC,Campa D,Ingles SA,John EM,Hayes RB,Pharoah PD,Pashayan N,Khaw KT,Stanford JL,Ostrander EA,Signorello LB,Thibodeau SN,Schaid D,Maier C,Vogel W,Kibel AS,Cybulski C,Lubinski J,Cannon-Albright L,Brenner H,Park JY,Kaneva R,Batra J,Spurdle AB,Clements JA,Teixeira MR,Dicks E,Lee A,Dunning AM,Baynes C,Conroy D,Maranian MJ,Ahmed S,Govindasami K,Guy M,Wilkinson RA,Sawyer EJ,Morgan A,Dearnaley DP,Horwich A,Huddart RA,Khoo VS,Parker CC,Van As NJ,Woodhouse CJ,Thompson A,Dudderidge T,Ogden C,Cooper CS,Lophatananon A,Cox A,Southey MC,Hopper JL,English DR,Aly M,Adolfsson J,Xu J,Zheng SL,Yeager M,Kaaks R,Diver WR,Gaudet MM,Stern MC,Corral R,Joshi AD,Shahabi A,Wahlfors T,Tammela TL,Auvinen A,Virtamo J,Klarskov P,Nordestgaard BG,R?der MA,Nielsen SF,Bojesen SE,Siddiq A,Fitzgerald LM,Kolb S,Kwon EM,Karyadi DM,Blot WJ,Zheng W,Cai Q,McDonnell SK,Rinckleb AE,Drake B,Colditz G,Wokolorczyk D,Stephenson RA,Teerlink C,Muller H,Rothenbacher D,Sellers TA,Lin HY,Slavov C,Mitev V,Lose F,Srinivasan S,Maia S,Paulo P,Lange E,Cooney KA,Antoniou AC,Vincent D,Bacot F,Tessier DC,,,,,,Kote-Jarai Z,Easton DF

Primer-BLAST: a tool to design target-specific primers for polymerase chain reaction.
BMC bioinformatics13134(2012 Jun)
Ye J,Coulouris G,Zaretskaya I,Cutcutache I,Rozen S,Madden TL

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EFNA4 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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