AGO4 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	AGO4
Entrez Gene ID	192670
Full Name	argonaute 4, RISC catalytic component
Synonyms	EIF2C4
General protein information	Preferred Names argonaute 4, RISC catalytic component Names protein argonaute-4 eukaryotic translation initiation factor 2C, 4
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 1 Map Location: 1p34.3
Summary	This gene encodes a member of the Argonaute family of proteins which contain PAZ and PIWI domains and play an integral role in RNA interference and short-interfering-RNA-mediated gene silencing. This gene is located on chromosome 1 in a cluster of related family members. [provided by RefSeq, Mar 2017].
Disorder MIM:	607356

mRNA and Protein(s)

mRNA	Protein	Name
NM_017629.4	NP_060099.2	protein argonaute-4
XM_011540884.2	XP_011539186.1	protein argonaute-4 isoform X3
NM_017629.3	NP_060099.2	protein argonaute-4
XM_011540883.2	XP_011539185.1	protein argonaute-4 isoform X2
XM_024453795.1	XP_024309563.1	protein argonaute-4 isoform X4
XM_017000529.1	XP_016856018.1	protein argonaute-4 isoform X4
XM_005270578.3	XP_005270635.1	protein argonaute-4 isoform X1
XM_005270579.3	XP_005270636.1	protein argonaute-4 isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	EIF2C4	XP_524663.3
Bos taurus (cattle)	AGO4	XP_002686598.2
Danio rerio (zebrafish)	ago4	NP_001239488.1
Arabidopsis thaliana (thale cress)	ZLL	NP_199194.1
Oryza sativa (rice)	Os06g0729300	NP_001058648.1
	EIF2C4	XP_002802359.1
Mus musculus (house mouse)	Ago4	NP_694817.2
Oryza sativa (rice)	Os02g0831600	NP_001048621.1
Homo sapiens (human)	AGO4	NP_060099.2
Gallus gallus (chicken)	AGO4	NP_001034365.1
Canis lupus familiaris (dog)	AGO4	XP_005629018.1
Xenopus tropicalis (tropical clawed frog)	LOC100495218	XP_004911629.1

Gene Ontology
Bibliography

Related articles in PubMed

Generation of catalytic human Ago4 identifies structural elements important for RNA cleavage.
Hauptmann J, Kater L, L?ffler P, Merkl R, Meister G
RNA (New York, N.Y.)20(10)1532-8(2014 Oct)

Molecular dissection of human Argonaute proteins by DNA shuffling.
Sch?rmann N, Trabuco LG, Bender C, Russell RB, Grimm D
Nature structural & molecular biology20(7)818-26(2013 Jul)

Slicing-independent RISC activation requires the argonaute PAZ domain.
Gu S, Jin L, Huang Y, Zhang F, Kay MA
Current biology : CB22(16)1536-42(2012 Aug)

Structural evolution and functional diversification analyses of argonaute protein.
Wei KF, Wu LJ, Chen J, Chen YF, Xie DX
Journal of cellular biochemistry113(8)2576-85(2012 Aug)

Argonaute identity defines the length of mature mammalian microRNAs.
Juvvuna PK, Khandelia P, Lee LM, Makeyev EV
Nucleic acids research40(14)6808-20(2012 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Chimeras with Ago4 N-terminal domains were defective in RISC activation & sometimes also in RNA loading. This highlights the pleiotropic role of the Ago N domain & emphasizes the unique position of human Ago4 within the Ago protein family.
Title: Molecular dissection of human Argonaute proteins by DNA shuffling.

EIF2C2-4 and PIWIL4 appear increased in advanced tumors with distant metastasis, suggesting they may promote tumor invasion
Title: Argonaute proteins: potential biomarkers for human colon cancer.

EIF2C4 protein is expressed in differentiating and terminally differentiated N-type cells more than in S- and I-type cells
Title: Differential expression of Dicer and Argonaute genes during the differentiation of human neuroblastoma cells.

The specificity of RNA interference depends on the concentration of Ago1, Ago3, and Ago4 relative to Ago2.
Title: Importance of translation and nonnucleolytic ago proteins for on-target RNA interference.

The following AGO4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AGO4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu77459	XM_011540884.2 Latest version!	Homo sapiens argonaute RISC component 4 (AGO4), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu83266	XM_017000529.1 Latest version!	Homo sapiens argonaute 4, RISC catalytic component (AGO4), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OHu20382	NM_017629.4 Latest version!	Homo sapiens argonaute RISC component 4 (AGO4), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$265.30 $379.00
OHu33098	XM_005270578.3 Latest version!	Homo sapiens argonaute RISC component 4 (AGO4), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OHu33098	XM_005270579.3 Latest version!	Homo sapiens argonaute RISC component 4 (AGO4), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OHu77458	XM_011540883.2 Latest version!	Homo sapiens argonaute RISC component 4 (AGO4), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OHu103609	XM_024453795.1 Latest version!	Homo sapiens argonaute RISC component 4 (AGO4), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
View Old Accession Versions >>
OHu20382	NM_017629.3	Homo sapiens argonaute 4, RISC catalytic component (AGO4), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$265.30 $379.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

AGO4 ( XM_011540884.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011539186.1 Homo sapiens argonaute RISC component 4 (AGO4), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OHu77459
Clone ID Related Accession (Same CDS sequence)	XM_011540884.2
Accession Version	XM_011540884.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1983bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	protein argonaute-4 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011540884.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAACTC TTATTTCTTC AGTATCTGCA ACTGCTTTCT ACCGGGCTCA GCCTATCATT 
GAGTTCATGT GTGAGGTTTT AGACATTCAG AACATCAATG AACAGACCAA ACCTCTAACA 
GACTCCCAGC GTGTCAAATT TACCAAAGAA ATCAGAGGTC TCAAAGTTGA GGTGACCCAC 
TGTGGACAGA TGAAACGAAA ATACCGAGTT TGTAATGTGA CTAGACGGCC AGCCAGTCAT 
CAAACTTTTC CTTTGCAGCT AGAAAACGGT CAAGCTATGG AATGTACAGT AGCTCAATAT 
TTTAAGCAAA AGTATAGTCT GCAACTGAAA TACCCCCATC TTCCCTGTCT CCAAGTGGGA 
CAAGAACAAA AGCATACATA CTTGCCACTC GAGGTCTGTA ATATAGTGGC AGGACAGCGA 
TGTATCAAGA AGCTCACAGA CAATCAGACT TCCACAATGA TCAAAGCTAC AGCAAGATCT 
GCTCCTGACA GACAGGAAGA GATCAGTAGA CTGGTGAAGA GCAACAGTAT GGTGGGTGGA 
CCTGATCCAT ACCTTAAAGA ATTTGGTATT GTTGTCCACA ATGAAATGAC AGAGCTCACA 
GGCAGGGTAC TTCCAGCACC AATGCTGCAA TATGGAGGCC GGAATAAAAC AGTAGCCACA 
CCCAACCAGG GTGTCTGGGA CATGCGAGGA AAGCAGTTTT ATGCTGGCAT TGAAATTAAA 
GTTTGGGCAG TTGCTTGTTT TGCACCTCAG AAACAATGTA GGGAAGATTT ACTAAAGAGT 
TTCACTGACC AGCTGCGTAA AATCTCTAAG GATGCAGGAA TGCCCATCCA GGGTCAGCCA 
TGTTTCTGCA AGTATGCACA AGGTGCAGAC AGTGTGGAGC CTATGTTTAA ACATCTGAAA 
ATGACTTATG TGGGCCTACA GCTAATAGTG GTTATCCTGC CTGGAAAGAC ACCAGTATAT 
GCGGAGGTGA AACGTGTTGG AGATACCCTT CTAGGTATGG CCACACAGTG TGTCCAGGTA 
AAAAATGTAG TGAAGACCTC ACCTCAAACC CTTTCCAATC TTTGCCTGAA GATAAATGCA 
AAACTTGGAG GAATTAACAA TGTGCTTGTG CCTCATCAAA GGCCCTCGGT GTTCCAGCAG 
CCTGTCATCT TCCTGGGAGC GGATGTCACA CACCCCCCAG CAGGGGATGG GAAGAAACCT 
TCCATTGCTG CTGTGGTTGG CAGTATGGAT GGCCACCCCA GCCGGTACTG TGCCACCGTT 
CGGGTGCAGA CTTCCCGGCA GGAGATCTCC CAAGAGCTCC TCTACAGTCA AGAGGTCATC 
CAGGACCTGA CTAACATGGT TCGAGAGCTG CTGATTCAGT TCTACAAATC CACACGCTTC 
AAACCCACTC GGATCATCTA TTACCGTGGA GGGGTATCTG AGGGACAAAT GAAACAGGTA 
GCTTGGCCAG AACTAATAGC AATTCGAAAG GCATGTATTA GCTTGGAAGA AGATTACCGG 
CCAGGAATAA CTTATATTGT GGTGCAAAAA AGACATCACA CACGACTCTT CTGTGCAGAT 
AAAACAGAAA GGGTAGGGAA AAGTGGCAAT GTACCAGCAG GCACTACAGT GGATAGTACC 
ATCACACATC CATCTGAGTT TGACTTTTAC CTCTGTAGTC ATGCAGGAAT TCAGGGAACC 
AGCCGTCCCT CACATTACCA GGTCTTGTGG GATGACAACT GCTTCACTGC AGATGAACTC 
CAGCTACTGA CTTACCAGCT GTGTCACACC TATGTGAGGT GCACTCGCTC AGTCTCTATT 
CCAGCCCCTG CATATTATGC CCGGCTTGTA GCATTTAGGG CAAGGTATCA TCTGGTGGAT 
AAAGATCATG ACAGTGCGGA AGGCAGTCAT GTGTCAGGAC AGAGCAACGG CCGGGATCCT 
CAGGCCTTGG CTAAGGCTGT GCAAATCCAC CATGATACCC AGCACACGAT GTATTTTGCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011539186.1
CDS	146..2128
Translation	MQTLISSVSATAFYRAQPIIEFMCEVLDIQNINEQTKPLTDSQRVKFTKEIRGLKVEVTHCGQMKRKYRVCNVTRRPASHQTFPLQLENGQAMECTVAQYFKQKYSLQLKYPHLPCLQVGQEQKHTYLPLEVCNIVAGQRCIKKLTDNQTSTMIKATARSAPDRQEEISRLVKSNSMVGGPDPYLKEFGIVVHNEMTELTGRVLPAPMLQYGGRNKTVATPNQGVWDMRGKQFYAGIEIKVWAVACFAPQKQCREDLLKSFTDQLRKISKDAGMPIQGQPCFCKYAQGADSVEPMFKHLKMTYVGLQLIVVILPGKTPVYAEVKRVGDTLLGMATQCVQVKNVVKTSPQTLSNLCLKINAKLGGINNVLVPHQRPSVFQQPVIFLGADVTHPPAGDGKKPSIAAVVGSMDGHPSRYCATVRVQTSRQEISQELLYSQEVIQDLTNMVRELLIQFYKSTRFKPTRIIYYRGGVSEGQMKQVAWPELIAIRKACISLEEDYRPGITYIVVQKRHHTRLFCADKTERVGKSGNVPAGTTVDSTITHPSEFDFYLCSHAGIQGTSRPSHYQVLWDDNCFTADELQLLTYQLCHTYVRCTRSVSIPAPAYYARLVAFRARYHLVDKDHDSAEGSHVSGQSNGRDPQALAKAVQIHHDTQHTMYFA

Target ORF information:

RefSeq Version	XM_011540884.2
Organism	Homo sapiens(human)
Definition	Homo sapiens argonaute RISC component 4 (AGO4), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011540884.2

ORF Insert Sequence:

ATGCAAACTC TTATTTCTTC AGTATCTGCA ACTGCTTTCT ACCGGGCTCA GCCTATCATT 
GAGTTCATGT GTGAGGTTTT AGACATTCAG AACATCAATG AACAGACCAA ACCTCTAACA 
GACTCCCAGC GTGTCAAATT TACCAAAGAA ATCAGAGGTC TCAAAGTTGA GGTGACCCAC 
TGTGGACAGA TGAAACGAAA ATACCGAGTT TGTAATGTGA CTAGACGGCC AGCCAGTCAT 
CAAACTTTTC CTTTGCAGCT AGAAAACGGT CAAGCTATGG AATGTACAGT AGCTCAATAT 
TTTAAGCAAA AGTATAGTCT GCAACTGAAA TACCCCCATC TTCCCTGTCT CCAAGTGGGA 
CAAGAACAAA AGCATACATA CTTGCCACTC GAGGTCTGTA ATATAGTGGC AGGACAGCGA 
TGTATCAAGA AGCTCACAGA CAATCAGACT TCCACAATGA TCAAAGCTAC AGCAAGATCT 
GCTCCTGACA GACAGGAAGA GATCAGTAGA CTGGTGAAGA GCAACAGTAT GGTGGGTGGA 
CCTGATCCAT ACCTTAAAGA ATTTGGTATT GTTGTCCACA ATGAAATGAC AGAGCTCACA 
GGCAGGGTAC TTCCAGCACC AATGCTGCAA TATGGAGGCC GGAATAAAAC AGTAGCCACA 
CCCAACCAGG GTGTCTGGGA CATGCGAGGA AAGCAGTTTT ATGCTGGCAT TGAAATTAAA 
GTTTGGGCAG TTGCTTGTTT TGCACCTCAG AAACAATGTA GGGAAGATTT ACTAAAGAGT 
TTCACTGACC AGCTGCGTAA AATCTCTAAG GATGCAGGAA TGCCCATCCA GGGTCAGCCA 
TGTTTCTGCA AGTATGCACA AGGTGCAGAC AGTGTGGAGC CTATGTTTAA ACATCTGAAA 
ATGACTTATG TGGGCCTACA GCTAATAGTG GTTATCCTGC CTGGAAAGAC ACCAGTATAT 
GCGGAGGTGA AACGTGTTGG AGATACCCTT CTAGGTATGG CCACACAGTG TGTCCAGGTA 
AAAAATGTAG TGAAGACCTC ACCTCAAACC CTTTCCAATC TTTGCCTGAA GATAAATGCA 
AAACTTGGAG GAATTAACAA TGTGCTTGTG CCTCATCAAA GGCCCTCGGT GTTCCAGCAG 
CCTGTCATCT TCCTGGGAGC GGATGTCACA CACCCCCCAG CAGGGGATGG GAAGAAACCT 
TCCATTGCTG CTGTGGTTGG CAGTATGGAT GGCCACCCCA GCCGGTACTG TGCCACCGTT 
CGGGTGCAGA CTTCCCGGCA GGAGATCTCC CAAGAGCTCC TCTACAGTCA AGAGGTCATC 
CAGGACCTGA CTAACATGGT TCGAGAGCTG CTGATTCAGT TCTACAAATC CACACGCTTC 
AAACCCACTC GGATCATCTA TTACCGTGGA GGGGTATCTG AGGGACAAAT GAAACAGGTA 
GCTTGGCCAG AACTAATAGC AATTCGAAAG GCATGTATTA GCTTGGAAGA AGATTACCGG 
CCAGGAATAA CTTATATTGT GGTGCAAAAA AGACATCACA CACGACTCTT CTGTGCAGAT 
AAAACAGAAA GGGTAGGGAA AAGTGGCAAT GTACCAGCAG GCACTACAGT GGATAGTACC 
ATCACACATC CATCTGAGTT TGACTTTTAC CTCTGTAGTC ATGCAGGAAT TCAGGGAACC 
AGCCGTCCCT CACATTACCA GGTCTTGTGG GATGACAACT GCTTCACTGC AGATGAACTC 
CAGCTACTGA CTTACCAGCT GTGTCACACC TATGTGAGGT GCACTCGCTC AGTCTCTATT 
CCAGCCCCTG CATATTATGC CCGGCTTGTA GCATTTAGGG CAAGGTATCA TCTGGTGGAT 
AAAGATCATG ACAGTGCGGA AGGCAGTCAT GTGTCAGGAC AGAGCAACGG CCGGGATCCT 
CAGGCCTTGG CTAAGGCTGT GCAAATCCAC CATGATACCC AGCACACGAT GTATTTTGCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AGO4 ( XM_017000529.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016856018.1 Homo sapiens argonaute 4, RISC catalytic component (AGO4), transcript variant X12, mRNA.

Price & Availability↑

CloneID	OHu83266
Clone ID Related Accession (Same CDS sequence)	XM_017000529.1
Accession Version	XM_017000529.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1137bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	protein argonaute-4 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGCGC TGGGACCCGG ACCTCCGGCT AGCCTGTTTC AGCCACCTCG TCGTCCTGGC 
CTTGGAACTG TTGGAAAACC AATTCGACTG TTAGCCAATC ATTTTCAGGT TCAGATTCCT 
AAAATAGATG TGTATCACTA TGATGTGGAT ATTAAGCCTG AAAAACGGCC TCGTAGAGTC 
AACAGGGAGG TAGTAGATAC AATGGTGCGG CACTTCAAGA TGCAAATATT TGGTGATCGG 
CAGCCTGGGT ATGATGGCAA AAGAAACATG TACACAGCAC ATCCACTACC AATTGGACGG 
GATAGGGTTG ATATGGAGGT GACTCTTCCA GGCGAGGGTA AAGACCAAAC ATTTAAAGTG 
TCTGTTCAGT GGGTGTCAGT TGTGAGCCTT CAGTTGCTTT TAGAAGCTTT GGCTGGGCAC 
TTGAATGAAG TCCCAGATGA CTCAGTACAA GCACTTGATG TTATCACAAG ACACCTTCCC 
TCCATGAGGT ACACCCCAGT GGGCCGTTCC TTTTTCTCAC CCCCGGAAGG TTACTACCAC 
CCTCTGGGAG GGGGCAGGGA GGTCTGGTTT GGTTTTCATC AGTCTGTGAG ACCTGCCATG 
TGGAATATGA TGCTCAACAT TGATGTATCT GCAACTGCTT TCTACCGGGC TCAGCCTATC 
ATTGAGTTCA TGTGTGAGGT TTTAGACATT CAGAACATCA ATGAACAGAC CAAACCTCTA 
ACAGACTCCC AGCGTGTCAA ATTTACCAAA GAAATCAGAG GTCTCAAAGT TGAGGTGACC 
CACTGTGGAC AGATGAAACG AAAATACCGA GTTTGTAATG TGACTAGACG GCCAGCCAGT 
CATCAAACTT TTCCTTTGCA GCTAGAAAAC GGTCAAGCTA TGGAATGTAC AGTAGCTCAA 
TATTTTAAGC AAAAGTATAG TCTGCAACTG AAATACCCCC ATCTTCCCTG TCTCCAAGTG 
GGACAAGAAC AAAAGCATAC ATACTTGCCA CTCGAGGTCT GTAATATAGT GGCAGGACAG 
CGATGTATCA AGAAGCTCAC AGACAATCAG ACTTCCACAA TGATCAAAGC TACAGCAAGA 
TCTGCTCCTG ACAGACAGGA AGAGATCAGT AGACTGGCTT TCCTGTTCTT TAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016856018.1
CDS	88..1224
Translation	MEALGPGPPASLFQPPRRPGLGTVGKPIRLLANHFQVQIPKIDVYHYDVDIKPEKRPRRVNREVVDTMVRHFKMQIFGDRQPGYDGKRNMYTAHPLPIGRDRVDMEVTLPGEGKDQTFKVSVQWVSVVSLQLLLEALAGHLNEVPDDSVQALDVITRHLPSMRYTPVGRSFFSPPEGYYHPLGGGREVWFGFHQSVRPAMWNMMLNIDVSATAFYRAQPIIEFMCEVLDIQNINEQTKPLTDSQRVKFTKEIRGLKVEVTHCGQMKRKYRVCNVTRRPASHQTFPLQLENGQAMECTVAQYFKQKYSLQLKYPHLPCLQVGQEQKHTYLPLEVCNIVAGQRCIKKLTDNQTSTMIKATARSAPDRQEEISRLAFLFFR

Target ORF information:

RefSeq Version	XM_017000529.1
Organism	Homo sapiens(human)
Definition	Homo sapiens argonaute 4, RISC catalytic component (AGO4), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017000529.1

ORF Insert Sequence:

ATGGAGGCGC TGGGACCCGG ACCTCCGGCT AGCCTGTTTC AGCCACCTCG TCGTCCTGGC 
CTTGGAACTG TTGGAAAACC AATTCGACTG TTAGCCAATC ATTTTCAGGT TCAGATTCCT 
AAAATAGATG TGTATCACTA TGATGTGGAT ATTAAGCCTG AAAAACGGCC TCGTAGAGTC 
AACAGGGAGG TAGTAGATAC AATGGTGCGG CACTTCAAGA TGCAAATATT TGGTGATCGG 
CAGCCTGGGT ATGATGGCAA AAGAAACATG TACACAGCAC ATCCACTACC AATTGGACGG 
GATAGGGTTG ATATGGAGGT GACTCTTCCA GGCGAGGGTA AAGACCAAAC ATTTAAAGTG 
TCTGTTCAGT GGGTGTCAGT TGTGAGCCTT CAGTTGCTTT TAGAAGCTTT GGCTGGGCAC 
TTGAATGAAG TCCCAGATGA CTCAGTACAA GCACTTGATG TTATCACAAG ACACCTTCCC 
TCCATGAGGT ACACCCCAGT GGGCCGTTCC TTTTTCTCAC CCCCGGAAGG TTACTACCAC 
CCTCTGGGAG GGGGCAGGGA GGTCTGGTTT GGTTTTCATC AGTCTGTGAG ACCTGCCATG 
TGGAATATGA TGCTCAACAT TGATGTATCT GCAACTGCTT TCTACCGGGC TCAGCCTATC 
ATTGAGTTCA TGTGTGAGGT TTTAGACATT CAGAACATCA ATGAACAGAC CAAACCTCTA 
ACAGACTCCC AGCGTGTCAA ATTTACCAAA GAAATCAGAG GTCTCAAAGT TGAGGTGACC 
CACTGTGGAC AGATGAAACG AAAATACCGA GTTTGTAATG TGACTAGACG GCCAGCCAGT 
CATCAAACTT TTCCTTTGCA GCTAGAAAAC GGTCAAGCTA TGGAATGTAC AGTAGCTCAA 
TATTTTAAGC AAAAGTATAG TCTGCAACTG AAATACCCCC ATCTTCCCTG TCTCCAAGTG 
GGACAAGAAC AAAAGCATAC ATACTTGCCA CTCGAGGTCT GTAATATAGT GGCAGGACAG 
CGATGTATCA AGAAGCTCAC AGACAATCAG ACTTCCACAA TGATCAAAGC TACAGCAAGA 
TCTGCTCCTG ACAGACAGGA AGAGATCAGT AGACTGGCTT TCCTGTTCTT TAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AGO4 ( NM_017629.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_060099.2 Homo sapiens argonaute RISC component 4 (AGO4), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu20382
Clone ID Related Accession (Same CDS sequence)	NM_017629.4 , NM_017629.3
Accession Version	NM_017629.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2586bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-21
Organism	Homo sapiens(human)
Product	protein argonaute-4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL354864.16, AB046787.1, AL359186.15 and CA419947.1. On Nov 22, 2018 this sequence version replaced NM_017629.3. Sequence Note: Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC152450.1, AB046787.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000373210.4/ ENSP00000362306.3 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGAGGCGC TGGGACCCGG ACCTCCGGCT AGCCTGTTTC AGCCACCTCG TCGTCCTGGC 
CTTGGAACTG TTGGAAAACC AATTCGACTG TTAGCCAATC ATTTTCAGGT TCAGATTCCT 
AAAATAGATG TGTATCACTA TGATGTGGAT ATTAAGCCTG AAAAACGGCC TCGTAGAGTC 
AACAGGGAGG TAGTAGATAC AATGGTGCGG CACTTCAAGA TGCAAATATT TGGTGATCGG 
CAGCCTGGGT ATGATGGCAA AAGAAACATG TACACAGCAC ATCCACTACC AATTGGACGG 
GATAGGGTTG ATATGGAGGT GACTCTTCCA GGCGAGGGTA AAGACCAAAC ATTTAAAGTG 
TCTGTTCAGT GGGTGTCAGT TGTGAGCCTT CAGTTGCTTT TAGAAGCTTT GGCTGGGCAC 
TTGAATGAAG TCCCAGATGA CTCAGTACAA GCACTTGATG TTATCACAAG ACACCTTCCC 
TCCATGAGGT ACACCCCAGT GGGCCGTTCC TTTTTCTCAC CCCCGGAAGG TTACTACCAC 
CCTCTGGGAG GGGGCAGGGA GGTCTGGTTT GGTTTTCATC AGTCTGTGAG ACCTGCCATG 
TGGAATATGA TGCTCAACAT TGATGTATCT GCAACTGCTT TCTACCGGGC TCAGCCTATC 
ATTGAGTTCA TGTGTGAGGT TTTAGACATT CAGAACATCA ATGAACAGAC CAAACCTCTA 
ACAGACTCCC AGCGTGTCAA ATTTACCAAA GAAATCAGAG GTCTCAAAGT TGAGGTGACC 
CACTGTGGAC AGATGAAACG AAAATACCGA GTTTGTAATG TGACTAGACG GCCAGCCAGT 
CATCAAACTT TTCCTTTGCA GCTAGAAAAC GGTCAAGCTA TGGAATGTAC AGTAGCTCAA 
TATTTTAAGC AAAAGTATAG TCTGCAACTG AAATACCCCC ATCTTCCCTG TCTCCAAGTG 
GGACAAGAAC AAAAGCATAC ATACTTGCCA CTCGAGGTCT GTAATATAGT GGCAGGACAG 
CGATGTATCA AGAAGCTCAC AGACAATCAG ACTTCCACAA TGATCAAAGC TACAGCAAGA 
TCTGCTCCTG ACAGACAGGA AGAGATCAGT AGACTGGTGA AGAGCAACAG TATGGTGGGT 
GGACCTGATC CATACCTTAA AGAATTTGGT ATTGTTGTCC ACAATGAAAT GACAGAGCTC 
ACAGGCAGGG TACTTCCAGC ACCAATGCTG CAATATGGAG GCCGGAATAA AACAGTAGCC 
ACACCCAACC AGGGTGTCTG GGACATGCGA GGAAAGCAGT TTTATGCTGG CATTGAAATT 
AAAGTTTGGG CAGTTGCTTG TTTTGCACCT CAGAAACAAT GTAGGGAAGA TTTACTAAAG 
AGTTTCACTG ACCAGCTGCG TAAAATCTCT AAGGATGCAG GAATGCCCAT CCAGGGTCAG 
CCATGTTTCT GCAAGTATGC ACAAGGTGCA GACAGTGTGG AGCCTATGTT TAAACATCTG 
AAAATGACTT ATGTGGGCCT ACAGCTAATA GTGGTTATCC TGCCTGGAAA GACACCAGTA 
TATGCGGAGG TGAAACGTGT TGGAGATACC CTTCTAGGTA TGGCCACACA GTGTGTCCAG 
GTAAAAAATG TAGTGAAGAC CTCACCTCAA ACCCTTTCCA ATCTTTGCCT GAAGATAAAT 
GCAAAACTTG GAGGAATTAA CAATGTGCTT GTGCCTCATC AAAGGCCCTC GGTGTTCCAG 
CAGCCTGTCA TCTTCCTGGG AGCGGATGTC ACACACCCCC CAGCAGGGGA TGGGAAGAAA 
CCTTCCATTG CTGCTGTGGT TGGCAGTATG GATGGCCACC CCAGCCGGTA CTGTGCCACC 
GTTCGGGTGC AGACTTCCCG GCAGGAGATC TCCCAAGAGC TCCTCTACAG TCAAGAGGTC 
ATCCAGGACC TGACTAACAT GGTTCGAGAG CTGCTGATTC AGTTCTACAA ATCCACACGC 
TTCAAACCCA CTCGGATCAT CTATTACCGT GGAGGGGTAT CTGAGGGACA AATGAAACAG 
GTAGCTTGGC CAGAACTAAT AGCAATTCGA AAGGCATGTA TTAGCTTGGA AGAAGATTAC 
CGGCCAGGAA TAACTTATAT TGTGGTGCAA AAAAGACATC ACACACGACT CTTCTGTGCA 
GATAAAACAG AAAGGGTAGG GAAAAGTGGC AATGTACCAG CAGGCACTAC AGTGGATAGT 
ACCATCACAC ATCCATCTGA GTTTGACTTT TACCTCTGTA GTCATGCAGG AATTCAGGGA 
ACCAGCCGTC CCTCACATTA CCAGGTCTTG TGGGATGACA ACTGCTTCAC TGCAGATGAA 
CTCCAGCTAC TGACTTACCA GCTGTGTCAC ACCTATGTGA GGTGCACTCG CTCAGTCTCT 
ATTCCAGCCC CTGCATATTA TGCCCGGCTT GTAGCATTTA GGGCAAGGTA TCATCTGGTG 
GATAAAGATC ATGACAGTGC GGAAGGCAGT CATGTGTCAG GACAGAGCAA CGGCCGGGAT 
CCTCAGGCCT TGGCTAAGGC TGTGCAAATC CACCATGATA CCCAGCACAC GATGTATTTT 
GCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_060099.2
CDS	402..2987
Translation	MEALGPGPPASLFQPPRRPGLGTVGKPIRLLANHFQVQIPKIDVYHYDVDIKPEKRPRRVNREVVDTMVRHFKMQIFGDRQPGYDGKRNMYTAHPLPIGRDRVDMEVTLPGEGKDQTFKVSVQWVSVVSLQLLLEALAGHLNEVPDDSVQALDVITRHLPSMRYTPVGRSFFSPPEGYYHPLGGGREVWFGFHQSVRPAMWNMMLNIDVSATAFYRAQPIIEFMCEVLDIQNINEQTKPLTDSQRVKFTKEIRGLKVEVTHCGQMKRKYRVCNVTRRPASHQTFPLQLENGQAMECTVAQYFKQKYSLQLKYPHLPCLQVGQEQKHTYLPLEVCNIVAGQRCIKKLTDNQTSTMIKATARSAPDRQEEISRLVKSNSMVGGPDPYLKEFGIVVHNEMTELTGRVLPAPMLQYGGRNKTVATPNQGVWDMRGKQFYAGIEIKVWAVACFAPQKQCREDLLKSFTDQLRKISKDAGMPIQGQPCFCKYAQGADSVEPMFKHLKMTYVGLQLIVVILPGKTPVYAEVKRVGDTLLGMATQCVQVKNVVKTSPQTLSNLCLKINAKLGGINNVLVPHQRPSVFQQPVIFLGADVTHPPAGDGKKPSIAAVVGSMDGHPSRYCATVRVQTSRQEISQELLYSQEVIQDLTNMVRELLIQFYKSTRFKPTRIIYYRGGVSEGQMKQVAWPELIAIRKACISLEEDYRPGITYIVVQKRHHTRLFCADKTERVGKSGNVPAGTTVDSTITHPSEFDFYLCSHAGIQGTSRPSHYQVLWDDNCFTADELQLLTYQLCHTYVRCTRSVSIPAPAYYARLVAFRARYHLVDKDHDSAEGSHVSGQSNGRDPQALAKAVQIHHDTQHTMYFA

Target ORF information:

RefSeq Version	NM_017629.4
Organism	Homo sapiens(human)
Definition	Homo sapiens argonaute RISC component 4 (AGO4), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_017629.4

ORF Insert Sequence:

ATGGAGGCGC TGGGACCCGG ACCTCCGGCT AGCCTGTTTC AGCCACCTCG TCGTCCTGGC 
CTTGGAACTG TTGGAAAACC AATTCGACTG TTAGCCAATC ATTTTCAGGT TCAGATTCCT 
AAAATAGATG TGTATCACTA TGATGTGGAT ATTAAGCCTG AAAAACGGCC TCGTAGAGTC 
AACAGGGAGG TAGTAGATAC AATGGTGCGG CACTTCAAGA TGCAAATATT TGGTGATCGG 
CAGCCTGGGT ATGATGGCAA AAGAAACATG TACACAGCAC ATCCACTACC AATTGGACGG 
GATAGGGTTG ATATGGAGGT GACTCTTCCA GGCGAGGGTA AAGACCAAAC ATTTAAAGTG 
TCTGTTCAGT GGGTGTCAGT TGTGAGCCTT CAGTTGCTTT TAGAAGCTTT GGCTGGGCAC 
TTGAATGAAG TCCCAGATGA CTCAGTACAA GCACTTGATG TTATCACAAG ACACCTTCCC 
TCCATGAGGT ACACCCCAGT GGGCCGTTCC TTTTTCTCAC CCCCGGAAGG TTACTACCAC 
CCTCTGGGAG GGGGCAGGGA GGTCTGGTTT GGTTTTCATC AGTCTGTGAG ACCTGCCATG 
TGGAATATGA TGCTCAACAT TGATGTATCT GCAACTGCTT TCTACCGGGC TCAGCCTATC 
ATTGAGTTCA TGTGTGAGGT TTTAGACATT CAGAACATCA ATGAACAGAC CAAACCTCTA 
ACAGACTCCC AGCGTGTCAA ATTTACCAAA GAAATCAGAG GTCTCAAAGT TGAGGTGACC 
CACTGTGGAC AGATGAAACG AAAATACCGA GTTTGTAATG TGACTAGACG GCCAGCCAGT 
CATCAAACTT TTCCTTTGCA GCTAGAAAAC GGTCAAGCTA TGGAATGTAC AGTAGCTCAA 
TATTTTAAGC AAAAGTATAG TCTGCAACTG AAATACCCCC ATCTTCCCTG TCTCCAAGTG 
GGACAAGAAC AAAAGCATAC ATACTTGCCA CTCGAGGTCT GTAATATAGT GGCAGGACAG 
CGATGTATCA AGAAGCTCAC AGACAATCAG ACTTCCACAA TGATCAAAGC TACAGCAAGA 
TCTGCTCCTG ACAGACAGGA AGAGATCAGT AGACTGGTGA AGAGCAACAG TATGGTGGGT 
GGACCTGATC CATACCTTAA AGAATTTGGT ATTGTTGTCC ACAATGAAAT GACAGAGCTC 
ACAGGCAGGG TACTTCCAGC ACCAATGCTG CAATATGGAG GCCGGAATAA AACAGTAGCC 
ACACCCAACC AGGGTGTCTG GGACATGCGA GGAAAGCAGT TTTATGCTGG CATTGAAATT 
AAAGTTTGGG CAGTTGCTTG TTTTGCACCT CAGAAACAAT GTAGGGAAGA TTTACTAAAG 
AGTTTCACTG ACCAGCTGCG TAAAATCTCT AAGGATGCAG GAATGCCCAT CCAGGGTCAG 
CCATGTTTCT GCAAGTATGC ACAAGGTGCA GACAGTGTGG AGCCTATGTT TAAACATCTG 
AAAATGACTT ATGTGGGCCT ACAGCTAATA GTGGTTATCC TGCCTGGAAA GACACCAGTA 
TATGCGGAGG TGAAACGTGT TGGAGATACC CTTCTAGGTA TGGCCACACA GTGTGTCCAG 
GTAAAAAATG TAGTGAAGAC CTCACCTCAA ACCCTTTCCA ATCTTTGCCT GAAGATAAAT 
GCAAAACTTG GAGGAATTAA CAATGTGCTT GTGCCTCATC AAAGGCCCTC GGTGTTCCAG 
CAGCCTGTCA TCTTCCTGGG AGCGGATGTC ACACACCCCC CAGCAGGGGA TGGGAAGAAA 
CCTTCCATTG CTGCTGTGGT TGGCAGTATG GATGGCCACC CCAGCCGGTA CTGTGCCACC 
GTTCGGGTGC AGACTTCCCG GCAGGAGATC TCCCAAGAGC TCCTCTACAG TCAAGAGGTC 
ATCCAGGACC TGACTAACAT GGTTCGAGAG CTGCTGATTC AGTTCTACAA ATCCACACGC 
TTCAAACCCA CTCGGATCAT CTATTACCGT GGAGGGGTAT CTGAGGGACA AATGAAACAG 
GTAGCTTGGC CAGAACTAAT AGCAATTCGA AAGGCATGTA TTAGCTTGGA AGAAGATTAC 
CGGCCAGGAA TAACTTATAT TGTGGTGCAA AAAAGACATC ACACACGACT CTTCTGTGCA 
GATAAAACAG AAAGGGTAGG GAAAAGTGGC AATGTACCAG CAGGCACTAC AGTGGATAGT 
ACCATCACAC ATCCATCTGA GTTTGACTTT TACCTCTGTA GTCATGCAGG AATTCAGGGA 
ACCAGCCGTC CCTCACATTA CCAGGTCTTG TGGGATGACA ACTGCTTCAC TGCAGATGAA 
CTCCAGCTAC TGACTTACCA GCTGTGTCAC ACCTATGTGA GGTGCACTCG CTCAGTCTCT 
ATTCCAGCCC CTGCATATTA TGCCCGGCTT GTAGCATTTA GGGCAAGGTA TCATCTGGTG 
GATAAAGATC ATGACAGTGC GGAAGGCAGT CATGTGTCAG GACAGAGCAA CGGCCGGGAT 
CCTCAGGCCT TGGCTAAGGC TGTGCAAATC CACCATGATA CCCAGCACAC GATGTATTTT 
GCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AGO4 ( NM_017629.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_060099.2 Homo sapiens argonaute 4, RISC catalytic component (AGO4), transcript variant 1, mRNA.

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CloneID	OHu20382
Clone ID Related Accession (Same CDS sequence)	NM_017629.4 , NM_017629.3
Accession Version	NM_017629.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2586bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	protein argonaute-4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AB046787.1, AL359186.15 and CA419947.1. On Feb 25, 2011 this sequence version replaced NM_017629.2. Sequence Note: Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC152450.1, AB046787.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGGCGC TGGGACCCGG ACCTCCGGCT AGCCTGTTTC AGCCACCTCG TCGTCCTGGC 
CTTGGAACTG TTGGAAAACC AATTCGACTG TTAGCCAATC ATTTTCAGGT TCAGATTCCT 
AAAATAGATG TGTATCACTA TGATGTGGAT ATTAAGCCTG AAAAACGGCC TCGTAGAGTC 
AACAGGGAGG TAGTAGATAC AATGGTGCGG CACTTCAAGA TGCAAATATT TGGTGATCGG 
CAGCCTGGGT ATGATGGCAA AAGAAACATG TACACAGCAC ATCCACTACC AATTGGACGG 
GATAGGGTTG ATATGGAGGT GACTCTTCCA GGCGAGGGTA AAGACCAAAC ATTTAAAGTG 
TCTGTTCAGT GGGTGTCAGT TGTGAGCCTT CAGTTGCTTT TAGAAGCTTT GGCTGGGCAC 
TTGAATGAAG TCCCAGATGA CTCAGTACAA GCACTTGATG TTATCACAAG ACACCTTCCC 
TCCATGAGGT ACACCCCAGT GGGCCGTTCC TTTTTCTCAC CCCCGGAAGG TTACTACCAC 
CCTCTGGGAG GGGGCAGGGA GGTCTGGTTT GGTTTTCATC AGTCTGTGAG ACCTGCCATG 
TGGAATATGA TGCTCAACAT TGATGTATCT GCAACTGCTT TCTACCGGGC TCAGCCTATC 
ATTGAGTTCA TGTGTGAGGT TTTAGACATT CAGAACATCA ATGAACAGAC CAAACCTCTA 
ACAGACTCCC AGCGTGTCAA ATTTACCAAA GAAATCAGAG GTCTCAAAGT TGAGGTGACC 
CACTGTGGAC AGATGAAACG AAAATACCGA GTTTGTAATG TGACTAGACG GCCAGCCAGT 
CATCAAACTT TTCCTTTGCA GCTAGAAAAC GGTCAAGCTA TGGAATGTAC AGTAGCTCAA 
TATTTTAAGC AAAAGTATAG TCTGCAACTG AAATACCCCC ATCTTCCCTG TCTCCAAGTG 
GGACAAGAAC AAAAGCATAC ATACTTGCCA CTCGAGGTCT GTAATATAGT GGCAGGACAG 
CGATGTATCA AGAAGCTCAC AGACAATCAG ACTTCCACAA TGATCAAAGC TACAGCAAGA 
TCTGCTCCTG ACAGACAGGA AGAGATCAGT AGACTGGTGA AGAGCAACAG TATGGTGGGT 
GGACCTGATC CATACCTTAA AGAATTTGGT ATTGTTGTCC ACAATGAAAT GACAGAGCTC 
ACAGGCAGGG TACTTCCAGC ACCAATGCTG CAATATGGAG GCCGGAATAA AACAGTAGCC 
ACACCCAACC AGGGTGTCTG GGACATGCGA GGAAAGCAGT TTTATGCTGG CATTGAAATT 
AAAGTTTGGG CAGTTGCTTG TTTTGCACCT CAGAAACAAT GTAGGGAAGA TTTACTAAAG 
AGTTTCACTG ACCAGCTGCG TAAAATCTCT AAGGATGCAG GAATGCCCAT CCAGGGTCAG 
CCATGTTTCT GCAAGTATGC ACAAGGTGCA GACAGTGTGG AGCCTATGTT TAAACATCTG 
AAAATGACTT ATGTGGGCCT ACAGCTAATA GTGGTTATCC TGCCTGGAAA GACACCAGTA 
TATGCGGAGG TGAAACGTGT TGGAGATACC CTTCTAGGTA TGGCCACACA GTGTGTCCAG 
GTAAAAAATG TAGTGAAGAC CTCACCTCAA ACCCTTTCCA ATCTTTGCCT GAAGATAAAT 
GCAAAACTTG GAGGAATTAA CAATGTGCTT GTGCCTCATC AAAGGCCCTC GGTGTTCCAG 
CAGCCTGTCA TCTTCCTGGG AGCGGATGTC ACACACCCCC CAGCAGGGGA TGGGAAGAAA 
CCTTCCATTG CTGCTGTGGT TGGCAGTATG GATGGCCACC CCAGCCGGTA CTGTGCCACC 
GTTCGGGTGC AGACTTCCCG GCAGGAGATC TCCCAAGAGC TCCTCTACAG TCAAGAGGTC 
ATCCAGGACC TGACTAACAT GGTTCGAGAG CTGCTGATTC AGTTCTACAA ATCCACACGC 
TTCAAACCCA CTCGGATCAT CTATTACCGT GGAGGGGTAT CTGAGGGACA AATGAAACAG 
GTAGCTTGGC CAGAACTAAT AGCAATTCGA AAGGCATGTA TTAGCTTGGA AGAAGATTAC 
CGGCCAGGAA TAACTTATAT TGTGGTGCAA AAAAGACATC ACACACGACT CTTCTGTGCA 
GATAAAACAG AAAGGGTAGG GAAAAGTGGC AATGTACCAG CAGGCACTAC AGTGGATAGT 
ACCATCACAC ATCCATCTGA GTTTGACTTT TACCTCTGTA GTCATGCAGG AATTCAGGGA 
ACCAGCCGTC CCTCACATTA CCAGGTCTTG TGGGATGACA ACTGCTTCAC TGCAGATGAA 
CTCCAGCTAC TGACTTACCA GCTGTGTCAC ACCTATGTGA GGTGCACTCG CTCAGTCTCT 
ATTCCAGCCC CTGCATATTA TGCCCGGCTT GTAGCATTTA GGGCAAGGTA TCATCTGGTG 
GATAAAGATC ATGACAGTGC GGAAGGCAGT CATGTGTCAG GACAGAGCAA CGGCCGGGAT 
CCTCAGGCCT TGGCTAAGGC TGTGCAAATC CACCATGATA CCCAGCACAC GATGTATTTT 
GCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_060099.2
CDS	191..2776
Translation	MEALGPGPPASLFQPPRRPGLGTVGKPIRLLANHFQVQIPKIDVYHYDVDIKPEKRPRRVNREVVDTMVRHFKMQIFGDRQPGYDGKRNMYTAHPLPIGRDRVDMEVTLPGEGKDQTFKVSVQWVSVVSLQLLLEALAGHLNEVPDDSVQALDVITRHLPSMRYTPVGRSFFSPPEGYYHPLGGGREVWFGFHQSVRPAMWNMMLNIDVSATAFYRAQPIIEFMCEVLDIQNINEQTKPLTDSQRVKFTKEIRGLKVEVTHCGQMKRKYRVCNVTRRPASHQTFPLQLENGQAMECTVAQYFKQKYSLQLKYPHLPCLQVGQEQKHTYLPLEVCNIVAGQRCIKKLTDNQTSTMIKATARSAPDRQEEISRLVKSNSMVGGPDPYLKEFGIVVHNEMTELTGRVLPAPMLQYGGRNKTVATPNQGVWDMRGKQFYAGIEIKVWAVACFAPQKQCREDLLKSFTDQLRKISKDAGMPIQGQPCFCKYAQGADSVEPMFKHLKMTYVGLQLIVVILPGKTPVYAEVKRVGDTLLGMATQCVQVKNVVKTSPQTLSNLCLKINAKLGGINNVLVPHQRPSVFQQPVIFLGADVTHPPAGDGKKPSIAAVVGSMDGHPSRYCATVRVQTSRQEISQELLYSQEVIQDLTNMVRELLIQFYKSTRFKPTRIIYYRGGVSEGQMKQVAWPELIAIRKACISLEEDYRPGITYIVVQKRHHTRLFCADKTERVGKSGNVPAGTTVDSTITHPSEFDFYLCSHAGIQGTSRPSHYQVLWDDNCFTADELQLLTYQLCHTYVRCTRSVSIPAPAYYARLVAFRARYHLVDKDHDSAEGSHVSGQSNGRDPQALAKAVQIHHDTQHTMYFA

Target ORF information:

RefSeq Version	NM_017629.3
Organism	Homo sapiens(human)
Definition	Homo sapiens argonaute 4, RISC catalytic component (AGO4), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_017629.3

ORF Insert Sequence:

ATGGAGGCGC TGGGACCCGG ACCTCCGGCT AGCCTGTTTC AGCCACCTCG TCGTCCTGGC 
CTTGGAACTG TTGGAAAACC AATTCGACTG TTAGCCAATC ATTTTCAGGT TCAGATTCCT 
AAAATAGATG TGTATCACTA TGATGTGGAT ATTAAGCCTG AAAAACGGCC TCGTAGAGTC 
AACAGGGAGG TAGTAGATAC AATGGTGCGG CACTTCAAGA TGCAAATATT TGGTGATCGG 
CAGCCTGGGT ATGATGGCAA AAGAAACATG TACACAGCAC ATCCACTACC AATTGGACGG 
GATAGGGTTG ATATGGAGGT GACTCTTCCA GGCGAGGGTA AAGACCAAAC ATTTAAAGTG 
TCTGTTCAGT GGGTGTCAGT TGTGAGCCTT CAGTTGCTTT TAGAAGCTTT GGCTGGGCAC 
TTGAATGAAG TCCCAGATGA CTCAGTACAA GCACTTGATG TTATCACAAG ACACCTTCCC 
TCCATGAGGT ACACCCCAGT GGGCCGTTCC TTTTTCTCAC CCCCGGAAGG TTACTACCAC 
CCTCTGGGAG GGGGCAGGGA GGTCTGGTTT GGTTTTCATC AGTCTGTGAG ACCTGCCATG 
TGGAATATGA TGCTCAACAT TGATGTATCT GCAACTGCTT TCTACCGGGC TCAGCCTATC 
ATTGAGTTCA TGTGTGAGGT TTTAGACATT CAGAACATCA ATGAACAGAC CAAACCTCTA 
ACAGACTCCC AGCGTGTCAA ATTTACCAAA GAAATCAGAG GTCTCAAAGT TGAGGTGACC 
CACTGTGGAC AGATGAAACG AAAATACCGA GTTTGTAATG TGACTAGACG GCCAGCCAGT 
CATCAAACTT TTCCTTTGCA GCTAGAAAAC GGTCAAGCTA TGGAATGTAC AGTAGCTCAA 
TATTTTAAGC AAAAGTATAG TCTGCAACTG AAATACCCCC ATCTTCCCTG TCTCCAAGTG 
GGACAAGAAC AAAAGCATAC ATACTTGCCA CTCGAGGTCT GTAATATAGT GGCAGGACAG 
CGATGTATCA AGAAGCTCAC AGACAATCAG ACTTCCACAA TGATCAAAGC TACAGCAAGA 
TCTGCTCCTG ACAGACAGGA AGAGATCAGT AGACTGGTGA AGAGCAACAG TATGGTGGGT 
GGACCTGATC CATACCTTAA AGAATTTGGT ATTGTTGTCC ACAATGAAAT GACAGAGCTC 
ACAGGCAGGG TACTTCCAGC ACCAATGCTG CAATATGGAG GCCGGAATAA AACAGTAGCC 
ACACCCAACC AGGGTGTCTG GGACATGCGA GGAAAGCAGT TTTATGCTGG CATTGAAATT 
AAAGTTTGGG CAGTTGCTTG TTTTGCACCT CAGAAACAAT GTAGGGAAGA TTTACTAAAG 
AGTTTCACTG ACCAGCTGCG TAAAATCTCT AAGGATGCAG GAATGCCCAT CCAGGGTCAG 
CCATGTTTCT GCAAGTATGC ACAAGGTGCA GACAGTGTGG AGCCTATGTT TAAACATCTG 
AAAATGACTT ATGTGGGCCT ACAGCTAATA GTGGTTATCC TGCCTGGAAA GACACCAGTA 
TATGCGGAGG TGAAACGTGT TGGAGATACC CTTCTAGGTA TGGCCACACA GTGTGTCCAG 
GTAAAAAATG TAGTGAAGAC CTCACCTCAA ACCCTTTCCA ATCTTTGCCT GAAGATAAAT 
GCAAAACTTG GAGGAATTAA CAATGTGCTT GTGCCTCATC AAAGGCCCTC GGTGTTCCAG 
CAGCCTGTCA TCTTCCTGGG AGCGGATGTC ACACACCCCC CAGCAGGGGA TGGGAAGAAA 
CCTTCCATTG CTGCTGTGGT TGGCAGTATG GATGGCCACC CCAGCCGGTA CTGTGCCACC 
GTTCGGGTGC AGACTTCCCG GCAGGAGATC TCCCAAGAGC TCCTCTACAG TCAAGAGGTC 
ATCCAGGACC TGACTAACAT GGTTCGAGAG CTGCTGATTC AGTTCTACAA ATCCACACGC 
TTCAAACCCA CTCGGATCAT CTATTACCGT GGAGGGGTAT CTGAGGGACA AATGAAACAG 
GTAGCTTGGC CAGAACTAAT AGCAATTCGA AAGGCATGTA TTAGCTTGGA AGAAGATTAC 
CGGCCAGGAA TAACTTATAT TGTGGTGCAA AAAAGACATC ACACACGACT CTTCTGTGCA 
GATAAAACAG AAAGGGTAGG GAAAAGTGGC AATGTACCAG CAGGCACTAC AGTGGATAGT 
ACCATCACAC ATCCATCTGA GTTTGACTTT TACCTCTGTA GTCATGCAGG AATTCAGGGA 
ACCAGCCGTC CCTCACATTA CCAGGTCTTG TGGGATGACA ACTGCTTCAC TGCAGATGAA 
CTCCAGCTAC TGACTTACCA GCTGTGTCAC ACCTATGTGA GGTGCACTCG CTCAGTCTCT 
ATTCCAGCCC CTGCATATTA TGCCCGGCTT GTAGCATTTA GGGCAAGGTA TCATCTGGTG 
GATAAAGATC ATGACAGTGC GGAAGGCAGT CATGTGTCAG GACAGAGCAA CGGCCGGGAT 
CCTCAGGCCT TGGCTAAGGC TGTGCAAATC CACCATGATA CCCAGCACAC GATGTATTTT 
GCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AGO4 ( XM_005270578.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_005270635.1 Homo sapiens argonaute RISC component 4 (AGO4), transcript variant X4, mRNA.

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CloneID	OHu33098
Clone ID Related Accession (Same CDS sequence)	XM_005270578.3 , XM_005270579.3
Accession Version	XM_005270578.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2385bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	protein argonaute-4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005270578.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCGGC ACTTCAAGAT GCAAATATTT GGTGATCGGC AGCCTGGGTA TGATGGCAAA 
AGAAACATGT ACACAGCACA TCCACTACCA ATTGGACGGG ATAGGGTTGA TATGGAGGTG 
ACTCTTCCAG GCGAGGGTAA AGACCAAACA TTTAAAGTGT CTGTTCAGTG GGTGTCAGTT 
GTGAGCCTTC AGTTGCTTTT AGAAGCTTTG GCTGGGCACT TGAATGAAGT CCCAGATGAC 
TCAGTACAAG CACTTGATGT TATCACAAGA CACCTTCCCT CCATGAGGTA CACCCCAGTG 
GGCCGTTCCT TTTTCTCACC CCCGGAAGGT TACTACCACC CTCTGGGAGG GGGCAGGGAG 
GTCTGGTTTG GTTTTCATCA GTCTGTGAGA CCTGCCATGT GGAATATGAT GCTCAACATT 
GATGTATCTG CAACTGCTTT CTACCGGGCT CAGCCTATCA TTGAGTTCAT GTGTGAGGTT 
TTAGACATTC AGAACATCAA TGAACAGACC AAACCTCTAA CAGACTCCCA GCGTGTCAAA 
TTTACCAAAG AAATCAGAGG TCTCAAAGTT GAGGTGACCC ACTGTGGACA GATGAAACGA 
AAATACCGAG TTTGTAATGT GACTAGACGG CCAGCCAGTC ATCAAACTTT TCCTTTGCAG 
CTAGAAAACG GTCAAGCTAT GGAATGTACA GTAGCTCAAT ATTTTAAGCA AAAGTATAGT 
CTGCAACTGA AATACCCCCA TCTTCCCTGT CTCCAAGTGG GACAAGAACA AAAGCATACA 
TACTTGCCAC TCGAGGTCTG TAATATAGTG GCAGGACAGC GATGTATCAA GAAGCTCACA 
GACAATCAGA CTTCCACAAT GATCAAAGCT ACAGCAAGAT CTGCTCCTGA CAGACAGGAA 
GAGATCAGTA GACTGGTGAA GAGCAACAGT ATGGTGGGTG GACCTGATCC ATACCTTAAA 
GAATTTGGTA TTGTTGTCCA CAATGAAATG ACAGAGCTCA CAGGCAGGGT ACTTCCAGCA 
CCAATGCTGC AATATGGAGG CCGGAATAAA ACAGTAGCCA CACCCAACCA GGGTGTCTGG 
GACATGCGAG GAAAGCAGTT TTATGCTGGC ATTGAAATTA AAGTTTGGGC AGTTGCTTGT 
TTTGCACCTC AGAAACAATG TAGGGAAGAT TTACTAAAGA GTTTCACTGA CCAGCTGCGT 
AAAATCTCTA AGGATGCAGG AATGCCCATC CAGGGTCAGC CATGTTTCTG CAAGTATGCA 
CAAGGTGCAG ACAGTGTGGA GCCTATGTTT AAACATCTGA AAATGACTTA TGTGGGCCTA 
CAGCTAATAG TGGTTATCCT GCCTGGAAAG ACACCAGTAT ATGCGGAGGT GAAACGTGTT 
GGAGATACCC TTCTAGGTAT GGCCACACAG TGTGTCCAGG TAAAAAATGT AGTGAAGACC 
TCACCTCAAA CCCTTTCCAA TCTTTGCCTG AAGATAAATG CAAAACTTGG AGGAATTAAC 
AATGTGCTTG TGCCTCATCA AAGGCCCTCG GTGTTCCAGC AGCCTGTCAT CTTCCTGGGA 
GCGGATGTCA CACACCCCCC AGCAGGGGAT GGGAAGAAAC CTTCCATTGC TGCTGTGGTT 
GGCAGTATGG ATGGCCACCC CAGCCGGTAC TGTGCCACCG TTCGGGTGCA GACTTCCCGG 
CAGGAGATCT CCCAAGAGCT CCTCTACAGT CAAGAGGTCA TCCAGGACCT GACTAACATG 
GTTCGAGAGC TGCTGATTCA GTTCTACAAA TCCACACGCT TCAAACCCAC TCGGATCATC 
TATTACCGTG GAGGGGTATC TGAGGGACAA ATGAAACAGG TAGCTTGGCC AGAACTAATA 
GCAATTCGAA AGGCATGTAT TAGCTTGGAA GAAGATTACC GGCCAGGAAT AACTTATATT 
GTGGTGCAAA AAAGACATCA CACACGACTC TTCTGTGCAG ATAAAACAGA AAGGGTAGGG 
AAAAGTGGCA ATGTACCAGC AGGCACTACA GTGGATAGTA CCATCACACA TCCATCTGAG 
TTTGACTTTT ACCTCTGTAG TCATGCAGGA ATTCAGGGAA CCAGCCGTCC CTCACATTAC 
CAGGTCTTGT GGGATGACAA CTGCTTCACT GCAGATGAAC TCCAGCTACT GACTTACCAG 
CTGTGTCACA CCTATGTGAG GTGCACTCGC TCAGTCTCTA TTCCAGCCCC TGCATATTAT 
GCCCGGCTTG TAGCATTTAG GGCAAGGTAT CATCTGGTGG ATAAAGATCA TGACAGTGCG 
GAAGGCAGTC ATGTGTCAGG ACAGAGCAAC GGCCGGGATC CTCAGGCCTT GGCTAAGGCT 
GTGCAAATCC ACCATGATAC CCAGCACACG ATGTATTTTG CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005270635.1
CDS	277..2661
Translation	MVRHFKMQIFGDRQPGYDGKRNMYTAHPLPIGRDRVDMEVTLPGEGKDQTFKVSVQWVSVVSLQLLLEALAGHLNEVPDDSVQALDVITRHLPSMRYTPVGRSFFSPPEGYYHPLGGGREVWFGFHQSVRPAMWNMMLNIDVSATAFYRAQPIIEFMCEVLDIQNINEQTKPLTDSQRVKFTKEIRGLKVEVTHCGQMKRKYRVCNVTRRPASHQTFPLQLENGQAMECTVAQYFKQKYSLQLKYPHLPCLQVGQEQKHTYLPLEVCNIVAGQRCIKKLTDNQTSTMIKATARSAPDRQEEISRLVKSNSMVGGPDPYLKEFGIVVHNEMTELTGRVLPAPMLQYGGRNKTVATPNQGVWDMRGKQFYAGIEIKVWAVACFAPQKQCREDLLKSFTDQLRKISKDAGMPIQGQPCFCKYAQGADSVEPMFKHLKMTYVGLQLIVVILPGKTPVYAEVKRVGDTLLGMATQCVQVKNVVKTSPQTLSNLCLKINAKLGGINNVLVPHQRPSVFQQPVIFLGADVTHPPAGDGKKPSIAAVVGSMDGHPSRYCATVRVQTSRQEISQELLYSQEVIQDLTNMVRELLIQFYKSTRFKPTRIIYYRGGVSEGQMKQVAWPELIAIRKACISLEEDYRPGITYIVVQKRHHTRLFCADKTERVGKSGNVPAGTTVDSTITHPSEFDFYLCSHAGIQGTSRPSHYQVLWDDNCFTADELQLLTYQLCHTYVRCTRSVSIPAPAYYARLVAFRARYHLVDKDHDSAEGSHVSGQSNGRDPQALAKAVQIHHDTQHTMYFA

Target ORF information:

RefSeq Version	XM_005270578.3
Organism	Homo sapiens(human)
Definition	Homo sapiens argonaute RISC component 4 (AGO4), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005270578.3

ORF Insert Sequence:

ATGGTGCGGC ACTTCAAGAT GCAAATATTT GGTGATCGGC AGCCTGGGTA TGATGGCAAA 
AGAAACATGT ACACAGCACA TCCACTACCA ATTGGACGGG ATAGGGTTGA TATGGAGGTG 
ACTCTTCCAG GCGAGGGTAA AGACCAAACA TTTAAAGTGT CTGTTCAGTG GGTGTCAGTT 
GTGAGCCTTC AGTTGCTTTT AGAAGCTTTG GCTGGGCACT TGAATGAAGT CCCAGATGAC 
TCAGTACAAG CACTTGATGT TATCACAAGA CACCTTCCCT CCATGAGGTA CACCCCAGTG 
GGCCGTTCCT TTTTCTCACC CCCGGAAGGT TACTACCACC CTCTGGGAGG GGGCAGGGAG 
GTCTGGTTTG GTTTTCATCA GTCTGTGAGA CCTGCCATGT GGAATATGAT GCTCAACATT 
GATGTATCTG CAACTGCTTT CTACCGGGCT CAGCCTATCA TTGAGTTCAT GTGTGAGGTT 
TTAGACATTC AGAACATCAA TGAACAGACC AAACCTCTAA CAGACTCCCA GCGTGTCAAA 
TTTACCAAAG AAATCAGAGG TCTCAAAGTT GAGGTGACCC ACTGTGGACA GATGAAACGA 
AAATACCGAG TTTGTAATGT GACTAGACGG CCAGCCAGTC ATCAAACTTT TCCTTTGCAG 
CTAGAAAACG GTCAAGCTAT GGAATGTACA GTAGCTCAAT ATTTTAAGCA AAAGTATAGT 
CTGCAACTGA AATACCCCCA TCTTCCCTGT CTCCAAGTGG GACAAGAACA AAAGCATACA 
TACTTGCCAC TCGAGGTCTG TAATATAGTG GCAGGACAGC GATGTATCAA GAAGCTCACA 
GACAATCAGA CTTCCACAAT GATCAAAGCT ACAGCAAGAT CTGCTCCTGA CAGACAGGAA 
GAGATCAGTA GACTGGTGAA GAGCAACAGT ATGGTGGGTG GACCTGATCC ATACCTTAAA 
GAATTTGGTA TTGTTGTCCA CAATGAAATG ACAGAGCTCA CAGGCAGGGT ACTTCCAGCA 
CCAATGCTGC AATATGGAGG CCGGAATAAA ACAGTAGCCA CACCCAACCA GGGTGTCTGG 
GACATGCGAG GAAAGCAGTT TTATGCTGGC ATTGAAATTA AAGTTTGGGC AGTTGCTTGT 
TTTGCACCTC AGAAACAATG TAGGGAAGAT TTACTAAAGA GTTTCACTGA CCAGCTGCGT 
AAAATCTCTA AGGATGCAGG AATGCCCATC CAGGGTCAGC CATGTTTCTG CAAGTATGCA 
CAAGGTGCAG ACAGTGTGGA GCCTATGTTT AAACATCTGA AAATGACTTA TGTGGGCCTA 
CAGCTAATAG TGGTTATCCT GCCTGGAAAG ACACCAGTAT ATGCGGAGGT GAAACGTGTT 
GGAGATACCC TTCTAGGTAT GGCCACACAG TGTGTCCAGG TAAAAAATGT AGTGAAGACC 
TCACCTCAAA CCCTTTCCAA TCTTTGCCTG AAGATAAATG CAAAACTTGG AGGAATTAAC 
AATGTGCTTG TGCCTCATCA AAGGCCCTCG GTGTTCCAGC AGCCTGTCAT CTTCCTGGGA 
GCGGATGTCA CACACCCCCC AGCAGGGGAT GGGAAGAAAC CTTCCATTGC TGCTGTGGTT 
GGCAGTATGG ATGGCCACCC CAGCCGGTAC TGTGCCACCG TTCGGGTGCA GACTTCCCGG 
CAGGAGATCT CCCAAGAGCT CCTCTACAGT CAAGAGGTCA TCCAGGACCT GACTAACATG 
GTTCGAGAGC TGCTGATTCA GTTCTACAAA TCCACACGCT TCAAACCCAC TCGGATCATC 
TATTACCGTG GAGGGGTATC TGAGGGACAA ATGAAACAGG TAGCTTGGCC AGAACTAATA 
GCAATTCGAA AGGCATGTAT TAGCTTGGAA GAAGATTACC GGCCAGGAAT AACTTATATT 
GTGGTGCAAA AAAGACATCA CACACGACTC TTCTGTGCAG ATAAAACAGA AAGGGTAGGG 
AAAAGTGGCA ATGTACCAGC AGGCACTACA GTGGATAGTA CCATCACACA TCCATCTGAG 
TTTGACTTTT ACCTCTGTAG TCATGCAGGA ATTCAGGGAA CCAGCCGTCC CTCACATTAC 
CAGGTCTTGT GGGATGACAA CTGCTTCACT GCAGATGAAC TCCAGCTACT GACTTACCAG 
CTGTGTCACA CCTATGTGAG GTGCACTCGC TCAGTCTCTA TTCCAGCCCC TGCATATTAT 
GCCCGGCTTG TAGCATTTAG GGCAAGGTAT CATCTGGTGG ATAAAGATCA TGACAGTGCG 
GAAGGCAGTC ATGTGTCAGG ACAGAGCAAC GGCCGGGATC CTCAGGCCTT GGCTAAGGCT 
GTGCAAATCC ACCATGATAC CCAGCACACG ATGTATTTTG CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AGO4 ( XM_005270579.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_005270636.1 Homo sapiens argonaute RISC component 4 (AGO4), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OHu33098
Clone ID Related Accession (Same CDS sequence)	XM_005270578.3 , XM_005270579.3
Accession Version	XM_005270579.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2385bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	protein argonaute-4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005270579.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCGGC ACTTCAAGAT GCAAATATTT GGTGATCGGC AGCCTGGGTA TGATGGCAAA 
AGAAACATGT ACACAGCACA TCCACTACCA ATTGGACGGG ATAGGGTTGA TATGGAGGTG 
ACTCTTCCAG GCGAGGGTAA AGACCAAACA TTTAAAGTGT CTGTTCAGTG GGTGTCAGTT 
GTGAGCCTTC AGTTGCTTTT AGAAGCTTTG GCTGGGCACT TGAATGAAGT CCCAGATGAC 
TCAGTACAAG CACTTGATGT TATCACAAGA CACCTTCCCT CCATGAGGTA CACCCCAGTG 
GGCCGTTCCT TTTTCTCACC CCCGGAAGGT TACTACCACC CTCTGGGAGG GGGCAGGGAG 
GTCTGGTTTG GTTTTCATCA GTCTGTGAGA CCTGCCATGT GGAATATGAT GCTCAACATT 
GATGTATCTG CAACTGCTTT CTACCGGGCT CAGCCTATCA TTGAGTTCAT GTGTGAGGTT 
TTAGACATTC AGAACATCAA TGAACAGACC AAACCTCTAA CAGACTCCCA GCGTGTCAAA 
TTTACCAAAG AAATCAGAGG TCTCAAAGTT GAGGTGACCC ACTGTGGACA GATGAAACGA 
AAATACCGAG TTTGTAATGT GACTAGACGG CCAGCCAGTC ATCAAACTTT TCCTTTGCAG 
CTAGAAAACG GTCAAGCTAT GGAATGTACA GTAGCTCAAT ATTTTAAGCA AAAGTATAGT 
CTGCAACTGA AATACCCCCA TCTTCCCTGT CTCCAAGTGG GACAAGAACA AAAGCATACA 
TACTTGCCAC TCGAGGTCTG TAATATAGTG GCAGGACAGC GATGTATCAA GAAGCTCACA 
GACAATCAGA CTTCCACAAT GATCAAAGCT ACAGCAAGAT CTGCTCCTGA CAGACAGGAA 
GAGATCAGTA GACTGGTGAA GAGCAACAGT ATGGTGGGTG GACCTGATCC ATACCTTAAA 
GAATTTGGTA TTGTTGTCCA CAATGAAATG ACAGAGCTCA CAGGCAGGGT ACTTCCAGCA 
CCAATGCTGC AATATGGAGG CCGGAATAAA ACAGTAGCCA CACCCAACCA GGGTGTCTGG 
GACATGCGAG GAAAGCAGTT TTATGCTGGC ATTGAAATTA AAGTTTGGGC AGTTGCTTGT 
TTTGCACCTC AGAAACAATG TAGGGAAGAT TTACTAAAGA GTTTCACTGA CCAGCTGCGT 
AAAATCTCTA AGGATGCAGG AATGCCCATC CAGGGTCAGC CATGTTTCTG CAAGTATGCA 
CAAGGTGCAG ACAGTGTGGA GCCTATGTTT AAACATCTGA AAATGACTTA TGTGGGCCTA 
CAGCTAATAG TGGTTATCCT GCCTGGAAAG ACACCAGTAT ATGCGGAGGT GAAACGTGTT 
GGAGATACCC TTCTAGGTAT GGCCACACAG TGTGTCCAGG TAAAAAATGT AGTGAAGACC 
TCACCTCAAA CCCTTTCCAA TCTTTGCCTG AAGATAAATG CAAAACTTGG AGGAATTAAC 
AATGTGCTTG TGCCTCATCA AAGGCCCTCG GTGTTCCAGC AGCCTGTCAT CTTCCTGGGA 
GCGGATGTCA CACACCCCCC AGCAGGGGAT GGGAAGAAAC CTTCCATTGC TGCTGTGGTT 
GGCAGTATGG ATGGCCACCC CAGCCGGTAC TGTGCCACCG TTCGGGTGCA GACTTCCCGG 
CAGGAGATCT CCCAAGAGCT CCTCTACAGT CAAGAGGTCA TCCAGGACCT GACTAACATG 
GTTCGAGAGC TGCTGATTCA GTTCTACAAA TCCACACGCT TCAAACCCAC TCGGATCATC 
TATTACCGTG GAGGGGTATC TGAGGGACAA ATGAAACAGG TAGCTTGGCC AGAACTAATA 
GCAATTCGAA AGGCATGTAT TAGCTTGGAA GAAGATTACC GGCCAGGAAT AACTTATATT 
GTGGTGCAAA AAAGACATCA CACACGACTC TTCTGTGCAG ATAAAACAGA AAGGGTAGGG 
AAAAGTGGCA ATGTACCAGC AGGCACTACA GTGGATAGTA CCATCACACA TCCATCTGAG 
TTTGACTTTT ACCTCTGTAG TCATGCAGGA ATTCAGGGAA CCAGCCGTCC CTCACATTAC 
CAGGTCTTGT GGGATGACAA CTGCTTCACT GCAGATGAAC TCCAGCTACT GACTTACCAG 
CTGTGTCACA CCTATGTGAG GTGCACTCGC TCAGTCTCTA TTCCAGCCCC TGCATATTAT 
GCCCGGCTTG TAGCATTTAG GGCAAGGTAT CATCTGGTGG ATAAAGATCA TGACAGTGCG 
GAAGGCAGTC ATGTGTCAGG ACAGAGCAAC GGCCGGGATC CTCAGGCCTT GGCTAAGGCT 
GTGCAAATCC ACCATGATAC CCAGCACACG ATGTATTTTG CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005270636.1
CDS	85..2469
Translation	MVRHFKMQIFGDRQPGYDGKRNMYTAHPLPIGRDRVDMEVTLPGEGKDQTFKVSVQWVSVVSLQLLLEALAGHLNEVPDDSVQALDVITRHLPSMRYTPVGRSFFSPPEGYYHPLGGGREVWFGFHQSVRPAMWNMMLNIDVSATAFYRAQPIIEFMCEVLDIQNINEQTKPLTDSQRVKFTKEIRGLKVEVTHCGQMKRKYRVCNVTRRPASHQTFPLQLENGQAMECTVAQYFKQKYSLQLKYPHLPCLQVGQEQKHTYLPLEVCNIVAGQRCIKKLTDNQTSTMIKATARSAPDRQEEISRLVKSNSMVGGPDPYLKEFGIVVHNEMTELTGRVLPAPMLQYGGRNKTVATPNQGVWDMRGKQFYAGIEIKVWAVACFAPQKQCREDLLKSFTDQLRKISKDAGMPIQGQPCFCKYAQGADSVEPMFKHLKMTYVGLQLIVVILPGKTPVYAEVKRVGDTLLGMATQCVQVKNVVKTSPQTLSNLCLKINAKLGGINNVLVPHQRPSVFQQPVIFLGADVTHPPAGDGKKPSIAAVVGSMDGHPSRYCATVRVQTSRQEISQELLYSQEVIQDLTNMVRELLIQFYKSTRFKPTRIIYYRGGVSEGQMKQVAWPELIAIRKACISLEEDYRPGITYIVVQKRHHTRLFCADKTERVGKSGNVPAGTTVDSTITHPSEFDFYLCSHAGIQGTSRPSHYQVLWDDNCFTADELQLLTYQLCHTYVRCTRSVSIPAPAYYARLVAFRARYHLVDKDHDSAEGSHVSGQSNGRDPQALAKAVQIHHDTQHTMYFA

Target ORF information:

RefSeq Version	XM_005270579.3
Organism	Homo sapiens(human)
Definition	Homo sapiens argonaute RISC component 4 (AGO4), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005270579.3

ORF Insert Sequence:

ATGGTGCGGC ACTTCAAGAT GCAAATATTT GGTGATCGGC AGCCTGGGTA TGATGGCAAA 
AGAAACATGT ACACAGCACA TCCACTACCA ATTGGACGGG ATAGGGTTGA TATGGAGGTG 
ACTCTTCCAG GCGAGGGTAA AGACCAAACA TTTAAAGTGT CTGTTCAGTG GGTGTCAGTT 
GTGAGCCTTC AGTTGCTTTT AGAAGCTTTG GCTGGGCACT TGAATGAAGT CCCAGATGAC 
TCAGTACAAG CACTTGATGT TATCACAAGA CACCTTCCCT CCATGAGGTA CACCCCAGTG 
GGCCGTTCCT TTTTCTCACC CCCGGAAGGT TACTACCACC CTCTGGGAGG GGGCAGGGAG 
GTCTGGTTTG GTTTTCATCA GTCTGTGAGA CCTGCCATGT GGAATATGAT GCTCAACATT 
GATGTATCTG CAACTGCTTT CTACCGGGCT CAGCCTATCA TTGAGTTCAT GTGTGAGGTT 
TTAGACATTC AGAACATCAA TGAACAGACC AAACCTCTAA CAGACTCCCA GCGTGTCAAA 
TTTACCAAAG AAATCAGAGG TCTCAAAGTT GAGGTGACCC ACTGTGGACA GATGAAACGA 
AAATACCGAG TTTGTAATGT GACTAGACGG CCAGCCAGTC ATCAAACTTT TCCTTTGCAG 
CTAGAAAACG GTCAAGCTAT GGAATGTACA GTAGCTCAAT ATTTTAAGCA AAAGTATAGT 
CTGCAACTGA AATACCCCCA TCTTCCCTGT CTCCAAGTGG GACAAGAACA AAAGCATACA 
TACTTGCCAC TCGAGGTCTG TAATATAGTG GCAGGACAGC GATGTATCAA GAAGCTCACA 
GACAATCAGA CTTCCACAAT GATCAAAGCT ACAGCAAGAT CTGCTCCTGA CAGACAGGAA 
GAGATCAGTA GACTGGTGAA GAGCAACAGT ATGGTGGGTG GACCTGATCC ATACCTTAAA 
GAATTTGGTA TTGTTGTCCA CAATGAAATG ACAGAGCTCA CAGGCAGGGT ACTTCCAGCA 
CCAATGCTGC AATATGGAGG CCGGAATAAA ACAGTAGCCA CACCCAACCA GGGTGTCTGG 
GACATGCGAG GAAAGCAGTT TTATGCTGGC ATTGAAATTA AAGTTTGGGC AGTTGCTTGT 
TTTGCACCTC AGAAACAATG TAGGGAAGAT TTACTAAAGA GTTTCACTGA CCAGCTGCGT 
AAAATCTCTA AGGATGCAGG AATGCCCATC CAGGGTCAGC CATGTTTCTG CAAGTATGCA 
CAAGGTGCAG ACAGTGTGGA GCCTATGTTT AAACATCTGA AAATGACTTA TGTGGGCCTA 
CAGCTAATAG TGGTTATCCT GCCTGGAAAG ACACCAGTAT ATGCGGAGGT GAAACGTGTT 
GGAGATACCC TTCTAGGTAT GGCCACACAG TGTGTCCAGG TAAAAAATGT AGTGAAGACC 
TCACCTCAAA CCCTTTCCAA TCTTTGCCTG AAGATAAATG CAAAACTTGG AGGAATTAAC 
AATGTGCTTG TGCCTCATCA AAGGCCCTCG GTGTTCCAGC AGCCTGTCAT CTTCCTGGGA 
GCGGATGTCA CACACCCCCC AGCAGGGGAT GGGAAGAAAC CTTCCATTGC TGCTGTGGTT 
GGCAGTATGG ATGGCCACCC CAGCCGGTAC TGTGCCACCG TTCGGGTGCA GACTTCCCGG 
CAGGAGATCT CCCAAGAGCT CCTCTACAGT CAAGAGGTCA TCCAGGACCT GACTAACATG 
GTTCGAGAGC TGCTGATTCA GTTCTACAAA TCCACACGCT TCAAACCCAC TCGGATCATC 
TATTACCGTG GAGGGGTATC TGAGGGACAA ATGAAACAGG TAGCTTGGCC AGAACTAATA 
GCAATTCGAA AGGCATGTAT TAGCTTGGAA GAAGATTACC GGCCAGGAAT AACTTATATT 
GTGGTGCAAA AAAGACATCA CACACGACTC TTCTGTGCAG ATAAAACAGA AAGGGTAGGG 
AAAAGTGGCA ATGTACCAGC AGGCACTACA GTGGATAGTA CCATCACACA TCCATCTGAG 
TTTGACTTTT ACCTCTGTAG TCATGCAGGA ATTCAGGGAA CCAGCCGTCC CTCACATTAC 
CAGGTCTTGT GGGATGACAA CTGCTTCACT GCAGATGAAC TCCAGCTACT GACTTACCAG 
CTGTGTCACA CCTATGTGAG GTGCACTCGC TCAGTCTCTA TTCCAGCCCC TGCATATTAT 
GCCCGGCTTG TAGCATTTAG GGCAAGGTAT CATCTGGTGG ATAAAGATCA TGACAGTGCG 
GAAGGCAGTC ATGTGTCAGG ACAGAGCAAC GGCCGGGATC CTCAGGCCTT GGCTAAGGCT 
GTGCAAATCC ACCATGATAC CCAGCACACG ATGTATTTTG CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AGO4 ( XM_011540883.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011539185.1 Homo sapiens argonaute RISC component 4 (AGO4), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OHu77458
Clone ID Related Accession (Same CDS sequence)	XM_011540883.2
Accession Version	XM_011540883.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2136bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	protein argonaute-4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011540883.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTAATG TGACACATGC AAACTCTTAT TTCTTCAGGT ACACCCCAGT GGGCCGTTCC 
TTTTTCTCAC CCCCGGAAGG TTACTACCAC CCTCTGGGAG GGGGCAGGGA GGTCTGGTTT 
GGTTTTCATC AGTCTGTGAG ACCTGCCATG TGGAATATGA TGCTCAACAT TGATGTATCT 
GCAACTGCTT TCTACCGGGC TCAGCCTATC ATTGAGTTCA TGTGTGAGGT TTTAGACATT 
CAGAACATCA ATGAACAGAC CAAACCTCTA ACAGACTCCC AGCGTGTCAA ATTTACCAAA 
GAAATCAGAG GTCTCAAAGT TGAGGTGACC CACTGTGGAC AGATGAAACG AAAATACCGA 
GTTTGTAATG TGACTAGACG GCCAGCCAGT CATCAAACTT TTCCTTTGCA GCTAGAAAAC 
GGTCAAGCTA TGGAATGTAC AGTAGCTCAA TATTTTAAGC AAAAGTATAG TCTGCAACTG 
AAATACCCCC ATCTTCCCTG TCTCCAAGTG GGACAAGAAC AAAAGCATAC ATACTTGCCA 
CTCGAGGTCT GTAATATAGT GGCAGGACAG CGATGTATCA AGAAGCTCAC AGACAATCAG 
ACTTCCACAA TGATCAAAGC TACAGCAAGA TCTGCTCCTG ACAGACAGGA AGAGATCAGT 
AGACTGGTGA AGAGCAACAG TATGGTGGGT GGACCTGATC CATACCTTAA AGAATTTGGT 
ATTGTTGTCC ACAATGAAAT GACAGAGCTC ACAGGCAGGG TACTTCCAGC ACCAATGCTG 
CAATATGGAG GCCGGAATAA AACAGTAGCC ACACCCAACC AGGGTGTCTG GGACATGCGA 
GGAAAGCAGT TTTATGCTGG CATTGAAATT AAAGTTTGGG CAGTTGCTTG TTTTGCACCT 
CAGAAACAAT GTAGGGAAGA TTTACTAAAG AGTTTCACTG ACCAGCTGCG TAAAATCTCT 
AAGGATGCAG GAATGCCCAT CCAGGGTCAG CCATGTTTCT GCAAGTATGC ACAAGGTGCA 
GACAGTGTGG AGCCTATGTT TAAACATCTG AAAATGACTT ATGTGGGCCT ACAGCTAATA 
GTGGTTATCC TGCCTGGAAA GACACCAGTA TATGCGGAGG TGAAACGTGT TGGAGATACC 
CTTCTAGGTA TGGCCACACA GTGTGTCCAG GTAAAAAATG TAGTGAAGAC CTCACCTCAA 
ACCCTTTCCA ATCTTTGCCT GAAGATAAAT GCAAAACTTG GAGGAATTAA CAATGTGCTT 
GTGCCTCATC AAAGGCCCTC GGTGTTCCAG CAGCCTGTCA TCTTCCTGGG AGCGGATGTC 
ACACACCCCC CAGCAGGGGA TGGGAAGAAA CCTTCCATTG CTGCTGTGGT TGGCAGTATG 
GATGGCCACC CCAGCCGGTA CTGTGCCACC GTTCGGGTGC AGACTTCCCG GCAGGAGATC 
TCCCAAGAGC TCCTCTACAG TCAAGAGGTC ATCCAGGACC TGACTAACAT GGTTCGAGAG 
CTGCTGATTC AGTTCTACAA ATCCACACGC TTCAAACCCA CTCGGATCAT CTATTACCGT 
GGAGGGGTAT CTGAGGGACA AATGAAACAG GTAGCTTGGC CAGAACTAAT AGCAATTCGA 
AAGGCATGTA TTAGCTTGGA AGAAGATTAC CGGCCAGGAA TAACTTATAT TGTGGTGCAA 
AAAAGACATC ACACACGACT CTTCTGTGCA GATAAAACAG AAAGGGTAGG GAAAAGTGGC 
AATGTACCAG CAGGCACTAC AGTGGATAGT ACCATCACAC ATCCATCTGA GTTTGACTTT 
TACCTCTGTA GTCATGCAGG AATTCAGGGA ACCAGCCGTC CCTCACATTA CCAGGTCTTG 
TGGGATGACA ACTGCTTCAC TGCAGATGAA CTCCAGCTAC TGACTTACCA GCTGTGTCAC 
ACCTATGTGA GGTGCACTCG CTCAGTCTCT ATTCCAGCCC CTGCATATTA TGCCCGGCTT 
GTAGCATTTA GGGCAAGGTA TCATCTGGTG GATAAAGATC ATGACAGTGC GGAAGGCAGT 
CATGTGTCAG GACAGAGCAA CGGCCGGGAT CCTCAGGCCT TGGCTAAGGC TGTGCAAATC 
CACCATGATA CCCAGCACAC GATGTATTTT GCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011539185.1
CDS	130..2265
Translation	MFNVTHANSYFFRYTPVGRSFFSPPEGYYHPLGGGREVWFGFHQSVRPAMWNMMLNIDVSATAFYRAQPIIEFMCEVLDIQNINEQTKPLTDSQRVKFTKEIRGLKVEVTHCGQMKRKYRVCNVTRRPASHQTFPLQLENGQAMECTVAQYFKQKYSLQLKYPHLPCLQVGQEQKHTYLPLEVCNIVAGQRCIKKLTDNQTSTMIKATARSAPDRQEEISRLVKSNSMVGGPDPYLKEFGIVVHNEMTELTGRVLPAPMLQYGGRNKTVATPNQGVWDMRGKQFYAGIEIKVWAVACFAPQKQCREDLLKSFTDQLRKISKDAGMPIQGQPCFCKYAQGADSVEPMFKHLKMTYVGLQLIVVILPGKTPVYAEVKRVGDTLLGMATQCVQVKNVVKTSPQTLSNLCLKINAKLGGINNVLVPHQRPSVFQQPVIFLGADVTHPPAGDGKKPSIAAVVGSMDGHPSRYCATVRVQTSRQEISQELLYSQEVIQDLTNMVRELLIQFYKSTRFKPTRIIYYRGGVSEGQMKQVAWPELIAIRKACISLEEDYRPGITYIVVQKRHHTRLFCADKTERVGKSGNVPAGTTVDSTITHPSEFDFYLCSHAGIQGTSRPSHYQVLWDDNCFTADELQLLTYQLCHTYVRCTRSVSIPAPAYYARLVAFRARYHLVDKDHDSAEGSHVSGQSNGRDPQALAKAVQIHHDTQHTMYFA

Target ORF information:

RefSeq Version	XM_011540883.2
Organism	Homo sapiens(human)
Definition	Homo sapiens argonaute RISC component 4 (AGO4), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011540883.2

ORF Insert Sequence:

ATGTTTAATG TGACACATGC AAACTCTTAT TTCTTCAGGT ACACCCCAGT GGGCCGTTCC 
TTTTTCTCAC CCCCGGAAGG TTACTACCAC CCTCTGGGAG GGGGCAGGGA GGTCTGGTTT 
GGTTTTCATC AGTCTGTGAG ACCTGCCATG TGGAATATGA TGCTCAACAT TGATGTATCT 
GCAACTGCTT TCTACCGGGC TCAGCCTATC ATTGAGTTCA TGTGTGAGGT TTTAGACATT 
CAGAACATCA ATGAACAGAC CAAACCTCTA ACAGACTCCC AGCGTGTCAA ATTTACCAAA 
GAAATCAGAG GTCTCAAAGT TGAGGTGACC CACTGTGGAC AGATGAAACG AAAATACCGA 
GTTTGTAATG TGACTAGACG GCCAGCCAGT CATCAAACTT TTCCTTTGCA GCTAGAAAAC 
GGTCAAGCTA TGGAATGTAC AGTAGCTCAA TATTTTAAGC AAAAGTATAG TCTGCAACTG 
AAATACCCCC ATCTTCCCTG TCTCCAAGTG GGACAAGAAC AAAAGCATAC ATACTTGCCA 
CTCGAGGTCT GTAATATAGT GGCAGGACAG CGATGTATCA AGAAGCTCAC AGACAATCAG 
ACTTCCACAA TGATCAAAGC TACAGCAAGA TCTGCTCCTG ACAGACAGGA AGAGATCAGT 
AGACTGGTGA AGAGCAACAG TATGGTGGGT GGACCTGATC CATACCTTAA AGAATTTGGT 
ATTGTTGTCC ACAATGAAAT GACAGAGCTC ACAGGCAGGG TACTTCCAGC ACCAATGCTG 
CAATATGGAG GCCGGAATAA AACAGTAGCC ACACCCAACC AGGGTGTCTG GGACATGCGA 
GGAAAGCAGT TTTATGCTGG CATTGAAATT AAAGTTTGGG CAGTTGCTTG TTTTGCACCT 
CAGAAACAAT GTAGGGAAGA TTTACTAAAG AGTTTCACTG ACCAGCTGCG TAAAATCTCT 
AAGGATGCAG GAATGCCCAT CCAGGGTCAG CCATGTTTCT GCAAGTATGC ACAAGGTGCA 
GACAGTGTGG AGCCTATGTT TAAACATCTG AAAATGACTT ATGTGGGCCT ACAGCTAATA 
GTGGTTATCC TGCCTGGAAA GACACCAGTA TATGCGGAGG TGAAACGTGT TGGAGATACC 
CTTCTAGGTA TGGCCACACA GTGTGTCCAG GTAAAAAATG TAGTGAAGAC CTCACCTCAA 
ACCCTTTCCA ATCTTTGCCT GAAGATAAAT GCAAAACTTG GAGGAATTAA CAATGTGCTT 
GTGCCTCATC AAAGGCCCTC GGTGTTCCAG CAGCCTGTCA TCTTCCTGGG AGCGGATGTC 
ACACACCCCC CAGCAGGGGA TGGGAAGAAA CCTTCCATTG CTGCTGTGGT TGGCAGTATG 
GATGGCCACC CCAGCCGGTA CTGTGCCACC GTTCGGGTGC AGACTTCCCG GCAGGAGATC 
TCCCAAGAGC TCCTCTACAG TCAAGAGGTC ATCCAGGACC TGACTAACAT GGTTCGAGAG 
CTGCTGATTC AGTTCTACAA ATCCACACGC TTCAAACCCA CTCGGATCAT CTATTACCGT 
GGAGGGGTAT CTGAGGGACA AATGAAACAG GTAGCTTGGC CAGAACTAAT AGCAATTCGA 
AAGGCATGTA TTAGCTTGGA AGAAGATTAC CGGCCAGGAA TAACTTATAT TGTGGTGCAA 
AAAAGACATC ACACACGACT CTTCTGTGCA GATAAAACAG AAAGGGTAGG GAAAAGTGGC 
AATGTACCAG CAGGCACTAC AGTGGATAGT ACCATCACAC ATCCATCTGA GTTTGACTTT 
TACCTCTGTA GTCATGCAGG AATTCAGGGA ACCAGCCGTC CCTCACATTA CCAGGTCTTG 
TGGGATGACA ACTGCTTCAC TGCAGATGAA CTCCAGCTAC TGACTTACCA GCTGTGTCAC 
ACCTATGTGA GGTGCACTCG CTCAGTCTCT ATTCCAGCCC CTGCATATTA TGCCCGGCTT 
GTAGCATTTA GGGCAAGGTA TCATCTGGTG GATAAAGATC ATGACAGTGC GGAAGGCAGT 
CATGTGTCAG GACAGAGCAA CGGCCGGGAT CCTCAGGCCT TGGCTAAGGC TGTGCAAATC 
CACCATGATA CCCAGCACAC GATGTATTTT GCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AGO4 ( XM_024453795.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024309563.1 Homo sapiens argonaute RISC component 4 (AGO4), transcript variant X8, mRNA.

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CloneID	OHu103609
Clone ID Related Accession (Same CDS sequence)	XM_024453795.1
Accession Version	XM_024453795.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1854bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	protein argonaute-4 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTTGCC TTTTAAAAAA AATTTCAGGT CTCAAAGTTG AGGTGACCCA CTGTGGACAG 
ATGAAACGAA AATACCGAGT TTGTAATGTG ACTAGACGGC CAGCCAGTCA TCAAACTTTT 
CCTTTGCAGC TAGAAAACGG TCAAGCTATG GAATGTACAG TAGCTCAATA TTTTAAGCAA 
AAGTATAGTC TGCAACTGAA ATACCCCCAT CTTCCCTGTC TCCAAGTGGG ACAAGAACAA 
AAGCATACAT ACTTGCCACT CGAGGTCTGT AATATAGTGG CAGGACAGCG ATGTATCAAG 
AAGCTCACAG ACAATCAGAC TTCCACAATG ATCAAAGCTA CAGCAAGATC TGCTCCTGAC 
AGACAGGAAG AGATCAGTAG ACTGGTGAAG AGCAACAGTA TGGTGGGTGG ACCTGATCCA 
TACCTTAAAG AATTTGGTAT TGTTGTCCAC AATGAAATGA CAGAGCTCAC AGGCAGGGTA 
CTTCCAGCAC CAATGCTGCA ATATGGAGGC CGGAATAAAA CAGTAGCCAC ACCCAACCAG 
GGTGTCTGGG ACATGCGAGG AAAGCAGTTT TATGCTGGCA TTGAAATTAA AGTTTGGGCA 
GTTGCTTGTT TTGCACCTCA GAAACAATGT AGGGAAGATT TACTAAAGAG TTTCACTGAC 
CAGCTGCGTA AAATCTCTAA GGATGCAGGA ATGCCCATCC AGGGTCAGCC ATGTTTCTGC 
AAGTATGCAC AAGGTGCAGA CAGTGTGGAG CCTATGTTTA AACATCTGAA AATGACTTAT 
GTGGGCCTAC AGCTAATAGT GGTTATCCTG CCTGGAAAGA CACCAGTATA TGCGGAGGTG 
AAACGTGTTG GAGATACCCT TCTAGGTATG GCCACACAGT GTGTCCAGGT AAAAAATGTA 
GTGAAGACCT CACCTCAAAC CCTTTCCAAT CTTTGCCTGA AGATAAATGC AAAACTTGGA 
GGAATTAACA ATGTGCTTGT GCCTCATCAA AGGCCCTCGG TGTTCCAGCA GCCTGTCATC 
TTCCTGGGAG CGGATGTCAC ACACCCCCCA GCAGGGGATG GGAAGAAACC TTCCATTGCT 
GCTGTGGTTG GCAGTATGGA TGGCCACCCC AGCCGGTACT GTGCCACCGT TCGGGTGCAG 
ACTTCCCGGC AGGAGATCTC CCAAGAGCTC CTCTACAGTC AAGAGGTCAT CCAGGACCTG 
ACTAACATGG TTCGAGAGCT GCTGATTCAG TTCTACAAAT CCACACGCTT CAAACCCACT 
CGGATCATCT ATTACCGTGG AGGGGTATCT GAGGGACAAA TGAAACAGGT AGCTTGGCCA 
GAACTAATAG CAATTCGAAA GGCATGTATT AGCTTGGAAG AAGATTACCG GCCAGGAATA 
ACTTATATTG TGGTGCAAAA AAGACATCAC ACACGACTCT TCTGTGCAGA TAAAACAGAA 
AGGGTAGGGA AAAGTGGCAA TGTACCAGCA GGCACTACAG TGGATAGTAC CATCACACAT 
CCATCTGAGT TTGACTTTTA CCTCTGTAGT CATGCAGGAA TTCAGGGAAC CAGCCGTCCC 
TCACATTACC AGGTCTTGTG GGATGACAAC TGCTTCACTG CAGATGAACT CCAGCTACTG 
ACTTACCAGC TGTGTCACAC CTATGTGAGG TGCACTCGCT CAGTCTCTAT TCCAGCCCCT 
GCATATTATG CCCGGCTTGT AGCATTTAGG GCAAGGTATC ATCTGGTGGA TAAAGATCAT 
GACAGTGCGG AAGGCAGTCA TGTGTCAGGA CAGAGCAACG GCCGGGATCC TCAGGCCTTG 
GCTAAGGCTG TGCAAATCCA CCATGATACC CAGCACACGA TGTATTTTGC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024309563.1
CDS	1419..3272
Translation	MFCLLKKISGLKVEVTHCGQMKRKYRVCNVTRRPASHQTFPLQLENGQAMECTVAQYFKQKYSLQLKYPHLPCLQVGQEQKHTYLPLEVCNIVAGQRCIKKLTDNQTSTMIKATARSAPDRQEEISRLVKSNSMVGGPDPYLKEFGIVVHNEMTELTGRVLPAPMLQYGGRNKTVATPNQGVWDMRGKQFYAGIEIKVWAVACFAPQKQCREDLLKSFTDQLRKISKDAGMPIQGQPCFCKYAQGADSVEPMFKHLKMTYVGLQLIVVILPGKTPVYAEVKRVGDTLLGMATQCVQVKNVVKTSPQTLSNLCLKINAKLGGINNVLVPHQRPSVFQQPVIFLGADVTHPPAGDGKKPSIAAVVGSMDGHPSRYCATVRVQTSRQEISQELLYSQEVIQDLTNMVRELLIQFYKSTRFKPTRIIYYRGGVSEGQMKQVAWPELIAIRKACISLEEDYRPGITYIVVQKRHHTRLFCADKTERVGKSGNVPAGTTVDSTITHPSEFDFYLCSHAGIQGTSRPSHYQVLWDDNCFTADELQLLTYQLCHTYVRCTRSVSIPAPAYYARLVAFRARYHLVDKDHDSAEGSHVSGQSNGRDPQALAKAVQIHHDTQHTMYFA

Target ORF information:

RefSeq Version	XM_024453795.1
Organism	Homo sapiens(human)
Definition	Homo sapiens argonaute RISC component 4 (AGO4), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024453795.1

ORF Insert Sequence:

ATGTTTTGCC TTTTAAAAAA AATTTCAGGT CTCAAAGTTG AGGTGACCCA CTGTGGACAG 
ATGAAACGAA AATACCGAGT TTGTAATGTG ACTAGACGGC CAGCCAGTCA TCAAACTTTT 
CCTTTGCAGC TAGAAAACGG TCAAGCTATG GAATGTACAG TAGCTCAATA TTTTAAGCAA 
AAGTATAGTC TGCAACTGAA ATACCCCCAT CTTCCCTGTC TCCAAGTGGG ACAAGAACAA 
AAGCATACAT ACTTGCCACT CGAGGTCTGT AATATAGTGG CAGGACAGCG ATGTATCAAG 
AAGCTCACAG ACAATCAGAC TTCCACAATG ATCAAAGCTA CAGCAAGATC TGCTCCTGAC 
AGACAGGAAG AGATCAGTAG ACTGGTGAAG AGCAACAGTA TGGTGGGTGG ACCTGATCCA 
TACCTTAAAG AATTTGGTAT TGTTGTCCAC AATGAAATGA CAGAGCTCAC AGGCAGGGTA 
CTTCCAGCAC CAATGCTGCA ATATGGAGGC CGGAATAAAA CAGTAGCCAC ACCCAACCAG 
GGTGTCTGGG ACATGCGAGG AAAGCAGTTT TATGCTGGCA TTGAAATTAA AGTTTGGGCA 
GTTGCTTGTT TTGCACCTCA GAAACAATGT AGGGAAGATT TACTAAAGAG TTTCACTGAC 
CAGCTGCGTA AAATCTCTAA GGATGCAGGA ATGCCCATCC AGGGTCAGCC ATGTTTCTGC 
AAGTATGCAC AAGGTGCAGA CAGTGTGGAG CCTATGTTTA AACATCTGAA AATGACTTAT 
GTGGGCCTAC AGCTAATAGT GGTTATCCTG CCTGGAAAGA CACCAGTATA TGCGGAGGTG 
AAACGTGTTG GAGATACCCT TCTAGGTATG GCCACACAGT GTGTCCAGGT AAAAAATGTA 
GTGAAGACCT CACCTCAAAC CCTTTCCAAT CTTTGCCTGA AGATAAATGC AAAACTTGGA 
GGAATTAACA ATGTGCTTGT GCCTCATCAA AGGCCCTCGG TGTTCCAGCA GCCTGTCATC 
TTCCTGGGAG CGGATGTCAC ACACCCCCCA GCAGGGGATG GGAAGAAACC TTCCATTGCT 
GCTGTGGTTG GCAGTATGGA TGGCCACCCC AGCCGGTACT GTGCCACCGT TCGGGTGCAG 
ACTTCCCGGC AGGAGATCTC CCAAGAGCTC CTCTACAGTC AAGAGGTCAT CCAGGACCTG 
ACTAACATGG TTCGAGAGCT GCTGATTCAG TTCTACAAAT CCACACGCTT CAAACCCACT 
CGGATCATCT ATTACCGTGG AGGGGTATCT GAGGGACAAA TGAAACAGGT AGCTTGGCCA 
GAACTAATAG CAATTCGAAA GGCATGTATT AGCTTGGAAG AAGATTACCG GCCAGGAATA 
ACTTATATTG TGGTGCAAAA AAGACATCAC ACACGACTCT TCTGTGCAGA TAAAACAGAA 
AGGGTAGGGA AAAGTGGCAA TGTACCAGCA GGCACTACAG TGGATAGTAC CATCACACAT 
CCATCTGAGT TTGACTTTTA CCTCTGTAGT CATGCAGGAA TTCAGGGAAC CAGCCGTCCC 
TCACATTACC AGGTCTTGTG GGATGACAAC TGCTTCACTG CAGATGAACT CCAGCTACTG 
ACTTACCAGC TGTGTCACAC CTATGTGAGG TGCACTCGCT CAGTCTCTAT TCCAGCCCCT 
GCATATTATG CCCGGCTTGT AGCATTTAGG GCAAGGTATC ATCTGGTGGA TAAAGATCAT 
GACAGTGCGG AAGGCAGTCA TGTGTCAGGA CAGAGCAACG GCCGGGATCC TCAGGCCTTG 
GCTAAGGCTG TGCAAATCCA CCATGATACC CAGCACACGA TGTATTTTGC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Generation of catalytic human Ago4 identifies structural elements important for RNA cleavage.
RNA (New York, N.Y.)20(10)1532-8(2014 Oct)
Hauptmann J,Kater L,L?ffler P,Merkl R,Meister G

Molecular dissection of human Argonaute proteins by DNA shuffling.
Nature structural & molecular biology20(7)818-26(2013 Jul)
Sch?rmann N,Trabuco LG,Bender C,Russell RB,Grimm D

Slicing-independent RISC activation requires the argonaute PAZ domain.
Current biology : CB22(16)1536-42(2012 Aug)
Gu S,Jin L,Huang Y,Zhang F,Kay MA

Structural evolution and functional diversification analyses of argonaute protein.
Journal of cellular biochemistry113(8)2576-85(2012 Aug)
Wei KF,Wu LJ,Chen J,Chen YF,Xie DX

Argonaute identity defines the length of mature mammalian microRNAs.
Nucleic acids research40(14)6808-20(2012 Aug)
Juvvuna PK,Khandelia P,Lee LM,Makeyev EV

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AGO4 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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