EEF1A2 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	EEF1A2
Entrez Gene ID	1917
Full Name	eukaryotic translation elongation factor 1 alpha 2
Synonyms	EEF1AL,EF-1-alpha-2,EF1A,EIEE33,HS1,MRD38,STN,STNL
General protein information	Preferred Names eukaryotic translation elongation factor 1 alpha 2 Names elongation factor 1-alpha 2 eukaryotic elongation factor 1 A-2 statin-S1
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 20 Map Location: 20q13.33
Summary	This gene encodes an isoform of the alpha subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This isoform (alpha 2) is expressed in brain, heart and skeletal muscle, and the other isoform (alpha 1) is expressed in brain, placenta, lung, liver, kidney, and pancreas. This gene may be critical in the development of ovarian cancer. [provided by RefSeq, Mar 2014].
Disorder MIM:	602959
Disorder Html:	Mental retardation, autosomal dominant 38, 616393 (3); Epileptic encephalopathy, early infantile, 33, 616409 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001958.3	NP_001949.1	elongation factor 1-alpha 2
NM_001958.4	NP_001949.1	elongation factor 1-alpha 2
NM_001958.5	NP_001949.1	elongation factor 1-alpha 2

Pathways from BioSystems

Homologies

Homo sapiens (human)	EEF1A2	NP_001949.1
	LOC708096	XP_001107326.1
Drosophila melanogaster (fruit fly)	Ef1alpha100E	NP_524611.1
Canis lupus familiaris (dog)	LOC102156596	XP_005617616.1
Canis lupus familiaris (dog)	EEF1A1	NP_001184045.1
Xenopus tropicalis (tropical clawed frog)	eef1a2	NP_001011418.1
Canis lupus familiaris (dog)	LOC102155289	XP_005618552.1
Rattus norvegicus (Norway rat)	Eef1a2	NP_036792.2
Pan troglodytes (chimpanzee)	EEF1A2	XP_003954143.1
Bos taurus (cattle)	EEF1A2	NP_001032541.1
Gallus gallus (chicken)	EEF1A2	NP_001027570.1
Danio rerio (zebrafish)	eef1a2	NP_001002371.1
Mus musculus (house mouse)	Eef1a2	NP_031932.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003541	XP_003436515.1

Gene Ontology
Bibliography

Related articles in PubMed

The EEF1A2 gene expression as risk predictor in localized prostate cancer.
Worst TS, Waldbillig F, Abdelhadi A, Weis CA, Gottschalt M, Steidler A, von Hardenberg J, Michel MS, Erben P
BMC urology17(1)86(2017 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

We report a pair of siblings carrying a homozygous missense mutation p.P333L in EEF1A2 who exhibited global developmental delay, failure to thrive, dilated cardiomyopathy and epilepsy, ultimately leading to death in early childhood. EEF1A2 appears to be critical for normal heart function in humans, and its deficiency results in clinical abnormalities in neurologic function as well as in skeletal and cardiac muscle defects
Title: Homozygous EEF1A2 mutation causes dilated cardiomyopathy, failure to thrive, global developmental delay, epilepsy and early death.

Differential gene expression analysis demonstrated significant upregulation of PDZK1IP1, EEF1A2 and RPL41 (ENSG00000279483) genes in the intrahepatic cholangiocarcinoma samples when compared with the matched paratumor samples.
Title: Overexpression of PDZK1IP1, EEF1A2 and RPL41 genes in intrahepatic cholangiocarcinoma.

In both cases, a de novo recurrent heterozygous mutation in EEF1A2 [c.364G>A (p.E122K)] was identified by whole-exome sequencing. CONCLUSION: This report provides clinical data on epileptic encephalopathy in patients with EEF1A2 mutation. Continuous high-voltage delta activity seen over both parietal areas may be a unique manifestation of EEF1A2 mutation.
Title: Two cases of early-onset myoclonic seizures with continuous parietal delta activity caused by EEF1A2 mutations.

eEF1A2 is highly expressed in hepatocellular carcinoma. Its silencing significantly decreases HCC tumorigenesis, likely by inhibiting PI3K/Akt/NF-kappaB signaling.
Title: Eukaryotic elongation factor-1? 2 knockdown inhibits hepatocarcinogenesis by suppressing PI3K/Akt/NF-?B signaling.

Our results provide novel information on the intracellular distribution and interaction of eEF1A isoforms.
Title: New insights on the interaction between the isoforms 1 and 2 of human translation elongation factor 1A.

The following EEF1A2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EEF1A2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu20020	NM_001958.5 Latest version!	Homo sapiens eukaryotic translation elongation factor 1 alpha 2 (EEF1A2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
View Old Accession Versions >>
OHu20020	NM_001958.3	Homo sapiens eukaryotic translation elongation factor 1 alpha 2 (EEF1A2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
OHu20020	NM_001958.4	Homo sapiens eukaryotic translation elongation factor 1 alpha 2 (EEF1A2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

EEF1A2 ( NM_001958.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001949.1 Homo sapiens eukaryotic translation elongation factor 1 alpha 2 (EEF1A2), mRNA.

Price & Availability↑

CloneID	OHu20020
Clone ID Related Accession (Same CDS sequence)	NM_001958.3 , NM_001958.4 , NM_001958.5
Accession Version	NM_001958.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1392bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-02
Organism	Homo sapiens(human)
Product	elongation factor 1-alpha 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BM476361.1, BC110409.1 and BQ944047.1. This sequence is a reference standard in the RefSeqGene project. On Apr 25, 2012 this sequence version replaced NM_001958.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC110409.1, ERR279848.8836.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: full length.

ATGGGCAAGG AGAAGACCCA CATCAACATC GTGGTCATCG GCCACGTGGA CTCCGGAAAG 
TCCACCACCA CGGGCCACCT CATCTACAAA TGCGGAGGTA TTGACAAAAG GACCATTGAG 
AAGTTCGAGA AGGAGGCGGC TGAGATGGGG AAGGGATCCT TCAAGTATGC CTGGGTGCTG 
GACAAGCTGA AGGCGGAGCG TGAGCGCGGC ATCACCATCG ACATCTCCCT CTGGAAGTTC 
GAGACCACCA AGTACTACAT CACCATCATC GATGCCCCCG GCCACCGCGA CTTCATCAAG 
AACATGATCA CGGGTACATC CCAGGCGGAC TGCGCAGTGC TGATCGTGGC GGCGGGCGTG 
GGCGAGTTCG AGGCGGGCAT CTCCAAGAAT GGGCAGACGC GGGAGCATGC CCTGCTGGCC 
TACACGCTGG GTGTGAAGCA GCTCATCGTG GGCGTGAACA AAATGGACTC CACAGAGCCG 
GCCTACAGCG AGAAGCGCTA CGACGAGATC GTCAAGGAAG TCAGCGCCTA CATCAAGAAG 
ATCGGCTACA ACCCGGCCAC CGTGCCCTTT GTGCCCATCT CCGGCTGGCA CGGTGACAAC 
ATGCTGGAGC CCTCCCCCAA CATGCCGTGG TTCAAGGGCT GGAAGGTGGA GCGTAAGGAG 
GGCAACGCAA GCGGCGTGTC CCTGCTGGAG GCCCTGGACA CCATCCTGCC CCCCACGCGC 
CCCACGGACA AGCCCCTGCG CCTGCCGCTG CAGGACGTGT ACAAGATTGG CGGCATTGGC 
ACGGTGCCCG TGGGCCGGGT GGAGACCGGC ATCCTGCGGC CGGGCATGGT GGTGACCTTT 
GCGCCAGTGA ACATCACCAC TGAGGTGAAG TCAGTGGAGA TGCACCACGA GGCTCTGAGC 
GAAGCTCTGC CCGGCGACAA CGTCGGCTTC AATGTGAAGA ACGTGTCGGT GAAGGACATC 
CGGCGGGGCA ACGTGTGTGG GGACAGCAAG TCTGACCCGC CGCAGGAGGC TGCTCAGTTC 
ACCTCCCAGG TCATCATCCT GAACCACCCG GGGCAGATTA GCGCCGGCTA CTCCCCGGTC 
ATCGACTGCC ACACAGCCCA CATCGCCTGC AAGTTTGCGG AGCTGAAGGA GAAGATTGAC 
CGGCGCTCTG GCAAGAAGCT GGAGGACAAC CCCAAGTCCC TGAAGTCTGG AGACGCGGCC 
ATCGTGGAGA TGGTGCCGGG AAAGCCCATG TGTGTGGAGA GCTTCTCCCA GTACCCGCCT 
CTCGGCCGCT TCGCCGTGCG CGACATGAGG CAGACGGTGG CCGTAGGCGT CATCAAGAAC 
GTGGAGAAGA AGAGCGGCGG CGCCGGCAAG GTCACCAAGT CGGCGCAGAA GGCGCAGAAG 
GCGGGCAAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001949.1
CDS	330..1721
Translation	MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAAEMGKGSFKYAWVLDKLKAERERGITIDISLWKFETTKYYITIIDAPGHRDFIKNMITGTSQADCAVLIVAAGVGEFEAGISKNGQTREHALLAYTLGVKQLIVGVNKMDSTEPAYSEKRYDEIVKEVSAYIKKIGYNPATVPFVPISGWHGDNMLEPSPNMPWFKGWKVERKEGNASGVSLLEALDTILPPTRPTDKPLRLPLQDVYKIGGIGTVPVGRVETGILRPGMVVTFAPVNITTEVKSVEMHHEALSEALPGDNVGFNVKNVSVKDIRRGNVCGDSKSDPPQEAAQFTSQVIILNHPGQISAGYSPVIDCHTAHIACKFAELKEKIDRRSGKKLEDNPKSLKSGDAAIVEMVPGKPMCVESFSQYPPLGRFAVRDMRQTVAVGVIKNVEKKSGGAGKVTKSAQKAQKAGK

Target ORF information:

RefSeq Version	NM_001958.3
Organism	Homo sapiens(human)
Definition	Homo sapiens eukaryotic translation elongation factor 1 alpha 2 (EEF1A2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001958.3

ORF Insert Sequence:

ATGGGCAAGG AGAAGACCCA CATCAACATC GTGGTCATCG GCCACGTGGA CTCCGGAAAG 
TCCACCACCA CGGGCCACCT CATCTACAAA TGCGGAGGTA TTGACAAAAG GACCATTGAG 
AAGTTCGAGA AGGAGGCGGC TGAGATGGGG AAGGGATCCT TCAAGTATGC CTGGGTGCTG 
GACAAGCTGA AGGCGGAGCG TGAGCGCGGC ATCACCATCG ACATCTCCCT CTGGAAGTTC 
GAGACCACCA AGTACTACAT CACCATCATC GATGCCCCCG GCCACCGCGA CTTCATCAAG 
AACATGATCA CGGGTACATC CCAGGCGGAC TGCGCAGTGC TGATCGTGGC GGCGGGCGTG 
GGCGAGTTCG AGGCGGGCAT CTCCAAGAAT GGGCAGACGC GGGAGCATGC CCTGCTGGCC 
TACACGCTGG GTGTGAAGCA GCTCATCGTG GGCGTGAACA AAATGGACTC CACAGAGCCG 
GCCTACAGCG AGAAGCGCTA CGACGAGATC GTCAAGGAAG TCAGCGCCTA CATCAAGAAG 
ATCGGCTACA ACCCGGCCAC CGTGCCCTTT GTGCCCATCT CCGGCTGGCA CGGTGACAAC 
ATGCTGGAGC CCTCCCCCAA CATGCCGTGG TTCAAGGGCT GGAAGGTGGA GCGTAAGGAG 
GGCAACGCAA GCGGCGTGTC CCTGCTGGAG GCCCTGGACA CCATCCTGCC CCCCACGCGC 
CCCACGGACA AGCCCCTGCG CCTGCCGCTG CAGGACGTGT ACAAGATTGG CGGCATTGGC 
ACGGTGCCCG TGGGCCGGGT GGAGACCGGC ATCCTGCGGC CGGGCATGGT GGTGACCTTT 
GCGCCAGTGA ACATCACCAC TGAGGTGAAG TCAGTGGAGA TGCACCACGA GGCTCTGAGC 
GAAGCTCTGC CCGGCGACAA CGTCGGCTTC AATGTGAAGA ACGTGTCGGT GAAGGACATC 
CGGCGGGGCA ACGTGTGTGG GGACAGCAAG TCTGACCCGC CGCAGGAGGC TGCTCAGTTC 
ACCTCCCAGG TCATCATCCT GAACCACCCG GGGCAGATTA GCGCCGGCTA CTCCCCGGTC 
ATCGACTGCC ACACAGCCCA CATCGCCTGC AAGTTTGCGG AGCTGAAGGA GAAGATTGAC 
CGGCGCTCTG GCAAGAAGCT GGAGGACAAC CCCAAGTCCC TGAAGTCTGG AGACGCGGCC 
ATCGTGGAGA TGGTGCCGGG AAAGCCCATG TGTGTGGAGA GCTTCTCCCA GTACCCGCCT 
CTCGGCCGCT TCGCCGTGCG CGACATGAGG CAGACGGTGG CCGTAGGCGT CATCAAGAAC 
GTGGAGAAGA AGAGCGGCGG CGCCGGCAAG GTCACCAAGT CGGCGCAGAA GGCGCAGAAG 
GCGGGCAAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EEF1A2 ( NM_001958.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_001949.1 Homo sapiens eukaryotic translation elongation factor 1 alpha 2 (EEF1A2), mRNA.

Price & Availability↑

CloneID	OHu20020
Clone ID Related Accession (Same CDS sequence)	NM_001958.3 , NM_001958.4 , NM_001958.5
Accession Version	NM_001958.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1392bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-05
Organism	Homo sapiens(human)
Product	elongation factor 1-alpha 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC110409.1. On Nov 22, 2018 this sequence version replaced NM_001958.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC110409.1, X70940.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGGCAAGG AGAAGACCCA CATCAACATC GTGGTCATCG GCCACGTGGA CTCCGGAAAG 
TCCACCACCA CGGGCCACCT CATCTACAAA TGCGGAGGTA TTGACAAAAG GACCATTGAG 
AAGTTCGAGA AGGAGGCGGC TGAGATGGGG AAGGGATCCT TCAAGTATGC CTGGGTGCTG 
GACAAGCTGA AGGCGGAGCG TGAGCGCGGC ATCACCATCG ACATCTCCCT CTGGAAGTTC 
GAGACCACCA AGTACTACAT CACCATCATC GATGCCCCCG GCCACCGCGA CTTCATCAAG 
AACATGATCA CGGGTACATC CCAGGCGGAC TGCGCAGTGC TGATCGTGGC GGCGGGCGTG 
GGCGAGTTCG AGGCGGGCAT CTCCAAGAAT GGGCAGACGC GGGAGCATGC CCTGCTGGCC 
TACACGCTGG GTGTGAAGCA GCTCATCGTG GGCGTGAACA AAATGGACTC CACAGAGCCG 
GCCTACAGCG AGAAGCGCTA CGACGAGATC GTCAAGGAAG TCAGCGCCTA CATCAAGAAG 
ATCGGCTACA ACCCGGCCAC CGTGCCCTTT GTGCCCATCT CCGGCTGGCA CGGTGACAAC 
ATGCTGGAGC CCTCCCCCAA CATGCCGTGG TTCAAGGGCT GGAAGGTGGA GCGTAAGGAG 
GGCAACGCAA GCGGCGTGTC CCTGCTGGAG GCCCTGGACA CCATCCTGCC CCCCACGCGC 
CCCACGGACA AGCCCCTGCG CCTGCCGCTG CAGGACGTGT ACAAGATTGG CGGCATTGGC 
ACGGTGCCCG TGGGCCGGGT GGAGACCGGC ATCCTGCGGC CGGGCATGGT GGTGACCTTT 
GCGCCAGTGA ACATCACCAC TGAGGTGAAG TCAGTGGAGA TGCACCACGA GGCTCTGAGC 
GAAGCTCTGC CCGGCGACAA CGTCGGCTTC AATGTGAAGA ACGTGTCGGT GAAGGACATC 
CGGCGGGGCA ACGTGTGTGG GGACAGCAAG TCTGACCCGC CGCAGGAGGC TGCTCAGTTC 
ACCTCCCAGG TCATCATCCT GAACCACCCG GGGCAGATTA GCGCCGGCTA CTCCCCGGTC 
ATCGACTGCC ACACAGCCCA CATCGCCTGC AAGTTTGCGG AGCTGAAGGA GAAGATTGAC 
CGGCGCTCTG GCAAGAAGCT GGAGGACAAC CCCAAGTCCC TGAAGTCTGG AGACGCGGCC 
ATCGTGGAGA TGGTGCCGGG AAAGCCCATG TGTGTGGAGA GCTTCTCCCA GTACCCGCCT 
CTCGGCCGCT TCGCCGTGCG CGACATGAGG CAGACGGTGG CCGTAGGCGT CATCAAGAAC 
GTGGAGAAGA AGAGCGGCGG CGCCGGCAAG GTCACCAAGT CGGCGCAGAA GGCGCAGAAG 
GCGGGCAAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001949.1
CDS	98..1489
Translation	MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAAEMGKGSFKYAWVLDKLKAERERGITIDISLWKFETTKYYITIIDAPGHRDFIKNMITGTSQADCAVLIVAAGVGEFEAGISKNGQTREHALLAYTLGVKQLIVGVNKMDSTEPAYSEKRYDEIVKEVSAYIKKIGYNPATVPFVPISGWHGDNMLEPSPNMPWFKGWKVERKEGNASGVSLLEALDTILPPTRPTDKPLRLPLQDVYKIGGIGTVPVGRVETGILRPGMVVTFAPVNITTEVKSVEMHHEALSEALPGDNVGFNVKNVSVKDIRRGNVCGDSKSDPPQEAAQFTSQVIILNHPGQISAGYSPVIDCHTAHIACKFAELKEKIDRRSGKKLEDNPKSLKSGDAAIVEMVPGKPMCVESFSQYPPLGRFAVRDMRQTVAVGVIKNVEKKSGGAGKVTKSAQKAQKAGK

Target ORF information:

RefSeq Version	NM_001958.4
Organism	Homo sapiens(human)
Definition	Homo sapiens eukaryotic translation elongation factor 1 alpha 2 (EEF1A2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001958.4

ORF Insert Sequence:

ATGGGCAAGG AGAAGACCCA CATCAACATC GTGGTCATCG GCCACGTGGA CTCCGGAAAG 
TCCACCACCA CGGGCCACCT CATCTACAAA TGCGGAGGTA TTGACAAAAG GACCATTGAG 
AAGTTCGAGA AGGAGGCGGC TGAGATGGGG AAGGGATCCT TCAAGTATGC CTGGGTGCTG 
GACAAGCTGA AGGCGGAGCG TGAGCGCGGC ATCACCATCG ACATCTCCCT CTGGAAGTTC 
GAGACCACCA AGTACTACAT CACCATCATC GATGCCCCCG GCCACCGCGA CTTCATCAAG 
AACATGATCA CGGGTACATC CCAGGCGGAC TGCGCAGTGC TGATCGTGGC GGCGGGCGTG 
GGCGAGTTCG AGGCGGGCAT CTCCAAGAAT GGGCAGACGC GGGAGCATGC CCTGCTGGCC 
TACACGCTGG GTGTGAAGCA GCTCATCGTG GGCGTGAACA AAATGGACTC CACAGAGCCG 
GCCTACAGCG AGAAGCGCTA CGACGAGATC GTCAAGGAAG TCAGCGCCTA CATCAAGAAG 
ATCGGCTACA ACCCGGCCAC CGTGCCCTTT GTGCCCATCT CCGGCTGGCA CGGTGACAAC 
ATGCTGGAGC CCTCCCCCAA CATGCCGTGG TTCAAGGGCT GGAAGGTGGA GCGTAAGGAG 
GGCAACGCAA GCGGCGTGTC CCTGCTGGAG GCCCTGGACA CCATCCTGCC CCCCACGCGC 
CCCACGGACA AGCCCCTGCG CCTGCCGCTG CAGGACGTGT ACAAGATTGG CGGCATTGGC 
ACGGTGCCCG TGGGCCGGGT GGAGACCGGC ATCCTGCGGC CGGGCATGGT GGTGACCTTT 
GCGCCAGTGA ACATCACCAC TGAGGTGAAG TCAGTGGAGA TGCACCACGA GGCTCTGAGC 
GAAGCTCTGC CCGGCGACAA CGTCGGCTTC AATGTGAAGA ACGTGTCGGT GAAGGACATC 
CGGCGGGGCA ACGTGTGTGG GGACAGCAAG TCTGACCCGC CGCAGGAGGC TGCTCAGTTC 
ACCTCCCAGG TCATCATCCT GAACCACCCG GGGCAGATTA GCGCCGGCTA CTCCCCGGTC 
ATCGACTGCC ACACAGCCCA CATCGCCTGC AAGTTTGCGG AGCTGAAGGA GAAGATTGAC 
CGGCGCTCTG GCAAGAAGCT GGAGGACAAC CCCAAGTCCC TGAAGTCTGG AGACGCGGCC 
ATCGTGGAGA TGGTGCCGGG AAAGCCCATG TGTGTGGAGA GCTTCTCCCA GTACCCGCCT 
CTCGGCCGCT TCGCCGTGCG CGACATGAGG CAGACGGTGG CCGTAGGCGT CATCAAGAAC 
GTGGAGAAGA AGAGCGGCGG CGCCGGCAAG GTCACCAAGT CGGCGCAGAA GGCGCAGAAG 
GCGGGCAAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EEF1A2 ( NM_001958.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_001949.1 Homo sapiens eukaryotic translation elongation factor 1 alpha 2 (EEF1A2), mRNA.

Price & Availability↑

CloneID	OHu20020
Clone ID Related Accession (Same CDS sequence)	NM_001958.3 , NM_001958.4 , NM_001958.5
Accession Version	NM_001958.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1392bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-09
Organism	Homo sapiens(human)
Product	elongation factor 1-alpha 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL121829.30. On Aug 31, 2019 this sequence version replaced NM_001958.4. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC110409.1, X70940.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000217182.6/ ENSP00000217182.3 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGGCAAGG AGAAGACCCA CATCAACATC GTGGTCATCG GCCACGTGGA CTCCGGAAAG 
TCCACCACCA CGGGCCACCT CATCTACAAA TGCGGAGGTA TTGACAAAAG GACCATTGAG 
AAGTTCGAGA AGGAGGCGGC TGAGATGGGG AAGGGATCCT TCAAGTATGC CTGGGTGCTG 
GACAAGCTGA AGGCGGAGCG TGAGCGCGGC ATCACCATCG ACATCTCCCT CTGGAAGTTC 
GAGACCACCA AGTACTACAT CACCATCATC GATGCCCCCG GCCACCGCGA CTTCATCAAG 
AACATGATCA CGGGTACATC CCAGGCGGAC TGCGCAGTGC TGATCGTGGC GGCGGGCGTG 
GGCGAGTTCG AGGCGGGCAT CTCCAAGAAT GGGCAGACGC GGGAGCATGC CCTGCTGGCC 
TACACGCTGG GTGTGAAGCA GCTCATCGTG GGCGTGAACA AAATGGACTC CACAGAGCCG 
GCCTACAGCG AGAAGCGCTA CGACGAGATC GTCAAGGAAG TCAGCGCCTA CATCAAGAAG 
ATCGGCTACA ACCCGGCCAC CGTGCCCTTT GTGCCCATCT CCGGCTGGCA CGGTGACAAC 
ATGCTGGAGC CCTCCCCCAA CATGCCGTGG TTCAAGGGCT GGAAGGTGGA GCGTAAGGAG 
GGCAACGCAA GCGGCGTGTC CCTGCTGGAG GCCCTGGACA CCATCCTGCC CCCCACGCGC 
CCCACGGACA AGCCCCTGCG CCTGCCGCTG CAGGACGTGT ACAAGATTGG CGGCATTGGC 
ACGGTGCCCG TGGGCCGGGT GGAGACCGGC ATCCTGCGGC CGGGCATGGT GGTGACCTTT 
GCGCCAGTGA ACATCACCAC TGAGGTGAAG TCAGTGGAGA TGCACCACGA GGCTCTGAGC 
GAAGCTCTGC CCGGCGACAA CGTCGGCTTC AATGTGAAGA ACGTGTCGGT GAAGGACATC 
CGGCGGGGCA ACGTGTGTGG GGACAGCAAG TCTGACCCGC CGCAGGAGGC TGCTCAGTTC 
ACCTCCCAGG TCATCATCCT GAACCACCCG GGGCAGATTA GCGCCGGCTA CTCCCCGGTC 
ATCGACTGCC ACACAGCCCA CATCGCCTGC AAGTTTGCGG AGCTGAAGGA GAAGATTGAC 
CGGCGCTCTG GCAAGAAGCT GGAGGACAAC CCCAAGTCCC TGAAGTCTGG AGACGCGGCC 
ATCGTGGAGA TGGTGCCGGG AAAGCCCATG TGTGTGGAGA GCTTCTCCCA GTACCCGCCT 
CTCGGCCGCT TCGCCGTGCG CGACATGAGG CAGACGGTGG CCGTAGGCGT CATCAAGAAC 
GTGGAGAAGA AGAGCGGCGG CGCCGGCAAG GTCACCAAGT CGGCGCAGAA GGCGCAGAAG 
GCGGGCAAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001949.1
CDS	98..1489
Translation	MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAAEMGKGSFKYAWVLDKLKAERERGITIDISLWKFETTKYYITIIDAPGHRDFIKNMITGTSQADCAVLIVAAGVGEFEAGISKNGQTREHALLAYTLGVKQLIVGVNKMDSTEPAYSEKRYDEIVKEVSAYIKKIGYNPATVPFVPISGWHGDNMLEPSPNMPWFKGWKVERKEGNASGVSLLEALDTILPPTRPTDKPLRLPLQDVYKIGGIGTVPVGRVETGILRPGMVVTFAPVNITTEVKSVEMHHEALSEALPGDNVGFNVKNVSVKDIRRGNVCGDSKSDPPQEAAQFTSQVIILNHPGQISAGYSPVIDCHTAHIACKFAELKEKIDRRSGKKLEDNPKSLKSGDAAIVEMVPGKPMCVESFSQYPPLGRFAVRDMRQTVAVGVIKNVEKKSGGAGKVTKSAQKAQKAGK

Target ORF information:

RefSeq Version	NM_001958.5
Organism	Homo sapiens(human)
Definition	Homo sapiens eukaryotic translation elongation factor 1 alpha 2 (EEF1A2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001958.5

ORF Insert Sequence:

ATGGGCAAGG AGAAGACCCA CATCAACATC GTGGTCATCG GCCACGTGGA CTCCGGAAAG 
TCCACCACCA CGGGCCACCT CATCTACAAA TGCGGAGGTA TTGACAAAAG GACCATTGAG 
AAGTTCGAGA AGGAGGCGGC TGAGATGGGG AAGGGATCCT TCAAGTATGC CTGGGTGCTG 
GACAAGCTGA AGGCGGAGCG TGAGCGCGGC ATCACCATCG ACATCTCCCT CTGGAAGTTC 
GAGACCACCA AGTACTACAT CACCATCATC GATGCCCCCG GCCACCGCGA CTTCATCAAG 
AACATGATCA CGGGTACATC CCAGGCGGAC TGCGCAGTGC TGATCGTGGC GGCGGGCGTG 
GGCGAGTTCG AGGCGGGCAT CTCCAAGAAT GGGCAGACGC GGGAGCATGC CCTGCTGGCC 
TACACGCTGG GTGTGAAGCA GCTCATCGTG GGCGTGAACA AAATGGACTC CACAGAGCCG 
GCCTACAGCG AGAAGCGCTA CGACGAGATC GTCAAGGAAG TCAGCGCCTA CATCAAGAAG 
ATCGGCTACA ACCCGGCCAC CGTGCCCTTT GTGCCCATCT CCGGCTGGCA CGGTGACAAC 
ATGCTGGAGC CCTCCCCCAA CATGCCGTGG TTCAAGGGCT GGAAGGTGGA GCGTAAGGAG 
GGCAACGCAA GCGGCGTGTC CCTGCTGGAG GCCCTGGACA CCATCCTGCC CCCCACGCGC 
CCCACGGACA AGCCCCTGCG CCTGCCGCTG CAGGACGTGT ACAAGATTGG CGGCATTGGC 
ACGGTGCCCG TGGGCCGGGT GGAGACCGGC ATCCTGCGGC CGGGCATGGT GGTGACCTTT 
GCGCCAGTGA ACATCACCAC TGAGGTGAAG TCAGTGGAGA TGCACCACGA GGCTCTGAGC 
GAAGCTCTGC CCGGCGACAA CGTCGGCTTC AATGTGAAGA ACGTGTCGGT GAAGGACATC 
CGGCGGGGCA ACGTGTGTGG GGACAGCAAG TCTGACCCGC CGCAGGAGGC TGCTCAGTTC 
ACCTCCCAGG TCATCATCCT GAACCACCCG GGGCAGATTA GCGCCGGCTA CTCCCCGGTC 
ATCGACTGCC ACACAGCCCA CATCGCCTGC AAGTTTGCGG AGCTGAAGGA GAAGATTGAC 
CGGCGCTCTG GCAAGAAGCT GGAGGACAAC CCCAAGTCCC TGAAGTCTGG AGACGCGGCC 
ATCGTGGAGA TGGTGCCGGG AAAGCCCATG TGTGTGGAGA GCTTCTCCCA GTACCCGCCT 
CTCGGCCGCT TCGCCGTGCG CGACATGAGG CAGACGGTGG CCGTAGGCGT CATCAAGAAC 
GTGGAGAAGA AGAGCGGCGG CGCCGGCAAG GTCACCAAGT CGGCGCAGAA GGCGCAGAAG 
GCGGGCAAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The EEF1A2 gene expression as risk predictor in localized prostate cancer.
BMC urology17(1)86(2017 Sep)
Worst TS,Waldbillig F,Abdelhadi A,Weis CA,Gottschalt M,Steidler A,von Hardenberg J,Michel MS,Erben P

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EEF1A2 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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