ECE1 gene cDNA ORF clone, Homo sapiens(human)

mRNA	Protein	Name
XM_017000511.1	XP_016856000.1	endothelin-converting enzyme 1 isoform X2
NM_001113348.1	NP_001106819.1	endothelin-converting enzyme 1 isoform 4
XM_011540872.2	XP_011539174.1	endothelin-converting enzyme 1 isoform X1
NM_001397.3	NP_001388.1	endothelin-converting enzyme 1 isoform 1
NM_001113347.1	NP_001106818.1	endothelin-converting enzyme 1 isoform 3
XM_011540873.2	XP_011539175.1	endothelin-converting enzyme 1 isoform X2
XM_006710398.2	XP_006710461.1	endothelin-converting enzyme 1 isoform X2
NM_001113348.2	NP_001106819.1	endothelin-converting enzyme 1 isoform 4
NM_001397.2	NP_001388.1	endothelin-converting enzyme 1 isoform 1
NM_001113349.1	NP_001106820.1	endothelin-converting enzyme 1 isoform 2
NM_001113349.2	NP_001106820.1	endothelin-converting enzyme 1 isoform 2
NM_001113347.2	NP_001106818.1	endothelin-converting enzyme 1 isoform 3

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu16445	NM_001397.3 Latest version!	Homo sapiens endothelin converting enzyme 1 (ECE1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu19118	NM_001113348.2 Latest version!	Homo sapiens endothelin converting enzyme 1 (ECE1), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$379.50-$531.30 $759.00
OHu33089	XM_011540873.2 Latest version!	Homo sapiens endothelin converting enzyme 1 (ECE1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$391.30 $559.00
OHu33089	XM_017000511.1 Latest version!	Homo sapiens endothelin converting enzyme 1 (ECE1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$391.30 $559.00
OHu33089	XM_006710398.2 Latest version!	Homo sapiens endothelin converting enzyme 1 (ECE1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$391.30 $559.00
OHu16364	NM_001113347.2 Latest version!	Homo sapiens endothelin converting enzyme 1 (ECE1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$538.30 $769.00
OHu16471	NM_001113349.2 Latest version!	Homo sapiens endothelin converting enzyme 1 (ECE1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$538.30 $769.00
OHu57423	XM_011540872.2 Latest version!	Homo sapiens endothelin converting enzyme 1 (ECE1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
View Old Accession Versions >>
OHu19118	NM_001113348.1	Homo sapiens endothelin converting enzyme 1 (ECE1), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$379.50-$531.30 $759.00
OHu16445	NM_001397.2	Homo sapiens endothelin converting enzyme 1 (ECE1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu16471	NM_001113349.1	Homo sapiens endothelin converting enzyme 1 (ECE1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$538.30 $769.00
OHu16364	NM_001113347.1	Homo sapiens endothelin converting enzyme 1 (ECE1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$538.30 $769.00
Hide Old Accession Versions >>

ECE1 ( NM_001397.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001388.1 Homo sapiens endothelin converting enzyme 1 (ECE1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu16445
Clone ID Related Accession (Same CDS sequence)	NM_001397.3 , NM_001397.2
Accession Version	NM_001397.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2313bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-01
Organism	Homo sapiens(human)
Product	endothelin-converting enzyme 1 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC117256.1, Z35307.1, AL031005.2, BC106022.1 and BU733112.1. On Nov 22, 2018 this sequence version replaced NM_001397.2. Transcript Variant: This variant (1) encodes the longest protein (isoform 1 also known as isoform b). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.258431.1, SRR1660809.141391.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000374893.11/ ENSP00000364028.6 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGCGGGGCG TGTGGCCGCC CCCGGTGTCC GCCCTGCTGT CGGCGCTGGG GATGTCGACG 
TACAAGCGGG CCACGCTGGA CGAGGAGGAC CTGGTGGACT CGCTCTCCGA GGGCGACGCA 
TACCCCAACG GCCTGCAGGT GAACTTCCAC AGCCCCCGGA GTGGCCAGAG GTGCTGGGCT 
GCACGGACCC AGGTGGAGAA GCGGCTGGTG GTGTTGGTGG TACTTCTGGC GGCAGGACTG 
GTGGCCTGCT TGGCAGCACT GGGCATCCAG TACCAGACAA GATCCCCCTC TGTGTGCCTG 
AGCGAAGCTT GTGTCTCAGT GACCAGCTCC ATCTTGAGCT CCATGGACCC CACAGTGGAC 
CCCTGCCATG ACTTCTTCAG CTACGCCTGT GGGGGCTGGA TCAAGGCCAA CCCAGTCCCT 
GATGGCCACT CACGCTGGGG GACCTTCAGC AACCTCTGGG AACACAACCA AGCAATCATC 
AAGCACCTCC TCGAAAACTC CACGGCCAGC GTGAGCGAGG CAGAGAGAAA GGCGCAAGTA 
TACTACCGTG CGTGCATGAA CGAGACCAGG ATCGAGGAGC TCAGGGCCAA ACCTCTAATG 
GAGTTGATTG AGAGGCTCGG GGGCTGGAAC ATCACAGGTC CCTGGGCCAA GGACAACTTC 
CAGGACACCC TGCAGGTGGT CACCGCCCAC TACCGCACCT CACCCTTCTT CTCTGTCTAT 
GTCAGTGCCG ATTCCAAGAA CTCCAACAGC AACGTGATCC AGGTGGACCA GTCTGGCCTG 
GGCTTGCCCT CGAGAGACTA TTACCTGAAC AAAACTGAAA ACGAGAAGGT GCTGACCGGA 
TATCTGAACT ACATGGTCCA GCTGGGGAAG CTGCTGGGCG GCGGGGACGA GGAGGCCATC 
CGGCCCCAGA TGCAGCAGAT CTTGGACTTT GAGACGGCAC TGGCCAACAT CACCATCCCA 
CAGGAGAAGC GCCGTGATGA GGAGCTCATC TACCACAAAG TGACGGCAGC CGAGCTGCAG 
ACCTTGGCAC CCGCCATCAA CTGGTTGCCT TTTCTCAACA CCATCTTCTA CCCCGTGGAG 
ATCAATGAAT CCGAGCCTAT TGTGGTCTAT GACAAGGAAT ACCTTGAGCA GATCTCCACT 
CTCATCAACA CCACCGACAG ATGCCTGCTC AACAACTACA TGATCTGGAA CCTGGTGCGG 
AAAACAAGCT CCTTCCTTGA CCAGCGCTTT CAGGACGCCG ATGAGAAGTT CATGGAAGTC 
ATGTACGGGA CCAAGAAGAC CTGTCTTCCT CGCTGGAAGT TTTGCGTGAG TGACACAGAA 
AACAACCTGG GCTTTGCGTT GGGCCCCATG TTTGTCAAAG CAACCTTCGC CGAGGACAGC 
AAGAGCATAG CCACCGAGAT CATCCTGGAG ATTAAGAAGG CATTTGAGGA AAGCCTGAGC 
ACCCTGAAGT GGATGGATGA GGAAACCCGA AAATCAGCCA AGGAAAAGGC CGATGCCATC 
TACAACATGA TAGGATACCC CAACTTCATC ATGGATCCCA AGGAGCTGGA CAAAGTGTTT 
AATGACTACA CTGCAGTTCC AGACCTCTAC TTTGAAAATG CCATGCGGTT TTTCAACTTC 
TCATGGAGGG TCACTGCCGA TCAGCTCAGG AAAGCCCCCA ACAGAGATCA GTGGAGCATG 
ACCCCGCCCA TGGTGAACGC CTACTACTCG CCCACCAAGA ATGAGATTGT GTTTCCGGCC 
GGGATCCTGC AGGCACCATT CTACACACGC TCCTCACCCA AGGCCTTAAA CTTTGGTGGC 
ATAGGTGTCG TCGTGGGCCA TGAGCTGACT CATGCTTTTG ATGATCAAGG ACGGGAGTAT 
GACAAGGACG GGAACCTCCG GCCATGGTGG AAGAACTCAT CCGTGGAGGC CTTCAAGCGT 
CAGACCGAGT GCATGGTAGA GCAGTACAGC AACTACAGCG TGAACGGGGA GCCGGTGAAC 
GGGCGGCACA CCCTGGGGGA GAACATCGCC GACAACGGGG GTCTCAAGGC GGCCTATCGG 
GCTTACCAGA ACTGGGTGAA GAAGAACGGG GCTGAGCACT CGCTCCCCAC CCTGGGCCTC 
ACCAATAACC AGCTCTTCTT CCTGGGCTTT GCACAGGTCT GGTGCTCCGT CCGCACACCT 
GAGAGCTCCC ACGAAGGCCT CATCACCGAT CCCCACAGCC CCTCTCGCTT CCGGGTCATC 
GGCTCCCTCT CCAATTCCAA GGAGTTCTCA GAACACTTCC GCTGCCCACC TGGCTCACCC 
ATGAACCCGC CTCACAAGTG CGAAGTCTGG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001388.1
CDS	50..2362
Translation	MRGVWPPPVSALLSALGMSTYKRATLDEEDLVDSLSEGDAYPNGLQVNFHSPRSGQRCWAARTQVEKRLVVLVVLLAAGLVACLAALGIQYQTRSPSVCLSEACVSVTSSILSSMDPTVDPCHDFFSYACGGWIKANPVPDGHSRWGTFSNLWEHNQAIIKHLLENSTASVSEAERKAQVYYRACMNETRIEELRAKPLMELIERLGGWNITGPWAKDNFQDTLQVVTAHYRTSPFFSVYVSADSKNSNSNVIQVDQSGLGLPSRDYYLNKTENEKVLTGYLNYMVQLGKLLGGGDEEAIRPQMQQILDFETALANITIPQEKRRDEELIYHKVTAAELQTLAPAINWLPFLNTIFYPVEINESEPIVVYDKEYLEQISTLINTTDRCLLNNYMIWNLVRKTSSFLDQRFQDADEKFMEVMYGTKKTCLPRWKFCVSDTENNLGFALGPMFVKATFAEDSKSIATEIILEIKKAFEESLSTLKWMDEETRKSAKEKADAIYNMIGYPNFIMDPKELDKVFNDYTAVPDLYFENAMRFFNFSWRVTADQLRKAPNRDQWSMTPPMVNAYYSPTKNEIVFPAGILQAPFYTRSSPKALNFGGIGVVVGHELTHAFDDQGREYDKDGNLRPWWKNSSVEAFKRQTECMVEQYSNYSVNGEPVNGRHTLGENIADNGGLKAAYRAYQNWVKKNGAEHSLPTLGLTNNQLFFLGFAQVWCSVRTPESSHEGLITDPHSPSRFRVIGSLSNSKEFSEHFRCPPGSPMNPPHKCEVW

Target ORF information:

RefSeq Version	NM_001397.3
Organism	Homo sapiens(human)
Definition	Homo sapiens endothelin converting enzyme 1 (ECE1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001397.3

ORF Insert Sequence:

ATGCGGGGCG TGTGGCCGCC CCCGGTGTCC GCCCTGCTGT CGGCGCTGGG GATGTCGACG 
TACAAGCGGG CCACGCTGGA CGAGGAGGAC CTGGTGGACT CGCTCTCCGA GGGCGACGCA 
TACCCCAACG GCCTGCAGGT GAACTTCCAC AGCCCCCGGA GTGGCCAGAG GTGCTGGGCT 
GCACGGACCC AGGTGGAGAA GCGGCTGGTG GTGTTGGTGG TACTTCTGGC GGCAGGACTG 
GTGGCCTGCT TGGCAGCACT GGGCATCCAG TACCAGACAA GATCCCCCTC TGTGTGCCTG 
AGCGAAGCTT GTGTCTCAGT GACCAGCTCC ATCTTGAGCT CCATGGACCC CACAGTGGAC 
CCCTGCCATG ACTTCTTCAG CTACGCCTGT GGGGGCTGGA TCAAGGCCAA CCCAGTCCCT 
GATGGCCACT CACGCTGGGG GACCTTCAGC AACCTCTGGG AACACAACCA AGCAATCATC 
AAGCACCTCC TCGAAAACTC CACGGCCAGC GTGAGCGAGG CAGAGAGAAA GGCGCAAGTA 
TACTACCGTG CGTGCATGAA CGAGACCAGG ATCGAGGAGC TCAGGGCCAA ACCTCTAATG 
GAGTTGATTG AGAGGCTCGG GGGCTGGAAC ATCACAGGTC CCTGGGCCAA GGACAACTTC 
CAGGACACCC TGCAGGTGGT CACCGCCCAC TACCGCACCT CACCCTTCTT CTCTGTCTAT 
GTCAGTGCCG ATTCCAAGAA CTCCAACAGC AACGTGATCC AGGTGGACCA GTCTGGCCTG 
GGCTTGCCCT CGAGAGACTA TTACCTGAAC AAAACTGAAA ACGAGAAGGT GCTGACCGGA 
TATCTGAACT ACATGGTCCA GCTGGGGAAG CTGCTGGGCG GCGGGGACGA GGAGGCCATC 
CGGCCCCAGA TGCAGCAGAT CTTGGACTTT GAGACGGCAC TGGCCAACAT CACCATCCCA 
CAGGAGAAGC GCCGTGATGA GGAGCTCATC TACCACAAAG TGACGGCAGC CGAGCTGCAG 
ACCTTGGCAC CCGCCATCAA CTGGTTGCCT TTTCTCAACA CCATCTTCTA CCCCGTGGAG 
ATCAATGAAT CCGAGCCTAT TGTGGTCTAT GACAAGGAAT ACCTTGAGCA GATCTCCACT 
CTCATCAACA CCACCGACAG ATGCCTGCTC AACAACTACA TGATCTGGAA CCTGGTGCGG 
AAAACAAGCT CCTTCCTTGA CCAGCGCTTT CAGGACGCCG ATGAGAAGTT CATGGAAGTC 
ATGTACGGGA CCAAGAAGAC CTGTCTTCCT CGCTGGAAGT TTTGCGTGAG TGACACAGAA 
AACAACCTGG GCTTTGCGTT GGGCCCCATG TTTGTCAAAG CAACCTTCGC CGAGGACAGC 
AAGAGCATAG CCACCGAGAT CATCCTGGAG ATTAAGAAGG CATTTGAGGA AAGCCTGAGC 
ACCCTGAAGT GGATGGATGA GGAAACCCGA AAATCAGCCA AGGAAAAGGC CGATGCCATC 
TACAACATGA TAGGATACCC CAACTTCATC ATGGATCCCA AGGAGCTGGA CAAAGTGTTT 
AATGACTACA CTGCAGTTCC AGACCTCTAC TTTGAAAATG CCATGCGGTT TTTCAACTTC 
TCATGGAGGG TCACTGCCGA TCAGCTCAGG AAAGCCCCCA ACAGAGATCA GTGGAGCATG 
ACCCCGCCCA TGGTGAACGC CTACTACTCG CCCACCAAGA ATGAGATTGT GTTTCCGGCC 
GGGATCCTGC AGGCACCATT CTACACACGC TCCTCACCCA AGGCCTTAAA CTTTGGTGGC 
ATAGGTGTCG TCGTGGGCCA TGAGCTGACT CATGCTTTTG ATGATCAAGG ACGGGAGTAT 
GACAAGGACG GGAACCTCCG GCCATGGTGG AAGAACTCAT CCGTGGAGGC CTTCAAGCGT 
CAGACCGAGT GCATGGTAGA GCAGTACAGC AACTACAGCG TGAACGGGGA GCCGGTGAAC 
GGGCGGCACA CCCTGGGGGA GAACATCGCC GACAACGGGG GTCTCAAGGC GGCCTATCGG 
GCTTACCAGA ACTGGGTGAA GAAGAACGGG GCTGAGCACT CGCTCCCCAC CCTGGGCCTC 
ACCAATAACC AGCTCTTCTT CCTGGGCTTT GCACAGGTCT GGTGCTCCGT CCGCACACCT 
GAGAGCTCCC ACGAAGGCCT CATCACCGAT CCCCACAGCC CCTCTCGCTT CCGGGTCATC 
GGCTCCCTCT CCAATTCCAA GGAGTTCTCA GAACACTTCC GCTGCCCACC TGGCTCACCC 
ATGAACCCGC CTCACAAGTG CGAAGTCTGG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ECE1 ( NM_001397.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001388.1 Homo sapiens endothelin converting enzyme 1 (ECE1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu16445
Clone ID Related Accession (Same CDS sequence)	NM_001397.3 , NM_001397.2
Accession Version	NM_001397.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2313bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-29
Organism	Homo sapiens(human)
Product	endothelin-converting enzyme 1 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC117256.1, Z35307.1, AL031005.2, BC106022.1 and BU733112.1. On Jan 9, 2008 this sequence version replaced NM_001397.1. Transcript Variant: This variant (1) encodes the longest protein (isoform 1 also known as isoform b). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC117256.1, SRR1660809.258431.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCGGGGCG TGTGGCCGCC CCCGGTGTCC GCCCTGCTGT CGGCGCTGGG GATGTCGACG 
TACAAGCGGG CCACGCTGGA CGAGGAGGAC CTGGTGGACT CGCTCTCCGA GGGCGACGCA 
TACCCCAACG GCCTGCAGGT GAACTTCCAC AGCCCCCGGA GTGGCCAGAG GTGCTGGGCT 
GCACGGACCC AGGTGGAGAA GCGGCTGGTG GTGTTGGTGG TACTTCTGGC GGCAGGACTG 
GTGGCCTGCT TGGCAGCACT GGGCATCCAG TACCAGACAA GATCCCCCTC TGTGTGCCTG 
AGCGAAGCTT GTGTCTCAGT GACCAGCTCC ATCTTGAGCT CCATGGACCC CACAGTGGAC 
CCCTGCCATG ACTTCTTCAG CTACGCCTGT GGGGGCTGGA TCAAGGCCAA CCCAGTCCCT 
GATGGCCACT CACGCTGGGG GACCTTCAGC AACCTCTGGG AACACAACCA AGCAATCATC 
AAGCACCTCC TCGAAAACTC CACGGCCAGC GTGAGCGAGG CAGAGAGAAA GGCGCAAGTA 
TACTACCGTG CGTGCATGAA CGAGACCAGG ATCGAGGAGC TCAGGGCCAA ACCTCTAATG 
GAGTTGATTG AGAGGCTCGG GGGCTGGAAC ATCACAGGTC CCTGGGCCAA GGACAACTTC 
CAGGACACCC TGCAGGTGGT CACCGCCCAC TACCGCACCT CACCCTTCTT CTCTGTCTAT 
GTCAGTGCCG ATTCCAAGAA CTCCAACAGC AACGTGATCC AGGTGGACCA GTCTGGCCTG 
GGCTTGCCCT CGAGAGACTA TTACCTGAAC AAAACTGAAA ACGAGAAGGT GCTGACCGGA 
TATCTGAACT ACATGGTCCA GCTGGGGAAG CTGCTGGGCG GCGGGGACGA GGAGGCCATC 
CGGCCCCAGA TGCAGCAGAT CTTGGACTTT GAGACGGCAC TGGCCAACAT CACCATCCCA 
CAGGAGAAGC GCCGTGATGA GGAGCTCATC TACCACAAAG TGACGGCAGC CGAGCTGCAG 
ACCTTGGCAC CCGCCATCAA CTGGTTGCCT TTTCTCAACA CCATCTTCTA CCCCGTGGAG 
ATCAATGAAT CCGAGCCTAT TGTGGTCTAT GACAAGGAAT ACCTTGAGCA GATCTCCACT 
CTCATCAACA CCACCGACAG ATGCCTGCTC AACAACTACA TGATCTGGAA CCTGGTGCGG 
AAAACAAGCT CCTTCCTTGA CCAGCGCTTT CAGGACGCCG ATGAGAAGTT CATGGAAGTC 
ATGTACGGGA CCAAGAAGAC CTGTCTTCCT CGCTGGAAGT TTTGCGTGAG TGACACAGAA 
AACAACCTGG GCTTTGCGTT GGGCCCCATG TTTGTCAAAG CAACCTTCGC CGAGGACAGC 
AAGAGCATAG CCACCGAGAT CATCCTGGAG ATTAAGAAGG CATTTGAGGA AAGCCTGAGC 
ACCCTGAAGT GGATGGATGA GGAAACCCGA AAATCAGCCA AGGAAAAGGC CGATGCCATC 
TACAACATGA TAGGATACCC CAACTTCATC ATGGATCCCA AGGAGCTGGA CAAAGTGTTT 
AATGACTACA CTGCAGTTCC AGACCTCTAC TTTGAAAATG CCATGCGGTT TTTCAACTTC 
TCATGGAGGG TCACTGCCGA TCAGCTCAGG AAAGCCCCCA ACAGAGATCA GTGGAGCATG 
ACCCCGCCCA TGGTGAACGC CTACTACTCG CCCACCAAGA ATGAGATTGT GTTTCCGGCC 
GGGATCCTGC AGGCACCATT CTACACACGC TCCTCACCCA AGGCCTTAAA CTTTGGTGGC 
ATAGGTGTCG TCGTGGGCCA TGAGCTGACT CATGCTTTTG ATGATCAAGG ACGGGAGTAT 
GACAAGGACG GGAACCTCCG GCCATGGTGG AAGAACTCAT CCGTGGAGGC CTTCAAGCGT 
CAGACCGAGT GCATGGTAGA GCAGTACAGC AACTACAGCG TGAACGGGGA GCCGGTGAAC 
GGGCGGCACA CCCTGGGGGA GAACATCGCC GACAACGGGG GTCTCAAGGC GGCCTATCGG 
GCTTACCAGA ACTGGGTGAA GAAGAACGGG GCTGAGCACT CGCTCCCCAC CCTGGGCCTC 
ACCAATAACC AGCTCTTCTT CCTGGGCTTT GCACAGGTCT GGTGCTCCGT CCGCACACCT 
GAGAGCTCCC ACGAAGGCCT CATCACCGAT CCCCACAGCC CCTCTCGCTT CCGGGTCATC 
GGCTCCCTCT CCAATTCCAA GGAGTTCTCA GAACACTTCC GCTGCCCACC TGGCTCACCC 
ATGAACCCGC CTCACAAGTG CGAAGTCTGG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001388.1
CDS	76..2388
Translation	MRGVWPPPVSALLSALGMSTYKRATLDEEDLVDSLSEGDAYPNGLQVNFHSPRSGQRCWAARTQVEKRLVVLVVLLAAGLVACLAALGIQYQTRSPSVCLSEACVSVTSSILSSMDPTVDPCHDFFSYACGGWIKANPVPDGHSRWGTFSNLWEHNQAIIKHLLENSTASVSEAERKAQVYYRACMNETRIEELRAKPLMELIERLGGWNITGPWAKDNFQDTLQVVTAHYRTSPFFSVYVSADSKNSNSNVIQVDQSGLGLPSRDYYLNKTENEKVLTGYLNYMVQLGKLLGGGDEEAIRPQMQQILDFETALANITIPQEKRRDEELIYHKVTAAELQTLAPAINWLPFLNTIFYPVEINESEPIVVYDKEYLEQISTLINTTDRCLLNNYMIWNLVRKTSSFLDQRFQDADEKFMEVMYGTKKTCLPRWKFCVSDTENNLGFALGPMFVKATFAEDSKSIATEIILEIKKAFEESLSTLKWMDEETRKSAKEKADAIYNMIGYPNFIMDPKELDKVFNDYTAVPDLYFENAMRFFNFSWRVTADQLRKAPNRDQWSMTPPMVNAYYSPTKNEIVFPAGILQAPFYTRSSPKALNFGGIGVVVGHELTHAFDDQGREYDKDGNLRPWWKNSSVEAFKRQTECMVEQYSNYSVNGEPVNGRHTLGENIADNGGLKAAYRAYQNWVKKNGAEHSLPTLGLTNNQLFFLGFAQVWCSVRTPESSHEGLITDPHSPSRFRVIGSLSNSKEFSEHFRCPPGSPMNPPHKCEVW

Target ORF information:

RefSeq Version	NM_001397.2
Organism	Homo sapiens(human)
Definition	Homo sapiens endothelin converting enzyme 1 (ECE1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001397.2

ORF Insert Sequence:

ATGCGGGGCG TGTGGCCGCC CCCGGTGTCC GCCCTGCTGT CGGCGCTGGG GATGTCGACG 
TACAAGCGGG CCACGCTGGA CGAGGAGGAC CTGGTGGACT CGCTCTCCGA GGGCGACGCA 
TACCCCAACG GCCTGCAGGT GAACTTCCAC AGCCCCCGGA GTGGCCAGAG GTGCTGGGCT 
GCACGGACCC AGGTGGAGAA GCGGCTGGTG GTGTTGGTGG TACTTCTGGC GGCAGGACTG 
GTGGCCTGCT TGGCAGCACT GGGCATCCAG TACCAGACAA GATCCCCCTC TGTGTGCCTG 
AGCGAAGCTT GTGTCTCAGT GACCAGCTCC ATCTTGAGCT CCATGGACCC CACAGTGGAC 
CCCTGCCATG ACTTCTTCAG CTACGCCTGT GGGGGCTGGA TCAAGGCCAA CCCAGTCCCT 
GATGGCCACT CACGCTGGGG GACCTTCAGC AACCTCTGGG AACACAACCA AGCAATCATC 
AAGCACCTCC TCGAAAACTC CACGGCCAGC GTGAGCGAGG CAGAGAGAAA GGCGCAAGTA 
TACTACCGTG CGTGCATGAA CGAGACCAGG ATCGAGGAGC TCAGGGCCAA ACCTCTAATG 
GAGTTGATTG AGAGGCTCGG GGGCTGGAAC ATCACAGGTC CCTGGGCCAA GGACAACTTC 
CAGGACACCC TGCAGGTGGT CACCGCCCAC TACCGCACCT CACCCTTCTT CTCTGTCTAT 
GTCAGTGCCG ATTCCAAGAA CTCCAACAGC AACGTGATCC AGGTGGACCA GTCTGGCCTG 
GGCTTGCCCT CGAGAGACTA TTACCTGAAC AAAACTGAAA ACGAGAAGGT GCTGACCGGA 
TATCTGAACT ACATGGTCCA GCTGGGGAAG CTGCTGGGCG GCGGGGACGA GGAGGCCATC 
CGGCCCCAGA TGCAGCAGAT CTTGGACTTT GAGACGGCAC TGGCCAACAT CACCATCCCA 
CAGGAGAAGC GCCGTGATGA GGAGCTCATC TACCACAAAG TGACGGCAGC CGAGCTGCAG 
ACCTTGGCAC CCGCCATCAA CTGGTTGCCT TTTCTCAACA CCATCTTCTA CCCCGTGGAG 
ATCAATGAAT CCGAGCCTAT TGTGGTCTAT GACAAGGAAT ACCTTGAGCA GATCTCCACT 
CTCATCAACA CCACCGACAG ATGCCTGCTC AACAACTACA TGATCTGGAA CCTGGTGCGG 
AAAACAAGCT CCTTCCTTGA CCAGCGCTTT CAGGACGCCG ATGAGAAGTT CATGGAAGTC 
ATGTACGGGA CCAAGAAGAC CTGTCTTCCT CGCTGGAAGT TTTGCGTGAG TGACACAGAA 
AACAACCTGG GCTTTGCGTT GGGCCCCATG TTTGTCAAAG CAACCTTCGC CGAGGACAGC 
AAGAGCATAG CCACCGAGAT CATCCTGGAG ATTAAGAAGG CATTTGAGGA AAGCCTGAGC 
ACCCTGAAGT GGATGGATGA GGAAACCCGA AAATCAGCCA AGGAAAAGGC CGATGCCATC 
TACAACATGA TAGGATACCC CAACTTCATC ATGGATCCCA AGGAGCTGGA CAAAGTGTTT 
AATGACTACA CTGCAGTTCC AGACCTCTAC TTTGAAAATG CCATGCGGTT TTTCAACTTC 
TCATGGAGGG TCACTGCCGA TCAGCTCAGG AAAGCCCCCA ACAGAGATCA GTGGAGCATG 
ACCCCGCCCA TGGTGAACGC CTACTACTCG CCCACCAAGA ATGAGATTGT GTTTCCGGCC 
GGGATCCTGC AGGCACCATT CTACACACGC TCCTCACCCA AGGCCTTAAA CTTTGGTGGC 
ATAGGTGTCG TCGTGGGCCA TGAGCTGACT CATGCTTTTG ATGATCAAGG ACGGGAGTAT 
GACAAGGACG GGAACCTCCG GCCATGGTGG AAGAACTCAT CCGTGGAGGC CTTCAAGCGT 
CAGACCGAGT GCATGGTAGA GCAGTACAGC AACTACAGCG TGAACGGGGA GCCGGTGAAC 
GGGCGGCACA CCCTGGGGGA GAACATCGCC GACAACGGGG GTCTCAAGGC GGCCTATCGG 
GCTTACCAGA ACTGGGTGAA GAAGAACGGG GCTGAGCACT CGCTCCCCAC CCTGGGCCTC 
ACCAATAACC AGCTCTTCTT CCTGGGCTTT GCACAGGTCT GGTGCTCCGT CCGCACACCT 
GAGAGCTCCC ACGAAGGCCT CATCACCGAT CCCCACAGCC CCTCTCGCTT CCGGGTCATC 
GGCTCCCTCT CCAATTCCAA GGAGTTCTCA GAACACTTCC GCTGCCCACC TGGCTCACCC 
ATGAACCCGC CTCACAAGTG CGAAGTCTGG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ECE1 ( NM_001113348.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001106819.1 Homo sapiens endothelin converting enzyme 1 (ECE1), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OHu19118
Clone ID Related Accession (Same CDS sequence)	NM_001113348.1 , NM_001113348.2
Accession Version	NM_001113348.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2265bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-03
Organism	Homo sapiens(human)
Product	endothelin-converting enzyme 1 isoform 4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB290492.1, AL031728.12, AB031742.1, AL031005.2, BC106022.1 and BU733112.1. This sequence is a reference standard in the RefSeqGene project. Transcript Variant: This variant (4) differs in the 5' UTR and coding region, compared to variant 1, resulting in a shorter protein (isoform 4 also known as isoform c) that has a distinct N-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.216561.1, SRR1660803.121079.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGATGTCGA CGTACAAGCG GGCCACGCTG GACGAGGAGG ACCTGGTGGA CTCGCTCTCC 
GAGGGCGACG CATACCCCAA CGGCCTGCAG GTGAACTTCC ACAGCCCCCG GAGTGGCCAG 
AGGTGCTGGG CTGCACGGAC CCAGGTGGAG AAGCGGCTGG TGGTGTTGGT GGTACTTCTG 
GCGGCAGGAC TGGTGGCCTG CTTGGCAGCA CTGGGCATCC AGTACCAGAC AAGATCCCCC 
TCTGTGTGCC TGAGCGAAGC TTGTGTCTCA GTGACCAGCT CCATCTTGAG CTCCATGGAC 
CCCACAGTGG ACCCCTGCCA TGACTTCTTC AGCTACGCCT GTGGGGGCTG GATCAAGGCC 
AACCCAGTCC CTGATGGCCA CTCACGCTGG GGGACCTTCA GCAACCTCTG GGAACACAAC 
CAAGCAATCA TCAAGCACCT CCTCGAAAAC TCCACGGCCA GCGTGAGCGA GGCAGAGAGA 
AAGGCGCAAG TATACTACCG TGCGTGCATG AACGAGACCA GGATCGAGGA GCTCAGGGCC 
AAACCTCTAA TGGAGTTGAT TGAGAGGCTC GGGGGCTGGA ACATCACAGG TCCCTGGGCC 
AAGGACAACT TCCAGGACAC CCTGCAGGTG GTCACCGCCC ACTACCGCAC CTCACCCTTC 
TTCTCTGTCT ATGTCAGTGC CGATTCCAAG AACTCCAACA GCAACGTGAT CCAGGTGGAC 
CAGTCTGGCC TGGGCTTGCC CTCGAGAGAC TATTACCTGA ACAAAACTGA AAACGAGAAG 
GTGCTGACCG GATATCTGAA CTACATGGTC CAGCTGGGGA AGCTGCTGGG CGGCGGGGAC 
GAGGAGGCCA TCCGGCCCCA GATGCAGCAG ATCTTGGACT TTGAGACGGC ACTGGCCAAC 
ATCACCATCC CACAGGAGAA GCGCCGTGAT GAGGAGCTCA TCTACCACAA AGTGACGGCA 
GCCGAGCTGC AGACCTTGGC ACCCGCCATC AACTGGTTGC CTTTTCTCAA CACCATCTTC 
TACCCCGTGG AGATCAATGA ATCCGAGCCT ATTGTGGTCT ATGACAAGGA ATACCTTGAG 
CAGATCTCCA CTCTCATCAA CACCACCGAC AGATGCCTGC TCAACAACTA CATGATCTGG 
AACCTGGTGC GGAAAACAAG CTCCTTCCTT GACCAGCGCT TTCAGGACGC CGATGAGAAG 
TTCATGGAAG TCATGTACGG GACCAAGAAG ACCTGTCTTC CTCGCTGGAA GTTTTGCGTG 
AGTGACACAG AAAACAACCT GGGCTTTGCG TTGGGCCCCA TGTTTGTCAA AGCAACCTTC 
GCCGAGGACA GCAAGAGCAT AGCCACCGAG ATCATCCTGG AGATTAAGAA GGCATTTGAG 
GAAAGCCTGA GCACCCTGAA GTGGATGGAT GAGGAAACCC GAAAATCAGC CAAGGAAAAG 
GCCGATGCCA TCTACAACAT GATAGGATAC CCCAACTTCA TCATGGATCC CAAGGAGCTG 
GACAAAGTGT TTAATGACTA CACTGCAGTT CCAGACCTCT ACTTTGAAAA TGCCATGCGG 
TTTTTCAACT TCTCATGGAG GGTCACTGCC GATCAGCTCA GGAAAGCCCC CAACAGAGAT 
CAGTGGAGCA TGACCCCGCC CATGGTGAAC GCCTACTACT CGCCCACCAA GAATGAGATT 
GTGTTTCCGG CCGGGATCCT GCAGGCACCA TTCTACACAC GCTCCTCACC CAAGGCCTTA 
AACTTTGGTG GCATAGGTGT CGTCGTGGGC CATGAGCTGA CTCATGCTTT TGATGATCAA 
GGACGGGAGT ATGACAAGGA CGGGAACCTC CGGCCATGGT GGAAGAACTC ATCCGTGGAG 
GCCTTCAAGC GTCAGACCGA GTGCATGGTA GAGCAGTACA GCAACTACAG CGTGAACGGG 
GAGCCGGTGA ACGGGCGGCA CACCCTGGGG GAGAACATCG CCGACAACGG GGGTCTCAAG 
GCGGCCTATC GGGCTTACCA GAACTGGGTG AAGAAGAACG GGGCTGAGCA CTCGCTCCCC 
ACCCTGGGCC TCACCAATAA CCAGCTCTTC TTCCTGGGCT TTGCACAGGT CTGGTGCTCC 
GTCCGCACAC CTGAGAGCTC CCACGAAGGC CTCATCACCG ATCCCCACAG CCCCTCTCGC 
TTCCGGGTCA TCGGCTCCCT CTCCAATTCC AAGGAGTTCT CAGAACACTT CCGCTGCCCA 
CCTGGCTCAC CCATGAACCC GCCTCACAAG TGCGAAGTCT GGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001106819.1
CDS	164..2428
Translation	MMSTYKRATLDEEDLVDSLSEGDAYPNGLQVNFHSPRSGQRCWAARTQVEKRLVVLVVLLAAGLVACLAALGIQYQTRSPSVCLSEACVSVTSSILSSMDPTVDPCHDFFSYACGGWIKANPVPDGHSRWGTFSNLWEHNQAIIKHLLENSTASVSEAERKAQVYYRACMNETRIEELRAKPLMELIERLGGWNITGPWAKDNFQDTLQVVTAHYRTSPFFSVYVSADSKNSNSNVIQVDQSGLGLPSRDYYLNKTENEKVLTGYLNYMVQLGKLLGGGDEEAIRPQMQQILDFETALANITIPQEKRRDEELIYHKVTAAELQTLAPAINWLPFLNTIFYPVEINESEPIVVYDKEYLEQISTLINTTDRCLLNNYMIWNLVRKTSSFLDQRFQDADEKFMEVMYGTKKTCLPRWKFCVSDTENNLGFALGPMFVKATFAEDSKSIATEIILEIKKAFEESLSTLKWMDEETRKSAKEKADAIYNMIGYPNFIMDPKELDKVFNDYTAVPDLYFENAMRFFNFSWRVTADQLRKAPNRDQWSMTPPMVNAYYSPTKNEIVFPAGILQAPFYTRSSPKALNFGGIGVVVGHELTHAFDDQGREYDKDGNLRPWWKNSSVEAFKRQTECMVEQYSNYSVNGEPVNGRHTLGENIADNGGLKAAYRAYQNWVKKNGAEHSLPTLGLTNNQLFFLGFAQVWCSVRTPESSHEGLITDPHSPSRFRVIGSLSNSKEFSEHFRCPPGSPMNPPHKCEVW

Target ORF information:

RefSeq Version	NM_001113348.1
Organism	Homo sapiens(human)
Definition	Homo sapiens endothelin converting enzyme 1 (ECE1), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001113348.1

ORF Insert Sequence:

ATGATGTCGA CGTACAAGCG GGCCACGCTG GACGAGGAGG ACCTGGTGGA CTCGCTCTCC 
GAGGGCGACG CATACCCCAA CGGCCTGCAG GTGAACTTCC ACAGCCCCCG GAGTGGCCAG 
AGGTGCTGGG CTGCACGGAC CCAGGTGGAG AAGCGGCTGG TGGTGTTGGT GGTACTTCTG 
GCGGCAGGAC TGGTGGCCTG CTTGGCAGCA CTGGGCATCC AGTACCAGAC AAGATCCCCC 
TCTGTGTGCC TGAGCGAAGC TTGTGTCTCA GTGACCAGCT CCATCTTGAG CTCCATGGAC 
CCCACAGTGG ACCCCTGCCA TGACTTCTTC AGCTACGCCT GTGGGGGCTG GATCAAGGCC 
AACCCAGTCC CTGATGGCCA CTCACGCTGG GGGACCTTCA GCAACCTCTG GGAACACAAC 
CAAGCAATCA TCAAGCACCT CCTCGAAAAC TCCACGGCCA GCGTGAGCGA GGCAGAGAGA 
AAGGCGCAAG TATACTACCG TGCGTGCATG AACGAGACCA GGATCGAGGA GCTCAGGGCC 
AAACCTCTAA TGGAGTTGAT TGAGAGGCTC GGGGGCTGGA ACATCACAGG TCCCTGGGCC 
AAGGACAACT TCCAGGACAC CCTGCAGGTG GTCACCGCCC ACTACCGCAC CTCACCCTTC 
TTCTCTGTCT ATGTCAGTGC CGATTCCAAG AACTCCAACA GCAACGTGAT CCAGGTGGAC 
CAGTCTGGCC TGGGCTTGCC CTCGAGAGAC TATTACCTGA ACAAAACTGA AAACGAGAAG 
GTGCTGACCG GATATCTGAA CTACATGGTC CAGCTGGGGA AGCTGCTGGG CGGCGGGGAC 
GAGGAGGCCA TCCGGCCCCA GATGCAGCAG ATCTTGGACT TTGAGACGGC ACTGGCCAAC 
ATCACCATCC CACAGGAGAA GCGCCGTGAT GAGGAGCTCA TCTACCACAA AGTGACGGCA 
GCCGAGCTGC AGACCTTGGC ACCCGCCATC AACTGGTTGC CTTTTCTCAA CACCATCTTC 
TACCCCGTGG AGATCAATGA ATCCGAGCCT ATTGTGGTCT ATGACAAGGA ATACCTTGAG 
CAGATCTCCA CTCTCATCAA CACCACCGAC AGATGCCTGC TCAACAACTA CATGATCTGG 
AACCTGGTGC GGAAAACAAG CTCCTTCCTT GACCAGCGCT TTCAGGACGC CGATGAGAAG 
TTCATGGAAG TCATGTACGG GACCAAGAAG ACCTGTCTTC CTCGCTGGAA GTTTTGCGTG 
AGTGACACAG AAAACAACCT GGGCTTTGCG TTGGGCCCCA TGTTTGTCAA AGCAACCTTC 
GCCGAGGACA GCAAGAGCAT AGCCACCGAG ATCATCCTGG AGATTAAGAA GGCATTTGAG 
GAAAGCCTGA GCACCCTGAA GTGGATGGAT GAGGAAACCC GAAAATCAGC CAAGGAAAAG 
GCCGATGCCA TCTACAACAT GATAGGATAC CCCAACTTCA TCATGGATCC CAAGGAGCTG 
GACAAAGTGT TTAATGACTA CACTGCAGTT CCAGACCTCT ACTTTGAAAA TGCCATGCGG 
TTTTTCAACT TCTCATGGAG GGTCACTGCC GATCAGCTCA GGAAAGCCCC CAACAGAGAT 
CAGTGGAGCA TGACCCCGCC CATGGTGAAC GCCTACTACT CGCCCACCAA GAATGAGATT 
GTGTTTCCGG CCGGGATCCT GCAGGCACCA TTCTACACAC GCTCCTCACC CAAGGCCTTA 
AACTTTGGTG GCATAGGTGT CGTCGTGGGC CATGAGCTGA CTCATGCTTT TGATGATCAA 
GGACGGGAGT ATGACAAGGA CGGGAACCTC CGGCCATGGT GGAAGAACTC ATCCGTGGAG 
GCCTTCAAGC GTCAGACCGA GTGCATGGTA GAGCAGTACA GCAACTACAG CGTGAACGGG 
GAGCCGGTGA ACGGGCGGCA CACCCTGGGG GAGAACATCG CCGACAACGG GGGTCTCAAG 
GCGGCCTATC GGGCTTACCA GAACTGGGTG AAGAAGAACG GGGCTGAGCA CTCGCTCCCC 
ACCCTGGGCC TCACCAATAA CCAGCTCTTC TTCCTGGGCT TTGCACAGGT CTGGTGCTCC 
GTCCGCACAC CTGAGAGCTC CCACGAAGGC CTCATCACCG ATCCCCACAG CCCCTCTCGC 
TTCCGGGTCA TCGGCTCCCT CTCCAATTCC AAGGAGTTCT CAGAACACTT CCGCTGCCCA 
CCTGGCTCAC CCATGAACCC GCCTCACAAG TGCGAAGTCT GGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ECE1 ( NM_001113348.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001106819.1 Homo sapiens endothelin converting enzyme 1 (ECE1), transcript variant 4, mRNA.

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CloneID	OHu19118
Clone ID Related Accession (Same CDS sequence)	NM_001113348.1 , NM_001113348.2
Accession Version	NM_001113348.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2265bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-03
Organism	Homo sapiens(human)
Product	endothelin-converting enzyme 1 isoform 4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB290492.1, AL031728.12, AB031742.1, AL031005.2, BC106022.1 and BU733112.1. This sequence is a reference standard in the RefSeqGene project. On May 31, 2019 this sequence version replaced NM_001113348.1. Transcript Variant: This variant (4) differs in the 5' UTR and coding region, compared to variant 1, resulting in a shorter protein (isoform 4 also known as isoform c) that has a distinct N-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.216561.1, SRR1660809.240261.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGATGTCGA CGTACAAGCG GGCCACGCTG GACGAGGAGG ACCTGGTGGA CTCGCTCTCC 
GAGGGCGACG CATACCCCAA CGGCCTGCAG GTGAACTTCC ACAGCCCCCG GAGTGGCCAG 
AGGTGCTGGG CTGCACGGAC CCAGGTGGAG AAGCGGCTGG TGGTGTTGGT GGTACTTCTG 
GCGGCAGGAC TGGTGGCCTG CTTGGCAGCA CTGGGCATCC AGTACCAGAC AAGATCCCCC 
TCTGTGTGCC TGAGCGAAGC TTGTGTCTCA GTGACCAGCT CCATCTTGAG CTCCATGGAC 
CCCACAGTGG ACCCCTGCCA TGACTTCTTC AGCTACGCCT GTGGGGGCTG GATCAAGGCC 
AACCCAGTCC CTGATGGCCA CTCACGCTGG GGGACCTTCA GCAACCTCTG GGAACACAAC 
CAAGCAATCA TCAAGCACCT CCTCGAAAAC TCCACGGCCA GCGTGAGCGA GGCAGAGAGA 
AAGGCGCAAG TATACTACCG TGCGTGCATG AACGAGACCA GGATCGAGGA GCTCAGGGCC 
AAACCTCTAA TGGAGTTGAT TGAGAGGCTC GGGGGCTGGA ACATCACAGG TCCCTGGGCC 
AAGGACAACT TCCAGGACAC CCTGCAGGTG GTCACCGCCC ACTACCGCAC CTCACCCTTC 
TTCTCTGTCT ATGTCAGTGC CGATTCCAAG AACTCCAACA GCAACGTGAT CCAGGTGGAC 
CAGTCTGGCC TGGGCTTGCC CTCGAGAGAC TATTACCTGA ACAAAACTGA AAACGAGAAG 
GTGCTGACCG GATATCTGAA CTACATGGTC CAGCTGGGGA AGCTGCTGGG CGGCGGGGAC 
GAGGAGGCCA TCCGGCCCCA GATGCAGCAG ATCTTGGACT TTGAGACGGC ACTGGCCAAC 
ATCACCATCC CACAGGAGAA GCGCCGTGAT GAGGAGCTCA TCTACCACAA AGTGACGGCA 
GCCGAGCTGC AGACCTTGGC ACCCGCCATC AACTGGTTGC CTTTTCTCAA CACCATCTTC 
TACCCCGTGG AGATCAATGA ATCCGAGCCT ATTGTGGTCT ATGACAAGGA ATACCTTGAG 
CAGATCTCCA CTCTCATCAA CACCACCGAC AGATGCCTGC TCAACAACTA CATGATCTGG 
AACCTGGTGC GGAAAACAAG CTCCTTCCTT GACCAGCGCT TTCAGGACGC CGATGAGAAG 
TTCATGGAAG TCATGTACGG GACCAAGAAG ACCTGTCTTC CTCGCTGGAA GTTTTGCGTG 
AGTGACACAG AAAACAACCT GGGCTTTGCG TTGGGCCCCA TGTTTGTCAA AGCAACCTTC 
GCCGAGGACA GCAAGAGCAT AGCCACCGAG ATCATCCTGG AGATTAAGAA GGCATTTGAG 
GAAAGCCTGA GCACCCTGAA GTGGATGGAT GAGGAAACCC GAAAATCAGC CAAGGAAAAG 
GCCGATGCCA TCTACAACAT GATAGGATAC CCCAACTTCA TCATGGATCC CAAGGAGCTG 
GACAAAGTGT TTAATGACTA CACTGCAGTT CCAGACCTCT ACTTTGAAAA TGCCATGCGG 
TTTTTCAACT TCTCATGGAG GGTCACTGCC GATCAGCTCA GGAAAGCCCC CAACAGAGAT 
CAGTGGAGCA TGACCCCGCC CATGGTGAAC GCCTACTACT CGCCCACCAA GAATGAGATT 
GTGTTTCCGG CCGGGATCCT GCAGGCACCA TTCTACACAC GCTCCTCACC CAAGGCCTTA 
AACTTTGGTG GCATAGGTGT CGTCGTGGGC CATGAGCTGA CTCATGCTTT TGATGATCAA 
GGACGGGAGT ATGACAAGGA CGGGAACCTC CGGCCATGGT GGAAGAACTC ATCCGTGGAG 
GCCTTCAAGC GTCAGACCGA GTGCATGGTA GAGCAGTACA GCAACTACAG CGTGAACGGG 
GAGCCGGTGA ACGGGCGGCA CACCCTGGGG GAGAACATCG CCGACAACGG GGGTCTCAAG 
GCGGCCTATC GGGCTTACCA GAACTGGGTG AAGAAGAACG GGGCTGAGCA CTCGCTCCCC 
ACCCTGGGCC TCACCAATAA CCAGCTCTTC TTCCTGGGCT TTGCACAGGT CTGGTGCTCC 
GTCCGCACAC CTGAGAGCTC CCACGAAGGC CTCATCACCG ATCCCCACAG CCCCTCTCGC 
TTCCGGGTCA TCGGCTCCCT CTCCAATTCC AAGGAGTTCT CAGAACACTT CCGCTGCCCA 
CCTGGCTCAC CCATGAACCC GCCTCACAAG TGCGAAGTCT GGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001106819.1
CDS	127..2391
Translation	MMSTYKRATLDEEDLVDSLSEGDAYPNGLQVNFHSPRSGQRCWAARTQVEKRLVVLVVLLAAGLVACLAALGIQYQTRSPSVCLSEACVSVTSSILSSMDPTVDPCHDFFSYACGGWIKANPVPDGHSRWGTFSNLWEHNQAIIKHLLENSTASVSEAERKAQVYYRACMNETRIEELRAKPLMELIERLGGWNITGPWAKDNFQDTLQVVTAHYRTSPFFSVYVSADSKNSNSNVIQVDQSGLGLPSRDYYLNKTENEKVLTGYLNYMVQLGKLLGGGDEEAIRPQMQQILDFETALANITIPQEKRRDEELIYHKVTAAELQTLAPAINWLPFLNTIFYPVEINESEPIVVYDKEYLEQISTLINTTDRCLLNNYMIWNLVRKTSSFLDQRFQDADEKFMEVMYGTKKTCLPRWKFCVSDTENNLGFALGPMFVKATFAEDSKSIATEIILEIKKAFEESLSTLKWMDEETRKSAKEKADAIYNMIGYPNFIMDPKELDKVFNDYTAVPDLYFENAMRFFNFSWRVTADQLRKAPNRDQWSMTPPMVNAYYSPTKNEIVFPAGILQAPFYTRSSPKALNFGGIGVVVGHELTHAFDDQGREYDKDGNLRPWWKNSSVEAFKRQTECMVEQYSNYSVNGEPVNGRHTLGENIADNGGLKAAYRAYQNWVKKNGAEHSLPTLGLTNNQLFFLGFAQVWCSVRTPESSHEGLITDPHSPSRFRVIGSLSNSKEFSEHFRCPPGSPMNPPHKCEVW

Target ORF information:

RefSeq Version	NM_001113348.2
Organism	Homo sapiens(human)
Definition	Homo sapiens endothelin converting enzyme 1 (ECE1), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001113348.2

ORF Insert Sequence:

ATGATGTCGA CGTACAAGCG GGCCACGCTG GACGAGGAGG ACCTGGTGGA CTCGCTCTCC 
GAGGGCGACG CATACCCCAA CGGCCTGCAG GTGAACTTCC ACAGCCCCCG GAGTGGCCAG 
AGGTGCTGGG CTGCACGGAC CCAGGTGGAG AAGCGGCTGG TGGTGTTGGT GGTACTTCTG 
GCGGCAGGAC TGGTGGCCTG CTTGGCAGCA CTGGGCATCC AGTACCAGAC AAGATCCCCC 
TCTGTGTGCC TGAGCGAAGC TTGTGTCTCA GTGACCAGCT CCATCTTGAG CTCCATGGAC 
CCCACAGTGG ACCCCTGCCA TGACTTCTTC AGCTACGCCT GTGGGGGCTG GATCAAGGCC 
AACCCAGTCC CTGATGGCCA CTCACGCTGG GGGACCTTCA GCAACCTCTG GGAACACAAC 
CAAGCAATCA TCAAGCACCT CCTCGAAAAC TCCACGGCCA GCGTGAGCGA GGCAGAGAGA 
AAGGCGCAAG TATACTACCG TGCGTGCATG AACGAGACCA GGATCGAGGA GCTCAGGGCC 
AAACCTCTAA TGGAGTTGAT TGAGAGGCTC GGGGGCTGGA ACATCACAGG TCCCTGGGCC 
AAGGACAACT TCCAGGACAC CCTGCAGGTG GTCACCGCCC ACTACCGCAC CTCACCCTTC 
TTCTCTGTCT ATGTCAGTGC CGATTCCAAG AACTCCAACA GCAACGTGAT CCAGGTGGAC 
CAGTCTGGCC TGGGCTTGCC CTCGAGAGAC TATTACCTGA ACAAAACTGA AAACGAGAAG 
GTGCTGACCG GATATCTGAA CTACATGGTC CAGCTGGGGA AGCTGCTGGG CGGCGGGGAC 
GAGGAGGCCA TCCGGCCCCA GATGCAGCAG ATCTTGGACT TTGAGACGGC ACTGGCCAAC 
ATCACCATCC CACAGGAGAA GCGCCGTGAT GAGGAGCTCA TCTACCACAA AGTGACGGCA 
GCCGAGCTGC AGACCTTGGC ACCCGCCATC AACTGGTTGC CTTTTCTCAA CACCATCTTC 
TACCCCGTGG AGATCAATGA ATCCGAGCCT ATTGTGGTCT ATGACAAGGA ATACCTTGAG 
CAGATCTCCA CTCTCATCAA CACCACCGAC AGATGCCTGC TCAACAACTA CATGATCTGG 
AACCTGGTGC GGAAAACAAG CTCCTTCCTT GACCAGCGCT TTCAGGACGC CGATGAGAAG 
TTCATGGAAG TCATGTACGG GACCAAGAAG ACCTGTCTTC CTCGCTGGAA GTTTTGCGTG 
AGTGACACAG AAAACAACCT GGGCTTTGCG TTGGGCCCCA TGTTTGTCAA AGCAACCTTC 
GCCGAGGACA GCAAGAGCAT AGCCACCGAG ATCATCCTGG AGATTAAGAA GGCATTTGAG 
GAAAGCCTGA GCACCCTGAA GTGGATGGAT GAGGAAACCC GAAAATCAGC CAAGGAAAAG 
GCCGATGCCA TCTACAACAT GATAGGATAC CCCAACTTCA TCATGGATCC CAAGGAGCTG 
GACAAAGTGT TTAATGACTA CACTGCAGTT CCAGACCTCT ACTTTGAAAA TGCCATGCGG 
TTTTTCAACT TCTCATGGAG GGTCACTGCC GATCAGCTCA GGAAAGCCCC CAACAGAGAT 
CAGTGGAGCA TGACCCCGCC CATGGTGAAC GCCTACTACT CGCCCACCAA GAATGAGATT 
GTGTTTCCGG CCGGGATCCT GCAGGCACCA TTCTACACAC GCTCCTCACC CAAGGCCTTA 
AACTTTGGTG GCATAGGTGT CGTCGTGGGC CATGAGCTGA CTCATGCTTT TGATGATCAA 
GGACGGGAGT ATGACAAGGA CGGGAACCTC CGGCCATGGT GGAAGAACTC ATCCGTGGAG 
GCCTTCAAGC GTCAGACCGA GTGCATGGTA GAGCAGTACA GCAACTACAG CGTGAACGGG 
GAGCCGGTGA ACGGGCGGCA CACCCTGGGG GAGAACATCG CCGACAACGG GGGTCTCAAG 
GCGGCCTATC GGGCTTACCA GAACTGGGTG AAGAAGAACG GGGCTGAGCA CTCGCTCCCC 
ACCCTGGGCC TCACCAATAA CCAGCTCTTC TTCCTGGGCT TTGCACAGGT CTGGTGCTCC 
GTCCGCACAC CTGAGAGCTC CCACGAAGGC CTCATCACCG ATCCCCACAG CCCCTCTCGC 
TTCCGGGTCA TCGGCTCCCT CTCCAATTCC AAGGAGTTCT CAGAACACTT CCGCTGCCCA 
CCTGGCTCAC CCATGAACCC GCCTCACAAG TGCGAAGTCT GGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ECE1 ( XM_017000511.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016856000.1 Homo sapiens endothelin converting enzyme 1 (ECE1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu33089
Clone ID Related Accession (Same CDS sequence)	XM_017000511.1 , XM_011540873.2 , XM_006710398.2
Accession Version	XM_017000511.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2262bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	endothelin-converting enzyme 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGACGT ACAAGCGGGC CACGCTGGAC GAGGAGGACC TGGTGGACTC GCTCTCCGAG 
GGCGACGCAT ACCCCAACGG CCTGCAGGTG AACTTCCACA GCCCCCGGAG TGGCCAGAGG 
TGCTGGGCTG CACGGACCCA GGTGGAGAAG CGGCTGGTGG TGTTGGTGGT ACTTCTGGCG 
GCAGGACTGG TGGCCTGCTT GGCAGCACTG GGCATCCAGT ACCAGACAAG ATCCCCCTCT 
GTGTGCCTGA GCGAAGCTTG TGTCTCAGTG ACCAGCTCCA TCTTGAGCTC CATGGACCCC 
ACAGTGGACC CCTGCCATGA CTTCTTCAGC TACGCCTGTG GGGGCTGGAT CAAGGCCAAC 
CCAGTCCCTG ATGGCCACTC ACGCTGGGGG ACCTTCAGCA ACCTCTGGGA ACACAACCAA 
GCAATCATCA AGCACCTCCT CGAAAACTCC ACGGCCAGCG TGAGCGAGGC AGAGAGAAAG 
GCGCAAGTAT ACTACCGTGC GTGCATGAAC GAGACCAGGA TCGAGGAGCT CAGGGCCAAA 
CCTCTAATGG AGTTGATTGA GAGGCTCGGG GGCTGGAACA TCACAGGTCC CTGGGCCAAG 
GACAACTTCC AGGACACCCT GCAGGTGGTC ACCGCCCACT ACCGCACCTC ACCCTTCTTC 
TCTGTCTATG TCAGTGCCGA TTCCAAGAAC TCCAACAGCA ACGTGATCCA GGTGGACCAG 
TCTGGCCTGG GCTTGCCCTC GAGAGACTAT TACCTGAACA AAACTGAAAA CGAGAAGGTG 
CTGACCGGAT ATCTGAACTA CATGGTCCAG CTGGGGAAGC TGCTGGGCGG CGGGGACGAG 
GAGGCCATCC GGCCCCAGAT GCAGCAGATC TTGGACTTTG AGACGGCACT GGCCAACATC 
ACCATCCCAC AGGAGAAGCG CCGTGATGAG GAGCTCATCT ACCACAAAGT GACGGCAGCC 
GAGCTGCAGA CCTTGGCACC CGCCATCAAC TGGTTGCCTT TTCTCAACAC CATCTTCTAC 
CCCGTGGAGA TCAATGAATC CGAGCCTATT GTGGTCTATG ACAAGGAATA CCTTGAGCAG 
ATCTCCACTC TCATCAACAC CACCGACAGA TGCCTGCTCA ACAACTACAT GATCTGGAAC 
CTGGTGCGGA AAACAAGCTC CTTCCTTGAC CAGCGCTTTC AGGACGCCGA TGAGAAGTTC 
ATGGAAGTCA TGTACGGGAC CAAGAAGACC TGTCTTCCTC GCTGGAAGTT TTGCGTGAGT 
GACACAGAAA ACAACCTGGG CTTTGCGTTG GGCCCCATGT TTGTCAAAGC AACCTTCGCC 
GAGGACAGCA AGAGCATAGC CACCGAGATC ATCCTGGAGA TTAAGAAGGC ATTTGAGGAA 
AGCCTGAGCA CCCTGAAGTG GATGGATGAG GAAACCCGAA AATCAGCCAA GGAAAAGGCC 
GATGCCATCT ACAACATGAT AGGATACCCC AACTTCATCA TGGATCCCAA GGAGCTGGAC 
AAAGTGTTTA ATGACTACAC TGCAGTTCCA GACCTCTACT TTGAAAATGC CATGCGGTTT 
TTCAACTTCT CATGGAGGGT CACTGCCGAT CAGCTCAGGA AAGCCCCCAA CAGAGATCAG 
TGGAGCATGA CCCCGCCCAT GGTGAACGCC TACTACTCGC CCACCAAGAA TGAGATTGTG 
TTTCCGGCCG GGATCCTGCA GGCACCATTC TACACACGCT CCTCACCCAA GGCCTTAAAC 
TTTGGTGGCA TAGGTGTCGT CGTGGGCCAT GAGCTGACTC ATGCTTTTGA TGATCAAGGA 
CGGGAGTATG ACAAGGACGG GAACCTCCGG CCATGGTGGA AGAACTCATC CGTGGAGGCC 
TTCAAGCGTC AGACCGAGTG CATGGTAGAG CAGTACAGCA ACTACAGCGT GAACGGGGAG 
CCGGTGAACG GGCGGCACAC CCTGGGGGAG AACATCGCCG ACAACGGGGG TCTCAAGGCG 
GCCTATCGGG CTTACCAGAA CTGGGTGAAG AAGAACGGGG CTGAGCACTC GCTCCCCACC 
CTGGGCCTCA CCAATAACCA GCTCTTCTTC CTGGGCTTTG CACAGGTCTG GTGCTCCGTC 
CGCACACCTG AGAGCTCCCA CGAAGGCCTC ATCACCGATC CCCACAGCCC CTCTCGCTTC 
CGGGTCATCG GCTCCCTCTC CAATTCCAAG GAGTTCTCAG AACACTTCCG CTGCCCACCT 
GGCTCACCCA TGAACCCGCC TCACAAGTGC GAAGTCTGGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016856000.1
CDS	19..2280
Translation	MSTYKRATLDEEDLVDSLSEGDAYPNGLQVNFHSPRSGQRCWAARTQVEKRLVVLVVLLAAGLVACLAALGIQYQTRSPSVCLSEACVSVTSSILSSMDPTVDPCHDFFSYACGGWIKANPVPDGHSRWGTFSNLWEHNQAIIKHLLENSTASVSEAERKAQVYYRACMNETRIEELRAKPLMELIERLGGWNITGPWAKDNFQDTLQVVTAHYRTSPFFSVYVSADSKNSNSNVIQVDQSGLGLPSRDYYLNKTENEKVLTGYLNYMVQLGKLLGGGDEEAIRPQMQQILDFETALANITIPQEKRRDEELIYHKVTAAELQTLAPAINWLPFLNTIFYPVEINESEPIVVYDKEYLEQISTLINTTDRCLLNNYMIWNLVRKTSSFLDQRFQDADEKFMEVMYGTKKTCLPRWKFCVSDTENNLGFALGPMFVKATFAEDSKSIATEIILEIKKAFEESLSTLKWMDEETRKSAKEKADAIYNMIGYPNFIMDPKELDKVFNDYTAVPDLYFENAMRFFNFSWRVTADQLRKAPNRDQWSMTPPMVNAYYSPTKNEIVFPAGILQAPFYTRSSPKALNFGGIGVVVGHELTHAFDDQGREYDKDGNLRPWWKNSSVEAFKRQTECMVEQYSNYSVNGEPVNGRHTLGENIADNGGLKAAYRAYQNWVKKNGAEHSLPTLGLTNNQLFFLGFAQVWCSVRTPESSHEGLITDPHSPSRFRVIGSLSNSKEFSEHFRCPPGSPMNPPHKCEVW

Target ORF information:

RefSeq Version	XM_017000511.1
Organism	Homo sapiens(human)
Definition	Homo sapiens endothelin converting enzyme 1 (ECE1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017000511.1

ORF Insert Sequence:

ATGTCGACGT ACAAGCGGGC CACGCTGGAC GAGGAGGACC TGGTGGACTC GCTCTCCGAG 
GGCGACGCAT ACCCCAACGG CCTGCAGGTG AACTTCCACA GCCCCCGGAG TGGCCAGAGG 
TGCTGGGCTG CACGGACCCA GGTGGAGAAG CGGCTGGTGG TGTTGGTGGT ACTTCTGGCG 
GCAGGACTGG TGGCCTGCTT GGCAGCACTG GGCATCCAGT ACCAGACAAG ATCCCCCTCT 
GTGTGCCTGA GCGAAGCTTG TGTCTCAGTG ACCAGCTCCA TCTTGAGCTC CATGGACCCC 
ACAGTGGACC CCTGCCATGA CTTCTTCAGC TACGCCTGTG GGGGCTGGAT CAAGGCCAAC 
CCAGTCCCTG ATGGCCACTC ACGCTGGGGG ACCTTCAGCA ACCTCTGGGA ACACAACCAA 
GCAATCATCA AGCACCTCCT CGAAAACTCC ACGGCCAGCG TGAGCGAGGC AGAGAGAAAG 
GCGCAAGTAT ACTACCGTGC GTGCATGAAC GAGACCAGGA TCGAGGAGCT CAGGGCCAAA 
CCTCTAATGG AGTTGATTGA GAGGCTCGGG GGCTGGAACA TCACAGGTCC CTGGGCCAAG 
GACAACTTCC AGGACACCCT GCAGGTGGTC ACCGCCCACT ACCGCACCTC ACCCTTCTTC 
TCTGTCTATG TCAGTGCCGA TTCCAAGAAC TCCAACAGCA ACGTGATCCA GGTGGACCAG 
TCTGGCCTGG GCTTGCCCTC GAGAGACTAT TACCTGAACA AAACTGAAAA CGAGAAGGTG 
CTGACCGGAT ATCTGAACTA CATGGTCCAG CTGGGGAAGC TGCTGGGCGG CGGGGACGAG 
GAGGCCATCC GGCCCCAGAT GCAGCAGATC TTGGACTTTG AGACGGCACT GGCCAACATC 
ACCATCCCAC AGGAGAAGCG CCGTGATGAG GAGCTCATCT ACCACAAAGT GACGGCAGCC 
GAGCTGCAGA CCTTGGCACC CGCCATCAAC TGGTTGCCTT TTCTCAACAC CATCTTCTAC 
CCCGTGGAGA TCAATGAATC CGAGCCTATT GTGGTCTATG ACAAGGAATA CCTTGAGCAG 
ATCTCCACTC TCATCAACAC CACCGACAGA TGCCTGCTCA ACAACTACAT GATCTGGAAC 
CTGGTGCGGA AAACAAGCTC CTTCCTTGAC CAGCGCTTTC AGGACGCCGA TGAGAAGTTC 
ATGGAAGTCA TGTACGGGAC CAAGAAGACC TGTCTTCCTC GCTGGAAGTT TTGCGTGAGT 
GACACAGAAA ACAACCTGGG CTTTGCGTTG GGCCCCATGT TTGTCAAAGC AACCTTCGCC 
GAGGACAGCA AGAGCATAGC CACCGAGATC ATCCTGGAGA TTAAGAAGGC ATTTGAGGAA 
AGCCTGAGCA CCCTGAAGTG GATGGATGAG GAAACCCGAA AATCAGCCAA GGAAAAGGCC 
GATGCCATCT ACAACATGAT AGGATACCCC AACTTCATCA TGGATCCCAA GGAGCTGGAC 
AAAGTGTTTA ATGACTACAC TGCAGTTCCA GACCTCTACT TTGAAAATGC CATGCGGTTT 
TTCAACTTCT CATGGAGGGT CACTGCCGAT CAGCTCAGGA AAGCCCCCAA CAGAGATCAG 
TGGAGCATGA CCCCGCCCAT GGTGAACGCC TACTACTCGC CCACCAAGAA TGAGATTGTG 
TTTCCGGCCG GGATCCTGCA GGCACCATTC TACACACGCT CCTCACCCAA GGCCTTAAAC 
TTTGGTGGCA TAGGTGTCGT CGTGGGCCAT GAGCTGACTC ATGCTTTTGA TGATCAAGGA 
CGGGAGTATG ACAAGGACGG GAACCTCCGG CCATGGTGGA AGAACTCATC CGTGGAGGCC 
TTCAAGCGTC AGACCGAGTG CATGGTAGAG CAGTACAGCA ACTACAGCGT GAACGGGGAG 
CCGGTGAACG GGCGGCACAC CCTGGGGGAG AACATCGCCG ACAACGGGGG TCTCAAGGCG 
GCCTATCGGG CTTACCAGAA CTGGGTGAAG AAGAACGGGG CTGAGCACTC GCTCCCCACC 
CTGGGCCTCA CCAATAACCA GCTCTTCTTC CTGGGCTTTG CACAGGTCTG GTGCTCCGTC 
CGCACACCTG AGAGCTCCCA CGAAGGCCTC ATCACCGATC CCCACAGCCC CTCTCGCTTC 
CGGGTCATCG GCTCCCTCTC CAATTCCAAG GAGTTCTCAG AACACTTCCG CTGCCCACCT 
GGCTCACCCA TGAACCCGCC TCACAAGTGC GAAGTCTGGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ECE1 ( XM_011540873.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011539175.1 Homo sapiens endothelin converting enzyme 1 (ECE1), transcript variant X3, mRNA.

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CloneID	OHu33089
Clone ID Related Accession (Same CDS sequence)	XM_017000511.1 , XM_011540873.2 , XM_006710398.2
Accession Version	XM_011540873.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2262bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	endothelin-converting enzyme 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011540873.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGACGT ACAAGCGGGC CACGCTGGAC GAGGAGGACC TGGTGGACTC GCTCTCCGAG 
GGCGACGCAT ACCCCAACGG CCTGCAGGTG AACTTCCACA GCCCCCGGAG TGGCCAGAGG 
TGCTGGGCTG CACGGACCCA GGTGGAGAAG CGGCTGGTGG TGTTGGTGGT ACTTCTGGCG 
GCAGGACTGG TGGCCTGCTT GGCAGCACTG GGCATCCAGT ACCAGACAAG ATCCCCCTCT 
GTGTGCCTGA GCGAAGCTTG TGTCTCAGTG ACCAGCTCCA TCTTGAGCTC CATGGACCCC 
ACAGTGGACC CCTGCCATGA CTTCTTCAGC TACGCCTGTG GGGGCTGGAT CAAGGCCAAC 
CCAGTCCCTG ATGGCCACTC ACGCTGGGGG ACCTTCAGCA ACCTCTGGGA ACACAACCAA 
GCAATCATCA AGCACCTCCT CGAAAACTCC ACGGCCAGCG TGAGCGAGGC AGAGAGAAAG 
GCGCAAGTAT ACTACCGTGC GTGCATGAAC GAGACCAGGA TCGAGGAGCT CAGGGCCAAA 
CCTCTAATGG AGTTGATTGA GAGGCTCGGG GGCTGGAACA TCACAGGTCC CTGGGCCAAG 
GACAACTTCC AGGACACCCT GCAGGTGGTC ACCGCCCACT ACCGCACCTC ACCCTTCTTC 
TCTGTCTATG TCAGTGCCGA TTCCAAGAAC TCCAACAGCA ACGTGATCCA GGTGGACCAG 
TCTGGCCTGG GCTTGCCCTC GAGAGACTAT TACCTGAACA AAACTGAAAA CGAGAAGGTG 
CTGACCGGAT ATCTGAACTA CATGGTCCAG CTGGGGAAGC TGCTGGGCGG CGGGGACGAG 
GAGGCCATCC GGCCCCAGAT GCAGCAGATC TTGGACTTTG AGACGGCACT GGCCAACATC 
ACCATCCCAC AGGAGAAGCG CCGTGATGAG GAGCTCATCT ACCACAAAGT GACGGCAGCC 
GAGCTGCAGA CCTTGGCACC CGCCATCAAC TGGTTGCCTT TTCTCAACAC CATCTTCTAC 
CCCGTGGAGA TCAATGAATC CGAGCCTATT GTGGTCTATG ACAAGGAATA CCTTGAGCAG 
ATCTCCACTC TCATCAACAC CACCGACAGA TGCCTGCTCA ACAACTACAT GATCTGGAAC 
CTGGTGCGGA AAACAAGCTC CTTCCTTGAC CAGCGCTTTC AGGACGCCGA TGAGAAGTTC 
ATGGAAGTCA TGTACGGGAC CAAGAAGACC TGTCTTCCTC GCTGGAAGTT TTGCGTGAGT 
GACACAGAAA ACAACCTGGG CTTTGCGTTG GGCCCCATGT TTGTCAAAGC AACCTTCGCC 
GAGGACAGCA AGAGCATAGC CACCGAGATC ATCCTGGAGA TTAAGAAGGC ATTTGAGGAA 
AGCCTGAGCA CCCTGAAGTG GATGGATGAG GAAACCCGAA AATCAGCCAA GGAAAAGGCC 
GATGCCATCT ACAACATGAT AGGATACCCC AACTTCATCA TGGATCCCAA GGAGCTGGAC 
AAAGTGTTTA ATGACTACAC TGCAGTTCCA GACCTCTACT TTGAAAATGC CATGCGGTTT 
TTCAACTTCT CATGGAGGGT CACTGCCGAT CAGCTCAGGA AAGCCCCCAA CAGAGATCAG 
TGGAGCATGA CCCCGCCCAT GGTGAACGCC TACTACTCGC CCACCAAGAA TGAGATTGTG 
TTTCCGGCCG GGATCCTGCA GGCACCATTC TACACACGCT CCTCACCCAA GGCCTTAAAC 
TTTGGTGGCA TAGGTGTCGT CGTGGGCCAT GAGCTGACTC ATGCTTTTGA TGATCAAGGA 
CGGGAGTATG ACAAGGACGG GAACCTCCGG CCATGGTGGA AGAACTCATC CGTGGAGGCC 
TTCAAGCGTC AGACCGAGTG CATGGTAGAG CAGTACAGCA ACTACAGCGT GAACGGGGAG 
CCGGTGAACG GGCGGCACAC CCTGGGGGAG AACATCGCCG ACAACGGGGG TCTCAAGGCG 
GCCTATCGGG CTTACCAGAA CTGGGTGAAG AAGAACGGGG CTGAGCACTC GCTCCCCACC 
CTGGGCCTCA CCAATAACCA GCTCTTCTTC CTGGGCTTTG CACAGGTCTG GTGCTCCGTC 
CGCACACCTG AGAGCTCCCA CGAAGGCCTC ATCACCGATC CCCACAGCCC CTCTCGCTTC 
CGGGTCATCG GCTCCCTCTC CAATTCCAAG GAGTTCTCAG AACACTTCCG CTGCCCACCT 
GGCTCACCCA TGAACCCGCC TCACAAGTGC GAAGTCTGGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011539175.1
CDS	197..2458
Translation	MSTYKRATLDEEDLVDSLSEGDAYPNGLQVNFHSPRSGQRCWAARTQVEKRLVVLVVLLAAGLVACLAALGIQYQTRSPSVCLSEACVSVTSSILSSMDPTVDPCHDFFSYACGGWIKANPVPDGHSRWGTFSNLWEHNQAIIKHLLENSTASVSEAERKAQVYYRACMNETRIEELRAKPLMELIERLGGWNITGPWAKDNFQDTLQVVTAHYRTSPFFSVYVSADSKNSNSNVIQVDQSGLGLPSRDYYLNKTENEKVLTGYLNYMVQLGKLLGGGDEEAIRPQMQQILDFETALANITIPQEKRRDEELIYHKVTAAELQTLAPAINWLPFLNTIFYPVEINESEPIVVYDKEYLEQISTLINTTDRCLLNNYMIWNLVRKTSSFLDQRFQDADEKFMEVMYGTKKTCLPRWKFCVSDTENNLGFALGPMFVKATFAEDSKSIATEIILEIKKAFEESLSTLKWMDEETRKSAKEKADAIYNMIGYPNFIMDPKELDKVFNDYTAVPDLYFENAMRFFNFSWRVTADQLRKAPNRDQWSMTPPMVNAYYSPTKNEIVFPAGILQAPFYTRSSPKALNFGGIGVVVGHELTHAFDDQGREYDKDGNLRPWWKNSSVEAFKRQTECMVEQYSNYSVNGEPVNGRHTLGENIADNGGLKAAYRAYQNWVKKNGAEHSLPTLGLTNNQLFFLGFAQVWCSVRTPESSHEGLITDPHSPSRFRVIGSLSNSKEFSEHFRCPPGSPMNPPHKCEVW

Target ORF information:

RefSeq Version	XM_011540873.2
Organism	Homo sapiens(human)
Definition	Homo sapiens endothelin converting enzyme 1 (ECE1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011540873.2

ORF Insert Sequence:

ATGTCGACGT ACAAGCGGGC CACGCTGGAC GAGGAGGACC TGGTGGACTC GCTCTCCGAG 
GGCGACGCAT ACCCCAACGG CCTGCAGGTG AACTTCCACA GCCCCCGGAG TGGCCAGAGG 
TGCTGGGCTG CACGGACCCA GGTGGAGAAG CGGCTGGTGG TGTTGGTGGT ACTTCTGGCG 
GCAGGACTGG TGGCCTGCTT GGCAGCACTG GGCATCCAGT ACCAGACAAG ATCCCCCTCT 
GTGTGCCTGA GCGAAGCTTG TGTCTCAGTG ACCAGCTCCA TCTTGAGCTC CATGGACCCC 
ACAGTGGACC CCTGCCATGA CTTCTTCAGC TACGCCTGTG GGGGCTGGAT CAAGGCCAAC 
CCAGTCCCTG ATGGCCACTC ACGCTGGGGG ACCTTCAGCA ACCTCTGGGA ACACAACCAA 
GCAATCATCA AGCACCTCCT CGAAAACTCC ACGGCCAGCG TGAGCGAGGC AGAGAGAAAG 
GCGCAAGTAT ACTACCGTGC GTGCATGAAC GAGACCAGGA TCGAGGAGCT CAGGGCCAAA 
CCTCTAATGG AGTTGATTGA GAGGCTCGGG GGCTGGAACA TCACAGGTCC CTGGGCCAAG 
GACAACTTCC AGGACACCCT GCAGGTGGTC ACCGCCCACT ACCGCACCTC ACCCTTCTTC 
TCTGTCTATG TCAGTGCCGA TTCCAAGAAC TCCAACAGCA ACGTGATCCA GGTGGACCAG 
TCTGGCCTGG GCTTGCCCTC GAGAGACTAT TACCTGAACA AAACTGAAAA CGAGAAGGTG 
CTGACCGGAT ATCTGAACTA CATGGTCCAG CTGGGGAAGC TGCTGGGCGG CGGGGACGAG 
GAGGCCATCC GGCCCCAGAT GCAGCAGATC TTGGACTTTG AGACGGCACT GGCCAACATC 
ACCATCCCAC AGGAGAAGCG CCGTGATGAG GAGCTCATCT ACCACAAAGT GACGGCAGCC 
GAGCTGCAGA CCTTGGCACC CGCCATCAAC TGGTTGCCTT TTCTCAACAC CATCTTCTAC 
CCCGTGGAGA TCAATGAATC CGAGCCTATT GTGGTCTATG ACAAGGAATA CCTTGAGCAG 
ATCTCCACTC TCATCAACAC CACCGACAGA TGCCTGCTCA ACAACTACAT GATCTGGAAC 
CTGGTGCGGA AAACAAGCTC CTTCCTTGAC CAGCGCTTTC AGGACGCCGA TGAGAAGTTC 
ATGGAAGTCA TGTACGGGAC CAAGAAGACC TGTCTTCCTC GCTGGAAGTT TTGCGTGAGT 
GACACAGAAA ACAACCTGGG CTTTGCGTTG GGCCCCATGT TTGTCAAAGC AACCTTCGCC 
GAGGACAGCA AGAGCATAGC CACCGAGATC ATCCTGGAGA TTAAGAAGGC ATTTGAGGAA 
AGCCTGAGCA CCCTGAAGTG GATGGATGAG GAAACCCGAA AATCAGCCAA GGAAAAGGCC 
GATGCCATCT ACAACATGAT AGGATACCCC AACTTCATCA TGGATCCCAA GGAGCTGGAC 
AAAGTGTTTA ATGACTACAC TGCAGTTCCA GACCTCTACT TTGAAAATGC CATGCGGTTT 
TTCAACTTCT CATGGAGGGT CACTGCCGAT CAGCTCAGGA AAGCCCCCAA CAGAGATCAG 
TGGAGCATGA CCCCGCCCAT GGTGAACGCC TACTACTCGC CCACCAAGAA TGAGATTGTG 
TTTCCGGCCG GGATCCTGCA GGCACCATTC TACACACGCT CCTCACCCAA GGCCTTAAAC 
TTTGGTGGCA TAGGTGTCGT CGTGGGCCAT GAGCTGACTC ATGCTTTTGA TGATCAAGGA 
CGGGAGTATG ACAAGGACGG GAACCTCCGG CCATGGTGGA AGAACTCATC CGTGGAGGCC 
TTCAAGCGTC AGACCGAGTG CATGGTAGAG CAGTACAGCA ACTACAGCGT GAACGGGGAG 
CCGGTGAACG GGCGGCACAC CCTGGGGGAG AACATCGCCG ACAACGGGGG TCTCAAGGCG 
GCCTATCGGG CTTACCAGAA CTGGGTGAAG AAGAACGGGG CTGAGCACTC GCTCCCCACC 
CTGGGCCTCA CCAATAACCA GCTCTTCTTC CTGGGCTTTG CACAGGTCTG GTGCTCCGTC 
CGCACACCTG AGAGCTCCCA CGAAGGCCTC ATCACCGATC CCCACAGCCC CTCTCGCTTC 
CGGGTCATCG GCTCCCTCTC CAATTCCAAG GAGTTCTCAG AACACTTCCG CTGCCCACCT 
GGCTCACCCA TGAACCCGCC TCACAAGTGC GAAGTCTGGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ECE1 ( XM_006710398.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_006710461.1 Homo sapiens endothelin converting enzyme 1 (ECE1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu33089
Clone ID Related Accession (Same CDS sequence)	XM_017000511.1 , XM_011540873.2 , XM_006710398.2
Accession Version	XM_006710398.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2262bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	endothelin-converting enzyme 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006710398.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGACGT ACAAGCGGGC CACGCTGGAC GAGGAGGACC TGGTGGACTC GCTCTCCGAG 
GGCGACGCAT ACCCCAACGG CCTGCAGGTG AACTTCCACA GCCCCCGGAG TGGCCAGAGG 
TGCTGGGCTG CACGGACCCA GGTGGAGAAG CGGCTGGTGG TGTTGGTGGT ACTTCTGGCG 
GCAGGACTGG TGGCCTGCTT GGCAGCACTG GGCATCCAGT ACCAGACAAG ATCCCCCTCT 
GTGTGCCTGA GCGAAGCTTG TGTCTCAGTG ACCAGCTCCA TCTTGAGCTC CATGGACCCC 
ACAGTGGACC CCTGCCATGA CTTCTTCAGC TACGCCTGTG GGGGCTGGAT CAAGGCCAAC 
CCAGTCCCTG ATGGCCACTC ACGCTGGGGG ACCTTCAGCA ACCTCTGGGA ACACAACCAA 
GCAATCATCA AGCACCTCCT CGAAAACTCC ACGGCCAGCG TGAGCGAGGC AGAGAGAAAG 
GCGCAAGTAT ACTACCGTGC GTGCATGAAC GAGACCAGGA TCGAGGAGCT CAGGGCCAAA 
CCTCTAATGG AGTTGATTGA GAGGCTCGGG GGCTGGAACA TCACAGGTCC CTGGGCCAAG 
GACAACTTCC AGGACACCCT GCAGGTGGTC ACCGCCCACT ACCGCACCTC ACCCTTCTTC 
TCTGTCTATG TCAGTGCCGA TTCCAAGAAC TCCAACAGCA ACGTGATCCA GGTGGACCAG 
TCTGGCCTGG GCTTGCCCTC GAGAGACTAT TACCTGAACA AAACTGAAAA CGAGAAGGTG 
CTGACCGGAT ATCTGAACTA CATGGTCCAG CTGGGGAAGC TGCTGGGCGG CGGGGACGAG 
GAGGCCATCC GGCCCCAGAT GCAGCAGATC TTGGACTTTG AGACGGCACT GGCCAACATC 
ACCATCCCAC AGGAGAAGCG CCGTGATGAG GAGCTCATCT ACCACAAAGT GACGGCAGCC 
GAGCTGCAGA CCTTGGCACC CGCCATCAAC TGGTTGCCTT TTCTCAACAC CATCTTCTAC 
CCCGTGGAGA TCAATGAATC CGAGCCTATT GTGGTCTATG ACAAGGAATA CCTTGAGCAG 
ATCTCCACTC TCATCAACAC CACCGACAGA TGCCTGCTCA ACAACTACAT GATCTGGAAC 
CTGGTGCGGA AAACAAGCTC CTTCCTTGAC CAGCGCTTTC AGGACGCCGA TGAGAAGTTC 
ATGGAAGTCA TGTACGGGAC CAAGAAGACC TGTCTTCCTC GCTGGAAGTT TTGCGTGAGT 
GACACAGAAA ACAACCTGGG CTTTGCGTTG GGCCCCATGT TTGTCAAAGC AACCTTCGCC 
GAGGACAGCA AGAGCATAGC CACCGAGATC ATCCTGGAGA TTAAGAAGGC ATTTGAGGAA 
AGCCTGAGCA CCCTGAAGTG GATGGATGAG GAAACCCGAA AATCAGCCAA GGAAAAGGCC 
GATGCCATCT ACAACATGAT AGGATACCCC AACTTCATCA TGGATCCCAA GGAGCTGGAC 
AAAGTGTTTA ATGACTACAC TGCAGTTCCA GACCTCTACT TTGAAAATGC CATGCGGTTT 
TTCAACTTCT CATGGAGGGT CACTGCCGAT CAGCTCAGGA AAGCCCCCAA CAGAGATCAG 
TGGAGCATGA CCCCGCCCAT GGTGAACGCC TACTACTCGC CCACCAAGAA TGAGATTGTG 
TTTCCGGCCG GGATCCTGCA GGCACCATTC TACACACGCT CCTCACCCAA GGCCTTAAAC 
TTTGGTGGCA TAGGTGTCGT CGTGGGCCAT GAGCTGACTC ATGCTTTTGA TGATCAAGGA 
CGGGAGTATG ACAAGGACGG GAACCTCCGG CCATGGTGGA AGAACTCATC CGTGGAGGCC 
TTCAAGCGTC AGACCGAGTG CATGGTAGAG CAGTACAGCA ACTACAGCGT GAACGGGGAG 
CCGGTGAACG GGCGGCACAC CCTGGGGGAG AACATCGCCG ACAACGGGGG TCTCAAGGCG 
GCCTATCGGG CTTACCAGAA CTGGGTGAAG AAGAACGGGG CTGAGCACTC GCTCCCCACC 
CTGGGCCTCA CCAATAACCA GCTCTTCTTC CTGGGCTTTG CACAGGTCTG GTGCTCCGTC 
CGCACACCTG AGAGCTCCCA CGAAGGCCTC ATCACCGATC CCCACAGCCC CTCTCGCTTC 
CGGGTCATCG GCTCCCTCTC CAATTCCAAG GAGTTCTCAG AACACTTCCG CTGCCCACCT 
GGCTCACCCA TGAACCCGCC TCACAAGTGC GAAGTCTGGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006710461.1
CDS	272..2533
Translation	MSTYKRATLDEEDLVDSLSEGDAYPNGLQVNFHSPRSGQRCWAARTQVEKRLVVLVVLLAAGLVACLAALGIQYQTRSPSVCLSEACVSVTSSILSSMDPTVDPCHDFFSYACGGWIKANPVPDGHSRWGTFSNLWEHNQAIIKHLLENSTASVSEAERKAQVYYRACMNETRIEELRAKPLMELIERLGGWNITGPWAKDNFQDTLQVVTAHYRTSPFFSVYVSADSKNSNSNVIQVDQSGLGLPSRDYYLNKTENEKVLTGYLNYMVQLGKLLGGGDEEAIRPQMQQILDFETALANITIPQEKRRDEELIYHKVTAAELQTLAPAINWLPFLNTIFYPVEINESEPIVVYDKEYLEQISTLINTTDRCLLNNYMIWNLVRKTSSFLDQRFQDADEKFMEVMYGTKKTCLPRWKFCVSDTENNLGFALGPMFVKATFAEDSKSIATEIILEIKKAFEESLSTLKWMDEETRKSAKEKADAIYNMIGYPNFIMDPKELDKVFNDYTAVPDLYFENAMRFFNFSWRVTADQLRKAPNRDQWSMTPPMVNAYYSPTKNEIVFPAGILQAPFYTRSSPKALNFGGIGVVVGHELTHAFDDQGREYDKDGNLRPWWKNSSVEAFKRQTECMVEQYSNYSVNGEPVNGRHTLGENIADNGGLKAAYRAYQNWVKKNGAEHSLPTLGLTNNQLFFLGFAQVWCSVRTPESSHEGLITDPHSPSRFRVIGSLSNSKEFSEHFRCPPGSPMNPPHKCEVW

Target ORF information:

RefSeq Version	XM_006710398.2
Organism	Homo sapiens(human)
Definition	Homo sapiens endothelin converting enzyme 1 (ECE1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006710398.2

ORF Insert Sequence:

ATGTCGACGT ACAAGCGGGC CACGCTGGAC GAGGAGGACC TGGTGGACTC GCTCTCCGAG 
GGCGACGCAT ACCCCAACGG CCTGCAGGTG AACTTCCACA GCCCCCGGAG TGGCCAGAGG 
TGCTGGGCTG CACGGACCCA GGTGGAGAAG CGGCTGGTGG TGTTGGTGGT ACTTCTGGCG 
GCAGGACTGG TGGCCTGCTT GGCAGCACTG GGCATCCAGT ACCAGACAAG ATCCCCCTCT 
GTGTGCCTGA GCGAAGCTTG TGTCTCAGTG ACCAGCTCCA TCTTGAGCTC CATGGACCCC 
ACAGTGGACC CCTGCCATGA CTTCTTCAGC TACGCCTGTG GGGGCTGGAT CAAGGCCAAC 
CCAGTCCCTG ATGGCCACTC ACGCTGGGGG ACCTTCAGCA ACCTCTGGGA ACACAACCAA 
GCAATCATCA AGCACCTCCT CGAAAACTCC ACGGCCAGCG TGAGCGAGGC AGAGAGAAAG 
GCGCAAGTAT ACTACCGTGC GTGCATGAAC GAGACCAGGA TCGAGGAGCT CAGGGCCAAA 
CCTCTAATGG AGTTGATTGA GAGGCTCGGG GGCTGGAACA TCACAGGTCC CTGGGCCAAG 
GACAACTTCC AGGACACCCT GCAGGTGGTC ACCGCCCACT ACCGCACCTC ACCCTTCTTC 
TCTGTCTATG TCAGTGCCGA TTCCAAGAAC TCCAACAGCA ACGTGATCCA GGTGGACCAG 
TCTGGCCTGG GCTTGCCCTC GAGAGACTAT TACCTGAACA AAACTGAAAA CGAGAAGGTG 
CTGACCGGAT ATCTGAACTA CATGGTCCAG CTGGGGAAGC TGCTGGGCGG CGGGGACGAG 
GAGGCCATCC GGCCCCAGAT GCAGCAGATC TTGGACTTTG AGACGGCACT GGCCAACATC 
ACCATCCCAC AGGAGAAGCG CCGTGATGAG GAGCTCATCT ACCACAAAGT GACGGCAGCC 
GAGCTGCAGA CCTTGGCACC CGCCATCAAC TGGTTGCCTT TTCTCAACAC CATCTTCTAC 
CCCGTGGAGA TCAATGAATC CGAGCCTATT GTGGTCTATG ACAAGGAATA CCTTGAGCAG 
ATCTCCACTC TCATCAACAC CACCGACAGA TGCCTGCTCA ACAACTACAT GATCTGGAAC 
CTGGTGCGGA AAACAAGCTC CTTCCTTGAC CAGCGCTTTC AGGACGCCGA TGAGAAGTTC 
ATGGAAGTCA TGTACGGGAC CAAGAAGACC TGTCTTCCTC GCTGGAAGTT TTGCGTGAGT 
GACACAGAAA ACAACCTGGG CTTTGCGTTG GGCCCCATGT TTGTCAAAGC AACCTTCGCC 
GAGGACAGCA AGAGCATAGC CACCGAGATC ATCCTGGAGA TTAAGAAGGC ATTTGAGGAA 
AGCCTGAGCA CCCTGAAGTG GATGGATGAG GAAACCCGAA AATCAGCCAA GGAAAAGGCC 
GATGCCATCT ACAACATGAT AGGATACCCC AACTTCATCA TGGATCCCAA GGAGCTGGAC 
AAAGTGTTTA ATGACTACAC TGCAGTTCCA GACCTCTACT TTGAAAATGC CATGCGGTTT 
TTCAACTTCT CATGGAGGGT CACTGCCGAT CAGCTCAGGA AAGCCCCCAA CAGAGATCAG 
TGGAGCATGA CCCCGCCCAT GGTGAACGCC TACTACTCGC CCACCAAGAA TGAGATTGTG 
TTTCCGGCCG GGATCCTGCA GGCACCATTC TACACACGCT CCTCACCCAA GGCCTTAAAC 
TTTGGTGGCA TAGGTGTCGT CGTGGGCCAT GAGCTGACTC ATGCTTTTGA TGATCAAGGA 
CGGGAGTATG ACAAGGACGG GAACCTCCGG CCATGGTGGA AGAACTCATC CGTGGAGGCC 
TTCAAGCGTC AGACCGAGTG CATGGTAGAG CAGTACAGCA ACTACAGCGT GAACGGGGAG 
CCGGTGAACG GGCGGCACAC CCTGGGGGAG AACATCGCCG ACAACGGGGG TCTCAAGGCG 
GCCTATCGGG CTTACCAGAA CTGGGTGAAG AAGAACGGGG CTGAGCACTC GCTCCCCACC 
CTGGGCCTCA CCAATAACCA GCTCTTCTTC CTGGGCTTTG CACAGGTCTG GTGCTCCGTC 
CGCACACCTG AGAGCTCCCA CGAAGGCCTC ATCACCGATC CCCACAGCCC CTCTCGCTTC 
CGGGTCATCG GCTCCCTCTC CAATTCCAAG GAGTTCTCAG AACACTTCCG CTGCCCACCT 
GGCTCACCCA TGAACCCGCC TCACAAGTGC GAAGTCTGGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ECE1 ( NM_001113347.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001106818.1 Homo sapiens endothelin converting enzyme 1 (ECE1), transcript variant 3, mRNA.

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CloneID	OHu16364
Clone ID Related Accession (Same CDS sequence)	NM_001113347.1 , NM_001113347.2
Accession Version	NM_001113347.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2277bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-22
Organism	Homo sapiens(human)
Product	endothelin-converting enzyme 1 isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP234532.1, D43698.1, AL031005.2, BC106022.1 and BU733112.1. Transcript Variant: This variant (3) differs in the 5' UTR and coding region, compared to variant 1, resulting in a shorter protein (isoform 3 also known as isoform a) that has a distinct N-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.99285.1, SRR1803617.91058.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCTCTCC AGGGCCTGGG CCTGCAGCGG AACCCCTTCC TCCAAGGGAA GCGGGGCCCG 
GGGCTCACGT CTTCCCCGCC CCTCCTGCCT CCTTCCCTGC AGGTGAACTT CCACAGCCCC 
CGGAGTGGCC AGAGGTGCTG GGCTGCACGG ACCCAGGTGG AGAAGCGGCT GGTGGTGTTG 
GTGGTACTTC TGGCGGCAGG ACTGGTGGCC TGCTTGGCAG CACTGGGCAT CCAGTACCAG 
ACAAGATCCC CCTCTGTGTG CCTGAGCGAA GCTTGTGTCT CAGTGACCAG CTCCATCTTG 
AGCTCCATGG ACCCCACAGT GGACCCCTGC CATGACTTCT TCAGCTACGC CTGTGGGGGC 
TGGATCAAGG CCAACCCAGT CCCTGATGGC CACTCACGCT GGGGGACCTT CAGCAACCTC 
TGGGAACACA ACCAAGCAAT CATCAAGCAC CTCCTCGAAA ACTCCACGGC CAGCGTGAGC 
GAGGCAGAGA GAAAGGCGCA AGTATACTAC CGTGCGTGCA TGAACGAGAC CAGGATCGAG 
GAGCTCAGGG CCAAACCTCT AATGGAGTTG ATTGAGAGGC TCGGGGGCTG GAACATCACA 
GGTCCCTGGG CCAAGGACAA CTTCCAGGAC ACCCTGCAGG TGGTCACCGC CCACTACCGC 
ACCTCACCCT TCTTCTCTGT CTATGTCAGT GCCGATTCCA AGAACTCCAA CAGCAACGTG 
ATCCAGGTGG ACCAGTCTGG CCTGGGCTTG CCCTCGAGAG ACTATTACCT GAACAAAACT 
GAAAACGAGA AGGTGCTGAC CGGATATCTG AACTACATGG TCCAGCTGGG GAAGCTGCTG 
GGCGGCGGGG ACGAGGAGGC CATCCGGCCC CAGATGCAGC AGATCTTGGA CTTTGAGACG 
GCACTGGCCA ACATCACCAT CCCACAGGAG AAGCGCCGTG ATGAGGAGCT CATCTACCAC 
AAAGTGACGG CAGCCGAGCT GCAGACCTTG GCACCCGCCA TCAACTGGTT GCCTTTTCTC 
AACACCATCT TCTACCCCGT GGAGATCAAT GAATCCGAGC CTATTGTGGT CTATGACAAG 
GAATACCTTG AGCAGATCTC CACTCTCATC AACACCACCG ACAGATGCCT GCTCAACAAC 
TACATGATCT GGAACCTGGT GCGGAAAACA AGCTCCTTCC TTGACCAGCG CTTTCAGGAC 
GCCGATGAGA AGTTCATGGA AGTCATGTAC GGGACCAAGA AGACCTGTCT TCCTCGCTGG 
AAGTTTTGCG TGAGTGACAC AGAAAACAAC CTGGGCTTTG CGTTGGGCCC CATGTTTGTC 
AAAGCAACCT TCGCCGAGGA CAGCAAGAGC ATAGCCACCG AGATCATCCT GGAGATTAAG 
AAGGCATTTG AGGAAAGCCT GAGCACCCTG AAGTGGATGG ATGAGGAAAC CCGAAAATCA 
GCCAAGGAAA AGGCCGATGC CATCTACAAC ATGATAGGAT ACCCCAACTT CATCATGGAT 
CCCAAGGAGC TGGACAAAGT GTTTAATGAC TACACTGCAG TTCCAGACCT CTACTTTGAA 
AATGCCATGC GGTTTTTCAA CTTCTCATGG AGGGTCACTG CCGATCAGCT CAGGAAAGCC 
CCCAACAGAG ATCAGTGGAG CATGACCCCG CCCATGGTGA ACGCCTACTA CTCGCCCACC 
AAGAATGAGA TTGTGTTTCC GGCCGGGATC CTGCAGGCAC CATTCTACAC ACGCTCCTCA 
CCCAAGGCCT TAAACTTTGG TGGCATAGGT GTCGTCGTGG GCCATGAGCT GACTCATGCT 
TTTGATGATC AAGGACGGGA GTATGACAAG GACGGGAACC TCCGGCCATG GTGGAAGAAC 
TCATCCGTGG AGGCCTTCAA GCGTCAGACC GAGTGCATGG TAGAGCAGTA CAGCAACTAC 
AGCGTGAACG GGGAGCCGGT GAACGGGCGG CACACCCTGG GGGAGAACAT CGCCGACAAC 
GGGGGTCTCA AGGCGGCCTA TCGGGCTTAC CAGAACTGGG TGAAGAAGAA CGGGGCTGAG 
CACTCGCTCC CCACCCTGGG CCTCACCAAT AACCAGCTCT TCTTCCTGGG CTTTGCACAG 
GTCTGGTGCT CCGTCCGCAC ACCTGAGAGC TCCCACGAAG GCCTCATCAC CGATCCCCAC 
AGCCCCTCTC GCTTCCGGGT CATCGGCTCC CTCTCCAATT CCAAGGAGTT CTCAGAACAC 
TTCCGCTGCC CACCTGGCTC ACCCATGAAC CCGCCTCACA AGTGCGAAGT CTGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001106818.1
CDS	257..2533
Translation	MPLQGLGLQRNPFLQGKRGPGLTSSPPLLPPSLQVNFHSPRSGQRCWAARTQVEKRLVVLVVLLAAGLVACLAALGIQYQTRSPSVCLSEACVSVTSSILSSMDPTVDPCHDFFSYACGGWIKANPVPDGHSRWGTFSNLWEHNQAIIKHLLENSTASVSEAERKAQVYYRACMNETRIEELRAKPLMELIERLGGWNITGPWAKDNFQDTLQVVTAHYRTSPFFSVYVSADSKNSNSNVIQVDQSGLGLPSRDYYLNKTENEKVLTGYLNYMVQLGKLLGGGDEEAIRPQMQQILDFETALANITIPQEKRRDEELIYHKVTAAELQTLAPAINWLPFLNTIFYPVEINESEPIVVYDKEYLEQISTLINTTDRCLLNNYMIWNLVRKTSSFLDQRFQDADEKFMEVMYGTKKTCLPRWKFCVSDTENNLGFALGPMFVKATFAEDSKSIATEIILEIKKAFEESLSTLKWMDEETRKSAKEKADAIYNMIGYPNFIMDPKELDKVFNDYTAVPDLYFENAMRFFNFSWRVTADQLRKAPNRDQWSMTPPMVNAYYSPTKNEIVFPAGILQAPFYTRSSPKALNFGGIGVVVGHELTHAFDDQGREYDKDGNLRPWWKNSSVEAFKRQTECMVEQYSNYSVNGEPVNGRHTLGENIADNGGLKAAYRAYQNWVKKNGAEHSLPTLGLTNNQLFFLGFAQVWCSVRTPESSHEGLITDPHSPSRFRVIGSLSNSKEFSEHFRCPPGSPMNPPHKCEVW

Target ORF information:

RefSeq Version	NM_001113347.1
Organism	Homo sapiens(human)
Definition	Homo sapiens endothelin converting enzyme 1 (ECE1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001113347.1

ORF Insert Sequence:

ATGCCTCTCC AGGGCCTGGG CCTGCAGCGG AACCCCTTCC TCCAAGGGAA GCGGGGCCCG 
GGGCTCACGT CTTCCCCGCC CCTCCTGCCT CCTTCCCTGC AGGTGAACTT CCACAGCCCC 
CGGAGTGGCC AGAGGTGCTG GGCTGCACGG ACCCAGGTGG AGAAGCGGCT GGTGGTGTTG 
GTGGTACTTC TGGCGGCAGG ACTGGTGGCC TGCTTGGCAG CACTGGGCAT CCAGTACCAG 
ACAAGATCCC CCTCTGTGTG CCTGAGCGAA GCTTGTGTCT CAGTGACCAG CTCCATCTTG 
AGCTCCATGG ACCCCACAGT GGACCCCTGC CATGACTTCT TCAGCTACGC CTGTGGGGGC 
TGGATCAAGG CCAACCCAGT CCCTGATGGC CACTCACGCT GGGGGACCTT CAGCAACCTC 
TGGGAACACA ACCAAGCAAT CATCAAGCAC CTCCTCGAAA ACTCCACGGC CAGCGTGAGC 
GAGGCAGAGA GAAAGGCGCA AGTATACTAC CGTGCGTGCA TGAACGAGAC CAGGATCGAG 
GAGCTCAGGG CCAAACCTCT AATGGAGTTG ATTGAGAGGC TCGGGGGCTG GAACATCACA 
GGTCCCTGGG CCAAGGACAA CTTCCAGGAC ACCCTGCAGG TGGTCACCGC CCACTACCGC 
ACCTCACCCT TCTTCTCTGT CTATGTCAGT GCCGATTCCA AGAACTCCAA CAGCAACGTG 
ATCCAGGTGG ACCAGTCTGG CCTGGGCTTG CCCTCGAGAG ACTATTACCT GAACAAAACT 
GAAAACGAGA AGGTGCTGAC CGGATATCTG AACTACATGG TCCAGCTGGG GAAGCTGCTG 
GGCGGCGGGG ACGAGGAGGC CATCCGGCCC CAGATGCAGC AGATCTTGGA CTTTGAGACG 
GCACTGGCCA ACATCACCAT CCCACAGGAG AAGCGCCGTG ATGAGGAGCT CATCTACCAC 
AAAGTGACGG CAGCCGAGCT GCAGACCTTG GCACCCGCCA TCAACTGGTT GCCTTTTCTC 
AACACCATCT TCTACCCCGT GGAGATCAAT GAATCCGAGC CTATTGTGGT CTATGACAAG 
GAATACCTTG AGCAGATCTC CACTCTCATC AACACCACCG ACAGATGCCT GCTCAACAAC 
TACATGATCT GGAACCTGGT GCGGAAAACA AGCTCCTTCC TTGACCAGCG CTTTCAGGAC 
GCCGATGAGA AGTTCATGGA AGTCATGTAC GGGACCAAGA AGACCTGTCT TCCTCGCTGG 
AAGTTTTGCG TGAGTGACAC AGAAAACAAC CTGGGCTTTG CGTTGGGCCC CATGTTTGTC 
AAAGCAACCT TCGCCGAGGA CAGCAAGAGC ATAGCCACCG AGATCATCCT GGAGATTAAG 
AAGGCATTTG AGGAAAGCCT GAGCACCCTG AAGTGGATGG ATGAGGAAAC CCGAAAATCA 
GCCAAGGAAA AGGCCGATGC CATCTACAAC ATGATAGGAT ACCCCAACTT CATCATGGAT 
CCCAAGGAGC TGGACAAAGT GTTTAATGAC TACACTGCAG TTCCAGACCT CTACTTTGAA 
AATGCCATGC GGTTTTTCAA CTTCTCATGG AGGGTCACTG CCGATCAGCT CAGGAAAGCC 
CCCAACAGAG ATCAGTGGAG CATGACCCCG CCCATGGTGA ACGCCTACTA CTCGCCCACC 
AAGAATGAGA TTGTGTTTCC GGCCGGGATC CTGCAGGCAC CATTCTACAC ACGCTCCTCA 
CCCAAGGCCT TAAACTTTGG TGGCATAGGT GTCGTCGTGG GCCATGAGCT GACTCATGCT 
TTTGATGATC AAGGACGGGA GTATGACAAG GACGGGAACC TCCGGCCATG GTGGAAGAAC 
TCATCCGTGG AGGCCTTCAA GCGTCAGACC GAGTGCATGG TAGAGCAGTA CAGCAACTAC 
AGCGTGAACG GGGAGCCGGT GAACGGGCGG CACACCCTGG GGGAGAACAT CGCCGACAAC 
GGGGGTCTCA AGGCGGCCTA TCGGGCTTAC CAGAACTGGG TGAAGAAGAA CGGGGCTGAG 
CACTCGCTCC CCACCCTGGG CCTCACCAAT AACCAGCTCT TCTTCCTGGG CTTTGCACAG 
GTCTGGTGCT CCGTCCGCAC ACCTGAGAGC TCCCACGAAG GCCTCATCAC CGATCCCCAC 
AGCCCCTCTC GCTTCCGGGT CATCGGCTCC CTCTCCAATT CCAAGGAGTT CTCAGAACAC 
TTCCGCTGCC CACCTGGCTC ACCCATGAAC CCGCCTCACA AGTGCGAAGT CTGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ECE1 ( NM_001113347.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001106818.1 Homo sapiens endothelin converting enzyme 1 (ECE1), transcript variant 3, mRNA.

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CloneID	OHu16364
Clone ID Related Accession (Same CDS sequence)	NM_001113347.1 , NM_001113347.2
Accession Version	NM_001113347.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2277bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-03
Organism	Homo sapiens(human)
Product	endothelin-converting enzyme 1 isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP234532.1, D43698.1, AL031005.2, BC106022.1 and BU733112.1. On May 31, 2019 this sequence version replaced NM_001113347.1. Transcript Variant: This variant (3) differs in the 5' UTR and coding region, compared to variant 1, resulting in a shorter protein (isoform 3 also known as isoform a) that has a distinct N-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.91058.1, SRR1803614.131200.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCTCTCC AGGGCCTGGG CCTGCAGCGG AACCCCTTCC TCCAAGGGAA GCGGGGCCCG 
GGGCTCACGT CTTCCCCGCC CCTCCTGCCT CCTTCCCTGC AGGTGAACTT CCACAGCCCC 
CGGAGTGGCC AGAGGTGCTG GGCTGCACGG ACCCAGGTGG AGAAGCGGCT GGTGGTGTTG 
GTGGTACTTC TGGCGGCAGG ACTGGTGGCC TGCTTGGCAG CACTGGGCAT CCAGTACCAG 
ACAAGATCCC CCTCTGTGTG CCTGAGCGAA GCTTGTGTCT CAGTGACCAG CTCCATCTTG 
AGCTCCATGG ACCCCACAGT GGACCCCTGC CATGACTTCT TCAGCTACGC CTGTGGGGGC 
TGGATCAAGG CCAACCCAGT CCCTGATGGC CACTCACGCT GGGGGACCTT CAGCAACCTC 
TGGGAACACA ACCAAGCAAT CATCAAGCAC CTCCTCGAAA ACTCCACGGC CAGCGTGAGC 
GAGGCAGAGA GAAAGGCGCA AGTATACTAC CGTGCGTGCA TGAACGAGAC CAGGATCGAG 
GAGCTCAGGG CCAAACCTCT AATGGAGTTG ATTGAGAGGC TCGGGGGCTG GAACATCACA 
GGTCCCTGGG CCAAGGACAA CTTCCAGGAC ACCCTGCAGG TGGTCACCGC CCACTACCGC 
ACCTCACCCT TCTTCTCTGT CTATGTCAGT GCCGATTCCA AGAACTCCAA CAGCAACGTG 
ATCCAGGTGG ACCAGTCTGG CCTGGGCTTG CCCTCGAGAG ACTATTACCT GAACAAAACT 
GAAAACGAGA AGGTGCTGAC CGGATATCTG AACTACATGG TCCAGCTGGG GAAGCTGCTG 
GGCGGCGGGG ACGAGGAGGC CATCCGGCCC CAGATGCAGC AGATCTTGGA CTTTGAGACG 
GCACTGGCCA ACATCACCAT CCCACAGGAG AAGCGCCGTG ATGAGGAGCT CATCTACCAC 
AAAGTGACGG CAGCCGAGCT GCAGACCTTG GCACCCGCCA TCAACTGGTT GCCTTTTCTC 
AACACCATCT TCTACCCCGT GGAGATCAAT GAATCCGAGC CTATTGTGGT CTATGACAAG 
GAATACCTTG AGCAGATCTC CACTCTCATC AACACCACCG ACAGATGCCT GCTCAACAAC 
TACATGATCT GGAACCTGGT GCGGAAAACA AGCTCCTTCC TTGACCAGCG CTTTCAGGAC 
GCCGATGAGA AGTTCATGGA AGTCATGTAC GGGACCAAGA AGACCTGTCT TCCTCGCTGG 
AAGTTTTGCG TGAGTGACAC AGAAAACAAC CTGGGCTTTG CGTTGGGCCC CATGTTTGTC 
AAAGCAACCT TCGCCGAGGA CAGCAAGAGC ATAGCCACCG AGATCATCCT GGAGATTAAG 
AAGGCATTTG AGGAAAGCCT GAGCACCCTG AAGTGGATGG ATGAGGAAAC CCGAAAATCA 
GCCAAGGAAA AGGCCGATGC CATCTACAAC ATGATAGGAT ACCCCAACTT CATCATGGAT 
CCCAAGGAGC TGGACAAAGT GTTTAATGAC TACACTGCAG TTCCAGACCT CTACTTTGAA 
AATGCCATGC GGTTTTTCAA CTTCTCATGG AGGGTCACTG CCGATCAGCT CAGGAAAGCC 
CCCAACAGAG ATCAGTGGAG CATGACCCCG CCCATGGTGA ACGCCTACTA CTCGCCCACC 
AAGAATGAGA TTGTGTTTCC GGCCGGGATC CTGCAGGCAC CATTCTACAC ACGCTCCTCA 
CCCAAGGCCT TAAACTTTGG TGGCATAGGT GTCGTCGTGG GCCATGAGCT GACTCATGCT 
TTTGATGATC AAGGACGGGA GTATGACAAG GACGGGAACC TCCGGCCATG GTGGAAGAAC 
TCATCCGTGG AGGCCTTCAA GCGTCAGACC GAGTGCATGG TAGAGCAGTA CAGCAACTAC 
AGCGTGAACG GGGAGCCGGT GAACGGGCGG CACACCCTGG GGGAGAACAT CGCCGACAAC 
GGGGGTCTCA AGGCGGCCTA TCGGGCTTAC CAGAACTGGG TGAAGAAGAA CGGGGCTGAG 
CACTCGCTCC CCACCCTGGG CCTCACCAAT AACCAGCTCT TCTTCCTGGG CTTTGCACAG 
GTCTGGTGCT CCGTCCGCAC ACCTGAGAGC TCCCACGAAG GCCTCATCAC CGATCCCCAC 
AGCCCCTCTC GCTTCCGGGT CATCGGCTCC CTCTCCAATT CCAAGGAGTT CTCAGAACAC 
TTCCGCTGCC CACCTGGCTC ACCCATGAAC CCGCCTCACA AGTGCGAAGT CTGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001106818.1
CDS	182..2458
Translation	MPLQGLGLQRNPFLQGKRGPGLTSSPPLLPPSLQVNFHSPRSGQRCWAARTQVEKRLVVLVVLLAAGLVACLAALGIQYQTRSPSVCLSEACVSVTSSILSSMDPTVDPCHDFFSYACGGWIKANPVPDGHSRWGTFSNLWEHNQAIIKHLLENSTASVSEAERKAQVYYRACMNETRIEELRAKPLMELIERLGGWNITGPWAKDNFQDTLQVVTAHYRTSPFFSVYVSADSKNSNSNVIQVDQSGLGLPSRDYYLNKTENEKVLTGYLNYMVQLGKLLGGGDEEAIRPQMQQILDFETALANITIPQEKRRDEELIYHKVTAAELQTLAPAINWLPFLNTIFYPVEINESEPIVVYDKEYLEQISTLINTTDRCLLNNYMIWNLVRKTSSFLDQRFQDADEKFMEVMYGTKKTCLPRWKFCVSDTENNLGFALGPMFVKATFAEDSKSIATEIILEIKKAFEESLSTLKWMDEETRKSAKEKADAIYNMIGYPNFIMDPKELDKVFNDYTAVPDLYFENAMRFFNFSWRVTADQLRKAPNRDQWSMTPPMVNAYYSPTKNEIVFPAGILQAPFYTRSSPKALNFGGIGVVVGHELTHAFDDQGREYDKDGNLRPWWKNSSVEAFKRQTECMVEQYSNYSVNGEPVNGRHTLGENIADNGGLKAAYRAYQNWVKKNGAEHSLPTLGLTNNQLFFLGFAQVWCSVRTPESSHEGLITDPHSPSRFRVIGSLSNSKEFSEHFRCPPGSPMNPPHKCEVW

Target ORF information:

RefSeq Version	NM_001113347.2
Organism	Homo sapiens(human)
Definition	Homo sapiens endothelin converting enzyme 1 (ECE1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001113347.2

ORF Insert Sequence:

ATGCCTCTCC AGGGCCTGGG CCTGCAGCGG AACCCCTTCC TCCAAGGGAA GCGGGGCCCG 
GGGCTCACGT CTTCCCCGCC CCTCCTGCCT CCTTCCCTGC AGGTGAACTT CCACAGCCCC 
CGGAGTGGCC AGAGGTGCTG GGCTGCACGG ACCCAGGTGG AGAAGCGGCT GGTGGTGTTG 
GTGGTACTTC TGGCGGCAGG ACTGGTGGCC TGCTTGGCAG CACTGGGCAT CCAGTACCAG 
ACAAGATCCC CCTCTGTGTG CCTGAGCGAA GCTTGTGTCT CAGTGACCAG CTCCATCTTG 
AGCTCCATGG ACCCCACAGT GGACCCCTGC CATGACTTCT TCAGCTACGC CTGTGGGGGC 
TGGATCAAGG CCAACCCAGT CCCTGATGGC CACTCACGCT GGGGGACCTT CAGCAACCTC 
TGGGAACACA ACCAAGCAAT CATCAAGCAC CTCCTCGAAA ACTCCACGGC CAGCGTGAGC 
GAGGCAGAGA GAAAGGCGCA AGTATACTAC CGTGCGTGCA TGAACGAGAC CAGGATCGAG 
GAGCTCAGGG CCAAACCTCT AATGGAGTTG ATTGAGAGGC TCGGGGGCTG GAACATCACA 
GGTCCCTGGG CCAAGGACAA CTTCCAGGAC ACCCTGCAGG TGGTCACCGC CCACTACCGC 
ACCTCACCCT TCTTCTCTGT CTATGTCAGT GCCGATTCCA AGAACTCCAA CAGCAACGTG 
ATCCAGGTGG ACCAGTCTGG CCTGGGCTTG CCCTCGAGAG ACTATTACCT GAACAAAACT 
GAAAACGAGA AGGTGCTGAC CGGATATCTG AACTACATGG TCCAGCTGGG GAAGCTGCTG 
GGCGGCGGGG ACGAGGAGGC CATCCGGCCC CAGATGCAGC AGATCTTGGA CTTTGAGACG 
GCACTGGCCA ACATCACCAT CCCACAGGAG AAGCGCCGTG ATGAGGAGCT CATCTACCAC 
AAAGTGACGG CAGCCGAGCT GCAGACCTTG GCACCCGCCA TCAACTGGTT GCCTTTTCTC 
AACACCATCT TCTACCCCGT GGAGATCAAT GAATCCGAGC CTATTGTGGT CTATGACAAG 
GAATACCTTG AGCAGATCTC CACTCTCATC AACACCACCG ACAGATGCCT GCTCAACAAC 
TACATGATCT GGAACCTGGT GCGGAAAACA AGCTCCTTCC TTGACCAGCG CTTTCAGGAC 
GCCGATGAGA AGTTCATGGA AGTCATGTAC GGGACCAAGA AGACCTGTCT TCCTCGCTGG 
AAGTTTTGCG TGAGTGACAC AGAAAACAAC CTGGGCTTTG CGTTGGGCCC CATGTTTGTC 
AAAGCAACCT TCGCCGAGGA CAGCAAGAGC ATAGCCACCG AGATCATCCT GGAGATTAAG 
AAGGCATTTG AGGAAAGCCT GAGCACCCTG AAGTGGATGG ATGAGGAAAC CCGAAAATCA 
GCCAAGGAAA AGGCCGATGC CATCTACAAC ATGATAGGAT ACCCCAACTT CATCATGGAT 
CCCAAGGAGC TGGACAAAGT GTTTAATGAC TACACTGCAG TTCCAGACCT CTACTTTGAA 
AATGCCATGC GGTTTTTCAA CTTCTCATGG AGGGTCACTG CCGATCAGCT CAGGAAAGCC 
CCCAACAGAG ATCAGTGGAG CATGACCCCG CCCATGGTGA ACGCCTACTA CTCGCCCACC 
AAGAATGAGA TTGTGTTTCC GGCCGGGATC CTGCAGGCAC CATTCTACAC ACGCTCCTCA 
CCCAAGGCCT TAAACTTTGG TGGCATAGGT GTCGTCGTGG GCCATGAGCT GACTCATGCT 
TTTGATGATC AAGGACGGGA GTATGACAAG GACGGGAACC TCCGGCCATG GTGGAAGAAC 
TCATCCGTGG AGGCCTTCAA GCGTCAGACC GAGTGCATGG TAGAGCAGTA CAGCAACTAC 
AGCGTGAACG GGGAGCCGGT GAACGGGCGG CACACCCTGG GGGAGAACAT CGCCGACAAC 
GGGGGTCTCA AGGCGGCCTA TCGGGCTTAC CAGAACTGGG TGAAGAAGAA CGGGGCTGAG 
CACTCGCTCC CCACCCTGGG CCTCACCAAT AACCAGCTCT TCTTCCTGGG CTTTGCACAG 
GTCTGGTGCT CCGTCCGCAC ACCTGAGAGC TCCCACGAAG GCCTCATCAC CGATCCCCAC 
AGCCCCTCTC GCTTCCGGGT CATCGGCTCC CTCTCCAATT CCAAGGAGTT CTCAGAACAC 
TTCCGCTGCC CACCTGGCTC ACCCATGAAC CCGCCTCACA AGTGCGAAGT CTGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ECE1 ( NM_001113349.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001106820.1 Homo sapiens endothelin converting enzyme 1 (ECE1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu16471
Clone ID Related Accession (Same CDS sequence)	NM_001113349.1 , NM_001113349.2
Accession Version	NM_001113349.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2304bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Homo sapiens(human)
Product	endothelin-converting enzyme 1 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP337331.1, AJ130828.1, DA445401.1, DA445355.1, CN298523.1, CN298525.1, DA436879.1, AL031005.2, BC106022.1 and BU733112.1. Transcript Variant: This variant (2) differs in the 5' UTR and coding region, compared to variant 1, resulting in a shorter protein (isoform 2 also known as isoform d) that has a distinct N-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.91873.1, SRR1803613.26854.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGGCGC TGAGGGAGTC CGTGCTGCAT TTGGCCTTGC AGATGTCGAC GTACAAGCGG 
GCCACGCTGG ACGAGGAGGA CCTGGTGGAC TCGCTCTCCG AGGGCGACGC ATACCCCAAC 
GGCCTGCAGG TGAACTTCCA CAGCCCCCGG AGTGGCCAGA GGTGCTGGGC TGCACGGACC 
CAGGTGGAGA AGCGGCTGGT GGTGTTGGTG GTACTTCTGG CGGCAGGACT GGTGGCCTGC 
TTGGCAGCAC TGGGCATCCA GTACCAGACA AGATCCCCCT CTGTGTGCCT GAGCGAAGCT 
TGTGTCTCAG TGACCAGCTC CATCTTGAGC TCCATGGACC CCACAGTGGA CCCCTGCCAT 
GACTTCTTCA GCTACGCCTG TGGGGGCTGG ATCAAGGCCA ACCCAGTCCC TGATGGCCAC 
TCACGCTGGG GGACCTTCAG CAACCTCTGG GAACACAACC AAGCAATCAT CAAGCACCTC 
CTCGAAAACT CCACGGCCAG CGTGAGCGAG GCAGAGAGAA AGGCGCAAGT ATACTACCGT 
GCGTGCATGA ACGAGACCAG GATCGAGGAG CTCAGGGCCA AACCTCTAAT GGAGTTGATT 
GAGAGGCTCG GGGGCTGGAA CATCACAGGT CCCTGGGCCA AGGACAACTT CCAGGACACC 
CTGCAGGTGG TCACCGCCCA CTACCGCACC TCACCCTTCT TCTCTGTCTA TGTCAGTGCC 
GATTCCAAGA ACTCCAACAG CAACGTGATC CAGGTGGACC AGTCTGGCCT GGGCTTGCCC 
TCGAGAGACT ATTACCTGAA CAAAACTGAA AACGAGAAGG TGCTGACCGG ATATCTGAAC 
TACATGGTCC AGCTGGGGAA GCTGCTGGGC GGCGGGGACG AGGAGGCCAT CCGGCCCCAG 
ATGCAGCAGA TCTTGGACTT TGAGACGGCA CTGGCCAACA TCACCATCCC ACAGGAGAAG 
CGCCGTGATG AGGAGCTCAT CTACCACAAA GTGACGGCAG CCGAGCTGCA GACCTTGGCA 
CCCGCCATCA ACTGGTTGCC TTTTCTCAAC ACCATCTTCT ACCCCGTGGA GATCAATGAA 
TCCGAGCCTA TTGTGGTCTA TGACAAGGAA TACCTTGAGC AGATCTCCAC TCTCATCAAC 
ACCACCGACA GATGCCTGCT CAACAACTAC ATGATCTGGA ACCTGGTGCG GAAAACAAGC 
TCCTTCCTTG ACCAGCGCTT TCAGGACGCC GATGAGAAGT TCATGGAAGT CATGTACGGG 
ACCAAGAAGA CCTGTCTTCC TCGCTGGAAG TTTTGCGTGA GTGACACAGA AAACAACCTG 
GGCTTTGCGT TGGGCCCCAT GTTTGTCAAA GCAACCTTCG CCGAGGACAG CAAGAGCATA 
GCCACCGAGA TCATCCTGGA GATTAAGAAG GCATTTGAGG AAAGCCTGAG CACCCTGAAG 
TGGATGGATG AGGAAACCCG AAAATCAGCC AAGGAAAAGG CCGATGCCAT CTACAACATG 
ATAGGATACC CCAACTTCAT CATGGATCCC AAGGAGCTGG ACAAAGTGTT TAATGACTAC 
ACTGCAGTTC CAGACCTCTA CTTTGAAAAT GCCATGCGGT TTTTCAACTT CTCATGGAGG 
GTCACTGCCG ATCAGCTCAG GAAAGCCCCC AACAGAGATC AGTGGAGCAT GACCCCGCCC 
ATGGTGAACG CCTACTACTC GCCCACCAAG AATGAGATTG TGTTTCCGGC CGGGATCCTG 
CAGGCACCAT TCTACACACG CTCCTCACCC AAGGCCTTAA ACTTTGGTGG CATAGGTGTC 
GTCGTGGGCC ATGAGCTGAC TCATGCTTTT GATGATCAAG GACGGGAGTA TGACAAGGAC 
GGGAACCTCC GGCCATGGTG GAAGAACTCA TCCGTGGAGG CCTTCAAGCG TCAGACCGAG 
TGCATGGTAG AGCAGTACAG CAACTACAGC GTGAACGGGG AGCCGGTGAA CGGGCGGCAC 
ACCCTGGGGG AGAACATCGC CGACAACGGG GGTCTCAAGG CGGCCTATCG GGCTTACCAG 
AACTGGGTGA AGAAGAACGG GGCTGAGCAC TCGCTCCCCA CCCTGGGCCT CACCAATAAC 
CAGCTCTTCT TCCTGGGCTT TGCACAGGTC TGGTGCTCCG TCCGCACACC TGAGAGCTCC 
CACGAAGGCC TCATCACCGA TCCCCACAGC CCCTCTCGCT TCCGGGTCAT CGGCTCCCTC 
TCCAATTCCA AGGAGTTCTC AGAACACTTC CGCTGCCCAC CTGGCTCACC CATGAACCCG 
CCTCACAAGT GCGAAGTCTG GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001106820.1
CDS	76..2379
Translation	MEALRESVLHLALQMSTYKRATLDEEDLVDSLSEGDAYPNGLQVNFHSPRSGQRCWAARTQVEKRLVVLVVLLAAGLVACLAALGIQYQTRSPSVCLSEACVSVTSSILSSMDPTVDPCHDFFSYACGGWIKANPVPDGHSRWGTFSNLWEHNQAIIKHLLENSTASVSEAERKAQVYYRACMNETRIEELRAKPLMELIERLGGWNITGPWAKDNFQDTLQVVTAHYRTSPFFSVYVSADSKNSNSNVIQVDQSGLGLPSRDYYLNKTENEKVLTGYLNYMVQLGKLLGGGDEEAIRPQMQQILDFETALANITIPQEKRRDEELIYHKVTAAELQTLAPAINWLPFLNTIFYPVEINESEPIVVYDKEYLEQISTLINTTDRCLLNNYMIWNLVRKTSSFLDQRFQDADEKFMEVMYGTKKTCLPRWKFCVSDTENNLGFALGPMFVKATFAEDSKSIATEIILEIKKAFEESLSTLKWMDEETRKSAKEKADAIYNMIGYPNFIMDPKELDKVFNDYTAVPDLYFENAMRFFNFSWRVTADQLRKAPNRDQWSMTPPMVNAYYSPTKNEIVFPAGILQAPFYTRSSPKALNFGGIGVVVGHELTHAFDDQGREYDKDGNLRPWWKNSSVEAFKRQTECMVEQYSNYSVNGEPVNGRHTLGENIADNGGLKAAYRAYQNWVKKNGAEHSLPTLGLTNNQLFFLGFAQVWCSVRTPESSHEGLITDPHSPSRFRVIGSLSNSKEFSEHFRCPPGSPMNPPHKCEVW

Target ORF information:

RefSeq Version	NM_001113349.1
Organism	Homo sapiens(human)
Definition	Homo sapiens endothelin converting enzyme 1 (ECE1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001113349.1

ORF Insert Sequence:

ATGGAGGCGC TGAGGGAGTC CGTGCTGCAT TTGGCCTTGC AGATGTCGAC GTACAAGCGG 
GCCACGCTGG ACGAGGAGGA CCTGGTGGAC TCGCTCTCCG AGGGCGACGC ATACCCCAAC 
GGCCTGCAGG TGAACTTCCA CAGCCCCCGG AGTGGCCAGA GGTGCTGGGC TGCACGGACC 
CAGGTGGAGA AGCGGCTGGT GGTGTTGGTG GTACTTCTGG CGGCAGGACT GGTGGCCTGC 
TTGGCAGCAC TGGGCATCCA GTACCAGACA AGATCCCCCT CTGTGTGCCT GAGCGAAGCT 
TGTGTCTCAG TGACCAGCTC CATCTTGAGC TCCATGGACC CCACAGTGGA CCCCTGCCAT 
GACTTCTTCA GCTACGCCTG TGGGGGCTGG ATCAAGGCCA ACCCAGTCCC TGATGGCCAC 
TCACGCTGGG GGACCTTCAG CAACCTCTGG GAACACAACC AAGCAATCAT CAAGCACCTC 
CTCGAAAACT CCACGGCCAG CGTGAGCGAG GCAGAGAGAA AGGCGCAAGT ATACTACCGT 
GCGTGCATGA ACGAGACCAG GATCGAGGAG CTCAGGGCCA AACCTCTAAT GGAGTTGATT 
GAGAGGCTCG GGGGCTGGAA CATCACAGGT CCCTGGGCCA AGGACAACTT CCAGGACACC 
CTGCAGGTGG TCACCGCCCA CTACCGCACC TCACCCTTCT TCTCTGTCTA TGTCAGTGCC 
GATTCCAAGA ACTCCAACAG CAACGTGATC CAGGTGGACC AGTCTGGCCT GGGCTTGCCC 
TCGAGAGACT ATTACCTGAA CAAAACTGAA AACGAGAAGG TGCTGACCGG ATATCTGAAC 
TACATGGTCC AGCTGGGGAA GCTGCTGGGC GGCGGGGACG AGGAGGCCAT CCGGCCCCAG 
ATGCAGCAGA TCTTGGACTT TGAGACGGCA CTGGCCAACA TCACCATCCC ACAGGAGAAG 
CGCCGTGATG AGGAGCTCAT CTACCACAAA GTGACGGCAG CCGAGCTGCA GACCTTGGCA 
CCCGCCATCA ACTGGTTGCC TTTTCTCAAC ACCATCTTCT ACCCCGTGGA GATCAATGAA 
TCCGAGCCTA TTGTGGTCTA TGACAAGGAA TACCTTGAGC AGATCTCCAC TCTCATCAAC 
ACCACCGACA GATGCCTGCT CAACAACTAC ATGATCTGGA ACCTGGTGCG GAAAACAAGC 
TCCTTCCTTG ACCAGCGCTT TCAGGACGCC GATGAGAAGT TCATGGAAGT CATGTACGGG 
ACCAAGAAGA CCTGTCTTCC TCGCTGGAAG TTTTGCGTGA GTGACACAGA AAACAACCTG 
GGCTTTGCGT TGGGCCCCAT GTTTGTCAAA GCAACCTTCG CCGAGGACAG CAAGAGCATA 
GCCACCGAGA TCATCCTGGA GATTAAGAAG GCATTTGAGG AAAGCCTGAG CACCCTGAAG 
TGGATGGATG AGGAAACCCG AAAATCAGCC AAGGAAAAGG CCGATGCCAT CTACAACATG 
ATAGGATACC CCAACTTCAT CATGGATCCC AAGGAGCTGG ACAAAGTGTT TAATGACTAC 
ACTGCAGTTC CAGACCTCTA CTTTGAAAAT GCCATGCGGT TTTTCAACTT CTCATGGAGG 
GTCACTGCCG ATCAGCTCAG GAAAGCCCCC AACAGAGATC AGTGGAGCAT GACCCCGCCC 
ATGGTGAACG CCTACTACTC GCCCACCAAG AATGAGATTG TGTTTCCGGC CGGGATCCTG 
CAGGCACCAT TCTACACACG CTCCTCACCC AAGGCCTTAA ACTTTGGTGG CATAGGTGTC 
GTCGTGGGCC ATGAGCTGAC TCATGCTTTT GATGATCAAG GACGGGAGTA TGACAAGGAC 
GGGAACCTCC GGCCATGGTG GAAGAACTCA TCCGTGGAGG CCTTCAAGCG TCAGACCGAG 
TGCATGGTAG AGCAGTACAG CAACTACAGC GTGAACGGGG AGCCGGTGAA CGGGCGGCAC 
ACCCTGGGGG AGAACATCGC CGACAACGGG GGTCTCAAGG CGGCCTATCG GGCTTACCAG 
AACTGGGTGA AGAAGAACGG GGCTGAGCAC TCGCTCCCCA CCCTGGGCCT CACCAATAAC 
CAGCTCTTCT TCCTGGGCTT TGCACAGGTC TGGTGCTCCG TCCGCACACC TGAGAGCTCC 
CACGAAGGCC TCATCACCGA TCCCCACAGC CCCTCTCGCT TCCGGGTCAT CGGCTCCCTC 
TCCAATTCCA AGGAGTTCTC AGAACACTTC CGCTGCCCAC CTGGCTCACC CATGAACCCG 
CCTCACAAGT GCGAAGTCTG GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ECE1 ( NM_001113349.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001106820.1 Homo sapiens endothelin converting enzyme 1 (ECE1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu16471
Clone ID Related Accession (Same CDS sequence)	NM_001113349.1 , NM_001113349.2
Accession Version	NM_001113349.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2304bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-03
Organism	Homo sapiens(human)
Product	endothelin-converting enzyme 1 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP337331.1, AJ130828.1, DA445401.1, DA445355.1, CN298523.1, CN298525.1, DA436879.1, AL031005.2, BC106022.1 and BU733112.1. On May 31, 2019 this sequence version replaced NM_001113349.1. Transcript Variant: This variant (2) differs in the 5' UTR and coding region, compared to variant 1, resulting in a shorter protein (isoform 2 also known as isoform d) that has a distinct N-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK304167.1, SRR1803617.182758.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGGCGC TGAGGGAGTC CGTGCTGCAT TTGGCCTTGC AGATGTCGAC GTACAAGCGG 
GCCACGCTGG ACGAGGAGGA CCTGGTGGAC TCGCTCTCCG AGGGCGACGC ATACCCCAAC 
GGCCTGCAGG TGAACTTCCA CAGCCCCCGG AGTGGCCAGA GGTGCTGGGC TGCACGGACC 
CAGGTGGAGA AGCGGCTGGT GGTGTTGGTG GTACTTCTGG CGGCAGGACT GGTGGCCTGC 
TTGGCAGCAC TGGGCATCCA GTACCAGACA AGATCCCCCT CTGTGTGCCT GAGCGAAGCT 
TGTGTCTCAG TGACCAGCTC CATCTTGAGC TCCATGGACC CCACAGTGGA CCCCTGCCAT 
GACTTCTTCA GCTACGCCTG TGGGGGCTGG ATCAAGGCCA ACCCAGTCCC TGATGGCCAC 
TCACGCTGGG GGACCTTCAG CAACCTCTGG GAACACAACC AAGCAATCAT CAAGCACCTC 
CTCGAAAACT CCACGGCCAG CGTGAGCGAG GCAGAGAGAA AGGCGCAAGT ATACTACCGT 
GCGTGCATGA ACGAGACCAG GATCGAGGAG CTCAGGGCCA AACCTCTAAT GGAGTTGATT 
GAGAGGCTCG GGGGCTGGAA CATCACAGGT CCCTGGGCCA AGGACAACTT CCAGGACACC 
CTGCAGGTGG TCACCGCCCA CTACCGCACC TCACCCTTCT TCTCTGTCTA TGTCAGTGCC 
GATTCCAAGA ACTCCAACAG CAACGTGATC CAGGTGGACC AGTCTGGCCT GGGCTTGCCC 
TCGAGAGACT ATTACCTGAA CAAAACTGAA AACGAGAAGG TGCTGACCGG ATATCTGAAC 
TACATGGTCC AGCTGGGGAA GCTGCTGGGC GGCGGGGACG AGGAGGCCAT CCGGCCCCAG 
ATGCAGCAGA TCTTGGACTT TGAGACGGCA CTGGCCAACA TCACCATCCC ACAGGAGAAG 
CGCCGTGATG AGGAGCTCAT CTACCACAAA GTGACGGCAG CCGAGCTGCA GACCTTGGCA 
CCCGCCATCA ACTGGTTGCC TTTTCTCAAC ACCATCTTCT ACCCCGTGGA GATCAATGAA 
TCCGAGCCTA TTGTGGTCTA TGACAAGGAA TACCTTGAGC AGATCTCCAC TCTCATCAAC 
ACCACCGACA GATGCCTGCT CAACAACTAC ATGATCTGGA ACCTGGTGCG GAAAACAAGC 
TCCTTCCTTG ACCAGCGCTT TCAGGACGCC GATGAGAAGT TCATGGAAGT CATGTACGGG 
ACCAAGAAGA CCTGTCTTCC TCGCTGGAAG TTTTGCGTGA GTGACACAGA AAACAACCTG 
GGCTTTGCGT TGGGCCCCAT GTTTGTCAAA GCAACCTTCG CCGAGGACAG CAAGAGCATA 
GCCACCGAGA TCATCCTGGA GATTAAGAAG GCATTTGAGG AAAGCCTGAG CACCCTGAAG 
TGGATGGATG AGGAAACCCG AAAATCAGCC AAGGAAAAGG CCGATGCCAT CTACAACATG 
ATAGGATACC CCAACTTCAT CATGGATCCC AAGGAGCTGG ACAAAGTGTT TAATGACTAC 
ACTGCAGTTC CAGACCTCTA CTTTGAAAAT GCCATGCGGT TTTTCAACTT CTCATGGAGG 
GTCACTGCCG ATCAGCTCAG GAAAGCCCCC AACAGAGATC AGTGGAGCAT GACCCCGCCC 
ATGGTGAACG CCTACTACTC GCCCACCAAG AATGAGATTG TGTTTCCGGC CGGGATCCTG 
CAGGCACCAT TCTACACACG CTCCTCACCC AAGGCCTTAA ACTTTGGTGG CATAGGTGTC 
GTCGTGGGCC ATGAGCTGAC TCATGCTTTT GATGATCAAG GACGGGAGTA TGACAAGGAC 
GGGAACCTCC GGCCATGGTG GAAGAACTCA TCCGTGGAGG CCTTCAAGCG TCAGACCGAG 
TGCATGGTAG AGCAGTACAG CAACTACAGC GTGAACGGGG AGCCGGTGAA CGGGCGGCAC 
ACCCTGGGGG AGAACATCGC CGACAACGGG GGTCTCAAGG CGGCCTATCG GGCTTACCAG 
AACTGGGTGA AGAAGAACGG GGCTGAGCAC TCGCTCCCCA CCCTGGGCCT CACCAATAAC 
CAGCTCTTCT TCCTGGGCTT TGCACAGGTC TGGTGCTCCG TCCGCACACC TGAGAGCTCC 
CACGAAGGCC TCATCACCGA TCCCCACAGC CCCTCTCGCT TCCGGGTCAT CGGCTCCCTC 
TCCAATTCCA AGGAGTTCTC AGAACACTTC CGCTGCCCAC CTGGCTCACC CATGAACCCG 
CCTCACAAGT GCGAAGTCTG GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001106820.1
CDS	18..2321
Translation	MEALRESVLHLALQMSTYKRATLDEEDLVDSLSEGDAYPNGLQVNFHSPRSGQRCWAARTQVEKRLVVLVVLLAAGLVACLAALGIQYQTRSPSVCLSEACVSVTSSILSSMDPTVDPCHDFFSYACGGWIKANPVPDGHSRWGTFSNLWEHNQAIIKHLLENSTASVSEAERKAQVYYRACMNETRIEELRAKPLMELIERLGGWNITGPWAKDNFQDTLQVVTAHYRTSPFFSVYVSADSKNSNSNVIQVDQSGLGLPSRDYYLNKTENEKVLTGYLNYMVQLGKLLGGGDEEAIRPQMQQILDFETALANITIPQEKRRDEELIYHKVTAAELQTLAPAINWLPFLNTIFYPVEINESEPIVVYDKEYLEQISTLINTTDRCLLNNYMIWNLVRKTSSFLDQRFQDADEKFMEVMYGTKKTCLPRWKFCVSDTENNLGFALGPMFVKATFAEDSKSIATEIILEIKKAFEESLSTLKWMDEETRKSAKEKADAIYNMIGYPNFIMDPKELDKVFNDYTAVPDLYFENAMRFFNFSWRVTADQLRKAPNRDQWSMTPPMVNAYYSPTKNEIVFPAGILQAPFYTRSSPKALNFGGIGVVVGHELTHAFDDQGREYDKDGNLRPWWKNSSVEAFKRQTECMVEQYSNYSVNGEPVNGRHTLGENIADNGGLKAAYRAYQNWVKKNGAEHSLPTLGLTNNQLFFLGFAQVWCSVRTPESSHEGLITDPHSPSRFRVIGSLSNSKEFSEHFRCPPGSPMNPPHKCEVW

Target ORF information:

RefSeq Version	NM_001113349.2
Organism	Homo sapiens(human)
Definition	Homo sapiens endothelin converting enzyme 1 (ECE1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001113349.2

ORF Insert Sequence:

ATGGAGGCGC TGAGGGAGTC CGTGCTGCAT TTGGCCTTGC AGATGTCGAC GTACAAGCGG 
GCCACGCTGG ACGAGGAGGA CCTGGTGGAC TCGCTCTCCG AGGGCGACGC ATACCCCAAC 
GGCCTGCAGG TGAACTTCCA CAGCCCCCGG AGTGGCCAGA GGTGCTGGGC TGCACGGACC 
CAGGTGGAGA AGCGGCTGGT GGTGTTGGTG GTACTTCTGG CGGCAGGACT GGTGGCCTGC 
TTGGCAGCAC TGGGCATCCA GTACCAGACA AGATCCCCCT CTGTGTGCCT GAGCGAAGCT 
TGTGTCTCAG TGACCAGCTC CATCTTGAGC TCCATGGACC CCACAGTGGA CCCCTGCCAT 
GACTTCTTCA GCTACGCCTG TGGGGGCTGG ATCAAGGCCA ACCCAGTCCC TGATGGCCAC 
TCACGCTGGG GGACCTTCAG CAACCTCTGG GAACACAACC AAGCAATCAT CAAGCACCTC 
CTCGAAAACT CCACGGCCAG CGTGAGCGAG GCAGAGAGAA AGGCGCAAGT ATACTACCGT 
GCGTGCATGA ACGAGACCAG GATCGAGGAG CTCAGGGCCA AACCTCTAAT GGAGTTGATT 
GAGAGGCTCG GGGGCTGGAA CATCACAGGT CCCTGGGCCA AGGACAACTT CCAGGACACC 
CTGCAGGTGG TCACCGCCCA CTACCGCACC TCACCCTTCT TCTCTGTCTA TGTCAGTGCC 
GATTCCAAGA ACTCCAACAG CAACGTGATC CAGGTGGACC AGTCTGGCCT GGGCTTGCCC 
TCGAGAGACT ATTACCTGAA CAAAACTGAA AACGAGAAGG TGCTGACCGG ATATCTGAAC 
TACATGGTCC AGCTGGGGAA GCTGCTGGGC GGCGGGGACG AGGAGGCCAT CCGGCCCCAG 
ATGCAGCAGA TCTTGGACTT TGAGACGGCA CTGGCCAACA TCACCATCCC ACAGGAGAAG 
CGCCGTGATG AGGAGCTCAT CTACCACAAA GTGACGGCAG CCGAGCTGCA GACCTTGGCA 
CCCGCCATCA ACTGGTTGCC TTTTCTCAAC ACCATCTTCT ACCCCGTGGA GATCAATGAA 
TCCGAGCCTA TTGTGGTCTA TGACAAGGAA TACCTTGAGC AGATCTCCAC TCTCATCAAC 
ACCACCGACA GATGCCTGCT CAACAACTAC ATGATCTGGA ACCTGGTGCG GAAAACAAGC 
TCCTTCCTTG ACCAGCGCTT TCAGGACGCC GATGAGAAGT TCATGGAAGT CATGTACGGG 
ACCAAGAAGA CCTGTCTTCC TCGCTGGAAG TTTTGCGTGA GTGACACAGA AAACAACCTG 
GGCTTTGCGT TGGGCCCCAT GTTTGTCAAA GCAACCTTCG CCGAGGACAG CAAGAGCATA 
GCCACCGAGA TCATCCTGGA GATTAAGAAG GCATTTGAGG AAAGCCTGAG CACCCTGAAG 
TGGATGGATG AGGAAACCCG AAAATCAGCC AAGGAAAAGG CCGATGCCAT CTACAACATG 
ATAGGATACC CCAACTTCAT CATGGATCCC AAGGAGCTGG ACAAAGTGTT TAATGACTAC 
ACTGCAGTTC CAGACCTCTA CTTTGAAAAT GCCATGCGGT TTTTCAACTT CTCATGGAGG 
GTCACTGCCG ATCAGCTCAG GAAAGCCCCC AACAGAGATC AGTGGAGCAT GACCCCGCCC 
ATGGTGAACG CCTACTACTC GCCCACCAAG AATGAGATTG TGTTTCCGGC CGGGATCCTG 
CAGGCACCAT TCTACACACG CTCCTCACCC AAGGCCTTAA ACTTTGGTGG CATAGGTGTC 
GTCGTGGGCC ATGAGCTGAC TCATGCTTTT GATGATCAAG GACGGGAGTA TGACAAGGAC 
GGGAACCTCC GGCCATGGTG GAAGAACTCA TCCGTGGAGG CCTTCAAGCG TCAGACCGAG 
TGCATGGTAG AGCAGTACAG CAACTACAGC GTGAACGGGG AGCCGGTGAA CGGGCGGCAC 
ACCCTGGGGG AGAACATCGC CGACAACGGG GGTCTCAAGG CGGCCTATCG GGCTTACCAG 
AACTGGGTGA AGAAGAACGG GGCTGAGCAC TCGCTCCCCA CCCTGGGCCT CACCAATAAC 
CAGCTCTTCT TCCTGGGCTT TGCACAGGTC TGGTGCTCCG TCCGCACACC TGAGAGCTCC 
CACGAAGGCC TCATCACCGA TCCCCACAGC CCCTCTCGCT TCCGGGTCAT CGGCTCCCTC 
TCCAATTCCA AGGAGTTCTC AGAACACTTC CGCTGCCCAC CTGGCTCACC CATGAACCCG 
CCTCACAAGT GCGAAGTCTG GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ECE1 ( XM_011540872.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011539174.1 Homo sapiens endothelin converting enzyme 1 (ECE1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu57423
Clone ID Related Accession (Same CDS sequence)	XM_011540872.2
Accession Version	XM_011540872.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2337bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	endothelin-converting enzyme 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011540872.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGCATGA GAATCATCAG GAATGCTGGT TACAAATGCA AGTTTCATTG CCCTCATCCT 
AGAGGTTCTA ACTCAATGTC GACGTACAAG CGGGCCACGC TGGACGAGGA GGACCTGGTG 
GACTCGCTCT CCGAGGGCGA CGCATACCCC AACGGCCTGC AGGTGAACTT CCACAGCCCC 
CGGAGTGGCC AGAGGTGCTG GGCTGCACGG ACCCAGGTGG AGAAGCGGCT GGTGGTGTTG 
GTGGTACTTC TGGCGGCAGG ACTGGTGGCC TGCTTGGCAG CACTGGGCAT CCAGTACCAG 
ACAAGATCCC CCTCTGTGTG CCTGAGCGAA GCTTGTGTCT CAGTGACCAG CTCCATCTTG 
AGCTCCATGG ACCCCACAGT GGACCCCTGC CATGACTTCT TCAGCTACGC CTGTGGGGGC 
TGGATCAAGG CCAACCCAGT CCCTGATGGC CACTCACGCT GGGGGACCTT CAGCAACCTC 
TGGGAACACA ACCAAGCAAT CATCAAGCAC CTCCTCGAAA ACTCCACGGC CAGCGTGAGC 
GAGGCAGAGA GAAAGGCGCA AGTATACTAC CGTGCGTGCA TGAACGAGAC CAGGATCGAG 
GAGCTCAGGG CCAAACCTCT AATGGAGTTG ATTGAGAGGC TCGGGGGCTG GAACATCACA 
GGTCCCTGGG CCAAGGACAA CTTCCAGGAC ACCCTGCAGG TGGTCACCGC CCACTACCGC 
ACCTCACCCT TCTTCTCTGT CTATGTCAGT GCCGATTCCA AGAACTCCAA CAGCAACGTG 
ATCCAGGTGG ACCAGTCTGG CCTGGGCTTG CCCTCGAGAG ACTATTACCT GAACAAAACT 
GAAAACGAGA AGGTGCTGAC CGGATATCTG AACTACATGG TCCAGCTGGG GAAGCTGCTG 
GGCGGCGGGG ACGAGGAGGC CATCCGGCCC CAGATGCAGC AGATCTTGGA CTTTGAGACG 
GCACTGGCCA ACATCACCAT CCCACAGGAG AAGCGCCGTG ATGAGGAGCT CATCTACCAC 
AAAGTGACGG CAGCCGAGCT GCAGACCTTG GCACCCGCCA TCAACTGGTT GCCTTTTCTC 
AACACCATCT TCTACCCCGT GGAGATCAAT GAATCCGAGC CTATTGTGGT CTATGACAAG 
GAATACCTTG AGCAGATCTC CACTCTCATC AACACCACCG ACAGATGCCT GCTCAACAAC 
TACATGATCT GGAACCTGGT GCGGAAAACA AGCTCCTTCC TTGACCAGCG CTTTCAGGAC 
GCCGATGAGA AGTTCATGGA AGTCATGTAC GGGACCAAGA AGACCTGTCT TCCTCGCTGG 
AAGTTTTGCG TGAGTGACAC AGAAAACAAC CTGGGCTTTG CGTTGGGCCC CATGTTTGTC 
AAAGCAACCT TCGCCGAGGA CAGCAAGAGC ATAGCCACCG AGATCATCCT GGAGATTAAG 
AAGGCATTTG AGGAAAGCCT GAGCACCCTG AAGTGGATGG ATGAGGAAAC CCGAAAATCA 
GCCAAGGAAA AGGCCGATGC CATCTACAAC ATGATAGGAT ACCCCAACTT CATCATGGAT 
CCCAAGGAGC TGGACAAAGT GTTTAATGAC TACACTGCAG TTCCAGACCT CTACTTTGAA 
AATGCCATGC GGTTTTTCAA CTTCTCATGG AGGGTCACTG CCGATCAGCT CAGGAAAGCC 
CCCAACAGAG ATCAGTGGAG CATGACCCCG CCCATGGTGA ACGCCTACTA CTCGCCCACC 
AAGAATGAGA TTGTGTTTCC GGCCGGGATC CTGCAGGCAC CATTCTACAC ACGCTCCTCA 
CCCAAGGCCT TAAACTTTGG TGGCATAGGT GTCGTCGTGG GCCATGAGCT GACTCATGCT 
TTTGATGATC AAGGACGGGA GTATGACAAG GACGGGAACC TCCGGCCATG GTGGAAGAAC 
TCATCCGTGG AGGCCTTCAA GCGTCAGACC GAGTGCATGG TAGAGCAGTA CAGCAACTAC 
AGCGTGAACG GGGAGCCGGT GAACGGGCGG CACACCCTGG GGGAGAACAT CGCCGACAAC 
GGGGGTCTCA AGGCGGCCTA TCGGGCTTAC CAGAACTGGG TGAAGAAGAA CGGGGCTGAG 
CACTCGCTCC CCACCCTGGG CCTCACCAAT AACCAGCTCT TCTTCCTGGG CTTTGCACAG 
GTCTGGTGCT CCGTCCGCAC ACCTGAGAGC TCCCACGAAG GCCTCATCAC CGATCCCCAC 
AGCCCCTCTC GCTTCCGGGT CATCGGCTCC CTCTCCAATT CCAAGGAGTT CTCAGAACAC 
TTCCGCTGCC CACCTGGCTC ACCCATGAAC CCGCCTCACA AGTGCGAAGT CTGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011539174.1
CDS	804..3140
Translation	MCMRIIRNAGYKCKFHCPHPRGSNSMSTYKRATLDEEDLVDSLSEGDAYPNGLQVNFHSPRSGQRCWAARTQVEKRLVVLVVLLAAGLVACLAALGIQYQTRSPSVCLSEACVSVTSSILSSMDPTVDPCHDFFSYACGGWIKANPVPDGHSRWGTFSNLWEHNQAIIKHLLENSTASVSEAERKAQVYYRACMNETRIEELRAKPLMELIERLGGWNITGPWAKDNFQDTLQVVTAHYRTSPFFSVYVSADSKNSNSNVIQVDQSGLGLPSRDYYLNKTENEKVLTGYLNYMVQLGKLLGGGDEEAIRPQMQQILDFETALANITIPQEKRRDEELIYHKVTAAELQTLAPAINWLPFLNTIFYPVEINESEPIVVYDKEYLEQISTLINTTDRCLLNNYMIWNLVRKTSSFLDQRFQDADEKFMEVMYGTKKTCLPRWKFCVSDTENNLGFALGPMFVKATFAEDSKSIATEIILEIKKAFEESLSTLKWMDEETRKSAKEKADAIYNMIGYPNFIMDPKELDKVFNDYTAVPDLYFENAMRFFNFSWRVTADQLRKAPNRDQWSMTPPMVNAYYSPTKNEIVFPAGILQAPFYTRSSPKALNFGGIGVVVGHELTHAFDDQGREYDKDGNLRPWWKNSSVEAFKRQTECMVEQYSNYSVNGEPVNGRHTLGENIADNGGLKAAYRAYQNWVKKNGAEHSLPTLGLTNNQLFFLGFAQVWCSVRTPESSHEGLITDPHSPSRFRVIGSLSNSKEFSEHFRCPPGSPMNPPHKCEVW

Target ORF information:

RefSeq Version	XM_011540872.2
Organism	Homo sapiens(human)
Definition	Homo sapiens endothelin converting enzyme 1 (ECE1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011540872.2

ORF Insert Sequence:

ATGTGCATGA GAATCATCAG GAATGCTGGT TACAAATGCA AGTTTCATTG CCCTCATCCT 
AGAGGTTCTA ACTCAATGTC GACGTACAAG CGGGCCACGC TGGACGAGGA GGACCTGGTG 
GACTCGCTCT CCGAGGGCGA CGCATACCCC AACGGCCTGC AGGTGAACTT CCACAGCCCC 
CGGAGTGGCC AGAGGTGCTG GGCTGCACGG ACCCAGGTGG AGAAGCGGCT GGTGGTGTTG 
GTGGTACTTC TGGCGGCAGG ACTGGTGGCC TGCTTGGCAG CACTGGGCAT CCAGTACCAG 
ACAAGATCCC CCTCTGTGTG CCTGAGCGAA GCTTGTGTCT CAGTGACCAG CTCCATCTTG 
AGCTCCATGG ACCCCACAGT GGACCCCTGC CATGACTTCT TCAGCTACGC CTGTGGGGGC 
TGGATCAAGG CCAACCCAGT CCCTGATGGC CACTCACGCT GGGGGACCTT CAGCAACCTC 
TGGGAACACA ACCAAGCAAT CATCAAGCAC CTCCTCGAAA ACTCCACGGC CAGCGTGAGC 
GAGGCAGAGA GAAAGGCGCA AGTATACTAC CGTGCGTGCA TGAACGAGAC CAGGATCGAG 
GAGCTCAGGG CCAAACCTCT AATGGAGTTG ATTGAGAGGC TCGGGGGCTG GAACATCACA 
GGTCCCTGGG CCAAGGACAA CTTCCAGGAC ACCCTGCAGG TGGTCACCGC CCACTACCGC 
ACCTCACCCT TCTTCTCTGT CTATGTCAGT GCCGATTCCA AGAACTCCAA CAGCAACGTG 
ATCCAGGTGG ACCAGTCTGG CCTGGGCTTG CCCTCGAGAG ACTATTACCT GAACAAAACT 
GAAAACGAGA AGGTGCTGAC CGGATATCTG AACTACATGG TCCAGCTGGG GAAGCTGCTG 
GGCGGCGGGG ACGAGGAGGC CATCCGGCCC CAGATGCAGC AGATCTTGGA CTTTGAGACG 
GCACTGGCCA ACATCACCAT CCCACAGGAG AAGCGCCGTG ATGAGGAGCT CATCTACCAC 
AAAGTGACGG CAGCCGAGCT GCAGACCTTG GCACCCGCCA TCAACTGGTT GCCTTTTCTC 
AACACCATCT TCTACCCCGT GGAGATCAAT GAATCCGAGC CTATTGTGGT CTATGACAAG 
GAATACCTTG AGCAGATCTC CACTCTCATC AACACCACCG ACAGATGCCT GCTCAACAAC 
TACATGATCT GGAACCTGGT GCGGAAAACA AGCTCCTTCC TTGACCAGCG CTTTCAGGAC 
GCCGATGAGA AGTTCATGGA AGTCATGTAC GGGACCAAGA AGACCTGTCT TCCTCGCTGG 
AAGTTTTGCG TGAGTGACAC AGAAAACAAC CTGGGCTTTG CGTTGGGCCC CATGTTTGTC 
AAAGCAACCT TCGCCGAGGA CAGCAAGAGC ATAGCCACCG AGATCATCCT GGAGATTAAG 
AAGGCATTTG AGGAAAGCCT GAGCACCCTG AAGTGGATGG ATGAGGAAAC CCGAAAATCA 
GCCAAGGAAA AGGCCGATGC CATCTACAAC ATGATAGGAT ACCCCAACTT CATCATGGAT 
CCCAAGGAGC TGGACAAAGT GTTTAATGAC TACACTGCAG TTCCAGACCT CTACTTTGAA 
AATGCCATGC GGTTTTTCAA CTTCTCATGG AGGGTCACTG CCGATCAGCT CAGGAAAGCC 
CCCAACAGAG ATCAGTGGAG CATGACCCCG CCCATGGTGA ACGCCTACTA CTCGCCCACC 
AAGAATGAGA TTGTGTTTCC GGCCGGGATC CTGCAGGCAC CATTCTACAC ACGCTCCTCA 
CCCAAGGCCT TAAACTTTGG TGGCATAGGT GTCGTCGTGG GCCATGAGCT GACTCATGCT 
TTTGATGATC AAGGACGGGA GTATGACAAG GACGGGAACC TCCGGCCATG GTGGAAGAAC 
TCATCCGTGG AGGCCTTCAA GCGTCAGACC GAGTGCATGG TAGAGCAGTA CAGCAACTAC 
AGCGTGAACG GGGAGCCGGT GAACGGGCGG CACACCCTGG GGGAGAACAT CGCCGACAAC 
GGGGGTCTCA AGGCGGCCTA TCGGGCTTAC CAGAACTGGG TGAAGAAGAA CGGGGCTGAG 
CACTCGCTCC CCACCCTGGG CCTCACCAAT AACCAGCTCT TCTTCCTGGG CTTTGCACAG 
GTCTGGTGCT CCGTCCGCAC ACCTGAGAGC TCCCACGAAG GCCTCATCAC CGATCCCCAC 
AGCCCCTCTC GCTTCCGGGT CATCGGCTCC CTCTCCAATT CCAAGGAGTT CTCAGAACAC 
TTCCGCTGCC CACCTGGCTC ACCCATGAAC CCGCCTCACA AGTGCGAAGT CTGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	ECE1
Entrez Gene ID	1889
Full Name	endothelin converting enzyme 1
Synonyms	ECE
General protein information	Preferred Names endothelin converting enzyme 1 Names endothelin-converting enzyme 1 ECE-1
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 1 Map Location: 1p36.12
Summary	The protein encoded by this gene is involved in proteolytic processing of endothelin precursors to biologically active peptides. Mutations in this gene are associated with Hirschsprung disease, cardiac defects and autonomic dysfunction. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene.[provided by RefSeq, Sep 2009].
Disorder MIM:	600423
Disorder Html:	?Hirschsprung disease, cardiac defects, and autonomic dysfunction, 613870 (3); {Hypertension, essential, susceptibility to}, 145500 (3)

Pan troglodytes (chimpanzee)	ECE1	XP_001162780.1
Mus musculus (house mouse)	Ece1	NP_955011.1
Bos taurus (cattle)	ECE1	NP_851352.1
Rattus norvegicus (Norway rat)	Ece1	NP_446048.2
Danio rerio (zebrafish)	ece1	NP_001071260.1
	ECE1	XP_001099089.1
Gallus gallus (chicken)	ECE1	NP_990048.1
Canis lupus familiaris (dog)	ECE1	XP_544514.3
Drosophila melanogaster (fruit fly)	Nep3	NP_001245776.1
Homo sapiens (human)	ECE1	NP_001106820.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007796	XP_317711.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

ECE1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

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