DUSP3 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	DUSP3
Entrez Gene ID	1845
Full Name	dual specificity phosphatase 3
Synonyms	VHR
General protein information	Preferred Names dual specificity phosphatase 3 Names dual specificity protein phosphatase 3 dual specificity protein phosphatase VHR serine/threonine specific protein phosphatase vaccinia H1-related phosphatase vaccinia virus phosphatase VH1-related
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 17 Map Location: 17q21.31
Summary	The protein encoded by this gene is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein (MAP) kinase superfamily (MAPK/ERK, SAPK/JNK, p38), which are associated with cellular proliferation and differentiation. Different members of the family of dual specificity phosphatases show distinct substrate specificities for various MAP kinases, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. This gene maps in a region that contains the BRCA1 locus which confers susceptibility to breast and ovarian cancer. Although DUSP3 is expressed in both breast and ovarian tissues, mutation screening in breast cancer pedigrees and in sporadic tumors was negative, leading to the conclusion that this gene is not BRCA1. [provided by RefSeq, Jul 2008].
Disorder MIM:	600183

mRNA and Protein(s)

mRNA	Protein	Name
NM_004090.4	NP_004081.1	dual specificity protein phosphatase 3
NM_004090.3	NP_004081.1	dual specificity protein phosphatase 3

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	DUSP3	XP_003315464.1
Canis lupus familiaris (dog)	DUSP3	XP_537624.3
Xenopus tropicalis (tropical clawed frog)	LOC100498529	XP_002937631.1
Homo sapiens (human)	DUSP3	NP_004081.1
	DUSP3	XP_001113754.1
Bos taurus (cattle)	DUSP3	NP_001069842.1
Mus musculus (house mouse)	Dusp3	NP_082483.1
Rattus norvegicus (Norway rat)	Dusp3	NP_001166847.1
Gallus gallus (chicken)	DUSP3	XP_423280.4
Danio rerio (zebrafish)	dusp3a	XP_001340818.2

Gene Ontology
Bibliography

Related articles in PubMed

Deficiency in VHR/DUSP3, a suppressor of focal adhesion kinase, reveals its role in regulating cell adhesion and migration.
Chen YR, Chou HC, Yang CH, Chen HY, Liu YW, Lin TY, Yeh CL, Chao WT, Tsou HH, Chuang HC, Tan TH
Oncogene36(47)6509-6517(2017 Nov)

Loss of DUSP3 activity radiosensitizes human tumor cell lines via attenuation of DNA repair pathways.
Torres TEP, Russo LC, Santos A, Marques GR, Magalhaes YT, Tabassum S, Forti FL
Biochimica et biophysica acta1861(7)1879-1894(2017 07)

Leveraging Reciprocity to Identify and Characterize Unknown Allosteric Sites in Protein Tyrosine Phosphatases.
Cui DS, Beaumont V, Ginther PS, Lipchock JM, Loria JP
Journal of molecular biology429(15)2360-2372(2017 Jul)

VRK3-mediated nuclear localization of HSP70 prevents glutamate excitotoxicity-induced apoptosis and A? accumulation via enhancement of ERK phosphatase VHR activity.
Song H, Kim W, Kim SH, Kim KT
Scientific reports638452(2016 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data indicated that regulating non-receptor tyrosine kinase FAK and cell migration is a newly revealed function of VHR/DUSP3.
Title: Deficiency in VHR/DUSP3, a suppressor of focal adhesion kinase, reveals its role in regulating cell adhesion and migration.

The loss of DUSP3 activity markedly increases gamma radiation-induced DNA strand breaks, suggesting a potential novel role for DUSP3 in DNA repair.
Title: Loss of DUSP3 activity radiosensitizes human tumor cell lines via attenuation of DNA repair pathways.

In PTP1B and VHR, two new allosteric clusters were identified in each enzyme.
Title: Leveraging Reciprocity to Identify and Characterize Unknown Allosteric Sites in Protein Tyrosine Phosphatases.

In the phosphatase-silenced cells, the normal bipolar spindle structure was restored by chemical inhibition of Erk1/2 and ectopic overexpression of Dusp3. We propose that at M phase Dusp3 keeps Erk1/2 activity in check to facilitate normal mitosis.
Title: Reduced levels of Dusp3/Vhr phosphatase impair normal spindle bipolarity in an Erk1/2 activity-dependent manner.

Our results demonstrate that DUSP3 plays a key and nonredundant role as a regulator of innate immune responses
Title: DUSP3 Genetic Deletion Confers M2-like Macrophage-Dependent Tolerance to Septic Shock.

The following DUSP3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DUSP3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu23379	NM_004090.4 Latest version!	Homo sapiens dual specificity phosphatase 3 (DUSP3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
View Old Accession Versions >>
OHu23379	NM_004090.3	Homo sapiens dual specificity phosphatase 3 (DUSP3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DUSP3 ( NM_004090.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_004081.1 Homo sapiens dual specificity phosphatase 3 (DUSP3), mRNA.

Price & Availability↑

CloneID	OHu23379
Clone ID Related Accession (Same CDS sequence)	NM_004090.4 , NM_004090.3
Accession Version	NM_004090.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 558bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-25
Organism	Homo sapiens(human)
Product	dual specificity protein phosphatase 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA104250.1, BC002682.1, AK129822.1, AC055813.9, DB171573.1, AK055834.1, DA167802.1, DA075379.1, BQ652984.1 and BQ575811.1. On Nov 22, 2018 this sequence version replaced NM_004090.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.105724.1, SRR1803611.73793.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000226004.8/ ENSP00000226004.2 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGTCGGGCT CGTTCGAGCT CTCGGTGCAG GATCTCAACG ACCTGCTCTC GGACGGCAGC 
GGCTGCTACA GCCTCCCGAG CCAGCCCTGC AACGAGGTCA CCCCGCGGAT CTACGTGGGC 
AACGCGTCTG TGGCTCAGGA CATCCCCAAG CTGCAGAAAC TAGGCATCAC CCATGTGCTG 
AACGCGGCTG AGGGCAGGTC CTTCATGCAC GTCAACACCA ATGCCAACTT CTACAAGGAC 
TCCGGCATCA CATACCTGGG CATCAAGGCC AACGACACAC AGGAGTTCAA CCTCAGCGCT 
TACTTTGAAA GGGCTGCCGA CTTCATTGAC CAGGCTTTGG CTCAAAAGAA TGGCCGGGTG 
CTCGTCCACT GCCGGGAAGG TTATAGCCGC TCCCCAACGC TAGTTATCGC CTACCTCATG 
ATGCGGCAGA AGATGGACGT CAAGTCTGCC CTGAGCATCG TGAGGCAGAA CCGTGAGATC 
GGCCCCAACG ATGGCTTCCT GGCCCAGCTC TGCCAGCTCA ATGACAGACT AGCCAAGGAG 
GGGAAGTTGA AACCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004081.1
CDS	54..611
Translation	MSGSFELSVQDLNDLLSDGSGCYSLPSQPCNEVTPRIYVGNASVAQDIPKLQKLGITHVLNAAEGRSFMHVNTNANFYKDSGITYLGIKANDTQEFNLSAYFERAADFIDQALAQKNGRVLVHCREGYSRSPTLVIAYLMMRQKMDVKSALSIVRQNREIGPNDGFLAQLCQLNDRLAKEGKLKP

Target ORF information:

RefSeq Version	NM_004090.4
Organism	Homo sapiens(human)
Definition	Homo sapiens dual specificity phosphatase 3 (DUSP3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004090.4

ORF Insert Sequence:

ATGTCGGGCT CGTTCGAGCT CTCGGTGCAG GATCTCAACG ACCTGCTCTC GGACGGCAGC 
GGCTGCTACA GCCTCCCGAG CCAGCCCTGC AACGAGGTCA CCCCGCGGAT CTACGTGGGC 
AACGCGTCTG TGGCTCAGGA CATCCCCAAG CTGCAGAAAC TAGGCATCAC CCATGTGCTG 
AACGCGGCTG AGGGCAGGTC CTTCATGCAC GTCAACACCA ATGCCAACTT CTACAAGGAC 
TCCGGCATCA CATACCTGGG CATCAAGGCC AACGACACAC AGGAGTTCAA CCTCAGCGCT 
TACTTTGAAA GGGCTGCCGA CTTCATTGAC CAGGCTTTGG CTCAAAAGAA TGGCCGGGTG 
CTCGTCCACT GCCGGGAAGG TTATAGCCGC TCCCCAACGC TAGTTATCGC CTACCTCATG 
ATGCGGCAGA AGATGGACGT CAAGTCTGCC CTGAGCATCG TGAGGCAGAA CCGTGAGATC 
GGCCCCAACG ATGGCTTCCT GGCCCAGCTC TGCCAGCTCA ATGACAGACT AGCCAAGGAG 
GGGAAGTTGA AACCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DUSP3 ( NM_004090.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_004081.1 Homo sapiens dual specificity phosphatase 3 (DUSP3), mRNA.

Price & Availability↑

CloneID	OHu23379
Clone ID Related Accession (Same CDS sequence)	NM_004090.4 , NM_004090.3
Accession Version	NM_004090.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 558bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-10
Organism	Homo sapiens(human)
Product	dual specificity protein phosphatase 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA104250.1, BC002682.1, AK129822.1, AC055813.9, DB171573.1, AK055834.1, DA167802.1, DA075379.1, BQ652984.1 and BQ575811.1. On Feb 8, 2008 this sequence version replaced NM_004090.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.145822.1, SRR1803616.97564.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCGGGCT CGTTCGAGCT CTCGGTGCAG GATCTCAACG ACCTGCTCTC GGACGGCAGC 
GGCTGCTACA GCCTCCCGAG CCAGCCCTGC AACGAGGTCA CCCCGCGGAT CTACGTGGGC 
AACGCGTCTG TGGCTCAGGA CATCCCCAAG CTGCAGAAAC TAGGCATCAC CCATGTGCTG 
AACGCGGCTG AGGGCAGGTC CTTCATGCAC GTCAACACCA ATGCCAACTT CTACAAGGAC 
TCCGGCATCA CATACCTGGG CATCAAGGCC AACGACACAC AGGAGTTCAA CCTCAGCGCT 
TACTTTGAAA GGGCTGCCGA CTTCATTGAC CAGGCTTTGG CTCAAAAGAA TGGCCGGGTG 
CTCGTCCACT GCCGGGAAGG TTATAGCCGC TCCCCAACGC TAGTTATCGC CTACCTCATG 
ATGCGGCAGA AGATGGACGT CAAGTCTGCC CTGAGCATCG TGAGGCAGAA CCGTGAGATC 
GGCCCCAACG ATGGCTTCCT GGCCCAGCTC TGCCAGCTCA ATGACAGACT AGCCAAGGAG 
GGGAAGTTGA AACCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004081.1
CDS	77..634
Translation	MSGSFELSVQDLNDLLSDGSGCYSLPSQPCNEVTPRIYVGNASVAQDIPKLQKLGITHVLNAAEGRSFMHVNTNANFYKDSGITYLGIKANDTQEFNLSAYFERAADFIDQALAQKNGRVLVHCREGYSRSPTLVIAYLMMRQKMDVKSALSIVRQNREIGPNDGFLAQLCQLNDRLAKEGKLKP

Target ORF information:

RefSeq Version	NM_004090.3
Organism	Homo sapiens(human)
Definition	Homo sapiens dual specificity phosphatase 3 (DUSP3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004090.3

ORF Insert Sequence:

ATGTCGGGCT CGTTCGAGCT CTCGGTGCAG GATCTCAACG ACCTGCTCTC GGACGGCAGC 
GGCTGCTACA GCCTCCCGAG CCAGCCCTGC AACGAGGTCA CCCCGCGGAT CTACGTGGGC 
AACGCGTCTG TGGCTCAGGA CATCCCCAAG CTGCAGAAAC TAGGCATCAC CCATGTGCTG 
AACGCGGCTG AGGGCAGGTC CTTCATGCAC GTCAACACCA ATGCCAACTT CTACAAGGAC 
TCCGGCATCA CATACCTGGG CATCAAGGCC AACGACACAC AGGAGTTCAA CCTCAGCGCT 
TACTTTGAAA GGGCTGCCGA CTTCATTGAC CAGGCTTTGG CTCAAAAGAA TGGCCGGGTG 
CTCGTCCACT GCCGGGAAGG TTATAGCCGC TCCCCAACGC TAGTTATCGC CTACCTCATG 
ATGCGGCAGA AGATGGACGT CAAGTCTGCC CTGAGCATCG TGAGGCAGAA CCGTGAGATC 
GGCCCCAACG ATGGCTTCCT GGCCCAGCTC TGCCAGCTCA ATGACAGACT AGCCAAGGAG 
GGGAAGTTGA AACCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Deficiency in VHR/DUSP3, a suppressor of focal adhesion kinase, reveals its role in regulating cell adhesion and migration.
Oncogene36(47)6509-6517(2017 Nov)
Chen YR,Chou HC,Yang CH,Chen HY,Liu YW,Lin TY,Yeh CL,Chao WT,Tsou HH,Chuang HC,Tan TH

Loss of DUSP3 activity radiosensitizes human tumor cell lines via attenuation of DNA repair pathways.
Biochimica et biophysica acta1861(7)1879-1894(2017 07)
Torres TEP,Russo LC,Santos A,Marques GR,Magalhaes YT,Tabassum S,Forti FL

Leveraging Reciprocity to Identify and Characterize Unknown Allosteric Sites in Protein Tyrosine Phosphatases.
Journal of molecular biology429(15)2360-2372(2017 Jul)
Cui DS,Beaumont V,Ginther PS,Lipchock JM,Loria JP

VRK3-mediated nuclear localization of HSP70 prevents glutamate excitotoxicity-induced apoptosis and A? accumulation via enhancement of ERK phosphatase VHR activity.
Scientific reports638452(2016 Dec)
Song H,Kim W,Kim SH,Kim KT

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DUSP3 cDNA ORF clone, Homo sapiens(human)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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