AGT cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	AGT
Entrez Gene ID	183
Full Name	angiotensinogen
Synonyms	ANHU,SERPINA8,hFLT1
General protein information	Preferred Names angiotensinogen Names angiotensinogen alpha-1 antiproteinase, antitrypsin angiotensin I angiotensin II fetal-liver predominant transporter 1 pre-angiotensinogen serine (or cysteine) proteinase inhibitor serpin A8 serpin peptidase inhibitor, clade A, member 8
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 1 Map Location: 1q42.2
Summary	The protein encoded by this gene, pre-angiotensinogen or angiotensinogen precursor, is expressed in the liver and is cleaved by the enzyme renin in response to lowered blood pressure. The resulting product, angiotensin I, is then cleaved by angiotensin converting enzyme (ACE) to generate the physiologically active enzyme angiotensin II. The protein is involved in maintaining blood pressure and in the pathogenesis of essential hypertension and preeclampsia. Mutations in this gene are associated with susceptibility to essential hypertension, and can cause renal tubular dysgenesis, a severe disorder of renal tubular development. Defects in this gene have also been associated with non-familial structural atrial fibrillation, and inflammatory bowel disease. [provided by RefSeq, Jul 2008].
Disorder MIM:	106150
Disorder Html:	{Hypertension, essential, susceptibility to}, 145500 (3); {Preeclampsia, susceptibility to} (3); Renal tubular dysgenesis, 267430 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_000029.4	NP_000020.1	angiotensinogen preproprotein
NM_000029.3	NP_000020.1	angiotensinogen preproprotein

Pathways from BioSystems

Homologies

Homo sapiens (human)	AGT	NP_000020.1
Canis lupus familiaris (dog)	AGT	XP_005618890.1
Mus musculus (house mouse)	Agt	NP_031454.3
	AGT	XP_001107374.2
Danio rerio (zebrafish)	agt	NP_932329.1
Xenopus tropicalis (tropical clawed frog)	agt	XP_002931552.2
Pan troglodytes (chimpanzee)	AGT	NP_001009032.1
Gallus gallus (chicken)	AGT	XP_004935548.1
Rattus norvegicus (Norway rat)	Agt	NP_602308.1

Gene Ontology

The following AGT gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AGT cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu26781	NM_000029.4 Latest version!	Homo sapiens angiotensinogen (AGT), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
View Old Accession Versions >>
OHu26781	NM_000029.3	Homo sapiens angiotensinogen (AGT), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

AGT ( NM_000029.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_000020.1 Homo sapiens angiotensinogen (AGT), mRNA.

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CloneID	OHu26781
Clone ID Related Accession (Same CDS sequence)	NM_000029.4 , NM_000029.3
Accession Version	NM_000029.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1458bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-22
Organism	Homo sapiens(human)
Product	angiotensinogen preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK312291.1, K02215.1, T97863.1 and AW128887.1. This sequence is a reference standard in the RefSeqGene project. On Nov 20, 2018 this sequence version replaced NM_000029.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.5887.1, SRR3476690.183350.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000366667.5/ ENSP00000355627.4 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGCGGAAGC GAGCACCCCA GTCTGAGATG GCTCCTGCCG GTGTGAGCCT GAGGGCCACC 
ATCCTCTGCC TCCTGGCCTG GGCTGGCCTG GCTGCAGGTG ACCGGGTGTA CATACACCCC 
TTCCACCTCG TCATCCACAA TGAGAGTACC TGTGAGCAGC TGGCAAAGGC CAATGCCGGG 
AAGCCCAAAG ACCCCACCTT CATACCTGCT CCAATTCAGG CCAAGACATC CCCTGTGGAT 
GAAAAGGCCC TACAGGACCA GCTGGTGCTA GTCGCTGCAA AACTTGACAC CGAAGACAAG 
TTGAGGGCCG CAATGGTCGG GATGCTGGCC AACTTCTTGG GCTTCCGTAT ATATGGCATG 
CACAGTGAGC TATGGGGCGT GGTCCATGGG GCCACCGTCC TCTCCCCAAC GGCTGTCTTT 
GGCACCCTGG CCTCTCTCTA TCTGGGAGCC TTGGACCACA CAGCTGACAG GCTACAGGCA 
ATCCTGGGTG TTCCTTGGAA GGACAAGAAC TGCACCTCCC GGCTGGATGC GCACAAGGTC 
CTGTCTGCCC TGCAGGCTGT ACAGGGCCTG CTAGTGGCCC AGGGCAGGGC TGATAGCCAG 
GCCCAGCTGC TGCTGTCCAC GGTGGTGGGC GTGTTCACAG CCCCAGGCCT GCACCTGAAG 
CAGCCGTTTG TGCAGGGCCT GGCTCTCTAT ACCCCTGTGG TCCTCCCACG CTCTCTGGAC 
TTCACAGAAC TGGATGTTGC TGCTGAGAAG ATTGACAGGT TCATGCAGGC TGTGACAGGA 
TGGAAGACTG GCTGCTCCCT GATGGGAGCC AGTGTGGACA GCACCCTGGC TTTCAACACC 
TACGTCCACT TCCAAGGGAA GATGAAGGGC TTCTCCCTGC TGGCCGAGCC CCAGGAGTTC 
TGGGTGGACA ACAGCACCTC AGTGTCTGTT CCCATGCTCT CTGGCATGGG CACCTTCCAG 
CACTGGAGTG ACATCCAGGA CAACTTCTCG GTGACTCAAG TGCCCTTCAC TGAGAGCGCC 
TGCCTGCTGC TGATCCAGCC TCACTATGCC TCTGACCTGG ACAAGGTGGA GGGTCTCACT 
TTCCAGCAAA ACTCCCTCAA CTGGATGAAG AAACTATCTC CCCGGACCAT CCACCTGACC 
ATGCCCCAAC TGGTGCTGCA AGGATCTTAT GACCTGCAGG ACCTGCTCGC CCAGGCTGAG 
CTGCCCGCCA TTCTGCACAC CGAGCTGAAC CTGCAAAAAT TGAGCAATGA CCGCATCAGG 
GTGGGGGAGG TGCTGAACAG CATTTTTTTT GAGCTTGAAG CGGATGAGAG AGAGCCCACA 
GAGTCTACCC AACAGCTTAA CAAGCCTGAG GTCTTGGAGG TGACCCTGAA CCGCCCATTC 
CTGTTTGCTG TGTATGATCA AAGCGCCACT GCCCTGCACT TCCTGGGCCG CGTGGCCAAC 
CCGCTGAGCA CAGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000020.1
CDS	41..1498
Translation	MRKRAPQSEMAPAGVSLRATILCLLAWAGLAAGDRVYIHPFHLVIHNESTCEQLAKANAGKPKDPTFIPAPIQAKTSPVDEKALQDQLVLVAAKLDTEDKLRAAMVGMLANFLGFRIYGMHSELWGVVHGATVLSPTAVFGTLASLYLGALDHTADRLQAILGVPWKDKNCTSRLDAHKVLSALQAVQGLLVAQGRADSQAQLLLSTVVGVFTAPGLHLKQPFVQGLALYTPVVLPRSLDFTELDVAAEKIDRFMQAVTGWKTGCSLMGASVDSTLAFNTYVHFQGKMKGFSLLAEPQEFWVDNSTSVSVPMLSGMGTFQHWSDIQDNFSVTQVPFTESACLLLIQPHYASDLDKVEGLTFQQNSLNWMKKLSPRTIHLTMPQLVLQGSYDLQDLLAQAELPAILHTELNLQKLSNDRIRVGEVLNSIFFELEADEREPTESTQQLNKPEVLEVTLNRPFLFAVYDQSATALHFLGRVANPLSTA

Target ORF information:

RefSeq Version	NM_000029.4
Organism	Homo sapiens(human)
Definition	Homo sapiens angiotensinogen (AGT), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000029.4

ORF Insert Sequence:

ATGCGGAAGC GAGCACCCCA GTCTGAGATG GCTCCTGCCG GTGTGAGCCT GAGGGCCACC 
ATCCTCTGCC TCCTGGCCTG GGCTGGCCTG GCTGCAGGTG ACCGGGTGTA CATACACCCC 
TTCCACCTCG TCATCCACAA TGAGAGTACC TGTGAGCAGC TGGCAAAGGC CAATGCCGGG 
AAGCCCAAAG ACCCCACCTT CATACCTGCT CCAATTCAGG CCAAGACATC CCCTGTGGAT 
GAAAAGGCCC TACAGGACCA GCTGGTGCTA GTCGCTGCAA AACTTGACAC CGAAGACAAG 
TTGAGGGCCG CAATGGTCGG GATGCTGGCC AACTTCTTGG GCTTCCGTAT ATATGGCATG 
CACAGTGAGC TATGGGGCGT GGTCCATGGG GCCACCGTCC TCTCCCCAAC GGCTGTCTTT 
GGCACCCTGG CCTCTCTCTA TCTGGGAGCC TTGGACCACA CAGCTGACAG GCTACAGGCA 
ATCCTGGGTG TTCCTTGGAA GGACAAGAAC TGCACCTCCC GGCTGGATGC GCACAAGGTC 
CTGTCTGCCC TGCAGGCTGT ACAGGGCCTG CTAGTGGCCC AGGGCAGGGC TGATAGCCAG 
GCCCAGCTGC TGCTGTCCAC GGTGGTGGGC GTGTTCACAG CCCCAGGCCT GCACCTGAAG 
CAGCCGTTTG TGCAGGGCCT GGCTCTCTAT ACCCCTGTGG TCCTCCCACG CTCTCTGGAC 
TTCACAGAAC TGGATGTTGC TGCTGAGAAG ATTGACAGGT TCATGCAGGC TGTGACAGGA 
TGGAAGACTG GCTGCTCCCT GATGGGAGCC AGTGTGGACA GCACCCTGGC TTTCAACACC 
TACGTCCACT TCCAAGGGAA GATGAAGGGC TTCTCCCTGC TGGCCGAGCC CCAGGAGTTC 
TGGGTGGACA ACAGCACCTC AGTGTCTGTT CCCATGCTCT CTGGCATGGG CACCTTCCAG 
CACTGGAGTG ACATCCAGGA CAACTTCTCG GTGACTCAAG TGCCCTTCAC TGAGAGCGCC 
TGCCTGCTGC TGATCCAGCC TCACTATGCC TCTGACCTGG ACAAGGTGGA GGGTCTCACT 
TTCCAGCAAA ACTCCCTCAA CTGGATGAAG AAACTATCTC CCCGGACCAT CCACCTGACC 
ATGCCCCAAC TGGTGCTGCA AGGATCTTAT GACCTGCAGG ACCTGCTCGC CCAGGCTGAG 
CTGCCCGCCA TTCTGCACAC CGAGCTGAAC CTGCAAAAAT TGAGCAATGA CCGCATCAGG 
GTGGGGGAGG TGCTGAACAG CATTTTTTTT GAGCTTGAAG CGGATGAGAG AGAGCCCACA 
GAGTCTACCC AACAGCTTAA CAAGCCTGAG GTCTTGGAGG TGACCCTGAA CCGCCCATTC 
CTGTTTGCTG TGTATGATCA AAGCGCCACT GCCCTGCACT TCCTGGGCCG CGTGGCCAAC 
CCGCTGAGCA CAGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AGT ( NM_000029.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_000020.1 Homo sapiens angiotensinogen (AGT), mRNA.

Price & Availability↑

CloneID	OHu26781
Clone ID Related Accession (Same CDS sequence)	NM_000029.4 , NM_000029.3
Accession Version	NM_000029.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1458bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-05
Organism	Homo sapiens(human)
Product	angiotensinogen preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC412932.1, AK312291.1, K02215.1, T97863.1 and AW128887.1. This sequence is a reference standard in the RefSeqGene project. On May 21, 2008 this sequence version replaced NM_000029.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.129758.1, SRR1660807.230853.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCGGAAGC GAGCACCCCA GTCTGAGATG GCTCCTGCCG GTGTGAGCCT GAGGGCCACC 
ATCCTCTGCC TCCTGGCCTG GGCTGGCCTG GCTGCAGGTG ACCGGGTGTA CATACACCCC 
TTCCACCTCG TCATCCACAA TGAGAGTACC TGTGAGCAGC TGGCAAAGGC CAATGCCGGG 
AAGCCCAAAG ACCCCACCTT CATACCTGCT CCAATTCAGG CCAAGACATC CCCTGTGGAT 
GAAAAGGCCC TACAGGACCA GCTGGTGCTA GTCGCTGCAA AACTTGACAC CGAAGACAAG 
TTGAGGGCCG CAATGGTCGG GATGCTGGCC AACTTCTTGG GCTTCCGTAT ATATGGCATG 
CACAGTGAGC TATGGGGCGT GGTCCATGGG GCCACCGTCC TCTCCCCAAC GGCTGTCTTT 
GGCACCCTGG CCTCTCTCTA TCTGGGAGCC TTGGACCACA CAGCTGACAG GCTACAGGCA 
ATCCTGGGTG TTCCTTGGAA GGACAAGAAC TGCACCTCCC GGCTGGATGC GCACAAGGTC 
CTGTCTGCCC TGCAGGCTGT ACAGGGCCTG CTAGTGGCCC AGGGCAGGGC TGATAGCCAG 
GCCCAGCTGC TGCTGTCCAC GGTGGTGGGC GTGTTCACAG CCCCAGGCCT GCACCTGAAG 
CAGCCGTTTG TGCAGGGCCT GGCTCTCTAT ACCCCTGTGG TCCTCCCACG CTCTCTGGAC 
TTCACAGAAC TGGATGTTGC TGCTGAGAAG ATTGACAGGT TCATGCAGGC TGTGACAGGA 
TGGAAGACTG GCTGCTCCCT GATGGGAGCC AGTGTGGACA GCACCCTGGC TTTCAACACC 
TACGTCCACT TCCAAGGGAA GATGAAGGGC TTCTCCCTGC TGGCCGAGCC CCAGGAGTTC 
TGGGTGGACA ACAGCACCTC AGTGTCTGTT CCCATGCTCT CTGGCATGGG CACCTTCCAG 
CACTGGAGTG ACATCCAGGA CAACTTCTCG GTGACTCAAG TGCCCTTCAC TGAGAGCGCC 
TGCCTGCTGC TGATCCAGCC TCACTATGCC TCTGACCTGG ACAAGGTGGA GGGTCTCACT 
TTCCAGCAAA ACTCCCTCAA CTGGATGAAG AAACTATCTC CCCGGACCAT CCACCTGACC 
ATGCCCCAAC TGGTGCTGCA AGGATCTTAT GACCTGCAGG ACCTGCTCGC CCAGGCTGAG 
CTGCCCGCCA TTCTGCACAC CGAGCTGAAC CTGCAAAAAT TGAGCAATGA CCGCATCAGG 
GTGGGGGAGG TGCTGAACAG CATTTTTTTT GAGCTTGAAG CGGATGAGAG AGAGCCCACA 
GAGTCTACCC AACAGCTTAA CAAGCCTGAG GTCTTGGAGG TGACCCTGAA CCGCCCATTC 
CTGTTTGCTG TGTATGATCA AAGCGCCACT GCCCTGCACT TCCTGGGCCG CGTGGCCAAC 
CCGCTGAGCA CAGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000020.1
CDS	509..1966
Translation	MRKRAPQSEMAPAGVSLRATILCLLAWAGLAAGDRVYIHPFHLVIHNESTCEQLAKANAGKPKDPTFIPAPIQAKTSPVDEKALQDQLVLVAAKLDTEDKLRAAMVGMLANFLGFRIYGMHSELWGVVHGATVLSPTAVFGTLASLYLGALDHTADRLQAILGVPWKDKNCTSRLDAHKVLSALQAVQGLLVAQGRADSQAQLLLSTVVGVFTAPGLHLKQPFVQGLALYTPVVLPRSLDFTELDVAAEKIDRFMQAVTGWKTGCSLMGASVDSTLAFNTYVHFQGKMKGFSLLAEPQEFWVDNSTSVSVPMLSGMGTFQHWSDIQDNFSVTQVPFTESACLLLIQPHYASDLDKVEGLTFQQNSLNWMKKLSPRTIHLTMPQLVLQGSYDLQDLLAQAELPAILHTELNLQKLSNDRIRVGEVLNSIFFELEADEREPTESTQQLNKPEVLEVTLNRPFLFAVYDQSATALHFLGRVANPLSTA

Target ORF information:

RefSeq Version	NM_000029.3
Organism	Homo sapiens(human)
Definition	Homo sapiens angiotensinogen (AGT), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000029.3

ORF Insert Sequence:

ATGCGGAAGC GAGCACCCCA GTCTGAGATG GCTCCTGCCG GTGTGAGCCT GAGGGCCACC 
ATCCTCTGCC TCCTGGCCTG GGCTGGCCTG GCTGCAGGTG ACCGGGTGTA CATACACCCC 
TTCCACCTCG TCATCCACAA TGAGAGTACC TGTGAGCAGC TGGCAAAGGC CAATGCCGGG 
AAGCCCAAAG ACCCCACCTT CATACCTGCT CCAATTCAGG CCAAGACATC CCCTGTGGAT 
GAAAAGGCCC TACAGGACCA GCTGGTGCTA GTCGCTGCAA AACTTGACAC CGAAGACAAG 
TTGAGGGCCG CAATGGTCGG GATGCTGGCC AACTTCTTGG GCTTCCGTAT ATATGGCATG 
CACAGTGAGC TATGGGGCGT GGTCCATGGG GCCACCGTCC TCTCCCCAAC GGCTGTCTTT 
GGCACCCTGG CCTCTCTCTA TCTGGGAGCC TTGGACCACA CAGCTGACAG GCTACAGGCA 
ATCCTGGGTG TTCCTTGGAA GGACAAGAAC TGCACCTCCC GGCTGGATGC GCACAAGGTC 
CTGTCTGCCC TGCAGGCTGT ACAGGGCCTG CTAGTGGCCC AGGGCAGGGC TGATAGCCAG 
GCCCAGCTGC TGCTGTCCAC GGTGGTGGGC GTGTTCACAG CCCCAGGCCT GCACCTGAAG 
CAGCCGTTTG TGCAGGGCCT GGCTCTCTAT ACCCCTGTGG TCCTCCCACG CTCTCTGGAC 
TTCACAGAAC TGGATGTTGC TGCTGAGAAG ATTGACAGGT TCATGCAGGC TGTGACAGGA 
TGGAAGACTG GCTGCTCCCT GATGGGAGCC AGTGTGGACA GCACCCTGGC TTTCAACACC 
TACGTCCACT TCCAAGGGAA GATGAAGGGC TTCTCCCTGC TGGCCGAGCC CCAGGAGTTC 
TGGGTGGACA ACAGCACCTC AGTGTCTGTT CCCATGCTCT CTGGCATGGG CACCTTCCAG 
CACTGGAGTG ACATCCAGGA CAACTTCTCG GTGACTCAAG TGCCCTTCAC TGAGAGCGCC 
TGCCTGCTGC TGATCCAGCC TCACTATGCC TCTGACCTGG ACAAGGTGGA GGGTCTCACT 
TTCCAGCAAA ACTCCCTCAA CTGGATGAAG AAACTATCTC CCCGGACCAT CCACCTGACC 
ATGCCCCAAC TGGTGCTGCA AGGATCTTAT GACCTGCAGG ACCTGCTCGC CCAGGCTGAG 
CTGCCCGCCA TTCTGCACAC CGAGCTGAAC CTGCAAAAAT TGAGCAATGA CCGCATCAGG 
GTGGGGGAGG TGCTGAACAG CATTTTTTTT GAGCTTGAAG CGGATGAGAG AGAGCCCACA 
GAGTCTACCC AACAGCTTAA CAAGCCTGAG GTCTTGGAGG TGACCCTGAA CCGCCCATTC 
CTGTTTGCTG TGTATGATCA AAGCGCCACT GCCCTGCACT TCCTGGGCCG CGTGGCCAAC 
CCGCTGAGCA CAGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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AGT cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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