JAG1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	JAG1
Entrez Gene ID	182
Full Name	jagged 1
Synonyms	AGS,AGS1,AHD,AWS,CD339,HJ1,JAGL1
General protein information	Preferred Names jagged 1 Names protein jagged-1
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 20 Map Location: 20p12.2
Summary	The jagged 1 protein encoded by JAG1 is the human homolog of the Drosophilia jagged protein. Human jagged 1 is the ligand for the receptor notch 1, the latter a human homolog of the Drosophilia jagged receptor notch. Mutations that alter the jagged 1 protein cause Alagille syndrome. Jagged 1 signalling through notch 1 has also been shown to play a role in hematopoiesis. [provided by RefSeq, Jul 2008].
Disorder MIM:	601920
Disorder Html:	Alagille syndrome 1, 118450 (3); Tetralogy of Fallot, 187500 (3); ?Deafness, congenital heart defects, and posterior embryotoxon (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_000214.3	NP_000205.1	protein jagged-1 precursor
NM_000214.2	NP_000205.1	protein jagged-1 precursor
XM_017027707.1	XP_016883196.1	protein jagged-1 isoform X1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	JAG1	XP_005634840.1
Bos taurus (cattle)	JAG1	NP_001178107.1
Rattus norvegicus (Norway rat)	Jag1	NP_062020.1
Gallus gallus (chicken)	JAG1	XP_415035.4
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004154	XP_313038.5
Pan troglodytes (chimpanzee)	JAG1	XP_001169666.1
Drosophila melanogaster (fruit fly)	Ser	NP_524527.3
	JAG1	XP_001116216.2
Mus musculus (house mouse)	Jag1	NP_038850.1
Xenopus tropicalis (tropical clawed frog)	jag1	XP_002931784.1
Homo sapiens (human)	JAG1	NP_000205.1
Danio rerio (zebrafish)	jag1b	NP_571938.1

Gene Ontology

The following JAG1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the JAG1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu23253	NM_000214.3 Latest version!	Homo sapiens jagged canonical Notch ligand 1 (JAG1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$459.50-$643.30 $919.00
OHu92943	XM_017027707.1 Latest version!	Homo sapiens jagged 1 (JAG1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	15-17	$559.30 $799.00
View Old Accession Versions >>
OHu23253	NM_000214.2	Homo sapiens jagged 1 (JAG1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$459.50-$643.30 $919.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

JAG1 ( NM_000214.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_000205.1 Homo sapiens jagged canonical Notch ligand 1 (JAG1), mRNA.

Price & Availability↑

CloneID	OHu23253
Clone ID Related Accession (Same CDS sequence)	NM_000214.3 , NM_000214.2
Accession Version	NM_000214.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3657bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Homo sapiens(human)
Product	protein jagged-1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BF056748.1, U73936.1, AF028593.1, AA933912.1 and AI268246.1. This sequence is a reference standard in the RefSeqGene project. On May 8, 2018 this sequence version replaced NM_000214.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF003837.1, BC098393.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000254958.10/ ENSP00000254958.4 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGCGTTCCC CACGGACGCG CGGCCGGTCC GGGCGCCCCC TAAGCCTCCT GCTCGCCCTG 
CTCTGTGCCC TGCGAGCCAA GGTGTGTGGG GCCTCGGGTC AGTTCGAGTT GGAGATCCTG 
TCCATGCAGA ACGTGAACGG GGAGCTGCAG AACGGGAACT GCTGCGGCGG CGCCCGGAAC 
CCGGGAGACC GCAAGTGCAC CCGCGACGAG TGTGACACAT ACTTCAAAGT GTGCCTCAAG 
GAGTATCAGT CCCGCGTCAC GGCCGGGGGG CCCTGCAGCT TCGGCTCAGG GTCCACGCCT 
GTCATCGGGG GCAACACCTT CAACCTCAAG GCCAGCCGCG GCAACGACCG CAACCGCATC 
GTGCTGCCTT TCAGTTTCGC CTGGCCGAGG TCCTATACGT TGCTTGTGGA GGCGTGGGAT 
TCCAGTAATG ACACCGTTCA ACCTGACAGT ATTATTGAAA AGGCTTCTCA CTCGGGCATG 
ATCAACCCCA GCCGGCAGTG GCAGACGCTG AAGCAGAACA CGGGCGTTGC CCACTTTGAG 
TATCAGATCC GCGTGACCTG TGATGACTAC TACTATGGCT TTGGCTGCAA TAAGTTCTGC 
CGCCCCAGAG ATGACTTCTT TGGACACTAT GCCTGTGACC AGAATGGCAA CAAAACTTGC 
ATGGAAGGCT GGATGGGCCC CGAATGTAAC AGAGCTATTT GCCGACAAGG CTGCAGTCCT 
AAGCATGGGT CTTGCAAACT CCCAGGTGAC TGCAGGTGCC AGTACGGCTG GCAAGGCCTG 
TACTGTGATA AGTGCATCCC ACACCCGGGA TGCGTCCACG GCATCTGTAA TGAGCCCTGG 
CAGTGCCTCT GTGAGACCAA CTGGGGCGGC CAGCTCTGTG ACAAAGATCT CAATTACTGT 
GGGACTCATC AGCCGTGTCT CAACGGGGGA ACTTGTAGCA ACACAGGCCC TGACAAATAT 
CAGTGTTCCT GCCCTGAGGG GTATTCAGGA CCCAACTGTG AAATTGCTGA GCACGCCTGC 
CTCTCTGATC CCTGTCACAA CAGAGGCAGC TGTAAGGAGA CCTCCCTGGG CTTTGAGTGT 
GAGTGTTCCC CAGGCTGGAC CGGCCCCACA TGCTCTACAA ACATTGATGA CTGTTCTCCT 
AATAACTGTT CCCACGGGGG CACCTGCCAG GACCTGGTTA ACGGATTTAA GTGTGTGTGC 
CCCCCACAGT GGACTGGGAA AACGTGCCAG TTAGATGCAA ATGAATGTGA GGCCAAACCT 
TGTGTAAACG CCAAATCCTG TAAGAATCTC ATTGCCAGCT ACTACTGCGA CTGTCTTCCC 
GGCTGGATGG GTCAGAATTG TGACATAAAT ATTAATGACT GCCTTGGCCA GTGTCAGAAT 
GACGCCTCCT GTCGGGATTT GGTTAATGGT TATCGCTGTA TCTGTCCACC TGGCTATGCA 
GGCGATCACT GTGAGAGAGA CATCGATGAA TGTGCCAGCA ACCCCTGTTT GAATGGGGGT 
CACTGTCAGA ATGAAATCAA CAGATTCCAG TGTCTGTGTC CCACTGGTTT CTCTGGAAAC 
CTCTGTCAGC TGGACATCGA TTATTGTGAG CCTAATCCCT GCCAGAACGG TGCCCAGTGC 
TACAACCGTG CCAGTGACTA TTTCTGCAAG TGCCCCGAGG ACTATGAGGG CAAGAACTGC 
TCACACCTGA AAGACCACTG CCGCACGACC CCCTGTGAAG TGATTGACAG CTGCACAGTG 
GCCATGGCTT CCAACGACAC ACCTGAAGGG GTGCGGTATA TTTCCTCCAA CGTCTGTGGT 
CCTCACGGGA AGTGCAAGAG TCAGTCGGGA GGCAAATTCA CCTGTGACTG TAACAAAGGC 
TTCACGGGAA CATACTGCCA TGAAAATATT AATGACTGTG AGAGCAACCC TTGTAGAAAC 
GGTGGCACTT GCATCGATGG TGTCAACTCC TACAAGTGCA TCTGTAGTGA CGGCTGGGAG 
GGGGCCTACT GTGAAACCAA TATTAATGAC TGCAGCCAGA ACCCCTGCCA CAATGGGGGC 
ACGTGTCGCG ACCTGGTCAA TGACTTCTAC TGTGACTGTA AAAATGGGTG GAAAGGAAAG 
ACCTGCCACT CACGTGACAG TCAGTGTGAT GAGGCCACGT GCAACAACGG TGGCACCTGC 
TATGATGAGG GGGATGCTTT TAAGTGCATG TGTCCTGGCG GCTGGGAAGG AACAACCTGT 
AACATAGCCC GAAACAGTAG CTGCCTGCCC AACCCCTGCC ATAATGGGGG CACATGTGTG 
GTCAACGGCG AGTCCTTTAC GTGCGTCTGC AAGGAAGGCT GGGAGGGGCC CATCTGTGCT 
CAGAATACCA ATGACTGCAG CCCTCATCCC TGTTACAACA GCGGCACCTG TGTGGATGGA 
GACAACTGGT ACCGGTGCGA ATGTGCCCCG GGTTTTGCTG GGCCCGACTG CAGAATAAAC 
ATCAATGAAT GCCAGTCTTC ACCTTGTGCC TTTGGAGCGA CCTGTGTGGA TGAGATCAAT 
GGCTACCGGT GTGTCTGCCC TCCAGGGCAC AGTGGTGCCA AGTGCCAGGA AGTTTCAGGG 
AGACCTTGCA TCACCATGGG GAGTGTGATA CCAGATGGGG CCAAATGGGA TGATGACTGT 
AATACCTGCC AGTGCCTGAA TGGACGGATC GCCTGCTCAA AGGTCTGGTG TGGCCCTCGA 
CCTTGCCTGC TCCACAAAGG GCACAGCGAG TGCCCCAGCG GGCAGAGCTG CATCCCCATC 
CTGGACGACC AGTGCTTCGT CCACCCCTGC ACTGGTGTGG GCGAGTGTCG GTCTTCCAGT 
CTCCAGCCGG TGAAGACAAA GTGCACCTCT GACTCCTATT ACCAGGATAA CTGTGCGAAC 
ATCACATTTA CCTTTAACAA GGAGATGATG TCACCAGGTC TTACTACGGA GCACATTTGC 
AGTGAATTGA GGAATTTGAA TATTTTGAAG AATGTTTCCG CTGAATATTC AATCTACATC 
GCTTGCGAGC CTTCCCCTTC AGCGAACAAT GAAATACATG TGGCCATTTC TGCTGAAGAT 
ATACGGGATG ATGGGAACCC GATCAAGGAA ATCACTGACA AAATAATCGA TCTTGTTAGT 
AAACGTGATG GAAACAGCTC GCTGATTGCT GCCGTTGCAG AAGTAAGAGT TCAGAGGCGG 
CCTCTGAAGA ACAGAACAGA TTTCCTTGTT CCCTTGCTGA GCTCTGTCTT AACTGTGGCT 
TGGATCTGTT GCTTGGTGAC GGCCTTCTAC TGGTGCCTGC GGAAGCGGCG GAAGCCGGGC 
AGCCACACAC ACTCAGCCTC TGAGGACAAC ACCACCAACA ACGTGCGGGA GCAGCTGAAC 
CAGATCAAAA ACCCCATTGA GAAACATGGG GCCAACACGG TCCCCATCAA GGATTATGAG 
AACAAGAACT CCAAAATGTC TAAAATAAGG ACACACAATT CTGAAGTAGA AGAGGACGAC 
ATGGACAAAC ACCAGCAGAA AGCCCGGTTT GCCAAGCAGC CGGCGTACAC GCTGGTAGAC 
AGAGAAGAGA AGCCCCCCAA CGGCACGCCG ACAAAACACC CAAACTGGAC AAACAAACAG 
GACAACAGAG ACTTGGAAAG TGCCCAGAGC TTAAACCGAA TGGAGTACAT CGTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000205.1
CDS	470..4126
Translation	MRSPRTRGRSGRPLSLLLALLCALRAKVCGASGQFELEILSMQNVNGELQNGNCCGGARNPGDRKCTRDECDTYFKVCLKEYQSRVTAGGPCSFGSGSTPVIGGNTFNLKASRGNDRNRIVLPFSFAWPRSYTLLVEAWDSSNDTVQPDSIIEKASHSGMINPSRQWQTLKQNTGVAHFEYQIRVTCDDYYYGFGCNKFCRPRDDFFGHYACDQNGNKTCMEGWMGPECNRAICRQGCSPKHGSCKLPGDCRCQYGWQGLYCDKCIPHPGCVHGICNEPWQCLCETNWGGQLCDKDLNYCGTHQPCLNGGTCSNTGPDKYQCSCPEGYSGPNCEIAEHACLSDPCHNRGSCKETSLGFECECSPGWTGPTCSTNIDDCSPNNCSHGGTCQDLVNGFKCVCPPQWTGKTCQLDANECEAKPCVNAKSCKNLIASYYCDCLPGWMGQNCDININDCLGQCQNDASCRDLVNGYRCICPPGYAGDHCERDIDECASNPCLNGGHCQNEINRFQCLCPTGFSGNLCQLDIDYCEPNPCQNGAQCYNRASDYFCKCPEDYEGKNCSHLKDHCRTTPCEVIDSCTVAMASNDTPEGVRYISSNVCGPHGKCKSQSGGKFTCDCNKGFTGTYCHENINDCESNPCRNGGTCIDGVNSYKCICSDGWEGAYCETNINDCSQNPCHNGGTCRDLVNDFYCDCKNGWKGKTCHSRDSQCDEATCNNGGTCYDEGDAFKCMCPGGWEGTTCNIARNSSCLPNPCHNGGTCVVNGESFTCVCKEGWEGPICAQNTNDCSPHPCYNSGTCVDGDNWYRCECAPGFAGPDCRININECQSSPCAFGATCVDEINGYRCVCPPGHSGAKCQEVSGRPCITMGSVIPDGAKWDDDCNTCQCLNGRIACSKVWCGPRPCLLHKGHSECPSGQSCIPILDDQCFVHPCTGVGECRSSSLQPVKTKCTSDSYYQDNCANITFTFNKEMMSPGLTTEHICSELRNLNILKNVSAEYSIYIACEPSPSANNEIHVAISAEDIRDDGNPIKEITDKIIDLVSKRDGNSSLIAAVAEVRVQRRPLKNRTDFLVPLLSSVLTVAWICCLVTAFYWCLRKRRKPGSHTHSASEDNTTNNVREQLNQIKNPIEKHGANTVPIKDYENKNSKMSKIRTHNSEVEEDDMDKHQQKARFAKQPAYTLVDREEKPPNGTPTKHPNWTNKQDNRDLESAQSLNRMEYIV

Target ORF information:

RefSeq Version	NM_000214.3
Organism	Homo sapiens(human)
Definition	Homo sapiens jagged canonical Notch ligand 1 (JAG1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000214.3

ORF Insert Sequence:

ATGCGTTCCC CACGGACGCG CGGCCGGTCC GGGCGCCCCC TAAGCCTCCT GCTCGCCCTG 
CTCTGTGCCC TGCGAGCCAA GGTGTGTGGG GCCTCGGGTC AGTTCGAGTT GGAGATCCTG 
TCCATGCAGA ACGTGAACGG GGAGCTGCAG AACGGGAACT GCTGCGGCGG CGCCCGGAAC 
CCGGGAGACC GCAAGTGCAC CCGCGACGAG TGTGACACAT ACTTCAAAGT GTGCCTCAAG 
GAGTATCAGT CCCGCGTCAC GGCCGGGGGG CCCTGCAGCT TCGGCTCAGG GTCCACGCCT 
GTCATCGGGG GCAACACCTT CAACCTCAAG GCCAGCCGCG GCAACGACCG CAACCGCATC 
GTGCTGCCTT TCAGTTTCGC CTGGCCGAGG TCCTATACGT TGCTTGTGGA GGCGTGGGAT 
TCCAGTAATG ACACCGTTCA ACCTGACAGT ATTATTGAAA AGGCTTCTCA CTCGGGCATG 
ATCAACCCCA GCCGGCAGTG GCAGACGCTG AAGCAGAACA CGGGCGTTGC CCACTTTGAG 
TATCAGATCC GCGTGACCTG TGATGACTAC TACTATGGCT TTGGCTGCAA TAAGTTCTGC 
CGCCCCAGAG ATGACTTCTT TGGACACTAT GCCTGTGACC AGAATGGCAA CAAAACTTGC 
ATGGAAGGCT GGATGGGCCC CGAATGTAAC AGAGCTATTT GCCGACAAGG CTGCAGTCCT 
AAGCATGGGT CTTGCAAACT CCCAGGTGAC TGCAGGTGCC AGTACGGCTG GCAAGGCCTG 
TACTGTGATA AGTGCATCCC ACACCCGGGA TGCGTCCACG GCATCTGTAA TGAGCCCTGG 
CAGTGCCTCT GTGAGACCAA CTGGGGCGGC CAGCTCTGTG ACAAAGATCT CAATTACTGT 
GGGACTCATC AGCCGTGTCT CAACGGGGGA ACTTGTAGCA ACACAGGCCC TGACAAATAT 
CAGTGTTCCT GCCCTGAGGG GTATTCAGGA CCCAACTGTG AAATTGCTGA GCACGCCTGC 
CTCTCTGATC CCTGTCACAA CAGAGGCAGC TGTAAGGAGA CCTCCCTGGG CTTTGAGTGT 
GAGTGTTCCC CAGGCTGGAC CGGCCCCACA TGCTCTACAA ACATTGATGA CTGTTCTCCT 
AATAACTGTT CCCACGGGGG CACCTGCCAG GACCTGGTTA ACGGATTTAA GTGTGTGTGC 
CCCCCACAGT GGACTGGGAA AACGTGCCAG TTAGATGCAA ATGAATGTGA GGCCAAACCT 
TGTGTAAACG CCAAATCCTG TAAGAATCTC ATTGCCAGCT ACTACTGCGA CTGTCTTCCC 
GGCTGGATGG GTCAGAATTG TGACATAAAT ATTAATGACT GCCTTGGCCA GTGTCAGAAT 
GACGCCTCCT GTCGGGATTT GGTTAATGGT TATCGCTGTA TCTGTCCACC TGGCTATGCA 
GGCGATCACT GTGAGAGAGA CATCGATGAA TGTGCCAGCA ACCCCTGTTT GAATGGGGGT 
CACTGTCAGA ATGAAATCAA CAGATTCCAG TGTCTGTGTC CCACTGGTTT CTCTGGAAAC 
CTCTGTCAGC TGGACATCGA TTATTGTGAG CCTAATCCCT GCCAGAACGG TGCCCAGTGC 
TACAACCGTG CCAGTGACTA TTTCTGCAAG TGCCCCGAGG ACTATGAGGG CAAGAACTGC 
TCACACCTGA AAGACCACTG CCGCACGACC CCCTGTGAAG TGATTGACAG CTGCACAGTG 
GCCATGGCTT CCAACGACAC ACCTGAAGGG GTGCGGTATA TTTCCTCCAA CGTCTGTGGT 
CCTCACGGGA AGTGCAAGAG TCAGTCGGGA GGCAAATTCA CCTGTGACTG TAACAAAGGC 
TTCACGGGAA CATACTGCCA TGAAAATATT AATGACTGTG AGAGCAACCC TTGTAGAAAC 
GGTGGCACTT GCATCGATGG TGTCAACTCC TACAAGTGCA TCTGTAGTGA CGGCTGGGAG 
GGGGCCTACT GTGAAACCAA TATTAATGAC TGCAGCCAGA ACCCCTGCCA CAATGGGGGC 
ACGTGTCGCG ACCTGGTCAA TGACTTCTAC TGTGACTGTA AAAATGGGTG GAAAGGAAAG 
ACCTGCCACT CACGTGACAG TCAGTGTGAT GAGGCCACGT GCAACAACGG TGGCACCTGC 
TATGATGAGG GGGATGCTTT TAAGTGCATG TGTCCTGGCG GCTGGGAAGG AACAACCTGT 
AACATAGCCC GAAACAGTAG CTGCCTGCCC AACCCCTGCC ATAATGGGGG CACATGTGTG 
GTCAACGGCG AGTCCTTTAC GTGCGTCTGC AAGGAAGGCT GGGAGGGGCC CATCTGTGCT 
CAGAATACCA ATGACTGCAG CCCTCATCCC TGTTACAACA GCGGCACCTG TGTGGATGGA 
GACAACTGGT ACCGGTGCGA ATGTGCCCCG GGTTTTGCTG GGCCCGACTG CAGAATAAAC 
ATCAATGAAT GCCAGTCTTC ACCTTGTGCC TTTGGAGCGA CCTGTGTGGA TGAGATCAAT 
GGCTACCGGT GTGTCTGCCC TCCAGGGCAC AGTGGTGCCA AGTGCCAGGA AGTTTCAGGG 
AGACCTTGCA TCACCATGGG GAGTGTGATA CCAGATGGGG CCAAATGGGA TGATGACTGT 
AATACCTGCC AGTGCCTGAA TGGACGGATC GCCTGCTCAA AGGTCTGGTG TGGCCCTCGA 
CCTTGCCTGC TCCACAAAGG GCACAGCGAG TGCCCCAGCG GGCAGAGCTG CATCCCCATC 
CTGGACGACC AGTGCTTCGT CCACCCCTGC ACTGGTGTGG GCGAGTGTCG GTCTTCCAGT 
CTCCAGCCGG TGAAGACAAA GTGCACCTCT GACTCCTATT ACCAGGATAA CTGTGCGAAC 
ATCACATTTA CCTTTAACAA GGAGATGATG TCACCAGGTC TTACTACGGA GCACATTTGC 
AGTGAATTGA GGAATTTGAA TATTTTGAAG AATGTTTCCG CTGAATATTC AATCTACATC 
GCTTGCGAGC CTTCCCCTTC AGCGAACAAT GAAATACATG TGGCCATTTC TGCTGAAGAT 
ATACGGGATG ATGGGAACCC GATCAAGGAA ATCACTGACA AAATAATCGA TCTTGTTAGT 
AAACGTGATG GAAACAGCTC GCTGATTGCT GCCGTTGCAG AAGTAAGAGT TCAGAGGCGG 
CCTCTGAAGA ACAGAACAGA TTTCCTTGTT CCCTTGCTGA GCTCTGTCTT AACTGTGGCT 
TGGATCTGTT GCTTGGTGAC GGCCTTCTAC TGGTGCCTGC GGAAGCGGCG GAAGCCGGGC 
AGCCACACAC ACTCAGCCTC TGAGGACAAC ACCACCAACA ACGTGCGGGA GCAGCTGAAC 
CAGATCAAAA ACCCCATTGA GAAACATGGG GCCAACACGG TCCCCATCAA GGATTATGAG 
AACAAGAACT CCAAAATGTC TAAAATAAGG ACACACAATT CTGAAGTAGA AGAGGACGAC 
ATGGACAAAC ACCAGCAGAA AGCCCGGTTT GCCAAGCAGC CGGCGTACAC GCTGGTAGAC 
AGAGAAGAGA AGCCCCCCAA CGGCACGCCG ACAAAACACC CAAACTGGAC AAACAAACAG 
GACAACAGAG ACTTGGAAAG TGCCCAGAGC TTAAACCGAA TGGAGTACAT CGTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

JAG1 ( NM_000214.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_000205.1 Homo sapiens jagged 1 (JAG1), mRNA.

Price & Availability↑

CloneID	OHu23253
Clone ID Related Accession (Same CDS sequence)	NM_000214.3 , NM_000214.2
Accession Version	NM_000214.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3657bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-10
Organism	Homo sapiens(human)
Product	protein jagged-1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BF056748.1, U73936.1, AF028593.1, U77914.1 and AI268246.1. This sequence is a reference standard in the RefSeqGene project. On Feb 22, 2008 this sequence version replaced NM_000214.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF003837.1, BC098393.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: full length.

ATGCGTTCCC CACGGACGCG CGGCCGGTCC GGGCGCCCCC TAAGCCTCCT GCTCGCCCTG 
CTCTGTGCCC TGCGAGCCAA GGTGTGTGGG GCCTCGGGTC AGTTCGAGTT GGAGATCCTG 
TCCATGCAGA ACGTGAACGG GGAGCTGCAG AACGGGAACT GCTGCGGCGG CGCCCGGAAC 
CCGGGAGACC GCAAGTGCAC CCGCGACGAG TGTGACACAT ACTTCAAAGT GTGCCTCAAG 
GAGTATCAGT CCCGCGTCAC GGCCGGGGGG CCCTGCAGCT TCGGCTCAGG GTCCACGCCT 
GTCATCGGGG GCAACACCTT CAACCTCAAG GCCAGCCGCG GCAACGACCG CAACCGCATC 
GTGCTGCCTT TCAGTTTCGC CTGGCCGAGG TCCTATACGT TGCTTGTGGA GGCGTGGGAT 
TCCAGTAATG ACACCGTTCA ACCTGACAGT ATTATTGAAA AGGCTTCTCA CTCGGGCATG 
ATCAACCCCA GCCGGCAGTG GCAGACGCTG AAGCAGAACA CGGGCGTTGC CCACTTTGAG 
TATCAGATCC GCGTGACCTG TGATGACTAC TACTATGGCT TTGGCTGCAA TAAGTTCTGC 
CGCCCCAGAG ATGACTTCTT TGGACACTAT GCCTGTGACC AGAATGGCAA CAAAACTTGC 
ATGGAAGGCT GGATGGGCCC CGAATGTAAC AGAGCTATTT GCCGACAAGG CTGCAGTCCT 
AAGCATGGGT CTTGCAAACT CCCAGGTGAC TGCAGGTGCC AGTACGGCTG GCAAGGCCTG 
TACTGTGATA AGTGCATCCC ACACCCGGGA TGCGTCCACG GCATCTGTAA TGAGCCCTGG 
CAGTGCCTCT GTGAGACCAA CTGGGGCGGC CAGCTCTGTG ACAAAGATCT CAATTACTGT 
GGGACTCATC AGCCGTGTCT CAACGGGGGA ACTTGTAGCA ACACAGGCCC TGACAAATAT 
CAGTGTTCCT GCCCTGAGGG GTATTCAGGA CCCAACTGTG AAATTGCTGA GCACGCCTGC 
CTCTCTGATC CCTGTCACAA CAGAGGCAGC TGTAAGGAGA CCTCCCTGGG CTTTGAGTGT 
GAGTGTTCCC CAGGCTGGAC CGGCCCCACA TGCTCTACAA ACATTGATGA CTGTTCTCCT 
AATAACTGTT CCCACGGGGG CACCTGCCAG GACCTGGTTA ACGGATTTAA GTGTGTGTGC 
CCCCCACAGT GGACTGGGAA AACGTGCCAG TTAGATGCAA ATGAATGTGA GGCCAAACCT 
TGTGTAAACG CCAAATCCTG TAAGAATCTC ATTGCCAGCT ACTACTGCGA CTGTCTTCCC 
GGCTGGATGG GTCAGAATTG TGACATAAAT ATTAATGACT GCCTTGGCCA GTGTCAGAAT 
GACGCCTCCT GTCGGGATTT GGTTAATGGT TATCGCTGTA TCTGTCCACC TGGCTATGCA 
GGCGATCACT GTGAGAGAGA CATCGATGAA TGTGCCAGCA ACCCCTGTTT GAATGGGGGT 
CACTGTCAGA ATGAAATCAA CAGATTCCAG TGTCTGTGTC CCACTGGTTT CTCTGGAAAC 
CTCTGTCAGC TGGACATCGA TTATTGTGAG CCTAATCCCT GCCAGAACGG TGCCCAGTGC 
TACAACCGTG CCAGTGACTA TTTCTGCAAG TGCCCCGAGG ACTATGAGGG CAAGAACTGC 
TCACACCTGA AAGACCACTG CCGCACGACC CCCTGTGAAG TGATTGACAG CTGCACAGTG 
GCCATGGCTT CCAACGACAC ACCTGAAGGG GTGCGGTATA TTTCCTCCAA CGTCTGTGGT 
CCTCACGGGA AGTGCAAGAG TCAGTCGGGA GGCAAATTCA CCTGTGACTG TAACAAAGGC 
TTCACGGGAA CATACTGCCA TGAAAATATT AATGACTGTG AGAGCAACCC TTGTAGAAAC 
GGTGGCACTT GCATCGATGG TGTCAACTCC TACAAGTGCA TCTGTAGTGA CGGCTGGGAG 
GGGGCCTACT GTGAAACCAA TATTAATGAC TGCAGCCAGA ACCCCTGCCA CAATGGGGGC 
ACGTGTCGCG ACCTGGTCAA TGACTTCTAC TGTGACTGTA AAAATGGGTG GAAAGGAAAG 
ACCTGCCACT CACGTGACAG TCAGTGTGAT GAGGCCACGT GCAACAACGG TGGCACCTGC 
TATGATGAGG GGGATGCTTT TAAGTGCATG TGTCCTGGCG GCTGGGAAGG AACAACCTGT 
AACATAGCCC GAAACAGTAG CTGCCTGCCC AACCCCTGCC ATAATGGGGG CACATGTGTG 
GTCAACGGCG AGTCCTTTAC GTGCGTCTGC AAGGAAGGCT GGGAGGGGCC CATCTGTGCT 
CAGAATACCA ATGACTGCAG CCCTCATCCC TGTTACAACA GCGGCACCTG TGTGGATGGA 
GACAACTGGT ACCGGTGCGA ATGTGCCCCG GGTTTTGCTG GGCCCGACTG CAGAATAAAC 
ATCAATGAAT GCCAGTCTTC ACCTTGTGCC TTTGGAGCGA CCTGTGTGGA TGAGATCAAT 
GGCTACCGGT GTGTCTGCCC TCCAGGGCAC AGTGGTGCCA AGTGCCAGGA AGTTTCAGGG 
AGACCTTGCA TCACCATGGG GAGTGTGATA CCAGATGGGG CCAAATGGGA TGATGACTGT 
AATACCTGCC AGTGCCTGAA TGGACGGATC GCCTGCTCAA AGGTCTGGTG TGGCCCTCGA 
CCTTGCCTGC TCCACAAAGG GCACAGCGAG TGCCCCAGCG GGCAGAGCTG CATCCCCATC 
CTGGACGACC AGTGCTTCGT CCACCCCTGC ACTGGTGTGG GCGAGTGTCG GTCTTCCAGT 
CTCCAGCCGG TGAAGACAAA GTGCACCTCT GACTCCTATT ACCAGGATAA CTGTGCGAAC 
ATCACATTTA CCTTTAACAA GGAGATGATG TCACCAGGTC TTACTACGGA GCACATTTGC 
AGTGAATTGA GGAATTTGAA TATTTTGAAG AATGTTTCCG CTGAATATTC AATCTACATC 
GCTTGCGAGC CTTCCCCTTC AGCGAACAAT GAAATACATG TGGCCATTTC TGCTGAAGAT 
ATACGGGATG ATGGGAACCC GATCAAGGAA ATCACTGACA AAATAATCGA TCTTGTTAGT 
AAACGTGATG GAAACAGCTC GCTGATTGCT GCCGTTGCAG AAGTAAGAGT TCAGAGGCGG 
CCTCTGAAGA ACAGAACAGA TTTCCTTGTT CCCTTGCTGA GCTCTGTCTT AACTGTGGCT 
TGGATCTGTT GCTTGGTGAC GGCCTTCTAC TGGTGCCTGC GGAAGCGGCG GAAGCCGGGC 
AGCCACACAC ACTCAGCCTC TGAGGACAAC ACCACCAACA ACGTGCGGGA GCAGCTGAAC 
CAGATCAAAA ACCCCATTGA GAAACATGGG GCCAACACGG TCCCCATCAA GGATTATGAG 
AACAAGAACT CCAAAATGTC TAAAATAAGG ACACACAATT CTGAAGTAGA AGAGGACGAC 
ATGGACAAAC ACCAGCAGAA AGCCCGGTTT GCCAAGCAGC CGGCGTACAC GCTGGTAGAC 
AGAGAAGAGA AGCCCCCCAA CGGCACGCCG ACAAAACACC CAAACTGGAC AAACAAACAG 
GACAACAGAG ACTTGGAAAG TGCCCAGAGC TTAAACCGAA TGGAGTACAT CGTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000205.1
CDS	517..4173
Translation	MRSPRTRGRSGRPLSLLLALLCALRAKVCGASGQFELEILSMQNVNGELQNGNCCGGARNPGDRKCTRDECDTYFKVCLKEYQSRVTAGGPCSFGSGSTPVIGGNTFNLKASRGNDRNRIVLPFSFAWPRSYTLLVEAWDSSNDTVQPDSIIEKASHSGMINPSRQWQTLKQNTGVAHFEYQIRVTCDDYYYGFGCNKFCRPRDDFFGHYACDQNGNKTCMEGWMGPECNRAICRQGCSPKHGSCKLPGDCRCQYGWQGLYCDKCIPHPGCVHGICNEPWQCLCETNWGGQLCDKDLNYCGTHQPCLNGGTCSNTGPDKYQCSCPEGYSGPNCEIAEHACLSDPCHNRGSCKETSLGFECECSPGWTGPTCSTNIDDCSPNNCSHGGTCQDLVNGFKCVCPPQWTGKTCQLDANECEAKPCVNAKSCKNLIASYYCDCLPGWMGQNCDININDCLGQCQNDASCRDLVNGYRCICPPGYAGDHCERDIDECASNPCLNGGHCQNEINRFQCLCPTGFSGNLCQLDIDYCEPNPCQNGAQCYNRASDYFCKCPEDYEGKNCSHLKDHCRTTPCEVIDSCTVAMASNDTPEGVRYISSNVCGPHGKCKSQSGGKFTCDCNKGFTGTYCHENINDCESNPCRNGGTCIDGVNSYKCICSDGWEGAYCETNINDCSQNPCHNGGTCRDLVNDFYCDCKNGWKGKTCHSRDSQCDEATCNNGGTCYDEGDAFKCMCPGGWEGTTCNIARNSSCLPNPCHNGGTCVVNGESFTCVCKEGWEGPICAQNTNDCSPHPCYNSGTCVDGDNWYRCECAPGFAGPDCRININECQSSPCAFGATCVDEINGYRCVCPPGHSGAKCQEVSGRPCITMGSVIPDGAKWDDDCNTCQCLNGRIACSKVWCGPRPCLLHKGHSECPSGQSCIPILDDQCFVHPCTGVGECRSSSLQPVKTKCTSDSYYQDNCANITFTFNKEMMSPGLTTEHICSELRNLNILKNVSAEYSIYIACEPSPSANNEIHVAISAEDIRDDGNPIKEITDKIIDLVSKRDGNSSLIAAVAEVRVQRRPLKNRTDFLVPLLSSVLTVAWICCLVTAFYWCLRKRRKPGSHTHSASEDNTTNNVREQLNQIKNPIEKHGANTVPIKDYENKNSKMSKIRTHNSEVEEDDMDKHQQKARFAKQPAYTLVDREEKPPNGTPTKHPNWTNKQDNRDLESAQSLNRMEYIV

Target ORF information:

RefSeq Version	NM_000214.2
Organism	Homo sapiens(human)
Definition	Homo sapiens jagged 1 (JAG1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000214.2

ORF Insert Sequence:

ATGCGTTCCC CACGGACGCG CGGCCGGTCC GGGCGCCCCC TAAGCCTCCT GCTCGCCCTG 
CTCTGTGCCC TGCGAGCCAA GGTGTGTGGG GCCTCGGGTC AGTTCGAGTT GGAGATCCTG 
TCCATGCAGA ACGTGAACGG GGAGCTGCAG AACGGGAACT GCTGCGGCGG CGCCCGGAAC 
CCGGGAGACC GCAAGTGCAC CCGCGACGAG TGTGACACAT ACTTCAAAGT GTGCCTCAAG 
GAGTATCAGT CCCGCGTCAC GGCCGGGGGG CCCTGCAGCT TCGGCTCAGG GTCCACGCCT 
GTCATCGGGG GCAACACCTT CAACCTCAAG GCCAGCCGCG GCAACGACCG CAACCGCATC 
GTGCTGCCTT TCAGTTTCGC CTGGCCGAGG TCCTATACGT TGCTTGTGGA GGCGTGGGAT 
TCCAGTAATG ACACCGTTCA ACCTGACAGT ATTATTGAAA AGGCTTCTCA CTCGGGCATG 
ATCAACCCCA GCCGGCAGTG GCAGACGCTG AAGCAGAACA CGGGCGTTGC CCACTTTGAG 
TATCAGATCC GCGTGACCTG TGATGACTAC TACTATGGCT TTGGCTGCAA TAAGTTCTGC 
CGCCCCAGAG ATGACTTCTT TGGACACTAT GCCTGTGACC AGAATGGCAA CAAAACTTGC 
ATGGAAGGCT GGATGGGCCC CGAATGTAAC AGAGCTATTT GCCGACAAGG CTGCAGTCCT 
AAGCATGGGT CTTGCAAACT CCCAGGTGAC TGCAGGTGCC AGTACGGCTG GCAAGGCCTG 
TACTGTGATA AGTGCATCCC ACACCCGGGA TGCGTCCACG GCATCTGTAA TGAGCCCTGG 
CAGTGCCTCT GTGAGACCAA CTGGGGCGGC CAGCTCTGTG ACAAAGATCT CAATTACTGT 
GGGACTCATC AGCCGTGTCT CAACGGGGGA ACTTGTAGCA ACACAGGCCC TGACAAATAT 
CAGTGTTCCT GCCCTGAGGG GTATTCAGGA CCCAACTGTG AAATTGCTGA GCACGCCTGC 
CTCTCTGATC CCTGTCACAA CAGAGGCAGC TGTAAGGAGA CCTCCCTGGG CTTTGAGTGT 
GAGTGTTCCC CAGGCTGGAC CGGCCCCACA TGCTCTACAA ACATTGATGA CTGTTCTCCT 
AATAACTGTT CCCACGGGGG CACCTGCCAG GACCTGGTTA ACGGATTTAA GTGTGTGTGC 
CCCCCACAGT GGACTGGGAA AACGTGCCAG TTAGATGCAA ATGAATGTGA GGCCAAACCT 
TGTGTAAACG CCAAATCCTG TAAGAATCTC ATTGCCAGCT ACTACTGCGA CTGTCTTCCC 
GGCTGGATGG GTCAGAATTG TGACATAAAT ATTAATGACT GCCTTGGCCA GTGTCAGAAT 
GACGCCTCCT GTCGGGATTT GGTTAATGGT TATCGCTGTA TCTGTCCACC TGGCTATGCA 
GGCGATCACT GTGAGAGAGA CATCGATGAA TGTGCCAGCA ACCCCTGTTT GAATGGGGGT 
CACTGTCAGA ATGAAATCAA CAGATTCCAG TGTCTGTGTC CCACTGGTTT CTCTGGAAAC 
CTCTGTCAGC TGGACATCGA TTATTGTGAG CCTAATCCCT GCCAGAACGG TGCCCAGTGC 
TACAACCGTG CCAGTGACTA TTTCTGCAAG TGCCCCGAGG ACTATGAGGG CAAGAACTGC 
TCACACCTGA AAGACCACTG CCGCACGACC CCCTGTGAAG TGATTGACAG CTGCACAGTG 
GCCATGGCTT CCAACGACAC ACCTGAAGGG GTGCGGTATA TTTCCTCCAA CGTCTGTGGT 
CCTCACGGGA AGTGCAAGAG TCAGTCGGGA GGCAAATTCA CCTGTGACTG TAACAAAGGC 
TTCACGGGAA CATACTGCCA TGAAAATATT AATGACTGTG AGAGCAACCC TTGTAGAAAC 
GGTGGCACTT GCATCGATGG TGTCAACTCC TACAAGTGCA TCTGTAGTGA CGGCTGGGAG 
GGGGCCTACT GTGAAACCAA TATTAATGAC TGCAGCCAGA ACCCCTGCCA CAATGGGGGC 
ACGTGTCGCG ACCTGGTCAA TGACTTCTAC TGTGACTGTA AAAATGGGTG GAAAGGAAAG 
ACCTGCCACT CACGTGACAG TCAGTGTGAT GAGGCCACGT GCAACAACGG TGGCACCTGC 
TATGATGAGG GGGATGCTTT TAAGTGCATG TGTCCTGGCG GCTGGGAAGG AACAACCTGT 
AACATAGCCC GAAACAGTAG CTGCCTGCCC AACCCCTGCC ATAATGGGGG CACATGTGTG 
GTCAACGGCG AGTCCTTTAC GTGCGTCTGC AAGGAAGGCT GGGAGGGGCC CATCTGTGCT 
CAGAATACCA ATGACTGCAG CCCTCATCCC TGTTACAACA GCGGCACCTG TGTGGATGGA 
GACAACTGGT ACCGGTGCGA ATGTGCCCCG GGTTTTGCTG GGCCCGACTG CAGAATAAAC 
ATCAATGAAT GCCAGTCTTC ACCTTGTGCC TTTGGAGCGA CCTGTGTGGA TGAGATCAAT 
GGCTACCGGT GTGTCTGCCC TCCAGGGCAC AGTGGTGCCA AGTGCCAGGA AGTTTCAGGG 
AGACCTTGCA TCACCATGGG GAGTGTGATA CCAGATGGGG CCAAATGGGA TGATGACTGT 
AATACCTGCC AGTGCCTGAA TGGACGGATC GCCTGCTCAA AGGTCTGGTG TGGCCCTCGA 
CCTTGCCTGC TCCACAAAGG GCACAGCGAG TGCCCCAGCG GGCAGAGCTG CATCCCCATC 
CTGGACGACC AGTGCTTCGT CCACCCCTGC ACTGGTGTGG GCGAGTGTCG GTCTTCCAGT 
CTCCAGCCGG TGAAGACAAA GTGCACCTCT GACTCCTATT ACCAGGATAA CTGTGCGAAC 
ATCACATTTA CCTTTAACAA GGAGATGATG TCACCAGGTC TTACTACGGA GCACATTTGC 
AGTGAATTGA GGAATTTGAA TATTTTGAAG AATGTTTCCG CTGAATATTC AATCTACATC 
GCTTGCGAGC CTTCCCCTTC AGCGAACAAT GAAATACATG TGGCCATTTC TGCTGAAGAT 
ATACGGGATG ATGGGAACCC GATCAAGGAA ATCACTGACA AAATAATCGA TCTTGTTAGT 
AAACGTGATG GAAACAGCTC GCTGATTGCT GCCGTTGCAG AAGTAAGAGT TCAGAGGCGG 
CCTCTGAAGA ACAGAACAGA TTTCCTTGTT CCCTTGCTGA GCTCTGTCTT AACTGTGGCT 
TGGATCTGTT GCTTGGTGAC GGCCTTCTAC TGGTGCCTGC GGAAGCGGCG GAAGCCGGGC 
AGCCACACAC ACTCAGCCTC TGAGGACAAC ACCACCAACA ACGTGCGGGA GCAGCTGAAC 
CAGATCAAAA ACCCCATTGA GAAACATGGG GCCAACACGG TCCCCATCAA GGATTATGAG 
AACAAGAACT CCAAAATGTC TAAAATAAGG ACACACAATT CTGAAGTAGA AGAGGACGAC 
ATGGACAAAC ACCAGCAGAA AGCCCGGTTT GCCAAGCAGC CGGCGTACAC GCTGGTAGAC 
AGAGAAGAGA AGCCCCCCAA CGGCACGCCG ACAAAACACC CAAACTGGAC AAACAAACAG 
GACAACAGAG ACTTGGAAAG TGCCCAGAGC TTAAACCGAA TGGAGTACAT CGTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

JAG1 ( XM_017027707.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016883196.1 Homo sapiens jagged 1 (JAG1), transcript variant X1, mRNA.

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CloneID	OHu92943
Clone ID Related Accession (Same CDS sequence)	XM_017027707.1
Accession Version	XM_017027707.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3180bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	protein jagged-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000020.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCAACC CCAGCCGGCA GTGGCAGACG CTGAAGCAGA ACACGGGCGT TGCCCACTTT 
GAGTATCAGA TCCGCGTGAC CTGTGATGAC TACTACTATG GCTTTGGCTG CAATAAGTTC 
TGCCGCCCCA GAGATGACTT CTTTGGACAC TATGCCTGTG ACCAGAATGG CAACAAAACT 
TGCATGGAAG GCTGGATGGG CCCCGAATGT AACAGAGCTA TTTGCCGACA AGGCTGCAGT 
CCTAAGCATG GGTCTTGCAA ACTCCCAGGT GACTGCAGGT GCCAGTACGG CTGGCAAGGC 
CTGTACTGTG ATAAGTGCAT CCCACACCCG GGATGCGTCC ACGGCATCTG TAATGAGCCC 
TGGCAGTGCC TCTGTGAGAC CAACTGGGGC GGCCAGCTCT GTGACAAAGA TCTCAATTAC 
TGTGGGACTC ATCAGCCGTG TCTCAACGGG GGAACTTGTA GCAACACAGG CCCTGACAAA 
TATCAGTGTT CCTGCCCTGA GGGGTATTCA GGACCCAACT GTGAAATTGC TGAGCACGCC 
TGCCTCTCTG ATCCCTGTCA CAACAGAGGC AGCTGTAAGG AGACCTCCCT GGGCTTTGAG 
TGTGAGTGTT CCCCAGGCTG GACCGGCCCC ACATGCTCTA CAAACATTGA TGACTGTTCT 
CCTAATAACT GTTCCCACGG GGGCACCTGC CAGGACCTGG TTAACGGATT TAAGTGTGTG 
TGCCCCCCAC AGTGGACTGG GAAAACGTGC CAGTTAGATG CAAATGAATG TGAGGCCAAA 
CCTTGTGTAA ACGCCAAATC CTGTAAGAAT CTCATTGCCA GCTACTACTG CGACTGTCTT 
CCCGGCTGGA TGGGTCAGAA TTGTGACATA AATATTAATG ACTGCCTTGG CCAGTGTCAG 
AATGACGCCT CCTGTCGGGA TTTGGTTAAT GGTTATCGCT GTATCTGTCC ACCTGGCTAT 
GCAGGCGATC ACTGTGAGAG AGACATCGAT GAATGTGCCA GCAACCCCTG TTTGAATGGG 
GGTCACTGTC AGAATGAAAT CAACAGATTC CAGTGTCTGT GTCCCACTGG TTTCTCTGGA 
AACCTCTGTC AGCTGGACAT CGATTATTGT GAGCCTAATC CCTGCCAGAA CGGTGCCCAG 
TGCTACAACC GTGCCAGTGA CTATTTCTGC AAGTGCCCCG AGGACTATGA GGGCAAGAAC 
TGCTCACACC TGAAAGACCA CTGCCGCACG ACCCCCTGTG AAGTGATTGA CAGCTGCACA 
GTGGCCATGG CTTCCAACGA CACACCTGAA GGGGTGCGGT ATATTTCCTC CAACGTCTGT 
GGTCCTCACG GGAAGTGCAA GAGTCAGTCG GGAGGCAAAT TCACCTGTGA CTGTAACAAA 
GGCTTCACGG GAACATACTG CCATGAAAAT ATTAATGACT GTGAGAGCAA CCCTTGTAGA 
AACGGTGGCA CTTGCATCGA TGGTGTCAAC TCCTACAAGT GCATCTGTAG TGACGGCTGG 
GAGGGGGCCT ACTGTGAAAC CAATATTAAT GACTGCAGCC AGAACCCCTG CCACAATGGG 
GGCACGTGTC GCGACCTGGT CAATGACTTC TACTGTGACT GTAAAAATGG GTGGAAAGGA 
AAGACCTGCC ACTCACGTGA CAGTCAGTGT GATGAGGCCA CGTGCAACAA CGGTGGCACC 
TGCTATGATG AGGGGGATGC TTTTAAGTGC ATGTGTCCTG GCGGCTGGGA AGGAACAACC 
TGTAACATAG CCCGAAACAG TAGCTGCCTG CCCAACCCCT GCCATAATGG GGGCACATGT 
GTGGTCAACG GCGAGTCCTT TACGTGCGTC TGCAAGGAAG GCTGGGAGGG GCCCATCTGT 
GCTCAGAATA CCAATGACTG CAGCCCTCAT CCCTGTTACA ACAGCGGCAC CTGTGTGGAT 
GGAGACAACT GGTACCGGTG CGAATGTGCC CCGGGTTTTG CTGGGCCCGA CTGCAGAATA 
AACATCAATG AATGCCAGTC TTCACCTTGT GCCTTTGGAG CGACCTGTGT GGATGAGATC 
AATGGCTACC GGTGTGTCTG CCCTCCAGGG CACAGTGGTG CCAAGTGCCA GGAAGTTTCA 
GGGAGACCTT GCATCACCAT GGGGAGTGTG ATACCAGATG GGGCCAAATG GGATGATGAC 
TGTAATACCT GCCAGTGCCT GAATGGACGG ATCGCCTGCT CAAAGGTCTG GTGTGGCCCT 
CGACCTTGCC TGCTCCACAA AGGGCACAGC GAGTGCCCCA GCGGGCAGAG CTGCATCCCC 
ATCCTGGACG ACCAGTGCTT CGTCCACCCC TGCACTGGTG TGGGCGAGTG TCGGTCTTCC 
AGTCTCCAGC CGGTGAAGAC AAAGTGCACC TCTGACTCCT ATTACCAGGA TAACTGTGCG 
AACATCACAT TTACCTTTAA CAAGGAGATG ATGTCACCAG GTCTTACTAC GGAGCACATT 
TGCAGTGAAT TGAGGAATTT GAATATTTTG AAGAATGTTT CCGCTGAATA TTCAATCTAC 
ATCGCTTGCG AGCCTTCCCC TTCAGCGAAC AATGAAATAC ATGTGGCCAT TTCTGCTGAA 
GATATACGGG ATGATGGGAA CCCGATCAAG GAAATCACTG ACAAAATAAT CGATCTTGTT 
AGTAAACGTG ATGGAAACAG CTCGCTGATT GCTGCCGTTG CAGAAGTAAG AGTTCAGAGG 
CGGCCTCTGA AGAACAGAAC AGATTTCCTT GTTCCCTTGC TGAGCTCTGT CTTAACTGTG 
GCTTGGATCT GTTGCTTGGT GACGGCCTTC TACTGGTGCC TGCGGAAGCG GCGGAAGCCG 
GGCAGCCACA CACACTCAGC CTCTGAGGAC AACACCACCA ACAACGTGCG GGAGCAGCTG 
AACCAGATCA AAAACCCCAT TGAGAAACAT GGGGCCAACA CGGTCCCCAT CAAGGATTAT 
GAGAACAAGA ACTCCAAAAT GTCTAAAATA AGGACACACA ATTCTGAAGT AGAAGAGGAC 
GACATGGACA AACACCAGCA GAAAGCCCGG TTTGCCAAGC AGCCGGCGTA CACGCTGGTA 
GACAGAGAAG AGAAGCCCCC CAACGGCACG CCGACAAAAC ACCCAAACTG GACAAACAAA 
CAGGACAACA GAGACTTGGA AAGTGCCCAG AGCTTAAACC GAATGGAGTA CATCGTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016883196.1
CDS	1099..4278
Translation	MINPSRQWQTLKQNTGVAHFEYQIRVTCDDYYYGFGCNKFCRPRDDFFGHYACDQNGNKTCMEGWMGPECNRAICRQGCSPKHGSCKLPGDCRCQYGWQGLYCDKCIPHPGCVHGICNEPWQCLCETNWGGQLCDKDLNYCGTHQPCLNGGTCSNTGPDKYQCSCPEGYSGPNCEIAEHACLSDPCHNRGSCKETSLGFECECSPGWTGPTCSTNIDDCSPNNCSHGGTCQDLVNGFKCVCPPQWTGKTCQLDANECEAKPCVNAKSCKNLIASYYCDCLPGWMGQNCDININDCLGQCQNDASCRDLVNGYRCICPPGYAGDHCERDIDECASNPCLNGGHCQNEINRFQCLCPTGFSGNLCQLDIDYCEPNPCQNGAQCYNRASDYFCKCPEDYEGKNCSHLKDHCRTTPCEVIDSCTVAMASNDTPEGVRYISSNVCGPHGKCKSQSGGKFTCDCNKGFTGTYCHENINDCESNPCRNGGTCIDGVNSYKCICSDGWEGAYCETNINDCSQNPCHNGGTCRDLVNDFYCDCKNGWKGKTCHSRDSQCDEATCNNGGTCYDEGDAFKCMCPGGWEGTTCNIARNSSCLPNPCHNGGTCVVNGESFTCVCKEGWEGPICAQNTNDCSPHPCYNSGTCVDGDNWYRCECAPGFAGPDCRININECQSSPCAFGATCVDEINGYRCVCPPGHSGAKCQEVSGRPCITMGSVIPDGAKWDDDCNTCQCLNGRIACSKVWCGPRPCLLHKGHSECPSGQSCIPILDDQCFVHPCTGVGECRSSSLQPVKTKCTSDSYYQDNCANITFTFNKEMMSPGLTTEHICSELRNLNILKNVSAEYSIYIACEPSPSANNEIHVAISAEDIRDDGNPIKEITDKIIDLVSKRDGNSSLIAAVAEVRVQRRPLKNRTDFLVPLLSSVLTVAWICCLVTAFYWCLRKRRKPGSHTHSASEDNTTNNVREQLNQIKNPIEKHGANTVPIKDYENKNSKMSKIRTHNSEVEEDDMDKHQQKARFAKQPAYTLVDREEKPPNGTPTKHPNWTNKQDNRDLESAQSLNRMEYIV

Target ORF information:

RefSeq Version	XM_017027707.1
Organism	Homo sapiens(human)
Definition	Homo sapiens jagged 1 (JAG1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017027707.1

ORF Insert Sequence:

ATGATCAACC CCAGCCGGCA GTGGCAGACG CTGAAGCAGA ACACGGGCGT TGCCCACTTT 
GAGTATCAGA TCCGCGTGAC CTGTGATGAC TACTACTATG GCTTTGGCTG CAATAAGTTC 
TGCCGCCCCA GAGATGACTT CTTTGGACAC TATGCCTGTG ACCAGAATGG CAACAAAACT 
TGCATGGAAG GCTGGATGGG CCCCGAATGT AACAGAGCTA TTTGCCGACA AGGCTGCAGT 
CCTAAGCATG GGTCTTGCAA ACTCCCAGGT GACTGCAGGT GCCAGTACGG CTGGCAAGGC 
CTGTACTGTG ATAAGTGCAT CCCACACCCG GGATGCGTCC ACGGCATCTG TAATGAGCCC 
TGGCAGTGCC TCTGTGAGAC CAACTGGGGC GGCCAGCTCT GTGACAAAGA TCTCAATTAC 
TGTGGGACTC ATCAGCCGTG TCTCAACGGG GGAACTTGTA GCAACACAGG CCCTGACAAA 
TATCAGTGTT CCTGCCCTGA GGGGTATTCA GGACCCAACT GTGAAATTGC TGAGCACGCC 
TGCCTCTCTG ATCCCTGTCA CAACAGAGGC AGCTGTAAGG AGACCTCCCT GGGCTTTGAG 
TGTGAGTGTT CCCCAGGCTG GACCGGCCCC ACATGCTCTA CAAACATTGA TGACTGTTCT 
CCTAATAACT GTTCCCACGG GGGCACCTGC CAGGACCTGG TTAACGGATT TAAGTGTGTG 
TGCCCCCCAC AGTGGACTGG GAAAACGTGC CAGTTAGATG CAAATGAATG TGAGGCCAAA 
CCTTGTGTAA ACGCCAAATC CTGTAAGAAT CTCATTGCCA GCTACTACTG CGACTGTCTT 
CCCGGCTGGA TGGGTCAGAA TTGTGACATA AATATTAATG ACTGCCTTGG CCAGTGTCAG 
AATGACGCCT CCTGTCGGGA TTTGGTTAAT GGTTATCGCT GTATCTGTCC ACCTGGCTAT 
GCAGGCGATC ACTGTGAGAG AGACATCGAT GAATGTGCCA GCAACCCCTG TTTGAATGGG 
GGTCACTGTC AGAATGAAAT CAACAGATTC CAGTGTCTGT GTCCCACTGG TTTCTCTGGA 
AACCTCTGTC AGCTGGACAT CGATTATTGT GAGCCTAATC CCTGCCAGAA CGGTGCCCAG 
TGCTACAACC GTGCCAGTGA CTATTTCTGC AAGTGCCCCG AGGACTATGA GGGCAAGAAC 
TGCTCACACC TGAAAGACCA CTGCCGCACG ACCCCCTGTG AAGTGATTGA CAGCTGCACA 
GTGGCCATGG CTTCCAACGA CACACCTGAA GGGGTGCGGT ATATTTCCTC CAACGTCTGT 
GGTCCTCACG GGAAGTGCAA GAGTCAGTCG GGAGGCAAAT TCACCTGTGA CTGTAACAAA 
GGCTTCACGG GAACATACTG CCATGAAAAT ATTAATGACT GTGAGAGCAA CCCTTGTAGA 
AACGGTGGCA CTTGCATCGA TGGTGTCAAC TCCTACAAGT GCATCTGTAG TGACGGCTGG 
GAGGGGGCCT ACTGTGAAAC CAATATTAAT GACTGCAGCC AGAACCCCTG CCACAATGGG 
GGCACGTGTC GCGACCTGGT CAATGACTTC TACTGTGACT GTAAAAATGG GTGGAAAGGA 
AAGACCTGCC ACTCACGTGA CAGTCAGTGT GATGAGGCCA CGTGCAACAA CGGTGGCACC 
TGCTATGATG AGGGGGATGC TTTTAAGTGC ATGTGTCCTG GCGGCTGGGA AGGAACAACC 
TGTAACATAG CCCGAAACAG TAGCTGCCTG CCCAACCCCT GCCATAATGG GGGCACATGT 
GTGGTCAACG GCGAGTCCTT TACGTGCGTC TGCAAGGAAG GCTGGGAGGG GCCCATCTGT 
GCTCAGAATA CCAATGACTG CAGCCCTCAT CCCTGTTACA ACAGCGGCAC CTGTGTGGAT 
GGAGACAACT GGTACCGGTG CGAATGTGCC CCGGGTTTTG CTGGGCCCGA CTGCAGAATA 
AACATCAATG AATGCCAGTC TTCACCTTGT GCCTTTGGAG CGACCTGTGT GGATGAGATC 
AATGGCTACC GGTGTGTCTG CCCTCCAGGG CACAGTGGTG CCAAGTGCCA GGAAGTTTCA 
GGGAGACCTT GCATCACCAT GGGGAGTGTG ATACCAGATG GGGCCAAATG GGATGATGAC 
TGTAATACCT GCCAGTGCCT GAATGGACGG ATCGCCTGCT CAAAGGTCTG GTGTGGCCCT 
CGACCTTGCC TGCTCCACAA AGGGCACAGC GAGTGCCCCA GCGGGCAGAG CTGCATCCCC 
ATCCTGGACG ACCAGTGCTT CGTCCACCCC TGCACTGGTG TGGGCGAGTG TCGGTCTTCC 
AGTCTCCAGC CGGTGAAGAC AAAGTGCACC TCTGACTCCT ATTACCAGGA TAACTGTGCG 
AACATCACAT TTACCTTTAA CAAGGAGATG ATGTCACCAG GTCTTACTAC GGAGCACATT 
TGCAGTGAAT TGAGGAATTT GAATATTTTG AAGAATGTTT CCGCTGAATA TTCAATCTAC 
ATCGCTTGCG AGCCTTCCCC TTCAGCGAAC AATGAAATAC ATGTGGCCAT TTCTGCTGAA 
GATATACGGG ATGATGGGAA CCCGATCAAG GAAATCACTG ACAAAATAAT CGATCTTGTT 
AGTAAACGTG ATGGAAACAG CTCGCTGATT GCTGCCGTTG CAGAAGTAAG AGTTCAGAGG 
CGGCCTCTGA AGAACAGAAC AGATTTCCTT GTTCCCTTGC TGAGCTCTGT CTTAACTGTG 
GCTTGGATCT GTTGCTTGGT GACGGCCTTC TACTGGTGCC TGCGGAAGCG GCGGAAGCCG 
GGCAGCCACA CACACTCAGC CTCTGAGGAC AACACCACCA ACAACGTGCG GGAGCAGCTG 
AACCAGATCA AAAACCCCAT TGAGAAACAT GGGGCCAACA CGGTCCCCAT CAAGGATTAT 
GAGAACAAGA ACTCCAAAAT GTCTAAAATA AGGACACACA ATTCTGAAGT AGAAGAGGAC 
GACATGGACA AACACCAGCA GAAAGCCCGG TTTGCCAAGC AGCCGGCGTA CACGCTGGTA 
GACAGAGAAG AGAAGCCCCC CAACGGCACG CCGACAAAAC ACCCAAACTG GACAAACAAA 
CAGGACAACA GAGACTTGGA AAGTGCCCAG AGCTTAAACC GAATGGAGTA CATCGTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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JAG1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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