AFP cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	AFP
Entrez Gene ID	174
Full Name	alpha fetoprotein
Synonyms	AFPD,FETA,HPAFP
General protein information	Preferred Names alpha fetoprotein Names alpha-fetoprotein alpha-1-fetoprotein alpha-fetoglobulin
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 4 Map Location: 4q13.3
Summary	This gene encodes alpha-fetoprotein, a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha-fetoprotein expression in adults is often associated with hepatoma or teratoma. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha-fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha-fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha-fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly. [provided by RefSeq, Jul 2008].
Disorder MIM:	104150
Disorder Html:	[Hereditary persistence of alpha-fetoprotein], 615970 (3); Alpha-fetoprotein deficiency, 615969 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001134.2	NP_001125.1	alpha-fetoprotein isoform 1 precursor
NM_001354717.1	NP_001341646.1	alpha-fetoprotein isoform 2
NM_001354717.2	NP_001341646.2	alpha-fetoprotein isoform 2
NM_001134.3	NP_001125.1	alpha-fetoprotein isoform 1 precursor

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	AFP	NP_001003027.1
Gallus gallus (chicken)	AFP	XP_003641248.2
Pan troglodytes (chimpanzee)	AFP	XP_517231.2
Bos taurus (cattle)	AFP	NP_001029434.1
Mus musculus (house mouse)	Afp	NP_031449.3
	AFP	XP_001103873.1
Rattus norvegicus (Norway rat)	Afp	NP_036625.2
Homo sapiens (human)	AFP	NP_001125.1

Gene Ontology

The following AFP gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AFP cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu21468	NM_001134.2 Latest version!	Homo sapiens alpha fetoprotein (AFP), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu103052	NM_001354717.1 Latest version!	Homo sapiens alpha fetoprotein (AFP), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OHu108518	NM_001354717.2 Latest version!	Homo sapiens alpha fetoprotein (AFP), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OHu108519	NM_001134.3 Latest version!	Homo sapiens alpha fetoprotein (AFP), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

AFP ( NM_001134.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001125.1 Homo sapiens alpha fetoprotein (AFP), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu21468
Clone ID Related Accession (Same CDS sequence)	NM_001134.2
Accession Version	NM_001134.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1830bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-23
Organism	Homo sapiens(human)
Product	alpha-fetoprotein isoform 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from V01514.1 and AI336986.1. This sequence is a reference standard in the RefSeqGene project. On Sep 4, 2013 this sequence version replaced NM_001134.1. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: V01514.1, BC027881.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03267750, SAMN03267752 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

ATGAAGTGGG TGGAATCAAT TTTTTTAATT TTCCTACTAA ATTTTACTGA ATCCAGAACA 
CTGCATAGAA ATGAATATGG AATAGCTTCC ATATTGGATT CTTACCAATG TACTGCAGAG 
ATAAGTTTAG CTGACCTGGC TACCATATTT TTTGCCCAGT TTGTTCAAGA AGCCACTTAC 
AAGGAAGTAA GCAAAATGGT GAAAGATGCA TTGACTGCAA TTGAGAAACC CACTGGAGAT 
GAACAGTCTT CAGGGTGTTT AGAAAACCAG CTACCTGCCT TTCTGGAAGA ACTTTGCCAT 
GAGAAAGAAA TTTTGGAGAA GTACGGACAT TCAGACTGCT GCAGCCAAAG TGAAGAGGGA 
AGACATAACT GTTTTCTTGC ACACAAAAAG CCCACTCCAG CATCGATCCC ACTTTTCCAA 
GTTCCAGAAC CTGTCACAAG CTGTGAAGCA TATGAAGAAG ACAGGGAGAC ATTCATGAAC 
AAATTCATTT ATGAGATAGC AAGAAGGCAT CCCTTCCTGT ATGCACCTAC AATTCTTCTT 
TGGGCTGCTC GCTATGACAA AATAATTCCA TCTTGCTGCA AAGCTGAAAA TGCAGTTGAA 
TGCTTCCAAA CAAAGGCAGC AACAGTTACA AAAGAATTAA GAGAAAGCAG CTTGTTAAAT 
CAACATGCAT GTGCAGTAAT GAAAAATTTT GGGACCCGAA CTTTCCAAGC CATAACTGTT 
ACTAAACTGA GTCAGAAGTT TACCAAAGTT AATTTTACTG AAATCCAGAA ACTAGTCCTG 
GATGTGGCCC ATGTACATGA GCACTGTTGC AGAGGAGATG TGCTGGATTG TCTGCAGGAT 
GGGGAAAAAA TCATGTCCTA CATATGTTCT CAACAAGACA CTCTGTCAAA CAAAATAACA 
GAATGCTGCA AACTGACCAC GCTGGAACGT GGTCAATGTA TAATTCATGC AGAAAATGAT 
GAAAAACCTG AAGGTCTATC TCCAAATCTA AACAGGTTTT TAGGAGATAG AGATTTTAAC 
CAATTTTCTT CAGGGGAAAA AAATATCTTC TTGGCAAGTT TTGTTCATGA ATATTCAAGA 
AGACATCCTC AGCTTGCTGT CTCAGTAATT CTAAGAGTTG CTAAAGGATA CCAGGAGTTA 
TTGGAGAAGT GTTTCCAGAC TGAAAACCCT CTTGAATGCC AAGATAAAGG AGAAGAAGAA 
TTACAGAAAT ACATCCAGGA GAGCCAAGCA TTGGCAAAGC GAAGCTGCGG CCTCTTCCAG 
AAACTAGGAG AATATTACTT ACAAAATGCG TTTCTCGTTG CTTACACAAA GAAAGCCCCC 
CAGCTGACCT CGTCGGAGCT GATGGCCATC ACCAGAAAAA TGGCAGCCAC AGCAGCCACT 
TGTTGCCAAC TCAGTGAGGA CAAACTATTG GCCTGTGGCG AGGGAGCGGC TGACATTATT 
ATCGGACACT TATGTATCAG ACATGAAATG ACTCCAGTAA ACCCTGGTGT TGGCCAGTGC 
TGCACTTCTT CATATGCCAA CAGGAGGCCA TGCTTCAGCA GCTTGGTGGT GGATGAAACA 
TATGTCCCTC CTGCATTCTC TGATGACAAG TTCATTTTCC ATAAGGATCT GTGCCAAGCT 
CAGGGTGTAG CGCTGCAAAC GATGAAGCAA GAGTTTCTCA TTAACCTTGT GAAGCAAAAG 
CCACAAATAA CAGAGGAACA ACTTGAGGCT GTCATTGCAG ATTTCTCAGG CCTGTTGGAG 
AAATGCTGCC AAGGCCAGGA ACAGGAAGTC TGCTTTGCTG AAGAGGGACA AAAACTGATT 
TCAAAAACTC GTGCTGCTTT GGGAGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001125.1
CDS	45..1874
Translation	MKWVESIFLIFLLNFTESRTLHRNEYGIASILDSYQCTAEISLADLATIFFAQFVQEATYKEVSKMVKDALTAIEKPTGDEQSSGCLENQLPAFLEELCHEKEILEKYGHSDCCSQSEEGRHNCFLAHKKPTPASIPLFQVPEPVTSCEAYEEDRETFMNKFIYEIARRHPFLYAPTILLWAARYDKIIPSCCKAENAVECFQTKAATVTKELRESSLLNQHACAVMKNFGTRTFQAITVTKLSQKFTKVNFTEIQKLVLDVAHVHEHCCRGDVLDCLQDGEKIMSYICSQQDTLSNKITECCKLTTLERGQCIIHAENDEKPEGLSPNLNRFLGDRDFNQFSSGEKNIFLASFVHEYSRRHPQLAVSVILRVAKGYQELLEKCFQTENPLECQDKGEEELQKYIQESQALAKRSCGLFQKLGEYYLQNAFLVAYTKKAPQLTSSELMAITRKMAATAATCCQLSEDKLLACGEGAADIIIGHLCIRHEMTPVNPGVGQCCTSSYANRRPCFSSLVVDETYVPPAFSDDKFIFHKDLCQAQGVALQTMKQEFLINLVKQKPQITEEQLEAVIADFSGLLEKCCQGQEQEVCFAEEGQKLISKTRAALGV

Target ORF information:

RefSeq Version	NM_001134.2
Organism	Homo sapiens(human)
Definition	Homo sapiens alpha fetoprotein (AFP), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001134.2

ORF Insert Sequence:

ATGAAGTGGG TGGAATCAAT TTTTTTAATT TTCCTACTAA ATTTTACTGA ATCCAGAACA 
CTGCATAGAA ATGAATATGG AATAGCTTCC ATATTGGATT CTTACCAATG TACTGCAGAG 
ATAAGTTTAG CTGACCTGGC TACCATATTT TTTGCCCAGT TTGTTCAAGA AGCCACTTAC 
AAGGAAGTAA GCAAAATGGT GAAAGATGCA TTGACTGCAA TTGAGAAACC CACTGGAGAT 
GAACAGTCTT CAGGGTGTTT AGAAAACCAG CTACCTGCCT TTCTGGAAGA ACTTTGCCAT 
GAGAAAGAAA TTTTGGAGAA GTACGGACAT TCAGACTGCT GCAGCCAAAG TGAAGAGGGA 
AGACATAACT GTTTTCTTGC ACACAAAAAG CCCACTCCAG CATCGATCCC ACTTTTCCAA 
GTTCCAGAAC CTGTCACAAG CTGTGAAGCA TATGAAGAAG ACAGGGAGAC ATTCATGAAC 
AAATTCATTT ATGAGATAGC AAGAAGGCAT CCCTTCCTGT ATGCACCTAC AATTCTTCTT 
TGGGCTGCTC GCTATGACAA AATAATTCCA TCTTGCTGCA AAGCTGAAAA TGCAGTTGAA 
TGCTTCCAAA CAAAGGCAGC AACAGTTACA AAAGAATTAA GAGAAAGCAG CTTGTTAAAT 
CAACATGCAT GTGCAGTAAT GAAAAATTTT GGGACCCGAA CTTTCCAAGC CATAACTGTT 
ACTAAACTGA GTCAGAAGTT TACCAAAGTT AATTTTACTG AAATCCAGAA ACTAGTCCTG 
GATGTGGCCC ATGTACATGA GCACTGTTGC AGAGGAGATG TGCTGGATTG TCTGCAGGAT 
GGGGAAAAAA TCATGTCCTA CATATGTTCT CAACAAGACA CTCTGTCAAA CAAAATAACA 
GAATGCTGCA AACTGACCAC GCTGGAACGT GGTCAATGTA TAATTCATGC AGAAAATGAT 
GAAAAACCTG AAGGTCTATC TCCAAATCTA AACAGGTTTT TAGGAGATAG AGATTTTAAC 
CAATTTTCTT CAGGGGAAAA AAATATCTTC TTGGCAAGTT TTGTTCATGA ATATTCAAGA 
AGACATCCTC AGCTTGCTGT CTCAGTAATT CTAAGAGTTG CTAAAGGATA CCAGGAGTTA 
TTGGAGAAGT GTTTCCAGAC TGAAAACCCT CTTGAATGCC AAGATAAAGG AGAAGAAGAA 
TTACAGAAAT ACATCCAGGA GAGCCAAGCA TTGGCAAAGC GAAGCTGCGG CCTCTTCCAG 
AAACTAGGAG AATATTACTT ACAAAATGCG TTTCTCGTTG CTTACACAAA GAAAGCCCCC 
CAGCTGACCT CGTCGGAGCT GATGGCCATC ACCAGAAAAA TGGCAGCCAC AGCAGCCACT 
TGTTGCCAAC TCAGTGAGGA CAAACTATTG GCCTGTGGCG AGGGAGCGGC TGACATTATT 
ATCGGACACT TATGTATCAG ACATGAAATG ACTCCAGTAA ACCCTGGTGT TGGCCAGTGC 
TGCACTTCTT CATATGCCAA CAGGAGGCCA TGCTTCAGCA GCTTGGTGGT GGATGAAACA 
TATGTCCCTC CTGCATTCTC TGATGACAAG TTCATTTTCC ATAAGGATCT GTGCCAAGCT 
CAGGGTGTAG CGCTGCAAAC GATGAAGCAA GAGTTTCTCA TTAACCTTGT GAAGCAAAAG 
CCACAAATAA CAGAGGAACA ACTTGAGGCT GTCATTGCAG ATTTCTCAGG CCTGTTGGAG 
AAATGCTGCC AAGGCCAGGA ACAGGAAGTC TGCTTTGCTG AAGAGGGACA AAAACTGATT 
TCAAAAACTC GTGCTGCTTT GGGAGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFP ( NM_001354717.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001341646.1 Homo sapiens alpha fetoprotein (AFP), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu103052
Clone ID Related Accession (Same CDS sequence)	NM_001354717.1
Accession Version	NM_001354717.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1356bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-25
Organism	Homo sapiens(human)
Product	alpha-fetoprotein isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC108157.3 and AK297674.1. Transcript Variant: This variant (2) differs in the 5' UTR and coding sequence compared to variant 1. The resulting isoform (2) is shorter at the N-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK297674.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN03267750, SAMN03267752 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAACAAAT TCATTTATGA GATAGCAAGA AGGCATCCCT TCCTGTATGC ACCTACAATT 
CTTCTTTGGG CTGCTCGCTA TGACAAAATA ATTCCATCTT GCTGCAAAGC TGAAAATGCA 
GTTGAATGCT TCCAAACAAA GGCAGCAACA GTTACAAAAG AATTAAGAGA AAGCAGCTTG 
TTAAATCAAC ATGCATGTGC AGTAATGAAA AATTTTGGGA CCCGAACTTT CCAAGCCATA 
ACTGTTACTA AACTGAGTCA GAAGTTTACC AAAGTTAATT TTACTGAAAT CCAGAAACTA 
GTCCTGGATG TGGCCCATGT ACATGAGCAC TGTTGCAGAG GAGATGTGCT GGATTGTCTG 
CAGGATGGGG AAAGAATCAT GTCCTACATA TGTTCTCAAC AAGACACTCT GTCAAACAAA 
ATAACAGAAT GCTGCAAACT GACCACGCTG GAACGTGGTC AATGTATAAT TCATGCAGAA 
AATGATGAAA AACCTGAAGG TCTATCTCCA AATCTAAACA GGTTTTTAGG AGATAGAGAT 
TTTAACCAAT TTTCTTCAGG GGAAAAAAAT ATCTTCTTGG CAAGTTTTGT TCATGAATAT 
TCAAGAAGAC ATCCTCAGCT TGCTGTCTCA GTAATTCTAA GAGTTGCTAA AGGATACCAG 
GAGTTATTGG AGAAGTGTTT CCAGACTGAA AACCCTCTTG AATGCCAAGA TAAAGGAGAA 
GAAGAATTAC AGAAATACAT CCAGGAGAGC CAAGCATTGG CAAAGCGAAG CTGCGGCCTC 
TTCCAGAAAC TAGGAGAATA TTACTTACAA AATGCGTTTC TCGTTGCTTA CACAAAGAAA 
GCCCCCCAGC TGACCTCGTC GGAGCTGATG GCCATCACCA GAAAAATGGC AGCCACAGCA 
GCCACTTGTT GCCAACTCAG TGAGGACAAA CTATTGGCCT GTGGCGAGGG AGCGGCTGAC 
ATTATTATCG GACACTTATG TATCAGACAT GAAATGACTC CAGTAAACCC TGGTGTTGGC 
CAGTGCTGCA CTTCTTCATA TGCCAACAGG AGGCCATGCT TCAGCAGCTT GGTGGTGGAT 
GAAACATATG TCCCTCCTGC ATTCTCTGAT GACAAGTTCA TTTTCCATAA GGATCTGTGC 
CAAGCTCAGG GTGTAGCGCT GCAAACGATG AAGCAAGAGT TTCTCATTAA CCTTGTGAAG 
CAAAAGCCAC AAATAACAGA GGAACAACTT GAGGCTGTCA TTGCAGATTT CTCAGGCCTG 
TTGGAGAAAT GCTGCCAAGG CCAGGAACAG GAAGTCTGCT TTGCTGAAGA GGGACAAAAA 
CTGATTTCAA AAACTCGTGC TGCTTTGGGA GTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001341646.1
CDS	377..1732
Translation	MNKFIYEIARRHPFLYAPTILLWAARYDKIIPSCCKAENAVECFQTKAATVTKELRESSLLNQHACAVMKNFGTRTFQAITVTKLSQKFTKVNFTEIQKLVLDVAHVHEHCCRGDVLDCLQDGERIMSYICSQQDTLSNKITECCKLTTLERGQCIIHAENDEKPEGLSPNLNRFLGDRDFNQFSSGEKNIFLASFVHEYSRRHPQLAVSVILRVAKGYQELLEKCFQTENPLECQDKGEEELQKYIQESQALAKRSCGLFQKLGEYYLQNAFLVAYTKKAPQLTSSELMAITRKMAATAATCCQLSEDKLLACGEGAADIIIGHLCIRHEMTPVNPGVGQCCTSSYANRRPCFSSLVVDETYVPPAFSDDKFIFHKDLCQAQGVALQTMKQEFLINLVKQKPQITEEQLEAVIADFSGLLEKCCQGQEQEVCFAEEGQKLISKTRAALGV

Target ORF information:

RefSeq Version	NM_001354717.1
Organism	Homo sapiens(human)
Definition	Homo sapiens alpha fetoprotein (AFP), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001354717.1

ORF Insert Sequence:

ATGAACAAAT TCATTTATGA GATAGCAAGA AGGCATCCCT TCCTGTATGC ACCTACAATT 
CTTCTTTGGG CTGCTCGCTA TGACAAAATA ATTCCATCTT GCTGCAAAGC TGAAAATGCA 
GTTGAATGCT TCCAAACAAA GGCAGCAACA GTTACAAAAG AATTAAGAGA AAGCAGCTTG 
TTAAATCAAC ATGCATGTGC AGTAATGAAA AATTTTGGGA CCCGAACTTT CCAAGCCATA 
ACTGTTACTA AACTGAGTCA GAAGTTTACC AAAGTTAATT TTACTGAAAT CCAGAAACTA 
GTCCTGGATG TGGCCCATGT ACATGAGCAC TGTTGCAGAG GAGATGTGCT GGATTGTCTG 
CAGGATGGGG AAAGAATCAT GTCCTACATA TGTTCTCAAC AAGACACTCT GTCAAACAAA 
ATAACAGAAT GCTGCAAACT GACCACGCTG GAACGTGGTC AATGTATAAT TCATGCAGAA 
AATGATGAAA AACCTGAAGG TCTATCTCCA AATCTAAACA GGTTTTTAGG AGATAGAGAT 
TTTAACCAAT TTTCTTCAGG GGAAAAAAAT ATCTTCTTGG CAAGTTTTGT TCATGAATAT 
TCAAGAAGAC ATCCTCAGCT TGCTGTCTCA GTAATTCTAA GAGTTGCTAA AGGATACCAG 
GAGTTATTGG AGAAGTGTTT CCAGACTGAA AACCCTCTTG AATGCCAAGA TAAAGGAGAA 
GAAGAATTAC AGAAATACAT CCAGGAGAGC CAAGCATTGG CAAAGCGAAG CTGCGGCCTC 
TTCCAGAAAC TAGGAGAATA TTACTTACAA AATGCGTTTC TCGTTGCTTA CACAAAGAAA 
GCCCCCCAGC TGACCTCGTC GGAGCTGATG GCCATCACCA GAAAAATGGC AGCCACAGCA 
GCCACTTGTT GCCAACTCAG TGAGGACAAA CTATTGGCCT GTGGCGAGGG AGCGGCTGAC 
ATTATTATCG GACACTTATG TATCAGACAT GAAATGACTC CAGTAAACCC TGGTGTTGGC 
CAGTGCTGCA CTTCTTCATA TGCCAACAGG AGGCCATGCT TCAGCAGCTT GGTGGTGGAT 
GAAACATATG TCCCTCCTGC ATTCTCTGAT GACAAGTTCA TTTTCCATAA GGATCTGTGC 
CAAGCTCAGG GTGTAGCGCT GCAAACGATG AAGCAAGAGT TTCTCATTAA CCTTGTGAAG 
CAAAAGCCAC AAATAACAGA GGAACAACTT GAGGCTGTCA TTGCAGATTT CTCAGGCCTG 
TTGGAGAAAT GCTGCCAAGG CCAGGAACAG GAAGTCTGCT TTGCTGAAGA GGGACAAAAA 
CTGATTTCAA AAACTCGTGC TGCTTTGGGA GTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFP ( NM_001354717.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001341646.2 Homo sapiens alpha fetoprotein (AFP), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu108518
Clone ID Related Accession (Same CDS sequence)	NM_001354717.2
Accession Version	NM_001354717.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1356bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-03
Organism	Homo sapiens(human)
Product	alpha-fetoprotein isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC108157.3. On May 31, 2019 this sequence version replaced NM_001354717.1. Transcript Variant: This variant (2) differs in the 5' UTR and coding sequence compared to variant 1. The resulting isoform (2) is shorter at the N-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK297674.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN03267750, SAMN03267752 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAACAAAT TCATTTATGA GATAGCAAGA AGGCATCCCT TCCTGTATGC ACCTACAATT 
CTTCTTTGGG CTGCTCGCTA TGACAAAATA ATTCCATCTT GCTGCAAAGC TGAAAATGCA 
GTTGAATGCT TCCAAACAAA GGCAGCAACA GTTACAAAAG AATTAAGAGA AAGCAGCTTG 
TTAAATCAAC ATGCATGTGC AGTAATGAAA AATTTTGGGA CCCGAACTTT CCAAGCCATA 
ACTGTTACTA AACTGAGTCA GAAGTTTACC AAAGTTAATT TTACTGAAAT CCAGAAACTA 
GTCCTGGATG TGGCCCATGT ACATGAGCAC TGTTGCAGAG GAGATGTGCT GGATTGTCTG 
CAGGATGGGG AAAAAATCAT GTCCTACATA TGTTCTCAAC AAGACACTCT GTCAAACAAA 
ATAACAGAAT GCTGCAAACT GACCACGCTG GAACGTGGTC AATGTATAAT TCATGCAGAA 
AATGATGAAA AACCTGAAGG TCTATCTCCA AATCTAAACA GGTTTTTAGG AGATAGAGAT 
TTTAACCAAT TTTCTTCAGG GGAAAAAAAT ATCTTCTTGG CAAGTTTTGT TCATGAATAT 
TCAAGAAGAC ATCCTCAGCT TGCTGTCTCA GTAATTCTAA GAGTTGCTAA AGGATACCAG 
GAGTTATTGG AGAAGTGTTT CCAGACTGAA AACCCTCTTG AATGCCAAGA TAAAGGAGAA 
GAAGAATTAC AGAAATACAT CCAGGAGAGC CAAGCATTGG CAAAGCGAAG CTGCGGCCTC 
TTCCAGAAAC TAGGAGAATA TTACTTACAA AATGCGTTTC TCGTTGCTTA CACAAAGAAA 
GCCCCCCAGC TGACCTCGTC GGAGCTGATG GCCATCACCA GAAAAATGGC AGCCACAGCA 
GCCACTTGTT GCCAACTCAG TGAGGACAAA CTATTGGCCT GTGGCGAGGG AGCGGCTGAC 
ATTATTATCG GACACTTATG TATCAGACAT GAAATGACTC CAGTAAACCC TGGTGTTGGC 
CAGTGCTGCA CTTCTTCATA TGCCAACAGG AGGCCATGCT TCAGCAGCTT GGTGGTGGAT 
GAAACATATG TCCCTCCTGC ATTCTCTGAT GACAAGTTCA TTTTCCATAA GGATCTGTGC 
CAAGCTCAGG GTGTAGCGCT GCAAACAATG AAGCAAGAGT TTCTCATTAA CCTTGTGAAG 
CAAAAGCCAC AAATAACAGA GGAACAACTT GAGGCTGTCA TTGCAGATTT CTCAGGCCTG 
TTGGAGAAAT GCTGCCAAGG CCAGGAACAG GAAGTCTGCT TTGCTGAAGA GGGACAAAAA 
CTGATTTCAA AAACTCGTGC TGCTTTGGGA GTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001341646.2
CDS	375..1730
Translation	MNKFIYEIARRHPFLYAPTILLWAARYDKIIPSCCKAENAVECFQTKAATVTKELRESSLLNQHACAVMKNFGTRTFQAITVTKLSQKFTKVNFTEIQKLVLDVAHVHEHCCRGDVLDCLQDGEKIMSYICSQQDTLSNKITECCKLTTLERGQCIIHAENDEKPEGLSPNLNRFLGDRDFNQFSSGEKNIFLASFVHEYSRRHPQLAVSVILRVAKGYQELLEKCFQTENPLECQDKGEEELQKYIQESQALAKRSCGLFQKLGEYYLQNAFLVAYTKKAPQLTSSELMAITRKMAATAATCCQLSEDKLLACGEGAADIIIGHLCIRHEMTPVNPGVGQCCTSSYANRRPCFSSLVVDETYVPPAFSDDKFIFHKDLCQAQGVALQTMKQEFLINLVKQKPQITEEQLEAVIADFSGLLEKCCQGQEQEVCFAEEGQKLISKTRAALGV

Target ORF information:

RefSeq Version	NM_001354717.2
Organism	Homo sapiens(human)
Definition	Homo sapiens alpha fetoprotein (AFP), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001354717.2

ORF Insert Sequence:

ATGAACAAAT TCATTTATGA GATAGCAAGA AGGCATCCCT TCCTGTATGC ACCTACAATT 
CTTCTTTGGG CTGCTCGCTA TGACAAAATA ATTCCATCTT GCTGCAAAGC TGAAAATGCA 
GTTGAATGCT TCCAAACAAA GGCAGCAACA GTTACAAAAG AATTAAGAGA AAGCAGCTTG 
TTAAATCAAC ATGCATGTGC AGTAATGAAA AATTTTGGGA CCCGAACTTT CCAAGCCATA 
ACTGTTACTA AACTGAGTCA GAAGTTTACC AAAGTTAATT TTACTGAAAT CCAGAAACTA 
GTCCTGGATG TGGCCCATGT ACATGAGCAC TGTTGCAGAG GAGATGTGCT GGATTGTCTG 
CAGGATGGGG AAAAAATCAT GTCCTACATA TGTTCTCAAC AAGACACTCT GTCAAACAAA 
ATAACAGAAT GCTGCAAACT GACCACGCTG GAACGTGGTC AATGTATAAT TCATGCAGAA 
AATGATGAAA AACCTGAAGG TCTATCTCCA AATCTAAACA GGTTTTTAGG AGATAGAGAT 
TTTAACCAAT TTTCTTCAGG GGAAAAAAAT ATCTTCTTGG CAAGTTTTGT TCATGAATAT 
TCAAGAAGAC ATCCTCAGCT TGCTGTCTCA GTAATTCTAA GAGTTGCTAA AGGATACCAG 
GAGTTATTGG AGAAGTGTTT CCAGACTGAA AACCCTCTTG AATGCCAAGA TAAAGGAGAA 
GAAGAATTAC AGAAATACAT CCAGGAGAGC CAAGCATTGG CAAAGCGAAG CTGCGGCCTC 
TTCCAGAAAC TAGGAGAATA TTACTTACAA AATGCGTTTC TCGTTGCTTA CACAAAGAAA 
GCCCCCCAGC TGACCTCGTC GGAGCTGATG GCCATCACCA GAAAAATGGC AGCCACAGCA 
GCCACTTGTT GCCAACTCAG TGAGGACAAA CTATTGGCCT GTGGCGAGGG AGCGGCTGAC 
ATTATTATCG GACACTTATG TATCAGACAT GAAATGACTC CAGTAAACCC TGGTGTTGGC 
CAGTGCTGCA CTTCTTCATA TGCCAACAGG AGGCCATGCT TCAGCAGCTT GGTGGTGGAT 
GAAACATATG TCCCTCCTGC ATTCTCTGAT GACAAGTTCA TTTTCCATAA GGATCTGTGC 
CAAGCTCAGG GTGTAGCGCT GCAAACAATG AAGCAAGAGT TTCTCATTAA CCTTGTGAAG 
CAAAAGCCAC AAATAACAGA GGAACAACTT GAGGCTGTCA TTGCAGATTT CTCAGGCCTG 
TTGGAGAAAT GCTGCCAAGG CCAGGAACAG GAAGTCTGCT TTGCTGAAGA GGGACAAAAA 
CTGATTTCAA AAACTCGTGC TGCTTTGGGA GTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFP ( NM_001134.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001125.1 Homo sapiens alpha fetoprotein (AFP), transcript variant 1, mRNA.

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CloneID	OHu108519
Clone ID Related Accession (Same CDS sequence)	NM_001134.3
Accession Version	NM_001134.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1830bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-01
Organism	Homo sapiens(human)
Product	alpha-fetoprotein isoform 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC108157.3. On May 17, 2019 this sequence version replaced NM_001134.2. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: V01514.1, BC027881.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03267750, SAMN03267752 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000395792.7/ ENSP00000379138.2 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGAAGTGGG TGGAATCAAT TTTTTTAATT TTCCTACTAA ATTTTACTGA ATCCAGAACA 
CTGCATAGAA ATGAATATGG AATAGCTTCC ATATTGGATT CTTACCAATG TACTGCAGAG 
ATAAGTTTAG CTGACCTGGC TACCATATTT TTTGCCCAGT TTGTTCAAGA AGCCACTTAC 
AAGGAAGTAA GCAAAATGGT GAAAGATGCA TTGACTGCAA TTGAGAAACC CACTGGAGAT 
GAACAGTCTT CAGGGTGTTT AGAAAACCAG CTACCTGCCT TTCTGGAAGA ACTTTGCCAT 
GAGAAAGAAA TTTTGGAGAA GTACGGACAT TCAGACTGCT GCAGCCAAAG TGAAGAGGGA 
AGACATAACT GTTTTCTTGC ACACAAAAAG CCCACTCCAG CATCGATCCC ACTTTTCCAA 
GTTCCAGAAC CTGTCACAAG CTGTGAAGCA TATGAAGAAG ACAGGGAGAC ATTCATGAAC 
AAATTCATTT ATGAGATAGC AAGAAGGCAT CCCTTCCTGT ATGCACCTAC AATTCTTCTT 
TGGGCTGCTC GCTATGACAA AATAATTCCA TCTTGCTGCA AAGCTGAAAA TGCAGTTGAA 
TGCTTCCAAA CAAAGGCAGC AACAGTTACA AAAGAATTAA GAGAAAGCAG CTTGTTAAAT 
CAACATGCAT GTGCAGTAAT GAAAAATTTT GGGACCCGAA CTTTCCAAGC CATAACTGTT 
ACTAAACTGA GTCAGAAGTT TACCAAAGTT AATTTTACTG AAATCCAGAA ACTAGTCCTG 
GATGTGGCCC ATGTACATGA GCACTGTTGC AGAGGAGATG TGCTGGATTG TCTGCAGGAT 
GGGGAAAAAA TCATGTCCTA CATATGTTCT CAACAAGACA CTCTGTCAAA CAAAATAACA 
GAATGCTGCA AACTGACCAC GCTGGAACGT GGTCAATGTA TAATTCATGC AGAAAATGAT 
GAAAAACCTG AAGGTCTATC TCCAAATCTA AACAGGTTTT TAGGAGATAG AGATTTTAAC 
CAATTTTCTT CAGGGGAAAA AAATATCTTC TTGGCAAGTT TTGTTCATGA ATATTCAAGA 
AGACATCCTC AGCTTGCTGT CTCAGTAATT CTAAGAGTTG CTAAAGGATA CCAGGAGTTA 
TTGGAGAAGT GTTTCCAGAC TGAAAACCCT CTTGAATGCC AAGATAAAGG AGAAGAAGAA 
TTACAGAAAT ACATCCAGGA GAGCCAAGCA TTGGCAAAGC GAAGCTGCGG CCTCTTCCAG 
AAACTAGGAG AATATTACTT ACAAAATGCG TTTCTCGTTG CTTACACAAA GAAAGCCCCC 
CAGCTGACCT CGTCGGAGCT GATGGCCATC ACCAGAAAAA TGGCAGCCAC AGCAGCCACT 
TGTTGCCAAC TCAGTGAGGA CAAACTATTG GCCTGTGGCG AGGGAGCGGC TGACATTATT 
ATCGGACACT TATGTATCAG ACATGAAATG ACTCCAGTAA ACCCTGGTGT TGGCCAGTGC 
TGCACTTCTT CATATGCCAA CAGGAGGCCA TGCTTCAGCA GCTTGGTGGT GGATGAAACA 
TATGTCCCTC CTGCATTCTC TGATGACAAG TTCATTTTCC ATAAGGATCT GTGCCAAGCT 
CAGGGTGTAG CGCTGCAAAC AATGAAGCAA GAGTTTCTCA TTAACCTTGT GAAGCAAAAG 
CCACAAATAA CAGAGGAACA ACTTGAGGCT GTCATTGCAG ATTTCTCAGG CCTGTTGGAG 
AAATGCTGCC AAGGCCAGGA ACAGGAAGTC TGCTTTGCTG AAGAGGGACA AAAACTGATT 
TCAAAAACTC GTGCTGCTTT GGGAGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001125.1
CDS	43..1872
Translation	MKWVESIFLIFLLNFTESRTLHRNEYGIASILDSYQCTAEISLADLATIFFAQFVQEATYKEVSKMVKDALTAIEKPTGDEQSSGCLENQLPAFLEELCHEKEILEKYGHSDCCSQSEEGRHNCFLAHKKPTPASIPLFQVPEPVTSCEAYEEDRETFMNKFIYEIARRHPFLYAPTILLWAARYDKIIPSCCKAENAVECFQTKAATVTKELRESSLLNQHACAVMKNFGTRTFQAITVTKLSQKFTKVNFTEIQKLVLDVAHVHEHCCRGDVLDCLQDGEKIMSYICSQQDTLSNKITECCKLTTLERGQCIIHAENDEKPEGLSPNLNRFLGDRDFNQFSSGEKNIFLASFVHEYSRRHPQLAVSVILRVAKGYQELLEKCFQTENPLECQDKGEEELQKYIQESQALAKRSCGLFQKLGEYYLQNAFLVAYTKKAPQLTSSELMAITRKMAATAATCCQLSEDKLLACGEGAADIIIGHLCIRHEMTPVNPGVGQCCTSSYANRRPCFSSLVVDETYVPPAFSDDKFIFHKDLCQAQGVALQTMKQEFLINLVKQKPQITEEQLEAVIADFSGLLEKCCQGQEQEVCFAEEGQKLISKTRAALGV

Target ORF information:

RefSeq Version	NM_001134.3
Organism	Homo sapiens(human)
Definition	Homo sapiens alpha fetoprotein (AFP), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001134.3

ORF Insert Sequence:

ATGAAGTGGG TGGAATCAAT TTTTTTAATT TTCCTACTAA ATTTTACTGA ATCCAGAACA 
CTGCATAGAA ATGAATATGG AATAGCTTCC ATATTGGATT CTTACCAATG TACTGCAGAG 
ATAAGTTTAG CTGACCTGGC TACCATATTT TTTGCCCAGT TTGTTCAAGA AGCCACTTAC 
AAGGAAGTAA GCAAAATGGT GAAAGATGCA TTGACTGCAA TTGAGAAACC CACTGGAGAT 
GAACAGTCTT CAGGGTGTTT AGAAAACCAG CTACCTGCCT TTCTGGAAGA ACTTTGCCAT 
GAGAAAGAAA TTTTGGAGAA GTACGGACAT TCAGACTGCT GCAGCCAAAG TGAAGAGGGA 
AGACATAACT GTTTTCTTGC ACACAAAAAG CCCACTCCAG CATCGATCCC ACTTTTCCAA 
GTTCCAGAAC CTGTCACAAG CTGTGAAGCA TATGAAGAAG ACAGGGAGAC ATTCATGAAC 
AAATTCATTT ATGAGATAGC AAGAAGGCAT CCCTTCCTGT ATGCACCTAC AATTCTTCTT 
TGGGCTGCTC GCTATGACAA AATAATTCCA TCTTGCTGCA AAGCTGAAAA TGCAGTTGAA 
TGCTTCCAAA CAAAGGCAGC AACAGTTACA AAAGAATTAA GAGAAAGCAG CTTGTTAAAT 
CAACATGCAT GTGCAGTAAT GAAAAATTTT GGGACCCGAA CTTTCCAAGC CATAACTGTT 
ACTAAACTGA GTCAGAAGTT TACCAAAGTT AATTTTACTG AAATCCAGAA ACTAGTCCTG 
GATGTGGCCC ATGTACATGA GCACTGTTGC AGAGGAGATG TGCTGGATTG TCTGCAGGAT 
GGGGAAAAAA TCATGTCCTA CATATGTTCT CAACAAGACA CTCTGTCAAA CAAAATAACA 
GAATGCTGCA AACTGACCAC GCTGGAACGT GGTCAATGTA TAATTCATGC AGAAAATGAT 
GAAAAACCTG AAGGTCTATC TCCAAATCTA AACAGGTTTT TAGGAGATAG AGATTTTAAC 
CAATTTTCTT CAGGGGAAAA AAATATCTTC TTGGCAAGTT TTGTTCATGA ATATTCAAGA 
AGACATCCTC AGCTTGCTGT CTCAGTAATT CTAAGAGTTG CTAAAGGATA CCAGGAGTTA 
TTGGAGAAGT GTTTCCAGAC TGAAAACCCT CTTGAATGCC AAGATAAAGG AGAAGAAGAA 
TTACAGAAAT ACATCCAGGA GAGCCAAGCA TTGGCAAAGC GAAGCTGCGG CCTCTTCCAG 
AAACTAGGAG AATATTACTT ACAAAATGCG TTTCTCGTTG CTTACACAAA GAAAGCCCCC 
CAGCTGACCT CGTCGGAGCT GATGGCCATC ACCAGAAAAA TGGCAGCCAC AGCAGCCACT 
TGTTGCCAAC TCAGTGAGGA CAAACTATTG GCCTGTGGCG AGGGAGCGGC TGACATTATT 
ATCGGACACT TATGTATCAG ACATGAAATG ACTCCAGTAA ACCCTGGTGT TGGCCAGTGC 
TGCACTTCTT CATATGCCAA CAGGAGGCCA TGCTTCAGCA GCTTGGTGGT GGATGAAACA 
TATGTCCCTC CTGCATTCTC TGATGACAAG TTCATTTTCC ATAAGGATCT GTGCCAAGCT 
CAGGGTGTAG CGCTGCAAAC AATGAAGCAA GAGTTTCTCA TTAACCTTGT GAAGCAAAAG 
CCACAAATAA CAGAGGAACA ACTTGAGGCT GTCATTGCAG ATTTCTCAGG CCTGTTGGAG 
AAATGCTGCC AAGGCCAGGA ACAGGAAGTC TGCTTTGCTG AAGAGGGACA AAAACTGATT 
TCAAAAACTC GTGCTGCTTT GGGAGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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AFP cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

見積依頼

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電話

FAX

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