DLD cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	DLD
Entrez Gene ID	1738
Full Name	dihydrolipoamide dehydrogenase
Synonyms	DLDD,DLDH,E3,GCSL,LAD,PHE3
General protein information	Preferred Names dihydrolipoamide dehydrogenase Names dihydrolipoyl dehydrogenase, mitochondrial E3 component of pyruvate dehydrogenase complex, 2-oxo-glutarate complex, branched chain keto acid dehydrogenase complex diaphorase glycine cleavage system L protein glycine cleavage system protein L lipoamide dehydrogenase lipoamide reductase lipoyl dehydrogenase
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 7 Map Location: 7q31.1
Summary	This gene encodes a member of the class-I pyridine nucleotide-disulfide oxidoreductase family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. In homodimeric form, the encoded protein functions as a dehydrogenase and is found in several multi-enzyme complexes that regulate energy metabolism. However, as a monomer, this protein can function as a protease. Mutations in this gene have been identified in patients with E3-deficient maple syrup urine disease and lipoamide dehydrogenase deficiency. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014].
Disorder MIM:	238331
Disorder Html:	Dihydrolipoamide dehydrogenase deficiency, 246900 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001289751.1	NP_001276680.1	dihydrolipoyl dehydrogenase, mitochondrial isoform 3
NM_000108.5	NP_000099.2	dihydrolipoyl dehydrogenase, mitochondrial isoform 1 precursor
NM_001289752.1	NP_001276681.1	dihydrolipoyl dehydrogenase, mitochondrial isoform 4
NM_001289750.1	NP_001276679.1	dihydrolipoyl dehydrogenase, mitochondrial isoform 2
NM_000108.4	NP_000099.2	dihydrolipoyl dehydrogenase, mitochondrial isoform 1 precursor

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Dld	NP_955417.1
Caenorhabditis elegans (roundworm)	dld-1	NP_001255810.1
Neurospora crassa	NCU02407	XP_959535.2
Arabidopsis thaliana (thale cress)	mtLPD2	NP_566570.3
Homo sapiens (human)	DLD	NP_000099.2
	DLD	XP_001095232.2
Canis lupus familiaris (dog)	DLD	NP_001003294.1
Mus musculus (house mouse)	Dld	NP_031887.2
Danio rerio (zebrafish)	dldh	NP_958914.1
Magnaporthe oryzae (rice blast fungus)	MGG_17072	XP_003716343.1
Pan troglodytes (chimpanzee)	DLD	XP_001165051.2
Gallus gallus (chicken)	DLD	NP_001025898.1
Drosophila melanogaster (fruit fly)	CG7430	NP_649017.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011629	XP_320877.4
Kluyveromyces lactis	KLLA0D11154g	XP_453559.1
Eremothecium gossypii	AGOS_AFR512W	NP_986059.2
Oryza sativa (rice)	Os01g0328700	NP_001042918.1
Bos taurus (cattle)	DLD	NP_001193099.1
Saccharomyces cerevisiae (baker's yeast)	LPD1	NP_116635.1
Schizosaccharomyces pombe (fission yeast)	dld1	NP_593496.1
Arabidopsis thaliana (thale cress)	mtLPD1	NP_175237.1
Xenopus tropicalis (tropical clawed frog)	dld	XP_002933161.2

Gene Ontology
Bibliography

Related articles in PubMed

A Map of Human Mitochondrial Protein Interactions Linked to Neurodegeneration Reveals New Mechanisms of Redox Homeostasis and NF-?B Signaling.
Malty RH, Aoki H, Kumar A, Phanse S, Amin S, Zhang Q, Minic Z, Goebels F, Musso G, Wu Z, Abou-Tok H, Meyer M, Deineko V, Kassir S, Sidhu V, Jessulat M, Scott NE, Xiong X, Vlasblom J, Prasad B, Foster LJ, Alberio T, Garavaglia B, Yu H, Bader GD, Nakamura K, Parkinson J, Babu M
Cell systems5(6)564-577.e12(2017 Dec)

KAT2A coupled with the ?-KGDH complex acts as a histone H3 succinyltransferase.
Wang Y, Guo YR, Liu K, Yin Z, Liu R, Xia Y, Tan L, Yang P, Lee JH, Li XJ, Hawke D, Zheng Y, Qian X, Lyu J, He J, Xing D, Tao YJ, Lu Z
Nature552(7684)273-277(2017 Dec)

The involvement of coordinative interactions in the binding of dihydrolipoamide dehydrogenase to titanium dioxide-Localization of a putative binding site.
Dayan A, Babin G, Ganoth A, Kayouf NS, Nitoker Eliaz N, Mukkala S, Tsfadia Y, Fleminger G
Journal of molecular recognition : JMR30(8)(2017 Aug)

Architecture of the human interactome defines protein communities and disease networks.
Huttlin EL, Bruckner RJ, Paulo JA, Cannon JR, Ting L, Baltier K, Colby G, Gebreab F, Gygi MP, Parzen H, Szpyt J, Tam S, Zarraga G, Pontano-Vaites L, Swarup S, White AE, Schweppe DK, Rad R, Erickson BK, Obar RA, Guruharsha KG, Li K, Artavanis-Tsakonas S, Gygi SP, Harper JW
Nature545(7655)505-509(2017 05)

Hepatitis B Virus Core Promoter Double Mutations (A1762T, G1764A) Are Associated with Lower Levels of Serum Dihydrolipoyl Dehydrogenase.
Jiang ZH, Chen QY, Harrison TJ, Li GJ, Wang XY, Li H, Hu LP, Li KW, Yang QL, Tan C, Fang ZL
Intervirology59(1)1-7(2016)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

study found that individuals infected with HBV withwith basal core promoter (BCP) double mutations (A1762T, G1764A)have lower concentrations of serum DLD than those with the wild-type BCP
Title: Hepatitis B Virus Core Promoter Double Mutations (A1762T, G1764A) Are Associated with Lower Levels of Serum Dihydrolipoyl Dehydrogenase.

Mitochondrial dihydrolipoamide dehydrogenase is upregulated in response to the brain intermittent hypoxic preconditioning.
Title: Mitochondrial Dihydrolipoamide Dehydrogenase is Upregulated in Response to Intermittent Hypoxic Preconditioning.

IgA autoantibody against DLD could be a novel diagnostic marker for endometrial cancer.
Title: Proteomic identification of dihydrolipoamide dehydrogenase as a target of autoantibodies in patients with endometrial cancer.

This molecular dynamics study proposes the structural changes that may lead to the modulation in reactive oxygen species generation by pathogenic mutants of human dihydrolipoamide dehydrogenase.
Title: Molecular dynamics study of the structural basis of dysfunction and the modulation of reactive oxygen species generation by pathogenic mutants of human dihydrolipoamide dehydrogenase.

ATP consumption is demonstrated in respiration-impaired isolated and in situ neuronal somal mitochondria from transgenic mice that exhibit a 20-48% decrease in alpha-ketoglutarate dehydrogenase activity.
Title: The negative impact of ?-ketoglutarate dehydrogenase complex deficiency on matrix substrate-level phosphorylation.

The following DLD gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DLD cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu11376	NM_001289750.1 Latest version!	Homo sapiens dihydrolipoamide dehydrogenase (DLD), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
OHu22614	NM_000108.5 Latest version!	Homo sapiens dihydrolipoamide dehydrogenase (DLD), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu10329	NM_001289752.1 Latest version!	Homo sapiens dihydrolipoamide dehydrogenase (DLD), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OHu11971	NM_001289751.1 Latest version!	Homo sapiens dihydrolipoamide dehydrogenase (DLD), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
View Old Accession Versions >>
OHu22614	NM_000108.4	Homo sapiens dihydrolipoamide dehydrogenase (DLD), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DLD ( NM_001289750.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001276679.1 Homo sapiens dihydrolipoamide dehydrogenase (DLD), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu11376
Clone ID Related Accession (Same CDS sequence)	NM_001289750.1
Accession Version	NM_001289750.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1233bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-09
Organism	Homo sapiens(human)
Product	dihydrolipoyl dehydrogenase, mitochondrial isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC354209.1, AK295080.1 and AC005046.3. Transcript Variant: This variant (2) lacks part of the 5' coding region, and uses a downstream start codon, compared to variant 1. The encoded isoform (2) has a shorter N-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.165809.1, SRR1803616.138276.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2142363, SAMEA2145240 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta multifunctional gene product(s) :: PMID: 17404228 ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCCCATG GAAAAGATTT TGCATCTAGA GGAATTGAAA TGTCCGAAGT TCGCTTGAAT 
TTAGACAAGA TGATGGAGCA GAAGAGTACT GCAGTAAAAG CTTTAACAGG TGGAATTGCC 
CACTTATTCA AACAGAATAA GGTTGTTCAT GTCAATGGAT ATGGAAAGAT AACTGGCAAA 
AATCAAGTCA CTGCTACGAA AGCTGATGGC GGCACTCAGG TTATTGATAC AAAGAACATT 
CTTATAGCCA CGGGTTCAGA AGTTACTCCT TTTCCTGGAA TCACGATAGA TGAAGATACA 
ATAGTGTCAT CTACAGGTGC TTTATCTTTA AAAAAAGTTC CAGAAAAGAT GGTTGTTATT 
GGTGCAGGAG TAATAGGTGT AGAATTGGGT TCAGTTTGGC AAAGACTTGG TGCAGATGTG 
ACAGCAGTTG AATTTTTAGG TCATGTAGGT GGAGTTGGAA TTGATATGGA GATATCTAAA 
AACTTTCAAC GCATCCTTCA AAAACAGGGG TTTAAATTTA AATTGAATAC AAAGGTTACT 
GGTGCTACCA AGAAGTCAGA TGGAAAAATT GATGTTTCTA TTGAAGCTGC TTCTGGTGGT 
AAAGCTGAAG TTATCACTTG TGATGTACTC TTGGTTTGCA TTGGCCGACG ACCCTTTACT 
AAGAATTTGG GACTAGAAGA GCTGGGAATT GAACTAGATC CCAGAGGTAG AATTCCAGTC 
AATACCAGAT TTCAAACTAA AATTCCAAAT ATCTATGCCA TTGGTGATGT AGTTGCTGGT 
CCAATGCTGG CTCACAAAGC AGAGGATGAA GGCATTATCT GTGTTGAAGG AATGGCTGGT 
GGTGCTGTGC ACATTGACTA CAATTGTGTG CCATCAGTGA TTTACACACA CCCTGAAGTT 
GCTTGGGTTG GCAAATCAGA AGAGCAGTTG AAAGAAGAGG GTATTGAGTA CAAAGTTGGG 
AAATTCCCAT TTGCTGCTAA CAGCAGAGCT AAGACAAATG CTGACACAGA TGGCATGGTG 
AAGATCCTTG GGCAGAAATC GACAGACAGA GTACTGGGAG CACATATTCT TGGACCAGGT 
GCTGGAGAAA TGGTAAATGA AGCTGCTCTT GCTTTGGAAT ATGGAGCATC CTGTGAAGAT 
ATAGCTAGAG TCTGTCATGC ACATCCGACC TTATCAGAAG CTTTTAGAGA AGCAAATCTT 
GCTGCGTCAT TTGGCAAATC AATCAACTTT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001276679.1
CDS	293..1525
Translation	MAHGKDFASRGIEMSEVRLNLDKMMEQKSTAVKALTGGIAHLFKQNKVVHVNGYGKITGKNQVTATKADGGTQVIDTKNILIATGSEVTPFPGITIDEDTIVSSTGALSLKKVPEKMVVIGAGVIGVELGSVWQRLGADVTAVEFLGHVGGVGIDMEISKNFQRILQKQGFKFKLNTKVTGATKKSDGKIDVSIEAASGGKAEVITCDVLLVCIGRRPFTKNLGLEELGIELDPRGRIPVNTRFQTKIPNIYAIGDVVAGPMLAHKAEDEGIICVEGMAGGAVHIDYNCVPSVIYTHPEVAWVGKSEEQLKEEGIEYKVGKFPFAANSRAKTNADTDGMVKILGQKSTDRVLGAHILGPGAGEMVNEAALALEYGASCEDIARVCHAHPTLSEAFREANLAASFGKSINF

Target ORF information:

RefSeq Version	NM_001289750.1
Organism	Homo sapiens(human)
Definition	Homo sapiens dihydrolipoamide dehydrogenase (DLD), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001289750.1

ORF Insert Sequence:

ATGGCCCATG GAAAAGATTT TGCATCTAGA GGAATTGAAA TGTCCGAAGT TCGCTTGAAT 
TTAGACAAGA TGATGGAGCA GAAGAGTACT GCAGTAAAAG CTTTAACAGG TGGAATTGCC 
CACTTATTCA AACAGAATAA GGTTGTTCAT GTCAATGGAT ATGGAAAGAT AACTGGCAAA 
AATCAAGTCA CTGCTACGAA AGCTGATGGC GGCACTCAGG TTATTGATAC AAAGAACATT 
CTTATAGCCA CGGGTTCAGA AGTTACTCCT TTTCCTGGAA TCACGATAGA TGAAGATACA 
ATAGTGTCAT CTACAGGTGC TTTATCTTTA AAAAAAGTTC CAGAAAAGAT GGTTGTTATT 
GGTGCAGGAG TAATAGGTGT AGAATTGGGT TCAGTTTGGC AAAGACTTGG TGCAGATGTG 
ACAGCAGTTG AATTTTTAGG TCATGTAGGT GGAGTTGGAA TTGATATGGA GATATCTAAA 
AACTTTCAAC GCATCCTTCA AAAACAGGGG TTTAAATTTA AATTGAATAC AAAGGTTACT 
GGTGCTACCA AGAAGTCAGA TGGAAAAATT GATGTTTCTA TTGAAGCTGC TTCTGGTGGT 
AAAGCTGAAG TTATCACTTG TGATGTACTC TTGGTTTGCA TTGGCCGACG ACCCTTTACT 
AAGAATTTGG GACTAGAAGA GCTGGGAATT GAACTAGATC CCAGAGGTAG AATTCCAGTC 
AATACCAGAT TTCAAACTAA AATTCCAAAT ATCTATGCCA TTGGTGATGT AGTTGCTGGT 
CCAATGCTGG CTCACAAAGC AGAGGATGAA GGCATTATCT GTGTTGAAGG AATGGCTGGT 
GGTGCTGTGC ACATTGACTA CAATTGTGTG CCATCAGTGA TTTACACACA CCCTGAAGTT 
GCTTGGGTTG GCAAATCAGA AGAGCAGTTG AAAGAAGAGG GTATTGAGTA CAAAGTTGGG 
AAATTCCCAT TTGCTGCTAA CAGCAGAGCT AAGACAAATG CTGACACAGA TGGCATGGTG 
AAGATCCTTG GGCAGAAATC GACAGACAGA GTACTGGGAG CACATATTCT TGGACCAGGT 
GCTGGAGAAA TGGTAAATGA AGCTGCTCTT GCTTTGGAAT ATGGAGCATC CTGTGAAGAT 
ATAGCTAGAG TCTGTCATGC ACATCCGACC TTATCAGAAG CTTTTAGAGA AGCAAATCTT 
GCTGCGTCAT TTGGCAAATC AATCAACTTT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DLD ( NM_000108.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_000099.2 Homo sapiens dihydrolipoamide dehydrogenase (DLD), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu22614
Clone ID Related Accession (Same CDS sequence)	NM_000108.5 , NM_000108.4
Accession Version	NM_000108.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1530bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-09
Organism	Homo sapiens(human)
Product	dihydrolipoyl dehydrogenase, mitochondrial isoform 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK312346.1, BC018648.2 and AC005046.3. This sequence is a reference standard in the RefSeqGene project. On Nov 22, 2018 this sequence version replaced NM_000108.4. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163655.72997.1, SRR1660805.174470.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta MANE Ensembl match :: ENST00000205402.10/ ENSP00000205402.3 multifunctional gene product(s) :: PMID: 17404228 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGCAGAGCT GGAGTCGTGT GTACTGCTCC TTGGCCAAGA GAGGCCATTT CAATCGAATA 
TCTCATGGCC TACAGGGACT TTCTGCAGTG CCTCTGAGAA CTTACGCAGA TCAGCCGATT 
GATGCTGATG TAACAGTTAT AGGTTCTGGT CCTGGAGGAT ATGTTGCTGC TATTAAAGCT 
GCCCAGTTAG GCTTCAAGAC AGTCTGCATT GAGAAAAATG AAACACTTGG TGGAACATGC 
TTGAATGTTG GTTGTATTCC TTCTAAGGCT TTATTGAACA ACTCTCATTA TTACCATATG 
GCCCATGGAA AAGATTTTGC ATCTAGAGGA ATTGAAATGT CCGAAGTTCG CTTGAATTTA 
GACAAGATGA TGGAGCAGAA GAGTACTGCA GTAAAAGCTT TAACAGGTGG AATTGCCCAC 
TTATTCAAAC AGAATAAGGT TGTTCATGTC AATGGATATG GAAAGATAAC TGGCAAAAAT 
CAAGTCACTG CTACGAAAGC TGATGGCGGC ACTCAGGTTA TTGATACAAA GAACATTCTT 
ATAGCCACGG GTTCAGAAGT TACTCCTTTT CCTGGAATCA CGATAGATGA AGATACAATA 
GTGTCATCTA CAGGTGCTTT ATCTTTAAAA AAAGTTCCAG AAAAGATGGT TGTTATTGGT 
GCAGGAGTAA TAGGTGTAGA ATTGGGTTCA GTTTGGCAAA GACTTGGTGC AGATGTGACA 
GCAGTTGAAT TTTTAGGTCA TGTAGGTGGA GTTGGAATTG ATATGGAGAT ATCTAAAAAC 
TTTCAACGCA TCCTTCAAAA ACAGGGGTTT AAATTTAAAT TGAATACAAA GGTTACTGGT 
GCTACCAAGA AGTCAGATGG AAAAATTGAT GTTTCTATTG AAGCTGCTTC TGGTGGTAAA 
GCTGAAGTTA TCACTTGTGA TGTACTCTTG GTTTGCATTG GCCGACGACC CTTTACTAAG 
AATTTGGGAC TAGAAGAGCT GGGAATTGAA CTAGATCCCA GAGGTAGAAT TCCAGTCAAT 
ACCAGATTTC AAACTAAAAT TCCAAATATC TATGCCATTG GTGATGTAGT TGCTGGTCCA 
ATGCTGGCTC ACAAAGCAGA GGATGAAGGC ATTATCTGTG TTGAAGGAAT GGCTGGTGGT 
GCTGTGCACA TTGACTACAA TTGTGTGCCA TCAGTGATTT ACACACACCC TGAAGTTGCT 
TGGGTTGGCA AATCAGAAGA GCAGTTGAAA GAAGAGGGTA TTGAGTACAA AGTTGGGAAA 
TTCCCATTTG CTGCTAACAG CAGAGCTAAG ACAAATGCTG ACACAGATGG CATGGTGAAG 
ATCCTTGGGC AGAAATCGAC AGACAGAGTA CTGGGAGCAC ATATTCTTGG ACCAGGTGCT 
GGAGAAATGG TAAATGAAGC TGCTCTTGCT TTGGAATATG GAGCATCCTG TGAAGATATA 
GCTAGAGTCT GTCATGCACA TCCGACCTTA TCAGAAGCTT TTAGAGAAGC AAATCTTGCT 
GCGTCATTTG GCAAATCAAT CAACTTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000099.2
CDS	69..1598
Translation	MQSWSRVYCSLAKRGHFNRISHGLQGLSAVPLRTYADQPIDADVTVIGSGPGGYVAAIKAAQLGFKTVCIEKNETLGGTCLNVGCIPSKALLNNSHYYHMAHGKDFASRGIEMSEVRLNLDKMMEQKSTAVKALTGGIAHLFKQNKVVHVNGYGKITGKNQVTATKADGGTQVIDTKNILIATGSEVTPFPGITIDEDTIVSSTGALSLKKVPEKMVVIGAGVIGVELGSVWQRLGADVTAVEFLGHVGGVGIDMEISKNFQRILQKQGFKFKLNTKVTGATKKSDGKIDVSIEAASGGKAEVITCDVLLVCIGRRPFTKNLGLEELGIELDPRGRIPVNTRFQTKIPNIYAIGDVVAGPMLAHKAEDEGIICVEGMAGGAVHIDYNCVPSVIYTHPEVAWVGKSEEQLKEEGIEYKVGKFPFAANSRAKTNADTDGMVKILGQKSTDRVLGAHILGPGAGEMVNEAALALEYGASCEDIARVCHAHPTLSEAFREANLAASFGKSINF

Target ORF information:

RefSeq Version	NM_000108.5
Organism	Homo sapiens(human)
Definition	Homo sapiens dihydrolipoamide dehydrogenase (DLD), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000108.5

ORF Insert Sequence:

ATGCAGAGCT GGAGTCGTGT GTACTGCTCC TTGGCCAAGA GAGGCCATTT CAATCGAATA 
TCTCATGGCC TACAGGGACT TTCTGCAGTG CCTCTGAGAA CTTACGCAGA TCAGCCGATT 
GATGCTGATG TAACAGTTAT AGGTTCTGGT CCTGGAGGAT ATGTTGCTGC TATTAAAGCT 
GCCCAGTTAG GCTTCAAGAC AGTCTGCATT GAGAAAAATG AAACACTTGG TGGAACATGC 
TTGAATGTTG GTTGTATTCC TTCTAAGGCT TTATTGAACA ACTCTCATTA TTACCATATG 
GCCCATGGAA AAGATTTTGC ATCTAGAGGA ATTGAAATGT CCGAAGTTCG CTTGAATTTA 
GACAAGATGA TGGAGCAGAA GAGTACTGCA GTAAAAGCTT TAACAGGTGG AATTGCCCAC 
TTATTCAAAC AGAATAAGGT TGTTCATGTC AATGGATATG GAAAGATAAC TGGCAAAAAT 
CAAGTCACTG CTACGAAAGC TGATGGCGGC ACTCAGGTTA TTGATACAAA GAACATTCTT 
ATAGCCACGG GTTCAGAAGT TACTCCTTTT CCTGGAATCA CGATAGATGA AGATACAATA 
GTGTCATCTA CAGGTGCTTT ATCTTTAAAA AAAGTTCCAG AAAAGATGGT TGTTATTGGT 
GCAGGAGTAA TAGGTGTAGA ATTGGGTTCA GTTTGGCAAA GACTTGGTGC AGATGTGACA 
GCAGTTGAAT TTTTAGGTCA TGTAGGTGGA GTTGGAATTG ATATGGAGAT ATCTAAAAAC 
TTTCAACGCA TCCTTCAAAA ACAGGGGTTT AAATTTAAAT TGAATACAAA GGTTACTGGT 
GCTACCAAGA AGTCAGATGG AAAAATTGAT GTTTCTATTG AAGCTGCTTC TGGTGGTAAA 
GCTGAAGTTA TCACTTGTGA TGTACTCTTG GTTTGCATTG GCCGACGACC CTTTACTAAG 
AATTTGGGAC TAGAAGAGCT GGGAATTGAA CTAGATCCCA GAGGTAGAAT TCCAGTCAAT 
ACCAGATTTC AAACTAAAAT TCCAAATATC TATGCCATTG GTGATGTAGT TGCTGGTCCA 
ATGCTGGCTC ACAAAGCAGA GGATGAAGGC ATTATCTGTG TTGAAGGAAT GGCTGGTGGT 
GCTGTGCACA TTGACTACAA TTGTGTGCCA TCAGTGATTT ACACACACCC TGAAGTTGCT 
TGGGTTGGCA AATCAGAAGA GCAGTTGAAA GAAGAGGGTA TTGAGTACAA AGTTGGGAAA 
TTCCCATTTG CTGCTAACAG CAGAGCTAAG ACAAATGCTG ACACAGATGG CATGGTGAAG 
ATCCTTGGGC AGAAATCGAC AGACAGAGTA CTGGGAGCAC ATATTCTTGG ACCAGGTGCT 
GGAGAAATGG TAAATGAAGC TGCTCTTGCT TTGGAATATG GAGCATCCTG TGAAGATATA 
GCTAGAGTCT GTCATGCACA TCCGACCTTA TCAGAAGCTT TTAGAGAAGC AAATCTTGCT 
GCGTCATTTG GCAAATCAAT CAACTTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DLD ( NM_000108.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_000099.2 Homo sapiens dihydrolipoamide dehydrogenase (DLD), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu22614
Clone ID Related Accession (Same CDS sequence)	NM_000108.5 , NM_000108.4
Accession Version	NM_000108.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1530bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-27
Organism	Homo sapiens(human)
Product	dihydrolipoyl dehydrogenase, mitochondrial isoform 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC354209.1, AK312346.1, BC018648.2 and AC005046.3. This sequence is a reference standard in the RefSeqGene project. On Jan 28, 2014 this sequence version replaced NM_000108.3. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.174470.1, SRR1660809.173450.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta multifunctional gene product(s) :: PMID: 17404228 ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGCAGAGCT GGAGTCGTGT GTACTGCTCC TTGGCCAAGA GAGGCCATTT CAATCGAATA 
TCTCATGGCC TACAGGGACT TTCTGCAGTG CCTCTGAGAA CTTACGCAGA TCAGCCGATT 
GATGCTGATG TAACAGTTAT AGGTTCTGGT CCTGGAGGAT ATGTTGCTGC TATTAAAGCT 
GCCCAGTTAG GCTTCAAGAC AGTCTGCATT GAGAAAAATG AAACACTTGG TGGAACATGC 
TTGAATGTTG GTTGTATTCC TTCTAAGGCT TTATTGAACA ACTCTCATTA TTACCATATG 
GCCCATGGAA AAGATTTTGC ATCTAGAGGA ATTGAAATGT CCGAAGTTCG CTTGAATTTA 
GACAAGATGA TGGAGCAGAA GAGTACTGCA GTAAAAGCTT TAACAGGTGG AATTGCCCAC 
TTATTCAAAC AGAATAAGGT TGTTCATGTC AATGGATATG GAAAGATAAC TGGCAAAAAT 
CAAGTCACTG CTACGAAAGC TGATGGCGGC ACTCAGGTTA TTGATACAAA GAACATTCTT 
ATAGCCACGG GTTCAGAAGT TACTCCTTTT CCTGGAATCA CGATAGATGA AGATACAATA 
GTGTCATCTA CAGGTGCTTT ATCTTTAAAA AAAGTTCCAG AAAAGATGGT TGTTATTGGT 
GCAGGAGTAA TAGGTGTAGA ATTGGGTTCA GTTTGGCAAA GACTTGGTGC AGATGTGACA 
GCAGTTGAAT TTTTAGGTCA TGTAGGTGGA GTTGGAATTG ATATGGAGAT ATCTAAAAAC 
TTTCAACGCA TCCTTCAAAA ACAGGGGTTT AAATTTAAAT TGAATACAAA GGTTACTGGT 
GCTACCAAGA AGTCAGATGG AAAAATTGAT GTTTCTATTG AAGCTGCTTC TGGTGGTAAA 
GCTGAAGTTA TCACTTGTGA TGTACTCTTG GTTTGCATTG GCCGACGACC CTTTACTAAG 
AATTTGGGAC TAGAAGAGCT GGGAATTGAA CTAGATCCCA GAGGTAGAAT TCCAGTCAAT 
ACCAGATTTC AAACTAAAAT TCCAAATATC TATGCCATTG GTGATGTAGT TGCTGGTCCA 
ATGCTGGCTC ACAAAGCAGA GGATGAAGGC ATTATCTGTG TTGAAGGAAT GGCTGGTGGT 
GCTGTGCACA TTGACTACAA TTGTGTGCCA TCAGTGATTT ACACACACCC TGAAGTTGCT 
TGGGTTGGCA AATCAGAAGA GCAGTTGAAA GAAGAGGGTA TTGAGTACAA AGTTGGGAAA 
TTCCCATTTG CTGCTAACAG CAGAGCTAAG ACAAATGCTG ACACAGATGG CATGGTGAAG 
ATCCTTGGGC AGAAATCGAC AGACAGAGTA CTGGGAGCAC ATATTCTTGG ACCAGGTGCT 
GGAGAAATGG TAAATGAAGC TGCTCTTGCT TTGGAATATG GAGCATCCTG TGAAGATATA 
GCTAGAGTCT GTCATGCACA TCCGACCTTA TCAGAAGCTT TTAGAGAAGC AAATCTTGCT 
GCGTCATTTG GCAAATCAAT CAACTTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000099.2
CDS	145..1674
Translation	MQSWSRVYCSLAKRGHFNRISHGLQGLSAVPLRTYADQPIDADVTVIGSGPGGYVAAIKAAQLGFKTVCIEKNETLGGTCLNVGCIPSKALLNNSHYYHMAHGKDFASRGIEMSEVRLNLDKMMEQKSTAVKALTGGIAHLFKQNKVVHVNGYGKITGKNQVTATKADGGTQVIDTKNILIATGSEVTPFPGITIDEDTIVSSTGALSLKKVPEKMVVIGAGVIGVELGSVWQRLGADVTAVEFLGHVGGVGIDMEISKNFQRILQKQGFKFKLNTKVTGATKKSDGKIDVSIEAASGGKAEVITCDVLLVCIGRRPFTKNLGLEELGIELDPRGRIPVNTRFQTKIPNIYAIGDVVAGPMLAHKAEDEGIICVEGMAGGAVHIDYNCVPSVIYTHPEVAWVGKSEEQLKEEGIEYKVGKFPFAANSRAKTNADTDGMVKILGQKSTDRVLGAHILGPGAGEMVNEAALALEYGASCEDIARVCHAHPTLSEAFREANLAASFGKSINF

Target ORF information:

RefSeq Version	NM_000108.4
Organism	Homo sapiens(human)
Definition	Homo sapiens dihydrolipoamide dehydrogenase (DLD), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000108.4

ORF Insert Sequence:

ATGCAGAGCT GGAGTCGTGT GTACTGCTCC TTGGCCAAGA GAGGCCATTT CAATCGAATA 
TCTCATGGCC TACAGGGACT TTCTGCAGTG CCTCTGAGAA CTTACGCAGA TCAGCCGATT 
GATGCTGATG TAACAGTTAT AGGTTCTGGT CCTGGAGGAT ATGTTGCTGC TATTAAAGCT 
GCCCAGTTAG GCTTCAAGAC AGTCTGCATT GAGAAAAATG AAACACTTGG TGGAACATGC 
TTGAATGTTG GTTGTATTCC TTCTAAGGCT TTATTGAACA ACTCTCATTA TTACCATATG 
GCCCATGGAA AAGATTTTGC ATCTAGAGGA ATTGAAATGT CCGAAGTTCG CTTGAATTTA 
GACAAGATGA TGGAGCAGAA GAGTACTGCA GTAAAAGCTT TAACAGGTGG AATTGCCCAC 
TTATTCAAAC AGAATAAGGT TGTTCATGTC AATGGATATG GAAAGATAAC TGGCAAAAAT 
CAAGTCACTG CTACGAAAGC TGATGGCGGC ACTCAGGTTA TTGATACAAA GAACATTCTT 
ATAGCCACGG GTTCAGAAGT TACTCCTTTT CCTGGAATCA CGATAGATGA AGATACAATA 
GTGTCATCTA CAGGTGCTTT ATCTTTAAAA AAAGTTCCAG AAAAGATGGT TGTTATTGGT 
GCAGGAGTAA TAGGTGTAGA ATTGGGTTCA GTTTGGCAAA GACTTGGTGC AGATGTGACA 
GCAGTTGAAT TTTTAGGTCA TGTAGGTGGA GTTGGAATTG ATATGGAGAT ATCTAAAAAC 
TTTCAACGCA TCCTTCAAAA ACAGGGGTTT AAATTTAAAT TGAATACAAA GGTTACTGGT 
GCTACCAAGA AGTCAGATGG AAAAATTGAT GTTTCTATTG AAGCTGCTTC TGGTGGTAAA 
GCTGAAGTTA TCACTTGTGA TGTACTCTTG GTTTGCATTG GCCGACGACC CTTTACTAAG 
AATTTGGGAC TAGAAGAGCT GGGAATTGAA CTAGATCCCA GAGGTAGAAT TCCAGTCAAT 
ACCAGATTTC AAACTAAAAT TCCAAATATC TATGCCATTG GTGATGTAGT TGCTGGTCCA 
ATGCTGGCTC ACAAAGCAGA GGATGAAGGC ATTATCTGTG TTGAAGGAAT GGCTGGTGGT 
GCTGTGCACA TTGACTACAA TTGTGTGCCA TCAGTGATTT ACACACACCC TGAAGTTGCT 
TGGGTTGGCA AATCAGAAGA GCAGTTGAAA GAAGAGGGTA TTGAGTACAA AGTTGGGAAA 
TTCCCATTTG CTGCTAACAG CAGAGCTAAG ACAAATGCTG ACACAGATGG CATGGTGAAG 
ATCCTTGGGC AGAAATCGAC AGACAGAGTA CTGGGAGCAC ATATTCTTGG ACCAGGTGCT 
GGAGAAATGG TAAATGAAGC TGCTCTTGCT TTGGAATATG GAGCATCCTG TGAAGATATA 
GCTAGAGTCT GTCATGCACA TCCGACCTTA TCAGAAGCTT TTAGAGAAGC AAATCTTGCT 
GCGTCATTTG GCAAATCAAT CAACTTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DLD ( NM_001289752.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001276681.1 Homo sapiens dihydrolipoamide dehydrogenase (DLD), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OHu10329
Clone ID Related Accession (Same CDS sequence)	NM_001289752.1
Accession Version	NM_001289752.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1386bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-09
Organism	Homo sapiens(human)
Product	dihydrolipoyl dehydrogenase, mitochondrial isoform 4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC354209.1, AK312346.1, BC018648.2 and AC005046.3. Transcript Variant: This variant (4) lacks an in-frame exon in the central coding region, compared to variant 1. The encoded isoform (4) is shorter, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK300077.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2157437, SAMN02400308 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta multifunctional gene product(s) :: PMID: 17404228 ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGCAGAGCT GGAGTCGTGT GTACTGCTCC TTGGCCAAGA GAGGCCATTT CAATCGAATA 
TCTCATGGCC TACAGGGACT TTCTGCAGTG CCTCTGAGAA CTTACGCAGA TCAGCCGATT 
GATGCTGATG TAACAGTTAT AGGTTCTGGT CCTGGAGGAT ATGTTGCTGC TATTAAAGCT 
GCCCAGTTAG GCTTCAAGAC AGTCTGCATT GAGAAAAATG AAACACTTGG TGGAACATGC 
TTGAATGTTG GTTGTATTCC TTCTAAGGCT TTATTGAACA ACTCTCATTA TTACCATATG 
GCCCATGGAA AAGATTTTGC ATCTAGAGGA ATTGAAATGT CCGAAGTTCG CTTGAATTTA 
GACAAGATGA TGGAGCAGAA GAGTACTGCA GTAAAAGCTT TAACAGGTGG AATTGCCCAC 
TTATTCAAAC AGAATAAGAT AGATGAAGAT ACAATAGTGT CATCTACAGG TGCTTTATCT 
TTAAAAAAAG TTCCAGAAAA GATGGTTGTT ATTGGTGCAG GAGTAATAGG TGTAGAATTG 
GGTTCAGTTT GGCAAAGACT TGGTGCAGAT GTGACAGCAG TTGAATTTTT AGGTCATGTA 
GGTGGAGTTG GAATTGATAT GGAGATATCT AAAAACTTTC AACGCATCCT TCAAAAACAG 
GGGTTTAAAT TTAAATTGAA TACAAAGGTT ACTGGTGCTA CCAAGAAGTC AGATGGAAAA 
ATTGATGTTT CTATTGAAGC TGCTTCTGGT GGTAAAGCTG AAGTTATCAC TTGTGATGTA 
CTCTTGGTTT GCATTGGCCG ACGACCCTTT ACTAAGAATT TGGGACTAGA AGAGCTGGGA 
ATTGAACTAG ATCCCAGAGG TAGAATTCCA GTCAATACCA GATTTCAAAC TAAAATTCCA 
AATATCTATG CCATTGGTGA TGTAGTTGCT GGTCCAATGC TGGCTCACAA AGCAGAGGAT 
GAAGGCATTA TCTGTGTTGA AGGAATGGCT GGTGGTGCTG TGCACATTGA CTACAATTGT 
GTGCCATCAG TGATTTACAC ACACCCTGAA GTTGCTTGGG TTGGCAAATC AGAAGAGCAG 
TTGAAAGAAG AGGGTATTGA GTACAAAGTT GGGAAATTCC CATTTGCTGC TAACAGCAGA 
GCTAAGACAA ATGCTGACAC AGATGGCATG GTGAAGATCC TTGGGCAGAA ATCGACAGAC 
AGAGTACTGG GAGCACATAT TCTTGGACCA GGTGCTGGAG AAATGGTAAA TGAAGCTGCT 
CTTGCTTTGG AATATGGAGC ATCCTGTGAA GATATAGCTA GAGTCTGTCA TGCACATCCG 
ACCTTATCAG AAGCTTTTAG AGAAGCAAAT CTTGCTGCGT CATTTGGCAA ATCAATCAAC 
TTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001276681.1
CDS	145..1530
Translation	MQSWSRVYCSLAKRGHFNRISHGLQGLSAVPLRTYADQPIDADVTVIGSGPGGYVAAIKAAQLGFKTVCIEKNETLGGTCLNVGCIPSKALLNNSHYYHMAHGKDFASRGIEMSEVRLNLDKMMEQKSTAVKALTGGIAHLFKQNKIDEDTIVSSTGALSLKKVPEKMVVIGAGVIGVELGSVWQRLGADVTAVEFLGHVGGVGIDMEISKNFQRILQKQGFKFKLNTKVTGATKKSDGKIDVSIEAASGGKAEVITCDVLLVCIGRRPFTKNLGLEELGIELDPRGRIPVNTRFQTKIPNIYAIGDVVAGPMLAHKAEDEGIICVEGMAGGAVHIDYNCVPSVIYTHPEVAWVGKSEEQLKEEGIEYKVGKFPFAANSRAKTNADTDGMVKILGQKSTDRVLGAHILGPGAGEMVNEAALALEYGASCEDIARVCHAHPTLSEAFREANLAASFGKSINF

Target ORF information:

RefSeq Version	NM_001289752.1
Organism	Homo sapiens(human)
Definition	Homo sapiens dihydrolipoamide dehydrogenase (DLD), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001289752.1

ORF Insert Sequence:

ATGCAGAGCT GGAGTCGTGT GTACTGCTCC TTGGCCAAGA GAGGCCATTT CAATCGAATA 
TCTCATGGCC TACAGGGACT TTCTGCAGTG CCTCTGAGAA CTTACGCAGA TCAGCCGATT 
GATGCTGATG TAACAGTTAT AGGTTCTGGT CCTGGAGGAT ATGTTGCTGC TATTAAAGCT 
GCCCAGTTAG GCTTCAAGAC AGTCTGCATT GAGAAAAATG AAACACTTGG TGGAACATGC 
TTGAATGTTG GTTGTATTCC TTCTAAGGCT TTATTGAACA ACTCTCATTA TTACCATATG 
GCCCATGGAA AAGATTTTGC ATCTAGAGGA ATTGAAATGT CCGAAGTTCG CTTGAATTTA 
GACAAGATGA TGGAGCAGAA GAGTACTGCA GTAAAAGCTT TAACAGGTGG AATTGCCCAC 
TTATTCAAAC AGAATAAGAT AGATGAAGAT ACAATAGTGT CATCTACAGG TGCTTTATCT 
TTAAAAAAAG TTCCAGAAAA GATGGTTGTT ATTGGTGCAG GAGTAATAGG TGTAGAATTG 
GGTTCAGTTT GGCAAAGACT TGGTGCAGAT GTGACAGCAG TTGAATTTTT AGGTCATGTA 
GGTGGAGTTG GAATTGATAT GGAGATATCT AAAAACTTTC AACGCATCCT TCAAAAACAG 
GGGTTTAAAT TTAAATTGAA TACAAAGGTT ACTGGTGCTA CCAAGAAGTC AGATGGAAAA 
ATTGATGTTT CTATTGAAGC TGCTTCTGGT GGTAAAGCTG AAGTTATCAC TTGTGATGTA 
CTCTTGGTTT GCATTGGCCG ACGACCCTTT ACTAAGAATT TGGGACTAGA AGAGCTGGGA 
ATTGAACTAG ATCCCAGAGG TAGAATTCCA GTCAATACCA GATTTCAAAC TAAAATTCCA 
AATATCTATG CCATTGGTGA TGTAGTTGCT GGTCCAATGC TGGCTCACAA AGCAGAGGAT 
GAAGGCATTA TCTGTGTTGA AGGAATGGCT GGTGGTGCTG TGCACATTGA CTACAATTGT 
GTGCCATCAG TGATTTACAC ACACCCTGAA GTTGCTTGGG TTGGCAAATC AGAAGAGCAG 
TTGAAAGAAG AGGGTATTGA GTACAAAGTT GGGAAATTCC CATTTGCTGC TAACAGCAGA 
GCTAAGACAA ATGCTGACAC AGATGGCATG GTGAAGATCC TTGGGCAGAA ATCGACAGAC 
AGAGTACTGG GAGCACATAT TCTTGGACCA GGTGCTGGAG AAATGGTAAA TGAAGCTGCT 
CTTGCTTTGG AATATGGAGC ATCCTGTGAA GATATAGCTA GAGTCTGTCA TGCACATCCG 
ACCTTATCAG AAGCTTTTAG AGAAGCAAAT CTTGCTGCGT CATTTGGCAA ATCAATCAAC 
TTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DLD ( NM_001289751.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001276680.1 Homo sapiens dihydrolipoamide dehydrogenase (DLD), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu11971
Clone ID Related Accession (Same CDS sequence)	NM_001289751.1
Accession Version	NM_001289751.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1461bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-10
Organism	Homo sapiens(human)
Product	dihydrolipoyl dehydrogenase, mitochondrial isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC354209.1, AK312346.1, BC018648.2 and AC005046.3. Transcript Variant: This variant (3) lacks an in-frame exon in the 5' coding region, compared to variant 1. The encoded isoform (3) is shorter, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK297508.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta multifunctional gene product(s) :: PMID: 17404228 ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGCAGAGCT GGAGTCGTGT GTACTGCTCC TTGGCCAAGA GAGGCCATTT CAATCGAATA 
TCTCATGGCC TACAGGGACT TTCTGCAGTG CCTCTGAGAA CTTACGCAGA TCAGCCGATT 
GATGCTGATG TAACAGTTAT AGGTTCTGGT CCTGGAGGAT ATGTTGCTGC TATTAAAGCT 
GCCCAGTTAG GCTTCAAGGC TTTATTGAAC AACTCTCATT ATTACCATAT GGCCCATGGA 
AAAGATTTTG CATCTAGAGG AATTGAAATG TCCGAAGTTC GCTTGAATTT AGACAAGATG 
ATGGAGCAGA AGAGTACTGC AGTAAAAGCT TTAACAGGTG GAATTGCCCA CTTATTCAAA 
CAGAATAAGG TTGTTCATGT CAATGGATAT GGAAAGATAA CTGGCAAAAA TCAAGTCACT 
GCTACGAAAG CTGATGGCGG CACTCAGGTT ATTGATACAA AGAACATTCT TATAGCCACG 
GGTTCAGAAG TTACTCCTTT TCCTGGAATC ACGATAGATG AAGATACAAT AGTGTCATCT 
ACAGGTGCTT TATCTTTAAA AAAAGTTCCA GAAAAGATGG TTGTTATTGG TGCAGGAGTA 
ATAGGTGTAG AATTGGGTTC AGTTTGGCAA AGACTTGGTG CAGATGTGAC AGCAGTTGAA 
TTTTTAGGTC ATGTAGGTGG AGTTGGAATT GATATGGAGA TATCTAAAAA CTTTCAACGC 
ATCCTTCAAA AACAGGGGTT TAAATTTAAA TTGAATACAA AGGTTACTGG TGCTACCAAG 
AAGTCAGATG GAAAAATTGA TGTTTCTATT GAAGCTGCTT CTGGTGGTAA AGCTGAAGTT 
ATCACTTGTG ATGTACTCTT GGTTTGCATT GGCCGACGAC CCTTTACTAA GAATTTGGGA 
CTAGAAGAGC TGGGAATTGA ACTAGATCCC AGAGGTAGAA TTCCAGTCAA TACCAGATTT 
CAAACTAAAA TTCCAAATAT CTATGCCATT GGTGATGTAG TTGCTGGTCC AATGCTGGCT 
CACAAAGCAG AGGATGAAGG CATTATCTGT GTTGAAGGAA TGGCTGGTGG TGCTGTGCAC 
ATTGACTACA ATTGTGTGCC ATCAGTGATT TACACACACC CTGAAGTTGC TTGGGTTGGC 
AAATCAGAAG AGCAGTTGAA AGAAGAGGGT ATTGAGTACA AAGTTGGGAA ATTCCCATTT 
GCTGCTAACA GCAGAGCTAA GACAAATGCT GACACAGATG GCATGGTGAA GATCCTTGGG 
CAGAAATCGA CAGACAGAGT ACTGGGAGCA CATATTCTTG GACCAGGTGC TGGAGAAATG 
GTAAATGAAG CTGCTCTTGC TTTGGAATAT GGAGCATCCT GTGAAGATAT AGCTAGAGTC 
TGTCATGCAC ATCCGACCTT ATCAGAAGCT TTTAGAGAAG CAAATCTTGC TGCGTCATTT 
GGCAAATCAA TCAACTTTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001276680.1
CDS	145..1605
Translation	MQSWSRVYCSLAKRGHFNRISHGLQGLSAVPLRTYADQPIDADVTVIGSGPGGYVAAIKAAQLGFKALLNNSHYYHMAHGKDFASRGIEMSEVRLNLDKMMEQKSTAVKALTGGIAHLFKQNKVVHVNGYGKITGKNQVTATKADGGTQVIDTKNILIATGSEVTPFPGITIDEDTIVSSTGALSLKKVPEKMVVIGAGVIGVELGSVWQRLGADVTAVEFLGHVGGVGIDMEISKNFQRILQKQGFKFKLNTKVTGATKKSDGKIDVSIEAASGGKAEVITCDVLLVCIGRRPFTKNLGLEELGIELDPRGRIPVNTRFQTKIPNIYAIGDVVAGPMLAHKAEDEGIICVEGMAGGAVHIDYNCVPSVIYTHPEVAWVGKSEEQLKEEGIEYKVGKFPFAANSRAKTNADTDGMVKILGQKSTDRVLGAHILGPGAGEMVNEAALALEYGASCEDIARVCHAHPTLSEAFREANLAASFGKSINF

Target ORF information:

RefSeq Version	NM_001289751.1
Organism	Homo sapiens(human)
Definition	Homo sapiens dihydrolipoamide dehydrogenase (DLD), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001289751.1

ORF Insert Sequence:

ATGCAGAGCT GGAGTCGTGT GTACTGCTCC TTGGCCAAGA GAGGCCATTT CAATCGAATA 
TCTCATGGCC TACAGGGACT TTCTGCAGTG CCTCTGAGAA CTTACGCAGA TCAGCCGATT 
GATGCTGATG TAACAGTTAT AGGTTCTGGT CCTGGAGGAT ATGTTGCTGC TATTAAAGCT 
GCCCAGTTAG GCTTCAAGGC TTTATTGAAC AACTCTCATT ATTACCATAT GGCCCATGGA 
AAAGATTTTG CATCTAGAGG AATTGAAATG TCCGAAGTTC GCTTGAATTT AGACAAGATG 
ATGGAGCAGA AGAGTACTGC AGTAAAAGCT TTAACAGGTG GAATTGCCCA CTTATTCAAA 
CAGAATAAGG TTGTTCATGT CAATGGATAT GGAAAGATAA CTGGCAAAAA TCAAGTCACT 
GCTACGAAAG CTGATGGCGG CACTCAGGTT ATTGATACAA AGAACATTCT TATAGCCACG 
GGTTCAGAAG TTACTCCTTT TCCTGGAATC ACGATAGATG AAGATACAAT AGTGTCATCT 
ACAGGTGCTT TATCTTTAAA AAAAGTTCCA GAAAAGATGG TTGTTATTGG TGCAGGAGTA 
ATAGGTGTAG AATTGGGTTC AGTTTGGCAA AGACTTGGTG CAGATGTGAC AGCAGTTGAA 
TTTTTAGGTC ATGTAGGTGG AGTTGGAATT GATATGGAGA TATCTAAAAA CTTTCAACGC 
ATCCTTCAAA AACAGGGGTT TAAATTTAAA TTGAATACAA AGGTTACTGG TGCTACCAAG 
AAGTCAGATG GAAAAATTGA TGTTTCTATT GAAGCTGCTT CTGGTGGTAA AGCTGAAGTT 
ATCACTTGTG ATGTACTCTT GGTTTGCATT GGCCGACGAC CCTTTACTAA GAATTTGGGA 
CTAGAAGAGC TGGGAATTGA ACTAGATCCC AGAGGTAGAA TTCCAGTCAA TACCAGATTT 
CAAACTAAAA TTCCAAATAT CTATGCCATT GGTGATGTAG TTGCTGGTCC AATGCTGGCT 
CACAAAGCAG AGGATGAAGG CATTATCTGT GTTGAAGGAA TGGCTGGTGG TGCTGTGCAC 
ATTGACTACA ATTGTGTGCC ATCAGTGATT TACACACACC CTGAAGTTGC TTGGGTTGGC 
AAATCAGAAG AGCAGTTGAA AGAAGAGGGT ATTGAGTACA AAGTTGGGAA ATTCCCATTT 
GCTGCTAACA GCAGAGCTAA GACAAATGCT GACACAGATG GCATGGTGAA GATCCTTGGG 
CAGAAATCGA CAGACAGAGT ACTGGGAGCA CATATTCTTG GACCAGGTGC TGGAGAAATG 
GTAAATGAAG CTGCTCTTGC TTTGGAATAT GGAGCATCCT GTGAAGATAT AGCTAGAGTC 
TGTCATGCAC ATCCGACCTT ATCAGAAGCT TTTAGAGAAG CAAATCTTGC TGCGTCATTT 
GGCAAATCAA TCAACTTTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A Map of Human Mitochondrial Protein Interactions Linked to Neurodegeneration Reveals New Mechanisms of Redox Homeostasis and NF-?B Signaling.
Cell systems5(6)564-577.e12(2017 Dec)
Malty RH,Aoki H,Kumar A,Phanse S,Amin S,Zhang Q,Minic Z,Goebels F,Musso G,Wu Z,Abou-Tok H,Meyer M,Deineko V,Kassir S,Sidhu V,Jessulat M,Scott NE,Xiong X,Vlasblom J,Prasad B,Foster LJ,Alberio T,Garavaglia B,Yu H,Bader GD,Nakamura K,Parkinson J,Babu M

KAT2A coupled with the ?-KGDH complex acts as a histone H3 succinyltransferase.
Nature552(7684)273-277(2017 Dec)
Wang Y,Guo YR,Liu K,Yin Z,Liu R,Xia Y,Tan L,Yang P,Lee JH,Li XJ,Hawke D,Zheng Y,Qian X,Lyu J,He J,Xing D,Tao YJ,Lu Z

The involvement of coordinative interactions in the binding of dihydrolipoamide dehydrogenase to titanium dioxide-Localization of a putative binding site.
Journal of molecular recognition : JMR30(8)(2017 Aug)
Dayan A,Babin G,Ganoth A,Kayouf NS,Nitoker Eliaz N,Mukkala S,Tsfadia Y,Fleminger G

Architecture of the human interactome defines protein communities and disease networks.
Nature545(7655)505-509(2017 05)
Huttlin EL,Bruckner RJ,Paulo JA,Cannon JR,Ting L,Baltier K,Colby G,Gebreab F,Gygi MP,Parzen H,Szpyt J,Tam S,Zarraga G,Pontano-Vaites L,Swarup S,White AE,Schweppe DK,Rad R,Erickson BK,Obar RA,Guruharsha KG,Li K,Artavanis-Tsakonas S,Gygi SP,Harper JW

Hepatitis B Virus Core Promoter Double Mutations (A1762T, G1764A) Are Associated with Lower Levels of Serum Dihydrolipoyl Dehydrogenase.
Intervirology59(1)1-7(2016)
Jiang ZH,Chen QY,Harrison TJ,Li GJ,Wang XY,Li H,Hu LP,Li KW,Yang QL,Tan C,Fang ZL

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DLD cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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