DIAPH1 cDNA ORF clone, Homo sapiens(human)

The following DIAPH1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DIAPH1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu26409 NM_005219.5
Latest version!
Homo sapiens diaphanous related formin 1 (DIAPH1), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$274.50-$384.30
$549.00
OHu105637 XM_024454384.1
Latest version!
Homo sapiens diaphanous related formin 1 (DIAPH1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OHu105638 XM_024454385.1
Latest version!
Homo sapiens diaphanous related formin 1 (DIAPH1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OHu105639 XM_024454386.1
Latest version!
Homo sapiens diaphanous related formin 1 (DIAPH1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OHu105640 XM_024454387.1
Latest version!
Homo sapiens diaphanous related formin 1 (DIAPH1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OHu26133 NM_001079812.3
Latest version!
Homo sapiens diaphanous related formin 1 (DIAPH1), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $384.30
$549.00
OHu39824 NM_001314007.2
Latest version!
Homo sapiens diaphanous related formin 1 (DIAPH1), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $643.30
$919.00
OHu57238 XM_011537572.2
Latest version!
Homo sapiens diaphanous related formin 1 (DIAPH1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $643.30
$919.00
OHu57239 XM_011537573.2
Latest version!
Homo sapiens diaphanous related formin 1 (DIAPH1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $643.30
$919.00
View Old Accession Versions >>
OHu26409 NM_005219.4 Homo sapiens diaphanous related formin 1 (DIAPH1), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$274.50-$384.30
$549.00
OHu39824 NM_001314007.1 Homo sapiens diaphanous related formin 1 (DIAPH1), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $643.30
$919.00
OHu26133 NM_001079812.2 Homo sapiens diaphanous related formin 1 (DIAPH1), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $384.30
$549.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu26409
    Clone ID Related Accession (Same CDS sequence) NM_005219.4 , NM_005219.5
    Accession Version NM_005219.4 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3819bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-12-05
    Organism Homo sapiens(human)
    Product protein diaphanous homolog 1 isoform 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA593734.1, AB209482.1, AY363395.1 and AY007129.1. This sequence is a reference standard in the RefSeqGene project. On May 24, 2008 this sequence version replaced NM_005219.3. Transcript Variant: This variant (1) encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB209482.1, BC143413.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGAGCCGC CCGGCGGGAG CCTGGGGCCC GGCCGCGGGA CCCGGGACAA GAAGAAGGGC 
    CGGAGCCCAG ATGAGCTGCC CTCGGCGGGC GGCGACGGCG GCAAATCTAA GAAATTTACT
    CTGAAGCGGC TCATGGCAGA TGAGCTGGAG AGATTTACCA GCATGAGAAT TAAGAAGGAG
    AAGGAAAAGC CCAATTCTGC TCATAGAAAT TCTTCTGCAT CATATGGGGA TGATCCCACA
    GCACAGTCAT TGCAAGATGT TTCAGATGAA CAAGTGCTGG TTCTCTTTGA ACAGATGCTG
    CTGGATATGA ACCTGAATGA GGAGAAACAG CAACCTTTGA GGGAGAAGGA CATCATCATC
    AAGAGGGAGA TGGTGTCCCA ATACTTGTAC ACCTCCAAGG CTGGCATGAG CCAGAAGGAG
    AGCTCTAAGT CTGCCATGAT GTATATTCAG GAGTTGAGGT CAGGCTTGCG GGATATGCCT
    CTGCTCAGCT GCCTGGAGTC CCTTCGTGTG TCTCTCAACA ACAACCCTGT CAGTTGGGTG
    CAAACATTTG GTGCTGAAGG CTTGGCCTCC TTATTGGACA TTCTTAAACG ACTTCATGAT
    GAGAAAGAAG AGACTGCTGG GAGTTACGAT AGCCGGAACA AGCATGAGAT CATTCGCTGC
    TTGAAAGCTT TTATGAACAA CAAGTTTGGA ATCAAGACCA TGTTGGAGAC AGAAGAAGGA
    ATCCTACTGC TGGTCAGAGC CATGGATCCT GCTGTTCCCA ACATGATGAT TGATGCAGCT
    AAGCTGCTTT CTGCTCTTTG TATTCTACCG CAGCCAGAGG ACATGAATGA AAGGGTTTTG
    GAGGCAATGA CAGAAAGAGC TGAGATGGAT GAAGTGGAAC GTTTCCAGCC GCTGCTGGAT
    GGATTAAAAA GTGGAACCAC TATTGCACTG AAGGTTGGAT GCCTACAGCT GATCAATGCT
    CTCATCACAC CAGCGGAGGA ACTTGACTTC CGAGTTCACA TCAGAAGTGA ACTGATGCGT
    TTGGGGCTAC ATCAGGTGTT GCAGGACCTT CGAGAGATTG AAAATGAAGA TATGAGAGTG
    CAACTAAATG TGTTTGATGA ACAAGGGGAA GAGGATTCCT ATGACCTGAA GGGACGGCTG
    GATGACATTC GCATGGAGAT GGATGACTTT AATGAAGTCT TTCAGATTCT CTTAAACACA
    GTGAAGGATT CAAAGGCAGA GCCACACTTC CTTTCCATCC TGCAGCACTT ACTCTTGGTC
    CGAAATGACT ATGAGGCCAG ACCTCAGTAC TATAAGTTGA TTGAAGAATG TATTTCCCAG
    ATAGTTCTGC ACAAGAACGG GGCTGATCCT GACTTCAAGT GCCGGCACCT CCAGATTGAG
    ATTGAGGGAT TAATTGATCA AATGATTGAT AAGACAAAGG TGGAGAAATC TGAAGCCAAA
    GCTGCAGAGC TGGAAAAGAA GTTGGACTCA GAGTTAACAG CCCGACATGA GCTACAGGTG
    GAAATGAAAA AGATGGAAAG TGACTTTGAG CAGAAGCTTC AAGATCTTCA GGGAGAAAAA
    GATGCACTGC ATTCTGAAAA GCAGCAAATT GCCACAGAGA AACAGGACCT GGAAGCAGAG
    GTGTCCCAGC TCACAGGAGA GGTTGCCAAG CTGACAAAGG AACTGGAAGA TGCCAAGAAA
    GAAATGGCTT CCCTCTCTGC GGCAGCTATT ACTGTACCTC CTTCTGTTCC TAGTCGTGCT
    CCTGTTCCCC CTGCCCCTCC TTTACCTGGT GACTCTGGCA CTATTATTCC ACCACCACCT
    GCTCCTGGGG ATAGTACCAC TCCTCCTCCT CCTCCTCCTC CTCCTCCTCC TCCACCTCCT
    TTGCCTGGGG GTGTTTGCAT CTCCTCACCC CCTTCTTTAC CTGGAGGTAC TGCTATCTCT
    CCACCCCCTC CTTTGTCTGG GGATGCTACC ATCCCTCCAC CCCCTCCTTT GCCTGAGGGT
    GTTGGCATCC CTTCACCCTC TTCTTTGCCT GGAGGTACTG CCATCCCCCC ACCTCCTCCT
    TTGCCTGGGA GTGCTAGAAT CCCCCCACCA CCACCTCCTT TGCCTGGGAG TGCTGGAATT
    CCCCCCCCAC CTCCTCCCTT GCCTGGAGAA GCAGGAATGC CACCTCCTCC TCCCCCTCTT
    CCTGGTGGTC CTGGAATCCC TCCACCTCCT CCATTTCCCG GAGGCCCTGG CATTCCTCCA
    CCTCCACCCG GAATGGGTAT GCCTCCACCT CCCCCATTTG GATTTGGAGT TCCTGCAGCC
    CCAGTTCTGC CATTTGGATT AACCCCCAAA AAGCTTTATA AGCCAGAGGT GCAGCTCCGG
    AGGCCAAACT GGTCCAAGCT TGTGGCTGAG GACCTCTCCC AGGACTGCTT CTGGACAAAG
    GTGAAGGAGG ACCGCTTTGA GAACAATGAA CTTTTCGCCA AACTTACCCT TACCTTCTCT
    GCCCAGACCA AGACTTCCAA AGCCAAGAAG GATCAAGAAG GTGGAGAAGA AAAGAAATCT
    GTGCAAAAGA AAAAAGTAAA AGAGTTAAAG GTGTTGGATT CAAAGACAGC CCAGAATCTC
    TCAATCTTTT TGGGTTCCTT CCGCATGCCC TATCAAGAGA TTAAGAATGT CATCCTGGAG
    GTGAATGAGG CTGTTCTGAC TGAGTCTATG ATCCAGAACC TCATTAAGCA AATGCCAGAG
    CCAGAGCAGT TAAAAATGCT TTCTGAACTG AAGGATGAAT ATGATGACCT GGCTGAGTCA
    GAGCAGTTTG GCGTGGTGAT GGGCACTGTG CCCCGACTGC GGCCTCGCCT CAATGCCATT
    CTCTTCAAGC TACAATTCAG CGAGCAAGTG GAGAATATCA AGCCAGAGAT TGTGTCTGTC
    ACTGCTGCAT GTGAGGAGTT ACGTAAGAGT GAGAGCTTTT CCAATCTCCT AGAGATTACC
    TTGCTTGTTG GAAATTACAT GAATGCTGGC TCCAGAAATG CTGGTGCTTT TGGCTTCAAT
    ATCAGCTTCC TCTGTAAGCT TCGAGACACC AAGTCCACAG ATCAGAAGAT GACGTTGTTA
    CACTTCTTGG CTGAGTTGTG TGAGAATGAC TATCCCGATG TCCTCAAGTT TCCAGACGAG
    CTTGCCCATG TGGAGAAAGC CAGCCGAGTT TCTGCTGAAA ACTTGCAAAA GAACCTAGAT
    CAGATGAAGA AACAAATTTC TGATGTGGAA CGTGATGTTC AGAATTTCCC AGCTGCCACA
    GATGAAAAAG ACAAGTTTGT TGAAAAAATG ACCAGCTTTG TGAAGGATGC ACAGGAACAG
    TATAACAAGC TGCGGATGAT GCATTCTAAC ATGGAGACCC TCTATAAGGA GCTGGGCGAG
    TACTTCCTCT TTGACCCCAA GAAGTTGTCT GTTGAAGAAT TTTTCATGGA TCTTCACAAT
    TTTCGGAATA TGTTTTTGCA AGCAGTCAAG GAGAACCAGA AGCGGCGGGA GACAGAAGAA
    AAGATGAGGC GAGCAAAACT AGCCAAGGAG AAGGCAGAGA AGGAGCGGCT AGAGAAGCAG
    CAGAAGAGAG AGCAACTCAT AGACATGAAT GCAGAGGGCG ATGAGACAGG TGTGATGGAC
    AGTCTTCTAG AAGCCCTGCA GTCAGGGGCA GCATTCCGAC GGAAGAGAGG GCCCCGTCAA
    GCCAACAGGA AGGCCGGGTG TGCAGTCACA TCTCTGCTAG CTTCGGAGCT GACCAAGGAT
    GATGCCATGG CTGCTGTTCC TGCCAAGGTG TCCAAGAACA GTGAGACATT CCCCACAATC
    CTTGAGGAAG CCAAGGAGTT GGTTGGCCGT GCAAGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_005210.3
    CDS142..3960
    Translation

    Target ORF information:

    RefSeq Version NM_005219.4
    Organism Homo sapiens(human)
    Definition Homo sapiens diaphanous related formin 1 (DIAPH1), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_005219.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGAGCCGC CCGGCGGGAG CCTGGGGCCC GGCCGCGGGA CCCGGGACAA GAAGAAGGGC 
    CGGAGCCCAG ATGAGCTGCC CTCGGCGGGC GGCGACGGCG GCAAATCTAA GAAATTTACT
    CTGAAGCGGC TCATGGCAGA TGAGCTGGAG AGATTTACCA GCATGAGAAT TAAGAAGGAG
    AAGGAAAAGC CCAATTCTGC TCATAGAAAT TCTTCTGCAT CATATGGGGA TGATCCCACA
    GCACAGTCAT TGCAAGATGT TTCAGATGAA CAAGTGCTGG TTCTCTTTGA ACAGATGCTG
    CTGGATATGA ACCTGAATGA GGAGAAACAG CAACCTTTGA GGGAGAAGGA CATCATCATC
    AAGAGGGAGA TGGTGTCCCA ATACTTGTAC ACCTCCAAGG CTGGCATGAG CCAGAAGGAG
    AGCTCTAAGT CTGCCATGAT GTATATTCAG GAGTTGAGGT CAGGCTTGCG GGATATGCCT
    CTGCTCAGCT GCCTGGAGTC CCTTCGTGTG TCTCTCAACA ACAACCCTGT CAGTTGGGTG
    CAAACATTTG GTGCTGAAGG CTTGGCCTCC TTATTGGACA TTCTTAAACG ACTTCATGAT
    GAGAAAGAAG AGACTGCTGG GAGTTACGAT AGCCGGAACA AGCATGAGAT CATTCGCTGC
    TTGAAAGCTT TTATGAACAA CAAGTTTGGA ATCAAGACCA TGTTGGAGAC AGAAGAAGGA
    ATCCTACTGC TGGTCAGAGC CATGGATCCT GCTGTTCCCA ACATGATGAT TGATGCAGCT
    AAGCTGCTTT CTGCTCTTTG TATTCTACCG CAGCCAGAGG ACATGAATGA AAGGGTTTTG
    GAGGCAATGA CAGAAAGAGC TGAGATGGAT GAAGTGGAAC GTTTCCAGCC GCTGCTGGAT
    GGATTAAAAA GTGGAACCAC TATTGCACTG AAGGTTGGAT GCCTACAGCT GATCAATGCT
    CTCATCACAC CAGCGGAGGA ACTTGACTTC CGAGTTCACA TCAGAAGTGA ACTGATGCGT
    TTGGGGCTAC ATCAGGTGTT GCAGGACCTT CGAGAGATTG AAAATGAAGA TATGAGAGTG
    CAACTAAATG TGTTTGATGA ACAAGGGGAA GAGGATTCCT ATGACCTGAA GGGACGGCTG
    GATGACATTC GCATGGAGAT GGATGACTTT AATGAAGTCT TTCAGATTCT CTTAAACACA
    GTGAAGGATT CAAAGGCAGA GCCACACTTC CTTTCCATCC TGCAGCACTT ACTCTTGGTC
    CGAAATGACT ATGAGGCCAG ACCTCAGTAC TATAAGTTGA TTGAAGAATG TATTTCCCAG
    ATAGTTCTGC ACAAGAACGG GGCTGATCCT GACTTCAAGT GCCGGCACCT CCAGATTGAG
    ATTGAGGGAT TAATTGATCA AATGATTGAT AAGACAAAGG TGGAGAAATC TGAAGCCAAA
    GCTGCAGAGC TGGAAAAGAA GTTGGACTCA GAGTTAACAG CCCGACATGA GCTACAGGTG
    GAAATGAAAA AGATGGAAAG TGACTTTGAG CAGAAGCTTC AAGATCTTCA GGGAGAAAAA
    GATGCACTGC ATTCTGAAAA GCAGCAAATT GCCACAGAGA AACAGGACCT GGAAGCAGAG
    GTGTCCCAGC TCACAGGAGA GGTTGCCAAG CTGACAAAGG AACTGGAAGA TGCCAAGAAA
    GAAATGGCTT CCCTCTCTGC GGCAGCTATT ACTGTACCTC CTTCTGTTCC TAGTCGTGCT
    CCTGTTCCCC CTGCCCCTCC TTTACCTGGT GACTCTGGCA CTATTATTCC ACCACCACCT
    GCTCCTGGGG ATAGTACCAC TCCTCCTCCT CCTCCTCCTC CTCCTCCTCC TCCACCTCCT
    TTGCCTGGGG GTGTTTGCAT CTCCTCACCC CCTTCTTTAC CTGGAGGTAC TGCTATCTCT
    CCACCCCCTC CTTTGTCTGG GGATGCTACC ATCCCTCCAC CCCCTCCTTT GCCTGAGGGT
    GTTGGCATCC CTTCACCCTC TTCTTTGCCT GGAGGTACTG CCATCCCCCC ACCTCCTCCT
    TTGCCTGGGA GTGCTAGAAT CCCCCCACCA CCACCTCCTT TGCCTGGGAG TGCTGGAATT
    CCCCCCCCAC CTCCTCCCTT GCCTGGAGAA GCAGGAATGC CACCTCCTCC TCCCCCTCTT
    CCTGGTGGTC CTGGAATCCC TCCACCTCCT CCATTTCCCG GAGGCCCTGG CATTCCTCCA
    CCTCCACCCG GAATGGGTAT GCCTCCACCT CCCCCATTTG GATTTGGAGT TCCTGCAGCC
    CCAGTTCTGC CATTTGGATT AACCCCCAAA AAGCTTTATA AGCCAGAGGT GCAGCTCCGG
    AGGCCAAACT GGTCCAAGCT TGTGGCTGAG GACCTCTCCC AGGACTGCTT CTGGACAAAG
    GTGAAGGAGG ACCGCTTTGA GAACAATGAA CTTTTCGCCA AACTTACCCT TACCTTCTCT
    GCCCAGACCA AGACTTCCAA AGCCAAGAAG GATCAAGAAG GTGGAGAAGA AAAGAAATCT
    GTGCAAAAGA AAAAAGTAAA AGAGTTAAAG GTGTTGGATT CAAAGACAGC CCAGAATCTC
    TCAATCTTTT TGGGTTCCTT CCGCATGCCC TATCAAGAGA TTAAGAATGT CATCCTGGAG
    GTGAATGAGG CTGTTCTGAC TGAGTCTATG ATCCAGAACC TCATTAAGCA AATGCCAGAG
    CCAGAGCAGT TAAAAATGCT TTCTGAACTG AAGGATGAAT ATGATGACCT GGCTGAGTCA
    GAGCAGTTTG GCGTGGTGAT GGGCACTGTG CCCCGACTGC GGCCTCGCCT CAATGCCATT
    CTCTTCAAGC TACAATTCAG CGAGCAAGTG GAGAATATCA AGCCAGAGAT TGTGTCTGTC
    ACTGCTGCAT GTGAGGAGTT ACGTAAGAGT GAGAGCTTTT CCAATCTCCT AGAGATTACC
    TTGCTTGTTG GAAATTACAT GAATGCTGGC TCCAGAAATG CTGGTGCTTT TGGCTTCAAT
    ATCAGCTTCC TCTGTAAGCT TCGAGACACC AAGTCCACAG ATCAGAAGAT GACGTTGTTA
    CACTTCTTGG CTGAGTTGTG TGAGAATGAC TATCCCGATG TCCTCAAGTT TCCAGACGAG
    CTTGCCCATG TGGAGAAAGC CAGCCGAGTT TCTGCTGAAA ACTTGCAAAA GAACCTAGAT
    CAGATGAAGA AACAAATTTC TGATGTGGAA CGTGATGTTC AGAATTTCCC AGCTGCCACA
    GATGAAAAAG ACAAGTTTGT TGAAAAAATG ACCAGCTTTG TGAAGGATGC ACAGGAACAG
    TATAACAAGC TGCGGATGAT GCATTCTAAC ATGGAGACCC TCTATAAGGA GCTGGGCGAG
    TACTTCCTCT TTGACCCCAA GAAGTTGTCT GTTGAAGAAT TTTTCATGGA TCTTCACAAT
    TTTCGGAATA TGTTTTTGCA AGCAGTCAAG GAGAACCAGA AGCGGCGGGA GACAGAAGAA
    AAGATGAGGC GAGCAAAACT AGCCAAGGAG AAGGCAGAGA AGGAGCGGCT AGAGAAGCAG
    CAGAAGAGAG AGCAACTCAT AGACATGAAT GCAGAGGGCG ATGAGACAGG TGTGATGGAC
    AGTCTTCTAG AAGCCCTGCA GTCAGGGGCA GCATTCCGAC GGAAGAGAGG GCCCCGTCAA
    GCCAACAGGA AGGCCGGGTG TGCAGTCACA TCTCTGCTAG CTTCGGAGCT GACCAAGGAT
    GATGCCATGG CTGCTGTTCC TGCCAAGGTG TCCAAGAACA GTGAGACATT CCCCACAATC
    CTTGAGGAAG CCAAGGAGTT GGTTGGCCGT GCAAGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu26409
    Clone ID Related Accession (Same CDS sequence) NM_005219.4 , NM_005219.5
    Accession Version NM_005219.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3819bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-09
    Organism Homo sapiens(human)
    Product protein diaphanous homolog 1 isoform 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA593734.1, AB209482.1, AY363395.1 and AY007129.1. This sequence is a reference standard in the RefSeqGene project. On Nov 22, 2018 this sequence version replaced NM_005219.4. Transcript Variant: This variant (1) encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB209482.1, BC143413.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000389054.8/ ENSP00000373706.4 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGAGCCGC CCGGCGGGAG CCTGGGGCCC GGCCGCGGGA CCCGGGACAA GAAGAAGGGC 
    CGGAGCCCAG ATGAGCTGCC CTCGGCGGGC GGCGACGGCG GCAAATCTAA GAAATTTACT
    CTGAAGCGGC TCATGGCAGA TGAGCTGGAG AGATTTACCA GCATGAGAAT TAAGAAGGAG
    AAGGAAAAGC CCAATTCTGC TCATAGAAAT TCTTCTGCAT CATATGGGGA TGATCCCACA
    GCACAGTCAT TGCAAGATGT TTCAGATGAA CAAGTGCTGG TTCTCTTTGA ACAGATGCTG
    CTGGATATGA ACCTGAATGA GGAGAAACAG CAACCTTTGA GGGAGAAGGA CATCATCATC
    AAGAGGGAGA TGGTGTCCCA ATACTTGTAC ACCTCCAAGG CTGGCATGAG CCAGAAGGAG
    AGCTCTAAGT CTGCCATGAT GTATATTCAG GAGTTGAGGT CAGGCTTGCG GGATATGCCT
    CTGCTCAGCT GCCTGGAGTC CCTTCGTGTG TCTCTCAACA ACAACCCTGT CAGTTGGGTG
    CAAACATTTG GTGCTGAAGG CTTGGCCTCC TTATTGGACA TTCTTAAACG ACTTCATGAT
    GAGAAAGAAG AGACTGCTGG GAGTTACGAT AGCCGGAACA AGCATGAGAT CATTCGCTGC
    TTGAAAGCTT TTATGAACAA CAAGTTTGGA ATCAAGACCA TGTTGGAGAC AGAAGAAGGA
    ATCCTACTGC TGGTCAGAGC CATGGATCCT GCTGTTCCCA ACATGATGAT TGATGCAGCT
    AAGCTGCTTT CTGCTCTTTG TATTCTACCG CAGCCAGAGG ACATGAATGA AAGGGTTTTG
    GAGGCAATGA CAGAAAGAGC TGAGATGGAT GAAGTGGAAC GTTTCCAGCC GCTGCTGGAT
    GGATTAAAAA GTGGAACCAC TATTGCACTG AAGGTTGGAT GCCTACAGCT GATCAATGCT
    CTCATCACAC CAGCGGAGGA ACTTGACTTC CGAGTTCACA TCAGAAGTGA ACTGATGCGT
    TTGGGGCTAC ATCAGGTGTT GCAGGACCTT CGAGAGATTG AAAATGAAGA TATGAGAGTG
    CAACTAAATG TGTTTGATGA ACAAGGGGAA GAGGATTCCT ATGACCTGAA GGGACGGCTG
    GATGACATTC GCATGGAGAT GGATGACTTT AATGAAGTCT TTCAGATTCT CTTAAACACA
    GTGAAGGATT CAAAGGCAGA GCCACACTTC CTTTCCATCC TGCAGCACTT ACTCTTGGTC
    CGAAATGACT ATGAGGCCAG ACCTCAGTAC TATAAGTTGA TTGAAGAATG TATTTCCCAG
    ATAGTTCTGC ACAAGAACGG GGCTGATCCT GACTTCAAGT GCCGGCACCT CCAGATTGAG
    ATTGAGGGAT TAATTGATCA AATGATTGAT AAGACAAAGG TGGAGAAATC TGAAGCCAAA
    GCTGCAGAGC TGGAAAAGAA GTTGGACTCA GAGTTAACAG CCCGACATGA GCTACAGGTG
    GAAATGAAAA AGATGGAAAG TGACTTTGAG CAGAAGCTTC AAGATCTTCA GGGAGAAAAA
    GATGCACTGC ATTCTGAAAA GCAGCAAATT GCCACAGAGA AACAGGACCT GGAAGCAGAG
    GTGTCCCAGC TCACAGGAGA GGTTGCCAAG CTGACAAAGG AACTGGAAGA TGCCAAGAAA
    GAAATGGCTT CCCTCTCTGC GGCAGCTATT ACTGTACCTC CTTCTGTTCC TAGTCGTGCT
    CCTGTTCCCC CTGCCCCTCC TTTACCTGGT GACTCTGGCA CTATTATTCC ACCACCACCT
    GCTCCTGGGG ATAGTACCAC TCCTCCTCCT CCTCCTCCTC CTCCTCCTCC TCCACCTCCT
    TTGCCTGGGG GTGTTTGCAT CTCCTCACCC CCTTCTTTAC CTGGAGGTAC TGCTATCTCT
    CCACCCCCTC CTTTGTCTGG GGATGCTACC ATCCCTCCAC CCCCTCCTTT GCCTGAGGGT
    GTTGGCATCC CTTCACCCTC TTCTTTGCCT GGAGGTACTG CCATCCCCCC ACCTCCTCCT
    TTGCCTGGGA GTGCTAGAAT CCCCCCACCA CCACCTCCTT TGCCTGGGAG TGCTGGAATT
    CCCCCCCCAC CTCCTCCCTT GCCTGGAGAA GCAGGAATGC CACCTCCTCC TCCCCCTCTT
    CCTGGTGGTC CTGGAATCCC TCCACCTCCT CCATTTCCCG GAGGCCCTGG CATTCCTCCA
    CCTCCACCCG GAATGGGTAT GCCTCCACCT CCCCCATTTG GATTTGGAGT TCCTGCAGCC
    CCAGTTCTGC CATTTGGATT AACCCCCAAA AAGCTTTATA AGCCAGAGGT GCAGCTCCGG
    AGGCCAAACT GGTCCAAGCT TGTGGCTGAG GACCTCTCCC AGGACTGCTT CTGGACAAAG
    GTGAAGGAGG ACCGCTTTGA GAACAATGAA CTTTTCGCCA AACTTACCCT TACCTTCTCT
    GCCCAGACCA AGACTTCCAA AGCCAAGAAG GATCAAGAAG GTGGAGAAGA AAAGAAATCT
    GTGCAAAAGA AAAAAGTAAA AGAGTTAAAG GTGTTGGATT CAAAGACAGC CCAGAATCTC
    TCAATCTTTT TGGGTTCCTT CCGCATGCCC TATCAAGAGA TTAAGAATGT CATCCTGGAG
    GTGAATGAGG CTGTTCTGAC TGAGTCTATG ATCCAGAACC TCATTAAGCA AATGCCAGAG
    CCAGAGCAGT TAAAAATGCT TTCTGAACTG AAGGATGAAT ATGATGACCT GGCTGAGTCA
    GAGCAGTTTG GCGTGGTGAT GGGCACTGTG CCCCGACTGC GGCCTCGCCT CAATGCCATT
    CTCTTCAAGC TACAATTCAG CGAGCAAGTG GAGAATATCA AGCCAGAGAT TGTGTCTGTC
    ACTGCTGCAT GTGAGGAGTT ACGTAAGAGT GAGAGCTTTT CCAATCTCCT AGAGATTACC
    TTGCTTGTTG GAAATTACAT GAATGCTGGC TCCAGAAATG CTGGTGCTTT TGGCTTCAAT
    ATCAGCTTCC TCTGTAAGCT TCGAGACACC AAGTCCACAG ATCAGAAGAT GACGTTGTTA
    CACTTCTTGG CTGAGTTGTG TGAGAATGAC TATCCCGATG TCCTCAAGTT TCCAGACGAG
    CTTGCCCATG TGGAGAAAGC CAGCCGAGTT TCTGCTGAAA ACTTGCAAAA GAACCTAGAT
    CAGATGAAGA AACAAATTTC TGATGTGGAA CGTGATGTTC AGAATTTCCC AGCTGCCACA
    GATGAAAAAG ACAAGTTTGT TGAAAAAATG ACCAGCTTTG TGAAGGATGC ACAGGAACAG
    TATAACAAGC TGCGGATGAT GCATTCTAAC ATGGAGACCC TCTATAAGGA GCTGGGCGAG
    TACTTCCTCT TTGACCCCAA GAAGTTGTCT GTTGAAGAAT TTTTCATGGA TCTTCACAAT
    TTTCGGAATA TGTTTTTGCA AGCAGTCAAG GAGAACCAGA AGCGGCGGGA GACAGAAGAA
    AAGATGAGGC GAGCAAAACT AGCCAAGGAG AAGGCAGAGA AGGAGCGGCT AGAGAAGCAG
    CAGAAGAGAG AGCAACTCAT AGACATGAAT GCAGAGGGCG ATGAGACAGG TGTGATGGAC
    AGTCTTCTAG AAGCCCTGCA GTCAGGGGCA GCATTCCGAC GGAAGAGAGG GCCCCGTCAA
    GCCAACAGGA AGGCCGGGTG TGCAGTCACA TCTCTGCTAG CTTCGGAGCT GACCAAGGAT
    GATGCCATGG CTGCTGTTCC TGCCAAGGTG TCCAAGAACA GTGAGACATT CCCCACAATC
    CTTGAGGAAG CCAAGGAGTT GGTTGGCCGT GCAAGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_005210.3
    CDS87..3905
    Translation

    Target ORF information:

    RefSeq Version NM_005219.5
    Organism Homo sapiens(human)
    Definition Homo sapiens diaphanous related formin 1 (DIAPH1), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_005219.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGAGCCGC CCGGCGGGAG CCTGGGGCCC GGCCGCGGGA CCCGGGACAA GAAGAAGGGC 
    CGGAGCCCAG ATGAGCTGCC CTCGGCGGGC GGCGACGGCG GCAAATCTAA GAAATTTACT
    CTGAAGCGGC TCATGGCAGA TGAGCTGGAG AGATTTACCA GCATGAGAAT TAAGAAGGAG
    AAGGAAAAGC CCAATTCTGC TCATAGAAAT TCTTCTGCAT CATATGGGGA TGATCCCACA
    GCACAGTCAT TGCAAGATGT TTCAGATGAA CAAGTGCTGG TTCTCTTTGA ACAGATGCTG
    CTGGATATGA ACCTGAATGA GGAGAAACAG CAACCTTTGA GGGAGAAGGA CATCATCATC
    AAGAGGGAGA TGGTGTCCCA ATACTTGTAC ACCTCCAAGG CTGGCATGAG CCAGAAGGAG
    AGCTCTAAGT CTGCCATGAT GTATATTCAG GAGTTGAGGT CAGGCTTGCG GGATATGCCT
    CTGCTCAGCT GCCTGGAGTC CCTTCGTGTG TCTCTCAACA ACAACCCTGT CAGTTGGGTG
    CAAACATTTG GTGCTGAAGG CTTGGCCTCC TTATTGGACA TTCTTAAACG ACTTCATGAT
    GAGAAAGAAG AGACTGCTGG GAGTTACGAT AGCCGGAACA AGCATGAGAT CATTCGCTGC
    TTGAAAGCTT TTATGAACAA CAAGTTTGGA ATCAAGACCA TGTTGGAGAC AGAAGAAGGA
    ATCCTACTGC TGGTCAGAGC CATGGATCCT GCTGTTCCCA ACATGATGAT TGATGCAGCT
    AAGCTGCTTT CTGCTCTTTG TATTCTACCG CAGCCAGAGG ACATGAATGA AAGGGTTTTG
    GAGGCAATGA CAGAAAGAGC TGAGATGGAT GAAGTGGAAC GTTTCCAGCC GCTGCTGGAT
    GGATTAAAAA GTGGAACCAC TATTGCACTG AAGGTTGGAT GCCTACAGCT GATCAATGCT
    CTCATCACAC CAGCGGAGGA ACTTGACTTC CGAGTTCACA TCAGAAGTGA ACTGATGCGT
    TTGGGGCTAC ATCAGGTGTT GCAGGACCTT CGAGAGATTG AAAATGAAGA TATGAGAGTG
    CAACTAAATG TGTTTGATGA ACAAGGGGAA GAGGATTCCT ATGACCTGAA GGGACGGCTG
    GATGACATTC GCATGGAGAT GGATGACTTT AATGAAGTCT TTCAGATTCT CTTAAACACA
    GTGAAGGATT CAAAGGCAGA GCCACACTTC CTTTCCATCC TGCAGCACTT ACTCTTGGTC
    CGAAATGACT ATGAGGCCAG ACCTCAGTAC TATAAGTTGA TTGAAGAATG TATTTCCCAG
    ATAGTTCTGC ACAAGAACGG GGCTGATCCT GACTTCAAGT GCCGGCACCT CCAGATTGAG
    ATTGAGGGAT TAATTGATCA AATGATTGAT AAGACAAAGG TGGAGAAATC TGAAGCCAAA
    GCTGCAGAGC TGGAAAAGAA GTTGGACTCA GAGTTAACAG CCCGACATGA GCTACAGGTG
    GAAATGAAAA AGATGGAAAG TGACTTTGAG CAGAAGCTTC AAGATCTTCA GGGAGAAAAA
    GATGCACTGC ATTCTGAAAA GCAGCAAATT GCCACAGAGA AACAGGACCT GGAAGCAGAG
    GTGTCCCAGC TCACAGGAGA GGTTGCCAAG CTGACAAAGG AACTGGAAGA TGCCAAGAAA
    GAAATGGCTT CCCTCTCTGC GGCAGCTATT ACTGTACCTC CTTCTGTTCC TAGTCGTGCT
    CCTGTTCCCC CTGCCCCTCC TTTACCTGGT GACTCTGGCA CTATTATTCC ACCACCACCT
    GCTCCTGGGG ATAGTACCAC TCCTCCTCCT CCTCCTCCTC CTCCTCCTCC TCCACCTCCT
    TTGCCTGGGG GTGTTTGCAT CTCCTCACCC CCTTCTTTAC CTGGAGGTAC TGCTATCTCT
    CCACCCCCTC CTTTGTCTGG GGATGCTACC ATCCCTCCAC CCCCTCCTTT GCCTGAGGGT
    GTTGGCATCC CTTCACCCTC TTCTTTGCCT GGAGGTACTG CCATCCCCCC ACCTCCTCCT
    TTGCCTGGGA GTGCTAGAAT CCCCCCACCA CCACCTCCTT TGCCTGGGAG TGCTGGAATT
    CCCCCCCCAC CTCCTCCCTT GCCTGGAGAA GCAGGAATGC CACCTCCTCC TCCCCCTCTT
    CCTGGTGGTC CTGGAATCCC TCCACCTCCT CCATTTCCCG GAGGCCCTGG CATTCCTCCA
    CCTCCACCCG GAATGGGTAT GCCTCCACCT CCCCCATTTG GATTTGGAGT TCCTGCAGCC
    CCAGTTCTGC CATTTGGATT AACCCCCAAA AAGCTTTATA AGCCAGAGGT GCAGCTCCGG
    AGGCCAAACT GGTCCAAGCT TGTGGCTGAG GACCTCTCCC AGGACTGCTT CTGGACAAAG
    GTGAAGGAGG ACCGCTTTGA GAACAATGAA CTTTTCGCCA AACTTACCCT TACCTTCTCT
    GCCCAGACCA AGACTTCCAA AGCCAAGAAG GATCAAGAAG GTGGAGAAGA AAAGAAATCT
    GTGCAAAAGA AAAAAGTAAA AGAGTTAAAG GTGTTGGATT CAAAGACAGC CCAGAATCTC
    TCAATCTTTT TGGGTTCCTT CCGCATGCCC TATCAAGAGA TTAAGAATGT CATCCTGGAG
    GTGAATGAGG CTGTTCTGAC TGAGTCTATG ATCCAGAACC TCATTAAGCA AATGCCAGAG
    CCAGAGCAGT TAAAAATGCT TTCTGAACTG AAGGATGAAT ATGATGACCT GGCTGAGTCA
    GAGCAGTTTG GCGTGGTGAT GGGCACTGTG CCCCGACTGC GGCCTCGCCT CAATGCCATT
    CTCTTCAAGC TACAATTCAG CGAGCAAGTG GAGAATATCA AGCCAGAGAT TGTGTCTGTC
    ACTGCTGCAT GTGAGGAGTT ACGTAAGAGT GAGAGCTTTT CCAATCTCCT AGAGATTACC
    TTGCTTGTTG GAAATTACAT GAATGCTGGC TCCAGAAATG CTGGTGCTTT TGGCTTCAAT
    ATCAGCTTCC TCTGTAAGCT TCGAGACACC AAGTCCACAG ATCAGAAGAT GACGTTGTTA
    CACTTCTTGG CTGAGTTGTG TGAGAATGAC TATCCCGATG TCCTCAAGTT TCCAGACGAG
    CTTGCCCATG TGGAGAAAGC CAGCCGAGTT TCTGCTGAAA ACTTGCAAAA GAACCTAGAT
    CAGATGAAGA AACAAATTTC TGATGTGGAA CGTGATGTTC AGAATTTCCC AGCTGCCACA
    GATGAAAAAG ACAAGTTTGT TGAAAAAATG ACCAGCTTTG TGAAGGATGC ACAGGAACAG
    TATAACAAGC TGCGGATGAT GCATTCTAAC ATGGAGACCC TCTATAAGGA GCTGGGCGAG
    TACTTCCTCT TTGACCCCAA GAAGTTGTCT GTTGAAGAAT TTTTCATGGA TCTTCACAAT
    TTTCGGAATA TGTTTTTGCA AGCAGTCAAG GAGAACCAGA AGCGGCGGGA GACAGAAGAA
    AAGATGAGGC GAGCAAAACT AGCCAAGGAG AAGGCAGAGA AGGAGCGGCT AGAGAAGCAG
    CAGAAGAGAG AGCAACTCAT AGACATGAAT GCAGAGGGCG ATGAGACAGG TGTGATGGAC
    AGTCTTCTAG AAGCCCTGCA GTCAGGGGCA GCATTCCGAC GGAAGAGAGG GCCCCGTCAA
    GCCAACAGGA AGGCCGGGTG TGCAGTCACA TCTCTGCTAG CTTCGGAGCT GACCAAGGAT
    GATGCCATGG CTGCTGTTCC TGCCAAGGTG TCCAAGAACA GTGAGACATT CCCCACAATC
    CTTGAGGAAG CCAAGGAGTT GGTTGGCCGT GCAAGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu105637
    Clone ID Related Accession (Same CDS sequence) XM_024454384.1
    Accession Version XM_024454384.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3942bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product protein diaphanous homolog 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGAGCCGC CCGGCGGGAG CCTGGGGCCC GGCCGCGGGA CCCGGGACAA GAAGAAGGGC 
    CGGAGCCCAG ATGAGCTGCC CTCGGCGGGC GGCGACGGCG GCAAATCTAA GAAATTTACT
    CTGAAGCGGC TCATGGCAGA TGAGCTGGAG AGATTTACCA GCATGAGAAT TAAGAAGGAG
    AAGGAAAAGC CCAATTCTGC TCATAGAAAT TCTTCTGCAT CATATGGGGA TGATCCCACA
    GCACAGTCAT TGCAAGATGT TTCAGATGAA CAAGTGCTGG TTCTCTTTGA ACAGATGCTG
    CTGGATATGA ACCTGAATGA GGAGAAACAG CAACCTTTGA GGGAGAAGGA CATCATCATC
    AAGAGGGAGA TGGTGTCCCA ATACTTGTAC ACCTCCAAGG CTGGCATGAG CCAGAAGGAG
    AGCTCTAAGT CTGCCATGAT GTATATTCAG GAGTTGAGGT CAGGCTTGCG GGATATGCCT
    CTGCTCAGCT GCCTGGAGTC CCTTCGTGTG TCTCTCAACA ACAACCCTGT CAGTTGGGTG
    CAAACATTTG GTGCTGAAGG CTTGGCCTCC TTATTGGACA TTCTTAAACG ACTTCATGAT
    GAGAAAGAAG AGACTGCTGG GAGTTACGAT AGCCGGAACA AGCATGAGAT CATTCGCTGC
    TTGAAAGCTT TTATGAACAA CAAGTTTGGA ATCAAGACCA TGTTGGAGAC AGAAGAAGGA
    ATCCTACTGC TGGTCAGAGC CATGGATCCT GCTGTTCCCA ACATGATGAT TGATGCAGCT
    AAGCTGCTTT CTGCTCTTTG TATTCTACCG CAGCCAGAGG ACATGAATGA AAGGGTTTTG
    GAGGCAATGA CAGAAAGAGC TGAGATGGAT GAAGTGGAAC GTTTCCAGCC GCTGCTGGAT
    GGATTAAAAA GTGGAACCAC TATTGCACTG AAGGTTGGAT GCCTACAGCT GATCAATGCT
    CTCATCACAC CAGCGGAGGA ACTTGACTTC CGAGTTCACA TCAGAAGTGA ACTGATGCGT
    TTGGGGCTAC ATCAGGTGTT GCAGGACCTT CGAGAGATTG AAAATGAAGA TATGAGAGTG
    CAACTAAATG TGTTTGATGA ACAAGGGGAA GAGGATTCCT ATGACCTGAA GGGACGGCTG
    GATGACATTC GCATGGAGAT GGATGACTTT AATGAAGTCT TTCAGATTCT CTTAAACACA
    GTGAAGGATT CAAAGGCAGA GCCACACTTC CTTTCCATCC TGCAGCACTT ACTCTTGGTC
    CGAAATGACT ATGAGGCCAG ACCTCAGTAC TATAAGTTGA TTGAAGAATG TATTTCCCAG
    ATAGTTCTGC ACAAGAACGG GGCTGATCCT GACTTCAAGT GCCGGCACCT CCAGATTGAG
    ATTGAGGGAT TAATTGATCA AATGATTGAT AAGACAAAGG TGGAGAAATC TGAAGCCAAA
    GCTGCAGAGC TGGAAAAGAA GTTGGACTCA GAGTTAACAG CCCGACATGA GCTACAGGTG
    GAAATGAAAA AGATGGAAAG TGACTTTGAG CAGAAGCTTC AAGATCTTCA GGGAGAAAAA
    GATGCACTGC ATTCTGAAAA GCAGCAAATT GCCACAGAGA AACAGGACCT GGAAGCAGAG
    GTGTCCCAGC TCACAGGAGA GGTTGCCAAG CTGACAAAGG AACTGGAAGA TGCCAAGAAA
    GAAATGGCTT CCCTCTCTGC GGCAGCTATT ACTGTACCTC CTTCTGTTCC TAGTCGTGCT
    CCTGTTCCCC CTGCCCCTCC TTTACCTGGT GACTCTGGCA CTATTATTCC ACCACCACCT
    GCTCCTGGGG ATAGTACCAC TCCTCCTCCT CCTCCTCCTC CTCCTCCTCC TCCACCTCCT
    TTGCCTGGGG GTGTTTGCAT CTCCTCACCC CCTTCTTTAC CTGGAGGTAC TGCTATCTCT
    CCACCCCCTC CTTTGTCTGG GGATGCTACC ATCCCTCCAC CCCCTCCTTT GCCTGAGGGT
    GTTGGCATCC CTTCACCCTC TTCTTTGCCT GGAGGTACTG CCATCCCCCC ACCTCCTCCT
    TTGCCTGGGA GTGCTAGAAT CCCCCCACCA CCACCTCCTT TGCCTGGGAG TGCTGGAATT
    CCCCCCCCAC CTCCTCCCTT GCCTGGAGAA GCAGGAATGC CACCTCCTCC TCCCCCTCTT
    CCTGGTGGTC CTGGAATCCC TCCACCTCCT CCATTTCCCG GAGGCCCTGG CATTCCTCCA
    CCTCCACCCG GAATGGGTAT GCCTCCACCT CCCCCATTTG GATTTGGAGT TCCTGCAGCC
    CCAGTTCTGC CATTTGGATT AACCCCCAAA AAGCTTTATA AGCCAGAGGT GCAGCTCCGG
    AGGCCAAACT GGTCCAAGCT TGTGGCTGAG GACCTCTCCC AGGACTGCTT CTGGACAAAG
    GTGAAGGAGG ACCGCTTTGA GAACAATGAA CTTTTCGCCA AACTTACCCT TACCTTCTCT
    GCCCAGACCA AGACTTCCAA AGCCAAGAAG GATCAAGAAG GTGGAGAAGA AAAGAAATCT
    GTGCAAAAGA AAAAAGTAAA AGAGTTAAAG GTGTTGGATT CAAAGACAGC CCAGAATCTC
    TCAATCTTTT TGGGTTCCTT CCGCATGCCC TATCAAGAGA TTAAGAATGT CATCCTGGAG
    GTGAATGAGG CTGTTCTGAC TGAGTCTATG ATCCAGAACC TCATTAAGCA AATGCCAGAG
    CCAGAGCAGT TAAAAATGCT TTCTGAACTG AAGGATGAAT ATGATGACCT GGCTGAGTCA
    GAGCAGTTTG GCGTGGTGAT GGGCACTGTG CCCCGACTGC GGCCTCGCCT CAATGCCATT
    CTCTTCAAGC TACAATTCAG CGAGCAAGTG GAGAATATCA AGCCAGAGAT TGTGTCTGTC
    ACTGCTGCAT GTGAGGAGTT ACGTAAGAGT GAGAGCTTTT CCAATCTCCT AGAGATTACC
    TTGCTTGTTG GAAATTACAT GAATGCTGGC TCCAGAAATG CTGGTGCTTT TGGCTTCAAT
    ATCAGCTTCC TCTGTAAGCT TCGAGACACC AAGTCCACAG ATCAGAAGAT GACGTTGTTA
    CACTTCTTGG CTGAGTTGTG TGAGAATGAC TATCCCGATG TCCTCAAGTT TCCAGACGAG
    CTTGCCCATG TGGAGAAAGC CAGCCGAGTT TCTGCTGAAA ACTTGCAAAA GAACCTAGAT
    CAGATGAAGA AACAAATTTC TGATGTGGAA CGTGATGTTC AGAATTTCCC AGCTGCCACA
    GATGAAAAAG ACAAGTTTGT TGAAAAAATG ACCAGCTTTG TGAAGGATGC ACAGGAACAG
    TATAACAAGC TGCGGATGAT GCATTCTAAC ATGGAGACCC TCTATAAGGA GCTGGGCGAG
    TACTTCCTCT TTGACCCCAA GAAGTTGTCT GTTGAAGAAT TTTTCATGGA TCTTCACAAT
    TTTCGGAATA TGTTTTTGGT GAGCAAGGGA TACTTGCTGA CTGAATCTTT TGTGGGCATT
    TCTCACTTTC CCCTGGGTTC CTGTTGGCTG GTAGTAGGTA TACAATGCCC ATGTGTTCCT
    TCCTCCTCTT CCCTGCCCCA GCAAGCAGTC AAGGAGAACC AGAAGCGGCG GGAGACAGAA
    GAAAAGATGA GGCGAGCAAA ACTAGCCAAG GAGAAGGCAG AGAAGGAGCG GCTAGAGAAG
    CAGCAGAAGA GAGAGCAACT CATAGACATG AATGCAGAGG GCGATGAGAC AGGTGTGATG
    GACAGTCTTC TAGAAGCCCT GCAGTCAGGG GCAGCATTCC GACGGAAGAG AGGGCCCCGT
    CAAGCCAACA GGAAGGCCGG GTGTGCAGTC ACATCTCTGC TAGCTTCGGA GCTGACCAAG
    GATGATGCCA TGGCTGCTGT TCCTGCCAAG GTGTCCAAGA ACAGTGAGAC ATTCCCCACA
    ATCCTTGAGG AAGCCAAGGA GTTGGTTGGC CGTGCAAGCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024310152.1
    CDS142..4083
    Translation

    Target ORF information:

    RefSeq Version XM_024454384.1
    Organism Homo sapiens(human)
    Definition Homo sapiens diaphanous related formin 1 (DIAPH1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024454384.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGAGCCGC CCGGCGGGAG CCTGGGGCCC GGCCGCGGGA CCCGGGACAA GAAGAAGGGC 
    CGGAGCCCAG ATGAGCTGCC CTCGGCGGGC GGCGACGGCG GCAAATCTAA GAAATTTACT
    CTGAAGCGGC TCATGGCAGA TGAGCTGGAG AGATTTACCA GCATGAGAAT TAAGAAGGAG
    AAGGAAAAGC CCAATTCTGC TCATAGAAAT TCTTCTGCAT CATATGGGGA TGATCCCACA
    GCACAGTCAT TGCAAGATGT TTCAGATGAA CAAGTGCTGG TTCTCTTTGA ACAGATGCTG
    CTGGATATGA ACCTGAATGA GGAGAAACAG CAACCTTTGA GGGAGAAGGA CATCATCATC
    AAGAGGGAGA TGGTGTCCCA ATACTTGTAC ACCTCCAAGG CTGGCATGAG CCAGAAGGAG
    AGCTCTAAGT CTGCCATGAT GTATATTCAG GAGTTGAGGT CAGGCTTGCG GGATATGCCT
    CTGCTCAGCT GCCTGGAGTC CCTTCGTGTG TCTCTCAACA ACAACCCTGT CAGTTGGGTG
    CAAACATTTG GTGCTGAAGG CTTGGCCTCC TTATTGGACA TTCTTAAACG ACTTCATGAT
    GAGAAAGAAG AGACTGCTGG GAGTTACGAT AGCCGGAACA AGCATGAGAT CATTCGCTGC
    TTGAAAGCTT TTATGAACAA CAAGTTTGGA ATCAAGACCA TGTTGGAGAC AGAAGAAGGA
    ATCCTACTGC TGGTCAGAGC CATGGATCCT GCTGTTCCCA ACATGATGAT TGATGCAGCT
    AAGCTGCTTT CTGCTCTTTG TATTCTACCG CAGCCAGAGG ACATGAATGA AAGGGTTTTG
    GAGGCAATGA CAGAAAGAGC TGAGATGGAT GAAGTGGAAC GTTTCCAGCC GCTGCTGGAT
    GGATTAAAAA GTGGAACCAC TATTGCACTG AAGGTTGGAT GCCTACAGCT GATCAATGCT
    CTCATCACAC CAGCGGAGGA ACTTGACTTC CGAGTTCACA TCAGAAGTGA ACTGATGCGT
    TTGGGGCTAC ATCAGGTGTT GCAGGACCTT CGAGAGATTG AAAATGAAGA TATGAGAGTG
    CAACTAAATG TGTTTGATGA ACAAGGGGAA GAGGATTCCT ATGACCTGAA GGGACGGCTG
    GATGACATTC GCATGGAGAT GGATGACTTT AATGAAGTCT TTCAGATTCT CTTAAACACA
    GTGAAGGATT CAAAGGCAGA GCCACACTTC CTTTCCATCC TGCAGCACTT ACTCTTGGTC
    CGAAATGACT ATGAGGCCAG ACCTCAGTAC TATAAGTTGA TTGAAGAATG TATTTCCCAG
    ATAGTTCTGC ACAAGAACGG GGCTGATCCT GACTTCAAGT GCCGGCACCT CCAGATTGAG
    ATTGAGGGAT TAATTGATCA AATGATTGAT AAGACAAAGG TGGAGAAATC TGAAGCCAAA
    GCTGCAGAGC TGGAAAAGAA GTTGGACTCA GAGTTAACAG CCCGACATGA GCTACAGGTG
    GAAATGAAAA AGATGGAAAG TGACTTTGAG CAGAAGCTTC AAGATCTTCA GGGAGAAAAA
    GATGCACTGC ATTCTGAAAA GCAGCAAATT GCCACAGAGA AACAGGACCT GGAAGCAGAG
    GTGTCCCAGC TCACAGGAGA GGTTGCCAAG CTGACAAAGG AACTGGAAGA TGCCAAGAAA
    GAAATGGCTT CCCTCTCTGC GGCAGCTATT ACTGTACCTC CTTCTGTTCC TAGTCGTGCT
    CCTGTTCCCC CTGCCCCTCC TTTACCTGGT GACTCTGGCA CTATTATTCC ACCACCACCT
    GCTCCTGGGG ATAGTACCAC TCCTCCTCCT CCTCCTCCTC CTCCTCCTCC TCCACCTCCT
    TTGCCTGGGG GTGTTTGCAT CTCCTCACCC CCTTCTTTAC CTGGAGGTAC TGCTATCTCT
    CCACCCCCTC CTTTGTCTGG GGATGCTACC ATCCCTCCAC CCCCTCCTTT GCCTGAGGGT
    GTTGGCATCC CTTCACCCTC TTCTTTGCCT GGAGGTACTG CCATCCCCCC ACCTCCTCCT
    TTGCCTGGGA GTGCTAGAAT CCCCCCACCA CCACCTCCTT TGCCTGGGAG TGCTGGAATT
    CCCCCCCCAC CTCCTCCCTT GCCTGGAGAA GCAGGAATGC CACCTCCTCC TCCCCCTCTT
    CCTGGTGGTC CTGGAATCCC TCCACCTCCT CCATTTCCCG GAGGCCCTGG CATTCCTCCA
    CCTCCACCCG GAATGGGTAT GCCTCCACCT CCCCCATTTG GATTTGGAGT TCCTGCAGCC
    CCAGTTCTGC CATTTGGATT AACCCCCAAA AAGCTTTATA AGCCAGAGGT GCAGCTCCGG
    AGGCCAAACT GGTCCAAGCT TGTGGCTGAG GACCTCTCCC AGGACTGCTT CTGGACAAAG
    GTGAAGGAGG ACCGCTTTGA GAACAATGAA CTTTTCGCCA AACTTACCCT TACCTTCTCT
    GCCCAGACCA AGACTTCCAA AGCCAAGAAG GATCAAGAAG GTGGAGAAGA AAAGAAATCT
    GTGCAAAAGA AAAAAGTAAA AGAGTTAAAG GTGTTGGATT CAAAGACAGC CCAGAATCTC
    TCAATCTTTT TGGGTTCCTT CCGCATGCCC TATCAAGAGA TTAAGAATGT CATCCTGGAG
    GTGAATGAGG CTGTTCTGAC TGAGTCTATG ATCCAGAACC TCATTAAGCA AATGCCAGAG
    CCAGAGCAGT TAAAAATGCT TTCTGAACTG AAGGATGAAT ATGATGACCT GGCTGAGTCA
    GAGCAGTTTG GCGTGGTGAT GGGCACTGTG CCCCGACTGC GGCCTCGCCT CAATGCCATT
    CTCTTCAAGC TACAATTCAG CGAGCAAGTG GAGAATATCA AGCCAGAGAT TGTGTCTGTC
    ACTGCTGCAT GTGAGGAGTT ACGTAAGAGT GAGAGCTTTT CCAATCTCCT AGAGATTACC
    TTGCTTGTTG GAAATTACAT GAATGCTGGC TCCAGAAATG CTGGTGCTTT TGGCTTCAAT
    ATCAGCTTCC TCTGTAAGCT TCGAGACACC AAGTCCACAG ATCAGAAGAT GACGTTGTTA
    CACTTCTTGG CTGAGTTGTG TGAGAATGAC TATCCCGATG TCCTCAAGTT TCCAGACGAG
    CTTGCCCATG TGGAGAAAGC CAGCCGAGTT TCTGCTGAAA ACTTGCAAAA GAACCTAGAT
    CAGATGAAGA AACAAATTTC TGATGTGGAA CGTGATGTTC AGAATTTCCC AGCTGCCACA
    GATGAAAAAG ACAAGTTTGT TGAAAAAATG ACCAGCTTTG TGAAGGATGC ACAGGAACAG
    TATAACAAGC TGCGGATGAT GCATTCTAAC ATGGAGACCC TCTATAAGGA GCTGGGCGAG
    TACTTCCTCT TTGACCCCAA GAAGTTGTCT GTTGAAGAAT TTTTCATGGA TCTTCACAAT
    TTTCGGAATA TGTTTTTGGT GAGCAAGGGA TACTTGCTGA CTGAATCTTT TGTGGGCATT
    TCTCACTTTC CCCTGGGTTC CTGTTGGCTG GTAGTAGGTA TACAATGCCC ATGTGTTCCT
    TCCTCCTCTT CCCTGCCCCA GCAAGCAGTC AAGGAGAACC AGAAGCGGCG GGAGACAGAA
    GAAAAGATGA GGCGAGCAAA ACTAGCCAAG GAGAAGGCAG AGAAGGAGCG GCTAGAGAAG
    CAGCAGAAGA GAGAGCAACT CATAGACATG AATGCAGAGG GCGATGAGAC AGGTGTGATG
    GACAGTCTTC TAGAAGCCCT GCAGTCAGGG GCAGCATTCC GACGGAAGAG AGGGCCCCGT
    CAAGCCAACA GGAAGGCCGG GTGTGCAGTC ACATCTCTGC TAGCTTCGGA GCTGACCAAG
    GATGATGCCA TGGCTGCTGT TCCTGCCAAG GTGTCCAAGA ACAGTGAGAC ATTCCCCACA
    ATCCTTGAGG AAGCCAAGGA GTTGGTTGGC CGTGCAAGCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu105638
    Clone ID Related Accession (Same CDS sequence) XM_024454385.1
    Accession Version XM_024454385.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3915bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product protein diaphanous homolog 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGAGCCGC CCGGCGGGAG CCTGGGGCCC GGCCGCGGGA CCCGGGACAA GAAGAAGGGC 
    CGGAGCCCAG ATGAGCTGCC CTCGGCGGGC GGCGACGGCG GCAAATCTAA GAAATTTCTG
    GAGAGATTTA CCAGCATGAG AATTAAGAAG GAGAAGGAAA AGCCCAATTC TGCTCATAGA
    AATTCTTCTG CATCATATGG GGATGATCCC ACAGCACAGT CATTGCAAGA TGTTTCAGAT
    GAACAAGTGC TGGTTCTCTT TGAACAGATG CTGCTGGATA TGAACCTGAA TGAGGAGAAA
    CAGCAACCTT TGAGGGAGAA GGACATCATC ATCAAGAGGG AGATGGTGTC CCAATACTTG
    TACACCTCCA AGGCTGGCAT GAGCCAGAAG GAGAGCTCTA AGTCTGCCAT GATGTATATT
    CAGGAGTTGA GGTCAGGCTT GCGGGATATG CCTCTGCTCA GCTGCCTGGA GTCCCTTCGT
    GTGTCTCTCA ACAACAACCC TGTCAGTTGG GTGCAAACAT TTGGTGCTGA AGGCTTGGCC
    TCCTTATTGG ACATTCTTAA ACGACTTCAT GATGAGAAAG AAGAGACTGC TGGGAGTTAC
    GATAGCCGGA ACAAGCATGA GATCATTCGC TGCTTGAAAG CTTTTATGAA CAACAAGTTT
    GGAATCAAGA CCATGTTGGA GACAGAAGAA GGAATCCTAC TGCTGGTCAG AGCCATGGAT
    CCTGCTGTTC CCAACATGAT GATTGATGCA GCTAAGCTGC TTTCTGCTCT TTGTATTCTA
    CCGCAGCCAG AGGACATGAA TGAAAGGGTT TTGGAGGCAA TGACAGAAAG AGCTGAGATG
    GATGAAGTGG AACGTTTCCA GCCGCTGCTG GATGGATTAA AAAGTGGAAC CACTATTGCA
    CTGAAGGTTG GATGCCTACA GCTGATCAAT GCTCTCATCA CACCAGCGGA GGAACTTGAC
    TTCCGAGTTC ACATCAGAAG TGAACTGATG CGTTTGGGGC TACATCAGGT GTTGCAGGAC
    CTTCGAGAGA TTGAAAATGA AGATATGAGA GTGCAACTAA ATGTGTTTGA TGAACAAGGG
    GAAGAGGATT CCTATGACCT GAAGGGACGG CTGGATGACA TTCGCATGGA GATGGATGAC
    TTTAATGAAG TCTTTCAGAT TCTCTTAAAC ACAGTGAAGG ATTCAAAGGC AGAGCCACAC
    TTCCTTTCCA TCCTGCAGCA CTTACTCTTG GTCCGAAATG ACTATGAGGC CAGACCTCAG
    TACTATAAGT TGATTGAAGA ATGTATTTCC CAGATAGTTC TGCACAAGAA CGGGGCTGAT
    CCTGACTTCA AGTGCCGGCA CCTCCAGATT GAGATTGAGG GATTAATTGA TCAAATGATT
    GATAAGACAA AGGTGGAGAA ATCTGAAGCC AAAGCTGCAG AGCTGGAAAA GAAGTTGGAC
    TCAGAGTTAA CAGCCCGACA TGAGCTACAG GTGGAAATGA AAAAGATGGA AAGTGACTTT
    GAGCAGAAGC TTCAAGATCT TCAGGGAGAA AAAGATGCAC TGCATTCTGA AAAGCAGCAA
    ATTGCCACAG AGAAACAGGA CCTGGAAGCA GAGGTGTCCC AGCTCACAGG AGAGGTTGCC
    AAGCTGACAA AGGAACTGGA AGATGCCAAG AAAGAAATGG CTTCCCTCTC TGCGGCAGCT
    ATTACTGTAC CTCCTTCTGT TCCTAGTCGT GCTCCTGTTC CCCCTGCCCC TCCTTTACCT
    GGTGACTCTG GCACTATTAT TCCACCACCA CCTGCTCCTG GGGATAGTAC CACTCCTCCT
    CCTCCTCCTC CTCCTCCTCC TCCTCCACCT CCTTTGCCTG GGGGTGTTTG CATCTCCTCA
    CCCCCTTCTT TACCTGGAGG TACTGCTATC TCTCCACCCC CTCCTTTGTC TGGGGATGCT
    ACCATCCCTC CACCCCCTCC TTTGCCTGAG GGTGTTGGCA TCCCTTCACC CTCTTCTTTG
    CCTGGAGGTA CTGCCATCCC CCCACCTCCT CCTTTGCCTG GGAGTGCTAG AATCCCCCCA
    CCACCACCTC CTTTGCCTGG GAGTGCTGGA ATTCCCCCCC CACCTCCTCC CTTGCCTGGA
    GAAGCAGGAA TGCCACCTCC TCCTCCCCCT CTTCCTGGTG GTCCTGGAAT CCCTCCACCT
    CCTCCATTTC CCGGAGGCCC TGGCATTCCT CCACCTCCAC CCGGAATGGG TATGCCTCCA
    CCTCCCCCAT TTGGATTTGG AGTTCCTGCA GCCCCAGTTC TGCCATTTGG ATTAACCCCC
    AAAAAGCTTT ATAAGCCAGA GGTGCAGCTC CGGAGGCCAA ACTGGTCCAA GCTTGTGGCT
    GAGGACCTCT CCCAGGACTG CTTCTGGACA AAGGTGAAGG AGGACCGCTT TGAGAACAAT
    GAACTTTTCG CCAAACTTAC CCTTACCTTC TCTGCCCAGA CCAAGACTTC CAAAGCCAAG
    AAGGATCAAG AAGGTGGAGA AGAAAAGAAA TCTGTGCAAA AGAAAAAAGT AAAAGAGTTA
    AAGGTGTTGG ATTCAAAGAC AGCCCAGAAT CTCTCAATCT TTTTGGGTTC CTTCCGCATG
    CCCTATCAAG AGATTAAGAA TGTCATCCTG GAGGTGAATG AGGCTGTTCT GACTGAGTCT
    ATGATCCAGA ACCTCATTAA GCAAATGCCA GAGCCAGAGC AGTTAAAAAT GCTTTCTGAA
    CTGAAGGATG AATATGATGA CCTGGCTGAG TCAGAGCAGT TTGGCGTGGT GATGGGCACT
    GTGCCCCGAC TGCGGCCTCG CCTCAATGCC ATTCTCTTCA AGCTACAATT CAGCGAGCAA
    GTGGAGAATA TCAAGCCAGA GATTGTGTCT GTCACTGCTG CATGTGAGGA GTTACGTAAG
    AGTGAGAGCT TTTCCAATCT CCTAGAGATT ACCTTGCTTG TTGGAAATTA CATGAATGCT
    GGCTCCAGAA ATGCTGGTGC TTTTGGCTTC AATATCAGCT TCCTCTGTAA GCTTCGAGAC
    ACCAAGTCCA CAGATCAGAA GATGACGTTG TTACACTTCT TGGCTGAGTT GTGTGAGAAT
    GACTATCCCG ATGTCCTCAA GTTTCCAGAC GAGCTTGCCC ATGTGGAGAA AGCCAGCCGA
    GTTTCTGCTG AAAACTTGCA AAAGAACCTA GATCAGATGA AGAAACAAAT TTCTGATGTG
    GAACGTGATG TTCAGAATTT CCCAGCTGCC ACAGATGAAA AAGACAAGTT TGTTGAAAAA
    ATGACCAGCT TTGTGAAGGA TGCACAGGAA CAGTATAACA AGCTGCGGAT GATGCATTCT
    AACATGGAGA CCCTCTATAA GGAGCTGGGC GAGTACTTCC TCTTTGACCC CAAGAAGTTG
    TCTGTTGAAG AATTTTTCAT GGATCTTCAC AATTTTCGGA ATATGTTTTT GGTGAGCAAG
    GGATACTTGC TGACTGAATC TTTTGTGGGC ATTTCTCACT TTCCCCTGGG TTCCTGTTGG
    CTGGTAGTAG GTATACAATG CCCATGTGTT CCTTCCTCCT CTTCCCTGCC CCAGCAAGCA
    GTCAAGGAGA ACCAGAAGCG GCGGGAGACA GAAGAAAAGA TGAGGCGAGC AAAACTAGCC
    AAGGAGAAGG CAGAGAAGGA GCGGCTAGAG AAGCAGCAGA AGAGAGAGCA ACTCATAGAC
    ATGAATGCAG AGGGCGATGA GACAGGTGTG ATGGACAGTC TTCTAGAAGC CCTGCAGTCA
    GGGGCAGCAT TCCGACGGAA GAGAGGGCCC CGTCAAGCCA ACAGGAAGGC CGGGTGTGCA
    GTCACATCTC TGCTAGCTTC GGAGCTGACC AAGGATGATG CCATGGCTGC TGTTCCTGCC
    AAGGTGTCCA AGAACAGTGA GACATTCCCC ACAATCCTTG AGGAAGCCAA GGAGTTGGTT
    GGCCGTGCAA GCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024310153.1
    CDS93..4007
    Translation

    Target ORF information:

    RefSeq Version XM_024454385.1
    Organism Homo sapiens(human)
    Definition Homo sapiens diaphanous related formin 1 (DIAPH1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024454385.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGAGCCGC CCGGCGGGAG CCTGGGGCCC GGCCGCGGGA CCCGGGACAA GAAGAAGGGC 
    CGGAGCCCAG ATGAGCTGCC CTCGGCGGGC GGCGACGGCG GCAAATCTAA GAAATTTCTG
    GAGAGATTTA CCAGCATGAG AATTAAGAAG GAGAAGGAAA AGCCCAATTC TGCTCATAGA
    AATTCTTCTG CATCATATGG GGATGATCCC ACAGCACAGT CATTGCAAGA TGTTTCAGAT
    GAACAAGTGC TGGTTCTCTT TGAACAGATG CTGCTGGATA TGAACCTGAA TGAGGAGAAA
    CAGCAACCTT TGAGGGAGAA GGACATCATC ATCAAGAGGG AGATGGTGTC CCAATACTTG
    TACACCTCCA AGGCTGGCAT GAGCCAGAAG GAGAGCTCTA AGTCTGCCAT GATGTATATT
    CAGGAGTTGA GGTCAGGCTT GCGGGATATG CCTCTGCTCA GCTGCCTGGA GTCCCTTCGT
    GTGTCTCTCA ACAACAACCC TGTCAGTTGG GTGCAAACAT TTGGTGCTGA AGGCTTGGCC
    TCCTTATTGG ACATTCTTAA ACGACTTCAT GATGAGAAAG AAGAGACTGC TGGGAGTTAC
    GATAGCCGGA ACAAGCATGA GATCATTCGC TGCTTGAAAG CTTTTATGAA CAACAAGTTT
    GGAATCAAGA CCATGTTGGA GACAGAAGAA GGAATCCTAC TGCTGGTCAG AGCCATGGAT
    CCTGCTGTTC CCAACATGAT GATTGATGCA GCTAAGCTGC TTTCTGCTCT TTGTATTCTA
    CCGCAGCCAG AGGACATGAA TGAAAGGGTT TTGGAGGCAA TGACAGAAAG AGCTGAGATG
    GATGAAGTGG AACGTTTCCA GCCGCTGCTG GATGGATTAA AAAGTGGAAC CACTATTGCA
    CTGAAGGTTG GATGCCTACA GCTGATCAAT GCTCTCATCA CACCAGCGGA GGAACTTGAC
    TTCCGAGTTC ACATCAGAAG TGAACTGATG CGTTTGGGGC TACATCAGGT GTTGCAGGAC
    CTTCGAGAGA TTGAAAATGA AGATATGAGA GTGCAACTAA ATGTGTTTGA TGAACAAGGG
    GAAGAGGATT CCTATGACCT GAAGGGACGG CTGGATGACA TTCGCATGGA GATGGATGAC
    TTTAATGAAG TCTTTCAGAT TCTCTTAAAC ACAGTGAAGG ATTCAAAGGC AGAGCCACAC
    TTCCTTTCCA TCCTGCAGCA CTTACTCTTG GTCCGAAATG ACTATGAGGC CAGACCTCAG
    TACTATAAGT TGATTGAAGA ATGTATTTCC CAGATAGTTC TGCACAAGAA CGGGGCTGAT
    CCTGACTTCA AGTGCCGGCA CCTCCAGATT GAGATTGAGG GATTAATTGA TCAAATGATT
    GATAAGACAA AGGTGGAGAA ATCTGAAGCC AAAGCTGCAG AGCTGGAAAA GAAGTTGGAC
    TCAGAGTTAA CAGCCCGACA TGAGCTACAG GTGGAAATGA AAAAGATGGA AAGTGACTTT
    GAGCAGAAGC TTCAAGATCT TCAGGGAGAA AAAGATGCAC TGCATTCTGA AAAGCAGCAA
    ATTGCCACAG AGAAACAGGA CCTGGAAGCA GAGGTGTCCC AGCTCACAGG AGAGGTTGCC
    AAGCTGACAA AGGAACTGGA AGATGCCAAG AAAGAAATGG CTTCCCTCTC TGCGGCAGCT
    ATTACTGTAC CTCCTTCTGT TCCTAGTCGT GCTCCTGTTC CCCCTGCCCC TCCTTTACCT
    GGTGACTCTG GCACTATTAT TCCACCACCA CCTGCTCCTG GGGATAGTAC CACTCCTCCT
    CCTCCTCCTC CTCCTCCTCC TCCTCCACCT CCTTTGCCTG GGGGTGTTTG CATCTCCTCA
    CCCCCTTCTT TACCTGGAGG TACTGCTATC TCTCCACCCC CTCCTTTGTC TGGGGATGCT
    ACCATCCCTC CACCCCCTCC TTTGCCTGAG GGTGTTGGCA TCCCTTCACC CTCTTCTTTG
    CCTGGAGGTA CTGCCATCCC CCCACCTCCT CCTTTGCCTG GGAGTGCTAG AATCCCCCCA
    CCACCACCTC CTTTGCCTGG GAGTGCTGGA ATTCCCCCCC CACCTCCTCC CTTGCCTGGA
    GAAGCAGGAA TGCCACCTCC TCCTCCCCCT CTTCCTGGTG GTCCTGGAAT CCCTCCACCT
    CCTCCATTTC CCGGAGGCCC TGGCATTCCT CCACCTCCAC CCGGAATGGG TATGCCTCCA
    CCTCCCCCAT TTGGATTTGG AGTTCCTGCA GCCCCAGTTC TGCCATTTGG ATTAACCCCC
    AAAAAGCTTT ATAAGCCAGA GGTGCAGCTC CGGAGGCCAA ACTGGTCCAA GCTTGTGGCT
    GAGGACCTCT CCCAGGACTG CTTCTGGACA AAGGTGAAGG AGGACCGCTT TGAGAACAAT
    GAACTTTTCG CCAAACTTAC CCTTACCTTC TCTGCCCAGA CCAAGACTTC CAAAGCCAAG
    AAGGATCAAG AAGGTGGAGA AGAAAAGAAA TCTGTGCAAA AGAAAAAAGT AAAAGAGTTA
    AAGGTGTTGG ATTCAAAGAC AGCCCAGAAT CTCTCAATCT TTTTGGGTTC CTTCCGCATG
    CCCTATCAAG AGATTAAGAA TGTCATCCTG GAGGTGAATG AGGCTGTTCT GACTGAGTCT
    ATGATCCAGA ACCTCATTAA GCAAATGCCA GAGCCAGAGC AGTTAAAAAT GCTTTCTGAA
    CTGAAGGATG AATATGATGA CCTGGCTGAG TCAGAGCAGT TTGGCGTGGT GATGGGCACT
    GTGCCCCGAC TGCGGCCTCG CCTCAATGCC ATTCTCTTCA AGCTACAATT CAGCGAGCAA
    GTGGAGAATA TCAAGCCAGA GATTGTGTCT GTCACTGCTG CATGTGAGGA GTTACGTAAG
    AGTGAGAGCT TTTCCAATCT CCTAGAGATT ACCTTGCTTG TTGGAAATTA CATGAATGCT
    GGCTCCAGAA ATGCTGGTGC TTTTGGCTTC AATATCAGCT TCCTCTGTAA GCTTCGAGAC
    ACCAAGTCCA CAGATCAGAA GATGACGTTG TTACACTTCT TGGCTGAGTT GTGTGAGAAT
    GACTATCCCG ATGTCCTCAA GTTTCCAGAC GAGCTTGCCC ATGTGGAGAA AGCCAGCCGA
    GTTTCTGCTG AAAACTTGCA AAAGAACCTA GATCAGATGA AGAAACAAAT TTCTGATGTG
    GAACGTGATG TTCAGAATTT CCCAGCTGCC ACAGATGAAA AAGACAAGTT TGTTGAAAAA
    ATGACCAGCT TTGTGAAGGA TGCACAGGAA CAGTATAACA AGCTGCGGAT GATGCATTCT
    AACATGGAGA CCCTCTATAA GGAGCTGGGC GAGTACTTCC TCTTTGACCC CAAGAAGTTG
    TCTGTTGAAG AATTTTTCAT GGATCTTCAC AATTTTCGGA ATATGTTTTT GGTGAGCAAG
    GGATACTTGC TGACTGAATC TTTTGTGGGC ATTTCTCACT TTCCCCTGGG TTCCTGTTGG
    CTGGTAGTAG GTATACAATG CCCATGTGTT CCTTCCTCCT CTTCCCTGCC CCAGCAAGCA
    GTCAAGGAGA ACCAGAAGCG GCGGGAGACA GAAGAAAAGA TGAGGCGAGC AAAACTAGCC
    AAGGAGAAGG CAGAGAAGGA GCGGCTAGAG AAGCAGCAGA AGAGAGAGCA ACTCATAGAC
    ATGAATGCAG AGGGCGATGA GACAGGTGTG ATGGACAGTC TTCTAGAAGC CCTGCAGTCA
    GGGGCAGCAT TCCGACGGAA GAGAGGGCCC CGTCAAGCCA ACAGGAAGGC CGGGTGTGCA
    GTCACATCTC TGCTAGCTTC GGAGCTGACC AAGGATGATG CCATGGCTGC TGTTCCTGCC
    AAGGTGTCCA AGAACAGTGA GACATTCCCC ACAATCCTTG AGGAAGCCAA GGAGTTGGTT
    GGCCGTGCAA GCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu105639
    Clone ID Related Accession (Same CDS sequence) XM_024454386.1
    Accession Version XM_024454386.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3906bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product protein diaphanous homolog 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGAGAGTAC AGAGTCAGTA TGGTAAGTTA GATAGGAGGA AGACTGATTT CTTCTTTTCA 
    TGTCAGAAGA GTCTTGAACT GCTTGTGAAC CAGAGGAACC ATGCCTGTCT GGAGAGATTT
    ACCAGCATGA GAATTAAGAA GGAGAAGGAA AAGCCCAATT CTGCTCATAG AAATTCTTCT
    GCATCATATG GGGATGATCC CACAGCACAG TCATTGCAAG ATGTTTCAGA TGAACAAGTG
    CTGGTTCTCT TTGAACAGAT GCTGCTGGAT ATGAACCTGA ATGAGGAGAA ACAGCAACCT
    TTGAGGGAGA AGGACATCAT CATCAAGAGG GAGATGGTGT CCCAATACTT GTACACCTCC
    AAGGCTGGCA TGAGCCAGAA GGAGAGCTCT AAGTCTGCCA TGATGTATAT TCAGGAGTTG
    AGGTCAGGCT TGCGGGATAT GCCTCTGCTC AGCTGCCTGG AGTCCCTTCG TGTGTCTCTC
    AACAACAACC CTGTCAGTTG GGTGCAAACA TTTGGTGCTG AAGGCTTGGC CTCCTTATTG
    GACATTCTTA AACGACTTCA TGATGAGAAA GAAGAGACTG CTGGGAGTTA CGATAGCCGG
    AACAAGCATG AGATCATTCG CTGCTTGAAA GCTTTTATGA ACAACAAGTT TGGAATCAAG
    ACCATGTTGG AGACAGAAGA AGGAATCCTA CTGCTGGTCA GAGCCATGGA TCCTGCTGTT
    CCCAACATGA TGATTGATGC AGCTAAGCTG CTTTCTGCTC TTTGTATTCT ACCGCAGCCA
    GAGGACATGA ATGAAAGGGT TTTGGAGGCA ATGACAGAAA GAGCTGAGAT GGATGAAGTG
    GAACGTTTCC AGCCGCTGCT GGATGGATTA AAAAGTGGAA CCACTATTGC ACTGAAGGTT
    GGATGCCTAC AGCTGATCAA TGCTCTCATC ACACCAGCGG AGGAACTTGA CTTCCGAGTT
    CACATCAGAA GTGAACTGAT GCGTTTGGGG CTACATCAGG TGTTGCAGGA CCTTCGAGAG
    ATTGAAAATG AAGATATGAG AGTGCAACTA AATGTGTTTG ATGAACAAGG GGAAGAGGAT
    TCCTATGACC TGAAGGGACG GCTGGATGAC ATTCGCATGG AGATGGATGA CTTTAATGAA
    GTCTTTCAGA TTCTCTTAAA CACAGTGAAG GATTCAAAGG CAGAGCCACA CTTCCTTTCC
    ATCCTGCAGC ACTTACTCTT GGTCCGAAAT GACTATGAGG CCAGACCTCA GTACTATAAG
    TTGATTGAAG AATGTATTTC CCAGATAGTT CTGCACAAGA ACGGGGCTGA TCCTGACTTC
    AAGTGCCGGC ACCTCCAGAT TGAGATTGAG GGATTAATTG ATCAAATGAT TGATAAGACA
    AAGGTGGAGA AATCTGAAGC CAAAGCTGCA GAGCTGGAAA AGAAGTTGGA CTCAGAGTTA
    ACAGCCCGAC ATGAGCTACA GGTGGAAATG AAAAAGATGG AAAGTGACTT TGAGCAGAAG
    CTTCAAGATC TTCAGGGAGA AAAAGATGCA CTGCATTCTG AAAAGCAGCA AATTGCCACA
    GAGAAACAGG ACCTGGAAGC AGAGGTGTCC CAGCTCACAG GAGAGGTTGC CAAGCTGACA
    AAGGAACTGG AAGATGCCAA GAAAGAAATG GCTTCCCTCT CTGCGGCAGC TATTACTGTA
    CCTCCTTCTG TTCCTAGTCG TGCTCCTGTT CCCCCTGCCC CTCCTTTACC TGGTGACTCT
    GGCACTATTA TTCCACCACC ACCTGCTCCT GGGGATAGTA CCACTCCTCC TCCTCCTCCT
    CCTCCTCCTC CTCCTCCACC TCCTTTGCCT GGGGGTGTTT GCATCTCCTC ACCCCCTTCT
    TTACCTGGAG GTACTGCTAT CTCTCCACCC CCTCCTTTGT CTGGGGATGC TACCATCCCT
    CCACCCCCTC CTTTGCCTGA GGGTGTTGGC ATCCCTTCAC CCTCTTCTTT GCCTGGAGGT
    ACTGCCATCC CCCCACCTCC TCCTTTGCCT GGGAGTGCTA GAATCCCCCC ACCACCACCT
    CCTTTGCCTG GGAGTGCTGG AATTCCCCCC CCACCTCCTC CCTTGCCTGG AGAAGCAGGA
    ATGCCACCTC CTCCTCCCCC TCTTCCTGGT GGTCCTGGAA TCCCTCCACC TCCTCCATTT
    CCCGGAGGCC CTGGCATTCC TCCACCTCCA CCCGGAATGG GTATGCCTCC ACCTCCCCCA
    TTTGGATTTG GAGTTCCTGC AGCCCCAGTT CTGCCATTTG GATTAACCCC CAAAAAGCTT
    TATAAGCCAG AGGTGCAGCT CCGGAGGCCA AACTGGTCCA AGCTTGTGGC TGAGGACCTC
    TCCCAGGACT GCTTCTGGAC AAAGGTGAAG GAGGACCGCT TTGAGAACAA TGAACTTTTC
    GCCAAACTTA CCCTTACCTT CTCTGCCCAG ACCAAGACTT CCAAAGCCAA GAAGGATCAA
    GAAGGTGGAG AAGAAAAGAA ATCTGTGCAA AAGAAAAAAG TAAAAGAGTT AAAGGTGTTG
    GATTCAAAGA CAGCCCAGAA TCTCTCAATC TTTTTGGGTT CCTTCCGCAT GCCCTATCAA
    GAGATTAAGA ATGTCATCCT GGAGGTGAAT GAGGCTGTTC TGACTGAGTC TATGATCCAG
    AACCTCATTA AGCAAATGCC AGAGCCAGAG CAGTTAAAAA TGCTTTCTGA ACTGAAGGAT
    GAATATGATG ACCTGGCTGA GTCAGAGCAG TTTGGCGTGG TGATGGGCAC TGTGCCCCGA
    CTGCGGCCTC GCCTCAATGC CATTCTCTTC AAGCTACAAT TCAGCGAGCA AGTGGAGAAT
    ATCAAGCCAG AGATTGTGTC TGTCACTGCT GCATGTGAGG AGTTACGTAA GAGTGAGAGC
    TTTTCCAATC TCCTAGAGAT TACCTTGCTT GTTGGAAATT ACATGAATGC TGGCTCCAGA
    AATGCTGGTG CTTTTGGCTT CAATATCAGC TTCCTCTGTA AGCTTCGAGA CACCAAGTCC
    ACAGATCAGA AGATGACGTT GTTACACTTC TTGGCTGAGT TGTGTGAGAA TGACTATCCC
    GATGTCCTCA AGTTTCCAGA CGAGCTTGCC CATGTGGAGA AAGCCAGCCG AGTTTCTGCT
    GAAAACTTGC AAAAGAACCT AGATCAGATG AAGAAACAAA TTTCTGATGT GGAACGTGAT
    GTTCAGAATT TCCCAGCTGC CACAGATGAA AAAGACAAGT TTGTTGAAAA AATGACCAGC
    TTTGTGAAGG ATGCACAGGA ACAGTATAAC AAGCTGCGGA TGATGCATTC TAACATGGAG
    ACCCTCTATA AGGAGCTGGG CGAGTACTTC CTCTTTGACC CCAAGAAGTT GTCTGTTGAA
    GAATTTTTCA TGGATCTTCA CAATTTTCGG AATATGTTTT TGGTGAGCAA GGGATACTTG
    CTGACTGAAT CTTTTGTGGG CATTTCTCAC TTTCCCCTGG GTTCCTGTTG GCTGGTAGTA
    GGTATACAAT GCCCATGTGT TCCTTCCTCC TCTTCCCTGC CCCAGCAAGC AGTCAAGGAG
    AACCAGAAGC GGCGGGAGAC AGAAGAAAAG ATGAGGCGAG CAAAACTAGC CAAGGAGAAG
    GCAGAGAAGG AGCGGCTAGA GAAGCAGCAG AAGAGAGAGC AACTCATAGA CATGAATGCA
    GAGGGCGATG AGACAGGTGT GATGGACAGT CTTCTAGAAG CCCTGCAGTC AGGGGCAGCA
    TTCCGACGGA AGAGAGGGCC CCGTCAAGCC AACAGGAAGG CCGGGTGTGC AGTCACATCT
    CTGCTAGCTT CGGAGCTGAC CAAGGATGAT GCCATGGCTG CTGTTCCTGC CAAGGTGTCC
    AAGAACAGTG AGACATTCCC CACAATCCTT GAGGAAGCCA AGGAGTTGGT TGGCCGTGCA
    AGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024310154.1
    CDS759..4664
    Translation

    Target ORF information:

    RefSeq Version XM_024454386.1
    Organism Homo sapiens(human)
    Definition Homo sapiens diaphanous related formin 1 (DIAPH1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024454386.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGAGAGTAC AGAGTCAGTA TGGTAAGTTA GATAGGAGGA AGACTGATTT CTTCTTTTCA 
    TGTCAGAAGA GTCTTGAACT GCTTGTGAAC CAGAGGAACC ATGCCTGTCT GGAGAGATTT
    ACCAGCATGA GAATTAAGAA GGAGAAGGAA AAGCCCAATT CTGCTCATAG AAATTCTTCT
    GCATCATATG GGGATGATCC CACAGCACAG TCATTGCAAG ATGTTTCAGA TGAACAAGTG
    CTGGTTCTCT TTGAACAGAT GCTGCTGGAT ATGAACCTGA ATGAGGAGAA ACAGCAACCT
    TTGAGGGAGA AGGACATCAT CATCAAGAGG GAGATGGTGT CCCAATACTT GTACACCTCC
    AAGGCTGGCA TGAGCCAGAA GGAGAGCTCT AAGTCTGCCA TGATGTATAT TCAGGAGTTG
    AGGTCAGGCT TGCGGGATAT GCCTCTGCTC AGCTGCCTGG AGTCCCTTCG TGTGTCTCTC
    AACAACAACC CTGTCAGTTG GGTGCAAACA TTTGGTGCTG AAGGCTTGGC CTCCTTATTG
    GACATTCTTA AACGACTTCA TGATGAGAAA GAAGAGACTG CTGGGAGTTA CGATAGCCGG
    AACAAGCATG AGATCATTCG CTGCTTGAAA GCTTTTATGA ACAACAAGTT TGGAATCAAG
    ACCATGTTGG AGACAGAAGA AGGAATCCTA CTGCTGGTCA GAGCCATGGA TCCTGCTGTT
    CCCAACATGA TGATTGATGC AGCTAAGCTG CTTTCTGCTC TTTGTATTCT ACCGCAGCCA
    GAGGACATGA ATGAAAGGGT TTTGGAGGCA ATGACAGAAA GAGCTGAGAT GGATGAAGTG
    GAACGTTTCC AGCCGCTGCT GGATGGATTA AAAAGTGGAA CCACTATTGC ACTGAAGGTT
    GGATGCCTAC AGCTGATCAA TGCTCTCATC ACACCAGCGG AGGAACTTGA CTTCCGAGTT
    CACATCAGAA GTGAACTGAT GCGTTTGGGG CTACATCAGG TGTTGCAGGA CCTTCGAGAG
    ATTGAAAATG AAGATATGAG AGTGCAACTA AATGTGTTTG ATGAACAAGG GGAAGAGGAT
    TCCTATGACC TGAAGGGACG GCTGGATGAC ATTCGCATGG AGATGGATGA CTTTAATGAA
    GTCTTTCAGA TTCTCTTAAA CACAGTGAAG GATTCAAAGG CAGAGCCACA CTTCCTTTCC
    ATCCTGCAGC ACTTACTCTT GGTCCGAAAT GACTATGAGG CCAGACCTCA GTACTATAAG
    TTGATTGAAG AATGTATTTC CCAGATAGTT CTGCACAAGA ACGGGGCTGA TCCTGACTTC
    AAGTGCCGGC ACCTCCAGAT TGAGATTGAG GGATTAATTG ATCAAATGAT TGATAAGACA
    AAGGTGGAGA AATCTGAAGC CAAAGCTGCA GAGCTGGAAA AGAAGTTGGA CTCAGAGTTA
    ACAGCCCGAC ATGAGCTACA GGTGGAAATG AAAAAGATGG AAAGTGACTT TGAGCAGAAG
    CTTCAAGATC TTCAGGGAGA AAAAGATGCA CTGCATTCTG AAAAGCAGCA AATTGCCACA
    GAGAAACAGG ACCTGGAAGC AGAGGTGTCC CAGCTCACAG GAGAGGTTGC CAAGCTGACA
    AAGGAACTGG AAGATGCCAA GAAAGAAATG GCTTCCCTCT CTGCGGCAGC TATTACTGTA
    CCTCCTTCTG TTCCTAGTCG TGCTCCTGTT CCCCCTGCCC CTCCTTTACC TGGTGACTCT
    GGCACTATTA TTCCACCACC ACCTGCTCCT GGGGATAGTA CCACTCCTCC TCCTCCTCCT
    CCTCCTCCTC CTCCTCCACC TCCTTTGCCT GGGGGTGTTT GCATCTCCTC ACCCCCTTCT
    TTACCTGGAG GTACTGCTAT CTCTCCACCC CCTCCTTTGT CTGGGGATGC TACCATCCCT
    CCACCCCCTC CTTTGCCTGA GGGTGTTGGC ATCCCTTCAC CCTCTTCTTT GCCTGGAGGT
    ACTGCCATCC CCCCACCTCC TCCTTTGCCT GGGAGTGCTA GAATCCCCCC ACCACCACCT
    CCTTTGCCTG GGAGTGCTGG AATTCCCCCC CCACCTCCTC CCTTGCCTGG AGAAGCAGGA
    ATGCCACCTC CTCCTCCCCC TCTTCCTGGT GGTCCTGGAA TCCCTCCACC TCCTCCATTT
    CCCGGAGGCC CTGGCATTCC TCCACCTCCA CCCGGAATGG GTATGCCTCC ACCTCCCCCA
    TTTGGATTTG GAGTTCCTGC AGCCCCAGTT CTGCCATTTG GATTAACCCC CAAAAAGCTT
    TATAAGCCAG AGGTGCAGCT CCGGAGGCCA AACTGGTCCA AGCTTGTGGC TGAGGACCTC
    TCCCAGGACT GCTTCTGGAC AAAGGTGAAG GAGGACCGCT TTGAGAACAA TGAACTTTTC
    GCCAAACTTA CCCTTACCTT CTCTGCCCAG ACCAAGACTT CCAAAGCCAA GAAGGATCAA
    GAAGGTGGAG AAGAAAAGAA ATCTGTGCAA AAGAAAAAAG TAAAAGAGTT AAAGGTGTTG
    GATTCAAAGA CAGCCCAGAA TCTCTCAATC TTTTTGGGTT CCTTCCGCAT GCCCTATCAA
    GAGATTAAGA ATGTCATCCT GGAGGTGAAT GAGGCTGTTC TGACTGAGTC TATGATCCAG
    AACCTCATTA AGCAAATGCC AGAGCCAGAG CAGTTAAAAA TGCTTTCTGA ACTGAAGGAT
    GAATATGATG ACCTGGCTGA GTCAGAGCAG TTTGGCGTGG TGATGGGCAC TGTGCCCCGA
    CTGCGGCCTC GCCTCAATGC CATTCTCTTC AAGCTACAAT TCAGCGAGCA AGTGGAGAAT
    ATCAAGCCAG AGATTGTGTC TGTCACTGCT GCATGTGAGG AGTTACGTAA GAGTGAGAGC
    TTTTCCAATC TCCTAGAGAT TACCTTGCTT GTTGGAAATT ACATGAATGC TGGCTCCAGA
    AATGCTGGTG CTTTTGGCTT CAATATCAGC TTCCTCTGTA AGCTTCGAGA CACCAAGTCC
    ACAGATCAGA AGATGACGTT GTTACACTTC TTGGCTGAGT TGTGTGAGAA TGACTATCCC
    GATGTCCTCA AGTTTCCAGA CGAGCTTGCC CATGTGGAGA AAGCCAGCCG AGTTTCTGCT
    GAAAACTTGC AAAAGAACCT AGATCAGATG AAGAAACAAA TTTCTGATGT GGAACGTGAT
    GTTCAGAATT TCCCAGCTGC CACAGATGAA AAAGACAAGT TTGTTGAAAA AATGACCAGC
    TTTGTGAAGG ATGCACAGGA ACAGTATAAC AAGCTGCGGA TGATGCATTC TAACATGGAG
    ACCCTCTATA AGGAGCTGGG CGAGTACTTC CTCTTTGACC CCAAGAAGTT GTCTGTTGAA
    GAATTTTTCA TGGATCTTCA CAATTTTCGG AATATGTTTT TGGTGAGCAA GGGATACTTG
    CTGACTGAAT CTTTTGTGGG CATTTCTCAC TTTCCCCTGG GTTCCTGTTG GCTGGTAGTA
    GGTATACAAT GCCCATGTGT TCCTTCCTCC TCTTCCCTGC CCCAGCAAGC AGTCAAGGAG
    AACCAGAAGC GGCGGGAGAC AGAAGAAAAG ATGAGGCGAG CAAAACTAGC CAAGGAGAAG
    GCAGAGAAGG AGCGGCTAGA GAAGCAGCAG AAGAGAGAGC AACTCATAGA CATGAATGCA
    GAGGGCGATG AGACAGGTGT GATGGACAGT CTTCTAGAAG CCCTGCAGTC AGGGGCAGCA
    TTCCGACGGA AGAGAGGGCC CCGTCAAGCC AACAGGAAGG CCGGGTGTGC AGTCACATCT
    CTGCTAGCTT CGGAGCTGAC CAAGGATGAT GCCATGGCTG CTGTTCCTGC CAAGGTGTCC
    AAGAACAGTG AGACATTCCC CACAATCCTT GAGGAAGCCA AGGAGTTGGT TGGCCGTGCA
    AGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu105640
    Clone ID Related Accession (Same CDS sequence) XM_024454387.1
    Accession Version XM_024454387.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3876bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product protein diaphanous homolog 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGTGATAA ACCTCTCCTG TGGGAAGAAA GTTGGACTGC GAGTTCAAGA AACTCTGAAG 
    CGGCTCATGG CAGATGAGCT GGAGAGATTT ACCAGCATGA GAATTAAGAA GGAGAAGGAA
    AAGCCCAATT CTGCTCATAG AAATTCTTCT GCATCATATG GGGATGATCC CACAGCACAG
    TCATTGCAAG ATGTTTCAGA TGAACAAGTG CTGGTTCTCT TTGAACAGAT GCTGCTGGAT
    ATGAACCTGA ATGAGGAGAA ACAGCAACCT TTGAGGGAGA AGGACATCAT CATCAAGAGG
    GAGATGGTGT CCCAATACTT GTACACCTCC AAGGCTGGCA TGAGCCAGAA GGAGAGCTCT
    AAGTCTGCCA TGATGTATAT TCAGGAGTTG AGGTCAGGCT TGCGGGATAT GCCTCTGCTC
    AGCTGCCTGG AGTCCCTTCG TGTGTCTCTC AACAACAACC CTGTCAGTTG GGTGCAAACA
    TTTGGTGCTG AAGGCTTGGC CTCCTTATTG GACATTCTTA AACGACTTCA TGATGAGAAA
    GAAGAGACTG CTGGGAGTTA CGATAGCCGG AACAAGCATG AGATCATTCG CTGCTTGAAA
    GCTTTTATGA ACAACAAGTT TGGAATCAAG ACCATGTTGG AGACAGAAGA AGGAATCCTA
    CTGCTGGTCA GAGCCATGGA TCCTGCTGTT CCCAACATGA TGATTGATGC AGCTAAGCTG
    CTTTCTGCTC TTTGTATTCT ACCGCAGCCA GAGGACATGA ATGAAAGGGT TTTGGAGGCA
    ATGACAGAAA GAGCTGAGAT GGATGAAGTG GAACGTTTCC AGCCGCTGCT GGATGGATTA
    AAAAGTGGAA CCACTATTGC ACTGAAGGTT GGATGCCTAC AGCTGATCAA TGCTCTCATC
    ACACCAGCGG AGGAACTTGA CTTCCGAGTT CACATCAGAA GTGAACTGAT GCGTTTGGGG
    CTACATCAGG TGTTGCAGGA CCTTCGAGAG ATTGAAAATG AAGATATGAG AGTGCAACTA
    AATGTGTTTG ATGAACAAGG GGAAGAGGAT TCCTATGACC TGAAGGGACG GCTGGATGAC
    ATTCGCATGG AGATGGATGA CTTTAATGAA GTCTTTCAGA TTCTCTTAAA CACAGTGAAG
    GATTCAAAGG CAGAGCCACA CTTCCTTTCC ATCCTGCAGC ACTTACTCTT GGTCCGAAAT
    GACTATGAGG CCAGACCTCA GTACTATAAG TTGATTGAAG AATGTATTTC CCAGATAGTT
    CTGCACAAGA ACGGGGCTGA TCCTGACTTC AAGTGCCGGC ACCTCCAGAT TGAGATTGAG
    GGATTAATTG ATCAAATGAT TGATAAGACA AAGGTGGAGA AATCTGAAGC CAAAGCTGCA
    GAGCTGGAAA AGAAGTTGGA CTCAGAGTTA ACAGCCCGAC ATGAGCTACA GGTGGAAATG
    AAAAAGATGG AAAGTGACTT TGAGCAGAAG CTTCAAGATC TTCAGGGAGA AAAAGATGCA
    CTGCATTCTG AAAAGCAGCA AATTGCCACA GAGAAACAGG ACCTGGAAGC AGAGGTGTCC
    CAGCTCACAG GAGAGGTTGC CAAGCTGACA AAGGAACTGG AAGATGCCAA GAAAGAAATG
    GCTTCCCTCT CTGCGGCAGC TATTACTGTA CCTCCTTCTG TTCCTAGTCG TGCTCCTGTT
    CCCCCTGCCC CTCCTTTACC TGGTGACTCT GGCACTATTA TTCCACCACC ACCTGCTCCT
    GGGGATAGTA CCACTCCTCC TCCTCCTCCT CCTCCTCCTC CTCCTCCACC TCCTTTGCCT
    GGGGGTGTTT GCATCTCCTC ACCCCCTTCT TTACCTGGAG GTACTGCTAT CTCTCCACCC
    CCTCCTTTGT CTGGGGATGC TACCATCCCT CCACCCCCTC CTTTGCCTGA GGGTGTTGGC
    ATCCCTTCAC CCTCTTCTTT GCCTGGAGGT ACTGCCATCC CCCCACCTCC TCCTTTGCCT
    GGGAGTGCTA GAATCCCCCC ACCACCACCT CCTTTGCCTG GGAGTGCTGG AATTCCCCCC
    CCACCTCCTC CCTTGCCTGG AGAAGCAGGA ATGCCACCTC CTCCTCCCCC TCTTCCTGGT
    GGTCCTGGAA TCCCTCCACC TCCTCCATTT CCCGGAGGCC CTGGCATTCC TCCACCTCCA
    CCCGGAATGG GTATGCCTCC ACCTCCCCCA TTTGGATTTG GAGTTCCTGC AGCCCCAGTT
    CTGCCATTTG GATTAACCCC CAAAAAGCTT TATAAGCCAG AGGTGCAGCT CCGGAGGCCA
    AACTGGTCCA AGCTTGTGGC TGAGGACCTC TCCCAGGACT GCTTCTGGAC AAAGGTGAAG
    GAGGACCGCT TTGAGAACAA TGAACTTTTC GCCAAACTTA CCCTTACCTT CTCTGCCCAG
    ACCAAGACTT CCAAAGCCAA GAAGGATCAA GAAGGTGGAG AAGAAAAGAA ATCTGTGCAA
    AAGAAAAAAG TAAAAGAGTT AAAGGTGTTG GATTCAAAGA CAGCCCAGAA TCTCTCAATC
    TTTTTGGGTT CCTTCCGCAT GCCCTATCAA GAGATTAAGA ATGTCATCCT GGAGGTGAAT
    GAGGCTGTTC TGACTGAGTC TATGATCCAG AACCTCATTA AGCAAATGCC AGAGCCAGAG
    CAGTTAAAAA TGCTTTCTGA ACTGAAGGAT GAATATGATG ACCTGGCTGA GTCAGAGCAG
    TTTGGCGTGG TGATGGGCAC TGTGCCCCGA CTGCGGCCTC GCCTCAATGC CATTCTCTTC
    AAGCTACAAT TCAGCGAGCA AGTGGAGAAT ATCAAGCCAG AGATTGTGTC TGTCACTGCT
    GCATGTGAGG AGTTACGTAA GAGTGAGAGC TTTTCCAATC TCCTAGAGAT TACCTTGCTT
    GTTGGAAATT ACATGAATGC TGGCTCCAGA AATGCTGGTG CTTTTGGCTT CAATATCAGC
    TTCCTCTGTA AGCTTCGAGA CACCAAGTCC ACAGATCAGA AGATGACGTT GTTACACTTC
    TTGGCTGAGT TGTGTGAGAA TGACTATCCC GATGTCCTCA AGTTTCCAGA CGAGCTTGCC
    CATGTGGAGA AAGCCAGCCG AGTTTCTGCT GAAAACTTGC AAAAGAACCT AGATCAGATG
    AAGAAACAAA TTTCTGATGT GGAACGTGAT GTTCAGAATT TCCCAGCTGC CACAGATGAA
    AAAGACAAGT TTGTTGAAAA AATGACCAGC TTTGTGAAGG ATGCACAGGA ACAGTATAAC
    AAGCTGCGGA TGATGCATTC TAACATGGAG ACCCTCTATA AGGAGCTGGG CGAGTACTTC
    CTCTTTGACC CCAAGAAGTT GTCTGTTGAA GAATTTTTCA TGGATCTTCA CAATTTTCGG
    AATATGTTTT TGGTGAGCAA GGGATACTTG CTGACTGAAT CTTTTGTGGG CATTTCTCAC
    TTTCCCCTGG GTTCCTGTTG GCTGGTAGTA GGTATACAAT GCCCATGTGT TCCTTCCTCC
    TCTTCCCTGC CCCAGCAAGC AGTCAAGGAG AACCAGAAGC GGCGGGAGAC AGAAGAAAAG
    ATGAGGCGAG CAAAACTAGC CAAGGAGAAG GCAGAGAAGG AGCGGCTAGA GAAGCAGCAG
    AAGAGAGAGC AACTCATAGA CATGAATGCA GAGGGCGATG AGACAGGTGT GATGGACAGT
    CTTCTAGAAG CCCTGCAGTC AGGGGCAGCA TTCCGACGGA AGAGAGGGCC CCGTCAAGCC
    AACAGGAAGG CCGGGTGTGC AGTCACATCT CTGCTAGCTT CGGAGCTGAC CAAGGATGAT
    GCCATGGCTG CTGTTCCTGC CAAGGTGTCC AAGAACAGTG AGACATTCCC CACAATCCTT
    GAGGAAGCCA AGGAGTTGGT TGGCCGTGCA AGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024310155.1
    CDS16..3891
    Translation

    Target ORF information:

    RefSeq Version XM_024454387.1
    Organism Homo sapiens(human)
    Definition Homo sapiens diaphanous related formin 1 (DIAPH1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024454387.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGTGATAA ACCTCTCCTG TGGGAAGAAA GTTGGACTGC GAGTTCAAGA AACTCTGAAG 
    CGGCTCATGG CAGATGAGCT GGAGAGATTT ACCAGCATGA GAATTAAGAA GGAGAAGGAA
    AAGCCCAATT CTGCTCATAG AAATTCTTCT GCATCATATG GGGATGATCC CACAGCACAG
    TCATTGCAAG ATGTTTCAGA TGAACAAGTG CTGGTTCTCT TTGAACAGAT GCTGCTGGAT
    ATGAACCTGA ATGAGGAGAA ACAGCAACCT TTGAGGGAGA AGGACATCAT CATCAAGAGG
    GAGATGGTGT CCCAATACTT GTACACCTCC AAGGCTGGCA TGAGCCAGAA GGAGAGCTCT
    AAGTCTGCCA TGATGTATAT TCAGGAGTTG AGGTCAGGCT TGCGGGATAT GCCTCTGCTC
    AGCTGCCTGG AGTCCCTTCG TGTGTCTCTC AACAACAACC CTGTCAGTTG GGTGCAAACA
    TTTGGTGCTG AAGGCTTGGC CTCCTTATTG GACATTCTTA AACGACTTCA TGATGAGAAA
    GAAGAGACTG CTGGGAGTTA CGATAGCCGG AACAAGCATG AGATCATTCG CTGCTTGAAA
    GCTTTTATGA ACAACAAGTT TGGAATCAAG ACCATGTTGG AGACAGAAGA AGGAATCCTA
    CTGCTGGTCA GAGCCATGGA TCCTGCTGTT CCCAACATGA TGATTGATGC AGCTAAGCTG
    CTTTCTGCTC TTTGTATTCT ACCGCAGCCA GAGGACATGA ATGAAAGGGT TTTGGAGGCA
    ATGACAGAAA GAGCTGAGAT GGATGAAGTG GAACGTTTCC AGCCGCTGCT GGATGGATTA
    AAAAGTGGAA CCACTATTGC ACTGAAGGTT GGATGCCTAC AGCTGATCAA TGCTCTCATC
    ACACCAGCGG AGGAACTTGA CTTCCGAGTT CACATCAGAA GTGAACTGAT GCGTTTGGGG
    CTACATCAGG TGTTGCAGGA CCTTCGAGAG ATTGAAAATG AAGATATGAG AGTGCAACTA
    AATGTGTTTG ATGAACAAGG GGAAGAGGAT TCCTATGACC TGAAGGGACG GCTGGATGAC
    ATTCGCATGG AGATGGATGA CTTTAATGAA GTCTTTCAGA TTCTCTTAAA CACAGTGAAG
    GATTCAAAGG CAGAGCCACA CTTCCTTTCC ATCCTGCAGC ACTTACTCTT GGTCCGAAAT
    GACTATGAGG CCAGACCTCA GTACTATAAG TTGATTGAAG AATGTATTTC CCAGATAGTT
    CTGCACAAGA ACGGGGCTGA TCCTGACTTC AAGTGCCGGC ACCTCCAGAT TGAGATTGAG
    GGATTAATTG ATCAAATGAT TGATAAGACA AAGGTGGAGA AATCTGAAGC CAAAGCTGCA
    GAGCTGGAAA AGAAGTTGGA CTCAGAGTTA ACAGCCCGAC ATGAGCTACA GGTGGAAATG
    AAAAAGATGG AAAGTGACTT TGAGCAGAAG CTTCAAGATC TTCAGGGAGA AAAAGATGCA
    CTGCATTCTG AAAAGCAGCA AATTGCCACA GAGAAACAGG ACCTGGAAGC AGAGGTGTCC
    CAGCTCACAG GAGAGGTTGC CAAGCTGACA AAGGAACTGG AAGATGCCAA GAAAGAAATG
    GCTTCCCTCT CTGCGGCAGC TATTACTGTA CCTCCTTCTG TTCCTAGTCG TGCTCCTGTT
    CCCCCTGCCC CTCCTTTACC TGGTGACTCT GGCACTATTA TTCCACCACC ACCTGCTCCT
    GGGGATAGTA CCACTCCTCC TCCTCCTCCT CCTCCTCCTC CTCCTCCACC TCCTTTGCCT
    GGGGGTGTTT GCATCTCCTC ACCCCCTTCT TTACCTGGAG GTACTGCTAT CTCTCCACCC
    CCTCCTTTGT CTGGGGATGC TACCATCCCT CCACCCCCTC CTTTGCCTGA GGGTGTTGGC
    ATCCCTTCAC CCTCTTCTTT GCCTGGAGGT ACTGCCATCC CCCCACCTCC TCCTTTGCCT
    GGGAGTGCTA GAATCCCCCC ACCACCACCT CCTTTGCCTG GGAGTGCTGG AATTCCCCCC
    CCACCTCCTC CCTTGCCTGG AGAAGCAGGA ATGCCACCTC CTCCTCCCCC TCTTCCTGGT
    GGTCCTGGAA TCCCTCCACC TCCTCCATTT CCCGGAGGCC CTGGCATTCC TCCACCTCCA
    CCCGGAATGG GTATGCCTCC ACCTCCCCCA TTTGGATTTG GAGTTCCTGC AGCCCCAGTT
    CTGCCATTTG GATTAACCCC CAAAAAGCTT TATAAGCCAG AGGTGCAGCT CCGGAGGCCA
    AACTGGTCCA AGCTTGTGGC TGAGGACCTC TCCCAGGACT GCTTCTGGAC AAAGGTGAAG
    GAGGACCGCT TTGAGAACAA TGAACTTTTC GCCAAACTTA CCCTTACCTT CTCTGCCCAG
    ACCAAGACTT CCAAAGCCAA GAAGGATCAA GAAGGTGGAG AAGAAAAGAA ATCTGTGCAA
    AAGAAAAAAG TAAAAGAGTT AAAGGTGTTG GATTCAAAGA CAGCCCAGAA TCTCTCAATC
    TTTTTGGGTT CCTTCCGCAT GCCCTATCAA GAGATTAAGA ATGTCATCCT GGAGGTGAAT
    GAGGCTGTTC TGACTGAGTC TATGATCCAG AACCTCATTA AGCAAATGCC AGAGCCAGAG
    CAGTTAAAAA TGCTTTCTGA ACTGAAGGAT GAATATGATG ACCTGGCTGA GTCAGAGCAG
    TTTGGCGTGG TGATGGGCAC TGTGCCCCGA CTGCGGCCTC GCCTCAATGC CATTCTCTTC
    AAGCTACAAT TCAGCGAGCA AGTGGAGAAT ATCAAGCCAG AGATTGTGTC TGTCACTGCT
    GCATGTGAGG AGTTACGTAA GAGTGAGAGC TTTTCCAATC TCCTAGAGAT TACCTTGCTT
    GTTGGAAATT ACATGAATGC TGGCTCCAGA AATGCTGGTG CTTTTGGCTT CAATATCAGC
    TTCCTCTGTA AGCTTCGAGA CACCAAGTCC ACAGATCAGA AGATGACGTT GTTACACTTC
    TTGGCTGAGT TGTGTGAGAA TGACTATCCC GATGTCCTCA AGTTTCCAGA CGAGCTTGCC
    CATGTGGAGA AAGCCAGCCG AGTTTCTGCT GAAAACTTGC AAAAGAACCT AGATCAGATG
    AAGAAACAAA TTTCTGATGT GGAACGTGAT GTTCAGAATT TCCCAGCTGC CACAGATGAA
    AAAGACAAGT TTGTTGAAAA AATGACCAGC TTTGTGAAGG ATGCACAGGA ACAGTATAAC
    AAGCTGCGGA TGATGCATTC TAACATGGAG ACCCTCTATA AGGAGCTGGG CGAGTACTTC
    CTCTTTGACC CCAAGAAGTT GTCTGTTGAA GAATTTTTCA TGGATCTTCA CAATTTTCGG
    AATATGTTTT TGGTGAGCAA GGGATACTTG CTGACTGAAT CTTTTGTGGG CATTTCTCAC
    TTTCCCCTGG GTTCCTGTTG GCTGGTAGTA GGTATACAAT GCCCATGTGT TCCTTCCTCC
    TCTTCCCTGC CCCAGCAAGC AGTCAAGGAG AACCAGAAGC GGCGGGAGAC AGAAGAAAAG
    ATGAGGCGAG CAAAACTAGC CAAGGAGAAG GCAGAGAAGG AGCGGCTAGA GAAGCAGCAG
    AAGAGAGAGC AACTCATAGA CATGAATGCA GAGGGCGATG AGACAGGTGT GATGGACAGT
    CTTCTAGAAG CCCTGCAGTC AGGGGCAGCA TTCCGACGGA AGAGAGGGCC CCGTCAAGCC
    AACAGGAAGG CCGGGTGTGC AGTCACATCT CTGCTAGCTT CGGAGCTGAC CAAGGATGAT
    GCCATGGCTG CTGTTCCTGC CAAGGTGTCC AAGAACAGTG AGACATTCCC CACAATCCTT
    GAGGAAGCCA AGGAGTTGGT TGGCCGTGCA AGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu26133
    Clone ID Related Accession (Same CDS sequence) NM_001079812.2 , NM_001079812.3
    Accession Version NM_001079812.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3792bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-19
    Organism Homo sapiens(human)
    Product protein diaphanous homolog 1 isoform 2
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA593734.1, BC117257.1, AK023345.1 and AY007129.1. This sequence is a reference standard in the RefSeqGene project. On May 24, 2008 this sequence version replaced NM_001079812.1. Transcript Variant: This variant (2) lacks an in-frame exon in the 5' coding region, as compared to variant 1, which results in a shorter isoform (2). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: DQ067453.1, BC117257.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGAGCCGC CCGGCGGGAG CCTGGGGCCC GGCCGCGGGA CCCGGGACAA GAAGAAGGGC 
    CGGAGCCCAG ATGAGCTGCC CTCGGCGGGC GGCGACGGCG GCAAATCTAA GAAATTTCTG
    GAGAGATTTA CCAGCATGAG AATTAAGAAG GAGAAGGAAA AGCCCAATTC TGCTCATAGA
    AATTCTTCTG CATCATATGG GGATGATCCC ACAGCACAGT CATTGCAAGA TGTTTCAGAT
    GAACAAGTGC TGGTTCTCTT TGAACAGATG CTGCTGGATA TGAACCTGAA TGAGGAGAAA
    CAGCAACCTT TGAGGGAGAA GGACATCATC ATCAAGAGGG AGATGGTGTC CCAATACTTG
    TACACCTCCA AGGCTGGCAT GAGCCAGAAG GAGAGCTCTA AGTCTGCCAT GATGTATATT
    CAGGAGTTGA GGTCAGGCTT GCGGGATATG CCTCTGCTCA GCTGCCTGGA GTCCCTTCGT
    GTGTCTCTCA ACAACAACCC TGTCAGTTGG GTGCAAACAT TTGGTGCTGA AGGCTTGGCC
    TCCTTATTGG ACATTCTTAA ACGACTTCAT GATGAGAAAG AAGAGACTGC TGGGAGTTAC
    GATAGCCGGA ACAAGCATGA GATCATTCGC TGCTTGAAAG CTTTTATGAA CAACAAGTTT
    GGAATCAAGA CCATGTTGGA GACAGAAGAA GGAATCCTAC TGCTGGTCAG AGCCATGGAT
    CCTGCTGTTC CCAACATGAT GATTGATGCA GCTAAGCTGC TTTCTGCTCT TTGTATTCTA
    CCGCAGCCAG AGGACATGAA TGAAAGGGTT TTGGAGGCAA TGACAGAAAG AGCTGAGATG
    GATGAAGTGG AACGTTTCCA GCCGCTGCTG GATGGATTAA AAAGTGGAAC CACTATTGCA
    CTGAAGGTTG GATGCCTACA GCTGATCAAT GCTCTCATCA CACCAGCGGA GGAACTTGAC
    TTCCGAGTTC ACATCAGAAG TGAACTGATG CGTTTGGGGC TACATCAGGT GTTGCAGGAC
    CTTCGAGAGA TTGAAAATGA AGATATGAGA GTGCAACTAA ATGTGTTTGA TGAACAAGGG
    GAAGAGGATT CCTATGACCT GAAGGGACGG CTGGATGACA TTCGCATGGA GATGGATGAC
    TTTAATGAAG TCTTTCAGAT TCTCTTAAAC ACAGTGAAGG ATTCAAAGGC AGAGCCACAC
    TTCCTTTCCA TCCTGCAGCA CTTACTCTTG GTCCGAAATG ACTATGAGGC CAGACCTCAG
    TACTATAAGT TGATTGAAGA ATGTATTTCC CAGATAGTTC TGCACAAGAA CGGGGCTGAT
    CCTGACTTCA AGTGCCGGCA CCTCCAGATT GAGATTGAGG GATTAATTGA TCAAATGATT
    GATAAGACAA AGGTGGAGAA ATCTGAAGCC AAAGCTGCAG AGCTGGAAAA GAAGTTGGAC
    TCAGAGTTAA CAGCCCGACA TGAGCTACAG GTGGAAATGA AAAAGATGGA AAGTGACTTT
    GAGCAGAAGC TTCAAGATCT TCAGGGAGAA AAAGATGCAC TGCATTCTGA AAAGCAGCAA
    ATTGCCACAG AGAAACAGGA CCTGGAAGCA GAGGTGTCCC AGCTCACAGG AGAGGTTGCC
    AAGCTGACAA AGGAACTGGA AGATGCCAAG AAAGAAATGG CTTCCCTCTC TGCGGCAGCT
    ATTACTGTAC CTCCTTCTGT TCCTAGTCGT GCTCCTGTTC CCCCTGCCCC TCCTTTACCT
    GGTGACTCTG GCACTATTAT TCCACCACCA CCTGCTCCTG GGGATAGTAC CACTCCTCCT
    CCTCCTCCTC CTCCTCCTCC TCCTCCACCT CCTTTGCCTG GGGGTGTTTG CATCTCCTCA
    CCCCCTTCTT TACCTGGAGG TACTGCTATC TCTCCACCCC CTCCTTTGTC TGGGGATGCT
    ACCATCCCTC CACCCCCTCC TTTGCCTGAG GGTGTTGGCA TCCCTTCACC CTCTTCTTTG
    CCTGGAGGTA CTGCCATCCC CCCACCTCCT CCTTTGCCTG GGAGTGCTAG AATCCCCCCA
    CCACCACCTC CTTTGCCTGG GAGTGCTGGA ATTCCCCCCC CACCTCCTCC CTTGCCTGGA
    GAAGCAGGAA TGCCACCTCC TCCTCCCCCT CTTCCTGGTG GTCCTGGAAT CCCTCCACCT
    CCTCCATTTC CCGGAGGCCC TGGCATTCCT CCACCTCCAC CCGGAATGGG TATGCCTCCA
    CCTCCCCCAT TTGGATTTGG AGTTCCTGCA GCCCCAGTTC TGCCATTTGG ATTAACCCCC
    AAAAAGCTTT ATAAGCCAGA GGTGCAGCTC CGGAGGCCAA ACTGGTCCAA GCTTGTGGCT
    GAGGACCTCT CCCAGGACTG CTTCTGGACA AAGGTGAAGG AGGACCGCTT TGAGAACAAT
    GAACTTTTCG CCAAACTTAC CCTTACCTTC TCTGCCCAGA CCAAGACTTC CAAAGCCAAG
    AAGGATCAAG AAGGTGGAGA AGAAAAGAAA TCTGTGCAAA AGAAAAAAGT AAAAGAGTTA
    AAGGTGTTGG ATTCAAAGAC AGCCCAGAAT CTCTCAATCT TTTTGGGTTC CTTCCGCATG
    CCCTATCAAG AGATTAAGAA TGTCATCCTG GAGGTGAATG AGGCTGTTCT GACTGAGTCT
    ATGATCCAGA ACCTCATTAA GCAAATGCCA GAGCCAGAGC AGTTAAAAAT GCTTTCTGAA
    CTGAAGGATG AATATGATGA CCTGGCTGAG TCAGAGCAGT TTGGCGTGGT GATGGGCACT
    GTGCCCCGAC TGCGGCCTCG CCTCAATGCC ATTCTCTTCA AGCTACAATT CAGCGAGCAA
    GTGGAGAATA TCAAGCCAGA GATTGTGTCT GTCACTGCTG CATGTGAGGA GTTACGTAAG
    AGTGAGAGCT TTTCCAATCT CCTAGAGATT ACCTTGCTTG TTGGAAATTA CATGAATGCT
    GGCTCCAGAA ATGCTGGTGC TTTTGGCTTC AATATCAGCT TCCTCTGTAA GCTTCGAGAC
    ACCAAGTCCA CAGATCAGAA GATGACGTTG TTACACTTCT TGGCTGAGTT GTGTGAGAAT
    GACTATCCCG ATGTCCTCAA GTTTCCAGAC GAGCTTGCCC ATGTGGAGAA AGCCAGCCGA
    GTTTCTGCTG AAAACTTGCA AAAGAACCTA GATCAGATGA AGAAACAAAT TTCTGATGTG
    GAACGTGATG TTCAGAATTT CCCAGCTGCC ACAGATGAAA AAGACAAGTT TGTTGAAAAA
    ATGACCAGCT TTGTGAAGGA TGCACAGGAA CAGTATAACA AGCTGCGGAT GATGCATTCT
    AACATGGAGA CCCTCTATAA GGAGCTGGGC GAGTACTTCC TCTTTGACCC CAAGAAGTTG
    TCTGTTGAAG AATTTTTCAT GGATCTTCAC AATTTTCGGA ATATGTTTTT GCAAGCAGTC
    AAGGAGAACC AGAAGCGGCG GGAGACAGAA GAAAAGATGA GGCGAGCAAA ACTAGCCAAG
    GAGAAGGCAG AGAAGGAGCG GCTAGAGAAG CAGCAGAAGA GAGAGCAACT CATAGACATG
    AATGCAGAGG GCGATGAGAC AGGTGTGATG GACAGTCTTC TAGAAGCCCT GCAGTCAGGG
    GCAGCATTCC GACGGAAGAG AGGGCCCCGT CAAGCCAACA GGAAGGCCGG GTGTGCAGTC
    ACATCTCTGC TAGCTTCGGA GCTGACCAAG GATGATGCCA TGGCTGCTGT TCCTGCCAAG
    GTGTCCAAGA ACAGTGAGAC ATTCCCCACA ATCCTTGAGG AAGCCAAGGA GTTGGTTGGC
    CGTGCAAGCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001073280.1
    CDS142..3933
    Translation

    Target ORF information:

    RefSeq Version NM_001079812.2
    Organism Homo sapiens(human)
    Definition Homo sapiens diaphanous related formin 1 (DIAPH1), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001079812.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGAGCCGC CCGGCGGGAG CCTGGGGCCC GGCCGCGGGA CCCGGGACAA GAAGAAGGGC 
    CGGAGCCCAG ATGAGCTGCC CTCGGCGGGC GGCGACGGCG GCAAATCTAA GAAATTTCTG
    GAGAGATTTA CCAGCATGAG AATTAAGAAG GAGAAGGAAA AGCCCAATTC TGCTCATAGA
    AATTCTTCTG CATCATATGG GGATGATCCC ACAGCACAGT CATTGCAAGA TGTTTCAGAT
    GAACAAGTGC TGGTTCTCTT TGAACAGATG CTGCTGGATA TGAACCTGAA TGAGGAGAAA
    CAGCAACCTT TGAGGGAGAA GGACATCATC ATCAAGAGGG AGATGGTGTC CCAATACTTG
    TACACCTCCA AGGCTGGCAT GAGCCAGAAG GAGAGCTCTA AGTCTGCCAT GATGTATATT
    CAGGAGTTGA GGTCAGGCTT GCGGGATATG CCTCTGCTCA GCTGCCTGGA GTCCCTTCGT
    GTGTCTCTCA ACAACAACCC TGTCAGTTGG GTGCAAACAT TTGGTGCTGA AGGCTTGGCC
    TCCTTATTGG ACATTCTTAA ACGACTTCAT GATGAGAAAG AAGAGACTGC TGGGAGTTAC
    GATAGCCGGA ACAAGCATGA GATCATTCGC TGCTTGAAAG CTTTTATGAA CAACAAGTTT
    GGAATCAAGA CCATGTTGGA GACAGAAGAA GGAATCCTAC TGCTGGTCAG AGCCATGGAT
    CCTGCTGTTC CCAACATGAT GATTGATGCA GCTAAGCTGC TTTCTGCTCT TTGTATTCTA
    CCGCAGCCAG AGGACATGAA TGAAAGGGTT TTGGAGGCAA TGACAGAAAG AGCTGAGATG
    GATGAAGTGG AACGTTTCCA GCCGCTGCTG GATGGATTAA AAAGTGGAAC CACTATTGCA
    CTGAAGGTTG GATGCCTACA GCTGATCAAT GCTCTCATCA CACCAGCGGA GGAACTTGAC
    TTCCGAGTTC ACATCAGAAG TGAACTGATG CGTTTGGGGC TACATCAGGT GTTGCAGGAC
    CTTCGAGAGA TTGAAAATGA AGATATGAGA GTGCAACTAA ATGTGTTTGA TGAACAAGGG
    GAAGAGGATT CCTATGACCT GAAGGGACGG CTGGATGACA TTCGCATGGA GATGGATGAC
    TTTAATGAAG TCTTTCAGAT TCTCTTAAAC ACAGTGAAGG ATTCAAAGGC AGAGCCACAC
    TTCCTTTCCA TCCTGCAGCA CTTACTCTTG GTCCGAAATG ACTATGAGGC CAGACCTCAG
    TACTATAAGT TGATTGAAGA ATGTATTTCC CAGATAGTTC TGCACAAGAA CGGGGCTGAT
    CCTGACTTCA AGTGCCGGCA CCTCCAGATT GAGATTGAGG GATTAATTGA TCAAATGATT
    GATAAGACAA AGGTGGAGAA ATCTGAAGCC AAAGCTGCAG AGCTGGAAAA GAAGTTGGAC
    TCAGAGTTAA CAGCCCGACA TGAGCTACAG GTGGAAATGA AAAAGATGGA AAGTGACTTT
    GAGCAGAAGC TTCAAGATCT TCAGGGAGAA AAAGATGCAC TGCATTCTGA AAAGCAGCAA
    ATTGCCACAG AGAAACAGGA CCTGGAAGCA GAGGTGTCCC AGCTCACAGG AGAGGTTGCC
    AAGCTGACAA AGGAACTGGA AGATGCCAAG AAAGAAATGG CTTCCCTCTC TGCGGCAGCT
    ATTACTGTAC CTCCTTCTGT TCCTAGTCGT GCTCCTGTTC CCCCTGCCCC TCCTTTACCT
    GGTGACTCTG GCACTATTAT TCCACCACCA CCTGCTCCTG GGGATAGTAC CACTCCTCCT
    CCTCCTCCTC CTCCTCCTCC TCCTCCACCT CCTTTGCCTG GGGGTGTTTG CATCTCCTCA
    CCCCCTTCTT TACCTGGAGG TACTGCTATC TCTCCACCCC CTCCTTTGTC TGGGGATGCT
    ACCATCCCTC CACCCCCTCC TTTGCCTGAG GGTGTTGGCA TCCCTTCACC CTCTTCTTTG
    CCTGGAGGTA CTGCCATCCC CCCACCTCCT CCTTTGCCTG GGAGTGCTAG AATCCCCCCA
    CCACCACCTC CTTTGCCTGG GAGTGCTGGA ATTCCCCCCC CACCTCCTCC CTTGCCTGGA
    GAAGCAGGAA TGCCACCTCC TCCTCCCCCT CTTCCTGGTG GTCCTGGAAT CCCTCCACCT
    CCTCCATTTC CCGGAGGCCC TGGCATTCCT CCACCTCCAC CCGGAATGGG TATGCCTCCA
    CCTCCCCCAT TTGGATTTGG AGTTCCTGCA GCCCCAGTTC TGCCATTTGG ATTAACCCCC
    AAAAAGCTTT ATAAGCCAGA GGTGCAGCTC CGGAGGCCAA ACTGGTCCAA GCTTGTGGCT
    GAGGACCTCT CCCAGGACTG CTTCTGGACA AAGGTGAAGG AGGACCGCTT TGAGAACAAT
    GAACTTTTCG CCAAACTTAC CCTTACCTTC TCTGCCCAGA CCAAGACTTC CAAAGCCAAG
    AAGGATCAAG AAGGTGGAGA AGAAAAGAAA TCTGTGCAAA AGAAAAAAGT AAAAGAGTTA
    AAGGTGTTGG ATTCAAAGAC AGCCCAGAAT CTCTCAATCT TTTTGGGTTC CTTCCGCATG
    CCCTATCAAG AGATTAAGAA TGTCATCCTG GAGGTGAATG AGGCTGTTCT GACTGAGTCT
    ATGATCCAGA ACCTCATTAA GCAAATGCCA GAGCCAGAGC AGTTAAAAAT GCTTTCTGAA
    CTGAAGGATG AATATGATGA CCTGGCTGAG TCAGAGCAGT TTGGCGTGGT GATGGGCACT
    GTGCCCCGAC TGCGGCCTCG CCTCAATGCC ATTCTCTTCA AGCTACAATT CAGCGAGCAA
    GTGGAGAATA TCAAGCCAGA GATTGTGTCT GTCACTGCTG CATGTGAGGA GTTACGTAAG
    AGTGAGAGCT TTTCCAATCT CCTAGAGATT ACCTTGCTTG TTGGAAATTA CATGAATGCT
    GGCTCCAGAA ATGCTGGTGC TTTTGGCTTC AATATCAGCT TCCTCTGTAA GCTTCGAGAC
    ACCAAGTCCA CAGATCAGAA GATGACGTTG TTACACTTCT TGGCTGAGTT GTGTGAGAAT
    GACTATCCCG ATGTCCTCAA GTTTCCAGAC GAGCTTGCCC ATGTGGAGAA AGCCAGCCGA
    GTTTCTGCTG AAAACTTGCA AAAGAACCTA GATCAGATGA AGAAACAAAT TTCTGATGTG
    GAACGTGATG TTCAGAATTT CCCAGCTGCC ACAGATGAAA AAGACAAGTT TGTTGAAAAA
    ATGACCAGCT TTGTGAAGGA TGCACAGGAA CAGTATAACA AGCTGCGGAT GATGCATTCT
    AACATGGAGA CCCTCTATAA GGAGCTGGGC GAGTACTTCC TCTTTGACCC CAAGAAGTTG
    TCTGTTGAAG AATTTTTCAT GGATCTTCAC AATTTTCGGA ATATGTTTTT GCAAGCAGTC
    AAGGAGAACC AGAAGCGGCG GGAGACAGAA GAAAAGATGA GGCGAGCAAA ACTAGCCAAG
    GAGAAGGCAG AGAAGGAGCG GCTAGAGAAG CAGCAGAAGA GAGAGCAACT CATAGACATG
    AATGCAGAGG GCGATGAGAC AGGTGTGATG GACAGTCTTC TAGAAGCCCT GCAGTCAGGG
    GCAGCATTCC GACGGAAGAG AGGGCCCCGT CAAGCCAACA GGAAGGCCGG GTGTGCAGTC
    ACATCTCTGC TAGCTTCGGA GCTGACCAAG GATGATGCCA TGGCTGCTGT TCCTGCCAAG
    GTGTCCAAGA ACAGTGAGAC ATTCCCCACA ATCCTTGAGG AAGCCAAGGA GTTGGTTGGC
    CGTGCAAGCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu26133
    Clone ID Related Accession (Same CDS sequence) NM_001079812.2 , NM_001079812.3
    Accession Version NM_001079812.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3792bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-09
    Organism Homo sapiens(human)
    Product protein diaphanous homolog 1 isoform 2
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA593734.1, BC117257.1, AK023345.1 and AY007129.1. This sequence is a reference standard in the RefSeqGene project. On May 31, 2019 this sequence version replaced NM_001079812.2. Transcript Variant: This variant (2) lacks an in-frame exon in the 5' coding region, as compared to variant 1, which results in a shorter isoform (2). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR7346977.2479246.1, SRR7346977.2276930.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGAGCCGC CCGGCGGGAG CCTGGGGCCC GGCCGCGGGA CCCGGGACAA GAAGAAGGGC 
    CGGAGCCCAG ATGAGCTGCC CTCGGCGGGC GGCGACGGCG GCAAATCTAA GAAATTTCTG
    GAGAGATTTA CCAGCATGAG AATTAAGAAG GAGAAGGAAA AGCCCAATTC TGCTCATAGA
    AATTCTTCTG CATCATATGG GGATGATCCC ACAGCACAGT CATTGCAAGA TGTTTCAGAT
    GAACAAGTGC TGGTTCTCTT TGAACAGATG CTGCTGGATA TGAACCTGAA TGAGGAGAAA
    CAGCAACCTT TGAGGGAGAA GGACATCATC ATCAAGAGGG AGATGGTGTC CCAATACTTG
    TACACCTCCA AGGCTGGCAT GAGCCAGAAG GAGAGCTCTA AGTCTGCCAT GATGTATATT
    CAGGAGTTGA GGTCAGGCTT GCGGGATATG CCTCTGCTCA GCTGCCTGGA GTCCCTTCGT
    GTGTCTCTCA ACAACAACCC TGTCAGTTGG GTGCAAACAT TTGGTGCTGA AGGCTTGGCC
    TCCTTATTGG ACATTCTTAA ACGACTTCAT GATGAGAAAG AAGAGACTGC TGGGAGTTAC
    GATAGCCGGA ACAAGCATGA GATCATTCGC TGCTTGAAAG CTTTTATGAA CAACAAGTTT
    GGAATCAAGA CCATGTTGGA GACAGAAGAA GGAATCCTAC TGCTGGTCAG AGCCATGGAT
    CCTGCTGTTC CCAACATGAT GATTGATGCA GCTAAGCTGC TTTCTGCTCT TTGTATTCTA
    CCGCAGCCAG AGGACATGAA TGAAAGGGTT TTGGAGGCAA TGACAGAAAG AGCTGAGATG
    GATGAAGTGG AACGTTTCCA GCCGCTGCTG GATGGATTAA AAAGTGGAAC CACTATTGCA
    CTGAAGGTTG GATGCCTACA GCTGATCAAT GCTCTCATCA CACCAGCGGA GGAACTTGAC
    TTCCGAGTTC ACATCAGAAG TGAACTGATG CGTTTGGGGC TACATCAGGT GTTGCAGGAC
    CTTCGAGAGA TTGAAAATGA AGATATGAGA GTGCAACTAA ATGTGTTTGA TGAACAAGGG
    GAAGAGGATT CCTATGACCT GAAGGGACGG CTGGATGACA TTCGCATGGA GATGGATGAC
    TTTAATGAAG TCTTTCAGAT TCTCTTAAAC ACAGTGAAGG ATTCAAAGGC AGAGCCACAC
    TTCCTTTCCA TCCTGCAGCA CTTACTCTTG GTCCGAAATG ACTATGAGGC CAGACCTCAG
    TACTATAAGT TGATTGAAGA ATGTATTTCC CAGATAGTTC TGCACAAGAA CGGGGCTGAT
    CCTGACTTCA AGTGCCGGCA CCTCCAGATT GAGATTGAGG GATTAATTGA TCAAATGATT
    GATAAGACAA AGGTGGAGAA ATCTGAAGCC AAAGCTGCAG AGCTGGAAAA GAAGTTGGAC
    TCAGAGTTAA CAGCCCGACA TGAGCTACAG GTGGAAATGA AAAAGATGGA AAGTGACTTT
    GAGCAGAAGC TTCAAGATCT TCAGGGAGAA AAAGATGCAC TGCATTCTGA AAAGCAGCAA
    ATTGCCACAG AGAAACAGGA CCTGGAAGCA GAGGTGTCCC AGCTCACAGG AGAGGTTGCC
    AAGCTGACAA AGGAACTGGA AGATGCCAAG AAAGAAATGG CTTCCCTCTC TGCGGCAGCT
    ATTACTGTAC CTCCTTCTGT TCCTAGTCGT GCTCCTGTTC CCCCTGCCCC TCCTTTACCT
    GGTGACTCTG GCACTATTAT TCCACCACCA CCTGCTCCTG GGGATAGTAC CACTCCTCCT
    CCTCCTCCTC CTCCTCCTCC TCCTCCACCT CCTTTGCCTG GGGGTGTTTG CATCTCCTCA
    CCCCCTTCTT TACCTGGAGG TACTGCTATC TCTCCACCCC CTCCTTTGTC TGGGGATGCT
    ACCATCCCTC CACCCCCTCC TTTGCCTGAG GGTGTTGGCA TCCCTTCACC CTCTTCTTTG
    CCTGGAGGTA CTGCCATCCC CCCACCTCCT CCTTTGCCTG GGAGTGCTAG AATCCCCCCA
    CCACCACCTC CTTTGCCTGG GAGTGCTGGA ATTCCCCCCC CACCTCCTCC CTTGCCTGGA
    GAAGCAGGAA TGCCACCTCC TCCTCCCCCT CTTCCTGGTG GTCCTGGAAT CCCTCCACCT
    CCTCCATTTC CCGGAGGCCC TGGCATTCCT CCACCTCCAC CCGGAATGGG TATGCCTCCA
    CCTCCCCCAT TTGGATTTGG AGTTCCTGCA GCCCCAGTTC TGCCATTTGG ATTAACCCCC
    AAAAAGCTTT ATAAGCCAGA GGTGCAGCTC CGGAGGCCAA ACTGGTCCAA GCTTGTGGCT
    GAGGACCTCT CCCAGGACTG CTTCTGGACA AAGGTGAAGG AGGACCGCTT TGAGAACAAT
    GAACTTTTCG CCAAACTTAC CCTTACCTTC TCTGCCCAGA CCAAGACTTC CAAAGCCAAG
    AAGGATCAAG AAGGTGGAGA AGAAAAGAAA TCTGTGCAAA AGAAAAAAGT AAAAGAGTTA
    AAGGTGTTGG ATTCAAAGAC AGCCCAGAAT CTCTCAATCT TTTTGGGTTC CTTCCGCATG
    CCCTATCAAG AGATTAAGAA TGTCATCCTG GAGGTGAATG AGGCTGTTCT GACTGAGTCT
    ATGATCCAGA ACCTCATTAA GCAAATGCCA GAGCCAGAGC AGTTAAAAAT GCTTTCTGAA
    CTGAAGGATG AATATGATGA CCTGGCTGAG TCAGAGCAGT TTGGCGTGGT GATGGGCACT
    GTGCCCCGAC TGCGGCCTCG CCTCAATGCC ATTCTCTTCA AGCTACAATT CAGCGAGCAA
    GTGGAGAATA TCAAGCCAGA GATTGTGTCT GTCACTGCTG CATGTGAGGA GTTACGTAAG
    AGTGAGAGCT TTTCCAATCT CCTAGAGATT ACCTTGCTTG TTGGAAATTA CATGAATGCT
    GGCTCCAGAA ATGCTGGTGC TTTTGGCTTC AATATCAGCT TCCTCTGTAA GCTTCGAGAC
    ACCAAGTCCA CAGATCAGAA GATGACGTTG TTACACTTCT TGGCTGAGTT GTGTGAGAAT
    GACTATCCCG ATGTCCTCAA GTTTCCAGAC GAGCTTGCCC ATGTGGAGAA AGCCAGCCGA
    GTTTCTGCTG AAAACTTGCA AAAGAACCTA GATCAGATGA AGAAACAAAT TTCTGATGTG
    GAACGTGATG TTCAGAATTT CCCAGCTGCC ACAGATGAAA AAGACAAGTT TGTTGAAAAA
    ATGACCAGCT TTGTGAAGGA TGCACAGGAA CAGTATAACA AGCTGCGGAT GATGCATTCT
    AACATGGAGA CCCTCTATAA GGAGCTGGGC GAGTACTTCC TCTTTGACCC CAAGAAGTTG
    TCTGTTGAAG AATTTTTCAT GGATCTTCAC AATTTTCGGA ATATGTTTTT GCAAGCAGTC
    AAGGAGAACC AGAAGCGGCG GGAGACAGAA GAAAAGATGA GGCGAGCAAA ACTAGCCAAG
    GAGAAGGCAG AGAAGGAGCG GCTAGAGAAG CAGCAGAAGA GAGAGCAACT CATAGACATG
    AATGCAGAGG GCGATGAGAC AGGTGTGATG GACAGTCTTC TAGAAGCCCT GCAGTCAGGG
    GCAGCATTCC GACGGAAGAG AGGGCCCCGT CAAGCCAACA GGAAGGCCGG GTGTGCAGTC
    ACATCTCTGC TAGCTTCGGA GCTGACCAAG GATGATGCCA TGGCTGCTGT TCCTGCCAAG
    GTGTCCAAGA ACAGTGAGAC ATTCCCCACA ATCCTTGAGG AAGCCAAGGA GTTGGTTGGC
    CGTGCAAGCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001073280.1
    CDS87..3878
    Translation

    Target ORF information:

    RefSeq Version NM_001079812.3
    Organism Homo sapiens(human)
    Definition Homo sapiens diaphanous related formin 1 (DIAPH1), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001079812.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGAGCCGC CCGGCGGGAG CCTGGGGCCC GGCCGCGGGA CCCGGGACAA GAAGAAGGGC 
    CGGAGCCCAG ATGAGCTGCC CTCGGCGGGC GGCGACGGCG GCAAATCTAA GAAATTTCTG
    GAGAGATTTA CCAGCATGAG AATTAAGAAG GAGAAGGAAA AGCCCAATTC TGCTCATAGA
    AATTCTTCTG CATCATATGG GGATGATCCC ACAGCACAGT CATTGCAAGA TGTTTCAGAT
    GAACAAGTGC TGGTTCTCTT TGAACAGATG CTGCTGGATA TGAACCTGAA TGAGGAGAAA
    CAGCAACCTT TGAGGGAGAA GGACATCATC ATCAAGAGGG AGATGGTGTC CCAATACTTG
    TACACCTCCA AGGCTGGCAT GAGCCAGAAG GAGAGCTCTA AGTCTGCCAT GATGTATATT
    CAGGAGTTGA GGTCAGGCTT GCGGGATATG CCTCTGCTCA GCTGCCTGGA GTCCCTTCGT
    GTGTCTCTCA ACAACAACCC TGTCAGTTGG GTGCAAACAT TTGGTGCTGA AGGCTTGGCC
    TCCTTATTGG ACATTCTTAA ACGACTTCAT GATGAGAAAG AAGAGACTGC TGGGAGTTAC
    GATAGCCGGA ACAAGCATGA GATCATTCGC TGCTTGAAAG CTTTTATGAA CAACAAGTTT
    GGAATCAAGA CCATGTTGGA GACAGAAGAA GGAATCCTAC TGCTGGTCAG AGCCATGGAT
    CCTGCTGTTC CCAACATGAT GATTGATGCA GCTAAGCTGC TTTCTGCTCT TTGTATTCTA
    CCGCAGCCAG AGGACATGAA TGAAAGGGTT TTGGAGGCAA TGACAGAAAG AGCTGAGATG
    GATGAAGTGG AACGTTTCCA GCCGCTGCTG GATGGATTAA AAAGTGGAAC CACTATTGCA
    CTGAAGGTTG GATGCCTACA GCTGATCAAT GCTCTCATCA CACCAGCGGA GGAACTTGAC
    TTCCGAGTTC ACATCAGAAG TGAACTGATG CGTTTGGGGC TACATCAGGT GTTGCAGGAC
    CTTCGAGAGA TTGAAAATGA AGATATGAGA GTGCAACTAA ATGTGTTTGA TGAACAAGGG
    GAAGAGGATT CCTATGACCT GAAGGGACGG CTGGATGACA TTCGCATGGA GATGGATGAC
    TTTAATGAAG TCTTTCAGAT TCTCTTAAAC ACAGTGAAGG ATTCAAAGGC AGAGCCACAC
    TTCCTTTCCA TCCTGCAGCA CTTACTCTTG GTCCGAAATG ACTATGAGGC CAGACCTCAG
    TACTATAAGT TGATTGAAGA ATGTATTTCC CAGATAGTTC TGCACAAGAA CGGGGCTGAT
    CCTGACTTCA AGTGCCGGCA CCTCCAGATT GAGATTGAGG GATTAATTGA TCAAATGATT
    GATAAGACAA AGGTGGAGAA ATCTGAAGCC AAAGCTGCAG AGCTGGAAAA GAAGTTGGAC
    TCAGAGTTAA CAGCCCGACA TGAGCTACAG GTGGAAATGA AAAAGATGGA AAGTGACTTT
    GAGCAGAAGC TTCAAGATCT TCAGGGAGAA AAAGATGCAC TGCATTCTGA AAAGCAGCAA
    ATTGCCACAG AGAAACAGGA CCTGGAAGCA GAGGTGTCCC AGCTCACAGG AGAGGTTGCC
    AAGCTGACAA AGGAACTGGA AGATGCCAAG AAAGAAATGG CTTCCCTCTC TGCGGCAGCT
    ATTACTGTAC CTCCTTCTGT TCCTAGTCGT GCTCCTGTTC CCCCTGCCCC TCCTTTACCT
    GGTGACTCTG GCACTATTAT TCCACCACCA CCTGCTCCTG GGGATAGTAC CACTCCTCCT
    CCTCCTCCTC CTCCTCCTCC TCCTCCACCT CCTTTGCCTG GGGGTGTTTG CATCTCCTCA
    CCCCCTTCTT TACCTGGAGG TACTGCTATC TCTCCACCCC CTCCTTTGTC TGGGGATGCT
    ACCATCCCTC CACCCCCTCC TTTGCCTGAG GGTGTTGGCA TCCCTTCACC CTCTTCTTTG
    CCTGGAGGTA CTGCCATCCC CCCACCTCCT CCTTTGCCTG GGAGTGCTAG AATCCCCCCA
    CCACCACCTC CTTTGCCTGG GAGTGCTGGA ATTCCCCCCC CACCTCCTCC CTTGCCTGGA
    GAAGCAGGAA TGCCACCTCC TCCTCCCCCT CTTCCTGGTG GTCCTGGAAT CCCTCCACCT
    CCTCCATTTC CCGGAGGCCC TGGCATTCCT CCACCTCCAC CCGGAATGGG TATGCCTCCA
    CCTCCCCCAT TTGGATTTGG AGTTCCTGCA GCCCCAGTTC TGCCATTTGG ATTAACCCCC
    AAAAAGCTTT ATAAGCCAGA GGTGCAGCTC CGGAGGCCAA ACTGGTCCAA GCTTGTGGCT
    GAGGACCTCT CCCAGGACTG CTTCTGGACA AAGGTGAAGG AGGACCGCTT TGAGAACAAT
    GAACTTTTCG CCAAACTTAC CCTTACCTTC TCTGCCCAGA CCAAGACTTC CAAAGCCAAG
    AAGGATCAAG AAGGTGGAGA AGAAAAGAAA TCTGTGCAAA AGAAAAAAGT AAAAGAGTTA
    AAGGTGTTGG ATTCAAAGAC AGCCCAGAAT CTCTCAATCT TTTTGGGTTC CTTCCGCATG
    CCCTATCAAG AGATTAAGAA TGTCATCCTG GAGGTGAATG AGGCTGTTCT GACTGAGTCT
    ATGATCCAGA ACCTCATTAA GCAAATGCCA GAGCCAGAGC AGTTAAAAAT GCTTTCTGAA
    CTGAAGGATG AATATGATGA CCTGGCTGAG TCAGAGCAGT TTGGCGTGGT GATGGGCACT
    GTGCCCCGAC TGCGGCCTCG CCTCAATGCC ATTCTCTTCA AGCTACAATT CAGCGAGCAA
    GTGGAGAATA TCAAGCCAGA GATTGTGTCT GTCACTGCTG CATGTGAGGA GTTACGTAAG
    AGTGAGAGCT TTTCCAATCT CCTAGAGATT ACCTTGCTTG TTGGAAATTA CATGAATGCT
    GGCTCCAGAA ATGCTGGTGC TTTTGGCTTC AATATCAGCT TCCTCTGTAA GCTTCGAGAC
    ACCAAGTCCA CAGATCAGAA GATGACGTTG TTACACTTCT TGGCTGAGTT GTGTGAGAAT
    GACTATCCCG ATGTCCTCAA GTTTCCAGAC GAGCTTGCCC ATGTGGAGAA AGCCAGCCGA
    GTTTCTGCTG AAAACTTGCA AAAGAACCTA GATCAGATGA AGAAACAAAT TTCTGATGTG
    GAACGTGATG TTCAGAATTT CCCAGCTGCC ACAGATGAAA AAGACAAGTT TGTTGAAAAA
    ATGACCAGCT TTGTGAAGGA TGCACAGGAA CAGTATAACA AGCTGCGGAT GATGCATTCT
    AACATGGAGA CCCTCTATAA GGAGCTGGGC GAGTACTTCC TCTTTGACCC CAAGAAGTTG
    TCTGTTGAAG AATTTTTCAT GGATCTTCAC AATTTTCGGA ATATGTTTTT GCAAGCAGTC
    AAGGAGAACC AGAAGCGGCG GGAGACAGAA GAAAAGATGA GGCGAGCAAA ACTAGCCAAG
    GAGAAGGCAG AGAAGGAGCG GCTAGAGAAG CAGCAGAAGA GAGAGCAACT CATAGACATG
    AATGCAGAGG GCGATGAGAC AGGTGTGATG GACAGTCTTC TAGAAGCCCT GCAGTCAGGG
    GCAGCATTCC GACGGAAGAG AGGGCCCCGT CAAGCCAACA GGAAGGCCGG GTGTGCAGTC
    ACATCTCTGC TAGCTTCGGA GCTGACCAAG GATGATGCCA TGGCTGCTGT TCCTGCCAAG
    GTGTCCAAGA ACAGTGAGAC ATTCCCCACA ATCCTTGAGG AAGCCAAGGA GTTGGTTGGC
    CGTGCAAGCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu39824
    Clone ID Related Accession (Same CDS sequence) NM_001314007.1 , NM_001314007.2
    Accession Version NM_001314007.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3753bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-25
    Organism Homo sapiens(human)
    Product protein diaphanous homolog 1 isoform 3
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA593734.1, BC143414.1, AC008781.7 and AY007129.1. On Sep 23, 2015 this sequence version replaced XM_005268384.2. Transcript Variant: This variant (3) contains an alternate exon in the 3' end compared to variant 1, that causes a frameshift. The resulting isoform (3) has a shorter and distinct C-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC143414.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGAGCCGC CCGGCGGGAG CCTGGGGCCC GGCCGCGGGA CCCGGGACAA GAAGAAGGGC 
    CGGAGCCCAG ATGAGCTGCC CTCGGCGGGC GGCGACGGCG GCAAATCTAA GAAATTTACT
    CTGAAGCGGC TCATGGCAGA TGAGCTGGAG AGATTTACCA GCATGAGAAT TAAGAAGGAG
    AAGGAAAAGC CCAATTCTGC TCATAGAAAT TCTTCTGCAT CATATGGGGA TGATCCCACA
    GCACAGTCAT TGCAAGATGT TTCAGATGAA CAAGTGCTGG TTCTCTTTGA ACAGATGCTG
    CTGGATATGA ACCTGAATGA GGAGAAACAG CAACCTTTGA GGGAGAAGGA CATCATCATC
    AAGAGGGAGA TGGTGTCCCA ATACTTGTAC ACCTCCAAGG CTGGCATGAG CCAGAAGGAG
    AGCTCTAAGT CTGCCATGAT GTATATTCAG GAGTTGAGGT CAGGCTTGCG GGATATGCCT
    CTGCTCAGCT GCCTGGAGTC CCTTCGTGTG TCTCTCAACA ACAACCCTGT CAGTTGGGTG
    CAAACATTTG GTGCTGAAGG CTTGGCCTCC TTATTGGACA TTCTTAAACG ACTTCATGAT
    GAGAAAGAAG AGACTGCTGG GAGTTACGAT AGCCGGAACA AGCATGAGAT CATTCGCTGC
    TTGAAAGCTT TTATGAACAA CAAGTTTGGA ATCAAGACCA TGTTGGAGAC AGAAGAAGGA
    ATCCTACTGC TGGTCAGAGC CATGGATCCT GCTGTTCCCA ACATGATGAT TGATGCAGCT
    AAGCTGCTTT CTGCTCTTTG TATTCTACCG CAGCCAGAGG ACATGAATGA AAGGGTTTTG
    GAGGCAATGA CAGAAAGAGC TGAGATGGAT GAAGTGGAAC GTTTCCAGCC GCTGCTGGAT
    GGATTAAAAA GTGGAACCAC TATTGCACTG AAGGTTGGAT GCCTACAGCT GATCAATGCT
    CTCATCACAC CAGCGGAGGA ACTTGACTTC CGAGTTCACA TCAGAAGTGA ACTGATGCGT
    TTGGGGCTAC ATCAGGTGTT GCAGGACCTT CGAGAGATTG AAAATGAAGA TATGAGAGTG
    CAACTAAATG TGTTTGATGA ACAAGGGGAA GAGGATTCCT ATGACCTGAA GGGACGGCTG
    GATGACATTC GCATGGAGAT GGATGACTTT AATGAAGTCT TTCAGATTCT CTTAAACACA
    GTGAAGGATT CAAAGGCAGA GCCACACTTC CTTTCCATCC TGCAGCACTT ACTCTTGGTC
    CGAAATGACT ATGAGGCCAG ACCTCAGTAC TATAAGTTGA TTGAAGAATG TATTTCCCAG
    ATAGTTCTGC ACAAGAACGG GGCTGATCCT GACTTCAAGT GCCGGCACCT CCAGATTGAG
    ATTGAGGGAT TAATTGATCA AATGATTGAT AAGACAAAGG TGGAGAAATC TGAAGCCAAA
    GCTGCAGAGC TGGAAAAGAA GTTGGACTCA GAGTTAACAG CCCGACATGA GCTACAGGTG
    GAAATGAAAA AGATGGAAAG TGACTTTGAG CAGAAGCTTC AAGATCTTCA GGGAGAAAAA
    GATGCACTGC ATTCTGAAAA GCAGCAAATT GCCACAGAGA AACAGGACCT GGAAGCAGAG
    GTGTCCCAGC TCACAGGAGA GGTTGCCAAG CTGACAAAGG AACTGGAAGA TGCCAAGAAA
    GAAATGGCTT CCCTCTCTGC GGCAGCTATT ACTGTACCTC CTTCTGTTCC TAGTCGTGCT
    CCTGTTCCCC CTGCCCCTCC TTTACCTGGT GACTCTGGCA CTATTATTCC ACCACCACCT
    GCTCCTGGGG ATAGTACCAC TCCTCCTCCT CCTCCTCCTC CTCCTCCTCC TCCACCTCCT
    TTGCCTGGGG GTGTTTGCAT CTCCTCACCC CCTTCTTTAC CTGGAGGTAC TGCTATCTCT
    CCACCCCCTC CTTTGTCTGG GGATGCTACC ATCCCTCCAC CCCCTCCTTT GCCTGAGGGT
    GTTGGCATCC CTTCACCCTC TTCTTTGCCT GGAGGTACTG CCATCCCCCC ACCTCCTCCT
    TTGCCTGGGA GTGCTAGAAT CCCCCCACCA CCACCTCCTT TGCCTGGGAG TGCTGGAATT
    CCCCCCCCAC CTCCTCCCTT GCCTGGAGAA GCAGGAATGC CACCTCCTCC TCCCCCTCTT
    CCTGGTGGTC CTGGAATCCC TCCACCTCCT CCATTTCCCG GAGGCCCTGG CATTCCTCCA
    CCTCCACCCG GAATGGGTAT GCCTCCACCT CCCCCATTTG GATTTGGAGT TCCTGCAGCC
    CCAGTTCTGC CATTTGGATT AACCCCCAAA AAGCTTTATA AGCCAGAGGT GCAGCTCCGG
    AGGCCAAACT GGTCCAAGCT TGTGGCTGAG GACCTCTCCC AGGACTGCTT CTGGACAAAG
    GTGAAGGAGG ACCGCTTTGA GAACAATGAA CTTTTCGCCA AACTTACCCT TACCTTCTCT
    GCCCAGACCA AGACTTCCAA AGCCAAGAAG GATCAAGAAG GTGGAGAAGA AAAGAAATCT
    GTGCAAAAGA AAAAAGTAAA AGAGTTAAAG GTGTTGGATT CAAAGACAGC CCAGAATCTC
    TCAATCTTTT TGGGTTCCTT CCGCATGCCC TATCAAGAGA TTAAGAATGT CATCCTGGAG
    GTGAATGAGG CTGTTCTGAC TGAGTCTATG ATCCAGAACC TCATTAAGCA AATGCCAGAG
    CCAGAGCAGT TAAAAATGCT TTCTGAACTG AAGGATGAAT ATGATGACCT GGCTGAGTCA
    GAGCAGTTTG GCGTGGTGAT GGGCACTGTG CCCCGACTGC GGCCTCGCCT CAATGCCATT
    CTCTTCAAGC TACAATTCAG CGAGCAAGTG GAGAATATCA AGCCAGAGAT TGTGTCTGTC
    ACTGCTGCAT GTGAGGAGTT ACGTAAGAGT GAGAGCTTTT CCAATCTCCT AGAGATTACC
    TTGCTTGTTG GAAATTACAT GAATGCTGGC TCCAGAAATG CTGGTGCTTT TGGCTTCAAT
    ATCAGCTTCC TCTGTAAGCT TCGAGACACC AAGTCCACAG ATCAGAAGAT GACGTTGTTA
    CACTTCTTGG CTGAGTTGTG TGAGAATGAC TATCCCGATG TCCTCAAGTT TCCAGACGAG
    CTTGCCCATG TGGAGAAAGC CAGCCGAGTT TCTGCTGAAA ACTTGCAAAA GAACCTAGAT
    CAGATGAAGA AACAAATTTC TGATGTGGAA CGTGATGTTC AGAATTTCCC AGCTGCCACA
    GATGAAAAAG ACAAGTTTGT TGAAAAAATG ACCAGCTTTG TGAAGGATGC ACAGGAACAG
    TATAACAAGC TGCGGATGAT GCATTCTAAC ATGGAGACCC TCTATAAGGA GCTGGGCGAG
    TACTTCCTCT TTGACCCCAA GAAGTTGTCT GTTGAAGAAT TTTTCATGGA TCTTCACAAT
    TTTCGGAATA TGTTTTTGCA AGCAGTCAAG GAGAACCAGA AGCGGCGGGA GACAGAAGAA
    AAGATGAGGC GAGCAAAACT AGCCAAGGAG AAGGCAGAGA AGGAGCGGCT AGAGAAGCAG
    CAGAAGAGAG AGCAACTCAT AGACATGAAT GCAGAGGGCG ATGAGACAGG TGTGATGGAC
    AGTCTTCTAG AAGCCCTGCA GTCAGGGGCA GCATTCCGAC GGAAGAGAGG GCCCCGTCAA
    GACAACCTCT TGTGCCCCTG GGAAGTAGAG GAGGAGGAGG AGACCTTTAA ATTGCCAACA
    GGAAGGCCGG GTGTGCAGTC ACATCTCTGC TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001300936.1
    CDS142..3894
    Translation

    Target ORF information:

    RefSeq Version NM_001314007.1
    Organism Homo sapiens(human)
    Definition Homo sapiens diaphanous related formin 1 (DIAPH1), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001314007.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGAGCCGC CCGGCGGGAG CCTGGGGCCC GGCCGCGGGA CCCGGGACAA GAAGAAGGGC 
    CGGAGCCCAG ATGAGCTGCC CTCGGCGGGC GGCGACGGCG GCAAATCTAA GAAATTTACT
    CTGAAGCGGC TCATGGCAGA TGAGCTGGAG AGATTTACCA GCATGAGAAT TAAGAAGGAG
    AAGGAAAAGC CCAATTCTGC TCATAGAAAT TCTTCTGCAT CATATGGGGA TGATCCCACA
    GCACAGTCAT TGCAAGATGT TTCAGATGAA CAAGTGCTGG TTCTCTTTGA ACAGATGCTG
    CTGGATATGA ACCTGAATGA GGAGAAACAG CAACCTTTGA GGGAGAAGGA CATCATCATC
    AAGAGGGAGA TGGTGTCCCA ATACTTGTAC ACCTCCAAGG CTGGCATGAG CCAGAAGGAG
    AGCTCTAAGT CTGCCATGAT GTATATTCAG GAGTTGAGGT CAGGCTTGCG GGATATGCCT
    CTGCTCAGCT GCCTGGAGTC CCTTCGTGTG TCTCTCAACA ACAACCCTGT CAGTTGGGTG
    CAAACATTTG GTGCTGAAGG CTTGGCCTCC TTATTGGACA TTCTTAAACG ACTTCATGAT
    GAGAAAGAAG AGACTGCTGG GAGTTACGAT AGCCGGAACA AGCATGAGAT CATTCGCTGC
    TTGAAAGCTT TTATGAACAA CAAGTTTGGA ATCAAGACCA TGTTGGAGAC AGAAGAAGGA
    ATCCTACTGC TGGTCAGAGC CATGGATCCT GCTGTTCCCA ACATGATGAT TGATGCAGCT
    AAGCTGCTTT CTGCTCTTTG TATTCTACCG CAGCCAGAGG ACATGAATGA AAGGGTTTTG
    GAGGCAATGA CAGAAAGAGC TGAGATGGAT GAAGTGGAAC GTTTCCAGCC GCTGCTGGAT
    GGATTAAAAA GTGGAACCAC TATTGCACTG AAGGTTGGAT GCCTACAGCT GATCAATGCT
    CTCATCACAC CAGCGGAGGA ACTTGACTTC CGAGTTCACA TCAGAAGTGA ACTGATGCGT
    TTGGGGCTAC ATCAGGTGTT GCAGGACCTT CGAGAGATTG AAAATGAAGA TATGAGAGTG
    CAACTAAATG TGTTTGATGA ACAAGGGGAA GAGGATTCCT ATGACCTGAA GGGACGGCTG
    GATGACATTC GCATGGAGAT GGATGACTTT AATGAAGTCT TTCAGATTCT CTTAAACACA
    GTGAAGGATT CAAAGGCAGA GCCACACTTC CTTTCCATCC TGCAGCACTT ACTCTTGGTC
    CGAAATGACT ATGAGGCCAG ACCTCAGTAC TATAAGTTGA TTGAAGAATG TATTTCCCAG
    ATAGTTCTGC ACAAGAACGG GGCTGATCCT GACTTCAAGT GCCGGCACCT CCAGATTGAG
    ATTGAGGGAT TAATTGATCA AATGATTGAT AAGACAAAGG TGGAGAAATC TGAAGCCAAA
    GCTGCAGAGC TGGAAAAGAA GTTGGACTCA GAGTTAACAG CCCGACATGA GCTACAGGTG
    GAAATGAAAA AGATGGAAAG TGACTTTGAG CAGAAGCTTC AAGATCTTCA GGGAGAAAAA
    GATGCACTGC ATTCTGAAAA GCAGCAAATT GCCACAGAGA AACAGGACCT GGAAGCAGAG
    GTGTCCCAGC TCACAGGAGA GGTTGCCAAG CTGACAAAGG AACTGGAAGA TGCCAAGAAA
    GAAATGGCTT CCCTCTCTGC GGCAGCTATT ACTGTACCTC CTTCTGTTCC TAGTCGTGCT
    CCTGTTCCCC CTGCCCCTCC TTTACCTGGT GACTCTGGCA CTATTATTCC ACCACCACCT
    GCTCCTGGGG ATAGTACCAC TCCTCCTCCT CCTCCTCCTC CTCCTCCTCC TCCACCTCCT
    TTGCCTGGGG GTGTTTGCAT CTCCTCACCC CCTTCTTTAC CTGGAGGTAC TGCTATCTCT
    CCACCCCCTC CTTTGTCTGG GGATGCTACC ATCCCTCCAC CCCCTCCTTT GCCTGAGGGT
    GTTGGCATCC CTTCACCCTC TTCTTTGCCT GGAGGTACTG CCATCCCCCC ACCTCCTCCT
    TTGCCTGGGA GTGCTAGAAT CCCCCCACCA CCACCTCCTT TGCCTGGGAG TGCTGGAATT
    CCCCCCCCAC CTCCTCCCTT GCCTGGAGAA GCAGGAATGC CACCTCCTCC TCCCCCTCTT
    CCTGGTGGTC CTGGAATCCC TCCACCTCCT CCATTTCCCG GAGGCCCTGG CATTCCTCCA
    CCTCCACCCG GAATGGGTAT GCCTCCACCT CCCCCATTTG GATTTGGAGT TCCTGCAGCC
    CCAGTTCTGC CATTTGGATT AACCCCCAAA AAGCTTTATA AGCCAGAGGT GCAGCTCCGG
    AGGCCAAACT GGTCCAAGCT TGTGGCTGAG GACCTCTCCC AGGACTGCTT CTGGACAAAG
    GTGAAGGAGG ACCGCTTTGA GAACAATGAA CTTTTCGCCA AACTTACCCT TACCTTCTCT
    GCCCAGACCA AGACTTCCAA AGCCAAGAAG GATCAAGAAG GTGGAGAAGA AAAGAAATCT
    GTGCAAAAGA AAAAAGTAAA AGAGTTAAAG GTGTTGGATT CAAAGACAGC CCAGAATCTC
    TCAATCTTTT TGGGTTCCTT CCGCATGCCC TATCAAGAGA TTAAGAATGT CATCCTGGAG
    GTGAATGAGG CTGTTCTGAC TGAGTCTATG ATCCAGAACC TCATTAAGCA AATGCCAGAG
    CCAGAGCAGT TAAAAATGCT TTCTGAACTG AAGGATGAAT ATGATGACCT GGCTGAGTCA
    GAGCAGTTTG GCGTGGTGAT GGGCACTGTG CCCCGACTGC GGCCTCGCCT CAATGCCATT
    CTCTTCAAGC TACAATTCAG CGAGCAAGTG GAGAATATCA AGCCAGAGAT TGTGTCTGTC
    ACTGCTGCAT GTGAGGAGTT ACGTAAGAGT GAGAGCTTTT CCAATCTCCT AGAGATTACC
    TTGCTTGTTG GAAATTACAT GAATGCTGGC TCCAGAAATG CTGGTGCTTT TGGCTTCAAT
    ATCAGCTTCC TCTGTAAGCT TCGAGACACC AAGTCCACAG ATCAGAAGAT GACGTTGTTA
    CACTTCTTGG CTGAGTTGTG TGAGAATGAC TATCCCGATG TCCTCAAGTT TCCAGACGAG
    CTTGCCCATG TGGAGAAAGC CAGCCGAGTT TCTGCTGAAA ACTTGCAAAA GAACCTAGAT
    CAGATGAAGA AACAAATTTC TGATGTGGAA CGTGATGTTC AGAATTTCCC AGCTGCCACA
    GATGAAAAAG ACAAGTTTGT TGAAAAAATG ACCAGCTTTG TGAAGGATGC ACAGGAACAG
    TATAACAAGC TGCGGATGAT GCATTCTAAC ATGGAGACCC TCTATAAGGA GCTGGGCGAG
    TACTTCCTCT TTGACCCCAA GAAGTTGTCT GTTGAAGAAT TTTTCATGGA TCTTCACAAT
    TTTCGGAATA TGTTTTTGCA AGCAGTCAAG GAGAACCAGA AGCGGCGGGA GACAGAAGAA
    AAGATGAGGC GAGCAAAACT AGCCAAGGAG AAGGCAGAGA AGGAGCGGCT AGAGAAGCAG
    CAGAAGAGAG AGCAACTCAT AGACATGAAT GCAGAGGGCG ATGAGACAGG TGTGATGGAC
    AGTCTTCTAG AAGCCCTGCA GTCAGGGGCA GCATTCCGAC GGAAGAGAGG GCCCCGTCAA
    GACAACCTCT TGTGCCCCTG GGAAGTAGAG GAGGAGGAGG AGACCTTTAA ATTGCCAACA
    GGAAGGCCGG GTGTGCAGTC ACATCTCTGC TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu39824
    Clone ID Related Accession (Same CDS sequence) NM_001314007.1 , NM_001314007.2
    Accession Version NM_001314007.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3753bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-26
    Organism Homo sapiens(human)
    Product protein diaphanous homolog 1 isoform 3
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA593734.1, BC143414.1, AC008781.7 and AY007129.1. On May 31, 2019 this sequence version replaced NM_001314007.1. Transcript Variant: This variant (3) contains an alternate exon in the 3' end compared to variant 1, that causes a frameshift. The resulting isoform (3) has a shorter and distinct C-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC143414.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGAGCCGC CCGGCGGGAG CCTGGGGCCC GGCCGCGGGA CCCGGGACAA GAAGAAGGGC 
    CGGAGCCCAG ATGAGCTGCC CTCGGCGGGC GGCGACGGCG GCAAATCTAA GAAATTTACT
    CTGAAGCGGC TCATGGCAGA TGAGCTGGAG AGATTTACCA GCATGAGAAT TAAGAAGGAG
    AAGGAAAAGC CCAATTCTGC TCATAGAAAT TCTTCTGCAT CATATGGGGA TGATCCCACA
    GCACAGTCAT TGCAAGATGT TTCAGATGAA CAAGTGCTGG TTCTCTTTGA ACAGATGCTG
    CTGGATATGA ACCTGAATGA GGAGAAACAG CAACCTTTGA GGGAGAAGGA CATCATCATC
    AAGAGGGAGA TGGTGTCCCA ATACTTGTAC ACCTCCAAGG CTGGCATGAG CCAGAAGGAG
    AGCTCTAAGT CTGCCATGAT GTATATTCAG GAGTTGAGGT CAGGCTTGCG GGATATGCCT
    CTGCTCAGCT GCCTGGAGTC CCTTCGTGTG TCTCTCAACA ACAACCCTGT CAGTTGGGTG
    CAAACATTTG GTGCTGAAGG CTTGGCCTCC TTATTGGACA TTCTTAAACG ACTTCATGAT
    GAGAAAGAAG AGACTGCTGG GAGTTACGAT AGCCGGAACA AGCATGAGAT CATTCGCTGC
    TTGAAAGCTT TTATGAACAA CAAGTTTGGA ATCAAGACCA TGTTGGAGAC AGAAGAAGGA
    ATCCTACTGC TGGTCAGAGC CATGGATCCT GCTGTTCCCA ACATGATGAT TGATGCAGCT
    AAGCTGCTTT CTGCTCTTTG TATTCTACCG CAGCCAGAGG ACATGAATGA AAGGGTTTTG
    GAGGCAATGA CAGAAAGAGC TGAGATGGAT GAAGTGGAAC GTTTCCAGCC GCTGCTGGAT
    GGATTAAAAA GTGGAACCAC TATTGCACTG AAGGTTGGAT GCCTACAGCT GATCAATGCT
    CTCATCACAC CAGCGGAGGA ACTTGACTTC CGAGTTCACA TCAGAAGTGA ACTGATGCGT
    TTGGGGCTAC ATCAGGTGTT GCAGGACCTT CGAGAGATTG AAAATGAAGA TATGAGAGTG
    CAACTAAATG TGTTTGATGA ACAAGGGGAA GAGGATTCCT ATGACCTGAA GGGACGGCTG
    GATGACATTC GCATGGAGAT GGATGACTTT AATGAAGTCT TTCAGATTCT CTTAAACACA
    GTGAAGGATT CAAAGGCAGA GCCACACTTC CTTTCCATCC TGCAGCACTT ACTCTTGGTC
    CGAAATGACT ATGAGGCCAG ACCTCAGTAC TATAAGTTGA TTGAAGAATG TATTTCCCAG
    ATAGTTCTGC ACAAGAACGG GGCTGATCCT GACTTCAAGT GCCGGCACCT CCAGATTGAG
    ATTGAGGGAT TAATTGATCA AATGATTGAT AAGACAAAGG TGGAGAAATC TGAAGCCAAA
    GCTGCAGAGC TGGAAAAGAA GTTGGACTCA GAGTTAACAG CCCGACATGA GCTACAGGTG
    GAAATGAAAA AGATGGAAAG TGACTTTGAG CAGAAGCTTC AAGATCTTCA GGGAGAAAAA
    GATGCACTGC ATTCTGAAAA GCAGCAAATT GCCACAGAGA AACAGGACCT GGAAGCAGAG
    GTGTCCCAGC TCACAGGAGA GGTTGCCAAG CTGACAAAGG AACTGGAAGA TGCCAAGAAA
    GAAATGGCTT CCCTCTCTGC GGCAGCTATT ACTGTACCTC CTTCTGTTCC TAGTCGTGCT
    CCTGTTCCCC CTGCCCCTCC TTTACCTGGT GACTCTGGCA CTATTATTCC ACCACCACCT
    GCTCCTGGGG ATAGTACCAC TCCTCCTCCT CCTCCTCCTC CTCCTCCTCC TCCACCTCCT
    TTGCCTGGGG GTGTTTGCAT CTCCTCACCC CCTTCTTTAC CTGGAGGTAC TGCTATCTCT
    CCACCCCCTC CTTTGTCTGG GGATGCTACC ATCCCTCCAC CCCCTCCTTT GCCTGAGGGT
    GTTGGCATCC CTTCACCCTC TTCTTTGCCT GGAGGTACTG CCATCCCCCC ACCTCCTCCT
    TTGCCTGGGA GTGCTAGAAT CCCCCCACCA CCACCTCCTT TGCCTGGGAG TGCTGGAATT
    CCCCCCCCAC CTCCTCCCTT GCCTGGAGAA GCAGGAATGC CACCTCCTCC TCCCCCTCTT
    CCTGGTGGTC CTGGAATCCC TCCACCTCCT CCATTTCCCG GAGGCCCTGG CATTCCTCCA
    CCTCCACCCG GAATGGGTAT GCCTCCACCT CCCCCATTTG GATTTGGAGT TCCTGCAGCC
    CCAGTTCTGC CATTTGGATT AACCCCCAAA AAGCTTTATA AGCCAGAGGT GCAGCTCCGG
    AGGCCAAACT GGTCCAAGCT TGTGGCTGAG GACCTCTCCC AGGACTGCTT CTGGACAAAG
    GTGAAGGAGG ACCGCTTTGA GAACAATGAA CTTTTCGCCA AACTTACCCT TACCTTCTCT
    GCCCAGACCA AGACTTCCAA AGCCAAGAAG GATCAAGAAG GTGGAGAAGA AAAGAAATCT
    GTGCAAAAGA AAAAAGTAAA AGAGTTAAAG GTGTTGGATT CAAAGACAGC CCAGAATCTC
    TCAATCTTTT TGGGTTCCTT CCGCATGCCC TATCAAGAGA TTAAGAATGT CATCCTGGAG
    GTGAATGAGG CTGTTCTGAC TGAGTCTATG ATCCAGAACC TCATTAAGCA AATGCCAGAG
    CCAGAGCAGT TAAAAATGCT TTCTGAACTG AAGGATGAAT ATGATGACCT GGCTGAGTCA
    GAGCAGTTTG GCGTGGTGAT GGGCACTGTG CCCCGACTGC GGCCTCGCCT CAATGCCATT
    CTCTTCAAGC TACAATTCAG CGAGCAAGTG GAGAATATCA AGCCAGAGAT TGTGTCTGTC
    ACTGCTGCAT GTGAGGAGTT ACGTAAGAGT GAGAGCTTTT CCAATCTCCT AGAGATTACC
    TTGCTTGTTG GAAATTACAT GAATGCTGGC TCCAGAAATG CTGGTGCTTT TGGCTTCAAT
    ATCAGCTTCC TCTGTAAGCT TCGAGACACC AAGTCCACAG ATCAGAAGAT GACGTTGTTA
    CACTTCTTGG CTGAGTTGTG TGAGAATGAC TATCCCGATG TCCTCAAGTT TCCAGACGAG
    CTTGCCCATG TGGAGAAAGC CAGCCGAGTT TCTGCTGAAA ACTTGCAAAA GAACCTAGAT
    CAGATGAAGA AACAAATTTC TGATGTGGAA CGTGATGTTC AGAATTTCCC AGCTGCCACA
    GATGAAAAAG ACAAGTTTGT TGAAAAAATG ACCAGCTTTG TGAAGGATGC ACAGGAACAG
    TATAACAAGC TGCGGATGAT GCATTCTAAC ATGGAGACCC TCTATAAGGA GCTGGGCGAG
    TACTTCCTCT TTGACCCCAA GAAGTTGTCT GTTGAAGAAT TTTTCATGGA TCTTCACAAT
    TTTCGGAATA TGTTTTTGCA AGCAGTCAAG GAGAACCAGA AGCGGCGGGA GACAGAAGAA
    AAGATGAGGC GAGCAAAACT AGCCAAGGAG AAGGCAGAGA AGGAGCGGCT AGAGAAGCAG
    CAGAAGAGAG AGCAACTCAT AGACATGAAT GCAGAGGGCG ATGAGACAGG TGTGATGGAC
    AGTCTTCTAG AAGCCCTGCA GTCAGGGGCA GCATTCCGAC GGAAGAGAGG GCCCCGTCAA
    GACAACCTCT TGTGCCCCTG GGAAGTAGAG GAGGAGGAGG AGACCTTTAA ATTGCCAACA
    GGAAGGCCGG GTGTGCAGTC ACATCTCTGC TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001300936.1
    CDS87..3839
    Translation

    Target ORF information:

    RefSeq Version NM_001314007.2
    Organism Homo sapiens(human)
    Definition Homo sapiens diaphanous related formin 1 (DIAPH1), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001314007.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGAGCCGC CCGGCGGGAG CCTGGGGCCC GGCCGCGGGA CCCGGGACAA GAAGAAGGGC 
    CGGAGCCCAG ATGAGCTGCC CTCGGCGGGC GGCGACGGCG GCAAATCTAA GAAATTTACT
    CTGAAGCGGC TCATGGCAGA TGAGCTGGAG AGATTTACCA GCATGAGAAT TAAGAAGGAG
    AAGGAAAAGC CCAATTCTGC TCATAGAAAT TCTTCTGCAT CATATGGGGA TGATCCCACA
    GCACAGTCAT TGCAAGATGT TTCAGATGAA CAAGTGCTGG TTCTCTTTGA ACAGATGCTG
    CTGGATATGA ACCTGAATGA GGAGAAACAG CAACCTTTGA GGGAGAAGGA CATCATCATC
    AAGAGGGAGA TGGTGTCCCA ATACTTGTAC ACCTCCAAGG CTGGCATGAG CCAGAAGGAG
    AGCTCTAAGT CTGCCATGAT GTATATTCAG GAGTTGAGGT CAGGCTTGCG GGATATGCCT
    CTGCTCAGCT GCCTGGAGTC CCTTCGTGTG TCTCTCAACA ACAACCCTGT CAGTTGGGTG
    CAAACATTTG GTGCTGAAGG CTTGGCCTCC TTATTGGACA TTCTTAAACG ACTTCATGAT
    GAGAAAGAAG AGACTGCTGG GAGTTACGAT AGCCGGAACA AGCATGAGAT CATTCGCTGC
    TTGAAAGCTT TTATGAACAA CAAGTTTGGA ATCAAGACCA TGTTGGAGAC AGAAGAAGGA
    ATCCTACTGC TGGTCAGAGC CATGGATCCT GCTGTTCCCA ACATGATGAT TGATGCAGCT
    AAGCTGCTTT CTGCTCTTTG TATTCTACCG CAGCCAGAGG ACATGAATGA AAGGGTTTTG
    GAGGCAATGA CAGAAAGAGC TGAGATGGAT GAAGTGGAAC GTTTCCAGCC GCTGCTGGAT
    GGATTAAAAA GTGGAACCAC TATTGCACTG AAGGTTGGAT GCCTACAGCT GATCAATGCT
    CTCATCACAC CAGCGGAGGA ACTTGACTTC CGAGTTCACA TCAGAAGTGA ACTGATGCGT
    TTGGGGCTAC ATCAGGTGTT GCAGGACCTT CGAGAGATTG AAAATGAAGA TATGAGAGTG
    CAACTAAATG TGTTTGATGA ACAAGGGGAA GAGGATTCCT ATGACCTGAA GGGACGGCTG
    GATGACATTC GCATGGAGAT GGATGACTTT AATGAAGTCT TTCAGATTCT CTTAAACACA
    GTGAAGGATT CAAAGGCAGA GCCACACTTC CTTTCCATCC TGCAGCACTT ACTCTTGGTC
    CGAAATGACT ATGAGGCCAG ACCTCAGTAC TATAAGTTGA TTGAAGAATG TATTTCCCAG
    ATAGTTCTGC ACAAGAACGG GGCTGATCCT GACTTCAAGT GCCGGCACCT CCAGATTGAG
    ATTGAGGGAT TAATTGATCA AATGATTGAT AAGACAAAGG TGGAGAAATC TGAAGCCAAA
    GCTGCAGAGC TGGAAAAGAA GTTGGACTCA GAGTTAACAG CCCGACATGA GCTACAGGTG
    GAAATGAAAA AGATGGAAAG TGACTTTGAG CAGAAGCTTC AAGATCTTCA GGGAGAAAAA
    GATGCACTGC ATTCTGAAAA GCAGCAAATT GCCACAGAGA AACAGGACCT GGAAGCAGAG
    GTGTCCCAGC TCACAGGAGA GGTTGCCAAG CTGACAAAGG AACTGGAAGA TGCCAAGAAA
    GAAATGGCTT CCCTCTCTGC GGCAGCTATT ACTGTACCTC CTTCTGTTCC TAGTCGTGCT
    CCTGTTCCCC CTGCCCCTCC TTTACCTGGT GACTCTGGCA CTATTATTCC ACCACCACCT
    GCTCCTGGGG ATAGTACCAC TCCTCCTCCT CCTCCTCCTC CTCCTCCTCC TCCACCTCCT
    TTGCCTGGGG GTGTTTGCAT CTCCTCACCC CCTTCTTTAC CTGGAGGTAC TGCTATCTCT
    CCACCCCCTC CTTTGTCTGG GGATGCTACC ATCCCTCCAC CCCCTCCTTT GCCTGAGGGT
    GTTGGCATCC CTTCACCCTC TTCTTTGCCT GGAGGTACTG CCATCCCCCC ACCTCCTCCT
    TTGCCTGGGA GTGCTAGAAT CCCCCCACCA CCACCTCCTT TGCCTGGGAG TGCTGGAATT
    CCCCCCCCAC CTCCTCCCTT GCCTGGAGAA GCAGGAATGC CACCTCCTCC TCCCCCTCTT
    CCTGGTGGTC CTGGAATCCC TCCACCTCCT CCATTTCCCG GAGGCCCTGG CATTCCTCCA
    CCTCCACCCG GAATGGGTAT GCCTCCACCT CCCCCATTTG GATTTGGAGT TCCTGCAGCC
    CCAGTTCTGC CATTTGGATT AACCCCCAAA AAGCTTTATA AGCCAGAGGT GCAGCTCCGG
    AGGCCAAACT GGTCCAAGCT TGTGGCTGAG GACCTCTCCC AGGACTGCTT CTGGACAAAG
    GTGAAGGAGG ACCGCTTTGA GAACAATGAA CTTTTCGCCA AACTTACCCT TACCTTCTCT
    GCCCAGACCA AGACTTCCAA AGCCAAGAAG GATCAAGAAG GTGGAGAAGA AAAGAAATCT
    GTGCAAAAGA AAAAAGTAAA AGAGTTAAAG GTGTTGGATT CAAAGACAGC CCAGAATCTC
    TCAATCTTTT TGGGTTCCTT CCGCATGCCC TATCAAGAGA TTAAGAATGT CATCCTGGAG
    GTGAATGAGG CTGTTCTGAC TGAGTCTATG ATCCAGAACC TCATTAAGCA AATGCCAGAG
    CCAGAGCAGT TAAAAATGCT TTCTGAACTG AAGGATGAAT ATGATGACCT GGCTGAGTCA
    GAGCAGTTTG GCGTGGTGAT GGGCACTGTG CCCCGACTGC GGCCTCGCCT CAATGCCATT
    CTCTTCAAGC TACAATTCAG CGAGCAAGTG GAGAATATCA AGCCAGAGAT TGTGTCTGTC
    ACTGCTGCAT GTGAGGAGTT ACGTAAGAGT GAGAGCTTTT CCAATCTCCT AGAGATTACC
    TTGCTTGTTG GAAATTACAT GAATGCTGGC TCCAGAAATG CTGGTGCTTT TGGCTTCAAT
    ATCAGCTTCC TCTGTAAGCT TCGAGACACC AAGTCCACAG ATCAGAAGAT GACGTTGTTA
    CACTTCTTGG CTGAGTTGTG TGAGAATGAC TATCCCGATG TCCTCAAGTT TCCAGACGAG
    CTTGCCCATG TGGAGAAAGC CAGCCGAGTT TCTGCTGAAA ACTTGCAAAA GAACCTAGAT
    CAGATGAAGA AACAAATTTC TGATGTGGAA CGTGATGTTC AGAATTTCCC AGCTGCCACA
    GATGAAAAAG ACAAGTTTGT TGAAAAAATG ACCAGCTTTG TGAAGGATGC ACAGGAACAG
    TATAACAAGC TGCGGATGAT GCATTCTAAC ATGGAGACCC TCTATAAGGA GCTGGGCGAG
    TACTTCCTCT TTGACCCCAA GAAGTTGTCT GTTGAAGAAT TTTTCATGGA TCTTCACAAT
    TTTCGGAATA TGTTTTTGCA AGCAGTCAAG GAGAACCAGA AGCGGCGGGA GACAGAAGAA
    AAGATGAGGC GAGCAAAACT AGCCAAGGAG AAGGCAGAGA AGGAGCGGCT AGAGAAGCAG
    CAGAAGAGAG AGCAACTCAT AGACATGAAT GCAGAGGGCG ATGAGACAGG TGTGATGGAC
    AGTCTTCTAG AAGCCCTGCA GTCAGGGGCA GCATTCCGAC GGAAGAGAGG GCCCCGTCAA
    GACAACCTCT TGTGCCCCTG GGAAGTAGAG GAGGAGGAGG AGACCTTTAA ATTGCCAACA
    GGAAGGCCGG GTGTGCAGTC ACATCTCTGC TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu57238
    Clone ID Related Accession (Same CDS sequence) XM_011537572.2
    Accession Version XM_011537572.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3783bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product protein diaphanous homolog 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011537572.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGAGAGTAC AGAGTCAGTA TGGTAAGTTA GATAGGAGGA AGACTGATTT CTTCTTTTCA 
    TGTCAGAAGA GTCTTGAACT GCTTGTGAAC CAGAGGAACC ATGCCTGTCT GGAGAGATTT
    ACCAGCATGA GAATTAAGAA GGAGAAGGAA AAGCCCAATT CTGCTCATAG AAATTCTTCT
    GCATCATATG GGGATGATCC CACAGCACAG TCATTGCAAG ATGTTTCAGA TGAACAAGTG
    CTGGTTCTCT TTGAACAGAT GCTGCTGGAT ATGAACCTGA ATGAGGAGAA ACAGCAACCT
    TTGAGGGAGA AGGACATCAT CATCAAGAGG GAGATGGTGT CCCAATACTT GTACACCTCC
    AAGGCTGGCA TGAGCCAGAA GGAGAGCTCT AAGTCTGCCA TGATGTATAT TCAGGAGTTG
    AGGTCAGGCT TGCGGGATAT GCCTCTGCTC AGCTGCCTGG AGTCCCTTCG TGTGTCTCTC
    AACAACAACC CTGTCAGTTG GGTGCAAACA TTTGGTGCTG AAGGCTTGGC CTCCTTATTG
    GACATTCTTA AACGACTTCA TGATGAGAAA GAAGAGACTG CTGGGAGTTA CGATAGCCGG
    AACAAGCATG AGATCATTCG CTGCTTGAAA GCTTTTATGA ACAACAAGTT TGGAATCAAG
    ACCATGTTGG AGACAGAAGA AGGAATCCTA CTGCTGGTCA GAGCCATGGA TCCTGCTGTT
    CCCAACATGA TGATTGATGC AGCTAAGCTG CTTTCTGCTC TTTGTATTCT ACCGCAGCCA
    GAGGACATGA ATGAAAGGGT TTTGGAGGCA ATGACAGAAA GAGCTGAGAT GGATGAAGTG
    GAACGTTTCC AGCCGCTGCT GGATGGATTA AAAAGTGGAA CCACTATTGC ACTGAAGGTT
    GGATGCCTAC AGCTGATCAA TGCTCTCATC ACACCAGCGG AGGAACTTGA CTTCCGAGTT
    CACATCAGAA GTGAACTGAT GCGTTTGGGG CTACATCAGG TGTTGCAGGA CCTTCGAGAG
    ATTGAAAATG AAGATATGAG AGTGCAACTA AATGTGTTTG ATGAACAAGG GGAAGAGGAT
    TCCTATGACC TGAAGGGACG GCTGGATGAC ATTCGCATGG AGATGGATGA CTTTAATGAA
    GTCTTTCAGA TTCTCTTAAA CACAGTGAAG GATTCAAAGG CAGAGCCACA CTTCCTTTCC
    ATCCTGCAGC ACTTACTCTT GGTCCGAAAT GACTATGAGG CCAGACCTCA GTACTATAAG
    TTGATTGAAG AATGTATTTC CCAGATAGTT CTGCACAAGA ACGGGGCTGA TCCTGACTTC
    AAGTGCCGGC ACCTCCAGAT TGAGATTGAG GGATTAATTG ATCAAATGAT TGATAAGACA
    AAGGTGGAGA AATCTGAAGC CAAAGCTGCA GAGCTGGAAA AGAAGTTGGA CTCAGAGTTA
    ACAGCCCGAC ATGAGCTACA GGTGGAAATG AAAAAGATGG AAAGTGACTT TGAGCAGAAG
    CTTCAAGATC TTCAGGGAGA AAAAGATGCA CTGCATTCTG AAAAGCAGCA AATTGCCACA
    GAGAAACAGG ACCTGGAAGC AGAGGTGTCC CAGCTCACAG GAGAGGTTGC CAAGCTGACA
    AAGGAACTGG AAGATGCCAA GAAAGAAATG GCTTCCCTCT CTGCGGCAGC TATTACTGTA
    CCTCCTTCTG TTCCTAGTCG TGCTCCTGTT CCCCCTGCCC CTCCTTTACC TGGTGACTCT
    GGCACTATTA TTCCACCACC ACCTGCTCCT GGGGATAGTA CCACTCCTCC TCCTCCTCCT
    CCTCCTCCTC CTCCTCCACC TCCTTTGCCT GGGGGTGTTT GCATCTCCTC ACCCCCTTCT
    TTACCTGGAG GTACTGCTAT CTCTCCACCC CCTCCTTTGT CTGGGGATGC TACCATCCCT
    CCACCCCCTC CTTTGCCTGA GGGTGTTGGC ATCCCTTCAC CCTCTTCTTT GCCTGGAGGT
    ACTGCCATCC CCCCACCTCC TCCTTTGCCT GGGAGTGCTA GAATCCCCCC ACCACCACCT
    CCTTTGCCTG GGAGTGCTGG AATTCCCCCC CCACCTCCTC CCTTGCCTGG AGAAGCAGGA
    ATGCCACCTC CTCCTCCCCC TCTTCCTGGT GGTCCTGGAA TCCCTCCACC TCCTCCATTT
    CCCGGAGGCC CTGGCATTCC TCCACCTCCA CCCGGAATGG GTATGCCTCC ACCTCCCCCA
    TTTGGATTTG GAGTTCCTGC AGCCCCAGTT CTGCCATTTG GATTAACCCC CAAAAAGCTT
    TATAAGCCAG AGGTGCAGCT CCGGAGGCCA AACTGGTCCA AGCTTGTGGC TGAGGACCTC
    TCCCAGGACT GCTTCTGGAC AAAGGTGAAG GAGGACCGCT TTGAGAACAA TGAACTTTTC
    GCCAAACTTA CCCTTACCTT CTCTGCCCAG ACCAAGACTT CCAAAGCCAA GAAGGATCAA
    GAAGGTGGAG AAGAAAAGAA ATCTGTGCAA AAGAAAAAAG TAAAAGAGTT AAAGGTGTTG
    GATTCAAAGA CAGCCCAGAA TCTCTCAATC TTTTTGGGTT CCTTCCGCAT GCCCTATCAA
    GAGATTAAGA ATGTCATCCT GGAGGTGAAT GAGGCTGTTC TGACTGAGTC TATGATCCAG
    AACCTCATTA AGCAAATGCC AGAGCCAGAG CAGTTAAAAA TGCTTTCTGA ACTGAAGGAT
    GAATATGATG ACCTGGCTGA GTCAGAGCAG TTTGGCGTGG TGATGGGCAC TGTGCCCCGA
    CTGCGGCCTC GCCTCAATGC CATTCTCTTC AAGCTACAAT TCAGCGAGCA AGTGGAGAAT
    ATCAAGCCAG AGATTGTGTC TGTCACTGCT GCATGTGAGG AGTTACGTAA GAGTGAGAGC
    TTTTCCAATC TCCTAGAGAT TACCTTGCTT GTTGGAAATT ACATGAATGC TGGCTCCAGA
    AATGCTGGTG CTTTTGGCTT CAATATCAGC TTCCTCTGTA AGCTTCGAGA CACCAAGTCC
    ACAGATCAGA AGATGACGTT GTTACACTTC TTGGCTGAGT TGTGTGAGAA TGACTATCCC
    GATGTCCTCA AGTTTCCAGA CGAGCTTGCC CATGTGGAGA AAGCCAGCCG AGTTTCTGCT
    GAAAACTTGC AAAAGAACCT AGATCAGATG AAGAAACAAA TTTCTGATGT GGAACGTGAT
    GTTCAGAATT TCCCAGCTGC CACAGATGAA AAAGACAAGT TTGTTGAAAA AATGACCAGC
    TTTGTGAAGG ATGCACAGGA ACAGTATAAC AAGCTGCGGA TGATGCATTC TAACATGGAG
    ACCCTCTATA AGGAGCTGGG CGAGTACTTC CTCTTTGACC CCAAGAAGTT GTCTGTTGAA
    GAATTTTTCA TGGATCTTCA CAATTTTCGG AATATGTTTT TGCAAGCAGT CAAGGAGAAC
    CAGAAGCGGC GGGAGACAGA AGAAAAGATG AGGCGAGCAA AACTAGCCAA GGAGAAGGCA
    GAGAAGGAGC GGCTAGAGAA GCAGCAGAAG AGAGAGCAAC TCATAGACAT GAATGCAGAG
    GGCGATGAGA CAGGTGTGAT GGACAGTCTT CTAGAAGCCC TGCAGTCAGG GGCAGCATTC
    CGACGGAAGA GAGGGCCCCG TCAAGCCAAC AGGAAGGCCG GGTGTGCAGT CACATCTCTG
    CTAGCTTCGG AGCTGACCAA GGATGATGCC ATGGCTGCTG TTCCTGCCAA GGTGTCCAAG
    AACAGTGAGA CATTCCCCAC AATCCTTGAG GAAGCCAAGG AGTTGGTTGG CCGTGCAAGC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011535874.1
    CDS119..3901
    Translation

    Target ORF information:

    RefSeq Version XM_011537572.2
    Organism Homo sapiens(human)
    Definition Homo sapiens diaphanous related formin 1 (DIAPH1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011537572.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGAGAGTAC AGAGTCAGTA TGGTAAGTTA GATAGGAGGA AGACTGATTT CTTCTTTTCA 
    TGTCAGAAGA GTCTTGAACT GCTTGTGAAC CAGAGGAACC ATGCCTGTCT GGAGAGATTT
    ACCAGCATGA GAATTAAGAA GGAGAAGGAA AAGCCCAATT CTGCTCATAG AAATTCTTCT
    GCATCATATG GGGATGATCC CACAGCACAG TCATTGCAAG ATGTTTCAGA TGAACAAGTG
    CTGGTTCTCT TTGAACAGAT GCTGCTGGAT ATGAACCTGA ATGAGGAGAA ACAGCAACCT
    TTGAGGGAGA AGGACATCAT CATCAAGAGG GAGATGGTGT CCCAATACTT GTACACCTCC
    AAGGCTGGCA TGAGCCAGAA GGAGAGCTCT AAGTCTGCCA TGATGTATAT TCAGGAGTTG
    AGGTCAGGCT TGCGGGATAT GCCTCTGCTC AGCTGCCTGG AGTCCCTTCG TGTGTCTCTC
    AACAACAACC CTGTCAGTTG GGTGCAAACA TTTGGTGCTG AAGGCTTGGC CTCCTTATTG
    GACATTCTTA AACGACTTCA TGATGAGAAA GAAGAGACTG CTGGGAGTTA CGATAGCCGG
    AACAAGCATG AGATCATTCG CTGCTTGAAA GCTTTTATGA ACAACAAGTT TGGAATCAAG
    ACCATGTTGG AGACAGAAGA AGGAATCCTA CTGCTGGTCA GAGCCATGGA TCCTGCTGTT
    CCCAACATGA TGATTGATGC AGCTAAGCTG CTTTCTGCTC TTTGTATTCT ACCGCAGCCA
    GAGGACATGA ATGAAAGGGT TTTGGAGGCA ATGACAGAAA GAGCTGAGAT GGATGAAGTG
    GAACGTTTCC AGCCGCTGCT GGATGGATTA AAAAGTGGAA CCACTATTGC ACTGAAGGTT
    GGATGCCTAC AGCTGATCAA TGCTCTCATC ACACCAGCGG AGGAACTTGA CTTCCGAGTT
    CACATCAGAA GTGAACTGAT GCGTTTGGGG CTACATCAGG TGTTGCAGGA CCTTCGAGAG
    ATTGAAAATG AAGATATGAG AGTGCAACTA AATGTGTTTG ATGAACAAGG GGAAGAGGAT
    TCCTATGACC TGAAGGGACG GCTGGATGAC ATTCGCATGG AGATGGATGA CTTTAATGAA
    GTCTTTCAGA TTCTCTTAAA CACAGTGAAG GATTCAAAGG CAGAGCCACA CTTCCTTTCC
    ATCCTGCAGC ACTTACTCTT GGTCCGAAAT GACTATGAGG CCAGACCTCA GTACTATAAG
    TTGATTGAAG AATGTATTTC CCAGATAGTT CTGCACAAGA ACGGGGCTGA TCCTGACTTC
    AAGTGCCGGC ACCTCCAGAT TGAGATTGAG GGATTAATTG ATCAAATGAT TGATAAGACA
    AAGGTGGAGA AATCTGAAGC CAAAGCTGCA GAGCTGGAAA AGAAGTTGGA CTCAGAGTTA
    ACAGCCCGAC ATGAGCTACA GGTGGAAATG AAAAAGATGG AAAGTGACTT TGAGCAGAAG
    CTTCAAGATC TTCAGGGAGA AAAAGATGCA CTGCATTCTG AAAAGCAGCA AATTGCCACA
    GAGAAACAGG ACCTGGAAGC AGAGGTGTCC CAGCTCACAG GAGAGGTTGC CAAGCTGACA
    AAGGAACTGG AAGATGCCAA GAAAGAAATG GCTTCCCTCT CTGCGGCAGC TATTACTGTA
    CCTCCTTCTG TTCCTAGTCG TGCTCCTGTT CCCCCTGCCC CTCCTTTACC TGGTGACTCT
    GGCACTATTA TTCCACCACC ACCTGCTCCT GGGGATAGTA CCACTCCTCC TCCTCCTCCT
    CCTCCTCCTC CTCCTCCACC TCCTTTGCCT GGGGGTGTTT GCATCTCCTC ACCCCCTTCT
    TTACCTGGAG GTACTGCTAT CTCTCCACCC CCTCCTTTGT CTGGGGATGC TACCATCCCT
    CCACCCCCTC CTTTGCCTGA GGGTGTTGGC ATCCCTTCAC CCTCTTCTTT GCCTGGAGGT
    ACTGCCATCC CCCCACCTCC TCCTTTGCCT GGGAGTGCTA GAATCCCCCC ACCACCACCT
    CCTTTGCCTG GGAGTGCTGG AATTCCCCCC CCACCTCCTC CCTTGCCTGG AGAAGCAGGA
    ATGCCACCTC CTCCTCCCCC TCTTCCTGGT GGTCCTGGAA TCCCTCCACC TCCTCCATTT
    CCCGGAGGCC CTGGCATTCC TCCACCTCCA CCCGGAATGG GTATGCCTCC ACCTCCCCCA
    TTTGGATTTG GAGTTCCTGC AGCCCCAGTT CTGCCATTTG GATTAACCCC CAAAAAGCTT
    TATAAGCCAG AGGTGCAGCT CCGGAGGCCA AACTGGTCCA AGCTTGTGGC TGAGGACCTC
    TCCCAGGACT GCTTCTGGAC AAAGGTGAAG GAGGACCGCT TTGAGAACAA TGAACTTTTC
    GCCAAACTTA CCCTTACCTT CTCTGCCCAG ACCAAGACTT CCAAAGCCAA GAAGGATCAA
    GAAGGTGGAG AAGAAAAGAA ATCTGTGCAA AAGAAAAAAG TAAAAGAGTT AAAGGTGTTG
    GATTCAAAGA CAGCCCAGAA TCTCTCAATC TTTTTGGGTT CCTTCCGCAT GCCCTATCAA
    GAGATTAAGA ATGTCATCCT GGAGGTGAAT GAGGCTGTTC TGACTGAGTC TATGATCCAG
    AACCTCATTA AGCAAATGCC AGAGCCAGAG CAGTTAAAAA TGCTTTCTGA ACTGAAGGAT
    GAATATGATG ACCTGGCTGA GTCAGAGCAG TTTGGCGTGG TGATGGGCAC TGTGCCCCGA
    CTGCGGCCTC GCCTCAATGC CATTCTCTTC AAGCTACAAT TCAGCGAGCA AGTGGAGAAT
    ATCAAGCCAG AGATTGTGTC TGTCACTGCT GCATGTGAGG AGTTACGTAA GAGTGAGAGC
    TTTTCCAATC TCCTAGAGAT TACCTTGCTT GTTGGAAATT ACATGAATGC TGGCTCCAGA
    AATGCTGGTG CTTTTGGCTT CAATATCAGC TTCCTCTGTA AGCTTCGAGA CACCAAGTCC
    ACAGATCAGA AGATGACGTT GTTACACTTC TTGGCTGAGT TGTGTGAGAA TGACTATCCC
    GATGTCCTCA AGTTTCCAGA CGAGCTTGCC CATGTGGAGA AAGCCAGCCG AGTTTCTGCT
    GAAAACTTGC AAAAGAACCT AGATCAGATG AAGAAACAAA TTTCTGATGT GGAACGTGAT
    GTTCAGAATT TCCCAGCTGC CACAGATGAA AAAGACAAGT TTGTTGAAAA AATGACCAGC
    TTTGTGAAGG ATGCACAGGA ACAGTATAAC AAGCTGCGGA TGATGCATTC TAACATGGAG
    ACCCTCTATA AGGAGCTGGG CGAGTACTTC CTCTTTGACC CCAAGAAGTT GTCTGTTGAA
    GAATTTTTCA TGGATCTTCA CAATTTTCGG AATATGTTTT TGCAAGCAGT CAAGGAGAAC
    CAGAAGCGGC GGGAGACAGA AGAAAAGATG AGGCGAGCAA AACTAGCCAA GGAGAAGGCA
    GAGAAGGAGC GGCTAGAGAA GCAGCAGAAG AGAGAGCAAC TCATAGACAT GAATGCAGAG
    GGCGATGAGA CAGGTGTGAT GGACAGTCTT CTAGAAGCCC TGCAGTCAGG GGCAGCATTC
    CGACGGAAGA GAGGGCCCCG TCAAGCCAAC AGGAAGGCCG GGTGTGCAGT CACATCTCTG
    CTAGCTTCGG AGCTGACCAA GGATGATGCC ATGGCTGCTG TTCCTGCCAA GGTGTCCAAG
    AACAGTGAGA CATTCCCCAC AATCCTTGAG GAAGCCAAGG AGTTGGTTGG CCGTGCAAGC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu57239
    Clone ID Related Accession (Same CDS sequence) XM_011537573.2
    Accession Version XM_011537573.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3753bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product protein diaphanous homolog 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011537573.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGTGATAA ACCTCTCCTG TGGGAAGAAA GTTGGACTGC GAGTTCAAGA AACTCTGAAG 
    CGGCTCATGG CAGATGAGCT GGAGAGATTT ACCAGCATGA GAATTAAGAA GGAGAAGGAA
    AAGCCCAATT CTGCTCATAG AAATTCTTCT GCATCATATG GGGATGATCC CACAGCACAG
    TCATTGCAAG ATGTTTCAGA TGAACAAGTG CTGGTTCTCT TTGAACAGAT GCTGCTGGAT
    ATGAACCTGA ATGAGGAGAA ACAGCAACCT TTGAGGGAGA AGGACATCAT CATCAAGAGG
    GAGATGGTGT CCCAATACTT GTACACCTCC AAGGCTGGCA TGAGCCAGAA GGAGAGCTCT
    AAGTCTGCCA TGATGTATAT TCAGGAGTTG AGGTCAGGCT TGCGGGATAT GCCTCTGCTC
    AGCTGCCTGG AGTCCCTTCG TGTGTCTCTC AACAACAACC CTGTCAGTTG GGTGCAAACA
    TTTGGTGCTG AAGGCTTGGC CTCCTTATTG GACATTCTTA AACGACTTCA TGATGAGAAA
    GAAGAGACTG CTGGGAGTTA CGATAGCCGG AACAAGCATG AGATCATTCG CTGCTTGAAA
    GCTTTTATGA ACAACAAGTT TGGAATCAAG ACCATGTTGG AGACAGAAGA AGGAATCCTA
    CTGCTGGTCA GAGCCATGGA TCCTGCTGTT CCCAACATGA TGATTGATGC AGCTAAGCTG
    CTTTCTGCTC TTTGTATTCT ACCGCAGCCA GAGGACATGA ATGAAAGGGT TTTGGAGGCA
    ATGACAGAAA GAGCTGAGAT GGATGAAGTG GAACGTTTCC AGCCGCTGCT GGATGGATTA
    AAAAGTGGAA CCACTATTGC ACTGAAGGTT GGATGCCTAC AGCTGATCAA TGCTCTCATC
    ACACCAGCGG AGGAACTTGA CTTCCGAGTT CACATCAGAA GTGAACTGAT GCGTTTGGGG
    CTACATCAGG TGTTGCAGGA CCTTCGAGAG ATTGAAAATG AAGATATGAG AGTGCAACTA
    AATGTGTTTG ATGAACAAGG GGAAGAGGAT TCCTATGACC TGAAGGGACG GCTGGATGAC
    ATTCGCATGG AGATGGATGA CTTTAATGAA GTCTTTCAGA TTCTCTTAAA CACAGTGAAG
    GATTCAAAGG CAGAGCCACA CTTCCTTTCC ATCCTGCAGC ACTTACTCTT GGTCCGAAAT
    GACTATGAGG CCAGACCTCA GTACTATAAG TTGATTGAAG AATGTATTTC CCAGATAGTT
    CTGCACAAGA ACGGGGCTGA TCCTGACTTC AAGTGCCGGC ACCTCCAGAT TGAGATTGAG
    GGATTAATTG ATCAAATGAT TGATAAGACA AAGGTGGAGA AATCTGAAGC CAAAGCTGCA
    GAGCTGGAAA AGAAGTTGGA CTCAGAGTTA ACAGCCCGAC ATGAGCTACA GGTGGAAATG
    AAAAAGATGG AAAGTGACTT TGAGCAGAAG CTTCAAGATC TTCAGGGAGA AAAAGATGCA
    CTGCATTCTG AAAAGCAGCA AATTGCCACA GAGAAACAGG ACCTGGAAGC AGAGGTGTCC
    CAGCTCACAG GAGAGGTTGC CAAGCTGACA AAGGAACTGG AAGATGCCAA GAAAGAAATG
    GCTTCCCTCT CTGCGGCAGC TATTACTGTA CCTCCTTCTG TTCCTAGTCG TGCTCCTGTT
    CCCCCTGCCC CTCCTTTACC TGGTGACTCT GGCACTATTA TTCCACCACC ACCTGCTCCT
    GGGGATAGTA CCACTCCTCC TCCTCCTCCT CCTCCTCCTC CTCCTCCACC TCCTTTGCCT
    GGGGGTGTTT GCATCTCCTC ACCCCCTTCT TTACCTGGAG GTACTGCTAT CTCTCCACCC
    CCTCCTTTGT CTGGGGATGC TACCATCCCT CCACCCCCTC CTTTGCCTGA GGGTGTTGGC
    ATCCCTTCAC CCTCTTCTTT GCCTGGAGGT ACTGCCATCC CCCCACCTCC TCCTTTGCCT
    GGGAGTGCTA GAATCCCCCC ACCACCACCT CCTTTGCCTG GGAGTGCTGG AATTCCCCCC
    CCACCTCCTC CCTTGCCTGG AGAAGCAGGA ATGCCACCTC CTCCTCCCCC TCTTCCTGGT
    GGTCCTGGAA TCCCTCCACC TCCTCCATTT CCCGGAGGCC CTGGCATTCC TCCACCTCCA
    CCCGGAATGG GTATGCCTCC ACCTCCCCCA TTTGGATTTG GAGTTCCTGC AGCCCCAGTT
    CTGCCATTTG GATTAACCCC CAAAAAGCTT TATAAGCCAG AGGTGCAGCT CCGGAGGCCA
    AACTGGTCCA AGCTTGTGGC TGAGGACCTC TCCCAGGACT GCTTCTGGAC AAAGGTGAAG
    GAGGACCGCT TTGAGAACAA TGAACTTTTC GCCAAACTTA CCCTTACCTT CTCTGCCCAG
    ACCAAGACTT CCAAAGCCAA GAAGGATCAA GAAGGTGGAG AAGAAAAGAA ATCTGTGCAA
    AAGAAAAAAG TAAAAGAGTT AAAGGTGTTG GATTCAAAGA CAGCCCAGAA TCTCTCAATC
    TTTTTGGGTT CCTTCCGCAT GCCCTATCAA GAGATTAAGA ATGTCATCCT GGAGGTGAAT
    GAGGCTGTTC TGACTGAGTC TATGATCCAG AACCTCATTA AGCAAATGCC AGAGCCAGAG
    CAGTTAAAAA TGCTTTCTGA ACTGAAGGAT GAATATGATG ACCTGGCTGA GTCAGAGCAG
    TTTGGCGTGG TGATGGGCAC TGTGCCCCGA CTGCGGCCTC GCCTCAATGC CATTCTCTTC
    AAGCTACAAT TCAGCGAGCA AGTGGAGAAT ATCAAGCCAG AGATTGTGTC TGTCACTGCT
    GCATGTGAGG AGTTACGTAA GAGTGAGAGC TTTTCCAATC TCCTAGAGAT TACCTTGCTT
    GTTGGAAATT ACATGAATGC TGGCTCCAGA AATGCTGGTG CTTTTGGCTT CAATATCAGC
    TTCCTCTGTA AGCTTCGAGA CACCAAGTCC ACAGATCAGA AGATGACGTT GTTACACTTC
    TTGGCTGAGT TGTGTGAGAA TGACTATCCC GATGTCCTCA AGTTTCCAGA CGAGCTTGCC
    CATGTGGAGA AAGCCAGCCG AGTTTCTGCT GAAAACTTGC AAAAGAACCT AGATCAGATG
    AAGAAACAAA TTTCTGATGT GGAACGTGAT GTTCAGAATT TCCCAGCTGC CACAGATGAA
    AAAGACAAGT TTGTTGAAAA AATGACCAGC TTTGTGAAGG ATGCACAGGA ACAGTATAAC
    AAGCTGCGGA TGATGCATTC TAACATGGAG ACCCTCTATA AGGAGCTGGG CGAGTACTTC
    CTCTTTGACC CCAAGAAGTT GTCTGTTGAA GAATTTTTCA TGGATCTTCA CAATTTTCGG
    AATATGTTTT TGCAAGCAGT CAAGGAGAAC CAGAAGCGGC GGGAGACAGA AGAAAAGATG
    AGGCGAGCAA AACTAGCCAA GGAGAAGGCA GAGAAGGAGC GGCTAGAGAA GCAGCAGAAG
    AGAGAGCAAC TCATAGACAT GAATGCAGAG GGCGATGAGA CAGGTGTGAT GGACAGTCTT
    CTAGAAGCCC TGCAGTCAGG GGCAGCATTC CGACGGAAGA GAGGGCCCCG TCAAGCCAAC
    AGGAAGGCCG GGTGTGCAGT CACATCTCTG CTAGCTTCGG AGCTGACCAA GGATGATGCC
    ATGGCTGCTG TTCCTGCCAA GGTGTCCAAG AACAGTGAGA CATTCCCCAC AATCCTTGAG
    GAAGCCAAGG AGTTGGTTGG CCGTGCAAGC TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011535875.1
    CDS16..3768
    Translation

    Target ORF information:

    RefSeq Version XM_011537573.2
    Organism Homo sapiens(human)
    Definition Homo sapiens diaphanous related formin 1 (DIAPH1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011537573.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGTGATAA ACCTCTCCTG TGGGAAGAAA GTTGGACTGC GAGTTCAAGA AACTCTGAAG 
    CGGCTCATGG CAGATGAGCT GGAGAGATTT ACCAGCATGA GAATTAAGAA GGAGAAGGAA
    AAGCCCAATT CTGCTCATAG AAATTCTTCT GCATCATATG GGGATGATCC CACAGCACAG
    TCATTGCAAG ATGTTTCAGA TGAACAAGTG CTGGTTCTCT TTGAACAGAT GCTGCTGGAT
    ATGAACCTGA ATGAGGAGAA ACAGCAACCT TTGAGGGAGA AGGACATCAT CATCAAGAGG
    GAGATGGTGT CCCAATACTT GTACACCTCC AAGGCTGGCA TGAGCCAGAA GGAGAGCTCT
    AAGTCTGCCA TGATGTATAT TCAGGAGTTG AGGTCAGGCT TGCGGGATAT GCCTCTGCTC
    AGCTGCCTGG AGTCCCTTCG TGTGTCTCTC AACAACAACC CTGTCAGTTG GGTGCAAACA
    TTTGGTGCTG AAGGCTTGGC CTCCTTATTG GACATTCTTA AACGACTTCA TGATGAGAAA
    GAAGAGACTG CTGGGAGTTA CGATAGCCGG AACAAGCATG AGATCATTCG CTGCTTGAAA
    GCTTTTATGA ACAACAAGTT TGGAATCAAG ACCATGTTGG AGACAGAAGA AGGAATCCTA
    CTGCTGGTCA GAGCCATGGA TCCTGCTGTT CCCAACATGA TGATTGATGC AGCTAAGCTG
    CTTTCTGCTC TTTGTATTCT ACCGCAGCCA GAGGACATGA ATGAAAGGGT TTTGGAGGCA
    ATGACAGAAA GAGCTGAGAT GGATGAAGTG GAACGTTTCC AGCCGCTGCT GGATGGATTA
    AAAAGTGGAA CCACTATTGC ACTGAAGGTT GGATGCCTAC AGCTGATCAA TGCTCTCATC
    ACACCAGCGG AGGAACTTGA CTTCCGAGTT CACATCAGAA GTGAACTGAT GCGTTTGGGG
    CTACATCAGG TGTTGCAGGA CCTTCGAGAG ATTGAAAATG AAGATATGAG AGTGCAACTA
    AATGTGTTTG ATGAACAAGG GGAAGAGGAT TCCTATGACC TGAAGGGACG GCTGGATGAC
    ATTCGCATGG AGATGGATGA CTTTAATGAA GTCTTTCAGA TTCTCTTAAA CACAGTGAAG
    GATTCAAAGG CAGAGCCACA CTTCCTTTCC ATCCTGCAGC ACTTACTCTT GGTCCGAAAT
    GACTATGAGG CCAGACCTCA GTACTATAAG TTGATTGAAG AATGTATTTC CCAGATAGTT
    CTGCACAAGA ACGGGGCTGA TCCTGACTTC AAGTGCCGGC ACCTCCAGAT TGAGATTGAG
    GGATTAATTG ATCAAATGAT TGATAAGACA AAGGTGGAGA AATCTGAAGC CAAAGCTGCA
    GAGCTGGAAA AGAAGTTGGA CTCAGAGTTA ACAGCCCGAC ATGAGCTACA GGTGGAAATG
    AAAAAGATGG AAAGTGACTT TGAGCAGAAG CTTCAAGATC TTCAGGGAGA AAAAGATGCA
    CTGCATTCTG AAAAGCAGCA AATTGCCACA GAGAAACAGG ACCTGGAAGC AGAGGTGTCC
    CAGCTCACAG GAGAGGTTGC CAAGCTGACA AAGGAACTGG AAGATGCCAA GAAAGAAATG
    GCTTCCCTCT CTGCGGCAGC TATTACTGTA CCTCCTTCTG TTCCTAGTCG TGCTCCTGTT
    CCCCCTGCCC CTCCTTTACC TGGTGACTCT GGCACTATTA TTCCACCACC ACCTGCTCCT
    GGGGATAGTA CCACTCCTCC TCCTCCTCCT CCTCCTCCTC CTCCTCCACC TCCTTTGCCT
    GGGGGTGTTT GCATCTCCTC ACCCCCTTCT TTACCTGGAG GTACTGCTAT CTCTCCACCC
    CCTCCTTTGT CTGGGGATGC TACCATCCCT CCACCCCCTC CTTTGCCTGA GGGTGTTGGC
    ATCCCTTCAC CCTCTTCTTT GCCTGGAGGT ACTGCCATCC CCCCACCTCC TCCTTTGCCT
    GGGAGTGCTA GAATCCCCCC ACCACCACCT CCTTTGCCTG GGAGTGCTGG AATTCCCCCC
    CCACCTCCTC CCTTGCCTGG AGAAGCAGGA ATGCCACCTC CTCCTCCCCC TCTTCCTGGT
    GGTCCTGGAA TCCCTCCACC TCCTCCATTT CCCGGAGGCC CTGGCATTCC TCCACCTCCA
    CCCGGAATGG GTATGCCTCC ACCTCCCCCA TTTGGATTTG GAGTTCCTGC AGCCCCAGTT
    CTGCCATTTG GATTAACCCC CAAAAAGCTT TATAAGCCAG AGGTGCAGCT CCGGAGGCCA
    AACTGGTCCA AGCTTGTGGC TGAGGACCTC TCCCAGGACT GCTTCTGGAC AAAGGTGAAG
    GAGGACCGCT TTGAGAACAA TGAACTTTTC GCCAAACTTA CCCTTACCTT CTCTGCCCAG
    ACCAAGACTT CCAAAGCCAA GAAGGATCAA GAAGGTGGAG AAGAAAAGAA ATCTGTGCAA
    AAGAAAAAAG TAAAAGAGTT AAAGGTGTTG GATTCAAAGA CAGCCCAGAA TCTCTCAATC
    TTTTTGGGTT CCTTCCGCAT GCCCTATCAA GAGATTAAGA ATGTCATCCT GGAGGTGAAT
    GAGGCTGTTC TGACTGAGTC TATGATCCAG AACCTCATTA AGCAAATGCC AGAGCCAGAG
    CAGTTAAAAA TGCTTTCTGA ACTGAAGGAT GAATATGATG ACCTGGCTGA GTCAGAGCAG
    TTTGGCGTGG TGATGGGCAC TGTGCCCCGA CTGCGGCCTC GCCTCAATGC CATTCTCTTC
    AAGCTACAAT TCAGCGAGCA AGTGGAGAAT ATCAAGCCAG AGATTGTGTC TGTCACTGCT
    GCATGTGAGG AGTTACGTAA GAGTGAGAGC TTTTCCAATC TCCTAGAGAT TACCTTGCTT
    GTTGGAAATT ACATGAATGC TGGCTCCAGA AATGCTGGTG CTTTTGGCTT CAATATCAGC
    TTCCTCTGTA AGCTTCGAGA CACCAAGTCC ACAGATCAGA AGATGACGTT GTTACACTTC
    TTGGCTGAGT TGTGTGAGAA TGACTATCCC GATGTCCTCA AGTTTCCAGA CGAGCTTGCC
    CATGTGGAGA AAGCCAGCCG AGTTTCTGCT GAAAACTTGC AAAAGAACCT AGATCAGATG
    AAGAAACAAA TTTCTGATGT GGAACGTGAT GTTCAGAATT TCCCAGCTGC CACAGATGAA
    AAAGACAAGT TTGTTGAAAA AATGACCAGC TTTGTGAAGG ATGCACAGGA ACAGTATAAC
    AAGCTGCGGA TGATGCATTC TAACATGGAG ACCCTCTATA AGGAGCTGGG CGAGTACTTC
    CTCTTTGACC CCAAGAAGTT GTCTGTTGAA GAATTTTTCA TGGATCTTCA CAATTTTCGG
    AATATGTTTT TGCAAGCAGT CAAGGAGAAC CAGAAGCGGC GGGAGACAGA AGAAAAGATG
    AGGCGAGCAA AACTAGCCAA GGAGAAGGCA GAGAAGGAGC GGCTAGAGAA GCAGCAGAAG
    AGAGAGCAAC TCATAGACAT GAATGCAGAG GGCGATGAGA CAGGTGTGAT GGACAGTCTT
    CTAGAAGCCC TGCAGTCAGG GGCAGCATTC CGACGGAAGA GAGGGCCCCG TCAAGCCAAC
    AGGAAGGCCG GGTGTGCAGT CACATCTCTG CTAGCTTCGG AGCTGACCAA GGATGATGCC
    ATGGCTGCTG TTCCTGCCAA GGTGTCCAAG AACAGTGAGA CATTCCCCAC AATCCTTGAG
    GAAGCCAAGG AGTTGGTTGG CCGTGCAAGC TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.