DIAPH1 gene cDNA ORF clone, Homo sapiens(human)

mRNA	Protein	Name
XM_011537572.2	XP_011535874.1	protein diaphanous homolog 1 isoform X1
NM_001079812.2	NP_001073280.1	protein diaphanous homolog 1 isoform 2
XM_024454385.1	XP_024310153.1	protein diaphanous homolog 1 isoform X2
NM_005219.4	NP_005210.3	protein diaphanous homolog 1 isoform 1
NM_001314007.1	NP_001300936.1	protein diaphanous homolog 1 isoform 3
NM_005219.5	NP_005210.3	protein diaphanous homolog 1 isoform 1
XM_024454384.1	XP_024310152.1	protein diaphanous homolog 1 isoform X1
NM_001314007.2	NP_001300936.1	protein diaphanous homolog 1 isoform 3
XM_024454386.1	XP_024310154.1	protein diaphanous homolog 1 isoform X3
XM_011537573.2	XP_011535875.1	protein diaphanous homolog 1 isoform X2
NM_001079812.3	NP_001073280.1	protein diaphanous homolog 1 isoform 2
XM_024454387.1	XP_024310155.1	protein diaphanous homolog 1 isoform X4

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu26409	NM_005219.5 Latest version!	Homo sapiens diaphanous related formin 1 (DIAPH1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$274.50-$384.30 $549.00
OHu105637	XM_024454384.1 Latest version!	Homo sapiens diaphanous related formin 1 (DIAPH1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OHu105638	XM_024454385.1 Latest version!	Homo sapiens diaphanous related formin 1 (DIAPH1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OHu105639	XM_024454386.1 Latest version!	Homo sapiens diaphanous related formin 1 (DIAPH1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OHu105640	XM_024454387.1 Latest version!	Homo sapiens diaphanous related formin 1 (DIAPH1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OHu26133	NM_001079812.3 Latest version!	Homo sapiens diaphanous related formin 1 (DIAPH1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$384.30 $549.00
OHu39824	NM_001314007.2 Latest version!	Homo sapiens diaphanous related formin 1 (DIAPH1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$643.30 $919.00
OHu57238	XM_011537572.2 Latest version!	Homo sapiens diaphanous related formin 1 (DIAPH1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$643.30 $919.00
OHu57239	XM_011537573.2 Latest version!	Homo sapiens diaphanous related formin 1 (DIAPH1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$643.30 $919.00
View Old Accession Versions >>
OHu26409	NM_005219.4	Homo sapiens diaphanous related formin 1 (DIAPH1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$274.50-$384.30 $549.00
OHu39824	NM_001314007.1	Homo sapiens diaphanous related formin 1 (DIAPH1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$643.30 $919.00
OHu26133	NM_001079812.2	Homo sapiens diaphanous related formin 1 (DIAPH1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$384.30 $549.00
Hide Old Accession Versions >>

DIAPH1 ( NM_005219.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_005210.3 Homo sapiens diaphanous related formin 1 (DIAPH1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu26409
Clone ID Related Accession (Same CDS sequence)	NM_005219.4 , NM_005219.5
Accession Version	NM_005219.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3819bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-05
Organism	Homo sapiens(human)
Product	protein diaphanous homolog 1 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA593734.1, AB209482.1, AY363395.1 and AY007129.1. This sequence is a reference standard in the RefSeqGene project. On May 24, 2008 this sequence version replaced NM_005219.3. Transcript Variant: This variant (1) encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB209482.1, BC143413.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGCCGC CCGGCGGGAG CCTGGGGCCC GGCCGCGGGA CCCGGGACAA GAAGAAGGGC 
CGGAGCCCAG ATGAGCTGCC CTCGGCGGGC GGCGACGGCG GCAAATCTAA GAAATTTACT 
CTGAAGCGGC TCATGGCAGA TGAGCTGGAG AGATTTACCA GCATGAGAAT TAAGAAGGAG 
AAGGAAAAGC CCAATTCTGC TCATAGAAAT TCTTCTGCAT CATATGGGGA TGATCCCACA 
GCACAGTCAT TGCAAGATGT TTCAGATGAA CAAGTGCTGG TTCTCTTTGA ACAGATGCTG 
CTGGATATGA ACCTGAATGA GGAGAAACAG CAACCTTTGA GGGAGAAGGA CATCATCATC 
AAGAGGGAGA TGGTGTCCCA ATACTTGTAC ACCTCCAAGG CTGGCATGAG CCAGAAGGAG 
AGCTCTAAGT CTGCCATGAT GTATATTCAG GAGTTGAGGT CAGGCTTGCG GGATATGCCT 
CTGCTCAGCT GCCTGGAGTC CCTTCGTGTG TCTCTCAACA ACAACCCTGT CAGTTGGGTG 
CAAACATTTG GTGCTGAAGG CTTGGCCTCC TTATTGGACA TTCTTAAACG ACTTCATGAT 
GAGAAAGAAG AGACTGCTGG GAGTTACGAT AGCCGGAACA AGCATGAGAT CATTCGCTGC 
TTGAAAGCTT TTATGAACAA CAAGTTTGGA ATCAAGACCA TGTTGGAGAC AGAAGAAGGA 
ATCCTACTGC TGGTCAGAGC CATGGATCCT GCTGTTCCCA ACATGATGAT TGATGCAGCT 
AAGCTGCTTT CTGCTCTTTG TATTCTACCG CAGCCAGAGG ACATGAATGA AAGGGTTTTG 
GAGGCAATGA CAGAAAGAGC TGAGATGGAT GAAGTGGAAC GTTTCCAGCC GCTGCTGGAT 
GGATTAAAAA GTGGAACCAC TATTGCACTG AAGGTTGGAT GCCTACAGCT GATCAATGCT 
CTCATCACAC CAGCGGAGGA ACTTGACTTC CGAGTTCACA TCAGAAGTGA ACTGATGCGT 
TTGGGGCTAC ATCAGGTGTT GCAGGACCTT CGAGAGATTG AAAATGAAGA TATGAGAGTG 
CAACTAAATG TGTTTGATGA ACAAGGGGAA GAGGATTCCT ATGACCTGAA GGGACGGCTG 
GATGACATTC GCATGGAGAT GGATGACTTT AATGAAGTCT TTCAGATTCT CTTAAACACA 
GTGAAGGATT CAAAGGCAGA GCCACACTTC CTTTCCATCC TGCAGCACTT ACTCTTGGTC 
CGAAATGACT ATGAGGCCAG ACCTCAGTAC TATAAGTTGA TTGAAGAATG TATTTCCCAG 
ATAGTTCTGC ACAAGAACGG GGCTGATCCT GACTTCAAGT GCCGGCACCT CCAGATTGAG 
ATTGAGGGAT TAATTGATCA AATGATTGAT AAGACAAAGG TGGAGAAATC TGAAGCCAAA 
GCTGCAGAGC TGGAAAAGAA GTTGGACTCA GAGTTAACAG CCCGACATGA GCTACAGGTG 
GAAATGAAAA AGATGGAAAG TGACTTTGAG CAGAAGCTTC AAGATCTTCA GGGAGAAAAA 
GATGCACTGC ATTCTGAAAA GCAGCAAATT GCCACAGAGA AACAGGACCT GGAAGCAGAG 
GTGTCCCAGC TCACAGGAGA GGTTGCCAAG CTGACAAAGG AACTGGAAGA TGCCAAGAAA 
GAAATGGCTT CCCTCTCTGC GGCAGCTATT ACTGTACCTC CTTCTGTTCC TAGTCGTGCT 
CCTGTTCCCC CTGCCCCTCC TTTACCTGGT GACTCTGGCA CTATTATTCC ACCACCACCT 
GCTCCTGGGG ATAGTACCAC TCCTCCTCCT CCTCCTCCTC CTCCTCCTCC TCCACCTCCT 
TTGCCTGGGG GTGTTTGCAT CTCCTCACCC CCTTCTTTAC CTGGAGGTAC TGCTATCTCT 
CCACCCCCTC CTTTGTCTGG GGATGCTACC ATCCCTCCAC CCCCTCCTTT GCCTGAGGGT 
GTTGGCATCC CTTCACCCTC TTCTTTGCCT GGAGGTACTG CCATCCCCCC ACCTCCTCCT 
TTGCCTGGGA GTGCTAGAAT CCCCCCACCA CCACCTCCTT TGCCTGGGAG TGCTGGAATT 
CCCCCCCCAC CTCCTCCCTT GCCTGGAGAA GCAGGAATGC CACCTCCTCC TCCCCCTCTT 
CCTGGTGGTC CTGGAATCCC TCCACCTCCT CCATTTCCCG GAGGCCCTGG CATTCCTCCA 
CCTCCACCCG GAATGGGTAT GCCTCCACCT CCCCCATTTG GATTTGGAGT TCCTGCAGCC 
CCAGTTCTGC CATTTGGATT AACCCCCAAA AAGCTTTATA AGCCAGAGGT GCAGCTCCGG 
AGGCCAAACT GGTCCAAGCT TGTGGCTGAG GACCTCTCCC AGGACTGCTT CTGGACAAAG 
GTGAAGGAGG ACCGCTTTGA GAACAATGAA CTTTTCGCCA AACTTACCCT TACCTTCTCT 
GCCCAGACCA AGACTTCCAA AGCCAAGAAG GATCAAGAAG GTGGAGAAGA AAAGAAATCT 
GTGCAAAAGA AAAAAGTAAA AGAGTTAAAG GTGTTGGATT CAAAGACAGC CCAGAATCTC 
TCAATCTTTT TGGGTTCCTT CCGCATGCCC TATCAAGAGA TTAAGAATGT CATCCTGGAG 
GTGAATGAGG CTGTTCTGAC TGAGTCTATG ATCCAGAACC TCATTAAGCA AATGCCAGAG 
CCAGAGCAGT TAAAAATGCT TTCTGAACTG AAGGATGAAT ATGATGACCT GGCTGAGTCA 
GAGCAGTTTG GCGTGGTGAT GGGCACTGTG CCCCGACTGC GGCCTCGCCT CAATGCCATT 
CTCTTCAAGC TACAATTCAG CGAGCAAGTG GAGAATATCA AGCCAGAGAT TGTGTCTGTC 
ACTGCTGCAT GTGAGGAGTT ACGTAAGAGT GAGAGCTTTT CCAATCTCCT AGAGATTACC 
TTGCTTGTTG GAAATTACAT GAATGCTGGC TCCAGAAATG CTGGTGCTTT TGGCTTCAAT 
ATCAGCTTCC TCTGTAAGCT TCGAGACACC AAGTCCACAG ATCAGAAGAT GACGTTGTTA 
CACTTCTTGG CTGAGTTGTG TGAGAATGAC TATCCCGATG TCCTCAAGTT TCCAGACGAG 
CTTGCCCATG TGGAGAAAGC CAGCCGAGTT TCTGCTGAAA ACTTGCAAAA GAACCTAGAT 
CAGATGAAGA AACAAATTTC TGATGTGGAA CGTGATGTTC AGAATTTCCC AGCTGCCACA 
GATGAAAAAG ACAAGTTTGT TGAAAAAATG ACCAGCTTTG TGAAGGATGC ACAGGAACAG 
TATAACAAGC TGCGGATGAT GCATTCTAAC ATGGAGACCC TCTATAAGGA GCTGGGCGAG 
TACTTCCTCT TTGACCCCAA GAAGTTGTCT GTTGAAGAAT TTTTCATGGA TCTTCACAAT 
TTTCGGAATA TGTTTTTGCA AGCAGTCAAG GAGAACCAGA AGCGGCGGGA GACAGAAGAA 
AAGATGAGGC GAGCAAAACT AGCCAAGGAG AAGGCAGAGA AGGAGCGGCT AGAGAAGCAG 
CAGAAGAGAG AGCAACTCAT AGACATGAAT GCAGAGGGCG ATGAGACAGG TGTGATGGAC 
AGTCTTCTAG AAGCCCTGCA GTCAGGGGCA GCATTCCGAC GGAAGAGAGG GCCCCGTCAA 
GCCAACAGGA AGGCCGGGTG TGCAGTCACA TCTCTGCTAG CTTCGGAGCT GACCAAGGAT 
GATGCCATGG CTGCTGTTCC TGCCAAGGTG TCCAAGAACA GTGAGACATT CCCCACAATC 
CTTGAGGAAG CCAAGGAGTT GGTTGGCCGT GCAAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005210.3
CDS	142..3960
Translation	MEPPGGSLGPGRGTRDKKKGRSPDELPSAGGDGGKSKKFTLKRLMADELERFTSMRIKKEKEKPNSAHRNSSASYGDDPTAQSLQDVSDEQVLVLFEQMLLDMNLNEEKQQPLREKDIIIKREMVSQYLYTSKAGMSQKESSKSAMMYIQELRSGLRDMPLLSCLESLRVSLNNNPVSWVQTFGAEGLASLLDILKRLHDEKEETAGSYDSRNKHEIIRCLKAFMNNKFGIKTMLETEEGILLLVRAMDPAVPNMMIDAAKLLSALCILPQPEDMNERVLEAMTERAEMDEVERFQPLLDGLKSGTTIALKVGCLQLINALITPAEELDFRVHIRSELMRLGLHQVLQDLREIENEDMRVQLNVFDEQGEEDSYDLKGRLDDIRMEMDDFNEVFQILLNTVKDSKAEPHFLSILQHLLLVRNDYEARPQYYKLIEECISQIVLHKNGADPDFKCRHLQIEIEGLIDQMIDKTKVEKSEAKAAELEKKLDSELTARHELQVEMKKMESDFEQKLQDLQGEKDALHSEKQQIATEKQDLEAEVSQLTGEVAKLTKELEDAKKEMASLSAAAITVPPSVPSRAPVPPAPPLPGDSGTIIPPPPAPGDSTTPPPPPPPPPPPPPLPGGVCISSPPSLPGGTAISPPPPLSGDATIPPPPPLPEGVGIPSPSSLPGGTAIPPPPPLPGSARIPPPPPPLPGSAGIPPPPPPLPGEAGMPPPPPPLPGGPGIPPPPPFPGGPGIPPPPPGMGMPPPPPFGFGVPAAPVLPFGLTPKKLYKPEVQLRRPNWSKLVAEDLSQDCFWTKVKEDRFENNELFAKLTLTFSAQTKTSKAKKDQEGGEEKKSVQKKKVKELKVLDSKTAQNLSIFLGSFRMPYQEIKNVILEVNEAVLTESMIQNLIKQMPEPEQLKMLSELKDEYDDLAESEQFGVVMGTVPRLRPRLNAILFKLQFSEQVENIKPEIVSVTAACEELRKSESFSNLLEITLLVGNYMNAGSRNAGAFGFNISFLCKLRDTKSTDQKMTLLHFLAELCENDYPDVLKFPDELAHVEKASRVSAENLQKNLDQMKKQISDVERDVQNFPAATDEKDKFVEKMTSFVKDAQEQYNKLRMMHSNMETLYKELGEYFLFDPKKLSVEEFFMDLHNFRNMFLQAVKENQKRRETEEKMRRAKLAKEKAEKERLEKQQKREQLIDMNAEGDETGVMDSLLEALQSGAAFRRKRGPRQANRKAGCAVTSLLASELTKDDAMAAVPAKVSKNSETFPTILEEAKELVGRAS

Target ORF information:

RefSeq Version	NM_005219.4
Organism	Homo sapiens(human)
Definition	Homo sapiens diaphanous related formin 1 (DIAPH1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005219.4

ORF Insert Sequence:

ATGGAGCCGC CCGGCGGGAG CCTGGGGCCC GGCCGCGGGA CCCGGGACAA GAAGAAGGGC 
CGGAGCCCAG ATGAGCTGCC CTCGGCGGGC GGCGACGGCG GCAAATCTAA GAAATTTACT 
CTGAAGCGGC TCATGGCAGA TGAGCTGGAG AGATTTACCA GCATGAGAAT TAAGAAGGAG 
AAGGAAAAGC CCAATTCTGC TCATAGAAAT TCTTCTGCAT CATATGGGGA TGATCCCACA 
GCACAGTCAT TGCAAGATGT TTCAGATGAA CAAGTGCTGG TTCTCTTTGA ACAGATGCTG 
CTGGATATGA ACCTGAATGA GGAGAAACAG CAACCTTTGA GGGAGAAGGA CATCATCATC 
AAGAGGGAGA TGGTGTCCCA ATACTTGTAC ACCTCCAAGG CTGGCATGAG CCAGAAGGAG 
AGCTCTAAGT CTGCCATGAT GTATATTCAG GAGTTGAGGT CAGGCTTGCG GGATATGCCT 
CTGCTCAGCT GCCTGGAGTC CCTTCGTGTG TCTCTCAACA ACAACCCTGT CAGTTGGGTG 
CAAACATTTG GTGCTGAAGG CTTGGCCTCC TTATTGGACA TTCTTAAACG ACTTCATGAT 
GAGAAAGAAG AGACTGCTGG GAGTTACGAT AGCCGGAACA AGCATGAGAT CATTCGCTGC 
TTGAAAGCTT TTATGAACAA CAAGTTTGGA ATCAAGACCA TGTTGGAGAC AGAAGAAGGA 
ATCCTACTGC TGGTCAGAGC CATGGATCCT GCTGTTCCCA ACATGATGAT TGATGCAGCT 
AAGCTGCTTT CTGCTCTTTG TATTCTACCG CAGCCAGAGG ACATGAATGA AAGGGTTTTG 
GAGGCAATGA CAGAAAGAGC TGAGATGGAT GAAGTGGAAC GTTTCCAGCC GCTGCTGGAT 
GGATTAAAAA GTGGAACCAC TATTGCACTG AAGGTTGGAT GCCTACAGCT GATCAATGCT 
CTCATCACAC CAGCGGAGGA ACTTGACTTC CGAGTTCACA TCAGAAGTGA ACTGATGCGT 
TTGGGGCTAC ATCAGGTGTT GCAGGACCTT CGAGAGATTG AAAATGAAGA TATGAGAGTG 
CAACTAAATG TGTTTGATGA ACAAGGGGAA GAGGATTCCT ATGACCTGAA GGGACGGCTG 
GATGACATTC GCATGGAGAT GGATGACTTT AATGAAGTCT TTCAGATTCT CTTAAACACA 
GTGAAGGATT CAAAGGCAGA GCCACACTTC CTTTCCATCC TGCAGCACTT ACTCTTGGTC 
CGAAATGACT ATGAGGCCAG ACCTCAGTAC TATAAGTTGA TTGAAGAATG TATTTCCCAG 
ATAGTTCTGC ACAAGAACGG GGCTGATCCT GACTTCAAGT GCCGGCACCT CCAGATTGAG 
ATTGAGGGAT TAATTGATCA AATGATTGAT AAGACAAAGG TGGAGAAATC TGAAGCCAAA 
GCTGCAGAGC TGGAAAAGAA GTTGGACTCA GAGTTAACAG CCCGACATGA GCTACAGGTG 
GAAATGAAAA AGATGGAAAG TGACTTTGAG CAGAAGCTTC AAGATCTTCA GGGAGAAAAA 
GATGCACTGC ATTCTGAAAA GCAGCAAATT GCCACAGAGA AACAGGACCT GGAAGCAGAG 
GTGTCCCAGC TCACAGGAGA GGTTGCCAAG CTGACAAAGG AACTGGAAGA TGCCAAGAAA 
GAAATGGCTT CCCTCTCTGC GGCAGCTATT ACTGTACCTC CTTCTGTTCC TAGTCGTGCT 
CCTGTTCCCC CTGCCCCTCC TTTACCTGGT GACTCTGGCA CTATTATTCC ACCACCACCT 
GCTCCTGGGG ATAGTACCAC TCCTCCTCCT CCTCCTCCTC CTCCTCCTCC TCCACCTCCT 
TTGCCTGGGG GTGTTTGCAT CTCCTCACCC CCTTCTTTAC CTGGAGGTAC TGCTATCTCT 
CCACCCCCTC CTTTGTCTGG GGATGCTACC ATCCCTCCAC CCCCTCCTTT GCCTGAGGGT 
GTTGGCATCC CTTCACCCTC TTCTTTGCCT GGAGGTACTG CCATCCCCCC ACCTCCTCCT 
TTGCCTGGGA GTGCTAGAAT CCCCCCACCA CCACCTCCTT TGCCTGGGAG TGCTGGAATT 
CCCCCCCCAC CTCCTCCCTT GCCTGGAGAA GCAGGAATGC CACCTCCTCC TCCCCCTCTT 
CCTGGTGGTC CTGGAATCCC TCCACCTCCT CCATTTCCCG GAGGCCCTGG CATTCCTCCA 
CCTCCACCCG GAATGGGTAT GCCTCCACCT CCCCCATTTG GATTTGGAGT TCCTGCAGCC 
CCAGTTCTGC CATTTGGATT AACCCCCAAA AAGCTTTATA AGCCAGAGGT GCAGCTCCGG 
AGGCCAAACT GGTCCAAGCT TGTGGCTGAG GACCTCTCCC AGGACTGCTT CTGGACAAAG 
GTGAAGGAGG ACCGCTTTGA GAACAATGAA CTTTTCGCCA AACTTACCCT TACCTTCTCT 
GCCCAGACCA AGACTTCCAA AGCCAAGAAG GATCAAGAAG GTGGAGAAGA AAAGAAATCT 
GTGCAAAAGA AAAAAGTAAA AGAGTTAAAG GTGTTGGATT CAAAGACAGC CCAGAATCTC 
TCAATCTTTT TGGGTTCCTT CCGCATGCCC TATCAAGAGA TTAAGAATGT CATCCTGGAG 
GTGAATGAGG CTGTTCTGAC TGAGTCTATG ATCCAGAACC TCATTAAGCA AATGCCAGAG 
CCAGAGCAGT TAAAAATGCT TTCTGAACTG AAGGATGAAT ATGATGACCT GGCTGAGTCA 
GAGCAGTTTG GCGTGGTGAT GGGCACTGTG CCCCGACTGC GGCCTCGCCT CAATGCCATT 
CTCTTCAAGC TACAATTCAG CGAGCAAGTG GAGAATATCA AGCCAGAGAT TGTGTCTGTC 
ACTGCTGCAT GTGAGGAGTT ACGTAAGAGT GAGAGCTTTT CCAATCTCCT AGAGATTACC 
TTGCTTGTTG GAAATTACAT GAATGCTGGC TCCAGAAATG CTGGTGCTTT TGGCTTCAAT 
ATCAGCTTCC TCTGTAAGCT TCGAGACACC AAGTCCACAG ATCAGAAGAT GACGTTGTTA 
CACTTCTTGG CTGAGTTGTG TGAGAATGAC TATCCCGATG TCCTCAAGTT TCCAGACGAG 
CTTGCCCATG TGGAGAAAGC CAGCCGAGTT TCTGCTGAAA ACTTGCAAAA GAACCTAGAT 
CAGATGAAGA AACAAATTTC TGATGTGGAA CGTGATGTTC AGAATTTCCC AGCTGCCACA 
GATGAAAAAG ACAAGTTTGT TGAAAAAATG ACCAGCTTTG TGAAGGATGC ACAGGAACAG 
TATAACAAGC TGCGGATGAT GCATTCTAAC ATGGAGACCC TCTATAAGGA GCTGGGCGAG 
TACTTCCTCT TTGACCCCAA GAAGTTGTCT GTTGAAGAAT TTTTCATGGA TCTTCACAAT 
TTTCGGAATA TGTTTTTGCA AGCAGTCAAG GAGAACCAGA AGCGGCGGGA GACAGAAGAA 
AAGATGAGGC GAGCAAAACT AGCCAAGGAG AAGGCAGAGA AGGAGCGGCT AGAGAAGCAG 
CAGAAGAGAG AGCAACTCAT AGACATGAAT GCAGAGGGCG ATGAGACAGG TGTGATGGAC 
AGTCTTCTAG AAGCCCTGCA GTCAGGGGCA GCATTCCGAC GGAAGAGAGG GCCCCGTCAA 
GCCAACAGGA AGGCCGGGTG TGCAGTCACA TCTCTGCTAG CTTCGGAGCT GACCAAGGAT 
GATGCCATGG CTGCTGTTCC TGCCAAGGTG TCCAAGAACA GTGAGACATT CCCCACAATC 
CTTGAGGAAG CCAAGGAGTT GGTTGGCCGT GCAAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DIAPH1 ( NM_005219.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_005210.3 Homo sapiens diaphanous related formin 1 (DIAPH1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu26409
Clone ID Related Accession (Same CDS sequence)	NM_005219.4 , NM_005219.5
Accession Version	NM_005219.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3819bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-09
Organism	Homo sapiens(human)
Product	protein diaphanous homolog 1 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA593734.1, AB209482.1, AY363395.1 and AY007129.1. This sequence is a reference standard in the RefSeqGene project. On Nov 22, 2018 this sequence version replaced NM_005219.4. Transcript Variant: This variant (1) encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB209482.1, BC143413.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000389054.8/ ENSP00000373706.4 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGAGCCGC CCGGCGGGAG CCTGGGGCCC GGCCGCGGGA CCCGGGACAA GAAGAAGGGC 
CGGAGCCCAG ATGAGCTGCC CTCGGCGGGC GGCGACGGCG GCAAATCTAA GAAATTTACT 
CTGAAGCGGC TCATGGCAGA TGAGCTGGAG AGATTTACCA GCATGAGAAT TAAGAAGGAG 
AAGGAAAAGC CCAATTCTGC TCATAGAAAT TCTTCTGCAT CATATGGGGA TGATCCCACA 
GCACAGTCAT TGCAAGATGT TTCAGATGAA CAAGTGCTGG TTCTCTTTGA ACAGATGCTG 
CTGGATATGA ACCTGAATGA GGAGAAACAG CAACCTTTGA GGGAGAAGGA CATCATCATC 
AAGAGGGAGA TGGTGTCCCA ATACTTGTAC ACCTCCAAGG CTGGCATGAG CCAGAAGGAG 
AGCTCTAAGT CTGCCATGAT GTATATTCAG GAGTTGAGGT CAGGCTTGCG GGATATGCCT 
CTGCTCAGCT GCCTGGAGTC CCTTCGTGTG TCTCTCAACA ACAACCCTGT CAGTTGGGTG 
CAAACATTTG GTGCTGAAGG CTTGGCCTCC TTATTGGACA TTCTTAAACG ACTTCATGAT 
GAGAAAGAAG AGACTGCTGG GAGTTACGAT AGCCGGAACA AGCATGAGAT CATTCGCTGC 
TTGAAAGCTT TTATGAACAA CAAGTTTGGA ATCAAGACCA TGTTGGAGAC AGAAGAAGGA 
ATCCTACTGC TGGTCAGAGC CATGGATCCT GCTGTTCCCA ACATGATGAT TGATGCAGCT 
AAGCTGCTTT CTGCTCTTTG TATTCTACCG CAGCCAGAGG ACATGAATGA AAGGGTTTTG 
GAGGCAATGA CAGAAAGAGC TGAGATGGAT GAAGTGGAAC GTTTCCAGCC GCTGCTGGAT 
GGATTAAAAA GTGGAACCAC TATTGCACTG AAGGTTGGAT GCCTACAGCT GATCAATGCT 
CTCATCACAC CAGCGGAGGA ACTTGACTTC CGAGTTCACA TCAGAAGTGA ACTGATGCGT 
TTGGGGCTAC ATCAGGTGTT GCAGGACCTT CGAGAGATTG AAAATGAAGA TATGAGAGTG 
CAACTAAATG TGTTTGATGA ACAAGGGGAA GAGGATTCCT ATGACCTGAA GGGACGGCTG 
GATGACATTC GCATGGAGAT GGATGACTTT AATGAAGTCT TTCAGATTCT CTTAAACACA 
GTGAAGGATT CAAAGGCAGA GCCACACTTC CTTTCCATCC TGCAGCACTT ACTCTTGGTC 
CGAAATGACT ATGAGGCCAG ACCTCAGTAC TATAAGTTGA TTGAAGAATG TATTTCCCAG 
ATAGTTCTGC ACAAGAACGG GGCTGATCCT GACTTCAAGT GCCGGCACCT CCAGATTGAG 
ATTGAGGGAT TAATTGATCA AATGATTGAT AAGACAAAGG TGGAGAAATC TGAAGCCAAA 
GCTGCAGAGC TGGAAAAGAA GTTGGACTCA GAGTTAACAG CCCGACATGA GCTACAGGTG 
GAAATGAAAA AGATGGAAAG TGACTTTGAG CAGAAGCTTC AAGATCTTCA GGGAGAAAAA 
GATGCACTGC ATTCTGAAAA GCAGCAAATT GCCACAGAGA AACAGGACCT GGAAGCAGAG 
GTGTCCCAGC TCACAGGAGA GGTTGCCAAG CTGACAAAGG AACTGGAAGA TGCCAAGAAA 
GAAATGGCTT CCCTCTCTGC GGCAGCTATT ACTGTACCTC CTTCTGTTCC TAGTCGTGCT 
CCTGTTCCCC CTGCCCCTCC TTTACCTGGT GACTCTGGCA CTATTATTCC ACCACCACCT 
GCTCCTGGGG ATAGTACCAC TCCTCCTCCT CCTCCTCCTC CTCCTCCTCC TCCACCTCCT 
TTGCCTGGGG GTGTTTGCAT CTCCTCACCC CCTTCTTTAC CTGGAGGTAC TGCTATCTCT 
CCACCCCCTC CTTTGTCTGG GGATGCTACC ATCCCTCCAC CCCCTCCTTT GCCTGAGGGT 
GTTGGCATCC CTTCACCCTC TTCTTTGCCT GGAGGTACTG CCATCCCCCC ACCTCCTCCT 
TTGCCTGGGA GTGCTAGAAT CCCCCCACCA CCACCTCCTT TGCCTGGGAG TGCTGGAATT 
CCCCCCCCAC CTCCTCCCTT GCCTGGAGAA GCAGGAATGC CACCTCCTCC TCCCCCTCTT 
CCTGGTGGTC CTGGAATCCC TCCACCTCCT CCATTTCCCG GAGGCCCTGG CATTCCTCCA 
CCTCCACCCG GAATGGGTAT GCCTCCACCT CCCCCATTTG GATTTGGAGT TCCTGCAGCC 
CCAGTTCTGC CATTTGGATT AACCCCCAAA AAGCTTTATA AGCCAGAGGT GCAGCTCCGG 
AGGCCAAACT GGTCCAAGCT TGTGGCTGAG GACCTCTCCC AGGACTGCTT CTGGACAAAG 
GTGAAGGAGG ACCGCTTTGA GAACAATGAA CTTTTCGCCA AACTTACCCT TACCTTCTCT 
GCCCAGACCA AGACTTCCAA AGCCAAGAAG GATCAAGAAG GTGGAGAAGA AAAGAAATCT 
GTGCAAAAGA AAAAAGTAAA AGAGTTAAAG GTGTTGGATT CAAAGACAGC CCAGAATCTC 
TCAATCTTTT TGGGTTCCTT CCGCATGCCC TATCAAGAGA TTAAGAATGT CATCCTGGAG 
GTGAATGAGG CTGTTCTGAC TGAGTCTATG ATCCAGAACC TCATTAAGCA AATGCCAGAG 
CCAGAGCAGT TAAAAATGCT TTCTGAACTG AAGGATGAAT ATGATGACCT GGCTGAGTCA 
GAGCAGTTTG GCGTGGTGAT GGGCACTGTG CCCCGACTGC GGCCTCGCCT CAATGCCATT 
CTCTTCAAGC TACAATTCAG CGAGCAAGTG GAGAATATCA AGCCAGAGAT TGTGTCTGTC 
ACTGCTGCAT GTGAGGAGTT ACGTAAGAGT GAGAGCTTTT CCAATCTCCT AGAGATTACC 
TTGCTTGTTG GAAATTACAT GAATGCTGGC TCCAGAAATG CTGGTGCTTT TGGCTTCAAT 
ATCAGCTTCC TCTGTAAGCT TCGAGACACC AAGTCCACAG ATCAGAAGAT GACGTTGTTA 
CACTTCTTGG CTGAGTTGTG TGAGAATGAC TATCCCGATG TCCTCAAGTT TCCAGACGAG 
CTTGCCCATG TGGAGAAAGC CAGCCGAGTT TCTGCTGAAA ACTTGCAAAA GAACCTAGAT 
CAGATGAAGA AACAAATTTC TGATGTGGAA CGTGATGTTC AGAATTTCCC AGCTGCCACA 
GATGAAAAAG ACAAGTTTGT TGAAAAAATG ACCAGCTTTG TGAAGGATGC ACAGGAACAG 
TATAACAAGC TGCGGATGAT GCATTCTAAC ATGGAGACCC TCTATAAGGA GCTGGGCGAG 
TACTTCCTCT TTGACCCCAA GAAGTTGTCT GTTGAAGAAT TTTTCATGGA TCTTCACAAT 
TTTCGGAATA TGTTTTTGCA AGCAGTCAAG GAGAACCAGA AGCGGCGGGA GACAGAAGAA 
AAGATGAGGC GAGCAAAACT AGCCAAGGAG AAGGCAGAGA AGGAGCGGCT AGAGAAGCAG 
CAGAAGAGAG AGCAACTCAT AGACATGAAT GCAGAGGGCG ATGAGACAGG TGTGATGGAC 
AGTCTTCTAG AAGCCCTGCA GTCAGGGGCA GCATTCCGAC GGAAGAGAGG GCCCCGTCAA 
GCCAACAGGA AGGCCGGGTG TGCAGTCACA TCTCTGCTAG CTTCGGAGCT GACCAAGGAT 
GATGCCATGG CTGCTGTTCC TGCCAAGGTG TCCAAGAACA GTGAGACATT CCCCACAATC 
CTTGAGGAAG CCAAGGAGTT GGTTGGCCGT GCAAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005210.3
CDS	87..3905
Translation	MEPPGGSLGPGRGTRDKKKGRSPDELPSAGGDGGKSKKFTLKRLMADELERFTSMRIKKEKEKPNSAHRNSSASYGDDPTAQSLQDVSDEQVLVLFEQMLLDMNLNEEKQQPLREKDIIIKREMVSQYLYTSKAGMSQKESSKSAMMYIQELRSGLRDMPLLSCLESLRVSLNNNPVSWVQTFGAEGLASLLDILKRLHDEKEETAGSYDSRNKHEIIRCLKAFMNNKFGIKTMLETEEGILLLVRAMDPAVPNMMIDAAKLLSALCILPQPEDMNERVLEAMTERAEMDEVERFQPLLDGLKSGTTIALKVGCLQLINALITPAEELDFRVHIRSELMRLGLHQVLQDLREIENEDMRVQLNVFDEQGEEDSYDLKGRLDDIRMEMDDFNEVFQILLNTVKDSKAEPHFLSILQHLLLVRNDYEARPQYYKLIEECISQIVLHKNGADPDFKCRHLQIEIEGLIDQMIDKTKVEKSEAKAAELEKKLDSELTARHELQVEMKKMESDFEQKLQDLQGEKDALHSEKQQIATEKQDLEAEVSQLTGEVAKLTKELEDAKKEMASLSAAAITVPPSVPSRAPVPPAPPLPGDSGTIIPPPPAPGDSTTPPPPPPPPPPPPPLPGGVCISSPPSLPGGTAISPPPPLSGDATIPPPPPLPEGVGIPSPSSLPGGTAIPPPPPLPGSARIPPPPPPLPGSAGIPPPPPPLPGEAGMPPPPPPLPGGPGIPPPPPFPGGPGIPPPPPGMGMPPPPPFGFGVPAAPVLPFGLTPKKLYKPEVQLRRPNWSKLVAEDLSQDCFWTKVKEDRFENNELFAKLTLTFSAQTKTSKAKKDQEGGEEKKSVQKKKVKELKVLDSKTAQNLSIFLGSFRMPYQEIKNVILEVNEAVLTESMIQNLIKQMPEPEQLKMLSELKDEYDDLAESEQFGVVMGTVPRLRPRLNAILFKLQFSEQVENIKPEIVSVTAACEELRKSESFSNLLEITLLVGNYMNAGSRNAGAFGFNISFLCKLRDTKSTDQKMTLLHFLAELCENDYPDVLKFPDELAHVEKASRVSAENLQKNLDQMKKQISDVERDVQNFPAATDEKDKFVEKMTSFVKDAQEQYNKLRMMHSNMETLYKELGEYFLFDPKKLSVEEFFMDLHNFRNMFLQAVKENQKRRETEEKMRRAKLAKEKAEKERLEKQQKREQLIDMNAEGDETGVMDSLLEALQSGAAFRRKRGPRQANRKAGCAVTSLLASELTKDDAMAAVPAKVSKNSETFPTILEEAKELVGRAS

Target ORF information:

RefSeq Version	NM_005219.5
Organism	Homo sapiens(human)
Definition	Homo sapiens diaphanous related formin 1 (DIAPH1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005219.5

ORF Insert Sequence:

ATGGAGCCGC CCGGCGGGAG CCTGGGGCCC GGCCGCGGGA CCCGGGACAA GAAGAAGGGC 
CGGAGCCCAG ATGAGCTGCC CTCGGCGGGC GGCGACGGCG GCAAATCTAA GAAATTTACT 
CTGAAGCGGC TCATGGCAGA TGAGCTGGAG AGATTTACCA GCATGAGAAT TAAGAAGGAG 
AAGGAAAAGC CCAATTCTGC TCATAGAAAT TCTTCTGCAT CATATGGGGA TGATCCCACA 
GCACAGTCAT TGCAAGATGT TTCAGATGAA CAAGTGCTGG TTCTCTTTGA ACAGATGCTG 
CTGGATATGA ACCTGAATGA GGAGAAACAG CAACCTTTGA GGGAGAAGGA CATCATCATC 
AAGAGGGAGA TGGTGTCCCA ATACTTGTAC ACCTCCAAGG CTGGCATGAG CCAGAAGGAG 
AGCTCTAAGT CTGCCATGAT GTATATTCAG GAGTTGAGGT CAGGCTTGCG GGATATGCCT 
CTGCTCAGCT GCCTGGAGTC CCTTCGTGTG TCTCTCAACA ACAACCCTGT CAGTTGGGTG 
CAAACATTTG GTGCTGAAGG CTTGGCCTCC TTATTGGACA TTCTTAAACG ACTTCATGAT 
GAGAAAGAAG AGACTGCTGG GAGTTACGAT AGCCGGAACA AGCATGAGAT CATTCGCTGC 
TTGAAAGCTT TTATGAACAA CAAGTTTGGA ATCAAGACCA TGTTGGAGAC AGAAGAAGGA 
ATCCTACTGC TGGTCAGAGC CATGGATCCT GCTGTTCCCA ACATGATGAT TGATGCAGCT 
AAGCTGCTTT CTGCTCTTTG TATTCTACCG CAGCCAGAGG ACATGAATGA AAGGGTTTTG 
GAGGCAATGA CAGAAAGAGC TGAGATGGAT GAAGTGGAAC GTTTCCAGCC GCTGCTGGAT 
GGATTAAAAA GTGGAACCAC TATTGCACTG AAGGTTGGAT GCCTACAGCT GATCAATGCT 
CTCATCACAC CAGCGGAGGA ACTTGACTTC CGAGTTCACA TCAGAAGTGA ACTGATGCGT 
TTGGGGCTAC ATCAGGTGTT GCAGGACCTT CGAGAGATTG AAAATGAAGA TATGAGAGTG 
CAACTAAATG TGTTTGATGA ACAAGGGGAA GAGGATTCCT ATGACCTGAA GGGACGGCTG 
GATGACATTC GCATGGAGAT GGATGACTTT AATGAAGTCT TTCAGATTCT CTTAAACACA 
GTGAAGGATT CAAAGGCAGA GCCACACTTC CTTTCCATCC TGCAGCACTT ACTCTTGGTC 
CGAAATGACT ATGAGGCCAG ACCTCAGTAC TATAAGTTGA TTGAAGAATG TATTTCCCAG 
ATAGTTCTGC ACAAGAACGG GGCTGATCCT GACTTCAAGT GCCGGCACCT CCAGATTGAG 
ATTGAGGGAT TAATTGATCA AATGATTGAT AAGACAAAGG TGGAGAAATC TGAAGCCAAA 
GCTGCAGAGC TGGAAAAGAA GTTGGACTCA GAGTTAACAG CCCGACATGA GCTACAGGTG 
GAAATGAAAA AGATGGAAAG TGACTTTGAG CAGAAGCTTC AAGATCTTCA GGGAGAAAAA 
GATGCACTGC ATTCTGAAAA GCAGCAAATT GCCACAGAGA AACAGGACCT GGAAGCAGAG 
GTGTCCCAGC TCACAGGAGA GGTTGCCAAG CTGACAAAGG AACTGGAAGA TGCCAAGAAA 
GAAATGGCTT CCCTCTCTGC GGCAGCTATT ACTGTACCTC CTTCTGTTCC TAGTCGTGCT 
CCTGTTCCCC CTGCCCCTCC TTTACCTGGT GACTCTGGCA CTATTATTCC ACCACCACCT 
GCTCCTGGGG ATAGTACCAC TCCTCCTCCT CCTCCTCCTC CTCCTCCTCC TCCACCTCCT 
TTGCCTGGGG GTGTTTGCAT CTCCTCACCC CCTTCTTTAC CTGGAGGTAC TGCTATCTCT 
CCACCCCCTC CTTTGTCTGG GGATGCTACC ATCCCTCCAC CCCCTCCTTT GCCTGAGGGT 
GTTGGCATCC CTTCACCCTC TTCTTTGCCT GGAGGTACTG CCATCCCCCC ACCTCCTCCT 
TTGCCTGGGA GTGCTAGAAT CCCCCCACCA CCACCTCCTT TGCCTGGGAG TGCTGGAATT 
CCCCCCCCAC CTCCTCCCTT GCCTGGAGAA GCAGGAATGC CACCTCCTCC TCCCCCTCTT 
CCTGGTGGTC CTGGAATCCC TCCACCTCCT CCATTTCCCG GAGGCCCTGG CATTCCTCCA 
CCTCCACCCG GAATGGGTAT GCCTCCACCT CCCCCATTTG GATTTGGAGT TCCTGCAGCC 
CCAGTTCTGC CATTTGGATT AACCCCCAAA AAGCTTTATA AGCCAGAGGT GCAGCTCCGG 
AGGCCAAACT GGTCCAAGCT TGTGGCTGAG GACCTCTCCC AGGACTGCTT CTGGACAAAG 
GTGAAGGAGG ACCGCTTTGA GAACAATGAA CTTTTCGCCA AACTTACCCT TACCTTCTCT 
GCCCAGACCA AGACTTCCAA AGCCAAGAAG GATCAAGAAG GTGGAGAAGA AAAGAAATCT 
GTGCAAAAGA AAAAAGTAAA AGAGTTAAAG GTGTTGGATT CAAAGACAGC CCAGAATCTC 
TCAATCTTTT TGGGTTCCTT CCGCATGCCC TATCAAGAGA TTAAGAATGT CATCCTGGAG 
GTGAATGAGG CTGTTCTGAC TGAGTCTATG ATCCAGAACC TCATTAAGCA AATGCCAGAG 
CCAGAGCAGT TAAAAATGCT TTCTGAACTG AAGGATGAAT ATGATGACCT GGCTGAGTCA 
GAGCAGTTTG GCGTGGTGAT GGGCACTGTG CCCCGACTGC GGCCTCGCCT CAATGCCATT 
CTCTTCAAGC TACAATTCAG CGAGCAAGTG GAGAATATCA AGCCAGAGAT TGTGTCTGTC 
ACTGCTGCAT GTGAGGAGTT ACGTAAGAGT GAGAGCTTTT CCAATCTCCT AGAGATTACC 
TTGCTTGTTG GAAATTACAT GAATGCTGGC TCCAGAAATG CTGGTGCTTT TGGCTTCAAT 
ATCAGCTTCC TCTGTAAGCT TCGAGACACC AAGTCCACAG ATCAGAAGAT GACGTTGTTA 
CACTTCTTGG CTGAGTTGTG TGAGAATGAC TATCCCGATG TCCTCAAGTT TCCAGACGAG 
CTTGCCCATG TGGAGAAAGC CAGCCGAGTT TCTGCTGAAA ACTTGCAAAA GAACCTAGAT 
CAGATGAAGA AACAAATTTC TGATGTGGAA CGTGATGTTC AGAATTTCCC AGCTGCCACA 
GATGAAAAAG ACAAGTTTGT TGAAAAAATG ACCAGCTTTG TGAAGGATGC ACAGGAACAG 
TATAACAAGC TGCGGATGAT GCATTCTAAC ATGGAGACCC TCTATAAGGA GCTGGGCGAG 
TACTTCCTCT TTGACCCCAA GAAGTTGTCT GTTGAAGAAT TTTTCATGGA TCTTCACAAT 
TTTCGGAATA TGTTTTTGCA AGCAGTCAAG GAGAACCAGA AGCGGCGGGA GACAGAAGAA 
AAGATGAGGC GAGCAAAACT AGCCAAGGAG AAGGCAGAGA AGGAGCGGCT AGAGAAGCAG 
CAGAAGAGAG AGCAACTCAT AGACATGAAT GCAGAGGGCG ATGAGACAGG TGTGATGGAC 
AGTCTTCTAG AAGCCCTGCA GTCAGGGGCA GCATTCCGAC GGAAGAGAGG GCCCCGTCAA 
GCCAACAGGA AGGCCGGGTG TGCAGTCACA TCTCTGCTAG CTTCGGAGCT GACCAAGGAT 
GATGCCATGG CTGCTGTTCC TGCCAAGGTG TCCAAGAACA GTGAGACATT CCCCACAATC 
CTTGAGGAAG CCAAGGAGTT GGTTGGCCGT GCAAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DIAPH1 ( XM_024454384.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024310152.1 Homo sapiens diaphanous related formin 1 (DIAPH1), transcript variant X1, mRNA.

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CloneID	OHu105637
Clone ID Related Accession (Same CDS sequence)	XM_024454384.1
Accession Version	XM_024454384.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3942bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	protein diaphanous homolog 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCCGC CCGGCGGGAG CCTGGGGCCC GGCCGCGGGA CCCGGGACAA GAAGAAGGGC 
CGGAGCCCAG ATGAGCTGCC CTCGGCGGGC GGCGACGGCG GCAAATCTAA GAAATTTACT 
CTGAAGCGGC TCATGGCAGA TGAGCTGGAG AGATTTACCA GCATGAGAAT TAAGAAGGAG 
AAGGAAAAGC CCAATTCTGC TCATAGAAAT TCTTCTGCAT CATATGGGGA TGATCCCACA 
GCACAGTCAT TGCAAGATGT TTCAGATGAA CAAGTGCTGG TTCTCTTTGA ACAGATGCTG 
CTGGATATGA ACCTGAATGA GGAGAAACAG CAACCTTTGA GGGAGAAGGA CATCATCATC 
AAGAGGGAGA TGGTGTCCCA ATACTTGTAC ACCTCCAAGG CTGGCATGAG CCAGAAGGAG 
AGCTCTAAGT CTGCCATGAT GTATATTCAG GAGTTGAGGT CAGGCTTGCG GGATATGCCT 
CTGCTCAGCT GCCTGGAGTC CCTTCGTGTG TCTCTCAACA ACAACCCTGT CAGTTGGGTG 
CAAACATTTG GTGCTGAAGG CTTGGCCTCC TTATTGGACA TTCTTAAACG ACTTCATGAT 
GAGAAAGAAG AGACTGCTGG GAGTTACGAT AGCCGGAACA AGCATGAGAT CATTCGCTGC 
TTGAAAGCTT TTATGAACAA CAAGTTTGGA ATCAAGACCA TGTTGGAGAC AGAAGAAGGA 
ATCCTACTGC TGGTCAGAGC CATGGATCCT GCTGTTCCCA ACATGATGAT TGATGCAGCT 
AAGCTGCTTT CTGCTCTTTG TATTCTACCG CAGCCAGAGG ACATGAATGA AAGGGTTTTG 
GAGGCAATGA CAGAAAGAGC TGAGATGGAT GAAGTGGAAC GTTTCCAGCC GCTGCTGGAT 
GGATTAAAAA GTGGAACCAC TATTGCACTG AAGGTTGGAT GCCTACAGCT GATCAATGCT 
CTCATCACAC CAGCGGAGGA ACTTGACTTC CGAGTTCACA TCAGAAGTGA ACTGATGCGT 
TTGGGGCTAC ATCAGGTGTT GCAGGACCTT CGAGAGATTG AAAATGAAGA TATGAGAGTG 
CAACTAAATG TGTTTGATGA ACAAGGGGAA GAGGATTCCT ATGACCTGAA GGGACGGCTG 
GATGACATTC GCATGGAGAT GGATGACTTT AATGAAGTCT TTCAGATTCT CTTAAACACA 
GTGAAGGATT CAAAGGCAGA GCCACACTTC CTTTCCATCC TGCAGCACTT ACTCTTGGTC 
CGAAATGACT ATGAGGCCAG ACCTCAGTAC TATAAGTTGA TTGAAGAATG TATTTCCCAG 
ATAGTTCTGC ACAAGAACGG GGCTGATCCT GACTTCAAGT GCCGGCACCT CCAGATTGAG 
ATTGAGGGAT TAATTGATCA AATGATTGAT AAGACAAAGG TGGAGAAATC TGAAGCCAAA 
GCTGCAGAGC TGGAAAAGAA GTTGGACTCA GAGTTAACAG CCCGACATGA GCTACAGGTG 
GAAATGAAAA AGATGGAAAG TGACTTTGAG CAGAAGCTTC AAGATCTTCA GGGAGAAAAA 
GATGCACTGC ATTCTGAAAA GCAGCAAATT GCCACAGAGA AACAGGACCT GGAAGCAGAG 
GTGTCCCAGC TCACAGGAGA GGTTGCCAAG CTGACAAAGG AACTGGAAGA TGCCAAGAAA 
GAAATGGCTT CCCTCTCTGC GGCAGCTATT ACTGTACCTC CTTCTGTTCC TAGTCGTGCT 
CCTGTTCCCC CTGCCCCTCC TTTACCTGGT GACTCTGGCA CTATTATTCC ACCACCACCT 
GCTCCTGGGG ATAGTACCAC TCCTCCTCCT CCTCCTCCTC CTCCTCCTCC TCCACCTCCT 
TTGCCTGGGG GTGTTTGCAT CTCCTCACCC CCTTCTTTAC CTGGAGGTAC TGCTATCTCT 
CCACCCCCTC CTTTGTCTGG GGATGCTACC ATCCCTCCAC CCCCTCCTTT GCCTGAGGGT 
GTTGGCATCC CTTCACCCTC TTCTTTGCCT GGAGGTACTG CCATCCCCCC ACCTCCTCCT 
TTGCCTGGGA GTGCTAGAAT CCCCCCACCA CCACCTCCTT TGCCTGGGAG TGCTGGAATT 
CCCCCCCCAC CTCCTCCCTT GCCTGGAGAA GCAGGAATGC CACCTCCTCC TCCCCCTCTT 
CCTGGTGGTC CTGGAATCCC TCCACCTCCT CCATTTCCCG GAGGCCCTGG CATTCCTCCA 
CCTCCACCCG GAATGGGTAT GCCTCCACCT CCCCCATTTG GATTTGGAGT TCCTGCAGCC 
CCAGTTCTGC CATTTGGATT AACCCCCAAA AAGCTTTATA AGCCAGAGGT GCAGCTCCGG 
AGGCCAAACT GGTCCAAGCT TGTGGCTGAG GACCTCTCCC AGGACTGCTT CTGGACAAAG 
GTGAAGGAGG ACCGCTTTGA GAACAATGAA CTTTTCGCCA AACTTACCCT TACCTTCTCT 
GCCCAGACCA AGACTTCCAA AGCCAAGAAG GATCAAGAAG GTGGAGAAGA AAAGAAATCT 
GTGCAAAAGA AAAAAGTAAA AGAGTTAAAG GTGTTGGATT CAAAGACAGC CCAGAATCTC 
TCAATCTTTT TGGGTTCCTT CCGCATGCCC TATCAAGAGA TTAAGAATGT CATCCTGGAG 
GTGAATGAGG CTGTTCTGAC TGAGTCTATG ATCCAGAACC TCATTAAGCA AATGCCAGAG 
CCAGAGCAGT TAAAAATGCT TTCTGAACTG AAGGATGAAT ATGATGACCT GGCTGAGTCA 
GAGCAGTTTG GCGTGGTGAT GGGCACTGTG CCCCGACTGC GGCCTCGCCT CAATGCCATT 
CTCTTCAAGC TACAATTCAG CGAGCAAGTG GAGAATATCA AGCCAGAGAT TGTGTCTGTC 
ACTGCTGCAT GTGAGGAGTT ACGTAAGAGT GAGAGCTTTT CCAATCTCCT AGAGATTACC 
TTGCTTGTTG GAAATTACAT GAATGCTGGC TCCAGAAATG CTGGTGCTTT TGGCTTCAAT 
ATCAGCTTCC TCTGTAAGCT TCGAGACACC AAGTCCACAG ATCAGAAGAT GACGTTGTTA 
CACTTCTTGG CTGAGTTGTG TGAGAATGAC TATCCCGATG TCCTCAAGTT TCCAGACGAG 
CTTGCCCATG TGGAGAAAGC CAGCCGAGTT TCTGCTGAAA ACTTGCAAAA GAACCTAGAT 
CAGATGAAGA AACAAATTTC TGATGTGGAA CGTGATGTTC AGAATTTCCC AGCTGCCACA 
GATGAAAAAG ACAAGTTTGT TGAAAAAATG ACCAGCTTTG TGAAGGATGC ACAGGAACAG 
TATAACAAGC TGCGGATGAT GCATTCTAAC ATGGAGACCC TCTATAAGGA GCTGGGCGAG 
TACTTCCTCT TTGACCCCAA GAAGTTGTCT GTTGAAGAAT TTTTCATGGA TCTTCACAAT 
TTTCGGAATA TGTTTTTGGT GAGCAAGGGA TACTTGCTGA CTGAATCTTT TGTGGGCATT 
TCTCACTTTC CCCTGGGTTC CTGTTGGCTG GTAGTAGGTA TACAATGCCC ATGTGTTCCT 
TCCTCCTCTT CCCTGCCCCA GCAAGCAGTC AAGGAGAACC AGAAGCGGCG GGAGACAGAA 
GAAAAGATGA GGCGAGCAAA ACTAGCCAAG GAGAAGGCAG AGAAGGAGCG GCTAGAGAAG 
CAGCAGAAGA GAGAGCAACT CATAGACATG AATGCAGAGG GCGATGAGAC AGGTGTGATG 
GACAGTCTTC TAGAAGCCCT GCAGTCAGGG GCAGCATTCC GACGGAAGAG AGGGCCCCGT 
CAAGCCAACA GGAAGGCCGG GTGTGCAGTC ACATCTCTGC TAGCTTCGGA GCTGACCAAG 
GATGATGCCA TGGCTGCTGT TCCTGCCAAG GTGTCCAAGA ACAGTGAGAC ATTCCCCACA 
ATCCTTGAGG AAGCCAAGGA GTTGGTTGGC CGTGCAAGCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024310152.1
CDS	142..4083
Translation	MEPPGGSLGPGRGTRDKKKGRSPDELPSAGGDGGKSKKFTLKRLMADELERFTSMRIKKEKEKPNSAHRNSSASYGDDPTAQSLQDVSDEQVLVLFEQMLLDMNLNEEKQQPLREKDIIIKREMVSQYLYTSKAGMSQKESSKSAMMYIQELRSGLRDMPLLSCLESLRVSLNNNPVSWVQTFGAEGLASLLDILKRLHDEKEETAGSYDSRNKHEIIRCLKAFMNNKFGIKTMLETEEGILLLVRAMDPAVPNMMIDAAKLLSALCILPQPEDMNERVLEAMTERAEMDEVERFQPLLDGLKSGTTIALKVGCLQLINALITPAEELDFRVHIRSELMRLGLHQVLQDLREIENEDMRVQLNVFDEQGEEDSYDLKGRLDDIRMEMDDFNEVFQILLNTVKDSKAEPHFLSILQHLLLVRNDYEARPQYYKLIEECISQIVLHKNGADPDFKCRHLQIEIEGLIDQMIDKTKVEKSEAKAAELEKKLDSELTARHELQVEMKKMESDFEQKLQDLQGEKDALHSEKQQIATEKQDLEAEVSQLTGEVAKLTKELEDAKKEMASLSAAAITVPPSVPSRAPVPPAPPLPGDSGTIIPPPPAPGDSTTPPPPPPPPPPPPPLPGGVCISSPPSLPGGTAISPPPPLSGDATIPPPPPLPEGVGIPSPSSLPGGTAIPPPPPLPGSARIPPPPPPLPGSAGIPPPPPPLPGEAGMPPPPPPLPGGPGIPPPPPFPGGPGIPPPPPGMGMPPPPPFGFGVPAAPVLPFGLTPKKLYKPEVQLRRPNWSKLVAEDLSQDCFWTKVKEDRFENNELFAKLTLTFSAQTKTSKAKKDQEGGEEKKSVQKKKVKELKVLDSKTAQNLSIFLGSFRMPYQEIKNVILEVNEAVLTESMIQNLIKQMPEPEQLKMLSELKDEYDDLAESEQFGVVMGTVPRLRPRLNAILFKLQFSEQVENIKPEIVSVTAACEELRKSESFSNLLEITLLVGNYMNAGSRNAGAFGFNISFLCKLRDTKSTDQKMTLLHFLAELCENDYPDVLKFPDELAHVEKASRVSAENLQKNLDQMKKQISDVERDVQNFPAATDEKDKFVEKMTSFVKDAQEQYNKLRMMHSNMETLYKELGEYFLFDPKKLSVEEFFMDLHNFRNMFLVSKGYLLTESFVGISHFPLGSCWLVVGIQCPCVPSSSSLPQQAVKENQKRRETEEKMRRAKLAKEKAEKERLEKQQKREQLIDMNAEGDETGVMDSLLEALQSGAAFRRKRGPRQANRKAGCAVTSLLASELTKDDAMAAVPAKVSKNSETFPTILEEAKELVGRAS

Target ORF information:

RefSeq Version	XM_024454384.1
Organism	Homo sapiens(human)
Definition	Homo sapiens diaphanous related formin 1 (DIAPH1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024454384.1

ORF Insert Sequence:

ATGGAGCCGC CCGGCGGGAG CCTGGGGCCC GGCCGCGGGA CCCGGGACAA GAAGAAGGGC 
CGGAGCCCAG ATGAGCTGCC CTCGGCGGGC GGCGACGGCG GCAAATCTAA GAAATTTACT 
CTGAAGCGGC TCATGGCAGA TGAGCTGGAG AGATTTACCA GCATGAGAAT TAAGAAGGAG 
AAGGAAAAGC CCAATTCTGC TCATAGAAAT TCTTCTGCAT CATATGGGGA TGATCCCACA 
GCACAGTCAT TGCAAGATGT TTCAGATGAA CAAGTGCTGG TTCTCTTTGA ACAGATGCTG 
CTGGATATGA ACCTGAATGA GGAGAAACAG CAACCTTTGA GGGAGAAGGA CATCATCATC 
AAGAGGGAGA TGGTGTCCCA ATACTTGTAC ACCTCCAAGG CTGGCATGAG CCAGAAGGAG 
AGCTCTAAGT CTGCCATGAT GTATATTCAG GAGTTGAGGT CAGGCTTGCG GGATATGCCT 
CTGCTCAGCT GCCTGGAGTC CCTTCGTGTG TCTCTCAACA ACAACCCTGT CAGTTGGGTG 
CAAACATTTG GTGCTGAAGG CTTGGCCTCC TTATTGGACA TTCTTAAACG ACTTCATGAT 
GAGAAAGAAG AGACTGCTGG GAGTTACGAT AGCCGGAACA AGCATGAGAT CATTCGCTGC 
TTGAAAGCTT TTATGAACAA CAAGTTTGGA ATCAAGACCA TGTTGGAGAC AGAAGAAGGA 
ATCCTACTGC TGGTCAGAGC CATGGATCCT GCTGTTCCCA ACATGATGAT TGATGCAGCT 
AAGCTGCTTT CTGCTCTTTG TATTCTACCG CAGCCAGAGG ACATGAATGA AAGGGTTTTG 
GAGGCAATGA CAGAAAGAGC TGAGATGGAT GAAGTGGAAC GTTTCCAGCC GCTGCTGGAT 
GGATTAAAAA GTGGAACCAC TATTGCACTG AAGGTTGGAT GCCTACAGCT GATCAATGCT 
CTCATCACAC CAGCGGAGGA ACTTGACTTC CGAGTTCACA TCAGAAGTGA ACTGATGCGT 
TTGGGGCTAC ATCAGGTGTT GCAGGACCTT CGAGAGATTG AAAATGAAGA TATGAGAGTG 
CAACTAAATG TGTTTGATGA ACAAGGGGAA GAGGATTCCT ATGACCTGAA GGGACGGCTG 
GATGACATTC GCATGGAGAT GGATGACTTT AATGAAGTCT TTCAGATTCT CTTAAACACA 
GTGAAGGATT CAAAGGCAGA GCCACACTTC CTTTCCATCC TGCAGCACTT ACTCTTGGTC 
CGAAATGACT ATGAGGCCAG ACCTCAGTAC TATAAGTTGA TTGAAGAATG TATTTCCCAG 
ATAGTTCTGC ACAAGAACGG GGCTGATCCT GACTTCAAGT GCCGGCACCT CCAGATTGAG 
ATTGAGGGAT TAATTGATCA AATGATTGAT AAGACAAAGG TGGAGAAATC TGAAGCCAAA 
GCTGCAGAGC TGGAAAAGAA GTTGGACTCA GAGTTAACAG CCCGACATGA GCTACAGGTG 
GAAATGAAAA AGATGGAAAG TGACTTTGAG CAGAAGCTTC AAGATCTTCA GGGAGAAAAA 
GATGCACTGC ATTCTGAAAA GCAGCAAATT GCCACAGAGA AACAGGACCT GGAAGCAGAG 
GTGTCCCAGC TCACAGGAGA GGTTGCCAAG CTGACAAAGG AACTGGAAGA TGCCAAGAAA 
GAAATGGCTT CCCTCTCTGC GGCAGCTATT ACTGTACCTC CTTCTGTTCC TAGTCGTGCT 
CCTGTTCCCC CTGCCCCTCC TTTACCTGGT GACTCTGGCA CTATTATTCC ACCACCACCT 
GCTCCTGGGG ATAGTACCAC TCCTCCTCCT CCTCCTCCTC CTCCTCCTCC TCCACCTCCT 
TTGCCTGGGG GTGTTTGCAT CTCCTCACCC CCTTCTTTAC CTGGAGGTAC TGCTATCTCT 
CCACCCCCTC CTTTGTCTGG GGATGCTACC ATCCCTCCAC CCCCTCCTTT GCCTGAGGGT 
GTTGGCATCC CTTCACCCTC TTCTTTGCCT GGAGGTACTG CCATCCCCCC ACCTCCTCCT 
TTGCCTGGGA GTGCTAGAAT CCCCCCACCA CCACCTCCTT TGCCTGGGAG TGCTGGAATT 
CCCCCCCCAC CTCCTCCCTT GCCTGGAGAA GCAGGAATGC CACCTCCTCC TCCCCCTCTT 
CCTGGTGGTC CTGGAATCCC TCCACCTCCT CCATTTCCCG GAGGCCCTGG CATTCCTCCA 
CCTCCACCCG GAATGGGTAT GCCTCCACCT CCCCCATTTG GATTTGGAGT TCCTGCAGCC 
CCAGTTCTGC CATTTGGATT AACCCCCAAA AAGCTTTATA AGCCAGAGGT GCAGCTCCGG 
AGGCCAAACT GGTCCAAGCT TGTGGCTGAG GACCTCTCCC AGGACTGCTT CTGGACAAAG 
GTGAAGGAGG ACCGCTTTGA GAACAATGAA CTTTTCGCCA AACTTACCCT TACCTTCTCT 
GCCCAGACCA AGACTTCCAA AGCCAAGAAG GATCAAGAAG GTGGAGAAGA AAAGAAATCT 
GTGCAAAAGA AAAAAGTAAA AGAGTTAAAG GTGTTGGATT CAAAGACAGC CCAGAATCTC 
TCAATCTTTT TGGGTTCCTT CCGCATGCCC TATCAAGAGA TTAAGAATGT CATCCTGGAG 
GTGAATGAGG CTGTTCTGAC TGAGTCTATG ATCCAGAACC TCATTAAGCA AATGCCAGAG 
CCAGAGCAGT TAAAAATGCT TTCTGAACTG AAGGATGAAT ATGATGACCT GGCTGAGTCA 
GAGCAGTTTG GCGTGGTGAT GGGCACTGTG CCCCGACTGC GGCCTCGCCT CAATGCCATT 
CTCTTCAAGC TACAATTCAG CGAGCAAGTG GAGAATATCA AGCCAGAGAT TGTGTCTGTC 
ACTGCTGCAT GTGAGGAGTT ACGTAAGAGT GAGAGCTTTT CCAATCTCCT AGAGATTACC 
TTGCTTGTTG GAAATTACAT GAATGCTGGC TCCAGAAATG CTGGTGCTTT TGGCTTCAAT 
ATCAGCTTCC TCTGTAAGCT TCGAGACACC AAGTCCACAG ATCAGAAGAT GACGTTGTTA 
CACTTCTTGG CTGAGTTGTG TGAGAATGAC TATCCCGATG TCCTCAAGTT TCCAGACGAG 
CTTGCCCATG TGGAGAAAGC CAGCCGAGTT TCTGCTGAAA ACTTGCAAAA GAACCTAGAT 
CAGATGAAGA AACAAATTTC TGATGTGGAA CGTGATGTTC AGAATTTCCC AGCTGCCACA 
GATGAAAAAG ACAAGTTTGT TGAAAAAATG ACCAGCTTTG TGAAGGATGC ACAGGAACAG 
TATAACAAGC TGCGGATGAT GCATTCTAAC ATGGAGACCC TCTATAAGGA GCTGGGCGAG 
TACTTCCTCT TTGACCCCAA GAAGTTGTCT GTTGAAGAAT TTTTCATGGA TCTTCACAAT 
TTTCGGAATA TGTTTTTGGT GAGCAAGGGA TACTTGCTGA CTGAATCTTT TGTGGGCATT 
TCTCACTTTC CCCTGGGTTC CTGTTGGCTG GTAGTAGGTA TACAATGCCC ATGTGTTCCT 
TCCTCCTCTT CCCTGCCCCA GCAAGCAGTC AAGGAGAACC AGAAGCGGCG GGAGACAGAA 
GAAAAGATGA GGCGAGCAAA ACTAGCCAAG GAGAAGGCAG AGAAGGAGCG GCTAGAGAAG 
CAGCAGAAGA GAGAGCAACT CATAGACATG AATGCAGAGG GCGATGAGAC AGGTGTGATG 
GACAGTCTTC TAGAAGCCCT GCAGTCAGGG GCAGCATTCC GACGGAAGAG AGGGCCCCGT 
CAAGCCAACA GGAAGGCCGG GTGTGCAGTC ACATCTCTGC TAGCTTCGGA GCTGACCAAG 
GATGATGCCA TGGCTGCTGT TCCTGCCAAG GTGTCCAAGA ACAGTGAGAC ATTCCCCACA 
ATCCTTGAGG AAGCCAAGGA GTTGGTTGGC CGTGCAAGCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DIAPH1 ( XM_024454385.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024310153.1 Homo sapiens diaphanous related formin 1 (DIAPH1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu105638
Clone ID Related Accession (Same CDS sequence)	XM_024454385.1
Accession Version	XM_024454385.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3915bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	protein diaphanous homolog 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCCGC CCGGCGGGAG CCTGGGGCCC GGCCGCGGGA CCCGGGACAA GAAGAAGGGC 
CGGAGCCCAG ATGAGCTGCC CTCGGCGGGC GGCGACGGCG GCAAATCTAA GAAATTTCTG 
GAGAGATTTA CCAGCATGAG AATTAAGAAG GAGAAGGAAA AGCCCAATTC TGCTCATAGA 
AATTCTTCTG CATCATATGG GGATGATCCC ACAGCACAGT CATTGCAAGA TGTTTCAGAT 
GAACAAGTGC TGGTTCTCTT TGAACAGATG CTGCTGGATA TGAACCTGAA TGAGGAGAAA 
CAGCAACCTT TGAGGGAGAA GGACATCATC ATCAAGAGGG AGATGGTGTC CCAATACTTG 
TACACCTCCA AGGCTGGCAT GAGCCAGAAG GAGAGCTCTA AGTCTGCCAT GATGTATATT 
CAGGAGTTGA GGTCAGGCTT GCGGGATATG CCTCTGCTCA GCTGCCTGGA GTCCCTTCGT 
GTGTCTCTCA ACAACAACCC TGTCAGTTGG GTGCAAACAT TTGGTGCTGA AGGCTTGGCC 
TCCTTATTGG ACATTCTTAA ACGACTTCAT GATGAGAAAG AAGAGACTGC TGGGAGTTAC 
GATAGCCGGA ACAAGCATGA GATCATTCGC TGCTTGAAAG CTTTTATGAA CAACAAGTTT 
GGAATCAAGA CCATGTTGGA GACAGAAGAA GGAATCCTAC TGCTGGTCAG AGCCATGGAT 
CCTGCTGTTC CCAACATGAT GATTGATGCA GCTAAGCTGC TTTCTGCTCT TTGTATTCTA 
CCGCAGCCAG AGGACATGAA TGAAAGGGTT TTGGAGGCAA TGACAGAAAG AGCTGAGATG 
GATGAAGTGG AACGTTTCCA GCCGCTGCTG GATGGATTAA AAAGTGGAAC CACTATTGCA 
CTGAAGGTTG GATGCCTACA GCTGATCAAT GCTCTCATCA CACCAGCGGA GGAACTTGAC 
TTCCGAGTTC ACATCAGAAG TGAACTGATG CGTTTGGGGC TACATCAGGT GTTGCAGGAC 
CTTCGAGAGA TTGAAAATGA AGATATGAGA GTGCAACTAA ATGTGTTTGA TGAACAAGGG 
GAAGAGGATT CCTATGACCT GAAGGGACGG CTGGATGACA TTCGCATGGA GATGGATGAC 
TTTAATGAAG TCTTTCAGAT TCTCTTAAAC ACAGTGAAGG ATTCAAAGGC AGAGCCACAC 
TTCCTTTCCA TCCTGCAGCA CTTACTCTTG GTCCGAAATG ACTATGAGGC CAGACCTCAG 
TACTATAAGT TGATTGAAGA ATGTATTTCC CAGATAGTTC TGCACAAGAA CGGGGCTGAT 
CCTGACTTCA AGTGCCGGCA CCTCCAGATT GAGATTGAGG GATTAATTGA TCAAATGATT 
GATAAGACAA AGGTGGAGAA ATCTGAAGCC AAAGCTGCAG AGCTGGAAAA GAAGTTGGAC 
TCAGAGTTAA CAGCCCGACA TGAGCTACAG GTGGAAATGA AAAAGATGGA AAGTGACTTT 
GAGCAGAAGC TTCAAGATCT TCAGGGAGAA AAAGATGCAC TGCATTCTGA AAAGCAGCAA 
ATTGCCACAG AGAAACAGGA CCTGGAAGCA GAGGTGTCCC AGCTCACAGG AGAGGTTGCC 
AAGCTGACAA AGGAACTGGA AGATGCCAAG AAAGAAATGG CTTCCCTCTC TGCGGCAGCT 
ATTACTGTAC CTCCTTCTGT TCCTAGTCGT GCTCCTGTTC CCCCTGCCCC TCCTTTACCT 
GGTGACTCTG GCACTATTAT TCCACCACCA CCTGCTCCTG GGGATAGTAC CACTCCTCCT 
CCTCCTCCTC CTCCTCCTCC TCCTCCACCT CCTTTGCCTG GGGGTGTTTG CATCTCCTCA 
CCCCCTTCTT TACCTGGAGG TACTGCTATC TCTCCACCCC CTCCTTTGTC TGGGGATGCT 
ACCATCCCTC CACCCCCTCC TTTGCCTGAG GGTGTTGGCA TCCCTTCACC CTCTTCTTTG 
CCTGGAGGTA CTGCCATCCC CCCACCTCCT CCTTTGCCTG GGAGTGCTAG AATCCCCCCA 
CCACCACCTC CTTTGCCTGG GAGTGCTGGA ATTCCCCCCC CACCTCCTCC CTTGCCTGGA 
GAAGCAGGAA TGCCACCTCC TCCTCCCCCT CTTCCTGGTG GTCCTGGAAT CCCTCCACCT 
CCTCCATTTC CCGGAGGCCC TGGCATTCCT CCACCTCCAC CCGGAATGGG TATGCCTCCA 
CCTCCCCCAT TTGGATTTGG AGTTCCTGCA GCCCCAGTTC TGCCATTTGG ATTAACCCCC 
AAAAAGCTTT ATAAGCCAGA GGTGCAGCTC CGGAGGCCAA ACTGGTCCAA GCTTGTGGCT 
GAGGACCTCT CCCAGGACTG CTTCTGGACA AAGGTGAAGG AGGACCGCTT TGAGAACAAT 
GAACTTTTCG CCAAACTTAC CCTTACCTTC TCTGCCCAGA CCAAGACTTC CAAAGCCAAG 
AAGGATCAAG AAGGTGGAGA AGAAAAGAAA TCTGTGCAAA AGAAAAAAGT AAAAGAGTTA 
AAGGTGTTGG ATTCAAAGAC AGCCCAGAAT CTCTCAATCT TTTTGGGTTC CTTCCGCATG 
CCCTATCAAG AGATTAAGAA TGTCATCCTG GAGGTGAATG AGGCTGTTCT GACTGAGTCT 
ATGATCCAGA ACCTCATTAA GCAAATGCCA GAGCCAGAGC AGTTAAAAAT GCTTTCTGAA 
CTGAAGGATG AATATGATGA CCTGGCTGAG TCAGAGCAGT TTGGCGTGGT GATGGGCACT 
GTGCCCCGAC TGCGGCCTCG CCTCAATGCC ATTCTCTTCA AGCTACAATT CAGCGAGCAA 
GTGGAGAATA TCAAGCCAGA GATTGTGTCT GTCACTGCTG CATGTGAGGA GTTACGTAAG 
AGTGAGAGCT TTTCCAATCT CCTAGAGATT ACCTTGCTTG TTGGAAATTA CATGAATGCT 
GGCTCCAGAA ATGCTGGTGC TTTTGGCTTC AATATCAGCT TCCTCTGTAA GCTTCGAGAC 
ACCAAGTCCA CAGATCAGAA GATGACGTTG TTACACTTCT TGGCTGAGTT GTGTGAGAAT 
GACTATCCCG ATGTCCTCAA GTTTCCAGAC GAGCTTGCCC ATGTGGAGAA AGCCAGCCGA 
GTTTCTGCTG AAAACTTGCA AAAGAACCTA GATCAGATGA AGAAACAAAT TTCTGATGTG 
GAACGTGATG TTCAGAATTT CCCAGCTGCC ACAGATGAAA AAGACAAGTT TGTTGAAAAA 
ATGACCAGCT TTGTGAAGGA TGCACAGGAA CAGTATAACA AGCTGCGGAT GATGCATTCT 
AACATGGAGA CCCTCTATAA GGAGCTGGGC GAGTACTTCC TCTTTGACCC CAAGAAGTTG 
TCTGTTGAAG AATTTTTCAT GGATCTTCAC AATTTTCGGA ATATGTTTTT GGTGAGCAAG 
GGATACTTGC TGACTGAATC TTTTGTGGGC ATTTCTCACT TTCCCCTGGG TTCCTGTTGG 
CTGGTAGTAG GTATACAATG CCCATGTGTT CCTTCCTCCT CTTCCCTGCC CCAGCAAGCA 
GTCAAGGAGA ACCAGAAGCG GCGGGAGACA GAAGAAAAGA TGAGGCGAGC AAAACTAGCC 
AAGGAGAAGG CAGAGAAGGA GCGGCTAGAG AAGCAGCAGA AGAGAGAGCA ACTCATAGAC 
ATGAATGCAG AGGGCGATGA GACAGGTGTG ATGGACAGTC TTCTAGAAGC CCTGCAGTCA 
GGGGCAGCAT TCCGACGGAA GAGAGGGCCC CGTCAAGCCA ACAGGAAGGC CGGGTGTGCA 
GTCACATCTC TGCTAGCTTC GGAGCTGACC AAGGATGATG CCATGGCTGC TGTTCCTGCC 
AAGGTGTCCA AGAACAGTGA GACATTCCCC ACAATCCTTG AGGAAGCCAA GGAGTTGGTT 
GGCCGTGCAA GCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024310153.1
CDS	93..4007
Translation	MEPPGGSLGPGRGTRDKKKGRSPDELPSAGGDGGKSKKFLERFTSMRIKKEKEKPNSAHRNSSASYGDDPTAQSLQDVSDEQVLVLFEQMLLDMNLNEEKQQPLREKDIIIKREMVSQYLYTSKAGMSQKESSKSAMMYIQELRSGLRDMPLLSCLESLRVSLNNNPVSWVQTFGAEGLASLLDILKRLHDEKEETAGSYDSRNKHEIIRCLKAFMNNKFGIKTMLETEEGILLLVRAMDPAVPNMMIDAAKLLSALCILPQPEDMNERVLEAMTERAEMDEVERFQPLLDGLKSGTTIALKVGCLQLINALITPAEELDFRVHIRSELMRLGLHQVLQDLREIENEDMRVQLNVFDEQGEEDSYDLKGRLDDIRMEMDDFNEVFQILLNTVKDSKAEPHFLSILQHLLLVRNDYEARPQYYKLIEECISQIVLHKNGADPDFKCRHLQIEIEGLIDQMIDKTKVEKSEAKAAELEKKLDSELTARHELQVEMKKMESDFEQKLQDLQGEKDALHSEKQQIATEKQDLEAEVSQLTGEVAKLTKELEDAKKEMASLSAAAITVPPSVPSRAPVPPAPPLPGDSGTIIPPPPAPGDSTTPPPPPPPPPPPPPLPGGVCISSPPSLPGGTAISPPPPLSGDATIPPPPPLPEGVGIPSPSSLPGGTAIPPPPPLPGSARIPPPPPPLPGSAGIPPPPPPLPGEAGMPPPPPPLPGGPGIPPPPPFPGGPGIPPPPPGMGMPPPPPFGFGVPAAPVLPFGLTPKKLYKPEVQLRRPNWSKLVAEDLSQDCFWTKVKEDRFENNELFAKLTLTFSAQTKTSKAKKDQEGGEEKKSVQKKKVKELKVLDSKTAQNLSIFLGSFRMPYQEIKNVILEVNEAVLTESMIQNLIKQMPEPEQLKMLSELKDEYDDLAESEQFGVVMGTVPRLRPRLNAILFKLQFSEQVENIKPEIVSVTAACEELRKSESFSNLLEITLLVGNYMNAGSRNAGAFGFNISFLCKLRDTKSTDQKMTLLHFLAELCENDYPDVLKFPDELAHVEKASRVSAENLQKNLDQMKKQISDVERDVQNFPAATDEKDKFVEKMTSFVKDAQEQYNKLRMMHSNMETLYKELGEYFLFDPKKLSVEEFFMDLHNFRNMFLVSKGYLLTESFVGISHFPLGSCWLVVGIQCPCVPSSSSLPQQAVKENQKRRETEEKMRRAKLAKEKAEKERLEKQQKREQLIDMNAEGDETGVMDSLLEALQSGAAFRRKRGPRQANRKAGCAVTSLLASELTKDDAMAAVPAKVSKNSETFPTILEEAKELVGRAS

Target ORF information:

RefSeq Version	XM_024454385.1
Organism	Homo sapiens(human)
Definition	Homo sapiens diaphanous related formin 1 (DIAPH1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024454385.1

ORF Insert Sequence:

ATGGAGCCGC CCGGCGGGAG CCTGGGGCCC GGCCGCGGGA CCCGGGACAA GAAGAAGGGC 
CGGAGCCCAG ATGAGCTGCC CTCGGCGGGC GGCGACGGCG GCAAATCTAA GAAATTTCTG 
GAGAGATTTA CCAGCATGAG AATTAAGAAG GAGAAGGAAA AGCCCAATTC TGCTCATAGA 
AATTCTTCTG CATCATATGG GGATGATCCC ACAGCACAGT CATTGCAAGA TGTTTCAGAT 
GAACAAGTGC TGGTTCTCTT TGAACAGATG CTGCTGGATA TGAACCTGAA TGAGGAGAAA 
CAGCAACCTT TGAGGGAGAA GGACATCATC ATCAAGAGGG AGATGGTGTC CCAATACTTG 
TACACCTCCA AGGCTGGCAT GAGCCAGAAG GAGAGCTCTA AGTCTGCCAT GATGTATATT 
CAGGAGTTGA GGTCAGGCTT GCGGGATATG CCTCTGCTCA GCTGCCTGGA GTCCCTTCGT 
GTGTCTCTCA ACAACAACCC TGTCAGTTGG GTGCAAACAT TTGGTGCTGA AGGCTTGGCC 
TCCTTATTGG ACATTCTTAA ACGACTTCAT GATGAGAAAG AAGAGACTGC TGGGAGTTAC 
GATAGCCGGA ACAAGCATGA GATCATTCGC TGCTTGAAAG CTTTTATGAA CAACAAGTTT 
GGAATCAAGA CCATGTTGGA GACAGAAGAA GGAATCCTAC TGCTGGTCAG AGCCATGGAT 
CCTGCTGTTC CCAACATGAT GATTGATGCA GCTAAGCTGC TTTCTGCTCT TTGTATTCTA 
CCGCAGCCAG AGGACATGAA TGAAAGGGTT TTGGAGGCAA TGACAGAAAG AGCTGAGATG 
GATGAAGTGG AACGTTTCCA GCCGCTGCTG GATGGATTAA AAAGTGGAAC CACTATTGCA 
CTGAAGGTTG GATGCCTACA GCTGATCAAT GCTCTCATCA CACCAGCGGA GGAACTTGAC 
TTCCGAGTTC ACATCAGAAG TGAACTGATG CGTTTGGGGC TACATCAGGT GTTGCAGGAC 
CTTCGAGAGA TTGAAAATGA AGATATGAGA GTGCAACTAA ATGTGTTTGA TGAACAAGGG 
GAAGAGGATT CCTATGACCT GAAGGGACGG CTGGATGACA TTCGCATGGA GATGGATGAC 
TTTAATGAAG TCTTTCAGAT TCTCTTAAAC ACAGTGAAGG ATTCAAAGGC AGAGCCACAC 
TTCCTTTCCA TCCTGCAGCA CTTACTCTTG GTCCGAAATG ACTATGAGGC CAGACCTCAG 
TACTATAAGT TGATTGAAGA ATGTATTTCC CAGATAGTTC TGCACAAGAA CGGGGCTGAT 
CCTGACTTCA AGTGCCGGCA CCTCCAGATT GAGATTGAGG GATTAATTGA TCAAATGATT 
GATAAGACAA AGGTGGAGAA ATCTGAAGCC AAAGCTGCAG AGCTGGAAAA GAAGTTGGAC 
TCAGAGTTAA CAGCCCGACA TGAGCTACAG GTGGAAATGA AAAAGATGGA AAGTGACTTT 
GAGCAGAAGC TTCAAGATCT TCAGGGAGAA AAAGATGCAC TGCATTCTGA AAAGCAGCAA 
ATTGCCACAG AGAAACAGGA CCTGGAAGCA GAGGTGTCCC AGCTCACAGG AGAGGTTGCC 
AAGCTGACAA AGGAACTGGA AGATGCCAAG AAAGAAATGG CTTCCCTCTC TGCGGCAGCT 
ATTACTGTAC CTCCTTCTGT TCCTAGTCGT GCTCCTGTTC CCCCTGCCCC TCCTTTACCT 
GGTGACTCTG GCACTATTAT TCCACCACCA CCTGCTCCTG GGGATAGTAC CACTCCTCCT 
CCTCCTCCTC CTCCTCCTCC TCCTCCACCT CCTTTGCCTG GGGGTGTTTG CATCTCCTCA 
CCCCCTTCTT TACCTGGAGG TACTGCTATC TCTCCACCCC CTCCTTTGTC TGGGGATGCT 
ACCATCCCTC CACCCCCTCC TTTGCCTGAG GGTGTTGGCA TCCCTTCACC CTCTTCTTTG 
CCTGGAGGTA CTGCCATCCC CCCACCTCCT CCTTTGCCTG GGAGTGCTAG AATCCCCCCA 
CCACCACCTC CTTTGCCTGG GAGTGCTGGA ATTCCCCCCC CACCTCCTCC CTTGCCTGGA 
GAAGCAGGAA TGCCACCTCC TCCTCCCCCT CTTCCTGGTG GTCCTGGAAT CCCTCCACCT 
CCTCCATTTC CCGGAGGCCC TGGCATTCCT CCACCTCCAC CCGGAATGGG TATGCCTCCA 
CCTCCCCCAT TTGGATTTGG AGTTCCTGCA GCCCCAGTTC TGCCATTTGG ATTAACCCCC 
AAAAAGCTTT ATAAGCCAGA GGTGCAGCTC CGGAGGCCAA ACTGGTCCAA GCTTGTGGCT 
GAGGACCTCT CCCAGGACTG CTTCTGGACA AAGGTGAAGG AGGACCGCTT TGAGAACAAT 
GAACTTTTCG CCAAACTTAC CCTTACCTTC TCTGCCCAGA CCAAGACTTC CAAAGCCAAG 
AAGGATCAAG AAGGTGGAGA AGAAAAGAAA TCTGTGCAAA AGAAAAAAGT AAAAGAGTTA 
AAGGTGTTGG ATTCAAAGAC AGCCCAGAAT CTCTCAATCT TTTTGGGTTC CTTCCGCATG 
CCCTATCAAG AGATTAAGAA TGTCATCCTG GAGGTGAATG AGGCTGTTCT GACTGAGTCT 
ATGATCCAGA ACCTCATTAA GCAAATGCCA GAGCCAGAGC AGTTAAAAAT GCTTTCTGAA 
CTGAAGGATG AATATGATGA CCTGGCTGAG TCAGAGCAGT TTGGCGTGGT GATGGGCACT 
GTGCCCCGAC TGCGGCCTCG CCTCAATGCC ATTCTCTTCA AGCTACAATT CAGCGAGCAA 
GTGGAGAATA TCAAGCCAGA GATTGTGTCT GTCACTGCTG CATGTGAGGA GTTACGTAAG 
AGTGAGAGCT TTTCCAATCT CCTAGAGATT ACCTTGCTTG TTGGAAATTA CATGAATGCT 
GGCTCCAGAA ATGCTGGTGC TTTTGGCTTC AATATCAGCT TCCTCTGTAA GCTTCGAGAC 
ACCAAGTCCA CAGATCAGAA GATGACGTTG TTACACTTCT TGGCTGAGTT GTGTGAGAAT 
GACTATCCCG ATGTCCTCAA GTTTCCAGAC GAGCTTGCCC ATGTGGAGAA AGCCAGCCGA 
GTTTCTGCTG AAAACTTGCA AAAGAACCTA GATCAGATGA AGAAACAAAT TTCTGATGTG 
GAACGTGATG TTCAGAATTT CCCAGCTGCC ACAGATGAAA AAGACAAGTT TGTTGAAAAA 
ATGACCAGCT TTGTGAAGGA TGCACAGGAA CAGTATAACA AGCTGCGGAT GATGCATTCT 
AACATGGAGA CCCTCTATAA GGAGCTGGGC GAGTACTTCC TCTTTGACCC CAAGAAGTTG 
TCTGTTGAAG AATTTTTCAT GGATCTTCAC AATTTTCGGA ATATGTTTTT GGTGAGCAAG 
GGATACTTGC TGACTGAATC TTTTGTGGGC ATTTCTCACT TTCCCCTGGG TTCCTGTTGG 
CTGGTAGTAG GTATACAATG CCCATGTGTT CCTTCCTCCT CTTCCCTGCC CCAGCAAGCA 
GTCAAGGAGA ACCAGAAGCG GCGGGAGACA GAAGAAAAGA TGAGGCGAGC AAAACTAGCC 
AAGGAGAAGG CAGAGAAGGA GCGGCTAGAG AAGCAGCAGA AGAGAGAGCA ACTCATAGAC 
ATGAATGCAG AGGGCGATGA GACAGGTGTG ATGGACAGTC TTCTAGAAGC CCTGCAGTCA 
GGGGCAGCAT TCCGACGGAA GAGAGGGCCC CGTCAAGCCA ACAGGAAGGC CGGGTGTGCA 
GTCACATCTC TGCTAGCTTC GGAGCTGACC AAGGATGATG CCATGGCTGC TGTTCCTGCC 
AAGGTGTCCA AGAACAGTGA GACATTCCCC ACAATCCTTG AGGAAGCCAA GGAGTTGGTT 
GGCCGTGCAA GCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DIAPH1 ( XM_024454386.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024310154.1 Homo sapiens diaphanous related formin 1 (DIAPH1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHu105639
Clone ID Related Accession (Same CDS sequence)	XM_024454386.1
Accession Version	XM_024454386.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3906bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	protein diaphanous homolog 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAGTAC AGAGTCAGTA TGGTAAGTTA GATAGGAGGA AGACTGATTT CTTCTTTTCA 
TGTCAGAAGA GTCTTGAACT GCTTGTGAAC CAGAGGAACC ATGCCTGTCT GGAGAGATTT 
ACCAGCATGA GAATTAAGAA GGAGAAGGAA AAGCCCAATT CTGCTCATAG AAATTCTTCT 
GCATCATATG GGGATGATCC CACAGCACAG TCATTGCAAG ATGTTTCAGA TGAACAAGTG 
CTGGTTCTCT TTGAACAGAT GCTGCTGGAT ATGAACCTGA ATGAGGAGAA ACAGCAACCT 
TTGAGGGAGA AGGACATCAT CATCAAGAGG GAGATGGTGT CCCAATACTT GTACACCTCC 
AAGGCTGGCA TGAGCCAGAA GGAGAGCTCT AAGTCTGCCA TGATGTATAT TCAGGAGTTG 
AGGTCAGGCT TGCGGGATAT GCCTCTGCTC AGCTGCCTGG AGTCCCTTCG TGTGTCTCTC 
AACAACAACC CTGTCAGTTG GGTGCAAACA TTTGGTGCTG AAGGCTTGGC CTCCTTATTG 
GACATTCTTA AACGACTTCA TGATGAGAAA GAAGAGACTG CTGGGAGTTA CGATAGCCGG 
AACAAGCATG AGATCATTCG CTGCTTGAAA GCTTTTATGA ACAACAAGTT TGGAATCAAG 
ACCATGTTGG AGACAGAAGA AGGAATCCTA CTGCTGGTCA GAGCCATGGA TCCTGCTGTT 
CCCAACATGA TGATTGATGC AGCTAAGCTG CTTTCTGCTC TTTGTATTCT ACCGCAGCCA 
GAGGACATGA ATGAAAGGGT TTTGGAGGCA ATGACAGAAA GAGCTGAGAT GGATGAAGTG 
GAACGTTTCC AGCCGCTGCT GGATGGATTA AAAAGTGGAA CCACTATTGC ACTGAAGGTT 
GGATGCCTAC AGCTGATCAA TGCTCTCATC ACACCAGCGG AGGAACTTGA CTTCCGAGTT 
CACATCAGAA GTGAACTGAT GCGTTTGGGG CTACATCAGG TGTTGCAGGA CCTTCGAGAG 
ATTGAAAATG AAGATATGAG AGTGCAACTA AATGTGTTTG ATGAACAAGG GGAAGAGGAT 
TCCTATGACC TGAAGGGACG GCTGGATGAC ATTCGCATGG AGATGGATGA CTTTAATGAA 
GTCTTTCAGA TTCTCTTAAA CACAGTGAAG GATTCAAAGG CAGAGCCACA CTTCCTTTCC 
ATCCTGCAGC ACTTACTCTT GGTCCGAAAT GACTATGAGG CCAGACCTCA GTACTATAAG 
TTGATTGAAG AATGTATTTC CCAGATAGTT CTGCACAAGA ACGGGGCTGA TCCTGACTTC 
AAGTGCCGGC ACCTCCAGAT TGAGATTGAG GGATTAATTG ATCAAATGAT TGATAAGACA 
AAGGTGGAGA AATCTGAAGC CAAAGCTGCA GAGCTGGAAA AGAAGTTGGA CTCAGAGTTA 
ACAGCCCGAC ATGAGCTACA GGTGGAAATG AAAAAGATGG AAAGTGACTT TGAGCAGAAG 
CTTCAAGATC TTCAGGGAGA AAAAGATGCA CTGCATTCTG AAAAGCAGCA AATTGCCACA 
GAGAAACAGG ACCTGGAAGC AGAGGTGTCC CAGCTCACAG GAGAGGTTGC CAAGCTGACA 
AAGGAACTGG AAGATGCCAA GAAAGAAATG GCTTCCCTCT CTGCGGCAGC TATTACTGTA 
CCTCCTTCTG TTCCTAGTCG TGCTCCTGTT CCCCCTGCCC CTCCTTTACC TGGTGACTCT 
GGCACTATTA TTCCACCACC ACCTGCTCCT GGGGATAGTA CCACTCCTCC TCCTCCTCCT 
CCTCCTCCTC CTCCTCCACC TCCTTTGCCT GGGGGTGTTT GCATCTCCTC ACCCCCTTCT 
TTACCTGGAG GTACTGCTAT CTCTCCACCC CCTCCTTTGT CTGGGGATGC TACCATCCCT 
CCACCCCCTC CTTTGCCTGA GGGTGTTGGC ATCCCTTCAC CCTCTTCTTT GCCTGGAGGT 
ACTGCCATCC CCCCACCTCC TCCTTTGCCT GGGAGTGCTA GAATCCCCCC ACCACCACCT 
CCTTTGCCTG GGAGTGCTGG AATTCCCCCC CCACCTCCTC CCTTGCCTGG AGAAGCAGGA 
ATGCCACCTC CTCCTCCCCC TCTTCCTGGT GGTCCTGGAA TCCCTCCACC TCCTCCATTT 
CCCGGAGGCC CTGGCATTCC TCCACCTCCA CCCGGAATGG GTATGCCTCC ACCTCCCCCA 
TTTGGATTTG GAGTTCCTGC AGCCCCAGTT CTGCCATTTG GATTAACCCC CAAAAAGCTT 
TATAAGCCAG AGGTGCAGCT CCGGAGGCCA AACTGGTCCA AGCTTGTGGC TGAGGACCTC 
TCCCAGGACT GCTTCTGGAC AAAGGTGAAG GAGGACCGCT TTGAGAACAA TGAACTTTTC 
GCCAAACTTA CCCTTACCTT CTCTGCCCAG ACCAAGACTT CCAAAGCCAA GAAGGATCAA 
GAAGGTGGAG AAGAAAAGAA ATCTGTGCAA AAGAAAAAAG TAAAAGAGTT AAAGGTGTTG 
GATTCAAAGA CAGCCCAGAA TCTCTCAATC TTTTTGGGTT CCTTCCGCAT GCCCTATCAA 
GAGATTAAGA ATGTCATCCT GGAGGTGAAT GAGGCTGTTC TGACTGAGTC TATGATCCAG 
AACCTCATTA AGCAAATGCC AGAGCCAGAG CAGTTAAAAA TGCTTTCTGA ACTGAAGGAT 
GAATATGATG ACCTGGCTGA GTCAGAGCAG TTTGGCGTGG TGATGGGCAC TGTGCCCCGA 
CTGCGGCCTC GCCTCAATGC CATTCTCTTC AAGCTACAAT TCAGCGAGCA AGTGGAGAAT 
ATCAAGCCAG AGATTGTGTC TGTCACTGCT GCATGTGAGG AGTTACGTAA GAGTGAGAGC 
TTTTCCAATC TCCTAGAGAT TACCTTGCTT GTTGGAAATT ACATGAATGC TGGCTCCAGA 
AATGCTGGTG CTTTTGGCTT CAATATCAGC TTCCTCTGTA AGCTTCGAGA CACCAAGTCC 
ACAGATCAGA AGATGACGTT GTTACACTTC TTGGCTGAGT TGTGTGAGAA TGACTATCCC 
GATGTCCTCA AGTTTCCAGA CGAGCTTGCC CATGTGGAGA AAGCCAGCCG AGTTTCTGCT 
GAAAACTTGC AAAAGAACCT AGATCAGATG AAGAAACAAA TTTCTGATGT GGAACGTGAT 
GTTCAGAATT TCCCAGCTGC CACAGATGAA AAAGACAAGT TTGTTGAAAA AATGACCAGC 
TTTGTGAAGG ATGCACAGGA ACAGTATAAC AAGCTGCGGA TGATGCATTC TAACATGGAG 
ACCCTCTATA AGGAGCTGGG CGAGTACTTC CTCTTTGACC CCAAGAAGTT GTCTGTTGAA 
GAATTTTTCA TGGATCTTCA CAATTTTCGG AATATGTTTT TGGTGAGCAA GGGATACTTG 
CTGACTGAAT CTTTTGTGGG CATTTCTCAC TTTCCCCTGG GTTCCTGTTG GCTGGTAGTA 
GGTATACAAT GCCCATGTGT TCCTTCCTCC TCTTCCCTGC CCCAGCAAGC AGTCAAGGAG 
AACCAGAAGC GGCGGGAGAC AGAAGAAAAG ATGAGGCGAG CAAAACTAGC CAAGGAGAAG 
GCAGAGAAGG AGCGGCTAGA GAAGCAGCAG AAGAGAGAGC AACTCATAGA CATGAATGCA 
GAGGGCGATG AGACAGGTGT GATGGACAGT CTTCTAGAAG CCCTGCAGTC AGGGGCAGCA 
TTCCGACGGA AGAGAGGGCC CCGTCAAGCC AACAGGAAGG CCGGGTGTGC AGTCACATCT 
CTGCTAGCTT CGGAGCTGAC CAAGGATGAT GCCATGGCTG CTGTTCCTGC CAAGGTGTCC 
AAGAACAGTG AGACATTCCC CACAATCCTT GAGGAAGCCA AGGAGTTGGT TGGCCGTGCA 
AGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024310154.1
CDS	759..4664
Translation	MRVQSQYGKLDRRKTDFFFSCQKSLELLVNQRNHACLERFTSMRIKKEKEKPNSAHRNSSASYGDDPTAQSLQDVSDEQVLVLFEQMLLDMNLNEEKQQPLREKDIIIKREMVSQYLYTSKAGMSQKESSKSAMMYIQELRSGLRDMPLLSCLESLRVSLNNNPVSWVQTFGAEGLASLLDILKRLHDEKEETAGSYDSRNKHEIIRCLKAFMNNKFGIKTMLETEEGILLLVRAMDPAVPNMMIDAAKLLSALCILPQPEDMNERVLEAMTERAEMDEVERFQPLLDGLKSGTTIALKVGCLQLINALITPAEELDFRVHIRSELMRLGLHQVLQDLREIENEDMRVQLNVFDEQGEEDSYDLKGRLDDIRMEMDDFNEVFQILLNTVKDSKAEPHFLSILQHLLLVRNDYEARPQYYKLIEECISQIVLHKNGADPDFKCRHLQIEIEGLIDQMIDKTKVEKSEAKAAELEKKLDSELTARHELQVEMKKMESDFEQKLQDLQGEKDALHSEKQQIATEKQDLEAEVSQLTGEVAKLTKELEDAKKEMASLSAAAITVPPSVPSRAPVPPAPPLPGDSGTIIPPPPAPGDSTTPPPPPPPPPPPPPLPGGVCISSPPSLPGGTAISPPPPLSGDATIPPPPPLPEGVGIPSPSSLPGGTAIPPPPPLPGSARIPPPPPPLPGSAGIPPPPPPLPGEAGMPPPPPPLPGGPGIPPPPPFPGGPGIPPPPPGMGMPPPPPFGFGVPAAPVLPFGLTPKKLYKPEVQLRRPNWSKLVAEDLSQDCFWTKVKEDRFENNELFAKLTLTFSAQTKTSKAKKDQEGGEEKKSVQKKKVKELKVLDSKTAQNLSIFLGSFRMPYQEIKNVILEVNEAVLTESMIQNLIKQMPEPEQLKMLSELKDEYDDLAESEQFGVVMGTVPRLRPRLNAILFKLQFSEQVENIKPEIVSVTAACEELRKSESFSNLLEITLLVGNYMNAGSRNAGAFGFNISFLCKLRDTKSTDQKMTLLHFLAELCENDYPDVLKFPDELAHVEKASRVSAENLQKNLDQMKKQISDVERDVQNFPAATDEKDKFVEKMTSFVKDAQEQYNKLRMMHSNMETLYKELGEYFLFDPKKLSVEEFFMDLHNFRNMFLVSKGYLLTESFVGISHFPLGSCWLVVGIQCPCVPSSSSLPQQAVKENQKRRETEEKMRRAKLAKEKAEKERLEKQQKREQLIDMNAEGDETGVMDSLLEALQSGAAFRRKRGPRQANRKAGCAVTSLLASELTKDDAMAAVPAKVSKNSETFPTILEEAKELVGRAS

Target ORF information:

RefSeq Version	XM_024454386.1
Organism	Homo sapiens(human)
Definition	Homo sapiens diaphanous related formin 1 (DIAPH1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024454386.1

ORF Insert Sequence:

ATGAGAGTAC AGAGTCAGTA TGGTAAGTTA GATAGGAGGA AGACTGATTT CTTCTTTTCA 
TGTCAGAAGA GTCTTGAACT GCTTGTGAAC CAGAGGAACC ATGCCTGTCT GGAGAGATTT 
ACCAGCATGA GAATTAAGAA GGAGAAGGAA AAGCCCAATT CTGCTCATAG AAATTCTTCT 
GCATCATATG GGGATGATCC CACAGCACAG TCATTGCAAG ATGTTTCAGA TGAACAAGTG 
CTGGTTCTCT TTGAACAGAT GCTGCTGGAT ATGAACCTGA ATGAGGAGAA ACAGCAACCT 
TTGAGGGAGA AGGACATCAT CATCAAGAGG GAGATGGTGT CCCAATACTT GTACACCTCC 
AAGGCTGGCA TGAGCCAGAA GGAGAGCTCT AAGTCTGCCA TGATGTATAT TCAGGAGTTG 
AGGTCAGGCT TGCGGGATAT GCCTCTGCTC AGCTGCCTGG AGTCCCTTCG TGTGTCTCTC 
AACAACAACC CTGTCAGTTG GGTGCAAACA TTTGGTGCTG AAGGCTTGGC CTCCTTATTG 
GACATTCTTA AACGACTTCA TGATGAGAAA GAAGAGACTG CTGGGAGTTA CGATAGCCGG 
AACAAGCATG AGATCATTCG CTGCTTGAAA GCTTTTATGA ACAACAAGTT TGGAATCAAG 
ACCATGTTGG AGACAGAAGA AGGAATCCTA CTGCTGGTCA GAGCCATGGA TCCTGCTGTT 
CCCAACATGA TGATTGATGC AGCTAAGCTG CTTTCTGCTC TTTGTATTCT ACCGCAGCCA 
GAGGACATGA ATGAAAGGGT TTTGGAGGCA ATGACAGAAA GAGCTGAGAT GGATGAAGTG 
GAACGTTTCC AGCCGCTGCT GGATGGATTA AAAAGTGGAA CCACTATTGC ACTGAAGGTT 
GGATGCCTAC AGCTGATCAA TGCTCTCATC ACACCAGCGG AGGAACTTGA CTTCCGAGTT 
CACATCAGAA GTGAACTGAT GCGTTTGGGG CTACATCAGG TGTTGCAGGA CCTTCGAGAG 
ATTGAAAATG AAGATATGAG AGTGCAACTA AATGTGTTTG ATGAACAAGG GGAAGAGGAT 
TCCTATGACC TGAAGGGACG GCTGGATGAC ATTCGCATGG AGATGGATGA CTTTAATGAA 
GTCTTTCAGA TTCTCTTAAA CACAGTGAAG GATTCAAAGG CAGAGCCACA CTTCCTTTCC 
ATCCTGCAGC ACTTACTCTT GGTCCGAAAT GACTATGAGG CCAGACCTCA GTACTATAAG 
TTGATTGAAG AATGTATTTC CCAGATAGTT CTGCACAAGA ACGGGGCTGA TCCTGACTTC 
AAGTGCCGGC ACCTCCAGAT TGAGATTGAG GGATTAATTG ATCAAATGAT TGATAAGACA 
AAGGTGGAGA AATCTGAAGC CAAAGCTGCA GAGCTGGAAA AGAAGTTGGA CTCAGAGTTA 
ACAGCCCGAC ATGAGCTACA GGTGGAAATG AAAAAGATGG AAAGTGACTT TGAGCAGAAG 
CTTCAAGATC TTCAGGGAGA AAAAGATGCA CTGCATTCTG AAAAGCAGCA AATTGCCACA 
GAGAAACAGG ACCTGGAAGC AGAGGTGTCC CAGCTCACAG GAGAGGTTGC CAAGCTGACA 
AAGGAACTGG AAGATGCCAA GAAAGAAATG GCTTCCCTCT CTGCGGCAGC TATTACTGTA 
CCTCCTTCTG TTCCTAGTCG TGCTCCTGTT CCCCCTGCCC CTCCTTTACC TGGTGACTCT 
GGCACTATTA TTCCACCACC ACCTGCTCCT GGGGATAGTA CCACTCCTCC TCCTCCTCCT 
CCTCCTCCTC CTCCTCCACC TCCTTTGCCT GGGGGTGTTT GCATCTCCTC ACCCCCTTCT 
TTACCTGGAG GTACTGCTAT CTCTCCACCC CCTCCTTTGT CTGGGGATGC TACCATCCCT 
CCACCCCCTC CTTTGCCTGA GGGTGTTGGC ATCCCTTCAC CCTCTTCTTT GCCTGGAGGT 
ACTGCCATCC CCCCACCTCC TCCTTTGCCT GGGAGTGCTA GAATCCCCCC ACCACCACCT 
CCTTTGCCTG GGAGTGCTGG AATTCCCCCC CCACCTCCTC CCTTGCCTGG AGAAGCAGGA 
ATGCCACCTC CTCCTCCCCC TCTTCCTGGT GGTCCTGGAA TCCCTCCACC TCCTCCATTT 
CCCGGAGGCC CTGGCATTCC TCCACCTCCA CCCGGAATGG GTATGCCTCC ACCTCCCCCA 
TTTGGATTTG GAGTTCCTGC AGCCCCAGTT CTGCCATTTG GATTAACCCC CAAAAAGCTT 
TATAAGCCAG AGGTGCAGCT CCGGAGGCCA AACTGGTCCA AGCTTGTGGC TGAGGACCTC 
TCCCAGGACT GCTTCTGGAC AAAGGTGAAG GAGGACCGCT TTGAGAACAA TGAACTTTTC 
GCCAAACTTA CCCTTACCTT CTCTGCCCAG ACCAAGACTT CCAAAGCCAA GAAGGATCAA 
GAAGGTGGAG AAGAAAAGAA ATCTGTGCAA AAGAAAAAAG TAAAAGAGTT AAAGGTGTTG 
GATTCAAAGA CAGCCCAGAA TCTCTCAATC TTTTTGGGTT CCTTCCGCAT GCCCTATCAA 
GAGATTAAGA ATGTCATCCT GGAGGTGAAT GAGGCTGTTC TGACTGAGTC TATGATCCAG 
AACCTCATTA AGCAAATGCC AGAGCCAGAG CAGTTAAAAA TGCTTTCTGA ACTGAAGGAT 
GAATATGATG ACCTGGCTGA GTCAGAGCAG TTTGGCGTGG TGATGGGCAC TGTGCCCCGA 
CTGCGGCCTC GCCTCAATGC CATTCTCTTC AAGCTACAAT TCAGCGAGCA AGTGGAGAAT 
ATCAAGCCAG AGATTGTGTC TGTCACTGCT GCATGTGAGG AGTTACGTAA GAGTGAGAGC 
TTTTCCAATC TCCTAGAGAT TACCTTGCTT GTTGGAAATT ACATGAATGC TGGCTCCAGA 
AATGCTGGTG CTTTTGGCTT CAATATCAGC TTCCTCTGTA AGCTTCGAGA CACCAAGTCC 
ACAGATCAGA AGATGACGTT GTTACACTTC TTGGCTGAGT TGTGTGAGAA TGACTATCCC 
GATGTCCTCA AGTTTCCAGA CGAGCTTGCC CATGTGGAGA AAGCCAGCCG AGTTTCTGCT 
GAAAACTTGC AAAAGAACCT AGATCAGATG AAGAAACAAA TTTCTGATGT GGAACGTGAT 
GTTCAGAATT TCCCAGCTGC CACAGATGAA AAAGACAAGT TTGTTGAAAA AATGACCAGC 
TTTGTGAAGG ATGCACAGGA ACAGTATAAC AAGCTGCGGA TGATGCATTC TAACATGGAG 
ACCCTCTATA AGGAGCTGGG CGAGTACTTC CTCTTTGACC CCAAGAAGTT GTCTGTTGAA 
GAATTTTTCA TGGATCTTCA CAATTTTCGG AATATGTTTT TGGTGAGCAA GGGATACTTG 
CTGACTGAAT CTTTTGTGGG CATTTCTCAC TTTCCCCTGG GTTCCTGTTG GCTGGTAGTA 
GGTATACAAT GCCCATGTGT TCCTTCCTCC TCTTCCCTGC CCCAGCAAGC AGTCAAGGAG 
AACCAGAAGC GGCGGGAGAC AGAAGAAAAG ATGAGGCGAG CAAAACTAGC CAAGGAGAAG 
GCAGAGAAGG AGCGGCTAGA GAAGCAGCAG AAGAGAGAGC AACTCATAGA CATGAATGCA 
GAGGGCGATG AGACAGGTGT GATGGACAGT CTTCTAGAAG CCCTGCAGTC AGGGGCAGCA 
TTCCGACGGA AGAGAGGGCC CCGTCAAGCC AACAGGAAGG CCGGGTGTGC AGTCACATCT 
CTGCTAGCTT CGGAGCTGAC CAAGGATGAT GCCATGGCTG CTGTTCCTGC CAAGGTGTCC 
AAGAACAGTG AGACATTCCC CACAATCCTT GAGGAAGCCA AGGAGTTGGT TGGCCGTGCA 
AGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DIAPH1 ( XM_024454387.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024310155.1 Homo sapiens diaphanous related formin 1 (DIAPH1), transcript variant X4, mRNA.

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CloneID	OHu105640
Clone ID Related Accession (Same CDS sequence)	XM_024454387.1
Accession Version	XM_024454387.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3876bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	protein diaphanous homolog 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGATAA ACCTCTCCTG TGGGAAGAAA GTTGGACTGC GAGTTCAAGA AACTCTGAAG 
CGGCTCATGG CAGATGAGCT GGAGAGATTT ACCAGCATGA GAATTAAGAA GGAGAAGGAA 
AAGCCCAATT CTGCTCATAG AAATTCTTCT GCATCATATG GGGATGATCC CACAGCACAG 
TCATTGCAAG ATGTTTCAGA TGAACAAGTG CTGGTTCTCT TTGAACAGAT GCTGCTGGAT 
ATGAACCTGA ATGAGGAGAA ACAGCAACCT TTGAGGGAGA AGGACATCAT CATCAAGAGG 
GAGATGGTGT CCCAATACTT GTACACCTCC AAGGCTGGCA TGAGCCAGAA GGAGAGCTCT 
AAGTCTGCCA TGATGTATAT TCAGGAGTTG AGGTCAGGCT TGCGGGATAT GCCTCTGCTC 
AGCTGCCTGG AGTCCCTTCG TGTGTCTCTC AACAACAACC CTGTCAGTTG GGTGCAAACA 
TTTGGTGCTG AAGGCTTGGC CTCCTTATTG GACATTCTTA AACGACTTCA TGATGAGAAA 
GAAGAGACTG CTGGGAGTTA CGATAGCCGG AACAAGCATG AGATCATTCG CTGCTTGAAA 
GCTTTTATGA ACAACAAGTT TGGAATCAAG ACCATGTTGG AGACAGAAGA AGGAATCCTA 
CTGCTGGTCA GAGCCATGGA TCCTGCTGTT CCCAACATGA TGATTGATGC AGCTAAGCTG 
CTTTCTGCTC TTTGTATTCT ACCGCAGCCA GAGGACATGA ATGAAAGGGT TTTGGAGGCA 
ATGACAGAAA GAGCTGAGAT GGATGAAGTG GAACGTTTCC AGCCGCTGCT GGATGGATTA 
AAAAGTGGAA CCACTATTGC ACTGAAGGTT GGATGCCTAC AGCTGATCAA TGCTCTCATC 
ACACCAGCGG AGGAACTTGA CTTCCGAGTT CACATCAGAA GTGAACTGAT GCGTTTGGGG 
CTACATCAGG TGTTGCAGGA CCTTCGAGAG ATTGAAAATG AAGATATGAG AGTGCAACTA 
AATGTGTTTG ATGAACAAGG GGAAGAGGAT TCCTATGACC TGAAGGGACG GCTGGATGAC 
ATTCGCATGG AGATGGATGA CTTTAATGAA GTCTTTCAGA TTCTCTTAAA CACAGTGAAG 
GATTCAAAGG CAGAGCCACA CTTCCTTTCC ATCCTGCAGC ACTTACTCTT GGTCCGAAAT 
GACTATGAGG CCAGACCTCA GTACTATAAG TTGATTGAAG AATGTATTTC CCAGATAGTT 
CTGCACAAGA ACGGGGCTGA TCCTGACTTC AAGTGCCGGC ACCTCCAGAT TGAGATTGAG 
GGATTAATTG ATCAAATGAT TGATAAGACA AAGGTGGAGA AATCTGAAGC CAAAGCTGCA 
GAGCTGGAAA AGAAGTTGGA CTCAGAGTTA ACAGCCCGAC ATGAGCTACA GGTGGAAATG 
AAAAAGATGG AAAGTGACTT TGAGCAGAAG CTTCAAGATC TTCAGGGAGA AAAAGATGCA 
CTGCATTCTG AAAAGCAGCA AATTGCCACA GAGAAACAGG ACCTGGAAGC AGAGGTGTCC 
CAGCTCACAG GAGAGGTTGC CAAGCTGACA AAGGAACTGG AAGATGCCAA GAAAGAAATG 
GCTTCCCTCT CTGCGGCAGC TATTACTGTA CCTCCTTCTG TTCCTAGTCG TGCTCCTGTT 
CCCCCTGCCC CTCCTTTACC TGGTGACTCT GGCACTATTA TTCCACCACC ACCTGCTCCT 
GGGGATAGTA CCACTCCTCC TCCTCCTCCT CCTCCTCCTC CTCCTCCACC TCCTTTGCCT 
GGGGGTGTTT GCATCTCCTC ACCCCCTTCT TTACCTGGAG GTACTGCTAT CTCTCCACCC 
CCTCCTTTGT CTGGGGATGC TACCATCCCT CCACCCCCTC CTTTGCCTGA GGGTGTTGGC 
ATCCCTTCAC CCTCTTCTTT GCCTGGAGGT ACTGCCATCC CCCCACCTCC TCCTTTGCCT 
GGGAGTGCTA GAATCCCCCC ACCACCACCT CCTTTGCCTG GGAGTGCTGG AATTCCCCCC 
CCACCTCCTC CCTTGCCTGG AGAAGCAGGA ATGCCACCTC CTCCTCCCCC TCTTCCTGGT 
GGTCCTGGAA TCCCTCCACC TCCTCCATTT CCCGGAGGCC CTGGCATTCC TCCACCTCCA 
CCCGGAATGG GTATGCCTCC ACCTCCCCCA TTTGGATTTG GAGTTCCTGC AGCCCCAGTT 
CTGCCATTTG GATTAACCCC CAAAAAGCTT TATAAGCCAG AGGTGCAGCT CCGGAGGCCA 
AACTGGTCCA AGCTTGTGGC TGAGGACCTC TCCCAGGACT GCTTCTGGAC AAAGGTGAAG 
GAGGACCGCT TTGAGAACAA TGAACTTTTC GCCAAACTTA CCCTTACCTT CTCTGCCCAG 
ACCAAGACTT CCAAAGCCAA GAAGGATCAA GAAGGTGGAG AAGAAAAGAA ATCTGTGCAA 
AAGAAAAAAG TAAAAGAGTT AAAGGTGTTG GATTCAAAGA CAGCCCAGAA TCTCTCAATC 
TTTTTGGGTT CCTTCCGCAT GCCCTATCAA GAGATTAAGA ATGTCATCCT GGAGGTGAAT 
GAGGCTGTTC TGACTGAGTC TATGATCCAG AACCTCATTA AGCAAATGCC AGAGCCAGAG 
CAGTTAAAAA TGCTTTCTGA ACTGAAGGAT GAATATGATG ACCTGGCTGA GTCAGAGCAG 
TTTGGCGTGG TGATGGGCAC TGTGCCCCGA CTGCGGCCTC GCCTCAATGC CATTCTCTTC 
AAGCTACAAT TCAGCGAGCA AGTGGAGAAT ATCAAGCCAG AGATTGTGTC TGTCACTGCT 
GCATGTGAGG AGTTACGTAA GAGTGAGAGC TTTTCCAATC TCCTAGAGAT TACCTTGCTT 
GTTGGAAATT ACATGAATGC TGGCTCCAGA AATGCTGGTG CTTTTGGCTT CAATATCAGC 
TTCCTCTGTA AGCTTCGAGA CACCAAGTCC ACAGATCAGA AGATGACGTT GTTACACTTC 
TTGGCTGAGT TGTGTGAGAA TGACTATCCC GATGTCCTCA AGTTTCCAGA CGAGCTTGCC 
CATGTGGAGA AAGCCAGCCG AGTTTCTGCT GAAAACTTGC AAAAGAACCT AGATCAGATG 
AAGAAACAAA TTTCTGATGT GGAACGTGAT GTTCAGAATT TCCCAGCTGC CACAGATGAA 
AAAGACAAGT TTGTTGAAAA AATGACCAGC TTTGTGAAGG ATGCACAGGA ACAGTATAAC 
AAGCTGCGGA TGATGCATTC TAACATGGAG ACCCTCTATA AGGAGCTGGG CGAGTACTTC 
CTCTTTGACC CCAAGAAGTT GTCTGTTGAA GAATTTTTCA TGGATCTTCA CAATTTTCGG 
AATATGTTTT TGGTGAGCAA GGGATACTTG CTGACTGAAT CTTTTGTGGG CATTTCTCAC 
TTTCCCCTGG GTTCCTGTTG GCTGGTAGTA GGTATACAAT GCCCATGTGT TCCTTCCTCC 
TCTTCCCTGC CCCAGCAAGC AGTCAAGGAG AACCAGAAGC GGCGGGAGAC AGAAGAAAAG 
ATGAGGCGAG CAAAACTAGC CAAGGAGAAG GCAGAGAAGG AGCGGCTAGA GAAGCAGCAG 
AAGAGAGAGC AACTCATAGA CATGAATGCA GAGGGCGATG AGACAGGTGT GATGGACAGT 
CTTCTAGAAG CCCTGCAGTC AGGGGCAGCA TTCCGACGGA AGAGAGGGCC CCGTCAAGCC 
AACAGGAAGG CCGGGTGTGC AGTCACATCT CTGCTAGCTT CGGAGCTGAC CAAGGATGAT 
GCCATGGCTG CTGTTCCTGC CAAGGTGTCC AAGAACAGTG AGACATTCCC CACAATCCTT 
GAGGAAGCCA AGGAGTTGGT TGGCCGTGCA AGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024310155.1
CDS	16..3891
Translation	MVINLSCGKKVGLRVQETLKRLMADELERFTSMRIKKEKEKPNSAHRNSSASYGDDPTAQSLQDVSDEQVLVLFEQMLLDMNLNEEKQQPLREKDIIIKREMVSQYLYTSKAGMSQKESSKSAMMYIQELRSGLRDMPLLSCLESLRVSLNNNPVSWVQTFGAEGLASLLDILKRLHDEKEETAGSYDSRNKHEIIRCLKAFMNNKFGIKTMLETEEGILLLVRAMDPAVPNMMIDAAKLLSALCILPQPEDMNERVLEAMTERAEMDEVERFQPLLDGLKSGTTIALKVGCLQLINALITPAEELDFRVHIRSELMRLGLHQVLQDLREIENEDMRVQLNVFDEQGEEDSYDLKGRLDDIRMEMDDFNEVFQILLNTVKDSKAEPHFLSILQHLLLVRNDYEARPQYYKLIEECISQIVLHKNGADPDFKCRHLQIEIEGLIDQMIDKTKVEKSEAKAAELEKKLDSELTARHELQVEMKKMESDFEQKLQDLQGEKDALHSEKQQIATEKQDLEAEVSQLTGEVAKLTKELEDAKKEMASLSAAAITVPPSVPSRAPVPPAPPLPGDSGTIIPPPPAPGDSTTPPPPPPPPPPPPPLPGGVCISSPPSLPGGTAISPPPPLSGDATIPPPPPLPEGVGIPSPSSLPGGTAIPPPPPLPGSARIPPPPPPLPGSAGIPPPPPPLPGEAGMPPPPPPLPGGPGIPPPPPFPGGPGIPPPPPGMGMPPPPPFGFGVPAAPVLPFGLTPKKLYKPEVQLRRPNWSKLVAEDLSQDCFWTKVKEDRFENNELFAKLTLTFSAQTKTSKAKKDQEGGEEKKSVQKKKVKELKVLDSKTAQNLSIFLGSFRMPYQEIKNVILEVNEAVLTESMIQNLIKQMPEPEQLKMLSELKDEYDDLAESEQFGVVMGTVPRLRPRLNAILFKLQFSEQVENIKPEIVSVTAACEELRKSESFSNLLEITLLVGNYMNAGSRNAGAFGFNISFLCKLRDTKSTDQKMTLLHFLAELCENDYPDVLKFPDELAHVEKASRVSAENLQKNLDQMKKQISDVERDVQNFPAATDEKDKFVEKMTSFVKDAQEQYNKLRMMHSNMETLYKELGEYFLFDPKKLSVEEFFMDLHNFRNMFLVSKGYLLTESFVGISHFPLGSCWLVVGIQCPCVPSSSSLPQQAVKENQKRRETEEKMRRAKLAKEKAEKERLEKQQKREQLIDMNAEGDETGVMDSLLEALQSGAAFRRKRGPRQANRKAGCAVTSLLASELTKDDAMAAVPAKVSKNSETFPTILEEAKELVGRAS

Target ORF information:

RefSeq Version	XM_024454387.1
Organism	Homo sapiens(human)
Definition	Homo sapiens diaphanous related formin 1 (DIAPH1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024454387.1

ORF Insert Sequence:

ATGGTGATAA ACCTCTCCTG TGGGAAGAAA GTTGGACTGC GAGTTCAAGA AACTCTGAAG 
CGGCTCATGG CAGATGAGCT GGAGAGATTT ACCAGCATGA GAATTAAGAA GGAGAAGGAA 
AAGCCCAATT CTGCTCATAG AAATTCTTCT GCATCATATG GGGATGATCC CACAGCACAG 
TCATTGCAAG ATGTTTCAGA TGAACAAGTG CTGGTTCTCT TTGAACAGAT GCTGCTGGAT 
ATGAACCTGA ATGAGGAGAA ACAGCAACCT TTGAGGGAGA AGGACATCAT CATCAAGAGG 
GAGATGGTGT CCCAATACTT GTACACCTCC AAGGCTGGCA TGAGCCAGAA GGAGAGCTCT 
AAGTCTGCCA TGATGTATAT TCAGGAGTTG AGGTCAGGCT TGCGGGATAT GCCTCTGCTC 
AGCTGCCTGG AGTCCCTTCG TGTGTCTCTC AACAACAACC CTGTCAGTTG GGTGCAAACA 
TTTGGTGCTG AAGGCTTGGC CTCCTTATTG GACATTCTTA AACGACTTCA TGATGAGAAA 
GAAGAGACTG CTGGGAGTTA CGATAGCCGG AACAAGCATG AGATCATTCG CTGCTTGAAA 
GCTTTTATGA ACAACAAGTT TGGAATCAAG ACCATGTTGG AGACAGAAGA AGGAATCCTA 
CTGCTGGTCA GAGCCATGGA TCCTGCTGTT CCCAACATGA TGATTGATGC AGCTAAGCTG 
CTTTCTGCTC TTTGTATTCT ACCGCAGCCA GAGGACATGA ATGAAAGGGT TTTGGAGGCA 
ATGACAGAAA GAGCTGAGAT GGATGAAGTG GAACGTTTCC AGCCGCTGCT GGATGGATTA 
AAAAGTGGAA CCACTATTGC ACTGAAGGTT GGATGCCTAC AGCTGATCAA TGCTCTCATC 
ACACCAGCGG AGGAACTTGA CTTCCGAGTT CACATCAGAA GTGAACTGAT GCGTTTGGGG 
CTACATCAGG TGTTGCAGGA CCTTCGAGAG ATTGAAAATG AAGATATGAG AGTGCAACTA 
AATGTGTTTG ATGAACAAGG GGAAGAGGAT TCCTATGACC TGAAGGGACG GCTGGATGAC 
ATTCGCATGG AGATGGATGA CTTTAATGAA GTCTTTCAGA TTCTCTTAAA CACAGTGAAG 
GATTCAAAGG CAGAGCCACA CTTCCTTTCC ATCCTGCAGC ACTTACTCTT GGTCCGAAAT 
GACTATGAGG CCAGACCTCA GTACTATAAG TTGATTGAAG AATGTATTTC CCAGATAGTT 
CTGCACAAGA ACGGGGCTGA TCCTGACTTC AAGTGCCGGC ACCTCCAGAT TGAGATTGAG 
GGATTAATTG ATCAAATGAT TGATAAGACA AAGGTGGAGA AATCTGAAGC CAAAGCTGCA 
GAGCTGGAAA AGAAGTTGGA CTCAGAGTTA ACAGCCCGAC ATGAGCTACA GGTGGAAATG 
AAAAAGATGG AAAGTGACTT TGAGCAGAAG CTTCAAGATC TTCAGGGAGA AAAAGATGCA 
CTGCATTCTG AAAAGCAGCA AATTGCCACA GAGAAACAGG ACCTGGAAGC AGAGGTGTCC 
CAGCTCACAG GAGAGGTTGC CAAGCTGACA AAGGAACTGG AAGATGCCAA GAAAGAAATG 
GCTTCCCTCT CTGCGGCAGC TATTACTGTA CCTCCTTCTG TTCCTAGTCG TGCTCCTGTT 
CCCCCTGCCC CTCCTTTACC TGGTGACTCT GGCACTATTA TTCCACCACC ACCTGCTCCT 
GGGGATAGTA CCACTCCTCC TCCTCCTCCT CCTCCTCCTC CTCCTCCACC TCCTTTGCCT 
GGGGGTGTTT GCATCTCCTC ACCCCCTTCT TTACCTGGAG GTACTGCTAT CTCTCCACCC 
CCTCCTTTGT CTGGGGATGC TACCATCCCT CCACCCCCTC CTTTGCCTGA GGGTGTTGGC 
ATCCCTTCAC CCTCTTCTTT GCCTGGAGGT ACTGCCATCC CCCCACCTCC TCCTTTGCCT 
GGGAGTGCTA GAATCCCCCC ACCACCACCT CCTTTGCCTG GGAGTGCTGG AATTCCCCCC 
CCACCTCCTC CCTTGCCTGG AGAAGCAGGA ATGCCACCTC CTCCTCCCCC TCTTCCTGGT 
GGTCCTGGAA TCCCTCCACC TCCTCCATTT CCCGGAGGCC CTGGCATTCC TCCACCTCCA 
CCCGGAATGG GTATGCCTCC ACCTCCCCCA TTTGGATTTG GAGTTCCTGC AGCCCCAGTT 
CTGCCATTTG GATTAACCCC CAAAAAGCTT TATAAGCCAG AGGTGCAGCT CCGGAGGCCA 
AACTGGTCCA AGCTTGTGGC TGAGGACCTC TCCCAGGACT GCTTCTGGAC AAAGGTGAAG 
GAGGACCGCT TTGAGAACAA TGAACTTTTC GCCAAACTTA CCCTTACCTT CTCTGCCCAG 
ACCAAGACTT CCAAAGCCAA GAAGGATCAA GAAGGTGGAG AAGAAAAGAA ATCTGTGCAA 
AAGAAAAAAG TAAAAGAGTT AAAGGTGTTG GATTCAAAGA CAGCCCAGAA TCTCTCAATC 
TTTTTGGGTT CCTTCCGCAT GCCCTATCAA GAGATTAAGA ATGTCATCCT GGAGGTGAAT 
GAGGCTGTTC TGACTGAGTC TATGATCCAG AACCTCATTA AGCAAATGCC AGAGCCAGAG 
CAGTTAAAAA TGCTTTCTGA ACTGAAGGAT GAATATGATG ACCTGGCTGA GTCAGAGCAG 
TTTGGCGTGG TGATGGGCAC TGTGCCCCGA CTGCGGCCTC GCCTCAATGC CATTCTCTTC 
AAGCTACAAT TCAGCGAGCA AGTGGAGAAT ATCAAGCCAG AGATTGTGTC TGTCACTGCT 
GCATGTGAGG AGTTACGTAA GAGTGAGAGC TTTTCCAATC TCCTAGAGAT TACCTTGCTT 
GTTGGAAATT ACATGAATGC TGGCTCCAGA AATGCTGGTG CTTTTGGCTT CAATATCAGC 
TTCCTCTGTA AGCTTCGAGA CACCAAGTCC ACAGATCAGA AGATGACGTT GTTACACTTC 
TTGGCTGAGT TGTGTGAGAA TGACTATCCC GATGTCCTCA AGTTTCCAGA CGAGCTTGCC 
CATGTGGAGA AAGCCAGCCG AGTTTCTGCT GAAAACTTGC AAAAGAACCT AGATCAGATG 
AAGAAACAAA TTTCTGATGT GGAACGTGAT GTTCAGAATT TCCCAGCTGC CACAGATGAA 
AAAGACAAGT TTGTTGAAAA AATGACCAGC TTTGTGAAGG ATGCACAGGA ACAGTATAAC 
AAGCTGCGGA TGATGCATTC TAACATGGAG ACCCTCTATA AGGAGCTGGG CGAGTACTTC 
CTCTTTGACC CCAAGAAGTT GTCTGTTGAA GAATTTTTCA TGGATCTTCA CAATTTTCGG 
AATATGTTTT TGGTGAGCAA GGGATACTTG CTGACTGAAT CTTTTGTGGG CATTTCTCAC 
TTTCCCCTGG GTTCCTGTTG GCTGGTAGTA GGTATACAAT GCCCATGTGT TCCTTCCTCC 
TCTTCCCTGC CCCAGCAAGC AGTCAAGGAG AACCAGAAGC GGCGGGAGAC AGAAGAAAAG 
ATGAGGCGAG CAAAACTAGC CAAGGAGAAG GCAGAGAAGG AGCGGCTAGA GAAGCAGCAG 
AAGAGAGAGC AACTCATAGA CATGAATGCA GAGGGCGATG AGACAGGTGT GATGGACAGT 
CTTCTAGAAG CCCTGCAGTC AGGGGCAGCA TTCCGACGGA AGAGAGGGCC CCGTCAAGCC 
AACAGGAAGG CCGGGTGTGC AGTCACATCT CTGCTAGCTT CGGAGCTGAC CAAGGATGAT 
GCCATGGCTG CTGTTCCTGC CAAGGTGTCC AAGAACAGTG AGACATTCCC CACAATCCTT 
GAGGAAGCCA AGGAGTTGGT TGGCCGTGCA AGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DIAPH1 ( NM_001079812.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001073280.1 Homo sapiens diaphanous related formin 1 (DIAPH1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu26133
Clone ID Related Accession (Same CDS sequence)	NM_001079812.2 , NM_001079812.3
Accession Version	NM_001079812.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3792bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-19
Organism	Homo sapiens(human)
Product	protein diaphanous homolog 1 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA593734.1, BC117257.1, AK023345.1 and AY007129.1. This sequence is a reference standard in the RefSeqGene project. On May 24, 2008 this sequence version replaced NM_001079812.1. Transcript Variant: This variant (2) lacks an in-frame exon in the 5' coding region, as compared to variant 1, which results in a shorter isoform (2). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: DQ067453.1, BC117257.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGCCGC CCGGCGGGAG CCTGGGGCCC GGCCGCGGGA CCCGGGACAA GAAGAAGGGC 
CGGAGCCCAG ATGAGCTGCC CTCGGCGGGC GGCGACGGCG GCAAATCTAA GAAATTTCTG 
GAGAGATTTA CCAGCATGAG AATTAAGAAG GAGAAGGAAA AGCCCAATTC TGCTCATAGA 
AATTCTTCTG CATCATATGG GGATGATCCC ACAGCACAGT CATTGCAAGA TGTTTCAGAT 
GAACAAGTGC TGGTTCTCTT TGAACAGATG CTGCTGGATA TGAACCTGAA TGAGGAGAAA 
CAGCAACCTT TGAGGGAGAA GGACATCATC ATCAAGAGGG AGATGGTGTC CCAATACTTG 
TACACCTCCA AGGCTGGCAT GAGCCAGAAG GAGAGCTCTA AGTCTGCCAT GATGTATATT 
CAGGAGTTGA GGTCAGGCTT GCGGGATATG CCTCTGCTCA GCTGCCTGGA GTCCCTTCGT 
GTGTCTCTCA ACAACAACCC TGTCAGTTGG GTGCAAACAT TTGGTGCTGA AGGCTTGGCC 
TCCTTATTGG ACATTCTTAA ACGACTTCAT GATGAGAAAG AAGAGACTGC TGGGAGTTAC 
GATAGCCGGA ACAAGCATGA GATCATTCGC TGCTTGAAAG CTTTTATGAA CAACAAGTTT 
GGAATCAAGA CCATGTTGGA GACAGAAGAA GGAATCCTAC TGCTGGTCAG AGCCATGGAT 
CCTGCTGTTC CCAACATGAT GATTGATGCA GCTAAGCTGC TTTCTGCTCT TTGTATTCTA 
CCGCAGCCAG AGGACATGAA TGAAAGGGTT TTGGAGGCAA TGACAGAAAG AGCTGAGATG 
GATGAAGTGG AACGTTTCCA GCCGCTGCTG GATGGATTAA AAAGTGGAAC CACTATTGCA 
CTGAAGGTTG GATGCCTACA GCTGATCAAT GCTCTCATCA CACCAGCGGA GGAACTTGAC 
TTCCGAGTTC ACATCAGAAG TGAACTGATG CGTTTGGGGC TACATCAGGT GTTGCAGGAC 
CTTCGAGAGA TTGAAAATGA AGATATGAGA GTGCAACTAA ATGTGTTTGA TGAACAAGGG 
GAAGAGGATT CCTATGACCT GAAGGGACGG CTGGATGACA TTCGCATGGA GATGGATGAC 
TTTAATGAAG TCTTTCAGAT TCTCTTAAAC ACAGTGAAGG ATTCAAAGGC AGAGCCACAC 
TTCCTTTCCA TCCTGCAGCA CTTACTCTTG GTCCGAAATG ACTATGAGGC CAGACCTCAG 
TACTATAAGT TGATTGAAGA ATGTATTTCC CAGATAGTTC TGCACAAGAA CGGGGCTGAT 
CCTGACTTCA AGTGCCGGCA CCTCCAGATT GAGATTGAGG GATTAATTGA TCAAATGATT 
GATAAGACAA AGGTGGAGAA ATCTGAAGCC AAAGCTGCAG AGCTGGAAAA GAAGTTGGAC 
TCAGAGTTAA CAGCCCGACA TGAGCTACAG GTGGAAATGA AAAAGATGGA AAGTGACTTT 
GAGCAGAAGC TTCAAGATCT TCAGGGAGAA AAAGATGCAC TGCATTCTGA AAAGCAGCAA 
ATTGCCACAG AGAAACAGGA CCTGGAAGCA GAGGTGTCCC AGCTCACAGG AGAGGTTGCC 
AAGCTGACAA AGGAACTGGA AGATGCCAAG AAAGAAATGG CTTCCCTCTC TGCGGCAGCT 
ATTACTGTAC CTCCTTCTGT TCCTAGTCGT GCTCCTGTTC CCCCTGCCCC TCCTTTACCT 
GGTGACTCTG GCACTATTAT TCCACCACCA CCTGCTCCTG GGGATAGTAC CACTCCTCCT 
CCTCCTCCTC CTCCTCCTCC TCCTCCACCT CCTTTGCCTG GGGGTGTTTG CATCTCCTCA 
CCCCCTTCTT TACCTGGAGG TACTGCTATC TCTCCACCCC CTCCTTTGTC TGGGGATGCT 
ACCATCCCTC CACCCCCTCC TTTGCCTGAG GGTGTTGGCA TCCCTTCACC CTCTTCTTTG 
CCTGGAGGTA CTGCCATCCC CCCACCTCCT CCTTTGCCTG GGAGTGCTAG AATCCCCCCA 
CCACCACCTC CTTTGCCTGG GAGTGCTGGA ATTCCCCCCC CACCTCCTCC CTTGCCTGGA 
GAAGCAGGAA TGCCACCTCC TCCTCCCCCT CTTCCTGGTG GTCCTGGAAT CCCTCCACCT 
CCTCCATTTC CCGGAGGCCC TGGCATTCCT CCACCTCCAC CCGGAATGGG TATGCCTCCA 
CCTCCCCCAT TTGGATTTGG AGTTCCTGCA GCCCCAGTTC TGCCATTTGG ATTAACCCCC 
AAAAAGCTTT ATAAGCCAGA GGTGCAGCTC CGGAGGCCAA ACTGGTCCAA GCTTGTGGCT 
GAGGACCTCT CCCAGGACTG CTTCTGGACA AAGGTGAAGG AGGACCGCTT TGAGAACAAT 
GAACTTTTCG CCAAACTTAC CCTTACCTTC TCTGCCCAGA CCAAGACTTC CAAAGCCAAG 
AAGGATCAAG AAGGTGGAGA AGAAAAGAAA TCTGTGCAAA AGAAAAAAGT AAAAGAGTTA 
AAGGTGTTGG ATTCAAAGAC AGCCCAGAAT CTCTCAATCT TTTTGGGTTC CTTCCGCATG 
CCCTATCAAG AGATTAAGAA TGTCATCCTG GAGGTGAATG AGGCTGTTCT GACTGAGTCT 
ATGATCCAGA ACCTCATTAA GCAAATGCCA GAGCCAGAGC AGTTAAAAAT GCTTTCTGAA 
CTGAAGGATG AATATGATGA CCTGGCTGAG TCAGAGCAGT TTGGCGTGGT GATGGGCACT 
GTGCCCCGAC TGCGGCCTCG CCTCAATGCC ATTCTCTTCA AGCTACAATT CAGCGAGCAA 
GTGGAGAATA TCAAGCCAGA GATTGTGTCT GTCACTGCTG CATGTGAGGA GTTACGTAAG 
AGTGAGAGCT TTTCCAATCT CCTAGAGATT ACCTTGCTTG TTGGAAATTA CATGAATGCT 
GGCTCCAGAA ATGCTGGTGC TTTTGGCTTC AATATCAGCT TCCTCTGTAA GCTTCGAGAC 
ACCAAGTCCA CAGATCAGAA GATGACGTTG TTACACTTCT TGGCTGAGTT GTGTGAGAAT 
GACTATCCCG ATGTCCTCAA GTTTCCAGAC GAGCTTGCCC ATGTGGAGAA AGCCAGCCGA 
GTTTCTGCTG AAAACTTGCA AAAGAACCTA GATCAGATGA AGAAACAAAT TTCTGATGTG 
GAACGTGATG TTCAGAATTT CCCAGCTGCC ACAGATGAAA AAGACAAGTT TGTTGAAAAA 
ATGACCAGCT TTGTGAAGGA TGCACAGGAA CAGTATAACA AGCTGCGGAT GATGCATTCT 
AACATGGAGA CCCTCTATAA GGAGCTGGGC GAGTACTTCC TCTTTGACCC CAAGAAGTTG 
TCTGTTGAAG AATTTTTCAT GGATCTTCAC AATTTTCGGA ATATGTTTTT GCAAGCAGTC 
AAGGAGAACC AGAAGCGGCG GGAGACAGAA GAAAAGATGA GGCGAGCAAA ACTAGCCAAG 
GAGAAGGCAG AGAAGGAGCG GCTAGAGAAG CAGCAGAAGA GAGAGCAACT CATAGACATG 
AATGCAGAGG GCGATGAGAC AGGTGTGATG GACAGTCTTC TAGAAGCCCT GCAGTCAGGG 
GCAGCATTCC GACGGAAGAG AGGGCCCCGT CAAGCCAACA GGAAGGCCGG GTGTGCAGTC 
ACATCTCTGC TAGCTTCGGA GCTGACCAAG GATGATGCCA TGGCTGCTGT TCCTGCCAAG 
GTGTCCAAGA ACAGTGAGAC ATTCCCCACA ATCCTTGAGG AAGCCAAGGA GTTGGTTGGC 
CGTGCAAGCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001073280.1
CDS	142..3933
Translation	MEPPGGSLGPGRGTRDKKKGRSPDELPSAGGDGGKSKKFLERFTSMRIKKEKEKPNSAHRNSSASYGDDPTAQSLQDVSDEQVLVLFEQMLLDMNLNEEKQQPLREKDIIIKREMVSQYLYTSKAGMSQKESSKSAMMYIQELRSGLRDMPLLSCLESLRVSLNNNPVSWVQTFGAEGLASLLDILKRLHDEKEETAGSYDSRNKHEIIRCLKAFMNNKFGIKTMLETEEGILLLVRAMDPAVPNMMIDAAKLLSALCILPQPEDMNERVLEAMTERAEMDEVERFQPLLDGLKSGTTIALKVGCLQLINALITPAEELDFRVHIRSELMRLGLHQVLQDLREIENEDMRVQLNVFDEQGEEDSYDLKGRLDDIRMEMDDFNEVFQILLNTVKDSKAEPHFLSILQHLLLVRNDYEARPQYYKLIEECISQIVLHKNGADPDFKCRHLQIEIEGLIDQMIDKTKVEKSEAKAAELEKKLDSELTARHELQVEMKKMESDFEQKLQDLQGEKDALHSEKQQIATEKQDLEAEVSQLTGEVAKLTKELEDAKKEMASLSAAAITVPPSVPSRAPVPPAPPLPGDSGTIIPPPPAPGDSTTPPPPPPPPPPPPPLPGGVCISSPPSLPGGTAISPPPPLSGDATIPPPPPLPEGVGIPSPSSLPGGTAIPPPPPLPGSARIPPPPPPLPGSAGIPPPPPPLPGEAGMPPPPPPLPGGPGIPPPPPFPGGPGIPPPPPGMGMPPPPPFGFGVPAAPVLPFGLTPKKLYKPEVQLRRPNWSKLVAEDLSQDCFWTKVKEDRFENNELFAKLTLTFSAQTKTSKAKKDQEGGEEKKSVQKKKVKELKVLDSKTAQNLSIFLGSFRMPYQEIKNVILEVNEAVLTESMIQNLIKQMPEPEQLKMLSELKDEYDDLAESEQFGVVMGTVPRLRPRLNAILFKLQFSEQVENIKPEIVSVTAACEELRKSESFSNLLEITLLVGNYMNAGSRNAGAFGFNISFLCKLRDTKSTDQKMTLLHFLAELCENDYPDVLKFPDELAHVEKASRVSAENLQKNLDQMKKQISDVERDVQNFPAATDEKDKFVEKMTSFVKDAQEQYNKLRMMHSNMETLYKELGEYFLFDPKKLSVEEFFMDLHNFRNMFLQAVKENQKRRETEEKMRRAKLAKEKAEKERLEKQQKREQLIDMNAEGDETGVMDSLLEALQSGAAFRRKRGPRQANRKAGCAVTSLLASELTKDDAMAAVPAKVSKNSETFPTILEEAKELVGRAS

Target ORF information:

RefSeq Version	NM_001079812.2
Organism	Homo sapiens(human)
Definition	Homo sapiens diaphanous related formin 1 (DIAPH1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001079812.2

ORF Insert Sequence:

ATGGAGCCGC CCGGCGGGAG CCTGGGGCCC GGCCGCGGGA CCCGGGACAA GAAGAAGGGC 
CGGAGCCCAG ATGAGCTGCC CTCGGCGGGC GGCGACGGCG GCAAATCTAA GAAATTTCTG 
GAGAGATTTA CCAGCATGAG AATTAAGAAG GAGAAGGAAA AGCCCAATTC TGCTCATAGA 
AATTCTTCTG CATCATATGG GGATGATCCC ACAGCACAGT CATTGCAAGA TGTTTCAGAT 
GAACAAGTGC TGGTTCTCTT TGAACAGATG CTGCTGGATA TGAACCTGAA TGAGGAGAAA 
CAGCAACCTT TGAGGGAGAA GGACATCATC ATCAAGAGGG AGATGGTGTC CCAATACTTG 
TACACCTCCA AGGCTGGCAT GAGCCAGAAG GAGAGCTCTA AGTCTGCCAT GATGTATATT 
CAGGAGTTGA GGTCAGGCTT GCGGGATATG CCTCTGCTCA GCTGCCTGGA GTCCCTTCGT 
GTGTCTCTCA ACAACAACCC TGTCAGTTGG GTGCAAACAT TTGGTGCTGA AGGCTTGGCC 
TCCTTATTGG ACATTCTTAA ACGACTTCAT GATGAGAAAG AAGAGACTGC TGGGAGTTAC 
GATAGCCGGA ACAAGCATGA GATCATTCGC TGCTTGAAAG CTTTTATGAA CAACAAGTTT 
GGAATCAAGA CCATGTTGGA GACAGAAGAA GGAATCCTAC TGCTGGTCAG AGCCATGGAT 
CCTGCTGTTC CCAACATGAT GATTGATGCA GCTAAGCTGC TTTCTGCTCT TTGTATTCTA 
CCGCAGCCAG AGGACATGAA TGAAAGGGTT TTGGAGGCAA TGACAGAAAG AGCTGAGATG 
GATGAAGTGG AACGTTTCCA GCCGCTGCTG GATGGATTAA AAAGTGGAAC CACTATTGCA 
CTGAAGGTTG GATGCCTACA GCTGATCAAT GCTCTCATCA CACCAGCGGA GGAACTTGAC 
TTCCGAGTTC ACATCAGAAG TGAACTGATG CGTTTGGGGC TACATCAGGT GTTGCAGGAC 
CTTCGAGAGA TTGAAAATGA AGATATGAGA GTGCAACTAA ATGTGTTTGA TGAACAAGGG 
GAAGAGGATT CCTATGACCT GAAGGGACGG CTGGATGACA TTCGCATGGA GATGGATGAC 
TTTAATGAAG TCTTTCAGAT TCTCTTAAAC ACAGTGAAGG ATTCAAAGGC AGAGCCACAC 
TTCCTTTCCA TCCTGCAGCA CTTACTCTTG GTCCGAAATG ACTATGAGGC CAGACCTCAG 
TACTATAAGT TGATTGAAGA ATGTATTTCC CAGATAGTTC TGCACAAGAA CGGGGCTGAT 
CCTGACTTCA AGTGCCGGCA CCTCCAGATT GAGATTGAGG GATTAATTGA TCAAATGATT 
GATAAGACAA AGGTGGAGAA ATCTGAAGCC AAAGCTGCAG AGCTGGAAAA GAAGTTGGAC 
TCAGAGTTAA CAGCCCGACA TGAGCTACAG GTGGAAATGA AAAAGATGGA AAGTGACTTT 
GAGCAGAAGC TTCAAGATCT TCAGGGAGAA AAAGATGCAC TGCATTCTGA AAAGCAGCAA 
ATTGCCACAG AGAAACAGGA CCTGGAAGCA GAGGTGTCCC AGCTCACAGG AGAGGTTGCC 
AAGCTGACAA AGGAACTGGA AGATGCCAAG AAAGAAATGG CTTCCCTCTC TGCGGCAGCT 
ATTACTGTAC CTCCTTCTGT TCCTAGTCGT GCTCCTGTTC CCCCTGCCCC TCCTTTACCT 
GGTGACTCTG GCACTATTAT TCCACCACCA CCTGCTCCTG GGGATAGTAC CACTCCTCCT 
CCTCCTCCTC CTCCTCCTCC TCCTCCACCT CCTTTGCCTG GGGGTGTTTG CATCTCCTCA 
CCCCCTTCTT TACCTGGAGG TACTGCTATC TCTCCACCCC CTCCTTTGTC TGGGGATGCT 
ACCATCCCTC CACCCCCTCC TTTGCCTGAG GGTGTTGGCA TCCCTTCACC CTCTTCTTTG 
CCTGGAGGTA CTGCCATCCC CCCACCTCCT CCTTTGCCTG GGAGTGCTAG AATCCCCCCA 
CCACCACCTC CTTTGCCTGG GAGTGCTGGA ATTCCCCCCC CACCTCCTCC CTTGCCTGGA 
GAAGCAGGAA TGCCACCTCC TCCTCCCCCT CTTCCTGGTG GTCCTGGAAT CCCTCCACCT 
CCTCCATTTC CCGGAGGCCC TGGCATTCCT CCACCTCCAC CCGGAATGGG TATGCCTCCA 
CCTCCCCCAT TTGGATTTGG AGTTCCTGCA GCCCCAGTTC TGCCATTTGG ATTAACCCCC 
AAAAAGCTTT ATAAGCCAGA GGTGCAGCTC CGGAGGCCAA ACTGGTCCAA GCTTGTGGCT 
GAGGACCTCT CCCAGGACTG CTTCTGGACA AAGGTGAAGG AGGACCGCTT TGAGAACAAT 
GAACTTTTCG CCAAACTTAC CCTTACCTTC TCTGCCCAGA CCAAGACTTC CAAAGCCAAG 
AAGGATCAAG AAGGTGGAGA AGAAAAGAAA TCTGTGCAAA AGAAAAAAGT AAAAGAGTTA 
AAGGTGTTGG ATTCAAAGAC AGCCCAGAAT CTCTCAATCT TTTTGGGTTC CTTCCGCATG 
CCCTATCAAG AGATTAAGAA TGTCATCCTG GAGGTGAATG AGGCTGTTCT GACTGAGTCT 
ATGATCCAGA ACCTCATTAA GCAAATGCCA GAGCCAGAGC AGTTAAAAAT GCTTTCTGAA 
CTGAAGGATG AATATGATGA CCTGGCTGAG TCAGAGCAGT TTGGCGTGGT GATGGGCACT 
GTGCCCCGAC TGCGGCCTCG CCTCAATGCC ATTCTCTTCA AGCTACAATT CAGCGAGCAA 
GTGGAGAATA TCAAGCCAGA GATTGTGTCT GTCACTGCTG CATGTGAGGA GTTACGTAAG 
AGTGAGAGCT TTTCCAATCT CCTAGAGATT ACCTTGCTTG TTGGAAATTA CATGAATGCT 
GGCTCCAGAA ATGCTGGTGC TTTTGGCTTC AATATCAGCT TCCTCTGTAA GCTTCGAGAC 
ACCAAGTCCA CAGATCAGAA GATGACGTTG TTACACTTCT TGGCTGAGTT GTGTGAGAAT 
GACTATCCCG ATGTCCTCAA GTTTCCAGAC GAGCTTGCCC ATGTGGAGAA AGCCAGCCGA 
GTTTCTGCTG AAAACTTGCA AAAGAACCTA GATCAGATGA AGAAACAAAT TTCTGATGTG 
GAACGTGATG TTCAGAATTT CCCAGCTGCC ACAGATGAAA AAGACAAGTT TGTTGAAAAA 
ATGACCAGCT TTGTGAAGGA TGCACAGGAA CAGTATAACA AGCTGCGGAT GATGCATTCT 
AACATGGAGA CCCTCTATAA GGAGCTGGGC GAGTACTTCC TCTTTGACCC CAAGAAGTTG 
TCTGTTGAAG AATTTTTCAT GGATCTTCAC AATTTTCGGA ATATGTTTTT GCAAGCAGTC 
AAGGAGAACC AGAAGCGGCG GGAGACAGAA GAAAAGATGA GGCGAGCAAA ACTAGCCAAG 
GAGAAGGCAG AGAAGGAGCG GCTAGAGAAG CAGCAGAAGA GAGAGCAACT CATAGACATG 
AATGCAGAGG GCGATGAGAC AGGTGTGATG GACAGTCTTC TAGAAGCCCT GCAGTCAGGG 
GCAGCATTCC GACGGAAGAG AGGGCCCCGT CAAGCCAACA GGAAGGCCGG GTGTGCAGTC 
ACATCTCTGC TAGCTTCGGA GCTGACCAAG GATGATGCCA TGGCTGCTGT TCCTGCCAAG 
GTGTCCAAGA ACAGTGAGAC ATTCCCCACA ATCCTTGAGG AAGCCAAGGA GTTGGTTGGC 
CGTGCAAGCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DIAPH1 ( NM_001079812.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001073280.1 Homo sapiens diaphanous related formin 1 (DIAPH1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu26133
Clone ID Related Accession (Same CDS sequence)	NM_001079812.2 , NM_001079812.3
Accession Version	NM_001079812.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3792bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-09
Organism	Homo sapiens(human)
Product	protein diaphanous homolog 1 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA593734.1, BC117257.1, AK023345.1 and AY007129.1. This sequence is a reference standard in the RefSeqGene project. On May 31, 2019 this sequence version replaced NM_001079812.2. Transcript Variant: This variant (2) lacks an in-frame exon in the 5' coding region, as compared to variant 1, which results in a shorter isoform (2). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR7346977.2479246.1, SRR7346977.2276930.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGCCGC CCGGCGGGAG CCTGGGGCCC GGCCGCGGGA CCCGGGACAA GAAGAAGGGC 
CGGAGCCCAG ATGAGCTGCC CTCGGCGGGC GGCGACGGCG GCAAATCTAA GAAATTTCTG 
GAGAGATTTA CCAGCATGAG AATTAAGAAG GAGAAGGAAA AGCCCAATTC TGCTCATAGA 
AATTCTTCTG CATCATATGG GGATGATCCC ACAGCACAGT CATTGCAAGA TGTTTCAGAT 
GAACAAGTGC TGGTTCTCTT TGAACAGATG CTGCTGGATA TGAACCTGAA TGAGGAGAAA 
CAGCAACCTT TGAGGGAGAA GGACATCATC ATCAAGAGGG AGATGGTGTC CCAATACTTG 
TACACCTCCA AGGCTGGCAT GAGCCAGAAG GAGAGCTCTA AGTCTGCCAT GATGTATATT 
CAGGAGTTGA GGTCAGGCTT GCGGGATATG CCTCTGCTCA GCTGCCTGGA GTCCCTTCGT 
GTGTCTCTCA ACAACAACCC TGTCAGTTGG GTGCAAACAT TTGGTGCTGA AGGCTTGGCC 
TCCTTATTGG ACATTCTTAA ACGACTTCAT GATGAGAAAG AAGAGACTGC TGGGAGTTAC 
GATAGCCGGA ACAAGCATGA GATCATTCGC TGCTTGAAAG CTTTTATGAA CAACAAGTTT 
GGAATCAAGA CCATGTTGGA GACAGAAGAA GGAATCCTAC TGCTGGTCAG AGCCATGGAT 
CCTGCTGTTC CCAACATGAT GATTGATGCA GCTAAGCTGC TTTCTGCTCT TTGTATTCTA 
CCGCAGCCAG AGGACATGAA TGAAAGGGTT TTGGAGGCAA TGACAGAAAG AGCTGAGATG 
GATGAAGTGG AACGTTTCCA GCCGCTGCTG GATGGATTAA AAAGTGGAAC CACTATTGCA 
CTGAAGGTTG GATGCCTACA GCTGATCAAT GCTCTCATCA CACCAGCGGA GGAACTTGAC 
TTCCGAGTTC ACATCAGAAG TGAACTGATG CGTTTGGGGC TACATCAGGT GTTGCAGGAC 
CTTCGAGAGA TTGAAAATGA AGATATGAGA GTGCAACTAA ATGTGTTTGA TGAACAAGGG 
GAAGAGGATT CCTATGACCT GAAGGGACGG CTGGATGACA TTCGCATGGA GATGGATGAC 
TTTAATGAAG TCTTTCAGAT TCTCTTAAAC ACAGTGAAGG ATTCAAAGGC AGAGCCACAC 
TTCCTTTCCA TCCTGCAGCA CTTACTCTTG GTCCGAAATG ACTATGAGGC CAGACCTCAG 
TACTATAAGT TGATTGAAGA ATGTATTTCC CAGATAGTTC TGCACAAGAA CGGGGCTGAT 
CCTGACTTCA AGTGCCGGCA CCTCCAGATT GAGATTGAGG GATTAATTGA TCAAATGATT 
GATAAGACAA AGGTGGAGAA ATCTGAAGCC AAAGCTGCAG AGCTGGAAAA GAAGTTGGAC 
TCAGAGTTAA CAGCCCGACA TGAGCTACAG GTGGAAATGA AAAAGATGGA AAGTGACTTT 
GAGCAGAAGC TTCAAGATCT TCAGGGAGAA AAAGATGCAC TGCATTCTGA AAAGCAGCAA 
ATTGCCACAG AGAAACAGGA CCTGGAAGCA GAGGTGTCCC AGCTCACAGG AGAGGTTGCC 
AAGCTGACAA AGGAACTGGA AGATGCCAAG AAAGAAATGG CTTCCCTCTC TGCGGCAGCT 
ATTACTGTAC CTCCTTCTGT TCCTAGTCGT GCTCCTGTTC CCCCTGCCCC TCCTTTACCT 
GGTGACTCTG GCACTATTAT TCCACCACCA CCTGCTCCTG GGGATAGTAC CACTCCTCCT 
CCTCCTCCTC CTCCTCCTCC TCCTCCACCT CCTTTGCCTG GGGGTGTTTG CATCTCCTCA 
CCCCCTTCTT TACCTGGAGG TACTGCTATC TCTCCACCCC CTCCTTTGTC TGGGGATGCT 
ACCATCCCTC CACCCCCTCC TTTGCCTGAG GGTGTTGGCA TCCCTTCACC CTCTTCTTTG 
CCTGGAGGTA CTGCCATCCC CCCACCTCCT CCTTTGCCTG GGAGTGCTAG AATCCCCCCA 
CCACCACCTC CTTTGCCTGG GAGTGCTGGA ATTCCCCCCC CACCTCCTCC CTTGCCTGGA 
GAAGCAGGAA TGCCACCTCC TCCTCCCCCT CTTCCTGGTG GTCCTGGAAT CCCTCCACCT 
CCTCCATTTC CCGGAGGCCC TGGCATTCCT CCACCTCCAC CCGGAATGGG TATGCCTCCA 
CCTCCCCCAT TTGGATTTGG AGTTCCTGCA GCCCCAGTTC TGCCATTTGG ATTAACCCCC 
AAAAAGCTTT ATAAGCCAGA GGTGCAGCTC CGGAGGCCAA ACTGGTCCAA GCTTGTGGCT 
GAGGACCTCT CCCAGGACTG CTTCTGGACA AAGGTGAAGG AGGACCGCTT TGAGAACAAT 
GAACTTTTCG CCAAACTTAC CCTTACCTTC TCTGCCCAGA CCAAGACTTC CAAAGCCAAG 
AAGGATCAAG AAGGTGGAGA AGAAAAGAAA TCTGTGCAAA AGAAAAAAGT AAAAGAGTTA 
AAGGTGTTGG ATTCAAAGAC AGCCCAGAAT CTCTCAATCT TTTTGGGTTC CTTCCGCATG 
CCCTATCAAG AGATTAAGAA TGTCATCCTG GAGGTGAATG AGGCTGTTCT GACTGAGTCT 
ATGATCCAGA ACCTCATTAA GCAAATGCCA GAGCCAGAGC AGTTAAAAAT GCTTTCTGAA 
CTGAAGGATG AATATGATGA CCTGGCTGAG TCAGAGCAGT TTGGCGTGGT GATGGGCACT 
GTGCCCCGAC TGCGGCCTCG CCTCAATGCC ATTCTCTTCA AGCTACAATT CAGCGAGCAA 
GTGGAGAATA TCAAGCCAGA GATTGTGTCT GTCACTGCTG CATGTGAGGA GTTACGTAAG 
AGTGAGAGCT TTTCCAATCT CCTAGAGATT ACCTTGCTTG TTGGAAATTA CATGAATGCT 
GGCTCCAGAA ATGCTGGTGC TTTTGGCTTC AATATCAGCT TCCTCTGTAA GCTTCGAGAC 
ACCAAGTCCA CAGATCAGAA GATGACGTTG TTACACTTCT TGGCTGAGTT GTGTGAGAAT 
GACTATCCCG ATGTCCTCAA GTTTCCAGAC GAGCTTGCCC ATGTGGAGAA AGCCAGCCGA 
GTTTCTGCTG AAAACTTGCA AAAGAACCTA GATCAGATGA AGAAACAAAT TTCTGATGTG 
GAACGTGATG TTCAGAATTT CCCAGCTGCC ACAGATGAAA AAGACAAGTT TGTTGAAAAA 
ATGACCAGCT TTGTGAAGGA TGCACAGGAA CAGTATAACA AGCTGCGGAT GATGCATTCT 
AACATGGAGA CCCTCTATAA GGAGCTGGGC GAGTACTTCC TCTTTGACCC CAAGAAGTTG 
TCTGTTGAAG AATTTTTCAT GGATCTTCAC AATTTTCGGA ATATGTTTTT GCAAGCAGTC 
AAGGAGAACC AGAAGCGGCG GGAGACAGAA GAAAAGATGA GGCGAGCAAA ACTAGCCAAG 
GAGAAGGCAG AGAAGGAGCG GCTAGAGAAG CAGCAGAAGA GAGAGCAACT CATAGACATG 
AATGCAGAGG GCGATGAGAC AGGTGTGATG GACAGTCTTC TAGAAGCCCT GCAGTCAGGG 
GCAGCATTCC GACGGAAGAG AGGGCCCCGT CAAGCCAACA GGAAGGCCGG GTGTGCAGTC 
ACATCTCTGC TAGCTTCGGA GCTGACCAAG GATGATGCCA TGGCTGCTGT TCCTGCCAAG 
GTGTCCAAGA ACAGTGAGAC ATTCCCCACA ATCCTTGAGG AAGCCAAGGA GTTGGTTGGC 
CGTGCAAGCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001073280.1
CDS	87..3878
Translation	MEPPGGSLGPGRGTRDKKKGRSPDELPSAGGDGGKSKKFLERFTSMRIKKEKEKPNSAHRNSSASYGDDPTAQSLQDVSDEQVLVLFEQMLLDMNLNEEKQQPLREKDIIIKREMVSQYLYTSKAGMSQKESSKSAMMYIQELRSGLRDMPLLSCLESLRVSLNNNPVSWVQTFGAEGLASLLDILKRLHDEKEETAGSYDSRNKHEIIRCLKAFMNNKFGIKTMLETEEGILLLVRAMDPAVPNMMIDAAKLLSALCILPQPEDMNERVLEAMTERAEMDEVERFQPLLDGLKSGTTIALKVGCLQLINALITPAEELDFRVHIRSELMRLGLHQVLQDLREIENEDMRVQLNVFDEQGEEDSYDLKGRLDDIRMEMDDFNEVFQILLNTVKDSKAEPHFLSILQHLLLVRNDYEARPQYYKLIEECISQIVLHKNGADPDFKCRHLQIEIEGLIDQMIDKTKVEKSEAKAAELEKKLDSELTARHELQVEMKKMESDFEQKLQDLQGEKDALHSEKQQIATEKQDLEAEVSQLTGEVAKLTKELEDAKKEMASLSAAAITVPPSVPSRAPVPPAPPLPGDSGTIIPPPPAPGDSTTPPPPPPPPPPPPPLPGGVCISSPPSLPGGTAISPPPPLSGDATIPPPPPLPEGVGIPSPSSLPGGTAIPPPPPLPGSARIPPPPPPLPGSAGIPPPPPPLPGEAGMPPPPPPLPGGPGIPPPPPFPGGPGIPPPPPGMGMPPPPPFGFGVPAAPVLPFGLTPKKLYKPEVQLRRPNWSKLVAEDLSQDCFWTKVKEDRFENNELFAKLTLTFSAQTKTSKAKKDQEGGEEKKSVQKKKVKELKVLDSKTAQNLSIFLGSFRMPYQEIKNVILEVNEAVLTESMIQNLIKQMPEPEQLKMLSELKDEYDDLAESEQFGVVMGTVPRLRPRLNAILFKLQFSEQVENIKPEIVSVTAACEELRKSESFSNLLEITLLVGNYMNAGSRNAGAFGFNISFLCKLRDTKSTDQKMTLLHFLAELCENDYPDVLKFPDELAHVEKASRVSAENLQKNLDQMKKQISDVERDVQNFPAATDEKDKFVEKMTSFVKDAQEQYNKLRMMHSNMETLYKELGEYFLFDPKKLSVEEFFMDLHNFRNMFLQAVKENQKRRETEEKMRRAKLAKEKAEKERLEKQQKREQLIDMNAEGDETGVMDSLLEALQSGAAFRRKRGPRQANRKAGCAVTSLLASELTKDDAMAAVPAKVSKNSETFPTILEEAKELVGRAS

Target ORF information:

RefSeq Version	NM_001079812.3
Organism	Homo sapiens(human)
Definition	Homo sapiens diaphanous related formin 1 (DIAPH1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001079812.3

ORF Insert Sequence:

ATGGAGCCGC CCGGCGGGAG CCTGGGGCCC GGCCGCGGGA CCCGGGACAA GAAGAAGGGC 
CGGAGCCCAG ATGAGCTGCC CTCGGCGGGC GGCGACGGCG GCAAATCTAA GAAATTTCTG 
GAGAGATTTA CCAGCATGAG AATTAAGAAG GAGAAGGAAA AGCCCAATTC TGCTCATAGA 
AATTCTTCTG CATCATATGG GGATGATCCC ACAGCACAGT CATTGCAAGA TGTTTCAGAT 
GAACAAGTGC TGGTTCTCTT TGAACAGATG CTGCTGGATA TGAACCTGAA TGAGGAGAAA 
CAGCAACCTT TGAGGGAGAA GGACATCATC ATCAAGAGGG AGATGGTGTC CCAATACTTG 
TACACCTCCA AGGCTGGCAT GAGCCAGAAG GAGAGCTCTA AGTCTGCCAT GATGTATATT 
CAGGAGTTGA GGTCAGGCTT GCGGGATATG CCTCTGCTCA GCTGCCTGGA GTCCCTTCGT 
GTGTCTCTCA ACAACAACCC TGTCAGTTGG GTGCAAACAT TTGGTGCTGA AGGCTTGGCC 
TCCTTATTGG ACATTCTTAA ACGACTTCAT GATGAGAAAG AAGAGACTGC TGGGAGTTAC 
GATAGCCGGA ACAAGCATGA GATCATTCGC TGCTTGAAAG CTTTTATGAA CAACAAGTTT 
GGAATCAAGA CCATGTTGGA GACAGAAGAA GGAATCCTAC TGCTGGTCAG AGCCATGGAT 
CCTGCTGTTC CCAACATGAT GATTGATGCA GCTAAGCTGC TTTCTGCTCT TTGTATTCTA 
CCGCAGCCAG AGGACATGAA TGAAAGGGTT TTGGAGGCAA TGACAGAAAG AGCTGAGATG 
GATGAAGTGG AACGTTTCCA GCCGCTGCTG GATGGATTAA AAAGTGGAAC CACTATTGCA 
CTGAAGGTTG GATGCCTACA GCTGATCAAT GCTCTCATCA CACCAGCGGA GGAACTTGAC 
TTCCGAGTTC ACATCAGAAG TGAACTGATG CGTTTGGGGC TACATCAGGT GTTGCAGGAC 
CTTCGAGAGA TTGAAAATGA AGATATGAGA GTGCAACTAA ATGTGTTTGA TGAACAAGGG 
GAAGAGGATT CCTATGACCT GAAGGGACGG CTGGATGACA TTCGCATGGA GATGGATGAC 
TTTAATGAAG TCTTTCAGAT TCTCTTAAAC ACAGTGAAGG ATTCAAAGGC AGAGCCACAC 
TTCCTTTCCA TCCTGCAGCA CTTACTCTTG GTCCGAAATG ACTATGAGGC CAGACCTCAG 
TACTATAAGT TGATTGAAGA ATGTATTTCC CAGATAGTTC TGCACAAGAA CGGGGCTGAT 
CCTGACTTCA AGTGCCGGCA CCTCCAGATT GAGATTGAGG GATTAATTGA TCAAATGATT 
GATAAGACAA AGGTGGAGAA ATCTGAAGCC AAAGCTGCAG AGCTGGAAAA GAAGTTGGAC 
TCAGAGTTAA CAGCCCGACA TGAGCTACAG GTGGAAATGA AAAAGATGGA AAGTGACTTT 
GAGCAGAAGC TTCAAGATCT TCAGGGAGAA AAAGATGCAC TGCATTCTGA AAAGCAGCAA 
ATTGCCACAG AGAAACAGGA CCTGGAAGCA GAGGTGTCCC AGCTCACAGG AGAGGTTGCC 
AAGCTGACAA AGGAACTGGA AGATGCCAAG AAAGAAATGG CTTCCCTCTC TGCGGCAGCT 
ATTACTGTAC CTCCTTCTGT TCCTAGTCGT GCTCCTGTTC CCCCTGCCCC TCCTTTACCT 
GGTGACTCTG GCACTATTAT TCCACCACCA CCTGCTCCTG GGGATAGTAC CACTCCTCCT 
CCTCCTCCTC CTCCTCCTCC TCCTCCACCT CCTTTGCCTG GGGGTGTTTG CATCTCCTCA 
CCCCCTTCTT TACCTGGAGG TACTGCTATC TCTCCACCCC CTCCTTTGTC TGGGGATGCT 
ACCATCCCTC CACCCCCTCC TTTGCCTGAG GGTGTTGGCA TCCCTTCACC CTCTTCTTTG 
CCTGGAGGTA CTGCCATCCC CCCACCTCCT CCTTTGCCTG GGAGTGCTAG AATCCCCCCA 
CCACCACCTC CTTTGCCTGG GAGTGCTGGA ATTCCCCCCC CACCTCCTCC CTTGCCTGGA 
GAAGCAGGAA TGCCACCTCC TCCTCCCCCT CTTCCTGGTG GTCCTGGAAT CCCTCCACCT 
CCTCCATTTC CCGGAGGCCC TGGCATTCCT CCACCTCCAC CCGGAATGGG TATGCCTCCA 
CCTCCCCCAT TTGGATTTGG AGTTCCTGCA GCCCCAGTTC TGCCATTTGG ATTAACCCCC 
AAAAAGCTTT ATAAGCCAGA GGTGCAGCTC CGGAGGCCAA ACTGGTCCAA GCTTGTGGCT 
GAGGACCTCT CCCAGGACTG CTTCTGGACA AAGGTGAAGG AGGACCGCTT TGAGAACAAT 
GAACTTTTCG CCAAACTTAC CCTTACCTTC TCTGCCCAGA CCAAGACTTC CAAAGCCAAG 
AAGGATCAAG AAGGTGGAGA AGAAAAGAAA TCTGTGCAAA AGAAAAAAGT AAAAGAGTTA 
AAGGTGTTGG ATTCAAAGAC AGCCCAGAAT CTCTCAATCT TTTTGGGTTC CTTCCGCATG 
CCCTATCAAG AGATTAAGAA TGTCATCCTG GAGGTGAATG AGGCTGTTCT GACTGAGTCT 
ATGATCCAGA ACCTCATTAA GCAAATGCCA GAGCCAGAGC AGTTAAAAAT GCTTTCTGAA 
CTGAAGGATG AATATGATGA CCTGGCTGAG TCAGAGCAGT TTGGCGTGGT GATGGGCACT 
GTGCCCCGAC TGCGGCCTCG CCTCAATGCC ATTCTCTTCA AGCTACAATT CAGCGAGCAA 
GTGGAGAATA TCAAGCCAGA GATTGTGTCT GTCACTGCTG CATGTGAGGA GTTACGTAAG 
AGTGAGAGCT TTTCCAATCT CCTAGAGATT ACCTTGCTTG TTGGAAATTA CATGAATGCT 
GGCTCCAGAA ATGCTGGTGC TTTTGGCTTC AATATCAGCT TCCTCTGTAA GCTTCGAGAC 
ACCAAGTCCA CAGATCAGAA GATGACGTTG TTACACTTCT TGGCTGAGTT GTGTGAGAAT 
GACTATCCCG ATGTCCTCAA GTTTCCAGAC GAGCTTGCCC ATGTGGAGAA AGCCAGCCGA 
GTTTCTGCTG AAAACTTGCA AAAGAACCTA GATCAGATGA AGAAACAAAT TTCTGATGTG 
GAACGTGATG TTCAGAATTT CCCAGCTGCC ACAGATGAAA AAGACAAGTT TGTTGAAAAA 
ATGACCAGCT TTGTGAAGGA TGCACAGGAA CAGTATAACA AGCTGCGGAT GATGCATTCT 
AACATGGAGA CCCTCTATAA GGAGCTGGGC GAGTACTTCC TCTTTGACCC CAAGAAGTTG 
TCTGTTGAAG AATTTTTCAT GGATCTTCAC AATTTTCGGA ATATGTTTTT GCAAGCAGTC 
AAGGAGAACC AGAAGCGGCG GGAGACAGAA GAAAAGATGA GGCGAGCAAA ACTAGCCAAG 
GAGAAGGCAG AGAAGGAGCG GCTAGAGAAG CAGCAGAAGA GAGAGCAACT CATAGACATG 
AATGCAGAGG GCGATGAGAC AGGTGTGATG GACAGTCTTC TAGAAGCCCT GCAGTCAGGG 
GCAGCATTCC GACGGAAGAG AGGGCCCCGT CAAGCCAACA GGAAGGCCGG GTGTGCAGTC 
ACATCTCTGC TAGCTTCGGA GCTGACCAAG GATGATGCCA TGGCTGCTGT TCCTGCCAAG 
GTGTCCAAGA ACAGTGAGAC ATTCCCCACA ATCCTTGAGG AAGCCAAGGA GTTGGTTGGC 
CGTGCAAGCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DIAPH1 ( NM_001314007.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001300936.1 Homo sapiens diaphanous related formin 1 (DIAPH1), transcript variant 3, mRNA.

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CloneID	OHu39824
Clone ID Related Accession (Same CDS sequence)	NM_001314007.1 , NM_001314007.2
Accession Version	NM_001314007.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3753bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-25
Organism	Homo sapiens(human)
Product	protein diaphanous homolog 1 isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA593734.1, BC143414.1, AC008781.7 and AY007129.1. On Sep 23, 2015 this sequence version replaced XM_005268384.2. Transcript Variant: This variant (3) contains an alternate exon in the 3' end compared to variant 1, that causes a frameshift. The resulting isoform (3) has a shorter and distinct C-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC143414.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGCCGC CCGGCGGGAG CCTGGGGCCC GGCCGCGGGA CCCGGGACAA GAAGAAGGGC 
CGGAGCCCAG ATGAGCTGCC CTCGGCGGGC GGCGACGGCG GCAAATCTAA GAAATTTACT 
CTGAAGCGGC TCATGGCAGA TGAGCTGGAG AGATTTACCA GCATGAGAAT TAAGAAGGAG 
AAGGAAAAGC CCAATTCTGC TCATAGAAAT TCTTCTGCAT CATATGGGGA TGATCCCACA 
GCACAGTCAT TGCAAGATGT TTCAGATGAA CAAGTGCTGG TTCTCTTTGA ACAGATGCTG 
CTGGATATGA ACCTGAATGA GGAGAAACAG CAACCTTTGA GGGAGAAGGA CATCATCATC 
AAGAGGGAGA TGGTGTCCCA ATACTTGTAC ACCTCCAAGG CTGGCATGAG CCAGAAGGAG 
AGCTCTAAGT CTGCCATGAT GTATATTCAG GAGTTGAGGT CAGGCTTGCG GGATATGCCT 
CTGCTCAGCT GCCTGGAGTC CCTTCGTGTG TCTCTCAACA ACAACCCTGT CAGTTGGGTG 
CAAACATTTG GTGCTGAAGG CTTGGCCTCC TTATTGGACA TTCTTAAACG ACTTCATGAT 
GAGAAAGAAG AGACTGCTGG GAGTTACGAT AGCCGGAACA AGCATGAGAT CATTCGCTGC 
TTGAAAGCTT TTATGAACAA CAAGTTTGGA ATCAAGACCA TGTTGGAGAC AGAAGAAGGA 
ATCCTACTGC TGGTCAGAGC CATGGATCCT GCTGTTCCCA ACATGATGAT TGATGCAGCT 
AAGCTGCTTT CTGCTCTTTG TATTCTACCG CAGCCAGAGG ACATGAATGA AAGGGTTTTG 
GAGGCAATGA CAGAAAGAGC TGAGATGGAT GAAGTGGAAC GTTTCCAGCC GCTGCTGGAT 
GGATTAAAAA GTGGAACCAC TATTGCACTG AAGGTTGGAT GCCTACAGCT GATCAATGCT 
CTCATCACAC CAGCGGAGGA ACTTGACTTC CGAGTTCACA TCAGAAGTGA ACTGATGCGT 
TTGGGGCTAC ATCAGGTGTT GCAGGACCTT CGAGAGATTG AAAATGAAGA TATGAGAGTG 
CAACTAAATG TGTTTGATGA ACAAGGGGAA GAGGATTCCT ATGACCTGAA GGGACGGCTG 
GATGACATTC GCATGGAGAT GGATGACTTT AATGAAGTCT TTCAGATTCT CTTAAACACA 
GTGAAGGATT CAAAGGCAGA GCCACACTTC CTTTCCATCC TGCAGCACTT ACTCTTGGTC 
CGAAATGACT ATGAGGCCAG ACCTCAGTAC TATAAGTTGA TTGAAGAATG TATTTCCCAG 
ATAGTTCTGC ACAAGAACGG GGCTGATCCT GACTTCAAGT GCCGGCACCT CCAGATTGAG 
ATTGAGGGAT TAATTGATCA AATGATTGAT AAGACAAAGG TGGAGAAATC TGAAGCCAAA 
GCTGCAGAGC TGGAAAAGAA GTTGGACTCA GAGTTAACAG CCCGACATGA GCTACAGGTG 
GAAATGAAAA AGATGGAAAG TGACTTTGAG CAGAAGCTTC AAGATCTTCA GGGAGAAAAA 
GATGCACTGC ATTCTGAAAA GCAGCAAATT GCCACAGAGA AACAGGACCT GGAAGCAGAG 
GTGTCCCAGC TCACAGGAGA GGTTGCCAAG CTGACAAAGG AACTGGAAGA TGCCAAGAAA 
GAAATGGCTT CCCTCTCTGC GGCAGCTATT ACTGTACCTC CTTCTGTTCC TAGTCGTGCT 
CCTGTTCCCC CTGCCCCTCC TTTACCTGGT GACTCTGGCA CTATTATTCC ACCACCACCT 
GCTCCTGGGG ATAGTACCAC TCCTCCTCCT CCTCCTCCTC CTCCTCCTCC TCCACCTCCT 
TTGCCTGGGG GTGTTTGCAT CTCCTCACCC CCTTCTTTAC CTGGAGGTAC TGCTATCTCT 
CCACCCCCTC CTTTGTCTGG GGATGCTACC ATCCCTCCAC CCCCTCCTTT GCCTGAGGGT 
GTTGGCATCC CTTCACCCTC TTCTTTGCCT GGAGGTACTG CCATCCCCCC ACCTCCTCCT 
TTGCCTGGGA GTGCTAGAAT CCCCCCACCA CCACCTCCTT TGCCTGGGAG TGCTGGAATT 
CCCCCCCCAC CTCCTCCCTT GCCTGGAGAA GCAGGAATGC CACCTCCTCC TCCCCCTCTT 
CCTGGTGGTC CTGGAATCCC TCCACCTCCT CCATTTCCCG GAGGCCCTGG CATTCCTCCA 
CCTCCACCCG GAATGGGTAT GCCTCCACCT CCCCCATTTG GATTTGGAGT TCCTGCAGCC 
CCAGTTCTGC CATTTGGATT AACCCCCAAA AAGCTTTATA AGCCAGAGGT GCAGCTCCGG 
AGGCCAAACT GGTCCAAGCT TGTGGCTGAG GACCTCTCCC AGGACTGCTT CTGGACAAAG 
GTGAAGGAGG ACCGCTTTGA GAACAATGAA CTTTTCGCCA AACTTACCCT TACCTTCTCT 
GCCCAGACCA AGACTTCCAA AGCCAAGAAG GATCAAGAAG GTGGAGAAGA AAAGAAATCT 
GTGCAAAAGA AAAAAGTAAA AGAGTTAAAG GTGTTGGATT CAAAGACAGC CCAGAATCTC 
TCAATCTTTT TGGGTTCCTT CCGCATGCCC TATCAAGAGA TTAAGAATGT CATCCTGGAG 
GTGAATGAGG CTGTTCTGAC TGAGTCTATG ATCCAGAACC TCATTAAGCA AATGCCAGAG 
CCAGAGCAGT TAAAAATGCT TTCTGAACTG AAGGATGAAT ATGATGACCT GGCTGAGTCA 
GAGCAGTTTG GCGTGGTGAT GGGCACTGTG CCCCGACTGC GGCCTCGCCT CAATGCCATT 
CTCTTCAAGC TACAATTCAG CGAGCAAGTG GAGAATATCA AGCCAGAGAT TGTGTCTGTC 
ACTGCTGCAT GTGAGGAGTT ACGTAAGAGT GAGAGCTTTT CCAATCTCCT AGAGATTACC 
TTGCTTGTTG GAAATTACAT GAATGCTGGC TCCAGAAATG CTGGTGCTTT TGGCTTCAAT 
ATCAGCTTCC TCTGTAAGCT TCGAGACACC AAGTCCACAG ATCAGAAGAT GACGTTGTTA 
CACTTCTTGG CTGAGTTGTG TGAGAATGAC TATCCCGATG TCCTCAAGTT TCCAGACGAG 
CTTGCCCATG TGGAGAAAGC CAGCCGAGTT TCTGCTGAAA ACTTGCAAAA GAACCTAGAT 
CAGATGAAGA AACAAATTTC TGATGTGGAA CGTGATGTTC AGAATTTCCC AGCTGCCACA 
GATGAAAAAG ACAAGTTTGT TGAAAAAATG ACCAGCTTTG TGAAGGATGC ACAGGAACAG 
TATAACAAGC TGCGGATGAT GCATTCTAAC ATGGAGACCC TCTATAAGGA GCTGGGCGAG 
TACTTCCTCT TTGACCCCAA GAAGTTGTCT GTTGAAGAAT TTTTCATGGA TCTTCACAAT 
TTTCGGAATA TGTTTTTGCA AGCAGTCAAG GAGAACCAGA AGCGGCGGGA GACAGAAGAA 
AAGATGAGGC GAGCAAAACT AGCCAAGGAG AAGGCAGAGA AGGAGCGGCT AGAGAAGCAG 
CAGAAGAGAG AGCAACTCAT AGACATGAAT GCAGAGGGCG ATGAGACAGG TGTGATGGAC 
AGTCTTCTAG AAGCCCTGCA GTCAGGGGCA GCATTCCGAC GGAAGAGAGG GCCCCGTCAA 
GACAACCTCT TGTGCCCCTG GGAAGTAGAG GAGGAGGAGG AGACCTTTAA ATTGCCAACA 
GGAAGGCCGG GTGTGCAGTC ACATCTCTGC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001300936.1
CDS	142..3894
Translation	MEPPGGSLGPGRGTRDKKKGRSPDELPSAGGDGGKSKKFTLKRLMADELERFTSMRIKKEKEKPNSAHRNSSASYGDDPTAQSLQDVSDEQVLVLFEQMLLDMNLNEEKQQPLREKDIIIKREMVSQYLYTSKAGMSQKESSKSAMMYIQELRSGLRDMPLLSCLESLRVSLNNNPVSWVQTFGAEGLASLLDILKRLHDEKEETAGSYDSRNKHEIIRCLKAFMNNKFGIKTMLETEEGILLLVRAMDPAVPNMMIDAAKLLSALCILPQPEDMNERVLEAMTERAEMDEVERFQPLLDGLKSGTTIALKVGCLQLINALITPAEELDFRVHIRSELMRLGLHQVLQDLREIENEDMRVQLNVFDEQGEEDSYDLKGRLDDIRMEMDDFNEVFQILLNTVKDSKAEPHFLSILQHLLLVRNDYEARPQYYKLIEECISQIVLHKNGADPDFKCRHLQIEIEGLIDQMIDKTKVEKSEAKAAELEKKLDSELTARHELQVEMKKMESDFEQKLQDLQGEKDALHSEKQQIATEKQDLEAEVSQLTGEVAKLTKELEDAKKEMASLSAAAITVPPSVPSRAPVPPAPPLPGDSGTIIPPPPAPGDSTTPPPPPPPPPPPPPLPGGVCISSPPSLPGGTAISPPPPLSGDATIPPPPPLPEGVGIPSPSSLPGGTAIPPPPPLPGSARIPPPPPPLPGSAGIPPPPPPLPGEAGMPPPPPPLPGGPGIPPPPPFPGGPGIPPPPPGMGMPPPPPFGFGVPAAPVLPFGLTPKKLYKPEVQLRRPNWSKLVAEDLSQDCFWTKVKEDRFENNELFAKLTLTFSAQTKTSKAKKDQEGGEEKKSVQKKKVKELKVLDSKTAQNLSIFLGSFRMPYQEIKNVILEVNEAVLTESMIQNLIKQMPEPEQLKMLSELKDEYDDLAESEQFGVVMGTVPRLRPRLNAILFKLQFSEQVENIKPEIVSVTAACEELRKSESFSNLLEITLLVGNYMNAGSRNAGAFGFNISFLCKLRDTKSTDQKMTLLHFLAELCENDYPDVLKFPDELAHVEKASRVSAENLQKNLDQMKKQISDVERDVQNFPAATDEKDKFVEKMTSFVKDAQEQYNKLRMMHSNMETLYKELGEYFLFDPKKLSVEEFFMDLHNFRNMFLQAVKENQKRRETEEKMRRAKLAKEKAEKERLEKQQKREQLIDMNAEGDETGVMDSLLEALQSGAAFRRKRGPRQDNLLCPWEVEEEEETFKLPTGRPGVQSHLC

Target ORF information:

RefSeq Version	NM_001314007.1
Organism	Homo sapiens(human)
Definition	Homo sapiens diaphanous related formin 1 (DIAPH1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001314007.1

ORF Insert Sequence:

ATGGAGCCGC CCGGCGGGAG CCTGGGGCCC GGCCGCGGGA CCCGGGACAA GAAGAAGGGC 
CGGAGCCCAG ATGAGCTGCC CTCGGCGGGC GGCGACGGCG GCAAATCTAA GAAATTTACT 
CTGAAGCGGC TCATGGCAGA TGAGCTGGAG AGATTTACCA GCATGAGAAT TAAGAAGGAG 
AAGGAAAAGC CCAATTCTGC TCATAGAAAT TCTTCTGCAT CATATGGGGA TGATCCCACA 
GCACAGTCAT TGCAAGATGT TTCAGATGAA CAAGTGCTGG TTCTCTTTGA ACAGATGCTG 
CTGGATATGA ACCTGAATGA GGAGAAACAG CAACCTTTGA GGGAGAAGGA CATCATCATC 
AAGAGGGAGA TGGTGTCCCA ATACTTGTAC ACCTCCAAGG CTGGCATGAG CCAGAAGGAG 
AGCTCTAAGT CTGCCATGAT GTATATTCAG GAGTTGAGGT CAGGCTTGCG GGATATGCCT 
CTGCTCAGCT GCCTGGAGTC CCTTCGTGTG TCTCTCAACA ACAACCCTGT CAGTTGGGTG 
CAAACATTTG GTGCTGAAGG CTTGGCCTCC TTATTGGACA TTCTTAAACG ACTTCATGAT 
GAGAAAGAAG AGACTGCTGG GAGTTACGAT AGCCGGAACA AGCATGAGAT CATTCGCTGC 
TTGAAAGCTT TTATGAACAA CAAGTTTGGA ATCAAGACCA TGTTGGAGAC AGAAGAAGGA 
ATCCTACTGC TGGTCAGAGC CATGGATCCT GCTGTTCCCA ACATGATGAT TGATGCAGCT 
AAGCTGCTTT CTGCTCTTTG TATTCTACCG CAGCCAGAGG ACATGAATGA AAGGGTTTTG 
GAGGCAATGA CAGAAAGAGC TGAGATGGAT GAAGTGGAAC GTTTCCAGCC GCTGCTGGAT 
GGATTAAAAA GTGGAACCAC TATTGCACTG AAGGTTGGAT GCCTACAGCT GATCAATGCT 
CTCATCACAC CAGCGGAGGA ACTTGACTTC CGAGTTCACA TCAGAAGTGA ACTGATGCGT 
TTGGGGCTAC ATCAGGTGTT GCAGGACCTT CGAGAGATTG AAAATGAAGA TATGAGAGTG 
CAACTAAATG TGTTTGATGA ACAAGGGGAA GAGGATTCCT ATGACCTGAA GGGACGGCTG 
GATGACATTC GCATGGAGAT GGATGACTTT AATGAAGTCT TTCAGATTCT CTTAAACACA 
GTGAAGGATT CAAAGGCAGA GCCACACTTC CTTTCCATCC TGCAGCACTT ACTCTTGGTC 
CGAAATGACT ATGAGGCCAG ACCTCAGTAC TATAAGTTGA TTGAAGAATG TATTTCCCAG 
ATAGTTCTGC ACAAGAACGG GGCTGATCCT GACTTCAAGT GCCGGCACCT CCAGATTGAG 
ATTGAGGGAT TAATTGATCA AATGATTGAT AAGACAAAGG TGGAGAAATC TGAAGCCAAA 
GCTGCAGAGC TGGAAAAGAA GTTGGACTCA GAGTTAACAG CCCGACATGA GCTACAGGTG 
GAAATGAAAA AGATGGAAAG TGACTTTGAG CAGAAGCTTC AAGATCTTCA GGGAGAAAAA 
GATGCACTGC ATTCTGAAAA GCAGCAAATT GCCACAGAGA AACAGGACCT GGAAGCAGAG 
GTGTCCCAGC TCACAGGAGA GGTTGCCAAG CTGACAAAGG AACTGGAAGA TGCCAAGAAA 
GAAATGGCTT CCCTCTCTGC GGCAGCTATT ACTGTACCTC CTTCTGTTCC TAGTCGTGCT 
CCTGTTCCCC CTGCCCCTCC TTTACCTGGT GACTCTGGCA CTATTATTCC ACCACCACCT 
GCTCCTGGGG ATAGTACCAC TCCTCCTCCT CCTCCTCCTC CTCCTCCTCC TCCACCTCCT 
TTGCCTGGGG GTGTTTGCAT CTCCTCACCC CCTTCTTTAC CTGGAGGTAC TGCTATCTCT 
CCACCCCCTC CTTTGTCTGG GGATGCTACC ATCCCTCCAC CCCCTCCTTT GCCTGAGGGT 
GTTGGCATCC CTTCACCCTC TTCTTTGCCT GGAGGTACTG CCATCCCCCC ACCTCCTCCT 
TTGCCTGGGA GTGCTAGAAT CCCCCCACCA CCACCTCCTT TGCCTGGGAG TGCTGGAATT 
CCCCCCCCAC CTCCTCCCTT GCCTGGAGAA GCAGGAATGC CACCTCCTCC TCCCCCTCTT 
CCTGGTGGTC CTGGAATCCC TCCACCTCCT CCATTTCCCG GAGGCCCTGG CATTCCTCCA 
CCTCCACCCG GAATGGGTAT GCCTCCACCT CCCCCATTTG GATTTGGAGT TCCTGCAGCC 
CCAGTTCTGC CATTTGGATT AACCCCCAAA AAGCTTTATA AGCCAGAGGT GCAGCTCCGG 
AGGCCAAACT GGTCCAAGCT TGTGGCTGAG GACCTCTCCC AGGACTGCTT CTGGACAAAG 
GTGAAGGAGG ACCGCTTTGA GAACAATGAA CTTTTCGCCA AACTTACCCT TACCTTCTCT 
GCCCAGACCA AGACTTCCAA AGCCAAGAAG GATCAAGAAG GTGGAGAAGA AAAGAAATCT 
GTGCAAAAGA AAAAAGTAAA AGAGTTAAAG GTGTTGGATT CAAAGACAGC CCAGAATCTC 
TCAATCTTTT TGGGTTCCTT CCGCATGCCC TATCAAGAGA TTAAGAATGT CATCCTGGAG 
GTGAATGAGG CTGTTCTGAC TGAGTCTATG ATCCAGAACC TCATTAAGCA AATGCCAGAG 
CCAGAGCAGT TAAAAATGCT TTCTGAACTG AAGGATGAAT ATGATGACCT GGCTGAGTCA 
GAGCAGTTTG GCGTGGTGAT GGGCACTGTG CCCCGACTGC GGCCTCGCCT CAATGCCATT 
CTCTTCAAGC TACAATTCAG CGAGCAAGTG GAGAATATCA AGCCAGAGAT TGTGTCTGTC 
ACTGCTGCAT GTGAGGAGTT ACGTAAGAGT GAGAGCTTTT CCAATCTCCT AGAGATTACC 
TTGCTTGTTG GAAATTACAT GAATGCTGGC TCCAGAAATG CTGGTGCTTT TGGCTTCAAT 
ATCAGCTTCC TCTGTAAGCT TCGAGACACC AAGTCCACAG ATCAGAAGAT GACGTTGTTA 
CACTTCTTGG CTGAGTTGTG TGAGAATGAC TATCCCGATG TCCTCAAGTT TCCAGACGAG 
CTTGCCCATG TGGAGAAAGC CAGCCGAGTT TCTGCTGAAA ACTTGCAAAA GAACCTAGAT 
CAGATGAAGA AACAAATTTC TGATGTGGAA CGTGATGTTC AGAATTTCCC AGCTGCCACA 
GATGAAAAAG ACAAGTTTGT TGAAAAAATG ACCAGCTTTG TGAAGGATGC ACAGGAACAG 
TATAACAAGC TGCGGATGAT GCATTCTAAC ATGGAGACCC TCTATAAGGA GCTGGGCGAG 
TACTTCCTCT TTGACCCCAA GAAGTTGTCT GTTGAAGAAT TTTTCATGGA TCTTCACAAT 
TTTCGGAATA TGTTTTTGCA AGCAGTCAAG GAGAACCAGA AGCGGCGGGA GACAGAAGAA 
AAGATGAGGC GAGCAAAACT AGCCAAGGAG AAGGCAGAGA AGGAGCGGCT AGAGAAGCAG 
CAGAAGAGAG AGCAACTCAT AGACATGAAT GCAGAGGGCG ATGAGACAGG TGTGATGGAC 
AGTCTTCTAG AAGCCCTGCA GTCAGGGGCA GCATTCCGAC GGAAGAGAGG GCCCCGTCAA 
GACAACCTCT TGTGCCCCTG GGAAGTAGAG GAGGAGGAGG AGACCTTTAA ATTGCCAACA 
GGAAGGCCGG GTGTGCAGTC ACATCTCTGC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DIAPH1 ( NM_001314007.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001300936.1 Homo sapiens diaphanous related formin 1 (DIAPH1), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu39824
Clone ID Related Accession (Same CDS sequence)	NM_001314007.1 , NM_001314007.2
Accession Version	NM_001314007.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3753bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-26
Organism	Homo sapiens(human)
Product	protein diaphanous homolog 1 isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA593734.1, BC143414.1, AC008781.7 and AY007129.1. On May 31, 2019 this sequence version replaced NM_001314007.1. Transcript Variant: This variant (3) contains an alternate exon in the 3' end compared to variant 1, that causes a frameshift. The resulting isoform (3) has a shorter and distinct C-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC143414.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGCCGC CCGGCGGGAG CCTGGGGCCC GGCCGCGGGA CCCGGGACAA GAAGAAGGGC 
CGGAGCCCAG ATGAGCTGCC CTCGGCGGGC GGCGACGGCG GCAAATCTAA GAAATTTACT 
CTGAAGCGGC TCATGGCAGA TGAGCTGGAG AGATTTACCA GCATGAGAAT TAAGAAGGAG 
AAGGAAAAGC CCAATTCTGC TCATAGAAAT TCTTCTGCAT CATATGGGGA TGATCCCACA 
GCACAGTCAT TGCAAGATGT TTCAGATGAA CAAGTGCTGG TTCTCTTTGA ACAGATGCTG 
CTGGATATGA ACCTGAATGA GGAGAAACAG CAACCTTTGA GGGAGAAGGA CATCATCATC 
AAGAGGGAGA TGGTGTCCCA ATACTTGTAC ACCTCCAAGG CTGGCATGAG CCAGAAGGAG 
AGCTCTAAGT CTGCCATGAT GTATATTCAG GAGTTGAGGT CAGGCTTGCG GGATATGCCT 
CTGCTCAGCT GCCTGGAGTC CCTTCGTGTG TCTCTCAACA ACAACCCTGT CAGTTGGGTG 
CAAACATTTG GTGCTGAAGG CTTGGCCTCC TTATTGGACA TTCTTAAACG ACTTCATGAT 
GAGAAAGAAG AGACTGCTGG GAGTTACGAT AGCCGGAACA AGCATGAGAT CATTCGCTGC 
TTGAAAGCTT TTATGAACAA CAAGTTTGGA ATCAAGACCA TGTTGGAGAC AGAAGAAGGA 
ATCCTACTGC TGGTCAGAGC CATGGATCCT GCTGTTCCCA ACATGATGAT TGATGCAGCT 
AAGCTGCTTT CTGCTCTTTG TATTCTACCG CAGCCAGAGG ACATGAATGA AAGGGTTTTG 
GAGGCAATGA CAGAAAGAGC TGAGATGGAT GAAGTGGAAC GTTTCCAGCC GCTGCTGGAT 
GGATTAAAAA GTGGAACCAC TATTGCACTG AAGGTTGGAT GCCTACAGCT GATCAATGCT 
CTCATCACAC CAGCGGAGGA ACTTGACTTC CGAGTTCACA TCAGAAGTGA ACTGATGCGT 
TTGGGGCTAC ATCAGGTGTT GCAGGACCTT CGAGAGATTG AAAATGAAGA TATGAGAGTG 
CAACTAAATG TGTTTGATGA ACAAGGGGAA GAGGATTCCT ATGACCTGAA GGGACGGCTG 
GATGACATTC GCATGGAGAT GGATGACTTT AATGAAGTCT TTCAGATTCT CTTAAACACA 
GTGAAGGATT CAAAGGCAGA GCCACACTTC CTTTCCATCC TGCAGCACTT ACTCTTGGTC 
CGAAATGACT ATGAGGCCAG ACCTCAGTAC TATAAGTTGA TTGAAGAATG TATTTCCCAG 
ATAGTTCTGC ACAAGAACGG GGCTGATCCT GACTTCAAGT GCCGGCACCT CCAGATTGAG 
ATTGAGGGAT TAATTGATCA AATGATTGAT AAGACAAAGG TGGAGAAATC TGAAGCCAAA 
GCTGCAGAGC TGGAAAAGAA GTTGGACTCA GAGTTAACAG CCCGACATGA GCTACAGGTG 
GAAATGAAAA AGATGGAAAG TGACTTTGAG CAGAAGCTTC AAGATCTTCA GGGAGAAAAA 
GATGCACTGC ATTCTGAAAA GCAGCAAATT GCCACAGAGA AACAGGACCT GGAAGCAGAG 
GTGTCCCAGC TCACAGGAGA GGTTGCCAAG CTGACAAAGG AACTGGAAGA TGCCAAGAAA 
GAAATGGCTT CCCTCTCTGC GGCAGCTATT ACTGTACCTC CTTCTGTTCC TAGTCGTGCT 
CCTGTTCCCC CTGCCCCTCC TTTACCTGGT GACTCTGGCA CTATTATTCC ACCACCACCT 
GCTCCTGGGG ATAGTACCAC TCCTCCTCCT CCTCCTCCTC CTCCTCCTCC TCCACCTCCT 
TTGCCTGGGG GTGTTTGCAT CTCCTCACCC CCTTCTTTAC CTGGAGGTAC TGCTATCTCT 
CCACCCCCTC CTTTGTCTGG GGATGCTACC ATCCCTCCAC CCCCTCCTTT GCCTGAGGGT 
GTTGGCATCC CTTCACCCTC TTCTTTGCCT GGAGGTACTG CCATCCCCCC ACCTCCTCCT 
TTGCCTGGGA GTGCTAGAAT CCCCCCACCA CCACCTCCTT TGCCTGGGAG TGCTGGAATT 
CCCCCCCCAC CTCCTCCCTT GCCTGGAGAA GCAGGAATGC CACCTCCTCC TCCCCCTCTT 
CCTGGTGGTC CTGGAATCCC TCCACCTCCT CCATTTCCCG GAGGCCCTGG CATTCCTCCA 
CCTCCACCCG GAATGGGTAT GCCTCCACCT CCCCCATTTG GATTTGGAGT TCCTGCAGCC 
CCAGTTCTGC CATTTGGATT AACCCCCAAA AAGCTTTATA AGCCAGAGGT GCAGCTCCGG 
AGGCCAAACT GGTCCAAGCT TGTGGCTGAG GACCTCTCCC AGGACTGCTT CTGGACAAAG 
GTGAAGGAGG ACCGCTTTGA GAACAATGAA CTTTTCGCCA AACTTACCCT TACCTTCTCT 
GCCCAGACCA AGACTTCCAA AGCCAAGAAG GATCAAGAAG GTGGAGAAGA AAAGAAATCT 
GTGCAAAAGA AAAAAGTAAA AGAGTTAAAG GTGTTGGATT CAAAGACAGC CCAGAATCTC 
TCAATCTTTT TGGGTTCCTT CCGCATGCCC TATCAAGAGA TTAAGAATGT CATCCTGGAG 
GTGAATGAGG CTGTTCTGAC TGAGTCTATG ATCCAGAACC TCATTAAGCA AATGCCAGAG 
CCAGAGCAGT TAAAAATGCT TTCTGAACTG AAGGATGAAT ATGATGACCT GGCTGAGTCA 
GAGCAGTTTG GCGTGGTGAT GGGCACTGTG CCCCGACTGC GGCCTCGCCT CAATGCCATT 
CTCTTCAAGC TACAATTCAG CGAGCAAGTG GAGAATATCA AGCCAGAGAT TGTGTCTGTC 
ACTGCTGCAT GTGAGGAGTT ACGTAAGAGT GAGAGCTTTT CCAATCTCCT AGAGATTACC 
TTGCTTGTTG GAAATTACAT GAATGCTGGC TCCAGAAATG CTGGTGCTTT TGGCTTCAAT 
ATCAGCTTCC TCTGTAAGCT TCGAGACACC AAGTCCACAG ATCAGAAGAT GACGTTGTTA 
CACTTCTTGG CTGAGTTGTG TGAGAATGAC TATCCCGATG TCCTCAAGTT TCCAGACGAG 
CTTGCCCATG TGGAGAAAGC CAGCCGAGTT TCTGCTGAAA ACTTGCAAAA GAACCTAGAT 
CAGATGAAGA AACAAATTTC TGATGTGGAA CGTGATGTTC AGAATTTCCC AGCTGCCACA 
GATGAAAAAG ACAAGTTTGT TGAAAAAATG ACCAGCTTTG TGAAGGATGC ACAGGAACAG 
TATAACAAGC TGCGGATGAT GCATTCTAAC ATGGAGACCC TCTATAAGGA GCTGGGCGAG 
TACTTCCTCT TTGACCCCAA GAAGTTGTCT GTTGAAGAAT TTTTCATGGA TCTTCACAAT 
TTTCGGAATA TGTTTTTGCA AGCAGTCAAG GAGAACCAGA AGCGGCGGGA GACAGAAGAA 
AAGATGAGGC GAGCAAAACT AGCCAAGGAG AAGGCAGAGA AGGAGCGGCT AGAGAAGCAG 
CAGAAGAGAG AGCAACTCAT AGACATGAAT GCAGAGGGCG ATGAGACAGG TGTGATGGAC 
AGTCTTCTAG AAGCCCTGCA GTCAGGGGCA GCATTCCGAC GGAAGAGAGG GCCCCGTCAA 
GACAACCTCT TGTGCCCCTG GGAAGTAGAG GAGGAGGAGG AGACCTTTAA ATTGCCAACA 
GGAAGGCCGG GTGTGCAGTC ACATCTCTGC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001300936.1
CDS	87..3839
Translation	MEPPGGSLGPGRGTRDKKKGRSPDELPSAGGDGGKSKKFTLKRLMADELERFTSMRIKKEKEKPNSAHRNSSASYGDDPTAQSLQDVSDEQVLVLFEQMLLDMNLNEEKQQPLREKDIIIKREMVSQYLYTSKAGMSQKESSKSAMMYIQELRSGLRDMPLLSCLESLRVSLNNNPVSWVQTFGAEGLASLLDILKRLHDEKEETAGSYDSRNKHEIIRCLKAFMNNKFGIKTMLETEEGILLLVRAMDPAVPNMMIDAAKLLSALCILPQPEDMNERVLEAMTERAEMDEVERFQPLLDGLKSGTTIALKVGCLQLINALITPAEELDFRVHIRSELMRLGLHQVLQDLREIENEDMRVQLNVFDEQGEEDSYDLKGRLDDIRMEMDDFNEVFQILLNTVKDSKAEPHFLSILQHLLLVRNDYEARPQYYKLIEECISQIVLHKNGADPDFKCRHLQIEIEGLIDQMIDKTKVEKSEAKAAELEKKLDSELTARHELQVEMKKMESDFEQKLQDLQGEKDALHSEKQQIATEKQDLEAEVSQLTGEVAKLTKELEDAKKEMASLSAAAITVPPSVPSRAPVPPAPPLPGDSGTIIPPPPAPGDSTTPPPPPPPPPPPPPLPGGVCISSPPSLPGGTAISPPPPLSGDATIPPPPPLPEGVGIPSPSSLPGGTAIPPPPPLPGSARIPPPPPPLPGSAGIPPPPPPLPGEAGMPPPPPPLPGGPGIPPPPPFPGGPGIPPPPPGMGMPPPPPFGFGVPAAPVLPFGLTPKKLYKPEVQLRRPNWSKLVAEDLSQDCFWTKVKEDRFENNELFAKLTLTFSAQTKTSKAKKDQEGGEEKKSVQKKKVKELKVLDSKTAQNLSIFLGSFRMPYQEIKNVILEVNEAVLTESMIQNLIKQMPEPEQLKMLSELKDEYDDLAESEQFGVVMGTVPRLRPRLNAILFKLQFSEQVENIKPEIVSVTAACEELRKSESFSNLLEITLLVGNYMNAGSRNAGAFGFNISFLCKLRDTKSTDQKMTLLHFLAELCENDYPDVLKFPDELAHVEKASRVSAENLQKNLDQMKKQISDVERDVQNFPAATDEKDKFVEKMTSFVKDAQEQYNKLRMMHSNMETLYKELGEYFLFDPKKLSVEEFFMDLHNFRNMFLQAVKENQKRRETEEKMRRAKLAKEKAEKERLEKQQKREQLIDMNAEGDETGVMDSLLEALQSGAAFRRKRGPRQDNLLCPWEVEEEEETFKLPTGRPGVQSHLC

Target ORF information:

RefSeq Version	NM_001314007.2
Organism	Homo sapiens(human)
Definition	Homo sapiens diaphanous related formin 1 (DIAPH1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001314007.2

ORF Insert Sequence:

ATGGAGCCGC CCGGCGGGAG CCTGGGGCCC GGCCGCGGGA CCCGGGACAA GAAGAAGGGC 
CGGAGCCCAG ATGAGCTGCC CTCGGCGGGC GGCGACGGCG GCAAATCTAA GAAATTTACT 
CTGAAGCGGC TCATGGCAGA TGAGCTGGAG AGATTTACCA GCATGAGAAT TAAGAAGGAG 
AAGGAAAAGC CCAATTCTGC TCATAGAAAT TCTTCTGCAT CATATGGGGA TGATCCCACA 
GCACAGTCAT TGCAAGATGT TTCAGATGAA CAAGTGCTGG TTCTCTTTGA ACAGATGCTG 
CTGGATATGA ACCTGAATGA GGAGAAACAG CAACCTTTGA GGGAGAAGGA CATCATCATC 
AAGAGGGAGA TGGTGTCCCA ATACTTGTAC ACCTCCAAGG CTGGCATGAG CCAGAAGGAG 
AGCTCTAAGT CTGCCATGAT GTATATTCAG GAGTTGAGGT CAGGCTTGCG GGATATGCCT 
CTGCTCAGCT GCCTGGAGTC CCTTCGTGTG TCTCTCAACA ACAACCCTGT CAGTTGGGTG 
CAAACATTTG GTGCTGAAGG CTTGGCCTCC TTATTGGACA TTCTTAAACG ACTTCATGAT 
GAGAAAGAAG AGACTGCTGG GAGTTACGAT AGCCGGAACA AGCATGAGAT CATTCGCTGC 
TTGAAAGCTT TTATGAACAA CAAGTTTGGA ATCAAGACCA TGTTGGAGAC AGAAGAAGGA 
ATCCTACTGC TGGTCAGAGC CATGGATCCT GCTGTTCCCA ACATGATGAT TGATGCAGCT 
AAGCTGCTTT CTGCTCTTTG TATTCTACCG CAGCCAGAGG ACATGAATGA AAGGGTTTTG 
GAGGCAATGA CAGAAAGAGC TGAGATGGAT GAAGTGGAAC GTTTCCAGCC GCTGCTGGAT 
GGATTAAAAA GTGGAACCAC TATTGCACTG AAGGTTGGAT GCCTACAGCT GATCAATGCT 
CTCATCACAC CAGCGGAGGA ACTTGACTTC CGAGTTCACA TCAGAAGTGA ACTGATGCGT 
TTGGGGCTAC ATCAGGTGTT GCAGGACCTT CGAGAGATTG AAAATGAAGA TATGAGAGTG 
CAACTAAATG TGTTTGATGA ACAAGGGGAA GAGGATTCCT ATGACCTGAA GGGACGGCTG 
GATGACATTC GCATGGAGAT GGATGACTTT AATGAAGTCT TTCAGATTCT CTTAAACACA 
GTGAAGGATT CAAAGGCAGA GCCACACTTC CTTTCCATCC TGCAGCACTT ACTCTTGGTC 
CGAAATGACT ATGAGGCCAG ACCTCAGTAC TATAAGTTGA TTGAAGAATG TATTTCCCAG 
ATAGTTCTGC ACAAGAACGG GGCTGATCCT GACTTCAAGT GCCGGCACCT CCAGATTGAG 
ATTGAGGGAT TAATTGATCA AATGATTGAT AAGACAAAGG TGGAGAAATC TGAAGCCAAA 
GCTGCAGAGC TGGAAAAGAA GTTGGACTCA GAGTTAACAG CCCGACATGA GCTACAGGTG 
GAAATGAAAA AGATGGAAAG TGACTTTGAG CAGAAGCTTC AAGATCTTCA GGGAGAAAAA 
GATGCACTGC ATTCTGAAAA GCAGCAAATT GCCACAGAGA AACAGGACCT GGAAGCAGAG 
GTGTCCCAGC TCACAGGAGA GGTTGCCAAG CTGACAAAGG AACTGGAAGA TGCCAAGAAA 
GAAATGGCTT CCCTCTCTGC GGCAGCTATT ACTGTACCTC CTTCTGTTCC TAGTCGTGCT 
CCTGTTCCCC CTGCCCCTCC TTTACCTGGT GACTCTGGCA CTATTATTCC ACCACCACCT 
GCTCCTGGGG ATAGTACCAC TCCTCCTCCT CCTCCTCCTC CTCCTCCTCC TCCACCTCCT 
TTGCCTGGGG GTGTTTGCAT CTCCTCACCC CCTTCTTTAC CTGGAGGTAC TGCTATCTCT 
CCACCCCCTC CTTTGTCTGG GGATGCTACC ATCCCTCCAC CCCCTCCTTT GCCTGAGGGT 
GTTGGCATCC CTTCACCCTC TTCTTTGCCT GGAGGTACTG CCATCCCCCC ACCTCCTCCT 
TTGCCTGGGA GTGCTAGAAT CCCCCCACCA CCACCTCCTT TGCCTGGGAG TGCTGGAATT 
CCCCCCCCAC CTCCTCCCTT GCCTGGAGAA GCAGGAATGC CACCTCCTCC TCCCCCTCTT 
CCTGGTGGTC CTGGAATCCC TCCACCTCCT CCATTTCCCG GAGGCCCTGG CATTCCTCCA 
CCTCCACCCG GAATGGGTAT GCCTCCACCT CCCCCATTTG GATTTGGAGT TCCTGCAGCC 
CCAGTTCTGC CATTTGGATT AACCCCCAAA AAGCTTTATA AGCCAGAGGT GCAGCTCCGG 
AGGCCAAACT GGTCCAAGCT TGTGGCTGAG GACCTCTCCC AGGACTGCTT CTGGACAAAG 
GTGAAGGAGG ACCGCTTTGA GAACAATGAA CTTTTCGCCA AACTTACCCT TACCTTCTCT 
GCCCAGACCA AGACTTCCAA AGCCAAGAAG GATCAAGAAG GTGGAGAAGA AAAGAAATCT 
GTGCAAAAGA AAAAAGTAAA AGAGTTAAAG GTGTTGGATT CAAAGACAGC CCAGAATCTC 
TCAATCTTTT TGGGTTCCTT CCGCATGCCC TATCAAGAGA TTAAGAATGT CATCCTGGAG 
GTGAATGAGG CTGTTCTGAC TGAGTCTATG ATCCAGAACC TCATTAAGCA AATGCCAGAG 
CCAGAGCAGT TAAAAATGCT TTCTGAACTG AAGGATGAAT ATGATGACCT GGCTGAGTCA 
GAGCAGTTTG GCGTGGTGAT GGGCACTGTG CCCCGACTGC GGCCTCGCCT CAATGCCATT 
CTCTTCAAGC TACAATTCAG CGAGCAAGTG GAGAATATCA AGCCAGAGAT TGTGTCTGTC 
ACTGCTGCAT GTGAGGAGTT ACGTAAGAGT GAGAGCTTTT CCAATCTCCT AGAGATTACC 
TTGCTTGTTG GAAATTACAT GAATGCTGGC TCCAGAAATG CTGGTGCTTT TGGCTTCAAT 
ATCAGCTTCC TCTGTAAGCT TCGAGACACC AAGTCCACAG ATCAGAAGAT GACGTTGTTA 
CACTTCTTGG CTGAGTTGTG TGAGAATGAC TATCCCGATG TCCTCAAGTT TCCAGACGAG 
CTTGCCCATG TGGAGAAAGC CAGCCGAGTT TCTGCTGAAA ACTTGCAAAA GAACCTAGAT 
CAGATGAAGA AACAAATTTC TGATGTGGAA CGTGATGTTC AGAATTTCCC AGCTGCCACA 
GATGAAAAAG ACAAGTTTGT TGAAAAAATG ACCAGCTTTG TGAAGGATGC ACAGGAACAG 
TATAACAAGC TGCGGATGAT GCATTCTAAC ATGGAGACCC TCTATAAGGA GCTGGGCGAG 
TACTTCCTCT TTGACCCCAA GAAGTTGTCT GTTGAAGAAT TTTTCATGGA TCTTCACAAT 
TTTCGGAATA TGTTTTTGCA AGCAGTCAAG GAGAACCAGA AGCGGCGGGA GACAGAAGAA 
AAGATGAGGC GAGCAAAACT AGCCAAGGAG AAGGCAGAGA AGGAGCGGCT AGAGAAGCAG 
CAGAAGAGAG AGCAACTCAT AGACATGAAT GCAGAGGGCG ATGAGACAGG TGTGATGGAC 
AGTCTTCTAG AAGCCCTGCA GTCAGGGGCA GCATTCCGAC GGAAGAGAGG GCCCCGTCAA 
GACAACCTCT TGTGCCCCTG GGAAGTAGAG GAGGAGGAGG AGACCTTTAA ATTGCCAACA 
GGAAGGCCGG GTGTGCAGTC ACATCTCTGC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DIAPH1 ( XM_011537572.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011535874.1 Homo sapiens diaphanous related formin 1 (DIAPH1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu57238
Clone ID Related Accession (Same CDS sequence)	XM_011537572.2
Accession Version	XM_011537572.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3783bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	protein diaphanous homolog 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011537572.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAGTAC AGAGTCAGTA TGGTAAGTTA GATAGGAGGA AGACTGATTT CTTCTTTTCA 
TGTCAGAAGA GTCTTGAACT GCTTGTGAAC CAGAGGAACC ATGCCTGTCT GGAGAGATTT 
ACCAGCATGA GAATTAAGAA GGAGAAGGAA AAGCCCAATT CTGCTCATAG AAATTCTTCT 
GCATCATATG GGGATGATCC CACAGCACAG TCATTGCAAG ATGTTTCAGA TGAACAAGTG 
CTGGTTCTCT TTGAACAGAT GCTGCTGGAT ATGAACCTGA ATGAGGAGAA ACAGCAACCT 
TTGAGGGAGA AGGACATCAT CATCAAGAGG GAGATGGTGT CCCAATACTT GTACACCTCC 
AAGGCTGGCA TGAGCCAGAA GGAGAGCTCT AAGTCTGCCA TGATGTATAT TCAGGAGTTG 
AGGTCAGGCT TGCGGGATAT GCCTCTGCTC AGCTGCCTGG AGTCCCTTCG TGTGTCTCTC 
AACAACAACC CTGTCAGTTG GGTGCAAACA TTTGGTGCTG AAGGCTTGGC CTCCTTATTG 
GACATTCTTA AACGACTTCA TGATGAGAAA GAAGAGACTG CTGGGAGTTA CGATAGCCGG 
AACAAGCATG AGATCATTCG CTGCTTGAAA GCTTTTATGA ACAACAAGTT TGGAATCAAG 
ACCATGTTGG AGACAGAAGA AGGAATCCTA CTGCTGGTCA GAGCCATGGA TCCTGCTGTT 
CCCAACATGA TGATTGATGC AGCTAAGCTG CTTTCTGCTC TTTGTATTCT ACCGCAGCCA 
GAGGACATGA ATGAAAGGGT TTTGGAGGCA ATGACAGAAA GAGCTGAGAT GGATGAAGTG 
GAACGTTTCC AGCCGCTGCT GGATGGATTA AAAAGTGGAA CCACTATTGC ACTGAAGGTT 
GGATGCCTAC AGCTGATCAA TGCTCTCATC ACACCAGCGG AGGAACTTGA CTTCCGAGTT 
CACATCAGAA GTGAACTGAT GCGTTTGGGG CTACATCAGG TGTTGCAGGA CCTTCGAGAG 
ATTGAAAATG AAGATATGAG AGTGCAACTA AATGTGTTTG ATGAACAAGG GGAAGAGGAT 
TCCTATGACC TGAAGGGACG GCTGGATGAC ATTCGCATGG AGATGGATGA CTTTAATGAA 
GTCTTTCAGA TTCTCTTAAA CACAGTGAAG GATTCAAAGG CAGAGCCACA CTTCCTTTCC 
ATCCTGCAGC ACTTACTCTT GGTCCGAAAT GACTATGAGG CCAGACCTCA GTACTATAAG 
TTGATTGAAG AATGTATTTC CCAGATAGTT CTGCACAAGA ACGGGGCTGA TCCTGACTTC 
AAGTGCCGGC ACCTCCAGAT TGAGATTGAG GGATTAATTG ATCAAATGAT TGATAAGACA 
AAGGTGGAGA AATCTGAAGC CAAAGCTGCA GAGCTGGAAA AGAAGTTGGA CTCAGAGTTA 
ACAGCCCGAC ATGAGCTACA GGTGGAAATG AAAAAGATGG AAAGTGACTT TGAGCAGAAG 
CTTCAAGATC TTCAGGGAGA AAAAGATGCA CTGCATTCTG AAAAGCAGCA AATTGCCACA 
GAGAAACAGG ACCTGGAAGC AGAGGTGTCC CAGCTCACAG GAGAGGTTGC CAAGCTGACA 
AAGGAACTGG AAGATGCCAA GAAAGAAATG GCTTCCCTCT CTGCGGCAGC TATTACTGTA 
CCTCCTTCTG TTCCTAGTCG TGCTCCTGTT CCCCCTGCCC CTCCTTTACC TGGTGACTCT 
GGCACTATTA TTCCACCACC ACCTGCTCCT GGGGATAGTA CCACTCCTCC TCCTCCTCCT 
CCTCCTCCTC CTCCTCCACC TCCTTTGCCT GGGGGTGTTT GCATCTCCTC ACCCCCTTCT 
TTACCTGGAG GTACTGCTAT CTCTCCACCC CCTCCTTTGT CTGGGGATGC TACCATCCCT 
CCACCCCCTC CTTTGCCTGA GGGTGTTGGC ATCCCTTCAC CCTCTTCTTT GCCTGGAGGT 
ACTGCCATCC CCCCACCTCC TCCTTTGCCT GGGAGTGCTA GAATCCCCCC ACCACCACCT 
CCTTTGCCTG GGAGTGCTGG AATTCCCCCC CCACCTCCTC CCTTGCCTGG AGAAGCAGGA 
ATGCCACCTC CTCCTCCCCC TCTTCCTGGT GGTCCTGGAA TCCCTCCACC TCCTCCATTT 
CCCGGAGGCC CTGGCATTCC TCCACCTCCA CCCGGAATGG GTATGCCTCC ACCTCCCCCA 
TTTGGATTTG GAGTTCCTGC AGCCCCAGTT CTGCCATTTG GATTAACCCC CAAAAAGCTT 
TATAAGCCAG AGGTGCAGCT CCGGAGGCCA AACTGGTCCA AGCTTGTGGC TGAGGACCTC 
TCCCAGGACT GCTTCTGGAC AAAGGTGAAG GAGGACCGCT TTGAGAACAA TGAACTTTTC 
GCCAAACTTA CCCTTACCTT CTCTGCCCAG ACCAAGACTT CCAAAGCCAA GAAGGATCAA 
GAAGGTGGAG AAGAAAAGAA ATCTGTGCAA AAGAAAAAAG TAAAAGAGTT AAAGGTGTTG 
GATTCAAAGA CAGCCCAGAA TCTCTCAATC TTTTTGGGTT CCTTCCGCAT GCCCTATCAA 
GAGATTAAGA ATGTCATCCT GGAGGTGAAT GAGGCTGTTC TGACTGAGTC TATGATCCAG 
AACCTCATTA AGCAAATGCC AGAGCCAGAG CAGTTAAAAA TGCTTTCTGA ACTGAAGGAT 
GAATATGATG ACCTGGCTGA GTCAGAGCAG TTTGGCGTGG TGATGGGCAC TGTGCCCCGA 
CTGCGGCCTC GCCTCAATGC CATTCTCTTC AAGCTACAAT TCAGCGAGCA AGTGGAGAAT 
ATCAAGCCAG AGATTGTGTC TGTCACTGCT GCATGTGAGG AGTTACGTAA GAGTGAGAGC 
TTTTCCAATC TCCTAGAGAT TACCTTGCTT GTTGGAAATT ACATGAATGC TGGCTCCAGA 
AATGCTGGTG CTTTTGGCTT CAATATCAGC TTCCTCTGTA AGCTTCGAGA CACCAAGTCC 
ACAGATCAGA AGATGACGTT GTTACACTTC TTGGCTGAGT TGTGTGAGAA TGACTATCCC 
GATGTCCTCA AGTTTCCAGA CGAGCTTGCC CATGTGGAGA AAGCCAGCCG AGTTTCTGCT 
GAAAACTTGC AAAAGAACCT AGATCAGATG AAGAAACAAA TTTCTGATGT GGAACGTGAT 
GTTCAGAATT TCCCAGCTGC CACAGATGAA AAAGACAAGT TTGTTGAAAA AATGACCAGC 
TTTGTGAAGG ATGCACAGGA ACAGTATAAC AAGCTGCGGA TGATGCATTC TAACATGGAG 
ACCCTCTATA AGGAGCTGGG CGAGTACTTC CTCTTTGACC CCAAGAAGTT GTCTGTTGAA 
GAATTTTTCA TGGATCTTCA CAATTTTCGG AATATGTTTT TGCAAGCAGT CAAGGAGAAC 
CAGAAGCGGC GGGAGACAGA AGAAAAGATG AGGCGAGCAA AACTAGCCAA GGAGAAGGCA 
GAGAAGGAGC GGCTAGAGAA GCAGCAGAAG AGAGAGCAAC TCATAGACAT GAATGCAGAG 
GGCGATGAGA CAGGTGTGAT GGACAGTCTT CTAGAAGCCC TGCAGTCAGG GGCAGCATTC 
CGACGGAAGA GAGGGCCCCG TCAAGCCAAC AGGAAGGCCG GGTGTGCAGT CACATCTCTG 
CTAGCTTCGG AGCTGACCAA GGATGATGCC ATGGCTGCTG TTCCTGCCAA GGTGTCCAAG 
AACAGTGAGA CATTCCCCAC AATCCTTGAG GAAGCCAAGG AGTTGGTTGG CCGTGCAAGC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011535874.1
CDS	119..3901
Translation	MRVQSQYGKLDRRKTDFFFSCQKSLELLVNQRNHACLERFTSMRIKKEKEKPNSAHRNSSASYGDDPTAQSLQDVSDEQVLVLFEQMLLDMNLNEEKQQPLREKDIIIKREMVSQYLYTSKAGMSQKESSKSAMMYIQELRSGLRDMPLLSCLESLRVSLNNNPVSWVQTFGAEGLASLLDILKRLHDEKEETAGSYDSRNKHEIIRCLKAFMNNKFGIKTMLETEEGILLLVRAMDPAVPNMMIDAAKLLSALCILPQPEDMNERVLEAMTERAEMDEVERFQPLLDGLKSGTTIALKVGCLQLINALITPAEELDFRVHIRSELMRLGLHQVLQDLREIENEDMRVQLNVFDEQGEEDSYDLKGRLDDIRMEMDDFNEVFQILLNTVKDSKAEPHFLSILQHLLLVRNDYEARPQYYKLIEECISQIVLHKNGADPDFKCRHLQIEIEGLIDQMIDKTKVEKSEAKAAELEKKLDSELTARHELQVEMKKMESDFEQKLQDLQGEKDALHSEKQQIATEKQDLEAEVSQLTGEVAKLTKELEDAKKEMASLSAAAITVPPSVPSRAPVPPAPPLPGDSGTIIPPPPAPGDSTTPPPPPPPPPPPPPLPGGVCISSPPSLPGGTAISPPPPLSGDATIPPPPPLPEGVGIPSPSSLPGGTAIPPPPPLPGSARIPPPPPPLPGSAGIPPPPPPLPGEAGMPPPPPPLPGGPGIPPPPPFPGGPGIPPPPPGMGMPPPPPFGFGVPAAPVLPFGLTPKKLYKPEVQLRRPNWSKLVAEDLSQDCFWTKVKEDRFENNELFAKLTLTFSAQTKTSKAKKDQEGGEEKKSVQKKKVKELKVLDSKTAQNLSIFLGSFRMPYQEIKNVILEVNEAVLTESMIQNLIKQMPEPEQLKMLSELKDEYDDLAESEQFGVVMGTVPRLRPRLNAILFKLQFSEQVENIKPEIVSVTAACEELRKSESFSNLLEITLLVGNYMNAGSRNAGAFGFNISFLCKLRDTKSTDQKMTLLHFLAELCENDYPDVLKFPDELAHVEKASRVSAENLQKNLDQMKKQISDVERDVQNFPAATDEKDKFVEKMTSFVKDAQEQYNKLRMMHSNMETLYKELGEYFLFDPKKLSVEEFFMDLHNFRNMFLQAVKENQKRRETEEKMRRAKLAKEKAEKERLEKQQKREQLIDMNAEGDETGVMDSLLEALQSGAAFRRKRGPRQANRKAGCAVTSLLASELTKDDAMAAVPAKVSKNSETFPTILEEAKELVGRAS

Target ORF information:

RefSeq Version	XM_011537572.2
Organism	Homo sapiens(human)
Definition	Homo sapiens diaphanous related formin 1 (DIAPH1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011537572.2

ORF Insert Sequence:

ATGAGAGTAC AGAGTCAGTA TGGTAAGTTA GATAGGAGGA AGACTGATTT CTTCTTTTCA 
TGTCAGAAGA GTCTTGAACT GCTTGTGAAC CAGAGGAACC ATGCCTGTCT GGAGAGATTT 
ACCAGCATGA GAATTAAGAA GGAGAAGGAA AAGCCCAATT CTGCTCATAG AAATTCTTCT 
GCATCATATG GGGATGATCC CACAGCACAG TCATTGCAAG ATGTTTCAGA TGAACAAGTG 
CTGGTTCTCT TTGAACAGAT GCTGCTGGAT ATGAACCTGA ATGAGGAGAA ACAGCAACCT 
TTGAGGGAGA AGGACATCAT CATCAAGAGG GAGATGGTGT CCCAATACTT GTACACCTCC 
AAGGCTGGCA TGAGCCAGAA GGAGAGCTCT AAGTCTGCCA TGATGTATAT TCAGGAGTTG 
AGGTCAGGCT TGCGGGATAT GCCTCTGCTC AGCTGCCTGG AGTCCCTTCG TGTGTCTCTC 
AACAACAACC CTGTCAGTTG GGTGCAAACA TTTGGTGCTG AAGGCTTGGC CTCCTTATTG 
GACATTCTTA AACGACTTCA TGATGAGAAA GAAGAGACTG CTGGGAGTTA CGATAGCCGG 
AACAAGCATG AGATCATTCG CTGCTTGAAA GCTTTTATGA ACAACAAGTT TGGAATCAAG 
ACCATGTTGG AGACAGAAGA AGGAATCCTA CTGCTGGTCA GAGCCATGGA TCCTGCTGTT 
CCCAACATGA TGATTGATGC AGCTAAGCTG CTTTCTGCTC TTTGTATTCT ACCGCAGCCA 
GAGGACATGA ATGAAAGGGT TTTGGAGGCA ATGACAGAAA GAGCTGAGAT GGATGAAGTG 
GAACGTTTCC AGCCGCTGCT GGATGGATTA AAAAGTGGAA CCACTATTGC ACTGAAGGTT 
GGATGCCTAC AGCTGATCAA TGCTCTCATC ACACCAGCGG AGGAACTTGA CTTCCGAGTT 
CACATCAGAA GTGAACTGAT GCGTTTGGGG CTACATCAGG TGTTGCAGGA CCTTCGAGAG 
ATTGAAAATG AAGATATGAG AGTGCAACTA AATGTGTTTG ATGAACAAGG GGAAGAGGAT 
TCCTATGACC TGAAGGGACG GCTGGATGAC ATTCGCATGG AGATGGATGA CTTTAATGAA 
GTCTTTCAGA TTCTCTTAAA CACAGTGAAG GATTCAAAGG CAGAGCCACA CTTCCTTTCC 
ATCCTGCAGC ACTTACTCTT GGTCCGAAAT GACTATGAGG CCAGACCTCA GTACTATAAG 
TTGATTGAAG AATGTATTTC CCAGATAGTT CTGCACAAGA ACGGGGCTGA TCCTGACTTC 
AAGTGCCGGC ACCTCCAGAT TGAGATTGAG GGATTAATTG ATCAAATGAT TGATAAGACA 
AAGGTGGAGA AATCTGAAGC CAAAGCTGCA GAGCTGGAAA AGAAGTTGGA CTCAGAGTTA 
ACAGCCCGAC ATGAGCTACA GGTGGAAATG AAAAAGATGG AAAGTGACTT TGAGCAGAAG 
CTTCAAGATC TTCAGGGAGA AAAAGATGCA CTGCATTCTG AAAAGCAGCA AATTGCCACA 
GAGAAACAGG ACCTGGAAGC AGAGGTGTCC CAGCTCACAG GAGAGGTTGC CAAGCTGACA 
AAGGAACTGG AAGATGCCAA GAAAGAAATG GCTTCCCTCT CTGCGGCAGC TATTACTGTA 
CCTCCTTCTG TTCCTAGTCG TGCTCCTGTT CCCCCTGCCC CTCCTTTACC TGGTGACTCT 
GGCACTATTA TTCCACCACC ACCTGCTCCT GGGGATAGTA CCACTCCTCC TCCTCCTCCT 
CCTCCTCCTC CTCCTCCACC TCCTTTGCCT GGGGGTGTTT GCATCTCCTC ACCCCCTTCT 
TTACCTGGAG GTACTGCTAT CTCTCCACCC CCTCCTTTGT CTGGGGATGC TACCATCCCT 
CCACCCCCTC CTTTGCCTGA GGGTGTTGGC ATCCCTTCAC CCTCTTCTTT GCCTGGAGGT 
ACTGCCATCC CCCCACCTCC TCCTTTGCCT GGGAGTGCTA GAATCCCCCC ACCACCACCT 
CCTTTGCCTG GGAGTGCTGG AATTCCCCCC CCACCTCCTC CCTTGCCTGG AGAAGCAGGA 
ATGCCACCTC CTCCTCCCCC TCTTCCTGGT GGTCCTGGAA TCCCTCCACC TCCTCCATTT 
CCCGGAGGCC CTGGCATTCC TCCACCTCCA CCCGGAATGG GTATGCCTCC ACCTCCCCCA 
TTTGGATTTG GAGTTCCTGC AGCCCCAGTT CTGCCATTTG GATTAACCCC CAAAAAGCTT 
TATAAGCCAG AGGTGCAGCT CCGGAGGCCA AACTGGTCCA AGCTTGTGGC TGAGGACCTC 
TCCCAGGACT GCTTCTGGAC AAAGGTGAAG GAGGACCGCT TTGAGAACAA TGAACTTTTC 
GCCAAACTTA CCCTTACCTT CTCTGCCCAG ACCAAGACTT CCAAAGCCAA GAAGGATCAA 
GAAGGTGGAG AAGAAAAGAA ATCTGTGCAA AAGAAAAAAG TAAAAGAGTT AAAGGTGTTG 
GATTCAAAGA CAGCCCAGAA TCTCTCAATC TTTTTGGGTT CCTTCCGCAT GCCCTATCAA 
GAGATTAAGA ATGTCATCCT GGAGGTGAAT GAGGCTGTTC TGACTGAGTC TATGATCCAG 
AACCTCATTA AGCAAATGCC AGAGCCAGAG CAGTTAAAAA TGCTTTCTGA ACTGAAGGAT 
GAATATGATG ACCTGGCTGA GTCAGAGCAG TTTGGCGTGG TGATGGGCAC TGTGCCCCGA 
CTGCGGCCTC GCCTCAATGC CATTCTCTTC AAGCTACAAT TCAGCGAGCA AGTGGAGAAT 
ATCAAGCCAG AGATTGTGTC TGTCACTGCT GCATGTGAGG AGTTACGTAA GAGTGAGAGC 
TTTTCCAATC TCCTAGAGAT TACCTTGCTT GTTGGAAATT ACATGAATGC TGGCTCCAGA 
AATGCTGGTG CTTTTGGCTT CAATATCAGC TTCCTCTGTA AGCTTCGAGA CACCAAGTCC 
ACAGATCAGA AGATGACGTT GTTACACTTC TTGGCTGAGT TGTGTGAGAA TGACTATCCC 
GATGTCCTCA AGTTTCCAGA CGAGCTTGCC CATGTGGAGA AAGCCAGCCG AGTTTCTGCT 
GAAAACTTGC AAAAGAACCT AGATCAGATG AAGAAACAAA TTTCTGATGT GGAACGTGAT 
GTTCAGAATT TCCCAGCTGC CACAGATGAA AAAGACAAGT TTGTTGAAAA AATGACCAGC 
TTTGTGAAGG ATGCACAGGA ACAGTATAAC AAGCTGCGGA TGATGCATTC TAACATGGAG 
ACCCTCTATA AGGAGCTGGG CGAGTACTTC CTCTTTGACC CCAAGAAGTT GTCTGTTGAA 
GAATTTTTCA TGGATCTTCA CAATTTTCGG AATATGTTTT TGCAAGCAGT CAAGGAGAAC 
CAGAAGCGGC GGGAGACAGA AGAAAAGATG AGGCGAGCAA AACTAGCCAA GGAGAAGGCA 
GAGAAGGAGC GGCTAGAGAA GCAGCAGAAG AGAGAGCAAC TCATAGACAT GAATGCAGAG 
GGCGATGAGA CAGGTGTGAT GGACAGTCTT CTAGAAGCCC TGCAGTCAGG GGCAGCATTC 
CGACGGAAGA GAGGGCCCCG TCAAGCCAAC AGGAAGGCCG GGTGTGCAGT CACATCTCTG 
CTAGCTTCGG AGCTGACCAA GGATGATGCC ATGGCTGCTG TTCCTGCCAA GGTGTCCAAG 
AACAGTGAGA CATTCCCCAC AATCCTTGAG GAAGCCAAGG AGTTGGTTGG CCGTGCAAGC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DIAPH1 ( XM_011537573.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011535875.1 Homo sapiens diaphanous related formin 1 (DIAPH1), transcript variant X2, mRNA.

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CloneID	OHu57239
Clone ID Related Accession (Same CDS sequence)	XM_011537573.2
Accession Version	XM_011537573.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3753bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	protein diaphanous homolog 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011537573.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGATAA ACCTCTCCTG TGGGAAGAAA GTTGGACTGC GAGTTCAAGA AACTCTGAAG 
CGGCTCATGG CAGATGAGCT GGAGAGATTT ACCAGCATGA GAATTAAGAA GGAGAAGGAA 
AAGCCCAATT CTGCTCATAG AAATTCTTCT GCATCATATG GGGATGATCC CACAGCACAG 
TCATTGCAAG ATGTTTCAGA TGAACAAGTG CTGGTTCTCT TTGAACAGAT GCTGCTGGAT 
ATGAACCTGA ATGAGGAGAA ACAGCAACCT TTGAGGGAGA AGGACATCAT CATCAAGAGG 
GAGATGGTGT CCCAATACTT GTACACCTCC AAGGCTGGCA TGAGCCAGAA GGAGAGCTCT 
AAGTCTGCCA TGATGTATAT TCAGGAGTTG AGGTCAGGCT TGCGGGATAT GCCTCTGCTC 
AGCTGCCTGG AGTCCCTTCG TGTGTCTCTC AACAACAACC CTGTCAGTTG GGTGCAAACA 
TTTGGTGCTG AAGGCTTGGC CTCCTTATTG GACATTCTTA AACGACTTCA TGATGAGAAA 
GAAGAGACTG CTGGGAGTTA CGATAGCCGG AACAAGCATG AGATCATTCG CTGCTTGAAA 
GCTTTTATGA ACAACAAGTT TGGAATCAAG ACCATGTTGG AGACAGAAGA AGGAATCCTA 
CTGCTGGTCA GAGCCATGGA TCCTGCTGTT CCCAACATGA TGATTGATGC AGCTAAGCTG 
CTTTCTGCTC TTTGTATTCT ACCGCAGCCA GAGGACATGA ATGAAAGGGT TTTGGAGGCA 
ATGACAGAAA GAGCTGAGAT GGATGAAGTG GAACGTTTCC AGCCGCTGCT GGATGGATTA 
AAAAGTGGAA CCACTATTGC ACTGAAGGTT GGATGCCTAC AGCTGATCAA TGCTCTCATC 
ACACCAGCGG AGGAACTTGA CTTCCGAGTT CACATCAGAA GTGAACTGAT GCGTTTGGGG 
CTACATCAGG TGTTGCAGGA CCTTCGAGAG ATTGAAAATG AAGATATGAG AGTGCAACTA 
AATGTGTTTG ATGAACAAGG GGAAGAGGAT TCCTATGACC TGAAGGGACG GCTGGATGAC 
ATTCGCATGG AGATGGATGA CTTTAATGAA GTCTTTCAGA TTCTCTTAAA CACAGTGAAG 
GATTCAAAGG CAGAGCCACA CTTCCTTTCC ATCCTGCAGC ACTTACTCTT GGTCCGAAAT 
GACTATGAGG CCAGACCTCA GTACTATAAG TTGATTGAAG AATGTATTTC CCAGATAGTT 
CTGCACAAGA ACGGGGCTGA TCCTGACTTC AAGTGCCGGC ACCTCCAGAT TGAGATTGAG 
GGATTAATTG ATCAAATGAT TGATAAGACA AAGGTGGAGA AATCTGAAGC CAAAGCTGCA 
GAGCTGGAAA AGAAGTTGGA CTCAGAGTTA ACAGCCCGAC ATGAGCTACA GGTGGAAATG 
AAAAAGATGG AAAGTGACTT TGAGCAGAAG CTTCAAGATC TTCAGGGAGA AAAAGATGCA 
CTGCATTCTG AAAAGCAGCA AATTGCCACA GAGAAACAGG ACCTGGAAGC AGAGGTGTCC 
CAGCTCACAG GAGAGGTTGC CAAGCTGACA AAGGAACTGG AAGATGCCAA GAAAGAAATG 
GCTTCCCTCT CTGCGGCAGC TATTACTGTA CCTCCTTCTG TTCCTAGTCG TGCTCCTGTT 
CCCCCTGCCC CTCCTTTACC TGGTGACTCT GGCACTATTA TTCCACCACC ACCTGCTCCT 
GGGGATAGTA CCACTCCTCC TCCTCCTCCT CCTCCTCCTC CTCCTCCACC TCCTTTGCCT 
GGGGGTGTTT GCATCTCCTC ACCCCCTTCT TTACCTGGAG GTACTGCTAT CTCTCCACCC 
CCTCCTTTGT CTGGGGATGC TACCATCCCT CCACCCCCTC CTTTGCCTGA GGGTGTTGGC 
ATCCCTTCAC CCTCTTCTTT GCCTGGAGGT ACTGCCATCC CCCCACCTCC TCCTTTGCCT 
GGGAGTGCTA GAATCCCCCC ACCACCACCT CCTTTGCCTG GGAGTGCTGG AATTCCCCCC 
CCACCTCCTC CCTTGCCTGG AGAAGCAGGA ATGCCACCTC CTCCTCCCCC TCTTCCTGGT 
GGTCCTGGAA TCCCTCCACC TCCTCCATTT CCCGGAGGCC CTGGCATTCC TCCACCTCCA 
CCCGGAATGG GTATGCCTCC ACCTCCCCCA TTTGGATTTG GAGTTCCTGC AGCCCCAGTT 
CTGCCATTTG GATTAACCCC CAAAAAGCTT TATAAGCCAG AGGTGCAGCT CCGGAGGCCA 
AACTGGTCCA AGCTTGTGGC TGAGGACCTC TCCCAGGACT GCTTCTGGAC AAAGGTGAAG 
GAGGACCGCT TTGAGAACAA TGAACTTTTC GCCAAACTTA CCCTTACCTT CTCTGCCCAG 
ACCAAGACTT CCAAAGCCAA GAAGGATCAA GAAGGTGGAG AAGAAAAGAA ATCTGTGCAA 
AAGAAAAAAG TAAAAGAGTT AAAGGTGTTG GATTCAAAGA CAGCCCAGAA TCTCTCAATC 
TTTTTGGGTT CCTTCCGCAT GCCCTATCAA GAGATTAAGA ATGTCATCCT GGAGGTGAAT 
GAGGCTGTTC TGACTGAGTC TATGATCCAG AACCTCATTA AGCAAATGCC AGAGCCAGAG 
CAGTTAAAAA TGCTTTCTGA ACTGAAGGAT GAATATGATG ACCTGGCTGA GTCAGAGCAG 
TTTGGCGTGG TGATGGGCAC TGTGCCCCGA CTGCGGCCTC GCCTCAATGC CATTCTCTTC 
AAGCTACAAT TCAGCGAGCA AGTGGAGAAT ATCAAGCCAG AGATTGTGTC TGTCACTGCT 
GCATGTGAGG AGTTACGTAA GAGTGAGAGC TTTTCCAATC TCCTAGAGAT TACCTTGCTT 
GTTGGAAATT ACATGAATGC TGGCTCCAGA AATGCTGGTG CTTTTGGCTT CAATATCAGC 
TTCCTCTGTA AGCTTCGAGA CACCAAGTCC ACAGATCAGA AGATGACGTT GTTACACTTC 
TTGGCTGAGT TGTGTGAGAA TGACTATCCC GATGTCCTCA AGTTTCCAGA CGAGCTTGCC 
CATGTGGAGA AAGCCAGCCG AGTTTCTGCT GAAAACTTGC AAAAGAACCT AGATCAGATG 
AAGAAACAAA TTTCTGATGT GGAACGTGAT GTTCAGAATT TCCCAGCTGC CACAGATGAA 
AAAGACAAGT TTGTTGAAAA AATGACCAGC TTTGTGAAGG ATGCACAGGA ACAGTATAAC 
AAGCTGCGGA TGATGCATTC TAACATGGAG ACCCTCTATA AGGAGCTGGG CGAGTACTTC 
CTCTTTGACC CCAAGAAGTT GTCTGTTGAA GAATTTTTCA TGGATCTTCA CAATTTTCGG 
AATATGTTTT TGCAAGCAGT CAAGGAGAAC CAGAAGCGGC GGGAGACAGA AGAAAAGATG 
AGGCGAGCAA AACTAGCCAA GGAGAAGGCA GAGAAGGAGC GGCTAGAGAA GCAGCAGAAG 
AGAGAGCAAC TCATAGACAT GAATGCAGAG GGCGATGAGA CAGGTGTGAT GGACAGTCTT 
CTAGAAGCCC TGCAGTCAGG GGCAGCATTC CGACGGAAGA GAGGGCCCCG TCAAGCCAAC 
AGGAAGGCCG GGTGTGCAGT CACATCTCTG CTAGCTTCGG AGCTGACCAA GGATGATGCC 
ATGGCTGCTG TTCCTGCCAA GGTGTCCAAG AACAGTGAGA CATTCCCCAC AATCCTTGAG 
GAAGCCAAGG AGTTGGTTGG CCGTGCAAGC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011535875.1
CDS	16..3768
Translation	MVINLSCGKKVGLRVQETLKRLMADELERFTSMRIKKEKEKPNSAHRNSSASYGDDPTAQSLQDVSDEQVLVLFEQMLLDMNLNEEKQQPLREKDIIIKREMVSQYLYTSKAGMSQKESSKSAMMYIQELRSGLRDMPLLSCLESLRVSLNNNPVSWVQTFGAEGLASLLDILKRLHDEKEETAGSYDSRNKHEIIRCLKAFMNNKFGIKTMLETEEGILLLVRAMDPAVPNMMIDAAKLLSALCILPQPEDMNERVLEAMTERAEMDEVERFQPLLDGLKSGTTIALKVGCLQLINALITPAEELDFRVHIRSELMRLGLHQVLQDLREIENEDMRVQLNVFDEQGEEDSYDLKGRLDDIRMEMDDFNEVFQILLNTVKDSKAEPHFLSILQHLLLVRNDYEARPQYYKLIEECISQIVLHKNGADPDFKCRHLQIEIEGLIDQMIDKTKVEKSEAKAAELEKKLDSELTARHELQVEMKKMESDFEQKLQDLQGEKDALHSEKQQIATEKQDLEAEVSQLTGEVAKLTKELEDAKKEMASLSAAAITVPPSVPSRAPVPPAPPLPGDSGTIIPPPPAPGDSTTPPPPPPPPPPPPPLPGGVCISSPPSLPGGTAISPPPPLSGDATIPPPPPLPEGVGIPSPSSLPGGTAIPPPPPLPGSARIPPPPPPLPGSAGIPPPPPPLPGEAGMPPPPPPLPGGPGIPPPPPFPGGPGIPPPPPGMGMPPPPPFGFGVPAAPVLPFGLTPKKLYKPEVQLRRPNWSKLVAEDLSQDCFWTKVKEDRFENNELFAKLTLTFSAQTKTSKAKKDQEGGEEKKSVQKKKVKELKVLDSKTAQNLSIFLGSFRMPYQEIKNVILEVNEAVLTESMIQNLIKQMPEPEQLKMLSELKDEYDDLAESEQFGVVMGTVPRLRPRLNAILFKLQFSEQVENIKPEIVSVTAACEELRKSESFSNLLEITLLVGNYMNAGSRNAGAFGFNISFLCKLRDTKSTDQKMTLLHFLAELCENDYPDVLKFPDELAHVEKASRVSAENLQKNLDQMKKQISDVERDVQNFPAATDEKDKFVEKMTSFVKDAQEQYNKLRMMHSNMETLYKELGEYFLFDPKKLSVEEFFMDLHNFRNMFLQAVKENQKRRETEEKMRRAKLAKEKAEKERLEKQQKREQLIDMNAEGDETGVMDSLLEALQSGAAFRRKRGPRQANRKAGCAVTSLLASELTKDDAMAAVPAKVSKNSETFPTILEEAKELVGRAS

Target ORF information:

RefSeq Version	XM_011537573.2
Organism	Homo sapiens(human)
Definition	Homo sapiens diaphanous related formin 1 (DIAPH1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011537573.2

ORF Insert Sequence:

ATGGTGATAA ACCTCTCCTG TGGGAAGAAA GTTGGACTGC GAGTTCAAGA AACTCTGAAG 
CGGCTCATGG CAGATGAGCT GGAGAGATTT ACCAGCATGA GAATTAAGAA GGAGAAGGAA 
AAGCCCAATT CTGCTCATAG AAATTCTTCT GCATCATATG GGGATGATCC CACAGCACAG 
TCATTGCAAG ATGTTTCAGA TGAACAAGTG CTGGTTCTCT TTGAACAGAT GCTGCTGGAT 
ATGAACCTGA ATGAGGAGAA ACAGCAACCT TTGAGGGAGA AGGACATCAT CATCAAGAGG 
GAGATGGTGT CCCAATACTT GTACACCTCC AAGGCTGGCA TGAGCCAGAA GGAGAGCTCT 
AAGTCTGCCA TGATGTATAT TCAGGAGTTG AGGTCAGGCT TGCGGGATAT GCCTCTGCTC 
AGCTGCCTGG AGTCCCTTCG TGTGTCTCTC AACAACAACC CTGTCAGTTG GGTGCAAACA 
TTTGGTGCTG AAGGCTTGGC CTCCTTATTG GACATTCTTA AACGACTTCA TGATGAGAAA 
GAAGAGACTG CTGGGAGTTA CGATAGCCGG AACAAGCATG AGATCATTCG CTGCTTGAAA 
GCTTTTATGA ACAACAAGTT TGGAATCAAG ACCATGTTGG AGACAGAAGA AGGAATCCTA 
CTGCTGGTCA GAGCCATGGA TCCTGCTGTT CCCAACATGA TGATTGATGC AGCTAAGCTG 
CTTTCTGCTC TTTGTATTCT ACCGCAGCCA GAGGACATGA ATGAAAGGGT TTTGGAGGCA 
ATGACAGAAA GAGCTGAGAT GGATGAAGTG GAACGTTTCC AGCCGCTGCT GGATGGATTA 
AAAAGTGGAA CCACTATTGC ACTGAAGGTT GGATGCCTAC AGCTGATCAA TGCTCTCATC 
ACACCAGCGG AGGAACTTGA CTTCCGAGTT CACATCAGAA GTGAACTGAT GCGTTTGGGG 
CTACATCAGG TGTTGCAGGA CCTTCGAGAG ATTGAAAATG AAGATATGAG AGTGCAACTA 
AATGTGTTTG ATGAACAAGG GGAAGAGGAT TCCTATGACC TGAAGGGACG GCTGGATGAC 
ATTCGCATGG AGATGGATGA CTTTAATGAA GTCTTTCAGA TTCTCTTAAA CACAGTGAAG 
GATTCAAAGG CAGAGCCACA CTTCCTTTCC ATCCTGCAGC ACTTACTCTT GGTCCGAAAT 
GACTATGAGG CCAGACCTCA GTACTATAAG TTGATTGAAG AATGTATTTC CCAGATAGTT 
CTGCACAAGA ACGGGGCTGA TCCTGACTTC AAGTGCCGGC ACCTCCAGAT TGAGATTGAG 
GGATTAATTG ATCAAATGAT TGATAAGACA AAGGTGGAGA AATCTGAAGC CAAAGCTGCA 
GAGCTGGAAA AGAAGTTGGA CTCAGAGTTA ACAGCCCGAC ATGAGCTACA GGTGGAAATG 
AAAAAGATGG AAAGTGACTT TGAGCAGAAG CTTCAAGATC TTCAGGGAGA AAAAGATGCA 
CTGCATTCTG AAAAGCAGCA AATTGCCACA GAGAAACAGG ACCTGGAAGC AGAGGTGTCC 
CAGCTCACAG GAGAGGTTGC CAAGCTGACA AAGGAACTGG AAGATGCCAA GAAAGAAATG 
GCTTCCCTCT CTGCGGCAGC TATTACTGTA CCTCCTTCTG TTCCTAGTCG TGCTCCTGTT 
CCCCCTGCCC CTCCTTTACC TGGTGACTCT GGCACTATTA TTCCACCACC ACCTGCTCCT 
GGGGATAGTA CCACTCCTCC TCCTCCTCCT CCTCCTCCTC CTCCTCCACC TCCTTTGCCT 
GGGGGTGTTT GCATCTCCTC ACCCCCTTCT TTACCTGGAG GTACTGCTAT CTCTCCACCC 
CCTCCTTTGT CTGGGGATGC TACCATCCCT CCACCCCCTC CTTTGCCTGA GGGTGTTGGC 
ATCCCTTCAC CCTCTTCTTT GCCTGGAGGT ACTGCCATCC CCCCACCTCC TCCTTTGCCT 
GGGAGTGCTA GAATCCCCCC ACCACCACCT CCTTTGCCTG GGAGTGCTGG AATTCCCCCC 
CCACCTCCTC CCTTGCCTGG AGAAGCAGGA ATGCCACCTC CTCCTCCCCC TCTTCCTGGT 
GGTCCTGGAA TCCCTCCACC TCCTCCATTT CCCGGAGGCC CTGGCATTCC TCCACCTCCA 
CCCGGAATGG GTATGCCTCC ACCTCCCCCA TTTGGATTTG GAGTTCCTGC AGCCCCAGTT 
CTGCCATTTG GATTAACCCC CAAAAAGCTT TATAAGCCAG AGGTGCAGCT CCGGAGGCCA 
AACTGGTCCA AGCTTGTGGC TGAGGACCTC TCCCAGGACT GCTTCTGGAC AAAGGTGAAG 
GAGGACCGCT TTGAGAACAA TGAACTTTTC GCCAAACTTA CCCTTACCTT CTCTGCCCAG 
ACCAAGACTT CCAAAGCCAA GAAGGATCAA GAAGGTGGAG AAGAAAAGAA ATCTGTGCAA 
AAGAAAAAAG TAAAAGAGTT AAAGGTGTTG GATTCAAAGA CAGCCCAGAA TCTCTCAATC 
TTTTTGGGTT CCTTCCGCAT GCCCTATCAA GAGATTAAGA ATGTCATCCT GGAGGTGAAT 
GAGGCTGTTC TGACTGAGTC TATGATCCAG AACCTCATTA AGCAAATGCC AGAGCCAGAG 
CAGTTAAAAA TGCTTTCTGA ACTGAAGGAT GAATATGATG ACCTGGCTGA GTCAGAGCAG 
TTTGGCGTGG TGATGGGCAC TGTGCCCCGA CTGCGGCCTC GCCTCAATGC CATTCTCTTC 
AAGCTACAAT TCAGCGAGCA AGTGGAGAAT ATCAAGCCAG AGATTGTGTC TGTCACTGCT 
GCATGTGAGG AGTTACGTAA GAGTGAGAGC TTTTCCAATC TCCTAGAGAT TACCTTGCTT 
GTTGGAAATT ACATGAATGC TGGCTCCAGA AATGCTGGTG CTTTTGGCTT CAATATCAGC 
TTCCTCTGTA AGCTTCGAGA CACCAAGTCC ACAGATCAGA AGATGACGTT GTTACACTTC 
TTGGCTGAGT TGTGTGAGAA TGACTATCCC GATGTCCTCA AGTTTCCAGA CGAGCTTGCC 
CATGTGGAGA AAGCCAGCCG AGTTTCTGCT GAAAACTTGC AAAAGAACCT AGATCAGATG 
AAGAAACAAA TTTCTGATGT GGAACGTGAT GTTCAGAATT TCCCAGCTGC CACAGATGAA 
AAAGACAAGT TTGTTGAAAA AATGACCAGC TTTGTGAAGG ATGCACAGGA ACAGTATAAC 
AAGCTGCGGA TGATGCATTC TAACATGGAG ACCCTCTATA AGGAGCTGGG CGAGTACTTC 
CTCTTTGACC CCAAGAAGTT GTCTGTTGAA GAATTTTTCA TGGATCTTCA CAATTTTCGG 
AATATGTTTT TGCAAGCAGT CAAGGAGAAC CAGAAGCGGC GGGAGACAGA AGAAAAGATG 
AGGCGAGCAA AACTAGCCAA GGAGAAGGCA GAGAAGGAGC GGCTAGAGAA GCAGCAGAAG 
AGAGAGCAAC TCATAGACAT GAATGCAGAG GGCGATGAGA CAGGTGTGAT GGACAGTCTT 
CTAGAAGCCC TGCAGTCAGG GGCAGCATTC CGACGGAAGA GAGGGCCCCG TCAAGCCAAC 
AGGAAGGCCG GGTGTGCAGT CACATCTCTG CTAGCTTCGG AGCTGACCAA GGATGATGCC 
ATGGCTGCTG TTCCTGCCAA GGTGTCCAAG AACAGTGAGA CATTCCCCAC AATCCTTGAG 
GAAGCCAAGG AGTTGGTTGG CCGTGCAAGC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	DIAPH1
Entrez Gene ID	1729
Full Name	diaphanous related formin 1
Synonyms	DFNA1,DIA1,DRF1,LFHL1,SCBMS,hDIA1
General protein information	Preferred Names diaphanous related formin 1 Names protein diaphanous homolog 1
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 5 Map Location: 5q31.3
Summary	This gene is a homolog of the Drosophila diaphanous gene, and has been linked to autosomal dominant, fully penetrant, nonsyndromic sensorineural progressive low-frequency hearing loss. Actin polymerization involves proteins known to interact with diaphanous protein in Drosophila and mouse. It has therefore been speculated that this gene may have a role in the regulation of actin polymerization in hair cells of the inner ear. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008].
Disorder MIM:	602121
Disorder Html:	Deafness, autosomal dominant 1, 124900 (3); Seizures, cortical blindness, microcephaly syndrome, 616632 (3)

Homo sapiens (human)	DIAPH1	NP_001073280.1
	DIAPH1	XP_001089232.2
Rattus norvegicus (Norway rat)	Diaph1	NP_001100863.2
Mus musculus (house mouse)	Diap1	NP_031884.1
Pan troglodytes (chimpanzee)	DIAPH1	XP_003310971.1
Drosophila melanogaster (fruit fly)	dia	NP_001246113.1
Canis lupus familiaris (dog)	DIAPH1	XP_005617363.1
Xenopus tropicalis (tropical clawed frog)	diaph1	XP_002940036.2

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

DIAPH1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

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