ORF » Species Summary » Homo sapiens » DHFR cDNA ORF clone

DHFR cDNA ORF clone, Homo sapiens(human)

The following DHFR gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DHFR cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu23976 NM_001290354.2
Latest version!		 Homo sapiens dihydrofolate reductase (DHFR), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK 		$49.50-$69.30
$99.00
OHu25053 NM_000791.4
Latest version!		 Homo sapiens dihydrofolate reductase (DHFR), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK 		$49.50-$69.30
$99.00
OHu23903 NM_001290357.2
Latest version!		 Homo sapiens dihydrofolate reductase (DHFR), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $69.30
$99.00
View Old Accession Versions >>
OHu23903 NM_001290357.1 Homo sapiens dihydrofolate reductase (DHFR), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $69.30
$99.00
OHu25053 NM_000791.3 Homo sapiens dihydrofolate reductase (DHFR), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK 		$49.50-$69.30
$99.00
OHu23976 NM_001290354.1 Homo sapiens dihydrofolate reductase (DHFR), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK 		$49.50-$69.30
$99.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    DHFR ( NM_001290354.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001277283.1 Homo sapiens dihydrofolate reductase (DHFR), transcript variant 2, mRNA.
    Price & Availability↑
    CloneID OHu23976
    Clone ID Related Accession (Same CDS sequence) NM_001290354.1 , NM_001290354.2
    Accession Version NM_001290354.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 408bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-03-11
    Organism Homo sapiens(human)
    Product dihydrofolate reductase isoform 2
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC010270.8, AC008434.5, AK293146.1 and AA489055.1. Transcript Variant: This variant (2) lacks an alternate exon in the 5' end compared to variant 1. This difference causes translation initiation at a downstream AUG and results in an isoform (2) with a shorter N-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK293146.1, BM787874.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: full length. 

    1
    61
    121
    181
    241
    301
    361
    ATGGGTAAGA AGACCTGGTT CTCCATTCCT GAGAAGAATC GACCTTTAAA GGGTAGAATT 
    AATTTAGTTC TCAGCAGAGA ACTCAAGGAA CCTCCACAAG GAGCTCATTT TCTTTCCAGA 
    AGTCTAGATG ATGCCTTAAA ACTTACTGAA CAACCAGAAT TAGCAAATAA AGTAGACATG 
    GTCTGGATAG TTGGTGGCAG TTCTGTTTAT AAGGAAGCCA TGAATCACCC AGGCCATCTT 
    AAACTATTTG TGACAAGGAT CATGCAAGAC TTTGAAAGTG ACACGTTTTT TCCAGAAATT 
    GATTTGGAGA AATATAAACT TCTGCCAGAA TACCCAGGTG TTCTCTCTGA TGTCCAGGAG 
    GAGAAAGGCA TTAAGTACAA ATTTGAAGTA TATGAGAAGA ATGATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001277283.1
    CDS599..1006
    Translation

    Target ORF information:

    RefSeq Version NM_001290354.1
    Organism Homo sapiens(human)
    Definition Homo sapiens dihydrofolate reductase (DHFR), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001290354.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    ATGGGTAAGA AGACCTGGTT CTCCATTCCT GAGAAGAATC GACCTTTAAA GGGTAGAATT 
    AATTTAGTTC TCAGCAGAGA ACTCAAGGAA CCTCCACAAG GAGCTCATTT TCTTTCCAGA 
    AGTCTAGATG ATGCCTTAAA ACTTACTGAA CAACCAGAAT TAGCAAATAA AGTAGACATG 
    GTCTGGATAG TTGGTGGCAG TTCTGTTTAT AAGGAAGCCA TGAATCACCC AGGCCATCTT 
    AAACTATTTG TGACAAGGAT CATGCAAGAC TTTGAAAGTG ACACGTTTTT TCCAGAAATT 
    GATTTGGAGA AATATAAACT TCTGCCAGAA TACCCAGGTG TTCTCTCTGA TGTCCAGGAG 
    GAGAAAGGCA TTAAGTACAA ATTTGAAGTA TATGAGAAGA ATGATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    DHFR ( NM_001290354.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001277283.1 Homo sapiens dihydrofolate reductase (DHFR), transcript variant 2, mRNA.
    Price & Availability↑
    CloneID OHu23976
    Clone ID Related Accession (Same CDS sequence) NM_001290354.1 , NM_001290354.2
    Accession Version NM_001290354.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 408bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-06-25
    Organism Homo sapiens(human)
    Product dihydrofolate reductase isoform 2
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC010270.8, AC008434.5, AK293146.1 and AA489055.1. On May 31, 2019 this sequence version replaced NM_001290354.1. Transcript Variant: This variant (2) lacks an alternate exon in the 5' end compared to variant 1. This difference causes translation initiation at a downstream AUG and results in an isoform (2) with a shorter N-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK293146.1, BM787874.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: full length. 

    1
    61
    121
    181
    241
    301
    361
    ATGGGTAAGA AGACCTGGTT CTCCATTCCT GAGAAGAATC GACCTTTAAA GGGTAGAATT 
    AATTTAGTTC TCAGCAGAGA ACTCAAGGAA CCTCCACAAG GAGCTCATTT TCTTTCCAGA 
    AGTCTAGATG ATGCCTTAAA ACTTACTGAA CAACCAGAAT TAGCAAATAA AGTAGACATG 
    GTCTGGATAG TTGGTGGCAG TTCTGTTTAT AAGGAAGCCA TGAATCACCC AGGCCATCTT 
    AAACTATTTG TGACAAGGAT CATGCAAGAC TTTGAAAGTG ACACGTTTTT TCCAGAAATT 
    GATTTGGAGA AATATAAACT TCTGCCAGAA TACCCAGGTG TTCTCTCTGA TGTCCAGGAG 
    GAGAAAGGCA TTAAGTACAA ATTTGAAGTA TATGAGAAGA ATGATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001277283.1
    CDS601..1008
    Translation

    Target ORF information:

    RefSeq Version NM_001290354.2
    Organism Homo sapiens(human)
    Definition Homo sapiens dihydrofolate reductase (DHFR), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001290354.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    ATGGGTAAGA AGACCTGGTT CTCCATTCCT GAGAAGAATC GACCTTTAAA GGGTAGAATT 
    AATTTAGTTC TCAGCAGAGA ACTCAAGGAA CCTCCACAAG GAGCTCATTT TCTTTCCAGA 
    AGTCTAGATG ATGCCTTAAA ACTTACTGAA CAACCAGAAT TAGCAAATAA AGTAGACATG 
    GTCTGGATAG TTGGTGGCAG TTCTGTTTAT AAGGAAGCCA TGAATCACCC AGGCCATCTT 
    AAACTATTTG TGACAAGGAT CATGCAAGAC TTTGAAAGTG ACACGTTTTT TCCAGAAATT 
    GATTTGGAGA AATATAAACT TCTGCCAGAA TACCCAGGTG TTCTCTCTGA TGTCCAGGAG 
    GAGAAAGGCA TTAAGTACAA ATTTGAAGTA TATGAGAAGA ATGATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    DHFR ( NM_000791.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_000782.1 Homo sapiens dihydrofolate reductase (DHFR), transcript variant 1, mRNA.
    Price & Availability↑
    CloneID OHu25053
    Clone ID Related Accession (Same CDS sequence) NM_000791.4 , NM_000791.3
    Accession Version NM_000791.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 564bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-21
    Organism Homo sapiens(human)
    Product dihydrofolate reductase isoform 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC010270.8, CB988944.1, AC008434.5, BC003584.1 and AA489055.1. This sequence is a reference standard in the RefSeqGene project. On Nov 23, 2018 this sequence version replaced NM_000791.3. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC000192.1, SRR1163658.305558.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145544 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: upstream AUG and CDS extension is not conserved MANE Ensembl match :: ENST00000439211.7/ ENSP00000396308.2 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    ATGGTTGGTT CGCTAAACTG CATCGTCGCT GTGTCCCAGA ACATGGGCAT CGGCAAGAAC 
    GGGGACCTGC CCTGGCCACC GCTCAGGAAT GAATTCAGAT ATTTCCAGAG AATGACCACA 
    ACCTCTTCAG TAGAAGGTAA ACAGAATCTG GTGATTATGG GTAAGAAGAC CTGGTTCTCC 
    ATTCCTGAGA AGAATCGACC TTTAAAGGGT AGAATTAATT TAGTTCTCAG CAGAGAACTC 
    AAGGAACCTC CACAAGGAGC TCATTTTCTT TCCAGAAGTC TAGATGATGC CTTAAAACTT 
    ACTGAACAAC CAGAATTAGC AAATAAAGTA GACATGGTCT GGATAGTTGG TGGCAGTTCT 
    GTTTATAAGG AAGCCATGAA TCACCCAGGC CATCTTAAAC TATTTGTGAC AAGGATCATG 
    CAAGACTTTG AAAGTGACAC GTTTTTTCCA GAAATTGATT TGGAGAAATA TAAACTTCTG 
    CCAGAATACC CAGGTGTTCT CTCTGATGTC CAGGAGGAGA AAGGCATTAA GTACAAATTT 
    GAAGTATATG AGAAGAATGA TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_000782.1
    CDS495..1058
    Translation

    Target ORF information:

    RefSeq Version NM_000791.4
    Organism Homo sapiens(human)
    Definition Homo sapiens dihydrofolate reductase (DHFR), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_000791.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    ATGGTTGGTT CGCTAAACTG CATCGTCGCT GTGTCCCAGA ACATGGGCAT CGGCAAGAAC 
    GGGGACCTGC CCTGGCCACC GCTCAGGAAT GAATTCAGAT ATTTCCAGAG AATGACCACA 
    ACCTCTTCAG TAGAAGGTAA ACAGAATCTG GTGATTATGG GTAAGAAGAC CTGGTTCTCC 
    ATTCCTGAGA AGAATCGACC TTTAAAGGGT AGAATTAATT TAGTTCTCAG CAGAGAACTC 
    AAGGAACCTC CACAAGGAGC TCATTTTCTT TCCAGAAGTC TAGATGATGC CTTAAAACTT 
    ACTGAACAAC CAGAATTAGC AAATAAAGTA GACATGGTCT GGATAGTTGG TGGCAGTTCT 
    GTTTATAAGG AAGCCATGAA TCACCCAGGC CATCTTAAAC TATTTGTGAC AAGGATCATG 
    CAAGACTTTG AAAGTGACAC GTTTTTTCCA GAAATTGATT TGGAGAAATA TAAACTTCTG 
    CCAGAATACC CAGGTGTTCT CTCTGATGTC CAGGAGGAGA AAGGCATTAA GTACAAATTT 
    GAAGTATATG AGAAGAATGA TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    DHFR ( NM_000791.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_000782.1 Homo sapiens dihydrofolate reductase (DHFR), transcript variant 1, mRNA.
    Price & Availability↑
    CloneID OHu25053
    Clone ID Related Accession (Same CDS sequence) NM_000791.4 , NM_000791.3
    Accession Version NM_000791.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 564bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-12-17
    Organism Homo sapiens(human)
    Product dihydrofolate reductase isoform 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CB988944.1, AC008434.5, BC003584.1 and AA489055.1. This sequence is a reference standard in the RefSeqGene project. On Jun 29, 2005 this sequence version replaced NM_000791.2. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC000192.1, SRR1163658.305558.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: upstream AUG and CDS extension is not conserved ##RefSeq-Attributes-END## COMPLETENESS: full length. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    ATGGTTGGTT CGCTAAACTG CATCGTCGCT GTGTCCCAGA ACATGGGCAT CGGCAAGAAC 
    GGGGACCTGC CCTGGCCACC GCTCAGGAAT GAATTCAGAT ATTTCCAGAG AATGACCACA 
    ACCTCTTCAG TAGAAGGTAA ACAGAATCTG GTGATTATGG GTAAGAAGAC CTGGTTCTCC 
    ATTCCTGAGA AGAATCGACC TTTAAAGGGT AGAATTAATT TAGTTCTCAG CAGAGAACTC 
    AAGGAACCTC CACAAGGAGC TCATTTTCTT TCCAGAAGTC TAGATGATGC CTTAAAACTT 
    ACTGAACAAC CAGAATTAGC AAATAAAGTA GACATGGTCT GGATAGTTGG TGGCAGTTCT 
    GTTTATAAGG AAGCCATGAA TCACCCAGGC CATCTTAAAC TATTTGTGAC AAGGATCATG 
    CAAGACTTTG AAAGTGACAC GTTTTTTCCA GAAATTGATT TGGAGAAATA TAAACTTCTG 
    CCAGAATACC CAGGTGTTCT CTCTGATGTC CAGGAGGAGA AAGGCATTAA GTACAAATTT 
    GAAGTATATG AGAAGAATGA TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_000782.1
    CDS493..1056
    Translation

    Target ORF information:

    RefSeq Version NM_000791.3
    Organism Homo sapiens(human)
    Definition Homo sapiens dihydrofolate reductase (DHFR), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_000791.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    ATGGTTGGTT CGCTAAACTG CATCGTCGCT GTGTCCCAGA ACATGGGCAT CGGCAAGAAC 
    GGGGACCTGC CCTGGCCACC GCTCAGGAAT GAATTCAGAT ATTTCCAGAG AATGACCACA 
    ACCTCTTCAG TAGAAGGTAA ACAGAATCTG GTGATTATGG GTAAGAAGAC CTGGTTCTCC 
    ATTCCTGAGA AGAATCGACC TTTAAAGGGT AGAATTAATT TAGTTCTCAG CAGAGAACTC 
    AAGGAACCTC CACAAGGAGC TCATTTTCTT TCCAGAAGTC TAGATGATGC CTTAAAACTT 
    ACTGAACAAC CAGAATTAGC AAATAAAGTA GACATGGTCT GGATAGTTGG TGGCAGTTCT 
    GTTTATAAGG AAGCCATGAA TCACCCAGGC CATCTTAAAC TATTTGTGAC AAGGATCATG 
    CAAGACTTTG AAAGTGACAC GTTTTTTCCA GAAATTGATT TGGAGAAATA TAAACTTCTG 
    CCAGAATACC CAGGTGTTCT CTCTGATGTC CAGGAGGAGA AAGGCATTAA GTACAAATTT 
    GAAGTATATG AGAAGAATGA TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    DHFR ( NM_001290357.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001277286.1 Homo sapiens dihydrofolate reductase (DHFR), transcript variant 3, mRNA.
    Price & Availability↑
    CloneID OHu23903
    Clone ID Related Accession (Same CDS sequence) NM_001290357.1 , NM_001290357.2
    Accession Version NM_001290357.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 390bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-03-11
    Organism Homo sapiens(human)
    Product dihydrofolate reductase isoform 3
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC010270.8, AC008434.5, AK297302.1 and AA489055.1. Transcript Variant: This variant (3) lacks an alternate exon in the 3' end compared to variant 1, that causes a frameshift. The resulting isoform (3) has a shorter and distinct C-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK297302.1, SRR1163658.380767.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: upstream AUG and CDS extension is not conserved ##RefSeq-Attributes-END## COMPLETENESS: full length. 

    1
    61
    121
    181
    241
    301
    361
    ATGGTTGGTT CGCTAAACTG CATCGTCGCT GTGTCCCAGA ACATGGGCAT CGGCAAGAAC 
    GGGGACCTGC CCTGGCCACC GCTCAGGAAT GAATTCAGAT ATTTCCAGAG AATGACCACA 
    ACCTCTTCAG TAGAAGGTAA ACAGAATCTG GTGATTATGG GTAAGAAGAC CTGGTTCTCC 
    ATTCCTGAGA AGAATCGACC TTTAAAGGGT AGAATTAATT TAGTTCTCAG CAGAGAACTC 
    AAGGAACCTC CACAAGGAGC TCATTTTCTT TCCAGAAGTC TAGATGATGC CTTAAAACTT 
    ACTGAACAAC CAGAATTAGC AAATAAAGTA GACATGGTCT GGATAGTTGG TGGCAGTTCT 
    GTTTATAAGA TACCCAGGTG TTCTCTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001277286.1
    CDS493..882
    Translation

    Target ORF information:

    RefSeq Version NM_001290357.1
    Organism Homo sapiens(human)
    Definition Homo sapiens dihydrofolate reductase (DHFR), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001290357.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    ATGGTTGGTT CGCTAAACTG CATCGTCGCT GTGTCCCAGA ACATGGGCAT CGGCAAGAAC 
    GGGGACCTGC CCTGGCCACC GCTCAGGAAT GAATTCAGAT ATTTCCAGAG AATGACCACA 
    ACCTCTTCAG TAGAAGGTAA ACAGAATCTG GTGATTATGG GTAAGAAGAC CTGGTTCTCC 
    ATTCCTGAGA AGAATCGACC TTTAAAGGGT AGAATTAATT TAGTTCTCAG CAGAGAACTC 
    AAGGAACCTC CACAAGGAGC TCATTTTCTT TCCAGAAGTC TAGATGATGC CTTAAAACTT 
    ACTGAACAAC CAGAATTAGC AAATAAAGTA GACATGGTCT GGATAGTTGG TGGCAGTTCT 
    GTTTATAAGA TACCCAGGTG TTCTCTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    DHFR ( NM_001290357.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001277286.1 Homo sapiens dihydrofolate reductase (DHFR), transcript variant 3, mRNA.
    Price & Availability↑
    CloneID OHu23903
    Clone ID Related Accession (Same CDS sequence) NM_001290357.1 , NM_001290357.2
    Accession Version NM_001290357.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 390bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-06-25
    Organism Homo sapiens(human)
    Product dihydrofolate reductase isoform 3
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC010270.8, AC008434.5, AK297302.1 and AA489055.1. On May 31, 2019 this sequence version replaced NM_001290357.1. Transcript Variant: This variant (3) lacks an alternate exon in the 3' end compared to variant 1, that causes a frameshift. The resulting isoform (3) has a shorter and distinct C-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK297302.1, SRR1163658.380767.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: upstream AUG and CDS extension is not conserved ##RefSeq-Attributes-END## COMPLETENESS: full length. 

    1
    61
    121
    181
    241
    301
    361
    ATGGTTGGTT CGCTAAACTG CATCGTCGCT GTGTCCCAGA ACATGGGCAT CGGCAAGAAC 
    GGGGACCTGC CCTGGCCACC GCTCAGGAAT GAATTCAGAT ATTTCCAGAG AATGACCACA 
    ACCTCTTCAG TAGAAGGTAA ACAGAATCTG GTGATTATGG GTAAGAAGAC CTGGTTCTCC 
    ATTCCTGAGA AGAATCGACC TTTAAAGGGT AGAATTAATT TAGTTCTCAG CAGAGAACTC 
    AAGGAACCTC CACAAGGAGC TCATTTTCTT TCCAGAAGTC TAGATGATGC CTTAAAACTT 
    ACTGAACAAC CAGAATTAGC AAATAAAGTA GACATGGTCT GGATAGTTGG TGGCAGTTCT 
    GTTTATAAGA TACCCAGGTG TTCTCTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001277286.1
    CDS495..884
    Translation

    Target ORF information:

    RefSeq Version NM_001290357.2
    Organism Homo sapiens(human)
    Definition Homo sapiens dihydrofolate reductase (DHFR), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001290357.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    ATGGTTGGTT CGCTAAACTG CATCGTCGCT GTGTCCCAGA ACATGGGCAT CGGCAAGAAC 
    GGGGACCTGC CCTGGCCACC GCTCAGGAAT GAATTCAGAT ATTTCCAGAG AATGACCACA 
    ACCTCTTCAG TAGAAGGTAA ACAGAATCTG GTGATTATGG GTAAGAAGAC CTGGTTCTCC 
    ATTCCTGAGA AGAATCGACC TTTAAAGGGT AGAATTAATT TAGTTCTCAG CAGAGAACTC 
    AAGGAACCTC CACAAGGAGC TCATTTTCTT TCCAGAAGTC TAGATGATGC CTTAAAACTT 
    ACTGAACAAC CAGAATTAGC AAATAAAGTA GACATGGTCT GGATAGTTGG TGGCAGTTCT 
    GTTTATAAGA TACCCAGGTG TTCTCTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

見積依頼

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