COCH cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	COCH
Entrez Gene ID	1690
Full Name	cochlin
Synonyms	COCH-5B2,COCH5B2,DFNA9
General protein information	Preferred Names cochlin Names cochlin coagulation factor C homolog, cochlin (Limulus polyphemus)
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 14 Map Location: 14q12
Summary	The protein encoded by this gene is highly conserved in human, mouse, and chicken, showing 94% and 79% amino acid identity of human to mouse and chicken sequences, respectively. Hybridization to this gene was detected in spindle-shaped cells located along nerve fibers between the auditory ganglion and sensory epithelium. These cells accompany neurites at the habenula perforata, the opening through which neurites extend to innervate hair cells. This and the pattern of expression of this gene in chicken inner ear paralleled the histologic findings of acidophilic deposits, consistent with mucopolysaccharide ground substance, in temporal bones from DFNA9 (autosomal dominant nonsyndromic sensorineural deafness 9) patients. Mutations that cause DFNA9 have been reported in this gene. Alternative splicing results in multiple transcript variants encoding the same protein. Additional splice variants encoding distinct isoforms have been described but their biological validities have not been demonstrated. [provided by RefSeq, Oct 2008].
Disorder MIM:	603196
Disorder Html:	Deafness, autosomal dominant 9, 601369 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_004086.2	NP_004077.1	cochlin isoform b precursor
XM_017021071.1	XP_016876560.1	cochlin isoform X1
NM_004086.3	NP_004077.1	cochlin isoform b precursor
NM_001135058.1	NP_001128530.1	cochlin isoform b precursor
XM_017021073.1	XP_016876562.1	cochlin isoform X2
NM_001347720.1	NP_001334649.1	cochlin isoform a precursor
XM_024449506.1	XP_024305274.1	cochlin isoform X2

Pathways from BioSystems

Homologies

	COCH	XP_001114797.1
Bos taurus (cattle)	COCH	NP_001071310.1
Gallus gallus (chicken)	COCH	NP_990268.1
Danio rerio (zebrafish)	coch	NP_001003823.1
Xenopus tropicalis (tropical clawed frog)	coch	XP_004917329.1
Pan troglodytes (chimpanzee)	COCH	XP_001171057.1
Canis lupus familiaris (dog)	COCH	XP_547762.4
Homo sapiens (human)	COCH	NP_001128530.1
Mus musculus (house mouse)	Coch	NP_031754.1

Gene Ontology
Bibliography

Related articles in PubMed

Impaired lymphoid extracellular matrix impedes antibacterial immunity in epidermolysis bullosa.
Nystr?m A, Bornert O, K?hl T, Gretzmeier C, Thriene K, Dengjel J, Pfister-Wartha A, Kiritsi D, Bruckner-Tuderman L
Proceedings of the National Academy of Sciences of the United States of America(2018 Jan)

Different Phenotypes of the Two Chinese Probands with the Same c.889G>A (p.C162Y) Mutation in COCH Gene Verify Different Mechanisms Underlying Autosomal Dominant Nonsyndromic Deafness 9.
Wang Q, Fei P, Gu H, Zhang Y, Ke X, Liu Y
PloS one12(1)e0170011(2017)

Massively Parallel Sequencing of a Chinese Family with DFNA9 Identified a Novel Missense Mutation in the LCCL Domain of COCH.
Gu X, Su W, Tang M, Guo L, Zhao L, Li H
Neural plasticity20165310192(2016)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

COCH expression is significantly downregulated in human masticatory mucosa during wound healing
Title: Human gingiva transcriptome during wound healing.

c.889G>A (p.C162Y) Mutation in COCH leads to vestibular dysfunction and autosomal dominant nonsyndromic deafness 9.The p.C162Y mutation causes either disruption of LCCL domain fragment cleavage or aggregation of mutant cochlin.
Title: Different Phenotypes of the Two Chinese Probands with the Same c.889G>A (p.C162Y) Mutation in COCH Gene Verify Different Mechanisms Underlying Autosomal Dominant Nonsyndromic Deafness 9.

Distinct vestibular phenotypes depending on the location of COCH mutations were demonstrated, and this study correlates a genotype of p.G38D in COCH to the phenotype of bilateral total vestibular loss, therefore expanding the vestibular phenotypic spectrum of DFNA9 to range from bilateral vestibular loss without episodic vertigo to MD-like features with devastating episodic vertigo
Title: Distinct vestibular phenotypes in DFNA9 families with COCH variants.

This study showed that Mendelian sensorineural hearing loss exhibits vestibular dysfunction, including DFNA9, DFNA11, DFNA15 and DFNA28.
Title: Genetics of vestibular disorders: pathophysiological insights.

A missense mutation in the LCCL domain of COCH was associated with autosomal dominant nonsyndromic sensorineural hearing loss in a Chinese family.
Title: Massively Parallel Sequencing of a Chinese Family with DFNA9 Identified a Novel Missense Mutation in the LCCL Domain of COCH.

The following COCH gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the COCH cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu27128	NM_001135058.1 Latest version!	Homo sapiens cochlin (COCH), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu27128	NM_004086.3 Latest version!	Homo sapiens cochlin (COCH), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu84818	XM_017021073.1 Latest version!	Homo sapiens cochlin (COCH), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OHu84817	NM_001347720.1 Latest version!	Homo sapiens cochlin (COCH), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OHu103870	XM_024449506.1 Latest version!	Homo sapiens cochlin (COCH), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OHu84817	XM_017021071.1 Latest version!	Homo sapiens cochlin (COCH), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
View Old Accession Versions >>
OHu27128	NM_004086.2	Homo sapiens cochlin (COCH), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

COCH ( NM_004086.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_004077.1 Homo sapiens cochlin (COCH), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu27128
Clone ID Related Accession (Same CDS sequence)	NM_004086.2 , NM_004086.3 , NM_001135058.1
Accession Version	NM_004086.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1653bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-03
Organism	Homo sapiens(human)
Product	cochlin isoform b precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AW629100.1 and AF006740.1. This sequence is a reference standard in the RefSeqGene project. On Sep 19, 2008 this sequence version replaced NM_004086.1. Transcript Variant: This variant (2) differs in the 5' UTR and uses an alternate in-frame splice junction in the 5' end compared to variant 3. The resulting isoform (b) has the same N- and C-termini but is shorter compared to isoform a. Variants 1 and 2 encode the same isoform (b). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF006740.1, SRR1660805.187027.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END##

ATGTCCGCAG CCTGGATCCC GGCTCTCGGC CTCGGTGTGT GTCTGCTGCT GCTGCCGGGG 
CCCGCGGGCA GCGAGGGAGC CGCTCCCATT GCTATCACAT GTTTTACCAG AGGCTTGGAC 
ATCAGGAAAG AGAAAGCAGA TGTCCTCTGC CCAGGGGGCT GCCCTCTTGA GGAATTCTCT 
GTGTATGGGA ACATAGTATA TGCTTCTGTA TCGAGCATAT GTGGGGCTGC TGTCCACAGG 
GGAGTAATCA GCAACTCAGG GGGACCTGTA CGAGTCTATA GCCTACCTGG TCGAGAAAAC 
TATTCCTCAG TAGATGCCAA TGGCATCCAG TCTCAAATGC TTTCTAGATG GTCTGCTTCT 
TTCACAGTAA CTAAAGGCAA AAGTAGTACA CAGGAGGCCA CAGGACAAGC AGTGTCCACA 
GCACATCCAC CAACAGGTAA ACGACTAAAG AAAACACCCG AGAAGAAAAC TGGCAATAAA 
GATTGTAAAG CAGACATTGC ATTTCTGATT GATGGAAGCT TTAATATTGG GCAGCGCCGA 
TTTAATTTAC AGAAGAATTT TGTTGGAAAA GTGGCTCTAA TGTTGGGAAT TGGAACAGAA 
GGACCACATG TGGGCCTTGT TCAAGCCAGT GAACATCCCA AAATAGAATT TTACTTGAAA 
AACTTTACAT CAGCCAAAGA TGTTTTGTTT GCCATAAAGG AAGTAGGTTT CAGAGGGGGT 
AATTCCAATA CAGGAAAAGC CTTGAAGCAT ACTGCTCAGA AATTCTTCAC GGTAGATGCT 
GGAGTAAGAA AAGGGATCCC CAAAGTGGTG GTGGTATTTA TTGATGGTTG GCCTTCTGAT 
GACATCGAGG AAGCAGGCAT TGTGGCCAGA GAGTTTGGTG TCAATGTATT TATAGTTTCT 
GTGGCCAAGC CTATCCCTGA AGAACTGGGG ATGGTTCAGG ATGTCACATT TGTTGACAAG 
GCTGTCTGTC GGAATAATGG CTTCTTCTCT TACCACATGC CCAACTGGTT TGGCACCACA 
AAATACGTAA AGCCTCTGGT ACAGAAGCTG TGCACTCATG AACAAATGAT GTGCAGCAAG 
ACCTGTTATA ACTCAGTGAA CATTGCCTTT CTAATTGATG GCTCCAGCAG TGTTGGAGAT 
AGCAATTTCC GCCTCATGCT TGAATTTGTT TCCAACATAG CCAAGACTTT TGAAATCTCG 
GACATTGGTG CCAAGATAGC TGCTGTACAG TTTACTTATG ATCAGCGCAC GGAGTTCAGT 
TTCACTGACT ATAGCACCAA AGAGAATGTC CTAGCTGTCA TCAGAAACAT CCGCTATATG 
AGTGGTGGAA CAGCTACTGG TGATGCCATT TCCTTCACTG TTAGAAATGT GTTTGGCCCT 
ATAAGGGAGA GCCCCAACAA GAACTTCCTA GTAATTGTCA CAGATGGGCA GTCCTATGAT 
GATGTCCAAG GCCCTGCAGC TGCTGCACAT GATGCAGGAA TCACTATCTT CTCTGTTGGT 
GTGGCTTGGG CACCTCTGGA TGACCTGAAA GATATGGCTT CTAAACCGAA GGAGTCTCAT 
GCTTTCTTCA CAAGAGAGTT CACAGGATTA GAACCAATTG TTTCTGATGT CATCAGAGGC 
ATTTGTAGAG ATTTCTTAGA ATCCCAGCAA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004077.1
CDS	81..1733
Translation	MSAAWIPALGLGVCLLLLPGPAGSEGAAPIAITCFTRGLDIRKEKADVLCPGGCPLEEFSVYGNIVYASVSSICGAAVHRGVISNSGGPVRVYSLPGRENYSSVDANGIQSQMLSRWSASFTVTKGKSSTQEATGQAVSTAHPPTGKRLKKTPEKKTGNKDCKADIAFLIDGSFNIGQRRFNLQKNFVGKVALMLGIGTEGPHVGLVQASEHPKIEFYLKNFTSAKDVLFAIKEVGFRGGNSNTGKALKHTAQKFFTVDAGVRKGIPKVVVVFIDGWPSDDIEEAGIVAREFGVNVFIVSVAKPIPEELGMVQDVTFVDKAVCRNNGFFSYHMPNWFGTTKYVKPLVQKLCTHEQMMCSKTCYNSVNIAFLIDGSSSVGDSNFRLMLEFVSNIAKTFEISDIGAKIAAVQFTYDQRTEFSFTDYSTKENVLAVIRNIRYMSGGTATGDAISFTVRNVFGPIRESPNKNFLVIVTDGQSYDDVQGPAAAAHDAGITIFSVGVAWAPLDDLKDMASKPKESHAFFTREFTGLEPIVSDVIRGICRDFLESQQ

Target ORF information:

RefSeq Version	NM_004086.2
Organism	Homo sapiens(human)
Definition	Homo sapiens cochlin (COCH), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004086.2

ORF Insert Sequence:

ATGTCCGCAG CCTGGATCCC GGCTCTCGGC CTCGGTGTGT GTCTGCTGCT GCTGCCGGGG 
CCCGCGGGCA GCGAGGGAGC CGCTCCCATT GCTATCACAT GTTTTACCAG AGGCTTGGAC 
ATCAGGAAAG AGAAAGCAGA TGTCCTCTGC CCAGGGGGCT GCCCTCTTGA GGAATTCTCT 
GTGTATGGGA ACATAGTATA TGCTTCTGTA TCGAGCATAT GTGGGGCTGC TGTCCACAGG 
GGAGTAATCA GCAACTCAGG GGGACCTGTA CGAGTCTATA GCCTACCTGG TCGAGAAAAC 
TATTCCTCAG TAGATGCCAA TGGCATCCAG TCTCAAATGC TTTCTAGATG GTCTGCTTCT 
TTCACAGTAA CTAAAGGCAA AAGTAGTACA CAGGAGGCCA CAGGACAAGC AGTGTCCACA 
GCACATCCAC CAACAGGTAA ACGACTAAAG AAAACACCCG AGAAGAAAAC TGGCAATAAA 
GATTGTAAAG CAGACATTGC ATTTCTGATT GATGGAAGCT TTAATATTGG GCAGCGCCGA 
TTTAATTTAC AGAAGAATTT TGTTGGAAAA GTGGCTCTAA TGTTGGGAAT TGGAACAGAA 
GGACCACATG TGGGCCTTGT TCAAGCCAGT GAACATCCCA AAATAGAATT TTACTTGAAA 
AACTTTACAT CAGCCAAAGA TGTTTTGTTT GCCATAAAGG AAGTAGGTTT CAGAGGGGGT 
AATTCCAATA CAGGAAAAGC CTTGAAGCAT ACTGCTCAGA AATTCTTCAC GGTAGATGCT 
GGAGTAAGAA AAGGGATCCC CAAAGTGGTG GTGGTATTTA TTGATGGTTG GCCTTCTGAT 
GACATCGAGG AAGCAGGCAT TGTGGCCAGA GAGTTTGGTG TCAATGTATT TATAGTTTCT 
GTGGCCAAGC CTATCCCTGA AGAACTGGGG ATGGTTCAGG ATGTCACATT TGTTGACAAG 
GCTGTCTGTC GGAATAATGG CTTCTTCTCT TACCACATGC CCAACTGGTT TGGCACCACA 
AAATACGTAA AGCCTCTGGT ACAGAAGCTG TGCACTCATG AACAAATGAT GTGCAGCAAG 
ACCTGTTATA ACTCAGTGAA CATTGCCTTT CTAATTGATG GCTCCAGCAG TGTTGGAGAT 
AGCAATTTCC GCCTCATGCT TGAATTTGTT TCCAACATAG CCAAGACTTT TGAAATCTCG 
GACATTGGTG CCAAGATAGC TGCTGTACAG TTTACTTATG ATCAGCGCAC GGAGTTCAGT 
TTCACTGACT ATAGCACCAA AGAGAATGTC CTAGCTGTCA TCAGAAACAT CCGCTATATG 
AGTGGTGGAA CAGCTACTGG TGATGCCATT TCCTTCACTG TTAGAAATGT GTTTGGCCCT 
ATAAGGGAGA GCCCCAACAA GAACTTCCTA GTAATTGTCA CAGATGGGCA GTCCTATGAT 
GATGTCCAAG GCCCTGCAGC TGCTGCACAT GATGCAGGAA TCACTATCTT CTCTGTTGGT 
GTGGCTTGGG CACCTCTGGA TGACCTGAAA GATATGGCTT CTAAACCGAA GGAGTCTCAT 
GCTTTCTTCA CAAGAGAGTT CACAGGATTA GAACCAATTG TTTCTGATGT CATCAGAGGC 
ATTTGTAGAG ATTTCTTAGA ATCCCAGCAA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COCH ( NM_004086.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_004077.1 Homo sapiens cochlin (COCH), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu27128
Clone ID Related Accession (Same CDS sequence)	NM_004086.2 , NM_004086.3 , NM_001135058.1
Accession Version	NM_004086.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1653bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-21
Organism	Homo sapiens(human)
Product	cochlin isoform b precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF006740.1 and AL049830.3. This sequence is a reference standard in the RefSeqGene project. On Nov 22, 2018 this sequence version replaced NM_004086.2. Transcript Variant: This variant (2) differs in the 5' UTR and uses an alternate in-frame splice junction in the 5' end compared to variant 3. The resulting isoform (b) has the same N- and C-termini but is shorter compared to isoform a. Variants 1 and 2 encode the same isoform (b). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF006740.1, SRR1660805.187027.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000396618.9/ ENSP00000379862.3 RefSeq Select criteria :: based on manual assertion, conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGTCCGCAG CCTGGATCCC GGCTCTCGGC CTCGGTGTGT GTCTGCTGCT GCTGCCGGGG 
CCCGCGGGCA GCGAGGGAGC CGCTCCCATT GCTATCACAT GTTTTACCAG AGGCTTGGAC 
ATCAGGAAAG AGAAAGCAGA TGTCCTCTGC CCAGGGGGCT GCCCTCTTGA GGAATTCTCT 
GTGTATGGGA ACATAGTATA TGCTTCTGTA TCGAGCATAT GTGGGGCTGC TGTCCACAGG 
GGAGTAATCA GCAACTCAGG GGGACCTGTA CGAGTCTATA GCCTACCTGG TCGAGAAAAC 
TATTCCTCAG TAGATGCCAA TGGCATCCAG TCTCAAATGC TTTCTAGATG GTCTGCTTCT 
TTCACAGTAA CTAAAGGCAA AAGTAGTACA CAGGAGGCCA CAGGACAAGC AGTGTCCACA 
GCACATCCAC CAACAGGTAA ACGACTAAAG AAAACACCCG AGAAGAAAAC TGGCAATAAA 
GATTGTAAAG CAGACATTGC ATTTCTGATT GATGGAAGCT TTAATATTGG GCAGCGCCGA 
TTTAATTTAC AGAAGAATTT TGTTGGAAAA GTGGCTCTAA TGTTGGGAAT TGGAACAGAA 
GGACCACATG TGGGCCTTGT TCAAGCCAGT GAACATCCCA AAATAGAATT TTACTTGAAA 
AACTTTACAT CAGCCAAAGA TGTTTTGTTT GCCATAAAGG AAGTAGGTTT CAGAGGGGGT 
AATTCCAATA CAGGAAAAGC CTTGAAGCAT ACTGCTCAGA AATTCTTCAC GGTAGATGCT 
GGAGTAAGAA AAGGGATCCC CAAAGTGGTG GTGGTATTTA TTGATGGTTG GCCTTCTGAT 
GACATCGAGG AAGCAGGCAT TGTGGCCAGA GAGTTTGGTG TCAATGTATT TATAGTTTCT 
GTGGCCAAGC CTATCCCTGA AGAACTGGGG ATGGTTCAGG ATGTCACATT TGTTGACAAG 
GCTGTCTGTC GGAATAATGG CTTCTTCTCT TACCACATGC CCAACTGGTT TGGCACCACA 
AAATACGTAA AGCCTCTGGT ACAGAAGCTG TGCACTCATG AACAAATGAT GTGCAGCAAG 
ACCTGTTATA ACTCAGTGAA CATTGCCTTT CTAATTGATG GCTCCAGCAG TGTTGGAGAT 
AGCAATTTCC GCCTCATGCT TGAATTTGTT TCCAACATAG CCAAGACTTT TGAAATCTCG 
GACATTGGTG CCAAGATAGC TGCTGTACAG TTTACTTATG ATCAGCGCAC GGAGTTCAGT 
TTCACTGACT ATAGCACCAA AGAGAATGTC CTAGCTGTCA TCAGAAACAT CCGCTATATG 
AGTGGTGGAA CAGCTACTGG TGATGCCATT TCCTTCACTG TTAGAAATGT GTTTGGCCCT 
ATAAGGGAGA GCCCCAACAA GAACTTCCTA GTAATTGTCA CAGATGGGCA GTCCTATGAT 
GATGTCCAAG GCCCTGCAGC TGCTGCACAT GATGCAGGAA TCACTATCTT CTCTGTTGGT 
GTGGCTTGGG CACCTCTGGA TGACCTGAAA GATATGGCTT CTAAACCGAA GGAGTCTCAT 
GCTTTCTTCA CAAGAGAGTT CACAGGATTA GAACCAATTG TTTCTGATGT CATCAGAGGC 
ATTTGTAGAG ATTTCTTAGA ATCCCAGCAA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004077.1
CDS	57..1709
Translation	MSAAWIPALGLGVCLLLLPGPAGSEGAAPIAITCFTRGLDIRKEKADVLCPGGCPLEEFSVYGNIVYASVSSICGAAVHRGVISNSGGPVRVYSLPGRENYSSVDANGIQSQMLSRWSASFTVTKGKSSTQEATGQAVSTAHPPTGKRLKKTPEKKTGNKDCKADIAFLIDGSFNIGQRRFNLQKNFVGKVALMLGIGTEGPHVGLVQASEHPKIEFYLKNFTSAKDVLFAIKEVGFRGGNSNTGKALKHTAQKFFTVDAGVRKGIPKVVVVFIDGWPSDDIEEAGIVAREFGVNVFIVSVAKPIPEELGMVQDVTFVDKAVCRNNGFFSYHMPNWFGTTKYVKPLVQKLCTHEQMMCSKTCYNSVNIAFLIDGSSSVGDSNFRLMLEFVSNIAKTFEISDIGAKIAAVQFTYDQRTEFSFTDYSTKENVLAVIRNIRYMSGGTATGDAISFTVRNVFGPIRESPNKNFLVIVTDGQSYDDVQGPAAAAHDAGITIFSVGVAWAPLDDLKDMASKPKESHAFFTREFTGLEPIVSDVIRGICRDFLESQQ

Target ORF information:

RefSeq Version	NM_004086.3
Organism	Homo sapiens(human)
Definition	Homo sapiens cochlin (COCH), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004086.3

ORF Insert Sequence:

ATGTCCGCAG CCTGGATCCC GGCTCTCGGC CTCGGTGTGT GTCTGCTGCT GCTGCCGGGG 
CCCGCGGGCA GCGAGGGAGC CGCTCCCATT GCTATCACAT GTTTTACCAG AGGCTTGGAC 
ATCAGGAAAG AGAAAGCAGA TGTCCTCTGC CCAGGGGGCT GCCCTCTTGA GGAATTCTCT 
GTGTATGGGA ACATAGTATA TGCTTCTGTA TCGAGCATAT GTGGGGCTGC TGTCCACAGG 
GGAGTAATCA GCAACTCAGG GGGACCTGTA CGAGTCTATA GCCTACCTGG TCGAGAAAAC 
TATTCCTCAG TAGATGCCAA TGGCATCCAG TCTCAAATGC TTTCTAGATG GTCTGCTTCT 
TTCACAGTAA CTAAAGGCAA AAGTAGTACA CAGGAGGCCA CAGGACAAGC AGTGTCCACA 
GCACATCCAC CAACAGGTAA ACGACTAAAG AAAACACCCG AGAAGAAAAC TGGCAATAAA 
GATTGTAAAG CAGACATTGC ATTTCTGATT GATGGAAGCT TTAATATTGG GCAGCGCCGA 
TTTAATTTAC AGAAGAATTT TGTTGGAAAA GTGGCTCTAA TGTTGGGAAT TGGAACAGAA 
GGACCACATG TGGGCCTTGT TCAAGCCAGT GAACATCCCA AAATAGAATT TTACTTGAAA 
AACTTTACAT CAGCCAAAGA TGTTTTGTTT GCCATAAAGG AAGTAGGTTT CAGAGGGGGT 
AATTCCAATA CAGGAAAAGC CTTGAAGCAT ACTGCTCAGA AATTCTTCAC GGTAGATGCT 
GGAGTAAGAA AAGGGATCCC CAAAGTGGTG GTGGTATTTA TTGATGGTTG GCCTTCTGAT 
GACATCGAGG AAGCAGGCAT TGTGGCCAGA GAGTTTGGTG TCAATGTATT TATAGTTTCT 
GTGGCCAAGC CTATCCCTGA AGAACTGGGG ATGGTTCAGG ATGTCACATT TGTTGACAAG 
GCTGTCTGTC GGAATAATGG CTTCTTCTCT TACCACATGC CCAACTGGTT TGGCACCACA 
AAATACGTAA AGCCTCTGGT ACAGAAGCTG TGCACTCATG AACAAATGAT GTGCAGCAAG 
ACCTGTTATA ACTCAGTGAA CATTGCCTTT CTAATTGATG GCTCCAGCAG TGTTGGAGAT 
AGCAATTTCC GCCTCATGCT TGAATTTGTT TCCAACATAG CCAAGACTTT TGAAATCTCG 
GACATTGGTG CCAAGATAGC TGCTGTACAG TTTACTTATG ATCAGCGCAC GGAGTTCAGT 
TTCACTGACT ATAGCACCAA AGAGAATGTC CTAGCTGTCA TCAGAAACAT CCGCTATATG 
AGTGGTGGAA CAGCTACTGG TGATGCCATT TCCTTCACTG TTAGAAATGT GTTTGGCCCT 
ATAAGGGAGA GCCCCAACAA GAACTTCCTA GTAATTGTCA CAGATGGGCA GTCCTATGAT 
GATGTCCAAG GCCCTGCAGC TGCTGCACAT GATGCAGGAA TCACTATCTT CTCTGTTGGT 
GTGGCTTGGG CACCTCTGGA TGACCTGAAA GATATGGCTT CTAAACCGAA GGAGTCTCAT 
GCTTTCTTCA CAAGAGAGTT CACAGGATTA GAACCAATTG TTTCTGATGT CATCAGAGGC 
ATTTGTAGAG ATTTCTTAGA ATCCCAGCAA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COCH ( NM_001135058.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001128530.1 Homo sapiens cochlin (COCH), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu27128
Clone ID Related Accession (Same CDS sequence)	NM_004086.2 , NM_004086.3 , NM_001135058.1
Accession Version	NM_001135058.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1653bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-17
Organism	Homo sapiens(human)
Product	cochlin isoform b precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AW629100.1, AY358900.1 and AF006740.1. Transcript Variant: This variant (1) uses an alternate in-frame splice junction in the 5' end compared to variant 3. The resulting isoform (b) has the same N- and C-termini but is shorter compared to isoform a. Variants 1 and 2 encode the same isoform (b). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.134292.1, SRR1660805.198825.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END##

ATGTCCGCAG CCTGGATCCC GGCTCTCGGC CTCGGTGTGT GTCTGCTGCT GCTGCCGGGG 
CCCGCGGGCA GCGAGGGAGC CGCTCCCATT GCTATCACAT GTTTTACCAG AGGCTTGGAC 
ATCAGGAAAG AGAAAGCAGA TGTCCTCTGC CCAGGGGGCT GCCCTCTTGA GGAATTCTCT 
GTGTATGGGA ACATAGTATA TGCTTCTGTA TCGAGCATAT GTGGGGCTGC TGTCCACAGG 
GGAGTAATCA GCAACTCAGG GGGACCTGTA CGAGTCTATA GCCTACCTGG TCGAGAAAAC 
TATTCCTCAG TAGATGCCAA TGGCATCCAG TCTCAAATGC TTTCTAGATG GTCTGCTTCT 
TTCACAGTAA CTAAAGGCAA AAGTAGTACA CAGGAGGCCA CAGGACAAGC AGTGTCCACA 
GCACATCCAC CAACAGGTAA ACGACTAAAG AAAACACCCG AGAAGAAAAC TGGCAATAAA 
GATTGTAAAG CAGACATTGC ATTTCTGATT GATGGAAGCT TTAATATTGG GCAGCGCCGA 
TTTAATTTAC AGAAGAATTT TGTTGGAAAA GTGGCTCTAA TGTTGGGAAT TGGAACAGAA 
GGACCACATG TGGGCCTTGT TCAAGCCAGT GAACATCCCA AAATAGAATT TTACTTGAAA 
AACTTTACAT CAGCCAAAGA TGTTTTGTTT GCCATAAAGG AAGTAGGTTT CAGAGGGGGT 
AATTCCAATA CAGGAAAAGC CTTGAAGCAT ACTGCTCAGA AATTCTTCAC GGTAGATGCT 
GGAGTAAGAA AAGGGATCCC CAAAGTGGTG GTGGTATTTA TTGATGGTTG GCCTTCTGAT 
GACATCGAGG AAGCAGGCAT TGTGGCCAGA GAGTTTGGTG TCAATGTATT TATAGTTTCT 
GTGGCCAAGC CTATCCCTGA AGAACTGGGG ATGGTTCAGG ATGTCACATT TGTTGACAAG 
GCTGTCTGTC GGAATAATGG CTTCTTCTCT TACCACATGC CCAACTGGTT TGGCACCACA 
AAATACGTAA AGCCTCTGGT ACAGAAGCTG TGCACTCATG AACAAATGAT GTGCAGCAAG 
ACCTGTTATA ACTCAGTGAA CATTGCCTTT CTAATTGATG GCTCCAGCAG TGTTGGAGAT 
AGCAATTTCC GCCTCATGCT TGAATTTGTT TCCAACATAG CCAAGACTTT TGAAATCTCG 
GACATTGGTG CCAAGATAGC TGCTGTACAG TTTACTTATG ATCAGCGCAC GGAGTTCAGT 
TTCACTGACT ATAGCACCAA AGAGAATGTC CTAGCTGTCA TCAGAAACAT CCGCTATATG 
AGTGGTGGAA CAGCTACTGG TGATGCCATT TCCTTCACTG TTAGAAATGT GTTTGGCCCT 
ATAAGGGAGA GCCCCAACAA GAACTTCCTA GTAATTGTCA CAGATGGGCA GTCCTATGAT 
GATGTCCAAG GCCCTGCAGC TGCTGCACAT GATGCAGGAA TCACTATCTT CTCTGTTGGT 
GTGGCTTGGG CACCTCTGGA TGACCTGAAA GATATGGCTT CTAAACCGAA GGAGTCTCAT 
GCTTTCTTCA CAAGAGAGTT CACAGGATTA GAACCAATTG TTTCTGATGT CATCAGAGGC 
ATTTGTAGAG ATTTCTTAGA ATCCCAGCAA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001128530.1
CDS	405..2057
Translation	MSAAWIPALGLGVCLLLLPGPAGSEGAAPIAITCFTRGLDIRKEKADVLCPGGCPLEEFSVYGNIVYASVSSICGAAVHRGVISNSGGPVRVYSLPGRENYSSVDANGIQSQMLSRWSASFTVTKGKSSTQEATGQAVSTAHPPTGKRLKKTPEKKTGNKDCKADIAFLIDGSFNIGQRRFNLQKNFVGKVALMLGIGTEGPHVGLVQASEHPKIEFYLKNFTSAKDVLFAIKEVGFRGGNSNTGKALKHTAQKFFTVDAGVRKGIPKVVVVFIDGWPSDDIEEAGIVAREFGVNVFIVSVAKPIPEELGMVQDVTFVDKAVCRNNGFFSYHMPNWFGTTKYVKPLVQKLCTHEQMMCSKTCYNSVNIAFLIDGSSSVGDSNFRLMLEFVSNIAKTFEISDIGAKIAAVQFTYDQRTEFSFTDYSTKENVLAVIRNIRYMSGGTATGDAISFTVRNVFGPIRESPNKNFLVIVTDGQSYDDVQGPAAAAHDAGITIFSVGVAWAPLDDLKDMASKPKESHAFFTREFTGLEPIVSDVIRGICRDFLESQQ

Target ORF information:

RefSeq Version	NM_001135058.1
Organism	Homo sapiens(human)
Definition	Homo sapiens cochlin (COCH), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001135058.1

ORF Insert Sequence:

ATGTCCGCAG CCTGGATCCC GGCTCTCGGC CTCGGTGTGT GTCTGCTGCT GCTGCCGGGG 
CCCGCGGGCA GCGAGGGAGC CGCTCCCATT GCTATCACAT GTTTTACCAG AGGCTTGGAC 
ATCAGGAAAG AGAAAGCAGA TGTCCTCTGC CCAGGGGGCT GCCCTCTTGA GGAATTCTCT 
GTGTATGGGA ACATAGTATA TGCTTCTGTA TCGAGCATAT GTGGGGCTGC TGTCCACAGG 
GGAGTAATCA GCAACTCAGG GGGACCTGTA CGAGTCTATA GCCTACCTGG TCGAGAAAAC 
TATTCCTCAG TAGATGCCAA TGGCATCCAG TCTCAAATGC TTTCTAGATG GTCTGCTTCT 
TTCACAGTAA CTAAAGGCAA AAGTAGTACA CAGGAGGCCA CAGGACAAGC AGTGTCCACA 
GCACATCCAC CAACAGGTAA ACGACTAAAG AAAACACCCG AGAAGAAAAC TGGCAATAAA 
GATTGTAAAG CAGACATTGC ATTTCTGATT GATGGAAGCT TTAATATTGG GCAGCGCCGA 
TTTAATTTAC AGAAGAATTT TGTTGGAAAA GTGGCTCTAA TGTTGGGAAT TGGAACAGAA 
GGACCACATG TGGGCCTTGT TCAAGCCAGT GAACATCCCA AAATAGAATT TTACTTGAAA 
AACTTTACAT CAGCCAAAGA TGTTTTGTTT GCCATAAAGG AAGTAGGTTT CAGAGGGGGT 
AATTCCAATA CAGGAAAAGC CTTGAAGCAT ACTGCTCAGA AATTCTTCAC GGTAGATGCT 
GGAGTAAGAA AAGGGATCCC CAAAGTGGTG GTGGTATTTA TTGATGGTTG GCCTTCTGAT 
GACATCGAGG AAGCAGGCAT TGTGGCCAGA GAGTTTGGTG TCAATGTATT TATAGTTTCT 
GTGGCCAAGC CTATCCCTGA AGAACTGGGG ATGGTTCAGG ATGTCACATT TGTTGACAAG 
GCTGTCTGTC GGAATAATGG CTTCTTCTCT TACCACATGC CCAACTGGTT TGGCACCACA 
AAATACGTAA AGCCTCTGGT ACAGAAGCTG TGCACTCATG AACAAATGAT GTGCAGCAAG 
ACCTGTTATA ACTCAGTGAA CATTGCCTTT CTAATTGATG GCTCCAGCAG TGTTGGAGAT 
AGCAATTTCC GCCTCATGCT TGAATTTGTT TCCAACATAG CCAAGACTTT TGAAATCTCG 
GACATTGGTG CCAAGATAGC TGCTGTACAG TTTACTTATG ATCAGCGCAC GGAGTTCAGT 
TTCACTGACT ATAGCACCAA AGAGAATGTC CTAGCTGTCA TCAGAAACAT CCGCTATATG 
AGTGGTGGAA CAGCTACTGG TGATGCCATT TCCTTCACTG TTAGAAATGT GTTTGGCCCT 
ATAAGGGAGA GCCCCAACAA GAACTTCCTA GTAATTGTCA CAGATGGGCA GTCCTATGAT 
GATGTCCAAG GCCCTGCAGC TGCTGCACAT GATGCAGGAA TCACTATCTT CTCTGTTGGT 
GTGGCTTGGG CACCTCTGGA TGACCTGAAA GATATGGCTT CTAAACCGAA GGAGTCTCAT 
GCTTTCTTCA CAAGAGAGTT CACAGGATTA GAACCAATTG TTTCTGATGT CATCAGAGGC 
ATTTGTAGAG ATTTCTTAGA ATCCCAGCAA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COCH ( XM_017021073.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016876562.1 Homo sapiens cochlin (COCH), transcript variant X3, mRNA.

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CloneID	OHu84818
Clone ID Related Accession (Same CDS sequence)	XM_017021073.1
Accession Version	XM_017021073.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1023bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	cochlin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000014.9) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTAAAAA GAAGTGAAAA TCAACAGACT TATCTAATGA ATGCAGATGT GGCAGAAAGA 
ATGAGTAGCA CTACCGTTGA CTCTGAAGAG AGACTTCTTA GAGGAAAAGC CTTGAAGCAT 
ACTGCTCAGA AATTCTTCAC GGTAGATGCT GGAGTAAGAA AAGGGATCCC CAAAGTGGTG 
GTGGTATTTA TTGATGGTTG GCCTTCTGAT GACATCGAGG AAGCAGGCAT TGTGGCCAGA 
GAGTTTGGTG TCAATGTATT TATAGTTTCT GTGGCCAAGC CTATCCCTGA AGAACTGGGG 
ATGGTTCAGG ATGTCACATT TGTTGACAAG GCTGTCTGTC GGAATAATGG CTTCTTCTCT 
TACCACATGC CCAACTGGTT TGGCACCACA AAATACGTAA AGCCTCTGGT ACAGAAGCTG 
TGCACTCATG AACAAATGAT GTGCAGCAAG ACCTGTTATA ACTCAGTGAA CATTGCCTTT 
CTAATTGATG GCTCCAGCAG TGTTGGAGAT AGCAATTTCC GCCTCATGCT TGAATTTGTT 
TCCAACATAG CCAAGACTTT TGAAATCTCG GACATTGGTG CCAAGATAGC TGCTGTACAG 
TTTACTTATG ATCAGCGCAC GGAGTTCAGT TTCACTGACT ATAGCACCAA AGAGAATGTC 
CTAGCTGTCA TCAGAAACAT CCGCTATATG AGTGGTGGAA CAGCTACTGG TGATGCCATT 
TCCTTCACTG TTAGAAATGT GTTTGGCCCT ATAAGGGAGA GCCCCAACAA GAACTTCCTA 
GTAATTGTCA CAGATGGGCA GTCCTATGAT GATGTCCAAG GCCCTGCAGC TGCTGCACAT 
GATGCAGGAA TCACTATCTT CTCTGTTGGT GTGGCTTGGG CACCTCTGGA TGACCTGAAA 
GATATGGCTT CTAAACCGAA GGAGTCTCAT GCTTTCTTCA CAAGAGAGTT CACAGGATTA 
GAACCAATTG TTTCTGATGT CATCAGAGGC ATTTGTAGAG ATTTCTTAGA ATCCCAGCAA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016876562.1
CDS	93..1115
Translation	MLKRSENQQTYLMNADVAERMSSTTVDSEERLLRGKALKHTAQKFFTVDAGVRKGIPKVVVVFIDGWPSDDIEEAGIVAREFGVNVFIVSVAKPIPEELGMVQDVTFVDKAVCRNNGFFSYHMPNWFGTTKYVKPLVQKLCTHEQMMCSKTCYNSVNIAFLIDGSSSVGDSNFRLMLEFVSNIAKTFEISDIGAKIAAVQFTYDQRTEFSFTDYSTKENVLAVIRNIRYMSGGTATGDAISFTVRNVFGPIRESPNKNFLVIVTDGQSYDDVQGPAAAAHDAGITIFSVGVAWAPLDDLKDMASKPKESHAFFTREFTGLEPIVSDVIRGICRDFLESQQ

Target ORF information:

RefSeq Version	XM_017021073.1
Organism	Homo sapiens(human)
Definition	Homo sapiens cochlin (COCH), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017021073.1

ORF Insert Sequence:

ATGCTAAAAA GAAGTGAAAA TCAACAGACT TATCTAATGA ATGCAGATGT GGCAGAAAGA 
ATGAGTAGCA CTACCGTTGA CTCTGAAGAG AGACTTCTTA GAGGAAAAGC CTTGAAGCAT 
ACTGCTCAGA AATTCTTCAC GGTAGATGCT GGAGTAAGAA AAGGGATCCC CAAAGTGGTG 
GTGGTATTTA TTGATGGTTG GCCTTCTGAT GACATCGAGG AAGCAGGCAT TGTGGCCAGA 
GAGTTTGGTG TCAATGTATT TATAGTTTCT GTGGCCAAGC CTATCCCTGA AGAACTGGGG 
ATGGTTCAGG ATGTCACATT TGTTGACAAG GCTGTCTGTC GGAATAATGG CTTCTTCTCT 
TACCACATGC CCAACTGGTT TGGCACCACA AAATACGTAA AGCCTCTGGT ACAGAAGCTG 
TGCACTCATG AACAAATGAT GTGCAGCAAG ACCTGTTATA ACTCAGTGAA CATTGCCTTT 
CTAATTGATG GCTCCAGCAG TGTTGGAGAT AGCAATTTCC GCCTCATGCT TGAATTTGTT 
TCCAACATAG CCAAGACTTT TGAAATCTCG GACATTGGTG CCAAGATAGC TGCTGTACAG 
TTTACTTATG ATCAGCGCAC GGAGTTCAGT TTCACTGACT ATAGCACCAA AGAGAATGTC 
CTAGCTGTCA TCAGAAACAT CCGCTATATG AGTGGTGGAA CAGCTACTGG TGATGCCATT 
TCCTTCACTG TTAGAAATGT GTTTGGCCCT ATAAGGGAGA GCCCCAACAA GAACTTCCTA 
GTAATTGTCA CAGATGGGCA GTCCTATGAT GATGTCCAAG GCCCTGCAGC TGCTGCACAT 
GATGCAGGAA TCACTATCTT CTCTGTTGGT GTGGCTTGGG CACCTCTGGA TGACCTGAAA 
GATATGGCTT CTAAACCGAA GGAGTCTCAT GCTTTCTTCA CAAGAGAGTT CACAGGATTA 
GAACCAATTG TTTCTGATGT CATCAGAGGC ATTTGTAGAG ATTTCTTAGA ATCCCAGCAA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COCH ( NM_001347720.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001334649.1 Homo sapiens cochlin (COCH), transcript variant 3, mRNA.

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CloneID	OHu84817
Clone ID Related Accession (Same CDS sequence)	XM_017021071.1 , NM_001347720.1
Accession Version	NM_001347720.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1848bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-17
Organism	Homo sapiens(human)
Product	cochlin isoform a precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL049830.3. On Dec 9, 2016 this sequence version replaced XM_017021072.1. Transcript Variant: This variant (3) represents the longest transcript and encodes the longer isoform (a). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.180899.1, SRR1803611.32571.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2145240 [ECO:0000348] ##Evidence-Data-END##

ATGTCCGCAG CCTGGATCCC GGCTCTCGGC CTCGGTGTGT GTCTGCTGCT GCTGCCGGGG 
CCCGCGGGCA GCGAGGGAGC CGGTGAGTGG GGGAGCTGGG GTGCGTCCAG GCGGTCGCAG 
GGGCTGAGCA CCAGCGGGTA CAAGCGGGAC TCAGATCCAG CCCCTTGGGC TTCAGCCCTA 
CCGCCTGAGG AGGAAGGCGC GAAGGTTGAG CCGCCGCGTG GCGCGCCCGC GTTAACCCCT 
GCAGCCGATC TGCTCCTGCT CACCTGTTTC TCCCATGCTC CCATTGCTAT CACATGTTTT 
ACCAGAGGCT TGGACATCAG GAAAGAGAAA GCAGATGTCC TCTGCCCAGG GGGCTGCCCT 
CTTGAGGAAT TCTCTGTGTA TGGGAACATA GTATATGCTT CTGTATCGAG CATATGTGGG 
GCTGCTGTCC ACAGGGGAGT AATCAGCAAC TCAGGGGGAC CTGTACGAGT CTATAGCCTA 
CCTGGTCGAG AAAACTATTC CTCAGTAGAT GCCAATGGCA TCCAGTCTCA AATGCTTTCT 
AGATGGTCTG CTTCTTTCAC AGTAACTAAA GGCAAAAGTA GTACACAGGA GGCCACAGGA 
CAAGCAGTGT CCACAGCACA TCCACCAACA GGTAAACGAC TAAAGAAAAC ACCCGAGAAG 
AAAACTGGCA ATAAAGATTG TAAAGCAGAC ATTGCATTTC TGATTGATGG AAGCTTTAAT 
ATTGGGCAGC GCCGATTTAA TTTACAGAAG AATTTTGTTG GAAAAGTGGC TCTAATGTTG 
GGAATTGGAA CAGAAGGACC ACATGTGGGC CTTGTTCAAG CCAGTGAACA TCCCAAAATA 
GAATTTTACT TGAAAAACTT TACATCAGCC AAAGATGTTT TGTTTGCCAT AAAGGAAGTA 
GGTTTCAGAG GGGGTAATTC CAATACAGGA AAAGCCTTGA AGCATACTGC TCAGAAATTC 
TTCACGGTAG ATGCTGGAGT AAGAAAAGGG ATCCCCAAAG TGGTGGTGGT ATTTATTGAT 
GGTTGGCCTT CTGATGACAT CGAGGAAGCA GGCATTGTGG CCAGAGAGTT TGGTGTCAAT 
GTATTTATAG TTTCTGTGGC CAAGCCTATC CCTGAAGAAC TGGGGATGGT TCAGGATGTC 
ACATTTGTTG ACAAGGCTGT CTGTCGGAAT AATGGCTTCT TCTCTTACCA CATGCCCAAC 
TGGTTTGGCA CCACAAAATA CGTAAAGCCT CTGGTACAGA AGCTGTGCAC TCATGAACAA 
ATGATGTGCA GCAAGACCTG TTATAACTCA GTGAACATTG CCTTTCTAAT TGATGGCTCC 
AGCAGTGTTG GAGATAGCAA TTTCCGCCTC ATGCTTGAAT TTGTTTCCAA CATAGCCAAG 
ACTTTTGAAA TCTCGGACAT TGGTGCCAAG ATAGCTGCTG TACAGTTTAC TTATGATCAG 
CGCACGGAGT TCAGTTTCAC TGACTATAGC ACCAAAGAGA ATGTCCTAGC TGTCATCAGA 
AACATCCGCT ATATGAGTGG TGGAACAGCT ACTGGTGATG CCATTTCCTT CACTGTTAGA 
AATGTGTTTG GCCCTATAAG GGAGAGCCCC AACAAGAACT TCCTAGTAAT TGTCACAGAT 
GGGCAGTCCT ATGATGATGT CCAAGGCCCT GCAGCTGCTG CACATGATGC AGGAATCACT 
ATCTTCTCTG TTGGTGTGGC TTGGGCACCT CTGGATGACC TGAAAGATAT GGCTTCTAAA 
CCGAAGGAGT CTCATGCTTT CTTCACAAGA GAGTTCACAG GATTAGAACC AATTGTTTCT 
GATGTCATCA GAGGCATTTG TAGAGATTTC TTAGAATCCC AGCAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001334649.1
CDS	405..2252
Translation	MSAAWIPALGLGVCLLLLPGPAGSEGAGEWGSWGASRRSQGLSTSGYKRDSDPAPWASALPPEEEGAKVEPPRGAPALTPAADLLLLTCFSHAPIAITCFTRGLDIRKEKADVLCPGGCPLEEFSVYGNIVYASVSSICGAAVHRGVISNSGGPVRVYSLPGRENYSSVDANGIQSQMLSRWSASFTVTKGKSSTQEATGQAVSTAHPPTGKRLKKTPEKKTGNKDCKADIAFLIDGSFNIGQRRFNLQKNFVGKVALMLGIGTEGPHVGLVQASEHPKIEFYLKNFTSAKDVLFAIKEVGFRGGNSNTGKALKHTAQKFFTVDAGVRKGIPKVVVVFIDGWPSDDIEEAGIVAREFGVNVFIVSVAKPIPEELGMVQDVTFVDKAVCRNNGFFSYHMPNWFGTTKYVKPLVQKLCTHEQMMCSKTCYNSVNIAFLIDGSSSVGDSNFRLMLEFVSNIAKTFEISDIGAKIAAVQFTYDQRTEFSFTDYSTKENVLAVIRNIRYMSGGTATGDAISFTVRNVFGPIRESPNKNFLVIVTDGQSYDDVQGPAAAAHDAGITIFSVGVAWAPLDDLKDMASKPKESHAFFTREFTGLEPIVSDVIRGICRDFLESQQ

Target ORF information:

RefSeq Version	NM_001347720.1
Organism	Homo sapiens(human)
Definition	Homo sapiens cochlin (COCH), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001347720.1

ORF Insert Sequence:

ATGTCCGCAG CCTGGATCCC GGCTCTCGGC CTCGGTGTGT GTCTGCTGCT GCTGCCGGGG 
CCCGCGGGCA GCGAGGGAGC CGGTGAGTGG GGGAGCTGGG GTGCGTCCAG GCGGTCGCAG 
GGGCTGAGCA CCAGCGGGTA CAAGCGGGAC TCAGATCCAG CCCCTTGGGC TTCAGCCCTA 
CCGCCTGAGG AGGAAGGCGC GAAGGTTGAG CCGCCGCGTG GCGCGCCCGC GTTAACCCCT 
GCAGCCGATC TGCTCCTGCT CACCTGTTTC TCCCATGCTC CCATTGCTAT CACATGTTTT 
ACCAGAGGCT TGGACATCAG GAAAGAGAAA GCAGATGTCC TCTGCCCAGG GGGCTGCCCT 
CTTGAGGAAT TCTCTGTGTA TGGGAACATA GTATATGCTT CTGTATCGAG CATATGTGGG 
GCTGCTGTCC ACAGGGGAGT AATCAGCAAC TCAGGGGGAC CTGTACGAGT CTATAGCCTA 
CCTGGTCGAG AAAACTATTC CTCAGTAGAT GCCAATGGCA TCCAGTCTCA AATGCTTTCT 
AGATGGTCTG CTTCTTTCAC AGTAACTAAA GGCAAAAGTA GTACACAGGA GGCCACAGGA 
CAAGCAGTGT CCACAGCACA TCCACCAACA GGTAAACGAC TAAAGAAAAC ACCCGAGAAG 
AAAACTGGCA ATAAAGATTG TAAAGCAGAC ATTGCATTTC TGATTGATGG AAGCTTTAAT 
ATTGGGCAGC GCCGATTTAA TTTACAGAAG AATTTTGTTG GAAAAGTGGC TCTAATGTTG 
GGAATTGGAA CAGAAGGACC ACATGTGGGC CTTGTTCAAG CCAGTGAACA TCCCAAAATA 
GAATTTTACT TGAAAAACTT TACATCAGCC AAAGATGTTT TGTTTGCCAT AAAGGAAGTA 
GGTTTCAGAG GGGGTAATTC CAATACAGGA AAAGCCTTGA AGCATACTGC TCAGAAATTC 
TTCACGGTAG ATGCTGGAGT AAGAAAAGGG ATCCCCAAAG TGGTGGTGGT ATTTATTGAT 
GGTTGGCCTT CTGATGACAT CGAGGAAGCA GGCATTGTGG CCAGAGAGTT TGGTGTCAAT 
GTATTTATAG TTTCTGTGGC CAAGCCTATC CCTGAAGAAC TGGGGATGGT TCAGGATGTC 
ACATTTGTTG ACAAGGCTGT CTGTCGGAAT AATGGCTTCT TCTCTTACCA CATGCCCAAC 
TGGTTTGGCA CCACAAAATA CGTAAAGCCT CTGGTACAGA AGCTGTGCAC TCATGAACAA 
ATGATGTGCA GCAAGACCTG TTATAACTCA GTGAACATTG CCTTTCTAAT TGATGGCTCC 
AGCAGTGTTG GAGATAGCAA TTTCCGCCTC ATGCTTGAAT TTGTTTCCAA CATAGCCAAG 
ACTTTTGAAA TCTCGGACAT TGGTGCCAAG ATAGCTGCTG TACAGTTTAC TTATGATCAG 
CGCACGGAGT TCAGTTTCAC TGACTATAGC ACCAAAGAGA ATGTCCTAGC TGTCATCAGA 
AACATCCGCT ATATGAGTGG TGGAACAGCT ACTGGTGATG CCATTTCCTT CACTGTTAGA 
AATGTGTTTG GCCCTATAAG GGAGAGCCCC AACAAGAACT TCCTAGTAAT TGTCACAGAT 
GGGCAGTCCT ATGATGATGT CCAAGGCCCT GCAGCTGCTG CACATGATGC AGGAATCACT 
ATCTTCTCTG TTGGTGTGGC TTGGGCACCT CTGGATGACC TGAAAGATAT GGCTTCTAAA 
CCGAAGGAGT CTCATGCTTT CTTCACAAGA GAGTTCACAG GATTAGAACC AATTGTTTCT 
GATGTCATCA GAGGCATTTG TAGAGATTTC TTAGAATCCC AGCAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COCH ( XM_024449506.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024305274.1 Homo sapiens cochlin (COCH), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu103870
Clone ID Related Accession (Same CDS sequence)	XM_024449506.1
Accession Version	XM_024449506.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1710bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	cochlin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000014.9) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGCAG CCTGGATCCC GGCTCTCGGC CTCGGTGTGT GTCTGCTGCT GCTGCCGGGG 
CCCGCGGGCA GCGAGGGAGC CGCTCCCATT GCTATCACAT GTTTTACCAG AGGCTTGGAC 
ATCAGGAAAG AGAAAGCAGA TGTCCTCTGC CCAGGGGGCT GCCCTCTTGA GGAATTCTCT 
GTGTATGGGA ACATAGTATA TGCTTCTGTA TCGAGCATAT GTGGGGCTGC TGTCCACAGG 
GGAGTAATCA GCAACTCAGG GGGACCTGTA CGAGTCTATA GCCTACCTGG TCGAGAAAAC 
TATTCCTCAG TAGATGCCAA TGGCATCCAG TCTCAAATGC TTTCTAGATG GTCTGCTTCT 
TTCACAGTAA CTAAAGGCAA AAGTAGTACA CAGGAGGCCA CAGGACAAGC AGTGTCCACA 
GCACATCCAC CAACAGGTAA ACGACTAAAG AAAACACCCG AGAAGAAAAC TGGCAATAAA 
GATTGTAAAG CAGACATTGC ATTTCTGATT GATGGAAGCT TTAATATTGG GCAGCGCCGA 
TTTAATTTAC AGAAGAATTT TGTTGGAAAA GTGGCTCTAA TGTTGGGAAT TGGAACAGAA 
GGACCACATG TGGGCCTTGT TCAAGCCAGT GAACATCCCA AAATAGAATT TTACTTGAAA 
AACTTTACAT CAGCCAAAGA TGTTTTGTTT GCCATAAAGG AAGTAGGTTT CAGAGGGGGT 
AATTCCAATA CAGATGTGGC AGAAAGAATG AGTAGCACTA CCGTTGACTC TGAAGAGAGA 
CTTCTTAGAG GAAAAGCCTT GAAGCATACT GCTCAGAAAT TCTTCACGGT AGATGCTGGA 
GTAAGAAAAG GGATCCCCAA AGTGGTGGTG GTATTTATTG ATGGTTGGCC TTCTGATGAC 
ATCGAGGAAG CAGGCATTGT GGCCAGAGAG TTTGGTGTCA ATGTATTTAT AGTTTCTGTG 
GCCAAGCCTA TCCCTGAAGA ACTGGGGATG GTTCAGGATG TCACATTTGT TGACAAGGCT 
GTCTGTCGGA ATAATGGCTT CTTCTCTTAC CACATGCCCA ACTGGTTTGG CACCACAAAA 
TACGTAAAGC CTCTGGTACA GAAGCTGTGC ACTCATGAAC AAATGATGTG CAGCAAGACC 
TGTTATAACT CAGTGAACAT TGCCTTTCTA ATTGATGGCT CCAGCAGTGT TGGAGATAGC 
AATTTCCGCC TCATGCTTGA ATTTGTTTCC AACATAGCCA AGACTTTTGA AATCTCGGAC 
ATTGGTGCCA AGATAGCTGC TGTACAGTTT ACTTATGATC AGCGCACGGA GTTCAGTTTC 
ACTGACTATA GCACCAAAGA GAATGTCCTA GCTGTCATCA GAAACATCCG CTATATGAGT 
GGTGGAACAG CTACTGGTGA TGCCATTTCC TTCACTGTTA GAAATGTGTT TGGCCCTATA 
AGGGAGAGCC CCAACAAGAA CTTCCTAGTA ATTGTCACAG ATGGGCAGTC CTATGATGAT 
GTCCAAGGCC CTGCAGCTGC TGCACATGAT GCAGGAATCA CTATCTTCTC TGTTGGTGTG 
GCTTGGGCAC CTCTGGATGA CCTGAAAGAT ATGGCTTCTA AACCGAAGGA GTCTCATGCT 
TTCTTCACAA GAGAGTTCAC AGGATTAGAA CCAATTGTTT CTGATGTCAT CAGAGGCATT 
TGTAGAGATT TCTTAGAATC CCAGCAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024305274.1
CDS	57..1766
Translation	MSAAWIPALGLGVCLLLLPGPAGSEGAAPIAITCFTRGLDIRKEKADVLCPGGCPLEEFSVYGNIVYASVSSICGAAVHRGVISNSGGPVRVYSLPGRENYSSVDANGIQSQMLSRWSASFTVTKGKSSTQEATGQAVSTAHPPTGKRLKKTPEKKTGNKDCKADIAFLIDGSFNIGQRRFNLQKNFVGKVALMLGIGTEGPHVGLVQASEHPKIEFYLKNFTSAKDVLFAIKEVGFRGGNSNTDVAERMSSTTVDSEERLLRGKALKHTAQKFFTVDAGVRKGIPKVVVVFIDGWPSDDIEEAGIVAREFGVNVFIVSVAKPIPEELGMVQDVTFVDKAVCRNNGFFSYHMPNWFGTTKYVKPLVQKLCTHEQMMCSKTCYNSVNIAFLIDGSSSVGDSNFRLMLEFVSNIAKTFEISDIGAKIAAVQFTYDQRTEFSFTDYSTKENVLAVIRNIRYMSGGTATGDAISFTVRNVFGPIRESPNKNFLVIVTDGQSYDDVQGPAAAAHDAGITIFSVGVAWAPLDDLKDMASKPKESHAFFTREFTGLEPIVSDVIRGICRDFLESQQ

Target ORF information:

RefSeq Version	XM_024449506.1
Organism	Homo sapiens(human)
Definition	Homo sapiens cochlin (COCH), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024449506.1

ORF Insert Sequence:

ATGTCCGCAG CCTGGATCCC GGCTCTCGGC CTCGGTGTGT GTCTGCTGCT GCTGCCGGGG 
CCCGCGGGCA GCGAGGGAGC CGCTCCCATT GCTATCACAT GTTTTACCAG AGGCTTGGAC 
ATCAGGAAAG AGAAAGCAGA TGTCCTCTGC CCAGGGGGCT GCCCTCTTGA GGAATTCTCT 
GTGTATGGGA ACATAGTATA TGCTTCTGTA TCGAGCATAT GTGGGGCTGC TGTCCACAGG 
GGAGTAATCA GCAACTCAGG GGGACCTGTA CGAGTCTATA GCCTACCTGG TCGAGAAAAC 
TATTCCTCAG TAGATGCCAA TGGCATCCAG TCTCAAATGC TTTCTAGATG GTCTGCTTCT 
TTCACAGTAA CTAAAGGCAA AAGTAGTACA CAGGAGGCCA CAGGACAAGC AGTGTCCACA 
GCACATCCAC CAACAGGTAA ACGACTAAAG AAAACACCCG AGAAGAAAAC TGGCAATAAA 
GATTGTAAAG CAGACATTGC ATTTCTGATT GATGGAAGCT TTAATATTGG GCAGCGCCGA 
TTTAATTTAC AGAAGAATTT TGTTGGAAAA GTGGCTCTAA TGTTGGGAAT TGGAACAGAA 
GGACCACATG TGGGCCTTGT TCAAGCCAGT GAACATCCCA AAATAGAATT TTACTTGAAA 
AACTTTACAT CAGCCAAAGA TGTTTTGTTT GCCATAAAGG AAGTAGGTTT CAGAGGGGGT 
AATTCCAATA CAGATGTGGC AGAAAGAATG AGTAGCACTA CCGTTGACTC TGAAGAGAGA 
CTTCTTAGAG GAAAAGCCTT GAAGCATACT GCTCAGAAAT TCTTCACGGT AGATGCTGGA 
GTAAGAAAAG GGATCCCCAA AGTGGTGGTG GTATTTATTG ATGGTTGGCC TTCTGATGAC 
ATCGAGGAAG CAGGCATTGT GGCCAGAGAG TTTGGTGTCA ATGTATTTAT AGTTTCTGTG 
GCCAAGCCTA TCCCTGAAGA ACTGGGGATG GTTCAGGATG TCACATTTGT TGACAAGGCT 
GTCTGTCGGA ATAATGGCTT CTTCTCTTAC CACATGCCCA ACTGGTTTGG CACCACAAAA 
TACGTAAAGC CTCTGGTACA GAAGCTGTGC ACTCATGAAC AAATGATGTG CAGCAAGACC 
TGTTATAACT CAGTGAACAT TGCCTTTCTA ATTGATGGCT CCAGCAGTGT TGGAGATAGC 
AATTTCCGCC TCATGCTTGA ATTTGTTTCC AACATAGCCA AGACTTTTGA AATCTCGGAC 
ATTGGTGCCA AGATAGCTGC TGTACAGTTT ACTTATGATC AGCGCACGGA GTTCAGTTTC 
ACTGACTATA GCACCAAAGA GAATGTCCTA GCTGTCATCA GAAACATCCG CTATATGAGT 
GGTGGAACAG CTACTGGTGA TGCCATTTCC TTCACTGTTA GAAATGTGTT TGGCCCTATA 
AGGGAGAGCC CCAACAAGAA CTTCCTAGTA ATTGTCACAG ATGGGCAGTC CTATGATGAT 
GTCCAAGGCC CTGCAGCTGC TGCACATGAT GCAGGAATCA CTATCTTCTC TGTTGGTGTG 
GCTTGGGCAC CTCTGGATGA CCTGAAAGAT ATGGCTTCTA AACCGAAGGA GTCTCATGCT 
TTCTTCACAA GAGAGTTCAC AGGATTAGAA CCAATTGTTT CTGATGTCAT CAGAGGCATT 
TGTAGAGATT TCTTAGAATC CCAGCAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COCH ( XM_017021071.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016876560.1 Homo sapiens cochlin (COCH), transcript variant X1, mRNA.

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CloneID	OHu84817
Clone ID Related Accession (Same CDS sequence)	XM_017021071.1 , NM_001347720.1
Accession Version	XM_017021071.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1848bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	cochlin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000014.9) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGCAG CCTGGATCCC GGCTCTCGGC CTCGGTGTGT GTCTGCTGCT GCTGCCGGGG 
CCCGCGGGCA GCGAGGGAGC CGGTGAGTGG GGGAGCTGGG GTGCGTCCAG GCGGTCGCAG 
GGGCTGAGCA CCAGCGGGTA CAAGCGGGAC TCAGATCCAG CCCCTTGGGC TTCAGCCCTA 
CCGCCTGAGG AGGAAGGCGC GAAGGTTGAG CCGCCGCGTG GCGCGCCCGC GTTAACCCCT 
GCAGCCGATC TGCTCCTGCT CACCTGTTTC TCCCATGCTC CCATTGCTAT CACATGTTTT 
ACCAGAGGCT TGGACATCAG GAAAGAGAAA GCAGATGTCC TCTGCCCAGG GGGCTGCCCT 
CTTGAGGAAT TCTCTGTGTA TGGGAACATA GTATATGCTT CTGTATCGAG CATATGTGGG 
GCTGCTGTCC ACAGGGGAGT AATCAGCAAC TCAGGGGGAC CTGTACGAGT CTATAGCCTA 
CCTGGTCGAG AAAACTATTC CTCAGTAGAT GCCAATGGCA TCCAGTCTCA AATGCTTTCT 
AGATGGTCTG CTTCTTTCAC AGTAACTAAA GGCAAAAGTA GTACACAGGA GGCCACAGGA 
CAAGCAGTGT CCACAGCACA TCCACCAACA GGTAAACGAC TAAAGAAAAC ACCCGAGAAG 
AAAACTGGCA ATAAAGATTG TAAAGCAGAC ATTGCATTTC TGATTGATGG AAGCTTTAAT 
ATTGGGCAGC GCCGATTTAA TTTACAGAAG AATTTTGTTG GAAAAGTGGC TCTAATGTTG 
GGAATTGGAA CAGAAGGACC ACATGTGGGC CTTGTTCAAG CCAGTGAACA TCCCAAAATA 
GAATTTTACT TGAAAAACTT TACATCAGCC AAAGATGTTT TGTTTGCCAT AAAGGAAGTA 
GGTTTCAGAG GGGGTAATTC CAATACAGGA AAAGCCTTGA AGCATACTGC TCAGAAATTC 
TTCACGGTAG ATGCTGGAGT AAGAAAAGGG ATCCCCAAAG TGGTGGTGGT ATTTATTGAT 
GGTTGGCCTT CTGATGACAT CGAGGAAGCA GGCATTGTGG CCAGAGAGTT TGGTGTCAAT 
GTATTTATAG TTTCTGTGGC CAAGCCTATC CCTGAAGAAC TGGGGATGGT TCAGGATGTC 
ACATTTGTTG ACAAGGCTGT CTGTCGGAAT AATGGCTTCT TCTCTTACCA CATGCCCAAC 
TGGTTTGGCA CCACAAAATA CGTAAAGCCT CTGGTACAGA AGCTGTGCAC TCATGAACAA 
ATGATGTGCA GCAAGACCTG TTATAACTCA GTGAACATTG CCTTTCTAAT TGATGGCTCC 
AGCAGTGTTG GAGATAGCAA TTTCCGCCTC ATGCTTGAAT TTGTTTCCAA CATAGCCAAG 
ACTTTTGAAA TCTCGGACAT TGGTGCCAAG ATAGCTGCTG TACAGTTTAC TTATGATCAG 
CGCACGGAGT TCAGTTTCAC TGACTATAGC ACCAAAGAGA ATGTCCTAGC TGTCATCAGA 
AACATCCGCT ATATGAGTGG TGGAACAGCT ACTGGTGATG CCATTTCCTT CACTGTTAGA 
AATGTGTTTG GCCCTATAAG GGAGAGCCCC AACAAGAACT TCCTAGTAAT TGTCACAGAT 
GGGCAGTCCT ATGATGATGT CCAAGGCCCT GCAGCTGCTG CACATGATGC AGGAATCACT 
ATCTTCTCTG TTGGTGTGGC TTGGGCACCT CTGGATGACC TGAAAGATAT GGCTTCTAAA 
CCGAAGGAGT CTCATGCTTT CTTCACAAGA GAGTTCACAG GATTAGAACC AATTGTTTCT 
GATGTCATCA GAGGCATTTG TAGAGATTTC TTAGAATCCC AGCAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016876560.1
CDS	120..1967
Translation	MSAAWIPALGLGVCLLLLPGPAGSEGAGEWGSWGASRRSQGLSTSGYKRDSDPAPWASALPPEEEGAKVEPPRGAPALTPAADLLLLTCFSHAPIAITCFTRGLDIRKEKADVLCPGGCPLEEFSVYGNIVYASVSSICGAAVHRGVISNSGGPVRVYSLPGRENYSSVDANGIQSQMLSRWSASFTVTKGKSSTQEATGQAVSTAHPPTGKRLKKTPEKKTGNKDCKADIAFLIDGSFNIGQRRFNLQKNFVGKVALMLGIGTEGPHVGLVQASEHPKIEFYLKNFTSAKDVLFAIKEVGFRGGNSNTGKALKHTAQKFFTVDAGVRKGIPKVVVVFIDGWPSDDIEEAGIVAREFGVNVFIVSVAKPIPEELGMVQDVTFVDKAVCRNNGFFSYHMPNWFGTTKYVKPLVQKLCTHEQMMCSKTCYNSVNIAFLIDGSSSVGDSNFRLMLEFVSNIAKTFEISDIGAKIAAVQFTYDQRTEFSFTDYSTKENVLAVIRNIRYMSGGTATGDAISFTVRNVFGPIRESPNKNFLVIVTDGQSYDDVQGPAAAAHDAGITIFSVGVAWAPLDDLKDMASKPKESHAFFTREFTGLEPIVSDVIRGICRDFLESQQ

Target ORF information:

RefSeq Version	XM_017021071.1
Organism	Homo sapiens(human)
Definition	Homo sapiens cochlin (COCH), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017021071.1

ORF Insert Sequence:

ATGTCCGCAG CCTGGATCCC GGCTCTCGGC CTCGGTGTGT GTCTGCTGCT GCTGCCGGGG 
CCCGCGGGCA GCGAGGGAGC CGGTGAGTGG GGGAGCTGGG GTGCGTCCAG GCGGTCGCAG 
GGGCTGAGCA CCAGCGGGTA CAAGCGGGAC TCAGATCCAG CCCCTTGGGC TTCAGCCCTA 
CCGCCTGAGG AGGAAGGCGC GAAGGTTGAG CCGCCGCGTG GCGCGCCCGC GTTAACCCCT 
GCAGCCGATC TGCTCCTGCT CACCTGTTTC TCCCATGCTC CCATTGCTAT CACATGTTTT 
ACCAGAGGCT TGGACATCAG GAAAGAGAAA GCAGATGTCC TCTGCCCAGG GGGCTGCCCT 
CTTGAGGAAT TCTCTGTGTA TGGGAACATA GTATATGCTT CTGTATCGAG CATATGTGGG 
GCTGCTGTCC ACAGGGGAGT AATCAGCAAC TCAGGGGGAC CTGTACGAGT CTATAGCCTA 
CCTGGTCGAG AAAACTATTC CTCAGTAGAT GCCAATGGCA TCCAGTCTCA AATGCTTTCT 
AGATGGTCTG CTTCTTTCAC AGTAACTAAA GGCAAAAGTA GTACACAGGA GGCCACAGGA 
CAAGCAGTGT CCACAGCACA TCCACCAACA GGTAAACGAC TAAAGAAAAC ACCCGAGAAG 
AAAACTGGCA ATAAAGATTG TAAAGCAGAC ATTGCATTTC TGATTGATGG AAGCTTTAAT 
ATTGGGCAGC GCCGATTTAA TTTACAGAAG AATTTTGTTG GAAAAGTGGC TCTAATGTTG 
GGAATTGGAA CAGAAGGACC ACATGTGGGC CTTGTTCAAG CCAGTGAACA TCCCAAAATA 
GAATTTTACT TGAAAAACTT TACATCAGCC AAAGATGTTT TGTTTGCCAT AAAGGAAGTA 
GGTTTCAGAG GGGGTAATTC CAATACAGGA AAAGCCTTGA AGCATACTGC TCAGAAATTC 
TTCACGGTAG ATGCTGGAGT AAGAAAAGGG ATCCCCAAAG TGGTGGTGGT ATTTATTGAT 
GGTTGGCCTT CTGATGACAT CGAGGAAGCA GGCATTGTGG CCAGAGAGTT TGGTGTCAAT 
GTATTTATAG TTTCTGTGGC CAAGCCTATC CCTGAAGAAC TGGGGATGGT TCAGGATGTC 
ACATTTGTTG ACAAGGCTGT CTGTCGGAAT AATGGCTTCT TCTCTTACCA CATGCCCAAC 
TGGTTTGGCA CCACAAAATA CGTAAAGCCT CTGGTACAGA AGCTGTGCAC TCATGAACAA 
ATGATGTGCA GCAAGACCTG TTATAACTCA GTGAACATTG CCTTTCTAAT TGATGGCTCC 
AGCAGTGTTG GAGATAGCAA TTTCCGCCTC ATGCTTGAAT TTGTTTCCAA CATAGCCAAG 
ACTTTTGAAA TCTCGGACAT TGGTGCCAAG ATAGCTGCTG TACAGTTTAC TTATGATCAG 
CGCACGGAGT TCAGTTTCAC TGACTATAGC ACCAAAGAGA ATGTCCTAGC TGTCATCAGA 
AACATCCGCT ATATGAGTGG TGGAACAGCT ACTGGTGATG CCATTTCCTT CACTGTTAGA 
AATGTGTTTG GCCCTATAAG GGAGAGCCCC AACAAGAACT TCCTAGTAAT TGTCACAGAT 
GGGCAGTCCT ATGATGATGT CCAAGGCCCT GCAGCTGCTG CACATGATGC AGGAATCACT 
ATCTTCTCTG TTGGTGTGGC TTGGGCACCT CTGGATGACC TGAAAGATAT GGCTTCTAAA 
CCGAAGGAGT CTCATGCTTT CTTCACAAGA GAGTTCACAG GATTAGAACC AATTGTTTCT 
GATGTCATCA GAGGCATTTG TAGAGATTTC TTAGAATCCC AGCAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Impaired lymphoid extracellular matrix impedes antibacterial immunity in epidermolysis bullosa.
Proceedings of the National Academy of Sciences of the United States of America(2018 Jan)
Nystr?m A,Bornert O,K?hl T,Gretzmeier C,Thriene K,Dengjel J,Pfister-Wartha A,Kiritsi D,Bruckner-Tuderman L

Different Phenotypes of the Two Chinese Probands with the Same c.889G>A (p.C162Y) Mutation in COCH Gene Verify Different Mechanisms Underlying Autosomal Dominant Nonsyndromic Deafness 9.
PloS one12(1)e0170011(2017)
Wang Q,Fei P,Gu H,Zhang Y,Ke X,Liu Y

Massively Parallel Sequencing of a Chinese Family with DFNA9 Identified a Novel Missense Mutation in the LCCL Domain of COCH.
Neural plasticity20165310192(2016)
Gu X,Su W,Tang M,Guo L,Zhao L,Li H

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COCH cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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