GSDME cDNA ORF clone, Homo sapiens(human)

The following GSDME gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GSDME cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu25942 NM_001127453.2
Latest version!
Homo sapiens gasdermin E (GSDME), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$159.50-$223.30
$319.00
OHu25942 NM_004403.3
Latest version!
Homo sapiens gasdermin E (GSDME), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$159.50-$223.30
$319.00
OHu26331 NM_001127454.2
Latest version!
Homo sapiens gasdermin E (GSDME), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$99.50-$139.30
$199.00
OHu25942 XM_024446670.1
Latest version!
Homo sapiens gasdermin E (GSDME), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$159.50-$223.30
$319.00
OHu26331 XM_017011802.1
Latest version!
Homo sapiens gasdermin E (GSDME), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$99.50-$139.30
$199.00
View Old Accession Versions >>
OHu25942 NM_004403.2 Homo sapiens gasdermin E (GSDME), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$159.50-$223.30
$319.00
OHu26331 NM_001127454.1 Homo sapiens gasdermin E (GSDME), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$99.50-$139.30
$199.00
OHu25942 NM_001127453.1 Homo sapiens gasdermin E (GSDME), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$159.50-$223.30
$319.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu25942
    Clone ID Related Accession (Same CDS sequence) NM_004403.3 , XM_024446670.1 , NM_004403.2 , NM_001127453.1 , NM_001127453.2
    Accession Version NM_004403.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1491bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-11-25
    Organism Homo sapiens(human)
    Product gasdermin-E isoform a
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC003093.1, AF073308.1, BC125065.1 and AF131765.1. On Nov 23, 2018 this sequence version replaced NM_004403.2. Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (a). Variants 1 and 2 both encode isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.74450.1, SRR1803615.207378.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGTTTGCCA AAGCAACCAG GAATTTTCTT AGAGAAGTTG ATGCTGATGG TGACCTGATT 
    GCAGTATCAA ATCTGAATGA CTCTGATAAG TTACAGCTTC TAAGTCTGGT GACAAAAAAG
    AAGAGATTCT GGTGCTGGCA GAGACCCAAG TACCAGTTTT TATCCCTCAC CCTTGGCGAT
    GTACTCATAG AAGACCAATT TCCGAGTCCA GTGGTCGTGG AGTCGGACTT TGTGAAATAC
    GAGGGCAAGT TTGCAAACCA CGTGAGTGGA ACCCTGGAGA CTGCACTGGG GAAGGTCAAG
    CTGAACCTGG GGGGCAGCAG CCGCGTAGAG AGCCAGTCTT CATTTGGAAC CCTGAGGAAG
    CAGGAGGTGG ATTTGCAGCA GCTCATCAGA GACTCTGCCG AGAGAACAAT AAATCTGAGA
    AACCCTGTGC TCCAGCAGGT GCTGGAAGGA AGGAATGAGG TCCTGTGCGT TTTGACACAG
    AAGATCACGA CGATGCAGAA GTGTGTGATC TCTGAGCACA TGCAGGTCGA GGAGAAGTGT
    GGTGGCATCG TGGGCATCCA GACCAAGACG GTGCAGGTGT CAGCGACGGA GGATGGGAAT
    GTCACCAAGG ACTCCAACGT GGTGCTGGAG ATCCCAGCTG CCACCACCAT TGCCTACGGT
    GTCATTGAGT TATACGTGAA ACTGGACGGC CAGTTCGAGT TCTGCCTTCT CCGAGGGAAG
    CAAGGTGGCT TCGAGAACAA GAAGAGAATT GACTCTGTCT ACCTGGACCC CCTGGTCTTT
    CGAGAGTTTG CATTCATAGA CATGCCAGAT GCTGCGCATG GGATATCTTC CCAGGATGGA
    CCATTAAGTG TTTTAAAGCA AGCGACCCTG CTCCTGGAGA GGAATTTCCA TCCATTTGCG
    GAGCTGCCTG AGCCACAACA GACAGCTTTG AGTGACATCT TCCAGGCGGT CCTATTTGAT
    GATGAACTAC TCATGGTCCT GGAACCAGTG TGCGATGACC TGGTCAGCGG CCTCTCGCCC
    ACAGTGGCGG TGCTGGGGGA GCTGAAGCCC CGGCAGCAGC AGGACCTTGT GGCCTTCCTG
    CAGCTGGTGG GGTGCAGCTT ACAGGGTGGG TGTCCGGGCC CCGAGGATGC AGGCAGCAAG
    CAGCTGTTTA TGACAGCCTA CTTCTTGGTC AGTGCCCTCG CAGAAATGCC AGATAGCGCA
    GCAGCTCTGC TGGGCACTTG CTGCAAACTC CAGATCATTC CCACACTGTG CCACTTGCTT
    CGTGCTCTGT CTGATGATGG AGTATCTGAT CTTGAAGACC CAACCTTGAC TCCCCTGAAA
    GATACAGAAA GGTTTGGGAT TGTGCAGCGC TTGTTTGCCT CAGCTGACAT TAGTCTGGAG
    AGACTGAAGT CATCTGTGAA AGCTGTCATT CTGAAGGACT CTAAAGTCTT CCCACTGCTT
    CTTTGTATAA CCCTGAATGG ACTCTGTGCT TTAGGCAGAG AACATTCATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_004394.1
    CDS253..1743
    Translation

    Target ORF information:

    RefSeq Version NM_004403.3
    Organism Homo sapiens(human)
    Definition Homo sapiens gasdermin E (GSDME), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_004403.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGTTTGCCA AAGCAACCAG GAATTTTCTT AGAGAAGTTG ATGCTGATGG TGACCTGATT 
    GCAGTATCAA ATCTGAATGA CTCTGATAAG TTACAGCTTC TAAGTCTGGT GACAAAAAAG
    AAGAGATTCT GGTGCTGGCA GAGACCCAAG TACCAGTTTT TATCCCTCAC CCTTGGCGAT
    GTACTCATAG AAGACCAATT TCCGAGTCCA GTGGTCGTGG AGTCGGACTT TGTGAAATAC
    GAGGGCAAGT TTGCAAACCA CGTGAGTGGA ACCCTGGAGA CTGCACTGGG GAAGGTCAAG
    CTGAACCTGG GGGGCAGCAG CCGCGTAGAG AGCCAGTCTT CATTTGGAAC CCTGAGGAAG
    CAGGAGGTGG ATTTGCAGCA GCTCATCAGA GACTCTGCCG AGAGAACAAT AAATCTGAGA
    AACCCTGTGC TCCAGCAGGT GCTGGAAGGA AGGAATGAGG TCCTGTGCGT TTTGACACAG
    AAGATCACGA CGATGCAGAA GTGTGTGATC TCTGAGCACA TGCAGGTCGA GGAGAAGTGT
    GGTGGCATCG TGGGCATCCA GACCAAGACG GTGCAGGTGT CAGCGACGGA GGATGGGAAT
    GTCACCAAGG ACTCCAACGT GGTGCTGGAG ATCCCAGCTG CCACCACCAT TGCCTACGGT
    GTCATTGAGT TATACGTGAA ACTGGACGGC CAGTTCGAGT TCTGCCTTCT CCGAGGGAAG
    CAAGGTGGCT TCGAGAACAA GAAGAGAATT GACTCTGTCT ACCTGGACCC CCTGGTCTTT
    CGAGAGTTTG CATTCATAGA CATGCCAGAT GCTGCGCATG GGATATCTTC CCAGGATGGA
    CCATTAAGTG TTTTAAAGCA AGCGACCCTG CTCCTGGAGA GGAATTTCCA TCCATTTGCG
    GAGCTGCCTG AGCCACAACA GACAGCTTTG AGTGACATCT TCCAGGCGGT CCTATTTGAT
    GATGAACTAC TCATGGTCCT GGAACCAGTG TGCGATGACC TGGTCAGCGG CCTCTCGCCC
    ACAGTGGCGG TGCTGGGGGA GCTGAAGCCC CGGCAGCAGC AGGACCTTGT GGCCTTCCTG
    CAGCTGGTGG GGTGCAGCTT ACAGGGTGGG TGTCCGGGCC CCGAGGATGC AGGCAGCAAG
    CAGCTGTTTA TGACAGCCTA CTTCTTGGTC AGTGCCCTCG CAGAAATGCC AGATAGCGCA
    GCAGCTCTGC TGGGCACTTG CTGCAAACTC CAGATCATTC CCACACTGTG CCACTTGCTT
    CGTGCTCTGT CTGATGATGG AGTATCTGAT CTTGAAGACC CAACCTTGAC TCCCCTGAAA
    GATACAGAAA GGTTTGGGAT TGTGCAGCGC TTGTTTGCCT CAGCTGACAT TAGTCTGGAG
    AGACTGAAGT CATCTGTGAA AGCTGTCATT CTGAAGGACT CTAAAGTCTT CCCACTGCTT
    CTTTGTATAA CCCTGAATGG ACTCTGTGCT TTAGGCAGAG AACATTCATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu25942
    Clone ID Related Accession (Same CDS sequence) NM_004403.3 , XM_024446670.1 , NM_004403.2 , NM_001127453.1 , NM_001127453.2
    Accession Version NM_004403.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1491bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-12-31
    Organism Homo sapiens(human)
    Product gasdermin-E isoform a
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC003093.1, AF073308.1, BC125065.1 and AF131765.1. This sequence is a reference standard in the RefSeqGene project. On Oct 26, 2006 this sequence version replaced NM_004403.1. Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (a). Variants 1 and 2 both encode isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.74450.1, SRR1803614.243516.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGTTTGCCA AAGCAACCAG GAATTTTCTT AGAGAAGTTG ATGCTGATGG TGACCTGATT 
    GCAGTATCAA ATCTGAATGA CTCTGATAAG TTACAGCTTC TAAGTCTGGT GACAAAAAAG
    AAGAGATTCT GGTGCTGGCA GAGACCCAAG TACCAGTTTT TATCCCTCAC CCTTGGCGAT
    GTACTCATAG AAGACCAATT TCCGAGTCCA GTGGTCGTGG AGTCGGACTT TGTGAAATAC
    GAGGGCAAGT TTGCAAACCA CGTGAGTGGA ACCCTGGAGA CTGCACTGGG GAAGGTCAAG
    CTGAACCTGG GGGGCAGCAG CCGCGTAGAG AGCCAGTCTT CATTTGGAAC CCTGAGGAAG
    CAGGAGGTGG ATTTGCAGCA GCTCATCAGA GACTCTGCCG AGAGAACAAT AAATCTGAGA
    AACCCTGTGC TCCAGCAGGT GCTGGAAGGA AGGAATGAGG TCCTGTGCGT TTTGACACAG
    AAGATCACGA CGATGCAGAA GTGTGTGATC TCTGAGCACA TGCAGGTCGA GGAGAAGTGT
    GGTGGCATCG TGGGCATCCA GACCAAGACG GTGCAGGTGT CAGCGACGGA GGATGGGAAT
    GTCACCAAGG ACTCCAACGT GGTGCTGGAG ATCCCAGCTG CCACCACCAT TGCCTACGGT
    GTCATTGAGT TATACGTGAA ACTGGACGGC CAGTTCGAGT TCTGCCTTCT CCGAGGGAAG
    CAAGGTGGCT TCGAGAACAA GAAGAGAATT GACTCTGTCT ACCTGGACCC CCTGGTCTTT
    CGAGAGTTTG CATTCATAGA CATGCCAGAT GCTGCGCATG GGATATCTTC CCAGGATGGA
    CCATTAAGTG TTTTAAAGCA AGCGACCCTG CTCCTGGAGA GGAATTTCCA TCCATTTGCG
    GAGCTGCCTG AGCCACAACA GACAGCTTTG AGTGACATCT TCCAGGCGGT CCTATTTGAT
    GATGAACTAC TCATGGTCCT GGAACCAGTG TGCGATGACC TGGTCAGCGG CCTCTCGCCC
    ACAGTGGCGG TGCTGGGGGA GCTGAAGCCC CGGCAGCAGC AGGACCTTGT GGCCTTCCTG
    CAGCTGGTGG GGTGCAGCTT ACAGGGTGGG TGTCCGGGCC CCGAGGATGC AGGCAGCAAG
    CAGCTGTTTA TGACAGCCTA CTTCTTGGTC AGTGCCCTCG CAGAAATGCC AGATAGCGCA
    GCAGCTCTGC TGGGCACTTG CTGCAAACTC CAGATCATTC CCACACTGTG CCACTTGCTT
    CGTGCTCTGT CTGATGATGG AGTATCTGAT CTTGAAGACC CAACCTTGAC TCCCCTGAAA
    GATACAGAAA GGTTTGGGAT TGTGCAGCGC TTGTTTGCCT CAGCTGACAT TAGTCTGGAG
    AGACTGAAGT CATCTGTGAA AGCTGTCATT CTGAAGGACT CTAAAGTCTT CCCACTGCTT
    CTTTGTATAA CCCTGAATGG ACTCTGTGCT TTAGGCAGAG AACATTCATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_004394.1
    CDS334..1824
    Translation

    Target ORF information:

    RefSeq Version NM_004403.2
    Organism Homo sapiens(human)
    Definition Homo sapiens gasdermin E (GSDME), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_004403.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGTTTGCCA AAGCAACCAG GAATTTTCTT AGAGAAGTTG ATGCTGATGG TGACCTGATT 
    GCAGTATCAA ATCTGAATGA CTCTGATAAG TTACAGCTTC TAAGTCTGGT GACAAAAAAG
    AAGAGATTCT GGTGCTGGCA GAGACCCAAG TACCAGTTTT TATCCCTCAC CCTTGGCGAT
    GTACTCATAG AAGACCAATT TCCGAGTCCA GTGGTCGTGG AGTCGGACTT TGTGAAATAC
    GAGGGCAAGT TTGCAAACCA CGTGAGTGGA ACCCTGGAGA CTGCACTGGG GAAGGTCAAG
    CTGAACCTGG GGGGCAGCAG CCGCGTAGAG AGCCAGTCTT CATTTGGAAC CCTGAGGAAG
    CAGGAGGTGG ATTTGCAGCA GCTCATCAGA GACTCTGCCG AGAGAACAAT AAATCTGAGA
    AACCCTGTGC TCCAGCAGGT GCTGGAAGGA AGGAATGAGG TCCTGTGCGT TTTGACACAG
    AAGATCACGA CGATGCAGAA GTGTGTGATC TCTGAGCACA TGCAGGTCGA GGAGAAGTGT
    GGTGGCATCG TGGGCATCCA GACCAAGACG GTGCAGGTGT CAGCGACGGA GGATGGGAAT
    GTCACCAAGG ACTCCAACGT GGTGCTGGAG ATCCCAGCTG CCACCACCAT TGCCTACGGT
    GTCATTGAGT TATACGTGAA ACTGGACGGC CAGTTCGAGT TCTGCCTTCT CCGAGGGAAG
    CAAGGTGGCT TCGAGAACAA GAAGAGAATT GACTCTGTCT ACCTGGACCC CCTGGTCTTT
    CGAGAGTTTG CATTCATAGA CATGCCAGAT GCTGCGCATG GGATATCTTC CCAGGATGGA
    CCATTAAGTG TTTTAAAGCA AGCGACCCTG CTCCTGGAGA GGAATTTCCA TCCATTTGCG
    GAGCTGCCTG AGCCACAACA GACAGCTTTG AGTGACATCT TCCAGGCGGT CCTATTTGAT
    GATGAACTAC TCATGGTCCT GGAACCAGTG TGCGATGACC TGGTCAGCGG CCTCTCGCCC
    ACAGTGGCGG TGCTGGGGGA GCTGAAGCCC CGGCAGCAGC AGGACCTTGT GGCCTTCCTG
    CAGCTGGTGG GGTGCAGCTT ACAGGGTGGG TGTCCGGGCC CCGAGGATGC AGGCAGCAAG
    CAGCTGTTTA TGACAGCCTA CTTCTTGGTC AGTGCCCTCG CAGAAATGCC AGATAGCGCA
    GCAGCTCTGC TGGGCACTTG CTGCAAACTC CAGATCATTC CCACACTGTG CCACTTGCTT
    CGTGCTCTGT CTGATGATGG AGTATCTGAT CTTGAAGACC CAACCTTGAC TCCCCTGAAA
    GATACAGAAA GGTTTGGGAT TGTGCAGCGC TTGTTTGCCT CAGCTGACAT TAGTCTGGAG
    AGACTGAAGT CATCTGTGAA AGCTGTCATT CTGAAGGACT CTAAAGTCTT CCCACTGCTT
    CTTTGTATAA CCCTGAATGG ACTCTGTGCT TTAGGCAGAG AACATTCATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu25942
    Clone ID Related Accession (Same CDS sequence) NM_004403.3 , XM_024446670.1 , NM_004403.2 , NM_001127453.1 , NM_001127453.2
    Accession Version NM_001127453.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1491bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-12-08
    Organism Homo sapiens(human)
    Product gasdermin-E isoform a
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA820184.1, BC125065.1 and AF131765.1. Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 and 2 both encode isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163655.287696.1, SRR1163657.205142.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGTTTGCCA AAGCAACCAG GAATTTTCTT AGAGAAGTTG ATGCTGATGG TGACCTGATT 
    GCAGTATCAA ATCTGAATGA CTCTGATAAG TTACAGCTTC TAAGTCTGGT GACAAAAAAG
    AAGAGATTCT GGTGCTGGCA GAGACCCAAG TACCAGTTTT TATCCCTCAC CCTTGGCGAT
    GTACTCATAG AAGACCAATT TCCGAGTCCA GTGGTCGTGG AGTCGGACTT TGTGAAATAC
    GAGGGCAAGT TTGCAAACCA CGTGAGTGGA ACCCTGGAGA CTGCACTGGG GAAGGTCAAG
    CTGAACCTGG GGGGCAGCAG CCGCGTAGAG AGCCAGTCTT CATTTGGAAC CCTGAGGAAG
    CAGGAGGTGG ATTTGCAGCA GCTCATCAGA GACTCTGCCG AGAGAACAAT AAATCTGAGA
    AACCCTGTGC TCCAGCAGGT GCTGGAAGGA AGGAATGAGG TCCTGTGCGT TTTGACACAG
    AAGATCACGA CGATGCAGAA GTGTGTGATC TCTGAGCACA TGCAGGTCGA GGAGAAGTGT
    GGTGGCATCG TGGGCATCCA GACCAAGACG GTGCAGGTGT CAGCGACGGA GGATGGGAAT
    GTCACCAAGG ACTCCAACGT GGTGCTGGAG ATCCCAGCTG CCACCACCAT TGCCTACGGT
    GTCATTGAGT TATACGTGAA ACTGGACGGC CAGTTCGAGT TCTGCCTTCT CCGAGGGAAG
    CAAGGTGGCT TCGAGAACAA GAAGAGAATT GACTCTGTCT ACCTGGACCC CCTGGTCTTT
    CGAGAGTTTG CATTCATAGA CATGCCAGAT GCTGCGCATG GGATATCTTC CCAGGATGGA
    CCATTAAGTG TTTTAAAGCA AGCGACCCTG CTCCTGGAGA GGAATTTCCA TCCATTTGCG
    GAGCTGCCTG AGCCACAACA GACAGCTTTG AGTGACATCT TCCAGGCGGT CCTATTTGAT
    GATGAACTAC TCATGGTCCT GGAACCAGTG TGCGATGACC TGGTCAGCGG CCTCTCGCCC
    ACAGTGGCGG TGCTGGGGGA GCTGAAGCCC CGGCAGCAGC AGGACCTTGT GGCCTTCCTG
    CAGCTGGTGG GGTGCAGCTT ACAGGGTGGG TGTCCGGGCC CCGAGGATGC AGGCAGCAAG
    CAGCTGTTTA TGACAGCCTA CTTCTTGGTC AGTGCCCTCG CAGAAATGCC AGATAGCGCA
    GCAGCTCTGC TGGGCACTTG CTGCAAACTC CAGATCATTC CCACACTGTG CCACTTGCTT
    CGTGCTCTGT CTGATGATGG AGTATCTGAT CTTGAAGACC CAACCTTGAC TCCCCTGAAA
    GATACAGAAA GGTTTGGGAT TGTGCAGCGC TTGTTTGCCT CAGCTGACAT TAGTCTGGAG
    AGACTGAAGT CATCTGTGAA AGCTGTCATT CTGAAGGACT CTAAAGTCTT CCCACTGCTT
    CTTTGTATAA CCCTGAATGG ACTCTGTGCT TTAGGCAGAG AACATTCATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001120925.1
    CDS89..1579
    Translation

    Target ORF information:

    RefSeq Version NM_001127453.1
    Organism Homo sapiens(human)
    Definition Homo sapiens gasdermin E (GSDME), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001127453.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGTTTGCCA AAGCAACCAG GAATTTTCTT AGAGAAGTTG ATGCTGATGG TGACCTGATT 
    GCAGTATCAA ATCTGAATGA CTCTGATAAG TTACAGCTTC TAAGTCTGGT GACAAAAAAG
    AAGAGATTCT GGTGCTGGCA GAGACCCAAG TACCAGTTTT TATCCCTCAC CCTTGGCGAT
    GTACTCATAG AAGACCAATT TCCGAGTCCA GTGGTCGTGG AGTCGGACTT TGTGAAATAC
    GAGGGCAAGT TTGCAAACCA CGTGAGTGGA ACCCTGGAGA CTGCACTGGG GAAGGTCAAG
    CTGAACCTGG GGGGCAGCAG CCGCGTAGAG AGCCAGTCTT CATTTGGAAC CCTGAGGAAG
    CAGGAGGTGG ATTTGCAGCA GCTCATCAGA GACTCTGCCG AGAGAACAAT AAATCTGAGA
    AACCCTGTGC TCCAGCAGGT GCTGGAAGGA AGGAATGAGG TCCTGTGCGT TTTGACACAG
    AAGATCACGA CGATGCAGAA GTGTGTGATC TCTGAGCACA TGCAGGTCGA GGAGAAGTGT
    GGTGGCATCG TGGGCATCCA GACCAAGACG GTGCAGGTGT CAGCGACGGA GGATGGGAAT
    GTCACCAAGG ACTCCAACGT GGTGCTGGAG ATCCCAGCTG CCACCACCAT TGCCTACGGT
    GTCATTGAGT TATACGTGAA ACTGGACGGC CAGTTCGAGT TCTGCCTTCT CCGAGGGAAG
    CAAGGTGGCT TCGAGAACAA GAAGAGAATT GACTCTGTCT ACCTGGACCC CCTGGTCTTT
    CGAGAGTTTG CATTCATAGA CATGCCAGAT GCTGCGCATG GGATATCTTC CCAGGATGGA
    CCATTAAGTG TTTTAAAGCA AGCGACCCTG CTCCTGGAGA GGAATTTCCA TCCATTTGCG
    GAGCTGCCTG AGCCACAACA GACAGCTTTG AGTGACATCT TCCAGGCGGT CCTATTTGAT
    GATGAACTAC TCATGGTCCT GGAACCAGTG TGCGATGACC TGGTCAGCGG CCTCTCGCCC
    ACAGTGGCGG TGCTGGGGGA GCTGAAGCCC CGGCAGCAGC AGGACCTTGT GGCCTTCCTG
    CAGCTGGTGG GGTGCAGCTT ACAGGGTGGG TGTCCGGGCC CCGAGGATGC AGGCAGCAAG
    CAGCTGTTTA TGACAGCCTA CTTCTTGGTC AGTGCCCTCG CAGAAATGCC AGATAGCGCA
    GCAGCTCTGC TGGGCACTTG CTGCAAACTC CAGATCATTC CCACACTGTG CCACTTGCTT
    CGTGCTCTGT CTGATGATGG AGTATCTGAT CTTGAAGACC CAACCTTGAC TCCCCTGAAA
    GATACAGAAA GGTTTGGGAT TGTGCAGCGC TTGTTTGCCT CAGCTGACAT TAGTCTGGAG
    AGACTGAAGT CATCTGTGAA AGCTGTCATT CTGAAGGACT CTAAAGTCTT CCCACTGCTT
    CTTTGTATAA CCCTGAATGG ACTCTGTGCT TTAGGCAGAG AACATTCATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu25942
    Clone ID Related Accession (Same CDS sequence) NM_004403.3 , XM_024446670.1 , NM_004403.2 , NM_001127453.1 , NM_001127453.2
    Accession Version NM_001127453.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1491bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-11-25
    Organism Homo sapiens(human)
    Product gasdermin-E isoform a
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA820184.1, BC125065.1 and AF131765.1. This sequence is a reference standard in the RefSeqGene project. On May 31, 2019 this sequence version replaced NM_001127453.1. Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 and 2 both encode isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.124413.1, SRR3476690.493329.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000645220.1/ ENSP00000494186.1 RefSeq Select criteria :: based on manual assertion, conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGTTTGCCA AAGCAACCAG GAATTTTCTT AGAGAAGTTG ATGCTGATGG TGACCTGATT 
    GCAGTATCAA ATCTGAATGA CTCTGATAAG TTACAGCTTC TAAGTCTGGT GACAAAAAAG
    AAGAGATTCT GGTGCTGGCA GAGACCCAAG TACCAGTTTT TATCCCTCAC CCTTGGCGAT
    GTACTCATAG AAGACCAATT TCCGAGTCCA GTGGTCGTGG AGTCGGACTT TGTGAAATAC
    GAGGGCAAGT TTGCAAACCA CGTGAGTGGA ACCCTGGAGA CTGCACTGGG GAAGGTCAAG
    CTGAACCTGG GGGGCAGCAG CCGCGTAGAG AGCCAGTCTT CATTTGGAAC CCTGAGGAAG
    CAGGAGGTGG ATTTGCAGCA GCTCATCAGA GACTCTGCCG AGAGAACAAT AAATCTGAGA
    AACCCTGTGC TCCAGCAGGT GCTGGAAGGA AGGAATGAGG TCCTGTGCGT TTTGACACAG
    AAGATCACGA CGATGCAGAA GTGTGTGATC TCTGAGCACA TGCAGGTCGA GGAGAAGTGT
    GGTGGCATCG TGGGCATCCA GACCAAGACG GTGCAGGTGT CAGCGACGGA GGATGGGAAT
    GTCACCAAGG ACTCCAACGT GGTGCTGGAG ATCCCAGCTG CCACCACCAT TGCCTACGGT
    GTCATTGAGT TATACGTGAA ACTGGACGGC CAGTTCGAGT TCTGCCTTCT CCGAGGGAAG
    CAAGGTGGCT TCGAGAACAA GAAGAGAATT GACTCTGTCT ACCTGGACCC CCTGGTCTTT
    CGAGAGTTTG CATTCATAGA CATGCCAGAT GCTGCGCATG GGATATCTTC CCAGGATGGA
    CCATTAAGTG TTTTAAAGCA AGCGACCCTG CTCCTGGAGA GGAATTTCCA TCCATTTGCG
    GAGCTGCCTG AGCCACAACA GACAGCTTTG AGTGACATCT TCCAGGCGGT CCTATTTGAT
    GATGAACTAC TCATGGTCCT GGAACCAGTG TGCGATGACC TGGTCAGCGG CCTCTCGCCC
    ACAGTGGCGG TGCTGGGGGA GCTGAAGCCC CGGCAGCAGC AGGACCTTGT GGCCTTCCTG
    CAGCTGGTGG GGTGCAGCTT ACAGGGTGGG TGTCCGGGCC CCGAGGATGC AGGCAGCAAG
    CAGCTGTTTA TGACAGCCTA CTTCTTGGTC AGTGCCCTCG CAGAAATGCC AGATAGCGCA
    GCAGCTCTGC TGGGCACTTG CTGCAAACTC CAGATCATTC CCACACTGTG CCACTTGCTT
    CGTGCTCTGT CTGATGATGG AGTATCTGAT CTTGAAGACC CAACCTTGAC TCCCCTGAAA
    GATACAGAAA GGTTTGGGAT TGTGCAGCGC TTGTTTGCCT CAGCTGACAT TAGTCTGGAG
    AGACTGAAGT CATCTGTGAA AGCTGTCATT CTGAAGGACT CTAAAGTCTT CCCACTGCTT
    CTTTGTATAA CCCTGAATGG ACTCTGTGCT TTAGGCAGAG AACATTCATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001120925.1
    CDS89..1579
    Translation

    Target ORF information:

    RefSeq Version NM_001127453.2
    Organism Homo sapiens(human)
    Definition Homo sapiens gasdermin E (GSDME), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001127453.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGTTTGCCA AAGCAACCAG GAATTTTCTT AGAGAAGTTG ATGCTGATGG TGACCTGATT 
    GCAGTATCAA ATCTGAATGA CTCTGATAAG TTACAGCTTC TAAGTCTGGT GACAAAAAAG
    AAGAGATTCT GGTGCTGGCA GAGACCCAAG TACCAGTTTT TATCCCTCAC CCTTGGCGAT
    GTACTCATAG AAGACCAATT TCCGAGTCCA GTGGTCGTGG AGTCGGACTT TGTGAAATAC
    GAGGGCAAGT TTGCAAACCA CGTGAGTGGA ACCCTGGAGA CTGCACTGGG GAAGGTCAAG
    CTGAACCTGG GGGGCAGCAG CCGCGTAGAG AGCCAGTCTT CATTTGGAAC CCTGAGGAAG
    CAGGAGGTGG ATTTGCAGCA GCTCATCAGA GACTCTGCCG AGAGAACAAT AAATCTGAGA
    AACCCTGTGC TCCAGCAGGT GCTGGAAGGA AGGAATGAGG TCCTGTGCGT TTTGACACAG
    AAGATCACGA CGATGCAGAA GTGTGTGATC TCTGAGCACA TGCAGGTCGA GGAGAAGTGT
    GGTGGCATCG TGGGCATCCA GACCAAGACG GTGCAGGTGT CAGCGACGGA GGATGGGAAT
    GTCACCAAGG ACTCCAACGT GGTGCTGGAG ATCCCAGCTG CCACCACCAT TGCCTACGGT
    GTCATTGAGT TATACGTGAA ACTGGACGGC CAGTTCGAGT TCTGCCTTCT CCGAGGGAAG
    CAAGGTGGCT TCGAGAACAA GAAGAGAATT GACTCTGTCT ACCTGGACCC CCTGGTCTTT
    CGAGAGTTTG CATTCATAGA CATGCCAGAT GCTGCGCATG GGATATCTTC CCAGGATGGA
    CCATTAAGTG TTTTAAAGCA AGCGACCCTG CTCCTGGAGA GGAATTTCCA TCCATTTGCG
    GAGCTGCCTG AGCCACAACA GACAGCTTTG AGTGACATCT TCCAGGCGGT CCTATTTGAT
    GATGAACTAC TCATGGTCCT GGAACCAGTG TGCGATGACC TGGTCAGCGG CCTCTCGCCC
    ACAGTGGCGG TGCTGGGGGA GCTGAAGCCC CGGCAGCAGC AGGACCTTGT GGCCTTCCTG
    CAGCTGGTGG GGTGCAGCTT ACAGGGTGGG TGTCCGGGCC CCGAGGATGC AGGCAGCAAG
    CAGCTGTTTA TGACAGCCTA CTTCTTGGTC AGTGCCCTCG CAGAAATGCC AGATAGCGCA
    GCAGCTCTGC TGGGCACTTG CTGCAAACTC CAGATCATTC CCACACTGTG CCACTTGCTT
    CGTGCTCTGT CTGATGATGG AGTATCTGAT CTTGAAGACC CAACCTTGAC TCCCCTGAAA
    GATACAGAAA GGTTTGGGAT TGTGCAGCGC TTGTTTGCCT CAGCTGACAT TAGTCTGGAG
    AGACTGAAGT CATCTGTGAA AGCTGTCATT CTGAAGGACT CTAAAGTCTT CCCACTGCTT
    CTTTGTATAA CCCTGAATGG ACTCTGTGCT TTAGGCAGAG AACATTCATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu26331
    Clone ID Related Accession (Same CDS sequence) XM_017011802.1 , NM_001127454.1 , NM_001127454.2
    Accession Version NM_001127454.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 999bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-12-08
    Organism Homo sapiens(human)
    Product gasdermin-E isoform b
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA908548.1, DA197867.1, BC125066.1, BC125065.1 and AF131765.1. Transcript Variant: This variant (3) lacks an alternate exon compared to variant 1, resulting in an isoform (b) that uses a downstream start site compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.170886.1, SRR1803613.169648.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGCAGAAGT GTGTGATCTC TGAGCACATG CAGGTCGAGG AGAAGTGTGG TGGCATCGTG 
    GGCATCCAGA CCAAGACGGT GCAGGTGTCA GCGACGGAGG ATGGGAATGT CACCAAGGAC
    TCCAACGTGG TGCTGGAGAT CCCAGCTGCC ACCACCATTG CCTACGGTGT CATTGAGTTA
    TACGTGAAAC TGGACGGCCA GTTCGAGTTC TGCCTTCTCC GAGGGAAGCA AGGTGGCTTC
    GAGAACAAGA AGAGAATTGA CTCTGTCTAC CTGGACCCCC TGGTCTTTCG AGAGTTTGCA
    TTCATAGACA TGCCAGATGC TGCGCATGGG ATATCTTCCC AGGATGGACC ATTAAGTGTT
    TTAAAGCAAG CGACCCTGCT CCTGGAGAGG AATTTCCATC CATTTGCGGA GCTGCCTGAG
    CCACAACAGA CAGCTTTGAG TGACATCTTC CAGGCGGTCC TATTTGATGA TGAACTACTC
    ATGGTCCTGG AACCAGTGTG CGATGACCTG GTCAGCGGCC TCTCGCCCAC AGTGGCGGTG
    CTGGGGGAGC TGAAGCCCCG GCAGCAGCAG GACCTTGTGG CCTTCCTGCA GCTGGTGGGG
    TGCAGCTTAC AGGGTGGGTG TCCGGGCCCC GAGGATGCAG GCAGCAAGCA GCTGTTTATG
    ACAGCCTACT TCTTGGTCAG TGCCCTCGCA GAAATGCCAG ATAGCGCAGC AGCTCTGCTG
    GGCACTTGCT GCAAACTCCA GATCATTCCC ACACTGTGCC ACTTGCTTCG TGCTCTGTCT
    GATGATGGAG TATCTGATCT TGAAGACCCA ACCTTGACTC CCCTGAAAGA TACAGAAAGG
    TTTGGGATTG TGCAGCGCTT GTTTGCCTCA GCTGACATTA GTCTGGAGAG ACTGAAGTCA
    TCTGTGAAAG CTGTCATTCT GAAGGACTCT AAAGTCTTCC CACTGCTTCT TTGTATAACC
    CTGAATGGAC TCTGTGCTTT AGGCAGAGAA CATTCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001120926.1
    CDS596..1594
    Translation

    Target ORF information:

    RefSeq Version NM_001127454.1
    Organism Homo sapiens(human)
    Definition Homo sapiens gasdermin E (GSDME), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001127454.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGCAGAAGT GTGTGATCTC TGAGCACATG CAGGTCGAGG AGAAGTGTGG TGGCATCGTG 
    GGCATCCAGA CCAAGACGGT GCAGGTGTCA GCGACGGAGG ATGGGAATGT CACCAAGGAC
    TCCAACGTGG TGCTGGAGAT CCCAGCTGCC ACCACCATTG CCTACGGTGT CATTGAGTTA
    TACGTGAAAC TGGACGGCCA GTTCGAGTTC TGCCTTCTCC GAGGGAAGCA AGGTGGCTTC
    GAGAACAAGA AGAGAATTGA CTCTGTCTAC CTGGACCCCC TGGTCTTTCG AGAGTTTGCA
    TTCATAGACA TGCCAGATGC TGCGCATGGG ATATCTTCCC AGGATGGACC ATTAAGTGTT
    TTAAAGCAAG CGACCCTGCT CCTGGAGAGG AATTTCCATC CATTTGCGGA GCTGCCTGAG
    CCACAACAGA CAGCTTTGAG TGACATCTTC CAGGCGGTCC TATTTGATGA TGAACTACTC
    ATGGTCCTGG AACCAGTGTG CGATGACCTG GTCAGCGGCC TCTCGCCCAC AGTGGCGGTG
    CTGGGGGAGC TGAAGCCCCG GCAGCAGCAG GACCTTGTGG CCTTCCTGCA GCTGGTGGGG
    TGCAGCTTAC AGGGTGGGTG TCCGGGCCCC GAGGATGCAG GCAGCAAGCA GCTGTTTATG
    ACAGCCTACT TCTTGGTCAG TGCCCTCGCA GAAATGCCAG ATAGCGCAGC AGCTCTGCTG
    GGCACTTGCT GCAAACTCCA GATCATTCCC ACACTGTGCC ACTTGCTTCG TGCTCTGTCT
    GATGATGGAG TATCTGATCT TGAAGACCCA ACCTTGACTC CCCTGAAAGA TACAGAAAGG
    TTTGGGATTG TGCAGCGCTT GTTTGCCTCA GCTGACATTA GTCTGGAGAG ACTGAAGTCA
    TCTGTGAAAG CTGTCATTCT GAAGGACTCT AAAGTCTTCC CACTGCTTCT TTGTATAACC
    CTGAATGGAC TCTGTGCTTT AGGCAGAGAA CATTCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu26331
    Clone ID Related Accession (Same CDS sequence) XM_017011802.1 , NM_001127454.1 , NM_001127454.2
    Accession Version NM_001127454.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 999bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-11-25
    Organism Homo sapiens(human)
    Product gasdermin-E isoform b
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA908548.1, DA197867.1, BC125066.1, BC125065.1 and AF131765.1. On Jun 1, 2019 this sequence version replaced NM_001127454.1. Transcript Variant: This variant (3) lacks an alternate exon compared to variant 1, resulting in an isoform (b) that uses a downstream start site compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.170886.1, SRR1803613.281346.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGCAGAAGT GTGTGATCTC TGAGCACATG CAGGTCGAGG AGAAGTGTGG TGGCATCGTG 
    GGCATCCAGA CCAAGACGGT GCAGGTGTCA GCGACGGAGG ATGGGAATGT CACCAAGGAC
    TCCAACGTGG TGCTGGAGAT CCCAGCTGCC ACCACCATTG CCTACGGTGT CATTGAGTTA
    TACGTGAAAC TGGACGGCCA GTTCGAGTTC TGCCTTCTCC GAGGGAAGCA AGGTGGCTTC
    GAGAACAAGA AGAGAATTGA CTCTGTCTAC CTGGACCCCC TGGTCTTTCG AGAGTTTGCA
    TTCATAGACA TGCCAGATGC TGCGCATGGG ATATCTTCCC AGGATGGACC ATTAAGTGTT
    TTAAAGCAAG CGACCCTGCT CCTGGAGAGG AATTTCCATC CATTTGCGGA GCTGCCTGAG
    CCACAACAGA CAGCTTTGAG TGACATCTTC CAGGCGGTCC TATTTGATGA TGAACTACTC
    ATGGTCCTGG AACCAGTGTG CGATGACCTG GTCAGCGGCC TCTCGCCCAC AGTGGCGGTG
    CTGGGGGAGC TGAAGCCCCG GCAGCAGCAG GACCTTGTGG CCTTCCTGCA GCTGGTGGGG
    TGCAGCTTAC AGGGTGGGTG TCCGGGCCCC GAGGATGCAG GCAGCAAGCA GCTGTTTATG
    ACAGCCTACT TCTTGGTCAG TGCCCTCGCA GAAATGCCAG ATAGCGCAGC AGCTCTGCTG
    GGCACTTGCT GCAAACTCCA GATCATTCCC ACACTGTGCC ACTTGCTTCG TGCTCTGTCT
    GATGATGGAG TATCTGATCT TGAAGACCCA ACCTTGACTC CCCTGAAAGA TACAGAAAGG
    TTTGGGATTG TGCAGCGCTT GTTTGCCTCA GCTGACATTA GTCTGGAGAG ACTGAAGTCA
    TCTGTGAAAG CTGTCATTCT GAAGGACTCT AAAGTCTTCC CACTGCTTCT TTGTATAACC
    CTGAATGGAC TCTGTGCTTT AGGCAGAGAA CATTCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001120926.1
    CDS515..1513
    Translation

    Target ORF information:

    RefSeq Version NM_001127454.2
    Organism Homo sapiens(human)
    Definition Homo sapiens gasdermin E (GSDME), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001127454.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGCAGAAGT GTGTGATCTC TGAGCACATG CAGGTCGAGG AGAAGTGTGG TGGCATCGTG 
    GGCATCCAGA CCAAGACGGT GCAGGTGTCA GCGACGGAGG ATGGGAATGT CACCAAGGAC
    TCCAACGTGG TGCTGGAGAT CCCAGCTGCC ACCACCATTG CCTACGGTGT CATTGAGTTA
    TACGTGAAAC TGGACGGCCA GTTCGAGTTC TGCCTTCTCC GAGGGAAGCA AGGTGGCTTC
    GAGAACAAGA AGAGAATTGA CTCTGTCTAC CTGGACCCCC TGGTCTTTCG AGAGTTTGCA
    TTCATAGACA TGCCAGATGC TGCGCATGGG ATATCTTCCC AGGATGGACC ATTAAGTGTT
    TTAAAGCAAG CGACCCTGCT CCTGGAGAGG AATTTCCATC CATTTGCGGA GCTGCCTGAG
    CCACAACAGA CAGCTTTGAG TGACATCTTC CAGGCGGTCC TATTTGATGA TGAACTACTC
    ATGGTCCTGG AACCAGTGTG CGATGACCTG GTCAGCGGCC TCTCGCCCAC AGTGGCGGTG
    CTGGGGGAGC TGAAGCCCCG GCAGCAGCAG GACCTTGTGG CCTTCCTGCA GCTGGTGGGG
    TGCAGCTTAC AGGGTGGGTG TCCGGGCCCC GAGGATGCAG GCAGCAAGCA GCTGTTTATG
    ACAGCCTACT TCTTGGTCAG TGCCCTCGCA GAAATGCCAG ATAGCGCAGC AGCTCTGCTG
    GGCACTTGCT GCAAACTCCA GATCATTCCC ACACTGTGCC ACTTGCTTCG TGCTCTGTCT
    GATGATGGAG TATCTGATCT TGAAGACCCA ACCTTGACTC CCCTGAAAGA TACAGAAAGG
    TTTGGGATTG TGCAGCGCTT GTTTGCCTCA GCTGACATTA GTCTGGAGAG ACTGAAGTCA
    TCTGTGAAAG CTGTCATTCT GAAGGACTCT AAAGTCTTCC CACTGCTTCT TTGTATAACC
    CTGAATGGAC TCTGTGCTTT AGGCAGAGAA CATTCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu25942
    Clone ID Related Accession (Same CDS sequence) NM_004403.3 , XM_024446670.1 , NM_004403.2 , NM_001127453.1 , NM_001127453.2
    Accession Version XM_024446670.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1491bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product gasdermin-E isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGTTTGCCA AAGCAACCAG GAATTTTCTT AGAGAAGTTG ATGCTGATGG TGACCTGATT 
    GCAGTATCAA ATCTGAATGA CTCTGATAAG TTACAGCTTC TAAGTCTGGT GACAAAAAAG
    AAGAGATTCT GGTGCTGGCA GAGACCCAAG TACCAGTTTT TATCCCTCAC CCTTGGCGAT
    GTACTCATAG AAGACCAATT TCCGAGTCCA GTGGTCGTGG AGTCGGACTT TGTGAAATAC
    GAGGGCAAGT TTGCAAACCA CGTGAGTGGA ACCCTGGAGA CTGCACTGGG GAAGGTCAAG
    CTGAACCTGG GGGGCAGCAG CCGCGTAGAG AGCCAGTCTT CATTTGGAAC CCTGAGGAAG
    CAGGAGGTGG ATTTGCAGCA GCTCATCAGA GACTCTGCCG AGAGAACAAT AAATCTGAGA
    AACCCTGTGC TCCAGCAGGT GCTGGAAGGA AGGAATGAGG TCCTGTGCGT TTTGACACAG
    AAGATCACGA CGATGCAGAA GTGTGTGATC TCTGAGCACA TGCAGGTCGA GGAGAAGTGT
    GGTGGCATCG TGGGCATCCA GACCAAGACG GTGCAGGTGT CAGCGACGGA GGATGGGAAT
    GTCACCAAGG ACTCCAACGT GGTGCTGGAG ATCCCAGCTG CCACCACCAT TGCCTACGGT
    GTCATTGAGT TATACGTGAA ACTGGACGGC CAGTTCGAGT TCTGCCTTCT CCGAGGGAAG
    CAAGGTGGCT TCGAGAACAA GAAGAGAATT GACTCTGTCT ACCTGGACCC CCTGGTCTTT
    CGAGAGTTTG CATTCATAGA CATGCCAGAT GCTGCGCATG GGATATCTTC CCAGGATGGA
    CCATTAAGTG TTTTAAAGCA AGCGACCCTG CTCCTGGAGA GGAATTTCCA TCCATTTGCG
    GAGCTGCCTG AGCCACAACA GACAGCTTTG AGTGACATCT TCCAGGCGGT CCTATTTGAT
    GATGAACTAC TCATGGTCCT GGAACCAGTG TGCGATGACC TGGTCAGCGG CCTCTCGCCC
    ACAGTGGCGG TGCTGGGGGA GCTGAAGCCC CGGCAGCAGC AGGACCTTGT GGCCTTCCTG
    CAGCTGGTGG GGTGCAGCTT ACAGGGTGGG TGTCCGGGCC CCGAGGATGC AGGCAGCAAG
    CAGCTGTTTA TGACAGCCTA CTTCTTGGTC AGTGCCCTCG CAGAAATGCC AGATAGCGCA
    GCAGCTCTGC TGGGCACTTG CTGCAAACTC CAGATCATTC CCACACTGTG CCACTTGCTT
    CGTGCTCTGT CTGATGATGG AGTATCTGAT CTTGAAGACC CAACCTTGAC TCCCCTGAAA
    GATACAGAAA GGTTTGGGAT TGTGCAGCGC TTGTTTGCCT CAGCTGACAT TAGTCTGGAG
    AGACTGAAGT CATCTGTGAA AGCTGTCATT CTGAAGGACT CTAAAGTCTT CCCACTGCTT
    CTTTGTATAA CCCTGAATGG ACTCTGTGCT TTAGGCAGAG AACATTCATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024302438.1
    CDS236..1726
    Translation

    Target ORF information:

    RefSeq Version XM_024446670.1
    Organism Homo sapiens(human)
    Definition Homo sapiens gasdermin E (GSDME), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024446670.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGTTTGCCA AAGCAACCAG GAATTTTCTT AGAGAAGTTG ATGCTGATGG TGACCTGATT 
    GCAGTATCAA ATCTGAATGA CTCTGATAAG TTACAGCTTC TAAGTCTGGT GACAAAAAAG
    AAGAGATTCT GGTGCTGGCA GAGACCCAAG TACCAGTTTT TATCCCTCAC CCTTGGCGAT
    GTACTCATAG AAGACCAATT TCCGAGTCCA GTGGTCGTGG AGTCGGACTT TGTGAAATAC
    GAGGGCAAGT TTGCAAACCA CGTGAGTGGA ACCCTGGAGA CTGCACTGGG GAAGGTCAAG
    CTGAACCTGG GGGGCAGCAG CCGCGTAGAG AGCCAGTCTT CATTTGGAAC CCTGAGGAAG
    CAGGAGGTGG ATTTGCAGCA GCTCATCAGA GACTCTGCCG AGAGAACAAT AAATCTGAGA
    AACCCTGTGC TCCAGCAGGT GCTGGAAGGA AGGAATGAGG TCCTGTGCGT TTTGACACAG
    AAGATCACGA CGATGCAGAA GTGTGTGATC TCTGAGCACA TGCAGGTCGA GGAGAAGTGT
    GGTGGCATCG TGGGCATCCA GACCAAGACG GTGCAGGTGT CAGCGACGGA GGATGGGAAT
    GTCACCAAGG ACTCCAACGT GGTGCTGGAG ATCCCAGCTG CCACCACCAT TGCCTACGGT
    GTCATTGAGT TATACGTGAA ACTGGACGGC CAGTTCGAGT TCTGCCTTCT CCGAGGGAAG
    CAAGGTGGCT TCGAGAACAA GAAGAGAATT GACTCTGTCT ACCTGGACCC CCTGGTCTTT
    CGAGAGTTTG CATTCATAGA CATGCCAGAT GCTGCGCATG GGATATCTTC CCAGGATGGA
    CCATTAAGTG TTTTAAAGCA AGCGACCCTG CTCCTGGAGA GGAATTTCCA TCCATTTGCG
    GAGCTGCCTG AGCCACAACA GACAGCTTTG AGTGACATCT TCCAGGCGGT CCTATTTGAT
    GATGAACTAC TCATGGTCCT GGAACCAGTG TGCGATGACC TGGTCAGCGG CCTCTCGCCC
    ACAGTGGCGG TGCTGGGGGA GCTGAAGCCC CGGCAGCAGC AGGACCTTGT GGCCTTCCTG
    CAGCTGGTGG GGTGCAGCTT ACAGGGTGGG TGTCCGGGCC CCGAGGATGC AGGCAGCAAG
    CAGCTGTTTA TGACAGCCTA CTTCTTGGTC AGTGCCCTCG CAGAAATGCC AGATAGCGCA
    GCAGCTCTGC TGGGCACTTG CTGCAAACTC CAGATCATTC CCACACTGTG CCACTTGCTT
    CGTGCTCTGT CTGATGATGG AGTATCTGAT CTTGAAGACC CAACCTTGAC TCCCCTGAAA
    GATACAGAAA GGTTTGGGAT TGTGCAGCGC TTGTTTGCCT CAGCTGACAT TAGTCTGGAG
    AGACTGAAGT CATCTGTGAA AGCTGTCATT CTGAAGGACT CTAAAGTCTT CCCACTGCTT
    CTTTGTATAA CCCTGAATGG ACTCTGTGCT TTAGGCAGAG AACATTCATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu26331
    Clone ID Related Accession (Same CDS sequence) XM_017011802.1 , NM_001127454.1 , NM_001127454.2
    Accession Version XM_017011802.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 999bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product gasdermin-E isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGCAGAAGT GTGTGATCTC TGAGCACATG CAGGTCGAGG AGAAGTGTGG TGGCATCGTG 
    GGCATCCAGA CCAAGACGGT GCAGGTGTCA GCGACGGAGG ATGGGAATGT CACCAAGGAC
    TCCAACGTGG TGCTGGAGAT CCCAGCTGCC ACCACCATTG CCTACGGTGT CATTGAGTTA
    TACGTGAAAC TGGACGGCCA GTTCGAGTTC TGCCTTCTCC GAGGGAAGCA AGGTGGCTTC
    GAGAACAAGA AGAGAATTGA CTCTGTCTAC CTGGACCCCC TGGTCTTTCG AGAGTTTGCA
    TTCATAGACA TGCCAGATGC TGCGCATGGG ATATCTTCCC AGGATGGACC ATTAAGTGTT
    TTAAAGCAAG CGACCCTGCT CCTGGAGAGG AATTTCCATC CATTTGCGGA GCTGCCTGAG
    CCACAACAGA CAGCTTTGAG TGACATCTTC CAGGCGGTCC TATTTGATGA TGAACTACTC
    ATGGTCCTGG AACCAGTGTG CGATGACCTG GTCAGCGGCC TCTCGCCCAC AGTGGCGGTG
    CTGGGGGAGC TGAAGCCCCG GCAGCAGCAG GACCTTGTGG CCTTCCTGCA GCTGGTGGGG
    TGCAGCTTAC AGGGTGGGTG TCCGGGCCCC GAGGATGCAG GCAGCAAGCA GCTGTTTATG
    ACAGCCTACT TCTTGGTCAG TGCCCTCGCA GAAATGCCAG ATAGCGCAGC AGCTCTGCTG
    GGCACTTGCT GCAAACTCCA GATCATTCCC ACACTGTGCC ACTTGCTTCG TGCTCTGTCT
    GATGATGGAG TATCTGATCT TGAAGACCCA ACCTTGACTC CCCTGAAAGA TACAGAAAGG
    TTTGGGATTG TGCAGCGCTT GTTTGCCTCA GCTGACATTA GTCTGGAGAG ACTGAAGTCA
    TCTGTGAAAG CTGTCATTCT GAAGGACTCT AAAGTCTTCC CACTGCTTCT TTGTATAACC
    CTGAATGGAC TCTGTGCTTT AGGCAGAGAA CATTCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016867291.1
    CDS352..1350
    Translation

    Target ORF information:

    RefSeq Version XM_017011802.1
    Organism Homo sapiens(human)
    Definition Homo sapiens gasdermin E (GSDME), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017011802.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGCAGAAGT GTGTGATCTC TGAGCACATG CAGGTCGAGG AGAAGTGTGG TGGCATCGTG 
    GGCATCCAGA CCAAGACGGT GCAGGTGTCA GCGACGGAGG ATGGGAATGT CACCAAGGAC
    TCCAACGTGG TGCTGGAGAT CCCAGCTGCC ACCACCATTG CCTACGGTGT CATTGAGTTA
    TACGTGAAAC TGGACGGCCA GTTCGAGTTC TGCCTTCTCC GAGGGAAGCA AGGTGGCTTC
    GAGAACAAGA AGAGAATTGA CTCTGTCTAC CTGGACCCCC TGGTCTTTCG AGAGTTTGCA
    TTCATAGACA TGCCAGATGC TGCGCATGGG ATATCTTCCC AGGATGGACC ATTAAGTGTT
    TTAAAGCAAG CGACCCTGCT CCTGGAGAGG AATTTCCATC CATTTGCGGA GCTGCCTGAG
    CCACAACAGA CAGCTTTGAG TGACATCTTC CAGGCGGTCC TATTTGATGA TGAACTACTC
    ATGGTCCTGG AACCAGTGTG CGATGACCTG GTCAGCGGCC TCTCGCCCAC AGTGGCGGTG
    CTGGGGGAGC TGAAGCCCCG GCAGCAGCAG GACCTTGTGG CCTTCCTGCA GCTGGTGGGG
    TGCAGCTTAC AGGGTGGGTG TCCGGGCCCC GAGGATGCAG GCAGCAAGCA GCTGTTTATG
    ACAGCCTACT TCTTGGTCAG TGCCCTCGCA GAAATGCCAG ATAGCGCAGC AGCTCTGCTG
    GGCACTTGCT GCAAACTCCA GATCATTCCC ACACTGTGCC ACTTGCTTCG TGCTCTGTCT
    GATGATGGAG TATCTGATCT TGAAGACCCA ACCTTGACTC CCCTGAAAGA TACAGAAAGG
    TTTGGGATTG TGCAGCGCTT GTTTGCCTCA GCTGACATTA GTCTGGAGAG ACTGAAGTCA
    TCTGTGAAAG CTGTCATTCT GAAGGACTCT AAAGTCTTCC CACTGCTTCT TTGTATAACC
    CTGAATGGAC TCTGTGCTTT AGGCAGAGAA CATTCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.