DEFA5 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	DEFA5
Entrez Gene ID	1670
Full Name	defensin alpha 5
Synonyms	DEF5,HD-5
General protein information	Preferred Names defensin alpha 5 Names defensin-5 HD5(20-94) defensin, alpha 5, Paneth cell-specific defensin, alpha 5, preproprotein
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 8 Map Location: 8p23.1
Summary	Defensins are a family of antimicrobial and cytotoxic peptides thought to be involved in host defense. They are abundant in the granules of neutrophils and also found in the epithelia of mucosal surfaces such as those of the intestine, respiratory tract, urinary tract, and vagina. Members of the defensin family are highly similar in protein sequence and distinguished by a conserved cysteine motif. Several of the alpha defensin genes appear to be clustered on chromosome 8. The protein encoded by this gene, defensin, alpha 5, is highly expressed in the secretory granules of Paneth cells of the ileum. [provided by RefSeq, Oct 2014].
Disorder MIM:	600472

mRNA and Protein(s)

mRNA	Protein	Name
NM_021010.3	NP_066290.1	defensin-5 preproprotein
NM_021010.2	NP_066290.1	defensin-5 preproprotein

Pathways from BioSystems

Homologies

	LOC574310	NP_001028094.1
Homo sapiens (human)	DEFA5	NP_066290.1
Pan troglodytes (chimpanzee)	DEFA5	NP_001012657.1
	DEFA4	NP_001028095.1
	LOC695005	XP_001083971.1
	LOC574309	NP_001028093.1
	LOC574383	NP_001028130.1
	MNP2	NP_001028035.1
	LOC574382	NP_001028129.1

Gene Ontology
Bibliography

Related articles in PubMed

Alpha-defensins (?-Defs) in Crohn's disease: decrease of ileal ?-Def 5 via permanent methylation and increase in plasma ?-Def 1-3 concentrations offering biomarker utility.
Cerrillo E, Moret I, Iborra M, Ramos D, Bus? E, Tortosa L, S?ez-Gonz?lez E, Nos P, Beltr?n B
Clinical and experimental immunology(2017 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Different dynamics and pathway of disulfide bonds reduction has been demonstrated for two human defensins, HD5 and HBD-3.
Title: Different dynamics and pathway of disulfide bonds reduction of two human defensins, a molecular dynamics simulation study.

Human adenovirus 3 produces large amounts of penton-dodecahedra (PtDd), virus-like particles, during replication, which also function in blocking of HD5.
Title: Studies on the Interaction of Tumor-Derived HD5 Alpha Defensins with Adenoviruses and Implications for Oncolytic Adenovirus Therapy.

Human alpha-Defensin-5 is a potential candidate biomarker to molecularly differentiate Crohn's colitis from ulcerative colitis, to our knowledge. These data give us both a potential diagnostic marker in Human alpha-Defensin-5 and insight to develop future mechanistic studies to better understand crypt biology in Crohn's colitis.
Title: Human alpha defensin 5 is a candidate biomarker to delineate inflammatory bowel disease.

These data support capsid stabilization and redirection to the lysosome during infection as a general antiviral mechanism of alpha-defensins against nonenveloped viruses.
Title: ?-Defensin HD5 Inhibits Human Papillomavirus 16 Infection via Capsid Stabilization and Redirection to the Lysosome.

no biologic differences between wild-type HD5 and its variants were observed on HD5 antibacterial activity against Escherichia coli
Title: Prediction of the impact of coding missense and nonsense single nucleotide polymorphisms on HD5 and HBD1 antibacterial activity against Escherichia coli.

The following DEFA5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DEFA5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu15135	NM_021010.3 Latest version!	Homo sapiens defensin alpha 5 (DEFA5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
View Old Accession Versions >>
OHu15135	NM_021010.2	Homo sapiens defensin alpha 5 (DEFA5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DEFA5 ( NM_021010.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_066290.1 Homo sapiens defensin alpha 5 (DEFA5), mRNA.

Price & Availability↑

CloneID	OHu15135
Clone ID Related Accession (Same CDS sequence)	NM_021010.3 , NM_021010.2
Accession Version	NM_021010.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 285bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-22
Organism	Homo sapiens(human)
Product	defensin-5 preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BM854660.1 and BP419449.1. On Nov 22, 2018 this sequence version replaced NM_021010.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BM854660.1, BP424870.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000330590.4/ ENSP00000329890.2 Protein has antimicrobial activity :: PMID: 15616305 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGAGGACCA TCGCCATCCT TGCTGCCATT CTCCTGGTGG CCCTGCAGGC CCAGGCTGAG 
TCACTCCAGG AAAGAGCTGA TGAGGCTACA ACCCAGAAGC AGTCTGGGGA AGACAACCAG 
GACCTTGCTA TCTCCTTTGC AGGAAATGGA CTCTCTGCTC TTAGAACCTC AGGTTCTCAG 
GCAAGAGCCA CCTGCTATTG CCGAACCGGC CGTTGTGCTA CCCGTGAGTC CCTCTCCGGG 
GTGTGTGAAA TCAGTGGCCG CCTCTACAGA CTCTGCTGTC GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_066290.1
CDS	43..327
Translation	MRTIAILAAILLVALQAQAESLQERADEATTQKQSGEDNQDLAISFAGNGLSALRTSGSQARATCYCRTGRCATRESLSGVCEISGRLYRLCCR

Target ORF information:

RefSeq Version	NM_021010.3
Organism	Homo sapiens(human)
Definition	Homo sapiens defensin alpha 5 (DEFA5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_021010.3

ORF Insert Sequence:

ATGAGGACCA TCGCCATCCT TGCTGCCATT CTCCTGGTGG CCCTGCAGGC CCAGGCTGAG 
TCACTCCAGG AAAGAGCTGA TGAGGCTACA ACCCAGAAGC AGTCTGGGGA AGACAACCAG 
GACCTTGCTA TCTCCTTTGC AGGAAATGGA CTCTCTGCTC TTAGAACCTC AGGTTCTCAG 
GCAAGAGCCA CCTGCTATTG CCGAACCGGC CGTTGTGCTA CCCGTGAGTC CCTCTCCGGG 
GTGTGTGAAA TCAGTGGCCG CCTCTACAGA CTCTGCTGTC GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DEFA5 ( NM_021010.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_066290.1 Homo sapiens defensin alpha 5 (DEFA5), mRNA.

Price & Availability↑

CloneID	OHu15135
Clone ID Related Accession (Same CDS sequence)	NM_021010.3 , NM_021010.2
Accession Version	NM_021010.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 285bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-22
Organism	Homo sapiens(human)
Product	defensin-5 preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BM854660.1 and BP419449.1. On Oct 10, 2014 this sequence version replaced NM_021010.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BM854660.1, BP424870.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## Protein has antimicrobial activity :: PMID: 15616305 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGAGGACCA TCGCCATCCT TGCTGCCATT CTCCTGGTGG CCCTGCAGGC CCAGGCTGAG 
TCACTCCAGG AAAGAGCTGA TGAGGCTACA ACCCAGAAGC AGTCTGGGGA AGACAACCAG 
GACCTTGCTA TCTCCTTTGC AGGAAATGGA CTCTCTGCTC TTAGAACCTC AGGTTCTCAG 
GCAAGAGCCA CCTGCTATTG CCGAACCGGC CGTTGTGCTA CCCGTGAGTC CCTCTCCGGG 
GTGTGTGAAA TCAGTGGCCG CCTCTACAGA CTCTGCTGTC GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_066290.1
CDS	43..327
Translation	MRTIAILAAILLVALQAQAESLQERADEATTQKQSGEDNQDLAISFAGNGLSALRTSGSQARATCYCRTGRCATRESLSGVCEISGRLYRLCCR

Target ORF information:

RefSeq Version	NM_021010.2
Organism	Homo sapiens(human)
Definition	Homo sapiens defensin alpha 5 (DEFA5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_021010.2

ORF Insert Sequence:

ATGAGGACCA TCGCCATCCT TGCTGCCATT CTCCTGGTGG CCCTGCAGGC CCAGGCTGAG 
TCACTCCAGG AAAGAGCTGA TGAGGCTACA ACCCAGAAGC AGTCTGGGGA AGACAACCAG 
GACCTTGCTA TCTCCTTTGC AGGAAATGGA CTCTCTGCTC TTAGAACCTC AGGTTCTCAG 
GCAAGAGCCA CCTGCTATTG CCGAACCGGC CGTTGTGCTA CCCGTGAGTC CCTCTCCGGG 
GTGTGTGAAA TCAGTGGCCG CCTCTACAGA CTCTGCTGTC GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Alpha-defensins (?-Defs) in Crohn's disease: decrease of ileal ?-Def 5 via permanent methylation and increase in plasma ?-Def 1-3 concentrations offering biomarker utility.
Clinical and experimental immunology(2017 Nov)
Cerrillo E,Moret I,Iborra M,Ramos D,Bus? E,Tortosa L,S?ez-Gonz?lez E,Nos P,Beltr?n B

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DEFA5 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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