DEFA1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	DEFA1
Entrez Gene ID	1667
Full Name	defensin alpha 1
Synonyms	DEF1,DEFA2,HNP-1,HP-1,HP1,MRS
General protein information	Preferred Names defensin alpha 1 Names neutrophil defensin 1 defensin, alpha 1, myeloid-related sequence defensin, alpha 2 myeloid-related sequence
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 8 Map Location: 8p23.1
Summary	Defensins are a family of antimicrobial and cytotoxic peptides thought to be involved in host defense. They are abundant in the granules of neutrophils and also found in the epithelia of mucosal surfaces such as those of the intestine, respiratory tract, urinary tract, and vagina. Members of the defensin family are highly similar in protein sequence and distinguished by a conserved cysteine motif. The protein encoded by this gene, defensin, alpha 1, is found in the microbicidal granules of neutrophils and likely plays a role in phagocyte-mediated host defense. Several alpha defensin genes are clustered on chromosome 8. This gene differs from defensin, alpha 3 by only one amino acid. This gene and the gene encoding defensin, alpha 3 are both subject to copy number variation. [provided by RefSeq, Oct 2014].
Disorder MIM:	125220

mRNA and Protein(s)

mRNA	Protein	Name
XM_011534740.2	XP_011533042.1	neutrophil defensin 1 isoform X1
XM_011534740.3	XP_011533042.1	neutrophil defensin 1 isoform X1
NM_004084.3	NP_004075.1	neutrophil defensin 1 preproprotein

Pathways from BioSystems

Homologies

Homo sapiens (human)	DEFA1B	NP_001035965.1
Homo sapiens (human)	DEFA1	NP_004075.1
Homo sapiens (human)	DEFA3	NP_005208.1

Gene Ontology
Bibliography

Related articles in PubMed

PPBP and DEFA1/DEFA3 genes in hyperlipidaemia as feasible synergistic inflammatory biomarkers for coronary heart disease.
Maneerat Y, Prasongsukarn K, Benjathummarak S, Dechkhajorn W
Lipids in health and disease16(1)80(2017 Apr)

Glycoproteomics Reveals Decorin Peptides With Anti-Myostatin Activity in Human Atrial Fibrillation.
Barallobre-Barreiro J, Gupta SK, Zoccarato A, Kitazume-Taneike R, Fava M, Yin X, Werner T, Hirt MN, Zampetaki A, Viviano A, Chong M, Bern M, Kourliouros A, Domenech N, Willeit P, Shah AM, Jahangiri M, Schaefer L, Fischer JW, Iozzo RV, Viner R, Thum T, Heineke J, Kichler A, Otsu K, Mayr M
Circulation134(11)817-32(2016 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

these results demonstrate that HNPs1-3 may be potent inhibitors of ADAMTS13 activity, likely by binding to the central A2 domain of VWF and physically blocking ADAMTS13 binding.
Title: Human neutrophil peptides inhibit cleavage of von Willebrand factor by ADAMTS13: a potential link of inflammation to TTP.

HNP1 upregulation of cytokine expression in pDCs was inhibited by blockade of NF-kappaB activation or knockdown of IRF1, demonstrating the importance of these two signaling events in HNP1-induced pDC activation.
Title: Alarmin human ? defensin HNP1 activates plasmacytoid dendritic cells by triggering NF-?B and IRF1 signaling pathways.

Increased alpha-defensin expression is associated with risk of coronary heart disease in hyperlipidemia patients.
Title: Increased alpha-defensin expression is associated with risk of coronary heart disease: a feasible predictive inflammatory biomarker of coronary heart disease in hyperlipidemia patients.

Levels in sera and CSF were elevated in Alzheimer disease patients compared with controls.
Title: Relevance of defensin ?-2 and ? defensins (HNP1-3) in Alzheimer's disease.

HNP1 functions as a "molecular brake" on macrophage-driven inflammation, ensuring both pathogen clearance and the resolution of inflammation with minimal bystander tissue damage.
Title: Neutrophil-derived alpha defensins control inflammation by inhibiting macrophage mRNA translation.

The following DEFA1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DEFA1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu12997	NM_004084.3 Latest version!	Homo sapiens defensin alpha 1 (DEFA1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu57229	XM_011534740.3 Latest version!	Homo sapiens defensin alpha 1 (DEFA1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
View Old Accession Versions >>
OHu57229	XM_011534740.2	Homo sapiens defensin alpha 1 (DEFA1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DEFA1 ( NM_004084.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_004075.1 Homo sapiens defensin alpha 1 (DEFA1), mRNA.

Price & Availability↑

CloneID	OHu12997
Clone ID Related Accession (Same CDS sequence)	NM_004084.3
Accession Version	NM_004084.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 285bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-07
Organism	Homo sapiens(human)
Product	neutrophil defensin 1 preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL036554.2, M26602.1 and AW291977.1. On May 13, 2010 this sequence version replaced NM_004084.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##RefSeq-Attributes-START## Protein has antimicrobial activity :: PMID: 2997278 RefSeq Select criteria :: based on expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGAGGACCC TCGCCATCCT TGCTGCCATT CTCCTGGTGG CCCTGCAGGC CCAGGCTGAG 
CCACTCCAGG CAAGAGCTGA TGAGGTTGCT GCAGCCCCGG AGCAGATTGC AGCGGACATC 
CCAGAAGTGG TTGTTTCCCT TGCATGGGAC GAAAGCTTGG CTCCAAAGCA TCCAGGCTCA 
AGGAAAAACA TGGCCTGCTA TTGCAGAATA CCAGCGTGCA TTGCAGGAGA ACGTCGCTAT 
GGAACCTGCA TCTACCAGGG AAGACTCTGG GCATTCTGCT GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004075.1
CDS	93..377
Translation	MRTLAILAAILLVALQAQAEPLQARADEVAAAPEQIAADIPEVVVSLAWDESLAPKHPGSRKNMACYCRIPACIAGERRYGTCIYQGRLWAFCC

Target ORF information:

RefSeq Version	NM_004084.3
Organism	Homo sapiens(human)
Definition	Homo sapiens defensin alpha 1 (DEFA1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004084.3

ORF Insert Sequence:

ATGAGGACCC TCGCCATCCT TGCTGCCATT CTCCTGGTGG CCCTGCAGGC CCAGGCTGAG 
CCACTCCAGG CAAGAGCTGA TGAGGTTGCT GCAGCCCCGG AGCAGATTGC AGCGGACATC 
CCAGAAGTGG TTGTTTCCCT TGCATGGGAC GAAAGCTTGG CTCCAAAGCA TCCAGGCTCA 
AGGAAAAACA TGGCCTGCTA TTGCAGAATA CCAGCGTGCA TTGCAGGAGA ACGTCGCTAT 
GGAACCTGCA TCTACCAGGG AAGACTCTGG GCATTCTGCT GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DEFA1 ( XM_011534740.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011533042.1 Homo sapiens defensin alpha 1 (DEFA1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu57229
Clone ID Related Accession (Same CDS sequence)	XM_011534740.2 , XM_011534740.3
Accession Version	XM_011534740.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 306bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	neutrophil defensin 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000008.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011534740.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGATGG TGACCCCAGC CATGAGGACC CTCGCCATCC TTGCTGCCAT TCTCCTGGTG 
GCCCTGCAGG CCCAGGCTGA GCCACTCCAG GCAAGAGCTG ATGAGGTTGC TGCAGCCCCG 
GAGCAGATTG CAGCGGACAT CCCAGAAGTG GTTGTTTCCC TTGCATGGGA CGAAAGCTTG 
GCTCCAAAGC ATCCAGGCTC AAGGAAAAAC ATGGCCTGCT ATTGCAGAAT ACCAGCGTGC 
ATTGCAGGAG AACGTCGCTA TGGAACCTGC ATCTACCAGG GAAGACTCTG GGCATTCTGC 
TGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011533042.1
CDS	143..448
Translation	MRMVTPAMRTLAILAAILLVALQAQAEPLQARADEVAAAPEQIAADIPEVVVSLAWDESLAPKHPGSRKNMACYCRIPACIAGERRYGTCIYQGRLWAFCC

Target ORF information:

RefSeq Version	XM_011534740.2
Organism	Homo sapiens(human)
Definition	Homo sapiens defensin alpha 1 (DEFA1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011534740.2

ORF Insert Sequence:

ATGAGGATGG TGACCCCAGC CATGAGGACC CTCGCCATCC TTGCTGCCAT TCTCCTGGTG 
GCCCTGCAGG CCCAGGCTGA GCCACTCCAG GCAAGAGCTG ATGAGGTTGC TGCAGCCCCG 
GAGCAGATTG CAGCGGACAT CCCAGAAGTG GTTGTTTCCC TTGCATGGGA CGAAAGCTTG 
GCTCCAAAGC ATCCAGGCTC AAGGAAAAAC ATGGCCTGCT ATTGCAGAAT ACCAGCGTGC 
ATTGCAGGAG AACGTCGCTA TGGAACCTGC ATCTACCAGG GAAGACTCTG GGCATTCTGC 
TGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DEFA1 ( XM_011534740.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011533042.1 Homo sapiens defensin alpha 1 (DEFA1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu57229
Clone ID Related Accession (Same CDS sequence)	XM_011534740.2 , XM_011534740.3
Accession Version	XM_011534740.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 306bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	neutrophil defensin 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000008.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011534740.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGATGG TGACCCCAGC CATGAGGACC CTCGCCATCC TTGCTGCCAT TCTCCTGGTG 
GCCCTGCAGG CCCAGGCTGA GCCACTCCAG GCAAGAGCTG ATGAGGTTGC TGCAGCCCCG 
GAGCAGATTG CAGCGGACAT CCCAGAAGTG GTTGTTTCCC TTGCATGGGA CGAAAGCTTG 
GCTCCAAAGC ATCCAGGCTC AAGGAAAAAC ATGGCCTGCT ATTGCAGAAT ACCAGCGTGC 
ATTGCAGGAG AACGTCGCTA TGGAACCTGC ATCTACCAGG GAAGACTCTG GGCATTCTGC 
TGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011533042.1
CDS	144..449
Translation	MRMVTPAMRTLAILAAILLVALQAQAEPLQARADEVAAAPEQIAADIPEVVVSLAWDESLAPKHPGSRKNMACYCRIPACIAGERRYGTCIYQGRLWAFCC

Target ORF information:

RefSeq Version	XM_011534740.3
Organism	Homo sapiens(human)
Definition	Homo sapiens defensin alpha 1 (DEFA1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011534740.3

ORF Insert Sequence:

ATGAGGATGG TGACCCCAGC CATGAGGACC CTCGCCATCC TTGCTGCCAT TCTCCTGGTG 
GCCCTGCAGG CCCAGGCTGA GCCACTCCAG GCAAGAGCTG ATGAGGTTGC TGCAGCCCCG 
GAGCAGATTG CAGCGGACAT CCCAGAAGTG GTTGTTTCCC TTGCATGGGA CGAAAGCTTG 
GCTCCAAAGC ATCCAGGCTC AAGGAAAAAC ATGGCCTGCT ATTGCAGAAT ACCAGCGTGC 
ATTGCAGGAG AACGTCGCTA TGGAACCTGC ATCTACCAGG GAAGACTCTG GGCATTCTGC 
TGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

PPBP and DEFA1/DEFA3 genes in hyperlipidaemia as feasible synergistic inflammatory biomarkers for coronary heart disease.
Lipids in health and disease16(1)80(2017 Apr)
Maneerat Y,Prasongsukarn K,Benjathummarak S,Dechkhajorn W

Glycoproteomics Reveals Decorin Peptides With Anti-Myostatin Activity in Human Atrial Fibrillation.
Circulation134(11)817-32(2016 Sep)
Barallobre-Barreiro J,Gupta SK,Zoccarato A,Kitazume-Taneike R,Fava M,Yin X,Werner T,Hirt MN,Zampetaki A,Viviano A,Chong M,Bern M,Kourliouros A,Domenech N,Willeit P,Shah AM,Jahangiri M,Schaefer L,Fischer JW,Iozzo RV,Viner R,Thum T,Heineke J,Kichler A,Otsu K,Mayr M

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DEFA1 cDNA ORF clone, Homo sapiens(human)

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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