DDT cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	DDT
Entrez Gene ID	1652
Full Name	D-dopachrome tautomerase
Synonyms	DDCT
General protein information	Preferred Names D-dopachrome tautomerase Names D-dopachrome decarboxylase phenylpyruvate tautomerase II
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 22 Map Location: 22q11.23
Summary	D-dopachrome tautomerase converts D-dopachrome into 5,6-dihydroxyindole. The DDT gene is related to the migration inhibitory factor (MIF) in terms of sequence, enzyme activity, and gene structure. DDT and MIF are closely linked on chromosome 22. [provided by RefSeq, Jul 2008].
Disorder MIM:	602750

mRNA and Protein(s)

mRNA	Protein	Name
NM_001355.3	NP_001346.1	D-dopachrome decarboxylase
NM_001084392.1	NP_001077861.1	D-dopachrome decarboxylase
NM_001355.4	NP_001346.1	D-dopachrome decarboxylase

Pathways from BioSystems

Homologies

Homo sapiens (human)	DDT	NP_001346.1
Canis lupus familiaris (dog)	LOC100856501	XP_003639975.1
Bos taurus (cattle)	DDT	NP_001092620.1
Danio rerio (zebrafish)	ddt	NP_001002147.1
Xenopus tropicalis (tropical clawed frog)	ddt	NP_001017261.1
	DDT	XP_001087658.1
Mus musculus (house mouse)	Ddt	NP_034157.1
Gallus gallus (chicken)	DDT	NP_001025838.1
Rattus norvegicus (Norway rat)	Ddt	NP_077045.1

Gene Ontology
Bibliography

Related articles in PubMed

The AMPK/mTOR pathway is involved in D-dopachrome tautomerase gene transcription in adipocytes differentiated from SGBS cells, a human preadipocyte cell line.
Iwata T, Kuribayashi K, Nakasono M, Saito-Tarashima N, Minakawa N, Mizusawa N, Kido R, Yoshimoto K
Cytokine96195-202(2017 Aug)

Combined Knockdown of D-dopachrome Tautomerase and Migration Inhibitory Factor Inhibits the Proliferation, Migration, and Invasion in Human Cervical Cancer.
Wang Q, Wei Y, Zhang J
International journal of gynecological cancer : official journal of the International Gynecological Cancer Society27(4)634-642(2017 May)

Modeling of both shared and distinct interactions between MIF and its homologue D-DT with their common receptor CD74.
Meza-Romero R, Benedek G, Jordan K, Leng L, Pantouris G, Lolis E, Bucala R, Vandenbark AA
Cytokine8862-70(2016 Dec)

D-dopachrome tautomerase is over-expressed in pancreatic ductal adenocarcinoma and acts cooperatively with macrophage migration inhibitory factor to promote cancer growth.
Guo D, Guo J, Yao J, Jiang K, Hu J, Wang B, Liu H, Lin L, Sun W, Jiang X
International journal of cancer139(9)2056-67(2016 11)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The knockdown of D-DT and MIF, individually and additively, inhibited the proliferation, migration, and invasion in HeLa and SiHa cells and restrained the growth of xenograft tumor.
Title: Combined Knockdown of D-dopachrome Tautomerase and Migration Inhibitory Factor Inhibits the Proliferation, Migration, and Invasion in Human Cervical Cancer.

These results have implications for the manner in which D-DT and MIF compete with each other for binding to the CD74 receptor and for the relative potency of DRa1-MOG-35-55 and RTL1000 for competitive inhibition of D-DT and MIF binding and activation through CD74.
Title: Modeling of both shared and distinct interactions between MIF and its homologue D-DT with their common receptor CD74.

Study demonstrated that DDT was over- expressed in pancreatic ductal adenocarcinoma (PDAC) tissues and cell lines in a pattern correlated with MIF, and knockdown of DDT and MIF in PANC- 1 cells cooperatively inhibited cell proliferation, invasion and tumor formation. The tautomerase activities of both MIF and DDT are required for their negative regulatory role in p53 and their tumor-promoting functions.
Title: D-dopachrome tautomerase is over-expressed in pancreatic ductal adenocarcinoma and acts cooperatively with macrophage migration inhibitory factor to promote cancer growth.

DDT was increased in burn patients.
Title: The clinical significance of the MIF homolog d-dopachrome tautomerase (MIF-2) and its circulating receptor (sCD74) in burn.

Gene expression level of DDT is significantly higher in AD patients when compared to normal controls.
Title: Association of gene expression and methylation of UQCRC1 to the predisposition of Alzheimer's disease in a Chinese population.

The following DDT gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DDT cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu15894	NM_001355.4 Latest version!	Homo sapiens D-dopachrome tautomerase (DDT), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu15894	NM_001084392.1 Latest version!	Homo sapiens D-dopachrome tautomerase (DDT), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
View Old Accession Versions >>
OHu15894	NM_001355.3	Homo sapiens D-dopachrome tautomerase (DDT), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DDT ( NM_001355.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001346.1 Homo sapiens D-dopachrome tautomerase (DDT), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu15894
Clone ID Related Accession (Same CDS sequence)	NM_001355.3 , NM_001084392.1 , NM_001355.4
Accession Version	NM_001355.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 357bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-16
Organism	Homo sapiens(human)
Product	D-dopachrome decarboxylase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from U49785.1, AP000351.3 and CA414359.1. On Apr 19, 2007 this sequence version replaced NM_001355.2. Transcript Variant: This variant (1) represents the longer transcript. Variants 1 and 2 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U49785.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1968189, SAMEA1968540 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCGTTCC TGGAGCTGGA CACGAATTTG CCCGCCAACC GAGTGCCCGC GGGGCTGGAG 
AAACGACTCT GCGCCGCCGC TGCCTCCATC CTGGGCAAAC CTGCGGACCG CGTGAACGTG 
ACGGTACGGC CGGGCCTGGC CATGGCGCTG AGCGGGTCCA CCGAGCCCTG CGCGCAGCTG 
TCCATCTCCT CCATCGGCGT AGTGGGCACC GCCGAGGACA ACCGCAGCCA CAGCGCCCAC 
TTCTTTGAGT TTCTCACCAA GGAGCTAGCC CTGGGCCAGG ACCGGATACT TATCCGCTTT 
TTCCCCTTGG AGTCCTGGCA GATTGGCAAG ATAGGGACGG TCATGACTTT TTTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001346.1
CDS	129..485
Translation	MPFLELDTNLPANRVPAGLEKRLCAAAASILGKPADRVNVTVRPGLAMALSGSTEPCAQLSISSIGVVGTAEDNRSHSAHFFEFLTKELALGQDRILIRFFPLESWQIGKIGTVMTFL

Target ORF information:

RefSeq Version	NM_001355.3
Organism	Homo sapiens(human)
Definition	Homo sapiens D-dopachrome tautomerase (DDT), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001355.3

ORF Insert Sequence:

ATGCCGTTCC TGGAGCTGGA CACGAATTTG CCCGCCAACC GAGTGCCCGC GGGGCTGGAG 
AAACGACTCT GCGCCGCCGC TGCCTCCATC CTGGGCAAAC CTGCGGACCG CGTGAACGTG 
ACGGTACGGC CGGGCCTGGC CATGGCGCTG AGCGGGTCCA CCGAGCCCTG CGCGCAGCTG 
TCCATCTCCT CCATCGGCGT AGTGGGCACC GCCGAGGACA ACCGCAGCCA CAGCGCCCAC 
TTCTTTGAGT TTCTCACCAA GGAGCTAGCC CTGGGCCAGG ACCGGATACT TATCCGCTTT 
TTCCCCTTGG AGTCCTGGCA GATTGGCAAG ATAGGGACGG TCATGACTTT TTTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DDT ( NM_001084392.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001077861.1 Homo sapiens D-dopachrome tautomerase (DDT), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu15894
Clone ID Related Accession (Same CDS sequence)	NM_001355.3 , NM_001084392.1 , NM_001355.4
Accession Version	NM_001084392.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 357bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Homo sapiens(human)
Product	D-dopachrome decarboxylase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AP000351.3, BC015508.2 and CA414359.1. Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 and 2 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BQ928788.1, SRR1163657.51601.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCCGTTCC TGGAGCTGGA CACGAATTTG CCCGCCAACC GAGTGCCCGC GGGGCTGGAG 
AAACGACTCT GCGCCGCCGC TGCCTCCATC CTGGGCAAAC CTGCGGACCG CGTGAACGTG 
ACGGTACGGC CGGGCCTGGC CATGGCGCTG AGCGGGTCCA CCGAGCCCTG CGCGCAGCTG 
TCCATCTCCT CCATCGGCGT AGTGGGCACC GCCGAGGACA ACCGCAGCCA CAGCGCCCAC 
TTCTTTGAGT TTCTCACCAA GGAGCTAGCC CTGGGCCAGG ACCGGATACT TATCCGCTTT 
TTCCCCTTGG AGTCCTGGCA GATTGGCAAG ATAGGGACGG TCATGACTTT TTTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001077861.1
CDS	78..434
Translation	MPFLELDTNLPANRVPAGLEKRLCAAAASILGKPADRVNVTVRPGLAMALSGSTEPCAQLSISSIGVVGTAEDNRSHSAHFFEFLTKELALGQDRILIRFFPLESWQIGKIGTVMTFL

Target ORF information:

RefSeq Version	NM_001084392.1
Organism	Homo sapiens(human)
Definition	Homo sapiens D-dopachrome tautomerase (DDT), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001084392.1

ORF Insert Sequence:

ATGCCGTTCC TGGAGCTGGA CACGAATTTG CCCGCCAACC GAGTGCCCGC GGGGCTGGAG 
AAACGACTCT GCGCCGCCGC TGCCTCCATC CTGGGCAAAC CTGCGGACCG CGTGAACGTG 
ACGGTACGGC CGGGCCTGGC CATGGCGCTG AGCGGGTCCA CCGAGCCCTG CGCGCAGCTG 
TCCATCTCCT CCATCGGCGT AGTGGGCACC GCCGAGGACA ACCGCAGCCA CAGCGCCCAC 
TTCTTTGAGT TTCTCACCAA GGAGCTAGCC CTGGGCCAGG ACCGGATACT TATCCGCTTT 
TTCCCCTTGG AGTCCTGGCA GATTGGCAAG ATAGGGACGG TCATGACTTT TTTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DDT ( NM_001355.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_001346.1 Homo sapiens D-dopachrome tautomerase (DDT), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu15894
Clone ID Related Accession (Same CDS sequence)	NM_001355.3 , NM_001084392.1 , NM_001355.4
Accession Version	NM_001355.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 357bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-03
Organism	Homo sapiens(human)
Product	D-dopachrome decarboxylase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from U49785.1, AP000351.3 and CA414359.1. On Jun 2, 2019 this sequence version replaced NM_001355.3. Transcript Variant: This variant (1) represents the longer transcript. Variants 1 and 2 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U49785.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1968189, SAMEA1968540 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCGTTCC TGGAGCTGGA CACGAATTTG CCCGCCAACC GAGTGCCCGC GGGGCTGGAG 
AAACGACTCT GCGCCGCCGC TGCCTCCATC CTGGGCAAAC CTGCGGACCG CGTGAACGTG 
ACGGTACGGC CGGGCCTGGC CATGGCGCTG AGCGGGTCCA CCGAGCCCTG CGCGCAGCTG 
TCCATCTCCT CCATCGGCGT AGTGGGCACC GCCGAGGACA ACCGCAGCCA CAGCGCCCAC 
TTCTTTGAGT TTCTCACCAA GGAGCTAGCC CTGGGCCAGG ACCGGATACT TATCCGCTTT 
TTCCCCTTGG AGTCCTGGCA GATTGGCAAG ATAGGGACGG TCATGACTTT TTTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001346.1
CDS	129..485
Translation	MPFLELDTNLPANRVPAGLEKRLCAAAASILGKPADRVNVTVRPGLAMALSGSTEPCAQLSISSIGVVGTAEDNRSHSAHFFEFLTKELALGQDRILIRFFPLESWQIGKIGTVMTFL

Target ORF information:

RefSeq Version	NM_001355.4
Organism	Homo sapiens(human)
Definition	Homo sapiens D-dopachrome tautomerase (DDT), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001355.4

ORF Insert Sequence:

ATGCCGTTCC TGGAGCTGGA CACGAATTTG CCCGCCAACC GAGTGCCCGC GGGGCTGGAG 
AAACGACTCT GCGCCGCCGC TGCCTCCATC CTGGGCAAAC CTGCGGACCG CGTGAACGTG 
ACGGTACGGC CGGGCCTGGC CATGGCGCTG AGCGGGTCCA CCGAGCCCTG CGCGCAGCTG 
TCCATCTCCT CCATCGGCGT AGTGGGCACC GCCGAGGACA ACCGCAGCCA CAGCGCCCAC 
TTCTTTGAGT TTCTCACCAA GGAGCTAGCC CTGGGCCAGG ACCGGATACT TATCCGCTTT 
TTCCCCTTGG AGTCCTGGCA GATTGGCAAG ATAGGGACGG TCATGACTTT TTTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The AMPK/mTOR pathway is involved in D-dopachrome tautomerase gene transcription in adipocytes differentiated from SGBS cells, a human preadipocyte cell line.
Cytokine96195-202(2017 Aug)
Iwata T,Kuribayashi K,Nakasono M,Saito-Tarashima N,Minakawa N,Mizusawa N,Kido R,Yoshimoto K

Combined Knockdown of D-dopachrome Tautomerase and Migration Inhibitory Factor Inhibits the Proliferation, Migration, and Invasion in Human Cervical Cancer.
International journal of gynecological cancer : official journal of the International Gynecological Cancer Society27(4)634-642(2017 May)
Wang Q,Wei Y,Zhang J

Modeling of both shared and distinct interactions between MIF and its homologue D-DT with their common receptor CD74.
Cytokine8862-70(2016 Dec)
Meza-Romero R,Benedek G,Jordan K,Leng L,Pantouris G,Lolis E,Bucala R,Vandenbark AA

D-dopachrome tautomerase is over-expressed in pancreatic ductal adenocarcinoma and acts cooperatively with macrophage migration inhibitory factor to promote cancer growth.
International journal of cancer139(9)2056-67(2016 11)
Guo D,Guo J,Yao J,Jiang K,Hu J,Wang B,Liu H,Lin L,Sun W,Jiang X

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DDT cDNA ORF clone, Homo sapiens(human)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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