DCTN1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	DCTN1
Entrez Gene ID	1639
Full Name	dynactin subunit 1
Synonyms	DAP-150,DP-150,P135
General protein information	Preferred Names dynactin subunit 1 Names dynactin subunit 1 150 kDa dynein-associated polypeptide dynactin 1 (p150, glued homolog, Drosophila)
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 2 Map Location: 2p13.1
Summary	This gene encodes the largest subunit of dynactin, a macromolecular complex consisting of 10 subunits ranging in size from 22 to 150 kD. Dynactin binds to both microtubules and cytoplasmic dynein. Dynactin is involved in a diverse array of cellular functions, including ER-to-Golgi transport, the centripetal movement of lysosomes and endosomes, spindle formation, chromosome movement, nuclear positioning, and axonogenesis. This subunit interacts with dynein intermediate chain by its domains directly binding to dynein and binds to microtubules via a highly conserved glycine-rich cytoskeleton-associated protein (CAP-Gly) domain in its N-terminus. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms. Mutations in this gene cause distal hereditary motor neuronopathy type VIIB (HMN7B) which is also known as distal spinal and bulbar muscular atrophy (dSBMA). [provided by RefSeq, Oct 2008].
Disorder MIM:	601143
Disorder Html:	Neuropathy, distal hereditary motor, type VIIB, 607641 (3); {Amyotrophic lateral sclerosis, susceptibility to}, 105400 (3); Perry syndrome, 168605 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001190836.1	NP_001177765.1	dynactin subunit 1 isoform 5
NM_001135040.2	NP_001128512.1	dynactin subunit 1 isoform 3
NM_023019.3	NP_075408.1	dynactin subunit 1 isoform 2
NM_001135041.2	NP_001128513.1	dynactin subunit 1 isoform 4
NM_004082.4	NP_004073.2	dynactin subunit 1 isoform 1
NM_001190837.1	NP_001177766.1	dynactin subunit 1 isoform 6

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	DCTN1	XP_005630593.1
Xenopus tropicalis (tropical clawed frog)	dctn1	XP_002936330.2
Pan troglodytes (chimpanzee)	DCTN1	XP_001156535.1
Mus musculus (house mouse)	Dctn1	NP_031861.2
Gallus gallus (chicken)	DCTN1	NP_001026538.1
Danio rerio (zebrafish)	dctn1b	XP_001342673.3
Bos taurus (cattle)	DCTN1	NP_001092404.1
Drosophila melanogaster (fruit fly)	Gl	NP_524061.1
Homo sapiens (human)	DCTN1	NP_004073.2
	DCTN1	XP_001108486.2
Rattus norvegicus (Norway rat)	Dctn1	NP_077044.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004194	XP_003436738.1

Gene Ontology
Bibliography

Related articles in PubMed

Characterization of functional domains of human EB1 family proteins.
Bu W, Su LK
The Journal of biological chemistry278(50)49721-31(2003 Dec)

Evidence that an interaction between EB1 and p150(Glued) is required for the formation and maintenance of a radial microtubule array anchored at the centrosome.
Askham JM, Vaughan KT, Goodson HV, Morrison EE
Molecular biology of the cell13(10)3627-45(2002 Oct)

Genomic organization of the DCTN1-SLC4A5 locus encoding both NBC4 and p150(Glued).
Pushkin A, Abuladze N, Newman D, Tatishchev S, Kurtz I
Cytogenetics and cell genetics95(3-4)163-8(2001)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Results suggested that variants in DCTN1 are not common risk factors for Chinese sporadic amyotrophic lateral sclerosis and that the frequency of variants of unknown significance in the cohort study was 0.39%.
Title: DCTN1 gene analysis in Chinese patients with sporadic amyotrophic lateral sclerosis.

Mutations in the Dynactin 1 (DCTN1) gene have been demonstrated to result in various neurodegenerative diseases, including distal hereditary motor neuropathy type 7B. In the present study, a p.G59S mutation was identified as the underlying cause of Distal hereditary motor neuropathy type 7B in two families, and their detailed clinical features were characterized.
Title: Distal hereditary motor neuropathy type?7B with Dynactin 1 mutation.

No DCTN1 variants of disease significance were identified in this study suggesting the dynactin genes are unlikely to be a common cause of inherited peripheral neuropathies.
Title: Mutation analysis of genes within the dynactin complex in a cohort of hereditary peripheral neuropathies.

This work reveals the structural details of Hook3's interaction with dynein and offers insight into how cargo adaptors form processive dynein-dynactin motor complexes.
Title: Assembly and activation of dynein-dynactin by the cargo adaptor protein Hook3.

Study found that in peripheral blood mononuclear cells the median expression of KIFC3, KIF1B, and KIF5C was much lower than the expression of dynactin subunits DCTN1 and DCTN3, in both sporadic amyotrophic lateral sclerosis and healthy cases
Title: Alteration of Motor Protein Expression Involved in Bidirectional Transport in Peripheral Blood Mononuclear Cells of Patients with Amyotrophic Lateral Sclerosis.

The following DCTN1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DCTN1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu18647	NM_004082.4 Latest version!	Homo sapiens dynactin subunit 1 (DCTN1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$459.50-$643.30 $919.00
OHu16693	NM_001190836.1 Latest version!	Homo sapiens dynactin subunit 1 (DCTN1), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu16697	NM_001135041.2 Latest version!	Homo sapiens dynactin subunit 1 (DCTN1), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OHu16728	NM_023019.3 Latest version!	Homo sapiens dynactin subunit 1 (DCTN1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OHu16796	NM_001135040.2 Latest version!	Homo sapiens dynactin subunit 1 (DCTN1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu16952	NM_001190837.1 Latest version!	Homo sapiens dynactin subunit 1 (DCTN1), transcript variant 6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DCTN1 ( NM_004082.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_004073.2 Homo sapiens dynactin subunit 1 (DCTN1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu18647
Clone ID Related Accession (Same CDS sequence)	NM_004082.4
Accession Version	NM_004082.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3837bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Homo sapiens(human)
Product	dynactin subunit 1 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA315227.1, BX640799.1, BC013978.2, X98801.1 and CA448857.1. This sequence is a reference standard in the RefSeqGene project. On Jul 1, 2010 this sequence version replaced NM_004082.3. Transcript Variant: This variant (1) has multiple differences in the 5' UTR, 5' coding region and 3' coding region, compared to variant 4. These differences cause translation initiation from an upstream AUG and a protein (isoform 1) with a longer N-terminus containing a CAP-Gly domain and a longer C-terminus, compared to isoform 4. Isoform 1 is also called p150. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.206908.1, SRR1803614.154823.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCACAGA GCAAGAGGCA CGTGTACAGC CGGACGCCCA GCGGCAGCAG GATGAGTGCG 
GAGGCAAGCG CCCGGCCTCT GCGGGTGGGC TCCCGTGTAG AGGTGATTGG AAAAGGCCAC 
CGAGGCACTG TGGCCTATGT TGGAGCCACA CTGTTTGCCA CTGGCAAATG GGTAGGCGTG 
ATTCTGGATG AAGCAAAGGG CAAAAATGAT GGAACTGTTC AAGGCAGGAA GTACTTCACT 
TGTGATGAAG GGCATGGCAT CTTTGTGCGC CAGTCCCAGA TCCAGGTATT TGAAGATGGA 
GCAGATACTA CTTCCCCAGA GACACCTGAT TCTTCTGCTT CAAAAGTCCT CAAAAGAGAG 
GGAACTGATA CAACTGCAAA GACTAGCAAA CTGCGGGGAC TGAAGCCTAA GAAGGCACCG 
ACAGCCCGAA AGACCACAAC TCGGCGACCC AAGCCCACGC GCCCAGCCAG TACTGGGGTG 
GCTGGGGCCA GTAGCTCCCT GGGCCCCTCT GGCTCAGCGT CAGCAGGTGA GCTGAGCAGC 
AGTGAGCCCA GCACCCCGGC TCAGACTCCG CTGGCAGCAC CCATCATCCC CACGCCGGTC 
CTCACCTCTC CTGGAGCAGT CCCCCCGCTT CCTTCCCCAT CCAAGGAGGA GGAGGGACTA 
AGGGCTCAGG TGCGGGACCT GGAGGAGAAA CTAGAGACCC TGAGACTGAA ACGGGCAGAA 
GACAAAGCAA AGCTAAAAGA GCTGGAGAAA CACAAAATCC AGCTGGAGCA GGTGCAGGAA 
TGGAAGAGCA AAATGCAGGA GCAGCAGGCC GACCTGCAGC GGCGCCTCAA GGAGGCGAGA 
AAGGAAGCCA AGGAGGCGCT GGAGGCAAAG GAACGCTATA TGGAGGAGAT GGCTGATACT 
GCTGATGCCA TTGAGATGGC CACTTTGGAC AAGGAGATGG CTGAAGAGCG GGCTGAGTCC 
CTGCAGCAGG AGGTGGAGGC ACTGAAGGAG CGGGTGGACG AGCTCACTAC TGACTTAGAG 
ATCCTCAAGG CTGAGATTGA AGAGAAGGGC TCAGATGGCG CTGCATCCAG TTATCAGCTC 
AAGCAGCTTG AGGAGCAGAA TGCCCGCCTG AAGGATGCCC TGGTGAGGAT GCGGGATCTT 
TCTTCCTCAG AGAAGCAGGA GCATGTGAAG CTCCAGAAGC TCATGGAAAA GAAGAACCAA 
GAGCTGGAAG TTGTGAGGCA ACAGCGGGAG CGTCTGCAGG AGGAGCTAAG CCAGGCAGAG 
AGCACCATTG ATGAGCTCAA GGAGCAGGTG GATGCTGCTC TGGGTGCTGA GGAGATGGTG 
GAGATGCTGA CAGATCGGAA CCTGAATCTG GAAGAGAAAG TGCGCGAGTT GAGGGAGACT 
GTGGGAGACT TGGAAGCGAT GAATGAGATG AACGATGAGC TGCAGGAGAA TGCACGTGAG 
ACAGAACTGG AGCTGCGGGA GCAGCTGGAC ATGGCAGGCG CGCGGGTTCG TGAGGCCCAG 
AAGCGTGTGG AGGCAGCCCA GGAGACGGTT GCAGACTACC AGCAGACCAT CAAGAAGTAC 
CGCCAGCTGA CCGCCCATCT ACAGGATGTG AATCGGGAAC TGACAAACCA GCAGGAAGCA 
TCTGTGGAGA GGCAACAGCA GCCACCTCCA GAGACCTTTG ACTTCAAAAT CAAGTTTGCT 
GAGACTAAGG CCCATGCCAA GGCAATTGAG ATGGAATTGA GGCAGATGGA GGTGGCCCAG 
GCCAATCGAC ACATGTCCCT GCTGACAGCC TTCATGCCTG ACAGCTTCCT TCGGCCAGGT 
GGGGACCATG ACTGCGTTCT GGTGCTGTTG CTCATGCCTC GTCTCATTTG CAAGGCAGAG 
CTGATCCGGA AGCAGGCCCA GGAGAAGTTT GAACTAAGTG AGAACTGTTC AGAGCGGCCT 
GGGCTGCGAG GAGCTGCTGG GGAGCAACTC AGCTTTGCTG CTGGACTGGT GTACTCGCTG 
AGCCTGCTGC AGGCCACGCT ACACCGCTAT GAGCATGCCC TCTCTCAGTG CAGTGTGGAT 
GTGTATAAGA AAGTGGGCAG CCTGTACCCT GAGATGAGTG CCCATGAGCG CTCCTTGGAT 
TTCCTCATTG AACTGCTGCA CAAGGATCAG CTGGATGAGA CTGTCAATGT GGAGCCTCTC 
ACCAAGGCCA TCAAGTACTA TCAGCATCTG TACAGCATCC ACCTTGCCGA ACAGCCTGAG 
GACTGTACTA TGCAGCTGGC TGACCACATT AAGTTCACGC AGAGTGCTCT GGACTGCATG 
AGTGTGGAGG TAGGACGGCT GCGTGCCTTC TTGCAGGGTG GGCAGGAGGC TACAGATATT 
GCCCTCCTGC TCCGGGATCT GGAAACTTCA TGCAGTGACA TCCGCCAGTT CTGCAAGAAG 
ATCCGAAGGC GAATGCCAGG GACAGATGCT CCTGGGATCC CAGCTGCACT GGCCTTTGGA 
CCACAGGTAT CTGACACGCT CCTAGACTGC AGGAAACACT TGACGTGGGT CGTGGCTGTG 
CTGCAGGAGG TGGCAGCTGC TGCTGCCCAG CTCATTGCCC CACTGGCAGA GAATGAGGGG 
CTACTTGTGG CTGCTCTGGA GGAACTGGCT TTCAAAGCAA GCGAGCAGAT CTATGGGACC 
CCCTCCAGCA GCCCCTATGA GTGTCTGCGC CAGTCATGCA ACATCCTCAT CAGTACCATG 
AACAAGCTGG CCACAGCCAT GCAGGAGGGG GAGTATGATG CAGAGCGGCC CCCCAGCAAG 
CCTCCACCGG TTGAACTGCG GGCTGCTGCC CTTCGTGCAG AGATCACAGA TGCTGAAGGC 
CTGGGTTTGA AGCTCGAAGA TCGAGAGACA GTTATTAAGG AGTTGAAGAA GTCACTCAAG 
ATTAAGGGAG AGGAGCTAAG TGAGGCCAAT GTGCGGCTGA GCCTCCTGGA GAAGAAGTTG 
GACAGTGCTG CCAAGGATGC AGATGAGCGC ATCGAGAAAG TCCAGACTCG GCTGGAGGAG 
ACCCAGGCAC TGCTGCGAAA GAAGGAGAAA GAGTTTGAGG AGACAATGGA TGCACTCCAG 
GCTGACATCG ACCAGCTGGA GGCAGAGAAG GCAGAACTAA AGCAGCGTCT GAACAGCCAG 
TCCAAACGCA CGATTGAGGG ACTCCGGGGC CCTCCTCCTT CAGGCATTGC TACTCTGGTC 
TCTGGCATTG CTGGTGAAGA ACAGCAGCGA GGAGCCATCC CTGGGCAGGC TCCAGGGTCT 
GTGCCAGGCC CAGGGCTGGT GAAGGACTCA CCACTGCTGC TTCAGCAGAT CTCTGCCATG 
AGGCTGCACA TCTCCCAGCT CCAGCATGAG AACAGCATCC TCAAGGGAGC CCAGATGAAG 
GCATCCTTGG CATCCCTGCC CCCTCTGCAT GTTGCAAAGC TATCCCATGA GGGCCCTGGC 
AGTGAGTTAC CAGCTGGAGC GCTGTATCGT AAGACCAGCC AGCTGCTGGA GACATTGAAT 
CAATTGAGCA CACACACGCA CGTAGTAGAC ATCACTCGCA CCAGCCCTGC TGCCAAGAGC 
CCGTCGGCCC AACTTATGGA GCAAGTGGCT CAGCTTAAGT CCCTGAGTGA CACCGTCGAG 
AAGCTCAAGG ATGAGGTCCT CAAGGAGACA GTATCTCAGC GCCCTGGAGC CACAGTACCC 
ACTGACTTTG CCACCTTCCC TTCATCAGCC TTCCTCAGGG CCAAGGAGGA GCAGCAGGAT 
GACACAGTCT ACATGGGCAA AGTGACCTTC TCATGTGCGG CTGGTTTTGG ACAGCGACAC 
CGGCTGGTGC TGACCCAGGA GCAGCTGCAC CAGCTTCACA GTCGCCTCAT CTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004073.2
CDS	319..4155
Translation	MAQSKRHVYSRTPSGSRMSAEASARPLRVGSRVEVIGKGHRGTVAYVGATLFATGKWVGVILDEAKGKNDGTVQGRKYFTCDEGHGIFVRQSQIQVFEDGADTTSPETPDSSASKVLKREGTDTTAKTSKLRGLKPKKAPTARKTTTRRPKPTRPASTGVAGASSSLGPSGSASAGELSSSEPSTPAQTPLAAPIIPTPVLTSPGAVPPLPSPSKEEEGLRAQVRDLEEKLETLRLKRAEDKAKLKELEKHKIQLEQVQEWKSKMQEQQADLQRRLKEARKEAKEALEAKERYMEEMADTADAIEMATLDKEMAEERAESLQQEVEALKERVDELTTDLEILKAEIEEKGSDGAASSYQLKQLEEQNARLKDALVRMRDLSSSEKQEHVKLQKLMEKKNQELEVVRQQRERLQEELSQAESTIDELKEQVDAALGAEEMVEMLTDRNLNLEEKVRELRETVGDLEAMNEMNDELQENARETELELREQLDMAGARVREAQKRVEAAQETVADYQQTIKKYRQLTAHLQDVNRELTNQQEASVERQQQPPPETFDFKIKFAETKAHAKAIEMELRQMEVAQANRHMSLLTAFMPDSFLRPGGDHDCVLVLLLMPRLICKAELIRKQAQEKFELSENCSERPGLRGAAGEQLSFAAGLVYSLSLLQATLHRYEHALSQCSVDVYKKVGSLYPEMSAHERSLDFLIELLHKDQLDETVNVEPLTKAIKYYQHLYSIHLAEQPEDCTMQLADHIKFTQSALDCMSVEVGRLRAFLQGGQEATDIALLLRDLETSCSDIRQFCKKIRRRMPGTDAPGIPAALAFGPQVSDTLLDCRKHLTWVVAVLQEVAAAAAQLIAPLAENEGLLVAALEELAFKASEQIYGTPSSSPYECLRQSCNILISTMNKLATAMQEGEYDAERPPSKPPPVELRAAALRAEITDAEGLGLKLEDRETVIKELKKSLKIKGEELSEANVRLSLLEKKLDSAAKDADERIEKVQTRLEETQALLRKKEKEFEETMDALQADIDQLEAEKAELKQRLNSQSKRTIEGLRGPPPSGIATLVSGIAGEEQQRGAIPGQAPGSVPGPGLVKDSPLLLQQISAMRLHISQLQHENSILKGAQMKASLASLPPLHVAKLSHEGPGSELPAGALYRKTSQLLETLNQLSTHTHVVDITRTSPAAKSPSAQLMEQVAQLKSLSDTVEKLKDEVLKETVSQRPGATVPTDFATFPSSAFLRAKEEQQDDTVYMGKVTFSCAAGFGQRHRLVLTQEQLHQLHSRLIS

Target ORF information:

RefSeq Version	NM_004082.4
Organism	Homo sapiens(human)
Definition	Homo sapiens dynactin subunit 1 (DCTN1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004082.4

ORF Insert Sequence:

ATGGCACAGA GCAAGAGGCA CGTGTACAGC CGGACGCCCA GCGGCAGCAG GATGAGTGCG 
GAGGCAAGCG CCCGGCCTCT GCGGGTGGGC TCCCGTGTAG AGGTGATTGG AAAAGGCCAC 
CGAGGCACTG TGGCCTATGT TGGAGCCACA CTGTTTGCCA CTGGCAAATG GGTAGGCGTG 
ATTCTGGATG AAGCAAAGGG CAAAAATGAT GGAACTGTTC AAGGCAGGAA GTACTTCACT 
TGTGATGAAG GGCATGGCAT CTTTGTGCGC CAGTCCCAGA TCCAGGTATT TGAAGATGGA 
GCAGATACTA CTTCCCCAGA GACACCTGAT TCTTCTGCTT CAAAAGTCCT CAAAAGAGAG 
GGAACTGATA CAACTGCAAA GACTAGCAAA CTGCGGGGAC TGAAGCCTAA GAAGGCACCG 
ACAGCCCGAA AGACCACAAC TCGGCGACCC AAGCCCACGC GCCCAGCCAG TACTGGGGTG 
GCTGGGGCCA GTAGCTCCCT GGGCCCCTCT GGCTCAGCGT CAGCAGGTGA GCTGAGCAGC 
AGTGAGCCCA GCACCCCGGC TCAGACTCCG CTGGCAGCAC CCATCATCCC CACGCCGGTC 
CTCACCTCTC CTGGAGCAGT CCCCCCGCTT CCTTCCCCAT CCAAGGAGGA GGAGGGACTA 
AGGGCTCAGG TGCGGGACCT GGAGGAGAAA CTAGAGACCC TGAGACTGAA ACGGGCAGAA 
GACAAAGCAA AGCTAAAAGA GCTGGAGAAA CACAAAATCC AGCTGGAGCA GGTGCAGGAA 
TGGAAGAGCA AAATGCAGGA GCAGCAGGCC GACCTGCAGC GGCGCCTCAA GGAGGCGAGA 
AAGGAAGCCA AGGAGGCGCT GGAGGCAAAG GAACGCTATA TGGAGGAGAT GGCTGATACT 
GCTGATGCCA TTGAGATGGC CACTTTGGAC AAGGAGATGG CTGAAGAGCG GGCTGAGTCC 
CTGCAGCAGG AGGTGGAGGC ACTGAAGGAG CGGGTGGACG AGCTCACTAC TGACTTAGAG 
ATCCTCAAGG CTGAGATTGA AGAGAAGGGC TCAGATGGCG CTGCATCCAG TTATCAGCTC 
AAGCAGCTTG AGGAGCAGAA TGCCCGCCTG AAGGATGCCC TGGTGAGGAT GCGGGATCTT 
TCTTCCTCAG AGAAGCAGGA GCATGTGAAG CTCCAGAAGC TCATGGAAAA GAAGAACCAA 
GAGCTGGAAG TTGTGAGGCA ACAGCGGGAG CGTCTGCAGG AGGAGCTAAG CCAGGCAGAG 
AGCACCATTG ATGAGCTCAA GGAGCAGGTG GATGCTGCTC TGGGTGCTGA GGAGATGGTG 
GAGATGCTGA CAGATCGGAA CCTGAATCTG GAAGAGAAAG TGCGCGAGTT GAGGGAGACT 
GTGGGAGACT TGGAAGCGAT GAATGAGATG AACGATGAGC TGCAGGAGAA TGCACGTGAG 
ACAGAACTGG AGCTGCGGGA GCAGCTGGAC ATGGCAGGCG CGCGGGTTCG TGAGGCCCAG 
AAGCGTGTGG AGGCAGCCCA GGAGACGGTT GCAGACTACC AGCAGACCAT CAAGAAGTAC 
CGCCAGCTGA CCGCCCATCT ACAGGATGTG AATCGGGAAC TGACAAACCA GCAGGAAGCA 
TCTGTGGAGA GGCAACAGCA GCCACCTCCA GAGACCTTTG ACTTCAAAAT CAAGTTTGCT 
GAGACTAAGG CCCATGCCAA GGCAATTGAG ATGGAATTGA GGCAGATGGA GGTGGCCCAG 
GCCAATCGAC ACATGTCCCT GCTGACAGCC TTCATGCCTG ACAGCTTCCT TCGGCCAGGT 
GGGGACCATG ACTGCGTTCT GGTGCTGTTG CTCATGCCTC GTCTCATTTG CAAGGCAGAG 
CTGATCCGGA AGCAGGCCCA GGAGAAGTTT GAACTAAGTG AGAACTGTTC AGAGCGGCCT 
GGGCTGCGAG GAGCTGCTGG GGAGCAACTC AGCTTTGCTG CTGGACTGGT GTACTCGCTG 
AGCCTGCTGC AGGCCACGCT ACACCGCTAT GAGCATGCCC TCTCTCAGTG CAGTGTGGAT 
GTGTATAAGA AAGTGGGCAG CCTGTACCCT GAGATGAGTG CCCATGAGCG CTCCTTGGAT 
TTCCTCATTG AACTGCTGCA CAAGGATCAG CTGGATGAGA CTGTCAATGT GGAGCCTCTC 
ACCAAGGCCA TCAAGTACTA TCAGCATCTG TACAGCATCC ACCTTGCCGA ACAGCCTGAG 
GACTGTACTA TGCAGCTGGC TGACCACATT AAGTTCACGC AGAGTGCTCT GGACTGCATG 
AGTGTGGAGG TAGGACGGCT GCGTGCCTTC TTGCAGGGTG GGCAGGAGGC TACAGATATT 
GCCCTCCTGC TCCGGGATCT GGAAACTTCA TGCAGTGACA TCCGCCAGTT CTGCAAGAAG 
ATCCGAAGGC GAATGCCAGG GACAGATGCT CCTGGGATCC CAGCTGCACT GGCCTTTGGA 
CCACAGGTAT CTGACACGCT CCTAGACTGC AGGAAACACT TGACGTGGGT CGTGGCTGTG 
CTGCAGGAGG TGGCAGCTGC TGCTGCCCAG CTCATTGCCC CACTGGCAGA GAATGAGGGG 
CTACTTGTGG CTGCTCTGGA GGAACTGGCT TTCAAAGCAA GCGAGCAGAT CTATGGGACC 
CCCTCCAGCA GCCCCTATGA GTGTCTGCGC CAGTCATGCA ACATCCTCAT CAGTACCATG 
AACAAGCTGG CCACAGCCAT GCAGGAGGGG GAGTATGATG CAGAGCGGCC CCCCAGCAAG 
CCTCCACCGG TTGAACTGCG GGCTGCTGCC CTTCGTGCAG AGATCACAGA TGCTGAAGGC 
CTGGGTTTGA AGCTCGAAGA TCGAGAGACA GTTATTAAGG AGTTGAAGAA GTCACTCAAG 
ATTAAGGGAG AGGAGCTAAG TGAGGCCAAT GTGCGGCTGA GCCTCCTGGA GAAGAAGTTG 
GACAGTGCTG CCAAGGATGC AGATGAGCGC ATCGAGAAAG TCCAGACTCG GCTGGAGGAG 
ACCCAGGCAC TGCTGCGAAA GAAGGAGAAA GAGTTTGAGG AGACAATGGA TGCACTCCAG 
GCTGACATCG ACCAGCTGGA GGCAGAGAAG GCAGAACTAA AGCAGCGTCT GAACAGCCAG 
TCCAAACGCA CGATTGAGGG ACTCCGGGGC CCTCCTCCTT CAGGCATTGC TACTCTGGTC 
TCTGGCATTG CTGGTGAAGA ACAGCAGCGA GGAGCCATCC CTGGGCAGGC TCCAGGGTCT 
GTGCCAGGCC CAGGGCTGGT GAAGGACTCA CCACTGCTGC TTCAGCAGAT CTCTGCCATG 
AGGCTGCACA TCTCCCAGCT CCAGCATGAG AACAGCATCC TCAAGGGAGC CCAGATGAAG 
GCATCCTTGG CATCCCTGCC CCCTCTGCAT GTTGCAAAGC TATCCCATGA GGGCCCTGGC 
AGTGAGTTAC CAGCTGGAGC GCTGTATCGT AAGACCAGCC AGCTGCTGGA GACATTGAAT 
CAATTGAGCA CACACACGCA CGTAGTAGAC ATCACTCGCA CCAGCCCTGC TGCCAAGAGC 
CCGTCGGCCC AACTTATGGA GCAAGTGGCT CAGCTTAAGT CCCTGAGTGA CACCGTCGAG 
AAGCTCAAGG ATGAGGTCCT CAAGGAGACA GTATCTCAGC GCCCTGGAGC CACAGTACCC 
ACTGACTTTG CCACCTTCCC TTCATCAGCC TTCCTCAGGG CCAAGGAGGA GCAGCAGGAT 
GACACAGTCT ACATGGGCAA AGTGACCTTC TCATGTGCGG CTGGTTTTGG ACAGCGACAC 
CGGCTGGTGC TGACCCAGGA GCAGCTGCAC CAGCTTCACA GTCGCCTCAT CTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DCTN1 ( NM_001190836.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001177765.1 Homo sapiens dynactin subunit 1 (DCTN1), transcript variant 5, mRNA.

Price & Availability↑

CloneID	OHu16693
Clone ID Related Accession (Same CDS sequence)	NM_001190836.1
Accession Version	NM_001190836.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3711bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-06
Organism	Homo sapiens(human)
Product	dynactin subunit 1 isoform 5
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC309469.1, BC013978.2, AK297286.1 and CA448857.1. This sequence is a reference standard in the RefSeqGene project. Transcript Variant: This variant (5) has multiple differences in the 5' end, compared to variant 4. These differences cause translation initiation from an upstream AUG and a protein (isoform 5) with a longer N-terminus, compared to isoform 4. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.177112.1, SRR1803614.2149.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

ATGAGTGCGG AGGCAAGCGC CCGGCCTCTG CGGGTGGGCT CCCGTGTAGA GGTGATTGGA 
AAAGGCCACC GAGGCACTGT GGCCTATGTT GGAGCCACAC TGTTTGCCAC TGGCAAATGG 
GTAGGCGTGA TTCTGGATGA AGCAAAGGGC AAAAATGATG GAACTGTTCA AGGCAGGAAG 
TACTTCACTT GTGATGAAGG GCATGGCATC TTTGTGCGCC AGTCCCAGAT CCAGGTATTT 
GAAGATGGAG CAGATACTAC TTCCCCAGAG ACACCTGATT CTTCTGCTTC AAAAGTCCTC 
AAAAGAGAGG GAACTGATAC AACTGCAAAG ACTAGCAAAC TGCCCACGCG CCCAGCCAGT 
ACTGGGGTGG CTGGGGCCAG TAGCTCCCTG GGCCCCTCTG GCTCAGCGTC AGCAGGTGAG 
CTGAGCAGCA GTGAGCCCAG CACCCCGGCT CAGACTCCGC TGGCAGCACC CATCATCCCC 
ACGCCGGTCC TCACCTCTCC TGGAGCAGTC CCCCCGCTTC CTTCCCCATC CAAGGAGGAG 
GAGGGACTAA GGGCTCAGGT GCGGGACCTG GAGGAGAAAC TAGAGACCCT GAGACTGAAA 
CGGGCAGAAG ACAAAGCAAA GCTAAAAGAG CTGGAGAAAC ACAAAATCCA GCTGGAGCAG 
GTGCAGGAAT GGAAGAGCAA AATGCAGGAG CAGCAGGCCG ACCTGCAGCG GCGCCTCAAG 
GAGGCGAGAA AGGAAGCCAA GGAGGCGCTG GAGGCAAAGG AACGCTATAT GGAGGAGATG 
GCTGATACTG CTGATGCCAT TGAGATGGCC ACTTTGGACA AGGAGATGGC TGAAGAGCGG 
GCTGAGTCCC TGCAGCAGGA GGTGGAGGCA CTGAAGGAGC GGGTGGACGA GCTCACTACT 
GACTTAGAGA TCCTCAAGGC TGAGATTGAA GAGAAGGGCT CAGATGGCGC TGCATCCAGT 
TATCAGCTCA AGCAGCTTGA GGAGCAGAAT GCCCGCCTGA AGGATGCCCT GGTGAGGATG 
CGGGATCTTT CTTCCTCAGA GAAGCAGGAG CATGTGAAGC TCCAGAAGCT CATGGAAAAG 
AAGAACCAAG AGCTGGAAGT TGTGAGGCAA CAGCGGGAGC GTCTGCAGGA GGAGCTAAGC 
CAGGCAGAGA GCACCATTGA TGAGCTCAAG GAGCAGGTGG ATGCTGCTCT GGGTGCTGAG 
GAGATGGTGG AGATGCTGAC AGATCGGAAC CTGAATCTGG AAGAGAAAGT GCGCGAGTTG 
AGGGAGACTG TGGGAGACTT GGAAGCGATG AATGAGATGA ACGATGAGCT GCAGGAGAAT 
GCACGTGAGA CAGAACTGGA GCTGCGGGAG CAGCTGGACA TGGCAGGCGC GCGGGTTCGT 
GAGGCCCAGA AGCGTGTGGA GGCAGCCCAG GAGACGGTTG CAGACTACCA GCAGACCATC 
AAGAAGTACC GCCAGCTGAC CGCCCATCTA CAGGATGTGA ATCGGGAACT GACAAACCAG 
CAGGAAGCAT CTGTGGAGAG GCAACAGCAG CCACCTCCAG AGACCTTTGA CTTCAAAATC 
AAGTTTGCTG AGACTAAGGC CCATGCCAAG GCAATTGAGA TGGAATTGAG GCAGATGGAG 
GTGGCCCAGG CCAATCGACA CATGTCCCTG CTGACAGCCT TCATGCCTGA CAGCTTCCTT 
CGGCCAGGTG GGGACCATGA CTGCGTTCTG GTGCTGTTGC TCATGCCTCG TCTCATTTGC 
AAGGCAGAGC TGATCCGGAA GCAGGCCCAG GAGAAGTTTG AACTAAGTGA GAACTGTTCA 
GAGCGGCCTG GGCTGCGAGG AGCTGCTGGG GAGCAACTCA GCTTTGCTGC TGGACTGGTG 
TACTCGCTGA GCCTGCTGCA GGCCACGCTA CACCGCTATG AGCATGCCCT CTCTCAGTGC 
AGTGTGGATG TGTATAAGAA AGTGGGCAGC CTGTACCCTG AGATGAGTGC CCATGAGCGC 
TCCTTGGATT TCCTCATTGA ACTGCTGCAC AAGGATCAGC TGGATGAGAC TGTCAATGTG 
GAGCCTCTCA CCAAGGCCAT CAAGTACTAT CAGCATCTGT ACAGCATCCA CCTTGCCGAA 
CAGCCTGAGG ACTGTACTAT GCAGCTGGCT GACCACATTA AGTTCACGCA GAGTGCTCTG 
GACTGCATGA GTGTGGAGGT AGGACGGCTG CGTGCCTTCT TGCAGGGTGG GCAGGAGGCT 
ACAGATATTG CCCTCCTGCT CCGGGATCTG GAAACTTCAT GCAGTGACAT CCGCCAGTTC 
TGCAAGAAGA TCCGAAGGCG AATGCCAGGG ACAGATGCTC CTGGGATCCC AGCTGCACTG 
GCCTTTGGAC CACAGGTATC TGACACGCTC CTAGACTGCA GGAAACACTT GACGTGGGTC 
GTGGCTGTGC TGCAGGAGGT GGCAGCTGCT GCTGCCCAGC TCATTGCCCC ACTGGCAGAG 
AATGAGGGGC TACTTGTGGC TGCTCTGGAG GAACTGGCTT TCAAAGCAAG CGAGCAGATC 
TATGGGACCC CCTCCAGCAG CCCCTATGAG TGTCTGCGCC AGTCATGCAA CATCCTCATC 
AGTACCATGA ACAAGCTGGC CACAGCCATG CAGGAGGGGG AGTATGATGC AGAGCGGCCC 
CCCAGCAAGC CTCCACCGGT TGAACTGCGG GCTGCTGCCC TTCGTGCAGA GATCACAGAT 
GCTGAAGGCC TGGGTTTGAA GCTCGAAGAT CGAGAGACAG TTATTAAGGA GTTGAAGAAG 
TCACTCAAGA TTAAGGGAGA GGAGCTAAGT GAGGCCAATG TGCGGCTGAG CCTCCTGGAG 
AAGAAGTTGG ACAGTGCTGC CAAGGATGCA GATGAGCGCA TCGAGAAAGT CCAGACTCGG 
CTGGAGGAGA CCCAGGCACT GCTGCGAAAG AAGGAGAAAG AGTTTGAGGA GACAATGGAT 
GCACTCCAGG CTGACATCGA CCAGCTGGAG GCAGAGAAGG CAGAACTAAA GCAGCGTCTG 
AACAGCCAGT CCAAACGCAC GATTGAGGGA CTCCGGGGCC CTCCTCCTTC AGGCATTGCT 
ACTCTGGTCT CTGGCATTGC TGGTGGAGCC ATCCCTGGGC AGGCTCCAGG GTCTGTGCCA 
GGCCCAGGGC TGGTGAAGGA CTCACCACTG CTGCTTCAGC AGATCTCTGC CATGAGGCTG 
CACATCTCCC AGCTCCAGCA TGAGAACAGC ATCCTCAAGG GAGCCCAGAT GAAGGCATCC 
TTGGCATCCC TGCCCCCTCT GCATGTTGCA AAGCTATCCC ATGAGGGCCC TGGCAGTGAG 
TTACCAGCTG GAGCGCTGTA TCGTAAGACC AGCCAGCTGC TGGAGACATT GAATCAATTG 
AGCACACACA CGCACGTAGT AGACATCACT CGCACCAGCC CTGCTGCCAA GAGCCCGTCG 
GCCCAACTTA TGGAGCAAGT GGCTCAGCTT AAGTCCCTGA GTGACACCGT CGAGAAGCTC 
AAGGATGAGG TCCTCAAGGA GACAGTATCT CAGCGCCCTG GAGCCACAGT ACCCACTGAC 
TTTGCCACCT TCCCTTCATC AGCCTTCCTC AGGGCCAAGG AGGAGCAGCA GGATGACACA 
GTCTACATGG GCAAAGTGAC CTTCTCATGT GCGGCTGGTT TTGGACAGCG ACACCGGCTG 
GTGCTGACCC AGGAGCAGCT GCACCAGCTT CACAGTCGCC TCATCTCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001177765.1
CDS	313..4023
Translation	MSAEASARPLRVGSRVEVIGKGHRGTVAYVGATLFATGKWVGVILDEAKGKNDGTVQGRKYFTCDEGHGIFVRQSQIQVFEDGADTTSPETPDSSASKVLKREGTDTTAKTSKLPTRPASTGVAGASSSLGPSGSASAGELSSSEPSTPAQTPLAAPIIPTPVLTSPGAVPPLPSPSKEEEGLRAQVRDLEEKLETLRLKRAEDKAKLKELEKHKIQLEQVQEWKSKMQEQQADLQRRLKEARKEAKEALEAKERYMEEMADTADAIEMATLDKEMAEERAESLQQEVEALKERVDELTTDLEILKAEIEEKGSDGAASSYQLKQLEEQNARLKDALVRMRDLSSSEKQEHVKLQKLMEKKNQELEVVRQQRERLQEELSQAESTIDELKEQVDAALGAEEMVEMLTDRNLNLEEKVRELRETVGDLEAMNEMNDELQENARETELELREQLDMAGARVREAQKRVEAAQETVADYQQTIKKYRQLTAHLQDVNRELTNQQEASVERQQQPPPETFDFKIKFAETKAHAKAIEMELRQMEVAQANRHMSLLTAFMPDSFLRPGGDHDCVLVLLLMPRLICKAELIRKQAQEKFELSENCSERPGLRGAAGEQLSFAAGLVYSLSLLQATLHRYEHALSQCSVDVYKKVGSLYPEMSAHERSLDFLIELLHKDQLDETVNVEPLTKAIKYYQHLYSIHLAEQPEDCTMQLADHIKFTQSALDCMSVEVGRLRAFLQGGQEATDIALLLRDLETSCSDIRQFCKKIRRRMPGTDAPGIPAALAFGPQVSDTLLDCRKHLTWVVAVLQEVAAAAAQLIAPLAENEGLLVAALEELAFKASEQIYGTPSSSPYECLRQSCNILISTMNKLATAMQEGEYDAERPPSKPPPVELRAAALRAEITDAEGLGLKLEDRETVIKELKKSLKIKGEELSEANVRLSLLEKKLDSAAKDADERIEKVQTRLEETQALLRKKEKEFEETMDALQADIDQLEAEKAELKQRLNSQSKRTIEGLRGPPPSGIATLVSGIAGGAIPGQAPGSVPGPGLVKDSPLLLQQISAMRLHISQLQHENSILKGAQMKASLASLPPLHVAKLSHEGPGSELPAGALYRKTSQLLETLNQLSTHTHVVDITRTSPAAKSPSAQLMEQVAQLKSLSDTVEKLKDEVLKETVSQRPGATVPTDFATFPSSAFLRAKEEQQDDTVYMGKVTFSCAAGFGQRHRLVLTQEQLHQLHSRLIS

Target ORF information:

RefSeq Version	NM_001190836.1
Organism	Homo sapiens(human)
Definition	Homo sapiens dynactin subunit 1 (DCTN1), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001190836.1

ORF Insert Sequence:

ATGAGTGCGG AGGCAAGCGC CCGGCCTCTG CGGGTGGGCT CCCGTGTAGA GGTGATTGGA 
AAAGGCCACC GAGGCACTGT GGCCTATGTT GGAGCCACAC TGTTTGCCAC TGGCAAATGG 
GTAGGCGTGA TTCTGGATGA AGCAAAGGGC AAAAATGATG GAACTGTTCA AGGCAGGAAG 
TACTTCACTT GTGATGAAGG GCATGGCATC TTTGTGCGCC AGTCCCAGAT CCAGGTATTT 
GAAGATGGAG CAGATACTAC TTCCCCAGAG ACACCTGATT CTTCTGCTTC AAAAGTCCTC 
AAAAGAGAGG GAACTGATAC AACTGCAAAG ACTAGCAAAC TGCCCACGCG CCCAGCCAGT 
ACTGGGGTGG CTGGGGCCAG TAGCTCCCTG GGCCCCTCTG GCTCAGCGTC AGCAGGTGAG 
CTGAGCAGCA GTGAGCCCAG CACCCCGGCT CAGACTCCGC TGGCAGCACC CATCATCCCC 
ACGCCGGTCC TCACCTCTCC TGGAGCAGTC CCCCCGCTTC CTTCCCCATC CAAGGAGGAG 
GAGGGACTAA GGGCTCAGGT GCGGGACCTG GAGGAGAAAC TAGAGACCCT GAGACTGAAA 
CGGGCAGAAG ACAAAGCAAA GCTAAAAGAG CTGGAGAAAC ACAAAATCCA GCTGGAGCAG 
GTGCAGGAAT GGAAGAGCAA AATGCAGGAG CAGCAGGCCG ACCTGCAGCG GCGCCTCAAG 
GAGGCGAGAA AGGAAGCCAA GGAGGCGCTG GAGGCAAAGG AACGCTATAT GGAGGAGATG 
GCTGATACTG CTGATGCCAT TGAGATGGCC ACTTTGGACA AGGAGATGGC TGAAGAGCGG 
GCTGAGTCCC TGCAGCAGGA GGTGGAGGCA CTGAAGGAGC GGGTGGACGA GCTCACTACT 
GACTTAGAGA TCCTCAAGGC TGAGATTGAA GAGAAGGGCT CAGATGGCGC TGCATCCAGT 
TATCAGCTCA AGCAGCTTGA GGAGCAGAAT GCCCGCCTGA AGGATGCCCT GGTGAGGATG 
CGGGATCTTT CTTCCTCAGA GAAGCAGGAG CATGTGAAGC TCCAGAAGCT CATGGAAAAG 
AAGAACCAAG AGCTGGAAGT TGTGAGGCAA CAGCGGGAGC GTCTGCAGGA GGAGCTAAGC 
CAGGCAGAGA GCACCATTGA TGAGCTCAAG GAGCAGGTGG ATGCTGCTCT GGGTGCTGAG 
GAGATGGTGG AGATGCTGAC AGATCGGAAC CTGAATCTGG AAGAGAAAGT GCGCGAGTTG 
AGGGAGACTG TGGGAGACTT GGAAGCGATG AATGAGATGA ACGATGAGCT GCAGGAGAAT 
GCACGTGAGA CAGAACTGGA GCTGCGGGAG CAGCTGGACA TGGCAGGCGC GCGGGTTCGT 
GAGGCCCAGA AGCGTGTGGA GGCAGCCCAG GAGACGGTTG CAGACTACCA GCAGACCATC 
AAGAAGTACC GCCAGCTGAC CGCCCATCTA CAGGATGTGA ATCGGGAACT GACAAACCAG 
CAGGAAGCAT CTGTGGAGAG GCAACAGCAG CCACCTCCAG AGACCTTTGA CTTCAAAATC 
AAGTTTGCTG AGACTAAGGC CCATGCCAAG GCAATTGAGA TGGAATTGAG GCAGATGGAG 
GTGGCCCAGG CCAATCGACA CATGTCCCTG CTGACAGCCT TCATGCCTGA CAGCTTCCTT 
CGGCCAGGTG GGGACCATGA CTGCGTTCTG GTGCTGTTGC TCATGCCTCG TCTCATTTGC 
AAGGCAGAGC TGATCCGGAA GCAGGCCCAG GAGAAGTTTG AACTAAGTGA GAACTGTTCA 
GAGCGGCCTG GGCTGCGAGG AGCTGCTGGG GAGCAACTCA GCTTTGCTGC TGGACTGGTG 
TACTCGCTGA GCCTGCTGCA GGCCACGCTA CACCGCTATG AGCATGCCCT CTCTCAGTGC 
AGTGTGGATG TGTATAAGAA AGTGGGCAGC CTGTACCCTG AGATGAGTGC CCATGAGCGC 
TCCTTGGATT TCCTCATTGA ACTGCTGCAC AAGGATCAGC TGGATGAGAC TGTCAATGTG 
GAGCCTCTCA CCAAGGCCAT CAAGTACTAT CAGCATCTGT ACAGCATCCA CCTTGCCGAA 
CAGCCTGAGG ACTGTACTAT GCAGCTGGCT GACCACATTA AGTTCACGCA GAGTGCTCTG 
GACTGCATGA GTGTGGAGGT AGGACGGCTG CGTGCCTTCT TGCAGGGTGG GCAGGAGGCT 
ACAGATATTG CCCTCCTGCT CCGGGATCTG GAAACTTCAT GCAGTGACAT CCGCCAGTTC 
TGCAAGAAGA TCCGAAGGCG AATGCCAGGG ACAGATGCTC CTGGGATCCC AGCTGCACTG 
GCCTTTGGAC CACAGGTATC TGACACGCTC CTAGACTGCA GGAAACACTT GACGTGGGTC 
GTGGCTGTGC TGCAGGAGGT GGCAGCTGCT GCTGCCCAGC TCATTGCCCC ACTGGCAGAG 
AATGAGGGGC TACTTGTGGC TGCTCTGGAG GAACTGGCTT TCAAAGCAAG CGAGCAGATC 
TATGGGACCC CCTCCAGCAG CCCCTATGAG TGTCTGCGCC AGTCATGCAA CATCCTCATC 
AGTACCATGA ACAAGCTGGC CACAGCCATG CAGGAGGGGG AGTATGATGC AGAGCGGCCC 
CCCAGCAAGC CTCCACCGGT TGAACTGCGG GCTGCTGCCC TTCGTGCAGA GATCACAGAT 
GCTGAAGGCC TGGGTTTGAA GCTCGAAGAT CGAGAGACAG TTATTAAGGA GTTGAAGAAG 
TCACTCAAGA TTAAGGGAGA GGAGCTAAGT GAGGCCAATG TGCGGCTGAG CCTCCTGGAG 
AAGAAGTTGG ACAGTGCTGC CAAGGATGCA GATGAGCGCA TCGAGAAAGT CCAGACTCGG 
CTGGAGGAGA CCCAGGCACT GCTGCGAAAG AAGGAGAAAG AGTTTGAGGA GACAATGGAT 
GCACTCCAGG CTGACATCGA CCAGCTGGAG GCAGAGAAGG CAGAACTAAA GCAGCGTCTG 
AACAGCCAGT CCAAACGCAC GATTGAGGGA CTCCGGGGCC CTCCTCCTTC AGGCATTGCT 
ACTCTGGTCT CTGGCATTGC TGGTGGAGCC ATCCCTGGGC AGGCTCCAGG GTCTGTGCCA 
GGCCCAGGGC TGGTGAAGGA CTCACCACTG CTGCTTCAGC AGATCTCTGC CATGAGGCTG 
CACATCTCCC AGCTCCAGCA TGAGAACAGC ATCCTCAAGG GAGCCCAGAT GAAGGCATCC 
TTGGCATCCC TGCCCCCTCT GCATGTTGCA AAGCTATCCC ATGAGGGCCC TGGCAGTGAG 
TTACCAGCTG GAGCGCTGTA TCGTAAGACC AGCCAGCTGC TGGAGACATT GAATCAATTG 
AGCACACACA CGCACGTAGT AGACATCACT CGCACCAGCC CTGCTGCCAA GAGCCCGTCG 
GCCCAACTTA TGGAGCAAGT GGCTCAGCTT AAGTCCCTGA GTGACACCGT CGAGAAGCTC 
AAGGATGAGG TCCTCAAGGA GACAGTATCT CAGCGCCCTG GAGCCACAGT ACCCACTGAC 
TTTGCCACCT TCCCTTCATC AGCCTTCCTC AGGGCCAAGG AGGAGCAGCA GGATGACACA 
GTCTACATGG GCAAAGTGAC CTTCTCATGT GCGGCTGGTT TTGGACAGCG ACACCGGCTG 
GTGCTGACCC AGGAGCAGCT GCACCAGCTT CACAGTCGCC TCATCTCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DCTN1 ( NM_001135041.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001128513.1 Homo sapiens dynactin subunit 1 (DCTN1), transcript variant 4, mRNA.

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CloneID	OHu16697
Clone ID Related Accession (Same CDS sequence)	NM_001135041.2
Accession Version	NM_001135041.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3420bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-06
Organism	Homo sapiens(human)
Product	dynactin subunit 1 isoform 4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA111497.1, BC071583.1, BC013978.2 and CA448857.1. On Jul 1, 2010 this sequence version replaced NM_001135041.1. Transcript Variant: This variant (4) represents the shortest transcript and it encodes the shortest protein (isoform 4). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.209673.1, SRR1803617.265005.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

ATGATGAGAC AGGCACCGAC AGCCCGAAAG ACCACAACTC GGCGACCCAA GCCCACGCGC 
CCAGCCAGTA CTGGGGTGGC TGGGGCCAGT AGCTCCCTGG GCCCCTCTGG CTCAGCGTCA 
GCAGGTGAGC TGAGCAGCAG TGAGCCCAGC ACCCCGGCTC AGACTCCGCT GGCAGCACCC 
ATCATCCCCA CGCCGGTCCT CACCTCTCCT GGAGCAGTCC CCCCGCTTCC TTCCCCATCC 
AAGGAGGAGG AGGGACTAAG GGCTCAGGTG CGGGACCTGG AGGAGAAACT AGAGACCCTG 
AGACTGAAAC GGGCAGAAGA CAAAGCAAAG CTAAAAGAGC TGGAGAAACA CAAAATCCAG 
CTGGAGCAGG TGCAGGAATG GAAGAGCAAA ATGCAGGAGC AGCAGGCCGA CCTGCAGCGG 
CGCCTCAAGG AGGCGAGAAA GGAAGCCAAG GAGGCGCTGG AGGCAAAGGA ACGCTATATG 
GAGGAGATGG CTGATACTGC TGATGCCATT GAGATGGCCA CTTTGGACAA GGAGATGGCT 
GAAGAGCGGG CTGAGTCCCT GCAGCAGGAG GTGGAGGCAC TGAAGGAGCG GGTGGACGAG 
CTCACTACTG ACTTAGAGAT CCTCAAGGCT GAGATTGAAG AGAAGGGCTC AGATGGCGCT 
GCATCCAGTT ATCAGCTCAA GCAGCTTGAG GAGCAGAATG CCCGCCTGAA GGATGCCCTG 
GTGAGGATGC GGGATCTTTC TTCCTCAGAG AAGCAGGAGC ATGTGAAGCT CCAGAAGCTC 
ATGGAAAAGA AGAACCAAGA GCTGGAAGTT GTGAGGCAAC AGCGGGAGCG TCTGCAGGAG 
GAGCTAAGCC AGGCAGAGAG CACCATTGAT GAGCTCAAGG AGCAGGTGGA TGCTGCTCTG 
GGTGCTGAGG AGATGGTGGA GATGCTGACA GATCGGAACC TGAATCTGGA AGAGAAAGTG 
CGCGAGTTGA GGGAGACTGT GGGAGACTTG GAAGCGATGA ATGAGATGAA CGATGAGCTG 
CAGGAGAATG CACGTGAGAC AGAACTGGAG CTGCGGGAGC AGCTGGACAT GGCAGGCGCG 
CGGGTTCGTG AGGCCCAGAA GCGTGTGGAG GCAGCCCAGG AGACGGTTGC AGACTACCAG 
CAGACCATCA AGAAGTACCG CCAGCTGACC GCCCATCTAC AGGATGTGAA TCGGGAACTG 
ACAAACCAGC AGGAAGCATC TGTGGAGAGG CAACAGCAGC CACCTCCAGA GACCTTTGAC 
TTCAAAATCA AGTTTGCTGA GACTAAGGCC CATGCCAAGG CAATTGAGAT GGAATTGAGG 
CAGATGGAGG TGGCCCAGGC CAATCGACAC ATGTCCCTGC TGACAGCCTT CATGCCTGAC 
AGCTTCCTTC GGCCAGGTGG GGACCATGAC TGCGTTCTGG TGCTGTTGCT CATGCCTCGT 
CTCATTTGCA AGGCAGAGCT GATCCGGAAG CAGGCCCAGG AGAAGTTTGA ACTAAGTGAG 
AACTGTTCAG AGCGGCCTGG GCTGCGAGGA GCTGCTGGGG AGCAACTCAG CTTTGCTGCT 
GGACTGGTGT ACTCGCTGAG CCTGCTGCAG GCCACGCTAC ACCGCTATGA GCATGCCCTC 
TCTCAGTGCA GTGTGGATGT GTATAAGAAA GTGGGCAGCC TGTACCCTGA GATGAGTGCC 
CATGAGCGCT CCTTGGATTT CCTCATTGAA CTGCTGCACA AGGATCAGCT GGATGAGACT 
GTCAATGTGG AGCCTCTCAC CAAGGCCATC AAGTACTATC AGCATCTGTA CAGCATCCAC 
CTTGCCGAAC AGCCTGAGGA CTGTACTATG CAGCTGGCTG ACCACATTAA GTTCACGCAG 
AGTGCTCTGG ACTGCATGAG TGTGGAGGTA GGACGGCTGC GTGCCTTCTT GCAGGGTGGG 
CAGGAGGCTA CAGATATTGC CCTCCTGCTC CGGGATCTGG AAACTTCATG CAGTGACATC 
CGCCAGTTCT GCAAGAAGAT CCGAAGGCGA ATGCCAGGGA CAGATGCTCC TGGGATCCCA 
GCTGCACTGG CCTTTGGACC ACAGGTATCT GACACGCTCC TAGACTGCAG GAAACACTTG 
ACGTGGGTCG TGGCTGTGCT GCAGGAGGTG GCAGCTGCTG CTGCCCAGCT CATTGCCCCA 
CTGGCAGAGA ATGAGGGGCT ACTTGTGGCT GCTCTGGAGG AACTGGCTTT CAAAGCAAGC 
GAGCAGATCT ATGGGACCCC CTCCAGCAGC CCCTATGAGT GTCTGCGCCA GTCATGCAAC 
ATCCTCATCA GTACCATGAA CAAGCTGGCC ACAGCCATGC AGGAGGGGGA GTATGATGCA 
GAGCGGCCCC CCAGCAAGCC TCCACCGGTT GAACTGCGGG CTGCTGCCCT TCGTGCAGAG 
ATCACAGATG CTGAAGGCCT GGGTTTGAAG CTCGAAGATC GAGAGACAGT TATTAAGGAG 
TTGAAGAAGT CACTCAAGAT TAAGGGAGAG GAGCTAAGTG AGGCCAATGT GCGGCTGAGC 
CTCCTGGAGA AGAAGTTGGA CAGTGCTGCC AAGGATGCAG ATGAGCGCAT CGAGAAAGTC 
CAGACTCGGC TGGAGGAGAC CCAGGCACTG CTGCGAAAGA AGGAGAAAGA GTTTGAGGAG 
ACAATGGATG CACTCCAGGC TGACATCGAC CAGCTGGAGG CAGAGAAGGC AGAACTAAAG 
CAGCGTCTGA ACAGCCAGTC CAAACGCACG ATTGAGGGAC TCCGGGGCCC TCCTCCTTCA 
GGCATTGCTA CTCTGGTCTC TGGCATTGCT GGTGGAGCCA TCCCTGGGCA GGCTCCAGGG 
TCTGTGCCAG GCCCAGGGCT GGTGAAGGAC TCACCACTGC TGCTTCAGCA GATCTCTGCC 
ATGAGGCTGC ACATCTCCCA GCTCCAGCAT GAGAACAGCA TCCTCAAGGG AGCCCAGATG 
AAGGCATCCT TGGCATCCCT GCCCCCTCTG CATGTTGCAA AGCTATCCCA TGAGGGCCCT 
GGCAGTGAGT TACCAGCTGG AGCGCTGTAT CGTAAGACCA GCCAGCTGCT GGAGACATTG 
AATCAATTGA GCACACACAC GCACGTAGTA GACATCACTC GCACCAGCCC TGCTGCCAAG 
AGCCCGTCGG CCCAACTTAT GGAGCAAGTG GCTCAGCTTA AGTCCCTGAG TGACACCGTC 
GAGAAGCTCA AGGATGAGGT CCTCAAGGAG ACAGTATCTC AGCGCCCTGG AGCCACAGTA 
CCCACTGACT TTGCCACCTT CCCTTCATCA GCCTTCCTCA GGGCCAAGGA GGAGCAGCAG 
GATGACACAG TCTACATGGG CAAAGTGACC TTCTCATGTG CGGCTGGTTT TGGACAGCGA 
CACCGGCTGG TGCTGACCCA GGAGCAGCTG CACCAGCTTC ACAGTCGCCT CATCTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001128513.1
CDS	384..3803
Translation	MMRQAPTARKTTTRRPKPTRPASTGVAGASSSLGPSGSASAGELSSSEPSTPAQTPLAAPIIPTPVLTSPGAVPPLPSPSKEEEGLRAQVRDLEEKLETLRLKRAEDKAKLKELEKHKIQLEQVQEWKSKMQEQQADLQRRLKEARKEAKEALEAKERYMEEMADTADAIEMATLDKEMAEERAESLQQEVEALKERVDELTTDLEILKAEIEEKGSDGAASSYQLKQLEEQNARLKDALVRMRDLSSSEKQEHVKLQKLMEKKNQELEVVRQQRERLQEELSQAESTIDELKEQVDAALGAEEMVEMLTDRNLNLEEKVRELRETVGDLEAMNEMNDELQENARETELELREQLDMAGARVREAQKRVEAAQETVADYQQTIKKYRQLTAHLQDVNRELTNQQEASVERQQQPPPETFDFKIKFAETKAHAKAIEMELRQMEVAQANRHMSLLTAFMPDSFLRPGGDHDCVLVLLLMPRLICKAELIRKQAQEKFELSENCSERPGLRGAAGEQLSFAAGLVYSLSLLQATLHRYEHALSQCSVDVYKKVGSLYPEMSAHERSLDFLIELLHKDQLDETVNVEPLTKAIKYYQHLYSIHLAEQPEDCTMQLADHIKFTQSALDCMSVEVGRLRAFLQGGQEATDIALLLRDLETSCSDIRQFCKKIRRRMPGTDAPGIPAALAFGPQVSDTLLDCRKHLTWVVAVLQEVAAAAAQLIAPLAENEGLLVAALEELAFKASEQIYGTPSSSPYECLRQSCNILISTMNKLATAMQEGEYDAERPPSKPPPVELRAAALRAEITDAEGLGLKLEDRETVIKELKKSLKIKGEELSEANVRLSLLEKKLDSAAKDADERIEKVQTRLEETQALLRKKEKEFEETMDALQADIDQLEAEKAELKQRLNSQSKRTIEGLRGPPPSGIATLVSGIAGGAIPGQAPGSVPGPGLVKDSPLLLQQISAMRLHISQLQHENSILKGAQMKASLASLPPLHVAKLSHEGPGSELPAGALYRKTSQLLETLNQLSTHTHVVDITRTSPAAKSPSAQLMEQVAQLKSLSDTVEKLKDEVLKETVSQRPGATVPTDFATFPSSAFLRAKEEQQDDTVYMGKVTFSCAAGFGQRHRLVLTQEQLHQLHSRLIS

Target ORF information:

RefSeq Version	NM_001135041.2
Organism	Homo sapiens(human)
Definition	Homo sapiens dynactin subunit 1 (DCTN1), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001135041.2

ORF Insert Sequence:

ATGATGAGAC AGGCACCGAC AGCCCGAAAG ACCACAACTC GGCGACCCAA GCCCACGCGC 
CCAGCCAGTA CTGGGGTGGC TGGGGCCAGT AGCTCCCTGG GCCCCTCTGG CTCAGCGTCA 
GCAGGTGAGC TGAGCAGCAG TGAGCCCAGC ACCCCGGCTC AGACTCCGCT GGCAGCACCC 
ATCATCCCCA CGCCGGTCCT CACCTCTCCT GGAGCAGTCC CCCCGCTTCC TTCCCCATCC 
AAGGAGGAGG AGGGACTAAG GGCTCAGGTG CGGGACCTGG AGGAGAAACT AGAGACCCTG 
AGACTGAAAC GGGCAGAAGA CAAAGCAAAG CTAAAAGAGC TGGAGAAACA CAAAATCCAG 
CTGGAGCAGG TGCAGGAATG GAAGAGCAAA ATGCAGGAGC AGCAGGCCGA CCTGCAGCGG 
CGCCTCAAGG AGGCGAGAAA GGAAGCCAAG GAGGCGCTGG AGGCAAAGGA ACGCTATATG 
GAGGAGATGG CTGATACTGC TGATGCCATT GAGATGGCCA CTTTGGACAA GGAGATGGCT 
GAAGAGCGGG CTGAGTCCCT GCAGCAGGAG GTGGAGGCAC TGAAGGAGCG GGTGGACGAG 
CTCACTACTG ACTTAGAGAT CCTCAAGGCT GAGATTGAAG AGAAGGGCTC AGATGGCGCT 
GCATCCAGTT ATCAGCTCAA GCAGCTTGAG GAGCAGAATG CCCGCCTGAA GGATGCCCTG 
GTGAGGATGC GGGATCTTTC TTCCTCAGAG AAGCAGGAGC ATGTGAAGCT CCAGAAGCTC 
ATGGAAAAGA AGAACCAAGA GCTGGAAGTT GTGAGGCAAC AGCGGGAGCG TCTGCAGGAG 
GAGCTAAGCC AGGCAGAGAG CACCATTGAT GAGCTCAAGG AGCAGGTGGA TGCTGCTCTG 
GGTGCTGAGG AGATGGTGGA GATGCTGACA GATCGGAACC TGAATCTGGA AGAGAAAGTG 
CGCGAGTTGA GGGAGACTGT GGGAGACTTG GAAGCGATGA ATGAGATGAA CGATGAGCTG 
CAGGAGAATG CACGTGAGAC AGAACTGGAG CTGCGGGAGC AGCTGGACAT GGCAGGCGCG 
CGGGTTCGTG AGGCCCAGAA GCGTGTGGAG GCAGCCCAGG AGACGGTTGC AGACTACCAG 
CAGACCATCA AGAAGTACCG CCAGCTGACC GCCCATCTAC AGGATGTGAA TCGGGAACTG 
ACAAACCAGC AGGAAGCATC TGTGGAGAGG CAACAGCAGC CACCTCCAGA GACCTTTGAC 
TTCAAAATCA AGTTTGCTGA GACTAAGGCC CATGCCAAGG CAATTGAGAT GGAATTGAGG 
CAGATGGAGG TGGCCCAGGC CAATCGACAC ATGTCCCTGC TGACAGCCTT CATGCCTGAC 
AGCTTCCTTC GGCCAGGTGG GGACCATGAC TGCGTTCTGG TGCTGTTGCT CATGCCTCGT 
CTCATTTGCA AGGCAGAGCT GATCCGGAAG CAGGCCCAGG AGAAGTTTGA ACTAAGTGAG 
AACTGTTCAG AGCGGCCTGG GCTGCGAGGA GCTGCTGGGG AGCAACTCAG CTTTGCTGCT 
GGACTGGTGT ACTCGCTGAG CCTGCTGCAG GCCACGCTAC ACCGCTATGA GCATGCCCTC 
TCTCAGTGCA GTGTGGATGT GTATAAGAAA GTGGGCAGCC TGTACCCTGA GATGAGTGCC 
CATGAGCGCT CCTTGGATTT CCTCATTGAA CTGCTGCACA AGGATCAGCT GGATGAGACT 
GTCAATGTGG AGCCTCTCAC CAAGGCCATC AAGTACTATC AGCATCTGTA CAGCATCCAC 
CTTGCCGAAC AGCCTGAGGA CTGTACTATG CAGCTGGCTG ACCACATTAA GTTCACGCAG 
AGTGCTCTGG ACTGCATGAG TGTGGAGGTA GGACGGCTGC GTGCCTTCTT GCAGGGTGGG 
CAGGAGGCTA CAGATATTGC CCTCCTGCTC CGGGATCTGG AAACTTCATG CAGTGACATC 
CGCCAGTTCT GCAAGAAGAT CCGAAGGCGA ATGCCAGGGA CAGATGCTCC TGGGATCCCA 
GCTGCACTGG CCTTTGGACC ACAGGTATCT GACACGCTCC TAGACTGCAG GAAACACTTG 
ACGTGGGTCG TGGCTGTGCT GCAGGAGGTG GCAGCTGCTG CTGCCCAGCT CATTGCCCCA 
CTGGCAGAGA ATGAGGGGCT ACTTGTGGCT GCTCTGGAGG AACTGGCTTT CAAAGCAAGC 
GAGCAGATCT ATGGGACCCC CTCCAGCAGC CCCTATGAGT GTCTGCGCCA GTCATGCAAC 
ATCCTCATCA GTACCATGAA CAAGCTGGCC ACAGCCATGC AGGAGGGGGA GTATGATGCA 
GAGCGGCCCC CCAGCAAGCC TCCACCGGTT GAACTGCGGG CTGCTGCCCT TCGTGCAGAG 
ATCACAGATG CTGAAGGCCT GGGTTTGAAG CTCGAAGATC GAGAGACAGT TATTAAGGAG 
TTGAAGAAGT CACTCAAGAT TAAGGGAGAG GAGCTAAGTG AGGCCAATGT GCGGCTGAGC 
CTCCTGGAGA AGAAGTTGGA CAGTGCTGCC AAGGATGCAG ATGAGCGCAT CGAGAAAGTC 
CAGACTCGGC TGGAGGAGAC CCAGGCACTG CTGCGAAAGA AGGAGAAAGA GTTTGAGGAG 
ACAATGGATG CACTCCAGGC TGACATCGAC CAGCTGGAGG CAGAGAAGGC AGAACTAAAG 
CAGCGTCTGA ACAGCCAGTC CAAACGCACG ATTGAGGGAC TCCGGGGCCC TCCTCCTTCA 
GGCATTGCTA CTCTGGTCTC TGGCATTGCT GGTGGAGCCA TCCCTGGGCA GGCTCCAGGG 
TCTGTGCCAG GCCCAGGGCT GGTGAAGGAC TCACCACTGC TGCTTCAGCA GATCTCTGCC 
ATGAGGCTGC ACATCTCCCA GCTCCAGCAT GAGAACAGCA TCCTCAAGGG AGCCCAGATG 
AAGGCATCCT TGGCATCCCT GCCCCCTCTG CATGTTGCAA AGCTATCCCA TGAGGGCCCT 
GGCAGTGAGT TACCAGCTGG AGCGCTGTAT CGTAAGACCA GCCAGCTGCT GGAGACATTG 
AATCAATTGA GCACACACAC GCACGTAGTA GACATCACTC GCACCAGCCC TGCTGCCAAG 
AGCCCGTCGG CCCAACTTAT GGAGCAAGTG GCTCAGCTTA AGTCCCTGAG TGACACCGTC 
GAGAAGCTCA AGGATGAGGT CCTCAAGGAG ACAGTATCTC AGCGCCCTGG AGCCACAGTA 
CCCACTGACT TTGCCACCTT CCCTTCATCA GCCTTCCTCA GGGCCAAGGA GGAGCAGCAG 
GATGACACAG TCTACATGGG CAAAGTGACC TTCTCATGTG CGGCTGGTTT TGGACAGCGA 
CACCGGCTGG TGCTGACCCA GGAGCAGCTG CACCAGCTTC ACAGTCGCCT CATCTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DCTN1 ( NM_023019.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_075408.1 Homo sapiens dynactin subunit 1 (DCTN1), transcript variant 2, mRNA.

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CloneID	OHu16728
Clone ID Related Accession (Same CDS sequence)	NM_023019.3
Accession Version	NM_023019.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3435bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-06
Organism	Homo sapiens(human)
Product	dynactin subunit 1 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA111497.1, BC071583.1, BC013978.2, X98801.1 and CA448857.1. This sequence is a reference standard in the RefSeqGene project. On Jul 1, 2010 this sequence version replaced NM_023019.2. Transcript Variant: This variant (2) contains an additional in-frame exon in the 3' end, compared to variant 4, resulting in a protein (isoform 2) with a longer C-terminus, compared to isoform 4. Isoform 2 is also called p135. CCDS Note: This CCDS representation lacks publicly available full-length transcript support. Its exon combination is based on mRNA annotation on DNA accession AF064205.1 (AAD55812.1 product) from PMID:9799602. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.75201.1, SRR1803613.95431.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGATGAGAC AGGCACCGAC AGCCCGAAAG ACCACAACTC GGCGACCCAA GCCCACGCGC 
CCAGCCAGTA CTGGGGTGGC TGGGGCCAGT AGCTCCCTGG GCCCCTCTGG CTCAGCGTCA 
GCAGGTGAGC TGAGCAGCAG TGAGCCCAGC ACCCCGGCTC AGACTCCGCT GGCAGCACCC 
ATCATCCCCA CGCCGGTCCT CACCTCTCCT GGAGCAGTCC CCCCGCTTCC TTCCCCATCC 
AAGGAGGAGG AGGGACTAAG GGCTCAGGTG CGGGACCTGG AGGAGAAACT AGAGACCCTG 
AGACTGAAAC GGGCAGAAGA CAAAGCAAAG CTAAAAGAGC TGGAGAAACA CAAAATCCAG 
CTGGAGCAGG TGCAGGAATG GAAGAGCAAA ATGCAGGAGC AGCAGGCCGA CCTGCAGCGG 
CGCCTCAAGG AGGCGAGAAA GGAAGCCAAG GAGGCGCTGG AGGCAAAGGA ACGCTATATG 
GAGGAGATGG CTGATACTGC TGATGCCATT GAGATGGCCA CTTTGGACAA GGAGATGGCT 
GAAGAGCGGG CTGAGTCCCT GCAGCAGGAG GTGGAGGCAC TGAAGGAGCG GGTGGACGAG 
CTCACTACTG ACTTAGAGAT CCTCAAGGCT GAGATTGAAG AGAAGGGCTC AGATGGCGCT 
GCATCCAGTT ATCAGCTCAA GCAGCTTGAG GAGCAGAATG CCCGCCTGAA GGATGCCCTG 
GTGAGGATGC GGGATCTTTC TTCCTCAGAG AAGCAGGAGC ATGTGAAGCT CCAGAAGCTC 
ATGGAAAAGA AGAACCAAGA GCTGGAAGTT GTGAGGCAAC AGCGGGAGCG TCTGCAGGAG 
GAGCTAAGCC AGGCAGAGAG CACCATTGAT GAGCTCAAGG AGCAGGTGGA TGCTGCTCTG 
GGTGCTGAGG AGATGGTGGA GATGCTGACA GATCGGAACC TGAATCTGGA AGAGAAAGTG 
CGCGAGTTGA GGGAGACTGT GGGAGACTTG GAAGCGATGA ATGAGATGAA CGATGAGCTG 
CAGGAGAATG CACGTGAGAC AGAACTGGAG CTGCGGGAGC AGCTGGACAT GGCAGGCGCG 
CGGGTTCGTG AGGCCCAGAA GCGTGTGGAG GCAGCCCAGG AGACGGTTGC AGACTACCAG 
CAGACCATCA AGAAGTACCG CCAGCTGACC GCCCATCTAC AGGATGTGAA TCGGGAACTG 
ACAAACCAGC AGGAAGCATC TGTGGAGAGG CAACAGCAGC CACCTCCAGA GACCTTTGAC 
TTCAAAATCA AGTTTGCTGA GACTAAGGCC CATGCCAAGG CAATTGAGAT GGAATTGAGG 
CAGATGGAGG TGGCCCAGGC CAATCGACAC ATGTCCCTGC TGACAGCCTT CATGCCTGAC 
AGCTTCCTTC GGCCAGGTGG GGACCATGAC TGCGTTCTGG TGCTGTTGCT CATGCCTCGT 
CTCATTTGCA AGGCAGAGCT GATCCGGAAG CAGGCCCAGG AGAAGTTTGA ACTAAGTGAG 
AACTGTTCAG AGCGGCCTGG GCTGCGAGGA GCTGCTGGGG AGCAACTCAG CTTTGCTGCT 
GGACTGGTGT ACTCGCTGAG CCTGCTGCAG GCCACGCTAC ACCGCTATGA GCATGCCCTC 
TCTCAGTGCA GTGTGGATGT GTATAAGAAA GTGGGCAGCC TGTACCCTGA GATGAGTGCC 
CATGAGCGCT CCTTGGATTT CCTCATTGAA CTGCTGCACA AGGATCAGCT GGATGAGACT 
GTCAATGTGG AGCCTCTCAC CAAGGCCATC AAGTACTATC AGCATCTGTA CAGCATCCAC 
CTTGCCGAAC AGCCTGAGGA CTGTACTATG CAGCTGGCTG ACCACATTAA GTTCACGCAG 
AGTGCTCTGG ACTGCATGAG TGTGGAGGTA GGACGGCTGC GTGCCTTCTT GCAGGGTGGG 
CAGGAGGCTA CAGATATTGC CCTCCTGCTC CGGGATCTGG AAACTTCATG CAGTGACATC 
CGCCAGTTCT GCAAGAAGAT CCGAAGGCGA ATGCCAGGGA CAGATGCTCC TGGGATCCCA 
GCTGCACTGG CCTTTGGACC ACAGGTATCT GACACGCTCC TAGACTGCAG GAAACACTTG 
ACGTGGGTCG TGGCTGTGCT GCAGGAGGTG GCAGCTGCTG CTGCCCAGCT CATTGCCCCA 
CTGGCAGAGA ATGAGGGGCT ACTTGTGGCT GCTCTGGAGG AACTGGCTTT CAAAGCAAGC 
GAGCAGATCT ATGGGACCCC CTCCAGCAGC CCCTATGAGT GTCTGCGCCA GTCATGCAAC 
ATCCTCATCA GTACCATGAA CAAGCTGGCC ACAGCCATGC AGGAGGGGGA GTATGATGCA 
GAGCGGCCCC CCAGCAAGCC TCCACCGGTT GAACTGCGGG CTGCTGCCCT TCGTGCAGAG 
ATCACAGATG CTGAAGGCCT GGGTTTGAAG CTCGAAGATC GAGAGACAGT TATTAAGGAG 
TTGAAGAAGT CACTCAAGAT TAAGGGAGAG GAGCTAAGTG AGGCCAATGT GCGGCTGAGC 
CTCCTGGAGA AGAAGTTGGA CAGTGCTGCC AAGGATGCAG ATGAGCGCAT CGAGAAAGTC 
CAGACTCGGC TGGAGGAGAC CCAGGCACTG CTGCGAAAGA AGGAGAAAGA GTTTGAGGAG 
ACAATGGATG CACTCCAGGC TGACATCGAC CAGCTGGAGG CAGAGAAGGC AGAACTAAAG 
CAGCGTCTGA ACAGCCAGTC CAAACGCACG ATTGAGGGAC TCCGGGGCCC TCCTCCTTCA 
GGCATTGCTA CTCTGGTCTC TGGCATTGCT GGTGAAGAAC AGCAGCGAGG AGCCATCCCT 
GGGCAGGCTC CAGGGTCTGT GCCAGGCCCA GGGCTGGTGA AGGACTCACC ACTGCTGCTT 
CAGCAGATCT CTGCCATGAG GCTGCACATC TCCCAGCTCC AGCATGAGAA CAGCATCCTC 
AAGGGAGCCC AGATGAAGGC ATCCTTGGCA TCCCTGCCCC CTCTGCATGT TGCAAAGCTA 
TCCCATGAGG GCCCTGGCAG TGAGTTACCA GCTGGAGCGC TGTATCGTAA GACCAGCCAG 
CTGCTGGAGA CATTGAATCA ATTGAGCACA CACACGCACG TAGTAGACAT CACTCGCACC 
AGCCCTGCTG CCAAGAGCCC GTCGGCCCAA CTTATGGAGC AAGTGGCTCA GCTTAAGTCC 
CTGAGTGACA CCGTCGAGAA GCTCAAGGAT GAGGTCCTCA AGGAGACAGT ATCTCAGCGC 
CCTGGAGCCA CAGTACCCAC TGACTTTGCC ACCTTCCCTT CATCAGCCTT CCTCAGGGCC 
AAGGAGGAGC AGCAGGATGA CACAGTCTAC ATGGGCAAAG TGACCTTCTC ATGTGCGGCT 
GGTTTTGGAC AGCGACACCG GCTGGTGCTG ACCCAGGAGC AGCTGCACCA GCTTCACAGT 
CGCCTCATCT CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_075408.1
CDS	384..3818
Translation	MMRQAPTARKTTTRRPKPTRPASTGVAGASSSLGPSGSASAGELSSSEPSTPAQTPLAAPIIPTPVLTSPGAVPPLPSPSKEEEGLRAQVRDLEEKLETLRLKRAEDKAKLKELEKHKIQLEQVQEWKSKMQEQQADLQRRLKEARKEAKEALEAKERYMEEMADTADAIEMATLDKEMAEERAESLQQEVEALKERVDELTTDLEILKAEIEEKGSDGAASSYQLKQLEEQNARLKDALVRMRDLSSSEKQEHVKLQKLMEKKNQELEVVRQQRERLQEELSQAESTIDELKEQVDAALGAEEMVEMLTDRNLNLEEKVRELRETVGDLEAMNEMNDELQENARETELELREQLDMAGARVREAQKRVEAAQETVADYQQTIKKYRQLTAHLQDVNRELTNQQEASVERQQQPPPETFDFKIKFAETKAHAKAIEMELRQMEVAQANRHMSLLTAFMPDSFLRPGGDHDCVLVLLLMPRLICKAELIRKQAQEKFELSENCSERPGLRGAAGEQLSFAAGLVYSLSLLQATLHRYEHALSQCSVDVYKKVGSLYPEMSAHERSLDFLIELLHKDQLDETVNVEPLTKAIKYYQHLYSIHLAEQPEDCTMQLADHIKFTQSALDCMSVEVGRLRAFLQGGQEATDIALLLRDLETSCSDIRQFCKKIRRRMPGTDAPGIPAALAFGPQVSDTLLDCRKHLTWVVAVLQEVAAAAAQLIAPLAENEGLLVAALEELAFKASEQIYGTPSSSPYECLRQSCNILISTMNKLATAMQEGEYDAERPPSKPPPVELRAAALRAEITDAEGLGLKLEDRETVIKELKKSLKIKGEELSEANVRLSLLEKKLDSAAKDADERIEKVQTRLEETQALLRKKEKEFEETMDALQADIDQLEAEKAELKQRLNSQSKRTIEGLRGPPPSGIATLVSGIAGEEQQRGAIPGQAPGSVPGPGLVKDSPLLLQQISAMRLHISQLQHENSILKGAQMKASLASLPPLHVAKLSHEGPGSELPAGALYRKTSQLLETLNQLSTHTHVVDITRTSPAAKSPSAQLMEQVAQLKSLSDTVEKLKDEVLKETVSQRPGATVPTDFATFPSSAFLRAKEEQQDDTVYMGKVTFSCAAGFGQRHRLVLTQEQLHQLHSRLIS

Target ORF information:

RefSeq Version	NM_023019.3
Organism	Homo sapiens(human)
Definition	Homo sapiens dynactin subunit 1 (DCTN1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_023019.3

ORF Insert Sequence:

ATGATGAGAC AGGCACCGAC AGCCCGAAAG ACCACAACTC GGCGACCCAA GCCCACGCGC 
CCAGCCAGTA CTGGGGTGGC TGGGGCCAGT AGCTCCCTGG GCCCCTCTGG CTCAGCGTCA 
GCAGGTGAGC TGAGCAGCAG TGAGCCCAGC ACCCCGGCTC AGACTCCGCT GGCAGCACCC 
ATCATCCCCA CGCCGGTCCT CACCTCTCCT GGAGCAGTCC CCCCGCTTCC TTCCCCATCC 
AAGGAGGAGG AGGGACTAAG GGCTCAGGTG CGGGACCTGG AGGAGAAACT AGAGACCCTG 
AGACTGAAAC GGGCAGAAGA CAAAGCAAAG CTAAAAGAGC TGGAGAAACA CAAAATCCAG 
CTGGAGCAGG TGCAGGAATG GAAGAGCAAA ATGCAGGAGC AGCAGGCCGA CCTGCAGCGG 
CGCCTCAAGG AGGCGAGAAA GGAAGCCAAG GAGGCGCTGG AGGCAAAGGA ACGCTATATG 
GAGGAGATGG CTGATACTGC TGATGCCATT GAGATGGCCA CTTTGGACAA GGAGATGGCT 
GAAGAGCGGG CTGAGTCCCT GCAGCAGGAG GTGGAGGCAC TGAAGGAGCG GGTGGACGAG 
CTCACTACTG ACTTAGAGAT CCTCAAGGCT GAGATTGAAG AGAAGGGCTC AGATGGCGCT 
GCATCCAGTT ATCAGCTCAA GCAGCTTGAG GAGCAGAATG CCCGCCTGAA GGATGCCCTG 
GTGAGGATGC GGGATCTTTC TTCCTCAGAG AAGCAGGAGC ATGTGAAGCT CCAGAAGCTC 
ATGGAAAAGA AGAACCAAGA GCTGGAAGTT GTGAGGCAAC AGCGGGAGCG TCTGCAGGAG 
GAGCTAAGCC AGGCAGAGAG CACCATTGAT GAGCTCAAGG AGCAGGTGGA TGCTGCTCTG 
GGTGCTGAGG AGATGGTGGA GATGCTGACA GATCGGAACC TGAATCTGGA AGAGAAAGTG 
CGCGAGTTGA GGGAGACTGT GGGAGACTTG GAAGCGATGA ATGAGATGAA CGATGAGCTG 
CAGGAGAATG CACGTGAGAC AGAACTGGAG CTGCGGGAGC AGCTGGACAT GGCAGGCGCG 
CGGGTTCGTG AGGCCCAGAA GCGTGTGGAG GCAGCCCAGG AGACGGTTGC AGACTACCAG 
CAGACCATCA AGAAGTACCG CCAGCTGACC GCCCATCTAC AGGATGTGAA TCGGGAACTG 
ACAAACCAGC AGGAAGCATC TGTGGAGAGG CAACAGCAGC CACCTCCAGA GACCTTTGAC 
TTCAAAATCA AGTTTGCTGA GACTAAGGCC CATGCCAAGG CAATTGAGAT GGAATTGAGG 
CAGATGGAGG TGGCCCAGGC CAATCGACAC ATGTCCCTGC TGACAGCCTT CATGCCTGAC 
AGCTTCCTTC GGCCAGGTGG GGACCATGAC TGCGTTCTGG TGCTGTTGCT CATGCCTCGT 
CTCATTTGCA AGGCAGAGCT GATCCGGAAG CAGGCCCAGG AGAAGTTTGA ACTAAGTGAG 
AACTGTTCAG AGCGGCCTGG GCTGCGAGGA GCTGCTGGGG AGCAACTCAG CTTTGCTGCT 
GGACTGGTGT ACTCGCTGAG CCTGCTGCAG GCCACGCTAC ACCGCTATGA GCATGCCCTC 
TCTCAGTGCA GTGTGGATGT GTATAAGAAA GTGGGCAGCC TGTACCCTGA GATGAGTGCC 
CATGAGCGCT CCTTGGATTT CCTCATTGAA CTGCTGCACA AGGATCAGCT GGATGAGACT 
GTCAATGTGG AGCCTCTCAC CAAGGCCATC AAGTACTATC AGCATCTGTA CAGCATCCAC 
CTTGCCGAAC AGCCTGAGGA CTGTACTATG CAGCTGGCTG ACCACATTAA GTTCACGCAG 
AGTGCTCTGG ACTGCATGAG TGTGGAGGTA GGACGGCTGC GTGCCTTCTT GCAGGGTGGG 
CAGGAGGCTA CAGATATTGC CCTCCTGCTC CGGGATCTGG AAACTTCATG CAGTGACATC 
CGCCAGTTCT GCAAGAAGAT CCGAAGGCGA ATGCCAGGGA CAGATGCTCC TGGGATCCCA 
GCTGCACTGG CCTTTGGACC ACAGGTATCT GACACGCTCC TAGACTGCAG GAAACACTTG 
ACGTGGGTCG TGGCTGTGCT GCAGGAGGTG GCAGCTGCTG CTGCCCAGCT CATTGCCCCA 
CTGGCAGAGA ATGAGGGGCT ACTTGTGGCT GCTCTGGAGG AACTGGCTTT CAAAGCAAGC 
GAGCAGATCT ATGGGACCCC CTCCAGCAGC CCCTATGAGT GTCTGCGCCA GTCATGCAAC 
ATCCTCATCA GTACCATGAA CAAGCTGGCC ACAGCCATGC AGGAGGGGGA GTATGATGCA 
GAGCGGCCCC CCAGCAAGCC TCCACCGGTT GAACTGCGGG CTGCTGCCCT TCGTGCAGAG 
ATCACAGATG CTGAAGGCCT GGGTTTGAAG CTCGAAGATC GAGAGACAGT TATTAAGGAG 
TTGAAGAAGT CACTCAAGAT TAAGGGAGAG GAGCTAAGTG AGGCCAATGT GCGGCTGAGC 
CTCCTGGAGA AGAAGTTGGA CAGTGCTGCC AAGGATGCAG ATGAGCGCAT CGAGAAAGTC 
CAGACTCGGC TGGAGGAGAC CCAGGCACTG CTGCGAAAGA AGGAGAAAGA GTTTGAGGAG 
ACAATGGATG CACTCCAGGC TGACATCGAC CAGCTGGAGG CAGAGAAGGC AGAACTAAAG 
CAGCGTCTGA ACAGCCAGTC CAAACGCACG ATTGAGGGAC TCCGGGGCCC TCCTCCTTCA 
GGCATTGCTA CTCTGGTCTC TGGCATTGCT GGTGAAGAAC AGCAGCGAGG AGCCATCCCT 
GGGCAGGCTC CAGGGTCTGT GCCAGGCCCA GGGCTGGTGA AGGACTCACC ACTGCTGCTT 
CAGCAGATCT CTGCCATGAG GCTGCACATC TCCCAGCTCC AGCATGAGAA CAGCATCCTC 
AAGGGAGCCC AGATGAAGGC ATCCTTGGCA TCCCTGCCCC CTCTGCATGT TGCAAAGCTA 
TCCCATGAGG GCCCTGGCAG TGAGTTACCA GCTGGAGCGC TGTATCGTAA GACCAGCCAG 
CTGCTGGAGA CATTGAATCA ATTGAGCACA CACACGCACG TAGTAGACAT CACTCGCACC 
AGCCCTGCTG CCAAGAGCCC GTCGGCCCAA CTTATGGAGC AAGTGGCTCA GCTTAAGTCC 
CTGAGTGACA CCGTCGAGAA GCTCAAGGAT GAGGTCCTCA AGGAGACAGT ATCTCAGCGC 
CCTGGAGCCA CAGTACCCAC TGACTTTGCC ACCTTCCCTT CATCAGCCTT CCTCAGGGCC 
AAGGAGGAGC AGCAGGATGA CACAGTCTAC ATGGGCAAAG TGACCTTCTC ATGTGCGGCT 
GGTTTTGGAC AGCGACACCG GCTGGTGCTG ACCCAGGAGC AGCTGCACCA GCTTCACAGT 
CGCCTCATCT CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DCTN1 ( NM_001135040.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001128512.1 Homo sapiens dynactin subunit 1 (DCTN1), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu16796
Clone ID Related Accession (Same CDS sequence)	NM_001135040.2
Accession Version	NM_001135040.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3762bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Homo sapiens(human)
Product	dynactin subunit 1 isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA315227.1, AK314352.1, BC013978.2 and CA448857.1. On Jul 1, 2010 this sequence version replaced NM_001135040.1. Transcript Variant: This variant (3) has multiple differences in the 5' coding region and 5' UTR, compared to variant 4. These differences cause translation initiation from an upstream AUG and a protein (isoform 3) with a longer N-terminus containing a CAP-Gly domain, compared to isoform 4. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.122950.1, SRR1803614.15255.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

ATGGCACAGA GCAAGAGGCA CGTGTACAGC CGGACGCCCA GCGGCAGCAG GATGAGTGCG 
GAGGCAAGCG CCCGGCCTCT GCGGGTGGGC TCCCGTGTAG AGGTGATTGG AAAAGGCCAC 
CGAGGCACTG TGGCCTATGT TGGAGCCACA CTGTTTGCCA CTGGCAAATG GGTAGGCGTG 
ATTCTGGATG AAGCAAAGGG CAAAAATGAT GGAACTGTTC AAGGCAGGAA GTACTTCACT 
TGTGATGAAG GGCATGGCAT CTTTGTGCGC CAGTCCCAGA TCCAGGTATT TGAAGATGGA 
GCAGATACTA CTTCCCCAGA GACACCTGAT TCTTCTGCTT CAAAAGTCCT CAAAAGAGAG 
GGAACTGATA CAACTGCAAA GACTAGCAAA CTGCCCACGC GCCCAGCCAG TACTGGGGTG 
GCTGGGGCCA GTAGCTCCCT GGGCCCCTCT GGCTCAGCGT CAGCAGGTGA GCTGAGCAGC 
AGTGAGCCCA GCACCCCGGC TCAGACTCCG CTGGCAGCAC CCATCATCCC CACGCCGGTC 
CTCACCTCTC CTGGAGCAGT CCCCCCGCTT CCTTCCCCAT CCAAGGAGGA GGAGGGACTA 
AGGGCTCAGG TGCGGGACCT GGAGGAGAAA CTAGAGACCC TGAGACTGAA ACGGGCAGAA 
GACAAAGCAA AGCTAAAAGA GCTGGAGAAA CACAAAATCC AGCTGGAGCA GGTGCAGGAA 
TGGAAGAGCA AAATGCAGGA GCAGCAGGCC GACCTGCAGC GGCGCCTCAA GGAGGCGAGA 
AAGGAAGCCA AGGAGGCGCT GGAGGCAAAG GAACGCTATA TGGAGGAGAT GGCTGATACT 
GCTGATGCCA TTGAGATGGC CACTTTGGAC AAGGAGATGG CTGAAGAGCG GGCTGAGTCC 
CTGCAGCAGG AGGTGGAGGC ACTGAAGGAG CGGGTGGACG AGCTCACTAC TGACTTAGAG 
ATCCTCAAGG CTGAGATTGA AGAGAAGGGC TCAGATGGCG CTGCATCCAG TTATCAGCTC 
AAGCAGCTTG AGGAGCAGAA TGCCCGCCTG AAGGATGCCC TGGTGAGGAT GCGGGATCTT 
TCTTCCTCAG AGAAGCAGGA GCATGTGAAG CTCCAGAAGC TCATGGAAAA GAAGAACCAA 
GAGCTGGAAG TTGTGAGGCA ACAGCGGGAG CGTCTGCAGG AGGAGCTAAG CCAGGCAGAG 
AGCACCATTG ATGAGCTCAA GGAGCAGGTG GATGCTGCTC TGGGTGCTGA GGAGATGGTG 
GAGATGCTGA CAGATCGGAA CCTGAATCTG GAAGAGAAAG TGCGCGAGTT GAGGGAGACT 
GTGGGAGACT TGGAAGCGAT GAATGAGATG AACGATGAGC TGCAGGAGAA TGCACGTGAG 
ACAGAACTGG AGCTGCGGGA GCAGCTGGAC ATGGCAGGCG CGCGGGTTCG TGAGGCCCAG 
AAGCGTGTGG AGGCAGCCCA GGAGACGGTT GCAGACTACC AGCAGACCAT CAAGAAGTAC 
CGCCAGCTGA CCGCCCATCT ACAGGATGTG AATCGGGAAC TGACAAACCA GCAGGAAGCA 
TCTGTGGAGA GGCAACAGCA GCCACCTCCA GAGACCTTTG ACTTCAAAAT CAAGTTTGCT 
GAGACTAAGG CCCATGCCAA GGCAATTGAG ATGGAATTGA GGCAGATGGA GGTGGCCCAG 
GCCAATCGAC ACATGTCCCT GCTGACAGCC TTCATGCCTG ACAGCTTCCT TCGGCCAGGT 
GGGGACCATG ACTGCGTTCT GGTGCTGTTG CTCATGCCTC GTCTCATTTG CAAGGCAGAG 
CTGATCCGGA AGCAGGCCCA GGAGAAGTTT GAACTAAGTG AGAACTGTTC AGAGCGGCCT 
GGGCTGCGAG GAGCTGCTGG GGAGCAACTC AGCTTTGCTG CTGGACTGGT GTACTCGCTG 
AGCCTGCTGC AGGCCACGCT ACACCGCTAT GAGCATGCCC TCTCTCAGTG CAGTGTGGAT 
GTGTATAAGA AAGTGGGCAG CCTGTACCCT GAGATGAGTG CCCATGAGCG CTCCTTGGAT 
TTCCTCATTG AACTGCTGCA CAAGGATCAG CTGGATGAGA CTGTCAATGT GGAGCCTCTC 
ACCAAGGCCA TCAAGTACTA TCAGCATCTG TACAGCATCC ACCTTGCCGA ACAGCCTGAG 
GACTGTACTA TGCAGCTGGC TGACCACATT AAGTTCACGC AGAGTGCTCT GGACTGCATG 
AGTGTGGAGG TAGGACGGCT GCGTGCCTTC TTGCAGGGTG GGCAGGAGGC TACAGATATT 
GCCCTCCTGC TCCGGGATCT GGAAACTTCA TGCAGTGACA TCCGCCAGTT CTGCAAGAAG 
ATCCGAAGGC GAATGCCAGG GACAGATGCT CCTGGGATCC CAGCTGCACT GGCCTTTGGA 
CCACAGGTAT CTGACACGCT CCTAGACTGC AGGAAACACT TGACGTGGGT CGTGGCTGTG 
CTGCAGGAGG TGGCAGCTGC TGCTGCCCAG CTCATTGCCC CACTGGCAGA GAATGAGGGG 
CTACTTGTGG CTGCTCTGGA GGAACTGGCT TTCAAAGCAA GCGAGCAGAT CTATGGGACC 
CCCTCCAGCA GCCCCTATGA GTGTCTGCGC CAGTCATGCA ACATCCTCAT CAGTACCATG 
AACAAGCTGG CCACAGCCAT GCAGGAGGGG GAGTATGATG CAGAGCGGCC CCCCAGCAAG 
CCTCCACCGG TTGAACTGCG GGCTGCTGCC CTTCGTGCAG AGATCACAGA TGCTGAAGGC 
CTGGGTTTGA AGCTCGAAGA TCGAGAGACA GTTATTAAGG AGTTGAAGAA GTCACTCAAG 
ATTAAGGGAG AGGAGCTAAG TGAGGCCAAT GTGCGGCTGA GCCTCCTGGA GAAGAAGTTG 
GACAGTGCTG CCAAGGATGC AGATGAGCGC ATCGAGAAAG TCCAGACTCG GCTGGAGGAG 
ACCCAGGCAC TGCTGCGAAA GAAGGAGAAA GAGTTTGAGG AGACAATGGA TGCACTCCAG 
GCTGACATCG ACCAGCTGGA GGCAGAGAAG GCAGAACTAA AGCAGCGTCT GAACAGCCAG 
TCCAAACGCA CGATTGAGGG ACTCCGGGGC CCTCCTCCTT CAGGCATTGC TACTCTGGTC 
TCTGGCATTG CTGGTGGAGC CATCCCTGGG CAGGCTCCAG GGTCTGTGCC AGGCCCAGGG 
CTGGTGAAGG ACTCACCACT GCTGCTTCAG CAGATCTCTG CCATGAGGCT GCACATCTCC 
CAGCTCCAGC ATGAGAACAG CATCCTCAAG GGAGCCCAGA TGAAGGCATC CTTGGCATCC 
CTGCCCCCTC TGCATGTTGC AAAGCTATCC CATGAGGGCC CTGGCAGTGA GTTACCAGCT 
GGAGCGCTGT ATCGTAAGAC CAGCCAGCTG CTGGAGACAT TGAATCAATT GAGCACACAC 
ACGCACGTAG TAGACATCAC TCGCACCAGC CCTGCTGCCA AGAGCCCGTC GGCCCAACTT 
ATGGAGCAAG TGGCTCAGCT TAAGTCCCTG AGTGACACCG TCGAGAAGCT CAAGGATGAG 
GTCCTCAAGG AGACAGTATC TCAGCGCCCT GGAGCCACAG TACCCACTGA CTTTGCCACC 
TTCCCTTCAT CAGCCTTCCT CAGGGCCAAG GAGGAGCAGC AGGATGACAC AGTCTACATG 
GGCAAAGTGA CCTTCTCATG TGCGGCTGGT TTTGGACAGC GACACCGGCT GGTGCTGACC 
CAGGAGCAGC TGCACCAGCT TCACAGTCGC CTCATCTCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001128512.1
CDS	319..4080
Translation	MAQSKRHVYSRTPSGSRMSAEASARPLRVGSRVEVIGKGHRGTVAYVGATLFATGKWVGVILDEAKGKNDGTVQGRKYFTCDEGHGIFVRQSQIQVFEDGADTTSPETPDSSASKVLKREGTDTTAKTSKLPTRPASTGVAGASSSLGPSGSASAGELSSSEPSTPAQTPLAAPIIPTPVLTSPGAVPPLPSPSKEEEGLRAQVRDLEEKLETLRLKRAEDKAKLKELEKHKIQLEQVQEWKSKMQEQQADLQRRLKEARKEAKEALEAKERYMEEMADTADAIEMATLDKEMAEERAESLQQEVEALKERVDELTTDLEILKAEIEEKGSDGAASSYQLKQLEEQNARLKDALVRMRDLSSSEKQEHVKLQKLMEKKNQELEVVRQQRERLQEELSQAESTIDELKEQVDAALGAEEMVEMLTDRNLNLEEKVRELRETVGDLEAMNEMNDELQENARETELELREQLDMAGARVREAQKRVEAAQETVADYQQTIKKYRQLTAHLQDVNRELTNQQEASVERQQQPPPETFDFKIKFAETKAHAKAIEMELRQMEVAQANRHMSLLTAFMPDSFLRPGGDHDCVLVLLLMPRLICKAELIRKQAQEKFELSENCSERPGLRGAAGEQLSFAAGLVYSLSLLQATLHRYEHALSQCSVDVYKKVGSLYPEMSAHERSLDFLIELLHKDQLDETVNVEPLTKAIKYYQHLYSIHLAEQPEDCTMQLADHIKFTQSALDCMSVEVGRLRAFLQGGQEATDIALLLRDLETSCSDIRQFCKKIRRRMPGTDAPGIPAALAFGPQVSDTLLDCRKHLTWVVAVLQEVAAAAAQLIAPLAENEGLLVAALEELAFKASEQIYGTPSSSPYECLRQSCNILISTMNKLATAMQEGEYDAERPPSKPPPVELRAAALRAEITDAEGLGLKLEDRETVIKELKKSLKIKGEELSEANVRLSLLEKKLDSAAKDADERIEKVQTRLEETQALLRKKEKEFEETMDALQADIDQLEAEKAELKQRLNSQSKRTIEGLRGPPPSGIATLVSGIAGGAIPGQAPGSVPGPGLVKDSPLLLQQISAMRLHISQLQHENSILKGAQMKASLASLPPLHVAKLSHEGPGSELPAGALYRKTSQLLETLNQLSTHTHVVDITRTSPAAKSPSAQLMEQVAQLKSLSDTVEKLKDEVLKETVSQRPGATVPTDFATFPSSAFLRAKEEQQDDTVYMGKVTFSCAAGFGQRHRLVLTQEQLHQLHSRLIS

Target ORF information:

RefSeq Version	NM_001135040.2
Organism	Homo sapiens(human)
Definition	Homo sapiens dynactin subunit 1 (DCTN1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001135040.2

ORF Insert Sequence:

ATGGCACAGA GCAAGAGGCA CGTGTACAGC CGGACGCCCA GCGGCAGCAG GATGAGTGCG 
GAGGCAAGCG CCCGGCCTCT GCGGGTGGGC TCCCGTGTAG AGGTGATTGG AAAAGGCCAC 
CGAGGCACTG TGGCCTATGT TGGAGCCACA CTGTTTGCCA CTGGCAAATG GGTAGGCGTG 
ATTCTGGATG AAGCAAAGGG CAAAAATGAT GGAACTGTTC AAGGCAGGAA GTACTTCACT 
TGTGATGAAG GGCATGGCAT CTTTGTGCGC CAGTCCCAGA TCCAGGTATT TGAAGATGGA 
GCAGATACTA CTTCCCCAGA GACACCTGAT TCTTCTGCTT CAAAAGTCCT CAAAAGAGAG 
GGAACTGATA CAACTGCAAA GACTAGCAAA CTGCCCACGC GCCCAGCCAG TACTGGGGTG 
GCTGGGGCCA GTAGCTCCCT GGGCCCCTCT GGCTCAGCGT CAGCAGGTGA GCTGAGCAGC 
AGTGAGCCCA GCACCCCGGC TCAGACTCCG CTGGCAGCAC CCATCATCCC CACGCCGGTC 
CTCACCTCTC CTGGAGCAGT CCCCCCGCTT CCTTCCCCAT CCAAGGAGGA GGAGGGACTA 
AGGGCTCAGG TGCGGGACCT GGAGGAGAAA CTAGAGACCC TGAGACTGAA ACGGGCAGAA 
GACAAAGCAA AGCTAAAAGA GCTGGAGAAA CACAAAATCC AGCTGGAGCA GGTGCAGGAA 
TGGAAGAGCA AAATGCAGGA GCAGCAGGCC GACCTGCAGC GGCGCCTCAA GGAGGCGAGA 
AAGGAAGCCA AGGAGGCGCT GGAGGCAAAG GAACGCTATA TGGAGGAGAT GGCTGATACT 
GCTGATGCCA TTGAGATGGC CACTTTGGAC AAGGAGATGG CTGAAGAGCG GGCTGAGTCC 
CTGCAGCAGG AGGTGGAGGC ACTGAAGGAG CGGGTGGACG AGCTCACTAC TGACTTAGAG 
ATCCTCAAGG CTGAGATTGA AGAGAAGGGC TCAGATGGCG CTGCATCCAG TTATCAGCTC 
AAGCAGCTTG AGGAGCAGAA TGCCCGCCTG AAGGATGCCC TGGTGAGGAT GCGGGATCTT 
TCTTCCTCAG AGAAGCAGGA GCATGTGAAG CTCCAGAAGC TCATGGAAAA GAAGAACCAA 
GAGCTGGAAG TTGTGAGGCA ACAGCGGGAG CGTCTGCAGG AGGAGCTAAG CCAGGCAGAG 
AGCACCATTG ATGAGCTCAA GGAGCAGGTG GATGCTGCTC TGGGTGCTGA GGAGATGGTG 
GAGATGCTGA CAGATCGGAA CCTGAATCTG GAAGAGAAAG TGCGCGAGTT GAGGGAGACT 
GTGGGAGACT TGGAAGCGAT GAATGAGATG AACGATGAGC TGCAGGAGAA TGCACGTGAG 
ACAGAACTGG AGCTGCGGGA GCAGCTGGAC ATGGCAGGCG CGCGGGTTCG TGAGGCCCAG 
AAGCGTGTGG AGGCAGCCCA GGAGACGGTT GCAGACTACC AGCAGACCAT CAAGAAGTAC 
CGCCAGCTGA CCGCCCATCT ACAGGATGTG AATCGGGAAC TGACAAACCA GCAGGAAGCA 
TCTGTGGAGA GGCAACAGCA GCCACCTCCA GAGACCTTTG ACTTCAAAAT CAAGTTTGCT 
GAGACTAAGG CCCATGCCAA GGCAATTGAG ATGGAATTGA GGCAGATGGA GGTGGCCCAG 
GCCAATCGAC ACATGTCCCT GCTGACAGCC TTCATGCCTG ACAGCTTCCT TCGGCCAGGT 
GGGGACCATG ACTGCGTTCT GGTGCTGTTG CTCATGCCTC GTCTCATTTG CAAGGCAGAG 
CTGATCCGGA AGCAGGCCCA GGAGAAGTTT GAACTAAGTG AGAACTGTTC AGAGCGGCCT 
GGGCTGCGAG GAGCTGCTGG GGAGCAACTC AGCTTTGCTG CTGGACTGGT GTACTCGCTG 
AGCCTGCTGC AGGCCACGCT ACACCGCTAT GAGCATGCCC TCTCTCAGTG CAGTGTGGAT 
GTGTATAAGA AAGTGGGCAG CCTGTACCCT GAGATGAGTG CCCATGAGCG CTCCTTGGAT 
TTCCTCATTG AACTGCTGCA CAAGGATCAG CTGGATGAGA CTGTCAATGT GGAGCCTCTC 
ACCAAGGCCA TCAAGTACTA TCAGCATCTG TACAGCATCC ACCTTGCCGA ACAGCCTGAG 
GACTGTACTA TGCAGCTGGC TGACCACATT AAGTTCACGC AGAGTGCTCT GGACTGCATG 
AGTGTGGAGG TAGGACGGCT GCGTGCCTTC TTGCAGGGTG GGCAGGAGGC TACAGATATT 
GCCCTCCTGC TCCGGGATCT GGAAACTTCA TGCAGTGACA TCCGCCAGTT CTGCAAGAAG 
ATCCGAAGGC GAATGCCAGG GACAGATGCT CCTGGGATCC CAGCTGCACT GGCCTTTGGA 
CCACAGGTAT CTGACACGCT CCTAGACTGC AGGAAACACT TGACGTGGGT CGTGGCTGTG 
CTGCAGGAGG TGGCAGCTGC TGCTGCCCAG CTCATTGCCC CACTGGCAGA GAATGAGGGG 
CTACTTGTGG CTGCTCTGGA GGAACTGGCT TTCAAAGCAA GCGAGCAGAT CTATGGGACC 
CCCTCCAGCA GCCCCTATGA GTGTCTGCGC CAGTCATGCA ACATCCTCAT CAGTACCATG 
AACAAGCTGG CCACAGCCAT GCAGGAGGGG GAGTATGATG CAGAGCGGCC CCCCAGCAAG 
CCTCCACCGG TTGAACTGCG GGCTGCTGCC CTTCGTGCAG AGATCACAGA TGCTGAAGGC 
CTGGGTTTGA AGCTCGAAGA TCGAGAGACA GTTATTAAGG AGTTGAAGAA GTCACTCAAG 
ATTAAGGGAG AGGAGCTAAG TGAGGCCAAT GTGCGGCTGA GCCTCCTGGA GAAGAAGTTG 
GACAGTGCTG CCAAGGATGC AGATGAGCGC ATCGAGAAAG TCCAGACTCG GCTGGAGGAG 
ACCCAGGCAC TGCTGCGAAA GAAGGAGAAA GAGTTTGAGG AGACAATGGA TGCACTCCAG 
GCTGACATCG ACCAGCTGGA GGCAGAGAAG GCAGAACTAA AGCAGCGTCT GAACAGCCAG 
TCCAAACGCA CGATTGAGGG ACTCCGGGGC CCTCCTCCTT CAGGCATTGC TACTCTGGTC 
TCTGGCATTG CTGGTGGAGC CATCCCTGGG CAGGCTCCAG GGTCTGTGCC AGGCCCAGGG 
CTGGTGAAGG ACTCACCACT GCTGCTTCAG CAGATCTCTG CCATGAGGCT GCACATCTCC 
CAGCTCCAGC ATGAGAACAG CATCCTCAAG GGAGCCCAGA TGAAGGCATC CTTGGCATCC 
CTGCCCCCTC TGCATGTTGC AAAGCTATCC CATGAGGGCC CTGGCAGTGA GTTACCAGCT 
GGAGCGCTGT ATCGTAAGAC CAGCCAGCTG CTGGAGACAT TGAATCAATT GAGCACACAC 
ACGCACGTAG TAGACATCAC TCGCACCAGC CCTGCTGCCA AGAGCCCGTC GGCCCAACTT 
ATGGAGCAAG TGGCTCAGCT TAAGTCCCTG AGTGACACCG TCGAGAAGCT CAAGGATGAG 
GTCCTCAAGG AGACAGTATC TCAGCGCCCT GGAGCCACAG TACCCACTGA CTTTGCCACC 
TTCCCTTCAT CAGCCTTCCT CAGGGCCAAG GAGGAGCAGC AGGATGACAC AGTCTACATG 
GGCAAAGTGA CCTTCTCATG TGCGGCTGGT TTTGGACAGC GACACCGGCT GGTGCTGACC 
CAGGAGCAGC TGCACCAGCT TCACAGTCGC CTCATCTCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DCTN1 ( NM_001190837.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001177766.1 Homo sapiens dynactin subunit 1 (DCTN1), transcript variant 6, mRNA.

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CloneID	OHu16952
Clone ID Related Accession (Same CDS sequence)	NM_001190837.1
Accession Version	NM_001190837.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3816bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Homo sapiens(human)
Product	dynactin subunit 1 isoform 6
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA315227.1, AK314352.1, X98801.1, BX640799.1 and CA448857.1. Transcript Variant: This variant (6) has multiple differences in the 5' end and in the 3' coding region, compared to variant 4. These differences cause translation initiation from an upstream AUG and a protein (isoform 6) with longer N- and C-termini, compared to isoform 4. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

ATGGCACAGA GCAAGAGGCA CGTGTACAGC CGGACGCCCA GCGGCAGCAG GATGAGTGCG 
GAGGCAAGCG CCCGGCCTCT GCGGGTGGGC TCCCGTGTAG AGGTGATTGG AAAAGGCCAC 
CGAGGCACTG TGGCCTATGT TGGAGCCACA CTGTTTGCCA CTGGCAAATG GGTAGGCGTG 
ATTCTGGATG AAGCAAAGGG CAAAAATGAT GGAACTGTTC AAGGCAGGAA GTACTTCACT 
TGTGATGAAG GGCATGGCAT CTTTGTGCGC CAGTCCCAGA TCCAGGTATT TGAAGATGGA 
GCAGATACTA CTTCCCCAGA GACACCTGAT TCTTCTGCTT CAAAAGTCCT CAAAAGAGAG 
GGAACTGATA CAACTGCAAA GACTAGCAAA CTGGCACCGA CAGCCCGAAA GACCACAACT 
CGGCGACCCA AGCCCACGCG CCCAGCCAGT ACTGGGGTGG CTGGGGCCAG TAGCTCCCTG 
GGCCCCTCTG GCTCAGCGTC AGCAGGTGAG CTGAGCAGCA GTGAGCCCAG CACCCCGGCT 
CAGACTCCGC TGGCAGCACC CATCATCCCC ACGCCGGTCC TCACCTCTCC TGGAGCAGTC 
CCCCCGCTTC CTTCCCCATC CAAGGAGGAG GAGGGACTAA GGGCTCAGGT GCGGGACCTG 
GAGGAGAAAC TAGAGACCCT GAGACTGAAA CGGGCAGAAG ACAAAGCAAA GCTAAAAGAG 
CTGGAGAAAC ACAAAATCCA GCTGGAGCAG GTGCAGGAAT GGAAGAGCAA AATGCAGGAG 
CAGCAGGCCG ACCTGCAGCG GCGCCTCAAG GAGGCGAGAA AGGAAGCCAA GGAGGCGCTG 
GAGGCAAAGG AACGCTATAT GGAGGAGATG GCTGATACTG CTGATGCCAT TGAGATGGCC 
ACTTTGGACA AGGAGATGGC TGAAGAGCGG GCTGAGTCCC TGCAGCAGGA GGTGGAGGCA 
CTGAAGGAGC GGGTGGACGA GCTCACTACT GACTTAGAGA TCCTCAAGGC TGAGATTGAA 
GAGAAGGGCT CAGATGGCGC TGCATCCAGT TATCAGCTCA AGCAGCTTGA GGAGCAGAAT 
GCCCGCCTGA AGGATGCCCT GGTGAGGATG CGGGATCTTT CTTCCTCAGA GAAGCAGGAG 
CATGTGAAGC TCCAGAAGCT CATGGAAAAG AAGAACCAAG AGCTGGAAGT TGTGAGGCAA 
CAGCGGGAGC GTCTGCAGGA GGAGCTAAGC CAGGCAGAGA GCACCATTGA TGAGCTCAAG 
GAGCAGGTGG ATGCTGCTCT GGGTGCTGAG GAGATGGTGG AGATGCTGAC AGATCGGAAC 
CTGAATCTGG AAGAGAAAGT GCGCGAGTTG AGGGAGACTG TGGGAGACTT GGAAGCGATG 
AATGAGATGA ACGATGAGCT GCAGGAGAAT GCACGTGAGA CAGAACTGGA GCTGCGGGAG 
CAGCTGGACA TGGCAGGCGC GCGGGTTCGT GAGGCCCAGA AGCGTGTGGA GGCAGCCCAG 
GAGACGGTTG CAGACTACCA GCAGACCATC AAGAAGTACC GCCAGCTGAC CGCCCATCTA 
CAGGATGTGA ATCGGGAACT GACAAACCAG CAGGAAGCAT CTGTGGAGAG GCAACAGCAG 
CCACCTCCAG AGACCTTTGA CTTCAAAATC AAGTTTGCTG AGACTAAGGC CCATGCCAAG 
GCAATTGAGA TGGAATTGAG GCAGATGGAG GTGGCCCAGG CCAATCGACA CATGTCCCTG 
CTGACAGCCT TCATGCCTGA CAGCTTCCTT CGGCCAGGTG GGGACCATGA CTGCGTTCTG 
GTGCTGTTGC TCATGCCTCG TCTCATTTGC AAGGCAGAGC TGATCCGGAA GCAGGCCCAG 
GAGAAGTTTG AACTAAGTGA GAACTGTTCA GAGCGGCCTG GGCTGCGAGG AGCTGCTGGG 
GAGCAACTCA GCTTTGCTGC TGGACTGGTG TACTCGCTGA GCCTGCTGCA GGCCACGCTA 
CACCGCTATG AGCATGCCCT CTCTCAGTGC AGTGTGGATG TGTATAAGAA AGTGGGCAGC 
CTGTACCCTG AGATGAGTGC CCATGAGCGC TCCTTGGATT TCCTCATTGA ACTGCTGCAC 
AAGGATCAGC TGGATGAGAC TGTCAATGTG GAGCCTCTCA CCAAGGCCAT CAAGTACTAT 
CAGCATCTGT ACAGCATCCA CCTTGCCGAA CAGCCTGAGG ACTGTACTAT GCAGCTGGCT 
GACCACATTA AGTTCACGCA GAGTGCTCTG GACTGCATGA GTGTGGAGGT AGGACGGCTG 
CGTGCCTTCT TGCAGGGTGG GCAGGAGGCT ACAGATATTG CCCTCCTGCT CCGGGATCTG 
GAAACTTCAT GCAGTGACAT CCGCCAGTTC TGCAAGAAGA TCCGAAGGCG AATGCCAGGG 
ACAGATGCTC CTGGGATCCC AGCTGCACTG GCCTTTGGAC CACAGGTATC TGACACGCTC 
CTAGACTGCA GGAAACACTT GACGTGGGTC GTGGCTGTGC TGCAGGAGGT GGCAGCTGCT 
GCTGCCCAGC TCATTGCCCC ACTGGCAGAG AATGAGGGGC TACTTGTGGC TGCTCTGGAG 
GAACTGGCTT TCAAAGCAAG CGAGCAGATC TATGGGACCC CCTCCAGCAG CCCCTATGAG 
TGTCTGCGCC AGTCATGCAA CATCCTCATC AGTACCATGA ACAAGCTGGC CACAGCCATG 
CAGGAGGGGG AGTATGATGC AGAGCGGCCC CCCAGCAAGC CTCCACCGGT TGAACTGCGG 
GCTGCTGCCC TTCGTGCAGA GATCACAGAT GCTGAAGGCC TGGGTTTGAA GCTCGAAGAT 
CGAGAGACAG TTATTAAGGA GTTGAAGAAG TCACTCAAGA TTAAGGGAGA GGAGCTAAGT 
GAGGCCAATG TGCGGCTGAG CCTCCTGGAG AAGAAGTTGG ACAGTGCTGC CAAGGATGCA 
GATGAGCGCA TCGAGAAAGT CCAGACTCGG CTGGAGGAGA CCCAGGCACT GCTGCGAAAG 
AAGGAGAAAG AGTTTGAGGA GACAATGGAT GCACTCCAGG CTGACATCGA CCAGCTGGAG 
GCAGAGAAGG CAGAACTAAA GCAGCGTCTG AACAGCCAGT CCAAACGCAC GATTGAGGGA 
CTCCGGGGCC CTCCTCCTTC AGGCATTGCT ACTCTGGTCT CTGGCATTGC TGGTGAAGAA 
CAGCAGCGAG GAGCCATCCC TGGGCAGGCT CCAGGGTCTG TGCCAGGCCC AGGGCTGGTG 
AAGGACTCAC CACTGCTGCT TCAGCAGATC TCTGCCATGA GGCTGCACAT CTCCCAGCTC 
CAGCATGAGA ACAGCATCCT CAAGGGAGCC CAGATGAAGG CATCCTTGGC ATCCCTGCCC 
CCTCTGCATG TTGCAAAGCT ATCCCATGAG GGCCCTGGCA GTGAGTTACC AGCTGGAGCG 
CTGTATCGTA AGACCAGCCA GCTGCTGGAG ACATTGAATC AATTGAGCAC ACACACGCAC 
GTAGTAGACA TCACTCGCAC CAGCCCTGCT GCCAAGAGCC CGTCGGCCCA ACTTATGGAG 
CAAGTGGCTC AGCTTAAGTC CCTGAGTGAC ACCGTCGAGA AGCTCAAGGA TGAGGTCCTC 
AAGGAGACAG TATCTCAGCG CCCTGGAGCC ACAGTACCCA CTGACTTTGC CACCTTCCCT 
TCATCAGCCT TCCTCAGGGC CAAGGAGGAG CAGCAGGATG ACACAGTCTA CATGGGCAAA 
GTGACCTTCT CATGTGCGGC TGGTTTTGGA CAGCGACACC GGCTGGTGCT GACCCAGGAG 
CAGCTGCACC AGCTTCACAG TCGCCTCATC TCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001177766.1
CDS	319..4134
Translation	MAQSKRHVYSRTPSGSRMSAEASARPLRVGSRVEVIGKGHRGTVAYVGATLFATGKWVGVILDEAKGKNDGTVQGRKYFTCDEGHGIFVRQSQIQVFEDGADTTSPETPDSSASKVLKREGTDTTAKTSKLAPTARKTTTRRPKPTRPASTGVAGASSSLGPSGSASAGELSSSEPSTPAQTPLAAPIIPTPVLTSPGAVPPLPSPSKEEEGLRAQVRDLEEKLETLRLKRAEDKAKLKELEKHKIQLEQVQEWKSKMQEQQADLQRRLKEARKEAKEALEAKERYMEEMADTADAIEMATLDKEMAEERAESLQQEVEALKERVDELTTDLEILKAEIEEKGSDGAASSYQLKQLEEQNARLKDALVRMRDLSSSEKQEHVKLQKLMEKKNQELEVVRQQRERLQEELSQAESTIDELKEQVDAALGAEEMVEMLTDRNLNLEEKVRELRETVGDLEAMNEMNDELQENARETELELREQLDMAGARVREAQKRVEAAQETVADYQQTIKKYRQLTAHLQDVNRELTNQQEASVERQQQPPPETFDFKIKFAETKAHAKAIEMELRQMEVAQANRHMSLLTAFMPDSFLRPGGDHDCVLVLLLMPRLICKAELIRKQAQEKFELSENCSERPGLRGAAGEQLSFAAGLVYSLSLLQATLHRYEHALSQCSVDVYKKVGSLYPEMSAHERSLDFLIELLHKDQLDETVNVEPLTKAIKYYQHLYSIHLAEQPEDCTMQLADHIKFTQSALDCMSVEVGRLRAFLQGGQEATDIALLLRDLETSCSDIRQFCKKIRRRMPGTDAPGIPAALAFGPQVSDTLLDCRKHLTWVVAVLQEVAAAAAQLIAPLAENEGLLVAALEELAFKASEQIYGTPSSSPYECLRQSCNILISTMNKLATAMQEGEYDAERPPSKPPPVELRAAALRAEITDAEGLGLKLEDRETVIKELKKSLKIKGEELSEANVRLSLLEKKLDSAAKDADERIEKVQTRLEETQALLRKKEKEFEETMDALQADIDQLEAEKAELKQRLNSQSKRTIEGLRGPPPSGIATLVSGIAGEEQQRGAIPGQAPGSVPGPGLVKDSPLLLQQISAMRLHISQLQHENSILKGAQMKASLASLPPLHVAKLSHEGPGSELPAGALYRKTSQLLETLNQLSTHTHVVDITRTSPAAKSPSAQLMEQVAQLKSLSDTVEKLKDEVLKETVSQRPGATVPTDFATFPSSAFLRAKEEQQDDTVYMGKVTFSCAAGFGQRHRLVLTQEQLHQLHSRLIS

Target ORF information:

RefSeq Version	NM_001190837.1
Organism	Homo sapiens(human)
Definition	Homo sapiens dynactin subunit 1 (DCTN1), transcript variant 6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001190837.1

ORF Insert Sequence:

ATGGCACAGA GCAAGAGGCA CGTGTACAGC CGGACGCCCA GCGGCAGCAG GATGAGTGCG 
GAGGCAAGCG CCCGGCCTCT GCGGGTGGGC TCCCGTGTAG AGGTGATTGG AAAAGGCCAC 
CGAGGCACTG TGGCCTATGT TGGAGCCACA CTGTTTGCCA CTGGCAAATG GGTAGGCGTG 
ATTCTGGATG AAGCAAAGGG CAAAAATGAT GGAACTGTTC AAGGCAGGAA GTACTTCACT 
TGTGATGAAG GGCATGGCAT CTTTGTGCGC CAGTCCCAGA TCCAGGTATT TGAAGATGGA 
GCAGATACTA CTTCCCCAGA GACACCTGAT TCTTCTGCTT CAAAAGTCCT CAAAAGAGAG 
GGAACTGATA CAACTGCAAA GACTAGCAAA CTGGCACCGA CAGCCCGAAA GACCACAACT 
CGGCGACCCA AGCCCACGCG CCCAGCCAGT ACTGGGGTGG CTGGGGCCAG TAGCTCCCTG 
GGCCCCTCTG GCTCAGCGTC AGCAGGTGAG CTGAGCAGCA GTGAGCCCAG CACCCCGGCT 
CAGACTCCGC TGGCAGCACC CATCATCCCC ACGCCGGTCC TCACCTCTCC TGGAGCAGTC 
CCCCCGCTTC CTTCCCCATC CAAGGAGGAG GAGGGACTAA GGGCTCAGGT GCGGGACCTG 
GAGGAGAAAC TAGAGACCCT GAGACTGAAA CGGGCAGAAG ACAAAGCAAA GCTAAAAGAG 
CTGGAGAAAC ACAAAATCCA GCTGGAGCAG GTGCAGGAAT GGAAGAGCAA AATGCAGGAG 
CAGCAGGCCG ACCTGCAGCG GCGCCTCAAG GAGGCGAGAA AGGAAGCCAA GGAGGCGCTG 
GAGGCAAAGG AACGCTATAT GGAGGAGATG GCTGATACTG CTGATGCCAT TGAGATGGCC 
ACTTTGGACA AGGAGATGGC TGAAGAGCGG GCTGAGTCCC TGCAGCAGGA GGTGGAGGCA 
CTGAAGGAGC GGGTGGACGA GCTCACTACT GACTTAGAGA TCCTCAAGGC TGAGATTGAA 
GAGAAGGGCT CAGATGGCGC TGCATCCAGT TATCAGCTCA AGCAGCTTGA GGAGCAGAAT 
GCCCGCCTGA AGGATGCCCT GGTGAGGATG CGGGATCTTT CTTCCTCAGA GAAGCAGGAG 
CATGTGAAGC TCCAGAAGCT CATGGAAAAG AAGAACCAAG AGCTGGAAGT TGTGAGGCAA 
CAGCGGGAGC GTCTGCAGGA GGAGCTAAGC CAGGCAGAGA GCACCATTGA TGAGCTCAAG 
GAGCAGGTGG ATGCTGCTCT GGGTGCTGAG GAGATGGTGG AGATGCTGAC AGATCGGAAC 
CTGAATCTGG AAGAGAAAGT GCGCGAGTTG AGGGAGACTG TGGGAGACTT GGAAGCGATG 
AATGAGATGA ACGATGAGCT GCAGGAGAAT GCACGTGAGA CAGAACTGGA GCTGCGGGAG 
CAGCTGGACA TGGCAGGCGC GCGGGTTCGT GAGGCCCAGA AGCGTGTGGA GGCAGCCCAG 
GAGACGGTTG CAGACTACCA GCAGACCATC AAGAAGTACC GCCAGCTGAC CGCCCATCTA 
CAGGATGTGA ATCGGGAACT GACAAACCAG CAGGAAGCAT CTGTGGAGAG GCAACAGCAG 
CCACCTCCAG AGACCTTTGA CTTCAAAATC AAGTTTGCTG AGACTAAGGC CCATGCCAAG 
GCAATTGAGA TGGAATTGAG GCAGATGGAG GTGGCCCAGG CCAATCGACA CATGTCCCTG 
CTGACAGCCT TCATGCCTGA CAGCTTCCTT CGGCCAGGTG GGGACCATGA CTGCGTTCTG 
GTGCTGTTGC TCATGCCTCG TCTCATTTGC AAGGCAGAGC TGATCCGGAA GCAGGCCCAG 
GAGAAGTTTG AACTAAGTGA GAACTGTTCA GAGCGGCCTG GGCTGCGAGG AGCTGCTGGG 
GAGCAACTCA GCTTTGCTGC TGGACTGGTG TACTCGCTGA GCCTGCTGCA GGCCACGCTA 
CACCGCTATG AGCATGCCCT CTCTCAGTGC AGTGTGGATG TGTATAAGAA AGTGGGCAGC 
CTGTACCCTG AGATGAGTGC CCATGAGCGC TCCTTGGATT TCCTCATTGA ACTGCTGCAC 
AAGGATCAGC TGGATGAGAC TGTCAATGTG GAGCCTCTCA CCAAGGCCAT CAAGTACTAT 
CAGCATCTGT ACAGCATCCA CCTTGCCGAA CAGCCTGAGG ACTGTACTAT GCAGCTGGCT 
GACCACATTA AGTTCACGCA GAGTGCTCTG GACTGCATGA GTGTGGAGGT AGGACGGCTG 
CGTGCCTTCT TGCAGGGTGG GCAGGAGGCT ACAGATATTG CCCTCCTGCT CCGGGATCTG 
GAAACTTCAT GCAGTGACAT CCGCCAGTTC TGCAAGAAGA TCCGAAGGCG AATGCCAGGG 
ACAGATGCTC CTGGGATCCC AGCTGCACTG GCCTTTGGAC CACAGGTATC TGACACGCTC 
CTAGACTGCA GGAAACACTT GACGTGGGTC GTGGCTGTGC TGCAGGAGGT GGCAGCTGCT 
GCTGCCCAGC TCATTGCCCC ACTGGCAGAG AATGAGGGGC TACTTGTGGC TGCTCTGGAG 
GAACTGGCTT TCAAAGCAAG CGAGCAGATC TATGGGACCC CCTCCAGCAG CCCCTATGAG 
TGTCTGCGCC AGTCATGCAA CATCCTCATC AGTACCATGA ACAAGCTGGC CACAGCCATG 
CAGGAGGGGG AGTATGATGC AGAGCGGCCC CCCAGCAAGC CTCCACCGGT TGAACTGCGG 
GCTGCTGCCC TTCGTGCAGA GATCACAGAT GCTGAAGGCC TGGGTTTGAA GCTCGAAGAT 
CGAGAGACAG TTATTAAGGA GTTGAAGAAG TCACTCAAGA TTAAGGGAGA GGAGCTAAGT 
GAGGCCAATG TGCGGCTGAG CCTCCTGGAG AAGAAGTTGG ACAGTGCTGC CAAGGATGCA 
GATGAGCGCA TCGAGAAAGT CCAGACTCGG CTGGAGGAGA CCCAGGCACT GCTGCGAAAG 
AAGGAGAAAG AGTTTGAGGA GACAATGGAT GCACTCCAGG CTGACATCGA CCAGCTGGAG 
GCAGAGAAGG CAGAACTAAA GCAGCGTCTG AACAGCCAGT CCAAACGCAC GATTGAGGGA 
CTCCGGGGCC CTCCTCCTTC AGGCATTGCT ACTCTGGTCT CTGGCATTGC TGGTGAAGAA 
CAGCAGCGAG GAGCCATCCC TGGGCAGGCT CCAGGGTCTG TGCCAGGCCC AGGGCTGGTG 
AAGGACTCAC CACTGCTGCT TCAGCAGATC TCTGCCATGA GGCTGCACAT CTCCCAGCTC 
CAGCATGAGA ACAGCATCCT CAAGGGAGCC CAGATGAAGG CATCCTTGGC ATCCCTGCCC 
CCTCTGCATG TTGCAAAGCT ATCCCATGAG GGCCCTGGCA GTGAGTTACC AGCTGGAGCG 
CTGTATCGTA AGACCAGCCA GCTGCTGGAG ACATTGAATC AATTGAGCAC ACACACGCAC 
GTAGTAGACA TCACTCGCAC CAGCCCTGCT GCCAAGAGCC CGTCGGCCCA ACTTATGGAG 
CAAGTGGCTC AGCTTAAGTC CCTGAGTGAC ACCGTCGAGA AGCTCAAGGA TGAGGTCCTC 
AAGGAGACAG TATCTCAGCG CCCTGGAGCC ACAGTACCCA CTGACTTTGC CACCTTCCCT 
TCATCAGCCT TCCTCAGGGC CAAGGAGGAG CAGCAGGATG ACACAGTCTA CATGGGCAAA 
GTGACCTTCT CATGTGCGGC TGGTTTTGGA CAGCGACACC GGCTGGTGCT GACCCAGGAG 
CAGCTGCACC AGCTTCACAG TCGCCTCATC TCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Characterization of functional domains of human EB1 family proteins.
The Journal of biological chemistry278(50)49721-31(2003 Dec)
Bu W,Su LK

Evidence that an interaction between EB1 and p150(Glued) is required for the formation and maintenance of a radial microtubule array anchored at the centrosome.
Molecular biology of the cell13(10)3627-45(2002 Oct)
Askham JM,Vaughan KT,Goodson HV,Morrison EE

Genomic organization of the DCTN1-SLC4A5 locus encoding both NBC4 and p150(Glued).
Cytogenetics and cell genetics95(3-4)163-8(2001)
Pushkin A,Abuladze N,Newman D,Tatishchev S,Kurtz I

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DCTN1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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