SPRED1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	SPRED1
Entrez Gene ID	161742
Full Name	sprouty related EVH1 domain containing 1
Synonyms	NFLS,PPP1R147,hSpred1,spred-1
General protein information	Preferred Names sprouty related EVH1 domain containing 1 Names sprouty-related, EVH1 domain-containing protein 1 protein phosphatase 1, regulatory subunit 147 suppressor of Ras/MAPK activation
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 15 Map Location: 15q14
Summary	The protein encoded by this gene is a member of the Sprouty family of proteins and is phosphorylated by tyrosine kinase in response to several growth factors. The encoded protein can act as a homodimer or as a heterodimer with SPRED2 to regulate activation of the MAP kinase cascade. Defects in this gene are a cause of neurofibromatosis type 1-like syndrome (NFLS). [provided by RefSeq, Jul 2008].
Disorder MIM:	609291
Disorder Html:	Legius syndrome, 611431 (3)

mRNA and Protein(s)

mRNA	Protein	Name
XM_011521289.2	XP_011519591.1	sprouty-related, EVH1 domain-containing protein 1 isoform X2
NM_152594.3	NP_689807.1	sprouty-related, EVH1 domain-containing protein 1
XM_005254202.3	XP_005254259.1	sprouty-related, EVH1 domain-containing protein 1 isoform X1
NM_152594.2	NP_689807.1	sprouty-related, EVH1 domain-containing protein 1
XM_011521289.3	XP_011519591.1	sprouty-related, EVH1 domain-containing protein 1 isoform X2

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	SPRED1	XP_544609.3
Rattus norvegicus (Norway rat)	Spred1	NP_001040554.1
Xenopus tropicalis (tropical clawed frog)	spred1	NP_001008037.1
Gallus gallus (chicken)	SPRED1	NP_001186638.1
Pan troglodytes (chimpanzee)	SPRED1	XP_001138171.1
Bos taurus (cattle)	SPRED1	NP_001179445.1
Mus musculus (house mouse)	Spred1	NP_277059.1
Danio rerio (zebrafish)	spred1	NP_998397.1
Homo sapiens (human)	SPRED1	NP_689807.1

Gene Ontology
Bibliography

Related articles in PubMed

The first Slovak Legius syndrome patient carrying the SPRED1 gene mutation.
Sekelska M, Briatkova L, Olcak T, Bolcekova A, Ilencikova D, Kadasi L, Zatkova A
General physiology and biophysics36(2)205-210(2017 Apr)

Potential diagnostic implications of miR-144 overexpression in human oesophageal cancer.
Sharma P, Saraya A, Sharma R
The Indian journal of medical research143(Supplement)S91-S103(2016 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

In one case we identified a nonsense mutation c.46C>T (p.Arg16*) in exon 2 of SPRED1 gene, confirming diagnosis of Legius syndrome. This mutation was reported previously.
Title: The first Slovak Legius syndrome patient carrying the SPRED1 gene mutation.

Results provide genetic evidence that miR-126, through its target gene Spred-1, plays a critical role in the development of retinal vascular layers.
Title: Strand and Cell Type-specific Function of microRNA-126 in Angiogenesis.

The EVH1 domain of Spred1 binds to the noncatalytic portion of the GAP-related domain of neurofibromin.
Title: The neurofibromin recruitment factor Spred1 binds to the GAP related domain without affecting Ras inactivation.

PURA may be a potential target of miR-144 and observed downregulation of PURA may be caused by increased expression of miR-144. The other predicted target of miR-144 SPRED1, was found to be downregulated in 69 per cent EC tissues as compared to matched distant non-malignant tissues.
Title: Potential diagnostic implications of miR-144 overexpression in human oesophageal cancer.

Data suggest SPRED1 EVH1 domain interacts with NF1 GRD domain [N-term. 16AA/C-term. 20AA of GTPase-activating protein-related domain]; SPRED1 EVH1 and NF1 GRD mutations observed in Legius syndrome reduce binding affinity between EVH1/GRD domains.
Title: Interaction between a Domain of the Negative Regulator of the Ras-ERK Pathway, SPRED1 Protein, and the GTPase-activating Protein-related Domain of Neurofibromin Is Implicated in Legius Syndrome and Neurofibromatosis Type 1.

The following SPRED1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SPRED1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu18529	NM_152594.3 Latest version!	Homo sapiens sprouty related EVH1 domain containing 1 (SPRED1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu48143	XM_005254202.3 Latest version!	Homo sapiens sprouty related EVH1 domain containing 1 (SPRED1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OHu77141	XM_011521289.3 Latest version!	Homo sapiens sprouty related EVH1 domain containing 1 (SPRED1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
View Old Accession Versions >>
OHu18529	NM_152594.2	Homo sapiens sprouty related EVH1 domain containing 1 (SPRED1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu77141	XM_011521289.2	Homo sapiens sprouty related EVH1 domain containing 1 (SPRED1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SPRED1 ( NM_152594.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_689807.1 Homo sapiens sprouty related EVH1 domain containing 1 (SPRED1), mRNA.

Price & Availability↑

CloneID	OHu18529
Clone ID Related Accession (Same CDS sequence)	NM_152594.3 , NM_152594.2
Accession Version	NM_152594.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1335bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-21
Organism	Homo sapiens(human)
Product	sprouty-related, EVH1 domain-containing protein 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC069381.8, DA405650.1 and AY299089.1. This sequence is a reference standard in the RefSeqGene project. On Nov 22, 2018 this sequence version replaced NM_152594.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.119512.1, SRR1660809.82757.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000299084.9/ ENSP00000299084.4 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGAGCGAGG AGACGGCGAC TTCTGACAAC GATAATAGTT ATGCACGAGT GCGAGCTGTG 
GTGATGACCC GAGATGACTC AAGTGGTGGA TGGTTACCAC TTGGAGGGAG TGGACTAAGC 
AGCGTCACTG TCTTCAAAGT CCCTCATCAG GAAGAGAATG GCTGTGCTGA CTTTTTTATC 
CGTGGAGAGC GACTCAGGGA CAAAATGGTG GTTTTGGAAT GTATGCTTAA AAAAGACCTC 
ATTTATAATA AGGTCACTCC AACATTTCAC CACTGGAAGA TTGATGACAA GAAGTTTGGT 
CTTACGTTTC AAAGTCCTGC TGATGCTAGG GCTTTTGATA GAGGTATCCG AAGAGCTATA 
GAGGATATTT CTCAAGGATG CCCCGAATCA AAAAATGAAG CTGAAGGGGC AGATGACTTA 
CAAGCAAATG AAGAGGATTC TTCCAGTTCT CTAGTGAAGG ATCACCTTTT TCAGCAAGAG 
ACAGTTGTTA CCAGTGAGCC TTATAGAAGC TCAAATATAA GACCTTCTCC CTTTGAAGAT 
CTGAATGCCA GAAGAGTCTA CATGCAAAGC CAAGCCAATC AGATAACATT TGGTCAGCCA 
GGCTTGGACA TTCAGAGCAG AAGTATGGAA TACGTACAGC GGCAAATATC CAAGGAATGT 
GGAAGCCTAA AGTCCCAAAA TAGGGTCCCT TTGAAATCAA TCAGACATGT CAGCTTTCAA 
GATGAGGATG AGATTGTCAG AATAAACCCT CGAGATATCT TAATACGTCG CTATGCAGAC 
TACAGACATC CTGACATGTG GAAAAATGAC TTGGAAAGAG ATGATGCTGA TTCCAGTATT 
CAGTTTTCTA AACCAGACAG TAAAAAATCA GACTATCTGT ACTCTTGTGG GGATGAGACT 
AAGTTAAGTT CACCCAAAGA CTCTGTGGTA TTTAAGACGC AGCCTTCCTC ATTAAAAATT 
AAGAAGTCAA AACGAAGAAA AGAGGATGGT GAACGTTCTC GCTGCGTATA CTGCCAGGAA 
AGGTTTAATC ATGAAGAAAA TGTTAGGGGA AAATGTCAGG ATGCTCCAGA CCCTATTAAA 
AGATGCATAT ATCAAGTTAG TTGCATGCTC TGTGCAGAGA GCATGTTGTA TCATTGTATG 
TCAGACTCAG AGGGAGATTT TTCTGATCCC TGTTCGTGTG ACACTAGCGA CGACAAGTTC 
TGCTTGCGAT GGTTAGCCCT GGTAGCTTTG TCTTTCATTG TACCATGTAT GTGCTGCTAC 
GTCCCTTTGA GAATGTGCCA TCGCTGTGGT GAGGCATGTG GTTGCTGTGG TGGGAAACAT 
AAAGCTGCTG GATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_689807.1
CDS	351..1685
Translation	MSEETATSDNDNSYARVRAVVMTRDDSSGGWLPLGGSGLSSVTVFKVPHQEENGCADFFIRGERLRDKMVVLECMLKKDLIYNKVTPTFHHWKIDDKKFGLTFQSPADARAFDRGIRRAIEDISQGCPESKNEAEGADDLQANEEDSSSSLVKDHLFQQETVVTSEPYRSSNIRPSPFEDLNARRVYMQSQANQITFGQPGLDIQSRSMEYVQRQISKECGSLKSQNRVPLKSIRHVSFQDEDEIVRINPRDILIRRYADYRHPDMWKNDLERDDADSSIQFSKPDSKKSDYLYSCGDETKLSSPKDSVVFKTQPSSLKIKKSKRRKEDGERSRCVYCQERFNHEENVRGKCQDAPDPIKRCIYQVSCMLCAESMLYHCMSDSEGDFSDPCSCDTSDDKFCLRWLALVALSFIVPCMCCYVPLRMCHRCGEACGCCGGKHKAAG

Target ORF information:

RefSeq Version	NM_152594.3
Organism	Homo sapiens(human)
Definition	Homo sapiens sprouty related EVH1 domain containing 1 (SPRED1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_152594.3

ORF Insert Sequence:

ATGAGCGAGG AGACGGCGAC TTCTGACAAC GATAATAGTT ATGCACGAGT GCGAGCTGTG 
GTGATGACCC GAGATGACTC AAGTGGTGGA TGGTTACCAC TTGGAGGGAG TGGACTAAGC 
AGCGTCACTG TCTTCAAAGT CCCTCATCAG GAAGAGAATG GCTGTGCTGA CTTTTTTATC 
CGTGGAGAGC GACTCAGGGA CAAAATGGTG GTTTTGGAAT GTATGCTTAA AAAAGACCTC 
ATTTATAATA AGGTCACTCC AACATTTCAC CACTGGAAGA TTGATGACAA GAAGTTTGGT 
CTTACGTTTC AAAGTCCTGC TGATGCTAGG GCTTTTGATA GAGGTATCCG AAGAGCTATA 
GAGGATATTT CTCAAGGATG CCCCGAATCA AAAAATGAAG CTGAAGGGGC AGATGACTTA 
CAAGCAAATG AAGAGGATTC TTCCAGTTCT CTAGTGAAGG ATCACCTTTT TCAGCAAGAG 
ACAGTTGTTA CCAGTGAGCC TTATAGAAGC TCAAATATAA GACCTTCTCC CTTTGAAGAT 
CTGAATGCCA GAAGAGTCTA CATGCAAAGC CAAGCCAATC AGATAACATT TGGTCAGCCA 
GGCTTGGACA TTCAGAGCAG AAGTATGGAA TACGTACAGC GGCAAATATC CAAGGAATGT 
GGAAGCCTAA AGTCCCAAAA TAGGGTCCCT TTGAAATCAA TCAGACATGT CAGCTTTCAA 
GATGAGGATG AGATTGTCAG AATAAACCCT CGAGATATCT TAATACGTCG CTATGCAGAC 
TACAGACATC CTGACATGTG GAAAAATGAC TTGGAAAGAG ATGATGCTGA TTCCAGTATT 
CAGTTTTCTA AACCAGACAG TAAAAAATCA GACTATCTGT ACTCTTGTGG GGATGAGACT 
AAGTTAAGTT CACCCAAAGA CTCTGTGGTA TTTAAGACGC AGCCTTCCTC ATTAAAAATT 
AAGAAGTCAA AACGAAGAAA AGAGGATGGT GAACGTTCTC GCTGCGTATA CTGCCAGGAA 
AGGTTTAATC ATGAAGAAAA TGTTAGGGGA AAATGTCAGG ATGCTCCAGA CCCTATTAAA 
AGATGCATAT ATCAAGTTAG TTGCATGCTC TGTGCAGAGA GCATGTTGTA TCATTGTATG 
TCAGACTCAG AGGGAGATTT TTCTGATCCC TGTTCGTGTG ACACTAGCGA CGACAAGTTC 
TGCTTGCGAT GGTTAGCCCT GGTAGCTTTG TCTTTCATTG TACCATGTAT GTGCTGCTAC 
GTCCCTTTGA GAATGTGCCA TCGCTGTGGT GAGGCATGTG GTTGCTGTGG TGGGAAACAT 
AAAGCTGCTG GATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPRED1 ( NM_152594.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_689807.1 Homo sapiens sprouty related EVH1 domain containing 1 (SPRED1), mRNA.

Price & Availability↑

CloneID	OHu18529
Clone ID Related Accession (Same CDS sequence)	NM_152594.3 , NM_152594.2
Accession Version	NM_152594.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1335bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-05
Organism	Homo sapiens(human)
Product	sprouty-related, EVH1 domain-containing protein 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA405650.1, AY299089.1 and AC069381.8. This sequence is a reference standard in the RefSeqGene project. On May 1, 2008 this sequence version replaced NM_152594.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.119512.1, SRR1660809.82757.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGCGAGG AGACGGCGAC TTCTGACAAC GATAATAGTT ATGCACGAGT GCGAGCTGTG 
GTGATGACCC GAGATGACTC AAGTGGTGGA TGGTTACCAC TTGGAGGGAG TGGACTAAGC 
AGCGTCACTG TCTTCAAAGT CCCTCATCAG GAAGAGAATG GCTGTGCTGA CTTTTTTATC 
CGTGGAGAGC GACTCAGGGA CAAAATGGTG GTTTTGGAAT GTATGCTTAA AAAAGACCTC 
ATTTATAATA AGGTCACTCC AACATTTCAC CACTGGAAGA TTGATGACAA GAAGTTTGGT 
CTTACGTTTC AAAGTCCTGC TGATGCTAGG GCTTTTGATA GAGGTATCCG AAGAGCTATA 
GAGGATATTT CTCAAGGATG CCCCGAATCA AAAAATGAAG CTGAAGGGGC AGATGACTTA 
CAAGCAAATG AAGAGGATTC TTCCAGTTCT CTAGTGAAGG ATCACCTTTT TCAGCAAGAG 
ACAGTTGTTA CCAGTGAGCC TTATAGAAGC TCAAATATAA GACCTTCTCC CTTTGAAGAT 
CTGAATGCCA GAAGAGTCTA CATGCAAAGC CAAGCCAATC AGATAACATT TGGTCAGCCA 
GGCTTGGACA TTCAGAGCAG AAGTATGGAA TACGTACAGC GGCAAATATC CAAGGAATGT 
GGAAGCCTAA AGTCCCAAAA TAGGGTCCCT TTGAAATCAA TCAGACATGT CAGCTTTCAA 
GATGAGGATG AGATTGTCAG AATAAACCCT CGAGATATCT TAATACGTCG CTATGCAGAC 
TACAGACATC CTGACATGTG GAAAAATGAC TTGGAAAGAG ATGATGCTGA TTCCAGTATT 
CAGTTTTCTA AACCAGACAG TAAAAAATCA GACTATCTGT ACTCTTGTGG GGATGAGACT 
AAGTTAAGTT CACCCAAAGA CTCTGTGGTA TTTAAGACGC AGCCTTCCTC ATTAAAAATT 
AAGAAGTCAA AACGAAGAAA AGAGGATGGT GAACGTTCTC GCTGCGTATA CTGCCAGGAA 
AGGTTTAATC ATGAAGAAAA TGTTAGGGGA AAATGTCAGG ATGCTCCAGA CCCTATTAAA 
AGATGCATAT ATCAAGTTAG TTGCATGCTC TGTGCAGAGA GCATGTTGTA TCATTGTATG 
TCAGACTCAG AGGGAGATTT TTCTGATCCC TGTTCGTGTG ACACTAGCGA CGACAAGTTC 
TGCTTGCGAT GGTTAGCCCT GGTAGCTTTG TCTTTCATTG TACCATGTAT GTGCTGCTAC 
GTCCCTTTGA GAATGTGCCA TCGCTGTGGT GAGGCATGTG GTTGCTGTGG TGGGAAACAT 
AAAGCTGCTG GATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_689807.1
CDS	336..1670
Translation	MSEETATSDNDNSYARVRAVVMTRDDSSGGWLPLGGSGLSSVTVFKVPHQEENGCADFFIRGERLRDKMVVLECMLKKDLIYNKVTPTFHHWKIDDKKFGLTFQSPADARAFDRGIRRAIEDISQGCPESKNEAEGADDLQANEEDSSSSLVKDHLFQQETVVTSEPYRSSNIRPSPFEDLNARRVYMQSQANQITFGQPGLDIQSRSMEYVQRQISKECGSLKSQNRVPLKSIRHVSFQDEDEIVRINPRDILIRRYADYRHPDMWKNDLERDDADSSIQFSKPDSKKSDYLYSCGDETKLSSPKDSVVFKTQPSSLKIKKSKRRKEDGERSRCVYCQERFNHEENVRGKCQDAPDPIKRCIYQVSCMLCAESMLYHCMSDSEGDFSDPCSCDTSDDKFCLRWLALVALSFIVPCMCCYVPLRMCHRCGEACGCCGGKHKAAG

Target ORF information:

RefSeq Version	NM_152594.2
Organism	Homo sapiens(human)
Definition	Homo sapiens sprouty related EVH1 domain containing 1 (SPRED1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_152594.2

ORF Insert Sequence:

ATGAGCGAGG AGACGGCGAC TTCTGACAAC GATAATAGTT ATGCACGAGT GCGAGCTGTG 
GTGATGACCC GAGATGACTC AAGTGGTGGA TGGTTACCAC TTGGAGGGAG TGGACTAAGC 
AGCGTCACTG TCTTCAAAGT CCCTCATCAG GAAGAGAATG GCTGTGCTGA CTTTTTTATC 
CGTGGAGAGC GACTCAGGGA CAAAATGGTG GTTTTGGAAT GTATGCTTAA AAAAGACCTC 
ATTTATAATA AGGTCACTCC AACATTTCAC CACTGGAAGA TTGATGACAA GAAGTTTGGT 
CTTACGTTTC AAAGTCCTGC TGATGCTAGG GCTTTTGATA GAGGTATCCG AAGAGCTATA 
GAGGATATTT CTCAAGGATG CCCCGAATCA AAAAATGAAG CTGAAGGGGC AGATGACTTA 
CAAGCAAATG AAGAGGATTC TTCCAGTTCT CTAGTGAAGG ATCACCTTTT TCAGCAAGAG 
ACAGTTGTTA CCAGTGAGCC TTATAGAAGC TCAAATATAA GACCTTCTCC CTTTGAAGAT 
CTGAATGCCA GAAGAGTCTA CATGCAAAGC CAAGCCAATC AGATAACATT TGGTCAGCCA 
GGCTTGGACA TTCAGAGCAG AAGTATGGAA TACGTACAGC GGCAAATATC CAAGGAATGT 
GGAAGCCTAA AGTCCCAAAA TAGGGTCCCT TTGAAATCAA TCAGACATGT CAGCTTTCAA 
GATGAGGATG AGATTGTCAG AATAAACCCT CGAGATATCT TAATACGTCG CTATGCAGAC 
TACAGACATC CTGACATGTG GAAAAATGAC TTGGAAAGAG ATGATGCTGA TTCCAGTATT 
CAGTTTTCTA AACCAGACAG TAAAAAATCA GACTATCTGT ACTCTTGTGG GGATGAGACT 
AAGTTAAGTT CACCCAAAGA CTCTGTGGTA TTTAAGACGC AGCCTTCCTC ATTAAAAATT 
AAGAAGTCAA AACGAAGAAA AGAGGATGGT GAACGTTCTC GCTGCGTATA CTGCCAGGAA 
AGGTTTAATC ATGAAGAAAA TGTTAGGGGA AAATGTCAGG ATGCTCCAGA CCCTATTAAA 
AGATGCATAT ATCAAGTTAG TTGCATGCTC TGTGCAGAGA GCATGTTGTA TCATTGTATG 
TCAGACTCAG AGGGAGATTT TTCTGATCCC TGTTCGTGTG ACACTAGCGA CGACAAGTTC 
TGCTTGCGAT GGTTAGCCCT GGTAGCTTTG TCTTTCATTG TACCATGTAT GTGCTGCTAC 
GTCCCTTTGA GAATGTGCCA TCGCTGTGGT GAGGCATGTG GTTGCTGTGG TGGGAAACAT 
AAAGCTGCTG GATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPRED1 ( XM_005254202.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_005254259.1 Homo sapiens sprouty related EVH1 domain containing 1 (SPRED1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu48143
Clone ID Related Accession (Same CDS sequence)	XM_005254202.3
Accession Version	XM_005254202.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1371bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	sprouty-related, EVH1 domain-containing protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005254202.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCGAGG AGACGGCGAC TTCTGACAAC GAGTGTCAGG GGCATGATGA TGGAATCTGC 
TTTTTTCATA ATAGTTATGC ACGAGTGCGA GCTGTGGTGA TGACCCGAGA TGACTCAAGT 
GGTGGATGGT TACCACTTGG AGGGAGTGGA CTAAGCAGCG TCACTGTCTT CAAAGTCCCT 
CATCAGGAAG AGAATGGCTG TGCTGACTTT TTTATCCGTG GAGAGCGACT CAGGGACAAA 
ATGGTGGTTT TGGAATGTAT GCTTAAAAAA GACCTCATTT ATAATAAGGT CACTCCAACA 
TTTCACCACT GGAAGATTGA TGACAAGAAG TTTGGTCTTA CGTTTCAAAG TCCTGCTGAT 
GCTAGGGCTT TTGATAGAGG TATCCGAAGA GCTATAGAGG ATATTTCTCA AGGATGCCCC 
GAATCAAAAA ATGAAGCTGA AGGGGCAGAT GACTTACAAG CAAATGAAGA GGATTCTTCC 
AGTTCTCTAG TGAAGGATCA CCTTTTTCAG CAAGAGACAG TTGTTACCAG TGAGCCTTAT 
AGAAGCTCAA ATATAAGACC TTCTCCCTTT GAAGATCTGA ATGCCAGAAG AGTCTACATG 
CAAAGCCAAG CCAATCAGAT AACATTTGGT CAGCCAGGCT TGGACATTCA GAGCAGAAGT 
ATGGAATACG TACAGCGGCA AATATCCAAG GAATGTGGAA GCCTAAAGTC CCAAAATAGG 
GTCCCTTTGA AATCAATCAG ACATGTCAGC TTTCAAGATG AGGATGAGAT TGTCAGAATA 
AACCCTCGAG ATATCTTAAT ACGTCGCTAT GCAGACTACA GACATCCTGA CATGTGGAAA 
AATGACTTGG AAAGAGATGA TGCTGATTCC AGTATTCAGT TTTCTAAACC AGACAGTAAA 
AAATCAGACT ATCTGTACTC TTGTGGGGAT GAGACTAAGT TAAGTTCACC CAAAGACTCT 
GTGGTATTTA AGACGCAGCC TTCCTCATTA AAAATTAAGA AGTCAAAACG AAGAAAAGAG 
GATGGTGAAC GTTCTCGCTG CGTATACTGC CAGGAAAGGT TTAATCATGA AGAAAATGTT 
AGGGGAAAAT GTCAGGATGC TCCAGACCCT ATTAAAAGAT GCATATATCA AGTTAGTTGC 
ATGCTCTGTG CAGAGAGCAT GTTGTATCAT TGTATGTCAG ACTCAGAGGG AGATTTTTCT 
GATCCCTGTT CGTGTGACAC TAGCGACGAC AAGTTCTGCT TGCGATGGTT AGCCCTGGTA 
GCTTTGTCTT TCATTGTACC ATGTATGTGC TGCTACGTCC CTTTGAGAAT GTGCCATCGC 
TGTGGTGAGG CATGTGGTTG CTGTGGTGGG AAACATAAAG CTGCTGGATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005254259.1
CDS	1100..2470
Translation	MSEETATSDNECQGHDDGICFFHNSYARVRAVVMTRDDSSGGWLPLGGSGLSSVTVFKVPHQEENGCADFFIRGERLRDKMVVLECMLKKDLIYNKVTPTFHHWKIDDKKFGLTFQSPADARAFDRGIRRAIEDISQGCPESKNEAEGADDLQANEEDSSSSLVKDHLFQQETVVTSEPYRSSNIRPSPFEDLNARRVYMQSQANQITFGQPGLDIQSRSMEYVQRQISKECGSLKSQNRVPLKSIRHVSFQDEDEIVRINPRDILIRRYADYRHPDMWKNDLERDDADSSIQFSKPDSKKSDYLYSCGDETKLSSPKDSVVFKTQPSSLKIKKSKRRKEDGERSRCVYCQERFNHEENVRGKCQDAPDPIKRCIYQVSCMLCAESMLYHCMSDSEGDFSDPCSCDTSDDKFCLRWLALVALSFIVPCMCCYVPLRMCHRCGEACGCCGGKHKAAG

Target ORF information:

RefSeq Version	XM_005254202.3
Organism	Homo sapiens(human)
Definition	Homo sapiens sprouty related EVH1 domain containing 1 (SPRED1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005254202.3

ORF Insert Sequence:

ATGAGCGAGG AGACGGCGAC TTCTGACAAC GAGTGTCAGG GGCATGATGA TGGAATCTGC 
TTTTTTCATA ATAGTTATGC ACGAGTGCGA GCTGTGGTGA TGACCCGAGA TGACTCAAGT 
GGTGGATGGT TACCACTTGG AGGGAGTGGA CTAAGCAGCG TCACTGTCTT CAAAGTCCCT 
CATCAGGAAG AGAATGGCTG TGCTGACTTT TTTATCCGTG GAGAGCGACT CAGGGACAAA 
ATGGTGGTTT TGGAATGTAT GCTTAAAAAA GACCTCATTT ATAATAAGGT CACTCCAACA 
TTTCACCACT GGAAGATTGA TGACAAGAAG TTTGGTCTTA CGTTTCAAAG TCCTGCTGAT 
GCTAGGGCTT TTGATAGAGG TATCCGAAGA GCTATAGAGG ATATTTCTCA AGGATGCCCC 
GAATCAAAAA ATGAAGCTGA AGGGGCAGAT GACTTACAAG CAAATGAAGA GGATTCTTCC 
AGTTCTCTAG TGAAGGATCA CCTTTTTCAG CAAGAGACAG TTGTTACCAG TGAGCCTTAT 
AGAAGCTCAA ATATAAGACC TTCTCCCTTT GAAGATCTGA ATGCCAGAAG AGTCTACATG 
CAAAGCCAAG CCAATCAGAT AACATTTGGT CAGCCAGGCT TGGACATTCA GAGCAGAAGT 
ATGGAATACG TACAGCGGCA AATATCCAAG GAATGTGGAA GCCTAAAGTC CCAAAATAGG 
GTCCCTTTGA AATCAATCAG ACATGTCAGC TTTCAAGATG AGGATGAGAT TGTCAGAATA 
AACCCTCGAG ATATCTTAAT ACGTCGCTAT GCAGACTACA GACATCCTGA CATGTGGAAA 
AATGACTTGG AAAGAGATGA TGCTGATTCC AGTATTCAGT TTTCTAAACC AGACAGTAAA 
AAATCAGACT ATCTGTACTC TTGTGGGGAT GAGACTAAGT TAAGTTCACC CAAAGACTCT 
GTGGTATTTA AGACGCAGCC TTCCTCATTA AAAATTAAGA AGTCAAAACG AAGAAAAGAG 
GATGGTGAAC GTTCTCGCTG CGTATACTGC CAGGAAAGGT TTAATCATGA AGAAAATGTT 
AGGGGAAAAT GTCAGGATGC TCCAGACCCT ATTAAAAGAT GCATATATCA AGTTAGTTGC 
ATGCTCTGTG CAGAGAGCAT GTTGTATCAT TGTATGTCAG ACTCAGAGGG AGATTTTTCT 
GATCCCTGTT CGTGTGACAC TAGCGACGAC AAGTTCTGCT TGCGATGGTT AGCCCTGGTA 
GCTTTGTCTT TCATTGTACC ATGTATGTGC TGCTACGTCC CTTTGAGAAT GTGCCATCGC 
TGTGGTGAGG CATGTGGTTG CTGTGGTGGG AAACATAAAG CTGCTGGATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPRED1 ( XM_011521289.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011519591.1 Homo sapiens sprouty related EVH1 domain containing 1 (SPRED1), transcript variant X2, mRNA.

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CloneID	OHu77141
Clone ID Related Accession (Same CDS sequence)	XM_011521289.2 , XM_011521289.3
Accession Version	XM_011521289.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1272bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	sprouty-related, EVH1 domain-containing protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011521289.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCCGAG ATGACTCAAG TGGTGGATGG TTACCACTTG GAGGGAGTGG ACTAAGCAGC 
GTCACTGTCT TCAAAGTCCC TCATCAGGAA GAGAATGGCT GTGCTGACTT TTTTATCCGT 
GGAGAGCGAC TCAGGGACAA AATGGTGGTT TTGGAATGTA TGCTTAAAAA AGACCTCATT 
TATAATAAGG TCACTCCAAC ATTTCACCAC TGGAAGATTG ATGACAAGAA GTTTGGTCTT 
ACGTTTCAAA GTCCTGCTGA TGCTAGGGCT TTTGATAGAG GTATCCGAAG AGCTATAGAG 
GATATTTCTC AAGGATGCCC CGAATCAAAA AATGAAGCTG AAGGGGCAGA TGACTTACAA 
GCAAATGAAG AGGATTCTTC CAGTTCTCTA GTGAAGGATC ACCTTTTTCA GCAAGAGACA 
GTTGTTACCA GTGAGCCTTA TAGAAGCTCA AATATAAGAC CTTCTCCCTT TGAAGATCTG 
AATGCCAGAA GAGTCTACAT GCAAAGCCAA GCCAATCAGA TAACATTTGG TCAGCCAGGC 
TTGGACATTC AGAGCAGAAG TATGGAATAC GTACAGCGGC AAATATCCAA GGAATGTGGA 
AGCCTAAAGT CCCAAAATAG GGTCCCTTTG AAATCAATCA GACATGTCAG CTTTCAAGAT 
GAGGATGAGA TTGTCAGAAT AAACCCTCGA GATATCTTAA TACGTCGCTA TGCAGACTAC 
AGACATCCTG ACATGTGGAA AAATGACTTG GAAAGAGATG ATGCTGATTC CAGTATTCAG 
TTTTCTAAAC CAGACAGTAA AAAATCAGAC TATCTGTACT CTTGTGGGGA TGAGACTAAG 
TTAAGTTCAC CCAAAGACTC TGTGGTATTT AAGACGCAGC CTTCCTCATT AAAAATTAAG 
AAGTCAAAAC GAAGAAAAGA GGATGGTGAA CGTTCTCGCT GCGTATACTG CCAGGAAAGG 
TTTAATCATG AAGAAAATGT TAGGGGAAAA TGTCAGGATG CTCCAGACCC TATTAAAAGA 
TGCATATATC AAGTTAGTTG CATGCTCTGT GCAGAGAGCA TGTTGTATCA TTGTATGTCA 
GACTCAGAGG GAGATTTTTC TGATCCCTGT TCGTGTGACA CTAGCGACGA CAAGTTCTGC 
TTGCGATGGT TAGCCCTGGT AGCTTTGTCT TTCATTGTAC CATGTATGTG CTGCTACGTC 
CCTTTGAGAA TGTGCCATCG CTGTGGTGAG GCATGTGGTT GCTGTGGTGG GAAACATAAA 
GCTGCTGGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011519591.1
CDS	96..1367
Translation	MTRDDSSGGWLPLGGSGLSSVTVFKVPHQEENGCADFFIRGERLRDKMVVLECMLKKDLIYNKVTPTFHHWKIDDKKFGLTFQSPADARAFDRGIRRAIEDISQGCPESKNEAEGADDLQANEEDSSSSLVKDHLFQQETVVTSEPYRSSNIRPSPFEDLNARRVYMQSQANQITFGQPGLDIQSRSMEYVQRQISKECGSLKSQNRVPLKSIRHVSFQDEDEIVRINPRDILIRRYADYRHPDMWKNDLERDDADSSIQFSKPDSKKSDYLYSCGDETKLSSPKDSVVFKTQPSSLKIKKSKRRKEDGERSRCVYCQERFNHEENVRGKCQDAPDPIKRCIYQVSCMLCAESMLYHCMSDSEGDFSDPCSCDTSDDKFCLRWLALVALSFIVPCMCCYVPLRMCHRCGEACGCCGGKHKAAG

Target ORF information:

RefSeq Version	XM_011521289.2
Organism	Homo sapiens(human)
Definition	Homo sapiens sprouty related EVH1 domain containing 1 (SPRED1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011521289.2

ORF Insert Sequence:

ATGACCCGAG ATGACTCAAG TGGTGGATGG TTACCACTTG GAGGGAGTGG ACTAAGCAGC 
GTCACTGTCT TCAAAGTCCC TCATCAGGAA GAGAATGGCT GTGCTGACTT TTTTATCCGT 
GGAGAGCGAC TCAGGGACAA AATGGTGGTT TTGGAATGTA TGCTTAAAAA AGACCTCATT 
TATAATAAGG TCACTCCAAC ATTTCACCAC TGGAAGATTG ATGACAAGAA GTTTGGTCTT 
ACGTTTCAAA GTCCTGCTGA TGCTAGGGCT TTTGATAGAG GTATCCGAAG AGCTATAGAG 
GATATTTCTC AAGGATGCCC CGAATCAAAA AATGAAGCTG AAGGGGCAGA TGACTTACAA 
GCAAATGAAG AGGATTCTTC CAGTTCTCTA GTGAAGGATC ACCTTTTTCA GCAAGAGACA 
GTTGTTACCA GTGAGCCTTA TAGAAGCTCA AATATAAGAC CTTCTCCCTT TGAAGATCTG 
AATGCCAGAA GAGTCTACAT GCAAAGCCAA GCCAATCAGA TAACATTTGG TCAGCCAGGC 
TTGGACATTC AGAGCAGAAG TATGGAATAC GTACAGCGGC AAATATCCAA GGAATGTGGA 
AGCCTAAAGT CCCAAAATAG GGTCCCTTTG AAATCAATCA GACATGTCAG CTTTCAAGAT 
GAGGATGAGA TTGTCAGAAT AAACCCTCGA GATATCTTAA TACGTCGCTA TGCAGACTAC 
AGACATCCTG ACATGTGGAA AAATGACTTG GAAAGAGATG ATGCTGATTC CAGTATTCAG 
TTTTCTAAAC CAGACAGTAA AAAATCAGAC TATCTGTACT CTTGTGGGGA TGAGACTAAG 
TTAAGTTCAC CCAAAGACTC TGTGGTATTT AAGACGCAGC CTTCCTCATT AAAAATTAAG 
AAGTCAAAAC GAAGAAAAGA GGATGGTGAA CGTTCTCGCT GCGTATACTG CCAGGAAAGG 
TTTAATCATG AAGAAAATGT TAGGGGAAAA TGTCAGGATG CTCCAGACCC TATTAAAAGA 
TGCATATATC AAGTTAGTTG CATGCTCTGT GCAGAGAGCA TGTTGTATCA TTGTATGTCA 
GACTCAGAGG GAGATTTTTC TGATCCCTGT TCGTGTGACA CTAGCGACGA CAAGTTCTGC 
TTGCGATGGT TAGCCCTGGT AGCTTTGTCT TTCATTGTAC CATGTATGTG CTGCTACGTC 
CCTTTGAGAA TGTGCCATCG CTGTGGTGAG GCATGTGGTT GCTGTGGTGG GAAACATAAA 
GCTGCTGGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPRED1 ( XM_011521289.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011519591.1 Homo sapiens sprouty related EVH1 domain containing 1 (SPRED1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu77141
Clone ID Related Accession (Same CDS sequence)	XM_011521289.2 , XM_011521289.3
Accession Version	XM_011521289.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1272bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	sprouty-related, EVH1 domain-containing protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011521289.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCCGAG ATGACTCAAG TGGTGGATGG TTACCACTTG GAGGGAGTGG ACTAAGCAGC 
GTCACTGTCT TCAAAGTCCC TCATCAGGAA GAGAATGGCT GTGCTGACTT TTTTATCCGT 
GGAGAGCGAC TCAGGGACAA AATGGTGGTT TTGGAATGTA TGCTTAAAAA AGACCTCATT 
TATAATAAGG TCACTCCAAC ATTTCACCAC TGGAAGATTG ATGACAAGAA GTTTGGTCTT 
ACGTTTCAAA GTCCTGCTGA TGCTAGGGCT TTTGATAGAG GTATCCGAAG AGCTATAGAG 
GATATTTCTC AAGGATGCCC CGAATCAAAA AATGAAGCTG AAGGGGCAGA TGACTTACAA 
GCAAATGAAG AGGATTCTTC CAGTTCTCTA GTGAAGGATC ACCTTTTTCA GCAAGAGACA 
GTTGTTACCA GTGAGCCTTA TAGAAGCTCA AATATAAGAC CTTCTCCCTT TGAAGATCTG 
AATGCCAGAA GAGTCTACAT GCAAAGCCAA GCCAATCAGA TAACATTTGG TCAGCCAGGC 
TTGGACATTC AGAGCAGAAG TATGGAATAC GTACAGCGGC AAATATCCAA GGAATGTGGA 
AGCCTAAAGT CCCAAAATAG GGTCCCTTTG AAATCAATCA GACATGTCAG CTTTCAAGAT 
GAGGATGAGA TTGTCAGAAT AAACCCTCGA GATATCTTAA TACGTCGCTA TGCAGACTAC 
AGACATCCTG ACATGTGGAA AAATGACTTG GAAAGAGATG ATGCTGATTC CAGTATTCAG 
TTTTCTAAAC CAGACAGTAA AAAATCAGAC TATCTGTACT CTTGTGGGGA TGAGACTAAG 
TTAAGTTCAC CCAAAGACTC TGTGGTATTT AAGACGCAGC CTTCCTCATT AAAAATTAAG 
AAGTCAAAAC GAAGAAAAGA GGATGGTGAA CGTTCTCGCT GCGTATACTG CCAGGAAAGG 
TTTAATCATG AAGAAAATGT TAGGGGAAAA TGTCAGGATG CTCCAGACCC TATTAAAAGA 
TGCATATATC AAGTTAGTTG CATGCTCTGT GCAGAGAGCA TGTTGTATCA TTGTATGTCA 
GACTCAGAGG GAGATTTTTC TGATCCCTGT TCGTGTGACA CTAGCGACGA CAAGTTCTGC 
TTGCGATGGT TAGCCCTGGT AGCTTTGTCT TTCATTGTAC CATGTATGTG CTGCTACGTC 
CCTTTGAGAA TGTGCCATCG CTGTGGTGAG GCATGTGGTT GCTGTGGTGG GAAACATAAA 
GCTGCTGGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011519591.1
CDS	869..2140
Translation	MTRDDSSGGWLPLGGSGLSSVTVFKVPHQEENGCADFFIRGERLRDKMVVLECMLKKDLIYNKVTPTFHHWKIDDKKFGLTFQSPADARAFDRGIRRAIEDISQGCPESKNEAEGADDLQANEEDSSSSLVKDHLFQQETVVTSEPYRSSNIRPSPFEDLNARRVYMQSQANQITFGQPGLDIQSRSMEYVQRQISKECGSLKSQNRVPLKSIRHVSFQDEDEIVRINPRDILIRRYADYRHPDMWKNDLERDDADSSIQFSKPDSKKSDYLYSCGDETKLSSPKDSVVFKTQPSSLKIKKSKRRKEDGERSRCVYCQERFNHEENVRGKCQDAPDPIKRCIYQVSCMLCAESMLYHCMSDSEGDFSDPCSCDTSDDKFCLRWLALVALSFIVPCMCCYVPLRMCHRCGEACGCCGGKHKAAG

Target ORF information:

RefSeq Version	XM_011521289.3
Organism	Homo sapiens(human)
Definition	Homo sapiens sprouty related EVH1 domain containing 1 (SPRED1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011521289.3

ORF Insert Sequence:

ATGACCCGAG ATGACTCAAG TGGTGGATGG TTACCACTTG GAGGGAGTGG ACTAAGCAGC 
GTCACTGTCT TCAAAGTCCC TCATCAGGAA GAGAATGGCT GTGCTGACTT TTTTATCCGT 
GGAGAGCGAC TCAGGGACAA AATGGTGGTT TTGGAATGTA TGCTTAAAAA AGACCTCATT 
TATAATAAGG TCACTCCAAC ATTTCACCAC TGGAAGATTG ATGACAAGAA GTTTGGTCTT 
ACGTTTCAAA GTCCTGCTGA TGCTAGGGCT TTTGATAGAG GTATCCGAAG AGCTATAGAG 
GATATTTCTC AAGGATGCCC CGAATCAAAA AATGAAGCTG AAGGGGCAGA TGACTTACAA 
GCAAATGAAG AGGATTCTTC CAGTTCTCTA GTGAAGGATC ACCTTTTTCA GCAAGAGACA 
GTTGTTACCA GTGAGCCTTA TAGAAGCTCA AATATAAGAC CTTCTCCCTT TGAAGATCTG 
AATGCCAGAA GAGTCTACAT GCAAAGCCAA GCCAATCAGA TAACATTTGG TCAGCCAGGC 
TTGGACATTC AGAGCAGAAG TATGGAATAC GTACAGCGGC AAATATCCAA GGAATGTGGA 
AGCCTAAAGT CCCAAAATAG GGTCCCTTTG AAATCAATCA GACATGTCAG CTTTCAAGAT 
GAGGATGAGA TTGTCAGAAT AAACCCTCGA GATATCTTAA TACGTCGCTA TGCAGACTAC 
AGACATCCTG ACATGTGGAA AAATGACTTG GAAAGAGATG ATGCTGATTC CAGTATTCAG 
TTTTCTAAAC CAGACAGTAA AAAATCAGAC TATCTGTACT CTTGTGGGGA TGAGACTAAG 
TTAAGTTCAC CCAAAGACTC TGTGGTATTT AAGACGCAGC CTTCCTCATT AAAAATTAAG 
AAGTCAAAAC GAAGAAAAGA GGATGGTGAA CGTTCTCGCT GCGTATACTG CCAGGAAAGG 
TTTAATCATG AAGAAAATGT TAGGGGAAAA TGTCAGGATG CTCCAGACCC TATTAAAAGA 
TGCATATATC AAGTTAGTTG CATGCTCTGT GCAGAGAGCA TGTTGTATCA TTGTATGTCA 
GACTCAGAGG GAGATTTTTC TGATCCCTGT TCGTGTGACA CTAGCGACGA CAAGTTCTGC 
TTGCGATGGT TAGCCCTGGT AGCTTTGTCT TTCATTGTAC CATGTATGTG CTGCTACGTC 
CCTTTGAGAA TGTGCCATCG CTGTGGTGAG GCATGTGGTT GCTGTGGTGG GAAACATAAA 
GCTGCTGGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The first Slovak Legius syndrome patient carrying the SPRED1 gene mutation.
General physiology and biophysics36(2)205-210(2017 Apr)
Sekelska M,Briatkova L,Olcak T,Bolcekova A,Ilencikova D,Kadasi L,Zatkova A

Potential diagnostic implications of miR-144 overexpression in human oesophageal cancer.
The Indian journal of medical research143(Supplement)S91-S103(2016 May)
Sharma P,Saraya A,Sharma R

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SPRED1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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