DGKH cDNA ORF clone, Homo sapiens(human)

The following DGKH gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DGKH cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu28739 NM_152910.6
Latest version!
Homo sapiens diacylglycerol kinase eta (DGKH), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$399.50-$559.30
$799.00
OHu28739 NM_001204504.2
Latest version!
Homo sapiens diacylglycerol kinase eta (DGKH), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$399.50-$559.30
$799.00
OHu28741 NM_001204506.3
Latest version!
Homo sapiens diacylglycerol kinase eta (DGKH), transcript variant 5, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$399.50-$559.30
$799.00
OHu28845 NM_178009.5
Latest version!
Homo sapiens diacylglycerol kinase eta (DGKH), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$459.50-$643.30
$919.00
OHu28742 NM_001297429.2
Latest version!
Homo sapiens diacylglycerol kinase eta (DGKH), transcript variant 6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $475.30
$679.00
OHu28740 NM_001204505.3
Latest version!
Homo sapiens diacylglycerol kinase eta (DGKH), transcript variant 4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $559.30
$799.00
View Old Accession Versions >>
OHu28845 NM_178009.4 Homo sapiens diacylglycerol kinase eta (DGKH), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$459.50-$643.30
$919.00
OHu28740 NM_001204505.2 Homo sapiens diacylglycerol kinase eta (DGKH), transcript variant 4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $559.30
$799.00
OHu28739 NM_152910.5 Homo sapiens diacylglycerol kinase eta (DGKH), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$399.50-$559.30
$799.00
OHu28742 NM_001297429.1 Homo sapiens diacylglycerol kinase eta (DGKH), transcript variant 6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $475.30
$679.00
OHu28741 NM_001204506.2 Homo sapiens diacylglycerol kinase eta (DGKH), transcript variant 5, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$399.50-$559.30
$799.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu28739
    Clone ID Related Accession (Same CDS sequence) NM_152910.5 , NM_152910.6 , NM_001204504.2
    Accession Version NM_152910.5 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3495bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-01-26
    Organism Homo sapiens(human)
    Product diacylglycerol kinase eta isoform 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DR002010.1, AB078967.1, AK302631.1, BC043292.2, AL136527.9 and BM978856.1. On Jul 2, 2014 this sequence version replaced NM_152910.4. Transcript Variant: This variant (1) encodes isoform 1. Variants 1 and 3 encode the same isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.216359.1, AB078967.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2142348 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGCAGGGG CCGGAGGCCA GCACCACCCT CCGGGCGCCG CTGGAGGAGC GGCCGCCGGA 
    GCCGGCGCCG CGGTCACCTC CGCCGCTGCC TCGGCGGGGC CGGGAGAGGA TTCGTCTGAC
    AGCGAAGCGG AGCAAGAGGG ACCCCAGAAA CTGATCCGCA AAGTGTCTAC CTCGGGGCAG
    ATCCGGACCA AGACCAGTAT TAAAGAGGGA CAGCTATTGA AGCAAACCAG TTCTTTCCAA
    AGGTGGAAAA AGCGATACTT CAAACTTCGA GGCCGCACCC TTTACTATGC AAAGGACTCA
    AAGTCTCTGA TATTTGATGA AGTTGACCTC TCAGATGCTA GTGTAGCTGA AGCAAGCACG
    AAAAATGCTA ACAACAGCTT CACGATCATC ACTCCATTCA GAAGGCTAAT GCTGTGTGCT
    GAGAACAGAA AGGAGATGGA GGATTGGATC AGCTCACTGA AGTCTGTACA GACCAGAGAA
    CCCTACGAGG TGGCCCAGTT TAATGTGGAA CATTTCTCAG GGATGCACAA CTGGTACGCC
    TGCTCCCACG CCCGACCCAC CTTCTGTAAC GTGTGCAGAG AGAGTCTTTC TGGAGTCACC
    TCCCATGGCC TGTCCTGCGA AGTGTGTAAA TTCAAGGCTC ACAAAAGATG TGCAGTGAGA
    GCAACAAATA ACTGTAAATG GACTACCCTG GCCTCCATCG GGAAGGACAT TATAGAAGAT
    GAAGATGGCG TCGCGATGCC TCACCAGTGG CTTGAGGGCA ACCTGCCTGT AAGTGCCAAG
    TGTGCTGTCT GCGACAAAAC ATGTGGCAGT GTTCTCCGTC TACAGGATTG GAAATGCCTT
    TGGTGTAAGA CAATGGTACA CACTGCCTGC AAAGATTTAT ACCATCCAAT ATGTCCACTT
    GGTCAATGTA AAGTATCTAT CATACCTCCA ATTGCACTAA ACAGCACCGA TTCCGATGGT
    TTCTGTAGAG CAACATTTTC GTTCTGTGTT AGTCCTCTAT TGGTTTTTGT CAATTCTAAG
    AGTGGAGATA ATCAGGGAGT AAAGTTCCTC CGTCGCTTTA AACAGTTGCT AAATCCGGCT
    CAGGTGTTTG ATTTAATGAA TGGAGGTCCT CATTTAGGTT TAAGATTATT TCAGAAGTTT
    GACAATTTCC GGATTCTTGT TTGTGGAGGC GATGGAAGTG TAGGTTGGGT TTTGTCAGAA
    ATCGATAAGC TCAACTTGAA TAAACAGTGT CAGCTGGGAG TGTTGCCTTT GGGTACAGGA
    AATGACCTTG CCCGAGTTCT TGGCTGGGGA GGTTCATATG ACGATGACAC CCAACTTCCT
    CAGATCCTAG AGAAACTGGA ACGAGCCAGT ACCAAAATGT TGGACAGGTG GAGTATAATG
    ACATATGAAC TCAAATTGCC ACCAAAAGCT TCCCTACTTC CAGGACCTCC AGAAGCATCT
    GAAGAATTTT ATATGACGAT TTATGAAGAC TCAGTTGCAA CGCATCTTAC AAAAATCCTC
    AATTCTGATG AACATGCAGT GGTCATATCT TCTGCCAAGA CGCTATGTGA AACTGTAAAG
    GACTTCGTTG CCAAAGTAGA AAAGACGTAT GACAAAACCT TGGAAAATGC CGTTGTAGCT
    GATGCCGTGG CCAGTAAATG TTCAGTCCTA AACGAGAAGC TCGAACAACT GCTGCAGGCT
    TTGCACACAG ATTCCCAGGC TGCGCCTGTT CTCCCTGGCC TCAGCCCTCT CATTGTGGAA
    GAAGATGCTG TGGAATCGTC CAGTGAAGAG TCCCTGGGTG AAAGCAAAGA GCAGCTTGGG
    GATGACGTTA CAAAACCTTC CTCCCAGAAA GCCGTCAAAC CAAGGGAAAT CATGTTGCGG
    GCAAATAGTT TAAAGAAAGC AGTGAGGCAA GTCATTGAGG AAGCCGGAAA AGTTATGGAT
    GACCCGACAG TTCACCCCTG TGAACCAGCT AATCAGTCCT CTGATTATGA CAGCACAGAA
    ACAGATGAAT CTAAGGAGGA AGCTAAAGAT GATGGTGCCA AAGAATCAAT AACTGTTAAA
    ACTGCACCTC GGTCTCCAGA TGCCCGGGCA AGTTATGGCC ATTCCCAAAC TGATTCTGTC
    CCTGGTCCAG CTGTGGCAGC CAGCAAAGAA AACCTCCCTG TGCTCAATAC CAGAATAATC
    TGCCCAGGTT TAAGAGCAGG ACTGGCTGCC TCAATTGCTG GGAGTTCGAT TATCAACAAA
    ATGTTACTGG CAAACATTGA TCCTTTTGGT GCCACGCCGT TTATTGACCC GGATCTAGAT
    TCCGTAGATG GATATTCAGA AAAATGTGTC ATGAACAATT ACTTTGGGAT TGGATTAGAT
    GCAAAAATTT CATTAGAATT TAATAATAAA AGAGAGGAGC ACCCTGAAAA ATGCAGGAGC
    CGAACTAAAA ACTTGATGTG GTATGGAGTC CTTGGAACCC GGGAGTTATT ACAGAGATCG
    TACAAGAATT TAGAACAAAG GGTTCAACTT GAGTGTGATG GGCAGTATAT TCCTCTTCCC
    AGCTTGCAAG GCATAGCCGT GTTGAACATT CCCAGCTATG CTGGAGGCAC TAACTTTTGG
    GGTGGAACTA AAGAGGATGA TATATTTGCT GCACCATCCT TTGATGACAA GATCCTGGAA
    GTTGTAGCAA TATTTGATAG CATGCAAATG GCAGTTTCAA GGGTCATTAA ACTGCAGCAT
    CATCGAATAG CCCAGTGCCG TACAGTGAAA ATCACTATAT TTGGTGACGA AGGAGTCCCA
    GTGCAAGTGG ATGGTGAAGC GTGGGTTCAG CCTCCAGGGA TTATCAAAAT TGTGCACAAA
    AACAGAGCAC AAATGCTAAC AAGGGACAGA GCCTTTGAGA GCACTCTGAA ATCTTGGGAA
    GATAAGCAGA AGTGTGATTC TGGTAAACCA GTTCTCCGAA CCCATTTGTA CATCCATCAC
    GCCATTGACT TGGCAACAGA AGAGGTGTCG CAGATGCAGC TATGCTCCCA GGCTGCAGAG
    GAGCTCATTA CTAGGATATG TGACGCAGCC ACAATTCACT GTCTTTTGGA GCAAGAACTG
    GCCCATGCTG TGAATGCCTG CTCCCATGCC CTGAATAAAG CCAACCCAAG GTGCCCGGAG
    AGTCTTACAA GAGACACTGC CACTGAAATA GCCATCAATG TGAAGGCGCT GTATAATGAA
    ACAGAATCTT TGCTAGTTGG CAGGGTTCCT TTGCAGCTGG AATCGCCACA TGAAGAGCGA
    GTATCCAATG CCTTACACTC TGTGGAGGTG GAATTACAGA AACTGACAGA GATTCCTTGG
    CTTTATTATA TCTTACACCC AAATGAGGAT GAGGAACCTC CTATGGATTG CACCAAAAGG
    AACAACAGAA GCACCGTATT TCGAATAGTG CCAAAGTTTA AAAAGGAAAA GGTTCAGAAG
    CAGAAGACAA GTTCACAGCC TGGATCTGGG GATACCGAAA GTGGGTCATG TGAAGCGAAT
    TCTCCAGGGA ATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_690874.2
    CDS67..3561
    Translation

    Target ORF information:

    RefSeq Version NM_152910.5
    Organism Homo sapiens(human)
    Definition Homo sapiens diacylglycerol kinase eta (DGKH), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_152910.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGCAGGGG CCGGAGGCCA GCACCACCCT CCGGGCGCCG CTGGAGGAGC GGCCGCCGGA 
    GCCGGCGCCG CGGTCACCTC CGCCGCTGCC TCGGCGGGGC CGGGAGAGGA TTCGTCTGAC
    AGCGAAGCGG AGCAAGAGGG ACCCCAGAAA CTGATCCGCA AAGTGTCTAC CTCGGGGCAG
    ATCCGGACCA AGACCAGTAT TAAAGAGGGA CAGCTATTGA AGCAAACCAG TTCTTTCCAA
    AGGTGGAAAA AGCGATACTT CAAACTTCGA GGCCGCACCC TTTACTATGC AAAGGACTCA
    AAGTCTCTGA TATTTGATGA AGTTGACCTC TCAGATGCTA GTGTAGCTGA AGCAAGCACG
    AAAAATGCTA ACAACAGCTT CACGATCATC ACTCCATTCA GAAGGCTAAT GCTGTGTGCT
    GAGAACAGAA AGGAGATGGA GGATTGGATC AGCTCACTGA AGTCTGTACA GACCAGAGAA
    CCCTACGAGG TGGCCCAGTT TAATGTGGAA CATTTCTCAG GGATGCACAA CTGGTACGCC
    TGCTCCCACG CCCGACCCAC CTTCTGTAAC GTGTGCAGAG AGAGTCTTTC TGGAGTCACC
    TCCCATGGCC TGTCCTGCGA AGTGTGTAAA TTCAAGGCTC ACAAAAGATG TGCAGTGAGA
    GCAACAAATA ACTGTAAATG GACTACCCTG GCCTCCATCG GGAAGGACAT TATAGAAGAT
    GAAGATGGCG TCGCGATGCC TCACCAGTGG CTTGAGGGCA ACCTGCCTGT AAGTGCCAAG
    TGTGCTGTCT GCGACAAAAC ATGTGGCAGT GTTCTCCGTC TACAGGATTG GAAATGCCTT
    TGGTGTAAGA CAATGGTACA CACTGCCTGC AAAGATTTAT ACCATCCAAT ATGTCCACTT
    GGTCAATGTA AAGTATCTAT CATACCTCCA ATTGCACTAA ACAGCACCGA TTCCGATGGT
    TTCTGTAGAG CAACATTTTC GTTCTGTGTT AGTCCTCTAT TGGTTTTTGT CAATTCTAAG
    AGTGGAGATA ATCAGGGAGT AAAGTTCCTC CGTCGCTTTA AACAGTTGCT AAATCCGGCT
    CAGGTGTTTG ATTTAATGAA TGGAGGTCCT CATTTAGGTT TAAGATTATT TCAGAAGTTT
    GACAATTTCC GGATTCTTGT TTGTGGAGGC GATGGAAGTG TAGGTTGGGT TTTGTCAGAA
    ATCGATAAGC TCAACTTGAA TAAACAGTGT CAGCTGGGAG TGTTGCCTTT GGGTACAGGA
    AATGACCTTG CCCGAGTTCT TGGCTGGGGA GGTTCATATG ACGATGACAC CCAACTTCCT
    CAGATCCTAG AGAAACTGGA ACGAGCCAGT ACCAAAATGT TGGACAGGTG GAGTATAATG
    ACATATGAAC TCAAATTGCC ACCAAAAGCT TCCCTACTTC CAGGACCTCC AGAAGCATCT
    GAAGAATTTT ATATGACGAT TTATGAAGAC TCAGTTGCAA CGCATCTTAC AAAAATCCTC
    AATTCTGATG AACATGCAGT GGTCATATCT TCTGCCAAGA CGCTATGTGA AACTGTAAAG
    GACTTCGTTG CCAAAGTAGA AAAGACGTAT GACAAAACCT TGGAAAATGC CGTTGTAGCT
    GATGCCGTGG CCAGTAAATG TTCAGTCCTA AACGAGAAGC TCGAACAACT GCTGCAGGCT
    TTGCACACAG ATTCCCAGGC TGCGCCTGTT CTCCCTGGCC TCAGCCCTCT CATTGTGGAA
    GAAGATGCTG TGGAATCGTC CAGTGAAGAG TCCCTGGGTG AAAGCAAAGA GCAGCTTGGG
    GATGACGTTA CAAAACCTTC CTCCCAGAAA GCCGTCAAAC CAAGGGAAAT CATGTTGCGG
    GCAAATAGTT TAAAGAAAGC AGTGAGGCAA GTCATTGAGG AAGCCGGAAA AGTTATGGAT
    GACCCGACAG TTCACCCCTG TGAACCAGCT AATCAGTCCT CTGATTATGA CAGCACAGAA
    ACAGATGAAT CTAAGGAGGA AGCTAAAGAT GATGGTGCCA AAGAATCAAT AACTGTTAAA
    ACTGCACCTC GGTCTCCAGA TGCCCGGGCA AGTTATGGCC ATTCCCAAAC TGATTCTGTC
    CCTGGTCCAG CTGTGGCAGC CAGCAAAGAA AACCTCCCTG TGCTCAATAC CAGAATAATC
    TGCCCAGGTT TAAGAGCAGG ACTGGCTGCC TCAATTGCTG GGAGTTCGAT TATCAACAAA
    ATGTTACTGG CAAACATTGA TCCTTTTGGT GCCACGCCGT TTATTGACCC GGATCTAGAT
    TCCGTAGATG GATATTCAGA AAAATGTGTC ATGAACAATT ACTTTGGGAT TGGATTAGAT
    GCAAAAATTT CATTAGAATT TAATAATAAA AGAGAGGAGC ACCCTGAAAA ATGCAGGAGC
    CGAACTAAAA ACTTGATGTG GTATGGAGTC CTTGGAACCC GGGAGTTATT ACAGAGATCG
    TACAAGAATT TAGAACAAAG GGTTCAACTT GAGTGTGATG GGCAGTATAT TCCTCTTCCC
    AGCTTGCAAG GCATAGCCGT GTTGAACATT CCCAGCTATG CTGGAGGCAC TAACTTTTGG
    GGTGGAACTA AAGAGGATGA TATATTTGCT GCACCATCCT TTGATGACAA GATCCTGGAA
    GTTGTAGCAA TATTTGATAG CATGCAAATG GCAGTTTCAA GGGTCATTAA ACTGCAGCAT
    CATCGAATAG CCCAGTGCCG TACAGTGAAA ATCACTATAT TTGGTGACGA AGGAGTCCCA
    GTGCAAGTGG ATGGTGAAGC GTGGGTTCAG CCTCCAGGGA TTATCAAAAT TGTGCACAAA
    AACAGAGCAC AAATGCTAAC AAGGGACAGA GCCTTTGAGA GCACTCTGAA ATCTTGGGAA
    GATAAGCAGA AGTGTGATTC TGGTAAACCA GTTCTCCGAA CCCATTTGTA CATCCATCAC
    GCCATTGACT TGGCAACAGA AGAGGTGTCG CAGATGCAGC TATGCTCCCA GGCTGCAGAG
    GAGCTCATTA CTAGGATATG TGACGCAGCC ACAATTCACT GTCTTTTGGA GCAAGAACTG
    GCCCATGCTG TGAATGCCTG CTCCCATGCC CTGAATAAAG CCAACCCAAG GTGCCCGGAG
    AGTCTTACAA GAGACACTGC CACTGAAATA GCCATCAATG TGAAGGCGCT GTATAATGAA
    ACAGAATCTT TGCTAGTTGG CAGGGTTCCT TTGCAGCTGG AATCGCCACA TGAAGAGCGA
    GTATCCAATG CCTTACACTC TGTGGAGGTG GAATTACAGA AACTGACAGA GATTCCTTGG
    CTTTATTATA TCTTACACCC AAATGAGGAT GAGGAACCTC CTATGGATTG CACCAAAAGG
    AACAACAGAA GCACCGTATT TCGAATAGTG CCAAAGTTTA AAAAGGAAAA GGTTCAGAAG
    CAGAAGACAA GTTCACAGCC TGGATCTGGG GATACCGAAA GTGGGTCATG TGAAGCGAAT
    TCTCCAGGGA ATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu28739
    Clone ID Related Accession (Same CDS sequence) NM_152910.5 , NM_152910.6 , NM_001204504.2
    Accession Version NM_152910.6 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3495bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-29
    Organism Homo sapiens(human)
    Product diacylglycerol kinase eta isoform 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL157932.21, DR002010.1, AB078967.1, AK302631.1, BC043292.2, AL136527.9 and BM978856.1. On Jun 2, 2019 this sequence version replaced NM_152910.5. Transcript Variant: This variant (1) encodes isoform 1. Variants 1 and 3 encode the same isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.216359.1, AB078967.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2142348 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGCAGGGG CCGGAGGCCA GCACCACCCT CCGGGCGCCG CTGGAGGAGC GGCCGCCGGA 
    GCCGGCGCCG CGGTCACCTC CGCCGCTGCC TCGGCGGGGC CGGGAGAGGA TTCGTCTGAC
    AGCGAAGCGG AGCAAGAGGG ACCCCAGAAA CTGATCCGCA AAGTGTCTAC CTCGGGGCAG
    ATCCGGACCA AGACCAGTAT TAAAGAGGGA CAGCTATTGA AGCAAACCAG TTCTTTCCAA
    AGGTGGAAAA AGCGATACTT CAAACTTCGA GGCCGCACCC TTTACTATGC AAAGGACTCA
    AAGTCTCTGA TATTTGATGA AGTTGACCTC TCAGATGCTA GTGTAGCTGA AGCAAGCACG
    AAAAATGCTA ACAACAGCTT CACGATCATC ACTCCATTCA GAAGGCTAAT GCTGTGTGCT
    GAGAACAGAA AGGAGATGGA GGATTGGATC AGCTCACTGA AGTCTGTACA GACCAGAGAA
    CCCTACGAGG TGGCCCAGTT TAATGTGGAA CATTTCTCAG GGATGCACAA CTGGTACGCC
    TGCTCCCACG CCCGACCCAC CTTCTGTAAC GTGTGCAGAG AGAGTCTTTC TGGAGTCACC
    TCCCATGGCC TGTCCTGCGA AGTGTGTAAA TTCAAGGCTC ACAAAAGATG TGCAGTGAGA
    GCAACAAATA ACTGTAAATG GACTACCCTG GCCTCCATCG GGAAGGACAT TATAGAAGAT
    GAAGATGGCG TCGCGATGCC TCACCAGTGG CTTGAGGGCA ACCTGCCTGT AAGTGCCAAG
    TGTGCTGTCT GCGACAAAAC ATGTGGCAGT GTTCTCCGTC TACAGGATTG GAAATGCCTT
    TGGTGTAAGA CAATGGTACA CACTGCCTGC AAAGATTTAT ACCATCCAAT ATGTCCACTT
    GGTCAATGTA AAGTATCTAT CATACCTCCA ATTGCACTAA ACAGCACCGA TTCCGATGGT
    TTCTGTAGAG CAACATTTTC GTTCTGTGTT AGTCCTCTAT TGGTTTTTGT CAATTCTAAG
    AGTGGAGATA ATCAGGGAGT AAAGTTCCTC CGTCGCTTTA AACAGTTGCT AAATCCGGCT
    CAGGTGTTTG ATTTAATGAA TGGAGGTCCT CATTTAGGTT TAAGATTATT TCAGAAGTTT
    GACAATTTCC GGATTCTTGT TTGTGGAGGC GATGGAAGTG TAGGTTGGGT TTTGTCAGAA
    ATCGATAAGC TCAACTTGAA TAAACAGTGT CAGCTGGGAG TGTTGCCTTT GGGTACAGGA
    AATGACCTTG CCCGAGTTCT TGGCTGGGGA GGTTCATATG ACGATGACAC CCAACTTCCT
    CAGATCCTAG AGAAACTGGA ACGAGCCAGT ACCAAAATGT TGGACAGGTG GAGTATAATG
    ACATATGAAC TCAAATTGCC ACCAAAAGCT TCCCTACTTC CAGGACCTCC AGAAGCATCT
    GAAGAATTTT ATATGACGAT TTATGAAGAC TCAGTTGCAA CGCATCTTAC AAAAATCCTC
    AATTCTGATG AACATGCAGT GGTCATATCT TCTGCCAAGA CGCTATGTGA AACTGTAAAG
    GACTTCGTTG CCAAAGTAGA AAAGACGTAT GACAAAACCT TGGAAAATGC CGTTGTAGCT
    GATGCCGTGG CCAGTAAATG TTCAGTCCTA AACGAGAAGC TCGAACAACT GCTGCAGGCT
    TTGCACACAG ATTCCCAGGC TGCGCCTGTT CTCCCTGGCC TCAGCCCTCT CATTGTGGAA
    GAAGATGCTG TGGAATCGTC CAGTGAAGAG TCCCTGGGTG AAAGCAAAGA GCAGCTTGGG
    GATGACGTTA CAAAACCTTC CTCCCAGAAA GCCGTCAAAC CAAGGGAAAT CATGTTGCGG
    GCAAATAGTT TAAAGAAAGC AGTGAGGCAA GTCATTGAGG AAGCCGGAAA AGTTATGGAT
    GACCCGACAG TTCACCCCTG TGAACCAGCT AATCAGTCCT CTGATTATGA CAGCACAGAA
    ACAGATGAAT CTAAGGAGGA AGCTAAAGAT GATGGTGCCA AAGAATCAAT AACTGTTAAA
    ACTGCACCTC GGTCTCCAGA TGCCCGGGCA AGTTATGGCC ATTCCCAAAC TGATTCTGTC
    CCTGGTCCAG CTGTGGCAGC CAGCAAAGAA AACCTCCCTG TGCTCAATAC CAGAATAATC
    TGCCCAGGTT TAAGAGCAGG ACTGGCTGCC TCAATTGCTG GGAGTTCGAT TATCAACAAA
    ATGTTACTGG CAAACATTGA TCCTTTTGGT GCCACGCCGT TTATTGACCC GGATCTAGAT
    TCCGTAGATG GATATTCAGA AAAATGTGTC ATGAACAATT ACTTTGGGAT TGGATTAGAT
    GCAAAAATTT CATTAGAATT TAATAATAAA AGAGAGGAGC ACCCTGAAAA ATGCAGGAGC
    CGAACTAAAA ACTTGATGTG GTATGGAGTC CTTGGAACCC GGGAGTTATT ACAGAGATCG
    TACAAGAATT TAGAACAAAG GGTTCAACTT GAGTGTGATG GGCAGTATAT TCCTCTTCCC
    AGCTTGCAAG GCATAGCCGT GTTGAACATT CCCAGCTATG CTGGAGGCAC TAACTTTTGG
    GGTGGAACTA AAGAGGATGA TATATTTGCT GCACCATCCT TTGATGACAA GATCCTGGAA
    GTTGTAGCAA TATTTGATAG CATGCAAATG GCAGTTTCAA GGGTCATTAA ACTGCAGCAT
    CATCGAATAG CCCAGTGCCG TACAGTGAAA ATCACTATAT TTGGTGACGA AGGAGTCCCA
    GTGCAAGTGG ATGGTGAAGC GTGGGTTCAG CCTCCAGGGA TTATCAAAAT TGTGCACAAA
    AACAGAGCAC AAATGCTAAC AAGGGACAGA GCCTTTGAGA GCACTCTGAA ATCTTGGGAA
    GATAAGCAGA AGTGTGATTC TGGTAAACCA GTTCTCCGAA CCCATTTGTA CATCCATCAC
    GCCATTGACT TGGCAACAGA AGAGGTGTCG CAGATGCAGC TATGCTCCCA GGCTGCAGAG
    GAGCTCATTA CTAGGATATG TGACGCAGCC ACAATTCACT GTCTTTTGGA GCAAGAACTG
    GCCCATGCTG TGAATGCCTG CTCCCATGCC CTGAATAAAG CCAACCCAAG GTGCCCGGAG
    AGTCTTACAA GAGACACTGC CACTGAAATA GCCATCAATG TGAAGGCGCT GTATAATGAA
    ACAGAATCTT TGCTAGTTGG CAGGGTTCCT TTGCAGCTGG AATCGCCACA TGAAGAGCGA
    GTATCCAATG CCTTACACTC TGTGGAGGTG GAATTACAGA AACTGACAGA GATTCCTTGG
    CTTTATTATA TCTTACACCC AAATGAGGAT GAGGAACCTC CTATGGATTG CACCAAAAGG
    AACAACAGAA GCACCGTATT TCGAATAGTG CCAAAGTTTA AAAAGGAAAA GGTTCAGAAG
    CAGAAGACAA GTTCACAGCC TGGATCTGGG GATACCGAAA GTGGGTCATG TGAAGCGAAT
    TCTCCAGGGA ATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_690874.2
    CDS127..3621
    Translation

    Target ORF information:

    RefSeq Version NM_152910.6
    Organism Homo sapiens(human)
    Definition Homo sapiens diacylglycerol kinase eta (DGKH), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_152910.6

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGCAGGGG CCGGAGGCCA GCACCACCCT CCGGGCGCCG CTGGAGGAGC GGCCGCCGGA 
    GCCGGCGCCG CGGTCACCTC CGCCGCTGCC TCGGCGGGGC CGGGAGAGGA TTCGTCTGAC
    AGCGAAGCGG AGCAAGAGGG ACCCCAGAAA CTGATCCGCA AAGTGTCTAC CTCGGGGCAG
    ATCCGGACCA AGACCAGTAT TAAAGAGGGA CAGCTATTGA AGCAAACCAG TTCTTTCCAA
    AGGTGGAAAA AGCGATACTT CAAACTTCGA GGCCGCACCC TTTACTATGC AAAGGACTCA
    AAGTCTCTGA TATTTGATGA AGTTGACCTC TCAGATGCTA GTGTAGCTGA AGCAAGCACG
    AAAAATGCTA ACAACAGCTT CACGATCATC ACTCCATTCA GAAGGCTAAT GCTGTGTGCT
    GAGAACAGAA AGGAGATGGA GGATTGGATC AGCTCACTGA AGTCTGTACA GACCAGAGAA
    CCCTACGAGG TGGCCCAGTT TAATGTGGAA CATTTCTCAG GGATGCACAA CTGGTACGCC
    TGCTCCCACG CCCGACCCAC CTTCTGTAAC GTGTGCAGAG AGAGTCTTTC TGGAGTCACC
    TCCCATGGCC TGTCCTGCGA AGTGTGTAAA TTCAAGGCTC ACAAAAGATG TGCAGTGAGA
    GCAACAAATA ACTGTAAATG GACTACCCTG GCCTCCATCG GGAAGGACAT TATAGAAGAT
    GAAGATGGCG TCGCGATGCC TCACCAGTGG CTTGAGGGCA ACCTGCCTGT AAGTGCCAAG
    TGTGCTGTCT GCGACAAAAC ATGTGGCAGT GTTCTCCGTC TACAGGATTG GAAATGCCTT
    TGGTGTAAGA CAATGGTACA CACTGCCTGC AAAGATTTAT ACCATCCAAT ATGTCCACTT
    GGTCAATGTA AAGTATCTAT CATACCTCCA ATTGCACTAA ACAGCACCGA TTCCGATGGT
    TTCTGTAGAG CAACATTTTC GTTCTGTGTT AGTCCTCTAT TGGTTTTTGT CAATTCTAAG
    AGTGGAGATA ATCAGGGAGT AAAGTTCCTC CGTCGCTTTA AACAGTTGCT AAATCCGGCT
    CAGGTGTTTG ATTTAATGAA TGGAGGTCCT CATTTAGGTT TAAGATTATT TCAGAAGTTT
    GACAATTTCC GGATTCTTGT TTGTGGAGGC GATGGAAGTG TAGGTTGGGT TTTGTCAGAA
    ATCGATAAGC TCAACTTGAA TAAACAGTGT CAGCTGGGAG TGTTGCCTTT GGGTACAGGA
    AATGACCTTG CCCGAGTTCT TGGCTGGGGA GGTTCATATG ACGATGACAC CCAACTTCCT
    CAGATCCTAG AGAAACTGGA ACGAGCCAGT ACCAAAATGT TGGACAGGTG GAGTATAATG
    ACATATGAAC TCAAATTGCC ACCAAAAGCT TCCCTACTTC CAGGACCTCC AGAAGCATCT
    GAAGAATTTT ATATGACGAT TTATGAAGAC TCAGTTGCAA CGCATCTTAC AAAAATCCTC
    AATTCTGATG AACATGCAGT GGTCATATCT TCTGCCAAGA CGCTATGTGA AACTGTAAAG
    GACTTCGTTG CCAAAGTAGA AAAGACGTAT GACAAAACCT TGGAAAATGC CGTTGTAGCT
    GATGCCGTGG CCAGTAAATG TTCAGTCCTA AACGAGAAGC TCGAACAACT GCTGCAGGCT
    TTGCACACAG ATTCCCAGGC TGCGCCTGTT CTCCCTGGCC TCAGCCCTCT CATTGTGGAA
    GAAGATGCTG TGGAATCGTC CAGTGAAGAG TCCCTGGGTG AAAGCAAAGA GCAGCTTGGG
    GATGACGTTA CAAAACCTTC CTCCCAGAAA GCCGTCAAAC CAAGGGAAAT CATGTTGCGG
    GCAAATAGTT TAAAGAAAGC AGTGAGGCAA GTCATTGAGG AAGCCGGAAA AGTTATGGAT
    GACCCGACAG TTCACCCCTG TGAACCAGCT AATCAGTCCT CTGATTATGA CAGCACAGAA
    ACAGATGAAT CTAAGGAGGA AGCTAAAGAT GATGGTGCCA AAGAATCAAT AACTGTTAAA
    ACTGCACCTC GGTCTCCAGA TGCCCGGGCA AGTTATGGCC ATTCCCAAAC TGATTCTGTC
    CCTGGTCCAG CTGTGGCAGC CAGCAAAGAA AACCTCCCTG TGCTCAATAC CAGAATAATC
    TGCCCAGGTT TAAGAGCAGG ACTGGCTGCC TCAATTGCTG GGAGTTCGAT TATCAACAAA
    ATGTTACTGG CAAACATTGA TCCTTTTGGT GCCACGCCGT TTATTGACCC GGATCTAGAT
    TCCGTAGATG GATATTCAGA AAAATGTGTC ATGAACAATT ACTTTGGGAT TGGATTAGAT
    GCAAAAATTT CATTAGAATT TAATAATAAA AGAGAGGAGC ACCCTGAAAA ATGCAGGAGC
    CGAACTAAAA ACTTGATGTG GTATGGAGTC CTTGGAACCC GGGAGTTATT ACAGAGATCG
    TACAAGAATT TAGAACAAAG GGTTCAACTT GAGTGTGATG GGCAGTATAT TCCTCTTCCC
    AGCTTGCAAG GCATAGCCGT GTTGAACATT CCCAGCTATG CTGGAGGCAC TAACTTTTGG
    GGTGGAACTA AAGAGGATGA TATATTTGCT GCACCATCCT TTGATGACAA GATCCTGGAA
    GTTGTAGCAA TATTTGATAG CATGCAAATG GCAGTTTCAA GGGTCATTAA ACTGCAGCAT
    CATCGAATAG CCCAGTGCCG TACAGTGAAA ATCACTATAT TTGGTGACGA AGGAGTCCCA
    GTGCAAGTGG ATGGTGAAGC GTGGGTTCAG CCTCCAGGGA TTATCAAAAT TGTGCACAAA
    AACAGAGCAC AAATGCTAAC AAGGGACAGA GCCTTTGAGA GCACTCTGAA ATCTTGGGAA
    GATAAGCAGA AGTGTGATTC TGGTAAACCA GTTCTCCGAA CCCATTTGTA CATCCATCAC
    GCCATTGACT TGGCAACAGA AGAGGTGTCG CAGATGCAGC TATGCTCCCA GGCTGCAGAG
    GAGCTCATTA CTAGGATATG TGACGCAGCC ACAATTCACT GTCTTTTGGA GCAAGAACTG
    GCCCATGCTG TGAATGCCTG CTCCCATGCC CTGAATAAAG CCAACCCAAG GTGCCCGGAG
    AGTCTTACAA GAGACACTGC CACTGAAATA GCCATCAATG TGAAGGCGCT GTATAATGAA
    ACAGAATCTT TGCTAGTTGG CAGGGTTCCT TTGCAGCTGG AATCGCCACA TGAAGAGCGA
    GTATCCAATG CCTTACACTC TGTGGAGGTG GAATTACAGA AACTGACAGA GATTCCTTGG
    CTTTATTATA TCTTACACCC AAATGAGGAT GAGGAACCTC CTATGGATTG CACCAAAAGG
    AACAACAGAA GCACCGTATT TCGAATAGTG CCAAAGTTTA AAAAGGAAAA GGTTCAGAAG
    CAGAAGACAA GTTCACAGCC TGGATCTGGG GATACCGAAA GTGGGTCATG TGAAGCGAAT
    TCTCCAGGGA ATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu28739
    Clone ID Related Accession (Same CDS sequence) NM_152910.5 , NM_152910.6 , NM_001204504.2
    Accession Version NM_001204504.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3495bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-24
    Organism Homo sapiens(human)
    Product diacylglycerol kinase eta isoform 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK289546.1, AB078968.1, AK302727.1, BC043292.2, AL136527.9 and BM978856.1. On Jul 2, 2014 this sequence version replaced NM_001204504.1. Transcript Variant: This variant (3) differs in the 5' UTR, compared to variant 1. Variants 1 and 3 encode the same isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK289546.1 [ECO:0000332] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGCAGGGG CCGGAGGCCA GCACCACCCT CCGGGCGCCG CTGGAGGAGC GGCCGCCGGA 
    GCCGGCGCCG CGGTCACCTC CGCCGCTGCC TCGGCGGGGC CGGGAGAGGA TTCGTCTGAC
    AGCGAAGCGG AGCAAGAGGG ACCCCAGAAA CTGATCCGCA AAGTGTCTAC CTCGGGGCAG
    ATCCGGACCA AGACCAGTAT TAAAGAGGGA CAGCTATTGA AGCAAACCAG TTCTTTCCAA
    AGGTGGAAAA AGCGATACTT CAAACTTCGA GGCCGCACCC TTTACTATGC AAAGGACTCA
    AAGTCTCTGA TATTTGATGA AGTTGACCTC TCAGATGCTA GTGTAGCTGA AGCAAGCACG
    AAAAATGCTA ACAACAGCTT CACGATCATC ACTCCATTCA GAAGGCTAAT GCTGTGTGCT
    GAGAACAGAA AGGAGATGGA GGATTGGATC AGCTCACTGA AGTCTGTACA GACCAGAGAA
    CCCTACGAGG TGGCCCAGTT TAATGTGGAA CATTTCTCAG GGATGCACAA CTGGTACGCC
    TGCTCCCACG CCCGACCCAC CTTCTGTAAC GTGTGCAGAG AGAGTCTTTC TGGAGTCACC
    TCCCATGGCC TGTCCTGCGA AGTGTGTAAA TTCAAGGCTC ACAAAAGATG TGCAGTGAGA
    GCAACAAATA ACTGTAAATG GACTACCCTG GCCTCCATCG GGAAGGACAT TATAGAAGAT
    GAAGATGGCG TCGCGATGCC TCACCAGTGG CTTGAGGGCA ACCTGCCTGT AAGTGCCAAG
    TGTGCTGTCT GCGACAAAAC ATGTGGCAGT GTTCTCCGTC TACAGGATTG GAAATGCCTT
    TGGTGTAAGA CAATGGTACA CACTGCCTGC AAAGATTTAT ACCATCCAAT ATGTCCACTT
    GGTCAATGTA AAGTATCTAT CATACCTCCA ATTGCACTAA ACAGCACCGA TTCCGATGGT
    TTCTGTAGAG CAACATTTTC GTTCTGTGTT AGTCCTCTAT TGGTTTTTGT CAATTCTAAG
    AGTGGAGATA ATCAGGGAGT AAAGTTCCTC CGTCGCTTTA AACAGTTGCT AAATCCGGCT
    CAGGTGTTTG ATTTAATGAA TGGAGGTCCT CATTTAGGTT TAAGATTATT TCAGAAGTTT
    GACAATTTCC GGATTCTTGT TTGTGGAGGC GATGGAAGTG TAGGTTGGGT TTTGTCAGAA
    ATCGATAAGC TCAACTTGAA TAAACAGTGT CAGCTGGGAG TGTTGCCTTT GGGTACAGGA
    AATGACCTTG CCCGAGTTCT TGGCTGGGGA GGTTCATATG ACGATGACAC CCAACTTCCT
    CAGATCCTAG AGAAACTGGA ACGAGCCAGT ACCAAAATGT TGGACAGGTG GAGTATAATG
    ACATATGAAC TCAAATTGCC ACCAAAAGCT TCCCTACTTC CAGGACCTCC AGAAGCATCT
    GAAGAATTTT ATATGACGAT TTATGAAGAC TCAGTTGCAA CGCATCTTAC AAAAATCCTC
    AATTCTGATG AACATGCAGT GGTCATATCT TCTGCCAAGA CGCTATGTGA AACTGTAAAG
    GACTTCGTTG CCAAAGTAGA AAAGACGTAT GACAAAACCT TGGAAAATGC CGTTGTAGCT
    GATGCCGTGG CCAGTAAATG TTCAGTCCTA AACGAGAAGC TCGAACAACT GCTGCAGGCT
    TTGCACACAG ATTCCCAGGC TGCGCCTGTT CTCCCTGGCC TCAGCCCTCT CATTGTGGAA
    GAAGATGCTG TGGAATCGTC CAGTGAAGAG TCCCTGGGTG AAAGCAAAGA GCAGCTTGGG
    GATGACGTTA CAAAACCTTC CTCCCAGAAA GCCGTCAAAC CAAGGGAAAT CATGTTGCGG
    GCAAATAGTT TAAAGAAAGC AGTGAGGCAA GTCATTGAGG AAGCCGGAAA AGTTATGGAT
    GACCCGACAG TTCACCCCTG TGAACCAGCT AATCAGTCCT CTGATTATGA CAGCACAGAA
    ACAGATGAAT CTAAGGAGGA AGCTAAAGAT GATGGTGCCA AAGAATCAAT AACTGTTAAA
    ACTGCACCTC GGTCTCCAGA TGCCCGGGCA AGTTATGGCC ATTCCCAAAC TGATTCTGTC
    CCTGGTCCAG CTGTGGCAGC CAGCAAAGAA AACCTCCCTG TGCTCAATAC CAGAATAATC
    TGCCCAGGTT TAAGAGCAGG ACTGGCTGCC TCAATTGCTG GGAGTTCGAT TATCAACAAA
    ATGTTACTGG CAAACATTGA TCCTTTTGGT GCCACGCCGT TTATTGACCC GGATCTAGAT
    TCCGTAGATG GATATTCAGA AAAATGTGTC ATGAACAATT ACTTTGGGAT TGGATTAGAT
    GCAAAAATTT CATTAGAATT TAATAATAAA AGAGAGGAGC ACCCTGAAAA ATGCAGGAGC
    CGAACTAAAA ACTTGATGTG GTATGGAGTC CTTGGAACCC GGGAGTTATT ACAGAGATCG
    TACAAGAATT TAGAACAAAG GGTTCAACTT GAGTGTGATG GGCAGTATAT TCCTCTTCCC
    AGCTTGCAAG GCATAGCCGT GTTGAACATT CCCAGCTATG CTGGAGGCAC TAACTTTTGG
    GGTGGAACTA AAGAGGATGA TATATTTGCT GCACCATCCT TTGATGACAA GATCCTGGAA
    GTTGTAGCAA TATTTGATAG CATGCAAATG GCAGTTTCAA GGGTCATTAA ACTGCAGCAT
    CATCGAATAG CCCAGTGCCG TACAGTGAAA ATCACTATAT TTGGTGACGA AGGAGTCCCA
    GTGCAAGTGG ATGGTGAAGC GTGGGTTCAG CCTCCAGGGA TTATCAAAAT TGTGCACAAA
    AACAGAGCAC AAATGCTAAC AAGGGACAGA GCCTTTGAGA GCACTCTGAA ATCTTGGGAA
    GATAAGCAGA AGTGTGATTC TGGTAAACCA GTTCTCCGAA CCCATTTGTA CATCCATCAC
    GCCATTGACT TGGCAACAGA AGAGGTGTCG CAGATGCAGC TATGCTCCCA GGCTGCAGAG
    GAGCTCATTA CTAGGATATG TGACGCAGCC ACAATTCACT GTCTTTTGGA GCAAGAACTG
    GCCCATGCTG TGAATGCCTG CTCCCATGCC CTGAATAAAG CCAACCCAAG GTGCCCGGAG
    AGTCTTACAA GAGACACTGC CACTGAAATA GCCATCAATG TGAAGGCGCT GTATAATGAA
    ACAGAATCTT TGCTAGTTGG CAGGGTTCCT TTGCAGCTGG AATCGCCACA TGAAGAGCGA
    GTATCCAATG CCTTACACTC TGTGGAGGTG GAATTACAGA AACTGACAGA GATTCCTTGG
    CTTTATTATA TCTTACACCC AAATGAGGAT GAGGAACCTC CTATGGATTG CACCAAAAGG
    AACAACAGAA GCACCGTATT TCGAATAGTG CCAAAGTTTA AAAAGGAAAA GGTTCAGAAG
    CAGAAGACAA GTTCACAGCC TGGATCTGGG GATACCGAAA GTGGGTCATG TGAAGCGAAT
    TCTCCAGGGA ATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001191433.1
    CDS104..3598
    Translation

    Target ORF information:

    RefSeq Version NM_001204504.2
    Organism Homo sapiens(human)
    Definition Homo sapiens diacylglycerol kinase eta (DGKH), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001204504.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGCAGGGG CCGGAGGCCA GCACCACCCT CCGGGCGCCG CTGGAGGAGC GGCCGCCGGA 
    GCCGGCGCCG CGGTCACCTC CGCCGCTGCC TCGGCGGGGC CGGGAGAGGA TTCGTCTGAC
    AGCGAAGCGG AGCAAGAGGG ACCCCAGAAA CTGATCCGCA AAGTGTCTAC CTCGGGGCAG
    ATCCGGACCA AGACCAGTAT TAAAGAGGGA CAGCTATTGA AGCAAACCAG TTCTTTCCAA
    AGGTGGAAAA AGCGATACTT CAAACTTCGA GGCCGCACCC TTTACTATGC AAAGGACTCA
    AAGTCTCTGA TATTTGATGA AGTTGACCTC TCAGATGCTA GTGTAGCTGA AGCAAGCACG
    AAAAATGCTA ACAACAGCTT CACGATCATC ACTCCATTCA GAAGGCTAAT GCTGTGTGCT
    GAGAACAGAA AGGAGATGGA GGATTGGATC AGCTCACTGA AGTCTGTACA GACCAGAGAA
    CCCTACGAGG TGGCCCAGTT TAATGTGGAA CATTTCTCAG GGATGCACAA CTGGTACGCC
    TGCTCCCACG CCCGACCCAC CTTCTGTAAC GTGTGCAGAG AGAGTCTTTC TGGAGTCACC
    TCCCATGGCC TGTCCTGCGA AGTGTGTAAA TTCAAGGCTC ACAAAAGATG TGCAGTGAGA
    GCAACAAATA ACTGTAAATG GACTACCCTG GCCTCCATCG GGAAGGACAT TATAGAAGAT
    GAAGATGGCG TCGCGATGCC TCACCAGTGG CTTGAGGGCA ACCTGCCTGT AAGTGCCAAG
    TGTGCTGTCT GCGACAAAAC ATGTGGCAGT GTTCTCCGTC TACAGGATTG GAAATGCCTT
    TGGTGTAAGA CAATGGTACA CACTGCCTGC AAAGATTTAT ACCATCCAAT ATGTCCACTT
    GGTCAATGTA AAGTATCTAT CATACCTCCA ATTGCACTAA ACAGCACCGA TTCCGATGGT
    TTCTGTAGAG CAACATTTTC GTTCTGTGTT AGTCCTCTAT TGGTTTTTGT CAATTCTAAG
    AGTGGAGATA ATCAGGGAGT AAAGTTCCTC CGTCGCTTTA AACAGTTGCT AAATCCGGCT
    CAGGTGTTTG ATTTAATGAA TGGAGGTCCT CATTTAGGTT TAAGATTATT TCAGAAGTTT
    GACAATTTCC GGATTCTTGT TTGTGGAGGC GATGGAAGTG TAGGTTGGGT TTTGTCAGAA
    ATCGATAAGC TCAACTTGAA TAAACAGTGT CAGCTGGGAG TGTTGCCTTT GGGTACAGGA
    AATGACCTTG CCCGAGTTCT TGGCTGGGGA GGTTCATATG ACGATGACAC CCAACTTCCT
    CAGATCCTAG AGAAACTGGA ACGAGCCAGT ACCAAAATGT TGGACAGGTG GAGTATAATG
    ACATATGAAC TCAAATTGCC ACCAAAAGCT TCCCTACTTC CAGGACCTCC AGAAGCATCT
    GAAGAATTTT ATATGACGAT TTATGAAGAC TCAGTTGCAA CGCATCTTAC AAAAATCCTC
    AATTCTGATG AACATGCAGT GGTCATATCT TCTGCCAAGA CGCTATGTGA AACTGTAAAG
    GACTTCGTTG CCAAAGTAGA AAAGACGTAT GACAAAACCT TGGAAAATGC CGTTGTAGCT
    GATGCCGTGG CCAGTAAATG TTCAGTCCTA AACGAGAAGC TCGAACAACT GCTGCAGGCT
    TTGCACACAG ATTCCCAGGC TGCGCCTGTT CTCCCTGGCC TCAGCCCTCT CATTGTGGAA
    GAAGATGCTG TGGAATCGTC CAGTGAAGAG TCCCTGGGTG AAAGCAAAGA GCAGCTTGGG
    GATGACGTTA CAAAACCTTC CTCCCAGAAA GCCGTCAAAC CAAGGGAAAT CATGTTGCGG
    GCAAATAGTT TAAAGAAAGC AGTGAGGCAA GTCATTGAGG AAGCCGGAAA AGTTATGGAT
    GACCCGACAG TTCACCCCTG TGAACCAGCT AATCAGTCCT CTGATTATGA CAGCACAGAA
    ACAGATGAAT CTAAGGAGGA AGCTAAAGAT GATGGTGCCA AAGAATCAAT AACTGTTAAA
    ACTGCACCTC GGTCTCCAGA TGCCCGGGCA AGTTATGGCC ATTCCCAAAC TGATTCTGTC
    CCTGGTCCAG CTGTGGCAGC CAGCAAAGAA AACCTCCCTG TGCTCAATAC CAGAATAATC
    TGCCCAGGTT TAAGAGCAGG ACTGGCTGCC TCAATTGCTG GGAGTTCGAT TATCAACAAA
    ATGTTACTGG CAAACATTGA TCCTTTTGGT GCCACGCCGT TTATTGACCC GGATCTAGAT
    TCCGTAGATG GATATTCAGA AAAATGTGTC ATGAACAATT ACTTTGGGAT TGGATTAGAT
    GCAAAAATTT CATTAGAATT TAATAATAAA AGAGAGGAGC ACCCTGAAAA ATGCAGGAGC
    CGAACTAAAA ACTTGATGTG GTATGGAGTC CTTGGAACCC GGGAGTTATT ACAGAGATCG
    TACAAGAATT TAGAACAAAG GGTTCAACTT GAGTGTGATG GGCAGTATAT TCCTCTTCCC
    AGCTTGCAAG GCATAGCCGT GTTGAACATT CCCAGCTATG CTGGAGGCAC TAACTTTTGG
    GGTGGAACTA AAGAGGATGA TATATTTGCT GCACCATCCT TTGATGACAA GATCCTGGAA
    GTTGTAGCAA TATTTGATAG CATGCAAATG GCAGTTTCAA GGGTCATTAA ACTGCAGCAT
    CATCGAATAG CCCAGTGCCG TACAGTGAAA ATCACTATAT TTGGTGACGA AGGAGTCCCA
    GTGCAAGTGG ATGGTGAAGC GTGGGTTCAG CCTCCAGGGA TTATCAAAAT TGTGCACAAA
    AACAGAGCAC AAATGCTAAC AAGGGACAGA GCCTTTGAGA GCACTCTGAA ATCTTGGGAA
    GATAAGCAGA AGTGTGATTC TGGTAAACCA GTTCTCCGAA CCCATTTGTA CATCCATCAC
    GCCATTGACT TGGCAACAGA AGAGGTGTCG CAGATGCAGC TATGCTCCCA GGCTGCAGAG
    GAGCTCATTA CTAGGATATG TGACGCAGCC ACAATTCACT GTCTTTTGGA GCAAGAACTG
    GCCCATGCTG TGAATGCCTG CTCCCATGCC CTGAATAAAG CCAACCCAAG GTGCCCGGAG
    AGTCTTACAA GAGACACTGC CACTGAAATA GCCATCAATG TGAAGGCGCT GTATAATGAA
    ACAGAATCTT TGCTAGTTGG CAGGGTTCCT TTGCAGCTGG AATCGCCACA TGAAGAGCGA
    GTATCCAATG CCTTACACTC TGTGGAGGTG GAATTACAGA AACTGACAGA GATTCCTTGG
    CTTTATTATA TCTTACACCC AAATGAGGAT GAGGAACCTC CTATGGATTG CACCAAAAGG
    AACAACAGAA GCACCGTATT TCGAATAGTG CCAAAGTTTA AAAAGGAAAA GGTTCAGAAG
    CAGAAGACAA GTTCACAGCC TGGATCTGGG GATACCGAAA GTGGGTCATG TGAAGCGAAT
    TCTCCAGGGA ATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu28741
    Clone ID Related Accession (Same CDS sequence) NM_001204506.2 , NM_001204506.3
    Accession Version NM_001204506.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3255bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-01-26
    Organism Homo sapiens(human)
    Product diacylglycerol kinase eta isoform 4
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK302727.1, AB078967.1, BC043292.2, AL136527.9 and BM978856.1. On Jul 2, 2014 this sequence version replaced NM_001204506.1. Transcript Variant: This variant (5) differs in the 5' UTR, lacks a portion of the 5' coding region, and contains an alternate exon in the 3' coding region, which results in a frameshift, compared to variant 1. It initiates translation at a downstream in-frame start codon. The encoded isoform (4) has a shorter N-terminus and a distinct C-terminus, and is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK302727.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2148093 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGCTGTGTG CTGAGAACAG AAAGGAGATG GAGGATTGGA TCAGCTCACT GAAGTCTGTA 
    CAGACCAGAG AACCCTACGA GGTGGCCCAG TTTAATGTGG AACATTTCTC AGGGATGCAC
    AACTGGTACG CCTGCTCCCA CGCCCGACCC ACCTTCTGTA ACGTGTGCAG AGAGAGTCTT
    TCTGGAGTCA CCTCCCATGG CCTGTCCTGC GAAGTGTGTA AATTCAAGGC TCACAAAAGA
    TGTGCAGTGA GAGCAACAAA TAACTGTAAA TGGACTACCC TGGCCTCCAT CGGGAAGGAC
    ATTATAGAAG ATGAAGATGG CGTCGCGATG CCTCACCAGT GGCTTGAGGG CAACCTGCCT
    GTAAGTGCCA AGTGTGCTGT CTGCGACAAA ACATGTGGCA GTGTTCTCCG TCTACAGGAT
    TGGAAATGCC TTTGGTGTAA GACAATGGTA CACACTGCCT GCAAAGATTT ATACCATCCA
    ATATGTCCAC TTGGTCAATG TAAAGTATCT ATCATACCTC CAATTGCACT AAACAGCACC
    GATTCCGATG GTTTCTGTAG AGCAACATTT TCGTTCTGTG TTAGTCCTCT ATTGGTTTTT
    GTCAATTCTA AGAGTGGAGA TAATCAGGGA GTAAAGTTCC TCCGTCGCTT TAAACAGTTG
    CTAAATCCGG CTCAGGTGTT TGATTTAATG AATGGAGGTC CTCATTTAGG TTTAAGATTA
    TTTCAGAAGT TTGACAATTT CCGGATTCTT GTTTGTGGAG GCGATGGAAG TGTAGGTTGG
    GTTTTGTCAG AAATCGATAA GCTCAACTTG AATAAACAGT GTCAGCTGGG AGTGTTGCCT
    TTGGGTACAG GAAATGACCT TGCCCGAGTT CTTGGCTGGG GAGGTTCATA TGACGATGAC
    ACCCAACTTC CTCAGATCCT AGAGAAACTG GAACGAGCCA GTACCAAAAT GTTGGACAGG
    TGGAGTATAA TGACATATGA ACTCAAATTG CCACCAAAAG CTTCCCTACT TCCAGGACCT
    CCAGAAGCAT CTGAAGAATT TTATATGACG ATTTATGAAG ACTCAGTTGC AACGCATCTT
    ACAAAAATCC TCAATTCTGA TGAACATGCA GTGGTCATAT CTTCTGCCAA GACGCTATGT
    GAAACTGTAA AGGACTTCGT TGCCAAAGTA GAAAAGACGT ATGACAAAAC CTTGGAAAAT
    GCCGTTGTAG CTGATGCCGT GGCCAGTAAA TGTTCAGTCC TAAACGAGAA GCTCGAACAA
    CTGCTGCAGG CTTTGCACAC AGATTCCCAG GCTGCGCCTG TTCTCCCTGG CCTCAGCCCT
    CTCATTGTGG AAGAAGATGC TGTGGAATCG TCCAGTGAAG AGTCCCTGGG TGAAAGCAAA
    GAGCAGCTTG GGGATGACGT TACAAAACCT TCCTCCCAGA AAGCCGTCAA ACCAAGGGAA
    ATCATGTTGC GGGCAAATAG TTTAAAGAAA GCAGTGAGGC AAGTCATTGA GGAAGCCGGA
    AAAGTTATGG ATGACCCGAC AGTTCACCCC TGTGAACCAG CTAATCAGTC CTCTGATTAT
    GACAGCACAG AAACAGATGA ATCTAAGGAG GAAGCTAAAG ATGATGGTGC CAAAGAATCA
    ATAACTGTTA AAACTGCACC TCGGTCTCCA GATGCCCGGG CAAGTTATGG CCATTCCCAA
    ACTGATTCTG TCCCTGGTCC AGCTGTGGCA GCCAGCAAAG AAAACCTCCC TGTGCTCAAT
    ACCAGAATAA TCTGCCCAGG TTTAAGAGCA GGACTGGCTG CCTCAATTGC TGGGAGTTCG
    ATTATCAACA AAATGTTACT GGCAAACATT GATCCTTTTG GTGCCACGCC GTTTATTGAC
    CCGGATCTAG ATTCCGTAGA TGGATATTCA GAAAAATGTG TCATGAACAA TTACTTTGGG
    ATTGGATTAG ATGCAAAAAT TTCATTAGAA TTTAATAATA AAAGAGAGGA GCACCCTGAA
    AAATGCAGGA GCCGAACTAA AAACTTGATG TGGTATGGAG TCCTTGGAAC CCGGGAGTTA
    TTACAGAGAT CGTACAAGAA TTTAGAACAA AGGGTTCAAC TTGAGTGTGA TGGGCAGTAT
    ATTCCTCTTC CCAGCTTGCA AGGCATAGCC GTGTTGAACA TTCCCAGCTA TGCTGGAGGC
    ACTAACTTTT GGGGTGGAAC TAAAGAGGAT GATATATTTG CTGCACCATC CTTTGATGAC
    AAGATCCTGG AAGTTGTAGC AATATTTGAT AGCATGCAAA TGGCAGTTTC AAGGGTCATT
    AAACTGCAGC ATCATCGAAT AGCCCAGTGC CGTACAGTGA AAATCACTAT ATTTGGTGAC
    GAAGGAGTCC CAGTGCAAGT GGATGGTGAA GCGTGGGTTC AGCCTCCAGG GATTATCAAA
    ATTGTGCACA AAAACAGAGC ACAAATGCTA ACAAGGGACA GAGCCTTTGA GAGCACTCTG
    AAATCTTGGG AAGATAAGCA GAAGTGTGAT TCTGGTAAAC CAGTTCTCCG AACCCATTTG
    TACATCCATC ACGCCATTGA CTTGGCAACA GAAGAGGTGT CGCAGATGCA GCTATGCTCC
    CAGGCTGCAG AGGAGCTCAT TACTAGGATA TGTGACGCAG CCACAATTCA CTGTCTTTTG
    GAGCAAGAAC TGGCCCATGC TGTGAATGCC TGCTCCCATG CCCTGAATAA AGCCAACCCA
    AGGTGCCCGG AGAGTCTTAC AAGAGACACT GCCACTGAAA TAGCCATCAA TGTGAAGGCG
    CTGTATAATG AAACAGAATC TTTGCTAGTT GGCAGGGTTC CTTTGCAGCT GGAATCGCCA
    CATGAAGAGC GAGTATCCAA TGCCTTACAC TCTGTGGAGG TGGAATTACA GAAACTGACA
    GAGATTCCTT GGCTTTATTA TATCTTACAC CCAAATGAGG ATGAGGAACC TCCTATGGAT
    TGCACCAAAA GGAACAACAG AAGCACCGTA TTTCGAATAG TGCCAAAGTT TAAAAAGGAA
    AAGGTTCAGA AGCAGAAGAC AAGTTCACAG CCTGTTCAGA AATGGGGCAC AGAGGAAGTT
    GCTGCTTGGC TGGATCTGCT CAATTTGGGA GAGTACAAAG ATATCTTCAT CCGTCATGAC
    ATCAGAGGGG CTGAACTTTT GCATCTGGAA AGGCGAGATC TTAAGGATCT GGGGATACCG
    AAAGTGGGTC ATGTGAAGCG AATTCTCCAG GGAATTAAAG AGCTTGGAAG GAGCACTCCA
    CAGTCGGAGG TGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001191435.1
    CDS133..3387
    Translation

    Target ORF information:

    RefSeq Version NM_001204506.2
    Organism Homo sapiens(human)
    Definition Homo sapiens diacylglycerol kinase eta (DGKH), transcript variant 5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001204506.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGCTGTGTG CTGAGAACAG AAAGGAGATG GAGGATTGGA TCAGCTCACT GAAGTCTGTA 
    CAGACCAGAG AACCCTACGA GGTGGCCCAG TTTAATGTGG AACATTTCTC AGGGATGCAC
    AACTGGTACG CCTGCTCCCA CGCCCGACCC ACCTTCTGTA ACGTGTGCAG AGAGAGTCTT
    TCTGGAGTCA CCTCCCATGG CCTGTCCTGC GAAGTGTGTA AATTCAAGGC TCACAAAAGA
    TGTGCAGTGA GAGCAACAAA TAACTGTAAA TGGACTACCC TGGCCTCCAT CGGGAAGGAC
    ATTATAGAAG ATGAAGATGG CGTCGCGATG CCTCACCAGT GGCTTGAGGG CAACCTGCCT
    GTAAGTGCCA AGTGTGCTGT CTGCGACAAA ACATGTGGCA GTGTTCTCCG TCTACAGGAT
    TGGAAATGCC TTTGGTGTAA GACAATGGTA CACACTGCCT GCAAAGATTT ATACCATCCA
    ATATGTCCAC TTGGTCAATG TAAAGTATCT ATCATACCTC CAATTGCACT AAACAGCACC
    GATTCCGATG GTTTCTGTAG AGCAACATTT TCGTTCTGTG TTAGTCCTCT ATTGGTTTTT
    GTCAATTCTA AGAGTGGAGA TAATCAGGGA GTAAAGTTCC TCCGTCGCTT TAAACAGTTG
    CTAAATCCGG CTCAGGTGTT TGATTTAATG AATGGAGGTC CTCATTTAGG TTTAAGATTA
    TTTCAGAAGT TTGACAATTT CCGGATTCTT GTTTGTGGAG GCGATGGAAG TGTAGGTTGG
    GTTTTGTCAG AAATCGATAA GCTCAACTTG AATAAACAGT GTCAGCTGGG AGTGTTGCCT
    TTGGGTACAG GAAATGACCT TGCCCGAGTT CTTGGCTGGG GAGGTTCATA TGACGATGAC
    ACCCAACTTC CTCAGATCCT AGAGAAACTG GAACGAGCCA GTACCAAAAT GTTGGACAGG
    TGGAGTATAA TGACATATGA ACTCAAATTG CCACCAAAAG CTTCCCTACT TCCAGGACCT
    CCAGAAGCAT CTGAAGAATT TTATATGACG ATTTATGAAG ACTCAGTTGC AACGCATCTT
    ACAAAAATCC TCAATTCTGA TGAACATGCA GTGGTCATAT CTTCTGCCAA GACGCTATGT
    GAAACTGTAA AGGACTTCGT TGCCAAAGTA GAAAAGACGT ATGACAAAAC CTTGGAAAAT
    GCCGTTGTAG CTGATGCCGT GGCCAGTAAA TGTTCAGTCC TAAACGAGAA GCTCGAACAA
    CTGCTGCAGG CTTTGCACAC AGATTCCCAG GCTGCGCCTG TTCTCCCTGG CCTCAGCCCT
    CTCATTGTGG AAGAAGATGC TGTGGAATCG TCCAGTGAAG AGTCCCTGGG TGAAAGCAAA
    GAGCAGCTTG GGGATGACGT TACAAAACCT TCCTCCCAGA AAGCCGTCAA ACCAAGGGAA
    ATCATGTTGC GGGCAAATAG TTTAAAGAAA GCAGTGAGGC AAGTCATTGA GGAAGCCGGA
    AAAGTTATGG ATGACCCGAC AGTTCACCCC TGTGAACCAG CTAATCAGTC CTCTGATTAT
    GACAGCACAG AAACAGATGA ATCTAAGGAG GAAGCTAAAG ATGATGGTGC CAAAGAATCA
    ATAACTGTTA AAACTGCACC TCGGTCTCCA GATGCCCGGG CAAGTTATGG CCATTCCCAA
    ACTGATTCTG TCCCTGGTCC AGCTGTGGCA GCCAGCAAAG AAAACCTCCC TGTGCTCAAT
    ACCAGAATAA TCTGCCCAGG TTTAAGAGCA GGACTGGCTG CCTCAATTGC TGGGAGTTCG
    ATTATCAACA AAATGTTACT GGCAAACATT GATCCTTTTG GTGCCACGCC GTTTATTGAC
    CCGGATCTAG ATTCCGTAGA TGGATATTCA GAAAAATGTG TCATGAACAA TTACTTTGGG
    ATTGGATTAG ATGCAAAAAT TTCATTAGAA TTTAATAATA AAAGAGAGGA GCACCCTGAA
    AAATGCAGGA GCCGAACTAA AAACTTGATG TGGTATGGAG TCCTTGGAAC CCGGGAGTTA
    TTACAGAGAT CGTACAAGAA TTTAGAACAA AGGGTTCAAC TTGAGTGTGA TGGGCAGTAT
    ATTCCTCTTC CCAGCTTGCA AGGCATAGCC GTGTTGAACA TTCCCAGCTA TGCTGGAGGC
    ACTAACTTTT GGGGTGGAAC TAAAGAGGAT GATATATTTG CTGCACCATC CTTTGATGAC
    AAGATCCTGG AAGTTGTAGC AATATTTGAT AGCATGCAAA TGGCAGTTTC AAGGGTCATT
    AAACTGCAGC ATCATCGAAT AGCCCAGTGC CGTACAGTGA AAATCACTAT ATTTGGTGAC
    GAAGGAGTCC CAGTGCAAGT GGATGGTGAA GCGTGGGTTC AGCCTCCAGG GATTATCAAA
    ATTGTGCACA AAAACAGAGC ACAAATGCTA ACAAGGGACA GAGCCTTTGA GAGCACTCTG
    AAATCTTGGG AAGATAAGCA GAAGTGTGAT TCTGGTAAAC CAGTTCTCCG AACCCATTTG
    TACATCCATC ACGCCATTGA CTTGGCAACA GAAGAGGTGT CGCAGATGCA GCTATGCTCC
    CAGGCTGCAG AGGAGCTCAT TACTAGGATA TGTGACGCAG CCACAATTCA CTGTCTTTTG
    GAGCAAGAAC TGGCCCATGC TGTGAATGCC TGCTCCCATG CCCTGAATAA AGCCAACCCA
    AGGTGCCCGG AGAGTCTTAC AAGAGACACT GCCACTGAAA TAGCCATCAA TGTGAAGGCG
    CTGTATAATG AAACAGAATC TTTGCTAGTT GGCAGGGTTC CTTTGCAGCT GGAATCGCCA
    CATGAAGAGC GAGTATCCAA TGCCTTACAC TCTGTGGAGG TGGAATTACA GAAACTGACA
    GAGATTCCTT GGCTTTATTA TATCTTACAC CCAAATGAGG ATGAGGAACC TCCTATGGAT
    TGCACCAAAA GGAACAACAG AAGCACCGTA TTTCGAATAG TGCCAAAGTT TAAAAAGGAA
    AAGGTTCAGA AGCAGAAGAC AAGTTCACAG CCTGTTCAGA AATGGGGCAC AGAGGAAGTT
    GCTGCTTGGC TGGATCTGCT CAATTTGGGA GAGTACAAAG ATATCTTCAT CCGTCATGAC
    ATCAGAGGGG CTGAACTTTT GCATCTGGAA AGGCGAGATC TTAAGGATCT GGGGATACCG
    AAAGTGGGTC ATGTGAAGCG AATTCTCCAG GGAATTAAAG AGCTTGGAAG GAGCACTCCA
    CAGTCGGAGG TGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu28741
    Clone ID Related Accession (Same CDS sequence) NM_001204506.2 , NM_001204506.3
    Accession Version NM_001204506.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3255bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-28
    Organism Homo sapiens(human)
    Product diacylglycerol kinase eta isoform 4
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK302727.1, AB078967.1, BC043292.2, AL136527.9 and BM978856.1. On Jun 2, 2019 this sequence version replaced NM_001204506.2. Transcript Variant: This variant (5) differs in the 5' UTR, lacks a portion of the 5' coding region, and contains an alternate exon in the 3' coding region, which results in a frameshift, compared to variant 1. It initiates translation at a downstream in-frame start codon. The encoded isoform (4) has a shorter N-terminus and a distinct C-terminus, and is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK302727.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2148093 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGCTGTGTG CTGAGAACAG AAAGGAGATG GAGGATTGGA TCAGCTCACT GAAGTCTGTA 
    CAGACCAGAG AACCCTACGA GGTGGCCCAG TTTAATGTGG AACATTTCTC AGGGATGCAC
    AACTGGTACG CCTGCTCCCA CGCCCGACCC ACCTTCTGTA ACGTGTGCAG AGAGAGTCTT
    TCTGGAGTCA CCTCCCATGG CCTGTCCTGC GAAGTGTGTA AATTCAAGGC TCACAAAAGA
    TGTGCAGTGA GAGCAACAAA TAACTGTAAA TGGACTACCC TGGCCTCCAT CGGGAAGGAC
    ATTATAGAAG ATGAAGATGG CGTCGCGATG CCTCACCAGT GGCTTGAGGG CAACCTGCCT
    GTAAGTGCCA AGTGTGCTGT CTGCGACAAA ACATGTGGCA GTGTTCTCCG TCTACAGGAT
    TGGAAATGCC TTTGGTGTAA GACAATGGTA CACACTGCCT GCAAAGATTT ATACCATCCA
    ATATGTCCAC TTGGTCAATG TAAAGTATCT ATCATACCTC CAATTGCACT AAACAGCACC
    GATTCCGATG GTTTCTGTAG AGCAACATTT TCGTTCTGTG TTAGTCCTCT ATTGGTTTTT
    GTCAATTCTA AGAGTGGAGA TAATCAGGGA GTAAAGTTCC TCCGTCGCTT TAAACAGTTG
    CTAAATCCGG CTCAGGTGTT TGATTTAATG AATGGAGGTC CTCATTTAGG TTTAAGATTA
    TTTCAGAAGT TTGACAATTT CCGGATTCTT GTTTGTGGAG GCGATGGAAG TGTAGGTTGG
    GTTTTGTCAG AAATCGATAA GCTCAACTTG AATAAACAGT GTCAGCTGGG AGTGTTGCCT
    TTGGGTACAG GAAATGACCT TGCCCGAGTT CTTGGCTGGG GAGGTTCATA TGACGATGAC
    ACCCAACTTC CTCAGATCCT AGAGAAACTG GAACGAGCCA GTACCAAAAT GTTGGACAGG
    TGGAGTATAA TGACATATGA ACTCAAATTG CCACCAAAAG CTTCCCTACT TCCAGGACCT
    CCAGAAGCAT CTGAAGAATT TTATATGACG ATTTATGAAG ACTCAGTTGC AACGCATCTT
    ACAAAAATCC TCAATTCTGA TGAACATGCA GTGGTCATAT CTTCTGCCAA GACGCTATGT
    GAAACTGTAA AGGACTTCGT TGCCAAAGTA GAAAAGACGT ATGACAAAAC CTTGGAAAAT
    GCCGTTGTAG CTGATGCCGT GGCCAGTAAA TGTTCAGTCC TAAACGAGAA GCTCGAACAA
    CTGCTGCAGG CTTTGCACAC AGATTCCCAG GCTGCGCCTG TTCTCCCTGG CCTCAGCCCT
    CTCATTGTGG AAGAAGATGC TGTGGAATCG TCCAGTGAAG AGTCCCTGGG TGAAAGCAAA
    GAGCAGCTTG GGGATGACGT TACAAAACCT TCCTCCCAGA AAGCCGTCAA ACCAAGGGAA
    ATCATGTTGC GGGCAAATAG TTTAAAGAAA GCAGTGAGGC AAGTCATTGA GGAAGCCGGA
    AAAGTTATGG ATGACCCGAC AGTTCACCCC TGTGAACCAG CTAATCAGTC CTCTGATTAT
    GACAGCACAG AAACAGATGA ATCTAAGGAG GAAGCTAAAG ATGATGGTGC CAAAGAATCA
    ATAACTGTTA AAACTGCACC TCGGTCTCCA GATGCCCGGG CAAGTTATGG CCATTCCCAA
    ACTGATTCTG TCCCTGGTCC AGCTGTGGCA GCCAGCAAAG AAAACCTCCC TGTGCTCAAT
    ACCAGAATAA TCTGCCCAGG TTTAAGAGCA GGACTGGCTG CCTCAATTGC TGGGAGTTCG
    ATTATCAACA AAATGTTACT GGCAAACATT GATCCTTTTG GTGCCACGCC GTTTATTGAC
    CCGGATCTAG ATTCCGTAGA TGGATATTCA GAAAAATGTG TCATGAACAA TTACTTTGGG
    ATTGGATTAG ATGCAAAAAT TTCATTAGAA TTTAATAATA AAAGAGAGGA GCACCCTGAA
    AAATGCAGGA GCCGAACTAA AAACTTGATG TGGTATGGAG TCCTTGGAAC CCGGGAGTTA
    TTACAGAGAT CGTACAAGAA TTTAGAACAA AGGGTTCAAC TTGAGTGTGA TGGGCAGTAT
    ATTCCTCTTC CCAGCTTGCA AGGCATAGCC GTGTTGAACA TTCCCAGCTA TGCTGGAGGC
    ACTAACTTTT GGGGTGGAAC TAAAGAGGAT GATATATTTG CTGCACCATC CTTTGATGAC
    AAGATCCTGG AAGTTGTAGC AATATTTGAT AGCATGCAAA TGGCAGTTTC AAGGGTCATT
    AAACTGCAGC ATCATCGAAT AGCCCAGTGC CGTACAGTGA AAATCACTAT ATTTGGTGAC
    GAAGGAGTCC CAGTGCAAGT GGATGGTGAA GCGTGGGTTC AGCCTCCAGG GATTATCAAA
    ATTGTGCACA AAAACAGAGC ACAAATGCTA ACAAGGGACA GAGCCTTTGA GAGCACTCTG
    AAATCTTGGG AAGATAAGCA GAAGTGTGAT TCTGGTAAAC CAGTTCTCCG AACCCATTTG
    TACATCCATC ACGCCATTGA CTTGGCAACA GAAGAGGTGT CGCAGATGCA GCTATGCTCC
    CAGGCTGCAG AGGAGCTCAT TACTAGGATA TGTGACGCAG CCACAATTCA CTGTCTTTTG
    GAGCAAGAAC TGGCCCATGC TGTGAATGCC TGCTCCCATG CCCTGAATAA AGCCAACCCA
    AGGTGCCCGG AGAGTCTTAC AAGAGACACT GCCACTGAAA TAGCCATCAA TGTGAAGGCG
    CTGTATAATG AAACAGAATC TTTGCTAGTT GGCAGGGTTC CTTTGCAGCT GGAATCGCCA
    CATGAAGAGC GAGTATCCAA TGCCTTACAC TCTGTGGAGG TGGAATTACA GAAACTGACA
    GAGATTCCTT GGCTTTATTA TATCTTACAC CCAAATGAGG ATGAGGAACC TCCTATGGAT
    TGCACCAAAA GGAACAACAG AAGCACCGTA TTTCGAATAG TGCCAAAGTT TAAAAAGGAA
    AAGGTTCAGA AGCAGAAGAC AAGTTCACAG CCTGTTCAGA AATGGGGCAC AGAGGAAGTT
    GCTGCTTGGC TGGATCTGCT CAATTTGGGA GAGTACAAAG ATATCTTCAT CCGTCATGAC
    ATCAGAGGGG CTGAACTTTT GCATCTGGAA AGGCGAGATC TTAAGGATCT GGGGATACCG
    AAAGTGGGTC ATGTGAAGCG AATTCTCCAG GGAATTAAAG AGCTTGGAAG GAGCACTCCA
    CAGTCGGAGG TGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001191435.1
    CDS117..3371
    Translation

    Target ORF information:

    RefSeq Version NM_001204506.3
    Organism Homo sapiens(human)
    Definition Homo sapiens diacylglycerol kinase eta (DGKH), transcript variant 5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001204506.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGCTGTGTG CTGAGAACAG AAAGGAGATG GAGGATTGGA TCAGCTCACT GAAGTCTGTA 
    CAGACCAGAG AACCCTACGA GGTGGCCCAG TTTAATGTGG AACATTTCTC AGGGATGCAC
    AACTGGTACG CCTGCTCCCA CGCCCGACCC ACCTTCTGTA ACGTGTGCAG AGAGAGTCTT
    TCTGGAGTCA CCTCCCATGG CCTGTCCTGC GAAGTGTGTA AATTCAAGGC TCACAAAAGA
    TGTGCAGTGA GAGCAACAAA TAACTGTAAA TGGACTACCC TGGCCTCCAT CGGGAAGGAC
    ATTATAGAAG ATGAAGATGG CGTCGCGATG CCTCACCAGT GGCTTGAGGG CAACCTGCCT
    GTAAGTGCCA AGTGTGCTGT CTGCGACAAA ACATGTGGCA GTGTTCTCCG TCTACAGGAT
    TGGAAATGCC TTTGGTGTAA GACAATGGTA CACACTGCCT GCAAAGATTT ATACCATCCA
    ATATGTCCAC TTGGTCAATG TAAAGTATCT ATCATACCTC CAATTGCACT AAACAGCACC
    GATTCCGATG GTTTCTGTAG AGCAACATTT TCGTTCTGTG TTAGTCCTCT ATTGGTTTTT
    GTCAATTCTA AGAGTGGAGA TAATCAGGGA GTAAAGTTCC TCCGTCGCTT TAAACAGTTG
    CTAAATCCGG CTCAGGTGTT TGATTTAATG AATGGAGGTC CTCATTTAGG TTTAAGATTA
    TTTCAGAAGT TTGACAATTT CCGGATTCTT GTTTGTGGAG GCGATGGAAG TGTAGGTTGG
    GTTTTGTCAG AAATCGATAA GCTCAACTTG AATAAACAGT GTCAGCTGGG AGTGTTGCCT
    TTGGGTACAG GAAATGACCT TGCCCGAGTT CTTGGCTGGG GAGGTTCATA TGACGATGAC
    ACCCAACTTC CTCAGATCCT AGAGAAACTG GAACGAGCCA GTACCAAAAT GTTGGACAGG
    TGGAGTATAA TGACATATGA ACTCAAATTG CCACCAAAAG CTTCCCTACT TCCAGGACCT
    CCAGAAGCAT CTGAAGAATT TTATATGACG ATTTATGAAG ACTCAGTTGC AACGCATCTT
    ACAAAAATCC TCAATTCTGA TGAACATGCA GTGGTCATAT CTTCTGCCAA GACGCTATGT
    GAAACTGTAA AGGACTTCGT TGCCAAAGTA GAAAAGACGT ATGACAAAAC CTTGGAAAAT
    GCCGTTGTAG CTGATGCCGT GGCCAGTAAA TGTTCAGTCC TAAACGAGAA GCTCGAACAA
    CTGCTGCAGG CTTTGCACAC AGATTCCCAG GCTGCGCCTG TTCTCCCTGG CCTCAGCCCT
    CTCATTGTGG AAGAAGATGC TGTGGAATCG TCCAGTGAAG AGTCCCTGGG TGAAAGCAAA
    GAGCAGCTTG GGGATGACGT TACAAAACCT TCCTCCCAGA AAGCCGTCAA ACCAAGGGAA
    ATCATGTTGC GGGCAAATAG TTTAAAGAAA GCAGTGAGGC AAGTCATTGA GGAAGCCGGA
    AAAGTTATGG ATGACCCGAC AGTTCACCCC TGTGAACCAG CTAATCAGTC CTCTGATTAT
    GACAGCACAG AAACAGATGA ATCTAAGGAG GAAGCTAAAG ATGATGGTGC CAAAGAATCA
    ATAACTGTTA AAACTGCACC TCGGTCTCCA GATGCCCGGG CAAGTTATGG CCATTCCCAA
    ACTGATTCTG TCCCTGGTCC AGCTGTGGCA GCCAGCAAAG AAAACCTCCC TGTGCTCAAT
    ACCAGAATAA TCTGCCCAGG TTTAAGAGCA GGACTGGCTG CCTCAATTGC TGGGAGTTCG
    ATTATCAACA AAATGTTACT GGCAAACATT GATCCTTTTG GTGCCACGCC GTTTATTGAC
    CCGGATCTAG ATTCCGTAGA TGGATATTCA GAAAAATGTG TCATGAACAA TTACTTTGGG
    ATTGGATTAG ATGCAAAAAT TTCATTAGAA TTTAATAATA AAAGAGAGGA GCACCCTGAA
    AAATGCAGGA GCCGAACTAA AAACTTGATG TGGTATGGAG TCCTTGGAAC CCGGGAGTTA
    TTACAGAGAT CGTACAAGAA TTTAGAACAA AGGGTTCAAC TTGAGTGTGA TGGGCAGTAT
    ATTCCTCTTC CCAGCTTGCA AGGCATAGCC GTGTTGAACA TTCCCAGCTA TGCTGGAGGC
    ACTAACTTTT GGGGTGGAAC TAAAGAGGAT GATATATTTG CTGCACCATC CTTTGATGAC
    AAGATCCTGG AAGTTGTAGC AATATTTGAT AGCATGCAAA TGGCAGTTTC AAGGGTCATT
    AAACTGCAGC ATCATCGAAT AGCCCAGTGC CGTACAGTGA AAATCACTAT ATTTGGTGAC
    GAAGGAGTCC CAGTGCAAGT GGATGGTGAA GCGTGGGTTC AGCCTCCAGG GATTATCAAA
    ATTGTGCACA AAAACAGAGC ACAAATGCTA ACAAGGGACA GAGCCTTTGA GAGCACTCTG
    AAATCTTGGG AAGATAAGCA GAAGTGTGAT TCTGGTAAAC CAGTTCTCCG AACCCATTTG
    TACATCCATC ACGCCATTGA CTTGGCAACA GAAGAGGTGT CGCAGATGCA GCTATGCTCC
    CAGGCTGCAG AGGAGCTCAT TACTAGGATA TGTGACGCAG CCACAATTCA CTGTCTTTTG
    GAGCAAGAAC TGGCCCATGC TGTGAATGCC TGCTCCCATG CCCTGAATAA AGCCAACCCA
    AGGTGCCCGG AGAGTCTTAC AAGAGACACT GCCACTGAAA TAGCCATCAA TGTGAAGGCG
    CTGTATAATG AAACAGAATC TTTGCTAGTT GGCAGGGTTC CTTTGCAGCT GGAATCGCCA
    CATGAAGAGC GAGTATCCAA TGCCTTACAC TCTGTGGAGG TGGAATTACA GAAACTGACA
    GAGATTCCTT GGCTTTATTA TATCTTACAC CCAAATGAGG ATGAGGAACC TCCTATGGAT
    TGCACCAAAA GGAACAACAG AAGCACCGTA TTTCGAATAG TGCCAAAGTT TAAAAAGGAA
    AAGGTTCAGA AGCAGAAGAC AAGTTCACAG CCTGTTCAGA AATGGGGCAC AGAGGAAGTT
    GCTGCTTGGC TGGATCTGCT CAATTTGGGA GAGTACAAAG ATATCTTCAT CCGTCATGAC
    ATCAGAGGGG CTGAACTTTT GCATCTGGAA AGGCGAGATC TTAAGGATCT GGGGATACCG
    AAAGTGGGTC ATGTGAAGCG AATTCTCCAG GGAATTAAAG AGCTTGGAAG GAGCACTCCA
    CAGTCGGAGG TGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu28845
    Clone ID Related Accession (Same CDS sequence) NM_178009.4 , NM_178009.5
    Accession Version NM_178009.4 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3663bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-01-26
    Organism Homo sapiens(human)
    Product diacylglycerol kinase eta isoform 2
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DR002010.1, AB078968.1, AK302727.1, BC043292.2, AL136527.9 and BM978856.1. This sequence is a reference standard in the RefSeqGene project. On Jul 2, 2014 this sequence version replaced NM_178009.3. Transcript Variant: This variant (2) contains an alternate exon in the 3' coding region, which results in a frameshift, compared to variant 1. The encoded isoform (2) has a longer and distinct C-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB078968.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2142348 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCAGGGG CCGGAGGCCA GCACCACCCT CCGGGCGCCG CTGGAGGAGC GGCCGCCGGA 
    GCCGGCGCCG CGGTCACCTC CGCCGCTGCC TCGGCGGGGC CGGGAGAGGA TTCGTCTGAC
    AGCGAAGCGG AGCAAGAGGG ACCCCAGAAA CTGATCCGCA AAGTGTCTAC CTCGGGGCAG
    ATCCGGACCA AGACCAGTAT TAAAGAGGGA CAGCTATTGA AGCAAACCAG TTCTTTCCAA
    AGGTGGAAAA AGCGATACTT CAAACTTCGA GGCCGCACCC TTTACTATGC AAAGGACTCA
    AAGTCTCTGA TATTTGATGA AGTTGACCTC TCAGATGCTA GTGTAGCTGA AGCAAGCACG
    AAAAATGCTA ACAACAGCTT CACGATCATC ACTCCATTCA GAAGGCTAAT GCTGTGTGCT
    GAGAACAGAA AGGAGATGGA GGATTGGATC AGCTCACTGA AGTCTGTACA GACCAGAGAA
    CCCTACGAGG TGGCCCAGTT TAATGTGGAA CATTTCTCAG GGATGCACAA CTGGTACGCC
    TGCTCCCACG CCCGACCCAC CTTCTGTAAC GTGTGCAGAG AGAGTCTTTC TGGAGTCACC
    TCCCATGGCC TGTCCTGCGA AGTGTGTAAA TTCAAGGCTC ACAAAAGATG TGCAGTGAGA
    GCAACAAATA ACTGTAAATG GACTACCCTG GCCTCCATCG GGAAGGACAT TATAGAAGAT
    GAAGATGGCG TCGCGATGCC TCACCAGTGG CTTGAGGGCA ACCTGCCTGT AAGTGCCAAG
    TGTGCTGTCT GCGACAAAAC ATGTGGCAGT GTTCTCCGTC TACAGGATTG GAAATGCCTT
    TGGTGTAAGA CAATGGTACA CACTGCCTGC AAAGATTTAT ACCATCCAAT ATGTCCACTT
    GGTCAATGTA AAGTATCTAT CATACCTCCA ATTGCACTAA ACAGCACCGA TTCCGATGGT
    TTCTGTAGAG CAACATTTTC GTTCTGTGTT AGTCCTCTAT TGGTTTTTGT CAATTCTAAG
    AGTGGAGATA ATCAGGGAGT AAAGTTCCTC CGTCGCTTTA AACAGTTGCT AAATCCGGCT
    CAGGTGTTTG ATTTAATGAA TGGAGGTCCT CATTTAGGTT TAAGATTATT TCAGAAGTTT
    GACAATTTCC GGATTCTTGT TTGTGGAGGC GATGGAAGTG TAGGTTGGGT TTTGTCAGAA
    ATCGATAAGC TCAACTTGAA TAAACAGTGT CAGCTGGGAG TGTTGCCTTT GGGTACAGGA
    AATGACCTTG CCCGAGTTCT TGGCTGGGGA GGTTCATATG ACGATGACAC CCAACTTCCT
    CAGATCCTAG AGAAACTGGA ACGAGCCAGT ACCAAAATGT TGGACAGGTG GAGTATAATG
    ACATATGAAC TCAAATTGCC ACCAAAAGCT TCCCTACTTC CAGGACCTCC AGAAGCATCT
    GAAGAATTTT ATATGACGAT TTATGAAGAC TCAGTTGCAA CGCATCTTAC AAAAATCCTC
    AATTCTGATG AACATGCAGT GGTCATATCT TCTGCCAAGA CGCTATGTGA AACTGTAAAG
    GACTTCGTTG CCAAAGTAGA AAAGACGTAT GACAAAACCT TGGAAAATGC CGTTGTAGCT
    GATGCCGTGG CCAGTAAATG TTCAGTCCTA AACGAGAAGC TCGAACAACT GCTGCAGGCT
    TTGCACACAG ATTCCCAGGC TGCGCCTGTT CTCCCTGGCC TCAGCCCTCT CATTGTGGAA
    GAAGATGCTG TGGAATCGTC CAGTGAAGAG TCCCTGGGTG AAAGCAAAGA GCAGCTTGGG
    GATGACGTTA CAAAACCTTC CTCCCAGAAA GCCGTCAAAC CAAGGGAAAT CATGTTGCGG
    GCAAATAGTT TAAAGAAAGC AGTGAGGCAA GTCATTGAGG AAGCCGGAAA AGTTATGGAT
    GACCCGACAG TTCACCCCTG TGAACCAGCT AATCAGTCCT CTGATTATGA CAGCACAGAA
    ACAGATGAAT CTAAGGAGGA AGCTAAAGAT GATGGTGCCA AAGAATCAAT AACTGTTAAA
    ACTGCACCTC GGTCTCCAGA TGCCCGGGCA AGTTATGGCC ATTCCCAAAC TGATTCTGTC
    CCTGGTCCAG CTGTGGCAGC CAGCAAAGAA AACCTCCCTG TGCTCAATAC CAGAATAATC
    TGCCCAGGTT TAAGAGCAGG ACTGGCTGCC TCAATTGCTG GGAGTTCGAT TATCAACAAA
    ATGTTACTGG CAAACATTGA TCCTTTTGGT GCCACGCCGT TTATTGACCC GGATCTAGAT
    TCCGTAGATG GATATTCAGA AAAATGTGTC ATGAACAATT ACTTTGGGAT TGGATTAGAT
    GCAAAAATTT CATTAGAATT TAATAATAAA AGAGAGGAGC ACCCTGAAAA ATGCAGGAGC
    CGAACTAAAA ACTTGATGTG GTATGGAGTC CTTGGAACCC GGGAGTTATT ACAGAGATCG
    TACAAGAATT TAGAACAAAG GGTTCAACTT GAGTGTGATG GGCAGTATAT TCCTCTTCCC
    AGCTTGCAAG GCATAGCCGT GTTGAACATT CCCAGCTATG CTGGAGGCAC TAACTTTTGG
    GGTGGAACTA AAGAGGATGA TATATTTGCT GCACCATCCT TTGATGACAA GATCCTGGAA
    GTTGTAGCAA TATTTGATAG CATGCAAATG GCAGTTTCAA GGGTCATTAA ACTGCAGCAT
    CATCGAATAG CCCAGTGCCG TACAGTGAAA ATCACTATAT TTGGTGACGA AGGAGTCCCA
    GTGCAAGTGG ATGGTGAAGC GTGGGTTCAG CCTCCAGGGA TTATCAAAAT TGTGCACAAA
    AACAGAGCAC AAATGCTAAC AAGGGACAGA GCCTTTGAGA GCACTCTGAA ATCTTGGGAA
    GATAAGCAGA AGTGTGATTC TGGTAAACCA GTTCTCCGAA CCCATTTGTA CATCCATCAC
    GCCATTGACT TGGCAACAGA AGAGGTGTCG CAGATGCAGC TATGCTCCCA GGCTGCAGAG
    GAGCTCATTA CTAGGATATG TGACGCAGCC ACAATTCACT GTCTTTTGGA GCAAGAACTG
    GCCCATGCTG TGAATGCCTG CTCCCATGCC CTGAATAAAG CCAACCCAAG GTGCCCGGAG
    AGTCTTACAA GAGACACTGC CACTGAAATA GCCATCAATG TGAAGGCGCT GTATAATGAA
    ACAGAATCTT TGCTAGTTGG CAGGGTTCCT TTGCAGCTGG AATCGCCACA TGAAGAGCGA
    GTATCCAATG CCTTACACTC TGTGGAGGTG GAATTACAGA AACTGACAGA GATTCCTTGG
    CTTTATTATA TCTTACACCC AAATGAGGAT GAGGAACCTC CTATGGATTG CACCAAAAGG
    AACAACAGAA GCACCGTATT TCGAATAGTG CCAAAGTTTA AAAAGGAAAA GGTTCAGAAG
    CAGAAGACAA GTTCACAGCC TGTTCAGAAA TGGGGCACAG AGGAAGTTGC TGCTTGGCTG
    GATCTGCTCA ATTTGGGAGA GTACAAAGAT ATCTTCATCC GTCATGACAT CAGAGGGGCT
    GAACTTTTGC ATCTGGAAAG GCGAGATCTT AAGGATCTGG GGATACCGAA AGTGGGTCAT
    GTGAAGCGAA TTCTCCAGGG AATTAAAGAG CTTGGAAGGA GCACTCCACA GTCGGAGGTG
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_821077.1
    CDS67..3729
    Translation

    Target ORF information:

    RefSeq Version NM_178009.4
    Organism Homo sapiens(human)
    Definition Homo sapiens diacylglycerol kinase eta (DGKH), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_178009.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCAGGGG CCGGAGGCCA GCACCACCCT CCGGGCGCCG CTGGAGGAGC GGCCGCCGGA 
    GCCGGCGCCG CGGTCACCTC CGCCGCTGCC TCGGCGGGGC CGGGAGAGGA TTCGTCTGAC
    AGCGAAGCGG AGCAAGAGGG ACCCCAGAAA CTGATCCGCA AAGTGTCTAC CTCGGGGCAG
    ATCCGGACCA AGACCAGTAT TAAAGAGGGA CAGCTATTGA AGCAAACCAG TTCTTTCCAA
    AGGTGGAAAA AGCGATACTT CAAACTTCGA GGCCGCACCC TTTACTATGC AAAGGACTCA
    AAGTCTCTGA TATTTGATGA AGTTGACCTC TCAGATGCTA GTGTAGCTGA AGCAAGCACG
    AAAAATGCTA ACAACAGCTT CACGATCATC ACTCCATTCA GAAGGCTAAT GCTGTGTGCT
    GAGAACAGAA AGGAGATGGA GGATTGGATC AGCTCACTGA AGTCTGTACA GACCAGAGAA
    CCCTACGAGG TGGCCCAGTT TAATGTGGAA CATTTCTCAG GGATGCACAA CTGGTACGCC
    TGCTCCCACG CCCGACCCAC CTTCTGTAAC GTGTGCAGAG AGAGTCTTTC TGGAGTCACC
    TCCCATGGCC TGTCCTGCGA AGTGTGTAAA TTCAAGGCTC ACAAAAGATG TGCAGTGAGA
    GCAACAAATA ACTGTAAATG GACTACCCTG GCCTCCATCG GGAAGGACAT TATAGAAGAT
    GAAGATGGCG TCGCGATGCC TCACCAGTGG CTTGAGGGCA ACCTGCCTGT AAGTGCCAAG
    TGTGCTGTCT GCGACAAAAC ATGTGGCAGT GTTCTCCGTC TACAGGATTG GAAATGCCTT
    TGGTGTAAGA CAATGGTACA CACTGCCTGC AAAGATTTAT ACCATCCAAT ATGTCCACTT
    GGTCAATGTA AAGTATCTAT CATACCTCCA ATTGCACTAA ACAGCACCGA TTCCGATGGT
    TTCTGTAGAG CAACATTTTC GTTCTGTGTT AGTCCTCTAT TGGTTTTTGT CAATTCTAAG
    AGTGGAGATA ATCAGGGAGT AAAGTTCCTC CGTCGCTTTA AACAGTTGCT AAATCCGGCT
    CAGGTGTTTG ATTTAATGAA TGGAGGTCCT CATTTAGGTT TAAGATTATT TCAGAAGTTT
    GACAATTTCC GGATTCTTGT TTGTGGAGGC GATGGAAGTG TAGGTTGGGT TTTGTCAGAA
    ATCGATAAGC TCAACTTGAA TAAACAGTGT CAGCTGGGAG TGTTGCCTTT GGGTACAGGA
    AATGACCTTG CCCGAGTTCT TGGCTGGGGA GGTTCATATG ACGATGACAC CCAACTTCCT
    CAGATCCTAG AGAAACTGGA ACGAGCCAGT ACCAAAATGT TGGACAGGTG GAGTATAATG
    ACATATGAAC TCAAATTGCC ACCAAAAGCT TCCCTACTTC CAGGACCTCC AGAAGCATCT
    GAAGAATTTT ATATGACGAT TTATGAAGAC TCAGTTGCAA CGCATCTTAC AAAAATCCTC
    AATTCTGATG AACATGCAGT GGTCATATCT TCTGCCAAGA CGCTATGTGA AACTGTAAAG
    GACTTCGTTG CCAAAGTAGA AAAGACGTAT GACAAAACCT TGGAAAATGC CGTTGTAGCT
    GATGCCGTGG CCAGTAAATG TTCAGTCCTA AACGAGAAGC TCGAACAACT GCTGCAGGCT
    TTGCACACAG ATTCCCAGGC TGCGCCTGTT CTCCCTGGCC TCAGCCCTCT CATTGTGGAA
    GAAGATGCTG TGGAATCGTC CAGTGAAGAG TCCCTGGGTG AAAGCAAAGA GCAGCTTGGG
    GATGACGTTA CAAAACCTTC CTCCCAGAAA GCCGTCAAAC CAAGGGAAAT CATGTTGCGG
    GCAAATAGTT TAAAGAAAGC AGTGAGGCAA GTCATTGAGG AAGCCGGAAA AGTTATGGAT
    GACCCGACAG TTCACCCCTG TGAACCAGCT AATCAGTCCT CTGATTATGA CAGCACAGAA
    ACAGATGAAT CTAAGGAGGA AGCTAAAGAT GATGGTGCCA AAGAATCAAT AACTGTTAAA
    ACTGCACCTC GGTCTCCAGA TGCCCGGGCA AGTTATGGCC ATTCCCAAAC TGATTCTGTC
    CCTGGTCCAG CTGTGGCAGC CAGCAAAGAA AACCTCCCTG TGCTCAATAC CAGAATAATC
    TGCCCAGGTT TAAGAGCAGG ACTGGCTGCC TCAATTGCTG GGAGTTCGAT TATCAACAAA
    ATGTTACTGG CAAACATTGA TCCTTTTGGT GCCACGCCGT TTATTGACCC GGATCTAGAT
    TCCGTAGATG GATATTCAGA AAAATGTGTC ATGAACAATT ACTTTGGGAT TGGATTAGAT
    GCAAAAATTT CATTAGAATT TAATAATAAA AGAGAGGAGC ACCCTGAAAA ATGCAGGAGC
    CGAACTAAAA ACTTGATGTG GTATGGAGTC CTTGGAACCC GGGAGTTATT ACAGAGATCG
    TACAAGAATT TAGAACAAAG GGTTCAACTT GAGTGTGATG GGCAGTATAT TCCTCTTCCC
    AGCTTGCAAG GCATAGCCGT GTTGAACATT CCCAGCTATG CTGGAGGCAC TAACTTTTGG
    GGTGGAACTA AAGAGGATGA TATATTTGCT GCACCATCCT TTGATGACAA GATCCTGGAA
    GTTGTAGCAA TATTTGATAG CATGCAAATG GCAGTTTCAA GGGTCATTAA ACTGCAGCAT
    CATCGAATAG CCCAGTGCCG TACAGTGAAA ATCACTATAT TTGGTGACGA AGGAGTCCCA
    GTGCAAGTGG ATGGTGAAGC GTGGGTTCAG CCTCCAGGGA TTATCAAAAT TGTGCACAAA
    AACAGAGCAC AAATGCTAAC AAGGGACAGA GCCTTTGAGA GCACTCTGAA ATCTTGGGAA
    GATAAGCAGA AGTGTGATTC TGGTAAACCA GTTCTCCGAA CCCATTTGTA CATCCATCAC
    GCCATTGACT TGGCAACAGA AGAGGTGTCG CAGATGCAGC TATGCTCCCA GGCTGCAGAG
    GAGCTCATTA CTAGGATATG TGACGCAGCC ACAATTCACT GTCTTTTGGA GCAAGAACTG
    GCCCATGCTG TGAATGCCTG CTCCCATGCC CTGAATAAAG CCAACCCAAG GTGCCCGGAG
    AGTCTTACAA GAGACACTGC CACTGAAATA GCCATCAATG TGAAGGCGCT GTATAATGAA
    ACAGAATCTT TGCTAGTTGG CAGGGTTCCT TTGCAGCTGG AATCGCCACA TGAAGAGCGA
    GTATCCAATG CCTTACACTC TGTGGAGGTG GAATTACAGA AACTGACAGA GATTCCTTGG
    CTTTATTATA TCTTACACCC AAATGAGGAT GAGGAACCTC CTATGGATTG CACCAAAAGG
    AACAACAGAA GCACCGTATT TCGAATAGTG CCAAAGTTTA AAAAGGAAAA GGTTCAGAAG
    CAGAAGACAA GTTCACAGCC TGTTCAGAAA TGGGGCACAG AGGAAGTTGC TGCTTGGCTG
    GATCTGCTCA ATTTGGGAGA GTACAAAGAT ATCTTCATCC GTCATGACAT CAGAGGGGCT
    GAACTTTTGC ATCTGGAAAG GCGAGATCTT AAGGATCTGG GGATACCGAA AGTGGGTCAT
    GTGAAGCGAA TTCTCCAGGG AATTAAAGAG CTTGGAAGGA GCACTCCACA GTCGGAGGTG
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu28845
    Clone ID Related Accession (Same CDS sequence) NM_178009.4 , NM_178009.5
    Accession Version NM_178009.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3663bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-28
    Organism Homo sapiens(human)
    Product diacylglycerol kinase eta isoform 2
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL157932.21, DR002010.1, AB078968.1, AK302727.1, BC043292.2, AL136527.9 and BM978856.1. This sequence is a reference standard in the RefSeqGene project. On Jun 2, 2019 this sequence version replaced NM_178009.4. Transcript Variant: This variant (2) contains an alternate exon in the 3' coding region, which results in a frameshift, compared to variant 1. The encoded isoform (2) has a longer and distinct C-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB078968.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2142348 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCAGGGG CCGGAGGCCA GCACCACCCT CCGGGCGCCG CTGGAGGAGC GGCCGCCGGA 
    GCCGGCGCCG CGGTCACCTC CGCCGCTGCC TCGGCGGGGC CGGGAGAGGA TTCGTCTGAC
    AGCGAAGCGG AGCAAGAGGG ACCCCAGAAA CTGATCCGCA AAGTGTCTAC CTCGGGGCAG
    ATCCGGACCA AGACCAGTAT TAAAGAGGGA CAGCTATTGA AGCAAACCAG TTCTTTCCAA
    AGGTGGAAAA AGCGATACTT CAAACTTCGA GGCCGCACCC TTTACTATGC AAAGGACTCA
    AAGTCTCTGA TATTTGATGA AGTTGACCTC TCAGATGCTA GTGTAGCTGA AGCAAGCACG
    AAAAATGCTA ACAACAGCTT CACGATCATC ACTCCATTCA GAAGGCTAAT GCTGTGTGCT
    GAGAACAGAA AGGAGATGGA GGATTGGATC AGCTCACTGA AGTCTGTACA GACCAGAGAA
    CCCTACGAGG TGGCCCAGTT TAATGTGGAA CATTTCTCAG GGATGCACAA CTGGTACGCC
    TGCTCCCACG CCCGACCCAC CTTCTGTAAC GTGTGCAGAG AGAGTCTTTC TGGAGTCACC
    TCCCATGGCC TGTCCTGCGA AGTGTGTAAA TTCAAGGCTC ACAAAAGATG TGCAGTGAGA
    GCAACAAATA ACTGTAAATG GACTACCCTG GCCTCCATCG GGAAGGACAT TATAGAAGAT
    GAAGATGGCG TCGCGATGCC TCACCAGTGG CTTGAGGGCA ACCTGCCTGT AAGTGCCAAG
    TGTGCTGTCT GCGACAAAAC ATGTGGCAGT GTTCTCCGTC TACAGGATTG GAAATGCCTT
    TGGTGTAAGA CAATGGTACA CACTGCCTGC AAAGATTTAT ACCATCCAAT ATGTCCACTT
    GGTCAATGTA AAGTATCTAT CATACCTCCA ATTGCACTAA ACAGCACCGA TTCCGATGGT
    TTCTGTAGAG CAACATTTTC GTTCTGTGTT AGTCCTCTAT TGGTTTTTGT CAATTCTAAG
    AGTGGAGATA ATCAGGGAGT AAAGTTCCTC CGTCGCTTTA AACAGTTGCT AAATCCGGCT
    CAGGTGTTTG ATTTAATGAA TGGAGGTCCT CATTTAGGTT TAAGATTATT TCAGAAGTTT
    GACAATTTCC GGATTCTTGT TTGTGGAGGC GATGGAAGTG TAGGTTGGGT TTTGTCAGAA
    ATCGATAAGC TCAACTTGAA TAAACAGTGT CAGCTGGGAG TGTTGCCTTT GGGTACAGGA
    AATGACCTTG CCCGAGTTCT TGGCTGGGGA GGTTCATATG ACGATGACAC CCAACTTCCT
    CAGATCCTAG AGAAACTGGA ACGAGCCAGT ACCAAAATGT TGGACAGGTG GAGTATAATG
    ACATATGAAC TCAAATTGCC ACCAAAAGCT TCCCTACTTC CAGGACCTCC AGAAGCATCT
    GAAGAATTTT ATATGACGAT TTATGAAGAC TCAGTTGCAA CGCATCTTAC AAAAATCCTC
    AATTCTGATG AACATGCAGT GGTCATATCT TCTGCCAAGA CGCTATGTGA AACTGTAAAG
    GACTTCGTTG CCAAAGTAGA AAAGACGTAT GACAAAACCT TGGAAAATGC CGTTGTAGCT
    GATGCCGTGG CCAGTAAATG TTCAGTCCTA AACGAGAAGC TCGAACAACT GCTGCAGGCT
    TTGCACACAG ATTCCCAGGC TGCGCCTGTT CTCCCTGGCC TCAGCCCTCT CATTGTGGAA
    GAAGATGCTG TGGAATCGTC CAGTGAAGAG TCCCTGGGTG AAAGCAAAGA GCAGCTTGGG
    GATGACGTTA CAAAACCTTC CTCCCAGAAA GCCGTCAAAC CAAGGGAAAT CATGTTGCGG
    GCAAATAGTT TAAAGAAAGC AGTGAGGCAA GTCATTGAGG AAGCCGGAAA AGTTATGGAT
    GACCCGACAG TTCACCCCTG TGAACCAGCT AATCAGTCCT CTGATTATGA CAGCACAGAA
    ACAGATGAAT CTAAGGAGGA AGCTAAAGAT GATGGTGCCA AAGAATCAAT AACTGTTAAA
    ACTGCACCTC GGTCTCCAGA TGCCCGGGCA AGTTATGGCC ATTCCCAAAC TGATTCTGTC
    CCTGGTCCAG CTGTGGCAGC CAGCAAAGAA AACCTCCCTG TGCTCAATAC CAGAATAATC
    TGCCCAGGTT TAAGAGCAGG ACTGGCTGCC TCAATTGCTG GGAGTTCGAT TATCAACAAA
    ATGTTACTGG CAAACATTGA TCCTTTTGGT GCCACGCCGT TTATTGACCC GGATCTAGAT
    TCCGTAGATG GATATTCAGA AAAATGTGTC ATGAACAATT ACTTTGGGAT TGGATTAGAT
    GCAAAAATTT CATTAGAATT TAATAATAAA AGAGAGGAGC ACCCTGAAAA ATGCAGGAGC
    CGAACTAAAA ACTTGATGTG GTATGGAGTC CTTGGAACCC GGGAGTTATT ACAGAGATCG
    TACAAGAATT TAGAACAAAG GGTTCAACTT GAGTGTGATG GGCAGTATAT TCCTCTTCCC
    AGCTTGCAAG GCATAGCCGT GTTGAACATT CCCAGCTATG CTGGAGGCAC TAACTTTTGG
    GGTGGAACTA AAGAGGATGA TATATTTGCT GCACCATCCT TTGATGACAA GATCCTGGAA
    GTTGTAGCAA TATTTGATAG CATGCAAATG GCAGTTTCAA GGGTCATTAA ACTGCAGCAT
    CATCGAATAG CCCAGTGCCG TACAGTGAAA ATCACTATAT TTGGTGACGA AGGAGTCCCA
    GTGCAAGTGG ATGGTGAAGC GTGGGTTCAG CCTCCAGGGA TTATCAAAAT TGTGCACAAA
    AACAGAGCAC AAATGCTAAC AAGGGACAGA GCCTTTGAGA GCACTCTGAA ATCTTGGGAA
    GATAAGCAGA AGTGTGATTC TGGTAAACCA GTTCTCCGAA CCCATTTGTA CATCCATCAC
    GCCATTGACT TGGCAACAGA AGAGGTGTCG CAGATGCAGC TATGCTCCCA GGCTGCAGAG
    GAGCTCATTA CTAGGATATG TGACGCAGCC ACAATTCACT GTCTTTTGGA GCAAGAACTG
    GCCCATGCTG TGAATGCCTG CTCCCATGCC CTGAATAAAG CCAACCCAAG GTGCCCGGAG
    AGTCTTACAA GAGACACTGC CACTGAAATA GCCATCAATG TGAAGGCGCT GTATAATGAA
    ACAGAATCTT TGCTAGTTGG CAGGGTTCCT TTGCAGCTGG AATCGCCACA TGAAGAGCGA
    GTATCCAATG CCTTACACTC TGTGGAGGTG GAATTACAGA AACTGACAGA GATTCCTTGG
    CTTTATTATA TCTTACACCC AAATGAGGAT GAGGAACCTC CTATGGATTG CACCAAAAGG
    AACAACAGAA GCACCGTATT TCGAATAGTG CCAAAGTTTA AAAAGGAAAA GGTTCAGAAG
    CAGAAGACAA GTTCACAGCC TGTTCAGAAA TGGGGCACAG AGGAAGTTGC TGCTTGGCTG
    GATCTGCTCA ATTTGGGAGA GTACAAAGAT ATCTTCATCC GTCATGACAT CAGAGGGGCT
    GAACTTTTGC ATCTGGAAAG GCGAGATCTT AAGGATCTGG GGATACCGAA AGTGGGTCAT
    GTGAAGCGAA TTCTCCAGGG AATTAAAGAG CTTGGAAGGA GCACTCCACA GTCGGAGGTG
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_821077.1
    CDS127..3789
    Translation

    Target ORF information:

    RefSeq Version NM_178009.5
    Organism Homo sapiens(human)
    Definition Homo sapiens diacylglycerol kinase eta (DGKH), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_178009.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCAGGGG CCGGAGGCCA GCACCACCCT CCGGGCGCCG CTGGAGGAGC GGCCGCCGGA 
    GCCGGCGCCG CGGTCACCTC CGCCGCTGCC TCGGCGGGGC CGGGAGAGGA TTCGTCTGAC
    AGCGAAGCGG AGCAAGAGGG ACCCCAGAAA CTGATCCGCA AAGTGTCTAC CTCGGGGCAG
    ATCCGGACCA AGACCAGTAT TAAAGAGGGA CAGCTATTGA AGCAAACCAG TTCTTTCCAA
    AGGTGGAAAA AGCGATACTT CAAACTTCGA GGCCGCACCC TTTACTATGC AAAGGACTCA
    AAGTCTCTGA TATTTGATGA AGTTGACCTC TCAGATGCTA GTGTAGCTGA AGCAAGCACG
    AAAAATGCTA ACAACAGCTT CACGATCATC ACTCCATTCA GAAGGCTAAT GCTGTGTGCT
    GAGAACAGAA AGGAGATGGA GGATTGGATC AGCTCACTGA AGTCTGTACA GACCAGAGAA
    CCCTACGAGG TGGCCCAGTT TAATGTGGAA CATTTCTCAG GGATGCACAA CTGGTACGCC
    TGCTCCCACG CCCGACCCAC CTTCTGTAAC GTGTGCAGAG AGAGTCTTTC TGGAGTCACC
    TCCCATGGCC TGTCCTGCGA AGTGTGTAAA TTCAAGGCTC ACAAAAGATG TGCAGTGAGA
    GCAACAAATA ACTGTAAATG GACTACCCTG GCCTCCATCG GGAAGGACAT TATAGAAGAT
    GAAGATGGCG TCGCGATGCC TCACCAGTGG CTTGAGGGCA ACCTGCCTGT AAGTGCCAAG
    TGTGCTGTCT GCGACAAAAC ATGTGGCAGT GTTCTCCGTC TACAGGATTG GAAATGCCTT
    TGGTGTAAGA CAATGGTACA CACTGCCTGC AAAGATTTAT ACCATCCAAT ATGTCCACTT
    GGTCAATGTA AAGTATCTAT CATACCTCCA ATTGCACTAA ACAGCACCGA TTCCGATGGT
    TTCTGTAGAG CAACATTTTC GTTCTGTGTT AGTCCTCTAT TGGTTTTTGT CAATTCTAAG
    AGTGGAGATA ATCAGGGAGT AAAGTTCCTC CGTCGCTTTA AACAGTTGCT AAATCCGGCT
    CAGGTGTTTG ATTTAATGAA TGGAGGTCCT CATTTAGGTT TAAGATTATT TCAGAAGTTT
    GACAATTTCC GGATTCTTGT TTGTGGAGGC GATGGAAGTG TAGGTTGGGT TTTGTCAGAA
    ATCGATAAGC TCAACTTGAA TAAACAGTGT CAGCTGGGAG TGTTGCCTTT GGGTACAGGA
    AATGACCTTG CCCGAGTTCT TGGCTGGGGA GGTTCATATG ACGATGACAC CCAACTTCCT
    CAGATCCTAG AGAAACTGGA ACGAGCCAGT ACCAAAATGT TGGACAGGTG GAGTATAATG
    ACATATGAAC TCAAATTGCC ACCAAAAGCT TCCCTACTTC CAGGACCTCC AGAAGCATCT
    GAAGAATTTT ATATGACGAT TTATGAAGAC TCAGTTGCAA CGCATCTTAC AAAAATCCTC
    AATTCTGATG AACATGCAGT GGTCATATCT TCTGCCAAGA CGCTATGTGA AACTGTAAAG
    GACTTCGTTG CCAAAGTAGA AAAGACGTAT GACAAAACCT TGGAAAATGC CGTTGTAGCT
    GATGCCGTGG CCAGTAAATG TTCAGTCCTA AACGAGAAGC TCGAACAACT GCTGCAGGCT
    TTGCACACAG ATTCCCAGGC TGCGCCTGTT CTCCCTGGCC TCAGCCCTCT CATTGTGGAA
    GAAGATGCTG TGGAATCGTC CAGTGAAGAG TCCCTGGGTG AAAGCAAAGA GCAGCTTGGG
    GATGACGTTA CAAAACCTTC CTCCCAGAAA GCCGTCAAAC CAAGGGAAAT CATGTTGCGG
    GCAAATAGTT TAAAGAAAGC AGTGAGGCAA GTCATTGAGG AAGCCGGAAA AGTTATGGAT
    GACCCGACAG TTCACCCCTG TGAACCAGCT AATCAGTCCT CTGATTATGA CAGCACAGAA
    ACAGATGAAT CTAAGGAGGA AGCTAAAGAT GATGGTGCCA AAGAATCAAT AACTGTTAAA
    ACTGCACCTC GGTCTCCAGA TGCCCGGGCA AGTTATGGCC ATTCCCAAAC TGATTCTGTC
    CCTGGTCCAG CTGTGGCAGC CAGCAAAGAA AACCTCCCTG TGCTCAATAC CAGAATAATC
    TGCCCAGGTT TAAGAGCAGG ACTGGCTGCC TCAATTGCTG GGAGTTCGAT TATCAACAAA
    ATGTTACTGG CAAACATTGA TCCTTTTGGT GCCACGCCGT TTATTGACCC GGATCTAGAT
    TCCGTAGATG GATATTCAGA AAAATGTGTC ATGAACAATT ACTTTGGGAT TGGATTAGAT
    GCAAAAATTT CATTAGAATT TAATAATAAA AGAGAGGAGC ACCCTGAAAA ATGCAGGAGC
    CGAACTAAAA ACTTGATGTG GTATGGAGTC CTTGGAACCC GGGAGTTATT ACAGAGATCG
    TACAAGAATT TAGAACAAAG GGTTCAACTT GAGTGTGATG GGCAGTATAT TCCTCTTCCC
    AGCTTGCAAG GCATAGCCGT GTTGAACATT CCCAGCTATG CTGGAGGCAC TAACTTTTGG
    GGTGGAACTA AAGAGGATGA TATATTTGCT GCACCATCCT TTGATGACAA GATCCTGGAA
    GTTGTAGCAA TATTTGATAG CATGCAAATG GCAGTTTCAA GGGTCATTAA ACTGCAGCAT
    CATCGAATAG CCCAGTGCCG TACAGTGAAA ATCACTATAT TTGGTGACGA AGGAGTCCCA
    GTGCAAGTGG ATGGTGAAGC GTGGGTTCAG CCTCCAGGGA TTATCAAAAT TGTGCACAAA
    AACAGAGCAC AAATGCTAAC AAGGGACAGA GCCTTTGAGA GCACTCTGAA ATCTTGGGAA
    GATAAGCAGA AGTGTGATTC TGGTAAACCA GTTCTCCGAA CCCATTTGTA CATCCATCAC
    GCCATTGACT TGGCAACAGA AGAGGTGTCG CAGATGCAGC TATGCTCCCA GGCTGCAGAG
    GAGCTCATTA CTAGGATATG TGACGCAGCC ACAATTCACT GTCTTTTGGA GCAAGAACTG
    GCCCATGCTG TGAATGCCTG CTCCCATGCC CTGAATAAAG CCAACCCAAG GTGCCCGGAG
    AGTCTTACAA GAGACACTGC CACTGAAATA GCCATCAATG TGAAGGCGCT GTATAATGAA
    ACAGAATCTT TGCTAGTTGG CAGGGTTCCT TTGCAGCTGG AATCGCCACA TGAAGAGCGA
    GTATCCAATG CCTTACACTC TGTGGAGGTG GAATTACAGA AACTGACAGA GATTCCTTGG
    CTTTATTATA TCTTACACCC AAATGAGGAT GAGGAACCTC CTATGGATTG CACCAAAAGG
    AACAACAGAA GCACCGTATT TCGAATAGTG CCAAAGTTTA AAAAGGAAAA GGTTCAGAAG
    CAGAAGACAA GTTCACAGCC TGTTCAGAAA TGGGGCACAG AGGAAGTTGC TGCTTGGCTG
    GATCTGCTCA ATTTGGGAGA GTACAAAGAT ATCTTCATCC GTCATGACAT CAGAGGGGCT
    GAACTTTTGC ATCTGGAAAG GCGAGATCTT AAGGATCTGG GGATACCGAA AGTGGGTCAT
    GTGAAGCGAA TTCTCCAGGG AATTAAAGAG CTTGGAAGGA GCACTCCACA GTCGGAGGTG
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu28742
    Clone ID Related Accession (Same CDS sequence) NM_001297429.1 , NM_001297429.2
    Accession Version NM_001297429.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2760bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-01-26
    Organism Homo sapiens(human)
    Product diacylglycerol kinase eta isoform 5
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK302727.1, AK302631.1, BC043292.2, AL139328.8, AL136527.9 and BM978856.1. On Jul 2, 2014 this sequence version replaced XM_005266271.1. Transcript Variant: This variant (6) differs in the 5' UTR, lacks a portion of the 5' coding region, two exons in the central coding region, and an in-frame exon in the 3' coding region, compared to variant 1. It initiates translation at an alternate downstream start codon. The encoded isoform (5) has a distinct N-terminus and is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK302631.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGTGGAACA TTTCTCAGGG ATGCACAACT GGTACGCCTG CTCCCACGCC CGACCCACCT 
    TCTGTAACGT GTGCAGAGAG AGTCTTTCTG GAGTCACCTC CCATGGCCTG TCCTGCGAAG
    GTACACACTG CCTGCAAAGA TTTATACCAT CCAATATGTC CACTTGGTCA ATGTAAAGTA
    TCTATCATAC CTCCAATTGC ACTAAACAGC ACCGATTCCG ATGGTTTCTG TAGAGCAACA
    TTTTCGTTCT GTGTTAGTCC TCTATTGGTT TTTGTCAATT CTAAGAGTGG AGATAATCAG
    GGAGTAAAGT TCCTCCGTCG CTTTAAACAG TTGCTAAATC CGGCTCAGGT GTTTGATTTA
    ATGAATGGAG GTCCTCATTT AGGTTTAAGA TTATTTCAGA AGTTTGACAA TTTCCGGATT
    CTTGTTTGTG GAGGCGATGG AAGTGTAGGT TGGGTTTTGT CAGAAATCGA TAAGCTCAAC
    TTGAATAAAC AGTGTCAGCT GGGAGTGTTG CCTTTGGGTA CAGGAAATGA CCTTGCCCGA
    GTTCTTGGCT GGGGAGGTTC ATATGACGAT GACACCCAAC TTCCTCAGAT CCTAGAGAAA
    CTGGAACGAG CCAGTACCAA AATGTTGGAC AGGTGGAGTA TAATGACATA TGAACTCAAA
    TTGCCACCAA AAGCTTCCCT ACTTCCAGGA CCTCCAGAAG CATCTGAAGA ATTTTATATG
    ACGATTTATG AAGACTCAGT TGCAACGCAT CTTACAAAAA TCCTCAATTC TGATGAACAT
    GCAGTGGTCA TATCTTCTGC CAAGACGCTA TGTGAAACTG TAAAGGACTT CGTTGCCAAA
    GTAGAAAAGA CGTATGACAA AACCTTGGAA AATGCCGTTG TAGCTGATGC CGTGGCCAGT
    AAATGTTCAG TCCTAAACGA GAAGCTCGAA CAACTGCTGC AGGCTTTGCA CACAGATTCC
    CAGGCTGCGC CTGTTCTCCC TGGCCTCAGC CCTCTCATTG TGGAAGAAGA TGCTGTGGAA
    TCGTCCAGTG AAGAGTCCCT GGGTGAAAGC AAAGAGCAGC TTGGGGATGA CGTTACAAAA
    CCTTCCTCCC AGAAAGCCGT CAAACCAAGG GAAATCATGT TGCGGGCAAA TAGTTTAAAG
    AAAGCAGTGA GGCAAGTCAT TGAGGAAGCC GGAAAAGTTA TGGATGACCC GACAGTTCAC
    CCCTGTGAAC CAGCTAATCA GTCCTCTGAT TATGACAGCA CAGAAACAGA TGAATCTAAG
    GAGGAAGCTA AAGATGATGG TGCCAAAGAA TCAATAACTG TTAAAACTGC ACCTCGGTCT
    CCAGATGCCC GGGCAAGTTA TGGCCATTCC CAAACTGATT CTGTCCCTGG TCCAGCTGTG
    GCAGCCAGCA AAGAAAACCT CCCTGTGCTC AATACCAGAA TAATCTGCCC AGGTTTAAGA
    GCAGGACTGG CTGCCTCAAT TGCTGGGAGT TCGATTATCA ACAAAATGTT ACTGGCAAAC
    ATTGATCCTT TTGGTGCCAC GCCGTTTATT GACCCGGATC TAGATTCCGT AGATGGATAT
    TCAGAAAAAT GTGTCATGAA CAATTACTTT GGGATTGGAT TAGATGCAAA AATTTCATTA
    GAATTTAATA ATAAAAGAGA GGAGCACCCT GAAAAATGCA GGAGCCGAAC TAAAAACTTG
    ATGTGGTATG GAGTCCTTGG AACCCGGGAG TTATTACAGA GATCGTACAA GAATTTAGAA
    CAAAGGGTTC AACTTGAGTG TGATGGGCAG TATATTCCTC TTCCCAGCTT GCAAGGCATA
    GCCGTGTTGA ACATTCCCAG CTATGCTGGA GGCACTAACT TTTGGGGTGG AACTAAAGAG
    GATGATATAT TTGCTGCACC ATCCTTTGAT GACAAGATCC TGGAAGTTGT AGCAATATTT
    GATAGCATGC AAATGGCAGT TTCAAGGGTC ATTAAACTGC AGCATCATCG AATAGCCCAG
    TGCCGTACAG TGAAAATCAC TATATTTGGT GACGAAGGAG TCCCAGTGCA AGTGGATGGT
    GAAGCGTGGG TTCAGCCTCC AGGGATTATC AAAATTGTGC ACAAAAACAG AGCACAAATG
    CTAACAAGGG ACAGAGCCTT TGAGAGCACT CTGAAATCTT GGGAAGATAA GCAGAAGTGT
    GATTCTGGTA AACCAGTTCT CCGAACCCAT TTGTACATCC ATCACGCCAT TGACTTGGCA
    ACAGAAGAGG TGTCGCAGAT GCAGCTATGC TCCCAGGCTG CAGAGGAGCT CATTACTAGG
    ATATGTGACG CAGCCACAAT TCACTGTCTT TTGGAGCAAG AACTGGCCCA TGCTGTGAAT
    GCCTGCTCCC ATGCCCTGAA TAAAGCCAAC CCAAGGTGCC CGGAGAGTCT TACAAGAGAC
    ACTGCCACTG AAATAGCCAT CAATGTGAAG GCGCTGTATA ATGAAACAGA ATCTTTGCTA
    GTTGGCAGGG TTCCTTTGCA GCTGGAATCG CCACATGAAG AGCGAGTATC CAATGCCTTA
    CACTCTGTGG AGGTGGAATT ACAGAAACTG ACAGAGATTC CTTGGCTTTA TTATATCTTA
    CACCCAAATG AGGATGAGGA ACCTCCTATG GATTGCACCA AAAGGAACAA CAGAAGCACC
    GTATTTCGAA TAGTGCCAAA GTTTAAAAAG GAAAAGGTTC AGAAGCAGAA GACAAGTTCA
    CAGCCTGGAT CTGGGGATAC CGAAAGTGGG TCATGTGAAG CGAATTCTCC AGGGAATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001284358.1
    CDS227..2986
    Translation

    Target ORF information:

    RefSeq Version NM_001297429.1
    Organism Homo sapiens(human)
    Definition Homo sapiens diacylglycerol kinase eta (DGKH), transcript variant 6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001297429.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGTGGAACA TTTCTCAGGG ATGCACAACT GGTACGCCTG CTCCCACGCC CGACCCACCT 
    TCTGTAACGT GTGCAGAGAG AGTCTTTCTG GAGTCACCTC CCATGGCCTG TCCTGCGAAG
    GTACACACTG CCTGCAAAGA TTTATACCAT CCAATATGTC CACTTGGTCA ATGTAAAGTA
    TCTATCATAC CTCCAATTGC ACTAAACAGC ACCGATTCCG ATGGTTTCTG TAGAGCAACA
    TTTTCGTTCT GTGTTAGTCC TCTATTGGTT TTTGTCAATT CTAAGAGTGG AGATAATCAG
    GGAGTAAAGT TCCTCCGTCG CTTTAAACAG TTGCTAAATC CGGCTCAGGT GTTTGATTTA
    ATGAATGGAG GTCCTCATTT AGGTTTAAGA TTATTTCAGA AGTTTGACAA TTTCCGGATT
    CTTGTTTGTG GAGGCGATGG AAGTGTAGGT TGGGTTTTGT CAGAAATCGA TAAGCTCAAC
    TTGAATAAAC AGTGTCAGCT GGGAGTGTTG CCTTTGGGTA CAGGAAATGA CCTTGCCCGA
    GTTCTTGGCT GGGGAGGTTC ATATGACGAT GACACCCAAC TTCCTCAGAT CCTAGAGAAA
    CTGGAACGAG CCAGTACCAA AATGTTGGAC AGGTGGAGTA TAATGACATA TGAACTCAAA
    TTGCCACCAA AAGCTTCCCT ACTTCCAGGA CCTCCAGAAG CATCTGAAGA ATTTTATATG
    ACGATTTATG AAGACTCAGT TGCAACGCAT CTTACAAAAA TCCTCAATTC TGATGAACAT
    GCAGTGGTCA TATCTTCTGC CAAGACGCTA TGTGAAACTG TAAAGGACTT CGTTGCCAAA
    GTAGAAAAGA CGTATGACAA AACCTTGGAA AATGCCGTTG TAGCTGATGC CGTGGCCAGT
    AAATGTTCAG TCCTAAACGA GAAGCTCGAA CAACTGCTGC AGGCTTTGCA CACAGATTCC
    CAGGCTGCGC CTGTTCTCCC TGGCCTCAGC CCTCTCATTG TGGAAGAAGA TGCTGTGGAA
    TCGTCCAGTG AAGAGTCCCT GGGTGAAAGC AAAGAGCAGC TTGGGGATGA CGTTACAAAA
    CCTTCCTCCC AGAAAGCCGT CAAACCAAGG GAAATCATGT TGCGGGCAAA TAGTTTAAAG
    AAAGCAGTGA GGCAAGTCAT TGAGGAAGCC GGAAAAGTTA TGGATGACCC GACAGTTCAC
    CCCTGTGAAC CAGCTAATCA GTCCTCTGAT TATGACAGCA CAGAAACAGA TGAATCTAAG
    GAGGAAGCTA AAGATGATGG TGCCAAAGAA TCAATAACTG TTAAAACTGC ACCTCGGTCT
    CCAGATGCCC GGGCAAGTTA TGGCCATTCC CAAACTGATT CTGTCCCTGG TCCAGCTGTG
    GCAGCCAGCA AAGAAAACCT CCCTGTGCTC AATACCAGAA TAATCTGCCC AGGTTTAAGA
    GCAGGACTGG CTGCCTCAAT TGCTGGGAGT TCGATTATCA ACAAAATGTT ACTGGCAAAC
    ATTGATCCTT TTGGTGCCAC GCCGTTTATT GACCCGGATC TAGATTCCGT AGATGGATAT
    TCAGAAAAAT GTGTCATGAA CAATTACTTT GGGATTGGAT TAGATGCAAA AATTTCATTA
    GAATTTAATA ATAAAAGAGA GGAGCACCCT GAAAAATGCA GGAGCCGAAC TAAAAACTTG
    ATGTGGTATG GAGTCCTTGG AACCCGGGAG TTATTACAGA GATCGTACAA GAATTTAGAA
    CAAAGGGTTC AACTTGAGTG TGATGGGCAG TATATTCCTC TTCCCAGCTT GCAAGGCATA
    GCCGTGTTGA ACATTCCCAG CTATGCTGGA GGCACTAACT TTTGGGGTGG AACTAAAGAG
    GATGATATAT TTGCTGCACC ATCCTTTGAT GACAAGATCC TGGAAGTTGT AGCAATATTT
    GATAGCATGC AAATGGCAGT TTCAAGGGTC ATTAAACTGC AGCATCATCG AATAGCCCAG
    TGCCGTACAG TGAAAATCAC TATATTTGGT GACGAAGGAG TCCCAGTGCA AGTGGATGGT
    GAAGCGTGGG TTCAGCCTCC AGGGATTATC AAAATTGTGC ACAAAAACAG AGCACAAATG
    CTAACAAGGG ACAGAGCCTT TGAGAGCACT CTGAAATCTT GGGAAGATAA GCAGAAGTGT
    GATTCTGGTA AACCAGTTCT CCGAACCCAT TTGTACATCC ATCACGCCAT TGACTTGGCA
    ACAGAAGAGG TGTCGCAGAT GCAGCTATGC TCCCAGGCTG CAGAGGAGCT CATTACTAGG
    ATATGTGACG CAGCCACAAT TCACTGTCTT TTGGAGCAAG AACTGGCCCA TGCTGTGAAT
    GCCTGCTCCC ATGCCCTGAA TAAAGCCAAC CCAAGGTGCC CGGAGAGTCT TACAAGAGAC
    ACTGCCACTG AAATAGCCAT CAATGTGAAG GCGCTGTATA ATGAAACAGA ATCTTTGCTA
    GTTGGCAGGG TTCCTTTGCA GCTGGAATCG CCACATGAAG AGCGAGTATC CAATGCCTTA
    CACTCTGTGG AGGTGGAATT ACAGAAACTG ACAGAGATTC CTTGGCTTTA TTATATCTTA
    CACCCAAATG AGGATGAGGA ACCTCCTATG GATTGCACCA AAAGGAACAA CAGAAGCACC
    GTATTTCGAA TAGTGCCAAA GTTTAAAAAG GAAAAGGTTC AGAAGCAGAA GACAAGTTCA
    CAGCCTGGAT CTGGGGATAC CGAAAGTGGG TCATGTGAAG CGAATTCTCC AGGGAATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu28742
    Clone ID Related Accession (Same CDS sequence) NM_001297429.1 , NM_001297429.2
    Accession Version NM_001297429.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2760bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-28
    Organism Homo sapiens(human)
    Product diacylglycerol kinase eta isoform 5
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK302727.1, AK302631.1, BC043292.2, AL139328.8, AL136527.9 and BM978856.1. On Jun 2, 2019 this sequence version replaced NM_001297429.1. Transcript Variant: This variant (6) differs in the 5' UTR, lacks a portion of the 5' coding region, two exons in the central coding region, and an in-frame exon in the 3' coding region, compared to variant 1. It initiates translation at an alternate downstream start codon. The encoded isoform (5) has a distinct N-terminus and is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK302631.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGTGGAACA TTTCTCAGGG ATGCACAACT GGTACGCCTG CTCCCACGCC CGACCCACCT 
    TCTGTAACGT GTGCAGAGAG AGTCTTTCTG GAGTCACCTC CCATGGCCTG TCCTGCGAAG
    GTACACACTG CCTGCAAAGA TTTATACCAT CCAATATGTC CACTTGGTCA ATGTAAAGTA
    TCTATCATAC CTCCAATTGC ACTAAACAGC ACCGATTCCG ATGGTTTCTG TAGAGCAACA
    TTTTCGTTCT GTGTTAGTCC TCTATTGGTT TTTGTCAATT CTAAGAGTGG AGATAATCAG
    GGAGTAAAGT TCCTCCGTCG CTTTAAACAG TTGCTAAATC CGGCTCAGGT GTTTGATTTA
    ATGAATGGAG GTCCTCATTT AGGTTTAAGA TTATTTCAGA AGTTTGACAA TTTCCGGATT
    CTTGTTTGTG GAGGCGATGG AAGTGTAGGT TGGGTTTTGT CAGAAATCGA TAAGCTCAAC
    TTGAATAAAC AGTGTCAGCT GGGAGTGTTG CCTTTGGGTA CAGGAAATGA CCTTGCCCGA
    GTTCTTGGCT GGGGAGGTTC ATATGACGAT GACACCCAAC TTCCTCAGAT CCTAGAGAAA
    CTGGAACGAG CCAGTACCAA AATGTTGGAC AGGTGGAGTA TAATGACATA TGAACTCAAA
    TTGCCACCAA AAGCTTCCCT ACTTCCAGGA CCTCCAGAAG CATCTGAAGA ATTTTATATG
    ACGATTTATG AAGACTCAGT TGCAACGCAT CTTACAAAAA TCCTCAATTC TGATGAACAT
    GCAGTGGTCA TATCTTCTGC CAAGACGCTA TGTGAAACTG TAAAGGACTT CGTTGCCAAA
    GTAGAAAAGA CGTATGACAA AACCTTGGAA AATGCCGTTG TAGCTGATGC CGTGGCCAGT
    AAATGTTCAG TCCTAAACGA GAAGCTCGAA CAACTGCTGC AGGCTTTGCA CACAGATTCC
    CAGGCTGCGC CTGTTCTCCC TGGCCTCAGC CCTCTCATTG TGGAAGAAGA TGCTGTGGAA
    TCGTCCAGTG AAGAGTCCCT GGGTGAAAGC AAAGAGCAGC TTGGGGATGA CGTTACAAAA
    CCTTCCTCCC AGAAAGCCGT CAAACCAAGG GAAATCATGT TGCGGGCAAA TAGTTTAAAG
    AAAGCAGTGA GGCAAGTCAT TGAGGAAGCC GGAAAAGTTA TGGATGACCC GACAGTTCAC
    CCCTGTGAAC CAGCTAATCA GTCCTCTGAT TATGACAGCA CAGAAACAGA TGAATCTAAG
    GAGGAAGCTA AAGATGATGG TGCCAAAGAA TCAATAACTG TTAAAACTGC ACCTCGGTCT
    CCAGATGCCC GGGCAAGTTA TGGCCATTCC CAAACTGATT CTGTCCCTGG TCCAGCTGTG
    GCAGCCAGCA AAGAAAACCT CCCTGTGCTC AATACCAGAA TAATCTGCCC AGGTTTAAGA
    GCAGGACTGG CTGCCTCAAT TGCTGGGAGT TCGATTATCA ACAAAATGTT ACTGGCAAAC
    ATTGATCCTT TTGGTGCCAC GCCGTTTATT GACCCGGATC TAGATTCCGT AGATGGATAT
    TCAGAAAAAT GTGTCATGAA CAATTACTTT GGGATTGGAT TAGATGCAAA AATTTCATTA
    GAATTTAATA ATAAAAGAGA GGAGCACCCT GAAAAATGCA GGAGCCGAAC TAAAAACTTG
    ATGTGGTATG GAGTCCTTGG AACCCGGGAG TTATTACAGA GATCGTACAA GAATTTAGAA
    CAAAGGGTTC AACTTGAGTG TGATGGGCAG TATATTCCTC TTCCCAGCTT GCAAGGCATA
    GCCGTGTTGA ACATTCCCAG CTATGCTGGA GGCACTAACT TTTGGGGTGG AACTAAAGAG
    GATGATATAT TTGCTGCACC ATCCTTTGAT GACAAGATCC TGGAAGTTGT AGCAATATTT
    GATAGCATGC AAATGGCAGT TTCAAGGGTC ATTAAACTGC AGCATCATCG AATAGCCCAG
    TGCCGTACAG TGAAAATCAC TATATTTGGT GACGAAGGAG TCCCAGTGCA AGTGGATGGT
    GAAGCGTGGG TTCAGCCTCC AGGGATTATC AAAATTGTGC ACAAAAACAG AGCACAAATG
    CTAACAAGGG ACAGAGCCTT TGAGAGCACT CTGAAATCTT GGGAAGATAA GCAGAAGTGT
    GATTCTGGTA AACCAGTTCT CCGAACCCAT TTGTACATCC ATCACGCCAT TGACTTGGCA
    ACAGAAGAGG TGTCGCAGAT GCAGCTATGC TCCCAGGCTG CAGAGGAGCT CATTACTAGG
    ATATGTGACG CAGCCACAAT TCACTGTCTT TTGGAGCAAG AACTGGCCCA TGCTGTGAAT
    GCCTGCTCCC ATGCCCTGAA TAAAGCCAAC CCAAGGTGCC CGGAGAGTCT TACAAGAGAC
    ACTGCCACTG AAATAGCCAT CAATGTGAAG GCGCTGTATA ATGAAACAGA ATCTTTGCTA
    GTTGGCAGGG TTCCTTTGCA GCTGGAATCG CCACATGAAG AGCGAGTATC CAATGCCTTA
    CACTCTGTGG AGGTGGAATT ACAGAAACTG ACAGAGATTC CTTGGCTTTA TTATATCTTA
    CACCCAAATG AGGATGAGGA ACCTCCTATG GATTGCACCA AAAGGAACAA CAGAAGCACC
    GTATTTCGAA TAGTGCCAAA GTTTAAAAAG GAAAAGGTTC AGAAGCAGAA GACAAGTTCA
    CAGCCTGGAT CTGGGGATAC CGAAAGTGGG TCATGTGAAG CGAATTCTCC AGGGAATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001284358.1
    CDS211..2970
    Translation

    Target ORF information:

    RefSeq Version NM_001297429.2
    Organism Homo sapiens(human)
    Definition Homo sapiens diacylglycerol kinase eta (DGKH), transcript variant 6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001297429.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGTGGAACA TTTCTCAGGG ATGCACAACT GGTACGCCTG CTCCCACGCC CGACCCACCT 
    TCTGTAACGT GTGCAGAGAG AGTCTTTCTG GAGTCACCTC CCATGGCCTG TCCTGCGAAG
    GTACACACTG CCTGCAAAGA TTTATACCAT CCAATATGTC CACTTGGTCA ATGTAAAGTA
    TCTATCATAC CTCCAATTGC ACTAAACAGC ACCGATTCCG ATGGTTTCTG TAGAGCAACA
    TTTTCGTTCT GTGTTAGTCC TCTATTGGTT TTTGTCAATT CTAAGAGTGG AGATAATCAG
    GGAGTAAAGT TCCTCCGTCG CTTTAAACAG TTGCTAAATC CGGCTCAGGT GTTTGATTTA
    ATGAATGGAG GTCCTCATTT AGGTTTAAGA TTATTTCAGA AGTTTGACAA TTTCCGGATT
    CTTGTTTGTG GAGGCGATGG AAGTGTAGGT TGGGTTTTGT CAGAAATCGA TAAGCTCAAC
    TTGAATAAAC AGTGTCAGCT GGGAGTGTTG CCTTTGGGTA CAGGAAATGA CCTTGCCCGA
    GTTCTTGGCT GGGGAGGTTC ATATGACGAT GACACCCAAC TTCCTCAGAT CCTAGAGAAA
    CTGGAACGAG CCAGTACCAA AATGTTGGAC AGGTGGAGTA TAATGACATA TGAACTCAAA
    TTGCCACCAA AAGCTTCCCT ACTTCCAGGA CCTCCAGAAG CATCTGAAGA ATTTTATATG
    ACGATTTATG AAGACTCAGT TGCAACGCAT CTTACAAAAA TCCTCAATTC TGATGAACAT
    GCAGTGGTCA TATCTTCTGC CAAGACGCTA TGTGAAACTG TAAAGGACTT CGTTGCCAAA
    GTAGAAAAGA CGTATGACAA AACCTTGGAA AATGCCGTTG TAGCTGATGC CGTGGCCAGT
    AAATGTTCAG TCCTAAACGA GAAGCTCGAA CAACTGCTGC AGGCTTTGCA CACAGATTCC
    CAGGCTGCGC CTGTTCTCCC TGGCCTCAGC CCTCTCATTG TGGAAGAAGA TGCTGTGGAA
    TCGTCCAGTG AAGAGTCCCT GGGTGAAAGC AAAGAGCAGC TTGGGGATGA CGTTACAAAA
    CCTTCCTCCC AGAAAGCCGT CAAACCAAGG GAAATCATGT TGCGGGCAAA TAGTTTAAAG
    AAAGCAGTGA GGCAAGTCAT TGAGGAAGCC GGAAAAGTTA TGGATGACCC GACAGTTCAC
    CCCTGTGAAC CAGCTAATCA GTCCTCTGAT TATGACAGCA CAGAAACAGA TGAATCTAAG
    GAGGAAGCTA AAGATGATGG TGCCAAAGAA TCAATAACTG TTAAAACTGC ACCTCGGTCT
    CCAGATGCCC GGGCAAGTTA TGGCCATTCC CAAACTGATT CTGTCCCTGG TCCAGCTGTG
    GCAGCCAGCA AAGAAAACCT CCCTGTGCTC AATACCAGAA TAATCTGCCC AGGTTTAAGA
    GCAGGACTGG CTGCCTCAAT TGCTGGGAGT TCGATTATCA ACAAAATGTT ACTGGCAAAC
    ATTGATCCTT TTGGTGCCAC GCCGTTTATT GACCCGGATC TAGATTCCGT AGATGGATAT
    TCAGAAAAAT GTGTCATGAA CAATTACTTT GGGATTGGAT TAGATGCAAA AATTTCATTA
    GAATTTAATA ATAAAAGAGA GGAGCACCCT GAAAAATGCA GGAGCCGAAC TAAAAACTTG
    ATGTGGTATG GAGTCCTTGG AACCCGGGAG TTATTACAGA GATCGTACAA GAATTTAGAA
    CAAAGGGTTC AACTTGAGTG TGATGGGCAG TATATTCCTC TTCCCAGCTT GCAAGGCATA
    GCCGTGTTGA ACATTCCCAG CTATGCTGGA GGCACTAACT TTTGGGGTGG AACTAAAGAG
    GATGATATAT TTGCTGCACC ATCCTTTGAT GACAAGATCC TGGAAGTTGT AGCAATATTT
    GATAGCATGC AAATGGCAGT TTCAAGGGTC ATTAAACTGC AGCATCATCG AATAGCCCAG
    TGCCGTACAG TGAAAATCAC TATATTTGGT GACGAAGGAG TCCCAGTGCA AGTGGATGGT
    GAAGCGTGGG TTCAGCCTCC AGGGATTATC AAAATTGTGC ACAAAAACAG AGCACAAATG
    CTAACAAGGG ACAGAGCCTT TGAGAGCACT CTGAAATCTT GGGAAGATAA GCAGAAGTGT
    GATTCTGGTA AACCAGTTCT CCGAACCCAT TTGTACATCC ATCACGCCAT TGACTTGGCA
    ACAGAAGAGG TGTCGCAGAT GCAGCTATGC TCCCAGGCTG CAGAGGAGCT CATTACTAGG
    ATATGTGACG CAGCCACAAT TCACTGTCTT TTGGAGCAAG AACTGGCCCA TGCTGTGAAT
    GCCTGCTCCC ATGCCCTGAA TAAAGCCAAC CCAAGGTGCC CGGAGAGTCT TACAAGAGAC
    ACTGCCACTG AAATAGCCAT CAATGTGAAG GCGCTGTATA ATGAAACAGA ATCTTTGCTA
    GTTGGCAGGG TTCCTTTGCA GCTGGAATCG CCACATGAAG AGCGAGTATC CAATGCCTTA
    CACTCTGTGG AGGTGGAATT ACAGAAACTG ACAGAGATTC CTTGGCTTTA TTATATCTTA
    CACCCAAATG AGGATGAGGA ACCTCCTATG GATTGCACCA AAAGGAACAA CAGAAGCACC
    GTATTTCGAA TAGTGCCAAA GTTTAAAAAG GAAAAGGTTC AGAAGCAGAA GACAAGTTCA
    CAGCCTGGAT CTGGGGATAC CGAAAGTGGG TCATGTGAAG CGAATTCTCC AGGGAATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu28740
    Clone ID Related Accession (Same CDS sequence) NM_001204505.2 , NM_001204505.3
    Accession Version NM_001204505.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3303bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-01-26
    Organism Homo sapiens(human)
    Product diacylglycerol kinase eta isoform 3
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK302727.1, BC043292.2, AB078967.1, AL136527.9 and BM978856.1. On Jul 2, 2014 this sequence version replaced NM_001204505.1. Transcript Variant: This variant (4) differs in the 5' UTR, lacks a portion of the 5' coding region, and contains two alternate exons in the 3' coding region, which results in a frameshift, compared to variant 1. It initiates translation at a downstream in-frame start codon. The encoded isoform (3) has a shorter N-terminus and a distinct C-terminus, and is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC043292.2 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1968968, SAMEA2148093 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGCTGTGTG CTGAGAACAG AAAGGAGATG GAGGATTGGA TCAGCTCACT GAAGTCTGTA 
    CAGACCAGAG AACCCTACGA GGTGGCCCAG TTTAATGTGG AACATTTCTC AGGGATGCAC
    AACTGGTACG CCTGCTCCCA CGCCCGACCC ACCTTCTGTA ACGTGTGCAG AGAGAGTCTT
    TCTGGAGTCA CCTCCCATGG CCTGTCCTGC GAAGTGTGTA AATTCAAGGC TCACAAAAGA
    TGTGCAGTGA GAGCAACAAA TAACTGTAAA TGGACTACCC TGGCCTCCAT CGGGAAGGAC
    ATTATAGAAG ATGAAGATGG CGTCGCGATG CCTCACCAGT GGCTTGAGGG CAACCTGCCT
    GTAAGTGCCA AGTGTGCTGT CTGCGACAAA ACATGTGGCA GTGTTCTCCG TCTACAGGAT
    TGGAAATGCC TTTGGTGTAA GACAATGGTA CACACTGCCT GCAAAGATTT ATACCATCCA
    ATATGTCCAC TTGGTCAATG TAAAGTATCT ATCATACCTC CAATTGCACT AAACAGCACC
    GATTCCGATG GTTTCTGTAG AGCAACATTT TCGTTCTGTG TTAGTCCTCT ATTGGTTTTT
    GTCAATTCTA AGAGTGGAGA TAATCAGGGA GTAAAGTTCC TCCGTCGCTT TAAACAGTTG
    CTAAATCCGG CTCAGGTGTT TGATTTAATG AATGGAGGTC CTCATTTAGG TTTAAGATTA
    TTTCAGAAGT TTGACAATTT CCGGATTCTT GTTTGTGGAG GCGATGGAAG TGTAGGTTGG
    GTTTTGTCAG AAATCGATAA GCTCAACTTG AATAAACAGT GTCAGCTGGG AGTGTTGCCT
    TTGGGTACAG GAAATGACCT TGCCCGAGTT CTTGGCTGGG GAGGTTCATA TGACGATGAC
    ACCCAACTTC CTCAGATCCT AGAGAAACTG GAACGAGCCA GTACCAAAAT GTTGGACAGG
    TGGAGTATAA TGACATATGA ACTCAAATTG CCACCAAAAG CTTCCCTACT TCCAGGACCT
    CCAGAAGCAT CTGAAGAATT TTATATGACG ATTTATGAAG ACTCAGTTGC AACGCATCTT
    ACAAAAATCC TCAATTCTGA TGAACATGCA GTGGTCATAT CTTCTGCCAA GACGCTATGT
    GAAACTGTAA AGGACTTCGT TGCCAAAGTA GAAAAGACGT ATGACAAAAC CTTGGAAAAT
    GCCGTTGTAG CTGATGCCGT GGCCAGTAAA TGTTCAGTCC TAAACGAGAA GCTCGAACAA
    CTGCTGCAGG CTTTGCACAC AGATTCCCAG GCTGCGCCTG TTCTCCCTGG CCTCAGCCCT
    CTCATTGTGG AAGAAGATGC TGTGGAATCG TCCAGTGAAG AGTCCCTGGG TGAAAGCAAA
    GAGCAGCTTG GGGATGACGT TACAAAACCT TCCTCCCAGA AAGCCGTCAA ACCAAGGGAA
    ATCATGTTGC GGGCAAATAG TTTAAAGAAA GCAGTGAGGC AAGTCATTGA GGAAGCCGGA
    AAAGTTATGG ATGACCCGAC AGTTCACCCC TGTGAACCAG CTAATCAGTC CTCTGATTAT
    GACAGCACAG AAACAGATGA ATCTAAGGAG GAAGCTAAAG ATGATGGTGC CAAAGAATCA
    ATAACTGTTA AAACTGCACC TCGGTCTCCA GATGCCCGGG CAAGTTATGG CCATTCCCAA
    ACTGATTCTG TCCCTGGTCC AGCTGTGGCA GCCAGCAAAG AAAACCTCCC TGTGCTCAAT
    ACCAGAATAA TCTGCCCAGG TTTAAGAGCA GGACTGGCTG CCTCAATTGC TGGGAGTTCG
    ATTATCAACA AAATGTTACT GGCAAACATT GATCCTTTTG GTGCCACGCC GTTTATTGAC
    CCGGATCTAG ATTCCGTAGA TGGATATTCA GAAAAATGTG TCATGAACAA TTACTTTGGG
    ATTGGATTAG ATGCAAAAAT TTCATTAGAA TTTAATAATA AAAGAGAGGA GCACCCTGAA
    AAATGCAGGA GCCGAACTAA AAACTTGATG TGGTATGGAG TCCTTGGAAC CCGGGAGTTA
    TTACAGAGAT CGTACAAGAA TTTAGAACAA AGGGTTCAAC TTGAGTGTGA TGGGCAGTAT
    ATTCCTCTTC CCAGCTTGCA AGGCATAGCC GTGTTGAACA TTCCCAGCTA TGCTGGAGGC
    ACTAACTTTT GGGGTGGAAC TAAAGAGGAT GATATATTTG CTGCACCATC CTTTGATGAC
    AAGATCCTGG AAGTTGTAGC AATATTTGAT AGCATGCAAA TGGCAGTTTC AAGGGTCATT
    AAACTGCAGC ATCATCGAAT AGCCCAGTGC CGTACAGTGA AAATCACTAT ATTTGGTGAC
    GAAGGAGTCC CAGTGCAAGT GGATGGTGAA GCGTGGGTTC AGCCTCCAGG GATTATCAAA
    ATTGTGCACA AAAACAGAGC ACAAATGCTA ACAAGGGACA GAGCCTTTGA GAGCACTCTG
    AAATCTTGGG AAGATAAGCA GAAGTGTGAT TCTGGTAAAC CAGTTCTCCG AACCCATTTG
    TACATCCATC ACGCCATTGA CTTGGCAACA GAAGAGGTGT CGCAGATGCA GCTATGCTCC
    CAGGCTGCAG AGGAGCTCAT TACTAGGATA TGTGACGCAG CCACAATTCA CTGTCTTTTG
    GAGCAAGAAC TGGCCCATGC TGTGAATGCC TGCTCCCATG CCCTGAATAA AGCCAACCCA
    AGGTGCCCGG AGAGTCTTAC AAGAGACACT GCCACTGAAA TAGCCATCAA TGTGAAGGCG
    CTGTATAATG AAACAGAATC TTTGCTAGTT GGCAGGGTTC CTTTGCAGCT GGAATCGCCA
    CATGAAGAGC GAGTATCCAA TGCCTTACAC TCTGTGGAGG TGGAATTACA GAAACTGACA
    GAGATTCCTT GGCTTTATTA TATCTTACAC CCAAATGAGG ATGAGGAACC TCCTATGGAT
    TGCACCAAAA GGAACAACAG AAGCACCGTA TTTCGAATAG TGCCAAAGTT TAAAAAGGAA
    AAGGTTCAGA AGCAGAAGAC AAGTTCACAG CCTGTTCAGA AATGGGGCAC AGAGGAAGTT
    GCTGCTTGGC TGGATCTGCT CAATTTGGGA GAGTACAAAG ATATCTTCAT CCGTCATGAC
    ATCAGAGGGG CTGAACTTTT GCATCTGGAA AGGCGAGATC TTAAGAACAC AGTAGGAGAA
    AAAAGAGACA CCAAAGAAAA TGGTAAACAT ATGGATCTGG GGATACCGAA AGTGGGTCAT
    GTGAAGCGAA TTCTCCAGGG AATTAAAGAG CTTGGAAGGA GCACTCCACA GTCGGAGGTG
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001191434.1
    CDS133..3435
    Translation

    Target ORF information:

    RefSeq Version NM_001204505.2
    Organism Homo sapiens(human)
    Definition Homo sapiens diacylglycerol kinase eta (DGKH), transcript variant 4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001204505.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGCTGTGTG CTGAGAACAG AAAGGAGATG GAGGATTGGA TCAGCTCACT GAAGTCTGTA 
    CAGACCAGAG AACCCTACGA GGTGGCCCAG TTTAATGTGG AACATTTCTC AGGGATGCAC
    AACTGGTACG CCTGCTCCCA CGCCCGACCC ACCTTCTGTA ACGTGTGCAG AGAGAGTCTT
    TCTGGAGTCA CCTCCCATGG CCTGTCCTGC GAAGTGTGTA AATTCAAGGC TCACAAAAGA
    TGTGCAGTGA GAGCAACAAA TAACTGTAAA TGGACTACCC TGGCCTCCAT CGGGAAGGAC
    ATTATAGAAG ATGAAGATGG CGTCGCGATG CCTCACCAGT GGCTTGAGGG CAACCTGCCT
    GTAAGTGCCA AGTGTGCTGT CTGCGACAAA ACATGTGGCA GTGTTCTCCG TCTACAGGAT
    TGGAAATGCC TTTGGTGTAA GACAATGGTA CACACTGCCT GCAAAGATTT ATACCATCCA
    ATATGTCCAC TTGGTCAATG TAAAGTATCT ATCATACCTC CAATTGCACT AAACAGCACC
    GATTCCGATG GTTTCTGTAG AGCAACATTT TCGTTCTGTG TTAGTCCTCT ATTGGTTTTT
    GTCAATTCTA AGAGTGGAGA TAATCAGGGA GTAAAGTTCC TCCGTCGCTT TAAACAGTTG
    CTAAATCCGG CTCAGGTGTT TGATTTAATG AATGGAGGTC CTCATTTAGG TTTAAGATTA
    TTTCAGAAGT TTGACAATTT CCGGATTCTT GTTTGTGGAG GCGATGGAAG TGTAGGTTGG
    GTTTTGTCAG AAATCGATAA GCTCAACTTG AATAAACAGT GTCAGCTGGG AGTGTTGCCT
    TTGGGTACAG GAAATGACCT TGCCCGAGTT CTTGGCTGGG GAGGTTCATA TGACGATGAC
    ACCCAACTTC CTCAGATCCT AGAGAAACTG GAACGAGCCA GTACCAAAAT GTTGGACAGG
    TGGAGTATAA TGACATATGA ACTCAAATTG CCACCAAAAG CTTCCCTACT TCCAGGACCT
    CCAGAAGCAT CTGAAGAATT TTATATGACG ATTTATGAAG ACTCAGTTGC AACGCATCTT
    ACAAAAATCC TCAATTCTGA TGAACATGCA GTGGTCATAT CTTCTGCCAA GACGCTATGT
    GAAACTGTAA AGGACTTCGT TGCCAAAGTA GAAAAGACGT ATGACAAAAC CTTGGAAAAT
    GCCGTTGTAG CTGATGCCGT GGCCAGTAAA TGTTCAGTCC TAAACGAGAA GCTCGAACAA
    CTGCTGCAGG CTTTGCACAC AGATTCCCAG GCTGCGCCTG TTCTCCCTGG CCTCAGCCCT
    CTCATTGTGG AAGAAGATGC TGTGGAATCG TCCAGTGAAG AGTCCCTGGG TGAAAGCAAA
    GAGCAGCTTG GGGATGACGT TACAAAACCT TCCTCCCAGA AAGCCGTCAA ACCAAGGGAA
    ATCATGTTGC GGGCAAATAG TTTAAAGAAA GCAGTGAGGC AAGTCATTGA GGAAGCCGGA
    AAAGTTATGG ATGACCCGAC AGTTCACCCC TGTGAACCAG CTAATCAGTC CTCTGATTAT
    GACAGCACAG AAACAGATGA ATCTAAGGAG GAAGCTAAAG ATGATGGTGC CAAAGAATCA
    ATAACTGTTA AAACTGCACC TCGGTCTCCA GATGCCCGGG CAAGTTATGG CCATTCCCAA
    ACTGATTCTG TCCCTGGTCC AGCTGTGGCA GCCAGCAAAG AAAACCTCCC TGTGCTCAAT
    ACCAGAATAA TCTGCCCAGG TTTAAGAGCA GGACTGGCTG CCTCAATTGC TGGGAGTTCG
    ATTATCAACA AAATGTTACT GGCAAACATT GATCCTTTTG GTGCCACGCC GTTTATTGAC
    CCGGATCTAG ATTCCGTAGA TGGATATTCA GAAAAATGTG TCATGAACAA TTACTTTGGG
    ATTGGATTAG ATGCAAAAAT TTCATTAGAA TTTAATAATA AAAGAGAGGA GCACCCTGAA
    AAATGCAGGA GCCGAACTAA AAACTTGATG TGGTATGGAG TCCTTGGAAC CCGGGAGTTA
    TTACAGAGAT CGTACAAGAA TTTAGAACAA AGGGTTCAAC TTGAGTGTGA TGGGCAGTAT
    ATTCCTCTTC CCAGCTTGCA AGGCATAGCC GTGTTGAACA TTCCCAGCTA TGCTGGAGGC
    ACTAACTTTT GGGGTGGAAC TAAAGAGGAT GATATATTTG CTGCACCATC CTTTGATGAC
    AAGATCCTGG AAGTTGTAGC AATATTTGAT AGCATGCAAA TGGCAGTTTC AAGGGTCATT
    AAACTGCAGC ATCATCGAAT AGCCCAGTGC CGTACAGTGA AAATCACTAT ATTTGGTGAC
    GAAGGAGTCC CAGTGCAAGT GGATGGTGAA GCGTGGGTTC AGCCTCCAGG GATTATCAAA
    ATTGTGCACA AAAACAGAGC ACAAATGCTA ACAAGGGACA GAGCCTTTGA GAGCACTCTG
    AAATCTTGGG AAGATAAGCA GAAGTGTGAT TCTGGTAAAC CAGTTCTCCG AACCCATTTG
    TACATCCATC ACGCCATTGA CTTGGCAACA GAAGAGGTGT CGCAGATGCA GCTATGCTCC
    CAGGCTGCAG AGGAGCTCAT TACTAGGATA TGTGACGCAG CCACAATTCA CTGTCTTTTG
    GAGCAAGAAC TGGCCCATGC TGTGAATGCC TGCTCCCATG CCCTGAATAA AGCCAACCCA
    AGGTGCCCGG AGAGTCTTAC AAGAGACACT GCCACTGAAA TAGCCATCAA TGTGAAGGCG
    CTGTATAATG AAACAGAATC TTTGCTAGTT GGCAGGGTTC CTTTGCAGCT GGAATCGCCA
    CATGAAGAGC GAGTATCCAA TGCCTTACAC TCTGTGGAGG TGGAATTACA GAAACTGACA
    GAGATTCCTT GGCTTTATTA TATCTTACAC CCAAATGAGG ATGAGGAACC TCCTATGGAT
    TGCACCAAAA GGAACAACAG AAGCACCGTA TTTCGAATAG TGCCAAAGTT TAAAAAGGAA
    AAGGTTCAGA AGCAGAAGAC AAGTTCACAG CCTGTTCAGA AATGGGGCAC AGAGGAAGTT
    GCTGCTTGGC TGGATCTGCT CAATTTGGGA GAGTACAAAG ATATCTTCAT CCGTCATGAC
    ATCAGAGGGG CTGAACTTTT GCATCTGGAA AGGCGAGATC TTAAGAACAC AGTAGGAGAA
    AAAAGAGACA CCAAAGAAAA TGGTAAACAT ATGGATCTGG GGATACCGAA AGTGGGTCAT
    GTGAAGCGAA TTCTCCAGGG AATTAAAGAG CTTGGAAGGA GCACTCCACA GTCGGAGGTG
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu28740
    Clone ID Related Accession (Same CDS sequence) NM_001204505.2 , NM_001204505.3
    Accession Version NM_001204505.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3303bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-28
    Organism Homo sapiens(human)
    Product diacylglycerol kinase eta isoform 3
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK302727.1, BC043292.2, AB078967.1, AL136527.9 and BM978856.1. On Jun 2, 2019 this sequence version replaced NM_001204505.2. Transcript Variant: This variant (4) differs in the 5' UTR, lacks a portion of the 5' coding region, and contains two alternate exons in the 3' coding region, which results in a frameshift, compared to variant 1. It initiates translation at a downstream in-frame start codon. The encoded isoform (3) has a shorter N-terminus and a distinct C-terminus, and is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC043292.2 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1968968, SAMEA2148093 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGCTGTGTG CTGAGAACAG AAAGGAGATG GAGGATTGGA TCAGCTCACT GAAGTCTGTA 
    CAGACCAGAG AACCCTACGA GGTGGCCCAG TTTAATGTGG AACATTTCTC AGGGATGCAC
    AACTGGTACG CCTGCTCCCA CGCCCGACCC ACCTTCTGTA ACGTGTGCAG AGAGAGTCTT
    TCTGGAGTCA CCTCCCATGG CCTGTCCTGC GAAGTGTGTA AATTCAAGGC TCACAAAAGA
    TGTGCAGTGA GAGCAACAAA TAACTGTAAA TGGACTACCC TGGCCTCCAT CGGGAAGGAC
    ATTATAGAAG ATGAAGATGG CGTCGCGATG CCTCACCAGT GGCTTGAGGG CAACCTGCCT
    GTAAGTGCCA AGTGTGCTGT CTGCGACAAA ACATGTGGCA GTGTTCTCCG TCTACAGGAT
    TGGAAATGCC TTTGGTGTAA GACAATGGTA CACACTGCCT GCAAAGATTT ATACCATCCA
    ATATGTCCAC TTGGTCAATG TAAAGTATCT ATCATACCTC CAATTGCACT AAACAGCACC
    GATTCCGATG GTTTCTGTAG AGCAACATTT TCGTTCTGTG TTAGTCCTCT ATTGGTTTTT
    GTCAATTCTA AGAGTGGAGA TAATCAGGGA GTAAAGTTCC TCCGTCGCTT TAAACAGTTG
    CTAAATCCGG CTCAGGTGTT TGATTTAATG AATGGAGGTC CTCATTTAGG TTTAAGATTA
    TTTCAGAAGT TTGACAATTT CCGGATTCTT GTTTGTGGAG GCGATGGAAG TGTAGGTTGG
    GTTTTGTCAG AAATCGATAA GCTCAACTTG AATAAACAGT GTCAGCTGGG AGTGTTGCCT
    TTGGGTACAG GAAATGACCT TGCCCGAGTT CTTGGCTGGG GAGGTTCATA TGACGATGAC
    ACCCAACTTC CTCAGATCCT AGAGAAACTG GAACGAGCCA GTACCAAAAT GTTGGACAGG
    TGGAGTATAA TGACATATGA ACTCAAATTG CCACCAAAAG CTTCCCTACT TCCAGGACCT
    CCAGAAGCAT CTGAAGAATT TTATATGACG ATTTATGAAG ACTCAGTTGC AACGCATCTT
    ACAAAAATCC TCAATTCTGA TGAACATGCA GTGGTCATAT CTTCTGCCAA GACGCTATGT
    GAAACTGTAA AGGACTTCGT TGCCAAAGTA GAAAAGACGT ATGACAAAAC CTTGGAAAAT
    GCCGTTGTAG CTGATGCCGT GGCCAGTAAA TGTTCAGTCC TAAACGAGAA GCTCGAACAA
    CTGCTGCAGG CTTTGCACAC AGATTCCCAG GCTGCGCCTG TTCTCCCTGG CCTCAGCCCT
    CTCATTGTGG AAGAAGATGC TGTGGAATCG TCCAGTGAAG AGTCCCTGGG TGAAAGCAAA
    GAGCAGCTTG GGGATGACGT TACAAAACCT TCCTCCCAGA AAGCCGTCAA ACCAAGGGAA
    ATCATGTTGC GGGCAAATAG TTTAAAGAAA GCAGTGAGGC AAGTCATTGA GGAAGCCGGA
    AAAGTTATGG ATGACCCGAC AGTTCACCCC TGTGAACCAG CTAATCAGTC CTCTGATTAT
    GACAGCACAG AAACAGATGA ATCTAAGGAG GAAGCTAAAG ATGATGGTGC CAAAGAATCA
    ATAACTGTTA AAACTGCACC TCGGTCTCCA GATGCCCGGG CAAGTTATGG CCATTCCCAA
    ACTGATTCTG TCCCTGGTCC AGCTGTGGCA GCCAGCAAAG AAAACCTCCC TGTGCTCAAT
    ACCAGAATAA TCTGCCCAGG TTTAAGAGCA GGACTGGCTG CCTCAATTGC TGGGAGTTCG
    ATTATCAACA AAATGTTACT GGCAAACATT GATCCTTTTG GTGCCACGCC GTTTATTGAC
    CCGGATCTAG ATTCCGTAGA TGGATATTCA GAAAAATGTG TCATGAACAA TTACTTTGGG
    ATTGGATTAG ATGCAAAAAT TTCATTAGAA TTTAATAATA AAAGAGAGGA GCACCCTGAA
    AAATGCAGGA GCCGAACTAA AAACTTGATG TGGTATGGAG TCCTTGGAAC CCGGGAGTTA
    TTACAGAGAT CGTACAAGAA TTTAGAACAA AGGGTTCAAC TTGAGTGTGA TGGGCAGTAT
    ATTCCTCTTC CCAGCTTGCA AGGCATAGCC GTGTTGAACA TTCCCAGCTA TGCTGGAGGC
    ACTAACTTTT GGGGTGGAAC TAAAGAGGAT GATATATTTG CTGCACCATC CTTTGATGAC
    AAGATCCTGG AAGTTGTAGC AATATTTGAT AGCATGCAAA TGGCAGTTTC AAGGGTCATT
    AAACTGCAGC ATCATCGAAT AGCCCAGTGC CGTACAGTGA AAATCACTAT ATTTGGTGAC
    GAAGGAGTCC CAGTGCAAGT GGATGGTGAA GCGTGGGTTC AGCCTCCAGG GATTATCAAA
    ATTGTGCACA AAAACAGAGC ACAAATGCTA ACAAGGGACA GAGCCTTTGA GAGCACTCTG
    AAATCTTGGG AAGATAAGCA GAAGTGTGAT TCTGGTAAAC CAGTTCTCCG AACCCATTTG
    TACATCCATC ACGCCATTGA CTTGGCAACA GAAGAGGTGT CGCAGATGCA GCTATGCTCC
    CAGGCTGCAG AGGAGCTCAT TACTAGGATA TGTGACGCAG CCACAATTCA CTGTCTTTTG
    GAGCAAGAAC TGGCCCATGC TGTGAATGCC TGCTCCCATG CCCTGAATAA AGCCAACCCA
    AGGTGCCCGG AGAGTCTTAC AAGAGACACT GCCACTGAAA TAGCCATCAA TGTGAAGGCG
    CTGTATAATG AAACAGAATC TTTGCTAGTT GGCAGGGTTC CTTTGCAGCT GGAATCGCCA
    CATGAAGAGC GAGTATCCAA TGCCTTACAC TCTGTGGAGG TGGAATTACA GAAACTGACA
    GAGATTCCTT GGCTTTATTA TATCTTACAC CCAAATGAGG ATGAGGAACC TCCTATGGAT
    TGCACCAAAA GGAACAACAG AAGCACCGTA TTTCGAATAG TGCCAAAGTT TAAAAAGGAA
    AAGGTTCAGA AGCAGAAGAC AAGTTCACAG CCTGTTCAGA AATGGGGCAC AGAGGAAGTT
    GCTGCTTGGC TGGATCTGCT CAATTTGGGA GAGTACAAAG ATATCTTCAT CCGTCATGAC
    ATCAGAGGGG CTGAACTTTT GCATCTGGAA AGGCGAGATC TTAAGAACAC AGTAGGAGAA
    AAAAGAGACA CCAAAGAAAA TGGTAAACAT ATGGATCTGG GGATACCGAA AGTGGGTCAT
    GTGAAGCGAA TTCTCCAGGG AATTAAAGAG CTTGGAAGGA GCACTCCACA GTCGGAGGTG
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001191434.1
    CDS117..3419
    Translation

    Target ORF information:

    RefSeq Version NM_001204505.3
    Organism Homo sapiens(human)
    Definition Homo sapiens diacylglycerol kinase eta (DGKH), transcript variant 4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001204505.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGCTGTGTG CTGAGAACAG AAAGGAGATG GAGGATTGGA TCAGCTCACT GAAGTCTGTA 
    CAGACCAGAG AACCCTACGA GGTGGCCCAG TTTAATGTGG AACATTTCTC AGGGATGCAC
    AACTGGTACG CCTGCTCCCA CGCCCGACCC ACCTTCTGTA ACGTGTGCAG AGAGAGTCTT
    TCTGGAGTCA CCTCCCATGG CCTGTCCTGC GAAGTGTGTA AATTCAAGGC TCACAAAAGA
    TGTGCAGTGA GAGCAACAAA TAACTGTAAA TGGACTACCC TGGCCTCCAT CGGGAAGGAC
    ATTATAGAAG ATGAAGATGG CGTCGCGATG CCTCACCAGT GGCTTGAGGG CAACCTGCCT
    GTAAGTGCCA AGTGTGCTGT CTGCGACAAA ACATGTGGCA GTGTTCTCCG TCTACAGGAT
    TGGAAATGCC TTTGGTGTAA GACAATGGTA CACACTGCCT GCAAAGATTT ATACCATCCA
    ATATGTCCAC TTGGTCAATG TAAAGTATCT ATCATACCTC CAATTGCACT AAACAGCACC
    GATTCCGATG GTTTCTGTAG AGCAACATTT TCGTTCTGTG TTAGTCCTCT ATTGGTTTTT
    GTCAATTCTA AGAGTGGAGA TAATCAGGGA GTAAAGTTCC TCCGTCGCTT TAAACAGTTG
    CTAAATCCGG CTCAGGTGTT TGATTTAATG AATGGAGGTC CTCATTTAGG TTTAAGATTA
    TTTCAGAAGT TTGACAATTT CCGGATTCTT GTTTGTGGAG GCGATGGAAG TGTAGGTTGG
    GTTTTGTCAG AAATCGATAA GCTCAACTTG AATAAACAGT GTCAGCTGGG AGTGTTGCCT
    TTGGGTACAG GAAATGACCT TGCCCGAGTT CTTGGCTGGG GAGGTTCATA TGACGATGAC
    ACCCAACTTC CTCAGATCCT AGAGAAACTG GAACGAGCCA GTACCAAAAT GTTGGACAGG
    TGGAGTATAA TGACATATGA ACTCAAATTG CCACCAAAAG CTTCCCTACT TCCAGGACCT
    CCAGAAGCAT CTGAAGAATT TTATATGACG ATTTATGAAG ACTCAGTTGC AACGCATCTT
    ACAAAAATCC TCAATTCTGA TGAACATGCA GTGGTCATAT CTTCTGCCAA GACGCTATGT
    GAAACTGTAA AGGACTTCGT TGCCAAAGTA GAAAAGACGT ATGACAAAAC CTTGGAAAAT
    GCCGTTGTAG CTGATGCCGT GGCCAGTAAA TGTTCAGTCC TAAACGAGAA GCTCGAACAA
    CTGCTGCAGG CTTTGCACAC AGATTCCCAG GCTGCGCCTG TTCTCCCTGG CCTCAGCCCT
    CTCATTGTGG AAGAAGATGC TGTGGAATCG TCCAGTGAAG AGTCCCTGGG TGAAAGCAAA
    GAGCAGCTTG GGGATGACGT TACAAAACCT TCCTCCCAGA AAGCCGTCAA ACCAAGGGAA
    ATCATGTTGC GGGCAAATAG TTTAAAGAAA GCAGTGAGGC AAGTCATTGA GGAAGCCGGA
    AAAGTTATGG ATGACCCGAC AGTTCACCCC TGTGAACCAG CTAATCAGTC CTCTGATTAT
    GACAGCACAG AAACAGATGA ATCTAAGGAG GAAGCTAAAG ATGATGGTGC CAAAGAATCA
    ATAACTGTTA AAACTGCACC TCGGTCTCCA GATGCCCGGG CAAGTTATGG CCATTCCCAA
    ACTGATTCTG TCCCTGGTCC AGCTGTGGCA GCCAGCAAAG AAAACCTCCC TGTGCTCAAT
    ACCAGAATAA TCTGCCCAGG TTTAAGAGCA GGACTGGCTG CCTCAATTGC TGGGAGTTCG
    ATTATCAACA AAATGTTACT GGCAAACATT GATCCTTTTG GTGCCACGCC GTTTATTGAC
    CCGGATCTAG ATTCCGTAGA TGGATATTCA GAAAAATGTG TCATGAACAA TTACTTTGGG
    ATTGGATTAG ATGCAAAAAT TTCATTAGAA TTTAATAATA AAAGAGAGGA GCACCCTGAA
    AAATGCAGGA GCCGAACTAA AAACTTGATG TGGTATGGAG TCCTTGGAAC CCGGGAGTTA
    TTACAGAGAT CGTACAAGAA TTTAGAACAA AGGGTTCAAC TTGAGTGTGA TGGGCAGTAT
    ATTCCTCTTC CCAGCTTGCA AGGCATAGCC GTGTTGAACA TTCCCAGCTA TGCTGGAGGC
    ACTAACTTTT GGGGTGGAAC TAAAGAGGAT GATATATTTG CTGCACCATC CTTTGATGAC
    AAGATCCTGG AAGTTGTAGC AATATTTGAT AGCATGCAAA TGGCAGTTTC AAGGGTCATT
    AAACTGCAGC ATCATCGAAT AGCCCAGTGC CGTACAGTGA AAATCACTAT ATTTGGTGAC
    GAAGGAGTCC CAGTGCAAGT GGATGGTGAA GCGTGGGTTC AGCCTCCAGG GATTATCAAA
    ATTGTGCACA AAAACAGAGC ACAAATGCTA ACAAGGGACA GAGCCTTTGA GAGCACTCTG
    AAATCTTGGG AAGATAAGCA GAAGTGTGAT TCTGGTAAAC CAGTTCTCCG AACCCATTTG
    TACATCCATC ACGCCATTGA CTTGGCAACA GAAGAGGTGT CGCAGATGCA GCTATGCTCC
    CAGGCTGCAG AGGAGCTCAT TACTAGGATA TGTGACGCAG CCACAATTCA CTGTCTTTTG
    GAGCAAGAAC TGGCCCATGC TGTGAATGCC TGCTCCCATG CCCTGAATAA AGCCAACCCA
    AGGTGCCCGG AGAGTCTTAC AAGAGACACT GCCACTGAAA TAGCCATCAA TGTGAAGGCG
    CTGTATAATG AAACAGAATC TTTGCTAGTT GGCAGGGTTC CTTTGCAGCT GGAATCGCCA
    CATGAAGAGC GAGTATCCAA TGCCTTACAC TCTGTGGAGG TGGAATTACA GAAACTGACA
    GAGATTCCTT GGCTTTATTA TATCTTACAC CCAAATGAGG ATGAGGAACC TCCTATGGAT
    TGCACCAAAA GGAACAACAG AAGCACCGTA TTTCGAATAG TGCCAAAGTT TAAAAAGGAA
    AAGGTTCAGA AGCAGAAGAC AAGTTCACAG CCTGTTCAGA AATGGGGCAC AGAGGAAGTT
    GCTGCTTGGC TGGATCTGCT CAATTTGGGA GAGTACAAAG ATATCTTCAT CCGTCATGAC
    ATCAGAGGGG CTGAACTTTT GCATCTGGAA AGGCGAGATC TTAAGAACAC AGTAGGAGAA
    AAAAGAGACA CCAAAGAAAA TGGTAAACAT ATGGATCTGG GGATACCGAA AGTGGGTCAT
    GTGAAGCGAA TTCTCCAGGG AATTAAAGAG CTTGGAAGGA GCACTCCACA GTCGGAGGTG
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.