DGKA cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	DGKA
Entrez Gene ID	1606
Full Name	diacylglycerol kinase alpha
Synonyms	DAGK,DAGK1,DGK-alpha
General protein information	Preferred Names diacylglycerol kinase alpha Names diacylglycerol kinase alpha 80 kDa diacylglycerol kinase DAG kinase alpha diacylglycerol kinase, alpha 80kDa diglyceride kinase alpha
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 12 Map Location: 12q13.2
Summary	The protein encoded by this gene belongs to the eukaryotic diacylglycerol kinase family. It acts as a modulator that competes with protein kinase C for the second messenger diacylglycerol in intracellular signaling pathways. It also plays an important role in the resynthesis of phosphatidylinositols and phosphorylating diacylglycerol to phosphatidic acid. Several transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Apr 2017].
Disorder MIM:	125855

mRNA and Protein(s)

mRNA	Protein	Name
XM_017018903.1	XP_016874392.1	diacylglycerol kinase alpha isoform X2
XM_017018904.1	XP_016874393.1	diacylglycerol kinase alpha isoform X2
XM_005268689.2	XP_005268746.1	diacylglycerol kinase alpha isoform X5
XM_011537995.2	XP_011536297.1	diacylglycerol kinase alpha isoform X5
NM_001351038.1	NP_001337967.1	diacylglycerol kinase alpha isoform d
NM_001351036.1	NP_001337965.1	diacylglycerol kinase alpha isoform c
NM_001351033.1	NP_001337962.1	diacylglycerol kinase alpha isoform a
NM_201445.1	NP_958853.1	diacylglycerol kinase alpha isoform a
NM_201554.1	NP_963848.1	diacylglycerol kinase alpha isoform a
NM_001345.4	NP_001336.2	diacylglycerol kinase alpha isoform a
NM_001351037.1	NP_001337966.1	diacylglycerol kinase alpha isoform c
NM_001351035.1	NP_001337964.1	diacylglycerol kinase alpha isoform b
XM_017018901.2	XP_016874390.1	diacylglycerol kinase alpha isoform X2
XM_017018907.1	XP_016874396.1	diacylglycerol kinase alpha isoform X2
XM_017018906.1	XP_016874395.1	diacylglycerol kinase alpha isoform X2
XM_005268690.2	XP_005268747.1	diacylglycerol kinase alpha isoform X6
XM_011537992.1	XP_011536294.1	diacylglycerol kinase alpha isoform X1
XM_017018908.1	XP_016874397.1	diacylglycerol kinase alpha isoform X3
XM_017018905.1	XP_016874394.1	diacylglycerol kinase alpha isoform X2
XM_017018906.2	XP_016874395.1	diacylglycerol kinase alpha isoform X2
XM_017018901.1	XP_016874390.1	diacylglycerol kinase alpha isoform X2
XM_011537991.1	XP_011536293.1	diacylglycerol kinase alpha isoform X1
XM_011537993.1	XP_011536295.1	diacylglycerol kinase alpha isoform X1
NM_001351039.1	NP_001337968.1	diacylglycerol kinase alpha isoform d
NM_001345.5	NP_001336.2	diacylglycerol kinase alpha isoform a
NM_201444.2	NP_958852.1	diacylglycerol kinase alpha isoform a
NM_001351034.1	NP_001337963.1	diacylglycerol kinase alpha isoform a
XM_017018900.1	XP_016874389.1	diacylglycerol kinase alpha isoform X1
XM_011537993.2	XP_011536295.1	diacylglycerol kinase alpha isoform X4
NM_001351040.1	NP_001337969.1	diacylglycerol kinase alpha isoform d
XM_017018902.1	XP_016874391.1	diacylglycerol kinase alpha isoform X2

Pathways from BioSystems

Homologies

Homo sapiens (human)	DGKA	NP_001336.2
	DGKA	XP_001112326.1
Canis lupus familiaris (dog)	DGKA	XP_005625534.1
Bos taurus (cattle)	DGKA	NP_001071328.1
Mus musculus (house mouse)	Dgka	NP_058091.2
Xenopus tropicalis (tropical clawed frog)	dgka	XP_002934577.1
Danio rerio (zebrafish)	dgka	XP_003199458.2
Rattus norvegicus (Norway rat)	Dgka	NP_542965.1
Pan troglodytes (chimpanzee)	DGKA	XP_001169946.1
Danio rerio (zebrafish)	dgkaa	NP_001038780.1

Gene Ontology
Bibliography

Related articles in PubMed

Nuclear Localization of Diacylglycerol Kinase Alpha in K562 Cells Is Involved in Cell Cycle Progression.
Poli A, Fiume R, Baldanzi G, Capello D, Ratti S, Gesi M, Manzoli L, Graziani A, Suh PG, Cocco L, Follo MY
Journal of cellular physiology232(9)2550-2557(2017 Sep)

Diacylglycerol kinases in cancer.
M?rida I, Torres-Ayuso P, ?vila-Flores A, Arranz-Nicol?s J, Andrada E, Tello-Lafoz M, Li?bana R, Arcos R
Advances in biological regulation6322-31(2017 Jan)

Decreased LIPF expression is correlated with DGKA and predicts poor outcome of gastric cancer.
Kong Y, Zheng Y, Jia Y, Li P, Wang Y
Oncology reports36(4)1852-60(2016 Oct)

Epigenetic regulation of diacylglycerol kinase alpha promotes radiation-induced fibrosis.
Weigel C, Veldwijk MR, Oakes CC, Seibold P, Slynko A, Liesenfeld DB, Rabionet M, Hanke SA, Wenz F, Sperk E, Benner A, R?sli C, Sandhoff R, Assenov Y, Plass C, Herskind C, Chang-Claude J, Schmezer P, Popanda O
Nature communications710893(2016 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

DGKalpha isoform is highly expressed in the nuclei of human erythroleukemia cell line K562, and its nuclear activity drives K562 cells through the G1/S transition during cell cycle progression.
Title: Nuclear Localization of Diacylglycerol Kinase Alpha in K562 Cells Is Involved in Cell Cycle Progression.

Diacylglycerol kinases alpha and zeta are up-regulated in cancer in cancer, and contribute towards tumor immune evasion and T cells clonal anergy. (Review)
Title: Diacylglycerol kinases in cancer.

LIPFDGKA might serve as a potential possible biomarkers for diagnosis of gastric cancer, and their downregulation may bring new perspective into the investigation of gastric cancer prognosis
Title: Decreased LIPF expression is correlated with DGKA and predicts poor outcome of gastric cancer.

Decreased DNA methylation at this enhancer enables recruitment of the profibrotic transcription factor early growth response 1 (EGR1) and facilitates radiation-induced DGKA transcription in cells from patients later developing fibrosis.
Title: Epigenetic regulation of diacylglycerol kinase alpha promotes radiation-induced fibrosis.

An abandoned compound that also inhibits serotonin receptors may have more translational potential as a DGKa inhibitor, but more potent and specific DGKa inhibitors are sorely needed
Title: Molecular Pathways: Targeting Diacylglycerol Kinase Alpha in Cancer.

The following DGKA gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DGKA cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu02866	NM_201445.1 Latest version!	Homo sapiens diacylglycerol kinase alpha (DGKA), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu02866	NM_201444.2 Latest version!	Homo sapiens diacylglycerol kinase alpha (DGKA), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu02866	NM_001345.5 Latest version!	Homo sapiens diacylglycerol kinase alpha (DGKA), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu02866	NM_001351034.1 Latest version!	Homo sapiens diacylglycerol kinase alpha (DGKA), transcript variant 6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu02866	NM_001351033.1 Latest version!	Homo sapiens diacylglycerol kinase alpha (DGKA), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu02866	NM_201554.1 Latest version!	Homo sapiens diacylglycerol kinase alpha (DGKA), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu02866	XM_011537993.2 Latest version!	Homo sapiens diacylglycerol kinase alpha (DGKA), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu02866	XM_011537992.1 Latest version!	Homo sapiens diacylglycerol kinase, alpha 80kDa (DGKA), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu02866	XM_011537991.1 Latest version!	Homo sapiens diacylglycerol kinase, alpha 80kDa (DGKA), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu102466	NM_001351040.1 Latest version!	Homo sapiens diacylglycerol kinase alpha (DGKA), transcript variant 12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OHu102466	NM_001351039.1 Latest version!	Homo sapiens diacylglycerol kinase alpha (DGKA), transcript variant 11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OHu102466	NM_001351038.1 Latest version!	Homo sapiens diacylglycerol kinase alpha (DGKA), transcript variant 10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OHu46356	XM_005268689.2 Latest version!	Homo sapiens diacylglycerol kinase alpha (DGKA), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu46356	XM_005268690.2 Latest version!	Homo sapiens diacylglycerol kinase alpha (DGKA), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu46356	XM_011537995.2 Latest version!	Homo sapiens diacylglycerol kinase alpha (DGKA), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu102467	NM_001351037.1 Latest version!	Homo sapiens diacylglycerol kinase alpha (DGKA), transcript variant 9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	10-12	$307.30 $439.00
OHu102467	NM_001351036.1 Latest version!	Homo sapiens diacylglycerol kinase alpha (DGKA), transcript variant 8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	10-12	$307.30 $439.00
OHu46355	NM_001351035.1 Latest version!	Homo sapiens diacylglycerol kinase alpha (DGKA), transcript variant 7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OHu83567	XM_017018900.1 Latest version!	Homo sapiens diacylglycerol kinase alpha (DGKA), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OHu83568	XM_017018906.2 Latest version!	Homo sapiens diacylglycerol kinase alpha (DGKA), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OHu83568	XM_017018905.1 Latest version!	Homo sapiens diacylglycerol kinase alpha (DGKA), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OHu83568	XM_017018907.1 Latest version!	Homo sapiens diacylglycerol kinase alpha (DGKA), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OHu83568	XM_017018901.2 Latest version!	Homo sapiens diacylglycerol kinase alpha (DGKA), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OHu83568	XM_017018902.1 Latest version!	Homo sapiens diacylglycerol kinase alpha (DGKA), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OHu83568	XM_017018903.1 Latest version!	Homo sapiens diacylglycerol kinase alpha (DGKA), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OHu83568	XM_017018904.1 Latest version!	Homo sapiens diacylglycerol kinase alpha (DGKA), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OHu83569	XM_017018908.1 Latest version!	Homo sapiens diacylglycerol kinase alpha (DGKA), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
View Old Accession Versions >>
OHu83568	XM_017018906.1	Homo sapiens diacylglycerol kinase alpha (DGKA), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OHu83568	XM_017018901.1	Homo sapiens diacylglycerol kinase alpha (DGKA), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OHu02866	NM_001345.4	Homo sapiens diacylglycerol kinase alpha (DGKA), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu02866	XM_011537993.1	Homo sapiens diacylglycerol kinase, alpha 80kDa (DGKA), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DGKA ( NM_001351033.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001337962.1 Homo sapiens diacylglycerol kinase alpha (DGKA), transcript variant 5, mRNA.

Price & Availability↑

CloneID	OHu02866
Clone ID Related Accession (Same CDS sequence)	NM_001351033.1 , NM_201445.1 , NM_201554.1 , NM_001345.4 , XM_011537992.1 , XM_011537991.1 , XM_011537993.1 , NM_001345.5 , NM_201444.2 , NM_001351034.1 , XM_011537993.2
Accession Version	NM_001351033.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2208bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-30
Organism	Homo sapiens(human)
Product	diacylglycerol kinase alpha isoform a
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC025162.48 and BC031870.2. Transcript Variant: This variant (5), as well as variants 1-4 and 6, encodes isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC031870.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2153031, SAMEA2156670 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCAAGG AGAGGGGCCT AATAAGCCCC AGTGATTTTG CCCAGCTGCA AAAATACATG 
GAATACTCCA CCAAAAAGGT CAGTGATGTC CTAAAGCTCT TCGAGGATGG CGAGATGGCT 
AAATATGTCC AAGGAGATGC CATTGGGTAC GAGGGATTCC AGCAATTCCT GAAAATCTAT 
CTCGAAGTGG ATAATGTTCC CAGACACCTA AGCCTGGCAC TGTTTCAATC CTTTGAGACT 
GGTCACTGCT TAAATGAGAC AAATGTGACA AAAGATGTGG TGTGTCTCAA TGATGTTTCC 
TGCTACTTTT CCCTTCTGGA GGGTGGTCGG CCAGAAGACA AGTTAGAATT CACCTTCAAG 
CTGTACGACA CGGACAGAAA TGGGATCCTG GACAGCTCAG AAGTGGACAA AATTATCCTA 
CAGATGATGC GAGTGGCTGA ATACCTGGAT TGGGATGTGT CTGAGCTGAG GCCGATTCTT 
CAGGAGATGA TGAAAGAGAT TGACTATGAT GGCAGTGGCT CTGTCTCTCA AGCTGAGTGG 
GTCCGGGCTG GGGCCACCAC CGTGCCACTG CTAGTGCTGC TGGGTCTGGA GATGACTCTG 
AAGGACGACG GACAGCACAT GTGGAGGCCC AAGAGGTTCC CCAGACCAGT CTACTGCAAT 
CTGTGCGAGT CAAGCATTGG TCTTGGCAAA CAGGGACTGA GCTGTAACCT CTGTAAGTAC 
ACTGTTCACG ACCAGTGTGC CATGAAAGCC CTGCCTTGTG AAGTCAGCAC CTATGCCAAG 
TCTCGGAAGG ACATTGGTGT CCAATCACAT GTGTGGGTGC GAGGAGGCTG TGAGTCCGGG 
CGCTGCGACC GCTGTCAGAA AAAGATCCGG ATCTACCACA GTCTGACCGG GCTGCATTGT 
GTATGGTGCC ACCTAGAGAT CCACGATGAC TGCCTGCAAG CGGTGGGCCA TGAGTGTGAC 
TGTGGGCTGC TCCGGGATCA CATCCTGCCT CCATCTTCCA TCTATCCCAG TGTCCTGGCC 
TCTGGACCGG ATCGTAAAAA TAGCAAAACA AGCCAGAAGA CCATGGATGA TTTAAATTTG 
AGCACCTCTG AGGCTCTGCG GATTGACCCT GTTCCTAACA CCCACCCACT TCTCGTCTTT 
GTCAATCCTA AGAGTGGCGG GAAGCAGGGG CAAAGGGTGC TCTGGAAGTT CCAGTATATA 
TTAAACCCTC GACAGGTGTT CAACCTCCTA AAGGATGGTC CTGAGATAGG GCTCCGATTA 
TTCAAGGATG TTCCTGATAG CCGGATTTTG GTGTGTGGTG GAGACGGCAC AGTAGGCTGG 
ATTCTAGAGA CCATTGACAA AGCTAACTTG CCAGTTTTGC CTCCTGTTGC TGTGTTGCCC 
CTGGGTACTG GAAATGATCT GGCTCGATGC CTAAGATGGG GAGGAGGTTA TGAAGGACAG 
AATCTGGCAA AGATCCTCAA GGATTTAGAG ATGAGTAAAG TGGTACATAT GGATCGATGG 
TCTGTGGAGG TGATACCTCA ACAAACTGAA GAAAAAAGTG ACCCAGTCCC CTTTCAAATC 
ATCAATAACT ACTTCTCTAT TGGCGTGGAT GCCTCTATTG CTCATCGATT CCACATCATG 
CGAGAGAAAT ATCCGGAGAA GTTCAACAGC AGAATGAAGA ACAAGCTATG GTACTTCGAA 
TTTGCCACAT CTGAATCCAT CTTCTCAACA TGCAAAAAGC TGGAGGAGTC TTTGACAGTT 
GAGATCTGTG GGAAACCGCT GGATCTGAGC AACCTGTCCC TAGAAGGCAT CGCAGTGCTA 
AACATCCCTA GCATGCATGG TGGCTCCAAC CTCTGGGGTG ATACCAGGAG ACCCCATGGG 
GATATCTATG GGATCAACCA GGCCTTAGGT GCTACAGCTA AAGTCATCAC CGACCCTGAT 
ATCCTGAAAA CCTGTGTACC AGACCTAAGT GACAAGAGAC TGGAAGTGGT TGGGCTGGAG 
GGTGCAATTG AGATGGGCCA AATCTATACC AAGCTCAAGA ATGCTGGACG TCGGCTGGCC 
AAGTGCTCTG AGATCACCTT CCACACCACA AAAACCCTTC CCATGCAAAT TGACGGAGAA 
CCCTGGATGC AGACGCCCTG TACAATCAAG ATCACCCACA AGAACCAGAT GCCCATGCTC 
ATGGGCCCAC CCCCCCGCTC CACCAATTTC TTTGGCTTCT TGAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001337962.1
CDS	235..2442
Translation	MAKERGLISPSDFAQLQKYMEYSTKKVSDVLKLFEDGEMAKYVQGDAIGYEGFQQFLKIYLEVDNVPRHLSLALFQSFETGHCLNETNVTKDVVCLNDVSCYFSLLEGGRPEDKLEFTFKLYDTDRNGILDSSEVDKIILQMMRVAEYLDWDVSELRPILQEMMKEIDYDGSGSVSQAEWVRAGATTVPLLVLLGLEMTLKDDGQHMWRPKRFPRPVYCNLCESSIGLGKQGLSCNLCKYTVHDQCAMKALPCEVSTYAKSRKDIGVQSHVWVRGGCESGRCDRCQKKIRIYHSLTGLHCVWCHLEIHDDCLQAVGHECDCGLLRDHILPPSSIYPSVLASGPDRKNSKTSQKTMDDLNLSTSEALRIDPVPNTHPLLVFVNPKSGGKQGQRVLWKFQYILNPRQVFNLLKDGPEIGLRLFKDVPDSRILVCGGDGTVGWILETIDKANLPVLPPVAVLPLGTGNDLARCLRWGGGYEGQNLAKILKDLEMSKVVHMDRWSVEVIPQQTEEKSDPVPFQIINNYFSIGVDASIAHRFHIMREKYPEKFNSRMKNKLWYFEFATSESIFSTCKKLEESLTVEICGKPLDLSNLSLEGIAVLNIPSMHGGSNLWGDTRRPHGDIYGINQALGATAKVITDPDILKTCVPDLSDKRLEVVGLEGAIEMGQIYTKLKNAGRRLAKCSEITFHTTKTLPMQIDGEPWMQTPCTIKITHKNQMPMLMGPPPRSTNFFGFLS

Target ORF information:

RefSeq Version	NM_001351033.1
Organism	Homo sapiens(human)
Definition	Homo sapiens diacylglycerol kinase alpha (DGKA), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001351033.1

ORF Insert Sequence:

ATGGCCAAGG AGAGGGGCCT AATAAGCCCC AGTGATTTTG CCCAGCTGCA AAAATACATG 
GAATACTCCA CCAAAAAGGT CAGTGATGTC CTAAAGCTCT TCGAGGATGG CGAGATGGCT 
AAATATGTCC AAGGAGATGC CATTGGGTAC GAGGGATTCC AGCAATTCCT GAAAATCTAT 
CTCGAAGTGG ATAATGTTCC CAGACACCTA AGCCTGGCAC TGTTTCAATC CTTTGAGACT 
GGTCACTGCT TAAATGAGAC AAATGTGACA AAAGATGTGG TGTGTCTCAA TGATGTTTCC 
TGCTACTTTT CCCTTCTGGA GGGTGGTCGG CCAGAAGACA AGTTAGAATT CACCTTCAAG 
CTGTACGACA CGGACAGAAA TGGGATCCTG GACAGCTCAG AAGTGGACAA AATTATCCTA 
CAGATGATGC GAGTGGCTGA ATACCTGGAT TGGGATGTGT CTGAGCTGAG GCCGATTCTT 
CAGGAGATGA TGAAAGAGAT TGACTATGAT GGCAGTGGCT CTGTCTCTCA AGCTGAGTGG 
GTCCGGGCTG GGGCCACCAC CGTGCCACTG CTAGTGCTGC TGGGTCTGGA GATGACTCTG 
AAGGACGACG GACAGCACAT GTGGAGGCCC AAGAGGTTCC CCAGACCAGT CTACTGCAAT 
CTGTGCGAGT CAAGCATTGG TCTTGGCAAA CAGGGACTGA GCTGTAACCT CTGTAAGTAC 
ACTGTTCACG ACCAGTGTGC CATGAAAGCC CTGCCTTGTG AAGTCAGCAC CTATGCCAAG 
TCTCGGAAGG ACATTGGTGT CCAATCACAT GTGTGGGTGC GAGGAGGCTG TGAGTCCGGG 
CGCTGCGACC GCTGTCAGAA AAAGATCCGG ATCTACCACA GTCTGACCGG GCTGCATTGT 
GTATGGTGCC ACCTAGAGAT CCACGATGAC TGCCTGCAAG CGGTGGGCCA TGAGTGTGAC 
TGTGGGCTGC TCCGGGATCA CATCCTGCCT CCATCTTCCA TCTATCCCAG TGTCCTGGCC 
TCTGGACCGG ATCGTAAAAA TAGCAAAACA AGCCAGAAGA CCATGGATGA TTTAAATTTG 
AGCACCTCTG AGGCTCTGCG GATTGACCCT GTTCCTAACA CCCACCCACT TCTCGTCTTT 
GTCAATCCTA AGAGTGGCGG GAAGCAGGGG CAAAGGGTGC TCTGGAAGTT CCAGTATATA 
TTAAACCCTC GACAGGTGTT CAACCTCCTA AAGGATGGTC CTGAGATAGG GCTCCGATTA 
TTCAAGGATG TTCCTGATAG CCGGATTTTG GTGTGTGGTG GAGACGGCAC AGTAGGCTGG 
ATTCTAGAGA CCATTGACAA AGCTAACTTG CCAGTTTTGC CTCCTGTTGC TGTGTTGCCC 
CTGGGTACTG GAAATGATCT GGCTCGATGC CTAAGATGGG GAGGAGGTTA TGAAGGACAG 
AATCTGGCAA AGATCCTCAA GGATTTAGAG ATGAGTAAAG TGGTACATAT GGATCGATGG 
TCTGTGGAGG TGATACCTCA ACAAACTGAA GAAAAAAGTG ACCCAGTCCC CTTTCAAATC 
ATCAATAACT ACTTCTCTAT TGGCGTGGAT GCCTCTATTG CTCATCGATT CCACATCATG 
CGAGAGAAAT ATCCGGAGAA GTTCAACAGC AGAATGAAGA ACAAGCTATG GTACTTCGAA 
TTTGCCACAT CTGAATCCAT CTTCTCAACA TGCAAAAAGC TGGAGGAGTC TTTGACAGTT 
GAGATCTGTG GGAAACCGCT GGATCTGAGC AACCTGTCCC TAGAAGGCAT CGCAGTGCTA 
AACATCCCTA GCATGCATGG TGGCTCCAAC CTCTGGGGTG ATACCAGGAG ACCCCATGGG 
GATATCTATG GGATCAACCA GGCCTTAGGT GCTACAGCTA AAGTCATCAC CGACCCTGAT 
ATCCTGAAAA CCTGTGTACC AGACCTAAGT GACAAGAGAC TGGAAGTGGT TGGGCTGGAG 
GGTGCAATTG AGATGGGCCA AATCTATACC AAGCTCAAGA ATGCTGGACG TCGGCTGGCC 
AAGTGCTCTG AGATCACCTT CCACACCACA AAAACCCTTC CCATGCAAAT TGACGGAGAA 
CCCTGGATGC AGACGCCCTG TACAATCAAG ATCACCCACA AGAACCAGAT GCCCATGCTC 
ATGGGCCCAC CCCCCCGCTC CACCAATTTC TTTGGCTTCT TGAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DGKA ( NM_201445.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_958853.1 Homo sapiens diacylglycerol kinase alpha (DGKA), transcript variant 2, mRNA.

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CloneID	OHu02866
Clone ID Related Accession (Same CDS sequence)	NM_001351033.1 , NM_201445.1 , NM_201554.1 , NM_001345.4 , XM_011537992.1 , XM_011537991.1 , XM_011537993.1 , NM_001345.5 , NM_201444.2 , NM_001351034.1 , XM_011537993.2
Accession Version	NM_201445.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2208bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-30
Organism	Homo sapiens(human)
Product	diacylglycerol kinase alpha isoform a
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BF448612.1, BC023523.2, BC031870.2 and AF064768.1. Transcript Variant: This variant (2), as well as variants 1 and 3-6, encodes isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC023523.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968832 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCAAGG AGAGGGGCCT AATAAGCCCC AGTGATTTTG CCCAGCTGCA AAAATACATG 
GAATACTCCA CCAAAAAGGT CAGTGATGTC CTAAAGCTCT TCGAGGATGG CGAGATGGCT 
AAATATGTCC AAGGAGATGC CATTGGGTAC GAGGGATTCC AGCAATTCCT GAAAATCTAT 
CTCGAAGTGG ATAATGTTCC CAGACACCTA AGCCTGGCAC TGTTTCAATC CTTTGAGACT 
GGTCACTGCT TAAATGAGAC AAATGTGACA AAAGATGTGG TGTGTCTCAA TGATGTTTCC 
TGCTACTTTT CCCTTCTGGA GGGTGGTCGG CCAGAAGACA AGTTAGAATT CACCTTCAAG 
CTGTACGACA CGGACAGAAA TGGGATCCTG GACAGCTCAG AAGTGGACAA AATTATCCTA 
CAGATGATGC GAGTGGCTGA ATACCTGGAT TGGGATGTGT CTGAGCTGAG GCCGATTCTT 
CAGGAGATGA TGAAAGAGAT TGACTATGAT GGCAGTGGCT CTGTCTCTCA AGCTGAGTGG 
GTCCGGGCTG GGGCCACCAC CGTGCCACTG CTAGTGCTGC TGGGTCTGGA GATGACTCTG 
AAGGACGACG GACAGCACAT GTGGAGGCCC AAGAGGTTCC CCAGACCAGT CTACTGCAAT 
CTGTGCGAGT CAAGCATTGG TCTTGGCAAA CAGGGACTGA GCTGTAACCT CTGTAAGTAC 
ACTGTTCACG ACCAGTGTGC CATGAAAGCC CTGCCTTGTG AAGTCAGCAC CTATGCCAAG 
TCTCGGAAGG ACATTGGTGT CCAATCACAT GTGTGGGTGC GAGGAGGCTG TGAGTCCGGG 
CGCTGCGACC GCTGTCAGAA AAAGATCCGG ATCTACCACA GTCTGACCGG GCTGCATTGT 
GTATGGTGCC ACCTAGAGAT CCACGATGAC TGCCTGCAAG CGGTGGGCCA TGAGTGTGAC 
TGTGGGCTGC TCCGGGATCA CATCCTGCCT CCATCTTCCA TCTATCCCAG TGTCCTGGCC 
TCTGGACCGG ATCGTAAAAA TAGCAAAACA AGCCAGAAGA CCATGGATGA TTTAAATTTG 
AGCACCTCTG AGGCTCTGCG GATTGACCCT GTTCCTAACA CCCACCCACT TCTCGTCTTT 
GTCAATCCTA AGAGTGGCGG GAAGCAGGGG CAAAGGGTGC TCTGGAAGTT CCAGTATATA 
TTAAACCCTC GACAGGTGTT CAACCTCCTA AAGGATGGTC CTGAGATAGG GCTCCGATTA 
TTCAAGGATG TTCCTGATAG CCGGATTTTG GTGTGTGGTG GAGACGGCAC AGTAGGCTGG 
ATTCTAGAGA CCATTGACAA AGCTAACTTG CCAGTTTTGC CTCCTGTTGC TGTGTTGCCC 
CTGGGTACTG GAAATGATCT GGCTCGATGC CTAAGATGGG GAGGAGGTTA TGAAGGACAG 
AATCTGGCAA AGATCCTCAA GGATTTAGAG ATGAGTAAAG TGGTACATAT GGATCGATGG 
TCTGTGGAGG TGATACCTCA ACAAACTGAA GAAAAAAGTG ACCCAGTCCC CTTTCAAATC 
ATCAATAACT ACTTCTCTAT TGGCGTGGAT GCCTCTATTG CTCATCGATT CCACATCATG 
CGAGAGAAAT ATCCGGAGAA GTTCAACAGC AGAATGAAGA ACAAGCTATG GTACTTCGAA 
TTTGCCACAT CTGAATCCAT CTTCTCAACA TGCAAAAAGC TGGAGGAGTC TTTGACAGTT 
GAGATCTGTG GGAAACCGCT GGATCTGAGC AACCTGTCCC TAGAAGGCAT CGCAGTGCTA 
AACATCCCTA GCATGCATGG TGGCTCCAAC CTCTGGGGTG ATACCAGGAG ACCCCATGGG 
GATATCTATG GGATCAACCA GGCCTTAGGT GCTACAGCTA AAGTCATCAC CGACCCTGAT 
ATCCTGAAAA CCTGTGTACC AGACCTAAGT GACAAGAGAC TGGAAGTGGT TGGGCTGGAG 
GGTGCAATTG AGATGGGCCA AATCTATACC AAGCTCAAGA ATGCTGGACG TCGGCTGGCC 
AAGTGCTCTG AGATCACCTT CCACACCACA AAAACCCTTC CCATGCAAAT TGACGGAGAA 
CCCTGGATGC AGACGCCCTG TACAATCAAG ATCACCCACA AGAACCAGAT GCCCATGCTC 
ATGGGCCCAC CCCCCCGCTC CACCAATTTC TTTGGCTTCT TGAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_958853.1
CDS	278..2485
Translation	MAKERGLISPSDFAQLQKYMEYSTKKVSDVLKLFEDGEMAKYVQGDAIGYEGFQQFLKIYLEVDNVPRHLSLALFQSFETGHCLNETNVTKDVVCLNDVSCYFSLLEGGRPEDKLEFTFKLYDTDRNGILDSSEVDKIILQMMRVAEYLDWDVSELRPILQEMMKEIDYDGSGSVSQAEWVRAGATTVPLLVLLGLEMTLKDDGQHMWRPKRFPRPVYCNLCESSIGLGKQGLSCNLCKYTVHDQCAMKALPCEVSTYAKSRKDIGVQSHVWVRGGCESGRCDRCQKKIRIYHSLTGLHCVWCHLEIHDDCLQAVGHECDCGLLRDHILPPSSIYPSVLASGPDRKNSKTSQKTMDDLNLSTSEALRIDPVPNTHPLLVFVNPKSGGKQGQRVLWKFQYILNPRQVFNLLKDGPEIGLRLFKDVPDSRILVCGGDGTVGWILETIDKANLPVLPPVAVLPLGTGNDLARCLRWGGGYEGQNLAKILKDLEMSKVVHMDRWSVEVIPQQTEEKSDPVPFQIINNYFSIGVDASIAHRFHIMREKYPEKFNSRMKNKLWYFEFATSESIFSTCKKLEESLTVEICGKPLDLSNLSLEGIAVLNIPSMHGGSNLWGDTRRPHGDIYGINQALGATAKVITDPDILKTCVPDLSDKRLEVVGLEGAIEMGQIYTKLKNAGRRLAKCSEITFHTTKTLPMQIDGEPWMQTPCTIKITHKNQMPMLMGPPPRSTNFFGFLS

Target ORF information:

RefSeq Version	NM_201445.1
Organism	Homo sapiens(human)
Definition	Homo sapiens diacylglycerol kinase alpha (DGKA), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_201445.1

ORF Insert Sequence:

ATGGCCAAGG AGAGGGGCCT AATAAGCCCC AGTGATTTTG CCCAGCTGCA AAAATACATG 
GAATACTCCA CCAAAAAGGT CAGTGATGTC CTAAAGCTCT TCGAGGATGG CGAGATGGCT 
AAATATGTCC AAGGAGATGC CATTGGGTAC GAGGGATTCC AGCAATTCCT GAAAATCTAT 
CTCGAAGTGG ATAATGTTCC CAGACACCTA AGCCTGGCAC TGTTTCAATC CTTTGAGACT 
GGTCACTGCT TAAATGAGAC AAATGTGACA AAAGATGTGG TGTGTCTCAA TGATGTTTCC 
TGCTACTTTT CCCTTCTGGA GGGTGGTCGG CCAGAAGACA AGTTAGAATT CACCTTCAAG 
CTGTACGACA CGGACAGAAA TGGGATCCTG GACAGCTCAG AAGTGGACAA AATTATCCTA 
CAGATGATGC GAGTGGCTGA ATACCTGGAT TGGGATGTGT CTGAGCTGAG GCCGATTCTT 
CAGGAGATGA TGAAAGAGAT TGACTATGAT GGCAGTGGCT CTGTCTCTCA AGCTGAGTGG 
GTCCGGGCTG GGGCCACCAC CGTGCCACTG CTAGTGCTGC TGGGTCTGGA GATGACTCTG 
AAGGACGACG GACAGCACAT GTGGAGGCCC AAGAGGTTCC CCAGACCAGT CTACTGCAAT 
CTGTGCGAGT CAAGCATTGG TCTTGGCAAA CAGGGACTGA GCTGTAACCT CTGTAAGTAC 
ACTGTTCACG ACCAGTGTGC CATGAAAGCC CTGCCTTGTG AAGTCAGCAC CTATGCCAAG 
TCTCGGAAGG ACATTGGTGT CCAATCACAT GTGTGGGTGC GAGGAGGCTG TGAGTCCGGG 
CGCTGCGACC GCTGTCAGAA AAAGATCCGG ATCTACCACA GTCTGACCGG GCTGCATTGT 
GTATGGTGCC ACCTAGAGAT CCACGATGAC TGCCTGCAAG CGGTGGGCCA TGAGTGTGAC 
TGTGGGCTGC TCCGGGATCA CATCCTGCCT CCATCTTCCA TCTATCCCAG TGTCCTGGCC 
TCTGGACCGG ATCGTAAAAA TAGCAAAACA AGCCAGAAGA CCATGGATGA TTTAAATTTG 
AGCACCTCTG AGGCTCTGCG GATTGACCCT GTTCCTAACA CCCACCCACT TCTCGTCTTT 
GTCAATCCTA AGAGTGGCGG GAAGCAGGGG CAAAGGGTGC TCTGGAAGTT CCAGTATATA 
TTAAACCCTC GACAGGTGTT CAACCTCCTA AAGGATGGTC CTGAGATAGG GCTCCGATTA 
TTCAAGGATG TTCCTGATAG CCGGATTTTG GTGTGTGGTG GAGACGGCAC AGTAGGCTGG 
ATTCTAGAGA CCATTGACAA AGCTAACTTG CCAGTTTTGC CTCCTGTTGC TGTGTTGCCC 
CTGGGTACTG GAAATGATCT GGCTCGATGC CTAAGATGGG GAGGAGGTTA TGAAGGACAG 
AATCTGGCAA AGATCCTCAA GGATTTAGAG ATGAGTAAAG TGGTACATAT GGATCGATGG 
TCTGTGGAGG TGATACCTCA ACAAACTGAA GAAAAAAGTG ACCCAGTCCC CTTTCAAATC 
ATCAATAACT ACTTCTCTAT TGGCGTGGAT GCCTCTATTG CTCATCGATT CCACATCATG 
CGAGAGAAAT ATCCGGAGAA GTTCAACAGC AGAATGAAGA ACAAGCTATG GTACTTCGAA 
TTTGCCACAT CTGAATCCAT CTTCTCAACA TGCAAAAAGC TGGAGGAGTC TTTGACAGTT 
GAGATCTGTG GGAAACCGCT GGATCTGAGC AACCTGTCCC TAGAAGGCAT CGCAGTGCTA 
AACATCCCTA GCATGCATGG TGGCTCCAAC CTCTGGGGTG ATACCAGGAG ACCCCATGGG 
GATATCTATG GGATCAACCA GGCCTTAGGT GCTACAGCTA AAGTCATCAC CGACCCTGAT 
ATCCTGAAAA CCTGTGTACC AGACCTAAGT GACAAGAGAC TGGAAGTGGT TGGGCTGGAG 
GGTGCAATTG AGATGGGCCA AATCTATACC AAGCTCAAGA ATGCTGGACG TCGGCTGGCC 
AAGTGCTCTG AGATCACCTT CCACACCACA AAAACCCTTC CCATGCAAAT TGACGGAGAA 
CCCTGGATGC AGACGCCCTG TACAATCAAG ATCACCCACA AGAACCAGAT GCCCATGCTC 
ATGGGCCCAC CCCCCCGCTC CACCAATTTC TTTGGCTTCT TGAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DGKA ( NM_201554.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_963848.1 Homo sapiens diacylglycerol kinase alpha (DGKA), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OHu02866
Clone ID Related Accession (Same CDS sequence)	NM_001351033.1 , NM_201445.1 , NM_201554.1 , NM_001345.4 , XM_011537992.1 , XM_011537991.1 , XM_011537993.1 , NM_001345.5 , NM_201444.2 , NM_001351034.1 , XM_011537993.2
Accession Version	NM_201554.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2208bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-30
Organism	Homo sapiens(human)
Product	diacylglycerol kinase alpha isoform a
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BF448612.1, BC023523.2, BC031870.2 and AF064768.1. Transcript Variant: This variant (4), as well as variants 1-3, 5, and 6, encodes isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.57202.1, SRR1803611.230752.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCAAGG AGAGGGGCCT AATAAGCCCC AGTGATTTTG CCCAGCTGCA AAAATACATG 
GAATACTCCA CCAAAAAGGT CAGTGATGTC CTAAAGCTCT TCGAGGATGG CGAGATGGCT 
AAATATGTCC AAGGAGATGC CATTGGGTAC GAGGGATTCC AGCAATTCCT GAAAATCTAT 
CTCGAAGTGG ATAATGTTCC CAGACACCTA AGCCTGGCAC TGTTTCAATC CTTTGAGACT 
GGTCACTGCT TAAATGAGAC AAATGTGACA AAAGATGTGG TGTGTCTCAA TGATGTTTCC 
TGCTACTTTT CCCTTCTGGA GGGTGGTCGG CCAGAAGACA AGTTAGAATT CACCTTCAAG 
CTGTACGACA CGGACAGAAA TGGGATCCTG GACAGCTCAG AAGTGGACAA AATTATCCTA 
CAGATGATGC GAGTGGCTGA ATACCTGGAT TGGGATGTGT CTGAGCTGAG GCCGATTCTT 
CAGGAGATGA TGAAAGAGAT TGACTATGAT GGCAGTGGCT CTGTCTCTCA AGCTGAGTGG 
GTCCGGGCTG GGGCCACCAC CGTGCCACTG CTAGTGCTGC TGGGTCTGGA GATGACTCTG 
AAGGACGACG GACAGCACAT GTGGAGGCCC AAGAGGTTCC CCAGACCAGT CTACTGCAAT 
CTGTGCGAGT CAAGCATTGG TCTTGGCAAA CAGGGACTGA GCTGTAACCT CTGTAAGTAC 
ACTGTTCACG ACCAGTGTGC CATGAAAGCC CTGCCTTGTG AAGTCAGCAC CTATGCCAAG 
TCTCGGAAGG ACATTGGTGT CCAATCACAT GTGTGGGTGC GAGGAGGCTG TGAGTCCGGG 
CGCTGCGACC GCTGTCAGAA AAAGATCCGG ATCTACCACA GTCTGACCGG GCTGCATTGT 
GTATGGTGCC ACCTAGAGAT CCACGATGAC TGCCTGCAAG CGGTGGGCCA TGAGTGTGAC 
TGTGGGCTGC TCCGGGATCA CATCCTGCCT CCATCTTCCA TCTATCCCAG TGTCCTGGCC 
TCTGGACCGG ATCGTAAAAA TAGCAAAACA AGCCAGAAGA CCATGGATGA TTTAAATTTG 
AGCACCTCTG AGGCTCTGCG GATTGACCCT GTTCCTAACA CCCACCCACT TCTCGTCTTT 
GTCAATCCTA AGAGTGGCGG GAAGCAGGGG CAAAGGGTGC TCTGGAAGTT CCAGTATATA 
TTAAACCCTC GACAGGTGTT CAACCTCCTA AAGGATGGTC CTGAGATAGG GCTCCGATTA 
TTCAAGGATG TTCCTGATAG CCGGATTTTG GTGTGTGGTG GAGACGGCAC AGTAGGCTGG 
ATTCTAGAGA CCATTGACAA AGCTAACTTG CCAGTTTTGC CTCCTGTTGC TGTGTTGCCC 
CTGGGTACTG GAAATGATCT GGCTCGATGC CTAAGATGGG GAGGAGGTTA TGAAGGACAG 
AATCTGGCAA AGATCCTCAA GGATTTAGAG ATGAGTAAAG TGGTACATAT GGATCGATGG 
TCTGTGGAGG TGATACCTCA ACAAACTGAA GAAAAAAGTG ACCCAGTCCC CTTTCAAATC 
ATCAATAACT ACTTCTCTAT TGGCGTGGAT GCCTCTATTG CTCATCGATT CCACATCATG 
CGAGAGAAAT ATCCGGAGAA GTTCAACAGC AGAATGAAGA ACAAGCTATG GTACTTCGAA 
TTTGCCACAT CTGAATCCAT CTTCTCAACA TGCAAAAAGC TGGAGGAGTC TTTGACAGTT 
GAGATCTGTG GGAAACCGCT GGATCTGAGC AACCTGTCCC TAGAAGGCAT CGCAGTGCTA 
AACATCCCTA GCATGCATGG TGGCTCCAAC CTCTGGGGTG ATACCAGGAG ACCCCATGGG 
GATATCTATG GGATCAACCA GGCCTTAGGT GCTACAGCTA AAGTCATCAC CGACCCTGAT 
ATCCTGAAAA CCTGTGTACC AGACCTAAGT GACAAGAGAC TGGAAGTGGT TGGGCTGGAG 
GGTGCAATTG AGATGGGCCA AATCTATACC AAGCTCAAGA ATGCTGGACG TCGGCTGGCC 
AAGTGCTCTG AGATCACCTT CCACACCACA AAAACCCTTC CCATGCAAAT TGACGGAGAA 
CCCTGGATGC AGACGCCCTG TACAATCAAG ATCACCCACA AGAACCAGAT GCCCATGCTC 
ATGGGCCCAC CCCCCCGCTC CACCAATTTC TTTGGCTTCT TGAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_963848.1
CDS	178..2385
Translation	MAKERGLISPSDFAQLQKYMEYSTKKVSDVLKLFEDGEMAKYVQGDAIGYEGFQQFLKIYLEVDNVPRHLSLALFQSFETGHCLNETNVTKDVVCLNDVSCYFSLLEGGRPEDKLEFTFKLYDTDRNGILDSSEVDKIILQMMRVAEYLDWDVSELRPILQEMMKEIDYDGSGSVSQAEWVRAGATTVPLLVLLGLEMTLKDDGQHMWRPKRFPRPVYCNLCESSIGLGKQGLSCNLCKYTVHDQCAMKALPCEVSTYAKSRKDIGVQSHVWVRGGCESGRCDRCQKKIRIYHSLTGLHCVWCHLEIHDDCLQAVGHECDCGLLRDHILPPSSIYPSVLASGPDRKNSKTSQKTMDDLNLSTSEALRIDPVPNTHPLLVFVNPKSGGKQGQRVLWKFQYILNPRQVFNLLKDGPEIGLRLFKDVPDSRILVCGGDGTVGWILETIDKANLPVLPPVAVLPLGTGNDLARCLRWGGGYEGQNLAKILKDLEMSKVVHMDRWSVEVIPQQTEEKSDPVPFQIINNYFSIGVDASIAHRFHIMREKYPEKFNSRMKNKLWYFEFATSESIFSTCKKLEESLTVEICGKPLDLSNLSLEGIAVLNIPSMHGGSNLWGDTRRPHGDIYGINQALGATAKVITDPDILKTCVPDLSDKRLEVVGLEGAIEMGQIYTKLKNAGRRLAKCSEITFHTTKTLPMQIDGEPWMQTPCTIKITHKNQMPMLMGPPPRSTNFFGFLS

Target ORF information:

RefSeq Version	NM_201554.1
Organism	Homo sapiens(human)
Definition	Homo sapiens diacylglycerol kinase alpha (DGKA), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_201554.1

ORF Insert Sequence:

ATGGCCAAGG AGAGGGGCCT AATAAGCCCC AGTGATTTTG CCCAGCTGCA AAAATACATG 
GAATACTCCA CCAAAAAGGT CAGTGATGTC CTAAAGCTCT TCGAGGATGG CGAGATGGCT 
AAATATGTCC AAGGAGATGC CATTGGGTAC GAGGGATTCC AGCAATTCCT GAAAATCTAT 
CTCGAAGTGG ATAATGTTCC CAGACACCTA AGCCTGGCAC TGTTTCAATC CTTTGAGACT 
GGTCACTGCT TAAATGAGAC AAATGTGACA AAAGATGTGG TGTGTCTCAA TGATGTTTCC 
TGCTACTTTT CCCTTCTGGA GGGTGGTCGG CCAGAAGACA AGTTAGAATT CACCTTCAAG 
CTGTACGACA CGGACAGAAA TGGGATCCTG GACAGCTCAG AAGTGGACAA AATTATCCTA 
CAGATGATGC GAGTGGCTGA ATACCTGGAT TGGGATGTGT CTGAGCTGAG GCCGATTCTT 
CAGGAGATGA TGAAAGAGAT TGACTATGAT GGCAGTGGCT CTGTCTCTCA AGCTGAGTGG 
GTCCGGGCTG GGGCCACCAC CGTGCCACTG CTAGTGCTGC TGGGTCTGGA GATGACTCTG 
AAGGACGACG GACAGCACAT GTGGAGGCCC AAGAGGTTCC CCAGACCAGT CTACTGCAAT 
CTGTGCGAGT CAAGCATTGG TCTTGGCAAA CAGGGACTGA GCTGTAACCT CTGTAAGTAC 
ACTGTTCACG ACCAGTGTGC CATGAAAGCC CTGCCTTGTG AAGTCAGCAC CTATGCCAAG 
TCTCGGAAGG ACATTGGTGT CCAATCACAT GTGTGGGTGC GAGGAGGCTG TGAGTCCGGG 
CGCTGCGACC GCTGTCAGAA AAAGATCCGG ATCTACCACA GTCTGACCGG GCTGCATTGT 
GTATGGTGCC ACCTAGAGAT CCACGATGAC TGCCTGCAAG CGGTGGGCCA TGAGTGTGAC 
TGTGGGCTGC TCCGGGATCA CATCCTGCCT CCATCTTCCA TCTATCCCAG TGTCCTGGCC 
TCTGGACCGG ATCGTAAAAA TAGCAAAACA AGCCAGAAGA CCATGGATGA TTTAAATTTG 
AGCACCTCTG AGGCTCTGCG GATTGACCCT GTTCCTAACA CCCACCCACT TCTCGTCTTT 
GTCAATCCTA AGAGTGGCGG GAAGCAGGGG CAAAGGGTGC TCTGGAAGTT CCAGTATATA 
TTAAACCCTC GACAGGTGTT CAACCTCCTA AAGGATGGTC CTGAGATAGG GCTCCGATTA 
TTCAAGGATG TTCCTGATAG CCGGATTTTG GTGTGTGGTG GAGACGGCAC AGTAGGCTGG 
ATTCTAGAGA CCATTGACAA AGCTAACTTG CCAGTTTTGC CTCCTGTTGC TGTGTTGCCC 
CTGGGTACTG GAAATGATCT GGCTCGATGC CTAAGATGGG GAGGAGGTTA TGAAGGACAG 
AATCTGGCAA AGATCCTCAA GGATTTAGAG ATGAGTAAAG TGGTACATAT GGATCGATGG 
TCTGTGGAGG TGATACCTCA ACAAACTGAA GAAAAAAGTG ACCCAGTCCC CTTTCAAATC 
ATCAATAACT ACTTCTCTAT TGGCGTGGAT GCCTCTATTG CTCATCGATT CCACATCATG 
CGAGAGAAAT ATCCGGAGAA GTTCAACAGC AGAATGAAGA ACAAGCTATG GTACTTCGAA 
TTTGCCACAT CTGAATCCAT CTTCTCAACA TGCAAAAAGC TGGAGGAGTC TTTGACAGTT 
GAGATCTGTG GGAAACCGCT GGATCTGAGC AACCTGTCCC TAGAAGGCAT CGCAGTGCTA 
AACATCCCTA GCATGCATGG TGGCTCCAAC CTCTGGGGTG ATACCAGGAG ACCCCATGGG 
GATATCTATG GGATCAACCA GGCCTTAGGT GCTACAGCTA AAGTCATCAC CGACCCTGAT 
ATCCTGAAAA CCTGTGTACC AGACCTAAGT GACAAGAGAC TGGAAGTGGT TGGGCTGGAG 
GGTGCAATTG AGATGGGCCA AATCTATACC AAGCTCAAGA ATGCTGGACG TCGGCTGGCC 
AAGTGCTCTG AGATCACCTT CCACACCACA AAAACCCTTC CCATGCAAAT TGACGGAGAA 
CCCTGGATGC AGACGCCCTG TACAATCAAG ATCACCCACA AGAACCAGAT GCCCATGCTC 
ATGGGCCCAC CCCCCCGCTC CACCAATTTC TTTGGCTTCT TGAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DGKA ( NM_001345.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_001336.2 Homo sapiens diacylglycerol kinase alpha (DGKA), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu02866
Clone ID Related Accession (Same CDS sequence)	NM_001351033.1 , NM_201445.1 , NM_201554.1 , NM_001345.4 , XM_011537992.1 , XM_011537991.1 , XM_011537993.1 , NM_001345.5 , NM_201444.2 , NM_001351034.1 , XM_011537993.2
Accession Version	NM_001345.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2208bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-11-19
Organism	Homo sapiens(human)
Product	diacylglycerol kinase alpha isoform a
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BM836118.1, BQ943057.1, BC031870.2, BC023523.2 and AF064768.1. On Jan 30, 2004 this sequence version replaced NM_001345.3. Transcript Variant: This variant (3), as well as variants 1, 2, and 4-6, encodes isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.209972.1, SRR1803612.235168.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCAAGG AGAGGGGCCT AATAAGCCCC AGTGATTTTG CCCAGCTGCA AAAATACATG 
GAATACTCCA CCAAAAAGGT CAGTGATGTC CTAAAGCTCT TCGAGGATGG CGAGATGGCT 
AAATATGTCC AAGGAGATGC CATTGGGTAC GAGGGATTCC AGCAATTCCT GAAAATCTAT 
CTCGAAGTGG ATAATGTTCC CAGACACCTA AGCCTGGCAC TGTTTCAATC CTTTGAGACT 
GGTCACTGCT TAAATGAGAC AAATGTGACA AAAGATGTGG TGTGTCTCAA TGATGTTTCC 
TGCTACTTTT CCCTTCTGGA GGGTGGTCGG CCAGAAGACA AGTTAGAATT CACCTTCAAG 
CTGTACGACA CGGACAGAAA TGGGATCCTG GACAGCTCAG AAGTGGACAA AATTATCCTA 
CAGATGATGC GAGTGGCTGA ATACCTGGAT TGGGATGTGT CTGAGCTGAG GCCGATTCTT 
CAGGAGATGA TGAAAGAGAT TGACTATGAT GGCAGTGGCT CTGTCTCTCA AGCTGAGTGG 
GTCCGGGCTG GGGCCACCAC CGTGCCACTG CTAGTGCTGC TGGGTCTGGA GATGACTCTG 
AAGGACGACG GACAGCACAT GTGGAGGCCC AAGAGGTTCC CCAGACCAGT CTACTGCAAT 
CTGTGCGAGT CAAGCATTGG TCTTGGCAAA CAGGGACTGA GCTGTAACCT CTGTAAGTAC 
ACTGTTCACG ACCAGTGTGC CATGAAAGCC CTGCCTTGTG AAGTCAGCAC CTATGCCAAG 
TCTCGGAAGG ACATTGGTGT CCAATCACAT GTGTGGGTGC GAGGAGGCTG TGAGTCCGGG 
CGCTGCGACC GCTGTCAGAA AAAGATCCGG ATCTACCACA GTCTGACCGG GCTGCATTGT 
GTATGGTGCC ACCTAGAGAT CCACGATGAC TGCCTGCAAG CGGTGGGCCA TGAGTGTGAC 
TGTGGGCTGC TCCGGGATCA CATCCTGCCT CCATCTTCCA TCTATCCCAG TGTCCTGGCC 
TCTGGACCGG ATCGTAAAAA TAGCAAAACA AGCCAGAAGA CCATGGATGA TTTAAATTTG 
AGCACCTCTG AGGCTCTGCG GATTGACCCT GTTCCTAACA CCCACCCACT TCTCGTCTTT 
GTCAATCCTA AGAGTGGCGG GAAGCAGGGG CAAAGGGTGC TCTGGAAGTT CCAGTATATA 
TTAAACCCTC GACAGGTGTT CAACCTCCTA AAGGATGGTC CTGAGATAGG GCTCCGATTA 
TTCAAGGATG TTCCTGATAG CCGGATTTTG GTGTGTGGTG GAGACGGCAC AGTAGGCTGG 
ATTCTAGAGA CCATTGACAA AGCTAACTTG CCAGTTTTGC CTCCTGTTGC TGTGTTGCCC 
CTGGGTACTG GAAATGATCT GGCTCGATGC CTAAGATGGG GAGGAGGTTA TGAAGGACAG 
AATCTGGCAA AGATCCTCAA GGATTTAGAG ATGAGTAAAG TGGTACATAT GGATCGATGG 
TCTGTGGAGG TGATACCTCA ACAAACTGAA GAAAAAAGTG ACCCAGTCCC CTTTCAAATC 
ATCAATAACT ACTTCTCTAT TGGCGTGGAT GCCTCTATTG CTCATCGATT CCACATCATG 
CGAGAGAAAT ATCCGGAGAA GTTCAACAGC AGAATGAAGA ACAAGCTATG GTACTTCGAA 
TTTGCCACAT CTGAATCCAT CTTCTCAACA TGCAAAAAGC TGGAGGAGTC TTTGACAGTT 
GAGATCTGTG GGAAACCGCT GGATCTGAGC AACCTGTCCC TAGAAGGCAT CGCAGTGCTA 
AACATCCCTA GCATGCATGG TGGCTCCAAC CTCTGGGGTG ATACCAGGAG ACCCCATGGG 
GATATCTATG GGATCAACCA GGCCTTAGGT GCTACAGCTA AAGTCATCAC CGACCCTGAT 
ATCCTGAAAA CCTGTGTACC AGACCTAAGT GACAAGAGAC TGGAAGTGGT TGGGCTGGAG 
GGTGCAATTG AGATGGGCCA AATCTATACC AAGCTCAAGA ATGCTGGACG TCGGCTGGCC 
AAGTGCTCTG AGATCACCTT CCACACCACA AAAACCCTTC CCATGCAAAT TGACGGAGAA 
CCCTGGATGC AGACGCCCTG TACAATCAAG ATCACCCACA AGAACCAGAT GCCCATGCTC 
ATGGGCCCAC CCCCCCGCTC CACCAATTTC TTTGGCTTCT TGAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001336.2
CDS	265..2472
Translation	MAKERGLISPSDFAQLQKYMEYSTKKVSDVLKLFEDGEMAKYVQGDAIGYEGFQQFLKIYLEVDNVPRHLSLALFQSFETGHCLNETNVTKDVVCLNDVSCYFSLLEGGRPEDKLEFTFKLYDTDRNGILDSSEVDKIILQMMRVAEYLDWDVSELRPILQEMMKEIDYDGSGSVSQAEWVRAGATTVPLLVLLGLEMTLKDDGQHMWRPKRFPRPVYCNLCESSIGLGKQGLSCNLCKYTVHDQCAMKALPCEVSTYAKSRKDIGVQSHVWVRGGCESGRCDRCQKKIRIYHSLTGLHCVWCHLEIHDDCLQAVGHECDCGLLRDHILPPSSIYPSVLASGPDRKNSKTSQKTMDDLNLSTSEALRIDPVPNTHPLLVFVNPKSGGKQGQRVLWKFQYILNPRQVFNLLKDGPEIGLRLFKDVPDSRILVCGGDGTVGWILETIDKANLPVLPPVAVLPLGTGNDLARCLRWGGGYEGQNLAKILKDLEMSKVVHMDRWSVEVIPQQTEEKSDPVPFQIINNYFSIGVDASIAHRFHIMREKYPEKFNSRMKNKLWYFEFATSESIFSTCKKLEESLTVEICGKPLDLSNLSLEGIAVLNIPSMHGGSNLWGDTRRPHGDIYGINQALGATAKVITDPDILKTCVPDLSDKRLEVVGLEGAIEMGQIYTKLKNAGRRLAKCSEITFHTTKTLPMQIDGEPWMQTPCTIKITHKNQMPMLMGPPPRSTNFFGFLS

Target ORF information:

RefSeq Version	NM_001345.4
Organism	Homo sapiens(human)
Definition	Homo sapiens diacylglycerol kinase alpha (DGKA), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001345.4

ORF Insert Sequence:

ATGGCCAAGG AGAGGGGCCT AATAAGCCCC AGTGATTTTG CCCAGCTGCA AAAATACATG 
GAATACTCCA CCAAAAAGGT CAGTGATGTC CTAAAGCTCT TCGAGGATGG CGAGATGGCT 
AAATATGTCC AAGGAGATGC CATTGGGTAC GAGGGATTCC AGCAATTCCT GAAAATCTAT 
CTCGAAGTGG ATAATGTTCC CAGACACCTA AGCCTGGCAC TGTTTCAATC CTTTGAGACT 
GGTCACTGCT TAAATGAGAC AAATGTGACA AAAGATGTGG TGTGTCTCAA TGATGTTTCC 
TGCTACTTTT CCCTTCTGGA GGGTGGTCGG CCAGAAGACA AGTTAGAATT CACCTTCAAG 
CTGTACGACA CGGACAGAAA TGGGATCCTG GACAGCTCAG AAGTGGACAA AATTATCCTA 
CAGATGATGC GAGTGGCTGA ATACCTGGAT TGGGATGTGT CTGAGCTGAG GCCGATTCTT 
CAGGAGATGA TGAAAGAGAT TGACTATGAT GGCAGTGGCT CTGTCTCTCA AGCTGAGTGG 
GTCCGGGCTG GGGCCACCAC CGTGCCACTG CTAGTGCTGC TGGGTCTGGA GATGACTCTG 
AAGGACGACG GACAGCACAT GTGGAGGCCC AAGAGGTTCC CCAGACCAGT CTACTGCAAT 
CTGTGCGAGT CAAGCATTGG TCTTGGCAAA CAGGGACTGA GCTGTAACCT CTGTAAGTAC 
ACTGTTCACG ACCAGTGTGC CATGAAAGCC CTGCCTTGTG AAGTCAGCAC CTATGCCAAG 
TCTCGGAAGG ACATTGGTGT CCAATCACAT GTGTGGGTGC GAGGAGGCTG TGAGTCCGGG 
CGCTGCGACC GCTGTCAGAA AAAGATCCGG ATCTACCACA GTCTGACCGG GCTGCATTGT 
GTATGGTGCC ACCTAGAGAT CCACGATGAC TGCCTGCAAG CGGTGGGCCA TGAGTGTGAC 
TGTGGGCTGC TCCGGGATCA CATCCTGCCT CCATCTTCCA TCTATCCCAG TGTCCTGGCC 
TCTGGACCGG ATCGTAAAAA TAGCAAAACA AGCCAGAAGA CCATGGATGA TTTAAATTTG 
AGCACCTCTG AGGCTCTGCG GATTGACCCT GTTCCTAACA CCCACCCACT TCTCGTCTTT 
GTCAATCCTA AGAGTGGCGG GAAGCAGGGG CAAAGGGTGC TCTGGAAGTT CCAGTATATA 
TTAAACCCTC GACAGGTGTT CAACCTCCTA AAGGATGGTC CTGAGATAGG GCTCCGATTA 
TTCAAGGATG TTCCTGATAG CCGGATTTTG GTGTGTGGTG GAGACGGCAC AGTAGGCTGG 
ATTCTAGAGA CCATTGACAA AGCTAACTTG CCAGTTTTGC CTCCTGTTGC TGTGTTGCCC 
CTGGGTACTG GAAATGATCT GGCTCGATGC CTAAGATGGG GAGGAGGTTA TGAAGGACAG 
AATCTGGCAA AGATCCTCAA GGATTTAGAG ATGAGTAAAG TGGTACATAT GGATCGATGG 
TCTGTGGAGG TGATACCTCA ACAAACTGAA GAAAAAAGTG ACCCAGTCCC CTTTCAAATC 
ATCAATAACT ACTTCTCTAT TGGCGTGGAT GCCTCTATTG CTCATCGATT CCACATCATG 
CGAGAGAAAT ATCCGGAGAA GTTCAACAGC AGAATGAAGA ACAAGCTATG GTACTTCGAA 
TTTGCCACAT CTGAATCCAT CTTCTCAACA TGCAAAAAGC TGGAGGAGTC TTTGACAGTT 
GAGATCTGTG GGAAACCGCT GGATCTGAGC AACCTGTCCC TAGAAGGCAT CGCAGTGCTA 
AACATCCCTA GCATGCATGG TGGCTCCAAC CTCTGGGGTG ATACCAGGAG ACCCCATGGG 
GATATCTATG GGATCAACCA GGCCTTAGGT GCTACAGCTA AAGTCATCAC CGACCCTGAT 
ATCCTGAAAA CCTGTGTACC AGACCTAAGT GACAAGAGAC TGGAAGTGGT TGGGCTGGAG 
GGTGCAATTG AGATGGGCCA AATCTATACC AAGCTCAAGA ATGCTGGACG TCGGCTGGCC 
AAGTGCTCTG AGATCACCTT CCACACCACA AAAACCCTTC CCATGCAAAT TGACGGAGAA 
CCCTGGATGC AGACGCCCTG TACAATCAAG ATCACCCACA AGAACCAGAT GCCCATGCTC 
ATGGGCCCAC CCCCCCGCTC CACCAATTTC TTTGGCTTCT TGAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DGKA ( NM_001345.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_001336.2 Homo sapiens diacylglycerol kinase alpha (DGKA), transcript variant 3, mRNA.

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CloneID	OHu02866
Clone ID Related Accession (Same CDS sequence)	NM_001351033.1 , NM_201445.1 , NM_201554.1 , NM_001345.4 , XM_011537992.1 , XM_011537991.1 , XM_011537993.1 , NM_001345.5 , NM_201444.2 , NM_001351034.1 , XM_011537993.2
Accession Version	NM_001345.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2208bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-30
Organism	Homo sapiens(human)
Product	diacylglycerol kinase alpha isoform a
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BM836118.1, BQ943057.1, BC031870.2 and BC023523.2. On Nov 22, 2018 this sequence version replaced NM_001345.4. Transcript Variant: This variant (3), as well as variants 1, 2, and 4-6, encodes isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.209972.1, SRR1803612.235168.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000331886.10/ ENSP00000328405.5 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCCAAGG AGAGGGGCCT AATAAGCCCC AGTGATTTTG CCCAGCTGCA AAAATACATG 
GAATACTCCA CCAAAAAGGT CAGTGATGTC CTAAAGCTCT TCGAGGATGG CGAGATGGCT 
AAATATGTCC AAGGAGATGC CATTGGGTAC GAGGGATTCC AGCAATTCCT GAAAATCTAT 
CTCGAAGTGG ATAATGTTCC CAGACACCTA AGCCTGGCAC TGTTTCAATC CTTTGAGACT 
GGTCACTGCT TAAATGAGAC AAATGTGACA AAAGATGTGG TGTGTCTCAA TGATGTTTCC 
TGCTACTTTT CCCTTCTGGA GGGTGGTCGG CCAGAAGACA AGTTAGAATT CACCTTCAAG 
CTGTACGACA CGGACAGAAA TGGGATCCTG GACAGCTCAG AAGTGGACAA AATTATCCTA 
CAGATGATGC GAGTGGCTGA ATACCTGGAT TGGGATGTGT CTGAGCTGAG GCCGATTCTT 
CAGGAGATGA TGAAAGAGAT TGACTATGAT GGCAGTGGCT CTGTCTCTCA AGCTGAGTGG 
GTCCGGGCTG GGGCCACCAC CGTGCCACTG CTAGTGCTGC TGGGTCTGGA GATGACTCTG 
AAGGACGACG GACAGCACAT GTGGAGGCCC AAGAGGTTCC CCAGACCAGT CTACTGCAAT 
CTGTGCGAGT CAAGCATTGG TCTTGGCAAA CAGGGACTGA GCTGTAACCT CTGTAAGTAC 
ACTGTTCACG ACCAGTGTGC CATGAAAGCC CTGCCTTGTG AAGTCAGCAC CTATGCCAAG 
TCTCGGAAGG ACATTGGTGT CCAATCACAT GTGTGGGTGC GAGGAGGCTG TGAGTCCGGG 
CGCTGCGACC GCTGTCAGAA AAAGATCCGG ATCTACCACA GTCTGACCGG GCTGCATTGT 
GTATGGTGCC ACCTAGAGAT CCACGATGAC TGCCTGCAAG CGGTGGGCCA TGAGTGTGAC 
TGTGGGCTGC TCCGGGATCA CATCCTGCCT CCATCTTCCA TCTATCCCAG TGTCCTGGCC 
TCTGGACCGG ATCGTAAAAA TAGCAAAACA AGCCAGAAGA CCATGGATGA TTTAAATTTG 
AGCACCTCTG AGGCTCTGCG GATTGACCCT GTTCCTAACA CCCACCCACT TCTCGTCTTT 
GTCAATCCTA AGAGTGGCGG GAAGCAGGGG CAAAGGGTGC TCTGGAAGTT CCAGTATATA 
TTAAACCCTC GACAGGTGTT CAACCTCCTA AAGGATGGTC CTGAGATAGG GCTCCGATTA 
TTCAAGGATG TTCCTGATAG CCGGATTTTG GTGTGTGGTG GAGACGGCAC AGTAGGCTGG 
ATTCTAGAGA CCATTGACAA AGCTAACTTG CCAGTTTTGC CTCCTGTTGC TGTGTTGCCC 
CTGGGTACTG GAAATGATCT GGCTCGATGC CTAAGATGGG GAGGAGGTTA TGAAGGACAG 
AATCTGGCAA AGATCCTCAA GGATTTAGAG ATGAGTAAAG TGGTACATAT GGATCGATGG 
TCTGTGGAGG TGATACCTCA ACAAACTGAA GAAAAAAGTG ACCCAGTCCC CTTTCAAATC 
ATCAATAACT ACTTCTCTAT TGGCGTGGAT GCCTCTATTG CTCATCGATT CCACATCATG 
CGAGAGAAAT ATCCGGAGAA GTTCAACAGC AGAATGAAGA ACAAGCTATG GTACTTCGAA 
TTTGCCACAT CTGAATCCAT CTTCTCAACA TGCAAAAAGC TGGAGGAGTC TTTGACAGTT 
GAGATCTGTG GGAAACCGCT GGATCTGAGC AACCTGTCCC TAGAAGGCAT CGCAGTGCTA 
AACATCCCTA GCATGCATGG TGGCTCCAAC CTCTGGGGTG ATACCAGGAG ACCCCATGGG 
GATATCTATG GGATCAACCA GGCCTTAGGT GCTACAGCTA AAGTCATCAC CGACCCTGAT 
ATCCTGAAAA CCTGTGTACC AGACCTAAGT GACAAGAGAC TGGAAGTGGT TGGGCTGGAG 
GGTGCAATTG AGATGGGCCA AATCTATACC AAGCTCAAGA ATGCTGGACG TCGGCTGGCC 
AAGTGCTCTG AGATCACCTT CCACACCACA AAAACCCTTC CCATGCAAAT TGACGGAGAA 
CCCTGGATGC AGACGCCCTG TACAATCAAG ATCACCCACA AGAACCAGAT GCCCATGCTC 
ATGGGCCCAC CCCCCCGCTC CACCAATTTC TTTGGCTTCT TGAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001336.2
CDS	265..2472
Translation	MAKERGLISPSDFAQLQKYMEYSTKKVSDVLKLFEDGEMAKYVQGDAIGYEGFQQFLKIYLEVDNVPRHLSLALFQSFETGHCLNETNVTKDVVCLNDVSCYFSLLEGGRPEDKLEFTFKLYDTDRNGILDSSEVDKIILQMMRVAEYLDWDVSELRPILQEMMKEIDYDGSGSVSQAEWVRAGATTVPLLVLLGLEMTLKDDGQHMWRPKRFPRPVYCNLCESSIGLGKQGLSCNLCKYTVHDQCAMKALPCEVSTYAKSRKDIGVQSHVWVRGGCESGRCDRCQKKIRIYHSLTGLHCVWCHLEIHDDCLQAVGHECDCGLLRDHILPPSSIYPSVLASGPDRKNSKTSQKTMDDLNLSTSEALRIDPVPNTHPLLVFVNPKSGGKQGQRVLWKFQYILNPRQVFNLLKDGPEIGLRLFKDVPDSRILVCGGDGTVGWILETIDKANLPVLPPVAVLPLGTGNDLARCLRWGGGYEGQNLAKILKDLEMSKVVHMDRWSVEVIPQQTEEKSDPVPFQIINNYFSIGVDASIAHRFHIMREKYPEKFNSRMKNKLWYFEFATSESIFSTCKKLEESLTVEICGKPLDLSNLSLEGIAVLNIPSMHGGSNLWGDTRRPHGDIYGINQALGATAKVITDPDILKTCVPDLSDKRLEVVGLEGAIEMGQIYTKLKNAGRRLAKCSEITFHTTKTLPMQIDGEPWMQTPCTIKITHKNQMPMLMGPPPRSTNFFGFLS

Target ORF information:

RefSeq Version	NM_001345.5
Organism	Homo sapiens(human)
Definition	Homo sapiens diacylglycerol kinase alpha (DGKA), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001345.5

ORF Insert Sequence:

ATGGCCAAGG AGAGGGGCCT AATAAGCCCC AGTGATTTTG CCCAGCTGCA AAAATACATG 
GAATACTCCA CCAAAAAGGT CAGTGATGTC CTAAAGCTCT TCGAGGATGG CGAGATGGCT 
AAATATGTCC AAGGAGATGC CATTGGGTAC GAGGGATTCC AGCAATTCCT GAAAATCTAT 
CTCGAAGTGG ATAATGTTCC CAGACACCTA AGCCTGGCAC TGTTTCAATC CTTTGAGACT 
GGTCACTGCT TAAATGAGAC AAATGTGACA AAAGATGTGG TGTGTCTCAA TGATGTTTCC 
TGCTACTTTT CCCTTCTGGA GGGTGGTCGG CCAGAAGACA AGTTAGAATT CACCTTCAAG 
CTGTACGACA CGGACAGAAA TGGGATCCTG GACAGCTCAG AAGTGGACAA AATTATCCTA 
CAGATGATGC GAGTGGCTGA ATACCTGGAT TGGGATGTGT CTGAGCTGAG GCCGATTCTT 
CAGGAGATGA TGAAAGAGAT TGACTATGAT GGCAGTGGCT CTGTCTCTCA AGCTGAGTGG 
GTCCGGGCTG GGGCCACCAC CGTGCCACTG CTAGTGCTGC TGGGTCTGGA GATGACTCTG 
AAGGACGACG GACAGCACAT GTGGAGGCCC AAGAGGTTCC CCAGACCAGT CTACTGCAAT 
CTGTGCGAGT CAAGCATTGG TCTTGGCAAA CAGGGACTGA GCTGTAACCT CTGTAAGTAC 
ACTGTTCACG ACCAGTGTGC CATGAAAGCC CTGCCTTGTG AAGTCAGCAC CTATGCCAAG 
TCTCGGAAGG ACATTGGTGT CCAATCACAT GTGTGGGTGC GAGGAGGCTG TGAGTCCGGG 
CGCTGCGACC GCTGTCAGAA AAAGATCCGG ATCTACCACA GTCTGACCGG GCTGCATTGT 
GTATGGTGCC ACCTAGAGAT CCACGATGAC TGCCTGCAAG CGGTGGGCCA TGAGTGTGAC 
TGTGGGCTGC TCCGGGATCA CATCCTGCCT CCATCTTCCA TCTATCCCAG TGTCCTGGCC 
TCTGGACCGG ATCGTAAAAA TAGCAAAACA AGCCAGAAGA CCATGGATGA TTTAAATTTG 
AGCACCTCTG AGGCTCTGCG GATTGACCCT GTTCCTAACA CCCACCCACT TCTCGTCTTT 
GTCAATCCTA AGAGTGGCGG GAAGCAGGGG CAAAGGGTGC TCTGGAAGTT CCAGTATATA 
TTAAACCCTC GACAGGTGTT CAACCTCCTA AAGGATGGTC CTGAGATAGG GCTCCGATTA 
TTCAAGGATG TTCCTGATAG CCGGATTTTG GTGTGTGGTG GAGACGGCAC AGTAGGCTGG 
ATTCTAGAGA CCATTGACAA AGCTAACTTG CCAGTTTTGC CTCCTGTTGC TGTGTTGCCC 
CTGGGTACTG GAAATGATCT GGCTCGATGC CTAAGATGGG GAGGAGGTTA TGAAGGACAG 
AATCTGGCAA AGATCCTCAA GGATTTAGAG ATGAGTAAAG TGGTACATAT GGATCGATGG 
TCTGTGGAGG TGATACCTCA ACAAACTGAA GAAAAAAGTG ACCCAGTCCC CTTTCAAATC 
ATCAATAACT ACTTCTCTAT TGGCGTGGAT GCCTCTATTG CTCATCGATT CCACATCATG 
CGAGAGAAAT ATCCGGAGAA GTTCAACAGC AGAATGAAGA ACAAGCTATG GTACTTCGAA 
TTTGCCACAT CTGAATCCAT CTTCTCAACA TGCAAAAAGC TGGAGGAGTC TTTGACAGTT 
GAGATCTGTG GGAAACCGCT GGATCTGAGC AACCTGTCCC TAGAAGGCAT CGCAGTGCTA 
AACATCCCTA GCATGCATGG TGGCTCCAAC CTCTGGGGTG ATACCAGGAG ACCCCATGGG 
GATATCTATG GGATCAACCA GGCCTTAGGT GCTACAGCTA AAGTCATCAC CGACCCTGAT 
ATCCTGAAAA CCTGTGTACC AGACCTAAGT GACAAGAGAC TGGAAGTGGT TGGGCTGGAG 
GGTGCAATTG AGATGGGCCA AATCTATACC AAGCTCAAGA ATGCTGGACG TCGGCTGGCC 
AAGTGCTCTG AGATCACCTT CCACACCACA AAAACCCTTC CCATGCAAAT TGACGGAGAA 
CCCTGGATGC AGACGCCCTG TACAATCAAG ATCACCCACA AGAACCAGAT GCCCATGCTC 
ATGGGCCCAC CCCCCCGCTC CACCAATTTC TTTGGCTTCT TGAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DGKA ( NM_201444.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_958852.1 Homo sapiens diacylglycerol kinase alpha (DGKA), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu02866
Clone ID Related Accession (Same CDS sequence)	NM_001351033.1 , NM_201445.1 , NM_201554.1 , NM_001345.4 , XM_011537992.1 , XM_011537991.1 , XM_011537993.1 , NM_001345.5 , NM_201444.2 , NM_001351034.1 , XM_011537993.2
Accession Version	NM_201444.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2208bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-30
Organism	Homo sapiens(human)
Product	diacylglycerol kinase alpha isoform a
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AI815678.1, BM910598.1, BC031870.2, BC023523.2 and AF064768.1. On Feb 3, 2004 this sequence version replaced NM_201444.1. Transcript Variant: This variant (1), as well as variants 2-6, encodes isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.189221.1, SRR1803612.26114.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCAAGG AGAGGGGCCT AATAAGCCCC AGTGATTTTG CCCAGCTGCA AAAATACATG 
GAATACTCCA CCAAAAAGGT CAGTGATGTC CTAAAGCTCT TCGAGGATGG CGAGATGGCT 
AAATATGTCC AAGGAGATGC CATTGGGTAC GAGGGATTCC AGCAATTCCT GAAAATCTAT 
CTCGAAGTGG ATAATGTTCC CAGACACCTA AGCCTGGCAC TGTTTCAATC CTTTGAGACT 
GGTCACTGCT TAAATGAGAC AAATGTGACA AAAGATGTGG TGTGTCTCAA TGATGTTTCC 
TGCTACTTTT CCCTTCTGGA GGGTGGTCGG CCAGAAGACA AGTTAGAATT CACCTTCAAG 
CTGTACGACA CGGACAGAAA TGGGATCCTG GACAGCTCAG AAGTGGACAA AATTATCCTA 
CAGATGATGC GAGTGGCTGA ATACCTGGAT TGGGATGTGT CTGAGCTGAG GCCGATTCTT 
CAGGAGATGA TGAAAGAGAT TGACTATGAT GGCAGTGGCT CTGTCTCTCA AGCTGAGTGG 
GTCCGGGCTG GGGCCACCAC CGTGCCACTG CTAGTGCTGC TGGGTCTGGA GATGACTCTG 
AAGGACGACG GACAGCACAT GTGGAGGCCC AAGAGGTTCC CCAGACCAGT CTACTGCAAT 
CTGTGCGAGT CAAGCATTGG TCTTGGCAAA CAGGGACTGA GCTGTAACCT CTGTAAGTAC 
ACTGTTCACG ACCAGTGTGC CATGAAAGCC CTGCCTTGTG AAGTCAGCAC CTATGCCAAG 
TCTCGGAAGG ACATTGGTGT CCAATCACAT GTGTGGGTGC GAGGAGGCTG TGAGTCCGGG 
CGCTGCGACC GCTGTCAGAA AAAGATCCGG ATCTACCACA GTCTGACCGG GCTGCATTGT 
GTATGGTGCC ACCTAGAGAT CCACGATGAC TGCCTGCAAG CGGTGGGCCA TGAGTGTGAC 
TGTGGGCTGC TCCGGGATCA CATCCTGCCT CCATCTTCCA TCTATCCCAG TGTCCTGGCC 
TCTGGACCGG ATCGTAAAAA TAGCAAAACA AGCCAGAAGA CCATGGATGA TTTAAATTTG 
AGCACCTCTG AGGCTCTGCG GATTGACCCT GTTCCTAACA CCCACCCACT TCTCGTCTTT 
GTCAATCCTA AGAGTGGCGG GAAGCAGGGG CAAAGGGTGC TCTGGAAGTT CCAGTATATA 
TTAAACCCTC GACAGGTGTT CAACCTCCTA AAGGATGGTC CTGAGATAGG GCTCCGATTA 
TTCAAGGATG TTCCTGATAG CCGGATTTTG GTGTGTGGTG GAGACGGCAC AGTAGGCTGG 
ATTCTAGAGA CCATTGACAA AGCTAACTTG CCAGTTTTGC CTCCTGTTGC TGTGTTGCCC 
CTGGGTACTG GAAATGATCT GGCTCGATGC CTAAGATGGG GAGGAGGTTA TGAAGGACAG 
AATCTGGCAA AGATCCTCAA GGATTTAGAG ATGAGTAAAG TGGTACATAT GGATCGATGG 
TCTGTGGAGG TGATACCTCA ACAAACTGAA GAAAAAAGTG ACCCAGTCCC CTTTCAAATC 
ATCAATAACT ACTTCTCTAT TGGCGTGGAT GCCTCTATTG CTCATCGATT CCACATCATG 
CGAGAGAAAT ATCCGGAGAA GTTCAACAGC AGAATGAAGA ACAAGCTATG GTACTTCGAA 
TTTGCCACAT CTGAATCCAT CTTCTCAACA TGCAAAAAGC TGGAGGAGTC TTTGACAGTT 
GAGATCTGTG GGAAACCGCT GGATCTGAGC AACCTGTCCC TAGAAGGCAT CGCAGTGCTA 
AACATCCCTA GCATGCATGG TGGCTCCAAC CTCTGGGGTG ATACCAGGAG ACCCCATGGG 
GATATCTATG GGATCAACCA GGCCTTAGGT GCTACAGCTA AAGTCATCAC CGACCCTGAT 
ATCCTGAAAA CCTGTGTACC AGACCTAAGT GACAAGAGAC TGGAAGTGGT TGGGCTGGAG 
GGTGCAATTG AGATGGGCCA AATCTATACC AAGCTCAAGA ATGCTGGACG TCGGCTGGCC 
AAGTGCTCTG AGATCACCTT CCACACCACA AAAACCCTTC CCATGCAAAT TGACGGAGAA 
CCCTGGATGC AGACGCCCTG TACAATCAAG ATCACCCACA AGAACCAGAT GCCCATGCTC 
ATGGGCCCAC CCCCCCGCTC CACCAATTTC TTTGGCTTCT TGAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_958852.1
CDS	310..2517
Translation	MAKERGLISPSDFAQLQKYMEYSTKKVSDVLKLFEDGEMAKYVQGDAIGYEGFQQFLKIYLEVDNVPRHLSLALFQSFETGHCLNETNVTKDVVCLNDVSCYFSLLEGGRPEDKLEFTFKLYDTDRNGILDSSEVDKIILQMMRVAEYLDWDVSELRPILQEMMKEIDYDGSGSVSQAEWVRAGATTVPLLVLLGLEMTLKDDGQHMWRPKRFPRPVYCNLCESSIGLGKQGLSCNLCKYTVHDQCAMKALPCEVSTYAKSRKDIGVQSHVWVRGGCESGRCDRCQKKIRIYHSLTGLHCVWCHLEIHDDCLQAVGHECDCGLLRDHILPPSSIYPSVLASGPDRKNSKTSQKTMDDLNLSTSEALRIDPVPNTHPLLVFVNPKSGGKQGQRVLWKFQYILNPRQVFNLLKDGPEIGLRLFKDVPDSRILVCGGDGTVGWILETIDKANLPVLPPVAVLPLGTGNDLARCLRWGGGYEGQNLAKILKDLEMSKVVHMDRWSVEVIPQQTEEKSDPVPFQIINNYFSIGVDASIAHRFHIMREKYPEKFNSRMKNKLWYFEFATSESIFSTCKKLEESLTVEICGKPLDLSNLSLEGIAVLNIPSMHGGSNLWGDTRRPHGDIYGINQALGATAKVITDPDILKTCVPDLSDKRLEVVGLEGAIEMGQIYTKLKNAGRRLAKCSEITFHTTKTLPMQIDGEPWMQTPCTIKITHKNQMPMLMGPPPRSTNFFGFLS

Target ORF information:

RefSeq Version	NM_201444.2
Organism	Homo sapiens(human)
Definition	Homo sapiens diacylglycerol kinase alpha (DGKA), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_201444.2

ORF Insert Sequence:

ATGGCCAAGG AGAGGGGCCT AATAAGCCCC AGTGATTTTG CCCAGCTGCA AAAATACATG 
GAATACTCCA CCAAAAAGGT CAGTGATGTC CTAAAGCTCT TCGAGGATGG CGAGATGGCT 
AAATATGTCC AAGGAGATGC CATTGGGTAC GAGGGATTCC AGCAATTCCT GAAAATCTAT 
CTCGAAGTGG ATAATGTTCC CAGACACCTA AGCCTGGCAC TGTTTCAATC CTTTGAGACT 
GGTCACTGCT TAAATGAGAC AAATGTGACA AAAGATGTGG TGTGTCTCAA TGATGTTTCC 
TGCTACTTTT CCCTTCTGGA GGGTGGTCGG CCAGAAGACA AGTTAGAATT CACCTTCAAG 
CTGTACGACA CGGACAGAAA TGGGATCCTG GACAGCTCAG AAGTGGACAA AATTATCCTA 
CAGATGATGC GAGTGGCTGA ATACCTGGAT TGGGATGTGT CTGAGCTGAG GCCGATTCTT 
CAGGAGATGA TGAAAGAGAT TGACTATGAT GGCAGTGGCT CTGTCTCTCA AGCTGAGTGG 
GTCCGGGCTG GGGCCACCAC CGTGCCACTG CTAGTGCTGC TGGGTCTGGA GATGACTCTG 
AAGGACGACG GACAGCACAT GTGGAGGCCC AAGAGGTTCC CCAGACCAGT CTACTGCAAT 
CTGTGCGAGT CAAGCATTGG TCTTGGCAAA CAGGGACTGA GCTGTAACCT CTGTAAGTAC 
ACTGTTCACG ACCAGTGTGC CATGAAAGCC CTGCCTTGTG AAGTCAGCAC CTATGCCAAG 
TCTCGGAAGG ACATTGGTGT CCAATCACAT GTGTGGGTGC GAGGAGGCTG TGAGTCCGGG 
CGCTGCGACC GCTGTCAGAA AAAGATCCGG ATCTACCACA GTCTGACCGG GCTGCATTGT 
GTATGGTGCC ACCTAGAGAT CCACGATGAC TGCCTGCAAG CGGTGGGCCA TGAGTGTGAC 
TGTGGGCTGC TCCGGGATCA CATCCTGCCT CCATCTTCCA TCTATCCCAG TGTCCTGGCC 
TCTGGACCGG ATCGTAAAAA TAGCAAAACA AGCCAGAAGA CCATGGATGA TTTAAATTTG 
AGCACCTCTG AGGCTCTGCG GATTGACCCT GTTCCTAACA CCCACCCACT TCTCGTCTTT 
GTCAATCCTA AGAGTGGCGG GAAGCAGGGG CAAAGGGTGC TCTGGAAGTT CCAGTATATA 
TTAAACCCTC GACAGGTGTT CAACCTCCTA AAGGATGGTC CTGAGATAGG GCTCCGATTA 
TTCAAGGATG TTCCTGATAG CCGGATTTTG GTGTGTGGTG GAGACGGCAC AGTAGGCTGG 
ATTCTAGAGA CCATTGACAA AGCTAACTTG CCAGTTTTGC CTCCTGTTGC TGTGTTGCCC 
CTGGGTACTG GAAATGATCT GGCTCGATGC CTAAGATGGG GAGGAGGTTA TGAAGGACAG 
AATCTGGCAA AGATCCTCAA GGATTTAGAG ATGAGTAAAG TGGTACATAT GGATCGATGG 
TCTGTGGAGG TGATACCTCA ACAAACTGAA GAAAAAAGTG ACCCAGTCCC CTTTCAAATC 
ATCAATAACT ACTTCTCTAT TGGCGTGGAT GCCTCTATTG CTCATCGATT CCACATCATG 
CGAGAGAAAT ATCCGGAGAA GTTCAACAGC AGAATGAAGA ACAAGCTATG GTACTTCGAA 
TTTGCCACAT CTGAATCCAT CTTCTCAACA TGCAAAAAGC TGGAGGAGTC TTTGACAGTT 
GAGATCTGTG GGAAACCGCT GGATCTGAGC AACCTGTCCC TAGAAGGCAT CGCAGTGCTA 
AACATCCCTA GCATGCATGG TGGCTCCAAC CTCTGGGGTG ATACCAGGAG ACCCCATGGG 
GATATCTATG GGATCAACCA GGCCTTAGGT GCTACAGCTA AAGTCATCAC CGACCCTGAT 
ATCCTGAAAA CCTGTGTACC AGACCTAAGT GACAAGAGAC TGGAAGTGGT TGGGCTGGAG 
GGTGCAATTG AGATGGGCCA AATCTATACC AAGCTCAAGA ATGCTGGACG TCGGCTGGCC 
AAGTGCTCTG AGATCACCTT CCACACCACA AAAACCCTTC CCATGCAAAT TGACGGAGAA 
CCCTGGATGC AGACGCCCTG TACAATCAAG ATCACCCACA AGAACCAGAT GCCCATGCTC 
ATGGGCCCAC CCCCCCGCTC CACCAATTTC TTTGGCTTCT TGAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DGKA ( NM_001351034.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001337963.1 Homo sapiens diacylglycerol kinase alpha (DGKA), transcript variant 6, mRNA.

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CloneID	OHu02866
Clone ID Related Accession (Same CDS sequence)	NM_001351033.1 , NM_201445.1 , NM_201554.1 , NM_001345.4 , XM_011537992.1 , XM_011537991.1 , XM_011537993.1 , NM_001345.5 , NM_201444.2 , NM_001351034.1 , XM_011537993.2
Accession Version	NM_001351034.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2208bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-30
Organism	Homo sapiens(human)
Product	diacylglycerol kinase alpha isoform a
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC025162.48. On Apr 29, 2017 this sequence version replaced XM_011537991.2. Transcript Variant: This variant (6), as well as variants 1-5, encodes isoform a. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.274080.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCAAGG AGAGGGGCCT AATAAGCCCC AGTGATTTTG CCCAGCTGCA AAAATACATG 
GAATACTCCA CCAAAAAGGT CAGTGATGTC CTAAAGCTCT TCGAGGATGG CGAGATGGCT 
AAATATGTCC AAGGAGATGC CATTGGGTAC GAGGGATTCC AGCAATTCCT GAAAATCTAT 
CTCGAAGTGG ATAATGTTCC CAGACACCTA AGCCTGGCAC TGTTTCAATC CTTTGAGACT 
GGTCACTGCT TAAATGAGAC AAATGTGACA AAAGATGTGG TGTGTCTCAA TGATGTTTCC 
TGCTACTTTT CCCTTCTGGA GGGTGGTCGG CCAGAAGACA AGTTAGAATT CACCTTCAAG 
CTGTACGACA CGGACAGAAA TGGGATCCTG GACAGCTCAG AAGTGGACAA AATTATCCTA 
CAGATGATGC GAGTGGCTGA ATACCTGGAT TGGGATGTGT CTGAGCTGAG GCCGATTCTT 
CAGGAGATGA TGAAAGAGAT TGACTATGAT GGCAGTGGCT CTGTCTCTCA AGCTGAGTGG 
GTCCGGGCTG GGGCCACCAC CGTGCCACTG CTAGTGCTGC TGGGTCTGGA GATGACTCTG 
AAGGACGACG GACAGCACAT GTGGAGGCCC AAGAGGTTCC CCAGACCAGT CTACTGCAAT 
CTGTGCGAGT CAAGCATTGG TCTTGGCAAA CAGGGACTGA GCTGTAACCT CTGTAAGTAC 
ACTGTTCACG ACCAGTGTGC CATGAAAGCC CTGCCTTGTG AAGTCAGCAC CTATGCCAAG 
TCTCGGAAGG ACATTGGTGT CCAATCACAT GTGTGGGTGC GAGGAGGCTG TGAGTCCGGG 
CGCTGCGACC GCTGTCAGAA AAAGATCCGG ATCTACCACA GTCTGACCGG GCTGCATTGT 
GTATGGTGCC ACCTAGAGAT CCACGATGAC TGCCTGCAAG CGGTGGGCCA TGAGTGTGAC 
TGTGGGCTGC TCCGGGATCA CATCCTGCCT CCATCTTCCA TCTATCCCAG TGTCCTGGCC 
TCTGGACCGG ATCGTAAAAA TAGCAAAACA AGCCAGAAGA CCATGGATGA TTTAAATTTG 
AGCACCTCTG AGGCTCTGCG GATTGACCCT GTTCCTAACA CCCACCCACT TCTCGTCTTT 
GTCAATCCTA AGAGTGGCGG GAAGCAGGGG CAAAGGGTGC TCTGGAAGTT CCAGTATATA 
TTAAACCCTC GACAGGTGTT CAACCTCCTA AAGGATGGTC CTGAGATAGG GCTCCGATTA 
TTCAAGGATG TTCCTGATAG CCGGATTTTG GTGTGTGGTG GAGACGGCAC AGTAGGCTGG 
ATTCTAGAGA CCATTGACAA AGCTAACTTG CCAGTTTTGC CTCCTGTTGC TGTGTTGCCC 
CTGGGTACTG GAAATGATCT GGCTCGATGC CTAAGATGGG GAGGAGGTTA TGAAGGACAG 
AATCTGGCAA AGATCCTCAA GGATTTAGAG ATGAGTAAAG TGGTACATAT GGATCGATGG 
TCTGTGGAGG TGATACCTCA ACAAACTGAA GAAAAAAGTG ACCCAGTCCC CTTTCAAATC 
ATCAATAACT ACTTCTCTAT TGGCGTGGAT GCCTCTATTG CTCATCGATT CCACATCATG 
CGAGAGAAAT ATCCGGAGAA GTTCAACAGC AGAATGAAGA ACAAGCTATG GTACTTCGAA 
TTTGCCACAT CTGAATCCAT CTTCTCAACA TGCAAAAAGC TGGAGGAGTC TTTGACAGTT 
GAGATCTGTG GGAAACCGCT GGATCTGAGC AACCTGTCCC TAGAAGGCAT CGCAGTGCTA 
AACATCCCTA GCATGCATGG TGGCTCCAAC CTCTGGGGTG ATACCAGGAG ACCCCATGGG 
GATATCTATG GGATCAACCA GGCCTTAGGT GCTACAGCTA AAGTCATCAC CGACCCTGAT 
ATCCTGAAAA CCTGTGTACC AGACCTAAGT GACAAGAGAC TGGAAGTGGT TGGGCTGGAG 
GGTGCAATTG AGATGGGCCA AATCTATACC AAGCTCAAGA ATGCTGGACG TCGGCTGGCC 
AAGTGCTCTG AGATCACCTT CCACACCACA AAAACCCTTC CCATGCAAAT TGACGGAGAA 
CCCTGGATGC AGACGCCCTG TACAATCAAG ATCACCCACA AGAACCAGAT GCCCATGCTC 
ATGGGCCCAC CCCCCCGCTC CACCAATTTC TTTGGCTTCT TGAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001337963.1
CDS	410..2617
Translation	MAKERGLISPSDFAQLQKYMEYSTKKVSDVLKLFEDGEMAKYVQGDAIGYEGFQQFLKIYLEVDNVPRHLSLALFQSFETGHCLNETNVTKDVVCLNDVSCYFSLLEGGRPEDKLEFTFKLYDTDRNGILDSSEVDKIILQMMRVAEYLDWDVSELRPILQEMMKEIDYDGSGSVSQAEWVRAGATTVPLLVLLGLEMTLKDDGQHMWRPKRFPRPVYCNLCESSIGLGKQGLSCNLCKYTVHDQCAMKALPCEVSTYAKSRKDIGVQSHVWVRGGCESGRCDRCQKKIRIYHSLTGLHCVWCHLEIHDDCLQAVGHECDCGLLRDHILPPSSIYPSVLASGPDRKNSKTSQKTMDDLNLSTSEALRIDPVPNTHPLLVFVNPKSGGKQGQRVLWKFQYILNPRQVFNLLKDGPEIGLRLFKDVPDSRILVCGGDGTVGWILETIDKANLPVLPPVAVLPLGTGNDLARCLRWGGGYEGQNLAKILKDLEMSKVVHMDRWSVEVIPQQTEEKSDPVPFQIINNYFSIGVDASIAHRFHIMREKYPEKFNSRMKNKLWYFEFATSESIFSTCKKLEESLTVEICGKPLDLSNLSLEGIAVLNIPSMHGGSNLWGDTRRPHGDIYGINQALGATAKVITDPDILKTCVPDLSDKRLEVVGLEGAIEMGQIYTKLKNAGRRLAKCSEITFHTTKTLPMQIDGEPWMQTPCTIKITHKNQMPMLMGPPPRSTNFFGFLS

Target ORF information:

RefSeq Version	NM_001351034.1
Organism	Homo sapiens(human)
Definition	Homo sapiens diacylglycerol kinase alpha (DGKA), transcript variant 6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001351034.1

ORF Insert Sequence:

ATGGCCAAGG AGAGGGGCCT AATAAGCCCC AGTGATTTTG CCCAGCTGCA AAAATACATG 
GAATACTCCA CCAAAAAGGT CAGTGATGTC CTAAAGCTCT TCGAGGATGG CGAGATGGCT 
AAATATGTCC AAGGAGATGC CATTGGGTAC GAGGGATTCC AGCAATTCCT GAAAATCTAT 
CTCGAAGTGG ATAATGTTCC CAGACACCTA AGCCTGGCAC TGTTTCAATC CTTTGAGACT 
GGTCACTGCT TAAATGAGAC AAATGTGACA AAAGATGTGG TGTGTCTCAA TGATGTTTCC 
TGCTACTTTT CCCTTCTGGA GGGTGGTCGG CCAGAAGACA AGTTAGAATT CACCTTCAAG 
CTGTACGACA CGGACAGAAA TGGGATCCTG GACAGCTCAG AAGTGGACAA AATTATCCTA 
CAGATGATGC GAGTGGCTGA ATACCTGGAT TGGGATGTGT CTGAGCTGAG GCCGATTCTT 
CAGGAGATGA TGAAAGAGAT TGACTATGAT GGCAGTGGCT CTGTCTCTCA AGCTGAGTGG 
GTCCGGGCTG GGGCCACCAC CGTGCCACTG CTAGTGCTGC TGGGTCTGGA GATGACTCTG 
AAGGACGACG GACAGCACAT GTGGAGGCCC AAGAGGTTCC CCAGACCAGT CTACTGCAAT 
CTGTGCGAGT CAAGCATTGG TCTTGGCAAA CAGGGACTGA GCTGTAACCT CTGTAAGTAC 
ACTGTTCACG ACCAGTGTGC CATGAAAGCC CTGCCTTGTG AAGTCAGCAC CTATGCCAAG 
TCTCGGAAGG ACATTGGTGT CCAATCACAT GTGTGGGTGC GAGGAGGCTG TGAGTCCGGG 
CGCTGCGACC GCTGTCAGAA AAAGATCCGG ATCTACCACA GTCTGACCGG GCTGCATTGT 
GTATGGTGCC ACCTAGAGAT CCACGATGAC TGCCTGCAAG CGGTGGGCCA TGAGTGTGAC 
TGTGGGCTGC TCCGGGATCA CATCCTGCCT CCATCTTCCA TCTATCCCAG TGTCCTGGCC 
TCTGGACCGG ATCGTAAAAA TAGCAAAACA AGCCAGAAGA CCATGGATGA TTTAAATTTG 
AGCACCTCTG AGGCTCTGCG GATTGACCCT GTTCCTAACA CCCACCCACT TCTCGTCTTT 
GTCAATCCTA AGAGTGGCGG GAAGCAGGGG CAAAGGGTGC TCTGGAAGTT CCAGTATATA 
TTAAACCCTC GACAGGTGTT CAACCTCCTA AAGGATGGTC CTGAGATAGG GCTCCGATTA 
TTCAAGGATG TTCCTGATAG CCGGATTTTG GTGTGTGGTG GAGACGGCAC AGTAGGCTGG 
ATTCTAGAGA CCATTGACAA AGCTAACTTG CCAGTTTTGC CTCCTGTTGC TGTGTTGCCC 
CTGGGTACTG GAAATGATCT GGCTCGATGC CTAAGATGGG GAGGAGGTTA TGAAGGACAG 
AATCTGGCAA AGATCCTCAA GGATTTAGAG ATGAGTAAAG TGGTACATAT GGATCGATGG 
TCTGTGGAGG TGATACCTCA ACAAACTGAA GAAAAAAGTG ACCCAGTCCC CTTTCAAATC 
ATCAATAACT ACTTCTCTAT TGGCGTGGAT GCCTCTATTG CTCATCGATT CCACATCATG 
CGAGAGAAAT ATCCGGAGAA GTTCAACAGC AGAATGAAGA ACAAGCTATG GTACTTCGAA 
TTTGCCACAT CTGAATCCAT CTTCTCAACA TGCAAAAAGC TGGAGGAGTC TTTGACAGTT 
GAGATCTGTG GGAAACCGCT GGATCTGAGC AACCTGTCCC TAGAAGGCAT CGCAGTGCTA 
AACATCCCTA GCATGCATGG TGGCTCCAAC CTCTGGGGTG ATACCAGGAG ACCCCATGGG 
GATATCTATG GGATCAACCA GGCCTTAGGT GCTACAGCTA AAGTCATCAC CGACCCTGAT 
ATCCTGAAAA CCTGTGTACC AGACCTAAGT GACAAGAGAC TGGAAGTGGT TGGGCTGGAG 
GGTGCAATTG AGATGGGCCA AATCTATACC AAGCTCAAGA ATGCTGGACG TCGGCTGGCC 
AAGTGCTCTG AGATCACCTT CCACACCACA AAAACCCTTC CCATGCAAAT TGACGGAGAA 
CCCTGGATGC AGACGCCCTG TACAATCAAG ATCACCCACA AGAACCAGAT GCCCATGCTC 
ATGGGCCCAC CCCCCCGCTC CACCAATTTC TTTGGCTTCT TGAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DGKA ( XM_011537992.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011536294.1 Homo sapiens diacylglycerol kinase, alpha 80kDa (DGKA), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OHu02866
Clone ID Related Accession (Same CDS sequence)	NM_001351033.1 , NM_201445.1 , NM_201554.1 , NM_001345.4 , XM_011537992.1 , XM_011537991.1 , XM_011537993.1 , NM_001345.5 , NM_201444.2 , NM_001351034.1 , XM_011537993.2
Accession Version	XM_011537992.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2208bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-11
Organism	Homo sapiens(Human)
Product	diacylglycerol kinase alpha isoform X1
Comment

ATGGCCAAGG AGAGGGGCCT AATAAGCCCC AGTGATTTTG CCCAGCTGCA AAAATACATG 
GAATACTCCA CCAAAAAGGT CAGTGATGTC CTAAAGCTCT TCGAGGATGG CGAGATGGCT 
AAATATGTCC AAGGAGATGC CATTGGGTAC GAGGGATTCC AGCAATTCCT GAAAATCTAT 
CTCGAAGTGG ATAATGTTCC CAGACACCTA AGCCTGGCAC TGTTTCAATC CTTTGAGACT 
GGTCACTGCT TAAATGAGAC AAATGTGACA AAAGATGTGG TGTGTCTCAA TGATGTTTCC 
TGCTACTTTT CCCTTCTGGA GGGTGGTCGG CCAGAAGACA AGTTAGAATT CACCTTCAAG 
CTGTACGACA CGGACAGAAA TGGGATCCTG GACAGCTCAG AAGTGGACAA AATTATCCTA 
CAGATGATGC GAGTGGCTGA ATACCTGGAT TGGGATGTGT CTGAGCTGAG GCCGATTCTT 
CAGGAGATGA TGAAAGAGAT TGACTATGAT GGCAGTGGCT CTGTCTCTCA AGCTGAGTGG 
GTCCGGGCTG GGGCCACCAC CGTGCCACTG CTAGTGCTGC TGGGTCTGGA GATGACTCTG 
AAGGACGACG GACAGCACAT GTGGAGGCCC AAGAGGTTCC CCAGACCAGT CTACTGCAAT 
CTGTGCGAGT CAAGCATTGG TCTTGGCAAA CAGGGACTGA GCTGTAACCT CTGTAAGTAC 
ACTGTTCACG ACCAGTGTGC CATGAAAGCC CTGCCTTGTG AAGTCAGCAC CTATGCCAAG 
TCTCGGAAGG ACATTGGTGT CCAATCACAT GTGTGGGTGC GAGGAGGCTG TGAGTCCGGG 
CGCTGCGACC GCTGTCAGAA AAAGATCCGG ATCTACCACA GTCTGACCGG GCTGCATTGT 
GTATGGTGCC ACCTAGAGAT CCACGATGAC TGCCTGCAAG CGGTGGGCCA TGAGTGTGAC 
TGTGGGCTGC TCCGGGATCA CATCCTGCCT CCATCTTCCA TCTATCCCAG TGTCCTGGCC 
TCTGGACCGG ATCGTAAAAA TAGCAAAACA AGCCAGAAGA CCATGGATGA TTTAAATTTG 
AGCACCTCTG AGGCTCTGCG GATTGACCCT GTTCCTAACA CCCACCCACT TCTCGTCTTT 
GTCAATCCTA AGAGTGGCGG GAAGCAGGGG CAAAGGGTGC TCTGGAAGTT CCAGTATATA 
TTAAACCCTC GACAGGTGTT CAACCTCCTA AAGGATGGTC CTGAGATAGG GCTCCGATTA 
TTCAAGGATG TTCCTGATAG CCGGATTTTG GTGTGTGGTG GAGACGGCAC AGTAGGCTGG 
ATTCTAGAGA CCATTGACAA AGCTAACTTG CCAGTTTTGC CTCCTGTTGC TGTGTTGCCC 
CTGGGTACTG GAAATGATCT GGCTCGATGC CTAAGATGGG GAGGAGGTTA TGAAGGACAG 
AATCTGGCAA AGATCCTCAA GGATTTAGAG ATGAGTAAAG TGGTACATAT GGATCGATGG 
TCTGTGGAGG TGATACCTCA ACAAACTGAA GAAAAAAGTG ACCCAGTCCC CTTTCAAATC 
ATCAATAACT ACTTCTCTAT TGGCGTGGAT GCCTCTATTG CTCATCGATT CCACATCATG 
CGAGAGAAAT ATCCGGAGAA GTTCAACAGC AGAATGAAGA ACAAGCTATG GTACTTCGAA 
TTTGCCACAT CTGAATCCAT CTTCTCAACA TGCAAAAAGC TGGAGGAGTC TTTGACAGTT 
GAGATCTGTG GGAAACCGCT GGATCTGAGC AACCTGTCCC TAGAAGGCAT CGCAGTGCTA 
AACATCCCTA GCATGCATGG TGGCTCCAAC CTCTGGGGTG ATACCAGGAG ACCCCATGGG 
GATATCTATG GGATCAACCA GGCCTTAGGT GCTACAGCTA AAGTCATCAC CGACCCTGAT 
ATCCTGAAAA CCTGTGTACC AGACCTAAGT GACAAGAGAC TGGAAGTGGT TGGGCTGGAG 
GGTGCAATTG AGATGGGCCA AATCTATACC AAGCTCAAGA ATGCTGGACG TCGGCTGGCC 
AAGTGCTCTG AGATCACCTT CCACACCACA AAAACCCTTC CCATGCAAAT TGACGGAGAA 
CCCTGGATGC AGACGCCCTG TACAATCAAG ATCACCCACA AGAACCAGAT GCCCATGCTC 
ATGGGCCCAC CCCCCCGCTC CACCAATTTC TTTGGCTTCT TGAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

XP_011536294.1

Translation

MAKERGLISPSDFAQLQKYMEYSTKKVSDVLKLFEDGEMAKYVQGDAIGYEGFQQFLKIYLEVDNVPRHLSLALFQSFETGHCLNETNVTKDVVCLNDVSCYFSLLEGGRPEDKLEFTFKLYDTDRNGILDSSEVDKIILQMMRVAEYLDWDVSELRPILQEMMKEIDYDGSGSVSQAEWVRAGATTVPLLVLLGLEMTLKDDGQHMWRPKRFPRPVYCNLCESSIGLGKQGLSCNLCKYTVHDQCAMKALPCEVSTYAKSRKDIGVQSHVWVRGGCESGRCDRCQKKIRIYHSLTGLHCVWCHLEIHDDCLQAVGHECDCGLLRDHILPPSSIYPSVLASGPDRKNSKTSQKTMDDLNLSTSEALRIDPVPNTHPLLVFVNPKSGGKQGQRVLWKFQYILNPRQVFNLLKDGPEIGLRLFKDVPDSRILVCGGDGTVGWILETIDKANLPVLPPVAVLPLGTGNDLARCLRWGGGYEGQNLAKILKDLEMSKVVHMDRWSVEVIPQQTEEKSDPVPFQIINNYFSIGVDASIAHRFHIMREKYPEKFNSRMKNKLWYFEFATSESIFSTCKKLEESLTVEICGKPLDLSNLSLEGIAVLNIPSMHGGSNLWGDTRRPHGDIYGINQALGATAKVITDPDILKTCVPDLSDKRLEVVGLEGAIEMGQIYTKLKNAGRRLAKCSEITFHTTKTLPMQIDGEPWMQTPCTIKITHKNQMPMLMGPPPRSTNFFGFLS

Target ORF information:

RefSeq Version	XM_011537992.1
Organism	Homo sapiens(Human)
Definition	Homo sapiens diacylglycerol kinase, alpha 80kDa (DGKA), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011537992.1

ORF Insert Sequence:

ATGGCCAAGG AGAGGGGCCT AATAAGCCCC AGTGATTTTG CCCAGCTGCA AAAATACATG 
GAATACTCCA CCAAAAAGGT CAGTGATGTC CTAAAGCTCT TCGAGGATGG CGAGATGGCT 
AAATATGTCC AAGGAGATGC CATTGGGTAC GAGGGATTCC AGCAATTCCT GAAAATCTAT 
CTCGAAGTGG ATAATGTTCC CAGACACCTA AGCCTGGCAC TGTTTCAATC CTTTGAGACT 
GGTCACTGCT TAAATGAGAC AAATGTGACA AAAGATGTGG TGTGTCTCAA TGATGTTTCC 
TGCTACTTTT CCCTTCTGGA GGGTGGTCGG CCAGAAGACA AGTTAGAATT CACCTTCAAG 
CTGTACGACA CGGACAGAAA TGGGATCCTG GACAGCTCAG AAGTGGACAA AATTATCCTA 
CAGATGATGC GAGTGGCTGA ATACCTGGAT TGGGATGTGT CTGAGCTGAG GCCGATTCTT 
CAGGAGATGA TGAAAGAGAT TGACTATGAT GGCAGTGGCT CTGTCTCTCA AGCTGAGTGG 
GTCCGGGCTG GGGCCACCAC CGTGCCACTG CTAGTGCTGC TGGGTCTGGA GATGACTCTG 
AAGGACGACG GACAGCACAT GTGGAGGCCC AAGAGGTTCC CCAGACCAGT CTACTGCAAT 
CTGTGCGAGT CAAGCATTGG TCTTGGCAAA CAGGGACTGA GCTGTAACCT CTGTAAGTAC 
ACTGTTCACG ACCAGTGTGC CATGAAAGCC CTGCCTTGTG AAGTCAGCAC CTATGCCAAG 
TCTCGGAAGG ACATTGGTGT CCAATCACAT GTGTGGGTGC GAGGAGGCTG TGAGTCCGGG 
CGCTGCGACC GCTGTCAGAA AAAGATCCGG ATCTACCACA GTCTGACCGG GCTGCATTGT 
GTATGGTGCC ACCTAGAGAT CCACGATGAC TGCCTGCAAG CGGTGGGCCA TGAGTGTGAC 
TGTGGGCTGC TCCGGGATCA CATCCTGCCT CCATCTTCCA TCTATCCCAG TGTCCTGGCC 
TCTGGACCGG ATCGTAAAAA TAGCAAAACA AGCCAGAAGA CCATGGATGA TTTAAATTTG 
AGCACCTCTG AGGCTCTGCG GATTGACCCT GTTCCTAACA CCCACCCACT TCTCGTCTTT 
GTCAATCCTA AGAGTGGCGG GAAGCAGGGG CAAAGGGTGC TCTGGAAGTT CCAGTATATA 
TTAAACCCTC GACAGGTGTT CAACCTCCTA AAGGATGGTC CTGAGATAGG GCTCCGATTA 
TTCAAGGATG TTCCTGATAG CCGGATTTTG GTGTGTGGTG GAGACGGCAC AGTAGGCTGG 
ATTCTAGAGA CCATTGACAA AGCTAACTTG CCAGTTTTGC CTCCTGTTGC TGTGTTGCCC 
CTGGGTACTG GAAATGATCT GGCTCGATGC CTAAGATGGG GAGGAGGTTA TGAAGGACAG 
AATCTGGCAA AGATCCTCAA GGATTTAGAG ATGAGTAAAG TGGTACATAT GGATCGATGG 
TCTGTGGAGG TGATACCTCA ACAAACTGAA GAAAAAAGTG ACCCAGTCCC CTTTCAAATC 
ATCAATAACT ACTTCTCTAT TGGCGTGGAT GCCTCTATTG CTCATCGATT CCACATCATG 
CGAGAGAAAT ATCCGGAGAA GTTCAACAGC AGAATGAAGA ACAAGCTATG GTACTTCGAA 
TTTGCCACAT CTGAATCCAT CTTCTCAACA TGCAAAAAGC TGGAGGAGTC TTTGACAGTT 
GAGATCTGTG GGAAACCGCT GGATCTGAGC AACCTGTCCC TAGAAGGCAT CGCAGTGCTA 
AACATCCCTA GCATGCATGG TGGCTCCAAC CTCTGGGGTG ATACCAGGAG ACCCCATGGG 
GATATCTATG GGATCAACCA GGCCTTAGGT GCTACAGCTA AAGTCATCAC CGACCCTGAT 
ATCCTGAAAA CCTGTGTACC AGACCTAAGT GACAAGAGAC TGGAAGTGGT TGGGCTGGAG 
GGTGCAATTG AGATGGGCCA AATCTATACC AAGCTCAAGA ATGCTGGACG TCGGCTGGCC 
AAGTGCTCTG AGATCACCTT CCACACCACA AAAACCCTTC CCATGCAAAT TGACGGAGAA 
CCCTGGATGC AGACGCCCTG TACAATCAAG ATCACCCACA AGAACCAGAT GCCCATGCTC 
ATGGGCCCAC CCCCCCGCTC CACCAATTTC TTTGGCTTCT TGAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DGKA ( XM_011537991.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011536293.1 Homo sapiens diacylglycerol kinase, alpha 80kDa (DGKA), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHu02866
Clone ID Related Accession (Same CDS sequence)	NM_001351033.1 , NM_201445.1 , NM_201554.1 , NM_001345.4 , XM_011537992.1 , XM_011537991.1 , XM_011537993.1 , NM_001345.5 , NM_201444.2 , NM_001351034.1 , XM_011537993.2
Accession Version	XM_011537991.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2208bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-11
Organism	Homo sapiens(Human)
Product	diacylglycerol kinase alpha isoform X1
Comment

ATGGCCAAGG AGAGGGGCCT AATAAGCCCC AGTGATTTTG CCCAGCTGCA AAAATACATG 
GAATACTCCA CCAAAAAGGT CAGTGATGTC CTAAAGCTCT TCGAGGATGG CGAGATGGCT 
AAATATGTCC AAGGAGATGC CATTGGGTAC GAGGGATTCC AGCAATTCCT GAAAATCTAT 
CTCGAAGTGG ATAATGTTCC CAGACACCTA AGCCTGGCAC TGTTTCAATC CTTTGAGACT 
GGTCACTGCT TAAATGAGAC AAATGTGACA AAAGATGTGG TGTGTCTCAA TGATGTTTCC 
TGCTACTTTT CCCTTCTGGA GGGTGGTCGG CCAGAAGACA AGTTAGAATT CACCTTCAAG 
CTGTACGACA CGGACAGAAA TGGGATCCTG GACAGCTCAG AAGTGGACAA AATTATCCTA 
CAGATGATGC GAGTGGCTGA ATACCTGGAT TGGGATGTGT CTGAGCTGAG GCCGATTCTT 
CAGGAGATGA TGAAAGAGAT TGACTATGAT GGCAGTGGCT CTGTCTCTCA AGCTGAGTGG 
GTCCGGGCTG GGGCCACCAC CGTGCCACTG CTAGTGCTGC TGGGTCTGGA GATGACTCTG 
AAGGACGACG GACAGCACAT GTGGAGGCCC AAGAGGTTCC CCAGACCAGT CTACTGCAAT 
CTGTGCGAGT CAAGCATTGG TCTTGGCAAA CAGGGACTGA GCTGTAACCT CTGTAAGTAC 
ACTGTTCACG ACCAGTGTGC CATGAAAGCC CTGCCTTGTG AAGTCAGCAC CTATGCCAAG 
TCTCGGAAGG ACATTGGTGT CCAATCACAT GTGTGGGTGC GAGGAGGCTG TGAGTCCGGG 
CGCTGCGACC GCTGTCAGAA AAAGATCCGG ATCTACCACA GTCTGACCGG GCTGCATTGT 
GTATGGTGCC ACCTAGAGAT CCACGATGAC TGCCTGCAAG CGGTGGGCCA TGAGTGTGAC 
TGTGGGCTGC TCCGGGATCA CATCCTGCCT CCATCTTCCA TCTATCCCAG TGTCCTGGCC 
TCTGGACCGG ATCGTAAAAA TAGCAAAACA AGCCAGAAGA CCATGGATGA TTTAAATTTG 
AGCACCTCTG AGGCTCTGCG GATTGACCCT GTTCCTAACA CCCACCCACT TCTCGTCTTT 
GTCAATCCTA AGAGTGGCGG GAAGCAGGGG CAAAGGGTGC TCTGGAAGTT CCAGTATATA 
TTAAACCCTC GACAGGTGTT CAACCTCCTA AAGGATGGTC CTGAGATAGG GCTCCGATTA 
TTCAAGGATG TTCCTGATAG CCGGATTTTG GTGTGTGGTG GAGACGGCAC AGTAGGCTGG 
ATTCTAGAGA CCATTGACAA AGCTAACTTG CCAGTTTTGC CTCCTGTTGC TGTGTTGCCC 
CTGGGTACTG GAAATGATCT GGCTCGATGC CTAAGATGGG GAGGAGGTTA TGAAGGACAG 
AATCTGGCAA AGATCCTCAA GGATTTAGAG ATGAGTAAAG TGGTACATAT GGATCGATGG 
TCTGTGGAGG TGATACCTCA ACAAACTGAA GAAAAAAGTG ACCCAGTCCC CTTTCAAATC 
ATCAATAACT ACTTCTCTAT TGGCGTGGAT GCCTCTATTG CTCATCGATT CCACATCATG 
CGAGAGAAAT ATCCGGAGAA GTTCAACAGC AGAATGAAGA ACAAGCTATG GTACTTCGAA 
TTTGCCACAT CTGAATCCAT CTTCTCAACA TGCAAAAAGC TGGAGGAGTC TTTGACAGTT 
GAGATCTGTG GGAAACCGCT GGATCTGAGC AACCTGTCCC TAGAAGGCAT CGCAGTGCTA 
AACATCCCTA GCATGCATGG TGGCTCCAAC CTCTGGGGTG ATACCAGGAG ACCCCATGGG 
GATATCTATG GGATCAACCA GGCCTTAGGT GCTACAGCTA AAGTCATCAC CGACCCTGAT 
ATCCTGAAAA CCTGTGTACC AGACCTAAGT GACAAGAGAC TGGAAGTGGT TGGGCTGGAG 
GGTGCAATTG AGATGGGCCA AATCTATACC AAGCTCAAGA ATGCTGGACG TCGGCTGGCC 
AAGTGCTCTG AGATCACCTT CCACACCACA AAAACCCTTC CCATGCAAAT TGACGGAGAA 
CCCTGGATGC AGACGCCCTG TACAATCAAG ATCACCCACA AGAACCAGAT GCCCATGCTC 
ATGGGCCCAC CCCCCCGCTC CACCAATTTC TTTGGCTTCT TGAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

XP_011536293.1

Translation

MAKERGLISPSDFAQLQKYMEYSTKKVSDVLKLFEDGEMAKYVQGDAIGYEGFQQFLKIYLEVDNVPRHLSLALFQSFETGHCLNETNVTKDVVCLNDVSCYFSLLEGGRPEDKLEFTFKLYDTDRNGILDSSEVDKIILQMMRVAEYLDWDVSELRPILQEMMKEIDYDGSGSVSQAEWVRAGATTVPLLVLLGLEMTLKDDGQHMWRPKRFPRPVYCNLCESSIGLGKQGLSCNLCKYTVHDQCAMKALPCEVSTYAKSRKDIGVQSHVWVRGGCESGRCDRCQKKIRIYHSLTGLHCVWCHLEIHDDCLQAVGHECDCGLLRDHILPPSSIYPSVLASGPDRKNSKTSQKTMDDLNLSTSEALRIDPVPNTHPLLVFVNPKSGGKQGQRVLWKFQYILNPRQVFNLLKDGPEIGLRLFKDVPDSRILVCGGDGTVGWILETIDKANLPVLPPVAVLPLGTGNDLARCLRWGGGYEGQNLAKILKDLEMSKVVHMDRWSVEVIPQQTEEKSDPVPFQIINNYFSIGVDASIAHRFHIMREKYPEKFNSRMKNKLWYFEFATSESIFSTCKKLEESLTVEICGKPLDLSNLSLEGIAVLNIPSMHGGSNLWGDTRRPHGDIYGINQALGATAKVITDPDILKTCVPDLSDKRLEVVGLEGAIEMGQIYTKLKNAGRRLAKCSEITFHTTKTLPMQIDGEPWMQTPCTIKITHKNQMPMLMGPPPRSTNFFGFLS

Target ORF information:

RefSeq Version	XM_011537991.1
Organism	Homo sapiens(Human)
Definition	Homo sapiens diacylglycerol kinase, alpha 80kDa (DGKA), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011537991.1

ORF Insert Sequence:

ATGGCCAAGG AGAGGGGCCT AATAAGCCCC AGTGATTTTG CCCAGCTGCA AAAATACATG 
GAATACTCCA CCAAAAAGGT CAGTGATGTC CTAAAGCTCT TCGAGGATGG CGAGATGGCT 
AAATATGTCC AAGGAGATGC CATTGGGTAC GAGGGATTCC AGCAATTCCT GAAAATCTAT 
CTCGAAGTGG ATAATGTTCC CAGACACCTA AGCCTGGCAC TGTTTCAATC CTTTGAGACT 
GGTCACTGCT TAAATGAGAC AAATGTGACA AAAGATGTGG TGTGTCTCAA TGATGTTTCC 
TGCTACTTTT CCCTTCTGGA GGGTGGTCGG CCAGAAGACA AGTTAGAATT CACCTTCAAG 
CTGTACGACA CGGACAGAAA TGGGATCCTG GACAGCTCAG AAGTGGACAA AATTATCCTA 
CAGATGATGC GAGTGGCTGA ATACCTGGAT TGGGATGTGT CTGAGCTGAG GCCGATTCTT 
CAGGAGATGA TGAAAGAGAT TGACTATGAT GGCAGTGGCT CTGTCTCTCA AGCTGAGTGG 
GTCCGGGCTG GGGCCACCAC CGTGCCACTG CTAGTGCTGC TGGGTCTGGA GATGACTCTG 
AAGGACGACG GACAGCACAT GTGGAGGCCC AAGAGGTTCC CCAGACCAGT CTACTGCAAT 
CTGTGCGAGT CAAGCATTGG TCTTGGCAAA CAGGGACTGA GCTGTAACCT CTGTAAGTAC 
ACTGTTCACG ACCAGTGTGC CATGAAAGCC CTGCCTTGTG AAGTCAGCAC CTATGCCAAG 
TCTCGGAAGG ACATTGGTGT CCAATCACAT GTGTGGGTGC GAGGAGGCTG TGAGTCCGGG 
CGCTGCGACC GCTGTCAGAA AAAGATCCGG ATCTACCACA GTCTGACCGG GCTGCATTGT 
GTATGGTGCC ACCTAGAGAT CCACGATGAC TGCCTGCAAG CGGTGGGCCA TGAGTGTGAC 
TGTGGGCTGC TCCGGGATCA CATCCTGCCT CCATCTTCCA TCTATCCCAG TGTCCTGGCC 
TCTGGACCGG ATCGTAAAAA TAGCAAAACA AGCCAGAAGA CCATGGATGA TTTAAATTTG 
AGCACCTCTG AGGCTCTGCG GATTGACCCT GTTCCTAACA CCCACCCACT TCTCGTCTTT 
GTCAATCCTA AGAGTGGCGG GAAGCAGGGG CAAAGGGTGC TCTGGAAGTT CCAGTATATA 
TTAAACCCTC GACAGGTGTT CAACCTCCTA AAGGATGGTC CTGAGATAGG GCTCCGATTA 
TTCAAGGATG TTCCTGATAG CCGGATTTTG GTGTGTGGTG GAGACGGCAC AGTAGGCTGG 
ATTCTAGAGA CCATTGACAA AGCTAACTTG CCAGTTTTGC CTCCTGTTGC TGTGTTGCCC 
CTGGGTACTG GAAATGATCT GGCTCGATGC CTAAGATGGG GAGGAGGTTA TGAAGGACAG 
AATCTGGCAA AGATCCTCAA GGATTTAGAG ATGAGTAAAG TGGTACATAT GGATCGATGG 
TCTGTGGAGG TGATACCTCA ACAAACTGAA GAAAAAAGTG ACCCAGTCCC CTTTCAAATC 
ATCAATAACT ACTTCTCTAT TGGCGTGGAT GCCTCTATTG CTCATCGATT CCACATCATG 
CGAGAGAAAT ATCCGGAGAA GTTCAACAGC AGAATGAAGA ACAAGCTATG GTACTTCGAA 
TTTGCCACAT CTGAATCCAT CTTCTCAACA TGCAAAAAGC TGGAGGAGTC TTTGACAGTT 
GAGATCTGTG GGAAACCGCT GGATCTGAGC AACCTGTCCC TAGAAGGCAT CGCAGTGCTA 
AACATCCCTA GCATGCATGG TGGCTCCAAC CTCTGGGGTG ATACCAGGAG ACCCCATGGG 
GATATCTATG GGATCAACCA GGCCTTAGGT GCTACAGCTA AAGTCATCAC CGACCCTGAT 
ATCCTGAAAA CCTGTGTACC AGACCTAAGT GACAAGAGAC TGGAAGTGGT TGGGCTGGAG 
GGTGCAATTG AGATGGGCCA AATCTATACC AAGCTCAAGA ATGCTGGACG TCGGCTGGCC 
AAGTGCTCTG AGATCACCTT CCACACCACA AAAACCCTTC CCATGCAAAT TGACGGAGAA 
CCCTGGATGC AGACGCCCTG TACAATCAAG ATCACCCACA AGAACCAGAT GCCCATGCTC 
ATGGGCCCAC CCCCCCGCTC CACCAATTTC TTTGGCTTCT TGAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DGKA ( XM_011537993.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011536295.1 Homo sapiens diacylglycerol kinase, alpha 80kDa (DGKA), transcript variant X5, mRNA.

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CloneID	OHu02866
Clone ID Related Accession (Same CDS sequence)	NM_001351033.1 , NM_201445.1 , NM_201554.1 , NM_001345.4 , XM_011537992.1 , XM_011537991.1 , XM_011537993.1 , NM_001345.5 , NM_201444.2 , NM_001351034.1 , XM_011537993.2
Accession Version	XM_011537993.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2208bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-11
Organism	Homo sapiens(Human)
Product	diacylglycerol kinase alpha isoform X1
Comment

ATGGCCAAGG AGAGGGGCCT AATAAGCCCC AGTGATTTTG CCCAGCTGCA AAAATACATG 
GAATACTCCA CCAAAAAGGT CAGTGATGTC CTAAAGCTCT TCGAGGATGG CGAGATGGCT 
AAATATGTCC AAGGAGATGC CATTGGGTAC GAGGGATTCC AGCAATTCCT GAAAATCTAT 
CTCGAAGTGG ATAATGTTCC CAGACACCTA AGCCTGGCAC TGTTTCAATC CTTTGAGACT 
GGTCACTGCT TAAATGAGAC AAATGTGACA AAAGATGTGG TGTGTCTCAA TGATGTTTCC 
TGCTACTTTT CCCTTCTGGA GGGTGGTCGG CCAGAAGACA AGTTAGAATT CACCTTCAAG 
CTGTACGACA CGGACAGAAA TGGGATCCTG GACAGCTCAG AAGTGGACAA AATTATCCTA 
CAGATGATGC GAGTGGCTGA ATACCTGGAT TGGGATGTGT CTGAGCTGAG GCCGATTCTT 
CAGGAGATGA TGAAAGAGAT TGACTATGAT GGCAGTGGCT CTGTCTCTCA AGCTGAGTGG 
GTCCGGGCTG GGGCCACCAC CGTGCCACTG CTAGTGCTGC TGGGTCTGGA GATGACTCTG 
AAGGACGACG GACAGCACAT GTGGAGGCCC AAGAGGTTCC CCAGACCAGT CTACTGCAAT 
CTGTGCGAGT CAAGCATTGG TCTTGGCAAA CAGGGACTGA GCTGTAACCT CTGTAAGTAC 
ACTGTTCACG ACCAGTGTGC CATGAAAGCC CTGCCTTGTG AAGTCAGCAC CTATGCCAAG 
TCTCGGAAGG ACATTGGTGT CCAATCACAT GTGTGGGTGC GAGGAGGCTG TGAGTCCGGG 
CGCTGCGACC GCTGTCAGAA AAAGATCCGG ATCTACCACA GTCTGACCGG GCTGCATTGT 
GTATGGTGCC ACCTAGAGAT CCACGATGAC TGCCTGCAAG CGGTGGGCCA TGAGTGTGAC 
TGTGGGCTGC TCCGGGATCA CATCCTGCCT CCATCTTCCA TCTATCCCAG TGTCCTGGCC 
TCTGGACCGG ATCGTAAAAA TAGCAAAACA AGCCAGAAGA CCATGGATGA TTTAAATTTG 
AGCACCTCTG AGGCTCTGCG GATTGACCCT GTTCCTAACA CCCACCCACT TCTCGTCTTT 
GTCAATCCTA AGAGTGGCGG GAAGCAGGGG CAAAGGGTGC TCTGGAAGTT CCAGTATATA 
TTAAACCCTC GACAGGTGTT CAACCTCCTA AAGGATGGTC CTGAGATAGG GCTCCGATTA 
TTCAAGGATG TTCCTGATAG CCGGATTTTG GTGTGTGGTG GAGACGGCAC AGTAGGCTGG 
ATTCTAGAGA CCATTGACAA AGCTAACTTG CCAGTTTTGC CTCCTGTTGC TGTGTTGCCC 
CTGGGTACTG GAAATGATCT GGCTCGATGC CTAAGATGGG GAGGAGGTTA TGAAGGACAG 
AATCTGGCAA AGATCCTCAA GGATTTAGAG ATGAGTAAAG TGGTACATAT GGATCGATGG 
TCTGTGGAGG TGATACCTCA ACAAACTGAA GAAAAAAGTG ACCCAGTCCC CTTTCAAATC 
ATCAATAACT ACTTCTCTAT TGGCGTGGAT GCCTCTATTG CTCATCGATT CCACATCATG 
CGAGAGAAAT ATCCGGAGAA GTTCAACAGC AGAATGAAGA ACAAGCTATG GTACTTCGAA 
TTTGCCACAT CTGAATCCAT CTTCTCAACA TGCAAAAAGC TGGAGGAGTC TTTGACAGTT 
GAGATCTGTG GGAAACCGCT GGATCTGAGC AACCTGTCCC TAGAAGGCAT CGCAGTGCTA 
AACATCCCTA GCATGCATGG TGGCTCCAAC CTCTGGGGTG ATACCAGGAG ACCCCATGGG 
GATATCTATG GGATCAACCA GGCCTTAGGT GCTACAGCTA AAGTCATCAC CGACCCTGAT 
ATCCTGAAAA CCTGTGTACC AGACCTAAGT GACAAGAGAC TGGAAGTGGT TGGGCTGGAG 
GGTGCAATTG AGATGGGCCA AATCTATACC AAGCTCAAGA ATGCTGGACG TCGGCTGGCC 
AAGTGCTCTG AGATCACCTT CCACACCACA AAAACCCTTC CCATGCAAAT TGACGGAGAA 
CCCTGGATGC AGACGCCCTG TACAATCAAG ATCACCCACA AGAACCAGAT GCCCATGCTC 
ATGGGCCCAC CCCCCCGCTC CACCAATTTC TTTGGCTTCT TGAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

XP_011536295.1

Translation

MAKERGLISPSDFAQLQKYMEYSTKKVSDVLKLFEDGEMAKYVQGDAIGYEGFQQFLKIYLEVDNVPRHLSLALFQSFETGHCLNETNVTKDVVCLNDVSCYFSLLEGGRPEDKLEFTFKLYDTDRNGILDSSEVDKIILQMMRVAEYLDWDVSELRPILQEMMKEIDYDGSGSVSQAEWVRAGATTVPLLVLLGLEMTLKDDGQHMWRPKRFPRPVYCNLCESSIGLGKQGLSCNLCKYTVHDQCAMKALPCEVSTYAKSRKDIGVQSHVWVRGGCESGRCDRCQKKIRIYHSLTGLHCVWCHLEIHDDCLQAVGHECDCGLLRDHILPPSSIYPSVLASGPDRKNSKTSQKTMDDLNLSTSEALRIDPVPNTHPLLVFVNPKSGGKQGQRVLWKFQYILNPRQVFNLLKDGPEIGLRLFKDVPDSRILVCGGDGTVGWILETIDKANLPVLPPVAVLPLGTGNDLARCLRWGGGYEGQNLAKILKDLEMSKVVHMDRWSVEVIPQQTEEKSDPVPFQIINNYFSIGVDASIAHRFHIMREKYPEKFNSRMKNKLWYFEFATSESIFSTCKKLEESLTVEICGKPLDLSNLSLEGIAVLNIPSMHGGSNLWGDTRRPHGDIYGINQALGATAKVITDPDILKTCVPDLSDKRLEVVGLEGAIEMGQIYTKLKNAGRRLAKCSEITFHTTKTLPMQIDGEPWMQTPCTIKITHKNQMPMLMGPPPRSTNFFGFLS

Target ORF information:

RefSeq Version	XM_011537993.1
Organism	Homo sapiens(Human)
Definition	Homo sapiens diacylglycerol kinase, alpha 80kDa (DGKA), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011537993.1

ORF Insert Sequence:

ATGGCCAAGG AGAGGGGCCT AATAAGCCCC AGTGATTTTG CCCAGCTGCA AAAATACATG 
GAATACTCCA CCAAAAAGGT CAGTGATGTC CTAAAGCTCT TCGAGGATGG CGAGATGGCT 
AAATATGTCC AAGGAGATGC CATTGGGTAC GAGGGATTCC AGCAATTCCT GAAAATCTAT 
CTCGAAGTGG ATAATGTTCC CAGACACCTA AGCCTGGCAC TGTTTCAATC CTTTGAGACT 
GGTCACTGCT TAAATGAGAC AAATGTGACA AAAGATGTGG TGTGTCTCAA TGATGTTTCC 
TGCTACTTTT CCCTTCTGGA GGGTGGTCGG CCAGAAGACA AGTTAGAATT CACCTTCAAG 
CTGTACGACA CGGACAGAAA TGGGATCCTG GACAGCTCAG AAGTGGACAA AATTATCCTA 
CAGATGATGC GAGTGGCTGA ATACCTGGAT TGGGATGTGT CTGAGCTGAG GCCGATTCTT 
CAGGAGATGA TGAAAGAGAT TGACTATGAT GGCAGTGGCT CTGTCTCTCA AGCTGAGTGG 
GTCCGGGCTG GGGCCACCAC CGTGCCACTG CTAGTGCTGC TGGGTCTGGA GATGACTCTG 
AAGGACGACG GACAGCACAT GTGGAGGCCC AAGAGGTTCC CCAGACCAGT CTACTGCAAT 
CTGTGCGAGT CAAGCATTGG TCTTGGCAAA CAGGGACTGA GCTGTAACCT CTGTAAGTAC 
ACTGTTCACG ACCAGTGTGC CATGAAAGCC CTGCCTTGTG AAGTCAGCAC CTATGCCAAG 
TCTCGGAAGG ACATTGGTGT CCAATCACAT GTGTGGGTGC GAGGAGGCTG TGAGTCCGGG 
CGCTGCGACC GCTGTCAGAA AAAGATCCGG ATCTACCACA GTCTGACCGG GCTGCATTGT 
GTATGGTGCC ACCTAGAGAT CCACGATGAC TGCCTGCAAG CGGTGGGCCA TGAGTGTGAC 
TGTGGGCTGC TCCGGGATCA CATCCTGCCT CCATCTTCCA TCTATCCCAG TGTCCTGGCC 
TCTGGACCGG ATCGTAAAAA TAGCAAAACA AGCCAGAAGA CCATGGATGA TTTAAATTTG 
AGCACCTCTG AGGCTCTGCG GATTGACCCT GTTCCTAACA CCCACCCACT TCTCGTCTTT 
GTCAATCCTA AGAGTGGCGG GAAGCAGGGG CAAAGGGTGC TCTGGAAGTT CCAGTATATA 
TTAAACCCTC GACAGGTGTT CAACCTCCTA AAGGATGGTC CTGAGATAGG GCTCCGATTA 
TTCAAGGATG TTCCTGATAG CCGGATTTTG GTGTGTGGTG GAGACGGCAC AGTAGGCTGG 
ATTCTAGAGA CCATTGACAA AGCTAACTTG CCAGTTTTGC CTCCTGTTGC TGTGTTGCCC 
CTGGGTACTG GAAATGATCT GGCTCGATGC CTAAGATGGG GAGGAGGTTA TGAAGGACAG 
AATCTGGCAA AGATCCTCAA GGATTTAGAG ATGAGTAAAG TGGTACATAT GGATCGATGG 
TCTGTGGAGG TGATACCTCA ACAAACTGAA GAAAAAAGTG ACCCAGTCCC CTTTCAAATC 
ATCAATAACT ACTTCTCTAT TGGCGTGGAT GCCTCTATTG CTCATCGATT CCACATCATG 
CGAGAGAAAT ATCCGGAGAA GTTCAACAGC AGAATGAAGA ACAAGCTATG GTACTTCGAA 
TTTGCCACAT CTGAATCCAT CTTCTCAACA TGCAAAAAGC TGGAGGAGTC TTTGACAGTT 
GAGATCTGTG GGAAACCGCT GGATCTGAGC AACCTGTCCC TAGAAGGCAT CGCAGTGCTA 
AACATCCCTA GCATGCATGG TGGCTCCAAC CTCTGGGGTG ATACCAGGAG ACCCCATGGG 
GATATCTATG GGATCAACCA GGCCTTAGGT GCTACAGCTA AAGTCATCAC CGACCCTGAT 
ATCCTGAAAA CCTGTGTACC AGACCTAAGT GACAAGAGAC TGGAAGTGGT TGGGCTGGAG 
GGTGCAATTG AGATGGGCCA AATCTATACC AAGCTCAAGA ATGCTGGACG TCGGCTGGCC 
AAGTGCTCTG AGATCACCTT CCACACCACA AAAACCCTTC CCATGCAAAT TGACGGAGAA 
CCCTGGATGC AGACGCCCTG TACAATCAAG ATCACCCACA AGAACCAGAT GCCCATGCTC 
ATGGGCCCAC CCCCCCGCTC CACCAATTTC TTTGGCTTCT TGAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DGKA ( XM_011537993.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011536295.1 Homo sapiens diacylglycerol kinase alpha (DGKA), transcript variant X10, mRNA.

Price & Availability↑

CloneID	OHu02866
Clone ID Related Accession (Same CDS sequence)	NM_001351033.1 , NM_201445.1 , NM_201554.1 , NM_001345.4 , XM_011537992.1 , XM_011537991.1 , XM_011537993.1 , NM_001345.5 , NM_201444.2 , NM_001351034.1 , XM_011537993.2
Accession Version	XM_011537993.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2208bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	diacylglycerol kinase alpha isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011537993.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAGG AGAGGGGCCT AATAAGCCCC AGTGATTTTG CCCAGCTGCA AAAATACATG 
GAATACTCCA CCAAAAAGGT CAGTGATGTC CTAAAGCTCT TCGAGGATGG CGAGATGGCT 
AAATATGTCC AAGGAGATGC CATTGGGTAC GAGGGATTCC AGCAATTCCT GAAAATCTAT 
CTCGAAGTGG ATAATGTTCC CAGACACCTA AGCCTGGCAC TGTTTCAATC CTTTGAGACT 
GGTCACTGCT TAAATGAGAC AAATGTGACA AAAGATGTGG TGTGTCTCAA TGATGTTTCC 
TGCTACTTTT CCCTTCTGGA GGGTGGTCGG CCAGAAGACA AGTTAGAATT CACCTTCAAG 
CTGTACGACA CGGACAGAAA TGGGATCCTG GACAGCTCAG AAGTGGACAA AATTATCCTA 
CAGATGATGC GAGTGGCTGA ATACCTGGAT TGGGATGTGT CTGAGCTGAG GCCGATTCTT 
CAGGAGATGA TGAAAGAGAT TGACTATGAT GGCAGTGGCT CTGTCTCTCA AGCTGAGTGG 
GTCCGGGCTG GGGCCACCAC CGTGCCACTG CTAGTGCTGC TGGGTCTGGA GATGACTCTG 
AAGGACGACG GACAGCACAT GTGGAGGCCC AAGAGGTTCC CCAGACCAGT CTACTGCAAT 
CTGTGCGAGT CAAGCATTGG TCTTGGCAAA CAGGGACTGA GCTGTAACCT CTGTAAGTAC 
ACTGTTCACG ACCAGTGTGC CATGAAAGCC CTGCCTTGTG AAGTCAGCAC CTATGCCAAG 
TCTCGGAAGG ACATTGGTGT CCAATCACAT GTGTGGGTGC GAGGAGGCTG TGAGTCCGGG 
CGCTGCGACC GCTGTCAGAA AAAGATCCGG ATCTACCACA GTCTGACCGG GCTGCATTGT 
GTATGGTGCC ACCTAGAGAT CCACGATGAC TGCCTGCAAG CGGTGGGCCA TGAGTGTGAC 
TGTGGGCTGC TCCGGGATCA CATCCTGCCT CCATCTTCCA TCTATCCCAG TGTCCTGGCC 
TCTGGACCGG ATCGTAAAAA TAGCAAAACA AGCCAGAAGA CCATGGATGA TTTAAATTTG 
AGCACCTCTG AGGCTCTGCG GATTGACCCT GTTCCTAACA CCCACCCACT TCTCGTCTTT 
GTCAATCCTA AGAGTGGCGG GAAGCAGGGG CAAAGGGTGC TCTGGAAGTT CCAGTATATA 
TTAAACCCTC GACAGGTGTT CAACCTCCTA AAGGATGGTC CTGAGATAGG GCTCCGATTA 
TTCAAGGATG TTCCTGATAG CCGGATTTTG GTGTGTGGTG GAGACGGCAC AGTAGGCTGG 
ATTCTAGAGA CCATTGACAA AGCTAACTTG CCAGTTTTGC CTCCTGTTGC TGTGTTGCCC 
CTGGGTACTG GAAATGATCT GGCTCGATGC CTAAGATGGG GAGGAGGTTA TGAAGGACAG 
AATCTGGCAA AGATCCTCAA GGATTTAGAG ATGAGTAAAG TGGTACATAT GGATCGATGG 
TCTGTGGAGG TGATACCTCA ACAAACTGAA GAAAAAAGTG ACCCAGTCCC CTTTCAAATC 
ATCAATAACT ACTTCTCTAT TGGCGTGGAT GCCTCTATTG CTCATCGATT CCACATCATG 
CGAGAGAAAT ATCCGGAGAA GTTCAACAGC AGAATGAAGA ACAAGCTATG GTACTTCGAA 
TTTGCCACAT CTGAATCCAT CTTCTCAACA TGCAAAAAGC TGGAGGAGTC TTTGACAGTT 
GAGATCTGTG GGAAACCGCT GGATCTGAGC AACCTGTCCC TAGAAGGCAT CGCAGTGCTA 
AACATCCCTA GCATGCATGG TGGCTCCAAC CTCTGGGGTG ATACCAGGAG ACCCCATGGG 
GATATCTATG GGATCAACCA GGCCTTAGGT GCTACAGCTA AAGTCATCAC CGACCCTGAT 
ATCCTGAAAA CCTGTGTACC AGACCTAAGT GACAAGAGAC TGGAAGTGGT TGGGCTGGAG 
GGTGCAATTG AGATGGGCCA AATCTATACC AAGCTCAAGA ATGCTGGACG TCGGCTGGCC 
AAGTGCTCTG AGATCACCTT CCACACCACA AAAACCCTTC CCATGCAAAT TGACGGAGAA 
CCCTGGATGC AGACGCCCTG TACAATCAAG ATCACCCACA AGAACCAGAT GCCCATGCTC 
ATGGGCCCAC CCCCCCGCTC CACCAATTTC TTTGGCTTCT TGAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011536295.1
CDS	126..2333
Translation	MAKERGLISPSDFAQLQKYMEYSTKKVSDVLKLFEDGEMAKYVQGDAIGYEGFQQFLKIYLEVDNVPRHLSLALFQSFETGHCLNETNVTKDVVCLNDVSCYFSLLEGGRPEDKLEFTFKLYDTDRNGILDSSEVDKIILQMMRVAEYLDWDVSELRPILQEMMKEIDYDGSGSVSQAEWVRAGATTVPLLVLLGLEMTLKDDGQHMWRPKRFPRPVYCNLCESSIGLGKQGLSCNLCKYTVHDQCAMKALPCEVSTYAKSRKDIGVQSHVWVRGGCESGRCDRCQKKIRIYHSLTGLHCVWCHLEIHDDCLQAVGHECDCGLLRDHILPPSSIYPSVLASGPDRKNSKTSQKTMDDLNLSTSEALRIDPVPNTHPLLVFVNPKSGGKQGQRVLWKFQYILNPRQVFNLLKDGPEIGLRLFKDVPDSRILVCGGDGTVGWILETIDKANLPVLPPVAVLPLGTGNDLARCLRWGGGYEGQNLAKILKDLEMSKVVHMDRWSVEVIPQQTEEKSDPVPFQIINNYFSIGVDASIAHRFHIMREKYPEKFNSRMKNKLWYFEFATSESIFSTCKKLEESLTVEICGKPLDLSNLSLEGIAVLNIPSMHGGSNLWGDTRRPHGDIYGINQALGATAKVITDPDILKTCVPDLSDKRLEVVGLEGAIEMGQIYTKLKNAGRRLAKCSEITFHTTKTLPMQIDGEPWMQTPCTIKITHKNQMPMLMGPPPRSTNFFGFLS

Target ORF information:

RefSeq Version	XM_011537993.2
Organism	Homo sapiens(human)
Definition	Homo sapiens diacylglycerol kinase alpha (DGKA), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011537993.2

ORF Insert Sequence:

ATGGCCAAGG AGAGGGGCCT AATAAGCCCC AGTGATTTTG CCCAGCTGCA AAAATACATG 
GAATACTCCA CCAAAAAGGT CAGTGATGTC CTAAAGCTCT TCGAGGATGG CGAGATGGCT 
AAATATGTCC AAGGAGATGC CATTGGGTAC GAGGGATTCC AGCAATTCCT GAAAATCTAT 
CTCGAAGTGG ATAATGTTCC CAGACACCTA AGCCTGGCAC TGTTTCAATC CTTTGAGACT 
GGTCACTGCT TAAATGAGAC AAATGTGACA AAAGATGTGG TGTGTCTCAA TGATGTTTCC 
TGCTACTTTT CCCTTCTGGA GGGTGGTCGG CCAGAAGACA AGTTAGAATT CACCTTCAAG 
CTGTACGACA CGGACAGAAA TGGGATCCTG GACAGCTCAG AAGTGGACAA AATTATCCTA 
CAGATGATGC GAGTGGCTGA ATACCTGGAT TGGGATGTGT CTGAGCTGAG GCCGATTCTT 
CAGGAGATGA TGAAAGAGAT TGACTATGAT GGCAGTGGCT CTGTCTCTCA AGCTGAGTGG 
GTCCGGGCTG GGGCCACCAC CGTGCCACTG CTAGTGCTGC TGGGTCTGGA GATGACTCTG 
AAGGACGACG GACAGCACAT GTGGAGGCCC AAGAGGTTCC CCAGACCAGT CTACTGCAAT 
CTGTGCGAGT CAAGCATTGG TCTTGGCAAA CAGGGACTGA GCTGTAACCT CTGTAAGTAC 
ACTGTTCACG ACCAGTGTGC CATGAAAGCC CTGCCTTGTG AAGTCAGCAC CTATGCCAAG 
TCTCGGAAGG ACATTGGTGT CCAATCACAT GTGTGGGTGC GAGGAGGCTG TGAGTCCGGG 
CGCTGCGACC GCTGTCAGAA AAAGATCCGG ATCTACCACA GTCTGACCGG GCTGCATTGT 
GTATGGTGCC ACCTAGAGAT CCACGATGAC TGCCTGCAAG CGGTGGGCCA TGAGTGTGAC 
TGTGGGCTGC TCCGGGATCA CATCCTGCCT CCATCTTCCA TCTATCCCAG TGTCCTGGCC 
TCTGGACCGG ATCGTAAAAA TAGCAAAACA AGCCAGAAGA CCATGGATGA TTTAAATTTG 
AGCACCTCTG AGGCTCTGCG GATTGACCCT GTTCCTAACA CCCACCCACT TCTCGTCTTT 
GTCAATCCTA AGAGTGGCGG GAAGCAGGGG CAAAGGGTGC TCTGGAAGTT CCAGTATATA 
TTAAACCCTC GACAGGTGTT CAACCTCCTA AAGGATGGTC CTGAGATAGG GCTCCGATTA 
TTCAAGGATG TTCCTGATAG CCGGATTTTG GTGTGTGGTG GAGACGGCAC AGTAGGCTGG 
ATTCTAGAGA CCATTGACAA AGCTAACTTG CCAGTTTTGC CTCCTGTTGC TGTGTTGCCC 
CTGGGTACTG GAAATGATCT GGCTCGATGC CTAAGATGGG GAGGAGGTTA TGAAGGACAG 
AATCTGGCAA AGATCCTCAA GGATTTAGAG ATGAGTAAAG TGGTACATAT GGATCGATGG 
TCTGTGGAGG TGATACCTCA ACAAACTGAA GAAAAAAGTG ACCCAGTCCC CTTTCAAATC 
ATCAATAACT ACTTCTCTAT TGGCGTGGAT GCCTCTATTG CTCATCGATT CCACATCATG 
CGAGAGAAAT ATCCGGAGAA GTTCAACAGC AGAATGAAGA ACAAGCTATG GTACTTCGAA 
TTTGCCACAT CTGAATCCAT CTTCTCAACA TGCAAAAAGC TGGAGGAGTC TTTGACAGTT 
GAGATCTGTG GGAAACCGCT GGATCTGAGC AACCTGTCCC TAGAAGGCAT CGCAGTGCTA 
AACATCCCTA GCATGCATGG TGGCTCCAAC CTCTGGGGTG ATACCAGGAG ACCCCATGGG 
GATATCTATG GGATCAACCA GGCCTTAGGT GCTACAGCTA AAGTCATCAC CGACCCTGAT 
ATCCTGAAAA CCTGTGTACC AGACCTAAGT GACAAGAGAC TGGAAGTGGT TGGGCTGGAG 
GGTGCAATTG AGATGGGCCA AATCTATACC AAGCTCAAGA ATGCTGGACG TCGGCTGGCC 
AAGTGCTCTG AGATCACCTT CCACACCACA AAAACCCTTC CCATGCAAAT TGACGGAGAA 
CCCTGGATGC AGACGCCCTG TACAATCAAG ATCACCCACA AGAACCAGAT GCCCATGCTC 
ATGGGCCCAC CCCCCCGCTC CACCAATTTC TTTGGCTTCT TGAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DGKA ( NM_001351038.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001337967.1 Homo sapiens diacylglycerol kinase alpha (DGKA), transcript variant 10, mRNA.

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CloneID	OHu102466
Clone ID Related Accession (Same CDS sequence)	NM_001351038.1 , NM_001351039.1 , NM_001351040.1
Accession Version	NM_001351038.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1146bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-30
Organism	Homo sapiens(human)
Product	diacylglycerol kinase alpha isoform d
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC025162.48. Transcript Variant: This variant (10), as well as variants 11 and 12, encodes isoform d. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY930112.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2153031, SAMEA2156670 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGATGATT TAAATTTGAG CACCTCTGAG GCTCTGCGGA TTGACCCTGT TCCTAACACC 
CACCCACTTC TCGTCTTTGT CAATCCTAAG AGTGGCGGGA AGCAGGGGCA AAGGGTGCTC 
TGGAAGTTCC AGTATATATT AAACCCTCGA CAGGTGTTCA ACCTCCTAAA GGATGGTCCT 
GAGATAGGGC TCCGATTATT CAAGGATGTT CCTGATAGCC GGATTTTGGT GTGTGGTGGA 
GACGGCACAG TAGGCTGGAT TCTAGAGACC ATTGACAAAG CTAACTTGCC AGTTTTGCCT 
CCTGTTGCTG TGTTGCCCCT GGGTACTGGA AATGATCTGG CTCGATGCCT AAGATGGGGA 
GGAGGTTATG AAGGACAGAA TCTGGCAAAG ATCCTCAAGG ATTTAGAGAT GAGTAAAGTG 
GTACATATGG ATCGATGGTC TGTGGAGGTG ATACCTCAAC AAACTGAAGA AAAAAGTGAC 
CCAGTCCCCT TTCAAATCAT CAATAACTAC TTCTCTATTG GCGTGGATGC CTCTATTGCT 
CATCGATTCC ACATCATGCG AGAGAAATAT CCGGAGAAGT TCAACAGCAG AATGAAGAAC 
AAGCTATGGT ACTTCGAATT TGCCACATCT GAATCCATCT TCTCAACATG CAAAAAGCTG 
GAGGAGTCTT TGACAGTTGA GATCTGTGGG AAACCGCTGG ATCTGAGCAA CCTGTCCCTA 
GAAGGCATCG CAGTGCTAAA CATCCCTAGC ATGCATGGTG GCTCCAACCT CTGGGGTGAT 
ACCAGGAGAC CCCATGGGGA TATCTATGGG ATCAACCAGG CCTTAGGTGC TACAGCTAAA 
GTCATCACCG ACCCTGATAT CCTGAAAACC TGTGTACCAG ACCTAAGTGA CAAGAGACTG 
GAAGTGGTTG GGCTGGAGGG TGCAATTGAG ATGGGCCAAA TCTATACCAA GCTCAAGAAT 
GCTGGACGTC GGCTGGCCAA GTGCTCTGAG ATCACCTTCC ACACCACAAA AACCCTTCCC 
ATGCAAATTG ACGGAGAACC CTGGATGCAG ACGCCCTGTA CAATCAAGAT CACCCACAAG 
AACCAGATGC CCATGCTCAT GGGCCCACCC CCCCGCTCCA CCAATTTCTT TGGCTTCTTG 
AGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001337967.1
CDS	1208..2353
Translation	MDDLNLSTSEALRIDPVPNTHPLLVFVNPKSGGKQGQRVLWKFQYILNPRQVFNLLKDGPEIGLRLFKDVPDSRILVCGGDGTVGWILETIDKANLPVLPPVAVLPLGTGNDLARCLRWGGGYEGQNLAKILKDLEMSKVVHMDRWSVEVIPQQTEEKSDPVPFQIINNYFSIGVDASIAHRFHIMREKYPEKFNSRMKNKLWYFEFATSESIFSTCKKLEESLTVEICGKPLDLSNLSLEGIAVLNIPSMHGGSNLWGDTRRPHGDIYGINQALGATAKVITDPDILKTCVPDLSDKRLEVVGLEGAIEMGQIYTKLKNAGRRLAKCSEITFHTTKTLPMQIDGEPWMQTPCTIKITHKNQMPMLMGPPPRSTNFFGFLS

Target ORF information:

RefSeq Version	NM_001351038.1
Organism	Homo sapiens(human)
Definition	Homo sapiens diacylglycerol kinase alpha (DGKA), transcript variant 10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001351038.1

ORF Insert Sequence:

ATGGATGATT TAAATTTGAG CACCTCTGAG GCTCTGCGGA TTGACCCTGT TCCTAACACC 
CACCCACTTC TCGTCTTTGT CAATCCTAAG AGTGGCGGGA AGCAGGGGCA AAGGGTGCTC 
TGGAAGTTCC AGTATATATT AAACCCTCGA CAGGTGTTCA ACCTCCTAAA GGATGGTCCT 
GAGATAGGGC TCCGATTATT CAAGGATGTT CCTGATAGCC GGATTTTGGT GTGTGGTGGA 
GACGGCACAG TAGGCTGGAT TCTAGAGACC ATTGACAAAG CTAACTTGCC AGTTTTGCCT 
CCTGTTGCTG TGTTGCCCCT GGGTACTGGA AATGATCTGG CTCGATGCCT AAGATGGGGA 
GGAGGTTATG AAGGACAGAA TCTGGCAAAG ATCCTCAAGG ATTTAGAGAT GAGTAAAGTG 
GTACATATGG ATCGATGGTC TGTGGAGGTG ATACCTCAAC AAACTGAAGA AAAAAGTGAC 
CCAGTCCCCT TTCAAATCAT CAATAACTAC TTCTCTATTG GCGTGGATGC CTCTATTGCT 
CATCGATTCC ACATCATGCG AGAGAAATAT CCGGAGAAGT TCAACAGCAG AATGAAGAAC 
AAGCTATGGT ACTTCGAATT TGCCACATCT GAATCCATCT TCTCAACATG CAAAAAGCTG 
GAGGAGTCTT TGACAGTTGA GATCTGTGGG AAACCGCTGG ATCTGAGCAA CCTGTCCCTA 
GAAGGCATCG CAGTGCTAAA CATCCCTAGC ATGCATGGTG GCTCCAACCT CTGGGGTGAT 
ACCAGGAGAC CCCATGGGGA TATCTATGGG ATCAACCAGG CCTTAGGTGC TACAGCTAAA 
GTCATCACCG ACCCTGATAT CCTGAAAACC TGTGTACCAG ACCTAAGTGA CAAGAGACTG 
GAAGTGGTTG GGCTGGAGGG TGCAATTGAG ATGGGCCAAA TCTATACCAA GCTCAAGAAT 
GCTGGACGTC GGCTGGCCAA GTGCTCTGAG ATCACCTTCC ACACCACAAA AACCCTTCCC 
ATGCAAATTG ACGGAGAACC CTGGATGCAG ACGCCCTGTA CAATCAAGAT CACCCACAAG 
AACCAGATGC CCATGCTCAT GGGCCCACCC CCCCGCTCCA CCAATTTCTT TGGCTTCTTG 
AGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DGKA ( NM_001351039.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001337968.1 Homo sapiens diacylglycerol kinase alpha (DGKA), transcript variant 11, mRNA.

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CloneID	OHu102466
Clone ID Related Accession (Same CDS sequence)	NM_001351038.1 , NM_001351039.1 , NM_001351040.1
Accession Version	NM_001351039.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1146bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-30
Organism	Homo sapiens(human)
Product	diacylglycerol kinase alpha isoform d
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC025162.48. Transcript Variant: This variant (11), as well as variants 10 and 12, encodes isoform d. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.86215.1, SRR1660803.84262.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGATGATT TAAATTTGAG CACCTCTGAG GCTCTGCGGA TTGACCCTGT TCCTAACACC 
CACCCACTTC TCGTCTTTGT CAATCCTAAG AGTGGCGGGA AGCAGGGGCA AAGGGTGCTC 
TGGAAGTTCC AGTATATATT AAACCCTCGA CAGGTGTTCA ACCTCCTAAA GGATGGTCCT 
GAGATAGGGC TCCGATTATT CAAGGATGTT CCTGATAGCC GGATTTTGGT GTGTGGTGGA 
GACGGCACAG TAGGCTGGAT TCTAGAGACC ATTGACAAAG CTAACTTGCC AGTTTTGCCT 
CCTGTTGCTG TGTTGCCCCT GGGTACTGGA AATGATCTGG CTCGATGCCT AAGATGGGGA 
GGAGGTTATG AAGGACAGAA TCTGGCAAAG ATCCTCAAGG ATTTAGAGAT GAGTAAAGTG 
GTACATATGG ATCGATGGTC TGTGGAGGTG ATACCTCAAC AAACTGAAGA AAAAAGTGAC 
CCAGTCCCCT TTCAAATCAT CAATAACTAC TTCTCTATTG GCGTGGATGC CTCTATTGCT 
CATCGATTCC ACATCATGCG AGAGAAATAT CCGGAGAAGT TCAACAGCAG AATGAAGAAC 
AAGCTATGGT ACTTCGAATT TGCCACATCT GAATCCATCT TCTCAACATG CAAAAAGCTG 
GAGGAGTCTT TGACAGTTGA GATCTGTGGG AAACCGCTGG ATCTGAGCAA CCTGTCCCTA 
GAAGGCATCG CAGTGCTAAA CATCCCTAGC ATGCATGGTG GCTCCAACCT CTGGGGTGAT 
ACCAGGAGAC CCCATGGGGA TATCTATGGG ATCAACCAGG CCTTAGGTGC TACAGCTAAA 
GTCATCACCG ACCCTGATAT CCTGAAAACC TGTGTACCAG ACCTAAGTGA CAAGAGACTG 
GAAGTGGTTG GGCTGGAGGG TGCAATTGAG ATGGGCCAAA TCTATACCAA GCTCAAGAAT 
GCTGGACGTC GGCTGGCCAA GTGCTCTGAG ATCACCTTCC ACACCACAAA AACCCTTCCC 
ATGCAAATTG ACGGAGAACC CTGGATGCAG ACGCCCTGTA CAATCAAGAT CACCCACAAG 
AACCAGATGC CCATGCTCAT GGGCCCACCC CCCCGCTCCA CCAATTTCTT TGGCTTCTTG 
AGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001337968.1
CDS	1151..2296
Translation	MDDLNLSTSEALRIDPVPNTHPLLVFVNPKSGGKQGQRVLWKFQYILNPRQVFNLLKDGPEIGLRLFKDVPDSRILVCGGDGTVGWILETIDKANLPVLPPVAVLPLGTGNDLARCLRWGGGYEGQNLAKILKDLEMSKVVHMDRWSVEVIPQQTEEKSDPVPFQIINNYFSIGVDASIAHRFHIMREKYPEKFNSRMKNKLWYFEFATSESIFSTCKKLEESLTVEICGKPLDLSNLSLEGIAVLNIPSMHGGSNLWGDTRRPHGDIYGINQALGATAKVITDPDILKTCVPDLSDKRLEVVGLEGAIEMGQIYTKLKNAGRRLAKCSEITFHTTKTLPMQIDGEPWMQTPCTIKITHKNQMPMLMGPPPRSTNFFGFLS

Target ORF information:

RefSeq Version	NM_001351039.1
Organism	Homo sapiens(human)
Definition	Homo sapiens diacylglycerol kinase alpha (DGKA), transcript variant 11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001351039.1

ORF Insert Sequence:

ATGGATGATT TAAATTTGAG CACCTCTGAG GCTCTGCGGA TTGACCCTGT TCCTAACACC 
CACCCACTTC TCGTCTTTGT CAATCCTAAG AGTGGCGGGA AGCAGGGGCA AAGGGTGCTC 
TGGAAGTTCC AGTATATATT AAACCCTCGA CAGGTGTTCA ACCTCCTAAA GGATGGTCCT 
GAGATAGGGC TCCGATTATT CAAGGATGTT CCTGATAGCC GGATTTTGGT GTGTGGTGGA 
GACGGCACAG TAGGCTGGAT TCTAGAGACC ATTGACAAAG CTAACTTGCC AGTTTTGCCT 
CCTGTTGCTG TGTTGCCCCT GGGTACTGGA AATGATCTGG CTCGATGCCT AAGATGGGGA 
GGAGGTTATG AAGGACAGAA TCTGGCAAAG ATCCTCAAGG ATTTAGAGAT GAGTAAAGTG 
GTACATATGG ATCGATGGTC TGTGGAGGTG ATACCTCAAC AAACTGAAGA AAAAAGTGAC 
CCAGTCCCCT TTCAAATCAT CAATAACTAC TTCTCTATTG GCGTGGATGC CTCTATTGCT 
CATCGATTCC ACATCATGCG AGAGAAATAT CCGGAGAAGT TCAACAGCAG AATGAAGAAC 
AAGCTATGGT ACTTCGAATT TGCCACATCT GAATCCATCT TCTCAACATG CAAAAAGCTG 
GAGGAGTCTT TGACAGTTGA GATCTGTGGG AAACCGCTGG ATCTGAGCAA CCTGTCCCTA 
GAAGGCATCG CAGTGCTAAA CATCCCTAGC ATGCATGGTG GCTCCAACCT CTGGGGTGAT 
ACCAGGAGAC CCCATGGGGA TATCTATGGG ATCAACCAGG CCTTAGGTGC TACAGCTAAA 
GTCATCACCG ACCCTGATAT CCTGAAAACC TGTGTACCAG ACCTAAGTGA CAAGAGACTG 
GAAGTGGTTG GGCTGGAGGG TGCAATTGAG ATGGGCCAAA TCTATACCAA GCTCAAGAAT 
GCTGGACGTC GGCTGGCCAA GTGCTCTGAG ATCACCTTCC ACACCACAAA AACCCTTCCC 
ATGCAAATTG ACGGAGAACC CTGGATGCAG ACGCCCTGTA CAATCAAGAT CACCCACAAG 
AACCAGATGC CCATGCTCAT GGGCCCACCC CCCCGCTCCA CCAATTTCTT TGGCTTCTTG 
AGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DGKA ( NM_001351040.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001337969.1 Homo sapiens diacylglycerol kinase alpha (DGKA), transcript variant 12, mRNA.

Price & Availability↑

CloneID	OHu102466
Clone ID Related Accession (Same CDS sequence)	NM_001351038.1 , NM_001351039.1 , NM_001351040.1
Accession Version	NM_001351040.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1146bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-30
Organism	Homo sapiens(human)
Product	diacylglycerol kinase alpha isoform d
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC025162.48. Transcript Variant: This variant (12), as well as variants 10 and 11, encodes isoform d. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.273309.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGATGATT TAAATTTGAG CACCTCTGAG GCTCTGCGGA TTGACCCTGT TCCTAACACC 
CACCCACTTC TCGTCTTTGT CAATCCTAAG AGTGGCGGGA AGCAGGGGCA AAGGGTGCTC 
TGGAAGTTCC AGTATATATT AAACCCTCGA CAGGTGTTCA ACCTCCTAAA GGATGGTCCT 
GAGATAGGGC TCCGATTATT CAAGGATGTT CCTGATAGCC GGATTTTGGT GTGTGGTGGA 
GACGGCACAG TAGGCTGGAT TCTAGAGACC ATTGACAAAG CTAACTTGCC AGTTTTGCCT 
CCTGTTGCTG TGTTGCCCCT GGGTACTGGA AATGATCTGG CTCGATGCCT AAGATGGGGA 
GGAGGTTATG AAGGACAGAA TCTGGCAAAG ATCCTCAAGG ATTTAGAGAT GAGTAAAGTG 
GTACATATGG ATCGATGGTC TGTGGAGGTG ATACCTCAAC AAACTGAAGA AAAAAGTGAC 
CCAGTCCCCT TTCAAATCAT CAATAACTAC TTCTCTATTG GCGTGGATGC CTCTATTGCT 
CATCGATTCC ACATCATGCG AGAGAAATAT CCGGAGAAGT TCAACAGCAG AATGAAGAAC 
AAGCTATGGT ACTTCGAATT TGCCACATCT GAATCCATCT TCTCAACATG CAAAAAGCTG 
GAGGAGTCTT TGACAGTTGA GATCTGTGGG AAACCGCTGG ATCTGAGCAA CCTGTCCCTA 
GAAGGCATCG CAGTGCTAAA CATCCCTAGC ATGCATGGTG GCTCCAACCT CTGGGGTGAT 
ACCAGGAGAC CCCATGGGGA TATCTATGGG ATCAACCAGG CCTTAGGTGC TACAGCTAAA 
GTCATCACCG ACCCTGATAT CCTGAAAACC TGTGTACCAG ACCTAAGTGA CAAGAGACTG 
GAAGTGGTTG GGCTGGAGGG TGCAATTGAG ATGGGCCAAA TCTATACCAA GCTCAAGAAT 
GCTGGACGTC GGCTGGCCAA GTGCTCTGAG ATCACCTTCC ACACCACAAA AACCCTTCCC 
ATGCAAATTG ACGGAGAACC CTGGATGCAG ACGCCCTGTA CAATCAAGAT CACCCACAAG 
AACCAGATGC CCATGCTCAT GGGCCCACCC CCCCGCTCCA CCAATTTCTT TGGCTTCTTG 
AGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001337969.1
CDS	1283..2428
Translation	MDDLNLSTSEALRIDPVPNTHPLLVFVNPKSGGKQGQRVLWKFQYILNPRQVFNLLKDGPEIGLRLFKDVPDSRILVCGGDGTVGWILETIDKANLPVLPPVAVLPLGTGNDLARCLRWGGGYEGQNLAKILKDLEMSKVVHMDRWSVEVIPQQTEEKSDPVPFQIINNYFSIGVDASIAHRFHIMREKYPEKFNSRMKNKLWYFEFATSESIFSTCKKLEESLTVEICGKPLDLSNLSLEGIAVLNIPSMHGGSNLWGDTRRPHGDIYGINQALGATAKVITDPDILKTCVPDLSDKRLEVVGLEGAIEMGQIYTKLKNAGRRLAKCSEITFHTTKTLPMQIDGEPWMQTPCTIKITHKNQMPMLMGPPPRSTNFFGFLS

Target ORF information:

RefSeq Version	NM_001351040.1
Organism	Homo sapiens(human)
Definition	Homo sapiens diacylglycerol kinase alpha (DGKA), transcript variant 12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001351040.1

ORF Insert Sequence:

ATGGATGATT TAAATTTGAG CACCTCTGAG GCTCTGCGGA TTGACCCTGT TCCTAACACC 
CACCCACTTC TCGTCTTTGT CAATCCTAAG AGTGGCGGGA AGCAGGGGCA AAGGGTGCTC 
TGGAAGTTCC AGTATATATT AAACCCTCGA CAGGTGTTCA ACCTCCTAAA GGATGGTCCT 
GAGATAGGGC TCCGATTATT CAAGGATGTT CCTGATAGCC GGATTTTGGT GTGTGGTGGA 
GACGGCACAG TAGGCTGGAT TCTAGAGACC ATTGACAAAG CTAACTTGCC AGTTTTGCCT 
CCTGTTGCTG TGTTGCCCCT GGGTACTGGA AATGATCTGG CTCGATGCCT AAGATGGGGA 
GGAGGTTATG AAGGACAGAA TCTGGCAAAG ATCCTCAAGG ATTTAGAGAT GAGTAAAGTG 
GTACATATGG ATCGATGGTC TGTGGAGGTG ATACCTCAAC AAACTGAAGA AAAAAGTGAC 
CCAGTCCCCT TTCAAATCAT CAATAACTAC TTCTCTATTG GCGTGGATGC CTCTATTGCT 
CATCGATTCC ACATCATGCG AGAGAAATAT CCGGAGAAGT TCAACAGCAG AATGAAGAAC 
AAGCTATGGT ACTTCGAATT TGCCACATCT GAATCCATCT TCTCAACATG CAAAAAGCTG 
GAGGAGTCTT TGACAGTTGA GATCTGTGGG AAACCGCTGG ATCTGAGCAA CCTGTCCCTA 
GAAGGCATCG CAGTGCTAAA CATCCCTAGC ATGCATGGTG GCTCCAACCT CTGGGGTGAT 
ACCAGGAGAC CCCATGGGGA TATCTATGGG ATCAACCAGG CCTTAGGTGC TACAGCTAAA 
GTCATCACCG ACCCTGATAT CCTGAAAACC TGTGTACCAG ACCTAAGTGA CAAGAGACTG 
GAAGTGGTTG GGCTGGAGGG TGCAATTGAG ATGGGCCAAA TCTATACCAA GCTCAAGAAT 
GCTGGACGTC GGCTGGCCAA GTGCTCTGAG ATCACCTTCC ACACCACAAA AACCCTTCCC 
ATGCAAATTG ACGGAGAACC CTGGATGCAG ACGCCCTGTA CAATCAAGAT CACCCACAAG 
AACCAGATGC CCATGCTCAT GGGCCCACCC CCCCGCTCCA CCAATTTCTT TGGCTTCTTG 
AGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DGKA ( XM_005268689.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_005268746.1 Homo sapiens diacylglycerol kinase alpha (DGKA), transcript variant X13, mRNA.

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CloneID	OHu46356
Clone ID Related Accession (Same CDS sequence)	XM_005268689.2 , XM_011537995.2 , XM_005268690.2
Accession Version	XM_005268689.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1872bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	diacylglycerol kinase alpha isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005268689.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCTTAA GAAACAGAAG AGGATGGGGG GAGAGGTTGA GTCCTCTAGG ACTAAATTTG 
AAAGAAGTGG ACAAAATTAT CCTACAGATG ATGCGAGTGG CTGAATACCT GGATTGGGAT 
GTGTCTGAGC TGAGGCCGAT TCTTCAGGAG ATGATGAAAG AGATTGACTA TGATGGCAGT 
GGCTCTGTCT CTCAAGCTGA GTGGGTCCGG GCTGGGGCCA CCACCGTGCC ACTGCTAGTG 
CTGCTGGGTC TGGAGATGAC TCTGAAGGAC GACGGACAGC ACATGTGGAG GCCCAAGAGG 
TTCCCCAGAC CAGTCTACTG CAATCTGTGC GAGTCAAGCA TTGGTCTTGG CAAACAGGGA 
CTGAGCTGTA ACCTCTGTAA GTACACTGTT CACGACCAGT GTGCCATGAA AGCCCTGCCT 
TGTGAAGTCA GCACCTATGC CAAGTCTCGG AAGGACATTG GTGTCCAATC ACATGTGTGG 
GTGCGAGGAG GCTGTGAGTC CGGGCGCTGC GACCGCTGTC AGAAAAAGAT CCGGATCTAC 
CACAGTCTGA CCGGGCTGCA TTGTGTATGG TGCCACCTAG AGATCCACGA TGACTGCCTG 
CAAGCGGTGG GCCATGAGTG TGACTGTGGG CTGCTCCGGG ATCACATCCT GCCTCCATCT 
TCCATCTATC CCAGTGTCCT GGCCTCTGGA CCGGATCGTA AAAATAGCAA AACAAGCCAG 
AAGACCATGG ATGATTTAAA TTTGAGCACC TCTGAGGCTC TGCGGATTGA CCCTGTTCCT 
AACACCCACC CACTTCTCGT CTTTGTCAAT CCTAAGAGTG GCGGGAAGCA GGGGCAAAGG 
GTGCTCTGGA AGTTCCAGTA TATATTAAAC CCTCGACAGG TGTTCAACCT CCTAAAGGAT 
GGTCCTGAGA TAGGGCTCCG ATTATTCAAG GATGTTCCTG ATAGCCGGAT TTTGGTGTGT 
GGTGGAGACG GCACAGTAGG CTGGATTCTA GAGACCATTG ACAAAGCTAA CTTGCCAGTT 
TTGCCTCCTG TTGCTGTGTT GCCCCTGGGT ACTGGAAATG ATCTGGCTCG ATGCCTAAGA 
TGGGGAGGAG GTTATGAAGG ACAGAATCTG GCAAAGATCC TCAAGGATTT AGAGATGAGT 
AAAGTGGTAC ATATGGATCG ATGGTCTGTG GAGGTGATAC CTCAACAAAC TGAAGAAAAA 
AGTGACCCAG TCCCCTTTCA AATCATCAAT AACTACTTCT CTATTGGCGT GGATGCCTCT 
ATTGCTCATC GATTCCACAT CATGCGAGAG AAATATCCGG AGAAGTTCAA CAGCAGAATG 
AAGAACAAGC TATGGTACTT CGAATTTGCC ACATCTGAAT CCATCTTCTC AACATGCAAA 
AAGCTGGAGG AGTCTTTGAC AGTTGAGATC TGTGGGAAAC CGCTGGATCT GAGCAACCTG 
TCCCTAGAAG GCATCGCAGT GCTAAACATC CCTAGCATGC ATGGTGGCTC CAACCTCTGG 
GGTGATACCA GGAGACCCCA TGGGGATATC TATGGGATCA ACCAGGCCTT AGGTGCTACA 
GCTAAAGTCA TCACCGACCC TGATATCCTG AAAACCTGTG TACCAGACCT AAGTGACAAG 
AGACTGGAAG TGGTTGGGCT GGAGGGTGCA ATTGAGATGG GCCAAATCTA TACCAAGCTC 
AAGAATGCTG GACGTCGGCT GGCCAAGTGC TCTGAGATCA CCTTCCACAC CACAAAAACC 
CTTCCCATGC AAATTGACGG AGAACCCTGG ATGCAGACGC CCTGTACAAT CAAGATCACC 
CACAAGAACC AGATGCCCAT GCTCATGGGC CCACCCCCCC GCTCCACCAA TTTCTTTGGC 
TTCTTGAGCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005268746.1
CDS	478..2349
Translation	MVLRNRRGWGERLSPLGLNLKEVDKIILQMMRVAEYLDWDVSELRPILQEMMKEIDYDGSGSVSQAEWVRAGATTVPLLVLLGLEMTLKDDGQHMWRPKRFPRPVYCNLCESSIGLGKQGLSCNLCKYTVHDQCAMKALPCEVSTYAKSRKDIGVQSHVWVRGGCESGRCDRCQKKIRIYHSLTGLHCVWCHLEIHDDCLQAVGHECDCGLLRDHILPPSSIYPSVLASGPDRKNSKTSQKTMDDLNLSTSEALRIDPVPNTHPLLVFVNPKSGGKQGQRVLWKFQYILNPRQVFNLLKDGPEIGLRLFKDVPDSRILVCGGDGTVGWILETIDKANLPVLPPVAVLPLGTGNDLARCLRWGGGYEGQNLAKILKDLEMSKVVHMDRWSVEVIPQQTEEKSDPVPFQIINNYFSIGVDASIAHRFHIMREKYPEKFNSRMKNKLWYFEFATSESIFSTCKKLEESLTVEICGKPLDLSNLSLEGIAVLNIPSMHGGSNLWGDTRRPHGDIYGINQALGATAKVITDPDILKTCVPDLSDKRLEVVGLEGAIEMGQIYTKLKNAGRRLAKCSEITFHTTKTLPMQIDGEPWMQTPCTIKITHKNQMPMLMGPPPRSTNFFGFLS

Target ORF information:

RefSeq Version	XM_005268689.2
Organism	Homo sapiens(human)
Definition	Homo sapiens diacylglycerol kinase alpha (DGKA), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005268689.2

ORF Insert Sequence:

ATGGTCTTAA GAAACAGAAG AGGATGGGGG GAGAGGTTGA GTCCTCTAGG ACTAAATTTG 
AAAGAAGTGG ACAAAATTAT CCTACAGATG ATGCGAGTGG CTGAATACCT GGATTGGGAT 
GTGTCTGAGC TGAGGCCGAT TCTTCAGGAG ATGATGAAAG AGATTGACTA TGATGGCAGT 
GGCTCTGTCT CTCAAGCTGA GTGGGTCCGG GCTGGGGCCA CCACCGTGCC ACTGCTAGTG 
CTGCTGGGTC TGGAGATGAC TCTGAAGGAC GACGGACAGC ACATGTGGAG GCCCAAGAGG 
TTCCCCAGAC CAGTCTACTG CAATCTGTGC GAGTCAAGCA TTGGTCTTGG CAAACAGGGA 
CTGAGCTGTA ACCTCTGTAA GTACACTGTT CACGACCAGT GTGCCATGAA AGCCCTGCCT 
TGTGAAGTCA GCACCTATGC CAAGTCTCGG AAGGACATTG GTGTCCAATC ACATGTGTGG 
GTGCGAGGAG GCTGTGAGTC CGGGCGCTGC GACCGCTGTC AGAAAAAGAT CCGGATCTAC 
CACAGTCTGA CCGGGCTGCA TTGTGTATGG TGCCACCTAG AGATCCACGA TGACTGCCTG 
CAAGCGGTGG GCCATGAGTG TGACTGTGGG CTGCTCCGGG ATCACATCCT GCCTCCATCT 
TCCATCTATC CCAGTGTCCT GGCCTCTGGA CCGGATCGTA AAAATAGCAA AACAAGCCAG 
AAGACCATGG ATGATTTAAA TTTGAGCACC TCTGAGGCTC TGCGGATTGA CCCTGTTCCT 
AACACCCACC CACTTCTCGT CTTTGTCAAT CCTAAGAGTG GCGGGAAGCA GGGGCAAAGG 
GTGCTCTGGA AGTTCCAGTA TATATTAAAC CCTCGACAGG TGTTCAACCT CCTAAAGGAT 
GGTCCTGAGA TAGGGCTCCG ATTATTCAAG GATGTTCCTG ATAGCCGGAT TTTGGTGTGT 
GGTGGAGACG GCACAGTAGG CTGGATTCTA GAGACCATTG ACAAAGCTAA CTTGCCAGTT 
TTGCCTCCTG TTGCTGTGTT GCCCCTGGGT ACTGGAAATG ATCTGGCTCG ATGCCTAAGA 
TGGGGAGGAG GTTATGAAGG ACAGAATCTG GCAAAGATCC TCAAGGATTT AGAGATGAGT 
AAAGTGGTAC ATATGGATCG ATGGTCTGTG GAGGTGATAC CTCAACAAAC TGAAGAAAAA 
AGTGACCCAG TCCCCTTTCA AATCATCAAT AACTACTTCT CTATTGGCGT GGATGCCTCT 
ATTGCTCATC GATTCCACAT CATGCGAGAG AAATATCCGG AGAAGTTCAA CAGCAGAATG 
AAGAACAAGC TATGGTACTT CGAATTTGCC ACATCTGAAT CCATCTTCTC AACATGCAAA 
AAGCTGGAGG AGTCTTTGAC AGTTGAGATC TGTGGGAAAC CGCTGGATCT GAGCAACCTG 
TCCCTAGAAG GCATCGCAGT GCTAAACATC CCTAGCATGC ATGGTGGCTC CAACCTCTGG 
GGTGATACCA GGAGACCCCA TGGGGATATC TATGGGATCA ACCAGGCCTT AGGTGCTACA 
GCTAAAGTCA TCACCGACCC TGATATCCTG AAAACCTGTG TACCAGACCT AAGTGACAAG 
AGACTGGAAG TGGTTGGGCT GGAGGGTGCA ATTGAGATGG GCCAAATCTA TACCAAGCTC 
AAGAATGCTG GACGTCGGCT GGCCAAGTGC TCTGAGATCA CCTTCCACAC CACAAAAACC 
CTTCCCATGC AAATTGACGG AGAACCCTGG ATGCAGACGC CCTGTACAAT CAAGATCACC 
CACAAGAACC AGATGCCCAT GCTCATGGGC CCACCCCCCC GCTCCACCAA TTTCTTTGGC 
TTCTTGAGCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DGKA ( XM_011537995.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011536297.1 Homo sapiens diacylglycerol kinase alpha (DGKA), transcript variant X14, mRNA.

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CloneID	OHu46356
Clone ID Related Accession (Same CDS sequence)	XM_005268689.2 , XM_011537995.2 , XM_005268690.2
Accession Version	XM_011537995.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1872bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	diacylglycerol kinase alpha isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011537995.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCTTAA GAAACAGAAG AGGATGGGGG GAGAGGTTGA GTCCTCTAGG ACTAAATTTG 
AAAGAAGTGG ACAAAATTAT CCTACAGATG ATGCGAGTGG CTGAATACCT GGATTGGGAT 
GTGTCTGAGC TGAGGCCGAT TCTTCAGGAG ATGATGAAAG AGATTGACTA TGATGGCAGT 
GGCTCTGTCT CTCAAGCTGA GTGGGTCCGG GCTGGGGCCA CCACCGTGCC ACTGCTAGTG 
CTGCTGGGTC TGGAGATGAC TCTGAAGGAC GACGGACAGC ACATGTGGAG GCCCAAGAGG 
TTCCCCAGAC CAGTCTACTG CAATCTGTGC GAGTCAAGCA TTGGTCTTGG CAAACAGGGA 
CTGAGCTGTA ACCTCTGTAA GTACACTGTT CACGACCAGT GTGCCATGAA AGCCCTGCCT 
TGTGAAGTCA GCACCTATGC CAAGTCTCGG AAGGACATTG GTGTCCAATC ACATGTGTGG 
GTGCGAGGAG GCTGTGAGTC CGGGCGCTGC GACCGCTGTC AGAAAAAGAT CCGGATCTAC 
CACAGTCTGA CCGGGCTGCA TTGTGTATGG TGCCACCTAG AGATCCACGA TGACTGCCTG 
CAAGCGGTGG GCCATGAGTG TGACTGTGGG CTGCTCCGGG ATCACATCCT GCCTCCATCT 
TCCATCTATC CCAGTGTCCT GGCCTCTGGA CCGGATCGTA AAAATAGCAA AACAAGCCAG 
AAGACCATGG ATGATTTAAA TTTGAGCACC TCTGAGGCTC TGCGGATTGA CCCTGTTCCT 
AACACCCACC CACTTCTCGT CTTTGTCAAT CCTAAGAGTG GCGGGAAGCA GGGGCAAAGG 
GTGCTCTGGA AGTTCCAGTA TATATTAAAC CCTCGACAGG TGTTCAACCT CCTAAAGGAT 
GGTCCTGAGA TAGGGCTCCG ATTATTCAAG GATGTTCCTG ATAGCCGGAT TTTGGTGTGT 
GGTGGAGACG GCACAGTAGG CTGGATTCTA GAGACCATTG ACAAAGCTAA CTTGCCAGTT 
TTGCCTCCTG TTGCTGTGTT GCCCCTGGGT ACTGGAAATG ATCTGGCTCG ATGCCTAAGA 
TGGGGAGGAG GTTATGAAGG ACAGAATCTG GCAAAGATCC TCAAGGATTT AGAGATGAGT 
AAAGTGGTAC ATATGGATCG ATGGTCTGTG GAGGTGATAC CTCAACAAAC TGAAGAAAAA 
AGTGACCCAG TCCCCTTTCA AATCATCAAT AACTACTTCT CTATTGGCGT GGATGCCTCT 
ATTGCTCATC GATTCCACAT CATGCGAGAG AAATATCCGG AGAAGTTCAA CAGCAGAATG 
AAGAACAAGC TATGGTACTT CGAATTTGCC ACATCTGAAT CCATCTTCTC AACATGCAAA 
AAGCTGGAGG AGTCTTTGAC AGTTGAGATC TGTGGGAAAC CGCTGGATCT GAGCAACCTG 
TCCCTAGAAG GCATCGCAGT GCTAAACATC CCTAGCATGC ATGGTGGCTC CAACCTCTGG 
GGTGATACCA GGAGACCCCA TGGGGATATC TATGGGATCA ACCAGGCCTT AGGTGCTACA 
GCTAAAGTCA TCACCGACCC TGATATCCTG AAAACCTGTG TACCAGACCT AAGTGACAAG 
AGACTGGAAG TGGTTGGGCT GGAGGGTGCA ATTGAGATGG GCCAAATCTA TACCAAGCTC 
AAGAATGCTG GACGTCGGCT GGCCAAGTGC TCTGAGATCA CCTTCCACAC CACAAAAACC 
CTTCCCATGC AAATTGACGG AGAACCCTGG ATGCAGACGC CCTGTACAAT CAAGATCACC 
CACAAGAACC AGATGCCCAT GCTCATGGGC CCACCCCCCC GCTCCACCAA TTTCTTTGGC 
TTCTTGAGCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011536297.1
CDS	473..2344
Translation	MVLRNRRGWGERLSPLGLNLKEVDKIILQMMRVAEYLDWDVSELRPILQEMMKEIDYDGSGSVSQAEWVRAGATTVPLLVLLGLEMTLKDDGQHMWRPKRFPRPVYCNLCESSIGLGKQGLSCNLCKYTVHDQCAMKALPCEVSTYAKSRKDIGVQSHVWVRGGCESGRCDRCQKKIRIYHSLTGLHCVWCHLEIHDDCLQAVGHECDCGLLRDHILPPSSIYPSVLASGPDRKNSKTSQKTMDDLNLSTSEALRIDPVPNTHPLLVFVNPKSGGKQGQRVLWKFQYILNPRQVFNLLKDGPEIGLRLFKDVPDSRILVCGGDGTVGWILETIDKANLPVLPPVAVLPLGTGNDLARCLRWGGGYEGQNLAKILKDLEMSKVVHMDRWSVEVIPQQTEEKSDPVPFQIINNYFSIGVDASIAHRFHIMREKYPEKFNSRMKNKLWYFEFATSESIFSTCKKLEESLTVEICGKPLDLSNLSLEGIAVLNIPSMHGGSNLWGDTRRPHGDIYGINQALGATAKVITDPDILKTCVPDLSDKRLEVVGLEGAIEMGQIYTKLKNAGRRLAKCSEITFHTTKTLPMQIDGEPWMQTPCTIKITHKNQMPMLMGPPPRSTNFFGFLS

Target ORF information:

RefSeq Version	XM_011537995.2
Organism	Homo sapiens(human)
Definition	Homo sapiens diacylglycerol kinase alpha (DGKA), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011537995.2

ORF Insert Sequence:

ATGGTCTTAA GAAACAGAAG AGGATGGGGG GAGAGGTTGA GTCCTCTAGG ACTAAATTTG 
AAAGAAGTGG ACAAAATTAT CCTACAGATG ATGCGAGTGG CTGAATACCT GGATTGGGAT 
GTGTCTGAGC TGAGGCCGAT TCTTCAGGAG ATGATGAAAG AGATTGACTA TGATGGCAGT 
GGCTCTGTCT CTCAAGCTGA GTGGGTCCGG GCTGGGGCCA CCACCGTGCC ACTGCTAGTG 
CTGCTGGGTC TGGAGATGAC TCTGAAGGAC GACGGACAGC ACATGTGGAG GCCCAAGAGG 
TTCCCCAGAC CAGTCTACTG CAATCTGTGC GAGTCAAGCA TTGGTCTTGG CAAACAGGGA 
CTGAGCTGTA ACCTCTGTAA GTACACTGTT CACGACCAGT GTGCCATGAA AGCCCTGCCT 
TGTGAAGTCA GCACCTATGC CAAGTCTCGG AAGGACATTG GTGTCCAATC ACATGTGTGG 
GTGCGAGGAG GCTGTGAGTC CGGGCGCTGC GACCGCTGTC AGAAAAAGAT CCGGATCTAC 
CACAGTCTGA CCGGGCTGCA TTGTGTATGG TGCCACCTAG AGATCCACGA TGACTGCCTG 
CAAGCGGTGG GCCATGAGTG TGACTGTGGG CTGCTCCGGG ATCACATCCT GCCTCCATCT 
TCCATCTATC CCAGTGTCCT GGCCTCTGGA CCGGATCGTA AAAATAGCAA AACAAGCCAG 
AAGACCATGG ATGATTTAAA TTTGAGCACC TCTGAGGCTC TGCGGATTGA CCCTGTTCCT 
AACACCCACC CACTTCTCGT CTTTGTCAAT CCTAAGAGTG GCGGGAAGCA GGGGCAAAGG 
GTGCTCTGGA AGTTCCAGTA TATATTAAAC CCTCGACAGG TGTTCAACCT CCTAAAGGAT 
GGTCCTGAGA TAGGGCTCCG ATTATTCAAG GATGTTCCTG ATAGCCGGAT TTTGGTGTGT 
GGTGGAGACG GCACAGTAGG CTGGATTCTA GAGACCATTG ACAAAGCTAA CTTGCCAGTT 
TTGCCTCCTG TTGCTGTGTT GCCCCTGGGT ACTGGAAATG ATCTGGCTCG ATGCCTAAGA 
TGGGGAGGAG GTTATGAAGG ACAGAATCTG GCAAAGATCC TCAAGGATTT AGAGATGAGT 
AAAGTGGTAC ATATGGATCG ATGGTCTGTG GAGGTGATAC CTCAACAAAC TGAAGAAAAA 
AGTGACCCAG TCCCCTTTCA AATCATCAAT AACTACTTCT CTATTGGCGT GGATGCCTCT 
ATTGCTCATC GATTCCACAT CATGCGAGAG AAATATCCGG AGAAGTTCAA CAGCAGAATG 
AAGAACAAGC TATGGTACTT CGAATTTGCC ACATCTGAAT CCATCTTCTC AACATGCAAA 
AAGCTGGAGG AGTCTTTGAC AGTTGAGATC TGTGGGAAAC CGCTGGATCT GAGCAACCTG 
TCCCTAGAAG GCATCGCAGT GCTAAACATC CCTAGCATGC ATGGTGGCTC CAACCTCTGG 
GGTGATACCA GGAGACCCCA TGGGGATATC TATGGGATCA ACCAGGCCTT AGGTGCTACA 
GCTAAAGTCA TCACCGACCC TGATATCCTG AAAACCTGTG TACCAGACCT AAGTGACAAG 
AGACTGGAAG TGGTTGGGCT GGAGGGTGCA ATTGAGATGG GCCAAATCTA TACCAAGCTC 
AAGAATGCTG GACGTCGGCT GGCCAAGTGC TCTGAGATCA CCTTCCACAC CACAAAAACC 
CTTCCCATGC AAATTGACGG AGAACCCTGG ATGCAGACGC CCTGTACAAT CAAGATCACC 
CACAAGAACC AGATGCCCAT GCTCATGGGC CCACCCCCCC GCTCCACCAA TTTCTTTGGC 
TTCTTGAGCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DGKA ( XM_005268690.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_005268747.1 Homo sapiens diacylglycerol kinase alpha (DGKA), transcript variant X17, mRNA.

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CloneID	OHu46356
Clone ID Related Accession (Same CDS sequence)	XM_005268689.2 , XM_011537995.2 , XM_005268690.2
Accession Version	XM_005268690.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1872bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	diacylglycerol kinase alpha isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005268690.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCTTAA GAAACAGAAG AGGATGGGGG GAGAGGTTGA GTCCTCTAGG ACTAAATTTG 
AAAGAAGTGG ACAAAATTAT CCTACAGATG ATGCGAGTGG CTGAATACCT GGATTGGGAT 
GTGTCTGAGC TGAGGCCGAT TCTTCAGGAG ATGATGAAAG AGATTGACTA TGATGGCAGT 
GGCTCTGTCT CTCAAGCTGA GTGGGTCCGG GCTGGGGCCA CCACCGTGCC ACTGCTAGTG 
CTGCTGGGTC TGGAGATGAC TCTGAAGGAC GACGGACAGC ACATGTGGAG GCCCAAGAGG 
TTCCCCAGAC CAGTCTACTG CAATCTGTGC GAGTCAAGCA TTGGTCTTGG CAAACAGGGA 
CTGAGCTGTA ACCTCTGTAA GTACACTGTT CACGACCAGT GTGCCATGAA AGCCCTGCCT 
TGTGAAGTCA GCACCTATGC CAAGTCTCGG AAGGACATTG GTGTCCAATC ACATGTGTGG 
GTGCGAGGAG GCTGTGAGTC CGGGCGCTGC GACCGCTGTC AGAAAAAGAT CCGGATCTAC 
CACAGTCTGA CCGGGCTGCA TTGTGTATGG TGCCACCTAG AGATCCACGA TGACTGCCTG 
CAAGCGGTGG GCCATGAGTG TGACTGTGGG CTGCTCCGGG ATCACATCCT GCCTCCATCT 
TCCATCTATC CCAGTGTCCT GGCCTCTGGA CCGGATCGTA AAAATAGCAA AACAAGCCAG 
AAGACCATGG ATGATTTAAA TTTGAGCACC TCTGAGGCTC TGCGGATTGA CCCTGTTCCT 
AACACCCACC CACTTCTCGT CTTTGTCAAT CCTAAGAGTG GCGGGAAGCA GGGGCAAAGG 
GTGCTCTGGA AGTTCCAGTA TATATTAAAC CCTCGACAGG TGTTCAACCT CCTAAAGGAT 
GGTCCTGAGA TAGGGCTCCG ATTATTCAAG GATGTTCCTG ATAGCCGGAT TTTGGTGTGT 
GGTGGAGACG GCACAGTAGG CTGGATTCTA GAGACCATTG ACAAAGCTAA CTTGCCAGTT 
TTGCCTCCTG TTGCTGTGTT GCCCCTGGGT ACTGGAAATG ATCTGGCTCG ATGCCTAAGA 
TGGGGAGGAG GTTATGAAGG ACAGAATCTG GCAAAGATCC TCAAGGATTT AGAGATGAGT 
AAAGTGGTAC ATATGGATCG ATGGTCTGTG GAGGTGATAC CTCAACAAAC TGAAGAAAAA 
AGTGACCCAG TCCCCTTTCA AATCATCAAT AACTACTTCT CTATTGGCGT GGATGCCTCT 
ATTGCTCATC GATTCCACAT CATGCGAGAG AAATATCCGG AGAAGTTCAA CAGCAGAATG 
AAGAACAAGC TATGGTACTT CGAATTTGCC ACATCTGAAT CCATCTTCTC AACATGCAAA 
AAGCTGGAGG AGTCTTTGAC AGTTGAGATC TGTGGGAAAC CGCTGGATCT GAGCAACCTG 
TCCCTAGAAG GCATCGCAGT GCTAAACATC CCTAGCATGC ATGGTGGCTC CAACCTCTGG 
GGTGATACCA GGAGACCCCA TGGGGATATC TATGGGATCA ACCAGGCCTT AGGTGCTACA 
GCTAAAGTCA TCACCGACCC TGATATCCTG AAAACCTGTG TACCAGACCT AAGTGACAAG 
AGACTGGAAG TGGTTGGGCT GGAGGGTGCA ATTGAGATGG GCCAAATCTA TACCAAGCTC 
AAGAATGCTG GACGTCGGCT GGCCAAGTGC TCTGAGATCA CCTTCCACAC CACAAAAACC 
CTTCCCATGC AAATTGACGG AGAACCCTGG ATGCAGACGC CCTGTACAAT CAAGATCACC 
CACAAGAACC AGATGCCCAT GCTCATGGGC CCACCCCCCC GCTCCACCAA TTTCTTTGGC 
TTCTTGAGCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005268747.1
CDS	298..2169
Translation	MVLRNRRGWGERLSPLGLNLKEVDKIILQMMRVAEYLDWDVSELRPILQEMMKEIDYDGSGSVSQAEWVRAGATTVPLLVLLGLEMTLKDDGQHMWRPKRFPRPVYCNLCESSIGLGKQGLSCNLCKYTVHDQCAMKALPCEVSTYAKSRKDIGVQSHVWVRGGCESGRCDRCQKKIRIYHSLTGLHCVWCHLEIHDDCLQAVGHECDCGLLRDHILPPSSIYPSVLASGPDRKNSKTSQKTMDDLNLSTSEALRIDPVPNTHPLLVFVNPKSGGKQGQRVLWKFQYILNPRQVFNLLKDGPEIGLRLFKDVPDSRILVCGGDGTVGWILETIDKANLPVLPPVAVLPLGTGNDLARCLRWGGGYEGQNLAKILKDLEMSKVVHMDRWSVEVIPQQTEEKSDPVPFQIINNYFSIGVDASIAHRFHIMREKYPEKFNSRMKNKLWYFEFATSESIFSTCKKLEESLTVEICGKPLDLSNLSLEGIAVLNIPSMHGGSNLWGDTRRPHGDIYGINQALGATAKVITDPDILKTCVPDLSDKRLEVVGLEGAIEMGQIYTKLKNAGRRLAKCSEITFHTTKTLPMQIDGEPWMQTPCTIKITHKNQMPMLMGPPPRSTNFFGFLS

Target ORF information:

RefSeq Version	XM_005268690.2
Organism	Homo sapiens(human)
Definition	Homo sapiens diacylglycerol kinase alpha (DGKA), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005268690.2

ORF Insert Sequence:

ATGGTCTTAA GAAACAGAAG AGGATGGGGG GAGAGGTTGA GTCCTCTAGG ACTAAATTTG 
AAAGAAGTGG ACAAAATTAT CCTACAGATG ATGCGAGTGG CTGAATACCT GGATTGGGAT 
GTGTCTGAGC TGAGGCCGAT TCTTCAGGAG ATGATGAAAG AGATTGACTA TGATGGCAGT 
GGCTCTGTCT CTCAAGCTGA GTGGGTCCGG GCTGGGGCCA CCACCGTGCC ACTGCTAGTG 
CTGCTGGGTC TGGAGATGAC TCTGAAGGAC GACGGACAGC ACATGTGGAG GCCCAAGAGG 
TTCCCCAGAC CAGTCTACTG CAATCTGTGC GAGTCAAGCA TTGGTCTTGG CAAACAGGGA 
CTGAGCTGTA ACCTCTGTAA GTACACTGTT CACGACCAGT GTGCCATGAA AGCCCTGCCT 
TGTGAAGTCA GCACCTATGC CAAGTCTCGG AAGGACATTG GTGTCCAATC ACATGTGTGG 
GTGCGAGGAG GCTGTGAGTC CGGGCGCTGC GACCGCTGTC AGAAAAAGAT CCGGATCTAC 
CACAGTCTGA CCGGGCTGCA TTGTGTATGG TGCCACCTAG AGATCCACGA TGACTGCCTG 
CAAGCGGTGG GCCATGAGTG TGACTGTGGG CTGCTCCGGG ATCACATCCT GCCTCCATCT 
TCCATCTATC CCAGTGTCCT GGCCTCTGGA CCGGATCGTA AAAATAGCAA AACAAGCCAG 
AAGACCATGG ATGATTTAAA TTTGAGCACC TCTGAGGCTC TGCGGATTGA CCCTGTTCCT 
AACACCCACC CACTTCTCGT CTTTGTCAAT CCTAAGAGTG GCGGGAAGCA GGGGCAAAGG 
GTGCTCTGGA AGTTCCAGTA TATATTAAAC CCTCGACAGG TGTTCAACCT CCTAAAGGAT 
GGTCCTGAGA TAGGGCTCCG ATTATTCAAG GATGTTCCTG ATAGCCGGAT TTTGGTGTGT 
GGTGGAGACG GCACAGTAGG CTGGATTCTA GAGACCATTG ACAAAGCTAA CTTGCCAGTT 
TTGCCTCCTG TTGCTGTGTT GCCCCTGGGT ACTGGAAATG ATCTGGCTCG ATGCCTAAGA 
TGGGGAGGAG GTTATGAAGG ACAGAATCTG GCAAAGATCC TCAAGGATTT AGAGATGAGT 
AAAGTGGTAC ATATGGATCG ATGGTCTGTG GAGGTGATAC CTCAACAAAC TGAAGAAAAA 
AGTGACCCAG TCCCCTTTCA AATCATCAAT AACTACTTCT CTATTGGCGT GGATGCCTCT 
ATTGCTCATC GATTCCACAT CATGCGAGAG AAATATCCGG AGAAGTTCAA CAGCAGAATG 
AAGAACAAGC TATGGTACTT CGAATTTGCC ACATCTGAAT CCATCTTCTC AACATGCAAA 
AAGCTGGAGG AGTCTTTGAC AGTTGAGATC TGTGGGAAAC CGCTGGATCT GAGCAACCTG 
TCCCTAGAAG GCATCGCAGT GCTAAACATC CCTAGCATGC ATGGTGGCTC CAACCTCTGG 
GGTGATACCA GGAGACCCCA TGGGGATATC TATGGGATCA ACCAGGCCTT AGGTGCTACA 
GCTAAAGTCA TCACCGACCC TGATATCCTG AAAACCTGTG TACCAGACCT AAGTGACAAG 
AGACTGGAAG TGGTTGGGCT GGAGGGTGCA ATTGAGATGG GCCAAATCTA TACCAAGCTC 
AAGAATGCTG GACGTCGGCT GGCCAAGTGC TCTGAGATCA CCTTCCACAC CACAAAAACC 
CTTCCCATGC AAATTGACGG AGAACCCTGG ATGCAGACGC CCTGTACAAT CAAGATCACC 
CACAAGAACC AGATGCCCAT GCTCATGGGC CCACCCCCCC GCTCCACCAA TTTCTTTGGC 
TTCTTGAGCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DGKA ( NM_001351036.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001337965.1 Homo sapiens diacylglycerol kinase alpha (DGKA), transcript variant 8, mRNA.

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CloneID	OHu102467
Clone ID Related Accession (Same CDS sequence)	NM_001351036.1 , NM_001351037.1
Accession Version	NM_001351036.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1785bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-30
Organism	Homo sapiens(human)
Product	diacylglycerol kinase alpha isoform c
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC025162.48. Transcript Variant: This variant (8), as well as variant 9, encodes isoform c. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.249828.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGATGCGAG TGGCTGAATA CCTGGATTGG GATGTGTCTG AGCTGAGGCC GATTCTTCAG 
GAGATGATGA AAGAGATTGA CTATGATGGC AGTGGCTCTG TCTCTCAAGC TGAGTGGGTC 
CGGGCTGGGG CCACCACCGT GCCACTGCTA GTGCTGCTGG GTCTGGAGAT GACTCTGAAG 
GACGACGGAC AGCACATGTG GAGGCCCAAG AGGTTCCCCA GACCAGTCTA CTGCAATCTG 
TGCGAGTCAA GCATTGGTCT TGGCAAACAG GGACTGAGCT GTAACCTCTG TAAGTACACT 
GTTCACGACC AGTGTGCCAT GAAAGCCCTG CCTTGTGAAG TCAGCACCTA TGCCAAGTCT 
CGGAAGGACA TTGGTGTCCA ATCACATGTG TGGGTGCGAG GAGGCTGTGA GTCCGGGCGC 
TGCGACCGCT GTCAGAAAAA GATCCGGATC TACCACAGTC TGACCGGGCT GCATTGTGTA 
TGGTGCCACC TAGAGATCCA CGATGACTGC CTGCAAGCGG TGGGCCATGA GTGTGACTGT 
GGGCTGCTCC GGGATCACAT CCTGCCTCCA TCTTCCATCT ATCCCAGTGT CCTGGCCTCT 
GGACCGGATC GTAAAAATAG CAAAACAAGC CAGAAGACCA TGGATGATTT AAATTTGAGC 
ACCTCTGAGG CTCTGCGGAT TGACCCTGTT CCTAACACCC ACCCACTTCT CGTCTTTGTC 
AATCCTAAGA GTGGCGGGAA GCAGGGGCAA AGGGTGCTCT GGAAGTTCCA GTATATATTA 
AACCCTCGAC AGGTGTTCAA CCTCCTAAAG GATGGTCCTG AGATAGGGCT CCGATTATTC 
AAGGATGTTC CTGATAGCCG GATTTTGGTG TGTGGTGGAG ACGGCACAGT AGGCTGGATT 
CTAGAGACCA TTGACAAAGC TAACTTGCCA GTTTTGCCTC CTGTTGCTGT GTTGCCCCTG 
GGTACTGGAA ATGATCTGGC TCGATGCCTA AGATGGGGAG GAGGTTATGA AGGACAGAAT 
CTGGCAAAGA TCCTCAAGGA TTTAGAGATG AGTAAAGTGG TACATATGGA TCGATGGTCT 
GTGGAGGTGA TACCTCAACA AACTGAAGAA AAAAGTGACC CAGTCCCCTT TCAAATCATC 
AATAACTACT TCTCTATTGG CGTGGATGCC TCTATTGCTC ATCGATTCCA CATCATGCGA 
GAGAAATATC CGGAGAAGTT CAACAGCAGA ATGAAGAACA AGCTATGGTA CTTCGAATTT 
GCCACATCTG AATCCATCTT CTCAACATGC AAAAAGCTGG AGGAGTCTTT GACAGTTGAG 
ATCTGTGGGA AACCGCTGGA TCTGAGCAAC CTGTCCCTAG AAGGCATCGC AGTGCTAAAC 
ATCCCTAGCA TGCATGGTGG CTCCAACCTC TGGGGTGATA CCAGGAGACC CCATGGGGAT 
ATCTATGGGA TCAACCAGGC CTTAGGTGCT ACAGCTAAAG TCATCACCGA CCCTGATATC 
CTGAAAACCT GTGTACCAGA CCTAAGTGAC AAGAGACTGG AAGTGGTTGG GCTGGAGGGT 
GCAATTGAGA TGGGCCAAAT CTATACCAAG CTCAAGAATG CTGGACGTCG GCTGGCCAAG 
TGCTCTGAGA TCACCTTCCA CACCACAAAA ACCCTTCCCA TGCAAATTGA CGGAGAACCC 
TGGATGCAGA CGCCCTGTAC AATCAAGATC ACCCACAAGA ACCAGATGCC CATGCTCATG 
GGCCCACCCC CCCGCTCCAC CAATTTCTTT GGCTTCTTGA GCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001337965.1
CDS	615..2399
Translation	MMRVAEYLDWDVSELRPILQEMMKEIDYDGSGSVSQAEWVRAGATTVPLLVLLGLEMTLKDDGQHMWRPKRFPRPVYCNLCESSIGLGKQGLSCNLCKYTVHDQCAMKALPCEVSTYAKSRKDIGVQSHVWVRGGCESGRCDRCQKKIRIYHSLTGLHCVWCHLEIHDDCLQAVGHECDCGLLRDHILPPSSIYPSVLASGPDRKNSKTSQKTMDDLNLSTSEALRIDPVPNTHPLLVFVNPKSGGKQGQRVLWKFQYILNPRQVFNLLKDGPEIGLRLFKDVPDSRILVCGGDGTVGWILETIDKANLPVLPPVAVLPLGTGNDLARCLRWGGGYEGQNLAKILKDLEMSKVVHMDRWSVEVIPQQTEEKSDPVPFQIINNYFSIGVDASIAHRFHIMREKYPEKFNSRMKNKLWYFEFATSESIFSTCKKLEESLTVEICGKPLDLSNLSLEGIAVLNIPSMHGGSNLWGDTRRPHGDIYGINQALGATAKVITDPDILKTCVPDLSDKRLEVVGLEGAIEMGQIYTKLKNAGRRLAKCSEITFHTTKTLPMQIDGEPWMQTPCTIKITHKNQMPMLMGPPPRSTNFFGFLS

Target ORF information:

RefSeq Version	NM_001351036.1
Organism	Homo sapiens(human)
Definition	Homo sapiens diacylglycerol kinase alpha (DGKA), transcript variant 8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001351036.1

ORF Insert Sequence:

ATGATGCGAG TGGCTGAATA CCTGGATTGG GATGTGTCTG AGCTGAGGCC GATTCTTCAG 
GAGATGATGA AAGAGATTGA CTATGATGGC AGTGGCTCTG TCTCTCAAGC TGAGTGGGTC 
CGGGCTGGGG CCACCACCGT GCCACTGCTA GTGCTGCTGG GTCTGGAGAT GACTCTGAAG 
GACGACGGAC AGCACATGTG GAGGCCCAAG AGGTTCCCCA GACCAGTCTA CTGCAATCTG 
TGCGAGTCAA GCATTGGTCT TGGCAAACAG GGACTGAGCT GTAACCTCTG TAAGTACACT 
GTTCACGACC AGTGTGCCAT GAAAGCCCTG CCTTGTGAAG TCAGCACCTA TGCCAAGTCT 
CGGAAGGACA TTGGTGTCCA ATCACATGTG TGGGTGCGAG GAGGCTGTGA GTCCGGGCGC 
TGCGACCGCT GTCAGAAAAA GATCCGGATC TACCACAGTC TGACCGGGCT GCATTGTGTA 
TGGTGCCACC TAGAGATCCA CGATGACTGC CTGCAAGCGG TGGGCCATGA GTGTGACTGT 
GGGCTGCTCC GGGATCACAT CCTGCCTCCA TCTTCCATCT ATCCCAGTGT CCTGGCCTCT 
GGACCGGATC GTAAAAATAG CAAAACAAGC CAGAAGACCA TGGATGATTT AAATTTGAGC 
ACCTCTGAGG CTCTGCGGAT TGACCCTGTT CCTAACACCC ACCCACTTCT CGTCTTTGTC 
AATCCTAAGA GTGGCGGGAA GCAGGGGCAA AGGGTGCTCT GGAAGTTCCA GTATATATTA 
AACCCTCGAC AGGTGTTCAA CCTCCTAAAG GATGGTCCTG AGATAGGGCT CCGATTATTC 
AAGGATGTTC CTGATAGCCG GATTTTGGTG TGTGGTGGAG ACGGCACAGT AGGCTGGATT 
CTAGAGACCA TTGACAAAGC TAACTTGCCA GTTTTGCCTC CTGTTGCTGT GTTGCCCCTG 
GGTACTGGAA ATGATCTGGC TCGATGCCTA AGATGGGGAG GAGGTTATGA AGGACAGAAT 
CTGGCAAAGA TCCTCAAGGA TTTAGAGATG AGTAAAGTGG TACATATGGA TCGATGGTCT 
GTGGAGGTGA TACCTCAACA AACTGAAGAA AAAAGTGACC CAGTCCCCTT TCAAATCATC 
AATAACTACT TCTCTATTGG CGTGGATGCC TCTATTGCTC ATCGATTCCA CATCATGCGA 
GAGAAATATC CGGAGAAGTT CAACAGCAGA ATGAAGAACA AGCTATGGTA CTTCGAATTT 
GCCACATCTG AATCCATCTT CTCAACATGC AAAAAGCTGG AGGAGTCTTT GACAGTTGAG 
ATCTGTGGGA AACCGCTGGA TCTGAGCAAC CTGTCCCTAG AAGGCATCGC AGTGCTAAAC 
ATCCCTAGCA TGCATGGTGG CTCCAACCTC TGGGGTGATA CCAGGAGACC CCATGGGGAT 
ATCTATGGGA TCAACCAGGC CTTAGGTGCT ACAGCTAAAG TCATCACCGA CCCTGATATC 
CTGAAAACCT GTGTACCAGA CCTAAGTGAC AAGAGACTGG AAGTGGTTGG GCTGGAGGGT 
GCAATTGAGA TGGGCCAAAT CTATACCAAG CTCAAGAATG CTGGACGTCG GCTGGCCAAG 
TGCTCTGAGA TCACCTTCCA CACCACAAAA ACCCTTCCCA TGCAAATTGA CGGAGAACCC 
TGGATGCAGA CGCCCTGTAC AATCAAGATC ACCCACAAGA ACCAGATGCC CATGCTCATG 
GGCCCACCCC CCCGCTCCAC CAATTTCTTT GGCTTCTTGA GCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DGKA ( NM_001351037.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001337966.1 Homo sapiens diacylglycerol kinase alpha (DGKA), transcript variant 9, mRNA.

Price & Availability↑

CloneID	OHu102467
Clone ID Related Accession (Same CDS sequence)	NM_001351036.1 , NM_001351037.1
Accession Version	NM_001351037.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1785bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-30
Organism	Homo sapiens(human)
Product	diacylglycerol kinase alpha isoform c
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC025162.48. On Apr 29, 2017 this sequence version replaced XM_017018909.1. Transcript Variant: This variant (9), as well as variant 8, encodes isoform c. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.168435.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2148093, SAMEA2148874 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGATGCGAG TGGCTGAATA CCTGGATTGG GATGTGTCTG AGCTGAGGCC GATTCTTCAG 
GAGATGATGA AAGAGATTGA CTATGATGGC AGTGGCTCTG TCTCTCAAGC TGAGTGGGTC 
CGGGCTGGGG CCACCACCGT GCCACTGCTA GTGCTGCTGG GTCTGGAGAT GACTCTGAAG 
GACGACGGAC AGCACATGTG GAGGCCCAAG AGGTTCCCCA GACCAGTCTA CTGCAATCTG 
TGCGAGTCAA GCATTGGTCT TGGCAAACAG GGACTGAGCT GTAACCTCTG TAAGTACACT 
GTTCACGACC AGTGTGCCAT GAAAGCCCTG CCTTGTGAAG TCAGCACCTA TGCCAAGTCT 
CGGAAGGACA TTGGTGTCCA ATCACATGTG TGGGTGCGAG GAGGCTGTGA GTCCGGGCGC 
TGCGACCGCT GTCAGAAAAA GATCCGGATC TACCACAGTC TGACCGGGCT GCATTGTGTA 
TGGTGCCACC TAGAGATCCA CGATGACTGC CTGCAAGCGG TGGGCCATGA GTGTGACTGT 
GGGCTGCTCC GGGATCACAT CCTGCCTCCA TCTTCCATCT ATCCCAGTGT CCTGGCCTCT 
GGACCGGATC GTAAAAATAG CAAAACAAGC CAGAAGACCA TGGATGATTT AAATTTGAGC 
ACCTCTGAGG CTCTGCGGAT TGACCCTGTT CCTAACACCC ACCCACTTCT CGTCTTTGTC 
AATCCTAAGA GTGGCGGGAA GCAGGGGCAA AGGGTGCTCT GGAAGTTCCA GTATATATTA 
AACCCTCGAC AGGTGTTCAA CCTCCTAAAG GATGGTCCTG AGATAGGGCT CCGATTATTC 
AAGGATGTTC CTGATAGCCG GATTTTGGTG TGTGGTGGAG ACGGCACAGT AGGCTGGATT 
CTAGAGACCA TTGACAAAGC TAACTTGCCA GTTTTGCCTC CTGTTGCTGT GTTGCCCCTG 
GGTACTGGAA ATGATCTGGC TCGATGCCTA AGATGGGGAG GAGGTTATGA AGGACAGAAT 
CTGGCAAAGA TCCTCAAGGA TTTAGAGATG AGTAAAGTGG TACATATGGA TCGATGGTCT 
GTGGAGGTGA TACCTCAACA AACTGAAGAA AAAAGTGACC CAGTCCCCTT TCAAATCATC 
AATAACTACT TCTCTATTGG CGTGGATGCC TCTATTGCTC ATCGATTCCA CATCATGCGA 
GAGAAATATC CGGAGAAGTT CAACAGCAGA ATGAAGAACA AGCTATGGTA CTTCGAATTT 
GCCACATCTG AATCCATCTT CTCAACATGC AAAAAGCTGG AGGAGTCTTT GACAGTTGAG 
ATCTGTGGGA AACCGCTGGA TCTGAGCAAC CTGTCCCTAG AAGGCATCGC AGTGCTAAAC 
ATCCCTAGCA TGCATGGTGG CTCCAACCTC TGGGGTGATA CCAGGAGACC CCATGGGGAT 
ATCTATGGGA TCAACCAGGC CTTAGGTGCT ACAGCTAAAG TCATCACCGA CCCTGATATC 
CTGAAAACCT GTGTACCAGA CCTAAGTGAC AAGAGACTGG AAGTGGTTGG GCTGGAGGGT 
GCAATTGAGA TGGGCCAAAT CTATACCAAG CTCAAGAATG CTGGACGTCG GCTGGCCAAG 
TGCTCTGAGA TCACCTTCCA CACCACAAAA ACCCTTCCCA TGCAAATTGA CGGAGAACCC 
TGGATGCAGA CGCCCTGTAC AATCAAGATC ACCCACAAGA ACCAGATGCC CATGCTCATG 
GGCCCACCCC CCCGCTCCAC CAATTTCTTT GGCTTCTTGA GCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001337966.1
CDS	647..2431
Translation	MMRVAEYLDWDVSELRPILQEMMKEIDYDGSGSVSQAEWVRAGATTVPLLVLLGLEMTLKDDGQHMWRPKRFPRPVYCNLCESSIGLGKQGLSCNLCKYTVHDQCAMKALPCEVSTYAKSRKDIGVQSHVWVRGGCESGRCDRCQKKIRIYHSLTGLHCVWCHLEIHDDCLQAVGHECDCGLLRDHILPPSSIYPSVLASGPDRKNSKTSQKTMDDLNLSTSEALRIDPVPNTHPLLVFVNPKSGGKQGQRVLWKFQYILNPRQVFNLLKDGPEIGLRLFKDVPDSRILVCGGDGTVGWILETIDKANLPVLPPVAVLPLGTGNDLARCLRWGGGYEGQNLAKILKDLEMSKVVHMDRWSVEVIPQQTEEKSDPVPFQIINNYFSIGVDASIAHRFHIMREKYPEKFNSRMKNKLWYFEFATSESIFSTCKKLEESLTVEICGKPLDLSNLSLEGIAVLNIPSMHGGSNLWGDTRRPHGDIYGINQALGATAKVITDPDILKTCVPDLSDKRLEVVGLEGAIEMGQIYTKLKNAGRRLAKCSEITFHTTKTLPMQIDGEPWMQTPCTIKITHKNQMPMLMGPPPRSTNFFGFLS

Target ORF information:

RefSeq Version	NM_001351037.1
Organism	Homo sapiens(human)
Definition	Homo sapiens diacylglycerol kinase alpha (DGKA), transcript variant 9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001351037.1

ORF Insert Sequence:

ATGATGCGAG TGGCTGAATA CCTGGATTGG GATGTGTCTG AGCTGAGGCC GATTCTTCAG 
GAGATGATGA AAGAGATTGA CTATGATGGC AGTGGCTCTG TCTCTCAAGC TGAGTGGGTC 
CGGGCTGGGG CCACCACCGT GCCACTGCTA GTGCTGCTGG GTCTGGAGAT GACTCTGAAG 
GACGACGGAC AGCACATGTG GAGGCCCAAG AGGTTCCCCA GACCAGTCTA CTGCAATCTG 
TGCGAGTCAA GCATTGGTCT TGGCAAACAG GGACTGAGCT GTAACCTCTG TAAGTACACT 
GTTCACGACC AGTGTGCCAT GAAAGCCCTG CCTTGTGAAG TCAGCACCTA TGCCAAGTCT 
CGGAAGGACA TTGGTGTCCA ATCACATGTG TGGGTGCGAG GAGGCTGTGA GTCCGGGCGC 
TGCGACCGCT GTCAGAAAAA GATCCGGATC TACCACAGTC TGACCGGGCT GCATTGTGTA 
TGGTGCCACC TAGAGATCCA CGATGACTGC CTGCAAGCGG TGGGCCATGA GTGTGACTGT 
GGGCTGCTCC GGGATCACAT CCTGCCTCCA TCTTCCATCT ATCCCAGTGT CCTGGCCTCT 
GGACCGGATC GTAAAAATAG CAAAACAAGC CAGAAGACCA TGGATGATTT AAATTTGAGC 
ACCTCTGAGG CTCTGCGGAT TGACCCTGTT CCTAACACCC ACCCACTTCT CGTCTTTGTC 
AATCCTAAGA GTGGCGGGAA GCAGGGGCAA AGGGTGCTCT GGAAGTTCCA GTATATATTA 
AACCCTCGAC AGGTGTTCAA CCTCCTAAAG GATGGTCCTG AGATAGGGCT CCGATTATTC 
AAGGATGTTC CTGATAGCCG GATTTTGGTG TGTGGTGGAG ACGGCACAGT AGGCTGGATT 
CTAGAGACCA TTGACAAAGC TAACTTGCCA GTTTTGCCTC CTGTTGCTGT GTTGCCCCTG 
GGTACTGGAA ATGATCTGGC TCGATGCCTA AGATGGGGAG GAGGTTATGA AGGACAGAAT 
CTGGCAAAGA TCCTCAAGGA TTTAGAGATG AGTAAAGTGG TACATATGGA TCGATGGTCT 
GTGGAGGTGA TACCTCAACA AACTGAAGAA AAAAGTGACC CAGTCCCCTT TCAAATCATC 
AATAACTACT TCTCTATTGG CGTGGATGCC TCTATTGCTC ATCGATTCCA CATCATGCGA 
GAGAAATATC CGGAGAAGTT CAACAGCAGA ATGAAGAACA AGCTATGGTA CTTCGAATTT 
GCCACATCTG AATCCATCTT CTCAACATGC AAAAAGCTGG AGGAGTCTTT GACAGTTGAG 
ATCTGTGGGA AACCGCTGGA TCTGAGCAAC CTGTCCCTAG AAGGCATCGC AGTGCTAAAC 
ATCCCTAGCA TGCATGGTGG CTCCAACCTC TGGGGTGATA CCAGGAGACC CCATGGGGAT 
ATCTATGGGA TCAACCAGGC CTTAGGTGCT ACAGCTAAAG TCATCACCGA CCCTGATATC 
CTGAAAACCT GTGTACCAGA CCTAAGTGAC AAGAGACTGG AAGTGGTTGG GCTGGAGGGT 
GCAATTGAGA TGGGCCAAAT CTATACCAAG CTCAAGAATG CTGGACGTCG GCTGGCCAAG 
TGCTCTGAGA TCACCTTCCA CACCACAAAA ACCCTTCCCA TGCAAATTGA CGGAGAACCC 
TGGATGCAGA CGCCCTGTAC AATCAAGATC ACCCACAAGA ACCAGATGCC CATGCTCATG 
GGCCCACCCC CCCGCTCCAC CAATTTCTTT GGCTTCTTGA GCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DGKA ( NM_001351035.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001337964.1 Homo sapiens diacylglycerol kinase alpha (DGKA), transcript variant 7, mRNA.

Price & Availability↑

CloneID	OHu46355
Clone ID Related Accession (Same CDS sequence)	NM_001351035.1
Accession Version	NM_001351035.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2322bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-30
Organism	Homo sapiens(human)
Product	diacylglycerol kinase alpha isoform b
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC025162.48. On Apr 29, 2017 this sequence version replaced XM_005268688.2. Transcript Variant: This variant (7) encodes the longest isoform (b). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.49634.1, SRR1803611.243910.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2144335 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGGTCAT GGCCAGGAAG ACGCGCTAAC GCAGCCTACC CTCTGAAGAG GTCCAAGCAA 
CGGAAGTACT ACTACGAAGC TGCCTTTCTG GCCATCCTTG AGAAAAATAG ACAGATGGCC 
AAGGAGAGGG GCCTAATAAG CCCCAGTGAT TTTGCCCAGC TGCAAAAATA CATGGAATAC 
TCCACCAAAA AGGTCAGTGA TGTCCTAAAG CTCTTCGAGG ATGGCGAGAT GGCTAAATAT 
GTCCAAGGAG ATGCCATTGG GTACGAGGGA TTCCAGCAAT TCCTGAAAAT CTATCTCGAA 
GTGGATAATG TTCCCAGACA CCTAAGCCTG GCACTGTTTC AATCCTTTGA GACTGGTCAC 
TGCTTAAATG AGACAAATGT GACAAAAGAT GTGGTGTGTC TCAATGATGT TTCCTGCTAC 
TTTTCCCTTC TGGAGGGTGG TCGGCCAGAA GACAAGTTAG AATTCACCTT CAAGCTGTAC 
GACACGGACA GAAATGGGAT CCTGGACAGC TCAGAAGTGG ACAAAATTAT CCTACAGATG 
ATGCGAGTGG CTGAATACCT GGATTGGGAT GTGTCTGAGC TGAGGCCGAT TCTTCAGGAG 
ATGATGAAAG AGATTGACTA TGATGGCAGT GGCTCTGTCT CTCAAGCTGA GTGGGTCCGG 
GCTGGGGCCA CCACCGTGCC ACTGCTAGTG CTGCTGGGTC TGGAGATGAC TCTGAAGGAC 
GACGGACAGC ACATGTGGAG GCCCAAGAGG TTCCCCAGAC CAGTCTACTG CAATCTGTGC 
GAGTCAAGCA TTGGTCTTGG CAAACAGGGA CTGAGCTGTA ACCTCTGTAA GTACACTGTT 
CACGACCAGT GTGCCATGAA AGCCCTGCCT TGTGAAGTCA GCACCTATGC CAAGTCTCGG 
AAGGACATTG GTGTCCAATC ACATGTGTGG GTGCGAGGAG GCTGTGAGTC CGGGCGCTGC 
GACCGCTGTC AGAAAAAGAT CCGGATCTAC CACAGTCTGA CCGGGCTGCA TTGTGTATGG 
TGCCACCTAG AGATCCACGA TGACTGCCTG CAAGCGGTGG GCCATGAGTG TGACTGTGGG 
CTGCTCCGGG ATCACATCCT GCCTCCATCT TCCATCTATC CCAGTGTCCT GGCCTCTGGA 
CCGGATCGTA AAAATAGCAA AACAAGCCAG AAGACCATGG ATGATTTAAA TTTGAGCACC 
TCTGAGGCTC TGCGGATTGA CCCTGTTCCT AACACCCACC CACTTCTCGT CTTTGTCAAT 
CCTAAGAGTG GCGGGAAGCA GGGGCAAAGG GTGCTCTGGA AGTTCCAGTA TATATTAAAC 
CCTCGACAGG TGTTCAACCT CCTAAAGGAT GGTCCTGAGA TAGGGCTCCG ATTATTCAAG 
GATGTTCCTG ATAGCCGGAT TTTGGTGTGT GGTGGAGACG GCACAGTAGG CTGGATTCTA 
GAGACCATTG ACAAAGCTAA CTTGCCAGTT TTGCCTCCTG TTGCTGTGTT GCCCCTGGGT 
ACTGGAAATG ATCTGGCTCG ATGCCTAAGA TGGGGAGGAG GTTATGAAGG ACAGAATCTG 
GCAAAGATCC TCAAGGATTT AGAGATGAGT AAAGTGGTAC ATATGGATCG ATGGTCTGTG 
GAGGTGATAC CTCAACAAAC TGAAGAAAAA AGTGACCCAG TCCCCTTTCA AATCATCAAT 
AACTACTTCT CTATTGGCGT GGATGCCTCT ATTGCTCATC GATTCCACAT CATGCGAGAG 
AAATATCCGG AGAAGTTCAA CAGCAGAATG AAGAACAAGC TATGGTACTT CGAATTTGCC 
ACATCTGAAT CCATCTTCTC AACATGCAAA AAGCTGGAGG AGTCTTTGAC AGTTGAGATC 
TGTGGGAAAC CGCTGGATCT GAGCAACCTG TCCCTAGAAG GCATCGCAGT GCTAAACATC 
CCTAGCATGC ATGGTGGCTC CAACCTCTGG GGTGATACCA GGAGACCCCA TGGGGATATC 
TATGGGATCA ACCAGGCCTT AGGTGCTACA GCTAAAGTCA TCACCGACCC TGATATCCTG 
AAAACCTGTG TACCAGACCT AAGTGACAAG AGACTGGAAG TGGTTGGGCT GGAGGGTGCA 
ATTGAGATGG GCCAAATCTA TACCAAGCTC AAGAATGCTG GACGTCGGCT GGCCAAGTGC 
TCTGAGATCA CCTTCCACAC CACAAAAACC CTTCCCATGC AAATTGACGG AGAACCCTGG 
ATGCAGACGC CCTGTACAAT CAAGATCACC CACAAGAACC AGATGCCCAT GCTCATGGGC 
CCACCCCCCC GCTCCACCAA TTTCTTTGGC TTCTTGAGCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001337964.1
CDS	93..2414
Translation	MGSWPGRRANAAYPLKRSKQRKYYYEAAFLAILEKNRQMAKERGLISPSDFAQLQKYMEYSTKKVSDVLKLFEDGEMAKYVQGDAIGYEGFQQFLKIYLEVDNVPRHLSLALFQSFETGHCLNETNVTKDVVCLNDVSCYFSLLEGGRPEDKLEFTFKLYDTDRNGILDSSEVDKIILQMMRVAEYLDWDVSELRPILQEMMKEIDYDGSGSVSQAEWVRAGATTVPLLVLLGLEMTLKDDGQHMWRPKRFPRPVYCNLCESSIGLGKQGLSCNLCKYTVHDQCAMKALPCEVSTYAKSRKDIGVQSHVWVRGGCESGRCDRCQKKIRIYHSLTGLHCVWCHLEIHDDCLQAVGHECDCGLLRDHILPPSSIYPSVLASGPDRKNSKTSQKTMDDLNLSTSEALRIDPVPNTHPLLVFVNPKSGGKQGQRVLWKFQYILNPRQVFNLLKDGPEIGLRLFKDVPDSRILVCGGDGTVGWILETIDKANLPVLPPVAVLPLGTGNDLARCLRWGGGYEGQNLAKILKDLEMSKVVHMDRWSVEVIPQQTEEKSDPVPFQIINNYFSIGVDASIAHRFHIMREKYPEKFNSRMKNKLWYFEFATSESIFSTCKKLEESLTVEICGKPLDLSNLSLEGIAVLNIPSMHGGSNLWGDTRRPHGDIYGINQALGATAKVITDPDILKTCVPDLSDKRLEVVGLEGAIEMGQIYTKLKNAGRRLAKCSEITFHTTKTLPMQIDGEPWMQTPCTIKITHKNQMPMLMGPPPRSTNFFGFLS

Target ORF information:

RefSeq Version	NM_001351035.1
Organism	Homo sapiens(human)
Definition	Homo sapiens diacylglycerol kinase alpha (DGKA), transcript variant 7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001351035.1

ORF Insert Sequence:

ATGGGGTCAT GGCCAGGAAG ACGCGCTAAC GCAGCCTACC CTCTGAAGAG GTCCAAGCAA 
CGGAAGTACT ACTACGAAGC TGCCTTTCTG GCCATCCTTG AGAAAAATAG ACAGATGGCC 
AAGGAGAGGG GCCTAATAAG CCCCAGTGAT TTTGCCCAGC TGCAAAAATA CATGGAATAC 
TCCACCAAAA AGGTCAGTGA TGTCCTAAAG CTCTTCGAGG ATGGCGAGAT GGCTAAATAT 
GTCCAAGGAG ATGCCATTGG GTACGAGGGA TTCCAGCAAT TCCTGAAAAT CTATCTCGAA 
GTGGATAATG TTCCCAGACA CCTAAGCCTG GCACTGTTTC AATCCTTTGA GACTGGTCAC 
TGCTTAAATG AGACAAATGT GACAAAAGAT GTGGTGTGTC TCAATGATGT TTCCTGCTAC 
TTTTCCCTTC TGGAGGGTGG TCGGCCAGAA GACAAGTTAG AATTCACCTT CAAGCTGTAC 
GACACGGACA GAAATGGGAT CCTGGACAGC TCAGAAGTGG ACAAAATTAT CCTACAGATG 
ATGCGAGTGG CTGAATACCT GGATTGGGAT GTGTCTGAGC TGAGGCCGAT TCTTCAGGAG 
ATGATGAAAG AGATTGACTA TGATGGCAGT GGCTCTGTCT CTCAAGCTGA GTGGGTCCGG 
GCTGGGGCCA CCACCGTGCC ACTGCTAGTG CTGCTGGGTC TGGAGATGAC TCTGAAGGAC 
GACGGACAGC ACATGTGGAG GCCCAAGAGG TTCCCCAGAC CAGTCTACTG CAATCTGTGC 
GAGTCAAGCA TTGGTCTTGG CAAACAGGGA CTGAGCTGTA ACCTCTGTAA GTACACTGTT 
CACGACCAGT GTGCCATGAA AGCCCTGCCT TGTGAAGTCA GCACCTATGC CAAGTCTCGG 
AAGGACATTG GTGTCCAATC ACATGTGTGG GTGCGAGGAG GCTGTGAGTC CGGGCGCTGC 
GACCGCTGTC AGAAAAAGAT CCGGATCTAC CACAGTCTGA CCGGGCTGCA TTGTGTATGG 
TGCCACCTAG AGATCCACGA TGACTGCCTG CAAGCGGTGG GCCATGAGTG TGACTGTGGG 
CTGCTCCGGG ATCACATCCT GCCTCCATCT TCCATCTATC CCAGTGTCCT GGCCTCTGGA 
CCGGATCGTA AAAATAGCAA AACAAGCCAG AAGACCATGG ATGATTTAAA TTTGAGCACC 
TCTGAGGCTC TGCGGATTGA CCCTGTTCCT AACACCCACC CACTTCTCGT CTTTGTCAAT 
CCTAAGAGTG GCGGGAAGCA GGGGCAAAGG GTGCTCTGGA AGTTCCAGTA TATATTAAAC 
CCTCGACAGG TGTTCAACCT CCTAAAGGAT GGTCCTGAGA TAGGGCTCCG ATTATTCAAG 
GATGTTCCTG ATAGCCGGAT TTTGGTGTGT GGTGGAGACG GCACAGTAGG CTGGATTCTA 
GAGACCATTG ACAAAGCTAA CTTGCCAGTT TTGCCTCCTG TTGCTGTGTT GCCCCTGGGT 
ACTGGAAATG ATCTGGCTCG ATGCCTAAGA TGGGGAGGAG GTTATGAAGG ACAGAATCTG 
GCAAAGATCC TCAAGGATTT AGAGATGAGT AAAGTGGTAC ATATGGATCG ATGGTCTGTG 
GAGGTGATAC CTCAACAAAC TGAAGAAAAA AGTGACCCAG TCCCCTTTCA AATCATCAAT 
AACTACTTCT CTATTGGCGT GGATGCCTCT ATTGCTCATC GATTCCACAT CATGCGAGAG 
AAATATCCGG AGAAGTTCAA CAGCAGAATG AAGAACAAGC TATGGTACTT CGAATTTGCC 
ACATCTGAAT CCATCTTCTC AACATGCAAA AAGCTGGAGG AGTCTTTGAC AGTTGAGATC 
TGTGGGAAAC CGCTGGATCT GAGCAACCTG TCCCTAGAAG GCATCGCAGT GCTAAACATC 
CCTAGCATGC ATGGTGGCTC CAACCTCTGG GGTGATACCA GGAGACCCCA TGGGGATATC 
TATGGGATCA ACCAGGCCTT AGGTGCTACA GCTAAAGTCA TCACCGACCC TGATATCCTG 
AAAACCTGTG TACCAGACCT AAGTGACAAG AGACTGGAAG TGGTTGGGCT GGAGGGTGCA 
ATTGAGATGG GCCAAATCTA TACCAAGCTC AAGAATGCTG GACGTCGGCT GGCCAAGTGC 
TCTGAGATCA CCTTCCACAC CACAAAAACC CTTCCCATGC AAATTGACGG AGAACCCTGG 
ATGCAGACGC CCTGTACAAT CAAGATCACC CACAAGAACC AGATGCCCAT GCTCATGGGC 
CCACCCCCCC GCTCCACCAA TTTCTTTGGC TTCTTGAGCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DGKA ( XM_017018900.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016874389.1 Homo sapiens diacylglycerol kinase alpha (DGKA), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu83567
Clone ID Related Accession (Same CDS sequence)	XM_017018900.1
Accession Version	XM_017018900.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2676bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	diacylglycerol kinase alpha isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGTCAT GGCCAGGAAG ACGCGCTAAC GCAGCCTACC CTCTGAAGAG GTCCAAGCAA 
CGGAAGTACT ACTACGAAGC TGCCTTTCTG GCCATCCTTG AGAAAAATAG ACAGATGGCC 
AAGGAGAGGG GCCTAATAAG CCCCAGTGAT TTTGCCCAGC TGCAAAAATA CATGGAATAC 
TCCACCAAAA AGGTCAGTGA TGTCCTAAAG CTCTTCGAGG ATGGCGAGAT GGCTAAATAT 
GTCCAAGGAG ATGCCATTGG GTACGAGGGA TTCCAGCAAT TCCTGAAAAT CTATCTCGAA 
GTGGATAATG TTCCCAGACA CCTAAGCCTG GCACTGTTTC AATCCTTTGA GACTGGTCAC 
TGCTTAAATG AGACAAATGT GACAAAAGAT GTGGTGTGTC TCAATGATGT TTCCTGCTAC 
TTTTCCCTTC TGGAGGGTGG TCGGCCAGAA GACAAGTTAG AATTCACCTT CAAGCTGTAC 
GACACGGACA GAAATGGGAT CCTGGACAGC TCAGTGAGTT GGGGACCCTG TATGCTGGGC 
AAGGGAATAT GTGTTAATTT GTCTGCACCC TCCTGCCCCA ACTCTTCCAG GGTCTTAAGA 
AACAGAAGAG GATGGGGGGA GAGGTTGAGT CCTCTAGGAC TAAATTTGAA AGTAAGTCCC 
AAGCTCTTGA CCCCTCAAAG AGAATGCTGG ATACATGAAT TGGGTCTGCC TTACAAACAT 
ACAAACACAT TTATTTACTT GGCAGACATA CCTGAGAATG AGAGGTGTGA ATCTGGGGTG 
GAACCCAGGA ACACTGATTT CTGGAAAAGA GTTTGGATGG TGGTAGTAAA GGGGATATGG 
CTGTGGCTCT TGCCCTTTTT GCTCCAGGAA GTGGACAAAA TTATCCTACA GATGATGCGA 
GTGGCTGAAT ACCTGGATTG GGATGTGTCT GAGCTGAGGC CGATTCTTCA GGAGATGATG 
AAAGAGATTG ACTATGATGG CAGTGGCTCT GTCTCTCAAG CTGAGTGGGT CCGGGCTGGG 
GCCACCACCG TGCCACTGCT AGTGCTGCTG GGTCTGGAGA TGACTCTGAA GGACGACGGA 
CAGCACATGT GGAGGCCCAA GAGGTTCCCC AGACCAGTCT ACTGCAATCT GTGCGAGTCA 
AGCATTGGTC TTGGCAAACA GGGACTGAGC TGTAACCTCT GTAAGTACAC TGTTCACGAC 
CAGTGTGCCA TGAAAGCCCT GCCTTGTGAA GTCAGCACCT ATGCCAAGTC TCGGAAGGAC 
ATTGGTGTCC AATCACATGT GTGGGTGCGA GGAGGCTGTG AGTCCGGGCG CTGCGACCGC 
TGTCAGAAAA AGATCCGGAT CTACCACAGT CTGACCGGGC TGCATTGTGT ATGGTGCCAC 
CTAGAGATCC ACGATGACTG CCTGCAAGCG GTGGGCCATG AGTGTGACTG TGGGCTGCTC 
CGGGATCACA TCCTGCCTCC ATCTTCCATC TATCCCAGTG TCCTGGCCTC TGGACCGGAT 
CGTAAAAATA GCAAAACAAG CCAGAAGACC ATGGATGATT TAAATTTGAG CACCTCTGAG 
GCTCTGCGGA TTGACCCTGT TCCTAACACC CACCCACTTC TCGTCTTTGT CAATCCTAAG 
AGTGGCGGGA AGCAGGGGCA AAGGGTGCTC TGGAAGTTCC AGTATATATT AAACCCTCGA 
CAGGTGTTCA ACCTCCTAAA GGATGGTCCT GAGATAGGGC TCCGATTATT CAAGGATGTT 
CCTGATAGCC GGATTTTGGT GTGTGGTGGA GACGGCACAG TAGGCTGGAT TCTAGAGACC 
ATTGACAAAG CTAACTTGCC AGTTTTGCCT CCTGTTGCTG TGTTGCCCCT GGGTACTGGA 
AATGATCTGG CTCGATGCCT AAGATGGGGA GGAGGTTATG AAGGACAGAA TCTGGCAAAG 
ATCCTCAAGG ATTTAGAGAT GAGTAAAGTG GTACATATGG ATCGATGGTC TGTGGAGGTG 
ATACCTCAAC AAACTGAAGA AAAAAGTGAC CCAGTCCCCT TTCAAATCAT CAATAACTAC 
TTCTCTATTG GCGTGGATGC CTCTATTGCT CATCGATTCC ACATCATGCG AGAGAAATAT 
CCGGAGAAGT TCAACAGCAG AATGAAGAAC AAGCTATGGT ACTTCGAATT TGCCACATCT 
GAATCCATCT TCTCAACATG CAAAAAGCTG GAGGAGTCTT TGACAGTTGA GATCTGTGGG 
AAACCGCTGG ATCTGAGCAA CCTGTCCCTA GAAGGCATCG CAGTGCTAAA CATCCCTAGC 
ATGCATGGTG GCTCCAACCT CTGGGGTGAT ACCAGGAGAC CCCATGGGGA TATCTATGGG 
ATCAACCAGG CCTTAGGTGC TACAGCTAAA GTCATCACCG ACCCTGATAT CCTGAAAACC 
TGTGTACCAG ACCTAAGTGA CAAGAGACTG GAAGTGGTTG GGCTGGAGGG TGCAATTGAG 
ATGGGCCAAA TCTATACCAA GCTCAAGAAT GCTGGACGTC GGCTGGCCAA GTGCTCTGAG 
ATCACCTTCC ACACCACAAA AACCCTTCCC ATGCAAATTG ACGGAGAACC CTGGATGCAG 
ACGCCCTGTA CAATCAAGAT CACCCACAAG AACCAGATGC CCATGCTCAT GGGCCCACCC 
CCCCGCTCCA CCAATTTCTT TGGCTTCTTG AGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016874389.1
CDS	93..2768
Translation	MGSWPGRRANAAYPLKRSKQRKYYYEAAFLAILEKNRQMAKERGLISPSDFAQLQKYMEYSTKKVSDVLKLFEDGEMAKYVQGDAIGYEGFQQFLKIYLEVDNVPRHLSLALFQSFETGHCLNETNVTKDVVCLNDVSCYFSLLEGGRPEDKLEFTFKLYDTDRNGILDSSVSWGPCMLGKGICVNLSAPSCPNSSRVLRNRRGWGERLSPLGLNLKVSPKLLTPQRECWIHELGLPYKHTNTFIYLADIPENERCESGVEPRNTDFWKRVWMVVVKGIWLWLLPFLLQEVDKIILQMMRVAEYLDWDVSELRPILQEMMKEIDYDGSGSVSQAEWVRAGATTVPLLVLLGLEMTLKDDGQHMWRPKRFPRPVYCNLCESSIGLGKQGLSCNLCKYTVHDQCAMKALPCEVSTYAKSRKDIGVQSHVWVRGGCESGRCDRCQKKIRIYHSLTGLHCVWCHLEIHDDCLQAVGHECDCGLLRDHILPPSSIYPSVLASGPDRKNSKTSQKTMDDLNLSTSEALRIDPVPNTHPLLVFVNPKSGGKQGQRVLWKFQYILNPRQVFNLLKDGPEIGLRLFKDVPDSRILVCGGDGTVGWILETIDKANLPVLPPVAVLPLGTGNDLARCLRWGGGYEGQNLAKILKDLEMSKVVHMDRWSVEVIPQQTEEKSDPVPFQIINNYFSIGVDASIAHRFHIMREKYPEKFNSRMKNKLWYFEFATSESIFSTCKKLEESLTVEICGKPLDLSNLSLEGIAVLNIPSMHGGSNLWGDTRRPHGDIYGINQALGATAKVITDPDILKTCVPDLSDKRLEVVGLEGAIEMGQIYTKLKNAGRRLAKCSEITFHTTKTLPMQIDGEPWMQTPCTIKITHKNQMPMLMGPPPRSTNFFGFLS

Target ORF information:

RefSeq Version	XM_017018900.1
Organism	Homo sapiens(human)
Definition	Homo sapiens diacylglycerol kinase alpha (DGKA), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017018900.1

ORF Insert Sequence:

ATGGGGTCAT GGCCAGGAAG ACGCGCTAAC GCAGCCTACC CTCTGAAGAG GTCCAAGCAA 
CGGAAGTACT ACTACGAAGC TGCCTTTCTG GCCATCCTTG AGAAAAATAG ACAGATGGCC 
AAGGAGAGGG GCCTAATAAG CCCCAGTGAT TTTGCCCAGC TGCAAAAATA CATGGAATAC 
TCCACCAAAA AGGTCAGTGA TGTCCTAAAG CTCTTCGAGG ATGGCGAGAT GGCTAAATAT 
GTCCAAGGAG ATGCCATTGG GTACGAGGGA TTCCAGCAAT TCCTGAAAAT CTATCTCGAA 
GTGGATAATG TTCCCAGACA CCTAAGCCTG GCACTGTTTC AATCCTTTGA GACTGGTCAC 
TGCTTAAATG AGACAAATGT GACAAAAGAT GTGGTGTGTC TCAATGATGT TTCCTGCTAC 
TTTTCCCTTC TGGAGGGTGG TCGGCCAGAA GACAAGTTAG AATTCACCTT CAAGCTGTAC 
GACACGGACA GAAATGGGAT CCTGGACAGC TCAGTGAGTT GGGGACCCTG TATGCTGGGC 
AAGGGAATAT GTGTTAATTT GTCTGCACCC TCCTGCCCCA ACTCTTCCAG GGTCTTAAGA 
AACAGAAGAG GATGGGGGGA GAGGTTGAGT CCTCTAGGAC TAAATTTGAA AGTAAGTCCC 
AAGCTCTTGA CCCCTCAAAG AGAATGCTGG ATACATGAAT TGGGTCTGCC TTACAAACAT 
ACAAACACAT TTATTTACTT GGCAGACATA CCTGAGAATG AGAGGTGTGA ATCTGGGGTG 
GAACCCAGGA ACACTGATTT CTGGAAAAGA GTTTGGATGG TGGTAGTAAA GGGGATATGG 
CTGTGGCTCT TGCCCTTTTT GCTCCAGGAA GTGGACAAAA TTATCCTACA GATGATGCGA 
GTGGCTGAAT ACCTGGATTG GGATGTGTCT GAGCTGAGGC CGATTCTTCA GGAGATGATG 
AAAGAGATTG ACTATGATGG CAGTGGCTCT GTCTCTCAAG CTGAGTGGGT CCGGGCTGGG 
GCCACCACCG TGCCACTGCT AGTGCTGCTG GGTCTGGAGA TGACTCTGAA GGACGACGGA 
CAGCACATGT GGAGGCCCAA GAGGTTCCCC AGACCAGTCT ACTGCAATCT GTGCGAGTCA 
AGCATTGGTC TTGGCAAACA GGGACTGAGC TGTAACCTCT GTAAGTACAC TGTTCACGAC 
CAGTGTGCCA TGAAAGCCCT GCCTTGTGAA GTCAGCACCT ATGCCAAGTC TCGGAAGGAC 
ATTGGTGTCC AATCACATGT GTGGGTGCGA GGAGGCTGTG AGTCCGGGCG CTGCGACCGC 
TGTCAGAAAA AGATCCGGAT CTACCACAGT CTGACCGGGC TGCATTGTGT ATGGTGCCAC 
CTAGAGATCC ACGATGACTG CCTGCAAGCG GTGGGCCATG AGTGTGACTG TGGGCTGCTC 
CGGGATCACA TCCTGCCTCC ATCTTCCATC TATCCCAGTG TCCTGGCCTC TGGACCGGAT 
CGTAAAAATA GCAAAACAAG CCAGAAGACC ATGGATGATT TAAATTTGAG CACCTCTGAG 
GCTCTGCGGA TTGACCCTGT TCCTAACACC CACCCACTTC TCGTCTTTGT CAATCCTAAG 
AGTGGCGGGA AGCAGGGGCA AAGGGTGCTC TGGAAGTTCC AGTATATATT AAACCCTCGA 
CAGGTGTTCA ACCTCCTAAA GGATGGTCCT GAGATAGGGC TCCGATTATT CAAGGATGTT 
CCTGATAGCC GGATTTTGGT GTGTGGTGGA GACGGCACAG TAGGCTGGAT TCTAGAGACC 
ATTGACAAAG CTAACTTGCC AGTTTTGCCT CCTGTTGCTG TGTTGCCCCT GGGTACTGGA 
AATGATCTGG CTCGATGCCT AAGATGGGGA GGAGGTTATG AAGGACAGAA TCTGGCAAAG 
ATCCTCAAGG ATTTAGAGAT GAGTAAAGTG GTACATATGG ATCGATGGTC TGTGGAGGTG 
ATACCTCAAC AAACTGAAGA AAAAAGTGAC CCAGTCCCCT TTCAAATCAT CAATAACTAC 
TTCTCTATTG GCGTGGATGC CTCTATTGCT CATCGATTCC ACATCATGCG AGAGAAATAT 
CCGGAGAAGT TCAACAGCAG AATGAAGAAC AAGCTATGGT ACTTCGAATT TGCCACATCT 
GAATCCATCT TCTCAACATG CAAAAAGCTG GAGGAGTCTT TGACAGTTGA GATCTGTGGG 
AAACCGCTGG ATCTGAGCAA CCTGTCCCTA GAAGGCATCG CAGTGCTAAA CATCCCTAGC 
ATGCATGGTG GCTCCAACCT CTGGGGTGAT ACCAGGAGAC CCCATGGGGA TATCTATGGG 
ATCAACCAGG CCTTAGGTGC TACAGCTAAA GTCATCACCG ACCCTGATAT CCTGAAAACC 
TGTGTACCAG ACCTAAGTGA CAAGAGACTG GAAGTGGTTG GGCTGGAGGG TGCAATTGAG 
ATGGGCCAAA TCTATACCAA GCTCAAGAAT GCTGGACGTC GGCTGGCCAA GTGCTCTGAG 
ATCACCTTCC ACACCACAAA AACCCTTCCC ATGCAAATTG ACGGAGAACC CTGGATGCAG 
ACGCCCTGTA CAATCAAGAT CACCCACAAG AACCAGATGC CCATGCTCAT GGGCCCACCC 
CCCCGCTCCA CCAATTTCTT TGGCTTCTTG AGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DGKA ( XM_017018903.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016874392.1 Homo sapiens diacylglycerol kinase alpha (DGKA), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OHu83568
Clone ID Related Accession (Same CDS sequence)	XM_017018903.1 , XM_017018904.1 , XM_017018901.2 , XM_017018907.1 , XM_017018906.1 , XM_017018905.1 , XM_017018906.2 , XM_017018901.1 , XM_017018902.1
Accession Version	XM_017018903.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2562bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	diacylglycerol kinase alpha isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAGG AGAGGGGCCT AATAAGCCCC AGTGATTTTG CCCAGCTGCA AAAATACATG 
GAATACTCCA CCAAAAAGGT CAGTGATGTC CTAAAGCTCT TCGAGGATGG CGAGATGGCT 
AAATATGTCC AAGGAGATGC CATTGGGTAC GAGGGATTCC AGCAATTCCT GAAAATCTAT 
CTCGAAGTGG ATAATGTTCC CAGACACCTA AGCCTGGCAC TGTTTCAATC CTTTGAGACT 
GGTCACTGCT TAAATGAGAC AAATGTGACA AAAGATGTGG TGTGTCTCAA TGATGTTTCC 
TGCTACTTTT CCCTTCTGGA GGGTGGTCGG CCAGAAGACA AGTTAGAATT CACCTTCAAG 
CTGTACGACA CGGACAGAAA TGGGATCCTG GACAGCTCAG TGAGTTGGGG ACCCTGTATG 
CTGGGCAAGG GAATATGTGT TAATTTGTCT GCACCCTCCT GCCCCAACTC TTCCAGGGTC 
TTAAGAAACA GAAGAGGATG GGGGGAGAGG TTGAGTCCTC TAGGACTAAA TTTGAAAGTA 
AGTCCCAAGC TCTTGACCCC TCAAAGAGAA TGCTGGATAC ATGAATTGGG TCTGCCTTAC 
AAACATACAA ACACATTTAT TTACTTGGCA GACATACCTG AGAATGAGAG GTGTGAATCT 
GGGGTGGAAC CCAGGAACAC TGATTTCTGG AAAAGAGTTT GGATGGTGGT AGTAAAGGGG 
ATATGGCTGT GGCTCTTGCC CTTTTTGCTC CAGGAAGTGG ACAAAATTAT CCTACAGATG 
ATGCGAGTGG CTGAATACCT GGATTGGGAT GTGTCTGAGC TGAGGCCGAT TCTTCAGGAG 
ATGATGAAAG AGATTGACTA TGATGGCAGT GGCTCTGTCT CTCAAGCTGA GTGGGTCCGG 
GCTGGGGCCA CCACCGTGCC ACTGCTAGTG CTGCTGGGTC TGGAGATGAC TCTGAAGGAC 
GACGGACAGC ACATGTGGAG GCCCAAGAGG TTCCCCAGAC CAGTCTACTG CAATCTGTGC 
GAGTCAAGCA TTGGTCTTGG CAAACAGGGA CTGAGCTGTA ACCTCTGTAA GTACACTGTT 
CACGACCAGT GTGCCATGAA AGCCCTGCCT TGTGAAGTCA GCACCTATGC CAAGTCTCGG 
AAGGACATTG GTGTCCAATC ACATGTGTGG GTGCGAGGAG GCTGTGAGTC CGGGCGCTGC 
GACCGCTGTC AGAAAAAGAT CCGGATCTAC CACAGTCTGA CCGGGCTGCA TTGTGTATGG 
TGCCACCTAG AGATCCACGA TGACTGCCTG CAAGCGGTGG GCCATGAGTG TGACTGTGGG 
CTGCTCCGGG ATCACATCCT GCCTCCATCT TCCATCTATC CCAGTGTCCT GGCCTCTGGA 
CCGGATCGTA AAAATAGCAA AACAAGCCAG AAGACCATGG ATGATTTAAA TTTGAGCACC 
TCTGAGGCTC TGCGGATTGA CCCTGTTCCT AACACCCACC CACTTCTCGT CTTTGTCAAT 
CCTAAGAGTG GCGGGAAGCA GGGGCAAAGG GTGCTCTGGA AGTTCCAGTA TATATTAAAC 
CCTCGACAGG TGTTCAACCT CCTAAAGGAT GGTCCTGAGA TAGGGCTCCG ATTATTCAAG 
GATGTTCCTG ATAGCCGGAT TTTGGTGTGT GGTGGAGACG GCACAGTAGG CTGGATTCTA 
GAGACCATTG ACAAAGCTAA CTTGCCAGTT TTGCCTCCTG TTGCTGTGTT GCCCCTGGGT 
ACTGGAAATG ATCTGGCTCG ATGCCTAAGA TGGGGAGGAG GTTATGAAGG ACAGAATCTG 
GCAAAGATCC TCAAGGATTT AGAGATGAGT AAAGTGGTAC ATATGGATCG ATGGTCTGTG 
GAGGTGATAC CTCAACAAAC TGAAGAAAAA AGTGACCCAG TCCCCTTTCA AATCATCAAT 
AACTACTTCT CTATTGGCGT GGATGCCTCT ATTGCTCATC GATTCCACAT CATGCGAGAG 
AAATATCCGG AGAAGTTCAA CAGCAGAATG AAGAACAAGC TATGGTACTT CGAATTTGCC 
ACATCTGAAT CCATCTTCTC AACATGCAAA AAGCTGGAGG AGTCTTTGAC AGTTGAGATC 
TGTGGGAAAC CGCTGGATCT GAGCAACCTG TCCCTAGAAG GCATCGCAGT GCTAAACATC 
CCTAGCATGC ATGGTGGCTC CAACCTCTGG GGTGATACCA GGAGACCCCA TGGGGATATC 
TATGGGATCA ACCAGGCCTT AGGTGCTACA GCTAAAGTCA TCACCGACCC TGATATCCTG 
AAAACCTGTG TACCAGACCT AAGTGACAAG AGACTGGAAG TGGTTGGGCT GGAGGGTGCA 
ATTGAGATGG GCCAAATCTA TACCAAGCTC AAGAATGCTG GACGTCGGCT GGCCAAGTGC 
TCTGAGATCA CCTTCCACAC CACAAAAACC CTTCCCATGC AAATTGACGG AGAACCCTGG 
ATGCAGACGC CCTGTACAAT CAAGATCACC CACAAGAACC AGATGCCCAT GCTCATGGGC 
CCACCCCCCC GCTCCACCAA TTTCTTTGGC TTCTTGAGCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016874392.1
CDS	375..2936
Translation	MAKERGLISPSDFAQLQKYMEYSTKKVSDVLKLFEDGEMAKYVQGDAIGYEGFQQFLKIYLEVDNVPRHLSLALFQSFETGHCLNETNVTKDVVCLNDVSCYFSLLEGGRPEDKLEFTFKLYDTDRNGILDSSVSWGPCMLGKGICVNLSAPSCPNSSRVLRNRRGWGERLSPLGLNLKVSPKLLTPQRECWIHELGLPYKHTNTFIYLADIPENERCESGVEPRNTDFWKRVWMVVVKGIWLWLLPFLLQEVDKIILQMMRVAEYLDWDVSELRPILQEMMKEIDYDGSGSVSQAEWVRAGATTVPLLVLLGLEMTLKDDGQHMWRPKRFPRPVYCNLCESSIGLGKQGLSCNLCKYTVHDQCAMKALPCEVSTYAKSRKDIGVQSHVWVRGGCESGRCDRCQKKIRIYHSLTGLHCVWCHLEIHDDCLQAVGHECDCGLLRDHILPPSSIYPSVLASGPDRKNSKTSQKTMDDLNLSTSEALRIDPVPNTHPLLVFVNPKSGGKQGQRVLWKFQYILNPRQVFNLLKDGPEIGLRLFKDVPDSRILVCGGDGTVGWILETIDKANLPVLPPVAVLPLGTGNDLARCLRWGGGYEGQNLAKILKDLEMSKVVHMDRWSVEVIPQQTEEKSDPVPFQIINNYFSIGVDASIAHRFHIMREKYPEKFNSRMKNKLWYFEFATSESIFSTCKKLEESLTVEICGKPLDLSNLSLEGIAVLNIPSMHGGSNLWGDTRRPHGDIYGINQALGATAKVITDPDILKTCVPDLSDKRLEVVGLEGAIEMGQIYTKLKNAGRRLAKCSEITFHTTKTLPMQIDGEPWMQTPCTIKITHKNQMPMLMGPPPRSTNFFGFLS

Target ORF information:

RefSeq Version	XM_017018903.1
Organism	Homo sapiens(human)
Definition	Homo sapiens diacylglycerol kinase alpha (DGKA), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017018903.1

ORF Insert Sequence:

ATGGCCAAGG AGAGGGGCCT AATAAGCCCC AGTGATTTTG CCCAGCTGCA AAAATACATG 
GAATACTCCA CCAAAAAGGT CAGTGATGTC CTAAAGCTCT TCGAGGATGG CGAGATGGCT 
AAATATGTCC AAGGAGATGC CATTGGGTAC GAGGGATTCC AGCAATTCCT GAAAATCTAT 
CTCGAAGTGG ATAATGTTCC CAGACACCTA AGCCTGGCAC TGTTTCAATC CTTTGAGACT 
GGTCACTGCT TAAATGAGAC AAATGTGACA AAAGATGTGG TGTGTCTCAA TGATGTTTCC 
TGCTACTTTT CCCTTCTGGA GGGTGGTCGG CCAGAAGACA AGTTAGAATT CACCTTCAAG 
CTGTACGACA CGGACAGAAA TGGGATCCTG GACAGCTCAG TGAGTTGGGG ACCCTGTATG 
CTGGGCAAGG GAATATGTGT TAATTTGTCT GCACCCTCCT GCCCCAACTC TTCCAGGGTC 
TTAAGAAACA GAAGAGGATG GGGGGAGAGG TTGAGTCCTC TAGGACTAAA TTTGAAAGTA 
AGTCCCAAGC TCTTGACCCC TCAAAGAGAA TGCTGGATAC ATGAATTGGG TCTGCCTTAC 
AAACATACAA ACACATTTAT TTACTTGGCA GACATACCTG AGAATGAGAG GTGTGAATCT 
GGGGTGGAAC CCAGGAACAC TGATTTCTGG AAAAGAGTTT GGATGGTGGT AGTAAAGGGG 
ATATGGCTGT GGCTCTTGCC CTTTTTGCTC CAGGAAGTGG ACAAAATTAT CCTACAGATG 
ATGCGAGTGG CTGAATACCT GGATTGGGAT GTGTCTGAGC TGAGGCCGAT TCTTCAGGAG 
ATGATGAAAG AGATTGACTA TGATGGCAGT GGCTCTGTCT CTCAAGCTGA GTGGGTCCGG 
GCTGGGGCCA CCACCGTGCC ACTGCTAGTG CTGCTGGGTC TGGAGATGAC TCTGAAGGAC 
GACGGACAGC ACATGTGGAG GCCCAAGAGG TTCCCCAGAC CAGTCTACTG CAATCTGTGC 
GAGTCAAGCA TTGGTCTTGG CAAACAGGGA CTGAGCTGTA ACCTCTGTAA GTACACTGTT 
CACGACCAGT GTGCCATGAA AGCCCTGCCT TGTGAAGTCA GCACCTATGC CAAGTCTCGG 
AAGGACATTG GTGTCCAATC ACATGTGTGG GTGCGAGGAG GCTGTGAGTC CGGGCGCTGC 
GACCGCTGTC AGAAAAAGAT CCGGATCTAC CACAGTCTGA CCGGGCTGCA TTGTGTATGG 
TGCCACCTAG AGATCCACGA TGACTGCCTG CAAGCGGTGG GCCATGAGTG TGACTGTGGG 
CTGCTCCGGG ATCACATCCT GCCTCCATCT TCCATCTATC CCAGTGTCCT GGCCTCTGGA 
CCGGATCGTA AAAATAGCAA AACAAGCCAG AAGACCATGG ATGATTTAAA TTTGAGCACC 
TCTGAGGCTC TGCGGATTGA CCCTGTTCCT AACACCCACC CACTTCTCGT CTTTGTCAAT 
CCTAAGAGTG GCGGGAAGCA GGGGCAAAGG GTGCTCTGGA AGTTCCAGTA TATATTAAAC 
CCTCGACAGG TGTTCAACCT CCTAAAGGAT GGTCCTGAGA TAGGGCTCCG ATTATTCAAG 
GATGTTCCTG ATAGCCGGAT TTTGGTGTGT GGTGGAGACG GCACAGTAGG CTGGATTCTA 
GAGACCATTG ACAAAGCTAA CTTGCCAGTT TTGCCTCCTG TTGCTGTGTT GCCCCTGGGT 
ACTGGAAATG ATCTGGCTCG ATGCCTAAGA TGGGGAGGAG GTTATGAAGG ACAGAATCTG 
GCAAAGATCC TCAAGGATTT AGAGATGAGT AAAGTGGTAC ATATGGATCG ATGGTCTGTG 
GAGGTGATAC CTCAACAAAC TGAAGAAAAA AGTGACCCAG TCCCCTTTCA AATCATCAAT 
AACTACTTCT CTATTGGCGT GGATGCCTCT ATTGCTCATC GATTCCACAT CATGCGAGAG 
AAATATCCGG AGAAGTTCAA CAGCAGAATG AAGAACAAGC TATGGTACTT CGAATTTGCC 
ACATCTGAAT CCATCTTCTC AACATGCAAA AAGCTGGAGG AGTCTTTGAC AGTTGAGATC 
TGTGGGAAAC CGCTGGATCT GAGCAACCTG TCCCTAGAAG GCATCGCAGT GCTAAACATC 
CCTAGCATGC ATGGTGGCTC CAACCTCTGG GGTGATACCA GGAGACCCCA TGGGGATATC 
TATGGGATCA ACCAGGCCTT AGGTGCTACA GCTAAAGTCA TCACCGACCC TGATATCCTG 
AAAACCTGTG TACCAGACCT AAGTGACAAG AGACTGGAAG TGGTTGGGCT GGAGGGTGCA 
ATTGAGATGG GCCAAATCTA TACCAAGCTC AAGAATGCTG GACGTCGGCT GGCCAAGTGC 
TCTGAGATCA CCTTCCACAC CACAAAAACC CTTCCCATGC AAATTGACGG AGAACCCTGG 
ATGCAGACGC CCTGTACAAT CAAGATCACC CACAAGAACC AGATGCCCAT GCTCATGGGC 
CCACCCCCCC GCTCCACCAA TTTCTTTGGC TTCTTGAGCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DGKA ( XM_017018904.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016874393.1 Homo sapiens diacylglycerol kinase alpha (DGKA), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OHu83568
Clone ID Related Accession (Same CDS sequence)	XM_017018903.1 , XM_017018904.1 , XM_017018901.2 , XM_017018907.1 , XM_017018906.1 , XM_017018905.1 , XM_017018906.2 , XM_017018901.1 , XM_017018902.1
Accession Version	XM_017018904.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2562bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	diacylglycerol kinase alpha isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAGG AGAGGGGCCT AATAAGCCCC AGTGATTTTG CCCAGCTGCA AAAATACATG 
GAATACTCCA CCAAAAAGGT CAGTGATGTC CTAAAGCTCT TCGAGGATGG CGAGATGGCT 
AAATATGTCC AAGGAGATGC CATTGGGTAC GAGGGATTCC AGCAATTCCT GAAAATCTAT 
CTCGAAGTGG ATAATGTTCC CAGACACCTA AGCCTGGCAC TGTTTCAATC CTTTGAGACT 
GGTCACTGCT TAAATGAGAC AAATGTGACA AAAGATGTGG TGTGTCTCAA TGATGTTTCC 
TGCTACTTTT CCCTTCTGGA GGGTGGTCGG CCAGAAGACA AGTTAGAATT CACCTTCAAG 
CTGTACGACA CGGACAGAAA TGGGATCCTG GACAGCTCAG TGAGTTGGGG ACCCTGTATG 
CTGGGCAAGG GAATATGTGT TAATTTGTCT GCACCCTCCT GCCCCAACTC TTCCAGGGTC 
TTAAGAAACA GAAGAGGATG GGGGGAGAGG TTGAGTCCTC TAGGACTAAA TTTGAAAGTA 
AGTCCCAAGC TCTTGACCCC TCAAAGAGAA TGCTGGATAC ATGAATTGGG TCTGCCTTAC 
AAACATACAA ACACATTTAT TTACTTGGCA GACATACCTG AGAATGAGAG GTGTGAATCT 
GGGGTGGAAC CCAGGAACAC TGATTTCTGG AAAAGAGTTT GGATGGTGGT AGTAAAGGGG 
ATATGGCTGT GGCTCTTGCC CTTTTTGCTC CAGGAAGTGG ACAAAATTAT CCTACAGATG 
ATGCGAGTGG CTGAATACCT GGATTGGGAT GTGTCTGAGC TGAGGCCGAT TCTTCAGGAG 
ATGATGAAAG AGATTGACTA TGATGGCAGT GGCTCTGTCT CTCAAGCTGA GTGGGTCCGG 
GCTGGGGCCA CCACCGTGCC ACTGCTAGTG CTGCTGGGTC TGGAGATGAC TCTGAAGGAC 
GACGGACAGC ACATGTGGAG GCCCAAGAGG TTCCCCAGAC CAGTCTACTG CAATCTGTGC 
GAGTCAAGCA TTGGTCTTGG CAAACAGGGA CTGAGCTGTA ACCTCTGTAA GTACACTGTT 
CACGACCAGT GTGCCATGAA AGCCCTGCCT TGTGAAGTCA GCACCTATGC CAAGTCTCGG 
AAGGACATTG GTGTCCAATC ACATGTGTGG GTGCGAGGAG GCTGTGAGTC CGGGCGCTGC 
GACCGCTGTC AGAAAAAGAT CCGGATCTAC CACAGTCTGA CCGGGCTGCA TTGTGTATGG 
TGCCACCTAG AGATCCACGA TGACTGCCTG CAAGCGGTGG GCCATGAGTG TGACTGTGGG 
CTGCTCCGGG ATCACATCCT GCCTCCATCT TCCATCTATC CCAGTGTCCT GGCCTCTGGA 
CCGGATCGTA AAAATAGCAA AACAAGCCAG AAGACCATGG ATGATTTAAA TTTGAGCACC 
TCTGAGGCTC TGCGGATTGA CCCTGTTCCT AACACCCACC CACTTCTCGT CTTTGTCAAT 
CCTAAGAGTG GCGGGAAGCA GGGGCAAAGG GTGCTCTGGA AGTTCCAGTA TATATTAAAC 
CCTCGACAGG TGTTCAACCT CCTAAAGGAT GGTCCTGAGA TAGGGCTCCG ATTATTCAAG 
GATGTTCCTG ATAGCCGGAT TTTGGTGTGT GGTGGAGACG GCACAGTAGG CTGGATTCTA 
GAGACCATTG ACAAAGCTAA CTTGCCAGTT TTGCCTCCTG TTGCTGTGTT GCCCCTGGGT 
ACTGGAAATG ATCTGGCTCG ATGCCTAAGA TGGGGAGGAG GTTATGAAGG ACAGAATCTG 
GCAAAGATCC TCAAGGATTT AGAGATGAGT AAAGTGGTAC ATATGGATCG ATGGTCTGTG 
GAGGTGATAC CTCAACAAAC TGAAGAAAAA AGTGACCCAG TCCCCTTTCA AATCATCAAT 
AACTACTTCT CTATTGGCGT GGATGCCTCT ATTGCTCATC GATTCCACAT CATGCGAGAG 
AAATATCCGG AGAAGTTCAA CAGCAGAATG AAGAACAAGC TATGGTACTT CGAATTTGCC 
ACATCTGAAT CCATCTTCTC AACATGCAAA AAGCTGGAGG AGTCTTTGAC AGTTGAGATC 
TGTGGGAAAC CGCTGGATCT GAGCAACCTG TCCCTAGAAG GCATCGCAGT GCTAAACATC 
CCTAGCATGC ATGGTGGCTC CAACCTCTGG GGTGATACCA GGAGACCCCA TGGGGATATC 
TATGGGATCA ACCAGGCCTT AGGTGCTACA GCTAAAGTCA TCACCGACCC TGATATCCTG 
AAAACCTGTG TACCAGACCT AAGTGACAAG AGACTGGAAG TGGTTGGGCT GGAGGGTGCA 
ATTGAGATGG GCCAAATCTA TACCAAGCTC AAGAATGCTG GACGTCGGCT GGCCAAGTGC 
TCTGAGATCA CCTTCCACAC CACAAAAACC CTTCCCATGC AAATTGACGG AGAACCCTGG 
ATGCAGACGC CCTGTACAAT CAAGATCACC CACAAGAACC AGATGCCCAT GCTCATGGGC 
CCACCCCCCC GCTCCACCAA TTTCTTTGGC TTCTTGAGCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016874393.1
CDS	243..2804
Translation	MAKERGLISPSDFAQLQKYMEYSTKKVSDVLKLFEDGEMAKYVQGDAIGYEGFQQFLKIYLEVDNVPRHLSLALFQSFETGHCLNETNVTKDVVCLNDVSCYFSLLEGGRPEDKLEFTFKLYDTDRNGILDSSVSWGPCMLGKGICVNLSAPSCPNSSRVLRNRRGWGERLSPLGLNLKVSPKLLTPQRECWIHELGLPYKHTNTFIYLADIPENERCESGVEPRNTDFWKRVWMVVVKGIWLWLLPFLLQEVDKIILQMMRVAEYLDWDVSELRPILQEMMKEIDYDGSGSVSQAEWVRAGATTVPLLVLLGLEMTLKDDGQHMWRPKRFPRPVYCNLCESSIGLGKQGLSCNLCKYTVHDQCAMKALPCEVSTYAKSRKDIGVQSHVWVRGGCESGRCDRCQKKIRIYHSLTGLHCVWCHLEIHDDCLQAVGHECDCGLLRDHILPPSSIYPSVLASGPDRKNSKTSQKTMDDLNLSTSEALRIDPVPNTHPLLVFVNPKSGGKQGQRVLWKFQYILNPRQVFNLLKDGPEIGLRLFKDVPDSRILVCGGDGTVGWILETIDKANLPVLPPVAVLPLGTGNDLARCLRWGGGYEGQNLAKILKDLEMSKVVHMDRWSVEVIPQQTEEKSDPVPFQIINNYFSIGVDASIAHRFHIMREKYPEKFNSRMKNKLWYFEFATSESIFSTCKKLEESLTVEICGKPLDLSNLSLEGIAVLNIPSMHGGSNLWGDTRRPHGDIYGINQALGATAKVITDPDILKTCVPDLSDKRLEVVGLEGAIEMGQIYTKLKNAGRRLAKCSEITFHTTKTLPMQIDGEPWMQTPCTIKITHKNQMPMLMGPPPRSTNFFGFLS

Target ORF information:

RefSeq Version	XM_017018904.1
Organism	Homo sapiens(human)
Definition	Homo sapiens diacylglycerol kinase alpha (DGKA), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017018904.1

ORF Insert Sequence:

ATGGCCAAGG AGAGGGGCCT AATAAGCCCC AGTGATTTTG CCCAGCTGCA AAAATACATG 
GAATACTCCA CCAAAAAGGT CAGTGATGTC CTAAAGCTCT TCGAGGATGG CGAGATGGCT 
AAATATGTCC AAGGAGATGC CATTGGGTAC GAGGGATTCC AGCAATTCCT GAAAATCTAT 
CTCGAAGTGG ATAATGTTCC CAGACACCTA AGCCTGGCAC TGTTTCAATC CTTTGAGACT 
GGTCACTGCT TAAATGAGAC AAATGTGACA AAAGATGTGG TGTGTCTCAA TGATGTTTCC 
TGCTACTTTT CCCTTCTGGA GGGTGGTCGG CCAGAAGACA AGTTAGAATT CACCTTCAAG 
CTGTACGACA CGGACAGAAA TGGGATCCTG GACAGCTCAG TGAGTTGGGG ACCCTGTATG 
CTGGGCAAGG GAATATGTGT TAATTTGTCT GCACCCTCCT GCCCCAACTC TTCCAGGGTC 
TTAAGAAACA GAAGAGGATG GGGGGAGAGG TTGAGTCCTC TAGGACTAAA TTTGAAAGTA 
AGTCCCAAGC TCTTGACCCC TCAAAGAGAA TGCTGGATAC ATGAATTGGG TCTGCCTTAC 
AAACATACAA ACACATTTAT TTACTTGGCA GACATACCTG AGAATGAGAG GTGTGAATCT 
GGGGTGGAAC CCAGGAACAC TGATTTCTGG AAAAGAGTTT GGATGGTGGT AGTAAAGGGG 
ATATGGCTGT GGCTCTTGCC CTTTTTGCTC CAGGAAGTGG ACAAAATTAT CCTACAGATG 
ATGCGAGTGG CTGAATACCT GGATTGGGAT GTGTCTGAGC TGAGGCCGAT TCTTCAGGAG 
ATGATGAAAG AGATTGACTA TGATGGCAGT GGCTCTGTCT CTCAAGCTGA GTGGGTCCGG 
GCTGGGGCCA CCACCGTGCC ACTGCTAGTG CTGCTGGGTC TGGAGATGAC TCTGAAGGAC 
GACGGACAGC ACATGTGGAG GCCCAAGAGG TTCCCCAGAC CAGTCTACTG CAATCTGTGC 
GAGTCAAGCA TTGGTCTTGG CAAACAGGGA CTGAGCTGTA ACCTCTGTAA GTACACTGTT 
CACGACCAGT GTGCCATGAA AGCCCTGCCT TGTGAAGTCA GCACCTATGC CAAGTCTCGG 
AAGGACATTG GTGTCCAATC ACATGTGTGG GTGCGAGGAG GCTGTGAGTC CGGGCGCTGC 
GACCGCTGTC AGAAAAAGAT CCGGATCTAC CACAGTCTGA CCGGGCTGCA TTGTGTATGG 
TGCCACCTAG AGATCCACGA TGACTGCCTG CAAGCGGTGG GCCATGAGTG TGACTGTGGG 
CTGCTCCGGG ATCACATCCT GCCTCCATCT TCCATCTATC CCAGTGTCCT GGCCTCTGGA 
CCGGATCGTA AAAATAGCAA AACAAGCCAG AAGACCATGG ATGATTTAAA TTTGAGCACC 
TCTGAGGCTC TGCGGATTGA CCCTGTTCCT AACACCCACC CACTTCTCGT CTTTGTCAAT 
CCTAAGAGTG GCGGGAAGCA GGGGCAAAGG GTGCTCTGGA AGTTCCAGTA TATATTAAAC 
CCTCGACAGG TGTTCAACCT CCTAAAGGAT GGTCCTGAGA TAGGGCTCCG ATTATTCAAG 
GATGTTCCTG ATAGCCGGAT TTTGGTGTGT GGTGGAGACG GCACAGTAGG CTGGATTCTA 
GAGACCATTG ACAAAGCTAA CTTGCCAGTT TTGCCTCCTG TTGCTGTGTT GCCCCTGGGT 
ACTGGAAATG ATCTGGCTCG ATGCCTAAGA TGGGGAGGAG GTTATGAAGG ACAGAATCTG 
GCAAAGATCC TCAAGGATTT AGAGATGAGT AAAGTGGTAC ATATGGATCG ATGGTCTGTG 
GAGGTGATAC CTCAACAAAC TGAAGAAAAA AGTGACCCAG TCCCCTTTCA AATCATCAAT 
AACTACTTCT CTATTGGCGT GGATGCCTCT ATTGCTCATC GATTCCACAT CATGCGAGAG 
AAATATCCGG AGAAGTTCAA CAGCAGAATG AAGAACAAGC TATGGTACTT CGAATTTGCC 
ACATCTGAAT CCATCTTCTC AACATGCAAA AAGCTGGAGG AGTCTTTGAC AGTTGAGATC 
TGTGGGAAAC CGCTGGATCT GAGCAACCTG TCCCTAGAAG GCATCGCAGT GCTAAACATC 
CCTAGCATGC ATGGTGGCTC CAACCTCTGG GGTGATACCA GGAGACCCCA TGGGGATATC 
TATGGGATCA ACCAGGCCTT AGGTGCTACA GCTAAAGTCA TCACCGACCC TGATATCCTG 
AAAACCTGTG TACCAGACCT AAGTGACAAG AGACTGGAAG TGGTTGGGCT GGAGGGTGCA 
ATTGAGATGG GCCAAATCTA TACCAAGCTC AAGAATGCTG GACGTCGGCT GGCCAAGTGC 
TCTGAGATCA CCTTCCACAC CACAAAAACC CTTCCCATGC AAATTGACGG AGAACCCTGG 
ATGCAGACGC CCTGTACAAT CAAGATCACC CACAAGAACC AGATGCCCAT GCTCATGGGC 
CCACCCCCCC GCTCCACCAA TTTCTTTGGC TTCTTGAGCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DGKA ( XM_017018901.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016874390.1 Homo sapiens diacylglycerol kinase alpha (DGKA), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu83568
Clone ID Related Accession (Same CDS sequence)	XM_017018903.1 , XM_017018904.1 , XM_017018901.2 , XM_017018907.1 , XM_017018906.1 , XM_017018905.1 , XM_017018906.2 , XM_017018901.1 , XM_017018902.1
Accession Version	XM_017018901.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2562bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	diacylglycerol kinase alpha isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017018901.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAGG AGAGGGGCCT AATAAGCCCC AGTGATTTTG CCCAGCTGCA AAAATACATG 
GAATACTCCA CCAAAAAGGT CAGTGATGTC CTAAAGCTCT TCGAGGATGG CGAGATGGCT 
AAATATGTCC AAGGAGATGC CATTGGGTAC GAGGGATTCC AGCAATTCCT GAAAATCTAT 
CTCGAAGTGG ATAATGTTCC CAGACACCTA AGCCTGGCAC TGTTTCAATC CTTTGAGACT 
GGTCACTGCT TAAATGAGAC AAATGTGACA AAAGATGTGG TGTGTCTCAA TGATGTTTCC 
TGCTACTTTT CCCTTCTGGA GGGTGGTCGG CCAGAAGACA AGTTAGAATT CACCTTCAAG 
CTGTACGACA CGGACAGAAA TGGGATCCTG GACAGCTCAG TGAGTTGGGG ACCCTGTATG 
CTGGGCAAGG GAATATGTGT TAATTTGTCT GCACCCTCCT GCCCCAACTC TTCCAGGGTC 
TTAAGAAACA GAAGAGGATG GGGGGAGAGG TTGAGTCCTC TAGGACTAAA TTTGAAAGTA 
AGTCCCAAGC TCTTGACCCC TCAAAGAGAA TGCTGGATAC ATGAATTGGG TCTGCCTTAC 
AAACATACAA ACACATTTAT TTACTTGGCA GACATACCTG AGAATGAGAG GTGTGAATCT 
GGGGTGGAAC CCAGGAACAC TGATTTCTGG AAAAGAGTTT GGATGGTGGT AGTAAAGGGG 
ATATGGCTGT GGCTCTTGCC CTTTTTGCTC CAGGAAGTGG ACAAAATTAT CCTACAGATG 
ATGCGAGTGG CTGAATACCT GGATTGGGAT GTGTCTGAGC TGAGGCCGAT TCTTCAGGAG 
ATGATGAAAG AGATTGACTA TGATGGCAGT GGCTCTGTCT CTCAAGCTGA GTGGGTCCGG 
GCTGGGGCCA CCACCGTGCC ACTGCTAGTG CTGCTGGGTC TGGAGATGAC TCTGAAGGAC 
GACGGACAGC ACATGTGGAG GCCCAAGAGG TTCCCCAGAC CAGTCTACTG CAATCTGTGC 
GAGTCAAGCA TTGGTCTTGG CAAACAGGGA CTGAGCTGTA ACCTCTGTAA GTACACTGTT 
CACGACCAGT GTGCCATGAA AGCCCTGCCT TGTGAAGTCA GCACCTATGC CAAGTCTCGG 
AAGGACATTG GTGTCCAATC ACATGTGTGG GTGCGAGGAG GCTGTGAGTC CGGGCGCTGC 
GACCGCTGTC AGAAAAAGAT CCGGATCTAC CACAGTCTGA CCGGGCTGCA TTGTGTATGG 
TGCCACCTAG AGATCCACGA TGACTGCCTG CAAGCGGTGG GCCATGAGTG TGACTGTGGG 
CTGCTCCGGG ATCACATCCT GCCTCCATCT TCCATCTATC CCAGTGTCCT GGCCTCTGGA 
CCGGATCGTA AAAATAGCAA AACAAGCCAG AAGACCATGG ATGATTTAAA TTTGAGCACC 
TCTGAGGCTC TGCGGATTGA CCCTGTTCCT AACACCCACC CACTTCTCGT CTTTGTCAAT 
CCTAAGAGTG GCGGGAAGCA GGGGCAAAGG GTGCTCTGGA AGTTCCAGTA TATATTAAAC 
CCTCGACAGG TGTTCAACCT CCTAAAGGAT GGTCCTGAGA TAGGGCTCCG ATTATTCAAG 
GATGTTCCTG ATAGCCGGAT TTTGGTGTGT GGTGGAGACG GCACAGTAGG CTGGATTCTA 
GAGACCATTG ACAAAGCTAA CTTGCCAGTT TTGCCTCCTG TTGCTGTGTT GCCCCTGGGT 
ACTGGAAATG ATCTGGCTCG ATGCCTAAGA TGGGGAGGAG GTTATGAAGG ACAGAATCTG 
GCAAAGATCC TCAAGGATTT AGAGATGAGT AAAGTGGTAC ATATGGATCG ATGGTCTGTG 
GAGGTGATAC CTCAACAAAC TGAAGAAAAA AGTGACCCAG TCCCCTTTCA AATCATCAAT 
AACTACTTCT CTATTGGCGT GGATGCCTCT ATTGCTCATC GATTCCACAT CATGCGAGAG 
AAATATCCGG AGAAGTTCAA CAGCAGAATG AAGAACAAGC TATGGTACTT CGAATTTGCC 
ACATCTGAAT CCATCTTCTC AACATGCAAA AAGCTGGAGG AGTCTTTGAC AGTTGAGATC 
TGTGGGAAAC CGCTGGATCT GAGCAACCTG TCCCTAGAAG GCATCGCAGT GCTAAACATC 
CCTAGCATGC ATGGTGGCTC CAACCTCTGG GGTGATACCA GGAGACCCCA TGGGGATATC 
TATGGGATCA ACCAGGCCTT AGGTGCTACA GCTAAAGTCA TCACCGACCC TGATATCCTG 
AAAACCTGTG TACCAGACCT AAGTGACAAG AGACTGGAAG TGGTTGGGCT GGAGGGTGCA 
ATTGAGATGG GCCAAATCTA TACCAAGCTC AAGAATGCTG GACGTCGGCT GGCCAAGTGC 
TCTGAGATCA CCTTCCACAC CACAAAAACC CTTCCCATGC AAATTGACGG AGAACCCTGG 
ATGCAGACGC CCTGTACAAT CAAGATCACC CACAAGAACC AGATGCCCAT GCTCATGGGC 
CCACCCCCCC GCTCCACCAA TTTCTTTGGC TTCTTGAGCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016874390.1
CDS	336..2897
Translation	MAKERGLISPSDFAQLQKYMEYSTKKVSDVLKLFEDGEMAKYVQGDAIGYEGFQQFLKIYLEVDNVPRHLSLALFQSFETGHCLNETNVTKDVVCLNDVSCYFSLLEGGRPEDKLEFTFKLYDTDRNGILDSSVSWGPCMLGKGICVNLSAPSCPNSSRVLRNRRGWGERLSPLGLNLKVSPKLLTPQRECWIHELGLPYKHTNTFIYLADIPENERCESGVEPRNTDFWKRVWMVVVKGIWLWLLPFLLQEVDKIILQMMRVAEYLDWDVSELRPILQEMMKEIDYDGSGSVSQAEWVRAGATTVPLLVLLGLEMTLKDDGQHMWRPKRFPRPVYCNLCESSIGLGKQGLSCNLCKYTVHDQCAMKALPCEVSTYAKSRKDIGVQSHVWVRGGCESGRCDRCQKKIRIYHSLTGLHCVWCHLEIHDDCLQAVGHECDCGLLRDHILPPSSIYPSVLASGPDRKNSKTSQKTMDDLNLSTSEALRIDPVPNTHPLLVFVNPKSGGKQGQRVLWKFQYILNPRQVFNLLKDGPEIGLRLFKDVPDSRILVCGGDGTVGWILETIDKANLPVLPPVAVLPLGTGNDLARCLRWGGGYEGQNLAKILKDLEMSKVVHMDRWSVEVIPQQTEEKSDPVPFQIINNYFSIGVDASIAHRFHIMREKYPEKFNSRMKNKLWYFEFATSESIFSTCKKLEESLTVEICGKPLDLSNLSLEGIAVLNIPSMHGGSNLWGDTRRPHGDIYGINQALGATAKVITDPDILKTCVPDLSDKRLEVVGLEGAIEMGQIYTKLKNAGRRLAKCSEITFHTTKTLPMQIDGEPWMQTPCTIKITHKNQMPMLMGPPPRSTNFFGFLS

Target ORF information:

RefSeq Version	XM_017018901.2
Organism	Homo sapiens(human)
Definition	Homo sapiens diacylglycerol kinase alpha (DGKA), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017018901.2

ORF Insert Sequence:

ATGGCCAAGG AGAGGGGCCT AATAAGCCCC AGTGATTTTG CCCAGCTGCA AAAATACATG 
GAATACTCCA CCAAAAAGGT CAGTGATGTC CTAAAGCTCT TCGAGGATGG CGAGATGGCT 
AAATATGTCC AAGGAGATGC CATTGGGTAC GAGGGATTCC AGCAATTCCT GAAAATCTAT 
CTCGAAGTGG ATAATGTTCC CAGACACCTA AGCCTGGCAC TGTTTCAATC CTTTGAGACT 
GGTCACTGCT TAAATGAGAC AAATGTGACA AAAGATGTGG TGTGTCTCAA TGATGTTTCC 
TGCTACTTTT CCCTTCTGGA GGGTGGTCGG CCAGAAGACA AGTTAGAATT CACCTTCAAG 
CTGTACGACA CGGACAGAAA TGGGATCCTG GACAGCTCAG TGAGTTGGGG ACCCTGTATG 
CTGGGCAAGG GAATATGTGT TAATTTGTCT GCACCCTCCT GCCCCAACTC TTCCAGGGTC 
TTAAGAAACA GAAGAGGATG GGGGGAGAGG TTGAGTCCTC TAGGACTAAA TTTGAAAGTA 
AGTCCCAAGC TCTTGACCCC TCAAAGAGAA TGCTGGATAC ATGAATTGGG TCTGCCTTAC 
AAACATACAA ACACATTTAT TTACTTGGCA GACATACCTG AGAATGAGAG GTGTGAATCT 
GGGGTGGAAC CCAGGAACAC TGATTTCTGG AAAAGAGTTT GGATGGTGGT AGTAAAGGGG 
ATATGGCTGT GGCTCTTGCC CTTTTTGCTC CAGGAAGTGG ACAAAATTAT CCTACAGATG 
ATGCGAGTGG CTGAATACCT GGATTGGGAT GTGTCTGAGC TGAGGCCGAT TCTTCAGGAG 
ATGATGAAAG AGATTGACTA TGATGGCAGT GGCTCTGTCT CTCAAGCTGA GTGGGTCCGG 
GCTGGGGCCA CCACCGTGCC ACTGCTAGTG CTGCTGGGTC TGGAGATGAC TCTGAAGGAC 
GACGGACAGC ACATGTGGAG GCCCAAGAGG TTCCCCAGAC CAGTCTACTG CAATCTGTGC 
GAGTCAAGCA TTGGTCTTGG CAAACAGGGA CTGAGCTGTA ACCTCTGTAA GTACACTGTT 
CACGACCAGT GTGCCATGAA AGCCCTGCCT TGTGAAGTCA GCACCTATGC CAAGTCTCGG 
AAGGACATTG GTGTCCAATC ACATGTGTGG GTGCGAGGAG GCTGTGAGTC CGGGCGCTGC 
GACCGCTGTC AGAAAAAGAT CCGGATCTAC CACAGTCTGA CCGGGCTGCA TTGTGTATGG 
TGCCACCTAG AGATCCACGA TGACTGCCTG CAAGCGGTGG GCCATGAGTG TGACTGTGGG 
CTGCTCCGGG ATCACATCCT GCCTCCATCT TCCATCTATC CCAGTGTCCT GGCCTCTGGA 
CCGGATCGTA AAAATAGCAA AACAAGCCAG AAGACCATGG ATGATTTAAA TTTGAGCACC 
TCTGAGGCTC TGCGGATTGA CCCTGTTCCT AACACCCACC CACTTCTCGT CTTTGTCAAT 
CCTAAGAGTG GCGGGAAGCA GGGGCAAAGG GTGCTCTGGA AGTTCCAGTA TATATTAAAC 
CCTCGACAGG TGTTCAACCT CCTAAAGGAT GGTCCTGAGA TAGGGCTCCG ATTATTCAAG 
GATGTTCCTG ATAGCCGGAT TTTGGTGTGT GGTGGAGACG GCACAGTAGG CTGGATTCTA 
GAGACCATTG ACAAAGCTAA CTTGCCAGTT TTGCCTCCTG TTGCTGTGTT GCCCCTGGGT 
ACTGGAAATG ATCTGGCTCG ATGCCTAAGA TGGGGAGGAG GTTATGAAGG ACAGAATCTG 
GCAAAGATCC TCAAGGATTT AGAGATGAGT AAAGTGGTAC ATATGGATCG ATGGTCTGTG 
GAGGTGATAC CTCAACAAAC TGAAGAAAAA AGTGACCCAG TCCCCTTTCA AATCATCAAT 
AACTACTTCT CTATTGGCGT GGATGCCTCT ATTGCTCATC GATTCCACAT CATGCGAGAG 
AAATATCCGG AGAAGTTCAA CAGCAGAATG AAGAACAAGC TATGGTACTT CGAATTTGCC 
ACATCTGAAT CCATCTTCTC AACATGCAAA AAGCTGGAGG AGTCTTTGAC AGTTGAGATC 
TGTGGGAAAC CGCTGGATCT GAGCAACCTG TCCCTAGAAG GCATCGCAGT GCTAAACATC 
CCTAGCATGC ATGGTGGCTC CAACCTCTGG GGTGATACCA GGAGACCCCA TGGGGATATC 
TATGGGATCA ACCAGGCCTT AGGTGCTACA GCTAAAGTCA TCACCGACCC TGATATCCTG 
AAAACCTGTG TACCAGACCT AAGTGACAAG AGACTGGAAG TGGTTGGGCT GGAGGGTGCA 
ATTGAGATGG GCCAAATCTA TACCAAGCTC AAGAATGCTG GACGTCGGCT GGCCAAGTGC 
TCTGAGATCA CCTTCCACAC CACAAAAACC CTTCCCATGC AAATTGACGG AGAACCCTGG 
ATGCAGACGC CCTGTACAAT CAAGATCACC CACAAGAACC AGATGCCCAT GCTCATGGGC 
CCACCCCCCC GCTCCACCAA TTTCTTTGGC TTCTTGAGCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DGKA ( XM_017018907.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016874396.1 Homo sapiens diacylglycerol kinase alpha (DGKA), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OHu83568
Clone ID Related Accession (Same CDS sequence)	XM_017018903.1 , XM_017018904.1 , XM_017018901.2 , XM_017018907.1 , XM_017018906.1 , XM_017018905.1 , XM_017018906.2 , XM_017018901.1 , XM_017018902.1
Accession Version	XM_017018907.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2562bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	diacylglycerol kinase alpha isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAGG AGAGGGGCCT AATAAGCCCC AGTGATTTTG CCCAGCTGCA AAAATACATG 
GAATACTCCA CCAAAAAGGT CAGTGATGTC CTAAAGCTCT TCGAGGATGG CGAGATGGCT 
AAATATGTCC AAGGAGATGC CATTGGGTAC GAGGGATTCC AGCAATTCCT GAAAATCTAT 
CTCGAAGTGG ATAATGTTCC CAGACACCTA AGCCTGGCAC TGTTTCAATC CTTTGAGACT 
GGTCACTGCT TAAATGAGAC AAATGTGACA AAAGATGTGG TGTGTCTCAA TGATGTTTCC 
TGCTACTTTT CCCTTCTGGA GGGTGGTCGG CCAGAAGACA AGTTAGAATT CACCTTCAAG 
CTGTACGACA CGGACAGAAA TGGGATCCTG GACAGCTCAG TGAGTTGGGG ACCCTGTATG 
CTGGGCAAGG GAATATGTGT TAATTTGTCT GCACCCTCCT GCCCCAACTC TTCCAGGGTC 
TTAAGAAACA GAAGAGGATG GGGGGAGAGG TTGAGTCCTC TAGGACTAAA TTTGAAAGTA 
AGTCCCAAGC TCTTGACCCC TCAAAGAGAA TGCTGGATAC ATGAATTGGG TCTGCCTTAC 
AAACATACAA ACACATTTAT TTACTTGGCA GACATACCTG AGAATGAGAG GTGTGAATCT 
GGGGTGGAAC CCAGGAACAC TGATTTCTGG AAAAGAGTTT GGATGGTGGT AGTAAAGGGG 
ATATGGCTGT GGCTCTTGCC CTTTTTGCTC CAGGAAGTGG ACAAAATTAT CCTACAGATG 
ATGCGAGTGG CTGAATACCT GGATTGGGAT GTGTCTGAGC TGAGGCCGAT TCTTCAGGAG 
ATGATGAAAG AGATTGACTA TGATGGCAGT GGCTCTGTCT CTCAAGCTGA GTGGGTCCGG 
GCTGGGGCCA CCACCGTGCC ACTGCTAGTG CTGCTGGGTC TGGAGATGAC TCTGAAGGAC 
GACGGACAGC ACATGTGGAG GCCCAAGAGG TTCCCCAGAC CAGTCTACTG CAATCTGTGC 
GAGTCAAGCA TTGGTCTTGG CAAACAGGGA CTGAGCTGTA ACCTCTGTAA GTACACTGTT 
CACGACCAGT GTGCCATGAA AGCCCTGCCT TGTGAAGTCA GCACCTATGC CAAGTCTCGG 
AAGGACATTG GTGTCCAATC ACATGTGTGG GTGCGAGGAG GCTGTGAGTC CGGGCGCTGC 
GACCGCTGTC AGAAAAAGAT CCGGATCTAC CACAGTCTGA CCGGGCTGCA TTGTGTATGG 
TGCCACCTAG AGATCCACGA TGACTGCCTG CAAGCGGTGG GCCATGAGTG TGACTGTGGG 
CTGCTCCGGG ATCACATCCT GCCTCCATCT TCCATCTATC CCAGTGTCCT GGCCTCTGGA 
CCGGATCGTA AAAATAGCAA AACAAGCCAG AAGACCATGG ATGATTTAAA TTTGAGCACC 
TCTGAGGCTC TGCGGATTGA CCCTGTTCCT AACACCCACC CACTTCTCGT CTTTGTCAAT 
CCTAAGAGTG GCGGGAAGCA GGGGCAAAGG GTGCTCTGGA AGTTCCAGTA TATATTAAAC 
CCTCGACAGG TGTTCAACCT CCTAAAGGAT GGTCCTGAGA TAGGGCTCCG ATTATTCAAG 
GATGTTCCTG ATAGCCGGAT TTTGGTGTGT GGTGGAGACG GCACAGTAGG CTGGATTCTA 
GAGACCATTG ACAAAGCTAA CTTGCCAGTT TTGCCTCCTG TTGCTGTGTT GCCCCTGGGT 
ACTGGAAATG ATCTGGCTCG ATGCCTAAGA TGGGGAGGAG GTTATGAAGG ACAGAATCTG 
GCAAAGATCC TCAAGGATTT AGAGATGAGT AAAGTGGTAC ATATGGATCG ATGGTCTGTG 
GAGGTGATAC CTCAACAAAC TGAAGAAAAA AGTGACCCAG TCCCCTTTCA AATCATCAAT 
AACTACTTCT CTATTGGCGT GGATGCCTCT ATTGCTCATC GATTCCACAT CATGCGAGAG 
AAATATCCGG AGAAGTTCAA CAGCAGAATG AAGAACAAGC TATGGTACTT CGAATTTGCC 
ACATCTGAAT CCATCTTCTC AACATGCAAA AAGCTGGAGG AGTCTTTGAC AGTTGAGATC 
TGTGGGAAAC CGCTGGATCT GAGCAACCTG TCCCTAGAAG GCATCGCAGT GCTAAACATC 
CCTAGCATGC ATGGTGGCTC CAACCTCTGG GGTGATACCA GGAGACCCCA TGGGGATATC 
TATGGGATCA ACCAGGCCTT AGGTGCTACA GCTAAAGTCA TCACCGACCC TGATATCCTG 
AAAACCTGTG TACCAGACCT AAGTGACAAG AGACTGGAAG TGGTTGGGCT GGAGGGTGCA 
ATTGAGATGG GCCAAATCTA TACCAAGCTC AAGAATGCTG GACGTCGGCT GGCCAAGTGC 
TCTGAGATCA CCTTCCACAC CACAAAAACC CTTCCCATGC AAATTGACGG AGAACCCTGG 
ATGCAGACGC CCTGTACAAT CAAGATCACC CACAAGAACC AGATGCCCAT GCTCATGGGC 
CCACCCCCCC GCTCCACCAA TTTCTTTGGC TTCTTGAGCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016874396.1
CDS	107..2668
Translation	MAKERGLISPSDFAQLQKYMEYSTKKVSDVLKLFEDGEMAKYVQGDAIGYEGFQQFLKIYLEVDNVPRHLSLALFQSFETGHCLNETNVTKDVVCLNDVSCYFSLLEGGRPEDKLEFTFKLYDTDRNGILDSSVSWGPCMLGKGICVNLSAPSCPNSSRVLRNRRGWGERLSPLGLNLKVSPKLLTPQRECWIHELGLPYKHTNTFIYLADIPENERCESGVEPRNTDFWKRVWMVVVKGIWLWLLPFLLQEVDKIILQMMRVAEYLDWDVSELRPILQEMMKEIDYDGSGSVSQAEWVRAGATTVPLLVLLGLEMTLKDDGQHMWRPKRFPRPVYCNLCESSIGLGKQGLSCNLCKYTVHDQCAMKALPCEVSTYAKSRKDIGVQSHVWVRGGCESGRCDRCQKKIRIYHSLTGLHCVWCHLEIHDDCLQAVGHECDCGLLRDHILPPSSIYPSVLASGPDRKNSKTSQKTMDDLNLSTSEALRIDPVPNTHPLLVFVNPKSGGKQGQRVLWKFQYILNPRQVFNLLKDGPEIGLRLFKDVPDSRILVCGGDGTVGWILETIDKANLPVLPPVAVLPLGTGNDLARCLRWGGGYEGQNLAKILKDLEMSKVVHMDRWSVEVIPQQTEEKSDPVPFQIINNYFSIGVDASIAHRFHIMREKYPEKFNSRMKNKLWYFEFATSESIFSTCKKLEESLTVEICGKPLDLSNLSLEGIAVLNIPSMHGGSNLWGDTRRPHGDIYGINQALGATAKVITDPDILKTCVPDLSDKRLEVVGLEGAIEMGQIYTKLKNAGRRLAKCSEITFHTTKTLPMQIDGEPWMQTPCTIKITHKNQMPMLMGPPPRSTNFFGFLS

Target ORF information:

RefSeq Version	XM_017018907.1
Organism	Homo sapiens(human)
Definition	Homo sapiens diacylglycerol kinase alpha (DGKA), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017018907.1

ORF Insert Sequence:

ATGGCCAAGG AGAGGGGCCT AATAAGCCCC AGTGATTTTG CCCAGCTGCA AAAATACATG 
GAATACTCCA CCAAAAAGGT CAGTGATGTC CTAAAGCTCT TCGAGGATGG CGAGATGGCT 
AAATATGTCC AAGGAGATGC CATTGGGTAC GAGGGATTCC AGCAATTCCT GAAAATCTAT 
CTCGAAGTGG ATAATGTTCC CAGACACCTA AGCCTGGCAC TGTTTCAATC CTTTGAGACT 
GGTCACTGCT TAAATGAGAC AAATGTGACA AAAGATGTGG TGTGTCTCAA TGATGTTTCC 
TGCTACTTTT CCCTTCTGGA GGGTGGTCGG CCAGAAGACA AGTTAGAATT CACCTTCAAG 
CTGTACGACA CGGACAGAAA TGGGATCCTG GACAGCTCAG TGAGTTGGGG ACCCTGTATG 
CTGGGCAAGG GAATATGTGT TAATTTGTCT GCACCCTCCT GCCCCAACTC TTCCAGGGTC 
TTAAGAAACA GAAGAGGATG GGGGGAGAGG TTGAGTCCTC TAGGACTAAA TTTGAAAGTA 
AGTCCCAAGC TCTTGACCCC TCAAAGAGAA TGCTGGATAC ATGAATTGGG TCTGCCTTAC 
AAACATACAA ACACATTTAT TTACTTGGCA GACATACCTG AGAATGAGAG GTGTGAATCT 
GGGGTGGAAC CCAGGAACAC TGATTTCTGG AAAAGAGTTT GGATGGTGGT AGTAAAGGGG 
ATATGGCTGT GGCTCTTGCC CTTTTTGCTC CAGGAAGTGG ACAAAATTAT CCTACAGATG 
ATGCGAGTGG CTGAATACCT GGATTGGGAT GTGTCTGAGC TGAGGCCGAT TCTTCAGGAG 
ATGATGAAAG AGATTGACTA TGATGGCAGT GGCTCTGTCT CTCAAGCTGA GTGGGTCCGG 
GCTGGGGCCA CCACCGTGCC ACTGCTAGTG CTGCTGGGTC TGGAGATGAC TCTGAAGGAC 
GACGGACAGC ACATGTGGAG GCCCAAGAGG TTCCCCAGAC CAGTCTACTG CAATCTGTGC 
GAGTCAAGCA TTGGTCTTGG CAAACAGGGA CTGAGCTGTA ACCTCTGTAA GTACACTGTT 
CACGACCAGT GTGCCATGAA AGCCCTGCCT TGTGAAGTCA GCACCTATGC CAAGTCTCGG 
AAGGACATTG GTGTCCAATC ACATGTGTGG GTGCGAGGAG GCTGTGAGTC CGGGCGCTGC 
GACCGCTGTC AGAAAAAGAT CCGGATCTAC CACAGTCTGA CCGGGCTGCA TTGTGTATGG 
TGCCACCTAG AGATCCACGA TGACTGCCTG CAAGCGGTGG GCCATGAGTG TGACTGTGGG 
CTGCTCCGGG ATCACATCCT GCCTCCATCT TCCATCTATC CCAGTGTCCT GGCCTCTGGA 
CCGGATCGTA AAAATAGCAA AACAAGCCAG AAGACCATGG ATGATTTAAA TTTGAGCACC 
TCTGAGGCTC TGCGGATTGA CCCTGTTCCT AACACCCACC CACTTCTCGT CTTTGTCAAT 
CCTAAGAGTG GCGGGAAGCA GGGGCAAAGG GTGCTCTGGA AGTTCCAGTA TATATTAAAC 
CCTCGACAGG TGTTCAACCT CCTAAAGGAT GGTCCTGAGA TAGGGCTCCG ATTATTCAAG 
GATGTTCCTG ATAGCCGGAT TTTGGTGTGT GGTGGAGACG GCACAGTAGG CTGGATTCTA 
GAGACCATTG ACAAAGCTAA CTTGCCAGTT TTGCCTCCTG TTGCTGTGTT GCCCCTGGGT 
ACTGGAAATG ATCTGGCTCG ATGCCTAAGA TGGGGAGGAG GTTATGAAGG ACAGAATCTG 
GCAAAGATCC TCAAGGATTT AGAGATGAGT AAAGTGGTAC ATATGGATCG ATGGTCTGTG 
GAGGTGATAC CTCAACAAAC TGAAGAAAAA AGTGACCCAG TCCCCTTTCA AATCATCAAT 
AACTACTTCT CTATTGGCGT GGATGCCTCT ATTGCTCATC GATTCCACAT CATGCGAGAG 
AAATATCCGG AGAAGTTCAA CAGCAGAATG AAGAACAAGC TATGGTACTT CGAATTTGCC 
ACATCTGAAT CCATCTTCTC AACATGCAAA AAGCTGGAGG AGTCTTTGAC AGTTGAGATC 
TGTGGGAAAC CGCTGGATCT GAGCAACCTG TCCCTAGAAG GCATCGCAGT GCTAAACATC 
CCTAGCATGC ATGGTGGCTC CAACCTCTGG GGTGATACCA GGAGACCCCA TGGGGATATC 
TATGGGATCA ACCAGGCCTT AGGTGCTACA GCTAAAGTCA TCACCGACCC TGATATCCTG 
AAAACCTGTG TACCAGACCT AAGTGACAAG AGACTGGAAG TGGTTGGGCT GGAGGGTGCA 
ATTGAGATGG GCCAAATCTA TACCAAGCTC AAGAATGCTG GACGTCGGCT GGCCAAGTGC 
TCTGAGATCA CCTTCCACAC CACAAAAACC CTTCCCATGC AAATTGACGG AGAACCCTGG 
ATGCAGACGC CCTGTACAAT CAAGATCACC CACAAGAACC AGATGCCCAT GCTCATGGGC 
CCACCCCCCC GCTCCACCAA TTTCTTTGGC TTCTTGAGCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DGKA ( XM_017018906.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016874395.1 Homo sapiens diacylglycerol kinase alpha (DGKA), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OHu83568
Clone ID Related Accession (Same CDS sequence)	XM_017018903.1 , XM_017018904.1 , XM_017018901.2 , XM_017018907.1 , XM_017018906.1 , XM_017018905.1 , XM_017018906.2 , XM_017018901.1 , XM_017018902.1
Accession Version	XM_017018906.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2562bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	diacylglycerol kinase alpha isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAGG AGAGGGGCCT AATAAGCCCC AGTGATTTTG CCCAGCTGCA AAAATACATG 
GAATACTCCA CCAAAAAGGT CAGTGATGTC CTAAAGCTCT TCGAGGATGG CGAGATGGCT 
AAATATGTCC AAGGAGATGC CATTGGGTAC GAGGGATTCC AGCAATTCCT GAAAATCTAT 
CTCGAAGTGG ATAATGTTCC CAGACACCTA AGCCTGGCAC TGTTTCAATC CTTTGAGACT 
GGTCACTGCT TAAATGAGAC AAATGTGACA AAAGATGTGG TGTGTCTCAA TGATGTTTCC 
TGCTACTTTT CCCTTCTGGA GGGTGGTCGG CCAGAAGACA AGTTAGAATT CACCTTCAAG 
CTGTACGACA CGGACAGAAA TGGGATCCTG GACAGCTCAG TGAGTTGGGG ACCCTGTATG 
CTGGGCAAGG GAATATGTGT TAATTTGTCT GCACCCTCCT GCCCCAACTC TTCCAGGGTC 
TTAAGAAACA GAAGAGGATG GGGGGAGAGG TTGAGTCCTC TAGGACTAAA TTTGAAAGTA 
AGTCCCAAGC TCTTGACCCC TCAAAGAGAA TGCTGGATAC ATGAATTGGG TCTGCCTTAC 
AAACATACAA ACACATTTAT TTACTTGGCA GACATACCTG AGAATGAGAG GTGTGAATCT 
GGGGTGGAAC CCAGGAACAC TGATTTCTGG AAAAGAGTTT GGATGGTGGT AGTAAAGGGG 
ATATGGCTGT GGCTCTTGCC CTTTTTGCTC CAGGAAGTGG ACAAAATTAT CCTACAGATG 
ATGCGAGTGG CTGAATACCT GGATTGGGAT GTGTCTGAGC TGAGGCCGAT TCTTCAGGAG 
ATGATGAAAG AGATTGACTA TGATGGCAGT GGCTCTGTCT CTCAAGCTGA GTGGGTCCGG 
GCTGGGGCCA CCACCGTGCC ACTGCTAGTG CTGCTGGGTC TGGAGATGAC TCTGAAGGAC 
GACGGACAGC ACATGTGGAG GCCCAAGAGG TTCCCCAGAC CAGTCTACTG CAATCTGTGC 
GAGTCAAGCA TTGGTCTTGG CAAACAGGGA CTGAGCTGTA ACCTCTGTAA GTACACTGTT 
CACGACCAGT GTGCCATGAA AGCCCTGCCT TGTGAAGTCA GCACCTATGC CAAGTCTCGG 
AAGGACATTG GTGTCCAATC ACATGTGTGG GTGCGAGGAG GCTGTGAGTC CGGGCGCTGC 
GACCGCTGTC AGAAAAAGAT CCGGATCTAC CACAGTCTGA CCGGGCTGCA TTGTGTATGG 
TGCCACCTAG AGATCCACGA TGACTGCCTG CAAGCGGTGG GCCATGAGTG TGACTGTGGG 
CTGCTCCGGG ATCACATCCT GCCTCCATCT TCCATCTATC CCAGTGTCCT GGCCTCTGGA 
CCGGATCGTA AAAATAGCAA AACAAGCCAG AAGACCATGG ATGATTTAAA TTTGAGCACC 
TCTGAGGCTC TGCGGATTGA CCCTGTTCCT AACACCCACC CACTTCTCGT CTTTGTCAAT 
CCTAAGAGTG GCGGGAAGCA GGGGCAAAGG GTGCTCTGGA AGTTCCAGTA TATATTAAAC 
CCTCGACAGG TGTTCAACCT CCTAAAGGAT GGTCCTGAGA TAGGGCTCCG ATTATTCAAG 
GATGTTCCTG ATAGCCGGAT TTTGGTGTGT GGTGGAGACG GCACAGTAGG CTGGATTCTA 
GAGACCATTG ACAAAGCTAA CTTGCCAGTT TTGCCTCCTG TTGCTGTGTT GCCCCTGGGT 
ACTGGAAATG ATCTGGCTCG ATGCCTAAGA TGGGGAGGAG GTTATGAAGG ACAGAATCTG 
GCAAAGATCC TCAAGGATTT AGAGATGAGT AAAGTGGTAC ATATGGATCG ATGGTCTGTG 
GAGGTGATAC CTCAACAAAC TGAAGAAAAA AGTGACCCAG TCCCCTTTCA AATCATCAAT 
AACTACTTCT CTATTGGCGT GGATGCCTCT ATTGCTCATC GATTCCACAT CATGCGAGAG 
AAATATCCGG AGAAGTTCAA CAGCAGAATG AAGAACAAGC TATGGTACTT CGAATTTGCC 
ACATCTGAAT CCATCTTCTC AACATGCAAA AAGCTGGAGG AGTCTTTGAC AGTTGAGATC 
TGTGGGAAAC CGCTGGATCT GAGCAACCTG TCCCTAGAAG GCATCGCAGT GCTAAACATC 
CCTAGCATGC ATGGTGGCTC CAACCTCTGG GGTGATACCA GGAGACCCCA TGGGGATATC 
TATGGGATCA ACCAGGCCTT AGGTGCTACA GCTAAAGTCA TCACCGACCC TGATATCCTG 
AAAACCTGTG TACCAGACCT AAGTGACAAG AGACTGGAAG TGGTTGGGCT GGAGGGTGCA 
ATTGAGATGG GCCAAATCTA TACCAAGCTC AAGAATGCTG GACGTCGGCT GGCCAAGTGC 
TCTGAGATCA CCTTCCACAC CACAAAAACC CTTCCCATGC AAATTGACGG AGAACCCTGG 
ATGCAGACGC CCTGTACAAT CAAGATCACC CACAAGAACC AGATGCCCAT GCTCATGGGC 
CCACCCCCCC GCTCCACCAA TTTCTTTGGC TTCTTGAGCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016874395.1
CDS	284..2845
Translation	MAKERGLISPSDFAQLQKYMEYSTKKVSDVLKLFEDGEMAKYVQGDAIGYEGFQQFLKIYLEVDNVPRHLSLALFQSFETGHCLNETNVTKDVVCLNDVSCYFSLLEGGRPEDKLEFTFKLYDTDRNGILDSSVSWGPCMLGKGICVNLSAPSCPNSSRVLRNRRGWGERLSPLGLNLKVSPKLLTPQRECWIHELGLPYKHTNTFIYLADIPENERCESGVEPRNTDFWKRVWMVVVKGIWLWLLPFLLQEVDKIILQMMRVAEYLDWDVSELRPILQEMMKEIDYDGSGSVSQAEWVRAGATTVPLLVLLGLEMTLKDDGQHMWRPKRFPRPVYCNLCESSIGLGKQGLSCNLCKYTVHDQCAMKALPCEVSTYAKSRKDIGVQSHVWVRGGCESGRCDRCQKKIRIYHSLTGLHCVWCHLEIHDDCLQAVGHECDCGLLRDHILPPSSIYPSVLASGPDRKNSKTSQKTMDDLNLSTSEALRIDPVPNTHPLLVFVNPKSGGKQGQRVLWKFQYILNPRQVFNLLKDGPEIGLRLFKDVPDSRILVCGGDGTVGWILETIDKANLPVLPPVAVLPLGTGNDLARCLRWGGGYEGQNLAKILKDLEMSKVVHMDRWSVEVIPQQTEEKSDPVPFQIINNYFSIGVDASIAHRFHIMREKYPEKFNSRMKNKLWYFEFATSESIFSTCKKLEESLTVEICGKPLDLSNLSLEGIAVLNIPSMHGGSNLWGDTRRPHGDIYGINQALGATAKVITDPDILKTCVPDLSDKRLEVVGLEGAIEMGQIYTKLKNAGRRLAKCSEITFHTTKTLPMQIDGEPWMQTPCTIKITHKNQMPMLMGPPPRSTNFFGFLS

Target ORF information:

RefSeq Version	XM_017018906.1
Organism	Homo sapiens(human)
Definition	Homo sapiens diacylglycerol kinase alpha (DGKA), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017018906.1

ORF Insert Sequence:

ATGGCCAAGG AGAGGGGCCT AATAAGCCCC AGTGATTTTG CCCAGCTGCA AAAATACATG 
GAATACTCCA CCAAAAAGGT CAGTGATGTC CTAAAGCTCT TCGAGGATGG CGAGATGGCT 
AAATATGTCC AAGGAGATGC CATTGGGTAC GAGGGATTCC AGCAATTCCT GAAAATCTAT 
CTCGAAGTGG ATAATGTTCC CAGACACCTA AGCCTGGCAC TGTTTCAATC CTTTGAGACT 
GGTCACTGCT TAAATGAGAC AAATGTGACA AAAGATGTGG TGTGTCTCAA TGATGTTTCC 
TGCTACTTTT CCCTTCTGGA GGGTGGTCGG CCAGAAGACA AGTTAGAATT CACCTTCAAG 
CTGTACGACA CGGACAGAAA TGGGATCCTG GACAGCTCAG TGAGTTGGGG ACCCTGTATG 
CTGGGCAAGG GAATATGTGT TAATTTGTCT GCACCCTCCT GCCCCAACTC TTCCAGGGTC 
TTAAGAAACA GAAGAGGATG GGGGGAGAGG TTGAGTCCTC TAGGACTAAA TTTGAAAGTA 
AGTCCCAAGC TCTTGACCCC TCAAAGAGAA TGCTGGATAC ATGAATTGGG TCTGCCTTAC 
AAACATACAA ACACATTTAT TTACTTGGCA GACATACCTG AGAATGAGAG GTGTGAATCT 
GGGGTGGAAC CCAGGAACAC TGATTTCTGG AAAAGAGTTT GGATGGTGGT AGTAAAGGGG 
ATATGGCTGT GGCTCTTGCC CTTTTTGCTC CAGGAAGTGG ACAAAATTAT CCTACAGATG 
ATGCGAGTGG CTGAATACCT GGATTGGGAT GTGTCTGAGC TGAGGCCGAT TCTTCAGGAG 
ATGATGAAAG AGATTGACTA TGATGGCAGT GGCTCTGTCT CTCAAGCTGA GTGGGTCCGG 
GCTGGGGCCA CCACCGTGCC ACTGCTAGTG CTGCTGGGTC TGGAGATGAC TCTGAAGGAC 
GACGGACAGC ACATGTGGAG GCCCAAGAGG TTCCCCAGAC CAGTCTACTG CAATCTGTGC 
GAGTCAAGCA TTGGTCTTGG CAAACAGGGA CTGAGCTGTA ACCTCTGTAA GTACACTGTT 
CACGACCAGT GTGCCATGAA AGCCCTGCCT TGTGAAGTCA GCACCTATGC CAAGTCTCGG 
AAGGACATTG GTGTCCAATC ACATGTGTGG GTGCGAGGAG GCTGTGAGTC CGGGCGCTGC 
GACCGCTGTC AGAAAAAGAT CCGGATCTAC CACAGTCTGA CCGGGCTGCA TTGTGTATGG 
TGCCACCTAG AGATCCACGA TGACTGCCTG CAAGCGGTGG GCCATGAGTG TGACTGTGGG 
CTGCTCCGGG ATCACATCCT GCCTCCATCT TCCATCTATC CCAGTGTCCT GGCCTCTGGA 
CCGGATCGTA AAAATAGCAA AACAAGCCAG AAGACCATGG ATGATTTAAA TTTGAGCACC 
TCTGAGGCTC TGCGGATTGA CCCTGTTCCT AACACCCACC CACTTCTCGT CTTTGTCAAT 
CCTAAGAGTG GCGGGAAGCA GGGGCAAAGG GTGCTCTGGA AGTTCCAGTA TATATTAAAC 
CCTCGACAGG TGTTCAACCT CCTAAAGGAT GGTCCTGAGA TAGGGCTCCG ATTATTCAAG 
GATGTTCCTG ATAGCCGGAT TTTGGTGTGT GGTGGAGACG GCACAGTAGG CTGGATTCTA 
GAGACCATTG ACAAAGCTAA CTTGCCAGTT TTGCCTCCTG TTGCTGTGTT GCCCCTGGGT 
ACTGGAAATG ATCTGGCTCG ATGCCTAAGA TGGGGAGGAG GTTATGAAGG ACAGAATCTG 
GCAAAGATCC TCAAGGATTT AGAGATGAGT AAAGTGGTAC ATATGGATCG ATGGTCTGTG 
GAGGTGATAC CTCAACAAAC TGAAGAAAAA AGTGACCCAG TCCCCTTTCA AATCATCAAT 
AACTACTTCT CTATTGGCGT GGATGCCTCT ATTGCTCATC GATTCCACAT CATGCGAGAG 
AAATATCCGG AGAAGTTCAA CAGCAGAATG AAGAACAAGC TATGGTACTT CGAATTTGCC 
ACATCTGAAT CCATCTTCTC AACATGCAAA AAGCTGGAGG AGTCTTTGAC AGTTGAGATC 
TGTGGGAAAC CGCTGGATCT GAGCAACCTG TCCCTAGAAG GCATCGCAGT GCTAAACATC 
CCTAGCATGC ATGGTGGCTC CAACCTCTGG GGTGATACCA GGAGACCCCA TGGGGATATC 
TATGGGATCA ACCAGGCCTT AGGTGCTACA GCTAAAGTCA TCACCGACCC TGATATCCTG 
AAAACCTGTG TACCAGACCT AAGTGACAAG AGACTGGAAG TGGTTGGGCT GGAGGGTGCA 
ATTGAGATGG GCCAAATCTA TACCAAGCTC AAGAATGCTG GACGTCGGCT GGCCAAGTGC 
TCTGAGATCA CCTTCCACAC CACAAAAACC CTTCCCATGC AAATTGACGG AGAACCCTGG 
ATGCAGACGC CCTGTACAAT CAAGATCACC CACAAGAACC AGATGCCCAT GCTCATGGGC 
CCACCCCCCC GCTCCACCAA TTTCTTTGGC TTCTTGAGCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DGKA ( XM_017018905.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016874394.1 Homo sapiens diacylglycerol kinase alpha (DGKA), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OHu83568
Clone ID Related Accession (Same CDS sequence)	XM_017018903.1 , XM_017018904.1 , XM_017018901.2 , XM_017018907.1 , XM_017018906.1 , XM_017018905.1 , XM_017018906.2 , XM_017018901.1 , XM_017018902.1
Accession Version	XM_017018905.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2562bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	diacylglycerol kinase alpha isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAGG AGAGGGGCCT AATAAGCCCC AGTGATTTTG CCCAGCTGCA AAAATACATG 
GAATACTCCA CCAAAAAGGT CAGTGATGTC CTAAAGCTCT TCGAGGATGG CGAGATGGCT 
AAATATGTCC AAGGAGATGC CATTGGGTAC GAGGGATTCC AGCAATTCCT GAAAATCTAT 
CTCGAAGTGG ATAATGTTCC CAGACACCTA AGCCTGGCAC TGTTTCAATC CTTTGAGACT 
GGTCACTGCT TAAATGAGAC AAATGTGACA AAAGATGTGG TGTGTCTCAA TGATGTTTCC 
TGCTACTTTT CCCTTCTGGA GGGTGGTCGG CCAGAAGACA AGTTAGAATT CACCTTCAAG 
CTGTACGACA CGGACAGAAA TGGGATCCTG GACAGCTCAG TGAGTTGGGG ACCCTGTATG 
CTGGGCAAGG GAATATGTGT TAATTTGTCT GCACCCTCCT GCCCCAACTC TTCCAGGGTC 
TTAAGAAACA GAAGAGGATG GGGGGAGAGG TTGAGTCCTC TAGGACTAAA TTTGAAAGTA 
AGTCCCAAGC TCTTGACCCC TCAAAGAGAA TGCTGGATAC ATGAATTGGG TCTGCCTTAC 
AAACATACAA ACACATTTAT TTACTTGGCA GACATACCTG AGAATGAGAG GTGTGAATCT 
GGGGTGGAAC CCAGGAACAC TGATTTCTGG AAAAGAGTTT GGATGGTGGT AGTAAAGGGG 
ATATGGCTGT GGCTCTTGCC CTTTTTGCTC CAGGAAGTGG ACAAAATTAT CCTACAGATG 
ATGCGAGTGG CTGAATACCT GGATTGGGAT GTGTCTGAGC TGAGGCCGAT TCTTCAGGAG 
ATGATGAAAG AGATTGACTA TGATGGCAGT GGCTCTGTCT CTCAAGCTGA GTGGGTCCGG 
GCTGGGGCCA CCACCGTGCC ACTGCTAGTG CTGCTGGGTC TGGAGATGAC TCTGAAGGAC 
GACGGACAGC ACATGTGGAG GCCCAAGAGG TTCCCCAGAC CAGTCTACTG CAATCTGTGC 
GAGTCAAGCA TTGGTCTTGG CAAACAGGGA CTGAGCTGTA ACCTCTGTAA GTACACTGTT 
CACGACCAGT GTGCCATGAA AGCCCTGCCT TGTGAAGTCA GCACCTATGC CAAGTCTCGG 
AAGGACATTG GTGTCCAATC ACATGTGTGG GTGCGAGGAG GCTGTGAGTC CGGGCGCTGC 
GACCGCTGTC AGAAAAAGAT CCGGATCTAC CACAGTCTGA CCGGGCTGCA TTGTGTATGG 
TGCCACCTAG AGATCCACGA TGACTGCCTG CAAGCGGTGG GCCATGAGTG TGACTGTGGG 
CTGCTCCGGG ATCACATCCT GCCTCCATCT TCCATCTATC CCAGTGTCCT GGCCTCTGGA 
CCGGATCGTA AAAATAGCAA AACAAGCCAG AAGACCATGG ATGATTTAAA TTTGAGCACC 
TCTGAGGCTC TGCGGATTGA CCCTGTTCCT AACACCCACC CACTTCTCGT CTTTGTCAAT 
CCTAAGAGTG GCGGGAAGCA GGGGCAAAGG GTGCTCTGGA AGTTCCAGTA TATATTAAAC 
CCTCGACAGG TGTTCAACCT CCTAAAGGAT GGTCCTGAGA TAGGGCTCCG ATTATTCAAG 
GATGTTCCTG ATAGCCGGAT TTTGGTGTGT GGTGGAGACG GCACAGTAGG CTGGATTCTA 
GAGACCATTG ACAAAGCTAA CTTGCCAGTT TTGCCTCCTG TTGCTGTGTT GCCCCTGGGT 
ACTGGAAATG ATCTGGCTCG ATGCCTAAGA TGGGGAGGAG GTTATGAAGG ACAGAATCTG 
GCAAAGATCC TCAAGGATTT AGAGATGAGT AAAGTGGTAC ATATGGATCG ATGGTCTGTG 
GAGGTGATAC CTCAACAAAC TGAAGAAAAA AGTGACCCAG TCCCCTTTCA AATCATCAAT 
AACTACTTCT CTATTGGCGT GGATGCCTCT ATTGCTCATC GATTCCACAT CATGCGAGAG 
AAATATCCGG AGAAGTTCAA CAGCAGAATG AAGAACAAGC TATGGTACTT CGAATTTGCC 
ACATCTGAAT CCATCTTCTC AACATGCAAA AAGCTGGAGG AGTCTTTGAC AGTTGAGATC 
TGTGGGAAAC CGCTGGATCT GAGCAACCTG TCCCTAGAAG GCATCGCAGT GCTAAACATC 
CCTAGCATGC ATGGTGGCTC CAACCTCTGG GGTGATACCA GGAGACCCCA TGGGGATATC 
TATGGGATCA ACCAGGCCTT AGGTGCTACA GCTAAAGTCA TCACCGACCC TGATATCCTG 
AAAACCTGTG TACCAGACCT AAGTGACAAG AGACTGGAAG TGGTTGGGCT GGAGGGTGCA 
ATTGAGATGG GCCAAATCTA TACCAAGCTC AAGAATGCTG GACGTCGGCT GGCCAAGTGC 
TCTGAGATCA CCTTCCACAC CACAAAAACC CTTCCCATGC AAATTGACGG AGAACCCTGG 
ATGCAGACGC CCTGTACAAT CAAGATCACC CACAAGAACC AGATGCCCAT GCTCATGGGC 
CCACCCCCCC GCTCCACCAA TTTCTTTGGC TTCTTGAGCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016874394.1
CDS	652..3213
Translation	MAKERGLISPSDFAQLQKYMEYSTKKVSDVLKLFEDGEMAKYVQGDAIGYEGFQQFLKIYLEVDNVPRHLSLALFQSFETGHCLNETNVTKDVVCLNDVSCYFSLLEGGRPEDKLEFTFKLYDTDRNGILDSSVSWGPCMLGKGICVNLSAPSCPNSSRVLRNRRGWGERLSPLGLNLKVSPKLLTPQRECWIHELGLPYKHTNTFIYLADIPENERCESGVEPRNTDFWKRVWMVVVKGIWLWLLPFLLQEVDKIILQMMRVAEYLDWDVSELRPILQEMMKEIDYDGSGSVSQAEWVRAGATTVPLLVLLGLEMTLKDDGQHMWRPKRFPRPVYCNLCESSIGLGKQGLSCNLCKYTVHDQCAMKALPCEVSTYAKSRKDIGVQSHVWVRGGCESGRCDRCQKKIRIYHSLTGLHCVWCHLEIHDDCLQAVGHECDCGLLRDHILPPSSIYPSVLASGPDRKNSKTSQKTMDDLNLSTSEALRIDPVPNTHPLLVFVNPKSGGKQGQRVLWKFQYILNPRQVFNLLKDGPEIGLRLFKDVPDSRILVCGGDGTVGWILETIDKANLPVLPPVAVLPLGTGNDLARCLRWGGGYEGQNLAKILKDLEMSKVVHMDRWSVEVIPQQTEEKSDPVPFQIINNYFSIGVDASIAHRFHIMREKYPEKFNSRMKNKLWYFEFATSESIFSTCKKLEESLTVEICGKPLDLSNLSLEGIAVLNIPSMHGGSNLWGDTRRPHGDIYGINQALGATAKVITDPDILKTCVPDLSDKRLEVVGLEGAIEMGQIYTKLKNAGRRLAKCSEITFHTTKTLPMQIDGEPWMQTPCTIKITHKNQMPMLMGPPPRSTNFFGFLS

Target ORF information:

RefSeq Version	XM_017018905.1
Organism	Homo sapiens(human)
Definition	Homo sapiens diacylglycerol kinase alpha (DGKA), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017018905.1

ORF Insert Sequence:

ATGGCCAAGG AGAGGGGCCT AATAAGCCCC AGTGATTTTG CCCAGCTGCA AAAATACATG 
GAATACTCCA CCAAAAAGGT CAGTGATGTC CTAAAGCTCT TCGAGGATGG CGAGATGGCT 
AAATATGTCC AAGGAGATGC CATTGGGTAC GAGGGATTCC AGCAATTCCT GAAAATCTAT 
CTCGAAGTGG ATAATGTTCC CAGACACCTA AGCCTGGCAC TGTTTCAATC CTTTGAGACT 
GGTCACTGCT TAAATGAGAC AAATGTGACA AAAGATGTGG TGTGTCTCAA TGATGTTTCC 
TGCTACTTTT CCCTTCTGGA GGGTGGTCGG CCAGAAGACA AGTTAGAATT CACCTTCAAG 
CTGTACGACA CGGACAGAAA TGGGATCCTG GACAGCTCAG TGAGTTGGGG ACCCTGTATG 
CTGGGCAAGG GAATATGTGT TAATTTGTCT GCACCCTCCT GCCCCAACTC TTCCAGGGTC 
TTAAGAAACA GAAGAGGATG GGGGGAGAGG TTGAGTCCTC TAGGACTAAA TTTGAAAGTA 
AGTCCCAAGC TCTTGACCCC TCAAAGAGAA TGCTGGATAC ATGAATTGGG TCTGCCTTAC 
AAACATACAA ACACATTTAT TTACTTGGCA GACATACCTG AGAATGAGAG GTGTGAATCT 
GGGGTGGAAC CCAGGAACAC TGATTTCTGG AAAAGAGTTT GGATGGTGGT AGTAAAGGGG 
ATATGGCTGT GGCTCTTGCC CTTTTTGCTC CAGGAAGTGG ACAAAATTAT CCTACAGATG 
ATGCGAGTGG CTGAATACCT GGATTGGGAT GTGTCTGAGC TGAGGCCGAT TCTTCAGGAG 
ATGATGAAAG AGATTGACTA TGATGGCAGT GGCTCTGTCT CTCAAGCTGA GTGGGTCCGG 
GCTGGGGCCA CCACCGTGCC ACTGCTAGTG CTGCTGGGTC TGGAGATGAC TCTGAAGGAC 
GACGGACAGC ACATGTGGAG GCCCAAGAGG TTCCCCAGAC CAGTCTACTG CAATCTGTGC 
GAGTCAAGCA TTGGTCTTGG CAAACAGGGA CTGAGCTGTA ACCTCTGTAA GTACACTGTT 
CACGACCAGT GTGCCATGAA AGCCCTGCCT TGTGAAGTCA GCACCTATGC CAAGTCTCGG 
AAGGACATTG GTGTCCAATC ACATGTGTGG GTGCGAGGAG GCTGTGAGTC CGGGCGCTGC 
GACCGCTGTC AGAAAAAGAT CCGGATCTAC CACAGTCTGA CCGGGCTGCA TTGTGTATGG 
TGCCACCTAG AGATCCACGA TGACTGCCTG CAAGCGGTGG GCCATGAGTG TGACTGTGGG 
CTGCTCCGGG ATCACATCCT GCCTCCATCT TCCATCTATC CCAGTGTCCT GGCCTCTGGA 
CCGGATCGTA AAAATAGCAA AACAAGCCAG AAGACCATGG ATGATTTAAA TTTGAGCACC 
TCTGAGGCTC TGCGGATTGA CCCTGTTCCT AACACCCACC CACTTCTCGT CTTTGTCAAT 
CCTAAGAGTG GCGGGAAGCA GGGGCAAAGG GTGCTCTGGA AGTTCCAGTA TATATTAAAC 
CCTCGACAGG TGTTCAACCT CCTAAAGGAT GGTCCTGAGA TAGGGCTCCG ATTATTCAAG 
GATGTTCCTG ATAGCCGGAT TTTGGTGTGT GGTGGAGACG GCACAGTAGG CTGGATTCTA 
GAGACCATTG ACAAAGCTAA CTTGCCAGTT TTGCCTCCTG TTGCTGTGTT GCCCCTGGGT 
ACTGGAAATG ATCTGGCTCG ATGCCTAAGA TGGGGAGGAG GTTATGAAGG ACAGAATCTG 
GCAAAGATCC TCAAGGATTT AGAGATGAGT AAAGTGGTAC ATATGGATCG ATGGTCTGTG 
GAGGTGATAC CTCAACAAAC TGAAGAAAAA AGTGACCCAG TCCCCTTTCA AATCATCAAT 
AACTACTTCT CTATTGGCGT GGATGCCTCT ATTGCTCATC GATTCCACAT CATGCGAGAG 
AAATATCCGG AGAAGTTCAA CAGCAGAATG AAGAACAAGC TATGGTACTT CGAATTTGCC 
ACATCTGAAT CCATCTTCTC AACATGCAAA AAGCTGGAGG AGTCTTTGAC AGTTGAGATC 
TGTGGGAAAC CGCTGGATCT GAGCAACCTG TCCCTAGAAG GCATCGCAGT GCTAAACATC 
CCTAGCATGC ATGGTGGCTC CAACCTCTGG GGTGATACCA GGAGACCCCA TGGGGATATC 
TATGGGATCA ACCAGGCCTT AGGTGCTACA GCTAAAGTCA TCACCGACCC TGATATCCTG 
AAAACCTGTG TACCAGACCT AAGTGACAAG AGACTGGAAG TGGTTGGGCT GGAGGGTGCA 
ATTGAGATGG GCCAAATCTA TACCAAGCTC AAGAATGCTG GACGTCGGCT GGCCAAGTGC 
TCTGAGATCA CCTTCCACAC CACAAAAACC CTTCCCATGC AAATTGACGG AGAACCCTGG 
ATGCAGACGC CCTGTACAAT CAAGATCACC CACAAGAACC AGATGCCCAT GCTCATGGGC 
CCACCCCCCC GCTCCACCAA TTTCTTTGGC TTCTTGAGCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DGKA ( XM_017018906.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016874395.1 Homo sapiens diacylglycerol kinase alpha (DGKA), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OHu83568
Clone ID Related Accession (Same CDS sequence)	XM_017018903.1 , XM_017018904.1 , XM_017018901.2 , XM_017018907.1 , XM_017018906.1 , XM_017018905.1 , XM_017018906.2 , XM_017018901.1 , XM_017018902.1
Accession Version	XM_017018906.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2562bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	diacylglycerol kinase alpha isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017018906.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAGG AGAGGGGCCT AATAAGCCCC AGTGATTTTG CCCAGCTGCA AAAATACATG 
GAATACTCCA CCAAAAAGGT CAGTGATGTC CTAAAGCTCT TCGAGGATGG CGAGATGGCT 
AAATATGTCC AAGGAGATGC CATTGGGTAC GAGGGATTCC AGCAATTCCT GAAAATCTAT 
CTCGAAGTGG ATAATGTTCC CAGACACCTA AGCCTGGCAC TGTTTCAATC CTTTGAGACT 
GGTCACTGCT TAAATGAGAC AAATGTGACA AAAGATGTGG TGTGTCTCAA TGATGTTTCC 
TGCTACTTTT CCCTTCTGGA GGGTGGTCGG CCAGAAGACA AGTTAGAATT CACCTTCAAG 
CTGTACGACA CGGACAGAAA TGGGATCCTG GACAGCTCAG TGAGTTGGGG ACCCTGTATG 
CTGGGCAAGG GAATATGTGT TAATTTGTCT GCACCCTCCT GCCCCAACTC TTCCAGGGTC 
TTAAGAAACA GAAGAGGATG GGGGGAGAGG TTGAGTCCTC TAGGACTAAA TTTGAAAGTA 
AGTCCCAAGC TCTTGACCCC TCAAAGAGAA TGCTGGATAC ATGAATTGGG TCTGCCTTAC 
AAACATACAA ACACATTTAT TTACTTGGCA GACATACCTG AGAATGAGAG GTGTGAATCT 
GGGGTGGAAC CCAGGAACAC TGATTTCTGG AAAAGAGTTT GGATGGTGGT AGTAAAGGGG 
ATATGGCTGT GGCTCTTGCC CTTTTTGCTC CAGGAAGTGG ACAAAATTAT CCTACAGATG 
ATGCGAGTGG CTGAATACCT GGATTGGGAT GTGTCTGAGC TGAGGCCGAT TCTTCAGGAG 
ATGATGAAAG AGATTGACTA TGATGGCAGT GGCTCTGTCT CTCAAGCTGA GTGGGTCCGG 
GCTGGGGCCA CCACCGTGCC ACTGCTAGTG CTGCTGGGTC TGGAGATGAC TCTGAAGGAC 
GACGGACAGC ACATGTGGAG GCCCAAGAGG TTCCCCAGAC CAGTCTACTG CAATCTGTGC 
GAGTCAAGCA TTGGTCTTGG CAAACAGGGA CTGAGCTGTA ACCTCTGTAA GTACACTGTT 
CACGACCAGT GTGCCATGAA AGCCCTGCCT TGTGAAGTCA GCACCTATGC CAAGTCTCGG 
AAGGACATTG GTGTCCAATC ACATGTGTGG GTGCGAGGAG GCTGTGAGTC CGGGCGCTGC 
GACCGCTGTC AGAAAAAGAT CCGGATCTAC CACAGTCTGA CCGGGCTGCA TTGTGTATGG 
TGCCACCTAG AGATCCACGA TGACTGCCTG CAAGCGGTGG GCCATGAGTG TGACTGTGGG 
CTGCTCCGGG ATCACATCCT GCCTCCATCT TCCATCTATC CCAGTGTCCT GGCCTCTGGA 
CCGGATCGTA AAAATAGCAA AACAAGCCAG AAGACCATGG ATGATTTAAA TTTGAGCACC 
TCTGAGGCTC TGCGGATTGA CCCTGTTCCT AACACCCACC CACTTCTCGT CTTTGTCAAT 
CCTAAGAGTG GCGGGAAGCA GGGGCAAAGG GTGCTCTGGA AGTTCCAGTA TATATTAAAC 
CCTCGACAGG TGTTCAACCT CCTAAAGGAT GGTCCTGAGA TAGGGCTCCG ATTATTCAAG 
GATGTTCCTG ATAGCCGGAT TTTGGTGTGT GGTGGAGACG GCACAGTAGG CTGGATTCTA 
GAGACCATTG ACAAAGCTAA CTTGCCAGTT TTGCCTCCTG TTGCTGTGTT GCCCCTGGGT 
ACTGGAAATG ATCTGGCTCG ATGCCTAAGA TGGGGAGGAG GTTATGAAGG ACAGAATCTG 
GCAAAGATCC TCAAGGATTT AGAGATGAGT AAAGTGGTAC ATATGGATCG ATGGTCTGTG 
GAGGTGATAC CTCAACAAAC TGAAGAAAAA AGTGACCCAG TCCCCTTTCA AATCATCAAT 
AACTACTTCT CTATTGGCGT GGATGCCTCT ATTGCTCATC GATTCCACAT CATGCGAGAG 
AAATATCCGG AGAAGTTCAA CAGCAGAATG AAGAACAAGC TATGGTACTT CGAATTTGCC 
ACATCTGAAT CCATCTTCTC AACATGCAAA AAGCTGGAGG AGTCTTTGAC AGTTGAGATC 
TGTGGGAAAC CGCTGGATCT GAGCAACCTG TCCCTAGAAG GCATCGCAGT GCTAAACATC 
CCTAGCATGC ATGGTGGCTC CAACCTCTGG GGTGATACCA GGAGACCCCA TGGGGATATC 
TATGGGATCA ACCAGGCCTT AGGTGCTACA GCTAAAGTCA TCACCGACCC TGATATCCTG 
AAAACCTGTG TACCAGACCT AAGTGACAAG AGACTGGAAG TGGTTGGGCT GGAGGGTGCA 
ATTGAGATGG GCCAAATCTA TACCAAGCTC AAGAATGCTG GACGTCGGCT GGCCAAGTGC 
TCTGAGATCA CCTTCCACAC CACAAAAACC CTTCCCATGC AAATTGACGG AGAACCCTGG 
ATGCAGACGC CCTGTACAAT CAAGATCACC CACAAGAACC AGATGCCCAT GCTCATGGGC 
CCACCCCCCC GCTCCACCAA TTTCTTTGGC TTCTTGAGCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016874395.1
CDS	275..2836
Translation	MAKERGLISPSDFAQLQKYMEYSTKKVSDVLKLFEDGEMAKYVQGDAIGYEGFQQFLKIYLEVDNVPRHLSLALFQSFETGHCLNETNVTKDVVCLNDVSCYFSLLEGGRPEDKLEFTFKLYDTDRNGILDSSVSWGPCMLGKGICVNLSAPSCPNSSRVLRNRRGWGERLSPLGLNLKVSPKLLTPQRECWIHELGLPYKHTNTFIYLADIPENERCESGVEPRNTDFWKRVWMVVVKGIWLWLLPFLLQEVDKIILQMMRVAEYLDWDVSELRPILQEMMKEIDYDGSGSVSQAEWVRAGATTVPLLVLLGLEMTLKDDGQHMWRPKRFPRPVYCNLCESSIGLGKQGLSCNLCKYTVHDQCAMKALPCEVSTYAKSRKDIGVQSHVWVRGGCESGRCDRCQKKIRIYHSLTGLHCVWCHLEIHDDCLQAVGHECDCGLLRDHILPPSSIYPSVLASGPDRKNSKTSQKTMDDLNLSTSEALRIDPVPNTHPLLVFVNPKSGGKQGQRVLWKFQYILNPRQVFNLLKDGPEIGLRLFKDVPDSRILVCGGDGTVGWILETIDKANLPVLPPVAVLPLGTGNDLARCLRWGGGYEGQNLAKILKDLEMSKVVHMDRWSVEVIPQQTEEKSDPVPFQIINNYFSIGVDASIAHRFHIMREKYPEKFNSRMKNKLWYFEFATSESIFSTCKKLEESLTVEICGKPLDLSNLSLEGIAVLNIPSMHGGSNLWGDTRRPHGDIYGINQALGATAKVITDPDILKTCVPDLSDKRLEVVGLEGAIEMGQIYTKLKNAGRRLAKCSEITFHTTKTLPMQIDGEPWMQTPCTIKITHKNQMPMLMGPPPRSTNFFGFLS

Target ORF information:

RefSeq Version	XM_017018906.2
Organism	Homo sapiens(human)
Definition	Homo sapiens diacylglycerol kinase alpha (DGKA), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017018906.2

ORF Insert Sequence:

ATGGCCAAGG AGAGGGGCCT AATAAGCCCC AGTGATTTTG CCCAGCTGCA AAAATACATG 
GAATACTCCA CCAAAAAGGT CAGTGATGTC CTAAAGCTCT TCGAGGATGG CGAGATGGCT 
AAATATGTCC AAGGAGATGC CATTGGGTAC GAGGGATTCC AGCAATTCCT GAAAATCTAT 
CTCGAAGTGG ATAATGTTCC CAGACACCTA AGCCTGGCAC TGTTTCAATC CTTTGAGACT 
GGTCACTGCT TAAATGAGAC AAATGTGACA AAAGATGTGG TGTGTCTCAA TGATGTTTCC 
TGCTACTTTT CCCTTCTGGA GGGTGGTCGG CCAGAAGACA AGTTAGAATT CACCTTCAAG 
CTGTACGACA CGGACAGAAA TGGGATCCTG GACAGCTCAG TGAGTTGGGG ACCCTGTATG 
CTGGGCAAGG GAATATGTGT TAATTTGTCT GCACCCTCCT GCCCCAACTC TTCCAGGGTC 
TTAAGAAACA GAAGAGGATG GGGGGAGAGG TTGAGTCCTC TAGGACTAAA TTTGAAAGTA 
AGTCCCAAGC TCTTGACCCC TCAAAGAGAA TGCTGGATAC ATGAATTGGG TCTGCCTTAC 
AAACATACAA ACACATTTAT TTACTTGGCA GACATACCTG AGAATGAGAG GTGTGAATCT 
GGGGTGGAAC CCAGGAACAC TGATTTCTGG AAAAGAGTTT GGATGGTGGT AGTAAAGGGG 
ATATGGCTGT GGCTCTTGCC CTTTTTGCTC CAGGAAGTGG ACAAAATTAT CCTACAGATG 
ATGCGAGTGG CTGAATACCT GGATTGGGAT GTGTCTGAGC TGAGGCCGAT TCTTCAGGAG 
ATGATGAAAG AGATTGACTA TGATGGCAGT GGCTCTGTCT CTCAAGCTGA GTGGGTCCGG 
GCTGGGGCCA CCACCGTGCC ACTGCTAGTG CTGCTGGGTC TGGAGATGAC TCTGAAGGAC 
GACGGACAGC ACATGTGGAG GCCCAAGAGG TTCCCCAGAC CAGTCTACTG CAATCTGTGC 
GAGTCAAGCA TTGGTCTTGG CAAACAGGGA CTGAGCTGTA ACCTCTGTAA GTACACTGTT 
CACGACCAGT GTGCCATGAA AGCCCTGCCT TGTGAAGTCA GCACCTATGC CAAGTCTCGG 
AAGGACATTG GTGTCCAATC ACATGTGTGG GTGCGAGGAG GCTGTGAGTC CGGGCGCTGC 
GACCGCTGTC AGAAAAAGAT CCGGATCTAC CACAGTCTGA CCGGGCTGCA TTGTGTATGG 
TGCCACCTAG AGATCCACGA TGACTGCCTG CAAGCGGTGG GCCATGAGTG TGACTGTGGG 
CTGCTCCGGG ATCACATCCT GCCTCCATCT TCCATCTATC CCAGTGTCCT GGCCTCTGGA 
CCGGATCGTA AAAATAGCAA AACAAGCCAG AAGACCATGG ATGATTTAAA TTTGAGCACC 
TCTGAGGCTC TGCGGATTGA CCCTGTTCCT AACACCCACC CACTTCTCGT CTTTGTCAAT 
CCTAAGAGTG GCGGGAAGCA GGGGCAAAGG GTGCTCTGGA AGTTCCAGTA TATATTAAAC 
CCTCGACAGG TGTTCAACCT CCTAAAGGAT GGTCCTGAGA TAGGGCTCCG ATTATTCAAG 
GATGTTCCTG ATAGCCGGAT TTTGGTGTGT GGTGGAGACG GCACAGTAGG CTGGATTCTA 
GAGACCATTG ACAAAGCTAA CTTGCCAGTT TTGCCTCCTG TTGCTGTGTT GCCCCTGGGT 
ACTGGAAATG ATCTGGCTCG ATGCCTAAGA TGGGGAGGAG GTTATGAAGG ACAGAATCTG 
GCAAAGATCC TCAAGGATTT AGAGATGAGT AAAGTGGTAC ATATGGATCG ATGGTCTGTG 
GAGGTGATAC CTCAACAAAC TGAAGAAAAA AGTGACCCAG TCCCCTTTCA AATCATCAAT 
AACTACTTCT CTATTGGCGT GGATGCCTCT ATTGCTCATC GATTCCACAT CATGCGAGAG 
AAATATCCGG AGAAGTTCAA CAGCAGAATG AAGAACAAGC TATGGTACTT CGAATTTGCC 
ACATCTGAAT CCATCTTCTC AACATGCAAA AAGCTGGAGG AGTCTTTGAC AGTTGAGATC 
TGTGGGAAAC CGCTGGATCT GAGCAACCTG TCCCTAGAAG GCATCGCAGT GCTAAACATC 
CCTAGCATGC ATGGTGGCTC CAACCTCTGG GGTGATACCA GGAGACCCCA TGGGGATATC 
TATGGGATCA ACCAGGCCTT AGGTGCTACA GCTAAAGTCA TCACCGACCC TGATATCCTG 
AAAACCTGTG TACCAGACCT AAGTGACAAG AGACTGGAAG TGGTTGGGCT GGAGGGTGCA 
ATTGAGATGG GCCAAATCTA TACCAAGCTC AAGAATGCTG GACGTCGGCT GGCCAAGTGC 
TCTGAGATCA CCTTCCACAC CACAAAAACC CTTCCCATGC AAATTGACGG AGAACCCTGG 
ATGCAGACGC CCTGTACAAT CAAGATCACC CACAAGAACC AGATGCCCAT GCTCATGGGC 
CCACCCCCCC GCTCCACCAA TTTCTTTGGC TTCTTGAGCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DGKA ( XM_017018901.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016874390.1 Homo sapiens diacylglycerol kinase alpha (DGKA), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu83568
Clone ID Related Accession (Same CDS sequence)	XM_017018903.1 , XM_017018904.1 , XM_017018901.2 , XM_017018907.1 , XM_017018906.1 , XM_017018905.1 , XM_017018906.2 , XM_017018901.1 , XM_017018902.1
Accession Version	XM_017018901.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2562bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	diacylglycerol kinase alpha isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAGG AGAGGGGCCT AATAAGCCCC AGTGATTTTG CCCAGCTGCA AAAATACATG 
GAATACTCCA CCAAAAAGGT CAGTGATGTC CTAAAGCTCT TCGAGGATGG CGAGATGGCT 
AAATATGTCC AAGGAGATGC CATTGGGTAC GAGGGATTCC AGCAATTCCT GAAAATCTAT 
CTCGAAGTGG ATAATGTTCC CAGACACCTA AGCCTGGCAC TGTTTCAATC CTTTGAGACT 
GGTCACTGCT TAAATGAGAC AAATGTGACA AAAGATGTGG TGTGTCTCAA TGATGTTTCC 
TGCTACTTTT CCCTTCTGGA GGGTGGTCGG CCAGAAGACA AGTTAGAATT CACCTTCAAG 
CTGTACGACA CGGACAGAAA TGGGATCCTG GACAGCTCAG TGAGTTGGGG ACCCTGTATG 
CTGGGCAAGG GAATATGTGT TAATTTGTCT GCACCCTCCT GCCCCAACTC TTCCAGGGTC 
TTAAGAAACA GAAGAGGATG GGGGGAGAGG TTGAGTCCTC TAGGACTAAA TTTGAAAGTA 
AGTCCCAAGC TCTTGACCCC TCAAAGAGAA TGCTGGATAC ATGAATTGGG TCTGCCTTAC 
AAACATACAA ACACATTTAT TTACTTGGCA GACATACCTG AGAATGAGAG GTGTGAATCT 
GGGGTGGAAC CCAGGAACAC TGATTTCTGG AAAAGAGTTT GGATGGTGGT AGTAAAGGGG 
ATATGGCTGT GGCTCTTGCC CTTTTTGCTC CAGGAAGTGG ACAAAATTAT CCTACAGATG 
ATGCGAGTGG CTGAATACCT GGATTGGGAT GTGTCTGAGC TGAGGCCGAT TCTTCAGGAG 
ATGATGAAAG AGATTGACTA TGATGGCAGT GGCTCTGTCT CTCAAGCTGA GTGGGTCCGG 
GCTGGGGCCA CCACCGTGCC ACTGCTAGTG CTGCTGGGTC TGGAGATGAC TCTGAAGGAC 
GACGGACAGC ACATGTGGAG GCCCAAGAGG TTCCCCAGAC CAGTCTACTG CAATCTGTGC 
GAGTCAAGCA TTGGTCTTGG CAAACAGGGA CTGAGCTGTA ACCTCTGTAA GTACACTGTT 
CACGACCAGT GTGCCATGAA AGCCCTGCCT TGTGAAGTCA GCACCTATGC CAAGTCTCGG 
AAGGACATTG GTGTCCAATC ACATGTGTGG GTGCGAGGAG GCTGTGAGTC CGGGCGCTGC 
GACCGCTGTC AGAAAAAGAT CCGGATCTAC CACAGTCTGA CCGGGCTGCA TTGTGTATGG 
TGCCACCTAG AGATCCACGA TGACTGCCTG CAAGCGGTGG GCCATGAGTG TGACTGTGGG 
CTGCTCCGGG ATCACATCCT GCCTCCATCT TCCATCTATC CCAGTGTCCT GGCCTCTGGA 
CCGGATCGTA AAAATAGCAA AACAAGCCAG AAGACCATGG ATGATTTAAA TTTGAGCACC 
TCTGAGGCTC TGCGGATTGA CCCTGTTCCT AACACCCACC CACTTCTCGT CTTTGTCAAT 
CCTAAGAGTG GCGGGAAGCA GGGGCAAAGG GTGCTCTGGA AGTTCCAGTA TATATTAAAC 
CCTCGACAGG TGTTCAACCT CCTAAAGGAT GGTCCTGAGA TAGGGCTCCG ATTATTCAAG 
GATGTTCCTG ATAGCCGGAT TTTGGTGTGT GGTGGAGACG GCACAGTAGG CTGGATTCTA 
GAGACCATTG ACAAAGCTAA CTTGCCAGTT TTGCCTCCTG TTGCTGTGTT GCCCCTGGGT 
ACTGGAAATG ATCTGGCTCG ATGCCTAAGA TGGGGAGGAG GTTATGAAGG ACAGAATCTG 
GCAAAGATCC TCAAGGATTT AGAGATGAGT AAAGTGGTAC ATATGGATCG ATGGTCTGTG 
GAGGTGATAC CTCAACAAAC TGAAGAAAAA AGTGACCCAG TCCCCTTTCA AATCATCAAT 
AACTACTTCT CTATTGGCGT GGATGCCTCT ATTGCTCATC GATTCCACAT CATGCGAGAG 
AAATATCCGG AGAAGTTCAA CAGCAGAATG AAGAACAAGC TATGGTACTT CGAATTTGCC 
ACATCTGAAT CCATCTTCTC AACATGCAAA AAGCTGGAGG AGTCTTTGAC AGTTGAGATC 
TGTGGGAAAC CGCTGGATCT GAGCAACCTG TCCCTAGAAG GCATCGCAGT GCTAAACATC 
CCTAGCATGC ATGGTGGCTC CAACCTCTGG GGTGATACCA GGAGACCCCA TGGGGATATC 
TATGGGATCA ACCAGGCCTT AGGTGCTACA GCTAAAGTCA TCACCGACCC TGATATCCTG 
AAAACCTGTG TACCAGACCT AAGTGACAAG AGACTGGAAG TGGTTGGGCT GGAGGGTGCA 
ATTGAGATGG GCCAAATCTA TACCAAGCTC AAGAATGCTG GACGTCGGCT GGCCAAGTGC 
TCTGAGATCA CCTTCCACAC CACAAAAACC CTTCCCATGC AAATTGACGG AGAACCCTGG 
ATGCAGACGC CCTGTACAAT CAAGATCACC CACAAGAACC AGATGCCCAT GCTCATGGGC 
CCACCCCCCC GCTCCACCAA TTTCTTTGGC TTCTTGAGCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016874390.1
CDS	351..2912
Translation	MAKERGLISPSDFAQLQKYMEYSTKKVSDVLKLFEDGEMAKYVQGDAIGYEGFQQFLKIYLEVDNVPRHLSLALFQSFETGHCLNETNVTKDVVCLNDVSCYFSLLEGGRPEDKLEFTFKLYDTDRNGILDSSVSWGPCMLGKGICVNLSAPSCPNSSRVLRNRRGWGERLSPLGLNLKVSPKLLTPQRECWIHELGLPYKHTNTFIYLADIPENERCESGVEPRNTDFWKRVWMVVVKGIWLWLLPFLLQEVDKIILQMMRVAEYLDWDVSELRPILQEMMKEIDYDGSGSVSQAEWVRAGATTVPLLVLLGLEMTLKDDGQHMWRPKRFPRPVYCNLCESSIGLGKQGLSCNLCKYTVHDQCAMKALPCEVSTYAKSRKDIGVQSHVWVRGGCESGRCDRCQKKIRIYHSLTGLHCVWCHLEIHDDCLQAVGHECDCGLLRDHILPPSSIYPSVLASGPDRKNSKTSQKTMDDLNLSTSEALRIDPVPNTHPLLVFVNPKSGGKQGQRVLWKFQYILNPRQVFNLLKDGPEIGLRLFKDVPDSRILVCGGDGTVGWILETIDKANLPVLPPVAVLPLGTGNDLARCLRWGGGYEGQNLAKILKDLEMSKVVHMDRWSVEVIPQQTEEKSDPVPFQIINNYFSIGVDASIAHRFHIMREKYPEKFNSRMKNKLWYFEFATSESIFSTCKKLEESLTVEICGKPLDLSNLSLEGIAVLNIPSMHGGSNLWGDTRRPHGDIYGINQALGATAKVITDPDILKTCVPDLSDKRLEVVGLEGAIEMGQIYTKLKNAGRRLAKCSEITFHTTKTLPMQIDGEPWMQTPCTIKITHKNQMPMLMGPPPRSTNFFGFLS

Target ORF information:

RefSeq Version	XM_017018901.1
Organism	Homo sapiens(human)
Definition	Homo sapiens diacylglycerol kinase alpha (DGKA), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017018901.1

ORF Insert Sequence:

ATGGCCAAGG AGAGGGGCCT AATAAGCCCC AGTGATTTTG CCCAGCTGCA AAAATACATG 
GAATACTCCA CCAAAAAGGT CAGTGATGTC CTAAAGCTCT TCGAGGATGG CGAGATGGCT 
AAATATGTCC AAGGAGATGC CATTGGGTAC GAGGGATTCC AGCAATTCCT GAAAATCTAT 
CTCGAAGTGG ATAATGTTCC CAGACACCTA AGCCTGGCAC TGTTTCAATC CTTTGAGACT 
GGTCACTGCT TAAATGAGAC AAATGTGACA AAAGATGTGG TGTGTCTCAA TGATGTTTCC 
TGCTACTTTT CCCTTCTGGA GGGTGGTCGG CCAGAAGACA AGTTAGAATT CACCTTCAAG 
CTGTACGACA CGGACAGAAA TGGGATCCTG GACAGCTCAG TGAGTTGGGG ACCCTGTATG 
CTGGGCAAGG GAATATGTGT TAATTTGTCT GCACCCTCCT GCCCCAACTC TTCCAGGGTC 
TTAAGAAACA GAAGAGGATG GGGGGAGAGG TTGAGTCCTC TAGGACTAAA TTTGAAAGTA 
AGTCCCAAGC TCTTGACCCC TCAAAGAGAA TGCTGGATAC ATGAATTGGG TCTGCCTTAC 
AAACATACAA ACACATTTAT TTACTTGGCA GACATACCTG AGAATGAGAG GTGTGAATCT 
GGGGTGGAAC CCAGGAACAC TGATTTCTGG AAAAGAGTTT GGATGGTGGT AGTAAAGGGG 
ATATGGCTGT GGCTCTTGCC CTTTTTGCTC CAGGAAGTGG ACAAAATTAT CCTACAGATG 
ATGCGAGTGG CTGAATACCT GGATTGGGAT GTGTCTGAGC TGAGGCCGAT TCTTCAGGAG 
ATGATGAAAG AGATTGACTA TGATGGCAGT GGCTCTGTCT CTCAAGCTGA GTGGGTCCGG 
GCTGGGGCCA CCACCGTGCC ACTGCTAGTG CTGCTGGGTC TGGAGATGAC TCTGAAGGAC 
GACGGACAGC ACATGTGGAG GCCCAAGAGG TTCCCCAGAC CAGTCTACTG CAATCTGTGC 
GAGTCAAGCA TTGGTCTTGG CAAACAGGGA CTGAGCTGTA ACCTCTGTAA GTACACTGTT 
CACGACCAGT GTGCCATGAA AGCCCTGCCT TGTGAAGTCA GCACCTATGC CAAGTCTCGG 
AAGGACATTG GTGTCCAATC ACATGTGTGG GTGCGAGGAG GCTGTGAGTC CGGGCGCTGC 
GACCGCTGTC AGAAAAAGAT CCGGATCTAC CACAGTCTGA CCGGGCTGCA TTGTGTATGG 
TGCCACCTAG AGATCCACGA TGACTGCCTG CAAGCGGTGG GCCATGAGTG TGACTGTGGG 
CTGCTCCGGG ATCACATCCT GCCTCCATCT TCCATCTATC CCAGTGTCCT GGCCTCTGGA 
CCGGATCGTA AAAATAGCAA AACAAGCCAG AAGACCATGG ATGATTTAAA TTTGAGCACC 
TCTGAGGCTC TGCGGATTGA CCCTGTTCCT AACACCCACC CACTTCTCGT CTTTGTCAAT 
CCTAAGAGTG GCGGGAAGCA GGGGCAAAGG GTGCTCTGGA AGTTCCAGTA TATATTAAAC 
CCTCGACAGG TGTTCAACCT CCTAAAGGAT GGTCCTGAGA TAGGGCTCCG ATTATTCAAG 
GATGTTCCTG ATAGCCGGAT TTTGGTGTGT GGTGGAGACG GCACAGTAGG CTGGATTCTA 
GAGACCATTG ACAAAGCTAA CTTGCCAGTT TTGCCTCCTG TTGCTGTGTT GCCCCTGGGT 
ACTGGAAATG ATCTGGCTCG ATGCCTAAGA TGGGGAGGAG GTTATGAAGG ACAGAATCTG 
GCAAAGATCC TCAAGGATTT AGAGATGAGT AAAGTGGTAC ATATGGATCG ATGGTCTGTG 
GAGGTGATAC CTCAACAAAC TGAAGAAAAA AGTGACCCAG TCCCCTTTCA AATCATCAAT 
AACTACTTCT CTATTGGCGT GGATGCCTCT ATTGCTCATC GATTCCACAT CATGCGAGAG 
AAATATCCGG AGAAGTTCAA CAGCAGAATG AAGAACAAGC TATGGTACTT CGAATTTGCC 
ACATCTGAAT CCATCTTCTC AACATGCAAA AAGCTGGAGG AGTCTTTGAC AGTTGAGATC 
TGTGGGAAAC CGCTGGATCT GAGCAACCTG TCCCTAGAAG GCATCGCAGT GCTAAACATC 
CCTAGCATGC ATGGTGGCTC CAACCTCTGG GGTGATACCA GGAGACCCCA TGGGGATATC 
TATGGGATCA ACCAGGCCTT AGGTGCTACA GCTAAAGTCA TCACCGACCC TGATATCCTG 
AAAACCTGTG TACCAGACCT AAGTGACAAG AGACTGGAAG TGGTTGGGCT GGAGGGTGCA 
ATTGAGATGG GCCAAATCTA TACCAAGCTC AAGAATGCTG GACGTCGGCT GGCCAAGTGC 
TCTGAGATCA CCTTCCACAC CACAAAAACC CTTCCCATGC AAATTGACGG AGAACCCTGG 
ATGCAGACGC CCTGTACAAT CAAGATCACC CACAAGAACC AGATGCCCAT GCTCATGGGC 
CCACCCCCCC GCTCCACCAA TTTCTTTGGC TTCTTGAGCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DGKA ( XM_017018902.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016874391.1 Homo sapiens diacylglycerol kinase alpha (DGKA), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHu83568
Clone ID Related Accession (Same CDS sequence)	XM_017018903.1 , XM_017018904.1 , XM_017018901.2 , XM_017018907.1 , XM_017018906.1 , XM_017018905.1 , XM_017018906.2 , XM_017018901.1 , XM_017018902.1
Accession Version	XM_017018902.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2562bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	diacylglycerol kinase alpha isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAGG AGAGGGGCCT AATAAGCCCC AGTGATTTTG CCCAGCTGCA AAAATACATG 
GAATACTCCA CCAAAAAGGT CAGTGATGTC CTAAAGCTCT TCGAGGATGG CGAGATGGCT 
AAATATGTCC AAGGAGATGC CATTGGGTAC GAGGGATTCC AGCAATTCCT GAAAATCTAT 
CTCGAAGTGG ATAATGTTCC CAGACACCTA AGCCTGGCAC TGTTTCAATC CTTTGAGACT 
GGTCACTGCT TAAATGAGAC AAATGTGACA AAAGATGTGG TGTGTCTCAA TGATGTTTCC 
TGCTACTTTT CCCTTCTGGA GGGTGGTCGG CCAGAAGACA AGTTAGAATT CACCTTCAAG 
CTGTACGACA CGGACAGAAA TGGGATCCTG GACAGCTCAG TGAGTTGGGG ACCCTGTATG 
CTGGGCAAGG GAATATGTGT TAATTTGTCT GCACCCTCCT GCCCCAACTC TTCCAGGGTC 
TTAAGAAACA GAAGAGGATG GGGGGAGAGG TTGAGTCCTC TAGGACTAAA TTTGAAAGTA 
AGTCCCAAGC TCTTGACCCC TCAAAGAGAA TGCTGGATAC ATGAATTGGG TCTGCCTTAC 
AAACATACAA ACACATTTAT TTACTTGGCA GACATACCTG AGAATGAGAG GTGTGAATCT 
GGGGTGGAAC CCAGGAACAC TGATTTCTGG AAAAGAGTTT GGATGGTGGT AGTAAAGGGG 
ATATGGCTGT GGCTCTTGCC CTTTTTGCTC CAGGAAGTGG ACAAAATTAT CCTACAGATG 
ATGCGAGTGG CTGAATACCT GGATTGGGAT GTGTCTGAGC TGAGGCCGAT TCTTCAGGAG 
ATGATGAAAG AGATTGACTA TGATGGCAGT GGCTCTGTCT CTCAAGCTGA GTGGGTCCGG 
GCTGGGGCCA CCACCGTGCC ACTGCTAGTG CTGCTGGGTC TGGAGATGAC TCTGAAGGAC 
GACGGACAGC ACATGTGGAG GCCCAAGAGG TTCCCCAGAC CAGTCTACTG CAATCTGTGC 
GAGTCAAGCA TTGGTCTTGG CAAACAGGGA CTGAGCTGTA ACCTCTGTAA GTACACTGTT 
CACGACCAGT GTGCCATGAA AGCCCTGCCT TGTGAAGTCA GCACCTATGC CAAGTCTCGG 
AAGGACATTG GTGTCCAATC ACATGTGTGG GTGCGAGGAG GCTGTGAGTC CGGGCGCTGC 
GACCGCTGTC AGAAAAAGAT CCGGATCTAC CACAGTCTGA CCGGGCTGCA TTGTGTATGG 
TGCCACCTAG AGATCCACGA TGACTGCCTG CAAGCGGTGG GCCATGAGTG TGACTGTGGG 
CTGCTCCGGG ATCACATCCT GCCTCCATCT TCCATCTATC CCAGTGTCCT GGCCTCTGGA 
CCGGATCGTA AAAATAGCAA AACAAGCCAG AAGACCATGG ATGATTTAAA TTTGAGCACC 
TCTGAGGCTC TGCGGATTGA CCCTGTTCCT AACACCCACC CACTTCTCGT CTTTGTCAAT 
CCTAAGAGTG GCGGGAAGCA GGGGCAAAGG GTGCTCTGGA AGTTCCAGTA TATATTAAAC 
CCTCGACAGG TGTTCAACCT CCTAAAGGAT GGTCCTGAGA TAGGGCTCCG ATTATTCAAG 
GATGTTCCTG ATAGCCGGAT TTTGGTGTGT GGTGGAGACG GCACAGTAGG CTGGATTCTA 
GAGACCATTG ACAAAGCTAA CTTGCCAGTT TTGCCTCCTG TTGCTGTGTT GCCCCTGGGT 
ACTGGAAATG ATCTGGCTCG ATGCCTAAGA TGGGGAGGAG GTTATGAAGG ACAGAATCTG 
GCAAAGATCC TCAAGGATTT AGAGATGAGT AAAGTGGTAC ATATGGATCG ATGGTCTGTG 
GAGGTGATAC CTCAACAAAC TGAAGAAAAA AGTGACCCAG TCCCCTTTCA AATCATCAAT 
AACTACTTCT CTATTGGCGT GGATGCCTCT ATTGCTCATC GATTCCACAT CATGCGAGAG 
AAATATCCGG AGAAGTTCAA CAGCAGAATG AAGAACAAGC TATGGTACTT CGAATTTGCC 
ACATCTGAAT CCATCTTCTC AACATGCAAA AAGCTGGAGG AGTCTTTGAC AGTTGAGATC 
TGTGGGAAAC CGCTGGATCT GAGCAACCTG TCCCTAGAAG GCATCGCAGT GCTAAACATC 
CCTAGCATGC ATGGTGGCTC CAACCTCTGG GGTGATACCA GGAGACCCCA TGGGGATATC 
TATGGGATCA ACCAGGCCTT AGGTGCTACA GCTAAAGTCA TCACCGACCC TGATATCCTG 
AAAACCTGTG TACCAGACCT AAGTGACAAG AGACTGGAAG TGGTTGGGCT GGAGGGTGCA 
ATTGAGATGG GCCAAATCTA TACCAAGCTC AAGAATGCTG GACGTCGGCT GGCCAAGTGC 
TCTGAGATCA CCTTCCACAC CACAAAAACC CTTCCCATGC AAATTGACGG AGAACCCTGG 
ATGCAGACGC CCTGTACAAT CAAGATCACC CACAAGAACC AGATGCCCAT GCTCATGGGC 
CCACCCCCCC GCTCCACCAA TTTCTTTGGC TTCTTGAGCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016874391.1
CDS	173..2734
Translation	MAKERGLISPSDFAQLQKYMEYSTKKVSDVLKLFEDGEMAKYVQGDAIGYEGFQQFLKIYLEVDNVPRHLSLALFQSFETGHCLNETNVTKDVVCLNDVSCYFSLLEGGRPEDKLEFTFKLYDTDRNGILDSSVSWGPCMLGKGICVNLSAPSCPNSSRVLRNRRGWGERLSPLGLNLKVSPKLLTPQRECWIHELGLPYKHTNTFIYLADIPENERCESGVEPRNTDFWKRVWMVVVKGIWLWLLPFLLQEVDKIILQMMRVAEYLDWDVSELRPILQEMMKEIDYDGSGSVSQAEWVRAGATTVPLLVLLGLEMTLKDDGQHMWRPKRFPRPVYCNLCESSIGLGKQGLSCNLCKYTVHDQCAMKALPCEVSTYAKSRKDIGVQSHVWVRGGCESGRCDRCQKKIRIYHSLTGLHCVWCHLEIHDDCLQAVGHECDCGLLRDHILPPSSIYPSVLASGPDRKNSKTSQKTMDDLNLSTSEALRIDPVPNTHPLLVFVNPKSGGKQGQRVLWKFQYILNPRQVFNLLKDGPEIGLRLFKDVPDSRILVCGGDGTVGWILETIDKANLPVLPPVAVLPLGTGNDLARCLRWGGGYEGQNLAKILKDLEMSKVVHMDRWSVEVIPQQTEEKSDPVPFQIINNYFSIGVDASIAHRFHIMREKYPEKFNSRMKNKLWYFEFATSESIFSTCKKLEESLTVEICGKPLDLSNLSLEGIAVLNIPSMHGGSNLWGDTRRPHGDIYGINQALGATAKVITDPDILKTCVPDLSDKRLEVVGLEGAIEMGQIYTKLKNAGRRLAKCSEITFHTTKTLPMQIDGEPWMQTPCTIKITHKNQMPMLMGPPPRSTNFFGFLS

Target ORF information:

RefSeq Version	XM_017018902.1
Organism	Homo sapiens(human)
Definition	Homo sapiens diacylglycerol kinase alpha (DGKA), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017018902.1

ORF Insert Sequence:

ATGGCCAAGG AGAGGGGCCT AATAAGCCCC AGTGATTTTG CCCAGCTGCA AAAATACATG 
GAATACTCCA CCAAAAAGGT CAGTGATGTC CTAAAGCTCT TCGAGGATGG CGAGATGGCT 
AAATATGTCC AAGGAGATGC CATTGGGTAC GAGGGATTCC AGCAATTCCT GAAAATCTAT 
CTCGAAGTGG ATAATGTTCC CAGACACCTA AGCCTGGCAC TGTTTCAATC CTTTGAGACT 
GGTCACTGCT TAAATGAGAC AAATGTGACA AAAGATGTGG TGTGTCTCAA TGATGTTTCC 
TGCTACTTTT CCCTTCTGGA GGGTGGTCGG CCAGAAGACA AGTTAGAATT CACCTTCAAG 
CTGTACGACA CGGACAGAAA TGGGATCCTG GACAGCTCAG TGAGTTGGGG ACCCTGTATG 
CTGGGCAAGG GAATATGTGT TAATTTGTCT GCACCCTCCT GCCCCAACTC TTCCAGGGTC 
TTAAGAAACA GAAGAGGATG GGGGGAGAGG TTGAGTCCTC TAGGACTAAA TTTGAAAGTA 
AGTCCCAAGC TCTTGACCCC TCAAAGAGAA TGCTGGATAC ATGAATTGGG TCTGCCTTAC 
AAACATACAA ACACATTTAT TTACTTGGCA GACATACCTG AGAATGAGAG GTGTGAATCT 
GGGGTGGAAC CCAGGAACAC TGATTTCTGG AAAAGAGTTT GGATGGTGGT AGTAAAGGGG 
ATATGGCTGT GGCTCTTGCC CTTTTTGCTC CAGGAAGTGG ACAAAATTAT CCTACAGATG 
ATGCGAGTGG CTGAATACCT GGATTGGGAT GTGTCTGAGC TGAGGCCGAT TCTTCAGGAG 
ATGATGAAAG AGATTGACTA TGATGGCAGT GGCTCTGTCT CTCAAGCTGA GTGGGTCCGG 
GCTGGGGCCA CCACCGTGCC ACTGCTAGTG CTGCTGGGTC TGGAGATGAC TCTGAAGGAC 
GACGGACAGC ACATGTGGAG GCCCAAGAGG TTCCCCAGAC CAGTCTACTG CAATCTGTGC 
GAGTCAAGCA TTGGTCTTGG CAAACAGGGA CTGAGCTGTA ACCTCTGTAA GTACACTGTT 
CACGACCAGT GTGCCATGAA AGCCCTGCCT TGTGAAGTCA GCACCTATGC CAAGTCTCGG 
AAGGACATTG GTGTCCAATC ACATGTGTGG GTGCGAGGAG GCTGTGAGTC CGGGCGCTGC 
GACCGCTGTC AGAAAAAGAT CCGGATCTAC CACAGTCTGA CCGGGCTGCA TTGTGTATGG 
TGCCACCTAG AGATCCACGA TGACTGCCTG CAAGCGGTGG GCCATGAGTG TGACTGTGGG 
CTGCTCCGGG ATCACATCCT GCCTCCATCT TCCATCTATC CCAGTGTCCT GGCCTCTGGA 
CCGGATCGTA AAAATAGCAA AACAAGCCAG AAGACCATGG ATGATTTAAA TTTGAGCACC 
TCTGAGGCTC TGCGGATTGA CCCTGTTCCT AACACCCACC CACTTCTCGT CTTTGTCAAT 
CCTAAGAGTG GCGGGAAGCA GGGGCAAAGG GTGCTCTGGA AGTTCCAGTA TATATTAAAC 
CCTCGACAGG TGTTCAACCT CCTAAAGGAT GGTCCTGAGA TAGGGCTCCG ATTATTCAAG 
GATGTTCCTG ATAGCCGGAT TTTGGTGTGT GGTGGAGACG GCACAGTAGG CTGGATTCTA 
GAGACCATTG ACAAAGCTAA CTTGCCAGTT TTGCCTCCTG TTGCTGTGTT GCCCCTGGGT 
ACTGGAAATG ATCTGGCTCG ATGCCTAAGA TGGGGAGGAG GTTATGAAGG ACAGAATCTG 
GCAAAGATCC TCAAGGATTT AGAGATGAGT AAAGTGGTAC ATATGGATCG ATGGTCTGTG 
GAGGTGATAC CTCAACAAAC TGAAGAAAAA AGTGACCCAG TCCCCTTTCA AATCATCAAT 
AACTACTTCT CTATTGGCGT GGATGCCTCT ATTGCTCATC GATTCCACAT CATGCGAGAG 
AAATATCCGG AGAAGTTCAA CAGCAGAATG AAGAACAAGC TATGGTACTT CGAATTTGCC 
ACATCTGAAT CCATCTTCTC AACATGCAAA AAGCTGGAGG AGTCTTTGAC AGTTGAGATC 
TGTGGGAAAC CGCTGGATCT GAGCAACCTG TCCCTAGAAG GCATCGCAGT GCTAAACATC 
CCTAGCATGC ATGGTGGCTC CAACCTCTGG GGTGATACCA GGAGACCCCA TGGGGATATC 
TATGGGATCA ACCAGGCCTT AGGTGCTACA GCTAAAGTCA TCACCGACCC TGATATCCTG 
AAAACCTGTG TACCAGACCT AAGTGACAAG AGACTGGAAG TGGTTGGGCT GGAGGGTGCA 
ATTGAGATGG GCCAAATCTA TACCAAGCTC AAGAATGCTG GACGTCGGCT GGCCAAGTGC 
TCTGAGATCA CCTTCCACAC CACAAAAACC CTTCCCATGC AAATTGACGG AGAACCCTGG 
ATGCAGACGC CCTGTACAAT CAAGATCACC CACAAGAACC AGATGCCCAT GCTCATGGGC 
CCACCCCCCC GCTCCACCAA TTTCTTTGGC TTCTTGAGCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DGKA ( XM_017018908.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016874397.1 Homo sapiens diacylglycerol kinase alpha (DGKA), transcript variant X9, mRNA.

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CloneID	OHu83569
Clone ID Related Accession (Same CDS sequence)	XM_017018908.1
Accession Version	XM_017018908.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2460bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	diacylglycerol kinase alpha isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGTCAT GGCCAGGAAG ACGCGCTAAC GCAGCCTACC CTCTGAAGAG GTCCAAGCAA 
CGGAAGTACT ACTACGAAGC TGCCTTTCTG GCCATCCTTG AGAAAAATAG ACAGATGGCC 
AAGGAGAGGG GCCTAATAAG CCCCAGTGAT TTTGCCCAGC TGCAAAAATA CATGGAATAC 
TCCACCAAAA AGGTCAGTGA TGTCCTAAAG CTCTTCGAGG ATGGCGAGAT GGCTAAATAT 
GTCCAAGGAG ATGCCATTGG GTACGAGGGA TTCCAGCAAT TCCTGAAAAT CTATCTCGAA 
GTGGATAATG TTCCCAGACA CCTAAGCCTG GCACTGTTTC AATCCTTTGA GACTGGTCAC 
TGCTTAAATG AGACAAATGT GACAAAAGAT GTGGTGTGTC TCAATGATGT TTCCTGCTAC 
TTTTCCCTTC TGGAGGGTGG TCGGCCAGAA GACAAGTTAG AATTCACCTT CAAGCTGTAC 
GACACGGACA GAAATGGGAT CCTGGACAGC TCAGTGAGTT GGGGACCCTG TATGCTGGGC 
AAGGGAATAT GTGTTAATTT GTCTGCACCC TCCTGCCCCA ACTCTTCCAG GGTCTTAAGA 
AACAGAAGAG GATGGGGGGA GAGGTTGAGT CCTCTAGGAC TAAATTTGAA AGAAGTGGAC 
AAAATTATCC TACAGATGAT GCGAGTGGCT GAATACCTGG ATTGGGATGT GTCTGAGCTG 
AGGCCGATTC TTCAGGAGAT GATGAAAGAG ATTGACTATG ATGGCAGTGG CTCTGTCTCT 
CAAGCTGAGT GGGTCCGGGC TGGGGCCACC ACCGTGCCAC TGCTAGTGCT GCTGGGTCTG 
GAGATGACTC TGAAGGACGA CGGACAGCAC ATGTGGAGGC CCAAGAGGTT CCCCAGACCA 
GTCTACTGCA ATCTGTGCGA GTCAAGCATT GGTCTTGGCA AACAGGGACT GAGCTGTAAC 
CTCTGTAAGT ACACTGTTCA CGACCAGTGT GCCATGAAAG CCCTGCCTTG TGAAGTCAGC 
ACCTATGCCA AGTCTCGGAA GGACATTGGT GTCCAATCAC ATGTGTGGGT GCGAGGAGGC 
TGTGAGTCCG GGCGCTGCGA CCGCTGTCAG AAAAAGATCC GGATCTACCA CAGTCTGACC 
GGGCTGCATT GTGTATGGTG CCACCTAGAG ATCCACGATG ACTGCCTGCA AGCGGTGGGC 
CATGAGTGTG ACTGTGGGCT GCTCCGGGAT CACATCCTGC CTCCATCTTC CATCTATCCC 
AGTGTCCTGG CCTCTGGACC GGATCGTAAA AATAGCAAAA CAAGCCAGAA GACCATGGAT 
GATTTAAATT TGAGCACCTC TGAGGCTCTG CGGATTGACC CTGTTCCTAA CACCCACCCA 
CTTCTCGTCT TTGTCAATCC TAAGAGTGGC GGGAAGCAGG GGCAAAGGGT GCTCTGGAAG 
TTCCAGTATA TATTAAACCC TCGACAGGTG TTCAACCTCC TAAAGGATGG TCCTGAGATA 
GGGCTCCGAT TATTCAAGGA TGTTCCTGAT AGCCGGATTT TGGTGTGTGG TGGAGACGGC 
ACAGTAGGCT GGATTCTAGA GACCATTGAC AAAGCTAACT TGCCAGTTTT GCCTCCTGTT 
GCTGTGTTGC CCCTGGGTAC TGGAAATGAT CTGGCTCGAT GCCTAAGATG GGGAGGAGGT 
TATGAAGGAC AGAATCTGGC AAAGATCCTC AAGGATTTAG AGATGAGTAA AGTGGTACAT 
ATGGATCGAT GGTCTGTGGA GGTGATACCT CAACAAACTG AAGAAAAAAG TGACCCAGTC 
CCCTTTCAAA TCATCAATAA CTACTTCTCT ATTGGCGTGG ATGCCTCTAT TGCTCATCGA 
TTCCACATCA TGCGAGAGAA ATATCCGGAG AAGTTCAACA GCAGAATGAA GAACAAGCTA 
TGGTACTTCG AATTTGCCAC ATCTGAATCC ATCTTCTCAA CATGCAAAAA GCTGGAGGAG 
TCTTTGACAG TTGAGATCTG TGGGAAACCG CTGGATCTGA GCAACCTGTC CCTAGAAGGC 
ATCGCAGTGC TAAACATCCC TAGCATGCAT GGTGGCTCCA ACCTCTGGGG TGATACCAGG 
AGACCCCATG GGGATATCTA TGGGATCAAC CAGGCCTTAG GTGCTACAGC TAAAGTCATC 
ACCGACCCTG ATATCCTGAA AACCTGTGTA CCAGACCTAA GTGACAAGAG ACTGGAAGTG 
GTTGGGCTGG AGGGTGCAAT TGAGATGGGC CAAATCTATA CCAAGCTCAA GAATGCTGGA 
CGTCGGCTGG CCAAGTGCTC TGAGATCACC TTCCACACCA CAAAAACCCT TCCCATGCAA 
ATTGACGGAG AACCCTGGAT GCAGACGCCC TGTACAATCA AGATCACCCA CAAGAACCAG 
ATGCCCATGC TCATGGGCCC ACCCCCCCGC TCCACCAATT TCTTTGGCTT CTTGAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016874397.1
CDS	93..2552
Translation	MGSWPGRRANAAYPLKRSKQRKYYYEAAFLAILEKNRQMAKERGLISPSDFAQLQKYMEYSTKKVSDVLKLFEDGEMAKYVQGDAIGYEGFQQFLKIYLEVDNVPRHLSLALFQSFETGHCLNETNVTKDVVCLNDVSCYFSLLEGGRPEDKLEFTFKLYDTDRNGILDSSVSWGPCMLGKGICVNLSAPSCPNSSRVLRNRRGWGERLSPLGLNLKEVDKIILQMMRVAEYLDWDVSELRPILQEMMKEIDYDGSGSVSQAEWVRAGATTVPLLVLLGLEMTLKDDGQHMWRPKRFPRPVYCNLCESSIGLGKQGLSCNLCKYTVHDQCAMKALPCEVSTYAKSRKDIGVQSHVWVRGGCESGRCDRCQKKIRIYHSLTGLHCVWCHLEIHDDCLQAVGHECDCGLLRDHILPPSSIYPSVLASGPDRKNSKTSQKTMDDLNLSTSEALRIDPVPNTHPLLVFVNPKSGGKQGQRVLWKFQYILNPRQVFNLLKDGPEIGLRLFKDVPDSRILVCGGDGTVGWILETIDKANLPVLPPVAVLPLGTGNDLARCLRWGGGYEGQNLAKILKDLEMSKVVHMDRWSVEVIPQQTEEKSDPVPFQIINNYFSIGVDASIAHRFHIMREKYPEKFNSRMKNKLWYFEFATSESIFSTCKKLEESLTVEICGKPLDLSNLSLEGIAVLNIPSMHGGSNLWGDTRRPHGDIYGINQALGATAKVITDPDILKTCVPDLSDKRLEVVGLEGAIEMGQIYTKLKNAGRRLAKCSEITFHTTKTLPMQIDGEPWMQTPCTIKITHKNQMPMLMGPPPRSTNFFGFLS

Target ORF information:

RefSeq Version	XM_017018908.1
Organism	Homo sapiens(human)
Definition	Homo sapiens diacylglycerol kinase alpha (DGKA), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017018908.1

ORF Insert Sequence:

ATGGGGTCAT GGCCAGGAAG ACGCGCTAAC GCAGCCTACC CTCTGAAGAG GTCCAAGCAA 
CGGAAGTACT ACTACGAAGC TGCCTTTCTG GCCATCCTTG AGAAAAATAG ACAGATGGCC 
AAGGAGAGGG GCCTAATAAG CCCCAGTGAT TTTGCCCAGC TGCAAAAATA CATGGAATAC 
TCCACCAAAA AGGTCAGTGA TGTCCTAAAG CTCTTCGAGG ATGGCGAGAT GGCTAAATAT 
GTCCAAGGAG ATGCCATTGG GTACGAGGGA TTCCAGCAAT TCCTGAAAAT CTATCTCGAA 
GTGGATAATG TTCCCAGACA CCTAAGCCTG GCACTGTTTC AATCCTTTGA GACTGGTCAC 
TGCTTAAATG AGACAAATGT GACAAAAGAT GTGGTGTGTC TCAATGATGT TTCCTGCTAC 
TTTTCCCTTC TGGAGGGTGG TCGGCCAGAA GACAAGTTAG AATTCACCTT CAAGCTGTAC 
GACACGGACA GAAATGGGAT CCTGGACAGC TCAGTGAGTT GGGGACCCTG TATGCTGGGC 
AAGGGAATAT GTGTTAATTT GTCTGCACCC TCCTGCCCCA ACTCTTCCAG GGTCTTAAGA 
AACAGAAGAG GATGGGGGGA GAGGTTGAGT CCTCTAGGAC TAAATTTGAA AGAAGTGGAC 
AAAATTATCC TACAGATGAT GCGAGTGGCT GAATACCTGG ATTGGGATGT GTCTGAGCTG 
AGGCCGATTC TTCAGGAGAT GATGAAAGAG ATTGACTATG ATGGCAGTGG CTCTGTCTCT 
CAAGCTGAGT GGGTCCGGGC TGGGGCCACC ACCGTGCCAC TGCTAGTGCT GCTGGGTCTG 
GAGATGACTC TGAAGGACGA CGGACAGCAC ATGTGGAGGC CCAAGAGGTT CCCCAGACCA 
GTCTACTGCA ATCTGTGCGA GTCAAGCATT GGTCTTGGCA AACAGGGACT GAGCTGTAAC 
CTCTGTAAGT ACACTGTTCA CGACCAGTGT GCCATGAAAG CCCTGCCTTG TGAAGTCAGC 
ACCTATGCCA AGTCTCGGAA GGACATTGGT GTCCAATCAC ATGTGTGGGT GCGAGGAGGC 
TGTGAGTCCG GGCGCTGCGA CCGCTGTCAG AAAAAGATCC GGATCTACCA CAGTCTGACC 
GGGCTGCATT GTGTATGGTG CCACCTAGAG ATCCACGATG ACTGCCTGCA AGCGGTGGGC 
CATGAGTGTG ACTGTGGGCT GCTCCGGGAT CACATCCTGC CTCCATCTTC CATCTATCCC 
AGTGTCCTGG CCTCTGGACC GGATCGTAAA AATAGCAAAA CAAGCCAGAA GACCATGGAT 
GATTTAAATT TGAGCACCTC TGAGGCTCTG CGGATTGACC CTGTTCCTAA CACCCACCCA 
CTTCTCGTCT TTGTCAATCC TAAGAGTGGC GGGAAGCAGG GGCAAAGGGT GCTCTGGAAG 
TTCCAGTATA TATTAAACCC TCGACAGGTG TTCAACCTCC TAAAGGATGG TCCTGAGATA 
GGGCTCCGAT TATTCAAGGA TGTTCCTGAT AGCCGGATTT TGGTGTGTGG TGGAGACGGC 
ACAGTAGGCT GGATTCTAGA GACCATTGAC AAAGCTAACT TGCCAGTTTT GCCTCCTGTT 
GCTGTGTTGC CCCTGGGTAC TGGAAATGAT CTGGCTCGAT GCCTAAGATG GGGAGGAGGT 
TATGAAGGAC AGAATCTGGC AAAGATCCTC AAGGATTTAG AGATGAGTAA AGTGGTACAT 
ATGGATCGAT GGTCTGTGGA GGTGATACCT CAACAAACTG AAGAAAAAAG TGACCCAGTC 
CCCTTTCAAA TCATCAATAA CTACTTCTCT ATTGGCGTGG ATGCCTCTAT TGCTCATCGA 
TTCCACATCA TGCGAGAGAA ATATCCGGAG AAGTTCAACA GCAGAATGAA GAACAAGCTA 
TGGTACTTCG AATTTGCCAC ATCTGAATCC ATCTTCTCAA CATGCAAAAA GCTGGAGGAG 
TCTTTGACAG TTGAGATCTG TGGGAAACCG CTGGATCTGA GCAACCTGTC CCTAGAAGGC 
ATCGCAGTGC TAAACATCCC TAGCATGCAT GGTGGCTCCA ACCTCTGGGG TGATACCAGG 
AGACCCCATG GGGATATCTA TGGGATCAAC CAGGCCTTAG GTGCTACAGC TAAAGTCATC 
ACCGACCCTG ATATCCTGAA AACCTGTGTA CCAGACCTAA GTGACAAGAG ACTGGAAGTG 
GTTGGGCTGG AGGGTGCAAT TGAGATGGGC CAAATCTATA CCAAGCTCAA GAATGCTGGA 
CGTCGGCTGG CCAAGTGCTC TGAGATCACC TTCCACACCA CAAAAACCCT TCCCATGCAA 
ATTGACGGAG AACCCTGGAT GCAGACGCCC TGTACAATCA AGATCACCCA CAAGAACCAG 
ATGCCCATGC TCATGGGCCC ACCCCCCCGC TCCACCAATT TCTTTGGCTT CTTGAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Nuclear Localization of Diacylglycerol Kinase Alpha in K562 Cells Is Involved in Cell Cycle Progression.
Journal of cellular physiology232(9)2550-2557(2017 Sep)
Poli A,Fiume R,Baldanzi G,Capello D,Ratti S,Gesi M,Manzoli L,Graziani A,Suh PG,Cocco L,Follo MY

Diacylglycerol kinases in cancer.
Advances in biological regulation6322-31(2017 Jan)
M?rida I,Torres-Ayuso P,?vila-Flores A,Arranz-Nicol?s J,Andrada E,Tello-Lafoz M,Li?bana R,Arcos R

Decreased LIPF expression is correlated with DGKA and predicts poor outcome of gastric cancer.
Oncology reports36(4)1852-60(2016 Oct)
Kong Y,Zheng Y,Jia Y,Li P,Wang Y

Epigenetic regulation of diacylglycerol kinase alpha promotes radiation-induced fibrosis.
Nature communications710893(2016 Mar)
Weigel C,Veldwijk MR,Oakes CC,Seibold P,Slynko A,Liesenfeld DB,Rabionet M,Hanke SA,Wenz F,Sperk E,Benner A,R?sli C,Sandhoff R,Assenov Y,Plass C,Herskind C,Chang-Claude J,Schmezer P,Popanda O

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DGKA cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

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