AARS cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	AARS
Entrez Gene ID	16
Full Name	alanyl-tRNA synthetase
Synonyms	CMT2N,EIEE29
General protein information	Preferred Names alanyl-tRNA synthetase Names alanine--tRNA ligase, cytoplasmic alaRS alanine tRNA ligase 1, cytoplasmic alanyl-tRNA synthetase, cytoplasmic renal carcinoma antigen NY-REN-42
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 16 Map Location: 16q22.1
Summary	The human alanyl-tRNA synthetase (AARS) belongs to a family of tRNA synthases, of the class II enzymes. Class II tRNA synthases evolved early in evolution and are highly conserved. This is reflected by the fact that 498 of the 968-residue polypeptide human AARS shares 41% identity witht the E.coli protein. tRNA synthases are the enzymes that interpret the RNA code and attach specific aminoacids to the tRNAs that contain the cognate trinucleotide anticodons. They consist of a catalytic domain which interacts with the amino acid acceptor-T psi C helix of the tRNA, and a second domain which interacts with the rest of the tRNA structure. [provided by RefSeq, Jul 2008].
Disorder MIM:	601065
Disorder Html:	Charcot-Marie-Tooth disease, axonal, type 2N, 613287 (3); Epileptic encephalopathy, early infantile, 29, 616339 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001605.3	NP_001596.2	alanine--tRNA ligase, cytoplasmic
NM_001605.2	NP_001596.2	alanine--tRNA ligase, cytoplasmic

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	AARS	XP_001169520.2
Caenorhabditis elegans (roundworm)	aars-2	NP_491281.1
Neurospora crassa	NCU02566	XP_965697.2
Xenopus tropicalis (tropical clawed frog)	aars	XP_002931707.1
	AARS	XP_002808351.1
Mus musculus (house mouse)	Aars	NP_666329.2
Danio rerio (zebrafish)	aars	NP_001037775.1
Oryza sativa (rice)	Os10g0182000	NP_001064254.1
Canis lupus familiaris (dog)	AARS	XP_536788.2
Gallus gallus (chicken)	AARS	NP_001005836.1
Drosophila melanogaster (fruit fly)	Aats-ala	NP_523511.2
Kluyveromyces lactis	KLLA0F02431g	XP_455190.1
Schizosaccharomyces pombe (fission yeast)	ala1	NP_593640.1
Homo sapiens (human)	AARS	NP_001596.2
Bos taurus (cattle)	AARS	NP_001094545.1
Rattus norvegicus (Norway rat)	Aars	NP_001093987.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009701	XP_318757.4
Saccharomyces cerevisiae (baker's yeast)	ALA1	NP_014980.3
Eremothecium gossypii	AGOS_ADR363C	NP_984459.1
Magnaporthe oryzae (rice blast fungus)	MGG_03607	XP_003716291.1
Arabidopsis thaliana (thale cress)	ALATS	NP_001185186.1

Gene Ontology
Bibliography

Related articles in PubMed

Deficient activity of alanyl-tRNA synthetase underlies an autosomal recessive syndrome of progressive microcephaly, hypomyelination, and epileptic encephalopathy.
Nakayama T, Wu J, Galvin-Parton P, Weiss J, Andriola MR, Hill RS, Vaughan DJ, El-Quessny M, Barry BJ, Partlow JN, Barkovich AJ, Ling J, Mochida GH
Human mutation38(10)1348-1354(2017 Oct)

Two crystal structures reveal design for repurposing the C-Ala domain of human AlaRS.
Sun L, Song Y, Blocquel D, Yang XL, Schimmel P
Proceedings of the National Academy of Sciences of the United States of America113(50)14300-14305(2016 Dec)

Evolutionary Gain of Alanine Mischarging to Noncognate tRNAs with a G4:U69 Base Pair.
Sun L, Gomes AC, He W, Zhou H, Wang X, Pan DW, Schimmel P, Pan T, Yang XL
Journal of the American Chemical Society138(39)12948-12955(2016 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Number of missense mutations in AARS expand the clinical spectrum and provide pheno-genotypic correlations in AARS-related neuropathies.
Title: Genotype/phenotype correlations in AARS-related neuropathy in a cohort of patients from the United Kingdom and Ireland.

A novel mutation in alanyl-tRNA synthetase causes a mild myeloneuropathy, a novel phenotype for patients with mutations in one of the tRNA synthetase genes.
Title: A novel AARS mutation in a family with dominant myeloneuropathy.

Loss-of-function alanyl-tRNA synthetase mutations cause an autosomal-recessive early-onset epileptic encephalopathy with persistent myelination defect.
Title: Loss-of-function alanyl-tRNA synthetase mutations cause an autosomal-recessive early-onset epileptic encephalopathy with persistent myelination defect.

the pathological consequences of diminished tRNA synthetase editing activity, and thus translational infidelity, are dependent on the cell type and the extent of editing disruption
Title: Deficiencies in tRNA synthetase editing activity cause cardioproteinopathy.

in a family with distal hereditary motor neuropathy (dHMN), all 4 affected family members had a heterozygous missense mutation c.2677G>A (p.D893N) of (AARS), not found in the 4 unaffected members and control subjects; conclude AARS mutation caused dHMN in a Chinese family; AARS mutations result in not only a CMT phenotype but also a dHMN phenotype
Title: Alanyl-tRNA synthetase mutation in a family with dominant distal hereditary motor neuropathy.

The following AARS gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AARS cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu25931	NM_001605.3 Latest version!	Homo sapiens alanyl-tRNA synthetase 1 (AARS1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
View Old Accession Versions >>
OHu25931	NM_001605.2	Homo sapiens alanyl-tRNA synthetase (AARS), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

AARS ( NM_001605.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001596.2 Homo sapiens alanyl-tRNA synthetase (AARS), mRNA.

Price & Availability↑

CloneID	OHu25931
Clone ID Related Accession (Same CDS sequence)	NM_001605.3 , NM_001605.2
Accession Version	NM_001605.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2907bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-05
Organism	Homo sapiens(human)
Product	alanine--tRNA ligase, cytoplasmic
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from D32050.1, BC011451.1, BG764123.1 and BU178772.1. This sequence is a reference standard in the RefSeqGene project. On Jun 15, 2006 this sequence version replaced NM_001605.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: D32050.1, SRR1660805.154308.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGACTCTA CTCTAACAGC AAGTGAAATC CGGCAGCGAT TTATAGATTT CTTCAAGAGG 
AACGAGCATA CGTATGTTCA CTCGTCTGCC ACCATCCCAT TGGATGACCC CACTTTGCTC 
TTTGCCAATG CAGGCATGAA CCAGTTTAAA CCCATTTTCC TGAACACAAT TGACCCATCT 
CACCCCATGG CAAAGCTGAG CAGAGCTGCC AATACCCAGA AGTGCATCCG GGCTGGGGGC 
AAACATAATG ACCTGGACGA TGTGGGCAAG GATGTCTATC ATCACACCTT CTTCGAGATG 
CTGGGCTCTT GGTCTTTTGG AGATTACTTT AAGGAATTGG CATGTAAGAT GGCTCTGGAA 
CTCCTCACCC AAGAGTTTGG CATTCCCATT GAAAGACTTT ATGTTACTTA CTTTGGCGGG 
GATGAAGCAG CTGGCTTAGA AGCAGATCTG GAATGCAAAC AGATCTGGCA AAATTTGGGG 
CTGGATGACA CCAAAATCCT CCCAGGCAAC ATGAAGGATA ACTTCTGGGA GATGGGTGAC 
ACGGGCCCCT GTGGTCCTTG CAGTGAGATC CACTACGACC GGATTGGTGG TCGGGACGCC 
GCACATCTTG TCAACCAGGA CGACCCTAAT GTGCTGGAGA TCTGGAACCT TGTGTTCATC 
CAGTATAACA GGGAAGCTGA TGGCATTCTG AAACCTCTTC CCAAGAAAAG CATTGACACA 
GGGATGGGCC TGGAACGACT GGTATCTGTG CTGCAGAATA AGATGTCCAA CTATGACACT 
GACCTTTTTG TCCCTTACTT TGAAGCCATT CAGAAGGGCA CAGGTGCCCG ACCATACACT 
GGGAAAGTTG GTGCTGAGGA TGCCGATGGG ATTGACATGG CCTACCGGGT GCTGGCTGAC 
CACGCTCGGA CCATCACTGT GGCACTGGCT GATGGTGGCC GGCCTGACAA CACAGGGCGT 
GGATATGTGT TGAGACGGAT TCTCCGCCGA GCTGTCCGAT ACGCCCATGA AAAGCTCAAT 
GCCAGCAGGG GCTTCTTTGC TACGTTAGTG GATGTTGTCG TCCAGTCCCT GGGAGATGCA 
TTTCCTGAGC TGAAGAAGGA CCCAGACATG GTGAAGGACA TCATTAATGA AGAAGAGGTG 
CAGTTTCTCA AGACTCTCAG CAGAGGGCGT CGCATCCTGG ACAGGAAAAT TCAGAGCCTG 
GGAGACAGCA AGACCATTCC CGGAGACACT GCTTGGCTCC TCTATGACAC CTATGGGTTT 
CCAGTGGATC TGACTGGACT GATTGCTGAA GAGAAGGGCC TGGTGGTAGA CATGGATGGC 
TTTGAAGAGG AGAGGAAACT GGCCCAGCTG AAATCACAGG GCAAGGGAGC TGGTGGGGAA 
GACCTCATTA TGCTGGACAT TTACGCTATC GAAGAGCTCC GGGCACGGGG TCTGGAGGTC 
ACAGATGATT CCCCAAAGTA CAATTACCAT TTGGACTCCA GTGGTAGCTA TGTATTTGAG 
AACACAGTGG CTACGGTGAT GGCTCTGCGC AGGGAGAAGA TGTTCGTGGA AGAGGTGTCC 
ACAGGCCAGG AGTGTGGAGT GGTGCTGGAC AAGACCTGTT TCTATGCTGA GCAAGGAGGC 
CAGATCTATG ACGAAGGCTA CCTGGTGAAG GTGGATGACA GCAGTGAAGA TAAAACAGAG 
TTTACAGTGA AGAATGCTCA GGTCCGAGGA GGGTATGTGC TACACATTGG AACCATCTAC 
GGTGACCTGA AAGTGGGGGA TCAGGTCTGG CTGTTTATTG ATGAGCCCCG ACGAAGACCC 
ATCATGAGCA ACCACACAGC TACGCACATT CTGAACTTCG CCCTGCGCTC AGTGCTTGGG 
GAAGCTGACC AGAAAGGCTC ATTGGTTGCT CCTGACCGCC TCAGATTTGA CTTTACTGCC 
AAGGGAGCCA TGTCCACCCA ACAGATCAAG AAGGCTGAAG AGATTGCTAA TGAGATGATT 
GAGGCAGCCA AGGCCGTCTA TACCCAGGAT TGCCCCCTGG CAGCAGCGAA AGCCATCCAG 
GGCCTACGGG CTGTGTTTGA TGAGACCTAT CCTGACCCTG TGCGAGTCGT CTCCATTGGG 
GTCCCGGTGT CCGAGTTGCT GGATGACCCC TCTGGGCCTG CTGGCTCCCT GACTTCTGTT 
GAGTTCTGTG GGGGAACGCA CCTGCGGAAC TCGAGTCATG CAGGAGCTTT TGTGATCGTG 
ACGGAAGAAG CCATTGCCAA GGGTATCCGG AGGATTGTGG CTGTCACAGG TGCCGAGGCC 
CAGAAGGCCC TCAGGAAAGC AGAGAGCTTG AAGAAATGTC TCTCTGTCAT GGAAGCCAAA 
GTGAAGGCTC AGACTGCTCC AAACAAGGAT GTGCAGAGGG AGATCGCTGA CCTTGGAGAG 
GCCCTGGCCA CTGCAGTCAT CCCCCAGTGG CAGAAGGATG AATTGCGGGA GACTCTCAAA 
TCCCTAAAGA AGGTCATGGA TGACTTGGAC CGAGCCAGCA AAGCCGATGT CCAGAAACGA 
GTGTTAGAGA AGACGAAGCA GTTCATCGAC AGCAACCCCA ACCAGCCTCT TGTCATCCTG 
GAGATGGAGA GCGGCGCCTC AGCCAAGGCC CTGAATGAAG CCTTGAAGCT CTTCAAGATG 
CACTCCCCTC AGACTTCTGC CATGCTCTTC ACGGTGGACA ATGAGGCTGG CAAGATCACG 
TGCCTGTGTC AAGTTCCCCA GAATGCAGCC AATCGGGGCT TAAAAGCCAG CGAGTGGGTG 
CAGCAGGTGT CAGGCTTGAT GGACGGTAAA GGTGGTGGCA AGGATGTGTC TGCACAGGCC 
ACAGGCAAGA ACGTTGGCTG CCTGCAGGAG GCGCTGCAGC TGGCCACTTC CTTCGCCCAG 
CTGCGCCTCG GGGATGTAAA GAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001596.2
CDS	111..3017
Translation	MDSTLTASEIRQRFIDFFKRNEHTYVHSSATIPLDDPTLLFANAGMNQFKPIFLNTIDPSHPMAKLSRAANTQKCIRAGGKHNDLDDVGKDVYHHTFFEMLGSWSFGDYFKELACKMALELLTQEFGIPIERLYVTYFGGDEAAGLEADLECKQIWQNLGLDDTKILPGNMKDNFWEMGDTGPCGPCSEIHYDRIGGRDAAHLVNQDDPNVLEIWNLVFIQYNREADGILKPLPKKSIDTGMGLERLVSVLQNKMSNYDTDLFVPYFEAIQKGTGARPYTGKVGAEDADGIDMAYRVLADHARTITVALADGGRPDNTGRGYVLRRILRRAVRYAHEKLNASRGFFATLVDVVVQSLGDAFPELKKDPDMVKDIINEEEVQFLKTLSRGRRILDRKIQSLGDSKTIPGDTAWLLYDTYGFPVDLTGLIAEEKGLVVDMDGFEEERKLAQLKSQGKGAGGEDLIMLDIYAIEELRARGLEVTDDSPKYNYHLDSSGSYVFENTVATVMALRREKMFVEEVSTGQECGVVLDKTCFYAEQGGQIYDEGYLVKVDDSSEDKTEFTVKNAQVRGGYVLHIGTIYGDLKVGDQVWLFIDEPRRRPIMSNHTATHILNFALRSVLGEADQKGSLVAPDRLRFDFTAKGAMSTQQIKKAEEIANEMIEAAKAVYTQDCPLAAAKAIQGLRAVFDETYPDPVRVVSIGVPVSELLDDPSGPAGSLTSVEFCGGTHLRNSSHAGAFVIVTEEAIAKGIRRIVAVTGAEAQKALRKAESLKKCLSVMEAKVKAQTAPNKDVQREIADLGEALATAVIPQWQKDELRETLKSLKKVMDDLDRASKADVQKRVLEKTKQFIDSNPNQPLVILEMESGASAKALNEALKLFKMHSPQTSAMLFTVDNEAGKITCLCQVPQNAANRGLKASEWVQQVSGLMDGKGGGKDVSAQATGKNVGCLQEALQLATSFAQLRLGDVKN

Target ORF information:

RefSeq Version	NM_001605.2
Organism	Homo sapiens(human)
Definition	Homo sapiens alanyl-tRNA synthetase (AARS), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001605.2

ORF Insert Sequence:

ATGGACTCTA CTCTAACAGC AAGTGAAATC CGGCAGCGAT TTATAGATTT CTTCAAGAGG 
AACGAGCATA CGTATGTTCA CTCGTCTGCC ACCATCCCAT TGGATGACCC CACTTTGCTC 
TTTGCCAATG CAGGCATGAA CCAGTTTAAA CCCATTTTCC TGAACACAAT TGACCCATCT 
CACCCCATGG CAAAGCTGAG CAGAGCTGCC AATACCCAGA AGTGCATCCG GGCTGGGGGC 
AAACATAATG ACCTGGACGA TGTGGGCAAG GATGTCTATC ATCACACCTT CTTCGAGATG 
CTGGGCTCTT GGTCTTTTGG AGATTACTTT AAGGAATTGG CATGTAAGAT GGCTCTGGAA 
CTCCTCACCC AAGAGTTTGG CATTCCCATT GAAAGACTTT ATGTTACTTA CTTTGGCGGG 
GATGAAGCAG CTGGCTTAGA AGCAGATCTG GAATGCAAAC AGATCTGGCA AAATTTGGGG 
CTGGATGACA CCAAAATCCT CCCAGGCAAC ATGAAGGATA ACTTCTGGGA GATGGGTGAC 
ACGGGCCCCT GTGGTCCTTG CAGTGAGATC CACTACGACC GGATTGGTGG TCGGGACGCC 
GCACATCTTG TCAACCAGGA CGACCCTAAT GTGCTGGAGA TCTGGAACCT TGTGTTCATC 
CAGTATAACA GGGAAGCTGA TGGCATTCTG AAACCTCTTC CCAAGAAAAG CATTGACACA 
GGGATGGGCC TGGAACGACT GGTATCTGTG CTGCAGAATA AGATGTCCAA CTATGACACT 
GACCTTTTTG TCCCTTACTT TGAAGCCATT CAGAAGGGCA CAGGTGCCCG ACCATACACT 
GGGAAAGTTG GTGCTGAGGA TGCCGATGGG ATTGACATGG CCTACCGGGT GCTGGCTGAC 
CACGCTCGGA CCATCACTGT GGCACTGGCT GATGGTGGCC GGCCTGACAA CACAGGGCGT 
GGATATGTGT TGAGACGGAT TCTCCGCCGA GCTGTCCGAT ACGCCCATGA AAAGCTCAAT 
GCCAGCAGGG GCTTCTTTGC TACGTTAGTG GATGTTGTCG TCCAGTCCCT GGGAGATGCA 
TTTCCTGAGC TGAAGAAGGA CCCAGACATG GTGAAGGACA TCATTAATGA AGAAGAGGTG 
CAGTTTCTCA AGACTCTCAG CAGAGGGCGT CGCATCCTGG ACAGGAAAAT TCAGAGCCTG 
GGAGACAGCA AGACCATTCC CGGAGACACT GCTTGGCTCC TCTATGACAC CTATGGGTTT 
CCAGTGGATC TGACTGGACT GATTGCTGAA GAGAAGGGCC TGGTGGTAGA CATGGATGGC 
TTTGAAGAGG AGAGGAAACT GGCCCAGCTG AAATCACAGG GCAAGGGAGC TGGTGGGGAA 
GACCTCATTA TGCTGGACAT TTACGCTATC GAAGAGCTCC GGGCACGGGG TCTGGAGGTC 
ACAGATGATT CCCCAAAGTA CAATTACCAT TTGGACTCCA GTGGTAGCTA TGTATTTGAG 
AACACAGTGG CTACGGTGAT GGCTCTGCGC AGGGAGAAGA TGTTCGTGGA AGAGGTGTCC 
ACAGGCCAGG AGTGTGGAGT GGTGCTGGAC AAGACCTGTT TCTATGCTGA GCAAGGAGGC 
CAGATCTATG ACGAAGGCTA CCTGGTGAAG GTGGATGACA GCAGTGAAGA TAAAACAGAG 
TTTACAGTGA AGAATGCTCA GGTCCGAGGA GGGTATGTGC TACACATTGG AACCATCTAC 
GGTGACCTGA AAGTGGGGGA TCAGGTCTGG CTGTTTATTG ATGAGCCCCG ACGAAGACCC 
ATCATGAGCA ACCACACAGC TACGCACATT CTGAACTTCG CCCTGCGCTC AGTGCTTGGG 
GAAGCTGACC AGAAAGGCTC ATTGGTTGCT CCTGACCGCC TCAGATTTGA CTTTACTGCC 
AAGGGAGCCA TGTCCACCCA ACAGATCAAG AAGGCTGAAG AGATTGCTAA TGAGATGATT 
GAGGCAGCCA AGGCCGTCTA TACCCAGGAT TGCCCCCTGG CAGCAGCGAA AGCCATCCAG 
GGCCTACGGG CTGTGTTTGA TGAGACCTAT CCTGACCCTG TGCGAGTCGT CTCCATTGGG 
GTCCCGGTGT CCGAGTTGCT GGATGACCCC TCTGGGCCTG CTGGCTCCCT GACTTCTGTT 
GAGTTCTGTG GGGGAACGCA CCTGCGGAAC TCGAGTCATG CAGGAGCTTT TGTGATCGTG 
ACGGAAGAAG CCATTGCCAA GGGTATCCGG AGGATTGTGG CTGTCACAGG TGCCGAGGCC 
CAGAAGGCCC TCAGGAAAGC AGAGAGCTTG AAGAAATGTC TCTCTGTCAT GGAAGCCAAA 
GTGAAGGCTC AGACTGCTCC AAACAAGGAT GTGCAGAGGG AGATCGCTGA CCTTGGAGAG 
GCCCTGGCCA CTGCAGTCAT CCCCCAGTGG CAGAAGGATG AATTGCGGGA GACTCTCAAA 
TCCCTAAAGA AGGTCATGGA TGACTTGGAC CGAGCCAGCA AAGCCGATGT CCAGAAACGA 
GTGTTAGAGA AGACGAAGCA GTTCATCGAC AGCAACCCCA ACCAGCCTCT TGTCATCCTG 
GAGATGGAGA GCGGCGCCTC AGCCAAGGCC CTGAATGAAG CCTTGAAGCT CTTCAAGATG 
CACTCCCCTC AGACTTCTGC CATGCTCTTC ACGGTGGACA ATGAGGCTGG CAAGATCACG 
TGCCTGTGTC AAGTTCCCCA GAATGCAGCC AATCGGGGCT TAAAAGCCAG CGAGTGGGTG 
CAGCAGGTGT CAGGCTTGAT GGACGGTAAA GGTGGTGGCA AGGATGTGTC TGCACAGGCC 
ACAGGCAAGA ACGTTGGCTG CCTGCAGGAG GCGCTGCAGC TGGCCACTTC CTTCGCCCAG 
CTGCGCCTCG GGGATGTAAA GAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AARS ( NM_001605.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001596.2 Homo sapiens alanyl-tRNA synthetase 1 (AARS1), mRNA.

Price & Availability↑

CloneID	OHu25931
Clone ID Related Accession (Same CDS sequence)	NM_001605.3 , NM_001605.2
Accession Version	NM_001605.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2907bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-19
Organism	Homo sapiens(human)
Product	alanine--tRNA ligase, cytoplasmic
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from D32050.1, BC011451.1, BG764123.1, BU178772.1 and AC009060.7. On Nov 21, 2019 this sequence version replaced NM_001605.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK222824.1, D32050.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGACTCTA CTCTAACAGC AAGTGAAATC CGGCAGCGAT TTATAGATTT CTTCAAGAGG 
AACGAGCATA CGTATGTTCA CTCGTCTGCC ACCATCCCAT TGGATGACCC CACTTTGCTC 
TTTGCCAATG CAGGCATGAA CCAGTTTAAA CCCATTTTCC TGAACACAAT TGACCCATCT 
CACCCCATGG CAAAGCTGAG CAGAGCTGCC AATACCCAGA AGTGCATCCG GGCTGGGGGC 
AAACATAATG ACCTGGACGA TGTGGGCAAG GATGTCTATC ATCACACCTT CTTCGAGATG 
CTGGGCTCTT GGTCTTTTGG AGATTACTTT AAGGAATTGG CATGTAAGAT GGCTCTGGAA 
CTCCTCACCC AAGAGTTTGG CATTCCCATT GAAAGACTTT ATGTTACTTA CTTTGGCGGG 
GATGAAGCAG CTGGCTTAGA AGCAGATCTG GAATGCAAAC AGATCTGGCA AAATTTGGGG 
CTGGATGACA CCAAAATCCT CCCAGGCAAC ATGAAGGATA ACTTCTGGGA GATGGGTGAC 
ACGGGCCCCT GTGGTCCTTG CAGTGAGATC CACTACGACC GGATTGGTGG TCGGGACGCC 
GCACATCTTG TCAACCAGGA CGACCCTAAT GTGCTGGAGA TCTGGAACCT TGTGTTCATC 
CAGTATAACA GGGAAGCTGA TGGCATTCTG AAACCTCTTC CCAAGAAAAG CATTGACACA 
GGGATGGGCC TGGAACGACT GGTATCTGTG CTGCAGAATA AGATGTCCAA CTATGACACT 
GACCTTTTTG TCCCTTACTT TGAAGCCATT CAGAAGGGCA CAGGTGCCCG ACCATACACT 
GGGAAAGTTG GTGCTGAGGA TGCCGATGGG ATTGACATGG CCTACCGGGT GCTGGCTGAC 
CACGCTCGGA CCATCACTGT GGCACTGGCT GATGGTGGCC GGCCTGACAA CACAGGGCGT 
GGATATGTGT TGAGACGGAT TCTCCGCCGA GCTGTCCGAT ACGCCCATGA AAAGCTCAAT 
GCCAGCAGGG GCTTCTTTGC TACGTTAGTG GATGTTGTCG TCCAGTCCCT GGGAGATGCA 
TTTCCTGAGC TGAAGAAGGA CCCAGACATG GTGAAGGACA TCATTAATGA AGAAGAGGTG 
CAGTTTCTCA AGACTCTCAG CAGAGGGCGT CGCATCCTGG ACAGGAAAAT TCAGAGCCTG 
GGAGACAGCA AGACCATTCC CGGAGACACT GCTTGGCTCC TCTATGACAC CTATGGGTTT 
CCAGTGGATC TGACTGGACT GATTGCTGAA GAGAAGGGCC TGGTGGTAGA CATGGATGGC 
TTTGAAGAGG AGAGGAAACT GGCCCAGCTG AAATCACAGG GCAAGGGAGC TGGTGGGGAA 
GACCTCATTA TGCTGGACAT TTACGCTATC GAAGAGCTCC GGGCACGGGG TCTGGAGGTC 
ACAGATGATT CCCCAAAGTA CAATTACCAT TTGGACTCCA GTGGTAGCTA TGTATTTGAG 
AACACAGTGG CTACGGTGAT GGCTCTGCGC AGGGAGAAGA TGTTCGTGGA AGAGGTGTCC 
ACAGGCCAGG AGTGTGGAGT GGTGCTGGAC AAGACCTGTT TCTATGCTGA GCAAGGAGGC 
CAGATCTATG ACGAAGGCTA CCTGGTGAAG GTGGATGACA GCAGTGAAGA TAAAACAGAG 
TTTACAGTGA AGAATGCTCA GGTCCGAGGA GGGTATGTGC TACACATTGG AACCATCTAC 
GGTGACCTGA AAGTGGGGGA TCAGGTCTGG CTGTTTATTG ATGAGCCCCG ACGAAGACCC 
ATCATGAGCA ACCACACAGC TACGCACATT CTGAACTTCG CCCTGCGCTC AGTGCTTGGG 
GAAGCTGACC AGAAAGGCTC ATTGGTTGCT CCTGACCGCC TCAGATTTGA CTTTACTGCC 
AAGGGAGCCA TGTCCACCCA ACAGATCAAG AAGGCTGAAG AGATTGCTAA TGAGATGATT 
GAGGCAGCCA AGGCCGTCTA TACCCAGGAT TGCCCCCTGG CAGCAGCGAA AGCCATCCAG 
GGCCTACGGG CTGTGTTTGA TGAGACCTAT CCTGACCCTG TGCGAGTCGT CTCCATTGGG 
GTCCCGGTGT CCGAGTTGCT GGATGACCCC TCTGGGCCTG CTGGCTCCCT GACTTCTGTT 
GAGTTCTGTG GGGGAACGCA CCTGCGGAAC TCGAGTCATG CAGGAGCTTT TGTGATCGTG 
ACGGAAGAAG CCATTGCCAA GGGTATCCGG AGGATTGTGG CTGTCACAGG TGCCGAGGCC 
CAGAAGGCCC TCAGGAAAGC AGAGAGCTTG AAGAAATGTC TCTCTGTCAT GGAAGCCAAA 
GTGAAGGCTC AGACTGCTCC AAACAAGGAT GTGCAGAGGG AGATCGCTGA CCTTGGAGAG 
GCCCTGGCCA CTGCAGTCAT CCCCCAGTGG CAGAAGGATG AATTGCGGGA GACTCTCAAA 
TCCCTAAAGA AGGTCATGGA TGACTTGGAC CGAGCCAGCA AAGCCGATGT CCAGAAACGA 
GTGTTAGAGA AGACGAAGCA GTTCATCGAC AGCAACCCCA ACCAGCCTCT TGTCATCCTG 
GAGATGGAGA GCGGCGCCTC AGCCAAGGCC CTGAATGAAG CCTTGAAGCT CTTCAAGATG 
CACTCCCCTC AGACTTCTGC CATGCTCTTC ACGGTGGACA ATGAGGCTGG CAAGATCACG 
TGCCTGTGTC AAGTTCCCCA GAATGCAGCC AATCGGGGCT TAAAAGCCAG CGAGTGGGTG 
CAGCAGGTGT CAGGCTTGAT GGACGGTAAA GGTGGTGGCA AGGATGTGTC TGCACAGGCC 
ACAGGCAAGA ACGTTGGCTG CCTGCAGGAG GCGCTGCAGC TGGCCACTTC CTTCGCCCAG 
CTGCGCCTCG GGGATGTAAA GAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001596.2
CDS	108..3014
Translation	MDSTLTASEIRQRFIDFFKRNEHTYVHSSATIPLDDPTLLFANAGMNQFKPIFLNTIDPSHPMAKLSRAANTQKCIRAGGKHNDLDDVGKDVYHHTFFEMLGSWSFGDYFKELACKMALELLTQEFGIPIERLYVTYFGGDEAAGLEADLECKQIWQNLGLDDTKILPGNMKDNFWEMGDTGPCGPCSEIHYDRIGGRDAAHLVNQDDPNVLEIWNLVFIQYNREADGILKPLPKKSIDTGMGLERLVSVLQNKMSNYDTDLFVPYFEAIQKGTGARPYTGKVGAEDADGIDMAYRVLADHARTITVALADGGRPDNTGRGYVLRRILRRAVRYAHEKLNASRGFFATLVDVVVQSLGDAFPELKKDPDMVKDIINEEEVQFLKTLSRGRRILDRKIQSLGDSKTIPGDTAWLLYDTYGFPVDLTGLIAEEKGLVVDMDGFEEERKLAQLKSQGKGAGGEDLIMLDIYAIEELRARGLEVTDDSPKYNYHLDSSGSYVFENTVATVMALRREKMFVEEVSTGQECGVVLDKTCFYAEQGGQIYDEGYLVKVDDSSEDKTEFTVKNAQVRGGYVLHIGTIYGDLKVGDQVWLFIDEPRRRPIMSNHTATHILNFALRSVLGEADQKGSLVAPDRLRFDFTAKGAMSTQQIKKAEEIANEMIEAAKAVYTQDCPLAAAKAIQGLRAVFDETYPDPVRVVSIGVPVSELLDDPSGPAGSLTSVEFCGGTHLRNSSHAGAFVIVTEEAIAKGIRRIVAVTGAEAQKALRKAESLKKCLSVMEAKVKAQTAPNKDVQREIADLGEALATAVIPQWQKDELRETLKSLKKVMDDLDRASKADVQKRVLEKTKQFIDSNPNQPLVILEMESGASAKALNEALKLFKMHSPQTSAMLFTVDNEAGKITCLCQVPQNAANRGLKASEWVQQVSGLMDGKGGGKDVSAQATGKNVGCLQEALQLATSFAQLRLGDVKN

Target ORF information:

RefSeq Version	NM_001605.3
Organism	Homo sapiens(human)
Definition	Homo sapiens alanyl-tRNA synthetase 1 (AARS1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001605.3

ORF Insert Sequence:

ATGGACTCTA CTCTAACAGC AAGTGAAATC CGGCAGCGAT TTATAGATTT CTTCAAGAGG 
AACGAGCATA CGTATGTTCA CTCGTCTGCC ACCATCCCAT TGGATGACCC CACTTTGCTC 
TTTGCCAATG CAGGCATGAA CCAGTTTAAA CCCATTTTCC TGAACACAAT TGACCCATCT 
CACCCCATGG CAAAGCTGAG CAGAGCTGCC AATACCCAGA AGTGCATCCG GGCTGGGGGC 
AAACATAATG ACCTGGACGA TGTGGGCAAG GATGTCTATC ATCACACCTT CTTCGAGATG 
CTGGGCTCTT GGTCTTTTGG AGATTACTTT AAGGAATTGG CATGTAAGAT GGCTCTGGAA 
CTCCTCACCC AAGAGTTTGG CATTCCCATT GAAAGACTTT ATGTTACTTA CTTTGGCGGG 
GATGAAGCAG CTGGCTTAGA AGCAGATCTG GAATGCAAAC AGATCTGGCA AAATTTGGGG 
CTGGATGACA CCAAAATCCT CCCAGGCAAC ATGAAGGATA ACTTCTGGGA GATGGGTGAC 
ACGGGCCCCT GTGGTCCTTG CAGTGAGATC CACTACGACC GGATTGGTGG TCGGGACGCC 
GCACATCTTG TCAACCAGGA CGACCCTAAT GTGCTGGAGA TCTGGAACCT TGTGTTCATC 
CAGTATAACA GGGAAGCTGA TGGCATTCTG AAACCTCTTC CCAAGAAAAG CATTGACACA 
GGGATGGGCC TGGAACGACT GGTATCTGTG CTGCAGAATA AGATGTCCAA CTATGACACT 
GACCTTTTTG TCCCTTACTT TGAAGCCATT CAGAAGGGCA CAGGTGCCCG ACCATACACT 
GGGAAAGTTG GTGCTGAGGA TGCCGATGGG ATTGACATGG CCTACCGGGT GCTGGCTGAC 
CACGCTCGGA CCATCACTGT GGCACTGGCT GATGGTGGCC GGCCTGACAA CACAGGGCGT 
GGATATGTGT TGAGACGGAT TCTCCGCCGA GCTGTCCGAT ACGCCCATGA AAAGCTCAAT 
GCCAGCAGGG GCTTCTTTGC TACGTTAGTG GATGTTGTCG TCCAGTCCCT GGGAGATGCA 
TTTCCTGAGC TGAAGAAGGA CCCAGACATG GTGAAGGACA TCATTAATGA AGAAGAGGTG 
CAGTTTCTCA AGACTCTCAG CAGAGGGCGT CGCATCCTGG ACAGGAAAAT TCAGAGCCTG 
GGAGACAGCA AGACCATTCC CGGAGACACT GCTTGGCTCC TCTATGACAC CTATGGGTTT 
CCAGTGGATC TGACTGGACT GATTGCTGAA GAGAAGGGCC TGGTGGTAGA CATGGATGGC 
TTTGAAGAGG AGAGGAAACT GGCCCAGCTG AAATCACAGG GCAAGGGAGC TGGTGGGGAA 
GACCTCATTA TGCTGGACAT TTACGCTATC GAAGAGCTCC GGGCACGGGG TCTGGAGGTC 
ACAGATGATT CCCCAAAGTA CAATTACCAT TTGGACTCCA GTGGTAGCTA TGTATTTGAG 
AACACAGTGG CTACGGTGAT GGCTCTGCGC AGGGAGAAGA TGTTCGTGGA AGAGGTGTCC 
ACAGGCCAGG AGTGTGGAGT GGTGCTGGAC AAGACCTGTT TCTATGCTGA GCAAGGAGGC 
CAGATCTATG ACGAAGGCTA CCTGGTGAAG GTGGATGACA GCAGTGAAGA TAAAACAGAG 
TTTACAGTGA AGAATGCTCA GGTCCGAGGA GGGTATGTGC TACACATTGG AACCATCTAC 
GGTGACCTGA AAGTGGGGGA TCAGGTCTGG CTGTTTATTG ATGAGCCCCG ACGAAGACCC 
ATCATGAGCA ACCACACAGC TACGCACATT CTGAACTTCG CCCTGCGCTC AGTGCTTGGG 
GAAGCTGACC AGAAAGGCTC ATTGGTTGCT CCTGACCGCC TCAGATTTGA CTTTACTGCC 
AAGGGAGCCA TGTCCACCCA ACAGATCAAG AAGGCTGAAG AGATTGCTAA TGAGATGATT 
GAGGCAGCCA AGGCCGTCTA TACCCAGGAT TGCCCCCTGG CAGCAGCGAA AGCCATCCAG 
GGCCTACGGG CTGTGTTTGA TGAGACCTAT CCTGACCCTG TGCGAGTCGT CTCCATTGGG 
GTCCCGGTGT CCGAGTTGCT GGATGACCCC TCTGGGCCTG CTGGCTCCCT GACTTCTGTT 
GAGTTCTGTG GGGGAACGCA CCTGCGGAAC TCGAGTCATG CAGGAGCTTT TGTGATCGTG 
ACGGAAGAAG CCATTGCCAA GGGTATCCGG AGGATTGTGG CTGTCACAGG TGCCGAGGCC 
CAGAAGGCCC TCAGGAAAGC AGAGAGCTTG AAGAAATGTC TCTCTGTCAT GGAAGCCAAA 
GTGAAGGCTC AGACTGCTCC AAACAAGGAT GTGCAGAGGG AGATCGCTGA CCTTGGAGAG 
GCCCTGGCCA CTGCAGTCAT CCCCCAGTGG CAGAAGGATG AATTGCGGGA GACTCTCAAA 
TCCCTAAAGA AGGTCATGGA TGACTTGGAC CGAGCCAGCA AAGCCGATGT CCAGAAACGA 
GTGTTAGAGA AGACGAAGCA GTTCATCGAC AGCAACCCCA ACCAGCCTCT TGTCATCCTG 
GAGATGGAGA GCGGCGCCTC AGCCAAGGCC CTGAATGAAG CCTTGAAGCT CTTCAAGATG 
CACTCCCCTC AGACTTCTGC CATGCTCTTC ACGGTGGACA ATGAGGCTGG CAAGATCACG 
TGCCTGTGTC AAGTTCCCCA GAATGCAGCC AATCGGGGCT TAAAAGCCAG CGAGTGGGTG 
CAGCAGGTGT CAGGCTTGAT GGACGGTAAA GGTGGTGGCA AGGATGTGTC TGCACAGGCC 
ACAGGCAAGA ACGTTGGCTG CCTGCAGGAG GCGCTGCAGC TGGCCACTTC CTTCGCCCAG 
CTGCGCCTCG GGGATGTAAA GAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Deficient activity of alanyl-tRNA synthetase underlies an autosomal recessive syndrome of progressive microcephaly, hypomyelination, and epileptic encephalopathy.
Human mutation38(10)1348-1354(2017 Oct)
Nakayama T,Wu J,Galvin-Parton P,Weiss J,Andriola MR,Hill RS,Vaughan DJ,El-Quessny M,Barry BJ,Partlow JN,Barkovich AJ,Ling J,Mochida GH

Two crystal structures reveal design for repurposing the C-Ala domain of human AlaRS.
Proceedings of the National Academy of Sciences of the United States of America113(50)14300-14305(2016 Dec)
Sun L,Song Y,Blocquel D,Yang XL,Schimmel P

Evolutionary Gain of Alanine Mischarging to Noncognate tRNAs with a G4:U69 Base Pair.
Journal of the American Chemical Society138(39)12948-12955(2016 Oct)
Sun L,Gomes AC,He W,Zhou H,Wang X,Pan DW,Schimmel P,Pan T,Yang XL

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AARS cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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