CYP27A1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	CYP27A1
Entrez Gene ID	1593
Full Name	cytochrome P450 family 27 subfamily A member 1
Synonyms	CP27,CTX,CYP27
General protein information	Preferred Names cytochrome P450 family 27 subfamily A member 1 Names sterol 26-hydroxylase, mitochondrial 5-beta-cholestane-3-alpha, 7-alpha, 12-alpha-triol 26-hydroxylase 5-beta-cholestane-3-alpha, 7-alpha, 12-alpha-triol 27-hydroxylase cholestanetriol 26-monooxygenase cytochrome P-450C27/25 cytochrome P450 27 cytochrome P450, family 27, subfamily A, polypeptide 1 cytochrome P450, subfamily XXVIIA (steroid 27-hydroxylase, cerebrotendinous xanthomatosis), polypeptide 1 sterol 27-hydroxylase vitamin D(3) 25-hydroxylase
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 2 Map Location: 2q35
Summary	This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This mitochondrial protein oxidizes cholesterol intermediates as part of the bile synthesis pathway. Since the conversion of cholesterol to bile acids is the major route for removing cholesterol from the body, this protein is important for overall cholesterol homeostasis. Mutations in this gene cause cerebrotendinous xanthomatosis, a rare autosomal recessive lipid storage disease. [provided by RefSeq, Jul 2008].
Disorder MIM:	606530
Disorder Html:	Cerebrotendinous xanthomatosis, 213700 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_000784.3	NP_000775.1	sterol 26-hydroxylase, mitochondrial precursor
XM_017003488.1	XP_016858977.1	sterol 26-hydroxylase, mitochondrial isoform X1
NM_000784.4	NP_000775.1	sterol 26-hydroxylase, mitochondrial precursor
XM_017003488.2	XP_016858977.1	sterol 26-hydroxylase, mitochondrial isoform X1

Pathways from BioSystems

Homologies

Bos taurus (cattle)	CYP27A1	NP_001076882.1
Danio rerio (zebrafish)	cyp27a7	XP_002663445.1
Xenopus tropicalis (tropical clawed frog)	cyp27a1	XP_002933986.2
Xenopus tropicalis (tropical clawed frog)	cyp27b1	XP_002934002.2
Homo sapiens (human)	CYP27A1	NP_000775.1
	CYP27A1	XP_001095424.1
Canis lupus familiaris (dog)	CYP27A1	XP_853094.3
Danio rerio (zebrafish)	cyp27a1.4	XP_001923080.3
Xenopus tropicalis (tropical clawed frog)	LOC100496109	XP_002933985.2
Xenopus tropicalis (tropical clawed frog)	cyp27a1	XP_002934001.2
Pan troglodytes (chimpanzee)	LOC459951	XP_001159436.1
Rattus norvegicus (Norway rat)	Cyp27a1	NP_849178.2
Danio rerio (zebrafish)	zgc:136333	NP_001038769.1
Danio rerio (zebrafish)	cyp27a1.2	XP_001334004.3
Drosophila melanogaster (fruit fly)	Cyp49a1	NP_001246256.1
Mus musculus (house mouse)	Cyp27a1	NP_077226.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005774	XP_315789.4
Gallus gallus (chicken)	CYP27A1	XP_422056.3

Gene Ontology
Bibliography

Related articles in PubMed

Clinical report: A patient with a late diagnosis of cerebrotendinous xanthomatosis and a response to treatment.
Alhariri A, Hamilton K, Oza V, Cordoro K, Sobreira NL, Malloy M, Slavotinek A
American journal of medical genetics. Part A173(8)2275-2279(2017 Aug)

Common genetic etiology between "multiple sclerosis-like" single-gene disorders and familial multiple sclerosis.
Traboulsee AL, Sadovnick AD, Encarnacion M, Bernales CQ, Yee IM, Criscuoli MG, Vilari?o-G?ell C
Human genetics136(6)705-714(2017 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Cerebrotendinous xanthomatosis .. is caused by mutations in the CYP27A1 gene that result in decreased production of chenodeoxycholic acid (CDCA) and elevated levels of cholestanol and bile alcohols. Sequencing of CYP27A1 showed a paternally inherited splice mutation, c.446 + 1G>T, and a maternally inherited nonsense mutation, c.808C>T, predicting p.(Arg270*).
Title: Clinical report: A patient with a late diagnosis of cerebrotendinous xanthomatosis and a response to treatment.

This study suggests a shared genetic etiology between MS and the characterized single-gene disorders, and highlights cholesterol metabolism and the synthesis of oxysterols as important biological mechanisms for familial MS
Title: Common genetic etiology between "multiple sclerosis-like" single-gene disorders and familial multiple sclerosis.

Increased maternal and fetal cholesterol efflux capacity and placental CYP27A1 expression in preeclampsia
Title: Increased maternal and fetal cholesterol efflux capacity and placental CYP27A1 expression in preeclampsia.

Molecular study identified a novel homozygous frameshift mutation in CYP27A1 gene in cerebrotendinous xanthomatosis without cataract
Title: A novel frameshift mutation in the sterol 27-hydroxylase gene in an Egyptian family with cerebrotendinous xanthomatosis without cataract.

The 32 kb deleted region harbors the CYP2A7 gene and was enriched with enhancer, repressor and insulator sites. The wildtype allele was lost in the polyps of the proband
Title: Chromosome 19q13 disruption alters expressions of CYP2A7, MIA and MIA-RAB4B lncRNA and contributes to FAP-like phenotype in APC mutation-negative familial colorectal cancer patients.

The following CYP27A1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CYP27A1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu26380	NM_000784.4 Latest version!	Homo sapiens cytochrome P450 family 27 subfamily A member 1 (CYP27A1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$219.50-$307.30 $439.00
OHu89087	XM_017003488.2 Latest version!	Homo sapiens cytochrome P450 family 27 subfamily A member 1 (CYP27A1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
View Old Accession Versions >>
OHu26380	NM_000784.3	Homo sapiens cytochrome P450 family 27 subfamily A member 1 (CYP27A1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$219.50-$307.30 $439.00
OHu89087	XM_017003488.1	Homo sapiens cytochrome P450 family 27 subfamily A member 1 (CYP27A1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CYP27A1 ( NM_000784.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_000775.1 Homo sapiens cytochrome P450 family 27 subfamily A member 1 (CYP27A1), mRNA.

Price & Availability↑

CloneID	OHu26380
Clone ID Related Accession (Same CDS sequence)	NM_000784.3 , NM_000784.4
Accession Version	NM_000784.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1596bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-05
Organism	Homo sapiens(human)
Product	sterol 26-hydroxylase, mitochondrial precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC009974.10, X59812.1 and BC051851.1. This sequence is a reference standard in the RefSeqGene project. On May 2, 2008 this sequence version replaced NM_000784.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.147036.1, SRR1803617.31095.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCTGCGC TGGGCTGCGC GAGGCTGAGG TGGGCGCTGC GAGGGGCCGG CCGTGGCCTC 
TGCCCCCACG GGGCCAGAGC CAAGGCCGCG ATCCCTGCCG CCCTCCCCTC GGACAAGGCC 
ACCGGAGCTC CCGGAGCCGG GCCTGGTGTC CGGCGGCGGC AACGGAGCTT AGAGGAGATT 
CCACGTCTAG GACAGCTGCG CTTCTTCTTT CAGCTGTTCG TTCAAGGCTA TGCCCTGCAA 
CTGCACCAGT TACAGGTGCT TTACAAGGCC AAGTACGGTC CAATGTGGAT GTCCTACTTA 
GGGCCTCAGA TGCACGTGAA CCTGGCCAGT GCCCCGCTCT TGGAGCAAGT GATGCGGCAA 
GAGGGCAAGT ACCCAGTACG GAACGACATG GAGCTATGGA AGGAGCACCG GGACCAGCAC 
GACCTGACCT ATGGGCCGTT CACCACGGAA GGACACCACT GGTACCAGCT GCGCCAGGCT 
CTGAACCAGC GGTTGCTGAA GCCAGCGGAA GCAGCGCTCT ATACGGATGC TTTCAATGAG 
GTGATTGATG ACTTTATGAC TCGACTGGAC CAGCTGCGGG CAGAGAGTGC TTCGGGGAAC 
CAGGTGTCGG ACATGGCTCA ACTCTTCTAC TACTTTGCCT TGGAAGCTAT TTGCTACATC 
CTGTTCGAGA AACGCATTGG CTGCCTGCAG CGATCCATCC CCGAGGACAC CGTGACCTTC 
GTCAGATCCA TCGGGTTAAT GTTCCAGAAC TCACTCTATG CCACCTTCCT CCCCAAGTGG 
ACTCGCCCCG TGCTGCCTTT CTGGAAGCGA TACCTGGATG GTTGGAATGC CATCTTTTCC 
TTTGGGAAGA AGCTGATTGA TGAGAAGCTC GAAGATATGG AGGCCCAACT GCAGGCAGCA 
GGGCCAGATG GCATCCAGGT GTCTGGCTAC CTGCACTTCT TACTGGCCAG TGGACAGCTC 
AGTCCTCGGG AGGCCATGGG CAGCCTGCCT GAGCTGCTCA TGGCTGGAGT GGACACGACA 
TCCAACACGC TGACATGGGC CCTGTACCAC CTCTCAAAGG ACCCTGAGAT CCAGGAGGCC 
TTGCACGAGG AAGTGGTGGG TGTGGTGCCA GCCGGGCAAG TGCCCCAGCA CAAGGACTTT 
GCCCACATGC CGTTGCTCAA AGCTGTGCTT AAGGAGACTC TGCGTCTCTA CCCTGTGGTC 
CCCACAAACT CCCGGATCAT AGAAAAGGAA ATTGAAGTTG ATGGCTTCCT CTTCCCCAAG 
AACACCCAGT TTGTGTTCTG CCACTATGTG GTGTCCCGGG ACCCCACTGC CTTCTCTGAG 
CCTGAAAGCT TCCAGCCCCA CCGCTGGCTG AGAAACAGCC AGCCTGCTAC CCCCAGGATC 
CAGCACCCAT TTGGCTCTGT GCCCTTTGGC TATGGGGTCC GGGCCTGCCT GGGCCGCAGG 
ATTGCAGAGC TGGAGATGCA GCTACTCCTC GCAAGGCTGA TCCAGAAGTA CAAGGTGGTC 
CTGGCCCCGG AGACGGGGGA GTTGAAGAGT GTGGCCCGCA TTGTCCTGGT TCCCAATAAG 
AAAGTGGGCC TGCAGTTCCT GCAGAGACAG TGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000775.1
CDS	435..2030
Translation	MAALGCARLRWALRGAGRGLCPHGARAKAAIPAALPSDKATGAPGAGPGVRRRQRSLEEIPRLGQLRFFFQLFVQGYALQLHQLQVLYKAKYGPMWMSYLGPQMHVNLASAPLLEQVMRQEGKYPVRNDMELWKEHRDQHDLTYGPFTTEGHHWYQLRQALNQRLLKPAEAALYTDAFNEVIDDFMTRLDQLRAESASGNQVSDMAQLFYYFALEAICYILFEKRIGCLQRSIPEDTVTFVRSIGLMFQNSLYATFLPKWTRPVLPFWKRYLDGWNAIFSFGKKLIDEKLEDMEAQLQAAGPDGIQVSGYLHFLLASGQLSPREAMGSLPELLMAGVDTTSNTLTWALYHLSKDPEIQEALHEEVVGVVPAGQVPQHKDFAHMPLLKAVLKETLRLYPVVPTNSRIIEKEIEVDGFLFPKNTQFVFCHYVVSRDPTAFSEPESFQPHRWLRNSQPATPRIQHPFGSVPFGYGVRACLGRRIAELEMQLLLARLIQKYKVVLAPETGELKSVARIVLVPNKKVGLQFLQRQC

Target ORF information:

RefSeq Version	NM_000784.3
Organism	Homo sapiens(human)
Definition	Homo sapiens cytochrome P450 family 27 subfamily A member 1 (CYP27A1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000784.3

ORF Insert Sequence:

ATGGCTGCGC TGGGCTGCGC GAGGCTGAGG TGGGCGCTGC GAGGGGCCGG CCGTGGCCTC 
TGCCCCCACG GGGCCAGAGC CAAGGCCGCG ATCCCTGCCG CCCTCCCCTC GGACAAGGCC 
ACCGGAGCTC CCGGAGCCGG GCCTGGTGTC CGGCGGCGGC AACGGAGCTT AGAGGAGATT 
CCACGTCTAG GACAGCTGCG CTTCTTCTTT CAGCTGTTCG TTCAAGGCTA TGCCCTGCAA 
CTGCACCAGT TACAGGTGCT TTACAAGGCC AAGTACGGTC CAATGTGGAT GTCCTACTTA 
GGGCCTCAGA TGCACGTGAA CCTGGCCAGT GCCCCGCTCT TGGAGCAAGT GATGCGGCAA 
GAGGGCAAGT ACCCAGTACG GAACGACATG GAGCTATGGA AGGAGCACCG GGACCAGCAC 
GACCTGACCT ATGGGCCGTT CACCACGGAA GGACACCACT GGTACCAGCT GCGCCAGGCT 
CTGAACCAGC GGTTGCTGAA GCCAGCGGAA GCAGCGCTCT ATACGGATGC TTTCAATGAG 
GTGATTGATG ACTTTATGAC TCGACTGGAC CAGCTGCGGG CAGAGAGTGC TTCGGGGAAC 
CAGGTGTCGG ACATGGCTCA ACTCTTCTAC TACTTTGCCT TGGAAGCTAT TTGCTACATC 
CTGTTCGAGA AACGCATTGG CTGCCTGCAG CGATCCATCC CCGAGGACAC CGTGACCTTC 
GTCAGATCCA TCGGGTTAAT GTTCCAGAAC TCACTCTATG CCACCTTCCT CCCCAAGTGG 
ACTCGCCCCG TGCTGCCTTT CTGGAAGCGA TACCTGGATG GTTGGAATGC CATCTTTTCC 
TTTGGGAAGA AGCTGATTGA TGAGAAGCTC GAAGATATGG AGGCCCAACT GCAGGCAGCA 
GGGCCAGATG GCATCCAGGT GTCTGGCTAC CTGCACTTCT TACTGGCCAG TGGACAGCTC 
AGTCCTCGGG AGGCCATGGG CAGCCTGCCT GAGCTGCTCA TGGCTGGAGT GGACACGACA 
TCCAACACGC TGACATGGGC CCTGTACCAC CTCTCAAAGG ACCCTGAGAT CCAGGAGGCC 
TTGCACGAGG AAGTGGTGGG TGTGGTGCCA GCCGGGCAAG TGCCCCAGCA CAAGGACTTT 
GCCCACATGC CGTTGCTCAA AGCTGTGCTT AAGGAGACTC TGCGTCTCTA CCCTGTGGTC 
CCCACAAACT CCCGGATCAT AGAAAAGGAA ATTGAAGTTG ATGGCTTCCT CTTCCCCAAG 
AACACCCAGT TTGTGTTCTG CCACTATGTG GTGTCCCGGG ACCCCACTGC CTTCTCTGAG 
CCTGAAAGCT TCCAGCCCCA CCGCTGGCTG AGAAACAGCC AGCCTGCTAC CCCCAGGATC 
CAGCACCCAT TTGGCTCTGT GCCCTTTGGC TATGGGGTCC GGGCCTGCCT GGGCCGCAGG 
ATTGCAGAGC TGGAGATGCA GCTACTCCTC GCAAGGCTGA TCCAGAAGTA CAAGGTGGTC 
CTGGCCCCGG AGACGGGGGA GTTGAAGAGT GTGGCCCGCA TTGTCCTGGT TCCCAATAAG 
AAAGTGGGCC TGCAGTTCCT GCAGAGACAG TGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CYP27A1 ( NM_000784.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_000775.1 Homo sapiens cytochrome P450 family 27 subfamily A member 1 (CYP27A1), mRNA.

Price & Availability↑

CloneID	OHu26380
Clone ID Related Accession (Same CDS sequence)	NM_000784.3 , NM_000784.4
Accession Version	NM_000784.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1596bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-10
Organism	Homo sapiens(human)
Product	sterol 26-hydroxylase, mitochondrial precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from X59812.1 and BC051851.1. This sequence is a reference standard in the RefSeqGene project. On Nov 23, 2018 this sequence version replaced NM_000784.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X59812.1, SRR3476690.1150863.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta MANE Ensembl match :: ENST00000258415.9/ ENSP00000258415.4 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCTGCGC TGGGCTGCGC GAGGCTGAGG TGGGCGCTGC GAGGGGCCGG CCGTGGCCTC 
TGCCCCCACG GGGCCAGAGC CAAGGCCGCG ATCCCTGCCG CCCTCCCCTC GGACAAGGCC 
ACCGGAGCTC CCGGAGCCGG GCCTGGTGTC CGGCGGCGGC AACGGAGCTT AGAGGAGATT 
CCACGTCTAG GACAGCTGCG CTTCTTCTTT CAGCTGTTCG TTCAAGGCTA TGCCCTGCAA 
CTGCACCAGT TACAGGTGCT TTACAAGGCC AAGTACGGTC CAATGTGGAT GTCCTACTTA 
GGGCCTCAGA TGCACGTGAA CCTGGCCAGT GCCCCGCTCT TGGAGCAAGT GATGCGGCAA 
GAGGGCAAGT ACCCAGTACG GAACGACATG GAGCTATGGA AGGAGCACCG GGACCAGCAC 
GACCTGACCT ATGGGCCGTT CACCACGGAA GGACACCACT GGTACCAGCT GCGCCAGGCT 
CTGAACCAGC GGTTGCTGAA GCCAGCGGAA GCAGCGCTCT ATACGGATGC TTTCAATGAG 
GTGATTGATG ACTTTATGAC TCGACTGGAC CAGCTGCGGG CAGAGAGTGC TTCGGGGAAC 
CAGGTGTCGG ACATGGCTCA ACTCTTCTAC TACTTTGCCT TGGAAGCTAT TTGCTACATC 
CTGTTCGAGA AACGCATTGG CTGCCTGCAG CGATCCATCC CCGAGGACAC CGTGACCTTC 
GTCAGATCCA TCGGGTTAAT GTTCCAGAAC TCACTCTATG CCACCTTCCT CCCCAAGTGG 
ACTCGCCCCG TGCTGCCTTT CTGGAAGCGA TACCTGGATG GTTGGAATGC CATCTTTTCC 
TTTGGGAAGA AGCTGATTGA TGAGAAGCTC GAAGATATGG AGGCCCAACT GCAGGCAGCA 
GGGCCAGATG GCATCCAGGT GTCTGGCTAC CTGCACTTCT TACTGGCCAG TGGACAGCTC 
AGTCCTCGGG AGGCCATGGG CAGCCTGCCT GAGCTGCTCA TGGCTGGAGT GGACACGACA 
TCCAACACGC TGACATGGGC CCTGTACCAC CTCTCAAAGG ACCCTGAGAT CCAGGAGGCC 
TTGCACGAGG AAGTGGTGGG TGTGGTGCCA GCCGGGCAAG TGCCCCAGCA CAAGGACTTT 
GCCCACATGC CGTTGCTCAA AGCTGTGCTT AAGGAGACTC TGCGTCTCTA CCCTGTGGTC 
CCCACAAACT CCCGGATCAT AGAAAAGGAA ATTGAAGTTG ATGGCTTCCT CTTCCCCAAG 
AACACCCAGT TTGTGTTCTG CCACTATGTG GTGTCCCGGG ACCCCACTGC CTTCTCTGAG 
CCTGAAAGCT TCCAGCCCCA CCGCTGGCTG AGAAACAGCC AGCCTGCTAC CCCCAGGATC 
CAGCACCCAT TTGGCTCTGT GCCCTTTGGC TATGGGGTCC GGGCCTGCCT GGGCCGCAGG 
ATTGCAGAGC TGGAGATGCA GCTACTCCTC GCAAGGCTGA TCCAGAAGTA CAAGGTGGTC 
CTGGCCCCGG AGACGGGGGA GTTGAAGAGT GTGGCCCGCA TTGTCCTGGT TCCCAATAAG 
AAAGTGGGCC TGCAGTTCCT GCAGAGACAG TGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000775.1
CDS	37..1632
Translation	MAALGCARLRWALRGAGRGLCPHGARAKAAIPAALPSDKATGAPGAGPGVRRRQRSLEEIPRLGQLRFFFQLFVQGYALQLHQLQVLYKAKYGPMWMSYLGPQMHVNLASAPLLEQVMRQEGKYPVRNDMELWKEHRDQHDLTYGPFTTEGHHWYQLRQALNQRLLKPAEAALYTDAFNEVIDDFMTRLDQLRAESASGNQVSDMAQLFYYFALEAICYILFEKRIGCLQRSIPEDTVTFVRSIGLMFQNSLYATFLPKWTRPVLPFWKRYLDGWNAIFSFGKKLIDEKLEDMEAQLQAAGPDGIQVSGYLHFLLASGQLSPREAMGSLPELLMAGVDTTSNTLTWALYHLSKDPEIQEALHEEVVGVVPAGQVPQHKDFAHMPLLKAVLKETLRLYPVVPTNSRIIEKEIEVDGFLFPKNTQFVFCHYVVSRDPTAFSEPESFQPHRWLRNSQPATPRIQHPFGSVPFGYGVRACLGRRIAELEMQLLLARLIQKYKVVLAPETGELKSVARIVLVPNKKVGLQFLQRQC

Target ORF information:

RefSeq Version	NM_000784.4
Organism	Homo sapiens(human)
Definition	Homo sapiens cytochrome P450 family 27 subfamily A member 1 (CYP27A1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000784.4

ORF Insert Sequence:

ATGGCTGCGC TGGGCTGCGC GAGGCTGAGG TGGGCGCTGC GAGGGGCCGG CCGTGGCCTC 
TGCCCCCACG GGGCCAGAGC CAAGGCCGCG ATCCCTGCCG CCCTCCCCTC GGACAAGGCC 
ACCGGAGCTC CCGGAGCCGG GCCTGGTGTC CGGCGGCGGC AACGGAGCTT AGAGGAGATT 
CCACGTCTAG GACAGCTGCG CTTCTTCTTT CAGCTGTTCG TTCAAGGCTA TGCCCTGCAA 
CTGCACCAGT TACAGGTGCT TTACAAGGCC AAGTACGGTC CAATGTGGAT GTCCTACTTA 
GGGCCTCAGA TGCACGTGAA CCTGGCCAGT GCCCCGCTCT TGGAGCAAGT GATGCGGCAA 
GAGGGCAAGT ACCCAGTACG GAACGACATG GAGCTATGGA AGGAGCACCG GGACCAGCAC 
GACCTGACCT ATGGGCCGTT CACCACGGAA GGACACCACT GGTACCAGCT GCGCCAGGCT 
CTGAACCAGC GGTTGCTGAA GCCAGCGGAA GCAGCGCTCT ATACGGATGC TTTCAATGAG 
GTGATTGATG ACTTTATGAC TCGACTGGAC CAGCTGCGGG CAGAGAGTGC TTCGGGGAAC 
CAGGTGTCGG ACATGGCTCA ACTCTTCTAC TACTTTGCCT TGGAAGCTAT TTGCTACATC 
CTGTTCGAGA AACGCATTGG CTGCCTGCAG CGATCCATCC CCGAGGACAC CGTGACCTTC 
GTCAGATCCA TCGGGTTAAT GTTCCAGAAC TCACTCTATG CCACCTTCCT CCCCAAGTGG 
ACTCGCCCCG TGCTGCCTTT CTGGAAGCGA TACCTGGATG GTTGGAATGC CATCTTTTCC 
TTTGGGAAGA AGCTGATTGA TGAGAAGCTC GAAGATATGG AGGCCCAACT GCAGGCAGCA 
GGGCCAGATG GCATCCAGGT GTCTGGCTAC CTGCACTTCT TACTGGCCAG TGGACAGCTC 
AGTCCTCGGG AGGCCATGGG CAGCCTGCCT GAGCTGCTCA TGGCTGGAGT GGACACGACA 
TCCAACACGC TGACATGGGC CCTGTACCAC CTCTCAAAGG ACCCTGAGAT CCAGGAGGCC 
TTGCACGAGG AAGTGGTGGG TGTGGTGCCA GCCGGGCAAG TGCCCCAGCA CAAGGACTTT 
GCCCACATGC CGTTGCTCAA AGCTGTGCTT AAGGAGACTC TGCGTCTCTA CCCTGTGGTC 
CCCACAAACT CCCGGATCAT AGAAAAGGAA ATTGAAGTTG ATGGCTTCCT CTTCCCCAAG 
AACACCCAGT TTGTGTTCTG CCACTATGTG GTGTCCCGGG ACCCCACTGC CTTCTCTGAG 
CCTGAAAGCT TCCAGCCCCA CCGCTGGCTG AGAAACAGCC AGCCTGCTAC CCCCAGGATC 
CAGCACCCAT TTGGCTCTGT GCCCTTTGGC TATGGGGTCC GGGCCTGCCT GGGCCGCAGG 
ATTGCAGAGC TGGAGATGCA GCTACTCCTC GCAAGGCTGA TCCAGAAGTA CAAGGTGGTC 
CTGGCCCCGG AGACGGGGGA GTTGAAGAGT GTGGCCCGCA TTGTCCTGGT TCCCAATAAG 
AAAGTGGGCC TGCAGTTCCT GCAGAGACAG TGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CYP27A1 ( XM_017003488.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016858977.1 Homo sapiens cytochrome P450 family 27 subfamily A member 1 (CYP27A1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu89087
Clone ID Related Accession (Same CDS sequence)	XM_017003488.1 , XM_017003488.2
Accession Version	XM_017003488.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1176bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	sterol 26-hydroxylase, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCTGCA ACTGCACCAG TTACAGGGAA GGACACCACT GGTACCAGCT GCGCCAGGCT 
CTGAACCAGC GGTTGCTGAA GCCAGCGGAA GCAGCGCTCT ATACGGATGC TTTCAATGAG 
GTGATTGATG ACTTTATGAC TCGACTGGAC CAGCTGCGGG CAGAGAGTGC TTCGGGGAAC 
CAGGTGTCGG ACATGGCTCA ACTCTTCTAC TACTTTGCCT TGGAAGCTAT TTGCTACATC 
CTGTTCGAGA AACGCATTGG CTGCCTGCAG CGATCCATCC CCGAGGACAC CGTGACCTTC 
GTCAGATCCA TCGGGTTAAT GTTCCAGAAC TCACTCTATG CCACCTTCCT CCCCAAGTGG 
ACTCGCCCCG TGCTGCCTTT CTGGAAGCGA TACCTGGATG GTTGGAATGC CATCTTTTCC 
TTTGGGAAGA AGCTGATTGA TGAGAAGCTC GAAGATATGG AGGCCCAACT GCAGGCAGCA 
GGGCCAGATG GCATCCAGGT GTCTGGCTAC CTGCACTTCT TACTGGCCAG TGGACAGCTC 
AGTCCTCGGG AGGCCATGGG CAGCCTGCCT GAGCTGCTCA TGGCTGGAGT GGACACGACA 
TCCAACACGC TGACATGGGC CCTGTACCAC CTCTCAAAGG ACCCTGAGAT CCAGGAGGCC 
TTGCACGAGG AAGTGGTGGG TGTGGTGCCA GCCGGGCAAG TGCCCCAGCA CAAGGACTTT 
GCCCACATGC CGTTGCTCAA AGCTGTGCTT AAGGAGACTC TGCGTCTCTA CCCTGTGGTC 
CCCACAAACT CCCGGATCAT AGAAAAGGAA ATTGAAGTTG ATGGCTTCCT CTTCCCCAAG 
AACACCCAGT TTGTGTTCTG CCACTATGTG GTGTCCCGGG ACCCCACTGC CTTCTCTGAG 
CCTGAAAGCT TCCAGCCCCA CCGCTGGCTG AGAAACAGCC AGCCTGCTAC CCCCAGGATC 
CAGCACCCAT TTGGCTCTGT GCCCTTTGGC TATGGGGTCC GGGCCTGCCT GGGCCGCAGG 
ATTGCAGAGC TGGAGATGCA GCTACTCCTC GCAAGGCTGA TCCAGAAGTA CAAGGTGGTC 
CTGGCCCCGG AGACGGGGGA GTTGAAGAGT GTGGCCCGCA TTGTCCTGGT TCCCAATAAG 
AAAGTGGGCC TGCAGTTCCT GCAGAGACAG TGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016858977.1
CDS	1407..2582
Translation	MPCNCTSYREGHHWYQLRQALNQRLLKPAEAALYTDAFNEVIDDFMTRLDQLRAESASGNQVSDMAQLFYYFALEAICYILFEKRIGCLQRSIPEDTVTFVRSIGLMFQNSLYATFLPKWTRPVLPFWKRYLDGWNAIFSFGKKLIDEKLEDMEAQLQAAGPDGIQVSGYLHFLLASGQLSPREAMGSLPELLMAGVDTTSNTLTWALYHLSKDPEIQEALHEEVVGVVPAGQVPQHKDFAHMPLLKAVLKETLRLYPVVPTNSRIIEKEIEVDGFLFPKNTQFVFCHYVVSRDPTAFSEPESFQPHRWLRNSQPATPRIQHPFGSVPFGYGVRACLGRRIAELEMQLLLARLIQKYKVVLAPETGELKSVARIVLVPNKKVGLQFLQRQC

Target ORF information:

RefSeq Version	XM_017003488.1
Organism	Homo sapiens(human)
Definition	Homo sapiens cytochrome P450 family 27 subfamily A member 1 (CYP27A1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017003488.1

ORF Insert Sequence:

ATGCCCTGCA ACTGCACCAG TTACAGGGAA GGACACCACT GGTACCAGCT GCGCCAGGCT 
CTGAACCAGC GGTTGCTGAA GCCAGCGGAA GCAGCGCTCT ATACGGATGC TTTCAATGAG 
GTGATTGATG ACTTTATGAC TCGACTGGAC CAGCTGCGGG CAGAGAGTGC TTCGGGGAAC 
CAGGTGTCGG ACATGGCTCA ACTCTTCTAC TACTTTGCCT TGGAAGCTAT TTGCTACATC 
CTGTTCGAGA AACGCATTGG CTGCCTGCAG CGATCCATCC CCGAGGACAC CGTGACCTTC 
GTCAGATCCA TCGGGTTAAT GTTCCAGAAC TCACTCTATG CCACCTTCCT CCCCAAGTGG 
ACTCGCCCCG TGCTGCCTTT CTGGAAGCGA TACCTGGATG GTTGGAATGC CATCTTTTCC 
TTTGGGAAGA AGCTGATTGA TGAGAAGCTC GAAGATATGG AGGCCCAACT GCAGGCAGCA 
GGGCCAGATG GCATCCAGGT GTCTGGCTAC CTGCACTTCT TACTGGCCAG TGGACAGCTC 
AGTCCTCGGG AGGCCATGGG CAGCCTGCCT GAGCTGCTCA TGGCTGGAGT GGACACGACA 
TCCAACACGC TGACATGGGC CCTGTACCAC CTCTCAAAGG ACCCTGAGAT CCAGGAGGCC 
TTGCACGAGG AAGTGGTGGG TGTGGTGCCA GCCGGGCAAG TGCCCCAGCA CAAGGACTTT 
GCCCACATGC CGTTGCTCAA AGCTGTGCTT AAGGAGACTC TGCGTCTCTA CCCTGTGGTC 
CCCACAAACT CCCGGATCAT AGAAAAGGAA ATTGAAGTTG ATGGCTTCCT CTTCCCCAAG 
AACACCCAGT TTGTGTTCTG CCACTATGTG GTGTCCCGGG ACCCCACTGC CTTCTCTGAG 
CCTGAAAGCT TCCAGCCCCA CCGCTGGCTG AGAAACAGCC AGCCTGCTAC CCCCAGGATC 
CAGCACCCAT TTGGCTCTGT GCCCTTTGGC TATGGGGTCC GGGCCTGCCT GGGCCGCAGG 
ATTGCAGAGC TGGAGATGCA GCTACTCCTC GCAAGGCTGA TCCAGAAGTA CAAGGTGGTC 
CTGGCCCCGG AGACGGGGGA GTTGAAGAGT GTGGCCCGCA TTGTCCTGGT TCCCAATAAG 
AAAGTGGGCC TGCAGTTCCT GCAGAGACAG TGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CYP27A1 ( XM_017003488.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016858977.1 Homo sapiens cytochrome P450 family 27 subfamily A member 1 (CYP27A1), transcript variant X1, mRNA.

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CloneID	OHu89087
Clone ID Related Accession (Same CDS sequence)	XM_017003488.1 , XM_017003488.2
Accession Version	XM_017003488.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1176bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	sterol 26-hydroxylase, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017003488.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCTGCA ACTGCACCAG TTACAGGGAA GGACACCACT GGTACCAGCT GCGCCAGGCT 
CTGAACCAGC GGTTGCTGAA GCCAGCGGAA GCAGCGCTCT ATACGGATGC TTTCAATGAG 
GTGATTGATG ACTTTATGAC TCGACTGGAC CAGCTGCGGG CAGAGAGTGC TTCGGGGAAC 
CAGGTGTCGG ACATGGCTCA ACTCTTCTAC TACTTTGCCT TGGAAGCTAT TTGCTACATC 
CTGTTCGAGA AACGCATTGG CTGCCTGCAG CGATCCATCC CCGAGGACAC CGTGACCTTC 
GTCAGATCCA TCGGGTTAAT GTTCCAGAAC TCACTCTATG CCACCTTCCT CCCCAAGTGG 
ACTCGCCCCG TGCTGCCTTT CTGGAAGCGA TACCTGGATG GTTGGAATGC CATCTTTTCC 
TTTGGGAAGA AGCTGATTGA TGAGAAGCTC GAAGATATGG AGGCCCAACT GCAGGCAGCA 
GGGCCAGATG GCATCCAGGT GTCTGGCTAC CTGCACTTCT TACTGGCCAG TGGACAGCTC 
AGTCCTCGGG AGGCCATGGG CAGCCTGCCT GAGCTGCTCA TGGCTGGAGT GGACACGACA 
TCCAACACGC TGACATGGGC CCTGTACCAC CTCTCAAAGG ACCCTGAGAT CCAGGAGGCC 
TTGCACGAGG AAGTGGTGGG TGTGGTGCCA GCCGGGCAAG TGCCCCAGCA CAAGGACTTT 
GCCCACATGC CGTTGCTCAA AGCTGTGCTT AAGGAGACTC TGCGTCTCTA CCCTGTGGTC 
CCCACAAACT CCCGGATCAT AGAAAAGGAA ATTGAAGTTG ATGGCTTCCT CTTCCCCAAG 
AACACCCAGT TTGTGTTCTG CCACTATGTG GTGTCCCGGG ACCCCACTGC CTTCTCTGAG 
CCTGAAAGCT TCCAGCCCCA CCGCTGGCTG AGAAACAGCC AGCCTGCTAC CCCCAGGATC 
CAGCACCCAT TTGGCTCTGT GCCCTTTGGC TATGGGGTCC GGGCCTGCCT GGGCCGCAGG 
ATTGCAGAGC TGGAGATGCA GCTACTCCTC GCAAGGCTGA TCCAGAAGTA CAAGGTGGTC 
CTGGCCCCGG AGACGGGGGA GTTGAAGAGT GTGGCCCGCA TTGTCCTGGT TCCCAATAAG 
AAAGTGGGCC TGCAGTTCCT GCAGAGACAG TGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016858977.1
CDS	680..1855
Translation	MPCNCTSYREGHHWYQLRQALNQRLLKPAEAALYTDAFNEVIDDFMTRLDQLRAESASGNQVSDMAQLFYYFALEAICYILFEKRIGCLQRSIPEDTVTFVRSIGLMFQNSLYATFLPKWTRPVLPFWKRYLDGWNAIFSFGKKLIDEKLEDMEAQLQAAGPDGIQVSGYLHFLLASGQLSPREAMGSLPELLMAGVDTTSNTLTWALYHLSKDPEIQEALHEEVVGVVPAGQVPQHKDFAHMPLLKAVLKETLRLYPVVPTNSRIIEKEIEVDGFLFPKNTQFVFCHYVVSRDPTAFSEPESFQPHRWLRNSQPATPRIQHPFGSVPFGYGVRACLGRRIAELEMQLLLARLIQKYKVVLAPETGELKSVARIVLVPNKKVGLQFLQRQC

Target ORF information:

RefSeq Version	XM_017003488.2
Organism	Homo sapiens(human)
Definition	Homo sapiens cytochrome P450 family 27 subfamily A member 1 (CYP27A1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017003488.2

ORF Insert Sequence:

ATGCCCTGCA ACTGCACCAG TTACAGGGAA GGACACCACT GGTACCAGCT GCGCCAGGCT 
CTGAACCAGC GGTTGCTGAA GCCAGCGGAA GCAGCGCTCT ATACGGATGC TTTCAATGAG 
GTGATTGATG ACTTTATGAC TCGACTGGAC CAGCTGCGGG CAGAGAGTGC TTCGGGGAAC 
CAGGTGTCGG ACATGGCTCA ACTCTTCTAC TACTTTGCCT TGGAAGCTAT TTGCTACATC 
CTGTTCGAGA AACGCATTGG CTGCCTGCAG CGATCCATCC CCGAGGACAC CGTGACCTTC 
GTCAGATCCA TCGGGTTAAT GTTCCAGAAC TCACTCTATG CCACCTTCCT CCCCAAGTGG 
ACTCGCCCCG TGCTGCCTTT CTGGAAGCGA TACCTGGATG GTTGGAATGC CATCTTTTCC 
TTTGGGAAGA AGCTGATTGA TGAGAAGCTC GAAGATATGG AGGCCCAACT GCAGGCAGCA 
GGGCCAGATG GCATCCAGGT GTCTGGCTAC CTGCACTTCT TACTGGCCAG TGGACAGCTC 
AGTCCTCGGG AGGCCATGGG CAGCCTGCCT GAGCTGCTCA TGGCTGGAGT GGACACGACA 
TCCAACACGC TGACATGGGC CCTGTACCAC CTCTCAAAGG ACCCTGAGAT CCAGGAGGCC 
TTGCACGAGG AAGTGGTGGG TGTGGTGCCA GCCGGGCAAG TGCCCCAGCA CAAGGACTTT 
GCCCACATGC CGTTGCTCAA AGCTGTGCTT AAGGAGACTC TGCGTCTCTA CCCTGTGGTC 
CCCACAAACT CCCGGATCAT AGAAAAGGAA ATTGAAGTTG ATGGCTTCCT CTTCCCCAAG 
AACACCCAGT TTGTGTTCTG CCACTATGTG GTGTCCCGGG ACCCCACTGC CTTCTCTGAG 
CCTGAAAGCT TCCAGCCCCA CCGCTGGCTG AGAAACAGCC AGCCTGCTAC CCCCAGGATC 
CAGCACCCAT TTGGCTCTGT GCCCTTTGGC TATGGGGTCC GGGCCTGCCT GGGCCGCAGG 
ATTGCAGAGC TGGAGATGCA GCTACTCCTC GCAAGGCTGA TCCAGAAGTA CAAGGTGGTC 
CTGGCCCCGG AGACGGGGGA GTTGAAGAGT GTGGCCCGCA TTGTCCTGGT TCCCAATAAG 
AAAGTGGGCC TGCAGTTCCT GCAGAGACAG TGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Clinical report: A patient with a late diagnosis of cerebrotendinous xanthomatosis and a response to treatment.
American journal of medical genetics. Part A173(8)2275-2279(2017 Aug)
Alhariri A,Hamilton K,Oza V,Cordoro K,Sobreira NL,Malloy M,Slavotinek A

Common genetic etiology between "multiple sclerosis-like" single-gene disorders and familial multiple sclerosis.
Human genetics136(6)705-714(2017 Jun)
Traboulsee AL,Sadovnick AD,Encarnacion M,Bernales CQ,Yee IM,Criscuoli MG,Vilari?o-G?ell C

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CYP27A1 cDNA ORF clone, Homo sapiens(human)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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