CYP21A2 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	CYP21A2
Entrez Gene ID	1589
Full Name	cytochrome P450 family 21 subfamily A member 2
Synonyms	CA21H,CAH1,CPS1,CYP21,CYP21B,P450c21B
General protein information	Preferred Names cytochrome P450 family 21 subfamily A member 2 Names steroid 21-hydroxylase 21-OHase cytochrome P450 XXI cytochrome P450, family 21, subfamily A, polypeptide 2 cytochrome P450, subfamily XXIA (steroid 21-hydroxylase, congenital adrenal hyperplasia), polypeptide 2 cytochrome P450-C21B steroid 21 hydroxylase steroid 21-monooxygenase
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 6 Map Location: 6p21.33
Summary	This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and hydroxylates steroids at the 21 position. Its activity is required for the synthesis of steroid hormones including cortisol and aldosterone. Mutations in this gene cause congenital adrenal hyperplasia. A related pseudogene is located near this gene; gene conversion events involving the functional gene and the pseudogene are thought to account for many cases of steroid 21-hydroxylase deficiency. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008].
Disorder MIM:	613815
Disorder Html:	Adrenal hyperplasia, congenital, due to 21-hydroxylase deficiency, 201910 (3); Hyperandrogenism, nonclassic type, due to 21-hydroxylase deficiency, 201910 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001368143.1	NP_001355072.1	steroid 21-hydroxylase isoform c
XM_024452556.1	XP_024308324.1	steroid 21-hydroxylase isoform X2
NM_001128590.3	NP_001122062.3	steroid 21-hydroxylase isoform b
XM_024452555.1	XP_024308323.1	steroid 21-hydroxylase isoform X1
NM_000500.7	NP_000491.4	steroid 21-hydroxylase isoform a
NM_000500.9	NP_000491.4	steroid 21-hydroxylase isoform a
XM_024452557.1	XP_024308325.1	steroid 21-hydroxylase isoform X3
NM_001368144.1	NP_001355073.1	steroid 21-hydroxylase isoform c

Pathways from BioSystems

Homologies

Homo sapiens (human)	CYP21A2	NP_000491.4
Xenopus tropicalis (tropical clawed frog)	LOC100489813	XP_002941314.2
Pan troglodytes (chimpanzee)	LOC745345	XP_003311237.1
Gallus gallus (chicken)	CYP21A2	NP_001092828.1
Mus musculus (house mouse)	Cyp21a1	NP_034125.2
Danio rerio (zebrafish)	LOC793249	XP_005157984.1
	CYP21A2	XP_001114240.1
Canis lupus familiaris (dog)	CYP21A2	NP_001003335.1
Rattus norvegicus (Norway rat)	Cyp21a1	NP_476442.2
Bos taurus (cattle)	CYP21	NP_001013614.1

Gene Ontology
Bibliography

Related articles in PubMed

Sensitive Monogenic Noninvasive Prenatal Diagnosis by Targeted Haplotyping.
Vermeulen C, Geeven G, de Wit E, Verstegen MJAM, Jansen RPM, van Kranenburg M, de Bruijn E, Pulit SL, Kruisselbrink E, Shahsavari Z, Omrani D, Zeinali F, Najmabadi H, Katsila T, Vrettou C, Patrinos GP, Traeger-Synodinos J, Splinter E, Beekman JM, Kheradmand Kia S, Te Meerman GJ, Ploos van Amstel HK, de Laat W
American journal of human genetics101(3)326-339(2017 Sep)

Functional analysis of human cytochrome P450 21A2 variants involved in congenital adrenal hyperplasia.
Wang C, Pallan PS, Zhang W, Lei L, Yoshimoto FK, Waterman MR, Egli M, Guengerich FP
The Journal of biological chemistry292(26)10767-10778(2017 06)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

A unique haplotype of RCCX copy number variation harboring a CYP21A2 identified in congenital adrenal hyperplasia patients.
Title: A unique haplotype of RCCX copy number variation: from the clinics of congenital adrenal hyperplasia to evolutionary genetics.

in-depth investigation of congenital adrenal hyperplasia-associated P450 21A2 variants reveals critical insight into the effects of disease-causing mutations on this important enzyme.
Title: Functional analysis of human cytochrome P450 21A2 variants involved in congenital adrenal hyperplasia.

Nine known mutations have been found in Chinese patients with 21-hydroxylase deficiency.
Title: Molecular diagnosis of Chinese patients with 21-hydroxylase deficiency and analysis of genotype-phenotype correlations.

16(beta)-OH-androstenedione generated by CYP21A2 serves as a substrate for CYP19A1
Title: The steroid metabolite 16(?)-OH-androstenedione generated by CYP21A2 serves as a substrate for CYP19A1.

There seems to be a specific spectrum of CYP21A2 gene mutations in Fujian area.
Title: [Analysis of CYP21A2 gene mutations among patients with classical steroid 21-hydroxylase deficiency].

The following CYP21A2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CYP21A2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu21462	NM_000500.9 Latest version!	Homo sapiens cytochrome P450 family 21 subfamily A member 2 (CYP21A2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu21027	NM_001128590.3 Latest version!	Homo sapiens cytochrome P450 family 21 subfamily A member 2 (CYP21A2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OHu57180	NM_001368143.1 Latest version!	Homo sapiens cytochrome P450 family 21 subfamily A member 2 (CYP21A2), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OHu57180	NM_001368144.1 Latest version!	Homo sapiens cytochrome P450 family 21 subfamily A member 2 (CYP21A2), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OHu57180	XM_024452557.1 Latest version!	Homo sapiens cytochrome P450 family 21 subfamily A member 2 (CYP21A2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OHu106316	XM_024452555.1 Latest version!	Homo sapiens cytochrome P450 family 21 subfamily A member 2 (CYP21A2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OHu106317	XM_024452556.1 Latest version!	Homo sapiens cytochrome P450 family 21 subfamily A member 2 (CYP21A2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
View Old Accession Versions >>
OHu21462	NM_000500.7	Homo sapiens cytochrome P450 family 21 subfamily A member 2 (CYP21A2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CYP21A2 ( NM_000500.7 ) cDNA ORF clone, Homo sapiens(human) -> NP_000491.4 Homo sapiens cytochrome P450 family 21 subfamily A member 2 (CYP21A2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu21462
Clone ID Related Accession (Same CDS sequence)	NM_000500.7 , NM_000500.9
Accession Version	NM_000500.7	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1488bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-17
Organism	Homo sapiens(human)
Product	steroid 21-hydroxylase isoform a
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AV708092.1, DA008890.1, BC125181.1, CD013985.1, M17252.1 and AL645922.12. This sequence is a reference standard in the RefSeqGene project. On Feb 17, 2011 this sequence version replaced NM_000500.6. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC125182.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145544, SAMEA2146411 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

ATGCTGCTCC TGGGCCTGCT GCTGCTGCTG CCCCTGCTGG CTGGCGCCCG CCTGCTGTGG 
AACTGGTGGA AGCTCCGGAG CCTCCACCTC CCGCCTCTTG CCCCGGGCTT CTTGCACCTG 
CTGCAGCCCG ACCTCCCCAT CTATCTGCTT GGCCTGACTC AGAAATTCGG GCCCATCTAC 
AGGCTCCACC TTGGGCTGCA AGATGTGGTG GTGCTGAACT CCAAGAGGAC CATTGAGGAA 
GCCATGGTCA AAAAGTGGGC AGACTTTGCT GGCAGACCTG AGCCACTTAC CTACAAGCTG 
GTGTCTAGGA ACTACCCGGA CCTGTCCTTG GGAGACTACT CCCTGCTCTG GAAAGCCCAC 
AAGAAGCTCA CCCGCTCAGC CCTGCTGCTG GGCATCCGTG ACTCCATGGA GCCAGTGGTG 
GAGCAGCTGA CCCAGGAGTT CTGTGAGCGC ATGAGAGCCC AGCCCGGCAC CCCTGTGGCC 
ATTGAGGAGG AATTCTCTCT CCTCACCTGC AGCATCATCT GTTACCTCAC CTTCGGAGAC 
AAGATCAAGG ACGACAACTT AATGCCTGCC TATTACAAAT GTATCCAGGA GGTGTTAAAA 
ACCTGGAGCC ACTGGTCCAT CCAAATTGTG GACGTGATTC CCTTTCTCAG GTTCTTCCCC 
AATCCAGGTC TCCGGAGGCT GAAGCAGGCC ATAGAGAAGA GGGATCACAT CGTGGAGATG 
CAGCTGAGGC AGCACAAGGA GAGCCTCGTG GCAGGCCAGT GGAGGGACAT GATGGACTAC 
ATGCTCCAAG GGGTGGCGCA GCCGAGCATG GAAGAGGGCT CTGGACAGCT CCTGGAAGGG 
CACGTGCACA TGGCTGCAGT GGACCTCCTG ATCGGTGGCA CTGAGACCAC AGCAAACACC 
CTCTCCTGGG CCGTGGTTTT TTTGCTTCAC CACCCTGAGA TTCAGCAGCG ACTGCAGGAG 
GAGCTAGACC ACGAACTGGG CCCTGGTGCC TCCAGCTCCC GGGTCCCCTA CAAGGACCGT 
GCACGGCTGC CCTTGCTCAA TGCCACCATC GCCGAGGTGC TGCGCCTGCG GCCCGTTGTG 
CCCTTAGCCT TGCCCCACCG CACCACACGG CCCAGCAGCA TCTCCGGCTA CGACATCCCT 
GAGGGCACAG TCATCATTCC GAACCTCCAA GGCGCCCACC TGGATGAGAC GGTCTGGGAG 
AGGCCACATG AGTTCTGGCC TGATCGCTTC CTGGAGCCAG GCAAGAACTC CAGAGCTCTG 
GCCTTCGGCT GCGGTGCCCG CGTGTGCCTG GGCGAGCCGC TGGCGCGCCT GGAGCTCTTC 
GTGGTGCTGA CCCGACTGCT GCAGGCCTTC ACGCTGCTGC CCTCCGGGGA CGCCCTGCCC 
TCCCTGCAGC CCCTGCCCCA CTGCAGTGTC ATCCTCAAGA TGCAGCCTTT CCAAGTGCGG 
CTGCAGCCCC GGGGGATGGG GGCCCACAGC CCGGGCCAGA GCCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000491.4
CDS	108..1595
Translation	MLLLGLLLLLPLLAGARLLWNWWKLRSLHLPPLAPGFLHLLQPDLPIYLLGLTQKFGPIYRLHLGLQDVVVLNSKRTIEEAMVKKWADFAGRPEPLTYKLVSRNYPDLSLGDYSLLWKAHKKLTRSALLLGIRDSMEPVVEQLTQEFCERMRAQPGTPVAIEEEFSLLTCSIICYLTFGDKIKDDNLMPAYYKCIQEVLKTWSHWSIQIVDVIPFLRFFPNPGLRRLKQAIEKRDHIVEMQLRQHKESLVAGQWRDMMDYMLQGVAQPSMEEGSGQLLEGHVHMAAVDLLIGGTETTANTLSWAVVFLLHHPEIQQRLQEELDHELGPGASSSRVPYKDRARLPLLNATIAEVLRLRPVVPLALPHRTTRPSSISGYDIPEGTVIIPNLQGAHLDETVWERPHEFWPDRFLEPGKNSRALAFGCGARVCLGEPLARLELFVVLTRLLQAFTLLPSGDALPSLQPLPHCSVILKMQPFQVRLQPRGMGAHSPGQSQ

Target ORF information:

RefSeq Version	NM_000500.7
Organism	Homo sapiens(human)
Definition	Homo sapiens cytochrome P450 family 21 subfamily A member 2 (CYP21A2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000500.7

ORF Insert Sequence:

ATGCTGCTCC TGGGCCTGCT GCTGCTGCTG CCCCTGCTGG CTGGCGCCCG CCTGCTGTGG 
AACTGGTGGA AGCTCCGGAG CCTCCACCTC CCGCCTCTTG CCCCGGGCTT CTTGCACCTG 
CTGCAGCCCG ACCTCCCCAT CTATCTGCTT GGCCTGACTC AGAAATTCGG GCCCATCTAC 
AGGCTCCACC TTGGGCTGCA AGATGTGGTG GTGCTGAACT CCAAGAGGAC CATTGAGGAA 
GCCATGGTCA AAAAGTGGGC AGACTTTGCT GGCAGACCTG AGCCACTTAC CTACAAGCTG 
GTGTCTAGGA ACTACCCGGA CCTGTCCTTG GGAGACTACT CCCTGCTCTG GAAAGCCCAC 
AAGAAGCTCA CCCGCTCAGC CCTGCTGCTG GGCATCCGTG ACTCCATGGA GCCAGTGGTG 
GAGCAGCTGA CCCAGGAGTT CTGTGAGCGC ATGAGAGCCC AGCCCGGCAC CCCTGTGGCC 
ATTGAGGAGG AATTCTCTCT CCTCACCTGC AGCATCATCT GTTACCTCAC CTTCGGAGAC 
AAGATCAAGG ACGACAACTT AATGCCTGCC TATTACAAAT GTATCCAGGA GGTGTTAAAA 
ACCTGGAGCC ACTGGTCCAT CCAAATTGTG GACGTGATTC CCTTTCTCAG GTTCTTCCCC 
AATCCAGGTC TCCGGAGGCT GAAGCAGGCC ATAGAGAAGA GGGATCACAT CGTGGAGATG 
CAGCTGAGGC AGCACAAGGA GAGCCTCGTG GCAGGCCAGT GGAGGGACAT GATGGACTAC 
ATGCTCCAAG GGGTGGCGCA GCCGAGCATG GAAGAGGGCT CTGGACAGCT CCTGGAAGGG 
CACGTGCACA TGGCTGCAGT GGACCTCCTG ATCGGTGGCA CTGAGACCAC AGCAAACACC 
CTCTCCTGGG CCGTGGTTTT TTTGCTTCAC CACCCTGAGA TTCAGCAGCG ACTGCAGGAG 
GAGCTAGACC ACGAACTGGG CCCTGGTGCC TCCAGCTCCC GGGTCCCCTA CAAGGACCGT 
GCACGGCTGC CCTTGCTCAA TGCCACCATC GCCGAGGTGC TGCGCCTGCG GCCCGTTGTG 
CCCTTAGCCT TGCCCCACCG CACCACACGG CCCAGCAGCA TCTCCGGCTA CGACATCCCT 
GAGGGCACAG TCATCATTCC GAACCTCCAA GGCGCCCACC TGGATGAGAC GGTCTGGGAG 
AGGCCACATG AGTTCTGGCC TGATCGCTTC CTGGAGCCAG GCAAGAACTC CAGAGCTCTG 
GCCTTCGGCT GCGGTGCCCG CGTGTGCCTG GGCGAGCCGC TGGCGCGCCT GGAGCTCTTC 
GTGGTGCTGA CCCGACTGCT GCAGGCCTTC ACGCTGCTGC CCTCCGGGGA CGCCCTGCCC 
TCCCTGCAGC CCCTGCCCCA CTGCAGTGTC ATCCTCAAGA TGCAGCCTTT CCAAGTGCGG 
CTGCAGCCCC GGGGGATGGG GGCCCACAGC CCGGGCCAGA GCCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CYP21A2 ( NM_000500.9 ) cDNA ORF clone, Homo sapiens(human) -> NP_000491.4 Homo sapiens cytochrome P450 family 21 subfamily A member 2 (CYP21A2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu21462
Clone ID Related Accession (Same CDS sequence)	NM_000500.7 , NM_000500.9
Accession Version	NM_000500.9 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1488bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-03
Organism	Homo sapiens(human)
Product	steroid 21-hydroxylase isoform a
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AV708092.1, DA008890.1, BC125181.1, AL645922.12, M17252.1 and AI479704.1. This sequence is a reference standard in the RefSeqGene project. On Aug 24, 2018 this sequence version replaced NM_000500.8. Transcript Variant: This variant (1) encodes the longer isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC125182.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145544, SAMEA2146411 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000644719.2/ ENSP00000496625.1 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGCTGCTCC TGGGCCTGCT GCTGCTGCTG CCCCTGCTGG CTGGCGCCCG CCTGCTGTGG 
AACTGGTGGA AGCTCCGGAG CCTCCACCTC CCGCCTCTTG CCCCGGGCTT CTTGCACCTG 
CTGCAGCCCG ACCTCCCCAT CTATCTGCTT GGCCTGACTC AGAAATTCGG GCCCATCTAC 
AGGCTCCACC TTGGGCTGCA AGATGTGGTG GTGCTGAACT CCAAGAGGAC CATTGAGGAA 
GCCATGGTCA AAAAGTGGGC AGACTTTGCT GGCAGACCTG AGCCACTTAC CTACAAGCTG 
GTGTCTAGGA ACTACCCGGA CCTGTCCTTG GGAGACTACT CCCTGCTCTG GAAAGCCCAC 
AAGAAGCTCA CCCGCTCAGC CCTGCTGCTG GGCATCCGTG ACTCCATGGA GCCAGTGGTG 
GAGCAGCTGA CCCAGGAGTT CTGTGAGCGC ATGAGAGCCC AGCCCGGCAC CCCTGTGGCC 
ATTGAGGAGG AATTCTCTCT CCTCACCTGC AGCATCATCT GTTACCTCAC CTTCGGAGAC 
AAGATCAAGG ACGACAACTT AATGCCTGCC TATTACAAAT GTATCCAGGA GGTGTTAAAA 
ACCTGGAGCC ACTGGTCCAT CCAAATTGTG GACGTGATTC CCTTTCTCAG GTTCTTCCCC 
AATCCAGGTC TCCGGAGGCT GAAGCAGGCC ATAGAGAAGA GGGATCACAT CGTGGAGATG 
CAGCTGAGGC AGCACAAGGA GAGCCTCGTG GCAGGCCAGT GGAGGGACAT GATGGACTAC 
ATGCTCCAAG GGGTGGCGCA GCCGAGCATG GAAGAGGGCT CTGGACAGCT CCTGGAAGGG 
CACGTGCACA TGGCTGCAGT GGACCTCCTG ATCGGTGGCA CTGAGACCAC AGCAAACACC 
CTCTCCTGGG CCGTGGTTTT TTTGCTTCAC CACCCTGAGA TTCAGCAGCG ACTGCAGGAG 
GAGCTAGACC ACGAACTGGG CCCTGGTGCC TCCAGCTCCC GGGTCCCCTA CAAGGACCGT 
GCACGGCTGC CCTTGCTCAA TGCCACCATC GCCGAGGTGC TGCGCCTGCG GCCCGTTGTG 
CCCTTAGCCT TGCCCCACCG CACCACACGG CCCAGCAGCA TCTCCGGCTA CGACATCCCT 
GAGGGCACAG TCATCATTCC GAACCTCCAA GGCGCCCACC TGGATGAGAC GGTCTGGGAG 
AGGCCACATG AGTTCTGGCC TGATCGCTTC CTGGAGCCAG GCAAGAACTC CAGAGCTCTG 
GCCTTCGGCT GCGGTGCCCG CGTGTGCCTG GGCGAGCCGC TGGCGCGCCT GGAGCTCTTC 
GTGGTGCTGA CCCGACTGCT GCAGGCCTTC ACGCTGCTGC CCTCCGGGGA CGCCCTGCCC 
TCCCTGCAGC CCCTGCCCCA CTGCAGTGTC ATCCTCAAGA TGCAGCCTTT CCAAGTGCGG 
CTGCAGCCCC GGGGGATGGG GGCCCACAGC CCGGGCCAGA GCCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000491.4
CDS	9..1496
Translation	MLLLGLLLLLPLLAGARLLWNWWKLRSLHLPPLAPGFLHLLQPDLPIYLLGLTQKFGPIYRLHLGLQDVVVLNSKRTIEEAMVKKWADFAGRPEPLTYKLVSRNYPDLSLGDYSLLWKAHKKLTRSALLLGIRDSMEPVVEQLTQEFCERMRAQPGTPVAIEEEFSLLTCSIICYLTFGDKIKDDNLMPAYYKCIQEVLKTWSHWSIQIVDVIPFLRFFPNPGLRRLKQAIEKRDHIVEMQLRQHKESLVAGQWRDMMDYMLQGVAQPSMEEGSGQLLEGHVHMAAVDLLIGGTETTANTLSWAVVFLLHHPEIQQRLQEELDHELGPGASSSRVPYKDRARLPLLNATIAEVLRLRPVVPLALPHRTTRPSSISGYDIPEGTVIIPNLQGAHLDETVWERPHEFWPDRFLEPGKNSRALAFGCGARVCLGEPLARLELFVVLTRLLQAFTLLPSGDALPSLQPLPHCSVILKMQPFQVRLQPRGMGAHSPGQSQ

Target ORF information:

RefSeq Version	NM_000500.9
Organism	Homo sapiens(human)
Definition	Homo sapiens cytochrome P450 family 21 subfamily A member 2 (CYP21A2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000500.9

ORF Insert Sequence:

ATGCTGCTCC TGGGCCTGCT GCTGCTGCTG CCCCTGCTGG CTGGCGCCCG CCTGCTGTGG 
AACTGGTGGA AGCTCCGGAG CCTCCACCTC CCGCCTCTTG CCCCGGGCTT CTTGCACCTG 
CTGCAGCCCG ACCTCCCCAT CTATCTGCTT GGCCTGACTC AGAAATTCGG GCCCATCTAC 
AGGCTCCACC TTGGGCTGCA AGATGTGGTG GTGCTGAACT CCAAGAGGAC CATTGAGGAA 
GCCATGGTCA AAAAGTGGGC AGACTTTGCT GGCAGACCTG AGCCACTTAC CTACAAGCTG 
GTGTCTAGGA ACTACCCGGA CCTGTCCTTG GGAGACTACT CCCTGCTCTG GAAAGCCCAC 
AAGAAGCTCA CCCGCTCAGC CCTGCTGCTG GGCATCCGTG ACTCCATGGA GCCAGTGGTG 
GAGCAGCTGA CCCAGGAGTT CTGTGAGCGC ATGAGAGCCC AGCCCGGCAC CCCTGTGGCC 
ATTGAGGAGG AATTCTCTCT CCTCACCTGC AGCATCATCT GTTACCTCAC CTTCGGAGAC 
AAGATCAAGG ACGACAACTT AATGCCTGCC TATTACAAAT GTATCCAGGA GGTGTTAAAA 
ACCTGGAGCC ACTGGTCCAT CCAAATTGTG GACGTGATTC CCTTTCTCAG GTTCTTCCCC 
AATCCAGGTC TCCGGAGGCT GAAGCAGGCC ATAGAGAAGA GGGATCACAT CGTGGAGATG 
CAGCTGAGGC AGCACAAGGA GAGCCTCGTG GCAGGCCAGT GGAGGGACAT GATGGACTAC 
ATGCTCCAAG GGGTGGCGCA GCCGAGCATG GAAGAGGGCT CTGGACAGCT CCTGGAAGGG 
CACGTGCACA TGGCTGCAGT GGACCTCCTG ATCGGTGGCA CTGAGACCAC AGCAAACACC 
CTCTCCTGGG CCGTGGTTTT TTTGCTTCAC CACCCTGAGA TTCAGCAGCG ACTGCAGGAG 
GAGCTAGACC ACGAACTGGG CCCTGGTGCC TCCAGCTCCC GGGTCCCCTA CAAGGACCGT 
GCACGGCTGC CCTTGCTCAA TGCCACCATC GCCGAGGTGC TGCGCCTGCG GCCCGTTGTG 
CCCTTAGCCT TGCCCCACCG CACCACACGG CCCAGCAGCA TCTCCGGCTA CGACATCCCT 
GAGGGCACAG TCATCATTCC GAACCTCCAA GGCGCCCACC TGGATGAGAC GGTCTGGGAG 
AGGCCACATG AGTTCTGGCC TGATCGCTTC CTGGAGCCAG GCAAGAACTC CAGAGCTCTG 
GCCTTCGGCT GCGGTGCCCG CGTGTGCCTG GGCGAGCCGC TGGCGCGCCT GGAGCTCTTC 
GTGGTGCTGA CCCGACTGCT GCAGGCCTTC ACGCTGCTGC CCTCCGGGGA CGCCCTGCCC 
TCCCTGCAGC CCCTGCCCCA CTGCAGTGTC ATCCTCAAGA TGCAGCCTTT CCAAGTGCGG 
CTGCAGCCCC GGGGGATGGG GGCCCACAGC CCGGGCCAGA GCCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CYP21A2 ( NM_001128590.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001122062.3 Homo sapiens cytochrome P450 family 21 subfamily A member 2 (CYP21A2), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu21027
Clone ID Related Accession (Same CDS sequence)	NM_001128590.3
Accession Version	NM_001128590.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1398bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-03
Organism	Homo sapiens(human)
Product	steroid 21-hydroxylase isoform b
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AV708092.1, DA008890.1, AK054616.1, BC125181.1, CD013985.1, M17252.1 and AL645922.12. On Feb 17, 2011 this sequence version replaced NM_001128590.2. Transcript Variant: This variant (2) lacks an alternate in-frame exon compared to variant 1. The resulting isoform (b) has the same N- and C-termini but is shorter compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA2145544, SAMEA2146411 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

ATGCTGCTCC TGGGCCTGCT GCTGCTGCTG CCCCTGCTGG CTGGCGCCCG CCTGCTGTGG 
AACTGGTGGA AGCTCCGGAG CCTCCACCTC CCGCCTCTTG CCCCGGGCTT CTTGCACCTG 
CTGCAGCCCG ACCTCCCCAT CTATCTGCTT GGCCTGACTC AGAAATTCGG GCCCATCTAC 
AGGCTCCACC TTGGGCTGCA AGACAAGCTG GTGTCTAGGA ACTACCCGGA CCTGTCCTTG 
GGAGACTACT CCCTGCTCTG GAAAGCCCAC AAGAAGCTCA CCCGCTCAGC CCTGCTGCTG 
GGCATCCGTG ACTCCATGGA GCCAGTGGTG GAGCAGCTGA CCCAGGAGTT CTGTGAGCGC 
ATGAGAGCCC AGCCCGGCAC CCCTGTGGCC ATTGAGGAGG AATTCTCTCT CCTCACCTGC 
AGCATCATCT GTTACCTCAC CTTCGGAGAC AAGATCAAGG ACGACAACTT AATGCCTGCC 
TATTACAAAT GTATCCAGGA GGTGTTAAAA ACCTGGAGCC ACTGGTCCAT CCAAATTGTG 
GACGTGATTC CCTTTCTCAG GTTCTTCCCC AATCCAGGTC TCCGGAGGCT GAAGCAGGCC 
ATAGAGAAGA GGGATCACAT CGTGGAGATG CAGCTGAGGC AGCACAAGGA GAGCCTCGTG 
GCAGGCCAGT GGAGGGACAT GATGGACTAC ATGCTCCAAG GGGTGGCGCA GCCGAGCATG 
GAAGAGGGCT CTGGACAGCT CCTGGAAGGG CACGTGCACA TGGCTGCAGT GGACCTCCTG 
ATCGGTGGCA CTGAGACCAC AGCAAACACC CTCTCCTGGG CCGTGGTTTT TTTGCTTCAC 
CACCCTGAGA TTCAGCAGCG ACTGCAGGAG GAGCTAGACC ACGAACTGGG CCCTGGTGCC 
TCCAGCTCCC GGGTCCCCTA CAAGGACCGT GCACGGCTGC CCTTGCTCAA TGCCACCATC 
GCCGAGGTGC TGCGCCTGCG GCCCGTTGTG CCCTTAGCCT TGCCCCACCG CACCACACGG 
CCCAGCAGCA TCTCCGGCTA CGACATCCCT GAGGGCACAG TCATCATTCC GAACCTCCAA 
GGCGCCCACC TGGATGAGAC GGTCTGGGAG AGGCCACATG AGTTCTGGCC TGATCGCTTC 
CTGGAGCCAG GCAAGAACTC CAGAGCTCTG GCCTTCGGCT GCGGTGCCCG CGTGTGCCTG 
GGCGAGCCGC TGGCGCGCCT GGAGCTCTTC GTGGTGCTGA CCCGACTGCT GCAGGCCTTC 
ACGCTGCTGC CCTCCGGGGA CGCCCTGCCC TCCCTGCAGC CCCTGCCCCA CTGCAGTGTC 
ATCCTCAAGA TGCAGCCTTT CCAAGTGCGG CTGCAGCCCC GGGGGATGGG GGCCCACAGC 
CCGGGCCAGA GCCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001122062.3
CDS	108..1505
Translation	MLLLGLLLLLPLLAGARLLWNWWKLRSLHLPPLAPGFLHLLQPDLPIYLLGLTQKFGPIYRLHLGLQDKLVSRNYPDLSLGDYSLLWKAHKKLTRSALLLGIRDSMEPVVEQLTQEFCERMRAQPGTPVAIEEEFSLLTCSIICYLTFGDKIKDDNLMPAYYKCIQEVLKTWSHWSIQIVDVIPFLRFFPNPGLRRLKQAIEKRDHIVEMQLRQHKESLVAGQWRDMMDYMLQGVAQPSMEEGSGQLLEGHVHMAAVDLLIGGTETTANTLSWAVVFLLHHPEIQQRLQEELDHELGPGASSSRVPYKDRARLPLLNATIAEVLRLRPVVPLALPHRTTRPSSISGYDIPEGTVIIPNLQGAHLDETVWERPHEFWPDRFLEPGKNSRALAFGCGARVCLGEPLARLELFVVLTRLLQAFTLLPSGDALPSLQPLPHCSVILKMQPFQVRLQPRGMGAHSPGQSQ

Target ORF information:

RefSeq Version	NM_001128590.3
Organism	Homo sapiens(human)
Definition	Homo sapiens cytochrome P450 family 21 subfamily A member 2 (CYP21A2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001128590.3

ORF Insert Sequence:

ATGCTGCTCC TGGGCCTGCT GCTGCTGCTG CCCCTGCTGG CTGGCGCCCG CCTGCTGTGG 
AACTGGTGGA AGCTCCGGAG CCTCCACCTC CCGCCTCTTG CCCCGGGCTT CTTGCACCTG 
CTGCAGCCCG ACCTCCCCAT CTATCTGCTT GGCCTGACTC AGAAATTCGG GCCCATCTAC 
AGGCTCCACC TTGGGCTGCA AGACAAGCTG GTGTCTAGGA ACTACCCGGA CCTGTCCTTG 
GGAGACTACT CCCTGCTCTG GAAAGCCCAC AAGAAGCTCA CCCGCTCAGC CCTGCTGCTG 
GGCATCCGTG ACTCCATGGA GCCAGTGGTG GAGCAGCTGA CCCAGGAGTT CTGTGAGCGC 
ATGAGAGCCC AGCCCGGCAC CCCTGTGGCC ATTGAGGAGG AATTCTCTCT CCTCACCTGC 
AGCATCATCT GTTACCTCAC CTTCGGAGAC AAGATCAAGG ACGACAACTT AATGCCTGCC 
TATTACAAAT GTATCCAGGA GGTGTTAAAA ACCTGGAGCC ACTGGTCCAT CCAAATTGTG 
GACGTGATTC CCTTTCTCAG GTTCTTCCCC AATCCAGGTC TCCGGAGGCT GAAGCAGGCC 
ATAGAGAAGA GGGATCACAT CGTGGAGATG CAGCTGAGGC AGCACAAGGA GAGCCTCGTG 
GCAGGCCAGT GGAGGGACAT GATGGACTAC ATGCTCCAAG GGGTGGCGCA GCCGAGCATG 
GAAGAGGGCT CTGGACAGCT CCTGGAAGGG CACGTGCACA TGGCTGCAGT GGACCTCCTG 
ATCGGTGGCA CTGAGACCAC AGCAAACACC CTCTCCTGGG CCGTGGTTTT TTTGCTTCAC 
CACCCTGAGA TTCAGCAGCG ACTGCAGGAG GAGCTAGACC ACGAACTGGG CCCTGGTGCC 
TCCAGCTCCC GGGTCCCCTA CAAGGACCGT GCACGGCTGC CCTTGCTCAA TGCCACCATC 
GCCGAGGTGC TGCGCCTGCG GCCCGTTGTG CCCTTAGCCT TGCCCCACCG CACCACACGG 
CCCAGCAGCA TCTCCGGCTA CGACATCCCT GAGGGCACAG TCATCATTCC GAACCTCCAA 
GGCGCCCACC TGGATGAGAC GGTCTGGGAG AGGCCACATG AGTTCTGGCC TGATCGCTTC 
CTGGAGCCAG GCAAGAACTC CAGAGCTCTG GCCTTCGGCT GCGGTGCCCG CGTGTGCCTG 
GGCGAGCCGC TGGCGCGCCT GGAGCTCTTC GTGGTGCTGA CCCGACTGCT GCAGGCCTTC 
ACGCTGCTGC CCTCCGGGGA CGCCCTGCCC TCCCTGCAGC CCCTGCCCCA CTGCAGTGTC 
ATCCTCAAGA TGCAGCCTTT CCAAGTGCGG CTGCAGCCCC GGGGGATGGG GGCCCACAGC 
CCGGGCCAGA GCCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CYP21A2 ( NM_001368143.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001355072.1 Homo sapiens cytochrome P450 family 21 subfamily A member 2 (CYP21A2), transcript variant 3, mRNA.

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CloneID	OHu57180
Clone ID Related Accession (Same CDS sequence)	NM_001368143.1 , XM_024452557.1 , NM_001368144.1
Accession Version	NM_001368143.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1083bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-03
Organism	Homo sapiens(human)
Product	steroid 21-hydroxylase isoform c
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL645922.12. Transcript Variant: This variant (3), as well as variant 4, encodes isoform c. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA2145544, SAMEA2146411 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

ATGGAGCCAG TGGTGGAGCA GCTGACCCAG GAGTTCTGTG AGCGCATGAG AGCCCAGCCC 
GGCACCCCTG TGGCCATTGA GGAGGAATTC TCTCTCCTCA CCTGCAGCAT CATCTGTTAC 
CTCACCTTCG GAGACAAGAT CAAGGACGAC AACTTAATGC CTGCCTATTA CAAATGTATC 
CAGGAGGTGT TAAAAACCTG GAGCCACTGG TCCATCCAAA TTGTGGACGT GATTCCCTTT 
CTCAGGTTCT TCCCCAATCC AGGTCTCCGG AGGCTGAAGC AGGCCATAGA GAAGAGGGAT 
CACATCGTGG AGATGCAGCT GAGGCAGCAC AAGGAGAGCC TCGTGGCAGG CCAGTGGAGG 
GACATGATGG ACTACATGCT CCAAGGGGTG GCGCAGCCGA GCATGGAAGA GGGCTCTGGA 
CAGCTCCTGG AAGGGCACGT GCACATGGCT GCAGTGGACC TCCTGATCGG TGGCACTGAG 
ACCACAGCAA ACACCCTCTC CTGGGCCGTG GTTTTTTTGC TTCACCACCC TGAGATTCAG 
CAGCGACTGC AGGAGGAGCT AGACCACGAA CTGGGCCCTG GTGCCTCCAG CTCCCGGGTC 
CCCTACAAGG ACCGTGCACG GCTGCCCTTG CTCAATGCCA CCATCGCCGA GGTGCTGCGC 
CTGCGGCCCG TTGTGCCCTT AGCCTTGCCC CACCGCACCA CACGGCCCAG CAGCATCTCC 
GGCTACGACA TCCCTGAGGG CACAGTCATC ATTCCGAACC TCCAAGGCGC CCACCTGGAT 
GAGACGGTCT GGGAGAGGCC ACATGAGTTC TGGCCTGATC GCTTCCTGGA GCCAGGCAAG 
AACTCCAGAG CTCTGGCCTT CGGCTGCGGT GCCCGCGTGT GCCTGGGCGA GCCGCTGGCG 
CGCCTGGAGC TCTTCGTGGT GCTGACCCGA CTGCTGCAGG CCTTCACGCT GCTGCCCTCC 
GGGGACGCCC TGCCCTCCCT GCAGCCCCTG CCCCACTGCA GTGTCATCCT CAAGATGCAG 
CCTTTCCAAG TGCGGCTGCA GCCCCGGGGG ATGGGGGCCC ACAGCCCGGG CCAGAGCCAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001355072.1
CDS	542..1624
Translation	MEPVVEQLTQEFCERMRAQPGTPVAIEEEFSLLTCSIICYLTFGDKIKDDNLMPAYYKCIQEVLKTWSHWSIQIVDVIPFLRFFPNPGLRRLKQAIEKRDHIVEMQLRQHKESLVAGQWRDMMDYMLQGVAQPSMEEGSGQLLEGHVHMAAVDLLIGGTETTANTLSWAVVFLLHHPEIQQRLQEELDHELGPGASSSRVPYKDRARLPLLNATIAEVLRLRPVVPLALPHRTTRPSSISGYDIPEGTVIIPNLQGAHLDETVWERPHEFWPDRFLEPGKNSRALAFGCGARVCLGEPLARLELFVVLTRLLQAFTLLPSGDALPSLQPLPHCSVILKMQPFQVRLQPRGMGAHSPGQSQ

Target ORF information:

RefSeq Version	NM_001368143.1
Organism	Homo sapiens(human)
Definition	Homo sapiens cytochrome P450 family 21 subfamily A member 2 (CYP21A2), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001368143.1

ORF Insert Sequence:

ATGGAGCCAG TGGTGGAGCA GCTGACCCAG GAGTTCTGTG AGCGCATGAG AGCCCAGCCC 
GGCACCCCTG TGGCCATTGA GGAGGAATTC TCTCTCCTCA CCTGCAGCAT CATCTGTTAC 
CTCACCTTCG GAGACAAGAT CAAGGACGAC AACTTAATGC CTGCCTATTA CAAATGTATC 
CAGGAGGTGT TAAAAACCTG GAGCCACTGG TCCATCCAAA TTGTGGACGT GATTCCCTTT 
CTCAGGTTCT TCCCCAATCC AGGTCTCCGG AGGCTGAAGC AGGCCATAGA GAAGAGGGAT 
CACATCGTGG AGATGCAGCT GAGGCAGCAC AAGGAGAGCC TCGTGGCAGG CCAGTGGAGG 
GACATGATGG ACTACATGCT CCAAGGGGTG GCGCAGCCGA GCATGGAAGA GGGCTCTGGA 
CAGCTCCTGG AAGGGCACGT GCACATGGCT GCAGTGGACC TCCTGATCGG TGGCACTGAG 
ACCACAGCAA ACACCCTCTC CTGGGCCGTG GTTTTTTTGC TTCACCACCC TGAGATTCAG 
CAGCGACTGC AGGAGGAGCT AGACCACGAA CTGGGCCCTG GTGCCTCCAG CTCCCGGGTC 
CCCTACAAGG ACCGTGCACG GCTGCCCTTG CTCAATGCCA CCATCGCCGA GGTGCTGCGC 
CTGCGGCCCG TTGTGCCCTT AGCCTTGCCC CACCGCACCA CACGGCCCAG CAGCATCTCC 
GGCTACGACA TCCCTGAGGG CACAGTCATC ATTCCGAACC TCCAAGGCGC CCACCTGGAT 
GAGACGGTCT GGGAGAGGCC ACATGAGTTC TGGCCTGATC GCTTCCTGGA GCCAGGCAAG 
AACTCCAGAG CTCTGGCCTT CGGCTGCGGT GCCCGCGTGT GCCTGGGCGA GCCGCTGGCG 
CGCCTGGAGC TCTTCGTGGT GCTGACCCGA CTGCTGCAGG CCTTCACGCT GCTGCCCTCC 
GGGGACGCCC TGCCCTCCCT GCAGCCCCTG CCCCACTGCA GTGTCATCCT CAAGATGCAG 
CCTTTCCAAG TGCGGCTGCA GCCCCGGGGG ATGGGGGCCC ACAGCCCGGG CCAGAGCCAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CYP21A2 ( NM_001368144.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001355073.1 Homo sapiens cytochrome P450 family 21 subfamily A member 2 (CYP21A2), transcript variant 4, mRNA.

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CloneID	OHu57180
Clone ID Related Accession (Same CDS sequence)	NM_001368143.1 , XM_024452557.1 , NM_001368144.1
Accession Version	NM_001368144.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1083bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-01
Organism	Homo sapiens(human)
Product	steroid 21-hydroxylase isoform c
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL645922.12. Transcript Variant: This variant (4), as well as variant 3, encodes isoform c. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA2145544, SAMEA2146411 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

ATGGAGCCAG TGGTGGAGCA GCTGACCCAG GAGTTCTGTG AGCGCATGAG AGCCCAGCCC 
GGCACCCCTG TGGCCATTGA GGAGGAATTC TCTCTCCTCA CCTGCAGCAT CATCTGTTAC 
CTCACCTTCG GAGACAAGAT CAAGGACGAC AACTTAATGC CTGCCTATTA CAAATGTATC 
CAGGAGGTGT TAAAAACCTG GAGCCACTGG TCCATCCAAA TTGTGGACGT GATTCCCTTT 
CTCAGGTTCT TCCCCAATCC AGGTCTCCGG AGGCTGAAGC AGGCCATAGA GAAGAGGGAT 
CACATCGTGG AGATGCAGCT GAGGCAGCAC AAGGAGAGCC TCGTGGCAGG CCAGTGGAGG 
GACATGATGG ACTACATGCT CCAAGGGGTG GCGCAGCCGA GCATGGAAGA GGGCTCTGGA 
CAGCTCCTGG AAGGGCACGT GCACATGGCT GCAGTGGACC TCCTGATCGG TGGCACTGAG 
ACCACAGCAA ACACCCTCTC CTGGGCCGTG GTTTTTTTGC TTCACCACCC TGAGATTCAG 
CAGCGACTGC AGGAGGAGCT AGACCACGAA CTGGGCCCTG GTGCCTCCAG CTCCCGGGTC 
CCCTACAAGG ACCGTGCACG GCTGCCCTTG CTCAATGCCA CCATCGCCGA GGTGCTGCGC 
CTGCGGCCCG TTGTGCCCTT AGCCTTGCCC CACCGCACCA CACGGCCCAG CAGCATCTCC 
GGCTACGACA TCCCTGAGGG CACAGTCATC ATTCCGAACC TCCAAGGCGC CCACCTGGAT 
GAGACGGTCT GGGAGAGGCC ACATGAGTTC TGGCCTGATC GCTTCCTGGA GCCAGGCAAG 
AACTCCAGAG CTCTGGCCTT CGGCTGCGGT GCCCGCGTGT GCCTGGGCGA GCCGCTGGCG 
CGCCTGGAGC TCTTCGTGGT GCTGACCCGA CTGCTGCAGG CCTTCACGCT GCTGCCCTCC 
GGGGACGCCC TGCCCTCCCT GCAGCCCCTG CCCCACTGCA GTGTCATCCT CAAGATGCAG 
CCTTTCCAAG TGCGGCTGCA GCCCCGGGGG ATGGGGGCCC ACAGCCCGGG CCAGAGCCAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001355073.1
CDS	452..1534
Translation	MEPVVEQLTQEFCERMRAQPGTPVAIEEEFSLLTCSIICYLTFGDKIKDDNLMPAYYKCIQEVLKTWSHWSIQIVDVIPFLRFFPNPGLRRLKQAIEKRDHIVEMQLRQHKESLVAGQWRDMMDYMLQGVAQPSMEEGSGQLLEGHVHMAAVDLLIGGTETTANTLSWAVVFLLHHPEIQQRLQEELDHELGPGASSSRVPYKDRARLPLLNATIAEVLRLRPVVPLALPHRTTRPSSISGYDIPEGTVIIPNLQGAHLDETVWERPHEFWPDRFLEPGKNSRALAFGCGARVCLGEPLARLELFVVLTRLLQAFTLLPSGDALPSLQPLPHCSVILKMQPFQVRLQPRGMGAHSPGQSQ

Target ORF information:

RefSeq Version	NM_001368144.1
Organism	Homo sapiens(human)
Definition	Homo sapiens cytochrome P450 family 21 subfamily A member 2 (CYP21A2), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001368144.1

ORF Insert Sequence:

ATGGAGCCAG TGGTGGAGCA GCTGACCCAG GAGTTCTGTG AGCGCATGAG AGCCCAGCCC 
GGCACCCCTG TGGCCATTGA GGAGGAATTC TCTCTCCTCA CCTGCAGCAT CATCTGTTAC 
CTCACCTTCG GAGACAAGAT CAAGGACGAC AACTTAATGC CTGCCTATTA CAAATGTATC 
CAGGAGGTGT TAAAAACCTG GAGCCACTGG TCCATCCAAA TTGTGGACGT GATTCCCTTT 
CTCAGGTTCT TCCCCAATCC AGGTCTCCGG AGGCTGAAGC AGGCCATAGA GAAGAGGGAT 
CACATCGTGG AGATGCAGCT GAGGCAGCAC AAGGAGAGCC TCGTGGCAGG CCAGTGGAGG 
GACATGATGG ACTACATGCT CCAAGGGGTG GCGCAGCCGA GCATGGAAGA GGGCTCTGGA 
CAGCTCCTGG AAGGGCACGT GCACATGGCT GCAGTGGACC TCCTGATCGG TGGCACTGAG 
ACCACAGCAA ACACCCTCTC CTGGGCCGTG GTTTTTTTGC TTCACCACCC TGAGATTCAG 
CAGCGACTGC AGGAGGAGCT AGACCACGAA CTGGGCCCTG GTGCCTCCAG CTCCCGGGTC 
CCCTACAAGG ACCGTGCACG GCTGCCCTTG CTCAATGCCA CCATCGCCGA GGTGCTGCGC 
CTGCGGCCCG TTGTGCCCTT AGCCTTGCCC CACCGCACCA CACGGCCCAG CAGCATCTCC 
GGCTACGACA TCCCTGAGGG CACAGTCATC ATTCCGAACC TCCAAGGCGC CCACCTGGAT 
GAGACGGTCT GGGAGAGGCC ACATGAGTTC TGGCCTGATC GCTTCCTGGA GCCAGGCAAG 
AACTCCAGAG CTCTGGCCTT CGGCTGCGGT GCCCGCGTGT GCCTGGGCGA GCCGCTGGCG 
CGCCTGGAGC TCTTCGTGGT GCTGACCCGA CTGCTGCAGG CCTTCACGCT GCTGCCCTCC 
GGGGACGCCC TGCCCTCCCT GCAGCCCCTG CCCCACTGCA GTGTCATCCT CAAGATGCAG 
CCTTTCCAAG TGCGGCTGCA GCCCCGGGGG ATGGGGGCCC ACAGCCCGGG CCAGAGCCAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CYP21A2 ( XM_024452557.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024308325.1 Homo sapiens cytochrome P450 family 21 subfamily A member 2 (CYP21A2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHu57180
Clone ID Related Accession (Same CDS sequence)	NM_001368143.1 , XM_024452557.1 , NM_001368144.1
Accession Version	XM_024452557.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1083bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	steroid 21-hydroxylase isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_167248.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCCAG TGGTGGAGCA GCTGACCCAG GAGTTCTGTG AGCGCATGAG AGCCCAGCCC 
GGCACCCCTG TGGCCATTGA GGAGGAATTC TCTCTCCTCA CCTGCAGCAT CATCTGTTAC 
CTCACCTTCG GAGACAAGAT CAAGGACGAC AACTTAATGC CTGCCTATTA CAAATGTATC 
CAGGAGGTGT TAAAAACCTG GAGCCACTGG TCCATCCAAA TTGTGGACGT GATTCCCTTT 
CTCAGGTTCT TCCCCAATCC AGGTCTCCGG AGGCTGAAGC AGGCCATAGA GAAGAGGGAT 
CACATCGTGG AGATGCAGCT GAGGCAGCAC AAGGAGAGCC TCGTGGCAGG CCAGTGGAGG 
GACATGATGG ACTACATGCT CCAAGGGGTG GCGCAGCCGA GCATGGAAGA GGGCTCTGGA 
CAGCTCCTGG AAGGGCACGT GCACATGGCT GCAGTGGACC TCCTGATCGG TGGCACTGAG 
ACCACAGCAA ACACCCTCTC CTGGGCCGTG GTTTTTTTGC TTCACCACCC TGAGATTCAG 
CAGCGACTGC AGGAGGAGCT AGACCACGAA CTGGGCCCTG GTGCCTCCAG CTCCCGGGTC 
CCCTACAAGG ACCGTGCACG GCTGCCCTTG CTCAATGCCA CCATCGCCGA GGTGCTGCGC 
CTGCGGCCCG TTGTGCCCTT AGCCTTGCCC CACCGCACCA CACGGCCCAG CAGCATCTCC 
GGCTACGACA TCCCTGAGGG CACAGTCATC ATTCCGAACC TCCAAGGCGC CCACCTGGAT 
GAGACGGTCT GGGAGAGGCC ACATGAGTTC TGGCCTGATC GCTTCCTGGA GCCAGGCAAG 
AACTCCAGAG CTCTGGCCTT CGGCTGCGGT GCCCGCGTGT GCCTGGGCGA GCCGCTGGCG 
CGCCTGGAGC TCTTCGTGGT GCTGACCCGA CTGCTGCAGG CCTTCACGCT GCTGCCCTCC 
GGGGACGCCC TGCCCTCCCT GCAGCCCCTG CCCCACTGCA GTGTCATCCT CAAGATGCAG 
CCTTTCCAAG TGCGGCTGCA GCCCCGGGGG ATGGGGGCCC ACAGCCCGGG CCAGAGCCAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024308325.1
CDS	506..1588
Translation	MEPVVEQLTQEFCERMRAQPGTPVAIEEEFSLLTCSIICYLTFGDKIKDDNLMPAYYKCIQEVLKTWSHWSIQIVDVIPFLRFFPNPGLRRLKQAIEKRDHIVEMQLRQHKESLVAGQWRDMMDYMLQGVAQPSMEEGSGQLLEGHVHMAAVDLLIGGTETTANTLSWAVVFLLHHPEIQQRLQEELDHELGPGASSSRVPYKDRARLPLLNATIAEVLRLRPVVPLALPHRTTRPSSISGYDIPEGTVIIPNLQGAHLDETVWERPHEFWPDRFLEPGKNSRALAFGCGARVCLGEPLARLELFVVLTRLLQAFTLLPSGDALPSLQPLPHCSVILKMQPFQVRLQPRGMGAHSPGQSQ

Target ORF information:

RefSeq Version	XM_024452557.1
Organism	Homo sapiens(human)
Definition	Homo sapiens cytochrome P450 family 21 subfamily A member 2 (CYP21A2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024452557.1

ORF Insert Sequence:

ATGGAGCCAG TGGTGGAGCA GCTGACCCAG GAGTTCTGTG AGCGCATGAG AGCCCAGCCC 
GGCACCCCTG TGGCCATTGA GGAGGAATTC TCTCTCCTCA CCTGCAGCAT CATCTGTTAC 
CTCACCTTCG GAGACAAGAT CAAGGACGAC AACTTAATGC CTGCCTATTA CAAATGTATC 
CAGGAGGTGT TAAAAACCTG GAGCCACTGG TCCATCCAAA TTGTGGACGT GATTCCCTTT 
CTCAGGTTCT TCCCCAATCC AGGTCTCCGG AGGCTGAAGC AGGCCATAGA GAAGAGGGAT 
CACATCGTGG AGATGCAGCT GAGGCAGCAC AAGGAGAGCC TCGTGGCAGG CCAGTGGAGG 
GACATGATGG ACTACATGCT CCAAGGGGTG GCGCAGCCGA GCATGGAAGA GGGCTCTGGA 
CAGCTCCTGG AAGGGCACGT GCACATGGCT GCAGTGGACC TCCTGATCGG TGGCACTGAG 
ACCACAGCAA ACACCCTCTC CTGGGCCGTG GTTTTTTTGC TTCACCACCC TGAGATTCAG 
CAGCGACTGC AGGAGGAGCT AGACCACGAA CTGGGCCCTG GTGCCTCCAG CTCCCGGGTC 
CCCTACAAGG ACCGTGCACG GCTGCCCTTG CTCAATGCCA CCATCGCCGA GGTGCTGCGC 
CTGCGGCCCG TTGTGCCCTT AGCCTTGCCC CACCGCACCA CACGGCCCAG CAGCATCTCC 
GGCTACGACA TCCCTGAGGG CACAGTCATC ATTCCGAACC TCCAAGGCGC CCACCTGGAT 
GAGACGGTCT GGGAGAGGCC ACATGAGTTC TGGCCTGATC GCTTCCTGGA GCCAGGCAAG 
AACTCCAGAG CTCTGGCCTT CGGCTGCGGT GCCCGCGTGT GCCTGGGCGA GCCGCTGGCG 
CGCCTGGAGC TCTTCGTGGT GCTGACCCGA CTGCTGCAGG CCTTCACGCT GCTGCCCTCC 
GGGGACGCCC TGCCCTCCCT GCAGCCCCTG CCCCACTGCA GTGTCATCCT CAAGATGCAG 
CCTTTCCAAG TGCGGCTGCA GCCCCGGGGG ATGGGGGCCC ACAGCCCGGG CCAGAGCCAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CYP21A2 ( XM_024452555.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024308323.1 Homo sapiens cytochrome P450 family 21 subfamily A member 2 (CYP21A2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu106316
Clone ID Related Accession (Same CDS sequence)	XM_024452555.1
Accession Version	XM_024452555.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1488bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	steroid 21-hydroxylase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_167248.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCTCC TGGGCCTGCT GCTGCTGCTG CCCCTGCTGG CTGGCGCCCG CCTGCTGTGG 
AACTGGTGGA AGCTCCGGAG CCTCCACCTC CCGCCTCTTG CCCCGGGCTT CTTGCACTTG 
CTGCAGCCCG ACCTCCCAAT CTATCTGCTT GGCCTGACTC AGAAATTCGG GCCCATCTAC 
AGGCTCCACC TTGGGCTGCA AGATGTGGTG GTGCTGAACT CCAAGAGGAC CATTGAGGAA 
GCCATGGTCA AAAAGTGGGC AGACTTTGCT GGCAGACCTG AGCCACTTAC CTACAAGCTG 
GTGTCTAGGA ACTACCCGGA CCTGTCCTTG GGAGACTACT CCCTGCTCTG GAAAGCCCAC 
AAGAAGCTCA CCCGCTCAGC CCTGCTGCTG GGCATCCGTG ACTCCATGGA GCCAGTGGTG 
GAGCAGCTGA CCCAGGAGTT CTGTGAGCGC ATGAGAGCCC AGCCCGGCAC CCCTGTGGCC 
ATTGAGGAGG AATTCTCTCT CCTCACCTGC AGCATCATCT GTTACCTCAC CTTCGGAGAC 
AAGATCAAGG ACGACAACTT AATGCCTGCC TATTACAAAT GTATCCAGGA GGTGTTAAAA 
ACCTGGAGCC ACTGGTCCAT CCAAATTGTG GACGTGATTC CCTTTCTCAG GTTCTTCCCC 
AATCCAGGTC TCCGGAGGCT GAAGCAGGCC ATAGAGAAGA GGGATCACAT CGTGGAGATG 
CAGCTGAGGC AGCACAAGGA GAGCCTCGTG GCAGGCCAGT GGAGGGACAT GATGGACTAC 
ATGCTCCAAG GGGTGGCGCA GCCGAGCATG GAAGAGGGCT CTGGACAGCT CCTGGAAGGG 
CACGTGCACA TGGCTGCAGT GGACCTCCTG ATCGGTGGCA CTGAGACCAC AGCAAACACC 
CTCTCCTGGG CCGTGGTTTT TTTGCTTCAC CACCCTGAGA TTCAGCAGCG ACTGCAGGAG 
GAGCTAGACC ACGAACTGGG CCCTGGTGCC TCCAGCTCCC GGGTCCCCTA CAAGGACCGT 
GCACGGCTGC CCTTGCTCAA TGCCACCATC GCCGAGGTGC TGCGCCTGCG GCCCGTTGTG 
CCCTTAGCCT TGCCCCACCG CACCACACGG CCCAGCAGCA TCTCCGGCTA CGACATCCCT 
GAGGGCACAG TCATCATTCC GAACCTCCAA GGCGCCCACC TGGATGAGAC GGTCTGGGAG 
AGGCCACATG AGTTCTGGCC TGATCGCTTC CTGGAGCCAG GCAAGAACTC CAGAGCTCTG 
GCCTTCGGCT GCGGTGCCCG CGTGTGCCTG GGCGAGCCGC TGGCGCGCCT GGAGCTCTTC 
GTGGTGCTGA CCCGACTGCT GCAGGCCTTC ACGCTGCTGC CCTCCGGGGA CGCCCTGCCC 
TCCCTGCAGC CCCTGCCCCA CTGCAGTGTC ATCCTCAAGA TGCAGCCTTT CCAAGTGCGG 
CTGCAGCCCC GGGGGATGGG GGCCCACAGC CCGGGCCAGA GCCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024308323.1
CDS	42..1529
Translation	MLLLGLLLLLPLLAGARLLWNWWKLRSLHLPPLAPGFLHLLQPDLPIYLLGLTQKFGPIYRLHLGLQDVVVLNSKRTIEEAMVKKWADFAGRPEPLTYKLVSRNYPDLSLGDYSLLWKAHKKLTRSALLLGIRDSMEPVVEQLTQEFCERMRAQPGTPVAIEEEFSLLTCSIICYLTFGDKIKDDNLMPAYYKCIQEVLKTWSHWSIQIVDVIPFLRFFPNPGLRRLKQAIEKRDHIVEMQLRQHKESLVAGQWRDMMDYMLQGVAQPSMEEGSGQLLEGHVHMAAVDLLIGGTETTANTLSWAVVFLLHHPEIQQRLQEELDHELGPGASSSRVPYKDRARLPLLNATIAEVLRLRPVVPLALPHRTTRPSSISGYDIPEGTVIIPNLQGAHLDETVWERPHEFWPDRFLEPGKNSRALAFGCGARVCLGEPLARLELFVVLTRLLQAFTLLPSGDALPSLQPLPHCSVILKMQPFQVRLQPRGMGAHSPGQSQ

Target ORF information:

RefSeq Version	XM_024452555.1
Organism	Homo sapiens(human)
Definition	Homo sapiens cytochrome P450 family 21 subfamily A member 2 (CYP21A2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024452555.1

ORF Insert Sequence:

ATGCTGCTCC TGGGCCTGCT GCTGCTGCTG CCCCTGCTGG CTGGCGCCCG CCTGCTGTGG 
AACTGGTGGA AGCTCCGGAG CCTCCACCTC CCGCCTCTTG CCCCGGGCTT CTTGCACTTG 
CTGCAGCCCG ACCTCCCAAT CTATCTGCTT GGCCTGACTC AGAAATTCGG GCCCATCTAC 
AGGCTCCACC TTGGGCTGCA AGATGTGGTG GTGCTGAACT CCAAGAGGAC CATTGAGGAA 
GCCATGGTCA AAAAGTGGGC AGACTTTGCT GGCAGACCTG AGCCACTTAC CTACAAGCTG 
GTGTCTAGGA ACTACCCGGA CCTGTCCTTG GGAGACTACT CCCTGCTCTG GAAAGCCCAC 
AAGAAGCTCA CCCGCTCAGC CCTGCTGCTG GGCATCCGTG ACTCCATGGA GCCAGTGGTG 
GAGCAGCTGA CCCAGGAGTT CTGTGAGCGC ATGAGAGCCC AGCCCGGCAC CCCTGTGGCC 
ATTGAGGAGG AATTCTCTCT CCTCACCTGC AGCATCATCT GTTACCTCAC CTTCGGAGAC 
AAGATCAAGG ACGACAACTT AATGCCTGCC TATTACAAAT GTATCCAGGA GGTGTTAAAA 
ACCTGGAGCC ACTGGTCCAT CCAAATTGTG GACGTGATTC CCTTTCTCAG GTTCTTCCCC 
AATCCAGGTC TCCGGAGGCT GAAGCAGGCC ATAGAGAAGA GGGATCACAT CGTGGAGATG 
CAGCTGAGGC AGCACAAGGA GAGCCTCGTG GCAGGCCAGT GGAGGGACAT GATGGACTAC 
ATGCTCCAAG GGGTGGCGCA GCCGAGCATG GAAGAGGGCT CTGGACAGCT CCTGGAAGGG 
CACGTGCACA TGGCTGCAGT GGACCTCCTG ATCGGTGGCA CTGAGACCAC AGCAAACACC 
CTCTCCTGGG CCGTGGTTTT TTTGCTTCAC CACCCTGAGA TTCAGCAGCG ACTGCAGGAG 
GAGCTAGACC ACGAACTGGG CCCTGGTGCC TCCAGCTCCC GGGTCCCCTA CAAGGACCGT 
GCACGGCTGC CCTTGCTCAA TGCCACCATC GCCGAGGTGC TGCGCCTGCG GCCCGTTGTG 
CCCTTAGCCT TGCCCCACCG CACCACACGG CCCAGCAGCA TCTCCGGCTA CGACATCCCT 
GAGGGCACAG TCATCATTCC GAACCTCCAA GGCGCCCACC TGGATGAGAC GGTCTGGGAG 
AGGCCACATG AGTTCTGGCC TGATCGCTTC CTGGAGCCAG GCAAGAACTC CAGAGCTCTG 
GCCTTCGGCT GCGGTGCCCG CGTGTGCCTG GGCGAGCCGC TGGCGCGCCT GGAGCTCTTC 
GTGGTGCTGA CCCGACTGCT GCAGGCCTTC ACGCTGCTGC CCTCCGGGGA CGCCCTGCCC 
TCCCTGCAGC CCCTGCCCCA CTGCAGTGTC ATCCTCAAGA TGCAGCCTTT CCAAGTGCGG 
CTGCAGCCCC GGGGGATGGG GGCCCACAGC CCGGGCCAGA GCCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CYP21A2 ( XM_024452556.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024308324.1 Homo sapiens cytochrome P450 family 21 subfamily A member 2 (CYP21A2), transcript variant X2, mRNA.

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CloneID	OHu106317
Clone ID Related Accession (Same CDS sequence)	XM_024452556.1
Accession Version	XM_024452556.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1398bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	steroid 21-hydroxylase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_167248.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCTCC TGGGCCTGCT GCTGCTGCTG CCCCTGCTGG CTGGCGCCCG CCTGCTGTGG 
AACTGGTGGA AGCTCCGGAG CCTCCACCTC CCGCCTCTTG CCCCGGGCTT CTTGCACTTG 
CTGCAGCCCG ACCTCCCAAT CTATCTGCTT GGCCTGACTC AGAAATTCGG GCCCATCTAC 
AGGCTCCACC TTGGGCTGCA AGACAAGCTG GTGTCTAGGA ACTACCCGGA CCTGTCCTTG 
GGAGACTACT CCCTGCTCTG GAAAGCCCAC AAGAAGCTCA CCCGCTCAGC CCTGCTGCTG 
GGCATCCGTG ACTCCATGGA GCCAGTGGTG GAGCAGCTGA CCCAGGAGTT CTGTGAGCGC 
ATGAGAGCCC AGCCCGGCAC CCCTGTGGCC ATTGAGGAGG AATTCTCTCT CCTCACCTGC 
AGCATCATCT GTTACCTCAC CTTCGGAGAC AAGATCAAGG ACGACAACTT AATGCCTGCC 
TATTACAAAT GTATCCAGGA GGTGTTAAAA ACCTGGAGCC ACTGGTCCAT CCAAATTGTG 
GACGTGATTC CCTTTCTCAG GTTCTTCCCC AATCCAGGTC TCCGGAGGCT GAAGCAGGCC 
ATAGAGAAGA GGGATCACAT CGTGGAGATG CAGCTGAGGC AGCACAAGGA GAGCCTCGTG 
GCAGGCCAGT GGAGGGACAT GATGGACTAC ATGCTCCAAG GGGTGGCGCA GCCGAGCATG 
GAAGAGGGCT CTGGACAGCT CCTGGAAGGG CACGTGCACA TGGCTGCAGT GGACCTCCTG 
ATCGGTGGCA CTGAGACCAC AGCAAACACC CTCTCCTGGG CCGTGGTTTT TTTGCTTCAC 
CACCCTGAGA TTCAGCAGCG ACTGCAGGAG GAGCTAGACC ACGAACTGGG CCCTGGTGCC 
TCCAGCTCCC GGGTCCCCTA CAAGGACCGT GCACGGCTGC CCTTGCTCAA TGCCACCATC 
GCCGAGGTGC TGCGCCTGCG GCCCGTTGTG CCCTTAGCCT TGCCCCACCG CACCACACGG 
CCCAGCAGCA TCTCCGGCTA CGACATCCCT GAGGGCACAG TCATCATTCC GAACCTCCAA 
GGCGCCCACC TGGATGAGAC GGTCTGGGAG AGGCCACATG AGTTCTGGCC TGATCGCTTC 
CTGGAGCCAG GCAAGAACTC CAGAGCTCTG GCCTTCGGCT GCGGTGCCCG CGTGTGCCTG 
GGCGAGCCGC TGGCGCGCCT GGAGCTCTTC GTGGTGCTGA CCCGACTGCT GCAGGCCTTC 
ACGCTGCTGC CCTCCGGGGA CGCCCTGCCC TCCCTGCAGC CCCTGCCCCA CTGCAGTGTC 
ATCCTCAAGA TGCAGCCTTT CCAAGTGCGG CTGCAGCCCC GGGGGATGGG GGCCCACAGC 
CCGGGCCAGA GCCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024308324.1
CDS	9..1406
Translation	MLLLGLLLLLPLLAGARLLWNWWKLRSLHLPPLAPGFLHLLQPDLPIYLLGLTQKFGPIYRLHLGLQDKLVSRNYPDLSLGDYSLLWKAHKKLTRSALLLGIRDSMEPVVEQLTQEFCERMRAQPGTPVAIEEEFSLLTCSIICYLTFGDKIKDDNLMPAYYKCIQEVLKTWSHWSIQIVDVIPFLRFFPNPGLRRLKQAIEKRDHIVEMQLRQHKESLVAGQWRDMMDYMLQGVAQPSMEEGSGQLLEGHVHMAAVDLLIGGTETTANTLSWAVVFLLHHPEIQQRLQEELDHELGPGASSSRVPYKDRARLPLLNATIAEVLRLRPVVPLALPHRTTRPSSISGYDIPEGTVIIPNLQGAHLDETVWERPHEFWPDRFLEPGKNSRALAFGCGARVCLGEPLARLELFVVLTRLLQAFTLLPSGDALPSLQPLPHCSVILKMQPFQVRLQPRGMGAHSPGQSQ

Target ORF information:

RefSeq Version	XM_024452556.1
Organism	Homo sapiens(human)
Definition	Homo sapiens cytochrome P450 family 21 subfamily A member 2 (CYP21A2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024452556.1

ORF Insert Sequence:

ATGCTGCTCC TGGGCCTGCT GCTGCTGCTG CCCCTGCTGG CTGGCGCCCG CCTGCTGTGG 
AACTGGTGGA AGCTCCGGAG CCTCCACCTC CCGCCTCTTG CCCCGGGCTT CTTGCACTTG 
CTGCAGCCCG ACCTCCCAAT CTATCTGCTT GGCCTGACTC AGAAATTCGG GCCCATCTAC 
AGGCTCCACC TTGGGCTGCA AGACAAGCTG GTGTCTAGGA ACTACCCGGA CCTGTCCTTG 
GGAGACTACT CCCTGCTCTG GAAAGCCCAC AAGAAGCTCA CCCGCTCAGC CCTGCTGCTG 
GGCATCCGTG ACTCCATGGA GCCAGTGGTG GAGCAGCTGA CCCAGGAGTT CTGTGAGCGC 
ATGAGAGCCC AGCCCGGCAC CCCTGTGGCC ATTGAGGAGG AATTCTCTCT CCTCACCTGC 
AGCATCATCT GTTACCTCAC CTTCGGAGAC AAGATCAAGG ACGACAACTT AATGCCTGCC 
TATTACAAAT GTATCCAGGA GGTGTTAAAA ACCTGGAGCC ACTGGTCCAT CCAAATTGTG 
GACGTGATTC CCTTTCTCAG GTTCTTCCCC AATCCAGGTC TCCGGAGGCT GAAGCAGGCC 
ATAGAGAAGA GGGATCACAT CGTGGAGATG CAGCTGAGGC AGCACAAGGA GAGCCTCGTG 
GCAGGCCAGT GGAGGGACAT GATGGACTAC ATGCTCCAAG GGGTGGCGCA GCCGAGCATG 
GAAGAGGGCT CTGGACAGCT CCTGGAAGGG CACGTGCACA TGGCTGCAGT GGACCTCCTG 
ATCGGTGGCA CTGAGACCAC AGCAAACACC CTCTCCTGGG CCGTGGTTTT TTTGCTTCAC 
CACCCTGAGA TTCAGCAGCG ACTGCAGGAG GAGCTAGACC ACGAACTGGG CCCTGGTGCC 
TCCAGCTCCC GGGTCCCCTA CAAGGACCGT GCACGGCTGC CCTTGCTCAA TGCCACCATC 
GCCGAGGTGC TGCGCCTGCG GCCCGTTGTG CCCTTAGCCT TGCCCCACCG CACCACACGG 
CCCAGCAGCA TCTCCGGCTA CGACATCCCT GAGGGCACAG TCATCATTCC GAACCTCCAA 
GGCGCCCACC TGGATGAGAC GGTCTGGGAG AGGCCACATG AGTTCTGGCC TGATCGCTTC 
CTGGAGCCAG GCAAGAACTC CAGAGCTCTG GCCTTCGGCT GCGGTGCCCG CGTGTGCCTG 
GGCGAGCCGC TGGCGCGCCT GGAGCTCTTC GTGGTGCTGA CCCGACTGCT GCAGGCCTTC 
ACGCTGCTGC CCTCCGGGGA CGCCCTGCCC TCCCTGCAGC CCCTGCCCCA CTGCAGTGTC 
ATCCTCAAGA TGCAGCCTTT CCAAGTGCGG CTGCAGCCCC GGGGGATGGG GGCCCACAGC 
CCGGGCCAGA GCCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sensitive Monogenic Noninvasive Prenatal Diagnosis by Targeted Haplotyping.
American journal of human genetics101(3)326-339(2017 Sep)
Vermeulen C,Geeven G,de Wit E,Verstegen MJAM,Jansen RPM,van Kranenburg M,de Bruijn E,Pulit SL,Kruisselbrink E,Shahsavari Z,Omrani D,Zeinali F,Najmabadi H,Katsila T,Vrettou C,Patrinos GP,Traeger-Synodinos J,Splinter E,Beekman JM,Kheradmand Kia S,Te Meerman GJ,Ploos van Amstel HK,de Laat W

Functional analysis of human cytochrome P450 21A2 variants involved in congenital adrenal hyperplasia.
The Journal of biological chemistry292(26)10767-10778(2017 06)
Wang C,Pallan PS,Zhang W,Lei L,Yoshimoto FK,Waterman MR,Egli M,Guengerich FP

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CYP21A2 cDNA ORF clone, Homo sapiens(human)

Gene

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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