FREM1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	FREM1
Entrez Gene ID	158326
Full Name	FRAS1 related extracellular matrix 1
Synonyms	BNAR,C9orf143,C9orf145,C9orf154,MOTA,TILRR,TRIGNO2
General protein information	Preferred Names FRAS1 related extracellular matrix 1 Names FRAS1-related extracellular matrix protein 1 extracellular matrix protein QBRICK
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 9 Map Location: 9p22.3
Summary	This gene encodes a basement membrane protein that may play a role in craniofacial and renal development. Mutations in this gene have been associated with bifid nose with or without anorectal and renal anomalies. Alternatively spliced transcript variants encoding different isoforms have been described. PubMed ID 19940113 describes one such variant that initiates transcription within a distinct, internal exon; the resulting shorter isoform (named Toll-like/interleukin-1 receptor regulator, TILRR) is suggested to be a co-receptor of the interleukin 1 receptor family and may regulate receptor function and Toll-like receptor/interleukin 1 receptor signal transduction, contributing to the control of inflammatory response activation. [provided by RefSeq, Apr 2011].
Disorder MIM:	608944
Disorder Html:	Bifid nose with or without anorectal and renal anomalies, 608980 (3); Manitoba oculotrichoanal syndrome, 248450 (3); Trigonocephaly 2, 614485 (3)

mRNA and Protein(s)

mRNA	Protein	Name
XM_017014320.1	XP_016869809.1	FRAS1-related extracellular matrix protein 1 isoform X1
XM_017014328.1	XP_016869817.1	FRAS1-related extracellular matrix protein 1 isoform X7
XM_017014325.1	XP_016869814.1	FRAS1-related extracellular matrix protein 1 isoform X4
NM_001177704.1	NP_001171175.1	FRAS1-related extracellular matrix protein 1 isoform 2
NM_001370065.1	NP_001356994.1	FRAS1-related extracellular matrix protein 1 isoform 5 precursor
XM_005251382.4	XP_005251439.1	FRAS1-related extracellular matrix protein 1 isoform X2
XM_017014325.2	XP_016869814.1	FRAS1-related extracellular matrix protein 1 isoform X4
XM_017014326.1	XP_016869815.1	FRAS1-related extracellular matrix protein 1 isoform X5
XM_017014318.1	XP_016869807.1	FRAS1-related extracellular matrix protein 1 isoform X1
XM_017014316.1	XP_016869805.1	FRAS1-related extracellular matrix protein 1 isoform X1
XM_017014327.1	XP_016869816.1	FRAS1-related extracellular matrix protein 1 isoform X6
XM_017014324.1	XP_016869813.1	FRAS1-related extracellular matrix protein 1 isoform X3
XM_005251382.3	XP_005251439.1	FRAS1-related extracellular matrix protein 1 isoform X2
NM_001370061.1	NP_001356990.1	FRAS1-related extracellular matrix protein 1 isoform 2
XM_017014316.2	XP_016869805.1	FRAS1-related extracellular matrix protein 1 isoform X1
XM_017014317.1	XP_016869806.1	FRAS1-related extracellular matrix protein 1 isoform X1
XM_017014319.2	XP_016869808.1	FRAS1-related extracellular matrix protein 1 isoform X1
XM_017014321.2	XP_016869810.1	FRAS1-related extracellular matrix protein 1 isoform X1
XM_017014323.1	XP_016869812.1	FRAS1-related extracellular matrix protein 1 isoform X1
XM_017014327.2	XP_016869816.1	FRAS1-related extracellular matrix protein 1 isoform X6
XM_017014330.2	XP_016869819.1	FRAS1-related extracellular matrix protein 1 isoform X9
XM_017014329.1	XP_016869818.1	FRAS1-related extracellular matrix protein 1 isoform X8
NM_001370058.1	NP_001356987.1	FRAS1-related extracellular matrix protein 1 isoform 3
NM_001370063.1	NP_001356992.1	FRAS1-related extracellular matrix protein 1 isoform 5 precursor
XM_017014322.1	XP_016869811.1	FRAS1-related extracellular matrix protein 1 isoform X1
XM_017014324.2	XP_016869813.1	FRAS1-related extracellular matrix protein 1 isoform X3
XM_017014328.2	XP_016869817.1	FRAS1-related extracellular matrix protein 1 isoform X7
XM_017014321.1	XP_016869810.1	FRAS1-related extracellular matrix protein 1 isoform X1
XM_017014320.2	XP_016869809.1	FRAS1-related extracellular matrix protein 1 isoform X1
XM_017014329.2	XP_016869818.1	FRAS1-related extracellular matrix protein 1 isoform X8
XM_011517758.2	XP_011516060.1	FRAS1-related extracellular matrix protein 1 isoform X10
XM_006716729.3	XP_006716792.1	FRAS1-related extracellular matrix protein 1 isoform X11
XM_017014319.1	XP_016869808.1	FRAS1-related extracellular matrix protein 1 isoform X1
XM_017014330.1	XP_016869819.1	FRAS1-related extracellular matrix protein 1 isoform X9
NM_001370060.1	NP_001356989.1	FRAS1-related extracellular matrix protein 1 isoform 4 precursor
XM_005251384.4	XP_005251441.1	FRAS1-related extracellular matrix protein 1 isoform X11
NM_144966.5	NP_659403.4	FRAS1-related extracellular matrix protein 1 isoform 1 precursor

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	FREM1	XP_520492.3
Canis lupus familiaris (dog)	FREM1	XP_005626693.1
Homo sapiens (human)	FREM1	NP_659403.4
	FREM1	XP_001110764.1
Rattus norvegicus (Norway rat)	Frem1	NP_001178686.1
Bos taurus (cattle)	FREM1	NP_001179924.1
Danio rerio (zebrafish)	frem1a	NP_001177237.1
Mus musculus (house mouse)	Frem1	NP_001185740.1
Gallus gallus (chicken)	FREM1	XP_424932.2
Xenopus tropicalis (tropical clawed frog)	LOC100488523	XP_002939136.2

Gene Ontology
Bibliography

Related articles in PubMed

Human gingiva transcriptome during wound healing.
Wang Y, Tatakis DN
Journal of clinical periodontology44(4)394-402(2017 Apr)

Novel FREM1 mutations in a patient with MOTA syndrome: Clinical findings, mutation update and review of FREM1-related disorders literature.
Chacon-Camacho OF, Zenker M, Schanze D, Ledesma-Gil J, Zenteno JC
European journal of medical genetics60(3)190-194(2017 Mar)

Genome-wide association study of facial morphology reveals novel associations with FREM1 and PARK2.
Lee MK, Shaffer JR, Leslie EJ, Orlova E, Carlson JC, Feingold E, Marazita ML, Weinberg SM
PloS one12(4)e0176566(2017)

Mild recessive mutations in six Fraser syndrome-related genes cause isolated congenital anomalies of the kidney and urinary tract.
Kohl S, Hwang DY, Dworschak GC, Hilger AC, Saisawat P, Vivante A, Stajic N, Bogdanovic R, Reutter HM, Kehinde EO, Tasic V, Hildebrandt F
Journal of the American Society of Nephrology : JASN25(9)1917-22(2014 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

FREM1 expression is significantly downregulated in human masticatory mucosa during wound healing
Title: Human gingiva transcriptome during wound healing.

this report describes a patient with a phenotype compatible with Manitoba-oculo-tricho-anal syndrome in whom two novel FREM1 mutations were identified in the compound heterozygous state thus broadening the mutational spectrum of the disease.
Title: Novel FREM1 mutations in a patient with MOTA syndrome: Clinical findings, mutation update and review of FREM1-related disorders literature.

we identified genome-wide significant association involving measures of central upper lip height at 9p22 within FREM1
Title: Genome-wide association study of facial morphology reveals novel associations with FREM1 and PARK2.

In 15 of 590 families, we identified recessive mutations in the genes FRAS1, FREM2, GRIP1, FREM1, ITGA8, and GREM1, all of which function in the interaction of the ureteric bud and the metanephric mesenchyme.
Title: Mild recessive mutations in six Fraser syndrome-related genes cause isolated congenital anomalies of the kidney and urinary tract.

Disruption of the FREM1 gene can produce a spectrum of clinical manifestations encompassing the previously described MOTA and BNAR syndromes.
Title: Novel FREM1 mutations expand the phenotypic spectrum associated with Manitoba-oculo-tricho-anal (MOTA) syndrome and bifid nose renal agenesis anorectal malformations (BNAR) syndrome.

The following FREM1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the FREM1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu22151	NM_144966.5 Latest version!	Homo sapiens FRAS1 related extracellular matrix 1 (FREM1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$779.50-$1091.30 $1559.00
OHu22151	XM_005251382.4 Latest version!	Homo sapiens FRAS1 related extracellular matrix 1 (FREM1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$779.50-$1091.30 $1559.00
OHu108040	NM_001370060.1 Latest version!	Homo sapiens FRAS1 related extracellular matrix 1 (FREM1), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OHu108042	NM_001370065.1 Latest version!	Homo sapiens FRAS1 related extracellular matrix 1 (FREM1), transcript variant 7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OHu108042	NM_001370063.1 Latest version!	Homo sapiens FRAS1 related extracellular matrix 1 (FREM1), transcript variant 6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OHu22950	NM_001370061.1 Latest version!	Homo sapiens FRAS1 related extracellular matrix 1 (FREM1), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OHu22950	NM_001177704.1 Latest version!	Homo sapiens FRAS1 related extracellular matrix 1 (FREM1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OHu22950	XM_006716729.3 Latest version!	Homo sapiens FRAS1 related extracellular matrix 1 (FREM1), transcript variant X21, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OHu22950	XM_005251384.4 Latest version!	Homo sapiens FRAS1 related extracellular matrix 1 (FREM1), transcript variant X22, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OHu76988	XM_011517758.2 Latest version!	Homo sapiens FRAS1 related extracellular matrix 1 (FREM1), transcript variant X20, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OHu108041	NM_001370058.1 Latest version!	Homo sapiens FRAS1 related extracellular matrix 1 (FREM1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OHu98466	XM_017014330.2 Latest version!	Homo sapiens FRAS1 related extracellular matrix 1 (FREM1), transcript variant X19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OHu98463	XM_017014327.2 Latest version!	Homo sapiens FRAS1 related extracellular matrix 1 (FREM1), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu98464	XM_017014328.2 Latest version!	Homo sapiens FRAS1 related extracellular matrix 1 (FREM1), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu98465	XM_017014329.2 Latest version!	Homo sapiens FRAS1 related extracellular matrix 1 (FREM1), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu98459	XM_017014316.2 Latest version!	Homo sapiens FRAS1 related extracellular matrix 1 (FREM1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OHu98459	XM_017014321.2 Latest version!	Homo sapiens FRAS1 related extracellular matrix 1 (FREM1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OHu98459	XM_017014320.2 Latest version!	Homo sapiens FRAS1 related extracellular matrix 1 (FREM1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OHu98459	XM_017014317.1 Latest version!	Homo sapiens FRAS1 related extracellular matrix 1 (FREM1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OHu98459	XM_017014323.1 Latest version!	Homo sapiens FRAS1 related extracellular matrix 1 (FREM1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OHu98459	XM_017014318.1 Latest version!	Homo sapiens FRAS1 related extracellular matrix 1 (FREM1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OHu98459	XM_017014319.2 Latest version!	Homo sapiens FRAS1 related extracellular matrix 1 (FREM1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OHu98459	XM_017014322.1 Latest version!	Homo sapiens FRAS1 related extracellular matrix 1 (FREM1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OHu98460	XM_017014324.2 Latest version!	Homo sapiens FRAS1 related extracellular matrix 1 (FREM1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OHu98461	XM_017014325.2 Latest version!	Homo sapiens FRAS1 related extracellular matrix 1 (FREM1), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OHu98462	XM_017014326.1 Latest version!	Homo sapiens FRAS1 related extracellular matrix 1 (FREM1), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
View Old Accession Versions >>
OHu98461	XM_017014325.1	Homo sapiens FRAS1 related extracellular matrix 1 (FREM1), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OHu22151	XM_005251382.3	Homo sapiens FRAS1 related extracellular matrix 1 (FREM1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$779.50-$1091.30 $1559.00
OHu98459	XM_017014316.1	Homo sapiens FRAS1 related extracellular matrix 1 (FREM1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OHu98463	XM_017014327.1	Homo sapiens FRAS1 related extracellular matrix 1 (FREM1), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu98460	XM_017014324.1	Homo sapiens FRAS1 related extracellular matrix 1 (FREM1), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OHu98464	XM_017014328.1	Homo sapiens FRAS1 related extracellular matrix 1 (FREM1), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu98459	XM_017014321.1	Homo sapiens FRAS1 related extracellular matrix 1 (FREM1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OHu98459	XM_017014320.1	Homo sapiens FRAS1 related extracellular matrix 1 (FREM1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OHu98465	XM_017014329.1	Homo sapiens FRAS1 related extracellular matrix 1 (FREM1), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu98459	XM_017014319.1	Homo sapiens FRAS1 related extracellular matrix 1 (FREM1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OHu98466	XM_017014330.1	Homo sapiens FRAS1 related extracellular matrix 1 (FREM1), transcript variant X20, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

FREM1 ( NM_144966.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_659403.4 Homo sapiens FRAS1 related extracellular matrix 1 (FREM1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu22151
Clone ID Related Accession (Same CDS sequence)	XM_005251382.4 , XM_005251382.3 , NM_144966.5
Accession Version	NM_144966.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6540bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Homo sapiens(human)
Product	FRAS1-related extracellular matrix protein 1 isoform 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL354672.27, BX537574.1, DN992860.1, AB160987.1, AL512643.9 and AL390732.10. This sequence is a reference standard in the RefSeqGene project. On Apr 27, 2010 this sequence version replaced NM_144966.4. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: AB160987.1 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGAACTCTC TGAGTTGGGG GGCTGCGAAT GCCGTGCTGC TGCTGCTCCT CCTGGCCTGG 
GCCAGCCCCA CCTTCATCAG CATCAACCGC GGGGTGAGGG TGATGAAGGG CCACTCTGCC 
TTCCTGTCAG GAGATGACCT GAAGTTTGCC ATCCCTAAAG AGAAAGATGC CTGCAAAGTG 
GAAGTTGTGA TGAATGAGCC AATAACCCAG AGGGTTGGGA AACTCACTCC ACAGGTCTTT 
GACTGCCATT TCCTTCCCAA CGAAGTCAAG TATGTTCACA ATGGTTGTCC AATTCTTGAT 
GAAGACACAG TGAAGCTCAG ACTTTACAGA TTTACTGAAA GAGATACCTT CATAGAAACT 
TTTATCCTGT GGGTCTATCT CCTGGAACCA GACTGTAACA TCATCCATAT GAGTAACAAT 
GTGCTGGAGG TGCCTGAATT CAATGGCTTG TCCCAAGCGA TTGATAAAAA TCTGCTCAGA 
TTCGATTATG ATAGGATGGC TAGCCTGGAA TGTACCGTCA GCCTGGACAC TGCGAGAACT 
CGGCTGCCAG CCCATGGCCA GATGGTTCTC GGGGAGCCTC GACCAGAAGA ACCTCGTGGA 
GATCAGCCAC ACAGTTTTTT CCCAGAGTCC CAACTGAGAG CAAAACTGAA GTGTCCAGGT 
GGGAGCTGTA CCCCAGGATT AAAGAAAATA GGAAGCCTCA AAGTGAGCTG TGAGGAGTTC 
CTGCTGATGG GCCTTCGTTA TCAGCATCTG GATCCCCCTT CACCCAACAT TGATTATATC 
TCCATCCAAC TGGACCTCAC AGATACCAGG AGCAAAATTG TGTACAAGTC AGAGAGTGCG 
TGGCTGCCTG TCTATATCAG AGCTGGAATT CCGAATCAGA TTCCAAAGGC TGCATTCATG 
GCCGTGTTTA TTCTGGAAGT GGATCAGTTC ATCCTGACCT CCTTGACTAC ATCAGTTCTG 
GACTGTGAAG AAGATGAGAC CCCTAAACCC TTGCTGGTGT TCAACATTAC TAAAGCCCCG 
CTCCAGGGCT ATGTGACTCA CCTGTTGGAT CACACCAGAC CAATCTCCTC ATTCACCTGG 
AAAGATCTCA GTGACATGCA GATCGCCTAT CAGCCACCAA ACAGCAGCCA TTCTGAGAGG 
AGACATGATG AGGTAGAATT GGAGGTATAC GACTTCTTCT TTGAAAGGAG TGCACCTATG 
ACAGTCCACA TCTCCATCAG AACAGCAGAT ACAAATGCCC CCCGTGTATC CTGGAATACA 
GGTCTGAGTC TCCTTGAGGG GCAGTCTCGA GCCATCACTT GGGAACAGTT TCAGGTTGTC 
GACAATGACG ACATTGGTGC TGTCCGGCTA GTCACCGTTG GTGGCCTGCA GCATGGATGG 
CTGACTTTAA GAGGGGGGAA AGGGTTTCTC TTCACCGTGG CTGACCTCCA GGCTGGAGTT 
GTTCGCTATC ATCATGATGA CAGCGACTCC ACCAAAGACT TCGTGGTCTT CCGGATATTT 
GATGGCCATC ACAGCATCCG TCACAAATTC CCCATCAACG TCTTGCCCAA AGATGATAGT 
CCCCCGTTCC TCATAACCAA TGTTGTGATT GAACTGGAGG AGGGGCAGAC CATCCTGATC 
CAGGGATCCA TGCTGCGAGC TTCAGATGTG GACGCCAGTG ATGACTACAT CTTCTTCAAT 
ATCACAAAGC CTCCACAGGC TGGGGAGATC ATGAAGAAGC CAGGGCCAGG ACTGATAGGC 
TATCCTGTCC ATGGCTTCCT TCAGAGGGAT TTGTTTAATG GAATCATTTA TTATCGTCAT 
TTTGGTGGAG AAATCTTTGA AGATTCTTTT CAATTTGTCC TGTGGGACAG CCATGAACCT 
CCAAATCTTT CAGTGCCACA GGTGGCAACA ATCCATATAA CTCCAGTGGA TGACCAGCTT 
CCAAAAGAGG CTCCTGGAGT TTCTCGGCAT TTGGTTGTCA AGGAAACTGA GGTGGCCTAT 
ATAACTAAGA AACAGCTACA TTTTATAGAT TCAGAATCAT ATGACAGGGA GCTGGTCTAC 
ACAATAACTA CTCCTCCATT TTTCTCCTTC AGCCACAGAC ACTTGGATGC TGGGAAATTA 
TTTATGGTGG ACAGCATACC AAAAGTAGTT AAGAATCCTA CGGCCCTGGA GCTGAGGTCA 
TTCACTCAGC ATGCTGTGAA CTATATGAAA GTGGCCTACA TGCCCCCCAT GCAAGACATT 
GGTCCCCATT GCAGAGATGT CCAGTTCACA TTTTCTGTCA GTAACCAACA TGGCGGTACT 
TTGCATGGGA TCTGCTTTAA CATTACAATC CTCCCAGTGG ACAATCAAGT TCCTGAGGCG 
TTCACCAACC CTCTGAAAGT GACTGAGGGA GGTCAAAGCA TCATCAGCAC AGAGCACATT 
CTAATTTCTG ATGCAGATAC CAAGCTGGAC AATATTGACC TCTCCCTGCG GGAATTGCCT 
CTGCACGGAA GGGTGGAGCT GAATGGATTT CCTCTAAATT CAGGGGGCAC ATTTTCTTGG 
GGCGATCTCC ATACCTTAAA AGTTAGGTAT CAACATGATG GAACTGAAGT TCTTCAGGAT 
GACCTACTCT TGGAGGTCAC CGATGGCACA AATTCAGCAG AATTTGTACT ACATGTTGAG 
GTATTCCCTG TCAACGATGA GCCACCAGTC TTAAAGGCTG ACCTCATGCC TGTCATGAAT 
TGCTCAGAGG GAGGAGAGGT GGTCATCACC TCTGAATACA TTTTTGCTAC TGATGTGGAC 
AGCGATAACT TGAAGTTGAT GTTTGTGATT GCTCGCGAAC CTCAGCATGG GGTGGTGAGG 
AGAGCTGGAG TCACAGTGGA TCAGTTCTCT CAGAGAGATG TTATCTCAGA GGCCGTGACA 
TACAAACACA CAGGTGGCGA GATTGGCCTG ATGCCTTGTT TTGACACCAT TACATTGGTG 
GTTTCGGATG GAGAGGCTGG CCCTTTTGTG AATGGCTGTT GCTACAATGG ACCCAATCCA 
TCTGTTCCAC TTCATGCGTC CTTTCCAGTG TATGATCTCA ACATCACGGT ATACCCAGTA 
GACAACCAGC CACCTTCCAT TGCAATAGGT CCCGTGTTTG TTGTAGATGA GGGCTGCTCA 
ACAGCCCTTA CTGTTAACCA TTTGTCCGCC ACTGACCCTG ACACTGCAGC AGATGACTTG 
GAATTTGTTT TGGTTTCTCC TCCTCAGTTT GGCTACCTCG AAAATATACT CCCTTCTGTG 
GGTTTTGAAA AAAGCAATAT TGGCATAAGT ATAGATTCAT TTCAGTGGAA AGACATGAAC 
GCTTTTCACA TTAACTATGT GCAGTCCAGG CATCTGAGGA TAGAACCAAC TGCCGACCAG 
TTCACGGTGT ACGTCACAGA TGGGAAGCAT CACTCCTTGG AGATACCATT TTCTATTATA 
ATCAACCCCA CAAATGATGA AGCTCCTGAC TTTGTAGTGC AGAATATTAC TGTGTGTGAG 
GGTCAGATGA AAGAGCTGGA CTCTTCCATC ATCAGCGCTG TGGACCTGGA CATTCCCCAG 
GATGCCCTGC TGTTCAGCAT CACTCAAAAG CCACGCCATG GCCTCCTCAT CGATAGGGGG 
TTTAGCAAAG ACTTCTCTGA GAATAAGCAG CCAGCCAACC CTCACCAGAA ACATGCACCT 
GTTCACAGCT TTTCCATGGA ACTCCTCAAG ACTGGAATGA GGTTGACGTA CATGCATGAT 
GACTCAGAGA GCCTTGCTGA TGATTTTACA ATCCAATTGT CAGATGGGAA ACATAAGATA 
CTTAAAACCA TTTCAGTAGA GGTCATCCCA GTTAATGATG AAAAACCAAT GCTGAGCAAG 
AAGGCTGAAA TTGCAATGAA TATGGGTGAA ACTCGTATTA TTTCCAGTGC TATTCTTTCA 
GCCATAGATG AAGACTCACC CAGGGAGAAG ATTTACTATG TATTTGAAAG GCTTCCCCAA 
AATGGGCAAC TTCAGCTTAA GATAGGGAGG GACTGGGTTC CTCTCTCCCC TGGCATGAAA 
TGCACTCAGG AGGAAGTGGA TCTGAACTTG CTGAGATACA CACACACCGG GGCAATGGAT 
TCCCAGAATC AAGATAGCTT CACCTTCTAC CTTTGGGATG GCAACAACAG GTCCCCTGCT 
CTTGACTGTC AAATCACCAT CAAGGACATG GAAAAAGGTG ATATTGTCAT CCTTACAAAA 
CCACTCGTGG TGTCTAAAGG TGATAGAGGT TTCTTAACAA CCACCACGCT CCTGGCTGTG 
GACGGAACAG ACAAGCCTGA GGAACTGCTC TATGTCATCA CCTCCCCTCC GCGATATGGC 
CAGATCGAAT ATGTTCACTA TCCTGGAGTT CCCATTACAA ACTTCAGCCA AATGGATGTA 
GTGGGGCAGA CAGTTTGCTA TGTACACAAG AGCAAGGTGA CTGTCTCCAG TGACAGATTC 
AGATTCATCA TCAGCAATGG ACTGCGGACC GAGCACGGGG TGTTTGAGAT CACACTGGAG 
ACTGTGGACA GAGCCCTGCC TGTGGTAACC AGGAACAAGG GGTTGAGACT GGCCCAAGGG 
GCCGTGGGCC TGCTTTCCCC TGACCTCCTT CAGCTGACCG ACCCTGATAC ACCTGCGGAG 
AACCTCACCT TCCTCTTGGT TCAGCTCCCC CAGCATGGCC AGCTCTACCT GTGGGGGACA 
GGGCTACTTC AACACAATTT CACCCAGCAG GATGTGGACA GCAAGAATGT GGCCTATCGG 
CACTCAGGAG GGGACTCCCA GACTGACTGC TTTACTTTCA TGGCCACAGA TGGGACAAAC 
CAAGGCTTTA TTGTGAATGG GAGAGTGTGG GAAGAACCTG TTTTATTCAC CATTCAGGTA 
GACCAATTGG ACAAAACAGC TCCTCGTATC ACACTCTTGC ATTCCCCTTC TCAAGTGGGG 
CTCCTGAAAA ATGGCTGCTA CGGGATTTAC ATCACTTCCC GCGTGTTGAA GGCATCAGAC 
CCTGACACTG AGGACGATCA GATCATCTTT AAAATTCTAC AAGGCCCAAA ACATGGACAT 
CTGGAGAACA CAACAACAGG TGAATTTATC CATGAGAAAT TTAGCCAAAA GGACTTAAAC 
AGTAAGACTA TTCTTTACAT CATAAACCCA TCTTTGGAAG TAAATTCAGA TACCGTGGAA 
TTTCAAATCA TGGACCCCAC AGGGAACTCG GCCACTCCTC AAATTTTGGA ACTGAAGTGG 
TCTCATATTG AATGGTCACA GACCGAATAT GAAGTCTGTG AGAATGTGGG TTTGTTGCCC 
TTGGAAATTA TCAGAAGGGG ATATTCCATG GACTCGGCCT TTGTGGGTAT AAAGGTCAAC 
CAAGTGTCAG CTGCAGTTGG AAAAGATTTC ACCGTGATTC CATCTAAACT GATTCAGTTT 
GACCCAGGAA TGTCAACTAA GATGTGGAAT ATAGCAATTA CCTATGACGG ATTAGAGGAA 
GATGATGAGG TCTTTGAAGT AATTCTGAAC TCCCCTGTGA ATGCAGTTCT TGGCACAAAG 
ACAAAAGCTG CAGTGAAAAT TTTGGACTCA AAAGGAGGAC AATGCCATCC TTCATATTCC 
TCCAACCAAA GCAAGCACAG CACATGGGAG AAGGGCATTT GGCATCTGCT GCCCCCAGGG 
TCTTCCTCAT CCACCACTTC TGGTTCCTTT CATCTGGAAA GAAGACCTCT TCCATCTTCC 
ATGCAGCTAG CAGTCATCAG GGGAGACACC CTGCGGGGCT TTGATTCTAC AGATCTTTCT 
CAAAGGAAGC TTAGGACCCG TGGGAATGGC AAAACAGTTC GTCCATCCTC TGTTTATAGA 
AATGGAACAG ACATCATCTA TAATTATCAT GGGATAGTTT CCTTGAAACT GGAGGATGAC 
AGTTTCCCAA CTCACAAAAG GAAGGCCAAA GTATCCATCA TTAGTCAGCC ACAAAAGACA 
ATCAAAGTGG CAGAACTGCC TCAAGCAGAT AAGGTGGAAT CCACAACTGA CTCACACTTC 
CCCAGACAGG ACCAGTTGCC CTCATTTCCA AAGAACTGCA CTCTGGAATT AAAGGGACTC 
TTCCATTTTG AAGAAGGCAT CCAGAAGCTG TATCAGTGCA ATGGGATCGC CTGGAAAGCC 
TGGAGTCCCC AAACCAAGGA TGTGGAAGAC AAATCCTGTC CAGCCGGGTG GCACCAGCAC 
TCAGGCTACT GTCACATCTT GATCACAGAG CAGAAAGGCA CCTGGAATGC GGCTGCCCAA 
GCTTGCAGGG AACAATACCT GGGCAACCTT GTAACTGTAT TCTCCAGGCA GCACATGCGG 
TGGCTCTGGG ACATTGGTGG GAGAAAGTCC TTTTGGATAG GTTTGAACGA CCAAGTGCAT 
GCTGGCCACT GGGAGTGGAT CGGTGGTGAA CCTGTTGCCT TCACCAATGG GAGAAGAGGG 
CCCTCTCAAC GCTCCAAGCT TGGAAAGAGC TGTGTTTTGG TTCAAAGACA AGGGAAATGG 
CAAACAAAAG ACTGTAGGAG AGCCAAACCT CATAATTATG TGTGTTCCAG AAAACTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_659403.4
CDS	817..7356
Translation	MNSLSWGAANAVLLLLLLAWASPTFISINRGVRVMKGHSAFLSGDDLKFAIPKEKDACKVEVVMNEPITQRVGKLTPQVFDCHFLPNEVKYVHNGCPILDEDTVKLRLYRFTERDTFIETFILWVYLLEPDCNIIHMSNNVLEVPEFNGLSQAIDKNLLRFDYDRMASLECTVSLDTARTRLPAHGQMVLGEPRPEEPRGDQPHSFFPESQLRAKLKCPGGSCTPGLKKIGSLKVSCEEFLLMGLRYQHLDPPSPNIDYISIQLDLTDTRSKIVYKSESAWLPVYIRAGIPNQIPKAAFMAVFILEVDQFILTSLTTSVLDCEEDETPKPLLVFNITKAPLQGYVTHLLDHTRPISSFTWKDLSDMQIAYQPPNSSHSERRHDEVELEVYDFFFERSAPMTVHISIRTADTNAPRVSWNTGLSLLEGQSRAITWEQFQVVDNDDIGAVRLVTVGGLQHGWLTLRGGKGFLFTVADLQAGVVRYHHDDSDSTKDFVVFRIFDGHHSIRHKFPINVLPKDDSPPFLITNVVIELEEGQTILIQGSMLRASDVDASDDYIFFNITKPPQAGEIMKKPGPGLIGYPVHGFLQRDLFNGIIYYRHFGGEIFEDSFQFVLWDSHEPPNLSVPQVATIHITPVDDQLPKEAPGVSRHLVVKETEVAYITKKQLHFIDSESYDRELVYTITTPPFFSFSHRHLDAGKLFMVDSIPKVVKNPTALELRSFTQHAVNYMKVAYMPPMQDIGPHCRDVQFTFSVSNQHGGTLHGICFNITILPVDNQVPEAFTNPLKVTEGGQSIISTEHILISDADTKLDNIDLSLRELPLHGRVELNGFPLNSGGTFSWGDLHTLKVRYQHDGTEVLQDDLLLEVTDGTNSAEFVLHVEVFPVNDEPPVLKADLMPVMNCSEGGEVVITSEYIFATDVDSDNLKLMFVIAREPQHGVVRRAGVTVDQFSQRDVISEAVTYKHTGGEIGLMPCFDTITLVVSDGEAGPFVNGCCYNGPNPSVPLHASFPVYDLNITVYPVDNQPPSIAIGPVFVVDEGCSTALTVNHLSATDPDTAADDLEFVLVSPPQFGYLENILPSVGFEKSNIGISIDSFQWKDMNAFHINYVQSRHLRIEPTADQFTVYVTDGKHHSLEIPFSIIINPTNDEAPDFVVQNITVCEGQMKELDSSIISAVDLDIPQDALLFSITQKPRHGLLIDRGFSKDFSENKQPANPHQKHAPVHSFSMELLKTGMRLTYMHDDSESLADDFTIQLSDGKHKILKTISVEVIPVNDEKPMLSKKAEIAMNMGETRIISSAILSAIDEDSPREKIYYVFERLPQNGQLQLKIGRDWVPLSPGMKCTQEEVDLNLLRYTHTGAMDSQNQDSFTFYLWDGNNRSPALDCQITIKDMEKGDIVILTKPLVVSKGDRGFLTTTTLLAVDGTDKPEELLYVITSPPRYGQIEYVHYPGVPITNFSQMDVVGQTVCYVHKSKVTVSSDRFRFIISNGLRTEHGVFEITLETVDRALPVVTRNKGLRLAQGAVGLLSPDLLQLTDPDTPAENLTFLLVQLPQHGQLYLWGTGLLQHNFTQQDVDSKNVAYRHSGGDSQTDCFTFMATDGTNQGFIVNGRVWEEPVLFTIQVDQLDKTAPRITLLHSPSQVGLLKNGCYGIYITSRVLKASDPDTEDDQIIFKILQGPKHGHLENTTTGEFIHEKFSQKDLNSKTILYIINPSLEVNSDTVEFQIMDPTGNSATPQILELKWSHIEWSQTEYEVCENVGLLPLEIIRRGYSMDSAFVGIKVNQVSAAVGKDFTVIPSKLIQFDPGMSTKMWNIAITYDGLEEDDEVFEVILNSPVNAVLGTKTKAAVKILDSKGGQCHPSYSSNQSKHSTWEKGIWHLLPPGSSSSTTSGSFHLERRPLPSSMQLAVIRGDTLRGFDSTDLSQRKLRTRGNGKTVRPSSVYRNGTDIIYNYHGIVSLKLEDDSFPTHKRKAKVSIISQPQKTIKVAELPQADKVESTTDSHFPRQDQLPSFPKNCTLELKGLFHFEEGIQKLYQCNGIAWKAWSPQTKDVEDKSCPAGWHQHSGYCHILITEQKGTWNAAAQACREQYLGNLVTVFSRQHMRWLWDIGGRKSFWIGLNDQVHAGHWEWIGGEPVAFTNGRRGPSQRSKLGKSCVLVQRQGKWQTKDCRRAKPHNYVCSRKL

Target ORF information:

RefSeq Version	NM_144966.5
Organism	Homo sapiens(human)
Definition	Homo sapiens FRAS1 related extracellular matrix 1 (FREM1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_144966.5

ORF Insert Sequence:

ATGAACTCTC TGAGTTGGGG GGCTGCGAAT GCCGTGCTGC TGCTGCTCCT CCTGGCCTGG 
GCCAGCCCCA CCTTCATCAG CATCAACCGC GGGGTGAGGG TGATGAAGGG CCACTCTGCC 
TTCCTGTCAG GAGATGACCT GAAGTTTGCC ATCCCTAAAG AGAAAGATGC CTGCAAAGTG 
GAAGTTGTGA TGAATGAGCC AATAACCCAG AGGGTTGGGA AACTCACTCC ACAGGTCTTT 
GACTGCCATT TCCTTCCCAA CGAAGTCAAG TATGTTCACA ATGGTTGTCC AATTCTTGAT 
GAAGACACAG TGAAGCTCAG ACTTTACAGA TTTACTGAAA GAGATACCTT CATAGAAACT 
TTTATCCTGT GGGTCTATCT CCTGGAACCA GACTGTAACA TCATCCATAT GAGTAACAAT 
GTGCTGGAGG TGCCTGAATT CAATGGCTTG TCCCAAGCGA TTGATAAAAA TCTGCTCAGA 
TTCGATTATG ATAGGATGGC TAGCCTGGAA TGTACCGTCA GCCTGGACAC TGCGAGAACT 
CGGCTGCCAG CCCATGGCCA GATGGTTCTC GGGGAGCCTC GACCAGAAGA ACCTCGTGGA 
GATCAGCCAC ACAGTTTTTT CCCAGAGTCC CAACTGAGAG CAAAACTGAA GTGTCCAGGT 
GGGAGCTGTA CCCCAGGATT AAAGAAAATA GGAAGCCTCA AAGTGAGCTG TGAGGAGTTC 
CTGCTGATGG GCCTTCGTTA TCAGCATCTG GATCCCCCTT CACCCAACAT TGATTATATC 
TCCATCCAAC TGGACCTCAC AGATACCAGG AGCAAAATTG TGTACAAGTC AGAGAGTGCG 
TGGCTGCCTG TCTATATCAG AGCTGGAATT CCGAATCAGA TTCCAAAGGC TGCATTCATG 
GCCGTGTTTA TTCTGGAAGT GGATCAGTTC ATCCTGACCT CCTTGACTAC ATCAGTTCTG 
GACTGTGAAG AAGATGAGAC CCCTAAACCC TTGCTGGTGT TCAACATTAC TAAAGCCCCG 
CTCCAGGGCT ATGTGACTCA CCTGTTGGAT CACACCAGAC CAATCTCCTC ATTCACCTGG 
AAAGATCTCA GTGACATGCA GATCGCCTAT CAGCCACCAA ACAGCAGCCA TTCTGAGAGG 
AGACATGATG AGGTAGAATT GGAGGTATAC GACTTCTTCT TTGAAAGGAG TGCACCTATG 
ACAGTCCACA TCTCCATCAG AACAGCAGAT ACAAATGCCC CCCGTGTATC CTGGAATACA 
GGTCTGAGTC TCCTTGAGGG GCAGTCTCGA GCCATCACTT GGGAACAGTT TCAGGTTGTC 
GACAATGACG ACATTGGTGC TGTCCGGCTA GTCACCGTTG GTGGCCTGCA GCATGGATGG 
CTGACTTTAA GAGGGGGGAA AGGGTTTCTC TTCACCGTGG CTGACCTCCA GGCTGGAGTT 
GTTCGCTATC ATCATGATGA CAGCGACTCC ACCAAAGACT TCGTGGTCTT CCGGATATTT 
GATGGCCATC ACAGCATCCG TCACAAATTC CCCATCAACG TCTTGCCCAA AGATGATAGT 
CCCCCGTTCC TCATAACCAA TGTTGTGATT GAACTGGAGG AGGGGCAGAC CATCCTGATC 
CAGGGATCCA TGCTGCGAGC TTCAGATGTG GACGCCAGTG ATGACTACAT CTTCTTCAAT 
ATCACAAAGC CTCCACAGGC TGGGGAGATC ATGAAGAAGC CAGGGCCAGG ACTGATAGGC 
TATCCTGTCC ATGGCTTCCT TCAGAGGGAT TTGTTTAATG GAATCATTTA TTATCGTCAT 
TTTGGTGGAG AAATCTTTGA AGATTCTTTT CAATTTGTCC TGTGGGACAG CCATGAACCT 
CCAAATCTTT CAGTGCCACA GGTGGCAACA ATCCATATAA CTCCAGTGGA TGACCAGCTT 
CCAAAAGAGG CTCCTGGAGT TTCTCGGCAT TTGGTTGTCA AGGAAACTGA GGTGGCCTAT 
ATAACTAAGA AACAGCTACA TTTTATAGAT TCAGAATCAT ATGACAGGGA GCTGGTCTAC 
ACAATAACTA CTCCTCCATT TTTCTCCTTC AGCCACAGAC ACTTGGATGC TGGGAAATTA 
TTTATGGTGG ACAGCATACC AAAAGTAGTT AAGAATCCTA CGGCCCTGGA GCTGAGGTCA 
TTCACTCAGC ATGCTGTGAA CTATATGAAA GTGGCCTACA TGCCCCCCAT GCAAGACATT 
GGTCCCCATT GCAGAGATGT CCAGTTCACA TTTTCTGTCA GTAACCAACA TGGCGGTACT 
TTGCATGGGA TCTGCTTTAA CATTACAATC CTCCCAGTGG ACAATCAAGT TCCTGAGGCG 
TTCACCAACC CTCTGAAAGT GACTGAGGGA GGTCAAAGCA TCATCAGCAC AGAGCACATT 
CTAATTTCTG ATGCAGATAC CAAGCTGGAC AATATTGACC TCTCCCTGCG GGAATTGCCT 
CTGCACGGAA GGGTGGAGCT GAATGGATTT CCTCTAAATT CAGGGGGCAC ATTTTCTTGG 
GGCGATCTCC ATACCTTAAA AGTTAGGTAT CAACATGATG GAACTGAAGT TCTTCAGGAT 
GACCTACTCT TGGAGGTCAC CGATGGCACA AATTCAGCAG AATTTGTACT ACATGTTGAG 
GTATTCCCTG TCAACGATGA GCCACCAGTC TTAAAGGCTG ACCTCATGCC TGTCATGAAT 
TGCTCAGAGG GAGGAGAGGT GGTCATCACC TCTGAATACA TTTTTGCTAC TGATGTGGAC 
AGCGATAACT TGAAGTTGAT GTTTGTGATT GCTCGCGAAC CTCAGCATGG GGTGGTGAGG 
AGAGCTGGAG TCACAGTGGA TCAGTTCTCT CAGAGAGATG TTATCTCAGA GGCCGTGACA 
TACAAACACA CAGGTGGCGA GATTGGCCTG ATGCCTTGTT TTGACACCAT TACATTGGTG 
GTTTCGGATG GAGAGGCTGG CCCTTTTGTG AATGGCTGTT GCTACAATGG ACCCAATCCA 
TCTGTTCCAC TTCATGCGTC CTTTCCAGTG TATGATCTCA ACATCACGGT ATACCCAGTA 
GACAACCAGC CACCTTCCAT TGCAATAGGT CCCGTGTTTG TTGTAGATGA GGGCTGCTCA 
ACAGCCCTTA CTGTTAACCA TTTGTCCGCC ACTGACCCTG ACACTGCAGC AGATGACTTG 
GAATTTGTTT TGGTTTCTCC TCCTCAGTTT GGCTACCTCG AAAATATACT CCCTTCTGTG 
GGTTTTGAAA AAAGCAATAT TGGCATAAGT ATAGATTCAT TTCAGTGGAA AGACATGAAC 
GCTTTTCACA TTAACTATGT GCAGTCCAGG CATCTGAGGA TAGAACCAAC TGCCGACCAG 
TTCACGGTGT ACGTCACAGA TGGGAAGCAT CACTCCTTGG AGATACCATT TTCTATTATA 
ATCAACCCCA CAAATGATGA AGCTCCTGAC TTTGTAGTGC AGAATATTAC TGTGTGTGAG 
GGTCAGATGA AAGAGCTGGA CTCTTCCATC ATCAGCGCTG TGGACCTGGA CATTCCCCAG 
GATGCCCTGC TGTTCAGCAT CACTCAAAAG CCACGCCATG GCCTCCTCAT CGATAGGGGG 
TTTAGCAAAG ACTTCTCTGA GAATAAGCAG CCAGCCAACC CTCACCAGAA ACATGCACCT 
GTTCACAGCT TTTCCATGGA ACTCCTCAAG ACTGGAATGA GGTTGACGTA CATGCATGAT 
GACTCAGAGA GCCTTGCTGA TGATTTTACA ATCCAATTGT CAGATGGGAA ACATAAGATA 
CTTAAAACCA TTTCAGTAGA GGTCATCCCA GTTAATGATG AAAAACCAAT GCTGAGCAAG 
AAGGCTGAAA TTGCAATGAA TATGGGTGAA ACTCGTATTA TTTCCAGTGC TATTCTTTCA 
GCCATAGATG AAGACTCACC CAGGGAGAAG ATTTACTATG TATTTGAAAG GCTTCCCCAA 
AATGGGCAAC TTCAGCTTAA GATAGGGAGG GACTGGGTTC CTCTCTCCCC TGGCATGAAA 
TGCACTCAGG AGGAAGTGGA TCTGAACTTG CTGAGATACA CACACACCGG GGCAATGGAT 
TCCCAGAATC AAGATAGCTT CACCTTCTAC CTTTGGGATG GCAACAACAG GTCCCCTGCT 
CTTGACTGTC AAATCACCAT CAAGGACATG GAAAAAGGTG ATATTGTCAT CCTTACAAAA 
CCACTCGTGG TGTCTAAAGG TGATAGAGGT TTCTTAACAA CCACCACGCT CCTGGCTGTG 
GACGGAACAG ACAAGCCTGA GGAACTGCTC TATGTCATCA CCTCCCCTCC GCGATATGGC 
CAGATCGAAT ATGTTCACTA TCCTGGAGTT CCCATTACAA ACTTCAGCCA AATGGATGTA 
GTGGGGCAGA CAGTTTGCTA TGTACACAAG AGCAAGGTGA CTGTCTCCAG TGACAGATTC 
AGATTCATCA TCAGCAATGG ACTGCGGACC GAGCACGGGG TGTTTGAGAT CACACTGGAG 
ACTGTGGACA GAGCCCTGCC TGTGGTAACC AGGAACAAGG GGTTGAGACT GGCCCAAGGG 
GCCGTGGGCC TGCTTTCCCC TGACCTCCTT CAGCTGACCG ACCCTGATAC ACCTGCGGAG 
AACCTCACCT TCCTCTTGGT TCAGCTCCCC CAGCATGGCC AGCTCTACCT GTGGGGGACA 
GGGCTACTTC AACACAATTT CACCCAGCAG GATGTGGACA GCAAGAATGT GGCCTATCGG 
CACTCAGGAG GGGACTCCCA GACTGACTGC TTTACTTTCA TGGCCACAGA TGGGACAAAC 
CAAGGCTTTA TTGTGAATGG GAGAGTGTGG GAAGAACCTG TTTTATTCAC CATTCAGGTA 
GACCAATTGG ACAAAACAGC TCCTCGTATC ACACTCTTGC ATTCCCCTTC TCAAGTGGGG 
CTCCTGAAAA ATGGCTGCTA CGGGATTTAC ATCACTTCCC GCGTGTTGAA GGCATCAGAC 
CCTGACACTG AGGACGATCA GATCATCTTT AAAATTCTAC AAGGCCCAAA ACATGGACAT 
CTGGAGAACA CAACAACAGG TGAATTTATC CATGAGAAAT TTAGCCAAAA GGACTTAAAC 
AGTAAGACTA TTCTTTACAT CATAAACCCA TCTTTGGAAG TAAATTCAGA TACCGTGGAA 
TTTCAAATCA TGGACCCCAC AGGGAACTCG GCCACTCCTC AAATTTTGGA ACTGAAGTGG 
TCTCATATTG AATGGTCACA GACCGAATAT GAAGTCTGTG AGAATGTGGG TTTGTTGCCC 
TTGGAAATTA TCAGAAGGGG ATATTCCATG GACTCGGCCT TTGTGGGTAT AAAGGTCAAC 
CAAGTGTCAG CTGCAGTTGG AAAAGATTTC ACCGTGATTC CATCTAAACT GATTCAGTTT 
GACCCAGGAA TGTCAACTAA GATGTGGAAT ATAGCAATTA CCTATGACGG ATTAGAGGAA 
GATGATGAGG TCTTTGAAGT AATTCTGAAC TCCCCTGTGA ATGCAGTTCT TGGCACAAAG 
ACAAAAGCTG CAGTGAAAAT TTTGGACTCA AAAGGAGGAC AATGCCATCC TTCATATTCC 
TCCAACCAAA GCAAGCACAG CACATGGGAG AAGGGCATTT GGCATCTGCT GCCCCCAGGG 
TCTTCCTCAT CCACCACTTC TGGTTCCTTT CATCTGGAAA GAAGACCTCT TCCATCTTCC 
ATGCAGCTAG CAGTCATCAG GGGAGACACC CTGCGGGGCT TTGATTCTAC AGATCTTTCT 
CAAAGGAAGC TTAGGACCCG TGGGAATGGC AAAACAGTTC GTCCATCCTC TGTTTATAGA 
AATGGAACAG ACATCATCTA TAATTATCAT GGGATAGTTT CCTTGAAACT GGAGGATGAC 
AGTTTCCCAA CTCACAAAAG GAAGGCCAAA GTATCCATCA TTAGTCAGCC ACAAAAGACA 
ATCAAAGTGG CAGAACTGCC TCAAGCAGAT AAGGTGGAAT CCACAACTGA CTCACACTTC 
CCCAGACAGG ACCAGTTGCC CTCATTTCCA AAGAACTGCA CTCTGGAATT AAAGGGACTC 
TTCCATTTTG AAGAAGGCAT CCAGAAGCTG TATCAGTGCA ATGGGATCGC CTGGAAAGCC 
TGGAGTCCCC AAACCAAGGA TGTGGAAGAC AAATCCTGTC CAGCCGGGTG GCACCAGCAC 
TCAGGCTACT GTCACATCTT GATCACAGAG CAGAAAGGCA CCTGGAATGC GGCTGCCCAA 
GCTTGCAGGG AACAATACCT GGGCAACCTT GTAACTGTAT TCTCCAGGCA GCACATGCGG 
TGGCTCTGGG ACATTGGTGG GAGAAAGTCC TTTTGGATAG GTTTGAACGA CCAAGTGCAT 
GCTGGCCACT GGGAGTGGAT CGGTGGTGAA CCTGTTGCCT TCACCAATGG GAGAAGAGGG 
CCCTCTCAAC GCTCCAAGCT TGGAAAGAGC TGTGTTTTGG TTCAAAGACA AGGGAAATGG 
CAAACAAAAG ACTGTAGGAG AGCCAAACCT CATAATTATG TGTGTTCCAG AAAACTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FREM1 ( XM_005251382.4 ) cDNA ORF clone, Homo sapiens(human) -> XP_005251439.1 Homo sapiens FRAS1 related extracellular matrix 1 (FREM1), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OHu22151
Clone ID Related Accession (Same CDS sequence)	XM_005251382.4 , XM_005251382.3 , NM_144966.5
Accession Version	XM_005251382.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6540bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	FRAS1-related extracellular matrix protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_005251382.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACTCTC TGAGTTGGGG GGCTGCGAAT GCCGTGCTGC TGCTGCTCCT CCTGGCCTGG 
GCCAGCCCCA CCTTCATCAG CATCAACCGC GGGGTGAGGG TGATGAAGGG CCACTCTGCC 
TTCCTGTCAG GAGATGACCT GAAGTTTGCC ATCCCTAAAG AGAAAGATGC CTGCAAAGTG 
GAAGTTGTGA TGAATGAGCC AATAACCCAG AGGGTTGGGA AACTCACTCC ACAGGTCTTT 
GACTGCCATT TCCTTCCCAA CGAAGTCAAG TATGTTCACA ATGGTTGTCC AATTCTTGAT 
GAAGACACAG TGAAGCTCAG ACTTTACAGA TTTACTGAAA GAGATACCTT CATAGAAACT 
TTTATCCTGT GGGTCTATCT CCTGGAACCA GACTGTAACA TCATCCATAT GAGTAACAAT 
GTGCTGGAGG TGCCTGAATT CAATGGCTTG TCCCAAGCGA TTGATAAAAA TCTGCTCAGA 
TTCGATTATG ATAGGATGGC TAGCCTGGAA TGTACCGTCA GCCTGGACAC TGCGAGAACT 
CGGCTGCCAG CCCATGGCCA GATGGTTCTC GGGGAGCCTC GACCAGAAGA ACCTCGTGGA 
GATCAGCCAC ACAGTTTTTT CCCAGAGTCC CAACTGAGAG CAAAACTGAA GTGTCCAGGT 
GGGAGCTGTA CCCCAGGATT AAAGAAAATA GGAAGCCTCA AAGTGAGCTG TGAGGAGTTC 
CTGCTGATGG GCCTTCGTTA TCAGCATCTG GATCCCCCTT CACCCAACAT TGATTATATC 
TCCATCCAAC TGGACCTCAC AGATACCAGG AGCAAAATTG TGTACAAGTC AGAGAGTGCG 
TGGCTGCCTG TCTATATCAG AGCTGGAATT CCGAATCAGA TTCCAAAGGC TGCATTCATG 
GCCGTGTTTA TTCTGGAAGT GGATCAGTTC ATCCTGACCT CCTTGACTAC ATCAGTTCTG 
GACTGTGAAG AAGATGAGAC CCCTAAACCC TTGCTGGTGT TCAACATTAC TAAAGCCCCG 
CTCCAGGGCT ATGTGACTCA CCTGTTGGAT CACACCAGAC CAATCTCCTC ATTCACCTGG 
AAAGATCTCA GTGACATGCA GATCGCCTAT CAGCCACCAA ACAGCAGCCA TTCTGAGAGG 
AGACATGATG AGGTAGAATT GGAGGTATAC GACTTCTTCT TTGAAAGGAG TGCACCTATG 
ACAGTCCACA TCTCCATCAG AACAGCAGAT ACAAATGCCC CCCGTGTATC CTGGAATACA 
GGTCTGAGTC TCCTTGAGGG GCAGTCTCGA GCCATCACTT GGGAACAGTT TCAGGTTGTC 
GACAATGACG ACATTGGTGC TGTCCGGCTA GTCACCGTTG GTGGCCTGCA GCATGGATGG 
CTGACTTTAA GAGGGGGGAA AGGGTTTCTC TTCACCGTGG CTGACCTCCA GGCTGGAGTT 
GTTCGCTATC ATCATGATGA CAGCGACTCC ACCAAAGACT TCGTGGTCTT CCGGATATTT 
GATGGCCATC ACAGCATCCG TCACAAATTC CCCATCAACG TCTTGCCCAA AGATGATAGT 
CCCCCGTTCC TCATAACCAA TGTTGTGATT GAACTGGAGG AGGGGCAGAC CATCCTGATC 
CAGGGATCCA TGCTGCGAGC TTCAGATGTG GACGCCAGTG ATGACTACAT CTTCTTCAAT 
ATCACAAAGC CTCCACAGGC TGGGGAGATC ATGAAGAAGC CAGGGCCAGG ACTGATAGGC 
TATCCTGTCC ATGGCTTCCT TCAGAGGGAT TTGTTTAATG GAATCATTTA TTATCGTCAT 
TTTGGTGGAG AAATCTTTGA AGATTCTTTT CAATTTGTCC TGTGGGACAG CCATGAACCT 
CCAAATCTTT CAGTGCCACA GGTGGCAACA ATCCATATAA CTCCAGTGGA TGACCAGCTT 
CCAAAAGAGG CTCCTGGAGT TTCTCGGCAT TTGGTTGTCA AGGAAACTGA GGTGGCCTAT 
ATAACTAAGA AACAGCTACA TTTTATAGAT TCAGAATCAT ATGACAGGGA GCTGGTCTAC 
ACAATAACTA CTCCTCCATT TTTCTCCTTC AGCCACAGAC ACTTGGATGC TGGGAAATTA 
TTTATGGTGG ACAGCATACC AAAAGTAGTT AAGAATCCTA CGGCCCTGGA GCTGAGGTCA 
TTCACTCAGC ATGCTGTGAA CTATATGAAA GTGGCCTACA TGCCCCCCAT GCAAGACATT 
GGTCCCCATT GCAGAGATGT CCAGTTCACA TTTTCTGTCA GTAACCAACA TGGCGGTACT 
TTGCATGGGA TCTGCTTTAA CATTACAATC CTCCCAGTGG ACAATCAAGT TCCTGAGGCG 
TTCACCAACC CTCTGAAAGT GACTGAGGGA GGTCAAAGCA TCATCAGCAC AGAGCACATT 
CTAATTTCTG ATGCAGATAC CAAGCTGGAC AATATTGACC TCTCCCTGCG GGAATTGCCT 
CTGCACGGAA GGGTGGAGCT GAATGGATTT CCTCTAAATT CAGGGGGCAC ATTTTCTTGG 
GGCGATCTCC ATACCTTAAA AGTTAGGTAT CAACATGATG GAACTGAAGT TCTTCAGGAT 
GACCTACTCT TGGAGGTCAC CGATGGCACA AATTCAGCAG AATTTGTACT ACATGTTGAG 
GTATTCCCTG TCAACGATGA GCCACCAGTC TTAAAGGCTG ACCTCATGCC TGTCATGAAT 
TGCTCAGAGG GAGGAGAGGT GGTCATCACC TCTGAATACA TTTTTGCTAC TGATGTGGAC 
AGCGATAACT TGAAGTTGAT GTTTGTGATT GCTCGCGAAC CTCAGCATGG GGTGGTGAGG 
AGAGCTGGAG TCACAGTGGA TCAGTTCTCT CAGAGAGATG TTATCTCAGA GGCCGTGACA 
TACAAACACA CAGGTGGCGA GATTGGCCTG ATGCCTTGTT TTGACACCAT TACATTGGTG 
GTTTCGGATG GAGAGGCTGG CCCTTTTGTG AATGGCTGTT GCTACAATGG ACCCAATCCA 
TCTGTTCCAC TTCATGCGTC CTTTCCAGTG TATGATCTCA ACATCACGGT ATACCCAGTA 
GACAACCAGC CACCTTCCAT TGCAATAGGT CCCGTGTTTG TTGTAGATGA GGGCTGCTCA 
ACAGCCCTTA CTGTTAACCA TTTGTCCGCC ACTGACCCTG ACACTGCAGC AGATGACTTG 
GAATTTGTTT TGGTTTCTCC TCCTCAGTTT GGCTACCTCG AAAATATACT CCCTTCTGTG 
GGTTTTGAAA AAAGCAATAT TGGCATAAGT ATAGATTCAT TTCAGTGGAA AGACATGAAC 
GCTTTTCACA TTAACTATGT GCAGTCCAGG CATCTGAGGA TAGAACCAAC TGCCGACCAG 
TTCACGGTGT ACGTCACAGA TGGGAAGCAT CACTCCTTGG AGATACCATT TTCTATTATA 
ATCAACCCCA CAAATGATGA AGCTCCTGAC TTTGTAGTGC AGAATATTAC TGTGTGTGAG 
GGTCAGATGA AAGAGCTGGA CTCTTCCATC ATCAGCGCTG TGGACCTGGA CATTCCCCAG 
GATGCCCTGC TGTTCAGCAT CACTCAAAAG CCACGCCATG GCCTCCTCAT CGATAGGGGG 
TTTAGCAAAG ACTTCTCTGA GAATAAGCAG CCAGCCAACC CTCACCAGAA ACATGCACCT 
GTTCACAGCT TTTCCATGGA ACTCCTCAAG ACTGGAATGA GGTTGACGTA CATGCATGAT 
GACTCAGAGA GCCTTGCTGA TGATTTTACA ATCCAATTGT CAGATGGGAA ACATAAGATA 
CTTAAAACCA TTTCAGTAGA GGTCATCCCA GTTAATGATG AAAAACCAAT GCTGAGCAAG 
AAGGCTGAAA TTGCAATGAA TATGGGTGAA ACTCGTATTA TTTCCAGTGC TATTCTTTCA 
GCCATAGATG AAGACTCACC CAGGGAGAAG ATTTACTATG TATTTGAAAG GCTTCCCCAA 
AATGGGCAAC TTCAGCTTAA GATAGGGAGG GACTGGGTTC CTCTCTCCCC TGGCATGAAA 
TGCACTCAGG AGGAAGTGGA TCTGAACTTG CTGAGATACA CACACACCGG GGCAATGGAT 
TCCCAGAATC AAGATAGCTT CACCTTCTAC CTTTGGGATG GCAACAACAG GTCCCCTGCT 
CTTGACTGTC AAATCACCAT CAAGGACATG GAAAAAGGTG ATATTGTCAT CCTTACAAAA 
CCACTCGTGG TGTCTAAAGG TGATAGAGGT TTCTTAACAA CCACCACGCT CCTGGCTGTG 
GACGGAACAG ACAAGCCTGA GGAACTGCTC TATGTCATCA CCTCCCCTCC GCGATATGGC 
CAGATCGAAT ATGTTCACTA TCCTGGAGTT CCCATTACAA ACTTCAGCCA AATGGATGTA 
GTGGGGCAGA CAGTTTGCTA TGTACACAAG AGCAAGGTGA CTGTCTCCAG TGACAGATTC 
AGATTCATCA TCAGCAATGG ACTGCGGACC GAGCACGGGG TGTTTGAGAT CACACTGGAG 
ACTGTGGACA GAGCCCTGCC TGTGGTAACC AGGAACAAGG GGTTGAGACT GGCCCAAGGG 
GCCGTGGGCC TGCTTTCCCC TGACCTCCTT CAGCTGACCG ACCCTGATAC ACCTGCGGAG 
AACCTCACCT TCCTCTTGGT TCAGCTCCCC CAGCATGGCC AGCTCTACCT GTGGGGGACA 
GGGCTACTTC AACACAATTT CACCCAGCAG GATGTGGACA GCAAGAATGT GGCCTATCGG 
CACTCAGGAG GGGACTCCCA GACTGACTGC TTTACTTTCA TGGCCACAGA TGGGACAAAC 
CAAGGCTTTA TTGTGAATGG GAGAGTGTGG GAAGAACCTG TTTTATTCAC CATTCAGGTA 
GACCAATTGG ACAAAACAGC TCCTCGTATC ACACTCTTGC ATTCCCCTTC TCAAGTGGGG 
CTCCTGAAAA ATGGCTGCTA CGGGATTTAC ATCACTTCCC GCGTGTTGAA GGCATCAGAC 
CCTGACACTG AGGACGATCA GATCATCTTT AAAATTCTAC AAGGCCCAAA ACATGGACAT 
CTGGAGAACA CAACAACAGG TGAATTTATC CATGAGAAAT TTAGCCAAAA GGACTTAAAC 
AGTAAGACTA TTCTTTACAT CATAAACCCA TCTTTGGAAG TAAATTCAGA TACCGTGGAA 
TTTCAAATCA TGGACCCCAC AGGGAACTCG GCCACTCCTC AAATTTTGGA ACTGAAGTGG 
TCTCATATTG AATGGTCACA GACCGAATAT GAAGTCTGTG AGAATGTGGG TTTGTTGCCC 
TTGGAAATTA TCAGAAGGGG ATATTCCATG GACTCGGCCT TTGTGGGTAT AAAGGTCAAC 
CAAGTGTCAG CTGCAGTTGG AAAAGATTTC ACCGTGATTC CATCTAAACT GATTCAGTTT 
GACCCAGGAA TGTCAACTAA GATGTGGAAT ATAGCAATTA CCTATGACGG ATTAGAGGAA 
GATGATGAGG TCTTTGAAGT AATTCTGAAC TCCCCTGTGA ATGCAGTTCT TGGCACAAAG 
ACAAAAGCTG CAGTGAAAAT TTTGGACTCA AAAGGAGGAC AATGCCATCC TTCATATTCC 
TCCAACCAAA GCAAGCACAG CACATGGGAG AAGGGCATTT GGCATCTGCT GCCCCCAGGG 
TCTTCCTCAT CCACCACTTC TGGTTCCTTT CATCTGGAAA GAAGACCTCT TCCATCTTCC 
ATGCAGCTAG CAGTCATCAG GGGAGACACC CTGCGGGGCT TTGATTCTAC AGATCTTTCT 
CAAAGGAAGC TTAGGACCCG TGGGAATGGC AAAACAGTTC GTCCATCCTC TGTTTATAGA 
AATGGAACAG ACATCATCTA TAATTATCAT GGGATAGTTT CCTTGAAACT GGAGGATGAC 
AGTTTCCCAA CTCACAAAAG GAAGGCCAAA GTATCCATCA TTAGTCAGCC ACAAAAGACA 
ATCAAAGTGG CAGAACTGCC TCAAGCAGAT AAGGTGGAAT CCACAACTGA CTCACACTTC 
CCCAGACAGG ACCAGTTGCC CTCATTTCCA AAGAACTGCA CTCTGGAATT AAAGGGACTC 
TTCCATTTTG AAGAAGGCAT CCAGAAGCTG TATCAGTGCA ATGGGATCGC CTGGAAAGCC 
TGGAGTCCCC AAACCAAGGA TGTGGAAGAC AAATCCTGTC CAGCCGGGTG GCACCAGCAC 
TCAGGCTACT GTCACATCTT GATCACAGAG CAGAAAGGCA CCTGGAATGC GGCTGCCCAA 
GCTTGCAGGG AACAATACCT GGGCAACCTT GTAACTGTAT TCTCCAGGCA GCACATGCGG 
TGGCTCTGGG ACATTGGTGG GAGAAAGTCC TTTTGGATAG GTTTGAACGA CCAAGTGCAT 
GCTGGCCACT GGGAGTGGAT CGGTGGTGAA CCTGTTGCCT TCACCAATGG GAGAAGAGGG 
CCCTCTCAAC GCTCCAAGCT TGGAAAGAGC TGTGTTTTGG TTCAAAGACA AGGGAAATGG 
CAAACAAAAG ACTGTAGGAG AGCCAAACCT CATAATTATG TGTGTTCCAG AAAACTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005251439.1
CDS	787..7326
Translation	MNSLSWGAANAVLLLLLLAWASPTFISINRGVRVMKGHSAFLSGDDLKFAIPKEKDACKVEVVMNEPITQRVGKLTPQVFDCHFLPNEVKYVHNGCPILDEDTVKLRLYRFTERDTFIETFILWVYLLEPDCNIIHMSNNVLEVPEFNGLSQAIDKNLLRFDYDRMASLECTVSLDTARTRLPAHGQMVLGEPRPEEPRGDQPHSFFPESQLRAKLKCPGGSCTPGLKKIGSLKVSCEEFLLMGLRYQHLDPPSPNIDYISIQLDLTDTRSKIVYKSESAWLPVYIRAGIPNQIPKAAFMAVFILEVDQFILTSLTTSVLDCEEDETPKPLLVFNITKAPLQGYVTHLLDHTRPISSFTWKDLSDMQIAYQPPNSSHSERRHDEVELEVYDFFFERSAPMTVHISIRTADTNAPRVSWNTGLSLLEGQSRAITWEQFQVVDNDDIGAVRLVTVGGLQHGWLTLRGGKGFLFTVADLQAGVVRYHHDDSDSTKDFVVFRIFDGHHSIRHKFPINVLPKDDSPPFLITNVVIELEEGQTILIQGSMLRASDVDASDDYIFFNITKPPQAGEIMKKPGPGLIGYPVHGFLQRDLFNGIIYYRHFGGEIFEDSFQFVLWDSHEPPNLSVPQVATIHITPVDDQLPKEAPGVSRHLVVKETEVAYITKKQLHFIDSESYDRELVYTITTPPFFSFSHRHLDAGKLFMVDSIPKVVKNPTALELRSFTQHAVNYMKVAYMPPMQDIGPHCRDVQFTFSVSNQHGGTLHGICFNITILPVDNQVPEAFTNPLKVTEGGQSIISTEHILISDADTKLDNIDLSLRELPLHGRVELNGFPLNSGGTFSWGDLHTLKVRYQHDGTEVLQDDLLLEVTDGTNSAEFVLHVEVFPVNDEPPVLKADLMPVMNCSEGGEVVITSEYIFATDVDSDNLKLMFVIAREPQHGVVRRAGVTVDQFSQRDVISEAVTYKHTGGEIGLMPCFDTITLVVSDGEAGPFVNGCCYNGPNPSVPLHASFPVYDLNITVYPVDNQPPSIAIGPVFVVDEGCSTALTVNHLSATDPDTAADDLEFVLVSPPQFGYLENILPSVGFEKSNIGISIDSFQWKDMNAFHINYVQSRHLRIEPTADQFTVYVTDGKHHSLEIPFSIIINPTNDEAPDFVVQNITVCEGQMKELDSSIISAVDLDIPQDALLFSITQKPRHGLLIDRGFSKDFSENKQPANPHQKHAPVHSFSMELLKTGMRLTYMHDDSESLADDFTIQLSDGKHKILKTISVEVIPVNDEKPMLSKKAEIAMNMGETRIISSAILSAIDEDSPREKIYYVFERLPQNGQLQLKIGRDWVPLSPGMKCTQEEVDLNLLRYTHTGAMDSQNQDSFTFYLWDGNNRSPALDCQITIKDMEKGDIVILTKPLVVSKGDRGFLTTTTLLAVDGTDKPEELLYVITSPPRYGQIEYVHYPGVPITNFSQMDVVGQTVCYVHKSKVTVSSDRFRFIISNGLRTEHGVFEITLETVDRALPVVTRNKGLRLAQGAVGLLSPDLLQLTDPDTPAENLTFLLVQLPQHGQLYLWGTGLLQHNFTQQDVDSKNVAYRHSGGDSQTDCFTFMATDGTNQGFIVNGRVWEEPVLFTIQVDQLDKTAPRITLLHSPSQVGLLKNGCYGIYITSRVLKASDPDTEDDQIIFKILQGPKHGHLENTTTGEFIHEKFSQKDLNSKTILYIINPSLEVNSDTVEFQIMDPTGNSATPQILELKWSHIEWSQTEYEVCENVGLLPLEIIRRGYSMDSAFVGIKVNQVSAAVGKDFTVIPSKLIQFDPGMSTKMWNIAITYDGLEEDDEVFEVILNSPVNAVLGTKTKAAVKILDSKGGQCHPSYSSNQSKHSTWEKGIWHLLPPGSSSSTTSGSFHLERRPLPSSMQLAVIRGDTLRGFDSTDLSQRKLRTRGNGKTVRPSSVYRNGTDIIYNYHGIVSLKLEDDSFPTHKRKAKVSIISQPQKTIKVAELPQADKVESTTDSHFPRQDQLPSFPKNCTLELKGLFHFEEGIQKLYQCNGIAWKAWSPQTKDVEDKSCPAGWHQHSGYCHILITEQKGTWNAAAQACREQYLGNLVTVFSRQHMRWLWDIGGRKSFWIGLNDQVHAGHWEWIGGEPVAFTNGRRGPSQRSKLGKSCVLVQRQGKWQTKDCRRAKPHNYVCSRKL

Target ORF information:

RefSeq Version	XM_005251382.4
Organism	Homo sapiens(human)
Definition	Homo sapiens FRAS1 related extracellular matrix 1 (FREM1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005251382.4

ORF Insert Sequence:

ATGAACTCTC TGAGTTGGGG GGCTGCGAAT GCCGTGCTGC TGCTGCTCCT CCTGGCCTGG 
GCCAGCCCCA CCTTCATCAG CATCAACCGC GGGGTGAGGG TGATGAAGGG CCACTCTGCC 
TTCCTGTCAG GAGATGACCT GAAGTTTGCC ATCCCTAAAG AGAAAGATGC CTGCAAAGTG 
GAAGTTGTGA TGAATGAGCC AATAACCCAG AGGGTTGGGA AACTCACTCC ACAGGTCTTT 
GACTGCCATT TCCTTCCCAA CGAAGTCAAG TATGTTCACA ATGGTTGTCC AATTCTTGAT 
GAAGACACAG TGAAGCTCAG ACTTTACAGA TTTACTGAAA GAGATACCTT CATAGAAACT 
TTTATCCTGT GGGTCTATCT CCTGGAACCA GACTGTAACA TCATCCATAT GAGTAACAAT 
GTGCTGGAGG TGCCTGAATT CAATGGCTTG TCCCAAGCGA TTGATAAAAA TCTGCTCAGA 
TTCGATTATG ATAGGATGGC TAGCCTGGAA TGTACCGTCA GCCTGGACAC TGCGAGAACT 
CGGCTGCCAG CCCATGGCCA GATGGTTCTC GGGGAGCCTC GACCAGAAGA ACCTCGTGGA 
GATCAGCCAC ACAGTTTTTT CCCAGAGTCC CAACTGAGAG CAAAACTGAA GTGTCCAGGT 
GGGAGCTGTA CCCCAGGATT AAAGAAAATA GGAAGCCTCA AAGTGAGCTG TGAGGAGTTC 
CTGCTGATGG GCCTTCGTTA TCAGCATCTG GATCCCCCTT CACCCAACAT TGATTATATC 
TCCATCCAAC TGGACCTCAC AGATACCAGG AGCAAAATTG TGTACAAGTC AGAGAGTGCG 
TGGCTGCCTG TCTATATCAG AGCTGGAATT CCGAATCAGA TTCCAAAGGC TGCATTCATG 
GCCGTGTTTA TTCTGGAAGT GGATCAGTTC ATCCTGACCT CCTTGACTAC ATCAGTTCTG 
GACTGTGAAG AAGATGAGAC CCCTAAACCC TTGCTGGTGT TCAACATTAC TAAAGCCCCG 
CTCCAGGGCT ATGTGACTCA CCTGTTGGAT CACACCAGAC CAATCTCCTC ATTCACCTGG 
AAAGATCTCA GTGACATGCA GATCGCCTAT CAGCCACCAA ACAGCAGCCA TTCTGAGAGG 
AGACATGATG AGGTAGAATT GGAGGTATAC GACTTCTTCT TTGAAAGGAG TGCACCTATG 
ACAGTCCACA TCTCCATCAG AACAGCAGAT ACAAATGCCC CCCGTGTATC CTGGAATACA 
GGTCTGAGTC TCCTTGAGGG GCAGTCTCGA GCCATCACTT GGGAACAGTT TCAGGTTGTC 
GACAATGACG ACATTGGTGC TGTCCGGCTA GTCACCGTTG GTGGCCTGCA GCATGGATGG 
CTGACTTTAA GAGGGGGGAA AGGGTTTCTC TTCACCGTGG CTGACCTCCA GGCTGGAGTT 
GTTCGCTATC ATCATGATGA CAGCGACTCC ACCAAAGACT TCGTGGTCTT CCGGATATTT 
GATGGCCATC ACAGCATCCG TCACAAATTC CCCATCAACG TCTTGCCCAA AGATGATAGT 
CCCCCGTTCC TCATAACCAA TGTTGTGATT GAACTGGAGG AGGGGCAGAC CATCCTGATC 
CAGGGATCCA TGCTGCGAGC TTCAGATGTG GACGCCAGTG ATGACTACAT CTTCTTCAAT 
ATCACAAAGC CTCCACAGGC TGGGGAGATC ATGAAGAAGC CAGGGCCAGG ACTGATAGGC 
TATCCTGTCC ATGGCTTCCT TCAGAGGGAT TTGTTTAATG GAATCATTTA TTATCGTCAT 
TTTGGTGGAG AAATCTTTGA AGATTCTTTT CAATTTGTCC TGTGGGACAG CCATGAACCT 
CCAAATCTTT CAGTGCCACA GGTGGCAACA ATCCATATAA CTCCAGTGGA TGACCAGCTT 
CCAAAAGAGG CTCCTGGAGT TTCTCGGCAT TTGGTTGTCA AGGAAACTGA GGTGGCCTAT 
ATAACTAAGA AACAGCTACA TTTTATAGAT TCAGAATCAT ATGACAGGGA GCTGGTCTAC 
ACAATAACTA CTCCTCCATT TTTCTCCTTC AGCCACAGAC ACTTGGATGC TGGGAAATTA 
TTTATGGTGG ACAGCATACC AAAAGTAGTT AAGAATCCTA CGGCCCTGGA GCTGAGGTCA 
TTCACTCAGC ATGCTGTGAA CTATATGAAA GTGGCCTACA TGCCCCCCAT GCAAGACATT 
GGTCCCCATT GCAGAGATGT CCAGTTCACA TTTTCTGTCA GTAACCAACA TGGCGGTACT 
TTGCATGGGA TCTGCTTTAA CATTACAATC CTCCCAGTGG ACAATCAAGT TCCTGAGGCG 
TTCACCAACC CTCTGAAAGT GACTGAGGGA GGTCAAAGCA TCATCAGCAC AGAGCACATT 
CTAATTTCTG ATGCAGATAC CAAGCTGGAC AATATTGACC TCTCCCTGCG GGAATTGCCT 
CTGCACGGAA GGGTGGAGCT GAATGGATTT CCTCTAAATT CAGGGGGCAC ATTTTCTTGG 
GGCGATCTCC ATACCTTAAA AGTTAGGTAT CAACATGATG GAACTGAAGT TCTTCAGGAT 
GACCTACTCT TGGAGGTCAC CGATGGCACA AATTCAGCAG AATTTGTACT ACATGTTGAG 
GTATTCCCTG TCAACGATGA GCCACCAGTC TTAAAGGCTG ACCTCATGCC TGTCATGAAT 
TGCTCAGAGG GAGGAGAGGT GGTCATCACC TCTGAATACA TTTTTGCTAC TGATGTGGAC 
AGCGATAACT TGAAGTTGAT GTTTGTGATT GCTCGCGAAC CTCAGCATGG GGTGGTGAGG 
AGAGCTGGAG TCACAGTGGA TCAGTTCTCT CAGAGAGATG TTATCTCAGA GGCCGTGACA 
TACAAACACA CAGGTGGCGA GATTGGCCTG ATGCCTTGTT TTGACACCAT TACATTGGTG 
GTTTCGGATG GAGAGGCTGG CCCTTTTGTG AATGGCTGTT GCTACAATGG ACCCAATCCA 
TCTGTTCCAC TTCATGCGTC CTTTCCAGTG TATGATCTCA ACATCACGGT ATACCCAGTA 
GACAACCAGC CACCTTCCAT TGCAATAGGT CCCGTGTTTG TTGTAGATGA GGGCTGCTCA 
ACAGCCCTTA CTGTTAACCA TTTGTCCGCC ACTGACCCTG ACACTGCAGC AGATGACTTG 
GAATTTGTTT TGGTTTCTCC TCCTCAGTTT GGCTACCTCG AAAATATACT CCCTTCTGTG 
GGTTTTGAAA AAAGCAATAT TGGCATAAGT ATAGATTCAT TTCAGTGGAA AGACATGAAC 
GCTTTTCACA TTAACTATGT GCAGTCCAGG CATCTGAGGA TAGAACCAAC TGCCGACCAG 
TTCACGGTGT ACGTCACAGA TGGGAAGCAT CACTCCTTGG AGATACCATT TTCTATTATA 
ATCAACCCCA CAAATGATGA AGCTCCTGAC TTTGTAGTGC AGAATATTAC TGTGTGTGAG 
GGTCAGATGA AAGAGCTGGA CTCTTCCATC ATCAGCGCTG TGGACCTGGA CATTCCCCAG 
GATGCCCTGC TGTTCAGCAT CACTCAAAAG CCACGCCATG GCCTCCTCAT CGATAGGGGG 
TTTAGCAAAG ACTTCTCTGA GAATAAGCAG CCAGCCAACC CTCACCAGAA ACATGCACCT 
GTTCACAGCT TTTCCATGGA ACTCCTCAAG ACTGGAATGA GGTTGACGTA CATGCATGAT 
GACTCAGAGA GCCTTGCTGA TGATTTTACA ATCCAATTGT CAGATGGGAA ACATAAGATA 
CTTAAAACCA TTTCAGTAGA GGTCATCCCA GTTAATGATG AAAAACCAAT GCTGAGCAAG 
AAGGCTGAAA TTGCAATGAA TATGGGTGAA ACTCGTATTA TTTCCAGTGC TATTCTTTCA 
GCCATAGATG AAGACTCACC CAGGGAGAAG ATTTACTATG TATTTGAAAG GCTTCCCCAA 
AATGGGCAAC TTCAGCTTAA GATAGGGAGG GACTGGGTTC CTCTCTCCCC TGGCATGAAA 
TGCACTCAGG AGGAAGTGGA TCTGAACTTG CTGAGATACA CACACACCGG GGCAATGGAT 
TCCCAGAATC AAGATAGCTT CACCTTCTAC CTTTGGGATG GCAACAACAG GTCCCCTGCT 
CTTGACTGTC AAATCACCAT CAAGGACATG GAAAAAGGTG ATATTGTCAT CCTTACAAAA 
CCACTCGTGG TGTCTAAAGG TGATAGAGGT TTCTTAACAA CCACCACGCT CCTGGCTGTG 
GACGGAACAG ACAAGCCTGA GGAACTGCTC TATGTCATCA CCTCCCCTCC GCGATATGGC 
CAGATCGAAT ATGTTCACTA TCCTGGAGTT CCCATTACAA ACTTCAGCCA AATGGATGTA 
GTGGGGCAGA CAGTTTGCTA TGTACACAAG AGCAAGGTGA CTGTCTCCAG TGACAGATTC 
AGATTCATCA TCAGCAATGG ACTGCGGACC GAGCACGGGG TGTTTGAGAT CACACTGGAG 
ACTGTGGACA GAGCCCTGCC TGTGGTAACC AGGAACAAGG GGTTGAGACT GGCCCAAGGG 
GCCGTGGGCC TGCTTTCCCC TGACCTCCTT CAGCTGACCG ACCCTGATAC ACCTGCGGAG 
AACCTCACCT TCCTCTTGGT TCAGCTCCCC CAGCATGGCC AGCTCTACCT GTGGGGGACA 
GGGCTACTTC AACACAATTT CACCCAGCAG GATGTGGACA GCAAGAATGT GGCCTATCGG 
CACTCAGGAG GGGACTCCCA GACTGACTGC TTTACTTTCA TGGCCACAGA TGGGACAAAC 
CAAGGCTTTA TTGTGAATGG GAGAGTGTGG GAAGAACCTG TTTTATTCAC CATTCAGGTA 
GACCAATTGG ACAAAACAGC TCCTCGTATC ACACTCTTGC ATTCCCCTTC TCAAGTGGGG 
CTCCTGAAAA ATGGCTGCTA CGGGATTTAC ATCACTTCCC GCGTGTTGAA GGCATCAGAC 
CCTGACACTG AGGACGATCA GATCATCTTT AAAATTCTAC AAGGCCCAAA ACATGGACAT 
CTGGAGAACA CAACAACAGG TGAATTTATC CATGAGAAAT TTAGCCAAAA GGACTTAAAC 
AGTAAGACTA TTCTTTACAT CATAAACCCA TCTTTGGAAG TAAATTCAGA TACCGTGGAA 
TTTCAAATCA TGGACCCCAC AGGGAACTCG GCCACTCCTC AAATTTTGGA ACTGAAGTGG 
TCTCATATTG AATGGTCACA GACCGAATAT GAAGTCTGTG AGAATGTGGG TTTGTTGCCC 
TTGGAAATTA TCAGAAGGGG ATATTCCATG GACTCGGCCT TTGTGGGTAT AAAGGTCAAC 
CAAGTGTCAG CTGCAGTTGG AAAAGATTTC ACCGTGATTC CATCTAAACT GATTCAGTTT 
GACCCAGGAA TGTCAACTAA GATGTGGAAT ATAGCAATTA CCTATGACGG ATTAGAGGAA 
GATGATGAGG TCTTTGAAGT AATTCTGAAC TCCCCTGTGA ATGCAGTTCT TGGCACAAAG 
ACAAAAGCTG CAGTGAAAAT TTTGGACTCA AAAGGAGGAC AATGCCATCC TTCATATTCC 
TCCAACCAAA GCAAGCACAG CACATGGGAG AAGGGCATTT GGCATCTGCT GCCCCCAGGG 
TCTTCCTCAT CCACCACTTC TGGTTCCTTT CATCTGGAAA GAAGACCTCT TCCATCTTCC 
ATGCAGCTAG CAGTCATCAG GGGAGACACC CTGCGGGGCT TTGATTCTAC AGATCTTTCT 
CAAAGGAAGC TTAGGACCCG TGGGAATGGC AAAACAGTTC GTCCATCCTC TGTTTATAGA 
AATGGAACAG ACATCATCTA TAATTATCAT GGGATAGTTT CCTTGAAACT GGAGGATGAC 
AGTTTCCCAA CTCACAAAAG GAAGGCCAAA GTATCCATCA TTAGTCAGCC ACAAAAGACA 
ATCAAAGTGG CAGAACTGCC TCAAGCAGAT AAGGTGGAAT CCACAACTGA CTCACACTTC 
CCCAGACAGG ACCAGTTGCC CTCATTTCCA AAGAACTGCA CTCTGGAATT AAAGGGACTC 
TTCCATTTTG AAGAAGGCAT CCAGAAGCTG TATCAGTGCA ATGGGATCGC CTGGAAAGCC 
TGGAGTCCCC AAACCAAGGA TGTGGAAGAC AAATCCTGTC CAGCCGGGTG GCACCAGCAC 
TCAGGCTACT GTCACATCTT GATCACAGAG CAGAAAGGCA CCTGGAATGC GGCTGCCCAA 
GCTTGCAGGG AACAATACCT GGGCAACCTT GTAACTGTAT TCTCCAGGCA GCACATGCGG 
TGGCTCTGGG ACATTGGTGG GAGAAAGTCC TTTTGGATAG GTTTGAACGA CCAAGTGCAT 
GCTGGCCACT GGGAGTGGAT CGGTGGTGAA CCTGTTGCCT TCACCAATGG GAGAAGAGGG 
CCCTCTCAAC GCTCCAAGCT TGGAAAGAGC TGTGTTTTGG TTCAAAGACA AGGGAAATGG 
CAAACAAAAG ACTGTAGGAG AGCCAAACCT CATAATTATG TGTGTTCCAG AAAACTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FREM1 ( XM_005251382.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_005251439.1 Homo sapiens FRAS1 related extracellular matrix 1 (FREM1), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OHu22151
Clone ID Related Accession (Same CDS sequence)	XM_005251382.4 , XM_005251382.3 , NM_144966.5
Accession Version	XM_005251382.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6540bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	FRAS1-related extracellular matrix protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005251382.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACTCTC TGAGTTGGGG GGCTGCGAAT GCCGTGCTGC TGCTGCTCCT CCTGGCCTGG 
GCCAGCCCCA CCTTCATCAG CATCAACCGC GGGGTGAGGG TGATGAAGGG CCACTCTGCC 
TTCCTGTCAG GAGATGACCT GAAGTTTGCC ATCCCTAAAG AGAAAGATGC CTGCAAAGTG 
GAAGTTGTGA TGAATGAGCC AATAACCCAG AGGGTTGGGA AACTCACTCC ACAGGTCTTT 
GACTGCCATT TCCTTCCCAA CGAAGTCAAG TATGTTCACA ATGGTTGTCC AATTCTTGAT 
GAAGACACAG TGAAGCTCAG ACTTTACAGA TTTACTGAAA GAGATACCTT CATAGAAACT 
TTTATCCTGT GGGTCTATCT CCTGGAACCA GACTGTAACA TCATCCATAT GAGTAACAAT 
GTGCTGGAGG TGCCTGAATT CAATGGCTTG TCCCAAGCGA TTGATAAAAA TCTGCTCAGA 
TTCGATTATG ATAGGATGGC TAGCCTGGAA TGTACCGTCA GCCTGGACAC TGCGAGAACT 
CGGCTGCCAG CCCATGGCCA GATGGTTCTC GGGGAGCCTC GACCAGAAGA ACCTCGTGGA 
GATCAGCCAC ACAGTTTTTT CCCAGAGTCC CAACTGAGAG CAAAACTGAA GTGTCCAGGT 
GGGAGCTGTA CCCCAGGATT AAAGAAAATA GGAAGCCTCA AAGTGAGCTG TGAGGAGTTC 
CTGCTGATGG GCCTTCGTTA TCAGCATCTG GATCCCCCTT CACCCAACAT TGATTATATC 
TCCATCCAAC TGGACCTCAC AGATACCAGG AGCAAAATTG TGTACAAGTC AGAGAGTGCG 
TGGCTGCCTG TCTATATCAG AGCTGGAATT CCGAATCAGA TTCCAAAGGC TGCATTCATG 
GCCGTGTTTA TTCTGGAAGT GGATCAGTTC ATCCTGACCT CCTTGACTAC ATCAGTTCTG 
GACTGTGAAG AAGATGAGAC CCCTAAACCC TTGCTGGTGT TCAACATTAC TAAAGCCCCG 
CTCCAGGGCT ATGTGACTCA CCTGTTGGAT CACACCAGAC CAATCTCCTC ATTCACCTGG 
AAAGATCTCA GTGACATGCA GATCGCCTAT CAGCCACCAA ACAGCAGCCA TTCTGAGAGG 
AGACATGATG AGGTAGAATT GGAGGTATAC GACTTCTTCT TTGAAAGGAG TGCACCTATG 
ACAGTCCACA TCTCCATCAG AACAGCAGAT ACAAATGCCC CCCGTGTATC CTGGAATACA 
GGTCTGAGTC TCCTTGAGGG GCAGTCTCGA GCCATCACTT GGGAACAGTT TCAGGTTGTC 
GACAATGACG ACATTGGTGC TGTCCGGCTA GTCACCGTTG GTGGCCTGCA GCATGGATGG 
CTGACTTTAA GAGGGGGGAA AGGGTTTCTC TTCACCGTGG CTGACCTCCA GGCTGGAGTT 
GTTCGCTATC ATCATGATGA CAGCGACTCC ACCAAAGACT TCGTGGTCTT CCGGATATTT 
GATGGCCATC ACAGCATCCG TCACAAATTC CCCATCAACG TCTTGCCCAA AGATGATAGT 
CCCCCGTTCC TCATAACCAA TGTTGTGATT GAACTGGAGG AGGGGCAGAC CATCCTGATC 
CAGGGATCCA TGCTGCGAGC TTCAGATGTG GACGCCAGTG ATGACTACAT CTTCTTCAAT 
ATCACAAAGC CTCCACAGGC TGGGGAGATC ATGAAGAAGC CAGGGCCAGG ACTGATAGGC 
TATCCTGTCC ATGGCTTCCT TCAGAGGGAT TTGTTTAATG GAATCATTTA TTATCGTCAT 
TTTGGTGGAG AAATCTTTGA AGATTCTTTT CAATTTGTCC TGTGGGACAG CCATGAACCT 
CCAAATCTTT CAGTGCCACA GGTGGCAACA ATCCATATAA CTCCAGTGGA TGACCAGCTT 
CCAAAAGAGG CTCCTGGAGT TTCTCGGCAT TTGGTTGTCA AGGAAACTGA GGTGGCCTAT 
ATAACTAAGA AACAGCTACA TTTTATAGAT TCAGAATCAT ATGACAGGGA GCTGGTCTAC 
ACAATAACTA CTCCTCCATT TTTCTCCTTC AGCCACAGAC ACTTGGATGC TGGGAAATTA 
TTTATGGTGG ACAGCATACC AAAAGTAGTT AAGAATCCTA CGGCCCTGGA GCTGAGGTCA 
TTCACTCAGC ATGCTGTGAA CTATATGAAA GTGGCCTACA TGCCCCCCAT GCAAGACATT 
GGTCCCCATT GCAGAGATGT CCAGTTCACA TTTTCTGTCA GTAACCAACA TGGCGGTACT 
TTGCATGGGA TCTGCTTTAA CATTACAATC CTCCCAGTGG ACAATCAAGT TCCTGAGGCG 
TTCACCAACC CTCTGAAAGT GACTGAGGGA GGTCAAAGCA TCATCAGCAC AGAGCACATT 
CTAATTTCTG ATGCAGATAC CAAGCTGGAC AATATTGACC TCTCCCTGCG GGAATTGCCT 
CTGCACGGAA GGGTGGAGCT GAATGGATTT CCTCTAAATT CAGGGGGCAC ATTTTCTTGG 
GGCGATCTCC ATACCTTAAA AGTTAGGTAT CAACATGATG GAACTGAAGT TCTTCAGGAT 
GACCTACTCT TGGAGGTCAC CGATGGCACA AATTCAGCAG AATTTGTACT ACATGTTGAG 
GTATTCCCTG TCAACGATGA GCCACCAGTC TTAAAGGCTG ACCTCATGCC TGTCATGAAT 
TGCTCAGAGG GAGGAGAGGT GGTCATCACC TCTGAATACA TTTTTGCTAC TGATGTGGAC 
AGCGATAACT TGAAGTTGAT GTTTGTGATT GCTCGCGAAC CTCAGCATGG GGTGGTGAGG 
AGAGCTGGAG TCACAGTGGA TCAGTTCTCT CAGAGAGATG TTATCTCAGA GGCCGTGACA 
TACAAACACA CAGGTGGCGA GATTGGCCTG ATGCCTTGTT TTGACACCAT TACATTGGTG 
GTTTCGGATG GAGAGGCTGG CCCTTTTGTG AATGGCTGTT GCTACAATGG ACCCAATCCA 
TCTGTTCCAC TTCATGCGTC CTTTCCAGTG TATGATCTCA ACATCACGGT ATACCCAGTA 
GACAACCAGC CACCTTCCAT TGCAATAGGT CCCGTGTTTG TTGTAGATGA GGGCTGCTCA 
ACAGCCCTTA CTGTTAACCA TTTGTCCGCC ACTGACCCTG ACACTGCAGC AGATGACTTG 
GAATTTGTTT TGGTTTCTCC TCCTCAGTTT GGCTACCTCG AAAATATACT CCCTTCTGTG 
GGTTTTGAAA AAAGCAATAT TGGCATAAGT ATAGATTCAT TTCAGTGGAA AGACATGAAC 
GCTTTTCACA TTAACTATGT GCAGTCCAGG CATCTGAGGA TAGAACCAAC TGCCGACCAG 
TTCACGGTGT ACGTCACAGA TGGGAAGCAT CACTCCTTGG AGATACCATT TTCTATTATA 
ATCAACCCCA CAAATGATGA AGCTCCTGAC TTTGTAGTGC AGAATATTAC TGTGTGTGAG 
GGTCAGATGA AAGAGCTGGA CTCTTCCATC ATCAGCGCTG TGGACCTGGA CATTCCCCAG 
GATGCCCTGC TGTTCAGCAT CACTCAAAAG CCACGCCATG GCCTCCTCAT CGATAGGGGG 
TTTAGCAAAG ACTTCTCTGA GAATAAGCAG CCAGCCAACC CTCACCAGAA ACATGCACCT 
GTTCACAGCT TTTCCATGGA ACTCCTCAAG ACTGGAATGA GGTTGACGTA CATGCATGAT 
GACTCAGAGA GCCTTGCTGA TGATTTTACA ATCCAATTGT CAGATGGGAA ACATAAGATA 
CTTAAAACCA TTTCAGTAGA GGTCATCCCA GTTAATGATG AAAAACCAAT GCTGAGCAAG 
AAGGCTGAAA TTGCAATGAA TATGGGTGAA ACTCGTATTA TTTCCAGTGC TATTCTTTCA 
GCCATAGATG AAGACTCACC CAGGGAGAAG ATTTACTATG TATTTGAAAG GCTTCCCCAA 
AATGGGCAAC TTCAGCTTAA GATAGGGAGG GACTGGGTTC CTCTCTCCCC TGGCATGAAA 
TGCACTCAGG AGGAAGTGGA TCTGAACTTG CTGAGATACA CACACACCGG GGCAATGGAT 
TCCCAGAATC AAGATAGCTT CACCTTCTAC CTTTGGGATG GCAACAACAG GTCCCCTGCT 
CTTGACTGTC AAATCACCAT CAAGGACATG GAAAAAGGTG ATATTGTCAT CCTTACAAAA 
CCACTCGTGG TGTCTAAAGG TGATAGAGGT TTCTTAACAA CCACCACGCT CCTGGCTGTG 
GACGGAACAG ACAAGCCTGA GGAACTGCTC TATGTCATCA CCTCCCCTCC GCGATATGGC 
CAGATCGAAT ATGTTCACTA TCCTGGAGTT CCCATTACAA ACTTCAGCCA AATGGATGTA 
GTGGGGCAGA CAGTTTGCTA TGTACACAAG AGCAAGGTGA CTGTCTCCAG TGACAGATTC 
AGATTCATCA TCAGCAATGG ACTGCGGACC GAGCACGGGG TGTTTGAGAT CACACTGGAG 
ACTGTGGACA GAGCCCTGCC TGTGGTAACC AGGAACAAGG GGTTGAGACT GGCCCAAGGG 
GCCGTGGGCC TGCTTTCCCC TGACCTCCTT CAGCTGACCG ACCCTGATAC ACCTGCGGAG 
AACCTCACCT TCCTCTTGGT TCAGCTCCCC CAGCATGGCC AGCTCTACCT GTGGGGGACA 
GGGCTACTTC AACACAATTT CACCCAGCAG GATGTGGACA GCAAGAATGT GGCCTATCGG 
CACTCAGGAG GGGACTCCCA GACTGACTGC TTTACTTTCA TGGCCACAGA TGGGACAAAC 
CAAGGCTTTA TTGTGAATGG GAGAGTGTGG GAAGAACCTG TTTTATTCAC CATTCAGGTA 
GACCAATTGG ACAAAACAGC TCCTCGTATC ACACTCTTGC ATTCCCCTTC TCAAGTGGGG 
CTCCTGAAAA ATGGCTGCTA CGGGATTTAC ATCACTTCCC GCGTGTTGAA GGCATCAGAC 
CCTGACACTG AGGACGATCA GATCATCTTT AAAATTCTAC AAGGCCCAAA ACATGGACAT 
CTGGAGAACA CAACAACAGG TGAATTTATC CATGAGAAAT TTAGCCAAAA GGACTTAAAC 
AGTAAGACTA TTCTTTACAT CATAAACCCA TCTTTGGAAG TAAATTCAGA TACCGTGGAA 
TTTCAAATCA TGGACCCCAC AGGGAACTCG GCCACTCCTC AAATTTTGGA ACTGAAGTGG 
TCTCATATTG AATGGTCACA GACCGAATAT GAAGTCTGTG AGAATGTGGG TTTGTTGCCC 
TTGGAAATTA TCAGAAGGGG ATATTCCATG GACTCGGCCT TTGTGGGTAT AAAGGTCAAC 
CAAGTGTCAG CTGCAGTTGG AAAAGATTTC ACCGTGATTC CATCTAAACT GATTCAGTTT 
GACCCAGGAA TGTCAACTAA GATGTGGAAT ATAGCAATTA CCTATGACGG ATTAGAGGAA 
GATGATGAGG TCTTTGAAGT AATTCTGAAC TCCCCTGTGA ATGCAGTTCT TGGCACAAAG 
ACAAAAGCTG CAGTGAAAAT TTTGGACTCA AAAGGAGGAC AATGCCATCC TTCATATTCC 
TCCAACCAAA GCAAGCACAG CACATGGGAG AAGGGCATTT GGCATCTGCT GCCCCCAGGG 
TCTTCCTCAT CCACCACTTC TGGTTCCTTT CATCTGGAAA GAAGACCTCT TCCATCTTCC 
ATGCAGCTAG CAGTCATCAG GGGAGACACC CTGCGGGGCT TTGATTCTAC AGATCTTTCT 
CAAAGGAAGC TTAGGACCCG TGGGAATGGC AAAACAGTTC GTCCATCCTC TGTTTATAGA 
AATGGAACAG ACATCATCTA TAATTATCAT GGGATAGTTT CCTTGAAACT GGAGGATGAC 
AGTTTCCCAA CTCACAAAAG GAAGGCCAAA GTATCCATCA TTAGTCAGCC ACAAAAGACA 
ATCAAAGTGG CAGAACTGCC TCAAGCAGAT AAGGTGGAAT CCACAACTGA CTCACACTTC 
CCCAGACAGG ACCAGTTGCC CTCATTTCCA AAGAACTGCA CTCTGGAATT AAAGGGACTC 
TTCCATTTTG AAGAAGGCAT CCAGAAGCTG TATCAGTGCA ATGGGATCGC CTGGAAAGCC 
TGGAGTCCCC AAACCAAGGA TGTGGAAGAC AAATCCTGTC CAGCCGGGTG GCACCAGCAC 
TCAGGCTACT GTCACATCTT GATCACAGAG CAGAAAGGCA CCTGGAATGC GGCTGCCCAA 
GCTTGCAGGG AACAATACCT GGGCAACCTT GTAACTGTAT TCTCCAGGCA GCACATGCGG 
TGGCTCTGGG ACATTGGTGG GAGAAAGTCC TTTTGGATAG GTTTGAACGA CCAAGTGCAT 
GCTGGCCACT GGGAGTGGAT CGGTGGTGAA CCTGTTGCCT TCACCAATGG GAGAAGAGGG 
CCCTCTCAAC GCTCCAAGCT TGGAAAGAGC TGTGTTTTGG TTCAAAGACA AGGGAAATGG 
CAAACAAAAG ACTGTAGGAG AGCCAAACCT CATAATTATG TGTGTTCCAG AAAACTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005251439.1
CDS	1065..7604
Translation	MNSLSWGAANAVLLLLLLAWASPTFISINRGVRVMKGHSAFLSGDDLKFAIPKEKDACKVEVVMNEPITQRVGKLTPQVFDCHFLPNEVKYVHNGCPILDEDTVKLRLYRFTERDTFIETFILWVYLLEPDCNIIHMSNNVLEVPEFNGLSQAIDKNLLRFDYDRMASLECTVSLDTARTRLPAHGQMVLGEPRPEEPRGDQPHSFFPESQLRAKLKCPGGSCTPGLKKIGSLKVSCEEFLLMGLRYQHLDPPSPNIDYISIQLDLTDTRSKIVYKSESAWLPVYIRAGIPNQIPKAAFMAVFILEVDQFILTSLTTSVLDCEEDETPKPLLVFNITKAPLQGYVTHLLDHTRPISSFTWKDLSDMQIAYQPPNSSHSERRHDEVELEVYDFFFERSAPMTVHISIRTADTNAPRVSWNTGLSLLEGQSRAITWEQFQVVDNDDIGAVRLVTVGGLQHGWLTLRGGKGFLFTVADLQAGVVRYHHDDSDSTKDFVVFRIFDGHHSIRHKFPINVLPKDDSPPFLITNVVIELEEGQTILIQGSMLRASDVDASDDYIFFNITKPPQAGEIMKKPGPGLIGYPVHGFLQRDLFNGIIYYRHFGGEIFEDSFQFVLWDSHEPPNLSVPQVATIHITPVDDQLPKEAPGVSRHLVVKETEVAYITKKQLHFIDSESYDRELVYTITTPPFFSFSHRHLDAGKLFMVDSIPKVVKNPTALELRSFTQHAVNYMKVAYMPPMQDIGPHCRDVQFTFSVSNQHGGTLHGICFNITILPVDNQVPEAFTNPLKVTEGGQSIISTEHILISDADTKLDNIDLSLRELPLHGRVELNGFPLNSGGTFSWGDLHTLKVRYQHDGTEVLQDDLLLEVTDGTNSAEFVLHVEVFPVNDEPPVLKADLMPVMNCSEGGEVVITSEYIFATDVDSDNLKLMFVIAREPQHGVVRRAGVTVDQFSQRDVISEAVTYKHTGGEIGLMPCFDTITLVVSDGEAGPFVNGCCYNGPNPSVPLHASFPVYDLNITVYPVDNQPPSIAIGPVFVVDEGCSTALTVNHLSATDPDTAADDLEFVLVSPPQFGYLENILPSVGFEKSNIGISIDSFQWKDMNAFHINYVQSRHLRIEPTADQFTVYVTDGKHHSLEIPFSIIINPTNDEAPDFVVQNITVCEGQMKELDSSIISAVDLDIPQDALLFSITQKPRHGLLIDRGFSKDFSENKQPANPHQKHAPVHSFSMELLKTGMRLTYMHDDSESLADDFTIQLSDGKHKILKTISVEVIPVNDEKPMLSKKAEIAMNMGETRIISSAILSAIDEDSPREKIYYVFERLPQNGQLQLKIGRDWVPLSPGMKCTQEEVDLNLLRYTHTGAMDSQNQDSFTFYLWDGNNRSPALDCQITIKDMEKGDIVILTKPLVVSKGDRGFLTTTTLLAVDGTDKPEELLYVITSPPRYGQIEYVHYPGVPITNFSQMDVVGQTVCYVHKSKVTVSSDRFRFIISNGLRTEHGVFEITLETVDRALPVVTRNKGLRLAQGAVGLLSPDLLQLTDPDTPAENLTFLLVQLPQHGQLYLWGTGLLQHNFTQQDVDSKNVAYRHSGGDSQTDCFTFMATDGTNQGFIVNGRVWEEPVLFTIQVDQLDKTAPRITLLHSPSQVGLLKNGCYGIYITSRVLKASDPDTEDDQIIFKILQGPKHGHLENTTTGEFIHEKFSQKDLNSKTILYIINPSLEVNSDTVEFQIMDPTGNSATPQILELKWSHIEWSQTEYEVCENVGLLPLEIIRRGYSMDSAFVGIKVNQVSAAVGKDFTVIPSKLIQFDPGMSTKMWNIAITYDGLEEDDEVFEVILNSPVNAVLGTKTKAAVKILDSKGGQCHPSYSSNQSKHSTWEKGIWHLLPPGSSSSTTSGSFHLERRPLPSSMQLAVIRGDTLRGFDSTDLSQRKLRTRGNGKTVRPSSVYRNGTDIIYNYHGIVSLKLEDDSFPTHKRKAKVSIISQPQKTIKVAELPQADKVESTTDSHFPRQDQLPSFPKNCTLELKGLFHFEEGIQKLYQCNGIAWKAWSPQTKDVEDKSCPAGWHQHSGYCHILITEQKGTWNAAAQACREQYLGNLVTVFSRQHMRWLWDIGGRKSFWIGLNDQVHAGHWEWIGGEPVAFTNGRRGPSQRSKLGKSCVLVQRQGKWQTKDCRRAKPHNYVCSRKL

Target ORF information:

RefSeq Version	XM_005251382.3
Organism	Homo sapiens(human)
Definition	Homo sapiens FRAS1 related extracellular matrix 1 (FREM1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005251382.3

ORF Insert Sequence:

ATGAACTCTC TGAGTTGGGG GGCTGCGAAT GCCGTGCTGC TGCTGCTCCT CCTGGCCTGG 
GCCAGCCCCA CCTTCATCAG CATCAACCGC GGGGTGAGGG TGATGAAGGG CCACTCTGCC 
TTCCTGTCAG GAGATGACCT GAAGTTTGCC ATCCCTAAAG AGAAAGATGC CTGCAAAGTG 
GAAGTTGTGA TGAATGAGCC AATAACCCAG AGGGTTGGGA AACTCACTCC ACAGGTCTTT 
GACTGCCATT TCCTTCCCAA CGAAGTCAAG TATGTTCACA ATGGTTGTCC AATTCTTGAT 
GAAGACACAG TGAAGCTCAG ACTTTACAGA TTTACTGAAA GAGATACCTT CATAGAAACT 
TTTATCCTGT GGGTCTATCT CCTGGAACCA GACTGTAACA TCATCCATAT GAGTAACAAT 
GTGCTGGAGG TGCCTGAATT CAATGGCTTG TCCCAAGCGA TTGATAAAAA TCTGCTCAGA 
TTCGATTATG ATAGGATGGC TAGCCTGGAA TGTACCGTCA GCCTGGACAC TGCGAGAACT 
CGGCTGCCAG CCCATGGCCA GATGGTTCTC GGGGAGCCTC GACCAGAAGA ACCTCGTGGA 
GATCAGCCAC ACAGTTTTTT CCCAGAGTCC CAACTGAGAG CAAAACTGAA GTGTCCAGGT 
GGGAGCTGTA CCCCAGGATT AAAGAAAATA GGAAGCCTCA AAGTGAGCTG TGAGGAGTTC 
CTGCTGATGG GCCTTCGTTA TCAGCATCTG GATCCCCCTT CACCCAACAT TGATTATATC 
TCCATCCAAC TGGACCTCAC AGATACCAGG AGCAAAATTG TGTACAAGTC AGAGAGTGCG 
TGGCTGCCTG TCTATATCAG AGCTGGAATT CCGAATCAGA TTCCAAAGGC TGCATTCATG 
GCCGTGTTTA TTCTGGAAGT GGATCAGTTC ATCCTGACCT CCTTGACTAC ATCAGTTCTG 
GACTGTGAAG AAGATGAGAC CCCTAAACCC TTGCTGGTGT TCAACATTAC TAAAGCCCCG 
CTCCAGGGCT ATGTGACTCA CCTGTTGGAT CACACCAGAC CAATCTCCTC ATTCACCTGG 
AAAGATCTCA GTGACATGCA GATCGCCTAT CAGCCACCAA ACAGCAGCCA TTCTGAGAGG 
AGACATGATG AGGTAGAATT GGAGGTATAC GACTTCTTCT TTGAAAGGAG TGCACCTATG 
ACAGTCCACA TCTCCATCAG AACAGCAGAT ACAAATGCCC CCCGTGTATC CTGGAATACA 
GGTCTGAGTC TCCTTGAGGG GCAGTCTCGA GCCATCACTT GGGAACAGTT TCAGGTTGTC 
GACAATGACG ACATTGGTGC TGTCCGGCTA GTCACCGTTG GTGGCCTGCA GCATGGATGG 
CTGACTTTAA GAGGGGGGAA AGGGTTTCTC TTCACCGTGG CTGACCTCCA GGCTGGAGTT 
GTTCGCTATC ATCATGATGA CAGCGACTCC ACCAAAGACT TCGTGGTCTT CCGGATATTT 
GATGGCCATC ACAGCATCCG TCACAAATTC CCCATCAACG TCTTGCCCAA AGATGATAGT 
CCCCCGTTCC TCATAACCAA TGTTGTGATT GAACTGGAGG AGGGGCAGAC CATCCTGATC 
CAGGGATCCA TGCTGCGAGC TTCAGATGTG GACGCCAGTG ATGACTACAT CTTCTTCAAT 
ATCACAAAGC CTCCACAGGC TGGGGAGATC ATGAAGAAGC CAGGGCCAGG ACTGATAGGC 
TATCCTGTCC ATGGCTTCCT TCAGAGGGAT TTGTTTAATG GAATCATTTA TTATCGTCAT 
TTTGGTGGAG AAATCTTTGA AGATTCTTTT CAATTTGTCC TGTGGGACAG CCATGAACCT 
CCAAATCTTT CAGTGCCACA GGTGGCAACA ATCCATATAA CTCCAGTGGA TGACCAGCTT 
CCAAAAGAGG CTCCTGGAGT TTCTCGGCAT TTGGTTGTCA AGGAAACTGA GGTGGCCTAT 
ATAACTAAGA AACAGCTACA TTTTATAGAT TCAGAATCAT ATGACAGGGA GCTGGTCTAC 
ACAATAACTA CTCCTCCATT TTTCTCCTTC AGCCACAGAC ACTTGGATGC TGGGAAATTA 
TTTATGGTGG ACAGCATACC AAAAGTAGTT AAGAATCCTA CGGCCCTGGA GCTGAGGTCA 
TTCACTCAGC ATGCTGTGAA CTATATGAAA GTGGCCTACA TGCCCCCCAT GCAAGACATT 
GGTCCCCATT GCAGAGATGT CCAGTTCACA TTTTCTGTCA GTAACCAACA TGGCGGTACT 
TTGCATGGGA TCTGCTTTAA CATTACAATC CTCCCAGTGG ACAATCAAGT TCCTGAGGCG 
TTCACCAACC CTCTGAAAGT GACTGAGGGA GGTCAAAGCA TCATCAGCAC AGAGCACATT 
CTAATTTCTG ATGCAGATAC CAAGCTGGAC AATATTGACC TCTCCCTGCG GGAATTGCCT 
CTGCACGGAA GGGTGGAGCT GAATGGATTT CCTCTAAATT CAGGGGGCAC ATTTTCTTGG 
GGCGATCTCC ATACCTTAAA AGTTAGGTAT CAACATGATG GAACTGAAGT TCTTCAGGAT 
GACCTACTCT TGGAGGTCAC CGATGGCACA AATTCAGCAG AATTTGTACT ACATGTTGAG 
GTATTCCCTG TCAACGATGA GCCACCAGTC TTAAAGGCTG ACCTCATGCC TGTCATGAAT 
TGCTCAGAGG GAGGAGAGGT GGTCATCACC TCTGAATACA TTTTTGCTAC TGATGTGGAC 
AGCGATAACT TGAAGTTGAT GTTTGTGATT GCTCGCGAAC CTCAGCATGG GGTGGTGAGG 
AGAGCTGGAG TCACAGTGGA TCAGTTCTCT CAGAGAGATG TTATCTCAGA GGCCGTGACA 
TACAAACACA CAGGTGGCGA GATTGGCCTG ATGCCTTGTT TTGACACCAT TACATTGGTG 
GTTTCGGATG GAGAGGCTGG CCCTTTTGTG AATGGCTGTT GCTACAATGG ACCCAATCCA 
TCTGTTCCAC TTCATGCGTC CTTTCCAGTG TATGATCTCA ACATCACGGT ATACCCAGTA 
GACAACCAGC CACCTTCCAT TGCAATAGGT CCCGTGTTTG TTGTAGATGA GGGCTGCTCA 
ACAGCCCTTA CTGTTAACCA TTTGTCCGCC ACTGACCCTG ACACTGCAGC AGATGACTTG 
GAATTTGTTT TGGTTTCTCC TCCTCAGTTT GGCTACCTCG AAAATATACT CCCTTCTGTG 
GGTTTTGAAA AAAGCAATAT TGGCATAAGT ATAGATTCAT TTCAGTGGAA AGACATGAAC 
GCTTTTCACA TTAACTATGT GCAGTCCAGG CATCTGAGGA TAGAACCAAC TGCCGACCAG 
TTCACGGTGT ACGTCACAGA TGGGAAGCAT CACTCCTTGG AGATACCATT TTCTATTATA 
ATCAACCCCA CAAATGATGA AGCTCCTGAC TTTGTAGTGC AGAATATTAC TGTGTGTGAG 
GGTCAGATGA AAGAGCTGGA CTCTTCCATC ATCAGCGCTG TGGACCTGGA CATTCCCCAG 
GATGCCCTGC TGTTCAGCAT CACTCAAAAG CCACGCCATG GCCTCCTCAT CGATAGGGGG 
TTTAGCAAAG ACTTCTCTGA GAATAAGCAG CCAGCCAACC CTCACCAGAA ACATGCACCT 
GTTCACAGCT TTTCCATGGA ACTCCTCAAG ACTGGAATGA GGTTGACGTA CATGCATGAT 
GACTCAGAGA GCCTTGCTGA TGATTTTACA ATCCAATTGT CAGATGGGAA ACATAAGATA 
CTTAAAACCA TTTCAGTAGA GGTCATCCCA GTTAATGATG AAAAACCAAT GCTGAGCAAG 
AAGGCTGAAA TTGCAATGAA TATGGGTGAA ACTCGTATTA TTTCCAGTGC TATTCTTTCA 
GCCATAGATG AAGACTCACC CAGGGAGAAG ATTTACTATG TATTTGAAAG GCTTCCCCAA 
AATGGGCAAC TTCAGCTTAA GATAGGGAGG GACTGGGTTC CTCTCTCCCC TGGCATGAAA 
TGCACTCAGG AGGAAGTGGA TCTGAACTTG CTGAGATACA CACACACCGG GGCAATGGAT 
TCCCAGAATC AAGATAGCTT CACCTTCTAC CTTTGGGATG GCAACAACAG GTCCCCTGCT 
CTTGACTGTC AAATCACCAT CAAGGACATG GAAAAAGGTG ATATTGTCAT CCTTACAAAA 
CCACTCGTGG TGTCTAAAGG TGATAGAGGT TTCTTAACAA CCACCACGCT CCTGGCTGTG 
GACGGAACAG ACAAGCCTGA GGAACTGCTC TATGTCATCA CCTCCCCTCC GCGATATGGC 
CAGATCGAAT ATGTTCACTA TCCTGGAGTT CCCATTACAA ACTTCAGCCA AATGGATGTA 
GTGGGGCAGA CAGTTTGCTA TGTACACAAG AGCAAGGTGA CTGTCTCCAG TGACAGATTC 
AGATTCATCA TCAGCAATGG ACTGCGGACC GAGCACGGGG TGTTTGAGAT CACACTGGAG 
ACTGTGGACA GAGCCCTGCC TGTGGTAACC AGGAACAAGG GGTTGAGACT GGCCCAAGGG 
GCCGTGGGCC TGCTTTCCCC TGACCTCCTT CAGCTGACCG ACCCTGATAC ACCTGCGGAG 
AACCTCACCT TCCTCTTGGT TCAGCTCCCC CAGCATGGCC AGCTCTACCT GTGGGGGACA 
GGGCTACTTC AACACAATTT CACCCAGCAG GATGTGGACA GCAAGAATGT GGCCTATCGG 
CACTCAGGAG GGGACTCCCA GACTGACTGC TTTACTTTCA TGGCCACAGA TGGGACAAAC 
CAAGGCTTTA TTGTGAATGG GAGAGTGTGG GAAGAACCTG TTTTATTCAC CATTCAGGTA 
GACCAATTGG ACAAAACAGC TCCTCGTATC ACACTCTTGC ATTCCCCTTC TCAAGTGGGG 
CTCCTGAAAA ATGGCTGCTA CGGGATTTAC ATCACTTCCC GCGTGTTGAA GGCATCAGAC 
CCTGACACTG AGGACGATCA GATCATCTTT AAAATTCTAC AAGGCCCAAA ACATGGACAT 
CTGGAGAACA CAACAACAGG TGAATTTATC CATGAGAAAT TTAGCCAAAA GGACTTAAAC 
AGTAAGACTA TTCTTTACAT CATAAACCCA TCTTTGGAAG TAAATTCAGA TACCGTGGAA 
TTTCAAATCA TGGACCCCAC AGGGAACTCG GCCACTCCTC AAATTTTGGA ACTGAAGTGG 
TCTCATATTG AATGGTCACA GACCGAATAT GAAGTCTGTG AGAATGTGGG TTTGTTGCCC 
TTGGAAATTA TCAGAAGGGG ATATTCCATG GACTCGGCCT TTGTGGGTAT AAAGGTCAAC 
CAAGTGTCAG CTGCAGTTGG AAAAGATTTC ACCGTGATTC CATCTAAACT GATTCAGTTT 
GACCCAGGAA TGTCAACTAA GATGTGGAAT ATAGCAATTA CCTATGACGG ATTAGAGGAA 
GATGATGAGG TCTTTGAAGT AATTCTGAAC TCCCCTGTGA ATGCAGTTCT TGGCACAAAG 
ACAAAAGCTG CAGTGAAAAT TTTGGACTCA AAAGGAGGAC AATGCCATCC TTCATATTCC 
TCCAACCAAA GCAAGCACAG CACATGGGAG AAGGGCATTT GGCATCTGCT GCCCCCAGGG 
TCTTCCTCAT CCACCACTTC TGGTTCCTTT CATCTGGAAA GAAGACCTCT TCCATCTTCC 
ATGCAGCTAG CAGTCATCAG GGGAGACACC CTGCGGGGCT TTGATTCTAC AGATCTTTCT 
CAAAGGAAGC TTAGGACCCG TGGGAATGGC AAAACAGTTC GTCCATCCTC TGTTTATAGA 
AATGGAACAG ACATCATCTA TAATTATCAT GGGATAGTTT CCTTGAAACT GGAGGATGAC 
AGTTTCCCAA CTCACAAAAG GAAGGCCAAA GTATCCATCA TTAGTCAGCC ACAAAAGACA 
ATCAAAGTGG CAGAACTGCC TCAAGCAGAT AAGGTGGAAT CCACAACTGA CTCACACTTC 
CCCAGACAGG ACCAGTTGCC CTCATTTCCA AAGAACTGCA CTCTGGAATT AAAGGGACTC 
TTCCATTTTG AAGAAGGCAT CCAGAAGCTG TATCAGTGCA ATGGGATCGC CTGGAAAGCC 
TGGAGTCCCC AAACCAAGGA TGTGGAAGAC AAATCCTGTC CAGCCGGGTG GCACCAGCAC 
TCAGGCTACT GTCACATCTT GATCACAGAG CAGAAAGGCA CCTGGAATGC GGCTGCCCAA 
GCTTGCAGGG AACAATACCT GGGCAACCTT GTAACTGTAT TCTCCAGGCA GCACATGCGG 
TGGCTCTGGG ACATTGGTGG GAGAAAGTCC TTTTGGATAG GTTTGAACGA CCAAGTGCAT 
GCTGGCCACT GGGAGTGGAT CGGTGGTGAA CCTGTTGCCT TCACCAATGG GAGAAGAGGG 
CCCTCTCAAC GCTCCAAGCT TGGAAAGAGC TGTGTTTTGG TTCAAAGACA AGGGAAATGG 
CAAACAAAAG ACTGTAGGAG AGCCAAACCT CATAATTATG TGTGTTCCAG AAAACTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FREM1 ( NM_001370060.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001356989.1 Homo sapiens FRAS1 related extracellular matrix 1 (FREM1), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OHu108040
Clone ID Related Accession (Same CDS sequence)	NM_001370060.1
Accession Version	NM_001370060.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1293bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Homo sapiens(human)
Product	FRAS1-related extracellular matrix protein 1 isoform 4 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL354672.27 and AL512643.9. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.239330.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1966682, SAMEA1968189 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: full length.

ATGAACTCTC TGAGTTGGGG GGCTGCGAAT GCCGTGCTGC TGCTGCTCCT CCTGGCCTGG 
GCCAGCCCCA CCTTCATCAG CATCAACCGC GGGGTGAGGG TGATGAAGGG CCACTCTGCC 
TTCCTGTCAG GAGATGACCT GAAGTTTGCC ATCCCTAAAG AGAAAGATGC CTGCAAAGTG 
GAAGTTGTGA TGAATGAGCC AATAACCCAG AGGGTTGGGA AACTCACTCC ACAGGTCTTT 
GACTGCCATT TCCTTCCCAA CGAAGTCAAG TATGTTCACA ATGGTTGTCC AATTCTTGAT 
GAAGACACAG TGAAGCTCAG ACTTTACAGA TTTACTGAAA GAGATACCTT CATAGAAACT 
TTTATCCTGT GGGTCTATCT CCTGGAACCA GACTGTAACA TCATCCATAT GAGTAACAAT 
GTGCTGGAGG TGCCTGAATT CAATGGCTTG TCCCAAGCGA TTGATAAAAA TCTGCTCAGA 
TTCGATTATG ATAGGATGGC TAGCCTGGAA TGTACCGTCA GCCTGGACAC TGCGAGAACT 
CGGCTGCCAG CCCATGGCCA GATGGTTCTC GGGGAGCCTC GACCAGAAGA ACCTCGTGGA 
GATCAGCCAC ACAGTTTTTT CCCAGAGTCC CGTATGATGT CTTTTAATGC CTTTCTGGAA 
CTGAGAGCAA AACTGAAGTG TCCAGGTGGG AGCTGTACCC CAGGATTAAA GAAAATAGGA 
AGCCTCAAAG TGAGCTGTGA GGAGTTCCTG CTGATGGGCC TTCGTTATCA GCATCTGGAT 
CCCCCTTCAC CCAACATTGA TTATATCTCC ATCCAACTGG ACCTCACAGA TACCAGGAGC 
AAAATTGTGT ACAAGTCAGA GAGTGCGTGG CTGCCTGTCT ATATCAGAGC TGGAATTCCG 
AATCAGATTC CAAAGGCTGC ATTCATGGCC GTGTTTATTC TGGAAGTGGA TCAGTTCATC 
CTGACCTCCT TGACTACATC AGTTCTGGAC TGTGAAGAAG ATGAGACCCC TAAACCCTTG 
CTGGTGTTCA ACATTACTAA AGCCCCGCTC CAGGGCTATG TGACTCACCT GTTGGATCAC 
ACCAGACCAA TCTCCTCATT CACCTGGAAA GATCTCAGTG ACATGCAGAT CGCCTATCAG 
CCACCAAACA GCAGCCATTC TGAGAGGAGA CATGATGAGG TAGAATTGGA GGTATACGAC 
TTCTTCTTTG AAAGGAGTGC ACCTATGACA GTCCACATCT CCATCAGAAC AGCAGATACA 
AATGCCCCCC GTGTATCCTG GAATACAGCG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001356989.1
CDS	783..2075
Translation	MNSLSWGAANAVLLLLLLAWASPTFISINRGVRVMKGHSAFLSGDDLKFAIPKEKDACKVEVVMNEPITQRVGKLTPQVFDCHFLPNEVKYVHNGCPILDEDTVKLRLYRFTERDTFIETFILWVYLLEPDCNIIHMSNNVLEVPEFNGLSQAIDKNLLRFDYDRMASLECTVSLDTARTRLPAHGQMVLGEPRPEEPRGDQPHSFFPESRMMSFNAFLELRAKLKCPGGSCTPGLKKIGSLKVSCEEFLLMGLRYQHLDPPSPNIDYISIQLDLTDTRSKIVYKSESAWLPVYIRAGIPNQIPKAAFMAVFILEVDQFILTSLTTSVLDCEEDETPKPLLVFNITKAPLQGYVTHLLDHTRPISSFTWKDLSDMQIAYQPPNSSHSERRHDEVELEVYDFFFERSAPMTVHISIRTADTNAPRVSWNTA

Target ORF information:

RefSeq Version	NM_001370060.1
Organism	Homo sapiens(human)
Definition	Homo sapiens FRAS1 related extracellular matrix 1 (FREM1), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001370060.1

ORF Insert Sequence:

ATGAACTCTC TGAGTTGGGG GGCTGCGAAT GCCGTGCTGC TGCTGCTCCT CCTGGCCTGG 
GCCAGCCCCA CCTTCATCAG CATCAACCGC GGGGTGAGGG TGATGAAGGG CCACTCTGCC 
TTCCTGTCAG GAGATGACCT GAAGTTTGCC ATCCCTAAAG AGAAAGATGC CTGCAAAGTG 
GAAGTTGTGA TGAATGAGCC AATAACCCAG AGGGTTGGGA AACTCACTCC ACAGGTCTTT 
GACTGCCATT TCCTTCCCAA CGAAGTCAAG TATGTTCACA ATGGTTGTCC AATTCTTGAT 
GAAGACACAG TGAAGCTCAG ACTTTACAGA TTTACTGAAA GAGATACCTT CATAGAAACT 
TTTATCCTGT GGGTCTATCT CCTGGAACCA GACTGTAACA TCATCCATAT GAGTAACAAT 
GTGCTGGAGG TGCCTGAATT CAATGGCTTG TCCCAAGCGA TTGATAAAAA TCTGCTCAGA 
TTCGATTATG ATAGGATGGC TAGCCTGGAA TGTACCGTCA GCCTGGACAC TGCGAGAACT 
CGGCTGCCAG CCCATGGCCA GATGGTTCTC GGGGAGCCTC GACCAGAAGA ACCTCGTGGA 
GATCAGCCAC ACAGTTTTTT CCCAGAGTCC CGTATGATGT CTTTTAATGC CTTTCTGGAA 
CTGAGAGCAA AACTGAAGTG TCCAGGTGGG AGCTGTACCC CAGGATTAAA GAAAATAGGA 
AGCCTCAAAG TGAGCTGTGA GGAGTTCCTG CTGATGGGCC TTCGTTATCA GCATCTGGAT 
CCCCCTTCAC CCAACATTGA TTATATCTCC ATCCAACTGG ACCTCACAGA TACCAGGAGC 
AAAATTGTGT ACAAGTCAGA GAGTGCGTGG CTGCCTGTCT ATATCAGAGC TGGAATTCCG 
AATCAGATTC CAAAGGCTGC ATTCATGGCC GTGTTTATTC TGGAAGTGGA TCAGTTCATC 
CTGACCTCCT TGACTACATC AGTTCTGGAC TGTGAAGAAG ATGAGACCCC TAAACCCTTG 
CTGGTGTTCA ACATTACTAA AGCCCCGCTC CAGGGCTATG TGACTCACCT GTTGGATCAC 
ACCAGACCAA TCTCCTCATT CACCTGGAAA GATCTCAGTG ACATGCAGAT CGCCTATCAG 
CCACCAAACA GCAGCCATTC TGAGAGGAGA CATGATGAGG TAGAATTGGA GGTATACGAC 
TTCTTCTTTG AAAGGAGTGC ACCTATGACA GTCCACATCT CCATCAGAAC AGCAGATACA 
AATGCCCCCC GTGTATCCTG GAATACAGCG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FREM1 ( NM_001370065.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001356994.1 Homo sapiens FRAS1 related extracellular matrix 1 (FREM1), transcript variant 7, mRNA.

Price & Availability↑

CloneID	OHu108042
Clone ID Related Accession (Same CDS sequence)	NM_001370065.1 , NM_001370063.1
Accession Version	NM_001370065.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1266bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Homo sapiens(human)
Product	FRAS1-related extracellular matrix protein 1 isoform 5 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL354672.27 and AL512643.9. Transcript Variant: This variant (7), as well as variant 6, encodes isoform 5. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.112851.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1966682, SAMEA1968189 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: full length.

ATGAACTCTC TGAGTTGGGG GGCTGCGAAT GCCGTGCTGC TGCTGCTCCT CCTGGCCTGG 
GCCAGCCCCA CCTTCATCAG CATCAACCGC GGGGTGAGGG TGATGAAGGG CCACTCTGCC 
TTCCTGTCAG GAGATGACCT GAAGTTTGCC ATCCCTAAAG AGAAAGATGC CTGCAAAGTG 
GAAGTTGTGA TGAATGAGCC AATAACCCAG AGGGTTGGGA AACTCACTCC ACAGGTCTTT 
GACTGCCATT TCCTTCCCAA CGAAGTCAAG TATGTTCACA ATGGTTGTCC AATTCTTGAT 
GAAGACACAG TGAAGCTCAG ACTTTACAGA TTTACTGAAA GAGATACCTT CATAGAAACT 
TTTATCCTGT GGGTCTATCT CCTGGAACCA GACTGTAACA TCATCCATAT GAGTAACAAT 
GTGCTGGAGG TGCCTGAATT CAATGGCTTG TCCCAAGCGA TTGATAAAAA TCTGCTCAGA 
TTCGATTATG ATAGGATGGC TAGCCTGGAA TGTACCGTCA GCCTGGACAC TGCGAGAACT 
CGGCTGCCAG CCCATGGCCA GATGGTTCTC GGGGAGCCTC GACCAGAAGA ACCTCGTGGA 
GATCAGCCAC ACAGTTTTTT CCCAGAGTCC CAACTGAGAG CAAAACTGAA GTGTCCAGGT 
GGGAGCTGTA CCCCAGGATT AAAGAAAATA GGAAGCCTCA AAGTGAGCTG TGAGGAGTTC 
CTGCTGATGG GCCTTCGTTA TCAGCATCTG GATCCCCCTT CACCCAACAT TGATTATATC 
TCCATCCAAC TGGACCTCAC AGATACCAGG AGCAAAATTG TGTACAAGTC AGAGAGTGCG 
TGGCTGCCTG TCTATATCAG AGCTGGAATT CCGAATCAGA TTCCAAAGGC TGCATTCATG 
GCCGTGTTTA TTCTGGAAGT GGATCAGTTC ATCCTGACCT CCTTGACTAC ATCAGTTCTG 
GACTGTGAAG AAGATGAGAC CCCTAAACCC TTGCTGGTGT TCAACATTAC TAAAGCCCCG 
CTCCAGGGCT ATGTGACTCA CCTGTTGGAT CACACCAGAC CAATCTCCTC ATTCACCTGG 
AAAGATCTCA GTGACATGCA GATCGCCTAT CAGCCACCAA ACAGCAGCCA TTCTGAGAGG 
AGACATGATG AGGTAGAATT GGAGGTATAC GACTTCTTCT TTGAAAGGAG TGCACCTATG 
ACAGTCCACA TCTCCATCAG AACAGCAGAT ACAAATGCCC CCCGTGTATC CTGGAATACA 
GCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001356994.1
CDS	575..1840
Translation	MNSLSWGAANAVLLLLLLAWASPTFISINRGVRVMKGHSAFLSGDDLKFAIPKEKDACKVEVVMNEPITQRVGKLTPQVFDCHFLPNEVKYVHNGCPILDEDTVKLRLYRFTERDTFIETFILWVYLLEPDCNIIHMSNNVLEVPEFNGLSQAIDKNLLRFDYDRMASLECTVSLDTARTRLPAHGQMVLGEPRPEEPRGDQPHSFFPESQLRAKLKCPGGSCTPGLKKIGSLKVSCEEFLLMGLRYQHLDPPSPNIDYISIQLDLTDTRSKIVYKSESAWLPVYIRAGIPNQIPKAAFMAVFILEVDQFILTSLTTSVLDCEEDETPKPLLVFNITKAPLQGYVTHLLDHTRPISSFTWKDLSDMQIAYQPPNSSHSERRHDEVELEVYDFFFERSAPMTVHISIRTADTNAPRVSWNTA

Target ORF information:

RefSeq Version	NM_001370065.1
Organism	Homo sapiens(human)
Definition	Homo sapiens FRAS1 related extracellular matrix 1 (FREM1), transcript variant 7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001370065.1

ORF Insert Sequence:

ATGAACTCTC TGAGTTGGGG GGCTGCGAAT GCCGTGCTGC TGCTGCTCCT CCTGGCCTGG 
GCCAGCCCCA CCTTCATCAG CATCAACCGC GGGGTGAGGG TGATGAAGGG CCACTCTGCC 
TTCCTGTCAG GAGATGACCT GAAGTTTGCC ATCCCTAAAG AGAAAGATGC CTGCAAAGTG 
GAAGTTGTGA TGAATGAGCC AATAACCCAG AGGGTTGGGA AACTCACTCC ACAGGTCTTT 
GACTGCCATT TCCTTCCCAA CGAAGTCAAG TATGTTCACA ATGGTTGTCC AATTCTTGAT 
GAAGACACAG TGAAGCTCAG ACTTTACAGA TTTACTGAAA GAGATACCTT CATAGAAACT 
TTTATCCTGT GGGTCTATCT CCTGGAACCA GACTGTAACA TCATCCATAT GAGTAACAAT 
GTGCTGGAGG TGCCTGAATT CAATGGCTTG TCCCAAGCGA TTGATAAAAA TCTGCTCAGA 
TTCGATTATG ATAGGATGGC TAGCCTGGAA TGTACCGTCA GCCTGGACAC TGCGAGAACT 
CGGCTGCCAG CCCATGGCCA GATGGTTCTC GGGGAGCCTC GACCAGAAGA ACCTCGTGGA 
GATCAGCCAC ACAGTTTTTT CCCAGAGTCC CAACTGAGAG CAAAACTGAA GTGTCCAGGT 
GGGAGCTGTA CCCCAGGATT AAAGAAAATA GGAAGCCTCA AAGTGAGCTG TGAGGAGTTC 
CTGCTGATGG GCCTTCGTTA TCAGCATCTG GATCCCCCTT CACCCAACAT TGATTATATC 
TCCATCCAAC TGGACCTCAC AGATACCAGG AGCAAAATTG TGTACAAGTC AGAGAGTGCG 
TGGCTGCCTG TCTATATCAG AGCTGGAATT CCGAATCAGA TTCCAAAGGC TGCATTCATG 
GCCGTGTTTA TTCTGGAAGT GGATCAGTTC ATCCTGACCT CCTTGACTAC ATCAGTTCTG 
GACTGTGAAG AAGATGAGAC CCCTAAACCC TTGCTGGTGT TCAACATTAC TAAAGCCCCG 
CTCCAGGGCT ATGTGACTCA CCTGTTGGAT CACACCAGAC CAATCTCCTC ATTCACCTGG 
AAAGATCTCA GTGACATGCA GATCGCCTAT CAGCCACCAA ACAGCAGCCA TTCTGAGAGG 
AGACATGATG AGGTAGAATT GGAGGTATAC GACTTCTTCT TTGAAAGGAG TGCACCTATG 
ACAGTCCACA TCTCCATCAG AACAGCAGAT ACAAATGCCC CCCGTGTATC CTGGAATACA 
GCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FREM1 ( NM_001370063.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001356992.1 Homo sapiens FRAS1 related extracellular matrix 1 (FREM1), transcript variant 6, mRNA.

Price & Availability↑

CloneID	OHu108042
Clone ID Related Accession (Same CDS sequence)	NM_001370065.1 , NM_001370063.1
Accession Version	NM_001370063.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1266bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Homo sapiens(human)
Product	FRAS1-related extracellular matrix protein 1 isoform 5 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL354672.27 and AL512643.9. Transcript Variant: This variant (6), as well as variant 7, encodes isoform 5. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.157718.1, SRR1660803.115323.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1966682, SAMEA1968189 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: full length.

ATGAACTCTC TGAGTTGGGG GGCTGCGAAT GCCGTGCTGC TGCTGCTCCT CCTGGCCTGG 
GCCAGCCCCA CCTTCATCAG CATCAACCGC GGGGTGAGGG TGATGAAGGG CCACTCTGCC 
TTCCTGTCAG GAGATGACCT GAAGTTTGCC ATCCCTAAAG AGAAAGATGC CTGCAAAGTG 
GAAGTTGTGA TGAATGAGCC AATAACCCAG AGGGTTGGGA AACTCACTCC ACAGGTCTTT 
GACTGCCATT TCCTTCCCAA CGAAGTCAAG TATGTTCACA ATGGTTGTCC AATTCTTGAT 
GAAGACACAG TGAAGCTCAG ACTTTACAGA TTTACTGAAA GAGATACCTT CATAGAAACT 
TTTATCCTGT GGGTCTATCT CCTGGAACCA GACTGTAACA TCATCCATAT GAGTAACAAT 
GTGCTGGAGG TGCCTGAATT CAATGGCTTG TCCCAAGCGA TTGATAAAAA TCTGCTCAGA 
TTCGATTATG ATAGGATGGC TAGCCTGGAA TGTACCGTCA GCCTGGACAC TGCGAGAACT 
CGGCTGCCAG CCCATGGCCA GATGGTTCTC GGGGAGCCTC GACCAGAAGA ACCTCGTGGA 
GATCAGCCAC ACAGTTTTTT CCCAGAGTCC CAACTGAGAG CAAAACTGAA GTGTCCAGGT 
GGGAGCTGTA CCCCAGGATT AAAGAAAATA GGAAGCCTCA AAGTGAGCTG TGAGGAGTTC 
CTGCTGATGG GCCTTCGTTA TCAGCATCTG GATCCCCCTT CACCCAACAT TGATTATATC 
TCCATCCAAC TGGACCTCAC AGATACCAGG AGCAAAATTG TGTACAAGTC AGAGAGTGCG 
TGGCTGCCTG TCTATATCAG AGCTGGAATT CCGAATCAGA TTCCAAAGGC TGCATTCATG 
GCCGTGTTTA TTCTGGAAGT GGATCAGTTC ATCCTGACCT CCTTGACTAC ATCAGTTCTG 
GACTGTGAAG AAGATGAGAC CCCTAAACCC TTGCTGGTGT TCAACATTAC TAAAGCCCCG 
CTCCAGGGCT ATGTGACTCA CCTGTTGGAT CACACCAGAC CAATCTCCTC ATTCACCTGG 
AAAGATCTCA GTGACATGCA GATCGCCTAT CAGCCACCAA ACAGCAGCCA TTCTGAGAGG 
AGACATGATG AGGTAGAATT GGAGGTATAC GACTTCTTCT TTGAAAGGAG TGCACCTATG 
ACAGTCCACA TCTCCATCAG AACAGCAGAT ACAAATGCCC CCCGTGTATC CTGGAATACA 
GCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001356992.1
CDS	783..2048
Translation	MNSLSWGAANAVLLLLLLAWASPTFISINRGVRVMKGHSAFLSGDDLKFAIPKEKDACKVEVVMNEPITQRVGKLTPQVFDCHFLPNEVKYVHNGCPILDEDTVKLRLYRFTERDTFIETFILWVYLLEPDCNIIHMSNNVLEVPEFNGLSQAIDKNLLRFDYDRMASLECTVSLDTARTRLPAHGQMVLGEPRPEEPRGDQPHSFFPESQLRAKLKCPGGSCTPGLKKIGSLKVSCEEFLLMGLRYQHLDPPSPNIDYISIQLDLTDTRSKIVYKSESAWLPVYIRAGIPNQIPKAAFMAVFILEVDQFILTSLTTSVLDCEEDETPKPLLVFNITKAPLQGYVTHLLDHTRPISSFTWKDLSDMQIAYQPPNSSHSERRHDEVELEVYDFFFERSAPMTVHISIRTADTNAPRVSWNTA

Target ORF information:

RefSeq Version	NM_001370063.1
Organism	Homo sapiens(human)
Definition	Homo sapiens FRAS1 related extracellular matrix 1 (FREM1), transcript variant 6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001370063.1

ORF Insert Sequence:

ATGAACTCTC TGAGTTGGGG GGCTGCGAAT GCCGTGCTGC TGCTGCTCCT CCTGGCCTGG 
GCCAGCCCCA CCTTCATCAG CATCAACCGC GGGGTGAGGG TGATGAAGGG CCACTCTGCC 
TTCCTGTCAG GAGATGACCT GAAGTTTGCC ATCCCTAAAG AGAAAGATGC CTGCAAAGTG 
GAAGTTGTGA TGAATGAGCC AATAACCCAG AGGGTTGGGA AACTCACTCC ACAGGTCTTT 
GACTGCCATT TCCTTCCCAA CGAAGTCAAG TATGTTCACA ATGGTTGTCC AATTCTTGAT 
GAAGACACAG TGAAGCTCAG ACTTTACAGA TTTACTGAAA GAGATACCTT CATAGAAACT 
TTTATCCTGT GGGTCTATCT CCTGGAACCA GACTGTAACA TCATCCATAT GAGTAACAAT 
GTGCTGGAGG TGCCTGAATT CAATGGCTTG TCCCAAGCGA TTGATAAAAA TCTGCTCAGA 
TTCGATTATG ATAGGATGGC TAGCCTGGAA TGTACCGTCA GCCTGGACAC TGCGAGAACT 
CGGCTGCCAG CCCATGGCCA GATGGTTCTC GGGGAGCCTC GACCAGAAGA ACCTCGTGGA 
GATCAGCCAC ACAGTTTTTT CCCAGAGTCC CAACTGAGAG CAAAACTGAA GTGTCCAGGT 
GGGAGCTGTA CCCCAGGATT AAAGAAAATA GGAAGCCTCA AAGTGAGCTG TGAGGAGTTC 
CTGCTGATGG GCCTTCGTTA TCAGCATCTG GATCCCCCTT CACCCAACAT TGATTATATC 
TCCATCCAAC TGGACCTCAC AGATACCAGG AGCAAAATTG TGTACAAGTC AGAGAGTGCG 
TGGCTGCCTG TCTATATCAG AGCTGGAATT CCGAATCAGA TTCCAAAGGC TGCATTCATG 
GCCGTGTTTA TTCTGGAAGT GGATCAGTTC ATCCTGACCT CCTTGACTAC ATCAGTTCTG 
GACTGTGAAG AAGATGAGAC CCCTAAACCC TTGCTGGTGT TCAACATTAC TAAAGCCCCG 
CTCCAGGGCT ATGTGACTCA CCTGTTGGAT CACACCAGAC CAATCTCCTC ATTCACCTGG 
AAAGATCTCA GTGACATGCA GATCGCCTAT CAGCCACCAA ACAGCAGCCA TTCTGAGAGG 
AGACATGATG AGGTAGAATT GGAGGTATAC GACTTCTTCT TTGAAAGGAG TGCACCTATG 
ACAGTCCACA TCTCCATCAG AACAGCAGAT ACAAATGCCC CCCGTGTATC CTGGAATACA 
GCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FREM1 ( NM_001177704.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001171175.1 Homo sapiens FRAS1 related extracellular matrix 1 (FREM1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu22950
Clone ID Related Accession (Same CDS sequence)	NM_001177704.1 , NM_001370061.1 , XM_006716729.3 , XM_005251384.4
Accession Version	NM_001177704.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2148bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-03
Organism	Homo sapiens(human)
Product	FRAS1-related extracellular matrix protein 1 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC031064.1, AL512643.9, AB160987.1 and AL390732.10. Transcript Variant: This variant (2) lacks many of the 5' coding exons found in variant 1 and has a different 5' UTR and 5' CDS. The resulting protein, (isoform 2, also known as Toll-like/interleukin-1 receptor regulator, TILRR) has a different N-terminus and is shorter than isoform 1. This isoform has been shown to associate with the extracellular domain of interleukin-1 receptors and magnify inflammatory responses regulated by nuclear factor kappa B. Variants 2 and 5 both encode the same isoform (2). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC031064.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1966682, SAMEA1968189 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGTGACAC AAGAATCCAT GCTGAAGGCT GCCTTGCCTC TCTTTACCAG ATTCATCATC 
AGCAATGGAC TGCGGACCGA GCACGGGGTG TTTGAGATCA CACTGGAGAC TGTGGACAGA 
GCCCTGCCTG TGGTAACCAG GAACAAGGGG TTGAGACTGG CCCAAGGGGC CGTGGGCCTG 
CTTTCCCCTG ACCTCCTTCA GCTGACCGAC CCTGATACAC CTGCGGAGAA CCTCACCTTC 
CTCTTGGTTC AGCTCCCCCA GCATGGCCAG CTCTACCTGT GGGGGACAGG GCTACTTCAA 
CACAATTTCA CCCAGCAGGA TGTGGACAGC AAGAATGTGG CCTATCGGCA CTCAGGAGGG 
GACTCCCAGA CTGACTGCTT TACTTTCATG GCCACAGATG GGACAAACCA AGGCTTTATT 
GTGAATGGGA GAGTGTGGGA AGAACCTGTT TTATTCACCA TTCAGGTAGA CCAATTGGAC 
AAAACAGCTC CTCGTATCAC ACTCTTGCAT TCCCCTTCTC AAGTGGGGCT CCTGAAAAAT 
GGCTGCTACG GGATTTACAT CACTTCCCGC GTGTTGAAGG CATCAGACCC TGACACTGAG 
GACGATCAGA TCATCTTTAA AATTCTACAA GGCCCAAAAC ATGGACATCT GGAGAACACA 
ACAACAGGTG AATTTATCCA TGAGAAATTT AGCCAAAAGG ACTTAAACAG TAAGACTATT 
CTTTACATCA TAAACCCATC TTTGGAAGTA AATTCAGATA CCGTGGAATT TCAAATCATG 
GACCCCACAG GGAACTCGGC CACTCCTCAA ATTTTGGAAC TGAAGTGGTC TCATATTGAA 
TGGTCACAGA CCGAATATGA AGTCTGTGAG AATGTGGGTT TGTTGCCCTT GGAAATTATC 
AGAAGGGGAT ATTCCATGGA CTCGGCCTTT GTGGGTATAA AGGTCAACCA AGTGTCAGCT 
GCAGTTGGAA AAGATTTCAC CGTGATTCCA TCTAAACTGA TTCAGTTTGA CCCAGGAATG 
TCAACTAAGA TGTGGAATAT AGCAATTACC TATGACGGAT TAGAGGAAGA TGATGAGGTC 
TTTGAAGTAA TTCTGAACTC CCCTGTGAAT GCAGTTCTTG GCACAAAGAC AAAAGCTGCA 
GTGAAAATTT TGGACTCAAA AGGAGGACAA TGCCATCCTT CATATTCCTC CAACCAAAGC 
AAGCACAGCA CATGGGAGAA GGGCATTTGG CATCTGCTGC CCCCAGGGTC TTCCTCATCC 
ACCACTTCTG GTTCCTTTCA TCTGGAAAGA AGACCTCTTC CATCTTCCAT GCAGCTAGCA 
GTCATCAGGG GAGACACCCT GCGGGGCTTT GATTCTACAG ATCTTTCTCA AAGGAAGCTT 
AGGACCCGTG GGAATGGCAA AACAGTTCGT CCATCCTCTG TTTATAGAAA TGGAACAGAC 
ATCATCTATA ATTATCATGG GATAGTTTCC TTGAAACTGG AGGATGACAG TTTCCCAACT 
CACAAAAGGA AGGCCAAAGT ATCCATCATT AGTCAGCCAC AAAAGACAAT CAAAGTGGCA 
GAACTGCCTC AAGCAGATAA GGTGGAATCC ACAACTGACT CACACTTCCC CAGACAGGAC 
CAGTTGCCCT CATTTCCAAA GAACTGCACT CTGGAATTAA AGGGACTCTT CCATTTTGAA 
GAAGGCATCC AGAAGCTGTA TCAGTGCAAT GGGATCGCCT GGAAAGCCTG GAGTCCCCAA 
ACCAAGGATG TGGAAGACAA ATCCTGTCCA GCCGGGTGGC ACCAGCACTC AGGCTACTGT 
CACATCTTGA TCACAGAGCA GAAAGGCACC TGGAATGCGG CTGCCCAAGC TTGCAGGGAA 
CAATACCTGG GCAACCTTGT AACTGTATTC TCCAGGCAGC ACATGCGGTG GCTCTGGGAC 
ATTGGTGGGA GAAAGTCCTT TTGGATAGGT TTGAACGACC AAGTGCATGC TGGCCACTGG 
GAGTGGATCG GTGGTGAACC TGTTGCCTTC ACCAATGGGA GAAGAGGGCC CTCTCAACGC 
TCCAAGCTTG GAAAGAGCTG TGTTTTGGTT CAAAGACAAG GGAAATGGCA AACAAAAGAC 
TGTAGGAGAG CCAAACCTCA TAATTATGTG TGTTCCAGAA AACTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001171175.1
CDS	90..2237
Translation	MVTQESMLKAALPLFTRFIISNGLRTEHGVFEITLETVDRALPVVTRNKGLRLAQGAVGLLSPDLLQLTDPDTPAENLTFLLVQLPQHGQLYLWGTGLLQHNFTQQDVDSKNVAYRHSGGDSQTDCFTFMATDGTNQGFIVNGRVWEEPVLFTIQVDQLDKTAPRITLLHSPSQVGLLKNGCYGIYITSRVLKASDPDTEDDQIIFKILQGPKHGHLENTTTGEFIHEKFSQKDLNSKTILYIINPSLEVNSDTVEFQIMDPTGNSATPQILELKWSHIEWSQTEYEVCENVGLLPLEIIRRGYSMDSAFVGIKVNQVSAAVGKDFTVIPSKLIQFDPGMSTKMWNIAITYDGLEEDDEVFEVILNSPVNAVLGTKTKAAVKILDSKGGQCHPSYSSNQSKHSTWEKGIWHLLPPGSSSSTTSGSFHLERRPLPSSMQLAVIRGDTLRGFDSTDLSQRKLRTRGNGKTVRPSSVYRNGTDIIYNYHGIVSLKLEDDSFPTHKRKAKVSIISQPQKTIKVAELPQADKVESTTDSHFPRQDQLPSFPKNCTLELKGLFHFEEGIQKLYQCNGIAWKAWSPQTKDVEDKSCPAGWHQHSGYCHILITEQKGTWNAAAQACREQYLGNLVTVFSRQHMRWLWDIGGRKSFWIGLNDQVHAGHWEWIGGEPVAFTNGRRGPSQRSKLGKSCVLVQRQGKWQTKDCRRAKPHNYVCSRKL

Target ORF information:

RefSeq Version	NM_001177704.1
Organism	Homo sapiens(human)
Definition	Homo sapiens FRAS1 related extracellular matrix 1 (FREM1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001177704.1

ORF Insert Sequence:

ATGGTGACAC AAGAATCCAT GCTGAAGGCT GCCTTGCCTC TCTTTACCAG ATTCATCATC 
AGCAATGGAC TGCGGACCGA GCACGGGGTG TTTGAGATCA CACTGGAGAC TGTGGACAGA 
GCCCTGCCTG TGGTAACCAG GAACAAGGGG TTGAGACTGG CCCAAGGGGC CGTGGGCCTG 
CTTTCCCCTG ACCTCCTTCA GCTGACCGAC CCTGATACAC CTGCGGAGAA CCTCACCTTC 
CTCTTGGTTC AGCTCCCCCA GCATGGCCAG CTCTACCTGT GGGGGACAGG GCTACTTCAA 
CACAATTTCA CCCAGCAGGA TGTGGACAGC AAGAATGTGG CCTATCGGCA CTCAGGAGGG 
GACTCCCAGA CTGACTGCTT TACTTTCATG GCCACAGATG GGACAAACCA AGGCTTTATT 
GTGAATGGGA GAGTGTGGGA AGAACCTGTT TTATTCACCA TTCAGGTAGA CCAATTGGAC 
AAAACAGCTC CTCGTATCAC ACTCTTGCAT TCCCCTTCTC AAGTGGGGCT CCTGAAAAAT 
GGCTGCTACG GGATTTACAT CACTTCCCGC GTGTTGAAGG CATCAGACCC TGACACTGAG 
GACGATCAGA TCATCTTTAA AATTCTACAA GGCCCAAAAC ATGGACATCT GGAGAACACA 
ACAACAGGTG AATTTATCCA TGAGAAATTT AGCCAAAAGG ACTTAAACAG TAAGACTATT 
CTTTACATCA TAAACCCATC TTTGGAAGTA AATTCAGATA CCGTGGAATT TCAAATCATG 
GACCCCACAG GGAACTCGGC CACTCCTCAA ATTTTGGAAC TGAAGTGGTC TCATATTGAA 
TGGTCACAGA CCGAATATGA AGTCTGTGAG AATGTGGGTT TGTTGCCCTT GGAAATTATC 
AGAAGGGGAT ATTCCATGGA CTCGGCCTTT GTGGGTATAA AGGTCAACCA AGTGTCAGCT 
GCAGTTGGAA AAGATTTCAC CGTGATTCCA TCTAAACTGA TTCAGTTTGA CCCAGGAATG 
TCAACTAAGA TGTGGAATAT AGCAATTACC TATGACGGAT TAGAGGAAGA TGATGAGGTC 
TTTGAAGTAA TTCTGAACTC CCCTGTGAAT GCAGTTCTTG GCACAAAGAC AAAAGCTGCA 
GTGAAAATTT TGGACTCAAA AGGAGGACAA TGCCATCCTT CATATTCCTC CAACCAAAGC 
AAGCACAGCA CATGGGAGAA GGGCATTTGG CATCTGCTGC CCCCAGGGTC TTCCTCATCC 
ACCACTTCTG GTTCCTTTCA TCTGGAAAGA AGACCTCTTC CATCTTCCAT GCAGCTAGCA 
GTCATCAGGG GAGACACCCT GCGGGGCTTT GATTCTACAG ATCTTTCTCA AAGGAAGCTT 
AGGACCCGTG GGAATGGCAA AACAGTTCGT CCATCCTCTG TTTATAGAAA TGGAACAGAC 
ATCATCTATA ATTATCATGG GATAGTTTCC TTGAAACTGG AGGATGACAG TTTCCCAACT 
CACAAAAGGA AGGCCAAAGT ATCCATCATT AGTCAGCCAC AAAAGACAAT CAAAGTGGCA 
GAACTGCCTC AAGCAGATAA GGTGGAATCC ACAACTGACT CACACTTCCC CAGACAGGAC 
CAGTTGCCCT CATTTCCAAA GAACTGCACT CTGGAATTAA AGGGACTCTT CCATTTTGAA 
GAAGGCATCC AGAAGCTGTA TCAGTGCAAT GGGATCGCCT GGAAAGCCTG GAGTCCCCAA 
ACCAAGGATG TGGAAGACAA ATCCTGTCCA GCCGGGTGGC ACCAGCACTC AGGCTACTGT 
CACATCTTGA TCACAGAGCA GAAAGGCACC TGGAATGCGG CTGCCCAAGC TTGCAGGGAA 
CAATACCTGG GCAACCTTGT AACTGTATTC TCCAGGCAGC ACATGCGGTG GCTCTGGGAC 
ATTGGTGGGA GAAAGTCCTT TTGGATAGGT TTGAACGACC AAGTGCATGC TGGCCACTGG 
GAGTGGATCG GTGGTGAACC TGTTGCCTTC ACCAATGGGA GAAGAGGGCC CTCTCAACGC 
TCCAAGCTTG GAAAGAGCTG TGTTTTGGTT CAAAGACAAG GGAAATGGCA AACAAAAGAC 
TGTAGGAGAG CCAAACCTCA TAATTATGTG TGTTCCAGAA AACTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FREM1 ( NM_001370061.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001356990.1 Homo sapiens FRAS1 related extracellular matrix 1 (FREM1), transcript variant 5, mRNA.

Price & Availability↑

CloneID	OHu22950
Clone ID Related Accession (Same CDS sequence)	NM_001177704.1 , NM_001370061.1 , XM_006716729.3 , XM_005251384.4
Accession Version	NM_001370061.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2148bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Homo sapiens(human)
Product	FRAS1-related extracellular matrix protein 1 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL512643.9 and AL390732.10. Transcript Variant: This variant (5), as well as variant 2, encodes isoform 2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.39508.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1966682, SAMEA1968189 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: full length.

ATGGTGACAC AAGAATCCAT GCTGAAGGCT GCCTTGCCTC TCTTTACCAG ATTCATCATC 
AGCAATGGAC TGCGGACCGA GCACGGGGTG TTTGAGATCA CACTGGAGAC TGTGGACAGA 
GCCCTGCCTG TGGTAACCAG GAACAAGGGG TTGAGACTGG CCCAAGGGGC CGTGGGCCTG 
CTTTCCCCTG ACCTCCTTCA GCTGACCGAC CCTGATACAC CTGCGGAGAA CCTCACCTTC 
CTCTTGGTTC AGCTCCCCCA GCATGGCCAG CTCTACCTGT GGGGGACAGG GCTACTTCAA 
CACAATTTCA CCCAGCAGGA TGTGGACAGC AAGAATGTGG CCTATCGGCA CTCAGGAGGG 
GACTCCCAGA CTGACTGCTT TACTTTCATG GCCACAGATG GGACAAACCA AGGCTTTATT 
GTGAATGGGA GAGTGTGGGA AGAACCTGTT TTATTCACCA TTCAGGTAGA CCAATTGGAC 
AAAACAGCTC CTCGTATCAC ACTCTTGCAT TCCCCTTCTC AAGTGGGGCT CCTGAAAAAT 
GGCTGCTACG GGATTTACAT CACTTCCCGC GTGTTGAAGG CATCAGACCC TGACACTGAG 
GACGATCAGA TCATCTTTAA AATTCTACAA GGCCCAAAAC ATGGACATCT GGAGAACACA 
ACAACAGGTG AATTTATCCA TGAGAAATTT AGCCAAAAGG ACTTAAACAG TAAGACTATT 
CTTTACATCA TAAACCCATC TTTGGAAGTA AATTCAGATA CCGTGGAATT TCAAATCATG 
GACCCCACAG GGAACTCGGC CACTCCTCAA ATTTTGGAAC TGAAGTGGTC TCATATTGAA 
TGGTCACAGA CCGAATATGA AGTCTGTGAG AATGTGGGTT TGTTGCCCTT GGAAATTATC 
AGAAGGGGAT ATTCCATGGA CTCGGCCTTT GTGGGTATAA AGGTCAACCA AGTGTCAGCT 
GCAGTTGGAA AAGATTTCAC CGTGATTCCA TCTAAACTGA TTCAGTTTGA CCCAGGAATG 
TCAACTAAGA TGTGGAATAT AGCAATTACC TATGACGGAT TAGAGGAAGA TGATGAGGTC 
TTTGAAGTAA TTCTGAACTC CCCTGTGAAT GCAGTTCTTG GCACAAAGAC AAAAGCTGCA 
GTGAAAATTT TGGACTCAAA AGGAGGACAA TGCCATCCTT CATATTCCTC CAACCAAAGC 
AAGCACAGCA CATGGGAGAA GGGCATTTGG CATCTGCTGC CCCCAGGGTC TTCCTCATCC 
ACCACTTCTG GTTCCTTTCA TCTGGAAAGA AGACCTCTTC CATCTTCCAT GCAGCTAGCA 
GTCATCAGGG GAGACACCCT GCGGGGCTTT GATTCTACAG ATCTTTCTCA AAGGAAGCTT 
AGGACCCGTG GGAATGGCAA AACAGTTCGT CCATCCTCTG TTTATAGAAA TGGAACAGAC 
ATCATCTATA ATTATCATGG GATAGTTTCC TTGAAACTGG AGGATGACAG TTTCCCAACT 
CACAAAAGGA AGGCCAAAGT ATCCATCATT AGTCAGCCAC AAAAGACAAT CAAAGTGGCA 
GAACTGCCTC AAGCAGATAA GGTGGAATCC ACAACTGACT CACACTTCCC CAGACAGGAC 
CAGTTGCCCT CATTTCCAAA GAACTGCACT CTGGAATTAA AGGGACTCTT CCATTTTGAA 
GAAGGCATCC AGAAGCTGTA TCAGTGCAAT GGGATCGCCT GGAAAGCCTG GAGTCCCCAA 
ACCAAGGATG TGGAAGACAA ATCCTGTCCA GCCGGGTGGC ACCAGCACTC AGGCTACTGT 
CACATCTTGA TCACAGAGCA GAAAGGCACC TGGAATGCGG CTGCCCAAGC TTGCAGGGAA 
CAATACCTGG GCAACCTTGT AACTGTATTC TCCAGGCAGC ACATGCGGTG GCTCTGGGAC 
ATTGGTGGGA GAAAGTCCTT TTGGATAGGT TTGAACGACC AAGTGCATGC TGGCCACTGG 
GAGTGGATCG GTGGTGAACC TGTTGCCTTC ACCAATGGGA GAAGAGGGCC CTCTCAACGC 
TCCAAGCTTG GAAAGAGCTG TGTTTTGGTT CAAAGACAAG GGAAATGGCA AACAAAAGAC 
TGTAGGAGAG CCAAACCTCA TAATTATGTG TGTTCCAGAA AACTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001356990.1
CDS	510..2657
Translation	MVTQESMLKAALPLFTRFIISNGLRTEHGVFEITLETVDRALPVVTRNKGLRLAQGAVGLLSPDLLQLTDPDTPAENLTFLLVQLPQHGQLYLWGTGLLQHNFTQQDVDSKNVAYRHSGGDSQTDCFTFMATDGTNQGFIVNGRVWEEPVLFTIQVDQLDKTAPRITLLHSPSQVGLLKNGCYGIYITSRVLKASDPDTEDDQIIFKILQGPKHGHLENTTTGEFIHEKFSQKDLNSKTILYIINPSLEVNSDTVEFQIMDPTGNSATPQILELKWSHIEWSQTEYEVCENVGLLPLEIIRRGYSMDSAFVGIKVNQVSAAVGKDFTVIPSKLIQFDPGMSTKMWNIAITYDGLEEDDEVFEVILNSPVNAVLGTKTKAAVKILDSKGGQCHPSYSSNQSKHSTWEKGIWHLLPPGSSSSTTSGSFHLERRPLPSSMQLAVIRGDTLRGFDSTDLSQRKLRTRGNGKTVRPSSVYRNGTDIIYNYHGIVSLKLEDDSFPTHKRKAKVSIISQPQKTIKVAELPQADKVESTTDSHFPRQDQLPSFPKNCTLELKGLFHFEEGIQKLYQCNGIAWKAWSPQTKDVEDKSCPAGWHQHSGYCHILITEQKGTWNAAAQACREQYLGNLVTVFSRQHMRWLWDIGGRKSFWIGLNDQVHAGHWEWIGGEPVAFTNGRRGPSQRSKLGKSCVLVQRQGKWQTKDCRRAKPHNYVCSRKL

Target ORF information:

RefSeq Version	NM_001370061.1
Organism	Homo sapiens(human)
Definition	Homo sapiens FRAS1 related extracellular matrix 1 (FREM1), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001370061.1

ORF Insert Sequence:

ATGGTGACAC AAGAATCCAT GCTGAAGGCT GCCTTGCCTC TCTTTACCAG ATTCATCATC 
AGCAATGGAC TGCGGACCGA GCACGGGGTG TTTGAGATCA CACTGGAGAC TGTGGACAGA 
GCCCTGCCTG TGGTAACCAG GAACAAGGGG TTGAGACTGG CCCAAGGGGC CGTGGGCCTG 
CTTTCCCCTG ACCTCCTTCA GCTGACCGAC CCTGATACAC CTGCGGAGAA CCTCACCTTC 
CTCTTGGTTC AGCTCCCCCA GCATGGCCAG CTCTACCTGT GGGGGACAGG GCTACTTCAA 
CACAATTTCA CCCAGCAGGA TGTGGACAGC AAGAATGTGG CCTATCGGCA CTCAGGAGGG 
GACTCCCAGA CTGACTGCTT TACTTTCATG GCCACAGATG GGACAAACCA AGGCTTTATT 
GTGAATGGGA GAGTGTGGGA AGAACCTGTT TTATTCACCA TTCAGGTAGA CCAATTGGAC 
AAAACAGCTC CTCGTATCAC ACTCTTGCAT TCCCCTTCTC AAGTGGGGCT CCTGAAAAAT 
GGCTGCTACG GGATTTACAT CACTTCCCGC GTGTTGAAGG CATCAGACCC TGACACTGAG 
GACGATCAGA TCATCTTTAA AATTCTACAA GGCCCAAAAC ATGGACATCT GGAGAACACA 
ACAACAGGTG AATTTATCCA TGAGAAATTT AGCCAAAAGG ACTTAAACAG TAAGACTATT 
CTTTACATCA TAAACCCATC TTTGGAAGTA AATTCAGATA CCGTGGAATT TCAAATCATG 
GACCCCACAG GGAACTCGGC CACTCCTCAA ATTTTGGAAC TGAAGTGGTC TCATATTGAA 
TGGTCACAGA CCGAATATGA AGTCTGTGAG AATGTGGGTT TGTTGCCCTT GGAAATTATC 
AGAAGGGGAT ATTCCATGGA CTCGGCCTTT GTGGGTATAA AGGTCAACCA AGTGTCAGCT 
GCAGTTGGAA AAGATTTCAC CGTGATTCCA TCTAAACTGA TTCAGTTTGA CCCAGGAATG 
TCAACTAAGA TGTGGAATAT AGCAATTACC TATGACGGAT TAGAGGAAGA TGATGAGGTC 
TTTGAAGTAA TTCTGAACTC CCCTGTGAAT GCAGTTCTTG GCACAAAGAC AAAAGCTGCA 
GTGAAAATTT TGGACTCAAA AGGAGGACAA TGCCATCCTT CATATTCCTC CAACCAAAGC 
AAGCACAGCA CATGGGAGAA GGGCATTTGG CATCTGCTGC CCCCAGGGTC TTCCTCATCC 
ACCACTTCTG GTTCCTTTCA TCTGGAAAGA AGACCTCTTC CATCTTCCAT GCAGCTAGCA 
GTCATCAGGG GAGACACCCT GCGGGGCTTT GATTCTACAG ATCTTTCTCA AAGGAAGCTT 
AGGACCCGTG GGAATGGCAA AACAGTTCGT CCATCCTCTG TTTATAGAAA TGGAACAGAC 
ATCATCTATA ATTATCATGG GATAGTTTCC TTGAAACTGG AGGATGACAG TTTCCCAACT 
CACAAAAGGA AGGCCAAAGT ATCCATCATT AGTCAGCCAC AAAAGACAAT CAAAGTGGCA 
GAACTGCCTC AAGCAGATAA GGTGGAATCC ACAACTGACT CACACTTCCC CAGACAGGAC 
CAGTTGCCCT CATTTCCAAA GAACTGCACT CTGGAATTAA AGGGACTCTT CCATTTTGAA 
GAAGGCATCC AGAAGCTGTA TCAGTGCAAT GGGATCGCCT GGAAAGCCTG GAGTCCCCAA 
ACCAAGGATG TGGAAGACAA ATCCTGTCCA GCCGGGTGGC ACCAGCACTC AGGCTACTGT 
CACATCTTGA TCACAGAGCA GAAAGGCACC TGGAATGCGG CTGCCCAAGC TTGCAGGGAA 
CAATACCTGG GCAACCTTGT AACTGTATTC TCCAGGCAGC ACATGCGGTG GCTCTGGGAC 
ATTGGTGGGA GAAAGTCCTT TTGGATAGGT TTGAACGACC AAGTGCATGC TGGCCACTGG 
GAGTGGATCG GTGGTGAACC TGTTGCCTTC ACCAATGGGA GAAGAGGGCC CTCTCAACGC 
TCCAAGCTTG GAAAGAGCTG TGTTTTGGTT CAAAGACAAG GGAAATGGCA AACAAAAGAC 
TGTAGGAGAG CCAAACCTCA TAATTATGTG TGTTCCAGAA AACTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FREM1 ( XM_006716729.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_006716792.1 Homo sapiens FRAS1 related extracellular matrix 1 (FREM1), transcript variant X21, mRNA.

Price & Availability↑

CloneID	OHu22950
Clone ID Related Accession (Same CDS sequence)	NM_001177704.1 , NM_001370061.1 , XM_006716729.3 , XM_005251384.4
Accession Version	XM_006716729.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2148bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	FRAS1-related extracellular matrix protein 1 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006716729.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGACAC AAGAATCCAT GCTGAAGGCT GCCTTGCCTC TCTTTACCAG ATTCATCATC 
AGCAATGGAC TGCGGACCGA GCACGGGGTG TTTGAGATCA CACTGGAGAC TGTGGACAGA 
GCCCTGCCTG TGGTAACCAG GAACAAGGGG TTGAGACTGG CCCAAGGGGC CGTGGGCCTG 
CTTTCCCCTG ACCTCCTTCA GCTGACCGAC CCTGATACAC CTGCGGAGAA CCTCACCTTC 
CTCTTGGTTC AGCTCCCCCA GCATGGCCAG CTCTACCTGT GGGGGACAGG GCTACTTCAA 
CACAATTTCA CCCAGCAGGA TGTGGACAGC AAGAATGTGG CCTATCGGCA CTCAGGAGGG 
GACTCCCAGA CTGACTGCTT TACTTTCATG GCCACAGATG GGACAAACCA AGGCTTTATT 
GTGAATGGGA GAGTGTGGGA AGAACCTGTT TTATTCACCA TTCAGGTAGA CCAATTGGAC 
AAAACAGCTC CTCGTATCAC ACTCTTGCAT TCCCCTTCTC AAGTGGGGCT CCTGAAAAAT 
GGCTGCTACG GGATTTACAT CACTTCCCGC GTGTTGAAGG CATCAGACCC TGACACTGAG 
GACGATCAGA TCATCTTTAA AATTCTACAA GGCCCAAAAC ATGGACATCT GGAGAACACA 
ACAACAGGTG AATTTATCCA TGAGAAATTT AGCCAAAAGG ACTTAAACAG TAAGACTATT 
CTTTACATCA TAAACCCATC TTTGGAAGTA AATTCAGATA CCGTGGAATT TCAAATCATG 
GACCCCACAG GGAACTCGGC CACTCCTCAA ATTTTGGAAC TGAAGTGGTC TCATATTGAA 
TGGTCACAGA CCGAATATGA AGTCTGTGAG AATGTGGGTT TGTTGCCCTT GGAAATTATC 
AGAAGGGGAT ATTCCATGGA CTCGGCCTTT GTGGGTATAA AGGTCAACCA AGTGTCAGCT 
GCAGTTGGAA AAGATTTCAC CGTGATTCCA TCTAAACTGA TTCAGTTTGA CCCAGGAATG 
TCAACTAAGA TGTGGAATAT AGCAATTACC TATGACGGAT TAGAGGAAGA TGATGAGGTC 
TTTGAAGTAA TTCTGAACTC CCCTGTGAAT GCAGTTCTTG GCACAAAGAC AAAAGCTGCA 
GTGAAAATTT TGGACTCAAA AGGAGGACAA TGCCATCCTT CATATTCCTC CAACCAAAGC 
AAGCACAGCA CATGGGAGAA GGGCATTTGG CATCTGCTGC CCCCAGGGTC TTCCTCATCC 
ACCACTTCTG GTTCCTTTCA TCTGGAAAGA AGACCTCTTC CATCTTCCAT GCAGCTAGCA 
GTCATCAGGG GAGACACCCT GCGGGGCTTT GATTCTACAG ATCTTTCTCA AAGGAAGCTT 
AGGACCCGTG GGAATGGCAA AACAGTTCGT CCATCCTCTG TTTATAGAAA TGGAACAGAC 
ATCATCTATA ATTATCATGG GATAGTTTCC TTGAAACTGG AGGATGACAG TTTCCCAACT 
CACAAAAGGA AGGCCAAAGT ATCCATCATT AGTCAGCCAC AAAAGACAAT CAAAGTGGCA 
GAACTGCCTC AAGCAGATAA GGTGGAATCC ACAACTGACT CACACTTCCC CAGACAGGAC 
CAGTTGCCCT CATTTCCAAA GAACTGCACT CTGGAATTAA AGGGACTCTT CCATTTTGAA 
GAAGGCATCC AGAAGCTGTA TCAGTGCAAT GGGATCGCCT GGAAAGCCTG GAGTCCCCAA 
ACCAAGGATG TGGAAGACAA ATCCTGTCCA GCCGGGTGGC ACCAGCACTC AGGCTACTGT 
CACATCTTGA TCACAGAGCA GAAAGGCACC TGGAATGCGG CTGCCCAAGC TTGCAGGGAA 
CAATACCTGG GCAACCTTGT AACTGTATTC TCCAGGCAGC ACATGCGGTG GCTCTGGGAC 
ATTGGTGGGA GAAAGTCCTT TTGGATAGGT TTGAACGACC AAGTGCATGC TGGCCACTGG 
GAGTGGATCG GTGGTGAACC TGTTGCCTTC ACCAATGGGA GAAGAGGGCC CTCTCAACGC 
TCCAAGCTTG GAAAGAGCTG TGTTTTGGTT CAAAGACAAG GGAAATGGCA AACAAAAGAC 
TGTAGGAGAG CCAAACCTCA TAATTATGTG TGTTCCAGAA AACTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006716792.1
CDS	748..2895
Translation	MVTQESMLKAALPLFTRFIISNGLRTEHGVFEITLETVDRALPVVTRNKGLRLAQGAVGLLSPDLLQLTDPDTPAENLTFLLVQLPQHGQLYLWGTGLLQHNFTQQDVDSKNVAYRHSGGDSQTDCFTFMATDGTNQGFIVNGRVWEEPVLFTIQVDQLDKTAPRITLLHSPSQVGLLKNGCYGIYITSRVLKASDPDTEDDQIIFKILQGPKHGHLENTTTGEFIHEKFSQKDLNSKTILYIINPSLEVNSDTVEFQIMDPTGNSATPQILELKWSHIEWSQTEYEVCENVGLLPLEIIRRGYSMDSAFVGIKVNQVSAAVGKDFTVIPSKLIQFDPGMSTKMWNIAITYDGLEEDDEVFEVILNSPVNAVLGTKTKAAVKILDSKGGQCHPSYSSNQSKHSTWEKGIWHLLPPGSSSSTTSGSFHLERRPLPSSMQLAVIRGDTLRGFDSTDLSQRKLRTRGNGKTVRPSSVYRNGTDIIYNYHGIVSLKLEDDSFPTHKRKAKVSIISQPQKTIKVAELPQADKVESTTDSHFPRQDQLPSFPKNCTLELKGLFHFEEGIQKLYQCNGIAWKAWSPQTKDVEDKSCPAGWHQHSGYCHILITEQKGTWNAAAQACREQYLGNLVTVFSRQHMRWLWDIGGRKSFWIGLNDQVHAGHWEWIGGEPVAFTNGRRGPSQRSKLGKSCVLVQRQGKWQTKDCRRAKPHNYVCSRKL

Target ORF information:

RefSeq Version	XM_006716729.3
Organism	Homo sapiens(human)
Definition	Homo sapiens FRAS1 related extracellular matrix 1 (FREM1), transcript variant X21, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006716729.3

ORF Insert Sequence:

ATGGTGACAC AAGAATCCAT GCTGAAGGCT GCCTTGCCTC TCTTTACCAG ATTCATCATC 
AGCAATGGAC TGCGGACCGA GCACGGGGTG TTTGAGATCA CACTGGAGAC TGTGGACAGA 
GCCCTGCCTG TGGTAACCAG GAACAAGGGG TTGAGACTGG CCCAAGGGGC CGTGGGCCTG 
CTTTCCCCTG ACCTCCTTCA GCTGACCGAC CCTGATACAC CTGCGGAGAA CCTCACCTTC 
CTCTTGGTTC AGCTCCCCCA GCATGGCCAG CTCTACCTGT GGGGGACAGG GCTACTTCAA 
CACAATTTCA CCCAGCAGGA TGTGGACAGC AAGAATGTGG CCTATCGGCA CTCAGGAGGG 
GACTCCCAGA CTGACTGCTT TACTTTCATG GCCACAGATG GGACAAACCA AGGCTTTATT 
GTGAATGGGA GAGTGTGGGA AGAACCTGTT TTATTCACCA TTCAGGTAGA CCAATTGGAC 
AAAACAGCTC CTCGTATCAC ACTCTTGCAT TCCCCTTCTC AAGTGGGGCT CCTGAAAAAT 
GGCTGCTACG GGATTTACAT CACTTCCCGC GTGTTGAAGG CATCAGACCC TGACACTGAG 
GACGATCAGA TCATCTTTAA AATTCTACAA GGCCCAAAAC ATGGACATCT GGAGAACACA 
ACAACAGGTG AATTTATCCA TGAGAAATTT AGCCAAAAGG ACTTAAACAG TAAGACTATT 
CTTTACATCA TAAACCCATC TTTGGAAGTA AATTCAGATA CCGTGGAATT TCAAATCATG 
GACCCCACAG GGAACTCGGC CACTCCTCAA ATTTTGGAAC TGAAGTGGTC TCATATTGAA 
TGGTCACAGA CCGAATATGA AGTCTGTGAG AATGTGGGTT TGTTGCCCTT GGAAATTATC 
AGAAGGGGAT ATTCCATGGA CTCGGCCTTT GTGGGTATAA AGGTCAACCA AGTGTCAGCT 
GCAGTTGGAA AAGATTTCAC CGTGATTCCA TCTAAACTGA TTCAGTTTGA CCCAGGAATG 
TCAACTAAGA TGTGGAATAT AGCAATTACC TATGACGGAT TAGAGGAAGA TGATGAGGTC 
TTTGAAGTAA TTCTGAACTC CCCTGTGAAT GCAGTTCTTG GCACAAAGAC AAAAGCTGCA 
GTGAAAATTT TGGACTCAAA AGGAGGACAA TGCCATCCTT CATATTCCTC CAACCAAAGC 
AAGCACAGCA CATGGGAGAA GGGCATTTGG CATCTGCTGC CCCCAGGGTC TTCCTCATCC 
ACCACTTCTG GTTCCTTTCA TCTGGAAAGA AGACCTCTTC CATCTTCCAT GCAGCTAGCA 
GTCATCAGGG GAGACACCCT GCGGGGCTTT GATTCTACAG ATCTTTCTCA AAGGAAGCTT 
AGGACCCGTG GGAATGGCAA AACAGTTCGT CCATCCTCTG TTTATAGAAA TGGAACAGAC 
ATCATCTATA ATTATCATGG GATAGTTTCC TTGAAACTGG AGGATGACAG TTTCCCAACT 
CACAAAAGGA AGGCCAAAGT ATCCATCATT AGTCAGCCAC AAAAGACAAT CAAAGTGGCA 
GAACTGCCTC AAGCAGATAA GGTGGAATCC ACAACTGACT CACACTTCCC CAGACAGGAC 
CAGTTGCCCT CATTTCCAAA GAACTGCACT CTGGAATTAA AGGGACTCTT CCATTTTGAA 
GAAGGCATCC AGAAGCTGTA TCAGTGCAAT GGGATCGCCT GGAAAGCCTG GAGTCCCCAA 
ACCAAGGATG TGGAAGACAA ATCCTGTCCA GCCGGGTGGC ACCAGCACTC AGGCTACTGT 
CACATCTTGA TCACAGAGCA GAAAGGCACC TGGAATGCGG CTGCCCAAGC TTGCAGGGAA 
CAATACCTGG GCAACCTTGT AACTGTATTC TCCAGGCAGC ACATGCGGTG GCTCTGGGAC 
ATTGGTGGGA GAAAGTCCTT TTGGATAGGT TTGAACGACC AAGTGCATGC TGGCCACTGG 
GAGTGGATCG GTGGTGAACC TGTTGCCTTC ACCAATGGGA GAAGAGGGCC CTCTCAACGC 
TCCAAGCTTG GAAAGAGCTG TGTTTTGGTT CAAAGACAAG GGAAATGGCA AACAAAAGAC 
TGTAGGAGAG CCAAACCTCA TAATTATGTG TGTTCCAGAA AACTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FREM1 ( XM_005251384.4 ) cDNA ORF clone, Homo sapiens(human) -> XP_005251441.1 Homo sapiens FRAS1 related extracellular matrix 1 (FREM1), transcript variant X22, mRNA.

Price & Availability↑

CloneID	OHu22950
Clone ID Related Accession (Same CDS sequence)	NM_001177704.1 , NM_001370061.1 , XM_006716729.3 , XM_005251384.4
Accession Version	XM_005251384.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2148bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	FRAS1-related extracellular matrix protein 1 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005251384.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGACAC AAGAATCCAT GCTGAAGGCT GCCTTGCCTC TCTTTACCAG ATTCATCATC 
AGCAATGGAC TGCGGACCGA GCACGGGGTG TTTGAGATCA CACTGGAGAC TGTGGACAGA 
GCCCTGCCTG TGGTAACCAG GAACAAGGGG TTGAGACTGG CCCAAGGGGC CGTGGGCCTG 
CTTTCCCCTG ACCTCCTTCA GCTGACCGAC CCTGATACAC CTGCGGAGAA CCTCACCTTC 
CTCTTGGTTC AGCTCCCCCA GCATGGCCAG CTCTACCTGT GGGGGACAGG GCTACTTCAA 
CACAATTTCA CCCAGCAGGA TGTGGACAGC AAGAATGTGG CCTATCGGCA CTCAGGAGGG 
GACTCCCAGA CTGACTGCTT TACTTTCATG GCCACAGATG GGACAAACCA AGGCTTTATT 
GTGAATGGGA GAGTGTGGGA AGAACCTGTT TTATTCACCA TTCAGGTAGA CCAATTGGAC 
AAAACAGCTC CTCGTATCAC ACTCTTGCAT TCCCCTTCTC AAGTGGGGCT CCTGAAAAAT 
GGCTGCTACG GGATTTACAT CACTTCCCGC GTGTTGAAGG CATCAGACCC TGACACTGAG 
GACGATCAGA TCATCTTTAA AATTCTACAA GGCCCAAAAC ATGGACATCT GGAGAACACA 
ACAACAGGTG AATTTATCCA TGAGAAATTT AGCCAAAAGG ACTTAAACAG TAAGACTATT 
CTTTACATCA TAAACCCATC TTTGGAAGTA AATTCAGATA CCGTGGAATT TCAAATCATG 
GACCCCACAG GGAACTCGGC CACTCCTCAA ATTTTGGAAC TGAAGTGGTC TCATATTGAA 
TGGTCACAGA CCGAATATGA AGTCTGTGAG AATGTGGGTT TGTTGCCCTT GGAAATTATC 
AGAAGGGGAT ATTCCATGGA CTCGGCCTTT GTGGGTATAA AGGTCAACCA AGTGTCAGCT 
GCAGTTGGAA AAGATTTCAC CGTGATTCCA TCTAAACTGA TTCAGTTTGA CCCAGGAATG 
TCAACTAAGA TGTGGAATAT AGCAATTACC TATGACGGAT TAGAGGAAGA TGATGAGGTC 
TTTGAAGTAA TTCTGAACTC CCCTGTGAAT GCAGTTCTTG GCACAAAGAC AAAAGCTGCA 
GTGAAAATTT TGGACTCAAA AGGAGGACAA TGCCATCCTT CATATTCCTC CAACCAAAGC 
AAGCACAGCA CATGGGAGAA GGGCATTTGG CATCTGCTGC CCCCAGGGTC TTCCTCATCC 
ACCACTTCTG GTTCCTTTCA TCTGGAAAGA AGACCTCTTC CATCTTCCAT GCAGCTAGCA 
GTCATCAGGG GAGACACCCT GCGGGGCTTT GATTCTACAG ATCTTTCTCA AAGGAAGCTT 
AGGACCCGTG GGAATGGCAA AACAGTTCGT CCATCCTCTG TTTATAGAAA TGGAACAGAC 
ATCATCTATA ATTATCATGG GATAGTTTCC TTGAAACTGG AGGATGACAG TTTCCCAACT 
CACAAAAGGA AGGCCAAAGT ATCCATCATT AGTCAGCCAC AAAAGACAAT CAAAGTGGCA 
GAACTGCCTC AAGCAGATAA GGTGGAATCC ACAACTGACT CACACTTCCC CAGACAGGAC 
CAGTTGCCCT CATTTCCAAA GAACTGCACT CTGGAATTAA AGGGACTCTT CCATTTTGAA 
GAAGGCATCC AGAAGCTGTA TCAGTGCAAT GGGATCGCCT GGAAAGCCTG GAGTCCCCAA 
ACCAAGGATG TGGAAGACAA ATCCTGTCCA GCCGGGTGGC ACCAGCACTC AGGCTACTGT 
CACATCTTGA TCACAGAGCA GAAAGGCACC TGGAATGCGG CTGCCCAAGC TTGCAGGGAA 
CAATACCTGG GCAACCTTGT AACTGTATTC TCCAGGCAGC ACATGCGGTG GCTCTGGGAC 
ATTGGTGGGA GAAAGTCCTT TTGGATAGGT TTGAACGACC AAGTGCATGC TGGCCACTGG 
GAGTGGATCG GTGGTGAACC TGTTGCCTTC ACCAATGGGA GAAGAGGGCC CTCTCAACGC 
TCCAAGCTTG GAAAGAGCTG TGTTTTGGTT CAAAGACAAG GGAAATGGCA AACAAAAGAC 
TGTAGGAGAG CCAAACCTCA TAATTATGTG TGTTCCAGAA AACTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005251441.1
CDS	503..2650
Translation	MVTQESMLKAALPLFTRFIISNGLRTEHGVFEITLETVDRALPVVTRNKGLRLAQGAVGLLSPDLLQLTDPDTPAENLTFLLVQLPQHGQLYLWGTGLLQHNFTQQDVDSKNVAYRHSGGDSQTDCFTFMATDGTNQGFIVNGRVWEEPVLFTIQVDQLDKTAPRITLLHSPSQVGLLKNGCYGIYITSRVLKASDPDTEDDQIIFKILQGPKHGHLENTTTGEFIHEKFSQKDLNSKTILYIINPSLEVNSDTVEFQIMDPTGNSATPQILELKWSHIEWSQTEYEVCENVGLLPLEIIRRGYSMDSAFVGIKVNQVSAAVGKDFTVIPSKLIQFDPGMSTKMWNIAITYDGLEEDDEVFEVILNSPVNAVLGTKTKAAVKILDSKGGQCHPSYSSNQSKHSTWEKGIWHLLPPGSSSSTTSGSFHLERRPLPSSMQLAVIRGDTLRGFDSTDLSQRKLRTRGNGKTVRPSSVYRNGTDIIYNYHGIVSLKLEDDSFPTHKRKAKVSIISQPQKTIKVAELPQADKVESTTDSHFPRQDQLPSFPKNCTLELKGLFHFEEGIQKLYQCNGIAWKAWSPQTKDVEDKSCPAGWHQHSGYCHILITEQKGTWNAAAQACREQYLGNLVTVFSRQHMRWLWDIGGRKSFWIGLNDQVHAGHWEWIGGEPVAFTNGRRGPSQRSKLGKSCVLVQRQGKWQTKDCRRAKPHNYVCSRKL

Target ORF information:

RefSeq Version	XM_005251384.4
Organism	Homo sapiens(human)
Definition	Homo sapiens FRAS1 related extracellular matrix 1 (FREM1), transcript variant X22, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005251384.4

ORF Insert Sequence:

ATGGTGACAC AAGAATCCAT GCTGAAGGCT GCCTTGCCTC TCTTTACCAG ATTCATCATC 
AGCAATGGAC TGCGGACCGA GCACGGGGTG TTTGAGATCA CACTGGAGAC TGTGGACAGA 
GCCCTGCCTG TGGTAACCAG GAACAAGGGG TTGAGACTGG CCCAAGGGGC CGTGGGCCTG 
CTTTCCCCTG ACCTCCTTCA GCTGACCGAC CCTGATACAC CTGCGGAGAA CCTCACCTTC 
CTCTTGGTTC AGCTCCCCCA GCATGGCCAG CTCTACCTGT GGGGGACAGG GCTACTTCAA 
CACAATTTCA CCCAGCAGGA TGTGGACAGC AAGAATGTGG CCTATCGGCA CTCAGGAGGG 
GACTCCCAGA CTGACTGCTT TACTTTCATG GCCACAGATG GGACAAACCA AGGCTTTATT 
GTGAATGGGA GAGTGTGGGA AGAACCTGTT TTATTCACCA TTCAGGTAGA CCAATTGGAC 
AAAACAGCTC CTCGTATCAC ACTCTTGCAT TCCCCTTCTC AAGTGGGGCT CCTGAAAAAT 
GGCTGCTACG GGATTTACAT CACTTCCCGC GTGTTGAAGG CATCAGACCC TGACACTGAG 
GACGATCAGA TCATCTTTAA AATTCTACAA GGCCCAAAAC ATGGACATCT GGAGAACACA 
ACAACAGGTG AATTTATCCA TGAGAAATTT AGCCAAAAGG ACTTAAACAG TAAGACTATT 
CTTTACATCA TAAACCCATC TTTGGAAGTA AATTCAGATA CCGTGGAATT TCAAATCATG 
GACCCCACAG GGAACTCGGC CACTCCTCAA ATTTTGGAAC TGAAGTGGTC TCATATTGAA 
TGGTCACAGA CCGAATATGA AGTCTGTGAG AATGTGGGTT TGTTGCCCTT GGAAATTATC 
AGAAGGGGAT ATTCCATGGA CTCGGCCTTT GTGGGTATAA AGGTCAACCA AGTGTCAGCT 
GCAGTTGGAA AAGATTTCAC CGTGATTCCA TCTAAACTGA TTCAGTTTGA CCCAGGAATG 
TCAACTAAGA TGTGGAATAT AGCAATTACC TATGACGGAT TAGAGGAAGA TGATGAGGTC 
TTTGAAGTAA TTCTGAACTC CCCTGTGAAT GCAGTTCTTG GCACAAAGAC AAAAGCTGCA 
GTGAAAATTT TGGACTCAAA AGGAGGACAA TGCCATCCTT CATATTCCTC CAACCAAAGC 
AAGCACAGCA CATGGGAGAA GGGCATTTGG CATCTGCTGC CCCCAGGGTC TTCCTCATCC 
ACCACTTCTG GTTCCTTTCA TCTGGAAAGA AGACCTCTTC CATCTTCCAT GCAGCTAGCA 
GTCATCAGGG GAGACACCCT GCGGGGCTTT GATTCTACAG ATCTTTCTCA AAGGAAGCTT 
AGGACCCGTG GGAATGGCAA AACAGTTCGT CCATCCTCTG TTTATAGAAA TGGAACAGAC 
ATCATCTATA ATTATCATGG GATAGTTTCC TTGAAACTGG AGGATGACAG TTTCCCAACT 
CACAAAAGGA AGGCCAAAGT ATCCATCATT AGTCAGCCAC AAAAGACAAT CAAAGTGGCA 
GAACTGCCTC AAGCAGATAA GGTGGAATCC ACAACTGACT CACACTTCCC CAGACAGGAC 
CAGTTGCCCT CATTTCCAAA GAACTGCACT CTGGAATTAA AGGGACTCTT CCATTTTGAA 
GAAGGCATCC AGAAGCTGTA TCAGTGCAAT GGGATCGCCT GGAAAGCCTG GAGTCCCCAA 
ACCAAGGATG TGGAAGACAA ATCCTGTCCA GCCGGGTGGC ACCAGCACTC AGGCTACTGT 
CACATCTTGA TCACAGAGCA GAAAGGCACC TGGAATGCGG CTGCCCAAGC TTGCAGGGAA 
CAATACCTGG GCAACCTTGT AACTGTATTC TCCAGGCAGC ACATGCGGTG GCTCTGGGAC 
ATTGGTGGGA GAAAGTCCTT TTGGATAGGT TTGAACGACC AAGTGCATGC TGGCCACTGG 
GAGTGGATCG GTGGTGAACC TGTTGCCTTC ACCAATGGGA GAAGAGGGCC CTCTCAACGC 
TCCAAGCTTG GAAAGAGCTG TGTTTTGGTT CAAAGACAAG GGAAATGGCA AACAAAAGAC 
TGTAGGAGAG CCAAACCTCA TAATTATGTG TGTTCCAGAA AACTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FREM1 ( XM_011517758.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011516060.1 Homo sapiens FRAS1 related extracellular matrix 1 (FREM1), transcript variant X20, mRNA.

Price & Availability↑

CloneID	OHu76988
Clone ID Related Accession (Same CDS sequence)	XM_011517758.2
Accession Version	XM_011517758.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2184bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	FRAS1-related extracellular matrix protein 1 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011517758.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGTCCC TGGCACTCAC TCAAGCTTGC ATTTGGATGG TGACACAAGA ATCCATGCTG 
AAGGCTGCCT TGCCTCTCTT TACCAGATTC ATCATCAGCA ATGGACTGCG GACCGAGCAC 
GGGGTGTTTG AGATCACACT GGAGACTGTG GACAGAGCCC TGCCTGTGGT AACCAGGAAC 
AAGGGGTTGA GACTGGCCCA AGGGGCCGTG GGCCTGCTTT CCCCTGACCT CCTTCAGCTG 
ACCGACCCTG ATACACCTGC GGAGAACCTC ACCTTCCTCT TGGTTCAGCT CCCCCAGCAT 
GGCCAGCTCT ACCTGTGGGG GACAGGGCTA CTTCAACACA ATTTCACCCA GCAGGATGTG 
GACAGCAAGA ATGTGGCCTA TCGGCACTCA GGAGGGGACT CCCAGACTGA CTGCTTTACT 
TTCATGGCCA CAGATGGGAC AAACCAAGGC TTTATTGTGA ATGGGAGAGT GTGGGAAGAA 
CCTGTTTTAT TCACCATTCA GGTAGACCAA TTGGACAAAA CAGCTCCTCG TATCACACTC 
TTGCATTCCC CTTCTCAAGT GGGGCTCCTG AAAAATGGCT GCTACGGGAT TTACATCACT 
TCCCGCGTGT TGAAGGCATC AGACCCTGAC ACTGAGGACG ATCAGATCAT CTTTAAAATT 
CTACAAGGCC CAAAACATGG ACATCTGGAG AACACAACAA CAGGTGAATT TATCCATGAG 
AAATTTAGCC AAAAGGACTT AAACAGTAAG ACTATTCTTT ACATCATAAA CCCATCTTTG 
GAAGTAAATT CAGATACCGT GGAATTTCAA ATCATGGACC CCACAGGGAA CTCGGCCACT 
CCTCAAATTT TGGAACTGAA GTGGTCTCAT ATTGAATGGT CACAGACCGA ATATGAAGTC 
TGTGAGAATG TGGGTTTGTT GCCCTTGGAA ATTATCAGAA GGGGATATTC CATGGACTCG 
GCCTTTGTGG GTATAAAGGT CAACCAAGTG TCAGCTGCAG TTGGAAAAGA TTTCACCGTG 
ATTCCATCTA AACTGATTCA GTTTGACCCA GGAATGTCAA CTAAGATGTG GAATATAGCA 
ATTACCTATG ACGGATTAGA GGAAGATGAT GAGGTCTTTG AAGTAATTCT GAACTCCCCT 
GTGAATGCAG TTCTTGGCAC AAAGACAAAA GCTGCAGTGA AAATTTTGGA CTCAAAAGGA 
GGACAATGCC ATCCTTCATA TTCCTCCAAC CAAAGCAAGC ACAGCACATG GGAGAAGGGC 
ATTTGGCATC TGCTGCCCCC AGGGTCTTCC TCATCCACCA CTTCTGGTTC CTTTCATCTG 
GAAAGAAGAC CTCTTCCATC TTCCATGCAG CTAGCAGTCA TCAGGGGAGA CACCCTGCGG 
GGCTTTGATT CTACAGATCT TTCTCAAAGG AAGCTTAGGA CCCGTGGGAA TGGCAAAACA 
GTTCGTCCAT CCTCTGTTTA TAGAAATGGA ACAGACATCA TCTATAATTA TCATGGGATA 
GTTTCCTTGA AACTGGAGGA TGACAGTTTC CCAACTCACA AAAGGAAGGC CAAAGTATCC 
ATCATTAGTC AGCCACAAAA GACAATCAAA GTGGCAGAAC TGCCTCAAGC AGATAAGGTG 
GAATCCACAA CTGACTCACA CTTCCCCAGA CAGGACCAGT TGCCCTCATT TCCAAAGAAC 
TGCACTCTGG AATTAAAGGG ACTCTTCCAT TTTGAAGAAG GCATCCAGAA GCTGTATCAG 
TGCAATGGGA TCGCCTGGAA AGCCTGGAGT CCCCAAACCA AGGATGTGGA AGACAAATCC 
TGTCCAGCCG GGTGGCACCA GCACTCAGGC TACTGTCACA TCTTGATCAC AGAGCAGAAA 
GGCACCTGGA ATGCGGCTGC CCAAGCTTGC AGGGAACAAT ACCTGGGCAA CCTTGTAACT 
GTATTCTCCA GGCAGCACAT GCGGTGGCTC TGGGACATTG GTGGGAGAAA GTCCTTTTGG 
ATAGGTTTGA ACGACCAAGT GCATGCTGGC CACTGGGAGT GGATCGGTGG TGAACCTGTT 
GCCTTCACCA ATGGGAGAAG AGGGCCCTCT CAACGCTCCA AGCTTGGAAA GAGCTGTGTT 
TTGGTTCAAA GACAAGGGAA ATGGCAAACA AAAGACTGTA GGAGAGCCAA ACCTCATAAT 
TATGTGTGTT CCAGAAAACT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011516060.1
CDS	103..2286
Translation	MESLALTQACIWMVTQESMLKAALPLFTRFIISNGLRTEHGVFEITLETVDRALPVVTRNKGLRLAQGAVGLLSPDLLQLTDPDTPAENLTFLLVQLPQHGQLYLWGTGLLQHNFTQQDVDSKNVAYRHSGGDSQTDCFTFMATDGTNQGFIVNGRVWEEPVLFTIQVDQLDKTAPRITLLHSPSQVGLLKNGCYGIYITSRVLKASDPDTEDDQIIFKILQGPKHGHLENTTTGEFIHEKFSQKDLNSKTILYIINPSLEVNSDTVEFQIMDPTGNSATPQILELKWSHIEWSQTEYEVCENVGLLPLEIIRRGYSMDSAFVGIKVNQVSAAVGKDFTVIPSKLIQFDPGMSTKMWNIAITYDGLEEDDEVFEVILNSPVNAVLGTKTKAAVKILDSKGGQCHPSYSSNQSKHSTWEKGIWHLLPPGSSSSTTSGSFHLERRPLPSSMQLAVIRGDTLRGFDSTDLSQRKLRTRGNGKTVRPSSVYRNGTDIIYNYHGIVSLKLEDDSFPTHKRKAKVSIISQPQKTIKVAELPQADKVESTTDSHFPRQDQLPSFPKNCTLELKGLFHFEEGIQKLYQCNGIAWKAWSPQTKDVEDKSCPAGWHQHSGYCHILITEQKGTWNAAAQACREQYLGNLVTVFSRQHMRWLWDIGGRKSFWIGLNDQVHAGHWEWIGGEPVAFTNGRRGPSQRSKLGKSCVLVQRQGKWQTKDCRRAKPHNYVCSRKL

Target ORF information:

RefSeq Version	XM_011517758.2
Organism	Homo sapiens(human)
Definition	Homo sapiens FRAS1 related extracellular matrix 1 (FREM1), transcript variant X20, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011517758.2

ORF Insert Sequence:

ATGGAGTCCC TGGCACTCAC TCAAGCTTGC ATTTGGATGG TGACACAAGA ATCCATGCTG 
AAGGCTGCCT TGCCTCTCTT TACCAGATTC ATCATCAGCA ATGGACTGCG GACCGAGCAC 
GGGGTGTTTG AGATCACACT GGAGACTGTG GACAGAGCCC TGCCTGTGGT AACCAGGAAC 
AAGGGGTTGA GACTGGCCCA AGGGGCCGTG GGCCTGCTTT CCCCTGACCT CCTTCAGCTG 
ACCGACCCTG ATACACCTGC GGAGAACCTC ACCTTCCTCT TGGTTCAGCT CCCCCAGCAT 
GGCCAGCTCT ACCTGTGGGG GACAGGGCTA CTTCAACACA ATTTCACCCA GCAGGATGTG 
GACAGCAAGA ATGTGGCCTA TCGGCACTCA GGAGGGGACT CCCAGACTGA CTGCTTTACT 
TTCATGGCCA CAGATGGGAC AAACCAAGGC TTTATTGTGA ATGGGAGAGT GTGGGAAGAA 
CCTGTTTTAT TCACCATTCA GGTAGACCAA TTGGACAAAA CAGCTCCTCG TATCACACTC 
TTGCATTCCC CTTCTCAAGT GGGGCTCCTG AAAAATGGCT GCTACGGGAT TTACATCACT 
TCCCGCGTGT TGAAGGCATC AGACCCTGAC ACTGAGGACG ATCAGATCAT CTTTAAAATT 
CTACAAGGCC CAAAACATGG ACATCTGGAG AACACAACAA CAGGTGAATT TATCCATGAG 
AAATTTAGCC AAAAGGACTT AAACAGTAAG ACTATTCTTT ACATCATAAA CCCATCTTTG 
GAAGTAAATT CAGATACCGT GGAATTTCAA ATCATGGACC CCACAGGGAA CTCGGCCACT 
CCTCAAATTT TGGAACTGAA GTGGTCTCAT ATTGAATGGT CACAGACCGA ATATGAAGTC 
TGTGAGAATG TGGGTTTGTT GCCCTTGGAA ATTATCAGAA GGGGATATTC CATGGACTCG 
GCCTTTGTGG GTATAAAGGT CAACCAAGTG TCAGCTGCAG TTGGAAAAGA TTTCACCGTG 
ATTCCATCTA AACTGATTCA GTTTGACCCA GGAATGTCAA CTAAGATGTG GAATATAGCA 
ATTACCTATG ACGGATTAGA GGAAGATGAT GAGGTCTTTG AAGTAATTCT GAACTCCCCT 
GTGAATGCAG TTCTTGGCAC AAAGACAAAA GCTGCAGTGA AAATTTTGGA CTCAAAAGGA 
GGACAATGCC ATCCTTCATA TTCCTCCAAC CAAAGCAAGC ACAGCACATG GGAGAAGGGC 
ATTTGGCATC TGCTGCCCCC AGGGTCTTCC TCATCCACCA CTTCTGGTTC CTTTCATCTG 
GAAAGAAGAC CTCTTCCATC TTCCATGCAG CTAGCAGTCA TCAGGGGAGA CACCCTGCGG 
GGCTTTGATT CTACAGATCT TTCTCAAAGG AAGCTTAGGA CCCGTGGGAA TGGCAAAACA 
GTTCGTCCAT CCTCTGTTTA TAGAAATGGA ACAGACATCA TCTATAATTA TCATGGGATA 
GTTTCCTTGA AACTGGAGGA TGACAGTTTC CCAACTCACA AAAGGAAGGC CAAAGTATCC 
ATCATTAGTC AGCCACAAAA GACAATCAAA GTGGCAGAAC TGCCTCAAGC AGATAAGGTG 
GAATCCACAA CTGACTCACA CTTCCCCAGA CAGGACCAGT TGCCCTCATT TCCAAAGAAC 
TGCACTCTGG AATTAAAGGG ACTCTTCCAT TTTGAAGAAG GCATCCAGAA GCTGTATCAG 
TGCAATGGGA TCGCCTGGAA AGCCTGGAGT CCCCAAACCA AGGATGTGGA AGACAAATCC 
TGTCCAGCCG GGTGGCACCA GCACTCAGGC TACTGTCACA TCTTGATCAC AGAGCAGAAA 
GGCACCTGGA ATGCGGCTGC CCAAGCTTGC AGGGAACAAT ACCTGGGCAA CCTTGTAACT 
GTATTCTCCA GGCAGCACAT GCGGTGGCTC TGGGACATTG GTGGGAGAAA GTCCTTTTGG 
ATAGGTTTGA ACGACCAAGT GCATGCTGGC CACTGGGAGT GGATCGGTGG TGAACCTGTT 
GCCTTCACCA ATGGGAGAAG AGGGCCCTCT CAACGCTCCA AGCTTGGAAA GAGCTGTGTT 
TTGGTTCAAA GACAAGGGAA ATGGCAAACA AAAGACTGTA GGAGAGCCAA ACCTCATAAT 
TATGTGTGTT CCAGAAAACT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FREM1 ( NM_001370058.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001356987.1 Homo sapiens FRAS1 related extracellular matrix 1 (FREM1), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu108041
Clone ID Related Accession (Same CDS sequence)	NM_001370058.1
Accession Version	NM_001370058.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1731bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Homo sapiens(human)
Product	FRAS1-related extracellular matrix protein 1 isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL512643.9 and AL390732.10. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.279113.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1966682, SAMEA1968189 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: full length.

ATGGTGACAC AAGAATCCAT GCTGAAGGCT GCCTTGCCTC TCTTTACCAG ATTCATCATC 
AGCAATGGAC TGCGGACCGA GCACGGGGTG TTTGAGATCA CACTGGAGAC TGTGGACAGA 
GCCCTGCCTG TGGTAACCAG GAACAAGGGG TTGAGACTGG CCCAAGGGGC CGTGGGCCTG 
CTTTCCCCTG ACCTCCTTCA GCTGACCGAC CCTGATACAC CTGCGGAGAA CCTCACCTTC 
CTCTTGGTTC AGCTCCCCCA GCATGGCCAG CTCTACCTGT GGGGGACAGG GCTACTTCAA 
CACAATTTCA CCCAGCAGGA TGTGGACAGC AAGAATGTGG CCTATCGGCA CTCAGGAGGG 
GACTCCCAGA CTGACTGCTT TACTTTCATG GCCACAGATG GGACAAACCA AGGCTTTATT 
GTGAATGGGA GAGTGTGGGA AGAACCTGTT TTATTCACCA TTCAGGTAGA CCAATTGGAC 
AAAACAGCTC CTCGTATCAC ACTCTTGCAT TCCCCTTCTC AAGTGGGGCT CCTGAAAAAT 
GGCTGCTACG GGATTTACAT CACTTCCCGC GTGTTGAAGG CATCAGACCC TGACACTGAG 
GACGATCAGA TCATCTTTAA AATTCTACAA GGCCCAAAAC ATGGACATCT GGAGAACACA 
ACAACAGGTG AATTTATCCA TGAGAAATTT AGCCAAAAGG ACTTAAACAG TAAGACTATT 
CTTTACATCA TAAACCCATC TTTGGAAGTA AATTCAGATA CCGTGGAATT TCAAATCATG 
GACCCCACAG GGAACTCGGC CACTCCTCAA ATTTTGGAAC TGAAGTGGTC TCATATTGAA 
TGGTCACAGA CCGAATATGA AGTCTGTGAG AATGTGGGTT TGTTGCCCTT GGAAATTATC 
AGAAGGGGAT ATTCCATGGA CTCGGCCTTT GTGGGTATAA AGGTCAACCA AGTGTCAGCT 
GCAGTTGGAA AAGATTTCAC CGTGATTCCA TCTAAACTGA TTCAGTTTGA CCCAGGACAA 
TGCCATCCTT CATATTCCTC CAACCAAAGC AAGCACAGCA CATGGGAGAA GGGCATTTGG 
CATCTGCTGC CCCCAGGGTC TTCCTCATCC ACCACTTCTG GTTCCTTTCA TCTGGAAAGA 
AGACCTCTTC CATCTTCCAT GCAGCTAGCA GTCATCAGGG GAGACACCCT GCGGGGCTTT 
GATTCTACAG ATCTTTCTCA AAGGAAGCTT AGGACCCGTG GGAATGGCAA AACAGTTCGT 
CCATCCTCTG TTTATAGAAA TGGAACAGAC ATCATCTATA ATTATCATGG GATAGTTTCC 
TTGAAACTGG AGGATGACAG TTTCCCAACT CACAAAAGGA AGGCCAAAGT ATCCATCATT 
AGTCAGCCAC AAAAGACAAT CAAAGTGGCA GAACTGCCTC AAGCAGATAA GGTGGAATCC 
ACAACTGACT CACACTTCCC CAGACAGGAC CAGTTGCCCT CATTTCCAAA GAACTGCACT 
CTGGAATTAA AGGGACTCTT CCATTTTGAA GAAGGCATCC AGAAGCTGTA TCAGTGCAAT 
GGGATCGCCT GGAAAGCCTG GAGTCCCCAA ACCAAGATAC CTGGGCAACC TTGTAACTGT 
ATTCTCCAGG CAGCACATGC GGTGGCTCTG GGACATTGGT GGGAGAAAGT CCTTTTGGAT 
AGGTTTGAAC GACCAAGTGC ATGCTGGCCA CTGGGAGTGG ATCGGTGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001356987.1
CDS	988..2718
Translation	MVTQESMLKAALPLFTRFIISNGLRTEHGVFEITLETVDRALPVVTRNKGLRLAQGAVGLLSPDLLQLTDPDTPAENLTFLLVQLPQHGQLYLWGTGLLQHNFTQQDVDSKNVAYRHSGGDSQTDCFTFMATDGTNQGFIVNGRVWEEPVLFTIQVDQLDKTAPRITLLHSPSQVGLLKNGCYGIYITSRVLKASDPDTEDDQIIFKILQGPKHGHLENTTTGEFIHEKFSQKDLNSKTILYIINPSLEVNSDTVEFQIMDPTGNSATPQILELKWSHIEWSQTEYEVCENVGLLPLEIIRRGYSMDSAFVGIKVNQVSAAVGKDFTVIPSKLIQFDPGQCHPSYSSNQSKHSTWEKGIWHLLPPGSSSSTTSGSFHLERRPLPSSMQLAVIRGDTLRGFDSTDLSQRKLRTRGNGKTVRPSSVYRNGTDIIYNYHGIVSLKLEDDSFPTHKRKAKVSIISQPQKTIKVAELPQADKVESTTDSHFPRQDQLPSFPKNCTLELKGLFHFEEGIQKLYQCNGIAWKAWSPQTKIPGQPCNCILQAAHAVALGHWWEKVLLDRFERPSACWPLGVDRW

Target ORF information:

RefSeq Version	NM_001370058.1
Organism	Homo sapiens(human)
Definition	Homo sapiens FRAS1 related extracellular matrix 1 (FREM1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001370058.1

ORF Insert Sequence:

ATGGTGACAC AAGAATCCAT GCTGAAGGCT GCCTTGCCTC TCTTTACCAG ATTCATCATC 
AGCAATGGAC TGCGGACCGA GCACGGGGTG TTTGAGATCA CACTGGAGAC TGTGGACAGA 
GCCCTGCCTG TGGTAACCAG GAACAAGGGG TTGAGACTGG CCCAAGGGGC CGTGGGCCTG 
CTTTCCCCTG ACCTCCTTCA GCTGACCGAC CCTGATACAC CTGCGGAGAA CCTCACCTTC 
CTCTTGGTTC AGCTCCCCCA GCATGGCCAG CTCTACCTGT GGGGGACAGG GCTACTTCAA 
CACAATTTCA CCCAGCAGGA TGTGGACAGC AAGAATGTGG CCTATCGGCA CTCAGGAGGG 
GACTCCCAGA CTGACTGCTT TACTTTCATG GCCACAGATG GGACAAACCA AGGCTTTATT 
GTGAATGGGA GAGTGTGGGA AGAACCTGTT TTATTCACCA TTCAGGTAGA CCAATTGGAC 
AAAACAGCTC CTCGTATCAC ACTCTTGCAT TCCCCTTCTC AAGTGGGGCT CCTGAAAAAT 
GGCTGCTACG GGATTTACAT CACTTCCCGC GTGTTGAAGG CATCAGACCC TGACACTGAG 
GACGATCAGA TCATCTTTAA AATTCTACAA GGCCCAAAAC ATGGACATCT GGAGAACACA 
ACAACAGGTG AATTTATCCA TGAGAAATTT AGCCAAAAGG ACTTAAACAG TAAGACTATT 
CTTTACATCA TAAACCCATC TTTGGAAGTA AATTCAGATA CCGTGGAATT TCAAATCATG 
GACCCCACAG GGAACTCGGC CACTCCTCAA ATTTTGGAAC TGAAGTGGTC TCATATTGAA 
TGGTCACAGA CCGAATATGA AGTCTGTGAG AATGTGGGTT TGTTGCCCTT GGAAATTATC 
AGAAGGGGAT ATTCCATGGA CTCGGCCTTT GTGGGTATAA AGGTCAACCA AGTGTCAGCT 
GCAGTTGGAA AAGATTTCAC CGTGATTCCA TCTAAACTGA TTCAGTTTGA CCCAGGACAA 
TGCCATCCTT CATATTCCTC CAACCAAAGC AAGCACAGCA CATGGGAGAA GGGCATTTGG 
CATCTGCTGC CCCCAGGGTC TTCCTCATCC ACCACTTCTG GTTCCTTTCA TCTGGAAAGA 
AGACCTCTTC CATCTTCCAT GCAGCTAGCA GTCATCAGGG GAGACACCCT GCGGGGCTTT 
GATTCTACAG ATCTTTCTCA AAGGAAGCTT AGGACCCGTG GGAATGGCAA AACAGTTCGT 
CCATCCTCTG TTTATAGAAA TGGAACAGAC ATCATCTATA ATTATCATGG GATAGTTTCC 
TTGAAACTGG AGGATGACAG TTTCCCAACT CACAAAAGGA AGGCCAAAGT ATCCATCATT 
AGTCAGCCAC AAAAGACAAT CAAAGTGGCA GAACTGCCTC AAGCAGATAA GGTGGAATCC 
ACAACTGACT CACACTTCCC CAGACAGGAC CAGTTGCCCT CATTTCCAAA GAACTGCACT 
CTGGAATTAA AGGGACTCTT CCATTTTGAA GAAGGCATCC AGAAGCTGTA TCAGTGCAAT 
GGGATCGCCT GGAAAGCCTG GAGTCCCCAA ACCAAGATAC CTGGGCAACC TTGTAACTGT 
ATTCTCCAGG CAGCACATGC GGTGGCTCTG GGACATTGGT GGGAGAAAGT CCTTTTGGAT 
AGGTTTGAAC GACCAAGTGC ATGCTGGCCA CTGGGAGTGG ATCGGTGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FREM1 ( XM_017014330.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016869819.1 Homo sapiens FRAS1 related extracellular matrix 1 (FREM1), transcript variant X19, mRNA.

Price & Availability↑

CloneID	OHu98466
Clone ID Related Accession (Same CDS sequence)	XM_017014330.2 , XM_017014330.1
Accession Version	XM_017014330.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4014bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	FRAS1-related extracellular matrix protein 1 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017014330.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACTCTC TGAGTTGGGG GGCTGCGAAT GCCGTGCTGC TGCTGCTCCT CCTGGCCTGG 
GCCAGCCCCA CCTTCATCAG CATCAACCGC GGGGTGAGGG TGATGAAGGG CCACTCTGCC 
TTCCTGTCAG GAGATGACCT GAAGTTTGCC ATCCCTAAAG AGAAAGATGC CTGCAAAGTG 
GAAGTTGTGA TGAATGAGCC AATAACCCAG AGGGTTGGGA AACTCACTCC ACAGGTCTTT 
GACTGCCATT TCCTTCCCAA CGAAGTCAAG TATGTTCACA ATGGTTGTCC AATTCTTGAT 
GAAGACACAG TGAAGCTCAG ACTTTACAGA TTTACTGAAA GAGATACCTT CATAGAAACT 
TTTATCCTGT GGGTCTATCT CCTGGAACCA GACTGTAACA TCATCCATAT GAGTAACAAT 
GTGCTGGAGG TGCCTGAATT CAATGGCTTG TCCCAAGCGA TTGATAAAAA TCTGCTCAGA 
TTCGATTATG ATAGGATGGC TAGCCTGGAA TGTACCGTCA GCCTGGACAC TGCGAGAACT 
CGGCTGCCAG CCCATGGCCA GATGGTTCTC GGGGAGCCTC GACCAGAAGA ACCTCGTGGA 
GATCAGCCAC ACAGTTTTTT CCCAGAGTCC CGTATGATGT CTTTTAATGC CTTTCTGGAA 
CTGAGAGCAA AACTGAAGTG TCCAGGTGGG AGCTGTACCC CAGGATTAAA GAAAATAGGA 
AGCCTCAAAG TGAGCTGTGA GGAGTTCCTG CTGATGGGCC TTCGTTATCA GCATCTGGAT 
CCCCCTTCAC CCAACATTGA TTATATCTCC ATCCAACTGG ACCTCACAGA TACCAGGAGC 
AAAATTGTGT ACAAGTCAGA GAGTGCGTGG CTGCCTGTCT ATATCAGAGC TGGAATTCCG 
AATCAGATTC CAAAGGCTGC ATTCATGGCC GTGTTTATTC TGGAAGTGGA TCAGTTCATC 
CTGACCTCCT TGACTACATC AGTTCTGGAC TGTGAAGAAG ATGAGACCCC TAAACCCTTG 
CTGGTGTTCA ACATTACTAA AGCCCCGCTC CAGGGCTATG TGACTCACCT GTTGGATCAC 
ACCAGACCAA TCTCCTCATT CACCTGGAAA GATCTCAGTG ACATGCAGAT CGCCTATCAG 
CCACCAAACA GCAGCCATTC TGAGAGGAGA CATGATGAGG TAGAATTGGA GGTATACGAC 
TTCTTCTTTG AAAGGAGTGC ACCTATGACA GTCCACATCT CCATCAGAAC AGCAGATACA 
AATGCCCCCC GTGTATCCTG GAATACAGGT CTGAGTCTCC TTGAGGGGCA GTCTCGAGCC 
ATCACTTGGG AACAGTTTCA GGTTGTCGAC AATGACGACA TTGGTGCTGT CCGGCTAGTC 
ACCGTTGGTG GCCTGCAGCA TGGATGGCTG ACTTTAAGAG GGGGGAAAGG GTTTCTCTTC 
ACCGTGGCTG ACCTCCAGGC TGGAGTTGTT CGCTATCATC ATGATGACAG CGACTCCACC 
AAAGACTTCG TGGTCTTCCG GATATTTGAT GGCCATCACA GCATCCGTCA CAAATTCCCC 
ATCAACGTCT TGCCCAAAGA TGATAGTCCC CCGTTCCTCA TAACCAATGT TGTGATTGAA 
CTGGAGGAGG GGCAGACCAT CCTGATCCAG GGATCCATGC TGCGAGCTTC AGATGTGGAC 
GCCAGTGATG ACTACATCTT CTTCAATATC ACAAAGCCTC CACAGGCTGG GGAGATCATG 
AAGAAGCCAG GGCCAGGACT GATAGGCTAT CCTGTCCATG GCTTCCTTCA GAGGGATTTG 
TTTAATGGAA TCATTTATTA TCGTCATTTT GGTGGAGAAA TCTTTGAAGA TTCTTTTCAA 
TTTGTCCTGT GGGACAGCCA TGAACCTCCA AATCTTTCAG TGCCACAGGT GGCAACAATC 
CATATAACTC CAGTGGATGA CCAGCTTCCA AAAGAGGCTC CTGGAGTTTC TCGGCATTTG 
GTTGTCAAGG AAACTGAGGT GGCCTATATA ACTAAGAAAC AGCTACATTT TATAGATTCA 
GAATCATATG ACAGGGAGCT GGTCTACACA ATAACTACTC CTCCATTTTT CTCCTTCAGC 
CACAGACACT TGGATGCTGG GAAATTATTT ATGGTGGACA GCATACCAAA AGTAGTTAAG 
AATCCTACGG CCCTGGAGCT GAGGTCATTC ACTCAGCATG CTGTGAACTA TATGAAAGTG 
GCCTACATGC CCCCCATGCA AGACATTGGT CCCCATTGCA GAGATGTCCA GTTCACATTT 
TCTGTCAGTA ACCAACATGG CGGTACTTTG CATGGGATCT GCTTTAACAT TACAATCCTC 
CCAGTGGACA ATCAAGTTCC TGAGGCGTTC ACCAACCCTC TGAAAGTGAC TGAGGGAGGT 
CAAAGCATCA TCAGCACAGA GCACATTCTA ATTTCTGATG CAGATACCAA GCTGGACAAT 
ATTGACCTCT CCCTGCGGGA ATTGCCTCTG CACGGAAGGG TGGAGCTGAA TGGATTTCCT 
CTAAATTCAG GGGGCACATT TTCTTGGGGC GATCTCCATA CCTTAAAAGT TAGGTATCAA 
CATGATGGAA CTGAAGTTCT TCAGGATGAC CTACTCTTGG AGGTCACCGA TGGCACAAAT 
TCAGCAGAAT TTGTACTACA TGTTGAGGTA TTCCCTGTCA ACGATGAGCC ACCAGTCTTA 
AAGGCTGACC TCATGCCTGT CATGAATTGC TCAGAGGGAG GAGAGGTGGT CATCACCTCT 
GAATACATTT TTGCTACTGA TGTGGACAGC GATAACTTGA AGTTGATGTT TGTGATTGCT 
CGCGAACCTC AGCATGGGGT GGTGAGGAGA GCTGGAGTCA CAGTGGATCA GTTCTCTCAG 
AGAGATGTTA TCTCAGAGGC CGTGACATAC AAACACACAG GTGGCGAGAT TGGCCTGATG 
CCTTGTTTTG ACACCATTAC ATTGGTGGTT TCGGATGGAG AGGCTGGCCC TTTTGTGAAT 
GGCTGTTGCT ACAATGGACC CAATCCATCT GTTCCACTTC ATGCGTCCTT TCCAGTGTAT 
GATCTCAACA TCACGGTATA CCCAGTAGAC AACCAGCCAC CTTCCATTGC AATAGGTCCC 
GTGTTTGTTG TAGATGAGGG CTGCTCAACA GCCCTTACTG TTAACCATTT GTCCGCCACT 
GACCCTGACA CTGCAGCAGA TGACTTGGAA TTTGTTTTGG TTTCTCCTCC TCAGTTTGGC 
TACCTCGAAA ATATACTCCC TTCTGTGGGT TTTGAAAAAA GCAATATTGG CATAAGTATA 
GATTCATTTC AGTGGAAAGA CATGAACGCT TTTCACATTA ACTATGTGCA GTCCAGGCAT 
CTGAGGATAG AACCAACTGC CGACCAGTTC ACGGTGTACG TCACAGATGG GAAGCATCAC 
TCCTTGGAGA TACCATTTTC TATTATAATC AACCCCACAA ATGATGAAGC TCCTGACTTT 
GTAGTGCAGA ATATTACTGT GTGTGAGGGT CAGATGAAAG AGCTGGACTC TTCCATCATC 
AGCGCTGTGG ACCTGGACAT TCCCCAGGAT GCCCTGCTGT TCAGCATCAC TCAAAAGCCA 
CGCCATGGCC TCCTCATCGA TAGGGGGTTT AGCAAAGACT TCTCTGAGAA TAAGCAGCCA 
GCCAACCCTC ACCAGAAACA TGCACCTGTT CACAGCTTTT CCATGGAACT CCTCAAGACT 
GGAATGAGGT TGACGTACAT GCATGATGAC TCAGAGAGCC TTGCTGATGA TTTTACAATC 
CAATTGTCAG ATGGGAAACA TAAGATACTT AAAACCATTT CAGTAGAGGT CATCCCAGTT 
AATGATGAAA AACCAATGCT GAGCAAGAAG GCTGAAATTG CAATGAATAT GGGTGAAACT 
CGTATTATTT CCAGTGCTAT TCTTTCAGCC ATAGATGAAG ACTCACCCAG GGAGAAGATT 
TACTATGTAT TTGAAAGGCT TCCCCAAAAT GGGCAACTTC AGCTTAAGGC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016869819.1
CDS	787..4800
Translation	MNSLSWGAANAVLLLLLLAWASPTFISINRGVRVMKGHSAFLSGDDLKFAIPKEKDACKVEVVMNEPITQRVGKLTPQVFDCHFLPNEVKYVHNGCPILDEDTVKLRLYRFTERDTFIETFILWVYLLEPDCNIIHMSNNVLEVPEFNGLSQAIDKNLLRFDYDRMASLECTVSLDTARTRLPAHGQMVLGEPRPEEPRGDQPHSFFPESRMMSFNAFLELRAKLKCPGGSCTPGLKKIGSLKVSCEEFLLMGLRYQHLDPPSPNIDYISIQLDLTDTRSKIVYKSESAWLPVYIRAGIPNQIPKAAFMAVFILEVDQFILTSLTTSVLDCEEDETPKPLLVFNITKAPLQGYVTHLLDHTRPISSFTWKDLSDMQIAYQPPNSSHSERRHDEVELEVYDFFFERSAPMTVHISIRTADTNAPRVSWNTGLSLLEGQSRAITWEQFQVVDNDDIGAVRLVTVGGLQHGWLTLRGGKGFLFTVADLQAGVVRYHHDDSDSTKDFVVFRIFDGHHSIRHKFPINVLPKDDSPPFLITNVVIELEEGQTILIQGSMLRASDVDASDDYIFFNITKPPQAGEIMKKPGPGLIGYPVHGFLQRDLFNGIIYYRHFGGEIFEDSFQFVLWDSHEPPNLSVPQVATIHITPVDDQLPKEAPGVSRHLVVKETEVAYITKKQLHFIDSESYDRELVYTITTPPFFSFSHRHLDAGKLFMVDSIPKVVKNPTALELRSFTQHAVNYMKVAYMPPMQDIGPHCRDVQFTFSVSNQHGGTLHGICFNITILPVDNQVPEAFTNPLKVTEGGQSIISTEHILISDADTKLDNIDLSLRELPLHGRVELNGFPLNSGGTFSWGDLHTLKVRYQHDGTEVLQDDLLLEVTDGTNSAEFVLHVEVFPVNDEPPVLKADLMPVMNCSEGGEVVITSEYIFATDVDSDNLKLMFVIAREPQHGVVRRAGVTVDQFSQRDVISEAVTYKHTGGEIGLMPCFDTITLVVSDGEAGPFVNGCCYNGPNPSVPLHASFPVYDLNITVYPVDNQPPSIAIGPVFVVDEGCSTALTVNHLSATDPDTAADDLEFVLVSPPQFGYLENILPSVGFEKSNIGISIDSFQWKDMNAFHINYVQSRHLRIEPTADQFTVYVTDGKHHSLEIPFSIIINPTNDEAPDFVVQNITVCEGQMKELDSSIISAVDLDIPQDALLFSITQKPRHGLLIDRGFSKDFSENKQPANPHQKHAPVHSFSMELLKTGMRLTYMHDDSESLADDFTIQLSDGKHKILKTISVEVIPVNDEKPMLSKKAEIAMNMGETRIISSAILSAIDEDSPREKIYYVFERLPQNGQLQLKA

Target ORF information:

RefSeq Version	XM_017014330.2
Organism	Homo sapiens(human)
Definition	Homo sapiens FRAS1 related extracellular matrix 1 (FREM1), transcript variant X19, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017014330.2

ORF Insert Sequence:

ATGAACTCTC TGAGTTGGGG GGCTGCGAAT GCCGTGCTGC TGCTGCTCCT CCTGGCCTGG 
GCCAGCCCCA CCTTCATCAG CATCAACCGC GGGGTGAGGG TGATGAAGGG CCACTCTGCC 
TTCCTGTCAG GAGATGACCT GAAGTTTGCC ATCCCTAAAG AGAAAGATGC CTGCAAAGTG 
GAAGTTGTGA TGAATGAGCC AATAACCCAG AGGGTTGGGA AACTCACTCC ACAGGTCTTT 
GACTGCCATT TCCTTCCCAA CGAAGTCAAG TATGTTCACA ATGGTTGTCC AATTCTTGAT 
GAAGACACAG TGAAGCTCAG ACTTTACAGA TTTACTGAAA GAGATACCTT CATAGAAACT 
TTTATCCTGT GGGTCTATCT CCTGGAACCA GACTGTAACA TCATCCATAT GAGTAACAAT 
GTGCTGGAGG TGCCTGAATT CAATGGCTTG TCCCAAGCGA TTGATAAAAA TCTGCTCAGA 
TTCGATTATG ATAGGATGGC TAGCCTGGAA TGTACCGTCA GCCTGGACAC TGCGAGAACT 
CGGCTGCCAG CCCATGGCCA GATGGTTCTC GGGGAGCCTC GACCAGAAGA ACCTCGTGGA 
GATCAGCCAC ACAGTTTTTT CCCAGAGTCC CGTATGATGT CTTTTAATGC CTTTCTGGAA 
CTGAGAGCAA AACTGAAGTG TCCAGGTGGG AGCTGTACCC CAGGATTAAA GAAAATAGGA 
AGCCTCAAAG TGAGCTGTGA GGAGTTCCTG CTGATGGGCC TTCGTTATCA GCATCTGGAT 
CCCCCTTCAC CCAACATTGA TTATATCTCC ATCCAACTGG ACCTCACAGA TACCAGGAGC 
AAAATTGTGT ACAAGTCAGA GAGTGCGTGG CTGCCTGTCT ATATCAGAGC TGGAATTCCG 
AATCAGATTC CAAAGGCTGC ATTCATGGCC GTGTTTATTC TGGAAGTGGA TCAGTTCATC 
CTGACCTCCT TGACTACATC AGTTCTGGAC TGTGAAGAAG ATGAGACCCC TAAACCCTTG 
CTGGTGTTCA ACATTACTAA AGCCCCGCTC CAGGGCTATG TGACTCACCT GTTGGATCAC 
ACCAGACCAA TCTCCTCATT CACCTGGAAA GATCTCAGTG ACATGCAGAT CGCCTATCAG 
CCACCAAACA GCAGCCATTC TGAGAGGAGA CATGATGAGG TAGAATTGGA GGTATACGAC 
TTCTTCTTTG AAAGGAGTGC ACCTATGACA GTCCACATCT CCATCAGAAC AGCAGATACA 
AATGCCCCCC GTGTATCCTG GAATACAGGT CTGAGTCTCC TTGAGGGGCA GTCTCGAGCC 
ATCACTTGGG AACAGTTTCA GGTTGTCGAC AATGACGACA TTGGTGCTGT CCGGCTAGTC 
ACCGTTGGTG GCCTGCAGCA TGGATGGCTG ACTTTAAGAG GGGGGAAAGG GTTTCTCTTC 
ACCGTGGCTG ACCTCCAGGC TGGAGTTGTT CGCTATCATC ATGATGACAG CGACTCCACC 
AAAGACTTCG TGGTCTTCCG GATATTTGAT GGCCATCACA GCATCCGTCA CAAATTCCCC 
ATCAACGTCT TGCCCAAAGA TGATAGTCCC CCGTTCCTCA TAACCAATGT TGTGATTGAA 
CTGGAGGAGG GGCAGACCAT CCTGATCCAG GGATCCATGC TGCGAGCTTC AGATGTGGAC 
GCCAGTGATG ACTACATCTT CTTCAATATC ACAAAGCCTC CACAGGCTGG GGAGATCATG 
AAGAAGCCAG GGCCAGGACT GATAGGCTAT CCTGTCCATG GCTTCCTTCA GAGGGATTTG 
TTTAATGGAA TCATTTATTA TCGTCATTTT GGTGGAGAAA TCTTTGAAGA TTCTTTTCAA 
TTTGTCCTGT GGGACAGCCA TGAACCTCCA AATCTTTCAG TGCCACAGGT GGCAACAATC 
CATATAACTC CAGTGGATGA CCAGCTTCCA AAAGAGGCTC CTGGAGTTTC TCGGCATTTG 
GTTGTCAAGG AAACTGAGGT GGCCTATATA ACTAAGAAAC AGCTACATTT TATAGATTCA 
GAATCATATG ACAGGGAGCT GGTCTACACA ATAACTACTC CTCCATTTTT CTCCTTCAGC 
CACAGACACT TGGATGCTGG GAAATTATTT ATGGTGGACA GCATACCAAA AGTAGTTAAG 
AATCCTACGG CCCTGGAGCT GAGGTCATTC ACTCAGCATG CTGTGAACTA TATGAAAGTG 
GCCTACATGC CCCCCATGCA AGACATTGGT CCCCATTGCA GAGATGTCCA GTTCACATTT 
TCTGTCAGTA ACCAACATGG CGGTACTTTG CATGGGATCT GCTTTAACAT TACAATCCTC 
CCAGTGGACA ATCAAGTTCC TGAGGCGTTC ACCAACCCTC TGAAAGTGAC TGAGGGAGGT 
CAAAGCATCA TCAGCACAGA GCACATTCTA ATTTCTGATG CAGATACCAA GCTGGACAAT 
ATTGACCTCT CCCTGCGGGA ATTGCCTCTG CACGGAAGGG TGGAGCTGAA TGGATTTCCT 
CTAAATTCAG GGGGCACATT TTCTTGGGGC GATCTCCATA CCTTAAAAGT TAGGTATCAA 
CATGATGGAA CTGAAGTTCT TCAGGATGAC CTACTCTTGG AGGTCACCGA TGGCACAAAT 
TCAGCAGAAT TTGTACTACA TGTTGAGGTA TTCCCTGTCA ACGATGAGCC ACCAGTCTTA 
AAGGCTGACC TCATGCCTGT CATGAATTGC TCAGAGGGAG GAGAGGTGGT CATCACCTCT 
GAATACATTT TTGCTACTGA TGTGGACAGC GATAACTTGA AGTTGATGTT TGTGATTGCT 
CGCGAACCTC AGCATGGGGT GGTGAGGAGA GCTGGAGTCA CAGTGGATCA GTTCTCTCAG 
AGAGATGTTA TCTCAGAGGC CGTGACATAC AAACACACAG GTGGCGAGAT TGGCCTGATG 
CCTTGTTTTG ACACCATTAC ATTGGTGGTT TCGGATGGAG AGGCTGGCCC TTTTGTGAAT 
GGCTGTTGCT ACAATGGACC CAATCCATCT GTTCCACTTC ATGCGTCCTT TCCAGTGTAT 
GATCTCAACA TCACGGTATA CCCAGTAGAC AACCAGCCAC CTTCCATTGC AATAGGTCCC 
GTGTTTGTTG TAGATGAGGG CTGCTCAACA GCCCTTACTG TTAACCATTT GTCCGCCACT 
GACCCTGACA CTGCAGCAGA TGACTTGGAA TTTGTTTTGG TTTCTCCTCC TCAGTTTGGC 
TACCTCGAAA ATATACTCCC TTCTGTGGGT TTTGAAAAAA GCAATATTGG CATAAGTATA 
GATTCATTTC AGTGGAAAGA CATGAACGCT TTTCACATTA ACTATGTGCA GTCCAGGCAT 
CTGAGGATAG AACCAACTGC CGACCAGTTC ACGGTGTACG TCACAGATGG GAAGCATCAC 
TCCTTGGAGA TACCATTTTC TATTATAATC AACCCCACAA ATGATGAAGC TCCTGACTTT 
GTAGTGCAGA ATATTACTGT GTGTGAGGGT CAGATGAAAG AGCTGGACTC TTCCATCATC 
AGCGCTGTGG ACCTGGACAT TCCCCAGGAT GCCCTGCTGT TCAGCATCAC TCAAAAGCCA 
CGCCATGGCC TCCTCATCGA TAGGGGGTTT AGCAAAGACT TCTCTGAGAA TAAGCAGCCA 
GCCAACCCTC ACCAGAAACA TGCACCTGTT CACAGCTTTT CCATGGAACT CCTCAAGACT 
GGAATGAGGT TGACGTACAT GCATGATGAC TCAGAGAGCC TTGCTGATGA TTTTACAATC 
CAATTGTCAG ATGGGAAACA TAAGATACTT AAAACCATTT CAGTAGAGGT CATCCCAGTT 
AATGATGAAA AACCAATGCT GAGCAAGAAG GCTGAAATTG CAATGAATAT GGGTGAAACT 
CGTATTATTT CCAGTGCTAT TCTTTCAGCC ATAGATGAAG ACTCACCCAG GGAGAAGATT 
TACTATGTAT TTGAAAGGCT TCCCCAAAAT GGGCAACTTC AGCTTAAGGC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FREM1 ( XM_017014330.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016869819.1 Homo sapiens FRAS1 related extracellular matrix 1 (FREM1), transcript variant X20, mRNA.

Price & Availability↑

CloneID	OHu98466
Clone ID Related Accession (Same CDS sequence)	XM_017014330.2 , XM_017014330.1
Accession Version	XM_017014330.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4014bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	FRAS1-related extracellular matrix protein 1 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACTCTC TGAGTTGGGG GGCTGCGAAT GCCGTGCTGC TGCTGCTCCT CCTGGCCTGG 
GCCAGCCCCA CCTTCATCAG CATCAACCGC GGGGTGAGGG TGATGAAGGG CCACTCTGCC 
TTCCTGTCAG GAGATGACCT GAAGTTTGCC ATCCCTAAAG AGAAAGATGC CTGCAAAGTG 
GAAGTTGTGA TGAATGAGCC AATAACCCAG AGGGTTGGGA AACTCACTCC ACAGGTCTTT 
GACTGCCATT TCCTTCCCAA CGAAGTCAAG TATGTTCACA ATGGTTGTCC AATTCTTGAT 
GAAGACACAG TGAAGCTCAG ACTTTACAGA TTTACTGAAA GAGATACCTT CATAGAAACT 
TTTATCCTGT GGGTCTATCT CCTGGAACCA GACTGTAACA TCATCCATAT GAGTAACAAT 
GTGCTGGAGG TGCCTGAATT CAATGGCTTG TCCCAAGCGA TTGATAAAAA TCTGCTCAGA 
TTCGATTATG ATAGGATGGC TAGCCTGGAA TGTACCGTCA GCCTGGACAC TGCGAGAACT 
CGGCTGCCAG CCCATGGCCA GATGGTTCTC GGGGAGCCTC GACCAGAAGA ACCTCGTGGA 
GATCAGCCAC ACAGTTTTTT CCCAGAGTCC CGTATGATGT CTTTTAATGC CTTTCTGGAA 
CTGAGAGCAA AACTGAAGTG TCCAGGTGGG AGCTGTACCC CAGGATTAAA GAAAATAGGA 
AGCCTCAAAG TGAGCTGTGA GGAGTTCCTG CTGATGGGCC TTCGTTATCA GCATCTGGAT 
CCCCCTTCAC CCAACATTGA TTATATCTCC ATCCAACTGG ACCTCACAGA TACCAGGAGC 
AAAATTGTGT ACAAGTCAGA GAGTGCGTGG CTGCCTGTCT ATATCAGAGC TGGAATTCCG 
AATCAGATTC CAAAGGCTGC ATTCATGGCC GTGTTTATTC TGGAAGTGGA TCAGTTCATC 
CTGACCTCCT TGACTACATC AGTTCTGGAC TGTGAAGAAG ATGAGACCCC TAAACCCTTG 
CTGGTGTTCA ACATTACTAA AGCCCCGCTC CAGGGCTATG TGACTCACCT GTTGGATCAC 
ACCAGACCAA TCTCCTCATT CACCTGGAAA GATCTCAGTG ACATGCAGAT CGCCTATCAG 
CCACCAAACA GCAGCCATTC TGAGAGGAGA CATGATGAGG TAGAATTGGA GGTATACGAC 
TTCTTCTTTG AAAGGAGTGC ACCTATGACA GTCCACATCT CCATCAGAAC AGCAGATACA 
AATGCCCCCC GTGTATCCTG GAATACAGGT CTGAGTCTCC TTGAGGGGCA GTCTCGAGCC 
ATCACTTGGG AACAGTTTCA GGTTGTCGAC AATGACGACA TTGGTGCTGT CCGGCTAGTC 
ACCGTTGGTG GCCTGCAGCA TGGATGGCTG ACTTTAAGAG GGGGGAAAGG GTTTCTCTTC 
ACCGTGGCTG ACCTCCAGGC TGGAGTTGTT CGCTATCATC ATGATGACAG CGACTCCACC 
AAAGACTTCG TGGTCTTCCG GATATTTGAT GGCCATCACA GCATCCGTCA CAAATTCCCC 
ATCAACGTCT TGCCCAAAGA TGATAGTCCC CCGTTCCTCA TAACCAATGT TGTGATTGAA 
CTGGAGGAGG GGCAGACCAT CCTGATCCAG GGATCCATGC TGCGAGCTTC AGATGTGGAC 
GCCAGTGATG ACTACATCTT CTTCAATATC ACAAAGCCTC CACAGGCTGG GGAGATCATG 
AAGAAGCCAG GGCCAGGACT GATAGGCTAT CCTGTCCATG GCTTCCTTCA GAGGGATTTG 
TTTAATGGAA TCATTTATTA TCGTCATTTT GGTGGAGAAA TCTTTGAAGA TTCTTTTCAA 
TTTGTCCTGT GGGACAGCCA TGAACCTCCA AATCTTTCAG TGCCACAGGT GGCAACAATC 
CATATAACTC CAGTGGATGA CCAGCTTCCA AAAGAGGCTC CTGGAGTTTC TCGGCATTTG 
GTTGTCAAGG AAACTGAGGT GGCCTATATA ACTAAGAAAC AGCTACATTT TATAGATTCA 
GAATCATATG ACAGGGAGCT GGTCTACACA ATAACTACTC CTCCATTTTT CTCCTTCAGC 
CACAGACACT TGGATGCTGG GAAATTATTT ATGGTGGACA GCATACCAAA AGTAGTTAAG 
AATCCTACGG CCCTGGAGCT GAGGTCATTC ACTCAGCATG CTGTGAACTA TATGAAAGTG 
GCCTACATGC CCCCCATGCA AGACATTGGT CCCCATTGCA GAGATGTCCA GTTCACATTT 
TCTGTCAGTA ACCAACATGG CGGTACTTTG CATGGGATCT GCTTTAACAT TACAATCCTC 
CCAGTGGACA ATCAAGTTCC TGAGGCGTTC ACCAACCCTC TGAAAGTGAC TGAGGGAGGT 
CAAAGCATCA TCAGCACAGA GCACATTCTA ATTTCTGATG CAGATACCAA GCTGGACAAT 
ATTGACCTCT CCCTGCGGGA ATTGCCTCTG CACGGAAGGG TGGAGCTGAA TGGATTTCCT 
CTAAATTCAG GGGGCACATT TTCTTGGGGC GATCTCCATA CCTTAAAAGT TAGGTATCAA 
CATGATGGAA CTGAAGTTCT TCAGGATGAC CTACTCTTGG AGGTCACCGA TGGCACAAAT 
TCAGCAGAAT TTGTACTACA TGTTGAGGTA TTCCCTGTCA ACGATGAGCC ACCAGTCTTA 
AAGGCTGACC TCATGCCTGT CATGAATTGC TCAGAGGGAG GAGAGGTGGT CATCACCTCT 
GAATACATTT TTGCTACTGA TGTGGACAGC GATAACTTGA AGTTGATGTT TGTGATTGCT 
CGCGAACCTC AGCATGGGGT GGTGAGGAGA GCTGGAGTCA CAGTGGATCA GTTCTCTCAG 
AGAGATGTTA TCTCAGAGGC CGTGACATAC AAACACACAG GTGGCGAGAT TGGCCTGATG 
CCTTGTTTTG ACACCATTAC ATTGGTGGTT TCGGATGGAG AGGCTGGCCC TTTTGTGAAT 
GGCTGTTGCT ACAATGGACC CAATCCATCT GTTCCACTTC ATGCGTCCTT TCCAGTGTAT 
GATCTCAACA TCACGGTATA CCCAGTAGAC AACCAGCCAC CTTCCATTGC AATAGGTCCC 
GTGTTTGTTG TAGATGAGGG CTGCTCAACA GCCCTTACTG TTAACCATTT GTCCGCCACT 
GACCCTGACA CTGCAGCAGA TGACTTGGAA TTTGTTTTGG TTTCTCCTCC TCAGTTTGGC 
TACCTCGAAA ATATACTCCC TTCTGTGGGT TTTGAAAAAA GCAATATTGG CATAAGTATA 
GATTCATTTC AGTGGAAAGA CATGAACGCT TTTCACATTA ACTATGTGCA GTCCAGGCAT 
CTGAGGATAG AACCAACTGC CGACCAGTTC ACGGTGTACG TCACAGATGG GAAGCATCAC 
TCCTTGGAGA TACCATTTTC TATTATAATC AACCCCACAA ATGATGAAGC TCCTGACTTT 
GTAGTGCAGA ATATTACTGT GTGTGAGGGT CAGATGAAAG AGCTGGACTC TTCCATCATC 
AGCGCTGTGG ACCTGGACAT TCCCCAGGAT GCCCTGCTGT TCAGCATCAC TCAAAAGCCA 
CGCCATGGCC TCCTCATCGA TAGGGGGTTT AGCAAAGACT TCTCTGAGAA TAAGCAGCCA 
GCCAACCCTC ACCAGAAACA TGCACCTGTT CACAGCTTTT CCATGGAACT CCTCAAGACT 
GGAATGAGGT TGACGTACAT GCATGATGAC TCAGAGAGCC TTGCTGATGA TTTTACAATC 
CAATTGTCAG ATGGGAAACA TAAGATACTT AAAACCATTT CAGTAGAGGT CATCCCAGTT 
AATGATGAAA AACCAATGCT GAGCAAGAAG GCTGAAATTG CAATGAATAT GGGTGAAACT 
CGTATTATTT CCAGTGCTAT TCTTTCAGCC ATAGATGAAG ACTCACCCAG GGAGAAGATT 
TACTATGTAT TTGAAAGGCT TCCCCAAAAT GGGCAACTTC AGCTTAAGGC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016869819.1
CDS	1065..5078
Translation	MNSLSWGAANAVLLLLLLAWASPTFISINRGVRVMKGHSAFLSGDDLKFAIPKEKDACKVEVVMNEPITQRVGKLTPQVFDCHFLPNEVKYVHNGCPILDEDTVKLRLYRFTERDTFIETFILWVYLLEPDCNIIHMSNNVLEVPEFNGLSQAIDKNLLRFDYDRMASLECTVSLDTARTRLPAHGQMVLGEPRPEEPRGDQPHSFFPESRMMSFNAFLELRAKLKCPGGSCTPGLKKIGSLKVSCEEFLLMGLRYQHLDPPSPNIDYISIQLDLTDTRSKIVYKSESAWLPVYIRAGIPNQIPKAAFMAVFILEVDQFILTSLTTSVLDCEEDETPKPLLVFNITKAPLQGYVTHLLDHTRPISSFTWKDLSDMQIAYQPPNSSHSERRHDEVELEVYDFFFERSAPMTVHISIRTADTNAPRVSWNTGLSLLEGQSRAITWEQFQVVDNDDIGAVRLVTVGGLQHGWLTLRGGKGFLFTVADLQAGVVRYHHDDSDSTKDFVVFRIFDGHHSIRHKFPINVLPKDDSPPFLITNVVIELEEGQTILIQGSMLRASDVDASDDYIFFNITKPPQAGEIMKKPGPGLIGYPVHGFLQRDLFNGIIYYRHFGGEIFEDSFQFVLWDSHEPPNLSVPQVATIHITPVDDQLPKEAPGVSRHLVVKETEVAYITKKQLHFIDSESYDRELVYTITTPPFFSFSHRHLDAGKLFMVDSIPKVVKNPTALELRSFTQHAVNYMKVAYMPPMQDIGPHCRDVQFTFSVSNQHGGTLHGICFNITILPVDNQVPEAFTNPLKVTEGGQSIISTEHILISDADTKLDNIDLSLRELPLHGRVELNGFPLNSGGTFSWGDLHTLKVRYQHDGTEVLQDDLLLEVTDGTNSAEFVLHVEVFPVNDEPPVLKADLMPVMNCSEGGEVVITSEYIFATDVDSDNLKLMFVIAREPQHGVVRRAGVTVDQFSQRDVISEAVTYKHTGGEIGLMPCFDTITLVVSDGEAGPFVNGCCYNGPNPSVPLHASFPVYDLNITVYPVDNQPPSIAIGPVFVVDEGCSTALTVNHLSATDPDTAADDLEFVLVSPPQFGYLENILPSVGFEKSNIGISIDSFQWKDMNAFHINYVQSRHLRIEPTADQFTVYVTDGKHHSLEIPFSIIINPTNDEAPDFVVQNITVCEGQMKELDSSIISAVDLDIPQDALLFSITQKPRHGLLIDRGFSKDFSENKQPANPHQKHAPVHSFSMELLKTGMRLTYMHDDSESLADDFTIQLSDGKHKILKTISVEVIPVNDEKPMLSKKAEIAMNMGETRIISSAILSAIDEDSPREKIYYVFERLPQNGQLQLKA

Target ORF information:

RefSeq Version	XM_017014330.1
Organism	Homo sapiens(human)
Definition	Homo sapiens FRAS1 related extracellular matrix 1 (FREM1), transcript variant X20, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017014330.1

ORF Insert Sequence:

ATGAACTCTC TGAGTTGGGG GGCTGCGAAT GCCGTGCTGC TGCTGCTCCT CCTGGCCTGG 
GCCAGCCCCA CCTTCATCAG CATCAACCGC GGGGTGAGGG TGATGAAGGG CCACTCTGCC 
TTCCTGTCAG GAGATGACCT GAAGTTTGCC ATCCCTAAAG AGAAAGATGC CTGCAAAGTG 
GAAGTTGTGA TGAATGAGCC AATAACCCAG AGGGTTGGGA AACTCACTCC ACAGGTCTTT 
GACTGCCATT TCCTTCCCAA CGAAGTCAAG TATGTTCACA ATGGTTGTCC AATTCTTGAT 
GAAGACACAG TGAAGCTCAG ACTTTACAGA TTTACTGAAA GAGATACCTT CATAGAAACT 
TTTATCCTGT GGGTCTATCT CCTGGAACCA GACTGTAACA TCATCCATAT GAGTAACAAT 
GTGCTGGAGG TGCCTGAATT CAATGGCTTG TCCCAAGCGA TTGATAAAAA TCTGCTCAGA 
TTCGATTATG ATAGGATGGC TAGCCTGGAA TGTACCGTCA GCCTGGACAC TGCGAGAACT 
CGGCTGCCAG CCCATGGCCA GATGGTTCTC GGGGAGCCTC GACCAGAAGA ACCTCGTGGA 
GATCAGCCAC ACAGTTTTTT CCCAGAGTCC CGTATGATGT CTTTTAATGC CTTTCTGGAA 
CTGAGAGCAA AACTGAAGTG TCCAGGTGGG AGCTGTACCC CAGGATTAAA GAAAATAGGA 
AGCCTCAAAG TGAGCTGTGA GGAGTTCCTG CTGATGGGCC TTCGTTATCA GCATCTGGAT 
CCCCCTTCAC CCAACATTGA TTATATCTCC ATCCAACTGG ACCTCACAGA TACCAGGAGC 
AAAATTGTGT ACAAGTCAGA GAGTGCGTGG CTGCCTGTCT ATATCAGAGC TGGAATTCCG 
AATCAGATTC CAAAGGCTGC ATTCATGGCC GTGTTTATTC TGGAAGTGGA TCAGTTCATC 
CTGACCTCCT TGACTACATC AGTTCTGGAC TGTGAAGAAG ATGAGACCCC TAAACCCTTG 
CTGGTGTTCA ACATTACTAA AGCCCCGCTC CAGGGCTATG TGACTCACCT GTTGGATCAC 
ACCAGACCAA TCTCCTCATT CACCTGGAAA GATCTCAGTG ACATGCAGAT CGCCTATCAG 
CCACCAAACA GCAGCCATTC TGAGAGGAGA CATGATGAGG TAGAATTGGA GGTATACGAC 
TTCTTCTTTG AAAGGAGTGC ACCTATGACA GTCCACATCT CCATCAGAAC AGCAGATACA 
AATGCCCCCC GTGTATCCTG GAATACAGGT CTGAGTCTCC TTGAGGGGCA GTCTCGAGCC 
ATCACTTGGG AACAGTTTCA GGTTGTCGAC AATGACGACA TTGGTGCTGT CCGGCTAGTC 
ACCGTTGGTG GCCTGCAGCA TGGATGGCTG ACTTTAAGAG GGGGGAAAGG GTTTCTCTTC 
ACCGTGGCTG ACCTCCAGGC TGGAGTTGTT CGCTATCATC ATGATGACAG CGACTCCACC 
AAAGACTTCG TGGTCTTCCG GATATTTGAT GGCCATCACA GCATCCGTCA CAAATTCCCC 
ATCAACGTCT TGCCCAAAGA TGATAGTCCC CCGTTCCTCA TAACCAATGT TGTGATTGAA 
CTGGAGGAGG GGCAGACCAT CCTGATCCAG GGATCCATGC TGCGAGCTTC AGATGTGGAC 
GCCAGTGATG ACTACATCTT CTTCAATATC ACAAAGCCTC CACAGGCTGG GGAGATCATG 
AAGAAGCCAG GGCCAGGACT GATAGGCTAT CCTGTCCATG GCTTCCTTCA GAGGGATTTG 
TTTAATGGAA TCATTTATTA TCGTCATTTT GGTGGAGAAA TCTTTGAAGA TTCTTTTCAA 
TTTGTCCTGT GGGACAGCCA TGAACCTCCA AATCTTTCAG TGCCACAGGT GGCAACAATC 
CATATAACTC CAGTGGATGA CCAGCTTCCA AAAGAGGCTC CTGGAGTTTC TCGGCATTTG 
GTTGTCAAGG AAACTGAGGT GGCCTATATA ACTAAGAAAC AGCTACATTT TATAGATTCA 
GAATCATATG ACAGGGAGCT GGTCTACACA ATAACTACTC CTCCATTTTT CTCCTTCAGC 
CACAGACACT TGGATGCTGG GAAATTATTT ATGGTGGACA GCATACCAAA AGTAGTTAAG 
AATCCTACGG CCCTGGAGCT GAGGTCATTC ACTCAGCATG CTGTGAACTA TATGAAAGTG 
GCCTACATGC CCCCCATGCA AGACATTGGT CCCCATTGCA GAGATGTCCA GTTCACATTT 
TCTGTCAGTA ACCAACATGG CGGTACTTTG CATGGGATCT GCTTTAACAT TACAATCCTC 
CCAGTGGACA ATCAAGTTCC TGAGGCGTTC ACCAACCCTC TGAAAGTGAC TGAGGGAGGT 
CAAAGCATCA TCAGCACAGA GCACATTCTA ATTTCTGATG CAGATACCAA GCTGGACAAT 
ATTGACCTCT CCCTGCGGGA ATTGCCTCTG CACGGAAGGG TGGAGCTGAA TGGATTTCCT 
CTAAATTCAG GGGGCACATT TTCTTGGGGC GATCTCCATA CCTTAAAAGT TAGGTATCAA 
CATGATGGAA CTGAAGTTCT TCAGGATGAC CTACTCTTGG AGGTCACCGA TGGCACAAAT 
TCAGCAGAAT TTGTACTACA TGTTGAGGTA TTCCCTGTCA ACGATGAGCC ACCAGTCTTA 
AAGGCTGACC TCATGCCTGT CATGAATTGC TCAGAGGGAG GAGAGGTGGT CATCACCTCT 
GAATACATTT TTGCTACTGA TGTGGACAGC GATAACTTGA AGTTGATGTT TGTGATTGCT 
CGCGAACCTC AGCATGGGGT GGTGAGGAGA GCTGGAGTCA CAGTGGATCA GTTCTCTCAG 
AGAGATGTTA TCTCAGAGGC CGTGACATAC AAACACACAG GTGGCGAGAT TGGCCTGATG 
CCTTGTTTTG ACACCATTAC ATTGGTGGTT TCGGATGGAG AGGCTGGCCC TTTTGTGAAT 
GGCTGTTGCT ACAATGGACC CAATCCATCT GTTCCACTTC ATGCGTCCTT TCCAGTGTAT 
GATCTCAACA TCACGGTATA CCCAGTAGAC AACCAGCCAC CTTCCATTGC AATAGGTCCC 
GTGTTTGTTG TAGATGAGGG CTGCTCAACA GCCCTTACTG TTAACCATTT GTCCGCCACT 
GACCCTGACA CTGCAGCAGA TGACTTGGAA TTTGTTTTGG TTTCTCCTCC TCAGTTTGGC 
TACCTCGAAA ATATACTCCC TTCTGTGGGT TTTGAAAAAA GCAATATTGG CATAAGTATA 
GATTCATTTC AGTGGAAAGA CATGAACGCT TTTCACATTA ACTATGTGCA GTCCAGGCAT 
CTGAGGATAG AACCAACTGC CGACCAGTTC ACGGTGTACG TCACAGATGG GAAGCATCAC 
TCCTTGGAGA TACCATTTTC TATTATAATC AACCCCACAA ATGATGAAGC TCCTGACTTT 
GTAGTGCAGA ATATTACTGT GTGTGAGGGT CAGATGAAAG AGCTGGACTC TTCCATCATC 
AGCGCTGTGG ACCTGGACAT TCCCCAGGAT GCCCTGCTGT TCAGCATCAC TCAAAAGCCA 
CGCCATGGCC TCCTCATCGA TAGGGGGTTT AGCAAAGACT TCTCTGAGAA TAAGCAGCCA 
GCCAACCCTC ACCAGAAACA TGCACCTGTT CACAGCTTTT CCATGGAACT CCTCAAGACT 
GGAATGAGGT TGACGTACAT GCATGATGAC TCAGAGAGCC TTGCTGATGA TTTTACAATC 
CAATTGTCAG ATGGGAAACA TAAGATACTT AAAACCATTT CAGTAGAGGT CATCCCAGTT 
AATGATGAAA AACCAATGCT GAGCAAGAAG GCTGAAATTG CAATGAATAT GGGTGAAACT 
CGTATTATTT CCAGTGCTAT TCTTTCAGCC ATAGATGAAG ACTCACCCAG GGAGAAGATT 
TACTATGTAT TTGAAAGGCT TCCCCAAAAT GGGCAACTTC AGCTTAAGGC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FREM1 ( XM_017014327.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016869816.1 Homo sapiens FRAS1 related extracellular matrix 1 (FREM1), transcript variant X13, mRNA.

Price & Availability↑

CloneID	OHu98463
Clone ID Related Accession (Same CDS sequence)	XM_017014327.1 , XM_017014327.2
Accession Version	XM_017014327.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5643bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	FRAS1-related extracellular matrix protein 1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGTGT TTATTCTGGA AGTGGATCAG TTCATCCTGA CCTCCTTGAC TACATCAGTT 
CTGGACTGTG AAGAAGATGA GACCCCTAAA CCCTTGCTGG TGTTCAACAT TACTAAAGCC 
CCGCTCCAGG GCTATGTGAC TCACCTGTTG GATCACACCA GACCAATCTC CTCATTCACC 
TGGAAAGATC TCAGTGACAT GCAGATCGCC TATCAGCCAC CAAACAGCAG CCATTCTGAG 
AGGAGACATG ATGAGGTAGA ATTGGAGGTA TACGACTTCT TCTTTGAAAG GAGTGCACCT 
ATGACAGTCC ACATCTCCAT CAGAACAGCA GATACAAATG CCCCCCGTGT ATCCTGGAAT 
ACAGGTCTGA GTCTCCTTGA GGGGCAGTCT CGAGCCATCA CTTGGGAACA GTTTCAGGTT 
GTCGACAATG ACGACATTGG TGCTGTCCGG CTAGTCACCG TTGGTGGCCT GCAGCATGGA 
TGGCTGACTT TAAGAGGGGG GAAAGGGTTT CTCTTCACCG TGGCTGACCT CCAGGCTGGA 
GTTGTTCGCT ATCATCATGA TGACAGCGAC TCCACCAAAG ACTTCGTGGT CTTCCGGATA 
TTTGATGGCC ATCACAGCAT CCGTCACAAA TTCCCCATCA ACGTCTTGCC CAAAGATGAT 
AGTCCCCCGT TCCTCATAAC CAATGTTGTG ATTGAACTGG AGGAGGGGCA GACCATCCTG 
ATCCAGGGAT CCATGCTGCG AGCTTCAGAT GTGGACGCCA GTGATGACTA CATCTTCTTC 
AATATCACAA AGCCTCCACA GGCTGGGGAG ATCATGAAGA AGCCAGGGCC AGGACTGATA 
GGCTATCCTG TCCATGGCTT CCTTCAGAGG GATTTGTTTA ATGGAATCAT TTATTATCGT 
CATTTTGGTG GAGAAATCTT TGAAGATTCT TTTCAATTTG TCCTGTGGGA CAGCCATGAA 
CCTCCAAATC TTTCAGTGCC ACAGGTGGCA ACAATCCATA TAACTCCAGT GGATGACCAG 
CTTCCAAAAG AGGCTCCTGG AGTTTCTCGG CATTTGGTTG TCAAGGAAAC TGAGGTGGCC 
TATATAACTA AGAAACAGCT ACATTTTATA GATTCAGAAT CATATGACAG GGAGCTGGTC 
TACACAATAA CTACTCCTCC ATTTTTCTCC TTCAGCCACA GACACTTGGA TGCTGGGAAA 
TTATTTATGG TGGACAGCAT ACCAAAAGTA GTTAAGAATC CTACGGCCCT GGAGCTGAGG 
TCATTCACTC AGCATGCTGT GAACTATATG AAAGTGGCCT ACATGCCCCC CATGCAAGAC 
ATTGGTCCCC ATTGCAGAGA TGTCCAGTTC ACATTTTCTG TCAGTAACCA ACATGGCGGT 
ACTTTGCATG GGATCTGCTT TAACATTACA ATCCTCCCAG TGGACAATCA AGTTCCTGAG 
GCGTTCACCA ACCCTCTGAA AGTGACTGAG GGAGGTCAAA GCATCATCAG CACAGAGCAC 
ATTCTAATTT CTGATGCAGA TACCAAGCTG GACAATATTG ACCTCTCCCT GCGGGAATTG 
CCTCTGCACG GAAGGGTGGA GCTGAATGGA TTTCCTCTAA ATTCAGGGGG CACATTTTCT 
TGGGGCGATC TCCATACCTT AAAAGTTAGG TATCAACATG ATGGAACTGA AGTTCTTCAG 
GATGACCTAC TCTTGGAGGT CACCGATGGC ACAAATTCAG CAGAATTTGT ACTACATGTT 
GAGGTATTCC CTGTCAACGA TGAGCCACCA GTCTTAAAGG CTGACCTCAT GCCTGTCATG 
AATTGCTCAG AGGGAGGAGA GGTGGTCATC ACCTCTGAAT ACATTTTTGC TACTGATGTG 
GACAGCGATA ACTTGAAGTT GATGTTTGTG ATTGCTCGCG AACCTCAGCA TGGGGTGGTG 
AGGAGAGCTG GAGTCACAGT GGATCAGTTC TCTCAGAGAG ATGTTATCTC AGAGGCCGTG 
ACATACAAAC ACACAGGTGG CGAGATTGGC CTGATGCCTT GTTTTGACAC CATTACATTG 
GTGGTTTCGG ATGGAGAGGC TGGCCCTTTT GTGAATGGCT GTTGCTACAA TGGACCCAAT 
CCATCTGTTC CACTTCATGC GTCCTTTCCA GTGTATGATC TCAACATCAC GGTATACCCA 
GTAGACAACC AGCCACCTTC CATTGCAATA GGTCCCGTGT TTGTTGTAGA TGAGGGCTGC 
TCAACAGCCC TTACTGTTAA CCATTTGTCC GCCACTGACC CTGACACTGC AGCAGATGAC 
TTGGAATTTG TTTTGGTTTC TCCTCCTCAG TTTGGCTACC TCGAAAATAT ACTCCCTTCT 
GTGGGTTTTG AAAAAAGCAA TATTGGCATA AGTATAGATT CATTTCAGTG GAAAGACATG 
AACGCTTTTC ACATTAACTA TGTGCAGTCC AGGCATCTGA GGATAGAACC AACTGCCGAC 
CAGTTCACGG TGTACGTCAC AGATGGGAAG CATCACTCCT TGGAGATACC ATTTTCTATT 
ATAATCAACC CCACAAATGA TGAAGCTCCT GACTTTGTAG TGCAGAATAT TACTGTGTGT 
GAGGGTCAGA TGAAAGAGCT GGACTCTTCC ATCATCAGCG CTGTGGACCT GGACATTCCC 
CAGGATGCCC TGCTGTTCAG CATCACTCAA AAGCCACGCC ATGGCCTCCT CATCGATAGG 
GGGTTTAGCA AAGACTTCTC TGAGAATAAG CAGCCAGCCA ACCCTCACCA GAAACATGCA 
CCTGTTCACA GCTTTTCCAT GGAACTCCTC AAGACTGGAA TGAGGTTGAC GTACATGCAT 
GATGACTCAG AGAGCCTTGC TGATGATTTT ACAATCCAAT TGTCAGATGG GAAACATAAG 
ATACTTAAAA CCATTTCAGT AGAGGTCATC CCAGTTAATG ATGAAAAACC AATGCTGAGC 
AAGAAGGCTG AAATTGCAAT GAATATGGGT GAAACTCGTA TTATTTCCAG TGCTATTCTT 
TCAGCCATAG ATGAAGACTC ACCCAGGGAG AAGATTTACT ATGTATTTGA AAGGCTTCCC 
CAAAATGGGC AACTTCAGCT TAAGATAGGG AGGGACTGGG TTCCTCTCTC CCCTGGCATG 
AAATGCACTC AGGAGGAAGT GGATCTGAAC TTGCTGAGAT ACACACACAC CGGGGCAATG 
GATTCCCAGA ATCAAGATAG CTTCACCTTC TACCTTTGGG ATGGCAACAA CAGGTCCCCT 
GCTCTTGACT GTCAAATCAC CATCAAGGAC ATGGAAAAAG GTGATATTGT CATCCTTACA 
AAACCACTCG TGGTGTCTAA AGGTGATAGA GGTTTCTTAA CAACCACCAC GCTCCTGGCT 
GTGGACGGAA CAGACAAGCC TGAGGAACTG CTCTATGTCA TCACCTCCCC TCCGCGATAT 
GGCCAGATCG AATATGTTCA CTATCCTGGA GTTCCCATTA CAAACTTCAG CCAAATGGAT 
GTAGTGGGGC AGACAGTTTG CTATGTACAC AAGAGCAAGG TGACTGTCTC CAGTGACAGA 
TTCAGATTCA TCATCAGCAA TGGACTGCGG ACCGAGCACG GGGTGTTTGA GATCACACTG 
GAGACTGTGG ACAGAGCCCT GCCTGTGGTA ACCAGGAACA AGGGGTTGAG ACTGGCCCAA 
GGGGCCGTGG GCCTGCTTTC CCCTGACCTC CTTCAGCTGA CCGACCCTGA TACACCTGCG 
GAGAACCTCA CCTTCCTCTT GGTTCAGCTC CCCCAGCATG GCCAGCTCTA CCTGTGGGGG 
ACAGGGCTAC TTCAACACAA TTTCACCCAG CAGGATGTGG ACAGCAAGAA TGTGGCCTAT 
CGGCACTCAG GAGGGGACTC CCAGACTGAC TGCTTTACTT TCATGGCCAC AGATGGGACA 
AACCAAGGCT TTATTGTGAA TGGGAGAGTG TGGGAAGAAC CTGTTTTATT CACCATTCAG 
GTAGACCAAT TGGACAAAAC AGCTCCTCGT ATCACACTCT TGCATTCCCC TTCTCAAGTG 
GGGCTCCTGA AAAATGGCTG CTACGGGATT TACATCACTT CCCGCGTGTT GAAGGCATCA 
GACCCTGACA CTGAGGACGA TCAGATCATC TTTAAAATTC TACAAGGCCC AAAACATGGA 
CATCTGGAGA ACACAACAAC AGGTGAATTT ATCCATGAGA AATTTAGCCA AAAGGACTTA 
AACAGTAAGA CTATTCTTTA CATCATAAAC CCATCTTTGG AAGTAAATTC AGATACCGTG 
GAATTTCAAA TCATGGACCC CACAGGGAAC TCGGCCACTC CTCAAATTTT GGAACTGAAG 
TGGTCTCATA TTGAATGGTC ACAGACCGAA TATGAAGTCT GTGAGAATGT GGGTTTGTTG 
CCCTTGGAAA TTATCAGAAG GGGATATTCC ATGGACTCGG CCTTTGTGGG TATAAAGGTC 
AACCAAGTGT CAGCTGCAGT TGGAAAAGAT TTCACCGTGA TTCCATCTAA ACTGATTCAG 
TTTGACCCAG GAATGTCAAC TAAGATGTGG AATATAGCAA TTACCTATGA CGGATTAGAG 
GAAGATGATG AGGTCTTTGA AGTAATTCTG AACTCCCCTG TGAATGCAGT TCTTGGCACA 
AAGACAAAAG CTGCAGTGAA AATTTTGGAC TCAAAAGGAG GACAATGCCA TCCTTCATAT 
TCCTCCAACC AAAGCAAGCA CAGCACATGG GAGAAGGGCA TTTGGCATCT GCTGCCCCCA 
GGGTCTTCCT CATCCACCAC TTCTGGTTCC TTTCATCTGG AAAGAAGACC TCTTCCATCT 
TCCATGCAGC TAGCAGTCAT CAGGGGAGAC ACCCTGCGGG GCTTTGATTC TACAGATCTT 
TCTCAAAGGA AGCTTAGGAC CCGTGGGAAT GGCAAAACAG TTCGTCCATC CTCTGTTTAT 
AGAAATGGAA CAGACATCAT CTATAATTAT CATGGGATAG TTTCCTTGAA ACTGGAGGAT 
GACAGTTTCC CAACTCACAA AAGGAAGGCC AAAGTATCCA TCATTAGTCA GCCACAAAAG 
ACAATCAAAG TGGCAGAACT GCCTCAAGCA GATAAGGTGG AATCCACAAC TGACTCACAC 
TTCCCCAGAC AGGACCAGTT GCCCTCATTT CCAAAGAACT GCACTCTGGA ATTAAAGGGA 
CTCTTCCATT TTGAAGAAGG CATCCAGAAG CTGTATCAGT GCAATGGGAT CGCCTGGAAA 
GCCTGGAGTC CCCAAACCAA GGATGTGGAA GACAAATCCT GTCCAGCCGG GTGGCACCAG 
CACTCAGGCT ACTGTCACAT CTTGATCACA GAGCAGAAAG GCACCTGGAA TGCGGCTGCC 
CAAGCTTGCA GGGAACAATA CCTGGGCAAC CTTGTAACTG TATTCTCCAG GCAGCACATG 
CGGTGGCTCT GGGACATTGG TGGGAGAAAG TCCTTTTGGA TAGGTTTGAA CGACCAAGTG 
CATGCTGGCC ACTGGGAGTG GATCGGTGGT GAACCTGTTG CCTTCACCAA TGGGAGAAGA 
GGGCCCTCTC AACGCTCCAA GCTTGGAAAG AGCTGTGTTT TGGTTCAAAG ACAAGGGAAA 
TGGCAAACAA AAGACTGTAG GAGAGCCAAA CCTCATAATT ATGTGTGTTC CAGAAAACTC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016869816.1
CDS	174..5816
Translation	MAVFILEVDQFILTSLTTSVLDCEEDETPKPLLVFNITKAPLQGYVTHLLDHTRPISSFTWKDLSDMQIAYQPPNSSHSERRHDEVELEVYDFFFERSAPMTVHISIRTADTNAPRVSWNTGLSLLEGQSRAITWEQFQVVDNDDIGAVRLVTVGGLQHGWLTLRGGKGFLFTVADLQAGVVRYHHDDSDSTKDFVVFRIFDGHHSIRHKFPINVLPKDDSPPFLITNVVIELEEGQTILIQGSMLRASDVDASDDYIFFNITKPPQAGEIMKKPGPGLIGYPVHGFLQRDLFNGIIYYRHFGGEIFEDSFQFVLWDSHEPPNLSVPQVATIHITPVDDQLPKEAPGVSRHLVVKETEVAYITKKQLHFIDSESYDRELVYTITTPPFFSFSHRHLDAGKLFMVDSIPKVVKNPTALELRSFTQHAVNYMKVAYMPPMQDIGPHCRDVQFTFSVSNQHGGTLHGICFNITILPVDNQVPEAFTNPLKVTEGGQSIISTEHILISDADTKLDNIDLSLRELPLHGRVELNGFPLNSGGTFSWGDLHTLKVRYQHDGTEVLQDDLLLEVTDGTNSAEFVLHVEVFPVNDEPPVLKADLMPVMNCSEGGEVVITSEYIFATDVDSDNLKLMFVIAREPQHGVVRRAGVTVDQFSQRDVISEAVTYKHTGGEIGLMPCFDTITLVVSDGEAGPFVNGCCYNGPNPSVPLHASFPVYDLNITVYPVDNQPPSIAIGPVFVVDEGCSTALTVNHLSATDPDTAADDLEFVLVSPPQFGYLENILPSVGFEKSNIGISIDSFQWKDMNAFHINYVQSRHLRIEPTADQFTVYVTDGKHHSLEIPFSIIINPTNDEAPDFVVQNITVCEGQMKELDSSIISAVDLDIPQDALLFSITQKPRHGLLIDRGFSKDFSENKQPANPHQKHAPVHSFSMELLKTGMRLTYMHDDSESLADDFTIQLSDGKHKILKTISVEVIPVNDEKPMLSKKAEIAMNMGETRIISSAILSAIDEDSPREKIYYVFERLPQNGQLQLKIGRDWVPLSPGMKCTQEEVDLNLLRYTHTGAMDSQNQDSFTFYLWDGNNRSPALDCQITIKDMEKGDIVILTKPLVVSKGDRGFLTTTTLLAVDGTDKPEELLYVITSPPRYGQIEYVHYPGVPITNFSQMDVVGQTVCYVHKSKVTVSSDRFRFIISNGLRTEHGVFEITLETVDRALPVVTRNKGLRLAQGAVGLLSPDLLQLTDPDTPAENLTFLLVQLPQHGQLYLWGTGLLQHNFTQQDVDSKNVAYRHSGGDSQTDCFTFMATDGTNQGFIVNGRVWEEPVLFTIQVDQLDKTAPRITLLHSPSQVGLLKNGCYGIYITSRVLKASDPDTEDDQIIFKILQGPKHGHLENTTTGEFIHEKFSQKDLNSKTILYIINPSLEVNSDTVEFQIMDPTGNSATPQILELKWSHIEWSQTEYEVCENVGLLPLEIIRRGYSMDSAFVGIKVNQVSAAVGKDFTVIPSKLIQFDPGMSTKMWNIAITYDGLEEDDEVFEVILNSPVNAVLGTKTKAAVKILDSKGGQCHPSYSSNQSKHSTWEKGIWHLLPPGSSSSTTSGSFHLERRPLPSSMQLAVIRGDTLRGFDSTDLSQRKLRTRGNGKTVRPSSVYRNGTDIIYNYHGIVSLKLEDDSFPTHKRKAKVSIISQPQKTIKVAELPQADKVESTTDSHFPRQDQLPSFPKNCTLELKGLFHFEEGIQKLYQCNGIAWKAWSPQTKDVEDKSCPAGWHQHSGYCHILITEQKGTWNAAAQACREQYLGNLVTVFSRQHMRWLWDIGGRKSFWIGLNDQVHAGHWEWIGGEPVAFTNGRRGPSQRSKLGKSCVLVQRQGKWQTKDCRRAKPHNYVCSRKL

Target ORF information:

RefSeq Version	XM_017014327.1
Organism	Homo sapiens(human)
Definition	Homo sapiens FRAS1 related extracellular matrix 1 (FREM1), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017014327.1

ORF Insert Sequence:

ATGGCCGTGT TTATTCTGGA AGTGGATCAG TTCATCCTGA CCTCCTTGAC TACATCAGTT 
CTGGACTGTG AAGAAGATGA GACCCCTAAA CCCTTGCTGG TGTTCAACAT TACTAAAGCC 
CCGCTCCAGG GCTATGTGAC TCACCTGTTG GATCACACCA GACCAATCTC CTCATTCACC 
TGGAAAGATC TCAGTGACAT GCAGATCGCC TATCAGCCAC CAAACAGCAG CCATTCTGAG 
AGGAGACATG ATGAGGTAGA ATTGGAGGTA TACGACTTCT TCTTTGAAAG GAGTGCACCT 
ATGACAGTCC ACATCTCCAT CAGAACAGCA GATACAAATG CCCCCCGTGT ATCCTGGAAT 
ACAGGTCTGA GTCTCCTTGA GGGGCAGTCT CGAGCCATCA CTTGGGAACA GTTTCAGGTT 
GTCGACAATG ACGACATTGG TGCTGTCCGG CTAGTCACCG TTGGTGGCCT GCAGCATGGA 
TGGCTGACTT TAAGAGGGGG GAAAGGGTTT CTCTTCACCG TGGCTGACCT CCAGGCTGGA 
GTTGTTCGCT ATCATCATGA TGACAGCGAC TCCACCAAAG ACTTCGTGGT CTTCCGGATA 
TTTGATGGCC ATCACAGCAT CCGTCACAAA TTCCCCATCA ACGTCTTGCC CAAAGATGAT 
AGTCCCCCGT TCCTCATAAC CAATGTTGTG ATTGAACTGG AGGAGGGGCA GACCATCCTG 
ATCCAGGGAT CCATGCTGCG AGCTTCAGAT GTGGACGCCA GTGATGACTA CATCTTCTTC 
AATATCACAA AGCCTCCACA GGCTGGGGAG ATCATGAAGA AGCCAGGGCC AGGACTGATA 
GGCTATCCTG TCCATGGCTT CCTTCAGAGG GATTTGTTTA ATGGAATCAT TTATTATCGT 
CATTTTGGTG GAGAAATCTT TGAAGATTCT TTTCAATTTG TCCTGTGGGA CAGCCATGAA 
CCTCCAAATC TTTCAGTGCC ACAGGTGGCA ACAATCCATA TAACTCCAGT GGATGACCAG 
CTTCCAAAAG AGGCTCCTGG AGTTTCTCGG CATTTGGTTG TCAAGGAAAC TGAGGTGGCC 
TATATAACTA AGAAACAGCT ACATTTTATA GATTCAGAAT CATATGACAG GGAGCTGGTC 
TACACAATAA CTACTCCTCC ATTTTTCTCC TTCAGCCACA GACACTTGGA TGCTGGGAAA 
TTATTTATGG TGGACAGCAT ACCAAAAGTA GTTAAGAATC CTACGGCCCT GGAGCTGAGG 
TCATTCACTC AGCATGCTGT GAACTATATG AAAGTGGCCT ACATGCCCCC CATGCAAGAC 
ATTGGTCCCC ATTGCAGAGA TGTCCAGTTC ACATTTTCTG TCAGTAACCA ACATGGCGGT 
ACTTTGCATG GGATCTGCTT TAACATTACA ATCCTCCCAG TGGACAATCA AGTTCCTGAG 
GCGTTCACCA ACCCTCTGAA AGTGACTGAG GGAGGTCAAA GCATCATCAG CACAGAGCAC 
ATTCTAATTT CTGATGCAGA TACCAAGCTG GACAATATTG ACCTCTCCCT GCGGGAATTG 
CCTCTGCACG GAAGGGTGGA GCTGAATGGA TTTCCTCTAA ATTCAGGGGG CACATTTTCT 
TGGGGCGATC TCCATACCTT AAAAGTTAGG TATCAACATG ATGGAACTGA AGTTCTTCAG 
GATGACCTAC TCTTGGAGGT CACCGATGGC ACAAATTCAG CAGAATTTGT ACTACATGTT 
GAGGTATTCC CTGTCAACGA TGAGCCACCA GTCTTAAAGG CTGACCTCAT GCCTGTCATG 
AATTGCTCAG AGGGAGGAGA GGTGGTCATC ACCTCTGAAT ACATTTTTGC TACTGATGTG 
GACAGCGATA ACTTGAAGTT GATGTTTGTG ATTGCTCGCG AACCTCAGCA TGGGGTGGTG 
AGGAGAGCTG GAGTCACAGT GGATCAGTTC TCTCAGAGAG ATGTTATCTC AGAGGCCGTG 
ACATACAAAC ACACAGGTGG CGAGATTGGC CTGATGCCTT GTTTTGACAC CATTACATTG 
GTGGTTTCGG ATGGAGAGGC TGGCCCTTTT GTGAATGGCT GTTGCTACAA TGGACCCAAT 
CCATCTGTTC CACTTCATGC GTCCTTTCCA GTGTATGATC TCAACATCAC GGTATACCCA 
GTAGACAACC AGCCACCTTC CATTGCAATA GGTCCCGTGT TTGTTGTAGA TGAGGGCTGC 
TCAACAGCCC TTACTGTTAA CCATTTGTCC GCCACTGACC CTGACACTGC AGCAGATGAC 
TTGGAATTTG TTTTGGTTTC TCCTCCTCAG TTTGGCTACC TCGAAAATAT ACTCCCTTCT 
GTGGGTTTTG AAAAAAGCAA TATTGGCATA AGTATAGATT CATTTCAGTG GAAAGACATG 
AACGCTTTTC ACATTAACTA TGTGCAGTCC AGGCATCTGA GGATAGAACC AACTGCCGAC 
CAGTTCACGG TGTACGTCAC AGATGGGAAG CATCACTCCT TGGAGATACC ATTTTCTATT 
ATAATCAACC CCACAAATGA TGAAGCTCCT GACTTTGTAG TGCAGAATAT TACTGTGTGT 
GAGGGTCAGA TGAAAGAGCT GGACTCTTCC ATCATCAGCG CTGTGGACCT GGACATTCCC 
CAGGATGCCC TGCTGTTCAG CATCACTCAA AAGCCACGCC ATGGCCTCCT CATCGATAGG 
GGGTTTAGCA AAGACTTCTC TGAGAATAAG CAGCCAGCCA ACCCTCACCA GAAACATGCA 
CCTGTTCACA GCTTTTCCAT GGAACTCCTC AAGACTGGAA TGAGGTTGAC GTACATGCAT 
GATGACTCAG AGAGCCTTGC TGATGATTTT ACAATCCAAT TGTCAGATGG GAAACATAAG 
ATACTTAAAA CCATTTCAGT AGAGGTCATC CCAGTTAATG ATGAAAAACC AATGCTGAGC 
AAGAAGGCTG AAATTGCAAT GAATATGGGT GAAACTCGTA TTATTTCCAG TGCTATTCTT 
TCAGCCATAG ATGAAGACTC ACCCAGGGAG AAGATTTACT ATGTATTTGA AAGGCTTCCC 
CAAAATGGGC AACTTCAGCT TAAGATAGGG AGGGACTGGG TTCCTCTCTC CCCTGGCATG 
AAATGCACTC AGGAGGAAGT GGATCTGAAC TTGCTGAGAT ACACACACAC CGGGGCAATG 
GATTCCCAGA ATCAAGATAG CTTCACCTTC TACCTTTGGG ATGGCAACAA CAGGTCCCCT 
GCTCTTGACT GTCAAATCAC CATCAAGGAC ATGGAAAAAG GTGATATTGT CATCCTTACA 
AAACCACTCG TGGTGTCTAA AGGTGATAGA GGTTTCTTAA CAACCACCAC GCTCCTGGCT 
GTGGACGGAA CAGACAAGCC TGAGGAACTG CTCTATGTCA TCACCTCCCC TCCGCGATAT 
GGCCAGATCG AATATGTTCA CTATCCTGGA GTTCCCATTA CAAACTTCAG CCAAATGGAT 
GTAGTGGGGC AGACAGTTTG CTATGTACAC AAGAGCAAGG TGACTGTCTC CAGTGACAGA 
TTCAGATTCA TCATCAGCAA TGGACTGCGG ACCGAGCACG GGGTGTTTGA GATCACACTG 
GAGACTGTGG ACAGAGCCCT GCCTGTGGTA ACCAGGAACA AGGGGTTGAG ACTGGCCCAA 
GGGGCCGTGG GCCTGCTTTC CCCTGACCTC CTTCAGCTGA CCGACCCTGA TACACCTGCG 
GAGAACCTCA CCTTCCTCTT GGTTCAGCTC CCCCAGCATG GCCAGCTCTA CCTGTGGGGG 
ACAGGGCTAC TTCAACACAA TTTCACCCAG CAGGATGTGG ACAGCAAGAA TGTGGCCTAT 
CGGCACTCAG GAGGGGACTC CCAGACTGAC TGCTTTACTT TCATGGCCAC AGATGGGACA 
AACCAAGGCT TTATTGTGAA TGGGAGAGTG TGGGAAGAAC CTGTTTTATT CACCATTCAG 
GTAGACCAAT TGGACAAAAC AGCTCCTCGT ATCACACTCT TGCATTCCCC TTCTCAAGTG 
GGGCTCCTGA AAAATGGCTG CTACGGGATT TACATCACTT CCCGCGTGTT GAAGGCATCA 
GACCCTGACA CTGAGGACGA TCAGATCATC TTTAAAATTC TACAAGGCCC AAAACATGGA 
CATCTGGAGA ACACAACAAC AGGTGAATTT ATCCATGAGA AATTTAGCCA AAAGGACTTA 
AACAGTAAGA CTATTCTTTA CATCATAAAC CCATCTTTGG AAGTAAATTC AGATACCGTG 
GAATTTCAAA TCATGGACCC CACAGGGAAC TCGGCCACTC CTCAAATTTT GGAACTGAAG 
TGGTCTCATA TTGAATGGTC ACAGACCGAA TATGAAGTCT GTGAGAATGT GGGTTTGTTG 
CCCTTGGAAA TTATCAGAAG GGGATATTCC ATGGACTCGG CCTTTGTGGG TATAAAGGTC 
AACCAAGTGT CAGCTGCAGT TGGAAAAGAT TTCACCGTGA TTCCATCTAA ACTGATTCAG 
TTTGACCCAG GAATGTCAAC TAAGATGTGG AATATAGCAA TTACCTATGA CGGATTAGAG 
GAAGATGATG AGGTCTTTGA AGTAATTCTG AACTCCCCTG TGAATGCAGT TCTTGGCACA 
AAGACAAAAG CTGCAGTGAA AATTTTGGAC TCAAAAGGAG GACAATGCCA TCCTTCATAT 
TCCTCCAACC AAAGCAAGCA CAGCACATGG GAGAAGGGCA TTTGGCATCT GCTGCCCCCA 
GGGTCTTCCT CATCCACCAC TTCTGGTTCC TTTCATCTGG AAAGAAGACC TCTTCCATCT 
TCCATGCAGC TAGCAGTCAT CAGGGGAGAC ACCCTGCGGG GCTTTGATTC TACAGATCTT 
TCTCAAAGGA AGCTTAGGAC CCGTGGGAAT GGCAAAACAG TTCGTCCATC CTCTGTTTAT 
AGAAATGGAA CAGACATCAT CTATAATTAT CATGGGATAG TTTCCTTGAA ACTGGAGGAT 
GACAGTTTCC CAACTCACAA AAGGAAGGCC AAAGTATCCA TCATTAGTCA GCCACAAAAG 
ACAATCAAAG TGGCAGAACT GCCTCAAGCA GATAAGGTGG AATCCACAAC TGACTCACAC 
TTCCCCAGAC AGGACCAGTT GCCCTCATTT CCAAAGAACT GCACTCTGGA ATTAAAGGGA 
CTCTTCCATT TTGAAGAAGG CATCCAGAAG CTGTATCAGT GCAATGGGAT CGCCTGGAAA 
GCCTGGAGTC CCCAAACCAA GGATGTGGAA GACAAATCCT GTCCAGCCGG GTGGCACCAG 
CACTCAGGCT ACTGTCACAT CTTGATCACA GAGCAGAAAG GCACCTGGAA TGCGGCTGCC 
CAAGCTTGCA GGGAACAATA CCTGGGCAAC CTTGTAACTG TATTCTCCAG GCAGCACATG 
CGGTGGCTCT GGGACATTGG TGGGAGAAAG TCCTTTTGGA TAGGTTTGAA CGACCAAGTG 
CATGCTGGCC ACTGGGAGTG GATCGGTGGT GAACCTGTTG CCTTCACCAA TGGGAGAAGA 
GGGCCCTCTC AACGCTCCAA GCTTGGAAAG AGCTGTGTTT TGGTTCAAAG ACAAGGGAAA 
TGGCAAACAA AAGACTGTAG GAGAGCCAAA CCTCATAATT ATGTGTGTTC CAGAAAACTC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FREM1 ( XM_017014327.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016869816.1 Homo sapiens FRAS1 related extracellular matrix 1 (FREM1), transcript variant X12, mRNA.

Price & Availability↑

CloneID	OHu98463
Clone ID Related Accession (Same CDS sequence)	XM_017014327.1 , XM_017014327.2
Accession Version	XM_017014327.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5643bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	FRAS1-related extracellular matrix protein 1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017014327.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGTGT TTATTCTGGA AGTGGATCAG TTCATCCTGA CCTCCTTGAC TACATCAGTT 
CTGGACTGTG AAGAAGATGA GACCCCTAAA CCCTTGCTGG TGTTCAACAT TACTAAAGCC 
CCGCTCCAGG GCTATGTGAC TCACCTGTTG GATCACACCA GACCAATCTC CTCATTCACC 
TGGAAAGATC TCAGTGACAT GCAGATCGCC TATCAGCCAC CAAACAGCAG CCATTCTGAG 
AGGAGACATG ATGAGGTAGA ATTGGAGGTA TACGACTTCT TCTTTGAAAG GAGTGCACCT 
ATGACAGTCC ACATCTCCAT CAGAACAGCA GATACAAATG CCCCCCGTGT ATCCTGGAAT 
ACAGGTCTGA GTCTCCTTGA GGGGCAGTCT CGAGCCATCA CTTGGGAACA GTTTCAGGTT 
GTCGACAATG ACGACATTGG TGCTGTCCGG CTAGTCACCG TTGGTGGCCT GCAGCATGGA 
TGGCTGACTT TAAGAGGGGG GAAAGGGTTT CTCTTCACCG TGGCTGACCT CCAGGCTGGA 
GTTGTTCGCT ATCATCATGA TGACAGCGAC TCCACCAAAG ACTTCGTGGT CTTCCGGATA 
TTTGATGGCC ATCACAGCAT CCGTCACAAA TTCCCCATCA ACGTCTTGCC CAAAGATGAT 
AGTCCCCCGT TCCTCATAAC CAATGTTGTG ATTGAACTGG AGGAGGGGCA GACCATCCTG 
ATCCAGGGAT CCATGCTGCG AGCTTCAGAT GTGGACGCCA GTGATGACTA CATCTTCTTC 
AATATCACAA AGCCTCCACA GGCTGGGGAG ATCATGAAGA AGCCAGGGCC AGGACTGATA 
GGCTATCCTG TCCATGGCTT CCTTCAGAGG GATTTGTTTA ATGGAATCAT TTATTATCGT 
CATTTTGGTG GAGAAATCTT TGAAGATTCT TTTCAATTTG TCCTGTGGGA CAGCCATGAA 
CCTCCAAATC TTTCAGTGCC ACAGGTGGCA ACAATCCATA TAACTCCAGT GGATGACCAG 
CTTCCAAAAG AGGCTCCTGG AGTTTCTCGG CATTTGGTTG TCAAGGAAAC TGAGGTGGCC 
TATATAACTA AGAAACAGCT ACATTTTATA GATTCAGAAT CATATGACAG GGAGCTGGTC 
TACACAATAA CTACTCCTCC ATTTTTCTCC TTCAGCCACA GACACTTGGA TGCTGGGAAA 
TTATTTATGG TGGACAGCAT ACCAAAAGTA GTTAAGAATC CTACGGCCCT GGAGCTGAGG 
TCATTCACTC AGCATGCTGT GAACTATATG AAAGTGGCCT ACATGCCCCC CATGCAAGAC 
ATTGGTCCCC ATTGCAGAGA TGTCCAGTTC ACATTTTCTG TCAGTAACCA ACATGGCGGT 
ACTTTGCATG GGATCTGCTT TAACATTACA ATCCTCCCAG TGGACAATCA AGTTCCTGAG 
GCGTTCACCA ACCCTCTGAA AGTGACTGAG GGAGGTCAAA GCATCATCAG CACAGAGCAC 
ATTCTAATTT CTGATGCAGA TACCAAGCTG GACAATATTG ACCTCTCCCT GCGGGAATTG 
CCTCTGCACG GAAGGGTGGA GCTGAATGGA TTTCCTCTAA ATTCAGGGGG CACATTTTCT 
TGGGGCGATC TCCATACCTT AAAAGTTAGG TATCAACATG ATGGAACTGA AGTTCTTCAG 
GATGACCTAC TCTTGGAGGT CACCGATGGC ACAAATTCAG CAGAATTTGT ACTACATGTT 
GAGGTATTCC CTGTCAACGA TGAGCCACCA GTCTTAAAGG CTGACCTCAT GCCTGTCATG 
AATTGCTCAG AGGGAGGAGA GGTGGTCATC ACCTCTGAAT ACATTTTTGC TACTGATGTG 
GACAGCGATA ACTTGAAGTT GATGTTTGTG ATTGCTCGCG AACCTCAGCA TGGGGTGGTG 
AGGAGAGCTG GAGTCACAGT GGATCAGTTC TCTCAGAGAG ATGTTATCTC AGAGGCCGTG 
ACATACAAAC ACACAGGTGG CGAGATTGGC CTGATGCCTT GTTTTGACAC CATTACATTG 
GTGGTTTCGG ATGGAGAGGC TGGCCCTTTT GTGAATGGCT GTTGCTACAA TGGACCCAAT 
CCATCTGTTC CACTTCATGC GTCCTTTCCA GTGTATGATC TCAACATCAC GGTATACCCA 
GTAGACAACC AGCCACCTTC CATTGCAATA GGTCCCGTGT TTGTTGTAGA TGAGGGCTGC 
TCAACAGCCC TTACTGTTAA CCATTTGTCC GCCACTGACC CTGACACTGC AGCAGATGAC 
TTGGAATTTG TTTTGGTTTC TCCTCCTCAG TTTGGCTACC TCGAAAATAT ACTCCCTTCT 
GTGGGTTTTG AAAAAAGCAA TATTGGCATA AGTATAGATT CATTTCAGTG GAAAGACATG 
AACGCTTTTC ACATTAACTA TGTGCAGTCC AGGCATCTGA GGATAGAACC AACTGCCGAC 
CAGTTCACGG TGTACGTCAC AGATGGGAAG CATCACTCCT TGGAGATACC ATTTTCTATT 
ATAATCAACC CCACAAATGA TGAAGCTCCT GACTTTGTAG TGCAGAATAT TACTGTGTGT 
GAGGGTCAGA TGAAAGAGCT GGACTCTTCC ATCATCAGCG CTGTGGACCT GGACATTCCC 
CAGGATGCCC TGCTGTTCAG CATCACTCAA AAGCCACGCC ATGGCCTCCT CATCGATAGG 
GGGTTTAGCA AAGACTTCTC TGAGAATAAG CAGCCAGCCA ACCCTCACCA GAAACATGCA 
CCTGTTCACA GCTTTTCCAT GGAACTCCTC AAGACTGGAA TGAGGTTGAC GTACATGCAT 
GATGACTCAG AGAGCCTTGC TGATGATTTT ACAATCCAAT TGTCAGATGG GAAACATAAG 
ATACTTAAAA CCATTTCAGT AGAGGTCATC CCAGTTAATG ATGAAAAACC AATGCTGAGC 
AAGAAGGCTG AAATTGCAAT GAATATGGGT GAAACTCGTA TTATTTCCAG TGCTATTCTT 
TCAGCCATAG ATGAAGACTC ACCCAGGGAG AAGATTTACT ATGTATTTGA AAGGCTTCCC 
CAAAATGGGC AACTTCAGCT TAAGATAGGG AGGGACTGGG TTCCTCTCTC CCCTGGCATG 
AAATGCACTC AGGAGGAAGT GGATCTGAAC TTGCTGAGAT ACACACACAC CGGGGCAATG 
GATTCCCAGA ATCAAGATAG CTTCACCTTC TACCTTTGGG ATGGCAACAA CAGGTCCCCT 
GCTCTTGACT GTCAAATCAC CATCAAGGAC ATGGAAAAAG GTGATATTGT CATCCTTACA 
AAACCACTCG TGGTGTCTAA AGGTGATAGA GGTTTCTTAA CAACCACCAC GCTCCTGGCT 
GTGGACGGAA CAGACAAGCC TGAGGAACTG CTCTATGTCA TCACCTCCCC TCCGCGATAT 
GGCCAGATCG AATATGTTCA CTATCCTGGA GTTCCCATTA CAAACTTCAG CCAAATGGAT 
GTAGTGGGGC AGACAGTTTG CTATGTACAC AAGAGCAAGG TGACTGTCTC CAGTGACAGA 
TTCAGATTCA TCATCAGCAA TGGACTGCGG ACCGAGCACG GGGTGTTTGA GATCACACTG 
GAGACTGTGG ACAGAGCCCT GCCTGTGGTA ACCAGGAACA AGGGGTTGAG ACTGGCCCAA 
GGGGCCGTGG GCCTGCTTTC CCCTGACCTC CTTCAGCTGA CCGACCCTGA TACACCTGCG 
GAGAACCTCA CCTTCCTCTT GGTTCAGCTC CCCCAGCATG GCCAGCTCTA CCTGTGGGGG 
ACAGGGCTAC TTCAACACAA TTTCACCCAG CAGGATGTGG ACAGCAAGAA TGTGGCCTAT 
CGGCACTCAG GAGGGGACTC CCAGACTGAC TGCTTTACTT TCATGGCCAC AGATGGGACA 
AACCAAGGCT TTATTGTGAA TGGGAGAGTG TGGGAAGAAC CTGTTTTATT CACCATTCAG 
GTAGACCAAT TGGACAAAAC AGCTCCTCGT ATCACACTCT TGCATTCCCC TTCTCAAGTG 
GGGCTCCTGA AAAATGGCTG CTACGGGATT TACATCACTT CCCGCGTGTT GAAGGCATCA 
GACCCTGACA CTGAGGACGA TCAGATCATC TTTAAAATTC TACAAGGCCC AAAACATGGA 
CATCTGGAGA ACACAACAAC AGGTGAATTT ATCCATGAGA AATTTAGCCA AAAGGACTTA 
AACAGTAAGA CTATTCTTTA CATCATAAAC CCATCTTTGG AAGTAAATTC AGATACCGTG 
GAATTTCAAA TCATGGACCC CACAGGGAAC TCGGCCACTC CTCAAATTTT GGAACTGAAG 
TGGTCTCATA TTGAATGGTC ACAGACCGAA TATGAAGTCT GTGAGAATGT GGGTTTGTTG 
CCCTTGGAAA TTATCAGAAG GGGATATTCC ATGGACTCGG CCTTTGTGGG TATAAAGGTC 
AACCAAGTGT CAGCTGCAGT TGGAAAAGAT TTCACCGTGA TTCCATCTAA ACTGATTCAG 
TTTGACCCAG GAATGTCAAC TAAGATGTGG AATATAGCAA TTACCTATGA CGGATTAGAG 
GAAGATGATG AGGTCTTTGA AGTAATTCTG AACTCCCCTG TGAATGCAGT TCTTGGCACA 
AAGACAAAAG CTGCAGTGAA AATTTTGGAC TCAAAAGGAG GACAATGCCA TCCTTCATAT 
TCCTCCAACC AAAGCAAGCA CAGCACATGG GAGAAGGGCA TTTGGCATCT GCTGCCCCCA 
GGGTCTTCCT CATCCACCAC TTCTGGTTCC TTTCATCTGG AAAGAAGACC TCTTCCATCT 
TCCATGCAGC TAGCAGTCAT CAGGGGAGAC ACCCTGCGGG GCTTTGATTC TACAGATCTT 
TCTCAAAGGA AGCTTAGGAC CCGTGGGAAT GGCAAAACAG TTCGTCCATC CTCTGTTTAT 
AGAAATGGAA CAGACATCAT CTATAATTAT CATGGGATAG TTTCCTTGAA ACTGGAGGAT 
GACAGTTTCC CAACTCACAA AAGGAAGGCC AAAGTATCCA TCATTAGTCA GCCACAAAAG 
ACAATCAAAG TGGCAGAACT GCCTCAAGCA GATAAGGTGG AATCCACAAC TGACTCACAC 
TTCCCCAGAC AGGACCAGTT GCCCTCATTT CCAAAGAACT GCACTCTGGA ATTAAAGGGA 
CTCTTCCATT TTGAAGAAGG CATCCAGAAG CTGTATCAGT GCAATGGGAT CGCCTGGAAA 
GCCTGGAGTC CCCAAACCAA GGATGTGGAA GACAAATCCT GTCCAGCCGG GTGGCACCAG 
CACTCAGGCT ACTGTCACAT CTTGATCACA GAGCAGAAAG GCACCTGGAA TGCGGCTGCC 
CAAGCTTGCA GGGAACAATA CCTGGGCAAC CTTGTAACTG TATTCTCCAG GCAGCACATG 
CGGTGGCTCT GGGACATTGG TGGGAGAAAG TCCTTTTGGA TAGGTTTGAA CGACCAAGTG 
CATGCTGGCC ACTGGGAGTG GATCGGTGGT GAACCTGTTG CCTTCACCAA TGGGAGAAGA 
GGGCCCTCTC AACGCTCCAA GCTTGGAAAG AGCTGTGTTT TGGTTCAAAG ACAAGGGAAA 
TGGCAAACAA AAGACTGTAG GAGAGCCAAA CCTCATAATT ATGTGTGTTC CAGAAAACTC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016869816.1
CDS	150..5792
Translation	MAVFILEVDQFILTSLTTSVLDCEEDETPKPLLVFNITKAPLQGYVTHLLDHTRPISSFTWKDLSDMQIAYQPPNSSHSERRHDEVELEVYDFFFERSAPMTVHISIRTADTNAPRVSWNTGLSLLEGQSRAITWEQFQVVDNDDIGAVRLVTVGGLQHGWLTLRGGKGFLFTVADLQAGVVRYHHDDSDSTKDFVVFRIFDGHHSIRHKFPINVLPKDDSPPFLITNVVIELEEGQTILIQGSMLRASDVDASDDYIFFNITKPPQAGEIMKKPGPGLIGYPVHGFLQRDLFNGIIYYRHFGGEIFEDSFQFVLWDSHEPPNLSVPQVATIHITPVDDQLPKEAPGVSRHLVVKETEVAYITKKQLHFIDSESYDRELVYTITTPPFFSFSHRHLDAGKLFMVDSIPKVVKNPTALELRSFTQHAVNYMKVAYMPPMQDIGPHCRDVQFTFSVSNQHGGTLHGICFNITILPVDNQVPEAFTNPLKVTEGGQSIISTEHILISDADTKLDNIDLSLRELPLHGRVELNGFPLNSGGTFSWGDLHTLKVRYQHDGTEVLQDDLLLEVTDGTNSAEFVLHVEVFPVNDEPPVLKADLMPVMNCSEGGEVVITSEYIFATDVDSDNLKLMFVIAREPQHGVVRRAGVTVDQFSQRDVISEAVTYKHTGGEIGLMPCFDTITLVVSDGEAGPFVNGCCYNGPNPSVPLHASFPVYDLNITVYPVDNQPPSIAIGPVFVVDEGCSTALTVNHLSATDPDTAADDLEFVLVSPPQFGYLENILPSVGFEKSNIGISIDSFQWKDMNAFHINYVQSRHLRIEPTADQFTVYVTDGKHHSLEIPFSIIINPTNDEAPDFVVQNITVCEGQMKELDSSIISAVDLDIPQDALLFSITQKPRHGLLIDRGFSKDFSENKQPANPHQKHAPVHSFSMELLKTGMRLTYMHDDSESLADDFTIQLSDGKHKILKTISVEVIPVNDEKPMLSKKAEIAMNMGETRIISSAILSAIDEDSPREKIYYVFERLPQNGQLQLKIGRDWVPLSPGMKCTQEEVDLNLLRYTHTGAMDSQNQDSFTFYLWDGNNRSPALDCQITIKDMEKGDIVILTKPLVVSKGDRGFLTTTTLLAVDGTDKPEELLYVITSPPRYGQIEYVHYPGVPITNFSQMDVVGQTVCYVHKSKVTVSSDRFRFIISNGLRTEHGVFEITLETVDRALPVVTRNKGLRLAQGAVGLLSPDLLQLTDPDTPAENLTFLLVQLPQHGQLYLWGTGLLQHNFTQQDVDSKNVAYRHSGGDSQTDCFTFMATDGTNQGFIVNGRVWEEPVLFTIQVDQLDKTAPRITLLHSPSQVGLLKNGCYGIYITSRVLKASDPDTEDDQIIFKILQGPKHGHLENTTTGEFIHEKFSQKDLNSKTILYIINPSLEVNSDTVEFQIMDPTGNSATPQILELKWSHIEWSQTEYEVCENVGLLPLEIIRRGYSMDSAFVGIKVNQVSAAVGKDFTVIPSKLIQFDPGMSTKMWNIAITYDGLEEDDEVFEVILNSPVNAVLGTKTKAAVKILDSKGGQCHPSYSSNQSKHSTWEKGIWHLLPPGSSSSTTSGSFHLERRPLPSSMQLAVIRGDTLRGFDSTDLSQRKLRTRGNGKTVRPSSVYRNGTDIIYNYHGIVSLKLEDDSFPTHKRKAKVSIISQPQKTIKVAELPQADKVESTTDSHFPRQDQLPSFPKNCTLELKGLFHFEEGIQKLYQCNGIAWKAWSPQTKDVEDKSCPAGWHQHSGYCHILITEQKGTWNAAAQACREQYLGNLVTVFSRQHMRWLWDIGGRKSFWIGLNDQVHAGHWEWIGGEPVAFTNGRRGPSQRSKLGKSCVLVQRQGKWQTKDCRRAKPHNYVCSRKL

Target ORF information:

RefSeq Version	XM_017014327.2
Organism	Homo sapiens(human)
Definition	Homo sapiens FRAS1 related extracellular matrix 1 (FREM1), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017014327.2

ORF Insert Sequence:

ATGGCCGTGT TTATTCTGGA AGTGGATCAG TTCATCCTGA CCTCCTTGAC TACATCAGTT 
CTGGACTGTG AAGAAGATGA GACCCCTAAA CCCTTGCTGG TGTTCAACAT TACTAAAGCC 
CCGCTCCAGG GCTATGTGAC TCACCTGTTG GATCACACCA GACCAATCTC CTCATTCACC 
TGGAAAGATC TCAGTGACAT GCAGATCGCC TATCAGCCAC CAAACAGCAG CCATTCTGAG 
AGGAGACATG ATGAGGTAGA ATTGGAGGTA TACGACTTCT TCTTTGAAAG GAGTGCACCT 
ATGACAGTCC ACATCTCCAT CAGAACAGCA GATACAAATG CCCCCCGTGT ATCCTGGAAT 
ACAGGTCTGA GTCTCCTTGA GGGGCAGTCT CGAGCCATCA CTTGGGAACA GTTTCAGGTT 
GTCGACAATG ACGACATTGG TGCTGTCCGG CTAGTCACCG TTGGTGGCCT GCAGCATGGA 
TGGCTGACTT TAAGAGGGGG GAAAGGGTTT CTCTTCACCG TGGCTGACCT CCAGGCTGGA 
GTTGTTCGCT ATCATCATGA TGACAGCGAC TCCACCAAAG ACTTCGTGGT CTTCCGGATA 
TTTGATGGCC ATCACAGCAT CCGTCACAAA TTCCCCATCA ACGTCTTGCC CAAAGATGAT 
AGTCCCCCGT TCCTCATAAC CAATGTTGTG ATTGAACTGG AGGAGGGGCA GACCATCCTG 
ATCCAGGGAT CCATGCTGCG AGCTTCAGAT GTGGACGCCA GTGATGACTA CATCTTCTTC 
AATATCACAA AGCCTCCACA GGCTGGGGAG ATCATGAAGA AGCCAGGGCC AGGACTGATA 
GGCTATCCTG TCCATGGCTT CCTTCAGAGG GATTTGTTTA ATGGAATCAT TTATTATCGT 
CATTTTGGTG GAGAAATCTT TGAAGATTCT TTTCAATTTG TCCTGTGGGA CAGCCATGAA 
CCTCCAAATC TTTCAGTGCC ACAGGTGGCA ACAATCCATA TAACTCCAGT GGATGACCAG 
CTTCCAAAAG AGGCTCCTGG AGTTTCTCGG CATTTGGTTG TCAAGGAAAC TGAGGTGGCC 
TATATAACTA AGAAACAGCT ACATTTTATA GATTCAGAAT CATATGACAG GGAGCTGGTC 
TACACAATAA CTACTCCTCC ATTTTTCTCC TTCAGCCACA GACACTTGGA TGCTGGGAAA 
TTATTTATGG TGGACAGCAT ACCAAAAGTA GTTAAGAATC CTACGGCCCT GGAGCTGAGG 
TCATTCACTC AGCATGCTGT GAACTATATG AAAGTGGCCT ACATGCCCCC CATGCAAGAC 
ATTGGTCCCC ATTGCAGAGA TGTCCAGTTC ACATTTTCTG TCAGTAACCA ACATGGCGGT 
ACTTTGCATG GGATCTGCTT TAACATTACA ATCCTCCCAG TGGACAATCA AGTTCCTGAG 
GCGTTCACCA ACCCTCTGAA AGTGACTGAG GGAGGTCAAA GCATCATCAG CACAGAGCAC 
ATTCTAATTT CTGATGCAGA TACCAAGCTG GACAATATTG ACCTCTCCCT GCGGGAATTG 
CCTCTGCACG GAAGGGTGGA GCTGAATGGA TTTCCTCTAA ATTCAGGGGG CACATTTTCT 
TGGGGCGATC TCCATACCTT AAAAGTTAGG TATCAACATG ATGGAACTGA AGTTCTTCAG 
GATGACCTAC TCTTGGAGGT CACCGATGGC ACAAATTCAG CAGAATTTGT ACTACATGTT 
GAGGTATTCC CTGTCAACGA TGAGCCACCA GTCTTAAAGG CTGACCTCAT GCCTGTCATG 
AATTGCTCAG AGGGAGGAGA GGTGGTCATC ACCTCTGAAT ACATTTTTGC TACTGATGTG 
GACAGCGATA ACTTGAAGTT GATGTTTGTG ATTGCTCGCG AACCTCAGCA TGGGGTGGTG 
AGGAGAGCTG GAGTCACAGT GGATCAGTTC TCTCAGAGAG ATGTTATCTC AGAGGCCGTG 
ACATACAAAC ACACAGGTGG CGAGATTGGC CTGATGCCTT GTTTTGACAC CATTACATTG 
GTGGTTTCGG ATGGAGAGGC TGGCCCTTTT GTGAATGGCT GTTGCTACAA TGGACCCAAT 
CCATCTGTTC CACTTCATGC GTCCTTTCCA GTGTATGATC TCAACATCAC GGTATACCCA 
GTAGACAACC AGCCACCTTC CATTGCAATA GGTCCCGTGT TTGTTGTAGA TGAGGGCTGC 
TCAACAGCCC TTACTGTTAA CCATTTGTCC GCCACTGACC CTGACACTGC AGCAGATGAC 
TTGGAATTTG TTTTGGTTTC TCCTCCTCAG TTTGGCTACC TCGAAAATAT ACTCCCTTCT 
GTGGGTTTTG AAAAAAGCAA TATTGGCATA AGTATAGATT CATTTCAGTG GAAAGACATG 
AACGCTTTTC ACATTAACTA TGTGCAGTCC AGGCATCTGA GGATAGAACC AACTGCCGAC 
CAGTTCACGG TGTACGTCAC AGATGGGAAG CATCACTCCT TGGAGATACC ATTTTCTATT 
ATAATCAACC CCACAAATGA TGAAGCTCCT GACTTTGTAG TGCAGAATAT TACTGTGTGT 
GAGGGTCAGA TGAAAGAGCT GGACTCTTCC ATCATCAGCG CTGTGGACCT GGACATTCCC 
CAGGATGCCC TGCTGTTCAG CATCACTCAA AAGCCACGCC ATGGCCTCCT CATCGATAGG 
GGGTTTAGCA AAGACTTCTC TGAGAATAAG CAGCCAGCCA ACCCTCACCA GAAACATGCA 
CCTGTTCACA GCTTTTCCAT GGAACTCCTC AAGACTGGAA TGAGGTTGAC GTACATGCAT 
GATGACTCAG AGAGCCTTGC TGATGATTTT ACAATCCAAT TGTCAGATGG GAAACATAAG 
ATACTTAAAA CCATTTCAGT AGAGGTCATC CCAGTTAATG ATGAAAAACC AATGCTGAGC 
AAGAAGGCTG AAATTGCAAT GAATATGGGT GAAACTCGTA TTATTTCCAG TGCTATTCTT 
TCAGCCATAG ATGAAGACTC ACCCAGGGAG AAGATTTACT ATGTATTTGA AAGGCTTCCC 
CAAAATGGGC AACTTCAGCT TAAGATAGGG AGGGACTGGG TTCCTCTCTC CCCTGGCATG 
AAATGCACTC AGGAGGAAGT GGATCTGAAC TTGCTGAGAT ACACACACAC CGGGGCAATG 
GATTCCCAGA ATCAAGATAG CTTCACCTTC TACCTTTGGG ATGGCAACAA CAGGTCCCCT 
GCTCTTGACT GTCAAATCAC CATCAAGGAC ATGGAAAAAG GTGATATTGT CATCCTTACA 
AAACCACTCG TGGTGTCTAA AGGTGATAGA GGTTTCTTAA CAACCACCAC GCTCCTGGCT 
GTGGACGGAA CAGACAAGCC TGAGGAACTG CTCTATGTCA TCACCTCCCC TCCGCGATAT 
GGCCAGATCG AATATGTTCA CTATCCTGGA GTTCCCATTA CAAACTTCAG CCAAATGGAT 
GTAGTGGGGC AGACAGTTTG CTATGTACAC AAGAGCAAGG TGACTGTCTC CAGTGACAGA 
TTCAGATTCA TCATCAGCAA TGGACTGCGG ACCGAGCACG GGGTGTTTGA GATCACACTG 
GAGACTGTGG ACAGAGCCCT GCCTGTGGTA ACCAGGAACA AGGGGTTGAG ACTGGCCCAA 
GGGGCCGTGG GCCTGCTTTC CCCTGACCTC CTTCAGCTGA CCGACCCTGA TACACCTGCG 
GAGAACCTCA CCTTCCTCTT GGTTCAGCTC CCCCAGCATG GCCAGCTCTA CCTGTGGGGG 
ACAGGGCTAC TTCAACACAA TTTCACCCAG CAGGATGTGG ACAGCAAGAA TGTGGCCTAT 
CGGCACTCAG GAGGGGACTC CCAGACTGAC TGCTTTACTT TCATGGCCAC AGATGGGACA 
AACCAAGGCT TTATTGTGAA TGGGAGAGTG TGGGAAGAAC CTGTTTTATT CACCATTCAG 
GTAGACCAAT TGGACAAAAC AGCTCCTCGT ATCACACTCT TGCATTCCCC TTCTCAAGTG 
GGGCTCCTGA AAAATGGCTG CTACGGGATT TACATCACTT CCCGCGTGTT GAAGGCATCA 
GACCCTGACA CTGAGGACGA TCAGATCATC TTTAAAATTC TACAAGGCCC AAAACATGGA 
CATCTGGAGA ACACAACAAC AGGTGAATTT ATCCATGAGA AATTTAGCCA AAAGGACTTA 
AACAGTAAGA CTATTCTTTA CATCATAAAC CCATCTTTGG AAGTAAATTC AGATACCGTG 
GAATTTCAAA TCATGGACCC CACAGGGAAC TCGGCCACTC CTCAAATTTT GGAACTGAAG 
TGGTCTCATA TTGAATGGTC ACAGACCGAA TATGAAGTCT GTGAGAATGT GGGTTTGTTG 
CCCTTGGAAA TTATCAGAAG GGGATATTCC ATGGACTCGG CCTTTGTGGG TATAAAGGTC 
AACCAAGTGT CAGCTGCAGT TGGAAAAGAT TTCACCGTGA TTCCATCTAA ACTGATTCAG 
TTTGACCCAG GAATGTCAAC TAAGATGTGG AATATAGCAA TTACCTATGA CGGATTAGAG 
GAAGATGATG AGGTCTTTGA AGTAATTCTG AACTCCCCTG TGAATGCAGT TCTTGGCACA 
AAGACAAAAG CTGCAGTGAA AATTTTGGAC TCAAAAGGAG GACAATGCCA TCCTTCATAT 
TCCTCCAACC AAAGCAAGCA CAGCACATGG GAGAAGGGCA TTTGGCATCT GCTGCCCCCA 
GGGTCTTCCT CATCCACCAC TTCTGGTTCC TTTCATCTGG AAAGAAGACC TCTTCCATCT 
TCCATGCAGC TAGCAGTCAT CAGGGGAGAC ACCCTGCGGG GCTTTGATTC TACAGATCTT 
TCTCAAAGGA AGCTTAGGAC CCGTGGGAAT GGCAAAACAG TTCGTCCATC CTCTGTTTAT 
AGAAATGGAA CAGACATCAT CTATAATTAT CATGGGATAG TTTCCTTGAA ACTGGAGGAT 
GACAGTTTCC CAACTCACAA AAGGAAGGCC AAAGTATCCA TCATTAGTCA GCCACAAAAG 
ACAATCAAAG TGGCAGAACT GCCTCAAGCA GATAAGGTGG AATCCACAAC TGACTCACAC 
TTCCCCAGAC AGGACCAGTT GCCCTCATTT CCAAAGAACT GCACTCTGGA ATTAAAGGGA 
CTCTTCCATT TTGAAGAAGG CATCCAGAAG CTGTATCAGT GCAATGGGAT CGCCTGGAAA 
GCCTGGAGTC CCCAAACCAA GGATGTGGAA GACAAATCCT GTCCAGCCGG GTGGCACCAG 
CACTCAGGCT ACTGTCACAT CTTGATCACA GAGCAGAAAG GCACCTGGAA TGCGGCTGCC 
CAAGCTTGCA GGGAACAATA CCTGGGCAAC CTTGTAACTG TATTCTCCAG GCAGCACATG 
CGGTGGCTCT GGGACATTGG TGGGAGAAAG TCCTTTTGGA TAGGTTTGAA CGACCAAGTG 
CATGCTGGCC ACTGGGAGTG GATCGGTGGT GAACCTGTTG CCTTCACCAA TGGGAGAAGA 
GGGCCCTCTC AACGCTCCAA GCTTGGAAAG AGCTGTGTTT TGGTTCAAAG ACAAGGGAAA 
TGGCAAACAA AAGACTGTAG GAGAGCCAAA CCTCATAATT ATGTGTGTTC CAGAAAACTC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FREM1 ( XM_017014328.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016869817.1 Homo sapiens FRAS1 related extracellular matrix 1 (FREM1), transcript variant X14, mRNA.

Price & Availability↑

CloneID	OHu98464
Clone ID Related Accession (Same CDS sequence)	XM_017014328.1 , XM_017014328.2
Accession Version	XM_017014328.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5511bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	FRAS1-related extracellular matrix protein 1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACTCTC TGAGTTGGGG GGCTGCGAAT GCCGTGCTGC TGCTGCTCCT CCTGGCCTGG 
GCCAGCCCCA CCTTCATCAG CATCAACCGC GGGGTGAGGG TGATGAAGGG CCACTCTGCC 
TTCCTGTCAG GAGATGACCT GAAGTTTGCC ATCCCTAAAG AGAAAGATGC CTGCAAAGTG 
GAAGTTGTGA TGAATGAGCC AATAACCCAG AGGGTTGGGA AACTCACTCC ACAGGTCTTT 
GACTGCCATT TCCTTCCCAA CGAAGTCAAG TATGTTCACA ATGGTTGTCC AATTCTTGAT 
GAAGACACAG TGAAGCTCAG ACTTTACAGA TTTACTGAAA GAGATACCTT CATAGAAACT 
TTTATCCTGT GGGTCTATCT CCTGGAACCA GACTGTAACA TCATCCATAT GAGTAACAAT 
GTGCTGGAGG TGCCTGAATT CAATGGCTTG TCCCAAGCGA TTGATAAAAA TCTGCTCAGA 
TTCGATTATG ATAGGATGGC TAGCCTGGAA TGTACCGTCA GCCTGGACAC TGCGAGAACT 
CGGCTGCCAG CCCATGGCCA GATGGTTCTC GGGGAGCCTC GACCAGAAGA ACCTCGTGGA 
GATCAGCCAC ACAGTTTTTT CCCAGAGTCC CGTATGATGT CTTTTAATGC CTTTCTGGAA 
CTGAGAGCAA AACTGAAGTG TCCAGGTGGG AGCTGTACCC CAGGATTAAA GAAAATAGGA 
AGCCTCAAAG TGAGCTGTGA GGAGTTCCTG CTGATGGGCC TTCGTTATCA GCATCTGGAT 
CCCCCTTCAC CCAACATTGA TTATATCTCC ATCCAACTGG ACCTCACAGA TACCAGGAGC 
AAAATTGTGT ACAAGTCAGA GAGTGCGTGG CTGCCTGTCT ATATCAGAGC TGGAATTCCG 
AATCAGATTC CAAAGGCTGC ATTCATGGCC GTGTTTATTC TGGAAGTGGA TCAGTTCATC 
CTGACCTCCT TGACTACATC AGTTCTGGAC TGTGAAGAAG ATGAGACCCC TAAACCCTTG 
CTGGTGTTCA ACATTACTAA AGCCCCGCTC CAGGGCTATG TGACTCACCT GTTGGATCAC 
ACCAGACCAA TCTCCTCATT CACCTGGAAA GATCTCAGTG ACATGCAGAT CGCCTATCAG 
CCACCAAACA GCAGCCATTC TGAGAGGAGA CATGATGAGG TAGAATTGGA GGTATACGAC 
TTCTTCTTTG AAAGGAGTGC ACCTATGACA GTCCACATCT CCATCAGAAC AGCAGATACA 
AATGCCCCCC GTGTATCCTG GAATACAGGT CTGAGTCTCC TTGAGGGGCA GTCTCGAGCC 
ATCACTTGGG AACAGTTTCA GGTTGTCGAC AATGACGACA TTGGTGCTGT CCGGCTAGTC 
ACCGTTGGTG GCCTGCAGCA TGGATGGCTG ACTTTAAGAG GGGGGAAAGG GTTTCTCTTC 
ACCGTGGCTG ACCTCCAGGC TGGAGTTGTT CGCTATCATC ATGATGACAG CGACTCCACC 
AAAGACTTCG TGGTCTTCCG GATATTTGAT GGCCATCACA GCATCCGTCA CAAATTCCCC 
ATCAACGTCT TGCCCAAAGA TGATAGTCCC CCGTTCCTCA TAACCAATGT TGTGATTGAA 
CTGGAGGAGG GGCAGACCAT CCTGATCCAG GGATCCATGC TGCGAGCTTC AGATGTGGAC 
GCCAGTGATG ACTACATCTT CTTCAATATC ACAAAGCCTC CACAGGCTGG GGAGATCATG 
AAGAAGCCAG GGCCAGGACT GATAGGCTAT CCTGTCCATG GCTTCCTTCA GAGGGATTTG 
TTTAATGGAA TCATTTATTA TCGTCATTTT GGTGGAGAAA TCTTTGAAGA TTCTTTTCAA 
TTTGTCCTGT GGGACAGCCA TGAACCTCCA AATCTTTCAG TGCCACAGGT GGCAACAATC 
CATATAACTC CAGTGGATGA CCAGCTTCCA AAAGAGGCTC CTGGAGTTTC TCGGCATTTG 
GTTGTCAAGG AAACTGAGGT GGCCTATATA ACTAAGAAAC AGCTACATTT TATAGATTCA 
GAATCATATG ACAGGGAGCT GGTCTACACA ATAACTACTC CTCCATTTTT CTCCTTCAGC 
CACAGACACT TGGATGCTGG GAAATTATTT ATGGTGGACA GCATACCAAA AGTAGTTAAG 
AATCCTACGG CCCTGGAGCT GAGGTCATTC ACTCAGCATG CTGTGAACTA TATGAAAGTG 
GCCTACATGC CCCCCATGCA AGACATTGGT CCCCATTGCA GAGATGTCCA GTTCACATTT 
TCTGTCAGTA ACCAACATGG CGGTACTTTG CATGGGATCT GCTTTAACAT TACAATCCTC 
CCAGTGGACA ATCAAGTTCC TGAGGCGTTC ACCAACCCTC TGAAAGTGAC TGAGGGAGGT 
CAAAGCATCA TCAGCACAGA GCACATTCTA ATTTCTGATG CAGATACCAA GCTGGACAAT 
ATTGACCTCT CCCTGCGGGA ATTGCCTCTG CACGGAAGGG TGGAGCTGAA TGGATTTCCT 
CTAAATTCAG GGGGCACATT TTCTTGGGGC GATCTCCATA CCTTAAAAGT TAGGTATCAA 
CATGATGGAA CTGAAGTTCT TCAGGATGAC CTACTCTTGG AGGTCACCGA TGGCACAAAT 
TCAGCAGAAT TTGTACTACA TGTTGAGGTA TTCCCTGTCA ACGATGAGCC ACCAGTCTTA 
AAGGCTGACC TCATGCCTGT CATGAATTGC TCAGAGGGAG GAGAGGTGGT CATCACCTCT 
GAATACATTT TTGCTACTGA TGTGGACAGC GATAACTTGA AGTTGATGTT TGTGATTGCT 
CGCGAACCTC AGCATGGGGT GGTGAGGAGA GCTGGAGTCA CAGTGGATCA GTTCTCTCAG 
AGAGATGTTA TCTCAGAGGC CGTGACATAC AAACACACAG GTGGCGAGAT TGGCCTGATG 
CCTTGTTTTG ACACCATTAC ATTGGTGGTT TCGGATGGAG AGGCTGGCCC TTTTGTGAAT 
GGCTGTTGCT ACAATGGACC CAATCCATCT GTTCCACTTC ATGCGTCCTT TCCAGTGTAT 
GATCTCAACA TCACGGTATA CCCAGTAGAC AACCAGCCAC CTTCCATTGC AATAGGTCCC 
GTGTTTGTTG TAGATGAGGG CTGCTCAACA GCCCTTACTG TTAACCATTT GTCCGCCACT 
GACCCTGACA CTGCAGCAGA TGACTTGGAA TTTGTTTTGG TTTCTCCTCC TCAGTTTGGC 
TACCTCGAAA ATATACTCCC TTCTGTGGGT TTTGAAAAAA GCAATATTGG CATAAGTATA 
GATTCATTTC AGTGGAAAGA CATGAACGCT TTTCACATTA ACTATGTGCA GTCCAGGCAT 
CTGAGGATAG AACCAACTGC CGACCAGTTC ACGGTGTACG TCACAGATGG GAAGCATCAC 
TCCTTGGAGA TACCATTTTC TATTATAATC AACCCCACAA ATGATGAAGC TCCTGACTTT 
GTAGTGCAGA ATATTACTGT GTGTGAGGGT CAGATGAAAG AGCTGGACTC TTCCATCATC 
AGCGCTGTGG ACCTGGACAT TCCCCAGGAT GCCCTGCTGT TCAGCATCAC TCAAAAGCCA 
CGCCATGGCC TCCTCATCGA TAGGGGGTTT AGCAAAGACT TCTCTGAGAA TAAGCAGCCA 
GCCAACCCTC ACCAGAAACA TGCACCTGTT CACAGCTTTT CCATGGAACT CCTCAAGACT 
GGAATGAGGT TGACGTACAT GCATGATGAC TCAGAGAGCC TTGCTGATGA TTTTACAATC 
CAATTGTCAG ATGGGAAACA TAAGATACTT AAAACCATTT CAGTAGAGGT CATCCCAGTT 
AATGATGAAA AACCAATGCT GAGCAAGAAG GCTGAAATTG CAATGAATAT GGGTGAAACT 
CGTATTATTT CCAGTGCTAT TCTTTCAGCC ATAGATGAAG ACTCACCCAG GGAGAAGATT 
TACTATGTAT TTGAAAGGCT TCCCCAAAAT GGGCAACTTC AGCTTAAGAT AGGGAGGGAC 
TGGGTTCCTC TCTCCCCTGG CATGAAATGC ACTCAGGAGG AAGTGGATCT GAACTTGCTG 
AGATACACAC ACACCGGGGC AATGGATTCC CAGAATCAAG ATAGCTTCAC CTTCTACCTT 
TGGGATGGCA ACAACAGGTC CCCTGCTCTT GACTGTCAAA TCACCATCAA GGACATGGAA 
AAAGGTGATA TTGTCATCCT TACAAAACCA CTCGTGGTGT CTAAAGGTGA TAGAGGTTTC 
TTAACAACCA CCACGCTCCT GGCTGTGGAC GGAACAGACA AGCCTGAGGA ACTGCTCTAT 
GTCATCACCT CCCCTCCGCG ATATGGCCAG ATCGAATATG TTCACTATCC TGGAGTTCCC 
ATTACAAACT TCAGCCAAAT GGATGTAGTG GGGCAGACAG TTTGCTATGT ACACAAGAGC 
AAGGTGACTG TCTCCAGTGA CAGATTCAGA TTCATCATCA GCAATGGACT GCGGACCGAG 
CACGGGGTGT TTGAGATCAC ACTGGAGACT GTGGACAGAG CCCTGCCTGT GGTAACCAGG 
AACAAGGGGT TGAGACTGGC CCAAGGGGCC GTGGGCCTGC TTTCCCCTGA CCTCCTTCAG 
CTGACCGACC CTGATACACC TGCGGAGAAC CTCACCTTCC TCTTGGTTCA GCTCCCCCAG 
CATGGCCAGC TCTACCTGTG GGGGACAGGG CTACTTCAAC ACAATTTCAC CCAGCAGGAT 
GTGGACAGCA AGAATGTGGC CTATCGGCAC TCAGGAGGGG ACTCCCAGAC TGACTGCTTT 
ACTTTCATGG CCACAGATGG GACAAACCAA GGCTTTATTG TGAATGGGAG AGTGTGGGAA 
GAACCTGTTT TATTCACCAT TCAGGTAGAC CAATTGGACA AAACAGCTCC TCGTATCACA 
CTCTTGCATT CCCCTTCTCA AGTGGGGCTC CTGAAAAATG GCTGCTACGG GATTTACATC 
ACTTCCCGCG TGTTGAAGGC ATCAGACCCT GACACTGAGG ACGATCAGAT CATCTTTAAA 
ATTCTACAAG GCCCAAAACA TGGACATCTG GAGAACACAA CAACAGGTGA ATTTATCCAT 
GAGAAATTTA GCCAAAAGGA CTTAAACAGT AAGACTATTC TTTACATCAT AAACCCATCT 
TTGGAAGTAA ATTCAGATAC CGTGGAATTT CAAATCATGG ACCCCACAGG GAACTCGGCC 
ACTCCTCAAA TTTTGGAACT GAAGTGGTCT CATATTGAAT GGTCACAGAC CGAATATGAA 
GTCTGTGAGA ATGTGGGTTT GTTGCCCTTG GAAATTATCA GAAGGGGATA TTCCATGGAC 
TCGGCCTTTG TGGGTATAAA GGTCAACCAA GTGTCAGCTG CAGTTGGAAA AGATTTCACC 
GTGATTCCAT CTAAACTGAT TCAGTTTGAC CCAGGTGCAA GTGGAAATAA AAAAGGCTGG 
TACTTGCTCT CAAGTGTGTT CCATTGTGTG AGAAACACAG ACAAGAATTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016869817.1
CDS	1065..6575
Translation	MNSLSWGAANAVLLLLLLAWASPTFISINRGVRVMKGHSAFLSGDDLKFAIPKEKDACKVEVVMNEPITQRVGKLTPQVFDCHFLPNEVKYVHNGCPILDEDTVKLRLYRFTERDTFIETFILWVYLLEPDCNIIHMSNNVLEVPEFNGLSQAIDKNLLRFDYDRMASLECTVSLDTARTRLPAHGQMVLGEPRPEEPRGDQPHSFFPESRMMSFNAFLELRAKLKCPGGSCTPGLKKIGSLKVSCEEFLLMGLRYQHLDPPSPNIDYISIQLDLTDTRSKIVYKSESAWLPVYIRAGIPNQIPKAAFMAVFILEVDQFILTSLTTSVLDCEEDETPKPLLVFNITKAPLQGYVTHLLDHTRPISSFTWKDLSDMQIAYQPPNSSHSERRHDEVELEVYDFFFERSAPMTVHISIRTADTNAPRVSWNTGLSLLEGQSRAITWEQFQVVDNDDIGAVRLVTVGGLQHGWLTLRGGKGFLFTVADLQAGVVRYHHDDSDSTKDFVVFRIFDGHHSIRHKFPINVLPKDDSPPFLITNVVIELEEGQTILIQGSMLRASDVDASDDYIFFNITKPPQAGEIMKKPGPGLIGYPVHGFLQRDLFNGIIYYRHFGGEIFEDSFQFVLWDSHEPPNLSVPQVATIHITPVDDQLPKEAPGVSRHLVVKETEVAYITKKQLHFIDSESYDRELVYTITTPPFFSFSHRHLDAGKLFMVDSIPKVVKNPTALELRSFTQHAVNYMKVAYMPPMQDIGPHCRDVQFTFSVSNQHGGTLHGICFNITILPVDNQVPEAFTNPLKVTEGGQSIISTEHILISDADTKLDNIDLSLRELPLHGRVELNGFPLNSGGTFSWGDLHTLKVRYQHDGTEVLQDDLLLEVTDGTNSAEFVLHVEVFPVNDEPPVLKADLMPVMNCSEGGEVVITSEYIFATDVDSDNLKLMFVIAREPQHGVVRRAGVTVDQFSQRDVISEAVTYKHTGGEIGLMPCFDTITLVVSDGEAGPFVNGCCYNGPNPSVPLHASFPVYDLNITVYPVDNQPPSIAIGPVFVVDEGCSTALTVNHLSATDPDTAADDLEFVLVSPPQFGYLENILPSVGFEKSNIGISIDSFQWKDMNAFHINYVQSRHLRIEPTADQFTVYVTDGKHHSLEIPFSIIINPTNDEAPDFVVQNITVCEGQMKELDSSIISAVDLDIPQDALLFSITQKPRHGLLIDRGFSKDFSENKQPANPHQKHAPVHSFSMELLKTGMRLTYMHDDSESLADDFTIQLSDGKHKILKTISVEVIPVNDEKPMLSKKAEIAMNMGETRIISSAILSAIDEDSPREKIYYVFERLPQNGQLQLKIGRDWVPLSPGMKCTQEEVDLNLLRYTHTGAMDSQNQDSFTFYLWDGNNRSPALDCQITIKDMEKGDIVILTKPLVVSKGDRGFLTTTTLLAVDGTDKPEELLYVITSPPRYGQIEYVHYPGVPITNFSQMDVVGQTVCYVHKSKVTVSSDRFRFIISNGLRTEHGVFEITLETVDRALPVVTRNKGLRLAQGAVGLLSPDLLQLTDPDTPAENLTFLLVQLPQHGQLYLWGTGLLQHNFTQQDVDSKNVAYRHSGGDSQTDCFTFMATDGTNQGFIVNGRVWEEPVLFTIQVDQLDKTAPRITLLHSPSQVGLLKNGCYGIYITSRVLKASDPDTEDDQIIFKILQGPKHGHLENTTTGEFIHEKFSQKDLNSKTILYIINPSLEVNSDTVEFQIMDPTGNSATPQILELKWSHIEWSQTEYEVCENVGLLPLEIIRRGYSMDSAFVGIKVNQVSAAVGKDFTVIPSKLIQFDPGASGNKKGWYLLSSVFHCVRNTDKN

Target ORF information:

RefSeq Version	XM_017014328.1
Organism	Homo sapiens(human)
Definition	Homo sapiens FRAS1 related extracellular matrix 1 (FREM1), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017014328.1

ORF Insert Sequence:

ATGAACTCTC TGAGTTGGGG GGCTGCGAAT GCCGTGCTGC TGCTGCTCCT CCTGGCCTGG 
GCCAGCCCCA CCTTCATCAG CATCAACCGC GGGGTGAGGG TGATGAAGGG CCACTCTGCC 
TTCCTGTCAG GAGATGACCT GAAGTTTGCC ATCCCTAAAG AGAAAGATGC CTGCAAAGTG 
GAAGTTGTGA TGAATGAGCC AATAACCCAG AGGGTTGGGA AACTCACTCC ACAGGTCTTT 
GACTGCCATT TCCTTCCCAA CGAAGTCAAG TATGTTCACA ATGGTTGTCC AATTCTTGAT 
GAAGACACAG TGAAGCTCAG ACTTTACAGA TTTACTGAAA GAGATACCTT CATAGAAACT 
TTTATCCTGT GGGTCTATCT CCTGGAACCA GACTGTAACA TCATCCATAT GAGTAACAAT 
GTGCTGGAGG TGCCTGAATT CAATGGCTTG TCCCAAGCGA TTGATAAAAA TCTGCTCAGA 
TTCGATTATG ATAGGATGGC TAGCCTGGAA TGTACCGTCA GCCTGGACAC TGCGAGAACT 
CGGCTGCCAG CCCATGGCCA GATGGTTCTC GGGGAGCCTC GACCAGAAGA ACCTCGTGGA 
GATCAGCCAC ACAGTTTTTT CCCAGAGTCC CGTATGATGT CTTTTAATGC CTTTCTGGAA 
CTGAGAGCAA AACTGAAGTG TCCAGGTGGG AGCTGTACCC CAGGATTAAA GAAAATAGGA 
AGCCTCAAAG TGAGCTGTGA GGAGTTCCTG CTGATGGGCC TTCGTTATCA GCATCTGGAT 
CCCCCTTCAC CCAACATTGA TTATATCTCC ATCCAACTGG ACCTCACAGA TACCAGGAGC 
AAAATTGTGT ACAAGTCAGA GAGTGCGTGG CTGCCTGTCT ATATCAGAGC TGGAATTCCG 
AATCAGATTC CAAAGGCTGC ATTCATGGCC GTGTTTATTC TGGAAGTGGA TCAGTTCATC 
CTGACCTCCT TGACTACATC AGTTCTGGAC TGTGAAGAAG ATGAGACCCC TAAACCCTTG 
CTGGTGTTCA ACATTACTAA AGCCCCGCTC CAGGGCTATG TGACTCACCT GTTGGATCAC 
ACCAGACCAA TCTCCTCATT CACCTGGAAA GATCTCAGTG ACATGCAGAT CGCCTATCAG 
CCACCAAACA GCAGCCATTC TGAGAGGAGA CATGATGAGG TAGAATTGGA GGTATACGAC 
TTCTTCTTTG AAAGGAGTGC ACCTATGACA GTCCACATCT CCATCAGAAC AGCAGATACA 
AATGCCCCCC GTGTATCCTG GAATACAGGT CTGAGTCTCC TTGAGGGGCA GTCTCGAGCC 
ATCACTTGGG AACAGTTTCA GGTTGTCGAC AATGACGACA TTGGTGCTGT CCGGCTAGTC 
ACCGTTGGTG GCCTGCAGCA TGGATGGCTG ACTTTAAGAG GGGGGAAAGG GTTTCTCTTC 
ACCGTGGCTG ACCTCCAGGC TGGAGTTGTT CGCTATCATC ATGATGACAG CGACTCCACC 
AAAGACTTCG TGGTCTTCCG GATATTTGAT GGCCATCACA GCATCCGTCA CAAATTCCCC 
ATCAACGTCT TGCCCAAAGA TGATAGTCCC CCGTTCCTCA TAACCAATGT TGTGATTGAA 
CTGGAGGAGG GGCAGACCAT CCTGATCCAG GGATCCATGC TGCGAGCTTC AGATGTGGAC 
GCCAGTGATG ACTACATCTT CTTCAATATC ACAAAGCCTC CACAGGCTGG GGAGATCATG 
AAGAAGCCAG GGCCAGGACT GATAGGCTAT CCTGTCCATG GCTTCCTTCA GAGGGATTTG 
TTTAATGGAA TCATTTATTA TCGTCATTTT GGTGGAGAAA TCTTTGAAGA TTCTTTTCAA 
TTTGTCCTGT GGGACAGCCA TGAACCTCCA AATCTTTCAG TGCCACAGGT GGCAACAATC 
CATATAACTC CAGTGGATGA CCAGCTTCCA AAAGAGGCTC CTGGAGTTTC TCGGCATTTG 
GTTGTCAAGG AAACTGAGGT GGCCTATATA ACTAAGAAAC AGCTACATTT TATAGATTCA 
GAATCATATG ACAGGGAGCT GGTCTACACA ATAACTACTC CTCCATTTTT CTCCTTCAGC 
CACAGACACT TGGATGCTGG GAAATTATTT ATGGTGGACA GCATACCAAA AGTAGTTAAG 
AATCCTACGG CCCTGGAGCT GAGGTCATTC ACTCAGCATG CTGTGAACTA TATGAAAGTG 
GCCTACATGC CCCCCATGCA AGACATTGGT CCCCATTGCA GAGATGTCCA GTTCACATTT 
TCTGTCAGTA ACCAACATGG CGGTACTTTG CATGGGATCT GCTTTAACAT TACAATCCTC 
CCAGTGGACA ATCAAGTTCC TGAGGCGTTC ACCAACCCTC TGAAAGTGAC TGAGGGAGGT 
CAAAGCATCA TCAGCACAGA GCACATTCTA ATTTCTGATG CAGATACCAA GCTGGACAAT 
ATTGACCTCT CCCTGCGGGA ATTGCCTCTG CACGGAAGGG TGGAGCTGAA TGGATTTCCT 
CTAAATTCAG GGGGCACATT TTCTTGGGGC GATCTCCATA CCTTAAAAGT TAGGTATCAA 
CATGATGGAA CTGAAGTTCT TCAGGATGAC CTACTCTTGG AGGTCACCGA TGGCACAAAT 
TCAGCAGAAT TTGTACTACA TGTTGAGGTA TTCCCTGTCA ACGATGAGCC ACCAGTCTTA 
AAGGCTGACC TCATGCCTGT CATGAATTGC TCAGAGGGAG GAGAGGTGGT CATCACCTCT 
GAATACATTT TTGCTACTGA TGTGGACAGC GATAACTTGA AGTTGATGTT TGTGATTGCT 
CGCGAACCTC AGCATGGGGT GGTGAGGAGA GCTGGAGTCA CAGTGGATCA GTTCTCTCAG 
AGAGATGTTA TCTCAGAGGC CGTGACATAC AAACACACAG GTGGCGAGAT TGGCCTGATG 
CCTTGTTTTG ACACCATTAC ATTGGTGGTT TCGGATGGAG AGGCTGGCCC TTTTGTGAAT 
GGCTGTTGCT ACAATGGACC CAATCCATCT GTTCCACTTC ATGCGTCCTT TCCAGTGTAT 
GATCTCAACA TCACGGTATA CCCAGTAGAC AACCAGCCAC CTTCCATTGC AATAGGTCCC 
GTGTTTGTTG TAGATGAGGG CTGCTCAACA GCCCTTACTG TTAACCATTT GTCCGCCACT 
GACCCTGACA CTGCAGCAGA TGACTTGGAA TTTGTTTTGG TTTCTCCTCC TCAGTTTGGC 
TACCTCGAAA ATATACTCCC TTCTGTGGGT TTTGAAAAAA GCAATATTGG CATAAGTATA 
GATTCATTTC AGTGGAAAGA CATGAACGCT TTTCACATTA ACTATGTGCA GTCCAGGCAT 
CTGAGGATAG AACCAACTGC CGACCAGTTC ACGGTGTACG TCACAGATGG GAAGCATCAC 
TCCTTGGAGA TACCATTTTC TATTATAATC AACCCCACAA ATGATGAAGC TCCTGACTTT 
GTAGTGCAGA ATATTACTGT GTGTGAGGGT CAGATGAAAG AGCTGGACTC TTCCATCATC 
AGCGCTGTGG ACCTGGACAT TCCCCAGGAT GCCCTGCTGT TCAGCATCAC TCAAAAGCCA 
CGCCATGGCC TCCTCATCGA TAGGGGGTTT AGCAAAGACT TCTCTGAGAA TAAGCAGCCA 
GCCAACCCTC ACCAGAAACA TGCACCTGTT CACAGCTTTT CCATGGAACT CCTCAAGACT 
GGAATGAGGT TGACGTACAT GCATGATGAC TCAGAGAGCC TTGCTGATGA TTTTACAATC 
CAATTGTCAG ATGGGAAACA TAAGATACTT AAAACCATTT CAGTAGAGGT CATCCCAGTT 
AATGATGAAA AACCAATGCT GAGCAAGAAG GCTGAAATTG CAATGAATAT GGGTGAAACT 
CGTATTATTT CCAGTGCTAT TCTTTCAGCC ATAGATGAAG ACTCACCCAG GGAGAAGATT 
TACTATGTAT TTGAAAGGCT TCCCCAAAAT GGGCAACTTC AGCTTAAGAT AGGGAGGGAC 
TGGGTTCCTC TCTCCCCTGG CATGAAATGC ACTCAGGAGG AAGTGGATCT GAACTTGCTG 
AGATACACAC ACACCGGGGC AATGGATTCC CAGAATCAAG ATAGCTTCAC CTTCTACCTT 
TGGGATGGCA ACAACAGGTC CCCTGCTCTT GACTGTCAAA TCACCATCAA GGACATGGAA 
AAAGGTGATA TTGTCATCCT TACAAAACCA CTCGTGGTGT CTAAAGGTGA TAGAGGTTTC 
TTAACAACCA CCACGCTCCT GGCTGTGGAC GGAACAGACA AGCCTGAGGA ACTGCTCTAT 
GTCATCACCT CCCCTCCGCG ATATGGCCAG ATCGAATATG TTCACTATCC TGGAGTTCCC 
ATTACAAACT TCAGCCAAAT GGATGTAGTG GGGCAGACAG TTTGCTATGT ACACAAGAGC 
AAGGTGACTG TCTCCAGTGA CAGATTCAGA TTCATCATCA GCAATGGACT GCGGACCGAG 
CACGGGGTGT TTGAGATCAC ACTGGAGACT GTGGACAGAG CCCTGCCTGT GGTAACCAGG 
AACAAGGGGT TGAGACTGGC CCAAGGGGCC GTGGGCCTGC TTTCCCCTGA CCTCCTTCAG 
CTGACCGACC CTGATACACC TGCGGAGAAC CTCACCTTCC TCTTGGTTCA GCTCCCCCAG 
CATGGCCAGC TCTACCTGTG GGGGACAGGG CTACTTCAAC ACAATTTCAC CCAGCAGGAT 
GTGGACAGCA AGAATGTGGC CTATCGGCAC TCAGGAGGGG ACTCCCAGAC TGACTGCTTT 
ACTTTCATGG CCACAGATGG GACAAACCAA GGCTTTATTG TGAATGGGAG AGTGTGGGAA 
GAACCTGTTT TATTCACCAT TCAGGTAGAC CAATTGGACA AAACAGCTCC TCGTATCACA 
CTCTTGCATT CCCCTTCTCA AGTGGGGCTC CTGAAAAATG GCTGCTACGG GATTTACATC 
ACTTCCCGCG TGTTGAAGGC ATCAGACCCT GACACTGAGG ACGATCAGAT CATCTTTAAA 
ATTCTACAAG GCCCAAAACA TGGACATCTG GAGAACACAA CAACAGGTGA ATTTATCCAT 
GAGAAATTTA GCCAAAAGGA CTTAAACAGT AAGACTATTC TTTACATCAT AAACCCATCT 
TTGGAAGTAA ATTCAGATAC CGTGGAATTT CAAATCATGG ACCCCACAGG GAACTCGGCC 
ACTCCTCAAA TTTTGGAACT GAAGTGGTCT CATATTGAAT GGTCACAGAC CGAATATGAA 
GTCTGTGAGA ATGTGGGTTT GTTGCCCTTG GAAATTATCA GAAGGGGATA TTCCATGGAC 
TCGGCCTTTG TGGGTATAAA GGTCAACCAA GTGTCAGCTG CAGTTGGAAA AGATTTCACC 
GTGATTCCAT CTAAACTGAT TCAGTTTGAC CCAGGTGCAA GTGGAAATAA AAAAGGCTGG 
TACTTGCTCT CAAGTGTGTT CCATTGTGTG AGAAACACAG ACAAGAATTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FREM1 ( XM_017014328.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016869817.1 Homo sapiens FRAS1 related extracellular matrix 1 (FREM1), transcript variant X13, mRNA.

Price & Availability↑

CloneID	OHu98464
Clone ID Related Accession (Same CDS sequence)	XM_017014328.1 , XM_017014328.2
Accession Version	XM_017014328.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5511bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	FRAS1-related extracellular matrix protein 1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017014328.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACTCTC TGAGTTGGGG GGCTGCGAAT GCCGTGCTGC TGCTGCTCCT CCTGGCCTGG 
GCCAGCCCCA CCTTCATCAG CATCAACCGC GGGGTGAGGG TGATGAAGGG CCACTCTGCC 
TTCCTGTCAG GAGATGACCT GAAGTTTGCC ATCCCTAAAG AGAAAGATGC CTGCAAAGTG 
GAAGTTGTGA TGAATGAGCC AATAACCCAG AGGGTTGGGA AACTCACTCC ACAGGTCTTT 
GACTGCCATT TCCTTCCCAA CGAAGTCAAG TATGTTCACA ATGGTTGTCC AATTCTTGAT 
GAAGACACAG TGAAGCTCAG ACTTTACAGA TTTACTGAAA GAGATACCTT CATAGAAACT 
TTTATCCTGT GGGTCTATCT CCTGGAACCA GACTGTAACA TCATCCATAT GAGTAACAAT 
GTGCTGGAGG TGCCTGAATT CAATGGCTTG TCCCAAGCGA TTGATAAAAA TCTGCTCAGA 
TTCGATTATG ATAGGATGGC TAGCCTGGAA TGTACCGTCA GCCTGGACAC TGCGAGAACT 
CGGCTGCCAG CCCATGGCCA GATGGTTCTC GGGGAGCCTC GACCAGAAGA ACCTCGTGGA 
GATCAGCCAC ACAGTTTTTT CCCAGAGTCC CGTATGATGT CTTTTAATGC CTTTCTGGAA 
CTGAGAGCAA AACTGAAGTG TCCAGGTGGG AGCTGTACCC CAGGATTAAA GAAAATAGGA 
AGCCTCAAAG TGAGCTGTGA GGAGTTCCTG CTGATGGGCC TTCGTTATCA GCATCTGGAT 
CCCCCTTCAC CCAACATTGA TTATATCTCC ATCCAACTGG ACCTCACAGA TACCAGGAGC 
AAAATTGTGT ACAAGTCAGA GAGTGCGTGG CTGCCTGTCT ATATCAGAGC TGGAATTCCG 
AATCAGATTC CAAAGGCTGC ATTCATGGCC GTGTTTATTC TGGAAGTGGA TCAGTTCATC 
CTGACCTCCT TGACTACATC AGTTCTGGAC TGTGAAGAAG ATGAGACCCC TAAACCCTTG 
CTGGTGTTCA ACATTACTAA AGCCCCGCTC CAGGGCTATG TGACTCACCT GTTGGATCAC 
ACCAGACCAA TCTCCTCATT CACCTGGAAA GATCTCAGTG ACATGCAGAT CGCCTATCAG 
CCACCAAACA GCAGCCATTC TGAGAGGAGA CATGATGAGG TAGAATTGGA GGTATACGAC 
TTCTTCTTTG AAAGGAGTGC ACCTATGACA GTCCACATCT CCATCAGAAC AGCAGATACA 
AATGCCCCCC GTGTATCCTG GAATACAGGT CTGAGTCTCC TTGAGGGGCA GTCTCGAGCC 
ATCACTTGGG AACAGTTTCA GGTTGTCGAC AATGACGACA TTGGTGCTGT CCGGCTAGTC 
ACCGTTGGTG GCCTGCAGCA TGGATGGCTG ACTTTAAGAG GGGGGAAAGG GTTTCTCTTC 
ACCGTGGCTG ACCTCCAGGC TGGAGTTGTT CGCTATCATC ATGATGACAG CGACTCCACC 
AAAGACTTCG TGGTCTTCCG GATATTTGAT GGCCATCACA GCATCCGTCA CAAATTCCCC 
ATCAACGTCT TGCCCAAAGA TGATAGTCCC CCGTTCCTCA TAACCAATGT TGTGATTGAA 
CTGGAGGAGG GGCAGACCAT CCTGATCCAG GGATCCATGC TGCGAGCTTC AGATGTGGAC 
GCCAGTGATG ACTACATCTT CTTCAATATC ACAAAGCCTC CACAGGCTGG GGAGATCATG 
AAGAAGCCAG GGCCAGGACT GATAGGCTAT CCTGTCCATG GCTTCCTTCA GAGGGATTTG 
TTTAATGGAA TCATTTATTA TCGTCATTTT GGTGGAGAAA TCTTTGAAGA TTCTTTTCAA 
TTTGTCCTGT GGGACAGCCA TGAACCTCCA AATCTTTCAG TGCCACAGGT GGCAACAATC 
CATATAACTC CAGTGGATGA CCAGCTTCCA AAAGAGGCTC CTGGAGTTTC TCGGCATTTG 
GTTGTCAAGG AAACTGAGGT GGCCTATATA ACTAAGAAAC AGCTACATTT TATAGATTCA 
GAATCATATG ACAGGGAGCT GGTCTACACA ATAACTACTC CTCCATTTTT CTCCTTCAGC 
CACAGACACT TGGATGCTGG GAAATTATTT ATGGTGGACA GCATACCAAA AGTAGTTAAG 
AATCCTACGG CCCTGGAGCT GAGGTCATTC ACTCAGCATG CTGTGAACTA TATGAAAGTG 
GCCTACATGC CCCCCATGCA AGACATTGGT CCCCATTGCA GAGATGTCCA GTTCACATTT 
TCTGTCAGTA ACCAACATGG CGGTACTTTG CATGGGATCT GCTTTAACAT TACAATCCTC 
CCAGTGGACA ATCAAGTTCC TGAGGCGTTC ACCAACCCTC TGAAAGTGAC TGAGGGAGGT 
CAAAGCATCA TCAGCACAGA GCACATTCTA ATTTCTGATG CAGATACCAA GCTGGACAAT 
ATTGACCTCT CCCTGCGGGA ATTGCCTCTG CACGGAAGGG TGGAGCTGAA TGGATTTCCT 
CTAAATTCAG GGGGCACATT TTCTTGGGGC GATCTCCATA CCTTAAAAGT TAGGTATCAA 
CATGATGGAA CTGAAGTTCT TCAGGATGAC CTACTCTTGG AGGTCACCGA TGGCACAAAT 
TCAGCAGAAT TTGTACTACA TGTTGAGGTA TTCCCTGTCA ACGATGAGCC ACCAGTCTTA 
AAGGCTGACC TCATGCCTGT CATGAATTGC TCAGAGGGAG GAGAGGTGGT CATCACCTCT 
GAATACATTT TTGCTACTGA TGTGGACAGC GATAACTTGA AGTTGATGTT TGTGATTGCT 
CGCGAACCTC AGCATGGGGT GGTGAGGAGA GCTGGAGTCA CAGTGGATCA GTTCTCTCAG 
AGAGATGTTA TCTCAGAGGC CGTGACATAC AAACACACAG GTGGCGAGAT TGGCCTGATG 
CCTTGTTTTG ACACCATTAC ATTGGTGGTT TCGGATGGAG AGGCTGGCCC TTTTGTGAAT 
GGCTGTTGCT ACAATGGACC CAATCCATCT GTTCCACTTC ATGCGTCCTT TCCAGTGTAT 
GATCTCAACA TCACGGTATA CCCAGTAGAC AACCAGCCAC CTTCCATTGC AATAGGTCCC 
GTGTTTGTTG TAGATGAGGG CTGCTCAACA GCCCTTACTG TTAACCATTT GTCCGCCACT 
GACCCTGACA CTGCAGCAGA TGACTTGGAA TTTGTTTTGG TTTCTCCTCC TCAGTTTGGC 
TACCTCGAAA ATATACTCCC TTCTGTGGGT TTTGAAAAAA GCAATATTGG CATAAGTATA 
GATTCATTTC AGTGGAAAGA CATGAACGCT TTTCACATTA ACTATGTGCA GTCCAGGCAT 
CTGAGGATAG AACCAACTGC CGACCAGTTC ACGGTGTACG TCACAGATGG GAAGCATCAC 
TCCTTGGAGA TACCATTTTC TATTATAATC AACCCCACAA ATGATGAAGC TCCTGACTTT 
GTAGTGCAGA ATATTACTGT GTGTGAGGGT CAGATGAAAG AGCTGGACTC TTCCATCATC 
AGCGCTGTGG ACCTGGACAT TCCCCAGGAT GCCCTGCTGT TCAGCATCAC TCAAAAGCCA 
CGCCATGGCC TCCTCATCGA TAGGGGGTTT AGCAAAGACT TCTCTGAGAA TAAGCAGCCA 
GCCAACCCTC ACCAGAAACA TGCACCTGTT CACAGCTTTT CCATGGAACT CCTCAAGACT 
GGAATGAGGT TGACGTACAT GCATGATGAC TCAGAGAGCC TTGCTGATGA TTTTACAATC 
CAATTGTCAG ATGGGAAACA TAAGATACTT AAAACCATTT CAGTAGAGGT CATCCCAGTT 
AATGATGAAA AACCAATGCT GAGCAAGAAG GCTGAAATTG CAATGAATAT GGGTGAAACT 
CGTATTATTT CCAGTGCTAT TCTTTCAGCC ATAGATGAAG ACTCACCCAG GGAGAAGATT 
TACTATGTAT TTGAAAGGCT TCCCCAAAAT GGGCAACTTC AGCTTAAGAT AGGGAGGGAC 
TGGGTTCCTC TCTCCCCTGG CATGAAATGC ACTCAGGAGG AAGTGGATCT GAACTTGCTG 
AGATACACAC ACACCGGGGC AATGGATTCC CAGAATCAAG ATAGCTTCAC CTTCTACCTT 
TGGGATGGCA ACAACAGGTC CCCTGCTCTT GACTGTCAAA TCACCATCAA GGACATGGAA 
AAAGGTGATA TTGTCATCCT TACAAAACCA CTCGTGGTGT CTAAAGGTGA TAGAGGTTTC 
TTAACAACCA CCACGCTCCT GGCTGTGGAC GGAACAGACA AGCCTGAGGA ACTGCTCTAT 
GTCATCACCT CCCCTCCGCG ATATGGCCAG ATCGAATATG TTCACTATCC TGGAGTTCCC 
ATTACAAACT TCAGCCAAAT GGATGTAGTG GGGCAGACAG TTTGCTATGT ACACAAGAGC 
AAGGTGACTG TCTCCAGTGA CAGATTCAGA TTCATCATCA GCAATGGACT GCGGACCGAG 
CACGGGGTGT TTGAGATCAC ACTGGAGACT GTGGACAGAG CCCTGCCTGT GGTAACCAGG 
AACAAGGGGT TGAGACTGGC CCAAGGGGCC GTGGGCCTGC TTTCCCCTGA CCTCCTTCAG 
CTGACCGACC CTGATACACC TGCGGAGAAC CTCACCTTCC TCTTGGTTCA GCTCCCCCAG 
CATGGCCAGC TCTACCTGTG GGGGACAGGG CTACTTCAAC ACAATTTCAC CCAGCAGGAT 
GTGGACAGCA AGAATGTGGC CTATCGGCAC TCAGGAGGGG ACTCCCAGAC TGACTGCTTT 
ACTTTCATGG CCACAGATGG GACAAACCAA GGCTTTATTG TGAATGGGAG AGTGTGGGAA 
GAACCTGTTT TATTCACCAT TCAGGTAGAC CAATTGGACA AAACAGCTCC TCGTATCACA 
CTCTTGCATT CCCCTTCTCA AGTGGGGCTC CTGAAAAATG GCTGCTACGG GATTTACATC 
ACTTCCCGCG TGTTGAAGGC ATCAGACCCT GACACTGAGG ACGATCAGAT CATCTTTAAA 
ATTCTACAAG GCCCAAAACA TGGACATCTG GAGAACACAA CAACAGGTGA ATTTATCCAT 
GAGAAATTTA GCCAAAAGGA CTTAAACAGT AAGACTATTC TTTACATCAT AAACCCATCT 
TTGGAAGTAA ATTCAGATAC CGTGGAATTT CAAATCATGG ACCCCACAGG GAACTCGGCC 
ACTCCTCAAA TTTTGGAACT GAAGTGGTCT CATATTGAAT GGTCACAGAC CGAATATGAA 
GTCTGTGAGA ATGTGGGTTT GTTGCCCTTG GAAATTATCA GAAGGGGATA TTCCATGGAC 
TCGGCCTTTG TGGGTATAAA GGTCAACCAA GTGTCAGCTG CAGTTGGAAA AGATTTCACC 
GTGATTCCAT CTAAACTGAT TCAGTTTGAC CCAGGTGCAA GTGGAAATAA AAAAGGCTGG 
TACTTGCTCT CAAGTGTGTT CCATTGTGTG AGAAACACAG ACAAGAATTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016869817.1
CDS	787..6297
Translation	MNSLSWGAANAVLLLLLLAWASPTFISINRGVRVMKGHSAFLSGDDLKFAIPKEKDACKVEVVMNEPITQRVGKLTPQVFDCHFLPNEVKYVHNGCPILDEDTVKLRLYRFTERDTFIETFILWVYLLEPDCNIIHMSNNVLEVPEFNGLSQAIDKNLLRFDYDRMASLECTVSLDTARTRLPAHGQMVLGEPRPEEPRGDQPHSFFPESRMMSFNAFLELRAKLKCPGGSCTPGLKKIGSLKVSCEEFLLMGLRYQHLDPPSPNIDYISIQLDLTDTRSKIVYKSESAWLPVYIRAGIPNQIPKAAFMAVFILEVDQFILTSLTTSVLDCEEDETPKPLLVFNITKAPLQGYVTHLLDHTRPISSFTWKDLSDMQIAYQPPNSSHSERRHDEVELEVYDFFFERSAPMTVHISIRTADTNAPRVSWNTGLSLLEGQSRAITWEQFQVVDNDDIGAVRLVTVGGLQHGWLTLRGGKGFLFTVADLQAGVVRYHHDDSDSTKDFVVFRIFDGHHSIRHKFPINVLPKDDSPPFLITNVVIELEEGQTILIQGSMLRASDVDASDDYIFFNITKPPQAGEIMKKPGPGLIGYPVHGFLQRDLFNGIIYYRHFGGEIFEDSFQFVLWDSHEPPNLSVPQVATIHITPVDDQLPKEAPGVSRHLVVKETEVAYITKKQLHFIDSESYDRELVYTITTPPFFSFSHRHLDAGKLFMVDSIPKVVKNPTALELRSFTQHAVNYMKVAYMPPMQDIGPHCRDVQFTFSVSNQHGGTLHGICFNITILPVDNQVPEAFTNPLKVTEGGQSIISTEHILISDADTKLDNIDLSLRELPLHGRVELNGFPLNSGGTFSWGDLHTLKVRYQHDGTEVLQDDLLLEVTDGTNSAEFVLHVEVFPVNDEPPVLKADLMPVMNCSEGGEVVITSEYIFATDVDSDNLKLMFVIAREPQHGVVRRAGVTVDQFSQRDVISEAVTYKHTGGEIGLMPCFDTITLVVSDGEAGPFVNGCCYNGPNPSVPLHASFPVYDLNITVYPVDNQPPSIAIGPVFVVDEGCSTALTVNHLSATDPDTAADDLEFVLVSPPQFGYLENILPSVGFEKSNIGISIDSFQWKDMNAFHINYVQSRHLRIEPTADQFTVYVTDGKHHSLEIPFSIIINPTNDEAPDFVVQNITVCEGQMKELDSSIISAVDLDIPQDALLFSITQKPRHGLLIDRGFSKDFSENKQPANPHQKHAPVHSFSMELLKTGMRLTYMHDDSESLADDFTIQLSDGKHKILKTISVEVIPVNDEKPMLSKKAEIAMNMGETRIISSAILSAIDEDSPREKIYYVFERLPQNGQLQLKIGRDWVPLSPGMKCTQEEVDLNLLRYTHTGAMDSQNQDSFTFYLWDGNNRSPALDCQITIKDMEKGDIVILTKPLVVSKGDRGFLTTTTLLAVDGTDKPEELLYVITSPPRYGQIEYVHYPGVPITNFSQMDVVGQTVCYVHKSKVTVSSDRFRFIISNGLRTEHGVFEITLETVDRALPVVTRNKGLRLAQGAVGLLSPDLLQLTDPDTPAENLTFLLVQLPQHGQLYLWGTGLLQHNFTQQDVDSKNVAYRHSGGDSQTDCFTFMATDGTNQGFIVNGRVWEEPVLFTIQVDQLDKTAPRITLLHSPSQVGLLKNGCYGIYITSRVLKASDPDTEDDQIIFKILQGPKHGHLENTTTGEFIHEKFSQKDLNSKTILYIINPSLEVNSDTVEFQIMDPTGNSATPQILELKWSHIEWSQTEYEVCENVGLLPLEIIRRGYSMDSAFVGIKVNQVSAAVGKDFTVIPSKLIQFDPGASGNKKGWYLLSSVFHCVRNTDKN

Target ORF information:

RefSeq Version	XM_017014328.2
Organism	Homo sapiens(human)
Definition	Homo sapiens FRAS1 related extracellular matrix 1 (FREM1), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017014328.2

ORF Insert Sequence:

ATGAACTCTC TGAGTTGGGG GGCTGCGAAT GCCGTGCTGC TGCTGCTCCT CCTGGCCTGG 
GCCAGCCCCA CCTTCATCAG CATCAACCGC GGGGTGAGGG TGATGAAGGG CCACTCTGCC 
TTCCTGTCAG GAGATGACCT GAAGTTTGCC ATCCCTAAAG AGAAAGATGC CTGCAAAGTG 
GAAGTTGTGA TGAATGAGCC AATAACCCAG AGGGTTGGGA AACTCACTCC ACAGGTCTTT 
GACTGCCATT TCCTTCCCAA CGAAGTCAAG TATGTTCACA ATGGTTGTCC AATTCTTGAT 
GAAGACACAG TGAAGCTCAG ACTTTACAGA TTTACTGAAA GAGATACCTT CATAGAAACT 
TTTATCCTGT GGGTCTATCT CCTGGAACCA GACTGTAACA TCATCCATAT GAGTAACAAT 
GTGCTGGAGG TGCCTGAATT CAATGGCTTG TCCCAAGCGA TTGATAAAAA TCTGCTCAGA 
TTCGATTATG ATAGGATGGC TAGCCTGGAA TGTACCGTCA GCCTGGACAC TGCGAGAACT 
CGGCTGCCAG CCCATGGCCA GATGGTTCTC GGGGAGCCTC GACCAGAAGA ACCTCGTGGA 
GATCAGCCAC ACAGTTTTTT CCCAGAGTCC CGTATGATGT CTTTTAATGC CTTTCTGGAA 
CTGAGAGCAA AACTGAAGTG TCCAGGTGGG AGCTGTACCC CAGGATTAAA GAAAATAGGA 
AGCCTCAAAG TGAGCTGTGA GGAGTTCCTG CTGATGGGCC TTCGTTATCA GCATCTGGAT 
CCCCCTTCAC CCAACATTGA TTATATCTCC ATCCAACTGG ACCTCACAGA TACCAGGAGC 
AAAATTGTGT ACAAGTCAGA GAGTGCGTGG CTGCCTGTCT ATATCAGAGC TGGAATTCCG 
AATCAGATTC CAAAGGCTGC ATTCATGGCC GTGTTTATTC TGGAAGTGGA TCAGTTCATC 
CTGACCTCCT TGACTACATC AGTTCTGGAC TGTGAAGAAG ATGAGACCCC TAAACCCTTG 
CTGGTGTTCA ACATTACTAA AGCCCCGCTC CAGGGCTATG TGACTCACCT GTTGGATCAC 
ACCAGACCAA TCTCCTCATT CACCTGGAAA GATCTCAGTG ACATGCAGAT CGCCTATCAG 
CCACCAAACA GCAGCCATTC TGAGAGGAGA CATGATGAGG TAGAATTGGA GGTATACGAC 
TTCTTCTTTG AAAGGAGTGC ACCTATGACA GTCCACATCT CCATCAGAAC AGCAGATACA 
AATGCCCCCC GTGTATCCTG GAATACAGGT CTGAGTCTCC TTGAGGGGCA GTCTCGAGCC 
ATCACTTGGG AACAGTTTCA GGTTGTCGAC AATGACGACA TTGGTGCTGT CCGGCTAGTC 
ACCGTTGGTG GCCTGCAGCA TGGATGGCTG ACTTTAAGAG GGGGGAAAGG GTTTCTCTTC 
ACCGTGGCTG ACCTCCAGGC TGGAGTTGTT CGCTATCATC ATGATGACAG CGACTCCACC 
AAAGACTTCG TGGTCTTCCG GATATTTGAT GGCCATCACA GCATCCGTCA CAAATTCCCC 
ATCAACGTCT TGCCCAAAGA TGATAGTCCC CCGTTCCTCA TAACCAATGT TGTGATTGAA 
CTGGAGGAGG GGCAGACCAT CCTGATCCAG GGATCCATGC TGCGAGCTTC AGATGTGGAC 
GCCAGTGATG ACTACATCTT CTTCAATATC ACAAAGCCTC CACAGGCTGG GGAGATCATG 
AAGAAGCCAG GGCCAGGACT GATAGGCTAT CCTGTCCATG GCTTCCTTCA GAGGGATTTG 
TTTAATGGAA TCATTTATTA TCGTCATTTT GGTGGAGAAA TCTTTGAAGA TTCTTTTCAA 
TTTGTCCTGT GGGACAGCCA TGAACCTCCA AATCTTTCAG TGCCACAGGT GGCAACAATC 
CATATAACTC CAGTGGATGA CCAGCTTCCA AAAGAGGCTC CTGGAGTTTC TCGGCATTTG 
GTTGTCAAGG AAACTGAGGT GGCCTATATA ACTAAGAAAC AGCTACATTT TATAGATTCA 
GAATCATATG ACAGGGAGCT GGTCTACACA ATAACTACTC CTCCATTTTT CTCCTTCAGC 
CACAGACACT TGGATGCTGG GAAATTATTT ATGGTGGACA GCATACCAAA AGTAGTTAAG 
AATCCTACGG CCCTGGAGCT GAGGTCATTC ACTCAGCATG CTGTGAACTA TATGAAAGTG 
GCCTACATGC CCCCCATGCA AGACATTGGT CCCCATTGCA GAGATGTCCA GTTCACATTT 
TCTGTCAGTA ACCAACATGG CGGTACTTTG CATGGGATCT GCTTTAACAT TACAATCCTC 
CCAGTGGACA ATCAAGTTCC TGAGGCGTTC ACCAACCCTC TGAAAGTGAC TGAGGGAGGT 
CAAAGCATCA TCAGCACAGA GCACATTCTA ATTTCTGATG CAGATACCAA GCTGGACAAT 
ATTGACCTCT CCCTGCGGGA ATTGCCTCTG CACGGAAGGG TGGAGCTGAA TGGATTTCCT 
CTAAATTCAG GGGGCACATT TTCTTGGGGC GATCTCCATA CCTTAAAAGT TAGGTATCAA 
CATGATGGAA CTGAAGTTCT TCAGGATGAC CTACTCTTGG AGGTCACCGA TGGCACAAAT 
TCAGCAGAAT TTGTACTACA TGTTGAGGTA TTCCCTGTCA ACGATGAGCC ACCAGTCTTA 
AAGGCTGACC TCATGCCTGT CATGAATTGC TCAGAGGGAG GAGAGGTGGT CATCACCTCT 
GAATACATTT TTGCTACTGA TGTGGACAGC GATAACTTGA AGTTGATGTT TGTGATTGCT 
CGCGAACCTC AGCATGGGGT GGTGAGGAGA GCTGGAGTCA CAGTGGATCA GTTCTCTCAG 
AGAGATGTTA TCTCAGAGGC CGTGACATAC AAACACACAG GTGGCGAGAT TGGCCTGATG 
CCTTGTTTTG ACACCATTAC ATTGGTGGTT TCGGATGGAG AGGCTGGCCC TTTTGTGAAT 
GGCTGTTGCT ACAATGGACC CAATCCATCT GTTCCACTTC ATGCGTCCTT TCCAGTGTAT 
GATCTCAACA TCACGGTATA CCCAGTAGAC AACCAGCCAC CTTCCATTGC AATAGGTCCC 
GTGTTTGTTG TAGATGAGGG CTGCTCAACA GCCCTTACTG TTAACCATTT GTCCGCCACT 
GACCCTGACA CTGCAGCAGA TGACTTGGAA TTTGTTTTGG TTTCTCCTCC TCAGTTTGGC 
TACCTCGAAA ATATACTCCC TTCTGTGGGT TTTGAAAAAA GCAATATTGG CATAAGTATA 
GATTCATTTC AGTGGAAAGA CATGAACGCT TTTCACATTA ACTATGTGCA GTCCAGGCAT 
CTGAGGATAG AACCAACTGC CGACCAGTTC ACGGTGTACG TCACAGATGG GAAGCATCAC 
TCCTTGGAGA TACCATTTTC TATTATAATC AACCCCACAA ATGATGAAGC TCCTGACTTT 
GTAGTGCAGA ATATTACTGT GTGTGAGGGT CAGATGAAAG AGCTGGACTC TTCCATCATC 
AGCGCTGTGG ACCTGGACAT TCCCCAGGAT GCCCTGCTGT TCAGCATCAC TCAAAAGCCA 
CGCCATGGCC TCCTCATCGA TAGGGGGTTT AGCAAAGACT TCTCTGAGAA TAAGCAGCCA 
GCCAACCCTC ACCAGAAACA TGCACCTGTT CACAGCTTTT CCATGGAACT CCTCAAGACT 
GGAATGAGGT TGACGTACAT GCATGATGAC TCAGAGAGCC TTGCTGATGA TTTTACAATC 
CAATTGTCAG ATGGGAAACA TAAGATACTT AAAACCATTT CAGTAGAGGT CATCCCAGTT 
AATGATGAAA AACCAATGCT GAGCAAGAAG GCTGAAATTG CAATGAATAT GGGTGAAACT 
CGTATTATTT CCAGTGCTAT TCTTTCAGCC ATAGATGAAG ACTCACCCAG GGAGAAGATT 
TACTATGTAT TTGAAAGGCT TCCCCAAAAT GGGCAACTTC AGCTTAAGAT AGGGAGGGAC 
TGGGTTCCTC TCTCCCCTGG CATGAAATGC ACTCAGGAGG AAGTGGATCT GAACTTGCTG 
AGATACACAC ACACCGGGGC AATGGATTCC CAGAATCAAG ATAGCTTCAC CTTCTACCTT 
TGGGATGGCA ACAACAGGTC CCCTGCTCTT GACTGTCAAA TCACCATCAA GGACATGGAA 
AAAGGTGATA TTGTCATCCT TACAAAACCA CTCGTGGTGT CTAAAGGTGA TAGAGGTTTC 
TTAACAACCA CCACGCTCCT GGCTGTGGAC GGAACAGACA AGCCTGAGGA ACTGCTCTAT 
GTCATCACCT CCCCTCCGCG ATATGGCCAG ATCGAATATG TTCACTATCC TGGAGTTCCC 
ATTACAAACT TCAGCCAAAT GGATGTAGTG GGGCAGACAG TTTGCTATGT ACACAAGAGC 
AAGGTGACTG TCTCCAGTGA CAGATTCAGA TTCATCATCA GCAATGGACT GCGGACCGAG 
CACGGGGTGT TTGAGATCAC ACTGGAGACT GTGGACAGAG CCCTGCCTGT GGTAACCAGG 
AACAAGGGGT TGAGACTGGC CCAAGGGGCC GTGGGCCTGC TTTCCCCTGA CCTCCTTCAG 
CTGACCGACC CTGATACACC TGCGGAGAAC CTCACCTTCC TCTTGGTTCA GCTCCCCCAG 
CATGGCCAGC TCTACCTGTG GGGGACAGGG CTACTTCAAC ACAATTTCAC CCAGCAGGAT 
GTGGACAGCA AGAATGTGGC CTATCGGCAC TCAGGAGGGG ACTCCCAGAC TGACTGCTTT 
ACTTTCATGG CCACAGATGG GACAAACCAA GGCTTTATTG TGAATGGGAG AGTGTGGGAA 
GAACCTGTTT TATTCACCAT TCAGGTAGAC CAATTGGACA AAACAGCTCC TCGTATCACA 
CTCTTGCATT CCCCTTCTCA AGTGGGGCTC CTGAAAAATG GCTGCTACGG GATTTACATC 
ACTTCCCGCG TGTTGAAGGC ATCAGACCCT GACACTGAGG ACGATCAGAT CATCTTTAAA 
ATTCTACAAG GCCCAAAACA TGGACATCTG GAGAACACAA CAACAGGTGA ATTTATCCAT 
GAGAAATTTA GCCAAAAGGA CTTAAACAGT AAGACTATTC TTTACATCAT AAACCCATCT 
TTGGAAGTAA ATTCAGATAC CGTGGAATTT CAAATCATGG ACCCCACAGG GAACTCGGCC 
ACTCCTCAAA TTTTGGAACT GAAGTGGTCT CATATTGAAT GGTCACAGAC CGAATATGAA 
GTCTGTGAGA ATGTGGGTTT GTTGCCCTTG GAAATTATCA GAAGGGGATA TTCCATGGAC 
TCGGCCTTTG TGGGTATAAA GGTCAACCAA GTGTCAGCTG CAGTTGGAAA AGATTTCACC 
GTGATTCCAT CTAAACTGAT TCAGTTTGAC CCAGGTGCAA GTGGAAATAA AAAAGGCTGG 
TACTTGCTCT CAAGTGTGTT CCATTGTGTG AGAAACACAG ACAAGAATTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FREM1 ( XM_017014329.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016869818.1 Homo sapiens FRAS1 related extracellular matrix 1 (FREM1), transcript variant X15, mRNA.

Price & Availability↑

CloneID	OHu98465
Clone ID Related Accession (Same CDS sequence)	XM_017014329.1 , XM_017014329.2
Accession Version	XM_017014329.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5451bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	FRAS1-related extracellular matrix protein 1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACTCTC TGAGTTGGGG GGCTGCGAAT GCCGTGCTGC TGCTGCTCCT CCTGGCCTGG 
GCCAGCCCCA CCTTCATCAG CATCAACCGC GGGGTGAGGG TGATGAAGGG CCACTCTGCC 
TTCCTGTCAG GAGATGACCT GAAGTTTGCC ATCCCTAAAG AGAAAGATGC CTGCAAAGTG 
GAAGTTGTGA TGAATGAGCC AATAACCCAG AGGGTTGGGA AACTCACTCC ACAGGTCTTT 
GACTGCCATT TCCTTCCCAA CGAAGTCAAG TATGTTCACA ATGGTTGTCC AATTCTTGAT 
GAAGACACAG TGAAGCTCAG ACTTTACAGA TTTACTGAAA GAGATACCTT CATAGAAACT 
TTTATCCTGT GGGTCTATCT CCTGGAACCA GACTGTAACA TCATCCATAT GAGTAACAAT 
GTGCTGGAGG TGCCTGAATT CAATGGCTTG TCCCAAGCGA TTGATAAAAA TCTGCTCAGA 
TTCGATTATG ATAGGATGGC TAGCCTGGAA TGTACCGTCA GCCTGGACAC TGCGAGAACT 
CGGCTGCCAG CCCATGGCCA GATGGTTCTC GGGGAGCCTC GACCAGAAGA ACCTCGTGGA 
GATCAGCCAC ACAGTTTTTT CCCAGAGTCC CGTATGATGT CTTTTAATGC CTTTCTGGAA 
CTGAGAGCAA AACTGAAGTG TCCAGGTGGG AGCTGTACCC CAGGATTAAA GAAAATAGGA 
AGCCTCAAAG TGAGCTGTGA GGAGTTCCTG CTGATGGGCC TTCGTTATCA GCATCTGGAT 
CCCCCTTCAC CCAACATTGA TTATATCTCC ATCCAACTGG ACCTCACAGA TACCAGGAGC 
AAAATTGTGT ACAAGTCAGA GAGTGCGTGG CTGCCTGTCT ATATCAGAGC TGGAATTCCG 
AATCAGATTC CAAAGGCTGC ATTCATGGCC GTGTTTATTC TGGAAGTGGA TCAGTTCATC 
CTGACCTCCT TGACTACATC AGTTCTGGAC TGTGAAGAAG ATGAGACCCC TAAACCCTTG 
CTGGTGTTCA ACATTACTAA AGCCCCGCTC CAGGGCTATG TGACTCACCT GTTGGATCAC 
ACCAGACCAA TCTCCTCATT CACCTGGAAA GATCTCAGTG ACATGCAGAT CGCCTATCAG 
CCACCAAACA GCAGCCATTC TGAGAGGAGA CATGATGAGG TAGAATTGGA GGTATACGAC 
TTCTTCTTTG AAAGGAGTGC ACCTATGACA GTCCACATCT CCATCAGAAC AGCAGATACA 
AATGCCCCCC GTGTATCCTG GAATACAGGT CTGAGTCTCC TTGAGGGGCA GTCTCGAGCC 
ATCACTTGGG AACAGTTTCA GGTTGTCGAC AATGACGACA TTGGTGCTGT CCGGCTAGTC 
ACCGTTGGTG GCCTGCAGCA TGGATGGCTG ACTTTAAGAG GGGGGAAAGG GTTTCTCTTC 
ACCGTGGCTG ACCTCCAGGC TGGAGTTGTT CGCTATCATC ATGATGACAG CGACTCCACC 
AAAGACTTCG TGGTCTTCCG GATATTTGAT GGCCATCACA GCATCCGTCA CAAATTCCCC 
ATCAACGTCT TGCCCAAAGA TGATAGTCCC CCGTTCCTCA TAACCAATGT TGTGATTGAA 
CTGGAGGAGG GGCAGACCAT CCTGATCCAG GGATCCATGC TGCGAGCTTC AGATGTGGAC 
GCCAGTGATG ACTACATCTT CTTCAATATC ACAAAGCCTC CACAGGCTGG GGAGATCATG 
AAGAAGCCAG GGCCAGGACT GATAGGCTAT CCTGTCCATG GCTTCCTTCA GAGGGATTTG 
TTTAATGGAA TCATTTATTA TCGTCATTTT GGTGGAGAAA TCTTTGAAGA TTCTTTTCAA 
TTTGTCCTGT GGGACAGCCA TGAACCTCCA AATCTTTCAG TGCCACAGGT GGCAACAATC 
CATATAACTC CAGTGGATGA CCAGCTTCCA AAAGAGGCTC CTGGAGTTTC TCGGCATTTG 
GTTGTCAAGG AAACTGAGGT GGCCTATATA ACTAAGAAAC AGCTACATTT TATAGATTCA 
GAATCATATG ACAGGGAGCT GGTCTACACA ATAACTACTC CTCCATTTTT CTCCTTCAGC 
CACAGACACT TGGATGCTGG GAAATTATTT ATGGTGGACA GCATACCAAA AGTAGTTAAG 
AATCCTACGG CCCTGGAGCT GAGGTCATTC ACTCAGCATG CTGTGAACTA TATGAAAGTG 
GCCTACATGC CCCCCATGCA AGACATTGGT CCCCATTGCA GAGATGTCCA GTTCACATTT 
TCTGTCAGTA ACCAACATGG CGGTACTTTG CATGGGATCT GCTTTAACAT TACAATCCTC 
CCAGTGGACA ATCAAGTTCC TGAGGCGTTC ACCAACCCTC TGAAAGTGAC TGAGGGAGGT 
CAAAGCATCA TCAGCACAGA GCACATTCTA ATTTCTGATG CAGATACCAA GCTGGACAAT 
ATTGACCTCT CCCTGCGGGA ATTGCCTCTG CACGGAAGGG TGGAGCTGAA TGGATTTCCT 
CTAAATTCAG GGGGCACATT TTCTTGGGGC GATCTCCATA CCTTAAAAGT TAGGTATCAA 
CATGATGGAA CTGAAGTTCT TCAGGATGAC CTACTCTTGG AGGTCACCGA TGGCACAAAT 
TCAGCAGAAT TTGTACTACA TGTTGAGGTA TTCCCTGTCA ACGATGAGCC ACCAGTCTTA 
AAGGCTGACC TCATGCCTGT CATGAATTGC TCAGAGGGAG GAGAGGTGGT CATCACCTCT 
GAATACATTT TTGCTACTGA TGTGGACAGC GATAACTTGA AGTTGATGTT TGTGATTGCT 
CGCGAACCTC AGCATGGGGT GGTGAGGAGA GCTGGAGTCA CAGTGGATCA GTTCTCTCAG 
AGAGATGTTA TCTCAGAGGC CGTGACATAC AAACACACAG GTGGCGAGAT TGGCCTGATG 
CCTTGTTTTG ACACCATTAC ATTGGTGGTT TCGGATGGAG AGGCTGGCCC TTTTGTGAAT 
GGCTGTTGCT ACAATGGACC CAATCCATCT GTTCCACTTC ATGCGTCCTT TCCAGTGTAT 
GATCTCAACA TCACGGTATA CCCAGTAGAC AACCAGCCAC CTTCCATTGC AATAGGTCCC 
GTGTTTGTTG TAGATGAGGG CTGCTCAACA GCCCTTACTG TTAACCATTT GTCCGCCACT 
GACCCTGACA CTGCAGCAGA TGACTTGGAA TTTGTTTTGG TTTCTCCTCC TCAGTTTGGC 
TACCTCGAAA ATATACTCCC TTCTGTGGGT TTTGAAAAAA GCAATATTGG CATAAGTATA 
GATTCATTTC AGTGGAAAGA CATGAACGCT TTTCACATTA ACTATGTGCA GTCCAGGCAT 
CTGAGGATAG AACCAACTGC CGACCAGTTC ACGGTGTACG TCACAGATGG GAAGCATCAC 
TCCTTGGAGA TACCATTTTC TATTATAATC AACCCCACAA ATGATGAAGC TCCTGACTTT 
GTAGTGCAGA ATATTACTGT GTGTGAGGGT CAGATGAAAG AGCTGGACTC TTCCATCATC 
AGCGCTGTGG ACCTGGACAT TCCCCAGGAT GCCCTGCTGT TCAGCATCAC TCAAAAGCCA 
CGCCATGGCC TCCTCATCGA TAGGGGGTTT AGCAAAGACT TCTCTGAGAA TAAGCAGCCA 
GCCAACCCTC ACCAGAAACA TGCACCTGTT CACAGCTTTT CCATGGAACT CCTCAAGACT 
GGAATGAGGT TGACGTACAT GCATGATGAC TCAGAGAGCC TTGCTGATGA TTTTACAATC 
CAATTGTCAG ATGGGAAACA TAAGATACTT AAAACCATTT CAGTAGAGGT CATCCCAGTT 
AATGATGAAA AACCAATGCT GAGCAAGAAG GCTGAAATTG CAATGAATAT GGGTGAAACT 
CGTATTATTT CCAGTGCTAT TCTTTCAGCC ATAGATGAAG ACTCACCCAG GGAGAAGATT 
TACTATGTAT TTGAAAGGCT TCCCCAAAAT GGGCAACTTC AGCTTAAGAT AGGGAGGGAC 
TGGGTTCCTC TCTCCCCTGG CATGAAATGC ACTCAGGAGG AAGTGGATCT GAACTTGCTG 
AGATACACAC ACACCGGGGC AATGGATTCC CAGAATCAAG ATAGCTTCAC CTTCTACCTT 
TGGGATGGCA ACAACAGGTC CCCTGCTCTT GACTGTCAAA TCACCATCAA GGACATGGAA 
AAAGGTGATA TTGTCATCCT TACAAAACCA CTCGTGGTGT CTAAAGGTGA TAGAGGTTTC 
TTAACAACCA CCACGCTCCT GGCTGTGGAC GGAACAGACA AGCCTGAGGA ACTGCTCTAT 
GTCATCACCT CCCCTCCGCG ATATGGCCAG ATCGAATATG TTCACTATCC TGGAGTTCCC 
ATTACAAACT TCAGCCAAAT GGATGTAGTG GGGCAGACAG TTTGCTATGT ACACAAGAGC 
AAGGTGACTG TCTCCAGTGA CAGATTCAGA TTCATCATCA GCAATGGACT GCGGACCGAG 
CACGGGGTGT TTGAGATCAC ACTGGAGACT GTGGACAGAG CCCTGCCTGT GGTAACCAGG 
AACAAGGGGT TGAGACTGGC CCAAGGGGCC GTGGGCCTGC TTTCCCCTGA CCTCCTTCAG 
CTGACCGACC CTGATACACC TGCGGAGAAC CTCACCTTCC TCTTGGTTCA GCTCCCCCAG 
CATGGCCAGC TCTACCTGTG GGGGACAGGG CTACTTCAAC ACAATTTCAC CCAGCAGGAT 
GTGGACAGCA AGAATGTGGC CTATCGGCAC TCAGGAGGGG ACTCCCAGAC TGACTGCTTT 
ACTTTCATGG CCACAGATGG GACAAACCAA GGCTTTATTG TGAATGGGAG AGTGTGGGAA 
GAACCTGTTT TATTCACCAT TCAGGTAGAC CAATTGGACA AAACAGCTCC TCGTATCACA 
CTCTTGCATT CCCCTTCTCA AGTGGGGCTC CTGAAAAATG GCTGCTACGG GATTTACATC 
ACTTCCCGCG TGTTGAAGGC ATCAGACCCT GACACTGAGG ACGATCAGAT CATCTTTAAA 
ATTCTACAAG GCCCAAAACA TGGACATCTG GAGAACACAA CAACAGGTGA ATTTATCCAT 
GAGAAATTTA GCCAAAAGGA CTTAAACAGT AAGACTATTC TTTACATCAT AAACCCATCT 
TTGGAAGTAA ATTCAGATAC CGTGGAATTT CAAATCATGG ACCCCACAGG GAACTCGGCC 
ACTCCTCAAA TTTTGGAACT GAAGTGGTCT CATATTGAAT GGTCACAGAC CGAATATGAA 
GTCTGTGAGA ATGTGGGTTT GTTGCCCTTG GAAATTATCA GAAGGGGATA TTCCATGGAC 
TCGGCCTTTG TGGGTATAAA GGTCAACCAA GTGTCAGCTG CAGTTGGAAA AGATTTCACC 
GTGATTCCAT CTAAACTGAT TCAGTTTGAC CCAGCTTCCA TGATTGGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016869818.1
CDS	1065..6515
Translation	MNSLSWGAANAVLLLLLLAWASPTFISINRGVRVMKGHSAFLSGDDLKFAIPKEKDACKVEVVMNEPITQRVGKLTPQVFDCHFLPNEVKYVHNGCPILDEDTVKLRLYRFTERDTFIETFILWVYLLEPDCNIIHMSNNVLEVPEFNGLSQAIDKNLLRFDYDRMASLECTVSLDTARTRLPAHGQMVLGEPRPEEPRGDQPHSFFPESRMMSFNAFLELRAKLKCPGGSCTPGLKKIGSLKVSCEEFLLMGLRYQHLDPPSPNIDYISIQLDLTDTRSKIVYKSESAWLPVYIRAGIPNQIPKAAFMAVFILEVDQFILTSLTTSVLDCEEDETPKPLLVFNITKAPLQGYVTHLLDHTRPISSFTWKDLSDMQIAYQPPNSSHSERRHDEVELEVYDFFFERSAPMTVHISIRTADTNAPRVSWNTGLSLLEGQSRAITWEQFQVVDNDDIGAVRLVTVGGLQHGWLTLRGGKGFLFTVADLQAGVVRYHHDDSDSTKDFVVFRIFDGHHSIRHKFPINVLPKDDSPPFLITNVVIELEEGQTILIQGSMLRASDVDASDDYIFFNITKPPQAGEIMKKPGPGLIGYPVHGFLQRDLFNGIIYYRHFGGEIFEDSFQFVLWDSHEPPNLSVPQVATIHITPVDDQLPKEAPGVSRHLVVKETEVAYITKKQLHFIDSESYDRELVYTITTPPFFSFSHRHLDAGKLFMVDSIPKVVKNPTALELRSFTQHAVNYMKVAYMPPMQDIGPHCRDVQFTFSVSNQHGGTLHGICFNITILPVDNQVPEAFTNPLKVTEGGQSIISTEHILISDADTKLDNIDLSLRELPLHGRVELNGFPLNSGGTFSWGDLHTLKVRYQHDGTEVLQDDLLLEVTDGTNSAEFVLHVEVFPVNDEPPVLKADLMPVMNCSEGGEVVITSEYIFATDVDSDNLKLMFVIAREPQHGVVRRAGVTVDQFSQRDVISEAVTYKHTGGEIGLMPCFDTITLVVSDGEAGPFVNGCCYNGPNPSVPLHASFPVYDLNITVYPVDNQPPSIAIGPVFVVDEGCSTALTVNHLSATDPDTAADDLEFVLVSPPQFGYLENILPSVGFEKSNIGISIDSFQWKDMNAFHINYVQSRHLRIEPTADQFTVYVTDGKHHSLEIPFSIIINPTNDEAPDFVVQNITVCEGQMKELDSSIISAVDLDIPQDALLFSITQKPRHGLLIDRGFSKDFSENKQPANPHQKHAPVHSFSMELLKTGMRLTYMHDDSESLADDFTIQLSDGKHKILKTISVEVIPVNDEKPMLSKKAEIAMNMGETRIISSAILSAIDEDSPREKIYYVFERLPQNGQLQLKIGRDWVPLSPGMKCTQEEVDLNLLRYTHTGAMDSQNQDSFTFYLWDGNNRSPALDCQITIKDMEKGDIVILTKPLVVSKGDRGFLTTTTLLAVDGTDKPEELLYVITSPPRYGQIEYVHYPGVPITNFSQMDVVGQTVCYVHKSKVTVSSDRFRFIISNGLRTEHGVFEITLETVDRALPVVTRNKGLRLAQGAVGLLSPDLLQLTDPDTPAENLTFLLVQLPQHGQLYLWGTGLLQHNFTQQDVDSKNVAYRHSGGDSQTDCFTFMATDGTNQGFIVNGRVWEEPVLFTIQVDQLDKTAPRITLLHSPSQVGLLKNGCYGIYITSRVLKASDPDTEDDQIIFKILQGPKHGHLENTTTGEFIHEKFSQKDLNSKTILYIINPSLEVNSDTVEFQIMDPTGNSATPQILELKWSHIEWSQTEYEVCENVGLLPLEIIRRGYSMDSAFVGIKVNQVSAAVGKDFTVIPSKLIQFDPASMIG

Target ORF information:

RefSeq Version	XM_017014329.1
Organism	Homo sapiens(human)
Definition	Homo sapiens FRAS1 related extracellular matrix 1 (FREM1), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017014329.1

ORF Insert Sequence:

ATGAACTCTC TGAGTTGGGG GGCTGCGAAT GCCGTGCTGC TGCTGCTCCT CCTGGCCTGG 
GCCAGCCCCA CCTTCATCAG CATCAACCGC GGGGTGAGGG TGATGAAGGG CCACTCTGCC 
TTCCTGTCAG GAGATGACCT GAAGTTTGCC ATCCCTAAAG AGAAAGATGC CTGCAAAGTG 
GAAGTTGTGA TGAATGAGCC AATAACCCAG AGGGTTGGGA AACTCACTCC ACAGGTCTTT 
GACTGCCATT TCCTTCCCAA CGAAGTCAAG TATGTTCACA ATGGTTGTCC AATTCTTGAT 
GAAGACACAG TGAAGCTCAG ACTTTACAGA TTTACTGAAA GAGATACCTT CATAGAAACT 
TTTATCCTGT GGGTCTATCT CCTGGAACCA GACTGTAACA TCATCCATAT GAGTAACAAT 
GTGCTGGAGG TGCCTGAATT CAATGGCTTG TCCCAAGCGA TTGATAAAAA TCTGCTCAGA 
TTCGATTATG ATAGGATGGC TAGCCTGGAA TGTACCGTCA GCCTGGACAC TGCGAGAACT 
CGGCTGCCAG CCCATGGCCA GATGGTTCTC GGGGAGCCTC GACCAGAAGA ACCTCGTGGA 
GATCAGCCAC ACAGTTTTTT CCCAGAGTCC CGTATGATGT CTTTTAATGC CTTTCTGGAA 
CTGAGAGCAA AACTGAAGTG TCCAGGTGGG AGCTGTACCC CAGGATTAAA GAAAATAGGA 
AGCCTCAAAG TGAGCTGTGA GGAGTTCCTG CTGATGGGCC TTCGTTATCA GCATCTGGAT 
CCCCCTTCAC CCAACATTGA TTATATCTCC ATCCAACTGG ACCTCACAGA TACCAGGAGC 
AAAATTGTGT ACAAGTCAGA GAGTGCGTGG CTGCCTGTCT ATATCAGAGC TGGAATTCCG 
AATCAGATTC CAAAGGCTGC ATTCATGGCC GTGTTTATTC TGGAAGTGGA TCAGTTCATC 
CTGACCTCCT TGACTACATC AGTTCTGGAC TGTGAAGAAG ATGAGACCCC TAAACCCTTG 
CTGGTGTTCA ACATTACTAA AGCCCCGCTC CAGGGCTATG TGACTCACCT GTTGGATCAC 
ACCAGACCAA TCTCCTCATT CACCTGGAAA GATCTCAGTG ACATGCAGAT CGCCTATCAG 
CCACCAAACA GCAGCCATTC TGAGAGGAGA CATGATGAGG TAGAATTGGA GGTATACGAC 
TTCTTCTTTG AAAGGAGTGC ACCTATGACA GTCCACATCT CCATCAGAAC AGCAGATACA 
AATGCCCCCC GTGTATCCTG GAATACAGGT CTGAGTCTCC TTGAGGGGCA GTCTCGAGCC 
ATCACTTGGG AACAGTTTCA GGTTGTCGAC AATGACGACA TTGGTGCTGT CCGGCTAGTC 
ACCGTTGGTG GCCTGCAGCA TGGATGGCTG ACTTTAAGAG GGGGGAAAGG GTTTCTCTTC 
ACCGTGGCTG ACCTCCAGGC TGGAGTTGTT CGCTATCATC ATGATGACAG CGACTCCACC 
AAAGACTTCG TGGTCTTCCG GATATTTGAT GGCCATCACA GCATCCGTCA CAAATTCCCC 
ATCAACGTCT TGCCCAAAGA TGATAGTCCC CCGTTCCTCA TAACCAATGT TGTGATTGAA 
CTGGAGGAGG GGCAGACCAT CCTGATCCAG GGATCCATGC TGCGAGCTTC AGATGTGGAC 
GCCAGTGATG ACTACATCTT CTTCAATATC ACAAAGCCTC CACAGGCTGG GGAGATCATG 
AAGAAGCCAG GGCCAGGACT GATAGGCTAT CCTGTCCATG GCTTCCTTCA GAGGGATTTG 
TTTAATGGAA TCATTTATTA TCGTCATTTT GGTGGAGAAA TCTTTGAAGA TTCTTTTCAA 
TTTGTCCTGT GGGACAGCCA TGAACCTCCA AATCTTTCAG TGCCACAGGT GGCAACAATC 
CATATAACTC CAGTGGATGA CCAGCTTCCA AAAGAGGCTC CTGGAGTTTC TCGGCATTTG 
GTTGTCAAGG AAACTGAGGT GGCCTATATA ACTAAGAAAC AGCTACATTT TATAGATTCA 
GAATCATATG ACAGGGAGCT GGTCTACACA ATAACTACTC CTCCATTTTT CTCCTTCAGC 
CACAGACACT TGGATGCTGG GAAATTATTT ATGGTGGACA GCATACCAAA AGTAGTTAAG 
AATCCTACGG CCCTGGAGCT GAGGTCATTC ACTCAGCATG CTGTGAACTA TATGAAAGTG 
GCCTACATGC CCCCCATGCA AGACATTGGT CCCCATTGCA GAGATGTCCA GTTCACATTT 
TCTGTCAGTA ACCAACATGG CGGTACTTTG CATGGGATCT GCTTTAACAT TACAATCCTC 
CCAGTGGACA ATCAAGTTCC TGAGGCGTTC ACCAACCCTC TGAAAGTGAC TGAGGGAGGT 
CAAAGCATCA TCAGCACAGA GCACATTCTA ATTTCTGATG CAGATACCAA GCTGGACAAT 
ATTGACCTCT CCCTGCGGGA ATTGCCTCTG CACGGAAGGG TGGAGCTGAA TGGATTTCCT 
CTAAATTCAG GGGGCACATT TTCTTGGGGC GATCTCCATA CCTTAAAAGT TAGGTATCAA 
CATGATGGAA CTGAAGTTCT TCAGGATGAC CTACTCTTGG AGGTCACCGA TGGCACAAAT 
TCAGCAGAAT TTGTACTACA TGTTGAGGTA TTCCCTGTCA ACGATGAGCC ACCAGTCTTA 
AAGGCTGACC TCATGCCTGT CATGAATTGC TCAGAGGGAG GAGAGGTGGT CATCACCTCT 
GAATACATTT TTGCTACTGA TGTGGACAGC GATAACTTGA AGTTGATGTT TGTGATTGCT 
CGCGAACCTC AGCATGGGGT GGTGAGGAGA GCTGGAGTCA CAGTGGATCA GTTCTCTCAG 
AGAGATGTTA TCTCAGAGGC CGTGACATAC AAACACACAG GTGGCGAGAT TGGCCTGATG 
CCTTGTTTTG ACACCATTAC ATTGGTGGTT TCGGATGGAG AGGCTGGCCC TTTTGTGAAT 
GGCTGTTGCT ACAATGGACC CAATCCATCT GTTCCACTTC ATGCGTCCTT TCCAGTGTAT 
GATCTCAACA TCACGGTATA CCCAGTAGAC AACCAGCCAC CTTCCATTGC AATAGGTCCC 
GTGTTTGTTG TAGATGAGGG CTGCTCAACA GCCCTTACTG TTAACCATTT GTCCGCCACT 
GACCCTGACA CTGCAGCAGA TGACTTGGAA TTTGTTTTGG TTTCTCCTCC TCAGTTTGGC 
TACCTCGAAA ATATACTCCC TTCTGTGGGT TTTGAAAAAA GCAATATTGG CATAAGTATA 
GATTCATTTC AGTGGAAAGA CATGAACGCT TTTCACATTA ACTATGTGCA GTCCAGGCAT 
CTGAGGATAG AACCAACTGC CGACCAGTTC ACGGTGTACG TCACAGATGG GAAGCATCAC 
TCCTTGGAGA TACCATTTTC TATTATAATC AACCCCACAA ATGATGAAGC TCCTGACTTT 
GTAGTGCAGA ATATTACTGT GTGTGAGGGT CAGATGAAAG AGCTGGACTC TTCCATCATC 
AGCGCTGTGG ACCTGGACAT TCCCCAGGAT GCCCTGCTGT TCAGCATCAC TCAAAAGCCA 
CGCCATGGCC TCCTCATCGA TAGGGGGTTT AGCAAAGACT TCTCTGAGAA TAAGCAGCCA 
GCCAACCCTC ACCAGAAACA TGCACCTGTT CACAGCTTTT CCATGGAACT CCTCAAGACT 
GGAATGAGGT TGACGTACAT GCATGATGAC TCAGAGAGCC TTGCTGATGA TTTTACAATC 
CAATTGTCAG ATGGGAAACA TAAGATACTT AAAACCATTT CAGTAGAGGT CATCCCAGTT 
AATGATGAAA AACCAATGCT GAGCAAGAAG GCTGAAATTG CAATGAATAT GGGTGAAACT 
CGTATTATTT CCAGTGCTAT TCTTTCAGCC ATAGATGAAG ACTCACCCAG GGAGAAGATT 
TACTATGTAT TTGAAAGGCT TCCCCAAAAT GGGCAACTTC AGCTTAAGAT AGGGAGGGAC 
TGGGTTCCTC TCTCCCCTGG CATGAAATGC ACTCAGGAGG AAGTGGATCT GAACTTGCTG 
AGATACACAC ACACCGGGGC AATGGATTCC CAGAATCAAG ATAGCTTCAC CTTCTACCTT 
TGGGATGGCA ACAACAGGTC CCCTGCTCTT GACTGTCAAA TCACCATCAA GGACATGGAA 
AAAGGTGATA TTGTCATCCT TACAAAACCA CTCGTGGTGT CTAAAGGTGA TAGAGGTTTC 
TTAACAACCA CCACGCTCCT GGCTGTGGAC GGAACAGACA AGCCTGAGGA ACTGCTCTAT 
GTCATCACCT CCCCTCCGCG ATATGGCCAG ATCGAATATG TTCACTATCC TGGAGTTCCC 
ATTACAAACT TCAGCCAAAT GGATGTAGTG GGGCAGACAG TTTGCTATGT ACACAAGAGC 
AAGGTGACTG TCTCCAGTGA CAGATTCAGA TTCATCATCA GCAATGGACT GCGGACCGAG 
CACGGGGTGT TTGAGATCAC ACTGGAGACT GTGGACAGAG CCCTGCCTGT GGTAACCAGG 
AACAAGGGGT TGAGACTGGC CCAAGGGGCC GTGGGCCTGC TTTCCCCTGA CCTCCTTCAG 
CTGACCGACC CTGATACACC TGCGGAGAAC CTCACCTTCC TCTTGGTTCA GCTCCCCCAG 
CATGGCCAGC TCTACCTGTG GGGGACAGGG CTACTTCAAC ACAATTTCAC CCAGCAGGAT 
GTGGACAGCA AGAATGTGGC CTATCGGCAC TCAGGAGGGG ACTCCCAGAC TGACTGCTTT 
ACTTTCATGG CCACAGATGG GACAAACCAA GGCTTTATTG TGAATGGGAG AGTGTGGGAA 
GAACCTGTTT TATTCACCAT TCAGGTAGAC CAATTGGACA AAACAGCTCC TCGTATCACA 
CTCTTGCATT CCCCTTCTCA AGTGGGGCTC CTGAAAAATG GCTGCTACGG GATTTACATC 
ACTTCCCGCG TGTTGAAGGC ATCAGACCCT GACACTGAGG ACGATCAGAT CATCTTTAAA 
ATTCTACAAG GCCCAAAACA TGGACATCTG GAGAACACAA CAACAGGTGA ATTTATCCAT 
GAGAAATTTA GCCAAAAGGA CTTAAACAGT AAGACTATTC TTTACATCAT AAACCCATCT 
TTGGAAGTAA ATTCAGATAC CGTGGAATTT CAAATCATGG ACCCCACAGG GAACTCGGCC 
ACTCCTCAAA TTTTGGAACT GAAGTGGTCT CATATTGAAT GGTCACAGAC CGAATATGAA 
GTCTGTGAGA ATGTGGGTTT GTTGCCCTTG GAAATTATCA GAAGGGGATA TTCCATGGAC 
TCGGCCTTTG TGGGTATAAA GGTCAACCAA GTGTCAGCTG CAGTTGGAAA AGATTTCACC 
GTGATTCCAT CTAAACTGAT TCAGTTTGAC CCAGCTTCCA TGATTGGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FREM1 ( XM_017014329.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016869818.1 Homo sapiens FRAS1 related extracellular matrix 1 (FREM1), transcript variant X14, mRNA.

Price & Availability↑

CloneID	OHu98465
Clone ID Related Accession (Same CDS sequence)	XM_017014329.1 , XM_017014329.2
Accession Version	XM_017014329.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5451bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	FRAS1-related extracellular matrix protein 1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017014329.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACTCTC TGAGTTGGGG GGCTGCGAAT GCCGTGCTGC TGCTGCTCCT CCTGGCCTGG 
GCCAGCCCCA CCTTCATCAG CATCAACCGC GGGGTGAGGG TGATGAAGGG CCACTCTGCC 
TTCCTGTCAG GAGATGACCT GAAGTTTGCC ATCCCTAAAG AGAAAGATGC CTGCAAAGTG 
GAAGTTGTGA TGAATGAGCC AATAACCCAG AGGGTTGGGA AACTCACTCC ACAGGTCTTT 
GACTGCCATT TCCTTCCCAA CGAAGTCAAG TATGTTCACA ATGGTTGTCC AATTCTTGAT 
GAAGACACAG TGAAGCTCAG ACTTTACAGA TTTACTGAAA GAGATACCTT CATAGAAACT 
TTTATCCTGT GGGTCTATCT CCTGGAACCA GACTGTAACA TCATCCATAT GAGTAACAAT 
GTGCTGGAGG TGCCTGAATT CAATGGCTTG TCCCAAGCGA TTGATAAAAA TCTGCTCAGA 
TTCGATTATG ATAGGATGGC TAGCCTGGAA TGTACCGTCA GCCTGGACAC TGCGAGAACT 
CGGCTGCCAG CCCATGGCCA GATGGTTCTC GGGGAGCCTC GACCAGAAGA ACCTCGTGGA 
GATCAGCCAC ACAGTTTTTT CCCAGAGTCC CGTATGATGT CTTTTAATGC CTTTCTGGAA 
CTGAGAGCAA AACTGAAGTG TCCAGGTGGG AGCTGTACCC CAGGATTAAA GAAAATAGGA 
AGCCTCAAAG TGAGCTGTGA GGAGTTCCTG CTGATGGGCC TTCGTTATCA GCATCTGGAT 
CCCCCTTCAC CCAACATTGA TTATATCTCC ATCCAACTGG ACCTCACAGA TACCAGGAGC 
AAAATTGTGT ACAAGTCAGA GAGTGCGTGG CTGCCTGTCT ATATCAGAGC TGGAATTCCG 
AATCAGATTC CAAAGGCTGC ATTCATGGCC GTGTTTATTC TGGAAGTGGA TCAGTTCATC 
CTGACCTCCT TGACTACATC AGTTCTGGAC TGTGAAGAAG ATGAGACCCC TAAACCCTTG 
CTGGTGTTCA ACATTACTAA AGCCCCGCTC CAGGGCTATG TGACTCACCT GTTGGATCAC 
ACCAGACCAA TCTCCTCATT CACCTGGAAA GATCTCAGTG ACATGCAGAT CGCCTATCAG 
CCACCAAACA GCAGCCATTC TGAGAGGAGA CATGATGAGG TAGAATTGGA GGTATACGAC 
TTCTTCTTTG AAAGGAGTGC ACCTATGACA GTCCACATCT CCATCAGAAC AGCAGATACA 
AATGCCCCCC GTGTATCCTG GAATACAGGT CTGAGTCTCC TTGAGGGGCA GTCTCGAGCC 
ATCACTTGGG AACAGTTTCA GGTTGTCGAC AATGACGACA TTGGTGCTGT CCGGCTAGTC 
ACCGTTGGTG GCCTGCAGCA TGGATGGCTG ACTTTAAGAG GGGGGAAAGG GTTTCTCTTC 
ACCGTGGCTG ACCTCCAGGC TGGAGTTGTT CGCTATCATC ATGATGACAG CGACTCCACC 
AAAGACTTCG TGGTCTTCCG GATATTTGAT GGCCATCACA GCATCCGTCA CAAATTCCCC 
ATCAACGTCT TGCCCAAAGA TGATAGTCCC CCGTTCCTCA TAACCAATGT TGTGATTGAA 
CTGGAGGAGG GGCAGACCAT CCTGATCCAG GGATCCATGC TGCGAGCTTC AGATGTGGAC 
GCCAGTGATG ACTACATCTT CTTCAATATC ACAAAGCCTC CACAGGCTGG GGAGATCATG 
AAGAAGCCAG GGCCAGGACT GATAGGCTAT CCTGTCCATG GCTTCCTTCA GAGGGATTTG 
TTTAATGGAA TCATTTATTA TCGTCATTTT GGTGGAGAAA TCTTTGAAGA TTCTTTTCAA 
TTTGTCCTGT GGGACAGCCA TGAACCTCCA AATCTTTCAG TGCCACAGGT GGCAACAATC 
CATATAACTC CAGTGGATGA CCAGCTTCCA AAAGAGGCTC CTGGAGTTTC TCGGCATTTG 
GTTGTCAAGG AAACTGAGGT GGCCTATATA ACTAAGAAAC AGCTACATTT TATAGATTCA 
GAATCATATG ACAGGGAGCT GGTCTACACA ATAACTACTC CTCCATTTTT CTCCTTCAGC 
CACAGACACT TGGATGCTGG GAAATTATTT ATGGTGGACA GCATACCAAA AGTAGTTAAG 
AATCCTACGG CCCTGGAGCT GAGGTCATTC ACTCAGCATG CTGTGAACTA TATGAAAGTG 
GCCTACATGC CCCCCATGCA AGACATTGGT CCCCATTGCA GAGATGTCCA GTTCACATTT 
TCTGTCAGTA ACCAACATGG CGGTACTTTG CATGGGATCT GCTTTAACAT TACAATCCTC 
CCAGTGGACA ATCAAGTTCC TGAGGCGTTC ACCAACCCTC TGAAAGTGAC TGAGGGAGGT 
CAAAGCATCA TCAGCACAGA GCACATTCTA ATTTCTGATG CAGATACCAA GCTGGACAAT 
ATTGACCTCT CCCTGCGGGA ATTGCCTCTG CACGGAAGGG TGGAGCTGAA TGGATTTCCT 
CTAAATTCAG GGGGCACATT TTCTTGGGGC GATCTCCATA CCTTAAAAGT TAGGTATCAA 
CATGATGGAA CTGAAGTTCT TCAGGATGAC CTACTCTTGG AGGTCACCGA TGGCACAAAT 
TCAGCAGAAT TTGTACTACA TGTTGAGGTA TTCCCTGTCA ACGATGAGCC ACCAGTCTTA 
AAGGCTGACC TCATGCCTGT CATGAATTGC TCAGAGGGAG GAGAGGTGGT CATCACCTCT 
GAATACATTT TTGCTACTGA TGTGGACAGC GATAACTTGA AGTTGATGTT TGTGATTGCT 
CGCGAACCTC AGCATGGGGT GGTGAGGAGA GCTGGAGTCA CAGTGGATCA GTTCTCTCAG 
AGAGATGTTA TCTCAGAGGC CGTGACATAC AAACACACAG GTGGCGAGAT TGGCCTGATG 
CCTTGTTTTG ACACCATTAC ATTGGTGGTT TCGGATGGAG AGGCTGGCCC TTTTGTGAAT 
GGCTGTTGCT ACAATGGACC CAATCCATCT GTTCCACTTC ATGCGTCCTT TCCAGTGTAT 
GATCTCAACA TCACGGTATA CCCAGTAGAC AACCAGCCAC CTTCCATTGC AATAGGTCCC 
GTGTTTGTTG TAGATGAGGG CTGCTCAACA GCCCTTACTG TTAACCATTT GTCCGCCACT 
GACCCTGACA CTGCAGCAGA TGACTTGGAA TTTGTTTTGG TTTCTCCTCC TCAGTTTGGC 
TACCTCGAAA ATATACTCCC TTCTGTGGGT TTTGAAAAAA GCAATATTGG CATAAGTATA 
GATTCATTTC AGTGGAAAGA CATGAACGCT TTTCACATTA ACTATGTGCA GTCCAGGCAT 
CTGAGGATAG AACCAACTGC CGACCAGTTC ACGGTGTACG TCACAGATGG GAAGCATCAC 
TCCTTGGAGA TACCATTTTC TATTATAATC AACCCCACAA ATGATGAAGC TCCTGACTTT 
GTAGTGCAGA ATATTACTGT GTGTGAGGGT CAGATGAAAG AGCTGGACTC TTCCATCATC 
AGCGCTGTGG ACCTGGACAT TCCCCAGGAT GCCCTGCTGT TCAGCATCAC TCAAAAGCCA 
CGCCATGGCC TCCTCATCGA TAGGGGGTTT AGCAAAGACT TCTCTGAGAA TAAGCAGCCA 
GCCAACCCTC ACCAGAAACA TGCACCTGTT CACAGCTTTT CCATGGAACT CCTCAAGACT 
GGAATGAGGT TGACGTACAT GCATGATGAC TCAGAGAGCC TTGCTGATGA TTTTACAATC 
CAATTGTCAG ATGGGAAACA TAAGATACTT AAAACCATTT CAGTAGAGGT CATCCCAGTT 
AATGATGAAA AACCAATGCT GAGCAAGAAG GCTGAAATTG CAATGAATAT GGGTGAAACT 
CGTATTATTT CCAGTGCTAT TCTTTCAGCC ATAGATGAAG ACTCACCCAG GGAGAAGATT 
TACTATGTAT TTGAAAGGCT TCCCCAAAAT GGGCAACTTC AGCTTAAGAT AGGGAGGGAC 
TGGGTTCCTC TCTCCCCTGG CATGAAATGC ACTCAGGAGG AAGTGGATCT GAACTTGCTG 
AGATACACAC ACACCGGGGC AATGGATTCC CAGAATCAAG ATAGCTTCAC CTTCTACCTT 
TGGGATGGCA ACAACAGGTC CCCTGCTCTT GACTGTCAAA TCACCATCAA GGACATGGAA 
AAAGGTGATA TTGTCATCCT TACAAAACCA CTCGTGGTGT CTAAAGGTGA TAGAGGTTTC 
TTAACAACCA CCACGCTCCT GGCTGTGGAC GGAACAGACA AGCCTGAGGA ACTGCTCTAT 
GTCATCACCT CCCCTCCGCG ATATGGCCAG ATCGAATATG TTCACTATCC TGGAGTTCCC 
ATTACAAACT TCAGCCAAAT GGATGTAGTG GGGCAGACAG TTTGCTATGT ACACAAGAGC 
AAGGTGACTG TCTCCAGTGA CAGATTCAGA TTCATCATCA GCAATGGACT GCGGACCGAG 
CACGGGGTGT TTGAGATCAC ACTGGAGACT GTGGACAGAG CCCTGCCTGT GGTAACCAGG 
AACAAGGGGT TGAGACTGGC CCAAGGGGCC GTGGGCCTGC TTTCCCCTGA CCTCCTTCAG 
CTGACCGACC CTGATACACC TGCGGAGAAC CTCACCTTCC TCTTGGTTCA GCTCCCCCAG 
CATGGCCAGC TCTACCTGTG GGGGACAGGG CTACTTCAAC ACAATTTCAC CCAGCAGGAT 
GTGGACAGCA AGAATGTGGC CTATCGGCAC TCAGGAGGGG ACTCCCAGAC TGACTGCTTT 
ACTTTCATGG CCACAGATGG GACAAACCAA GGCTTTATTG TGAATGGGAG AGTGTGGGAA 
GAACCTGTTT TATTCACCAT TCAGGTAGAC CAATTGGACA AAACAGCTCC TCGTATCACA 
CTCTTGCATT CCCCTTCTCA AGTGGGGCTC CTGAAAAATG GCTGCTACGG GATTTACATC 
ACTTCCCGCG TGTTGAAGGC ATCAGACCCT GACACTGAGG ACGATCAGAT CATCTTTAAA 
ATTCTACAAG GCCCAAAACA TGGACATCTG GAGAACACAA CAACAGGTGA ATTTATCCAT 
GAGAAATTTA GCCAAAAGGA CTTAAACAGT AAGACTATTC TTTACATCAT AAACCCATCT 
TTGGAAGTAA ATTCAGATAC CGTGGAATTT CAAATCATGG ACCCCACAGG GAACTCGGCC 
ACTCCTCAAA TTTTGGAACT GAAGTGGTCT CATATTGAAT GGTCACAGAC CGAATATGAA 
GTCTGTGAGA ATGTGGGTTT GTTGCCCTTG GAAATTATCA GAAGGGGATA TTCCATGGAC 
TCGGCCTTTG TGGGTATAAA GGTCAACCAA GTGTCAGCTG CAGTTGGAAA AGATTTCACC 
GTGATTCCAT CTAAACTGAT TCAGTTTGAC CCAGCTTCCA TGATTGGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016869818.1
CDS	787..6237
Translation	MNSLSWGAANAVLLLLLLAWASPTFISINRGVRVMKGHSAFLSGDDLKFAIPKEKDACKVEVVMNEPITQRVGKLTPQVFDCHFLPNEVKYVHNGCPILDEDTVKLRLYRFTERDTFIETFILWVYLLEPDCNIIHMSNNVLEVPEFNGLSQAIDKNLLRFDYDRMASLECTVSLDTARTRLPAHGQMVLGEPRPEEPRGDQPHSFFPESRMMSFNAFLELRAKLKCPGGSCTPGLKKIGSLKVSCEEFLLMGLRYQHLDPPSPNIDYISIQLDLTDTRSKIVYKSESAWLPVYIRAGIPNQIPKAAFMAVFILEVDQFILTSLTTSVLDCEEDETPKPLLVFNITKAPLQGYVTHLLDHTRPISSFTWKDLSDMQIAYQPPNSSHSERRHDEVELEVYDFFFERSAPMTVHISIRTADTNAPRVSWNTGLSLLEGQSRAITWEQFQVVDNDDIGAVRLVTVGGLQHGWLTLRGGKGFLFTVADLQAGVVRYHHDDSDSTKDFVVFRIFDGHHSIRHKFPINVLPKDDSPPFLITNVVIELEEGQTILIQGSMLRASDVDASDDYIFFNITKPPQAGEIMKKPGPGLIGYPVHGFLQRDLFNGIIYYRHFGGEIFEDSFQFVLWDSHEPPNLSVPQVATIHITPVDDQLPKEAPGVSRHLVVKETEVAYITKKQLHFIDSESYDRELVYTITTPPFFSFSHRHLDAGKLFMVDSIPKVVKNPTALELRSFTQHAVNYMKVAYMPPMQDIGPHCRDVQFTFSVSNQHGGTLHGICFNITILPVDNQVPEAFTNPLKVTEGGQSIISTEHILISDADTKLDNIDLSLRELPLHGRVELNGFPLNSGGTFSWGDLHTLKVRYQHDGTEVLQDDLLLEVTDGTNSAEFVLHVEVFPVNDEPPVLKADLMPVMNCSEGGEVVITSEYIFATDVDSDNLKLMFVIAREPQHGVVRRAGVTVDQFSQRDVISEAVTYKHTGGEIGLMPCFDTITLVVSDGEAGPFVNGCCYNGPNPSVPLHASFPVYDLNITVYPVDNQPPSIAIGPVFVVDEGCSTALTVNHLSATDPDTAADDLEFVLVSPPQFGYLENILPSVGFEKSNIGISIDSFQWKDMNAFHINYVQSRHLRIEPTADQFTVYVTDGKHHSLEIPFSIIINPTNDEAPDFVVQNITVCEGQMKELDSSIISAVDLDIPQDALLFSITQKPRHGLLIDRGFSKDFSENKQPANPHQKHAPVHSFSMELLKTGMRLTYMHDDSESLADDFTIQLSDGKHKILKTISVEVIPVNDEKPMLSKKAEIAMNMGETRIISSAILSAIDEDSPREKIYYVFERLPQNGQLQLKIGRDWVPLSPGMKCTQEEVDLNLLRYTHTGAMDSQNQDSFTFYLWDGNNRSPALDCQITIKDMEKGDIVILTKPLVVSKGDRGFLTTTTLLAVDGTDKPEELLYVITSPPRYGQIEYVHYPGVPITNFSQMDVVGQTVCYVHKSKVTVSSDRFRFIISNGLRTEHGVFEITLETVDRALPVVTRNKGLRLAQGAVGLLSPDLLQLTDPDTPAENLTFLLVQLPQHGQLYLWGTGLLQHNFTQQDVDSKNVAYRHSGGDSQTDCFTFMATDGTNQGFIVNGRVWEEPVLFTIQVDQLDKTAPRITLLHSPSQVGLLKNGCYGIYITSRVLKASDPDTEDDQIIFKILQGPKHGHLENTTTGEFIHEKFSQKDLNSKTILYIINPSLEVNSDTVEFQIMDPTGNSATPQILELKWSHIEWSQTEYEVCENVGLLPLEIIRRGYSMDSAFVGIKVNQVSAAVGKDFTVIPSKLIQFDPASMIG

Target ORF information:

RefSeq Version	XM_017014329.2
Organism	Homo sapiens(human)
Definition	Homo sapiens FRAS1 related extracellular matrix 1 (FREM1), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017014329.2

ORF Insert Sequence:

ATGAACTCTC TGAGTTGGGG GGCTGCGAAT GCCGTGCTGC TGCTGCTCCT CCTGGCCTGG 
GCCAGCCCCA CCTTCATCAG CATCAACCGC GGGGTGAGGG TGATGAAGGG CCACTCTGCC 
TTCCTGTCAG GAGATGACCT GAAGTTTGCC ATCCCTAAAG AGAAAGATGC CTGCAAAGTG 
GAAGTTGTGA TGAATGAGCC AATAACCCAG AGGGTTGGGA AACTCACTCC ACAGGTCTTT 
GACTGCCATT TCCTTCCCAA CGAAGTCAAG TATGTTCACA ATGGTTGTCC AATTCTTGAT 
GAAGACACAG TGAAGCTCAG ACTTTACAGA TTTACTGAAA GAGATACCTT CATAGAAACT 
TTTATCCTGT GGGTCTATCT CCTGGAACCA GACTGTAACA TCATCCATAT GAGTAACAAT 
GTGCTGGAGG TGCCTGAATT CAATGGCTTG TCCCAAGCGA TTGATAAAAA TCTGCTCAGA 
TTCGATTATG ATAGGATGGC TAGCCTGGAA TGTACCGTCA GCCTGGACAC TGCGAGAACT 
CGGCTGCCAG CCCATGGCCA GATGGTTCTC GGGGAGCCTC GACCAGAAGA ACCTCGTGGA 
GATCAGCCAC ACAGTTTTTT CCCAGAGTCC CGTATGATGT CTTTTAATGC CTTTCTGGAA 
CTGAGAGCAA AACTGAAGTG TCCAGGTGGG AGCTGTACCC CAGGATTAAA GAAAATAGGA 
AGCCTCAAAG TGAGCTGTGA GGAGTTCCTG CTGATGGGCC TTCGTTATCA GCATCTGGAT 
CCCCCTTCAC CCAACATTGA TTATATCTCC ATCCAACTGG ACCTCACAGA TACCAGGAGC 
AAAATTGTGT ACAAGTCAGA GAGTGCGTGG CTGCCTGTCT ATATCAGAGC TGGAATTCCG 
AATCAGATTC CAAAGGCTGC ATTCATGGCC GTGTTTATTC TGGAAGTGGA TCAGTTCATC 
CTGACCTCCT TGACTACATC AGTTCTGGAC TGTGAAGAAG ATGAGACCCC TAAACCCTTG 
CTGGTGTTCA ACATTACTAA AGCCCCGCTC CAGGGCTATG TGACTCACCT GTTGGATCAC 
ACCAGACCAA TCTCCTCATT CACCTGGAAA GATCTCAGTG ACATGCAGAT CGCCTATCAG 
CCACCAAACA GCAGCCATTC TGAGAGGAGA CATGATGAGG TAGAATTGGA GGTATACGAC 
TTCTTCTTTG AAAGGAGTGC ACCTATGACA GTCCACATCT CCATCAGAAC AGCAGATACA 
AATGCCCCCC GTGTATCCTG GAATACAGGT CTGAGTCTCC TTGAGGGGCA GTCTCGAGCC 
ATCACTTGGG AACAGTTTCA GGTTGTCGAC AATGACGACA TTGGTGCTGT CCGGCTAGTC 
ACCGTTGGTG GCCTGCAGCA TGGATGGCTG ACTTTAAGAG GGGGGAAAGG GTTTCTCTTC 
ACCGTGGCTG ACCTCCAGGC TGGAGTTGTT CGCTATCATC ATGATGACAG CGACTCCACC 
AAAGACTTCG TGGTCTTCCG GATATTTGAT GGCCATCACA GCATCCGTCA CAAATTCCCC 
ATCAACGTCT TGCCCAAAGA TGATAGTCCC CCGTTCCTCA TAACCAATGT TGTGATTGAA 
CTGGAGGAGG GGCAGACCAT CCTGATCCAG GGATCCATGC TGCGAGCTTC AGATGTGGAC 
GCCAGTGATG ACTACATCTT CTTCAATATC ACAAAGCCTC CACAGGCTGG GGAGATCATG 
AAGAAGCCAG GGCCAGGACT GATAGGCTAT CCTGTCCATG GCTTCCTTCA GAGGGATTTG 
TTTAATGGAA TCATTTATTA TCGTCATTTT GGTGGAGAAA TCTTTGAAGA TTCTTTTCAA 
TTTGTCCTGT GGGACAGCCA TGAACCTCCA AATCTTTCAG TGCCACAGGT GGCAACAATC 
CATATAACTC CAGTGGATGA CCAGCTTCCA AAAGAGGCTC CTGGAGTTTC TCGGCATTTG 
GTTGTCAAGG AAACTGAGGT GGCCTATATA ACTAAGAAAC AGCTACATTT TATAGATTCA 
GAATCATATG ACAGGGAGCT GGTCTACACA ATAACTACTC CTCCATTTTT CTCCTTCAGC 
CACAGACACT TGGATGCTGG GAAATTATTT ATGGTGGACA GCATACCAAA AGTAGTTAAG 
AATCCTACGG CCCTGGAGCT GAGGTCATTC ACTCAGCATG CTGTGAACTA TATGAAAGTG 
GCCTACATGC CCCCCATGCA AGACATTGGT CCCCATTGCA GAGATGTCCA GTTCACATTT 
TCTGTCAGTA ACCAACATGG CGGTACTTTG CATGGGATCT GCTTTAACAT TACAATCCTC 
CCAGTGGACA ATCAAGTTCC TGAGGCGTTC ACCAACCCTC TGAAAGTGAC TGAGGGAGGT 
CAAAGCATCA TCAGCACAGA GCACATTCTA ATTTCTGATG CAGATACCAA GCTGGACAAT 
ATTGACCTCT CCCTGCGGGA ATTGCCTCTG CACGGAAGGG TGGAGCTGAA TGGATTTCCT 
CTAAATTCAG GGGGCACATT TTCTTGGGGC GATCTCCATA CCTTAAAAGT TAGGTATCAA 
CATGATGGAA CTGAAGTTCT TCAGGATGAC CTACTCTTGG AGGTCACCGA TGGCACAAAT 
TCAGCAGAAT TTGTACTACA TGTTGAGGTA TTCCCTGTCA ACGATGAGCC ACCAGTCTTA 
AAGGCTGACC TCATGCCTGT CATGAATTGC TCAGAGGGAG GAGAGGTGGT CATCACCTCT 
GAATACATTT TTGCTACTGA TGTGGACAGC GATAACTTGA AGTTGATGTT TGTGATTGCT 
CGCGAACCTC AGCATGGGGT GGTGAGGAGA GCTGGAGTCA CAGTGGATCA GTTCTCTCAG 
AGAGATGTTA TCTCAGAGGC CGTGACATAC AAACACACAG GTGGCGAGAT TGGCCTGATG 
CCTTGTTTTG ACACCATTAC ATTGGTGGTT TCGGATGGAG AGGCTGGCCC TTTTGTGAAT 
GGCTGTTGCT ACAATGGACC CAATCCATCT GTTCCACTTC ATGCGTCCTT TCCAGTGTAT 
GATCTCAACA TCACGGTATA CCCAGTAGAC AACCAGCCAC CTTCCATTGC AATAGGTCCC 
GTGTTTGTTG TAGATGAGGG CTGCTCAACA GCCCTTACTG TTAACCATTT GTCCGCCACT 
GACCCTGACA CTGCAGCAGA TGACTTGGAA TTTGTTTTGG TTTCTCCTCC TCAGTTTGGC 
TACCTCGAAA ATATACTCCC TTCTGTGGGT TTTGAAAAAA GCAATATTGG CATAAGTATA 
GATTCATTTC AGTGGAAAGA CATGAACGCT TTTCACATTA ACTATGTGCA GTCCAGGCAT 
CTGAGGATAG AACCAACTGC CGACCAGTTC ACGGTGTACG TCACAGATGG GAAGCATCAC 
TCCTTGGAGA TACCATTTTC TATTATAATC AACCCCACAA ATGATGAAGC TCCTGACTTT 
GTAGTGCAGA ATATTACTGT GTGTGAGGGT CAGATGAAAG AGCTGGACTC TTCCATCATC 
AGCGCTGTGG ACCTGGACAT TCCCCAGGAT GCCCTGCTGT TCAGCATCAC TCAAAAGCCA 
CGCCATGGCC TCCTCATCGA TAGGGGGTTT AGCAAAGACT TCTCTGAGAA TAAGCAGCCA 
GCCAACCCTC ACCAGAAACA TGCACCTGTT CACAGCTTTT CCATGGAACT CCTCAAGACT 
GGAATGAGGT TGACGTACAT GCATGATGAC TCAGAGAGCC TTGCTGATGA TTTTACAATC 
CAATTGTCAG ATGGGAAACA TAAGATACTT AAAACCATTT CAGTAGAGGT CATCCCAGTT 
AATGATGAAA AACCAATGCT GAGCAAGAAG GCTGAAATTG CAATGAATAT GGGTGAAACT 
CGTATTATTT CCAGTGCTAT TCTTTCAGCC ATAGATGAAG ACTCACCCAG GGAGAAGATT 
TACTATGTAT TTGAAAGGCT TCCCCAAAAT GGGCAACTTC AGCTTAAGAT AGGGAGGGAC 
TGGGTTCCTC TCTCCCCTGG CATGAAATGC ACTCAGGAGG AAGTGGATCT GAACTTGCTG 
AGATACACAC ACACCGGGGC AATGGATTCC CAGAATCAAG ATAGCTTCAC CTTCTACCTT 
TGGGATGGCA ACAACAGGTC CCCTGCTCTT GACTGTCAAA TCACCATCAA GGACATGGAA 
AAAGGTGATA TTGTCATCCT TACAAAACCA CTCGTGGTGT CTAAAGGTGA TAGAGGTTTC 
TTAACAACCA CCACGCTCCT GGCTGTGGAC GGAACAGACA AGCCTGAGGA ACTGCTCTAT 
GTCATCACCT CCCCTCCGCG ATATGGCCAG ATCGAATATG TTCACTATCC TGGAGTTCCC 
ATTACAAACT TCAGCCAAAT GGATGTAGTG GGGCAGACAG TTTGCTATGT ACACAAGAGC 
AAGGTGACTG TCTCCAGTGA CAGATTCAGA TTCATCATCA GCAATGGACT GCGGACCGAG 
CACGGGGTGT TTGAGATCAC ACTGGAGACT GTGGACAGAG CCCTGCCTGT GGTAACCAGG 
AACAAGGGGT TGAGACTGGC CCAAGGGGCC GTGGGCCTGC TTTCCCCTGA CCTCCTTCAG 
CTGACCGACC CTGATACACC TGCGGAGAAC CTCACCTTCC TCTTGGTTCA GCTCCCCCAG 
CATGGCCAGC TCTACCTGTG GGGGACAGGG CTACTTCAAC ACAATTTCAC CCAGCAGGAT 
GTGGACAGCA AGAATGTGGC CTATCGGCAC TCAGGAGGGG ACTCCCAGAC TGACTGCTTT 
ACTTTCATGG CCACAGATGG GACAAACCAA GGCTTTATTG TGAATGGGAG AGTGTGGGAA 
GAACCTGTTT TATTCACCAT TCAGGTAGAC CAATTGGACA AAACAGCTCC TCGTATCACA 
CTCTTGCATT CCCCTTCTCA AGTGGGGCTC CTGAAAAATG GCTGCTACGG GATTTACATC 
ACTTCCCGCG TGTTGAAGGC ATCAGACCCT GACACTGAGG ACGATCAGAT CATCTTTAAA 
ATTCTACAAG GCCCAAAACA TGGACATCTG GAGAACACAA CAACAGGTGA ATTTATCCAT 
GAGAAATTTA GCCAAAAGGA CTTAAACAGT AAGACTATTC TTTACATCAT AAACCCATCT 
TTGGAAGTAA ATTCAGATAC CGTGGAATTT CAAATCATGG ACCCCACAGG GAACTCGGCC 
ACTCCTCAAA TTTTGGAACT GAAGTGGTCT CATATTGAAT GGTCACAGAC CGAATATGAA 
GTCTGTGAGA ATGTGGGTTT GTTGCCCTTG GAAATTATCA GAAGGGGATA TTCCATGGAC 
TCGGCCTTTG TGGGTATAAA GGTCAACCAA GTGTCAGCTG CAGTTGGAAA AGATTTCACC 
GTGATTCCAT CTAAACTGAT TCAGTTTGAC CCAGCTTCCA TGATTGGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FREM1 ( XM_017014320.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016869809.1 Homo sapiens FRAS1 related extracellular matrix 1 (FREM1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OHu98459
Clone ID Related Accession (Same CDS sequence)	XM_017014320.1 , XM_017014318.1 , XM_017014316.1 , XM_017014316.2 , XM_017014317.1 , XM_017014319.2 , XM_017014321.2 , XM_017014323.1 , XM_017014322.1 , XM_017014321.1 , XM_017014320.2 , XM_017014319.1
Accession Version	XM_017014320.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6567bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	FRAS1-related extracellular matrix protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACTCTC TGAGTTGGGG GGCTGCGAAT GCCGTGCTGC TGCTGCTCCT CCTGGCCTGG 
GCCAGCCCCA CCTTCATCAG CATCAACCGC GGGGTGAGGG TGATGAAGGG CCACTCTGCC 
TTCCTGTCAG GAGATGACCT GAAGTTTGCC ATCCCTAAAG AGAAAGATGC CTGCAAAGTG 
GAAGTTGTGA TGAATGAGCC AATAACCCAG AGGGTTGGGA AACTCACTCC ACAGGTCTTT 
GACTGCCATT TCCTTCCCAA CGAAGTCAAG TATGTTCACA ATGGTTGTCC AATTCTTGAT 
GAAGACACAG TGAAGCTCAG ACTTTACAGA TTTACTGAAA GAGATACCTT CATAGAAACT 
TTTATCCTGT GGGTCTATCT CCTGGAACCA GACTGTAACA TCATCCATAT GAGTAACAAT 
GTGCTGGAGG TGCCTGAATT CAATGGCTTG TCCCAAGCGA TTGATAAAAA TCTGCTCAGA 
TTCGATTATG ATAGGATGGC TAGCCTGGAA TGTACCGTCA GCCTGGACAC TGCGAGAACT 
CGGCTGCCAG CCCATGGCCA GATGGTTCTC GGGGAGCCTC GACCAGAAGA ACCTCGTGGA 
GATCAGCCAC ACAGTTTTTT CCCAGAGTCC CGTATGATGT CTTTTAATGC CTTTCTGGAA 
CTGAGAGCAA AACTGAAGTG TCCAGGTGGG AGCTGTACCC CAGGATTAAA GAAAATAGGA 
AGCCTCAAAG TGAGCTGTGA GGAGTTCCTG CTGATGGGCC TTCGTTATCA GCATCTGGAT 
CCCCCTTCAC CCAACATTGA TTATATCTCC ATCCAACTGG ACCTCACAGA TACCAGGAGC 
AAAATTGTGT ACAAGTCAGA GAGTGCGTGG CTGCCTGTCT ATATCAGAGC TGGAATTCCG 
AATCAGATTC CAAAGGCTGC ATTCATGGCC GTGTTTATTC TGGAAGTGGA TCAGTTCATC 
CTGACCTCCT TGACTACATC AGTTCTGGAC TGTGAAGAAG ATGAGACCCC TAAACCCTTG 
CTGGTGTTCA ACATTACTAA AGCCCCGCTC CAGGGCTATG TGACTCACCT GTTGGATCAC 
ACCAGACCAA TCTCCTCATT CACCTGGAAA GATCTCAGTG ACATGCAGAT CGCCTATCAG 
CCACCAAACA GCAGCCATTC TGAGAGGAGA CATGATGAGG TAGAATTGGA GGTATACGAC 
TTCTTCTTTG AAAGGAGTGC ACCTATGACA GTCCACATCT CCATCAGAAC AGCAGATACA 
AATGCCCCCC GTGTATCCTG GAATACAGGT CTGAGTCTCC TTGAGGGGCA GTCTCGAGCC 
ATCACTTGGG AACAGTTTCA GGTTGTCGAC AATGACGACA TTGGTGCTGT CCGGCTAGTC 
ACCGTTGGTG GCCTGCAGCA TGGATGGCTG ACTTTAAGAG GGGGGAAAGG GTTTCTCTTC 
ACCGTGGCTG ACCTCCAGGC TGGAGTTGTT CGCTATCATC ATGATGACAG CGACTCCACC 
AAAGACTTCG TGGTCTTCCG GATATTTGAT GGCCATCACA GCATCCGTCA CAAATTCCCC 
ATCAACGTCT TGCCCAAAGA TGATAGTCCC CCGTTCCTCA TAACCAATGT TGTGATTGAA 
CTGGAGGAGG GGCAGACCAT CCTGATCCAG GGATCCATGC TGCGAGCTTC AGATGTGGAC 
GCCAGTGATG ACTACATCTT CTTCAATATC ACAAAGCCTC CACAGGCTGG GGAGATCATG 
AAGAAGCCAG GGCCAGGACT GATAGGCTAT CCTGTCCATG GCTTCCTTCA GAGGGATTTG 
TTTAATGGAA TCATTTATTA TCGTCATTTT GGTGGAGAAA TCTTTGAAGA TTCTTTTCAA 
TTTGTCCTGT GGGACAGCCA TGAACCTCCA AATCTTTCAG TGCCACAGGT GGCAACAATC 
CATATAACTC CAGTGGATGA CCAGCTTCCA AAAGAGGCTC CTGGAGTTTC TCGGCATTTG 
GTTGTCAAGG AAACTGAGGT GGCCTATATA ACTAAGAAAC AGCTACATTT TATAGATTCA 
GAATCATATG ACAGGGAGCT GGTCTACACA ATAACTACTC CTCCATTTTT CTCCTTCAGC 
CACAGACACT TGGATGCTGG GAAATTATTT ATGGTGGACA GCATACCAAA AGTAGTTAAG 
AATCCTACGG CCCTGGAGCT GAGGTCATTC ACTCAGCATG CTGTGAACTA TATGAAAGTG 
GCCTACATGC CCCCCATGCA AGACATTGGT CCCCATTGCA GAGATGTCCA GTTCACATTT 
TCTGTCAGTA ACCAACATGG CGGTACTTTG CATGGGATCT GCTTTAACAT TACAATCCTC 
CCAGTGGACA ATCAAGTTCC TGAGGCGTTC ACCAACCCTC TGAAAGTGAC TGAGGGAGGT 
CAAAGCATCA TCAGCACAGA GCACATTCTA ATTTCTGATG CAGATACCAA GCTGGACAAT 
ATTGACCTCT CCCTGCGGGA ATTGCCTCTG CACGGAAGGG TGGAGCTGAA TGGATTTCCT 
CTAAATTCAG GGGGCACATT TTCTTGGGGC GATCTCCATA CCTTAAAAGT TAGGTATCAA 
CATGATGGAA CTGAAGTTCT TCAGGATGAC CTACTCTTGG AGGTCACCGA TGGCACAAAT 
TCAGCAGAAT TTGTACTACA TGTTGAGGTA TTCCCTGTCA ACGATGAGCC ACCAGTCTTA 
AAGGCTGACC TCATGCCTGT CATGAATTGC TCAGAGGGAG GAGAGGTGGT CATCACCTCT 
GAATACATTT TTGCTACTGA TGTGGACAGC GATAACTTGA AGTTGATGTT TGTGATTGCT 
CGCGAACCTC AGCATGGGGT GGTGAGGAGA GCTGGAGTCA CAGTGGATCA GTTCTCTCAG 
AGAGATGTTA TCTCAGAGGC CGTGACATAC AAACACACAG GTGGCGAGAT TGGCCTGATG 
CCTTGTTTTG ACACCATTAC ATTGGTGGTT TCGGATGGAG AGGCTGGCCC TTTTGTGAAT 
GGCTGTTGCT ACAATGGACC CAATCCATCT GTTCCACTTC ATGCGTCCTT TCCAGTGTAT 
GATCTCAACA TCACGGTATA CCCAGTAGAC AACCAGCCAC CTTCCATTGC AATAGGTCCC 
GTGTTTGTTG TAGATGAGGG CTGCTCAACA GCCCTTACTG TTAACCATTT GTCCGCCACT 
GACCCTGACA CTGCAGCAGA TGACTTGGAA TTTGTTTTGG TTTCTCCTCC TCAGTTTGGC 
TACCTCGAAA ATATACTCCC TTCTGTGGGT TTTGAAAAAA GCAATATTGG CATAAGTATA 
GATTCATTTC AGTGGAAAGA CATGAACGCT TTTCACATTA ACTATGTGCA GTCCAGGCAT 
CTGAGGATAG AACCAACTGC CGACCAGTTC ACGGTGTACG TCACAGATGG GAAGCATCAC 
TCCTTGGAGA TACCATTTTC TATTATAATC AACCCCACAA ATGATGAAGC TCCTGACTTT 
GTAGTGCAGA ATATTACTGT GTGTGAGGGT CAGATGAAAG AGCTGGACTC TTCCATCATC 
AGCGCTGTGG ACCTGGACAT TCCCCAGGAT GCCCTGCTGT TCAGCATCAC TCAAAAGCCA 
CGCCATGGCC TCCTCATCGA TAGGGGGTTT AGCAAAGACT TCTCTGAGAA TAAGCAGCCA 
GCCAACCCTC ACCAGAAACA TGCACCTGTT CACAGCTTTT CCATGGAACT CCTCAAGACT 
GGAATGAGGT TGACGTACAT GCATGATGAC TCAGAGAGCC TTGCTGATGA TTTTACAATC 
CAATTGTCAG ATGGGAAACA TAAGATACTT AAAACCATTT CAGTAGAGGT CATCCCAGTT 
AATGATGAAA AACCAATGCT GAGCAAGAAG GCTGAAATTG CAATGAATAT GGGTGAAACT 
CGTATTATTT CCAGTGCTAT TCTTTCAGCC ATAGATGAAG ACTCACCCAG GGAGAAGATT 
TACTATGTAT TTGAAAGGCT TCCCCAAAAT GGGCAACTTC AGCTTAAGAT AGGGAGGGAC 
TGGGTTCCTC TCTCCCCTGG CATGAAATGC ACTCAGGAGG AAGTGGATCT GAACTTGCTG 
AGATACACAC ACACCGGGGC AATGGATTCC CAGAATCAAG ATAGCTTCAC CTTCTACCTT 
TGGGATGGCA ACAACAGGTC CCCTGCTCTT GACTGTCAAA TCACCATCAA GGACATGGAA 
AAAGGTGATA TTGTCATCCT TACAAAACCA CTCGTGGTGT CTAAAGGTGA TAGAGGTTTC 
TTAACAACCA CCACGCTCCT GGCTGTGGAC GGAACAGACA AGCCTGAGGA ACTGCTCTAT 
GTCATCACCT CCCCTCCGCG ATATGGCCAG ATCGAATATG TTCACTATCC TGGAGTTCCC 
ATTACAAACT TCAGCCAAAT GGATGTAGTG GGGCAGACAG TTTGCTATGT ACACAAGAGC 
AAGGTGACTG TCTCCAGTGA CAGATTCAGA TTCATCATCA GCAATGGACT GCGGACCGAG 
CACGGGGTGT TTGAGATCAC ACTGGAGACT GTGGACAGAG CCCTGCCTGT GGTAACCAGG 
AACAAGGGGT TGAGACTGGC CCAAGGGGCC GTGGGCCTGC TTTCCCCTGA CCTCCTTCAG 
CTGACCGACC CTGATACACC TGCGGAGAAC CTCACCTTCC TCTTGGTTCA GCTCCCCCAG 
CATGGCCAGC TCTACCTGTG GGGGACAGGG CTACTTCAAC ACAATTTCAC CCAGCAGGAT 
GTGGACAGCA AGAATGTGGC CTATCGGCAC TCAGGAGGGG ACTCCCAGAC TGACTGCTTT 
ACTTTCATGG CCACAGATGG GACAAACCAA GGCTTTATTG TGAATGGGAG AGTGTGGGAA 
GAACCTGTTT TATTCACCAT TCAGGTAGAC CAATTGGACA AAACAGCTCC TCGTATCACA 
CTCTTGCATT CCCCTTCTCA AGTGGGGCTC CTGAAAAATG GCTGCTACGG GATTTACATC 
ACTTCCCGCG TGTTGAAGGC ATCAGACCCT GACACTGAGG ACGATCAGAT CATCTTTAAA 
ATTCTACAAG GCCCAAAACA TGGACATCTG GAGAACACAA CAACAGGTGA ATTTATCCAT 
GAGAAATTTA GCCAAAAGGA CTTAAACAGT AAGACTATTC TTTACATCAT AAACCCATCT 
TTGGAAGTAA ATTCAGATAC CGTGGAATTT CAAATCATGG ACCCCACAGG GAACTCGGCC 
ACTCCTCAAA TTTTGGAACT GAAGTGGTCT CATATTGAAT GGTCACAGAC CGAATATGAA 
GTCTGTGAGA ATGTGGGTTT GTTGCCCTTG GAAATTATCA GAAGGGGATA TTCCATGGAC 
TCGGCCTTTG TGGGTATAAA GGTCAACCAA GTGTCAGCTG CAGTTGGAAA AGATTTCACC 
GTGATTCCAT CTAAACTGAT TCAGTTTGAC CCAGGAATGT CAACTAAGAT GTGGAATATA 
GCAATTACCT ATGACGGATT AGAGGAAGAT GATGAGGTCT TTGAAGTAAT TCTGAACTCC 
CCTGTGAATG CAGTTCTTGG CACAAAGACA AAAGCTGCAG TGAAAATTTT GGACTCAAAA 
GGAGGACAAT GCCATCCTTC ATATTCCTCC AACCAAAGCA AGCACAGCAC ATGGGAGAAG 
GGCATTTGGC ATCTGCTGCC CCCAGGGTCT TCCTCATCCA CCACTTCTGG TTCCTTTCAT 
CTGGAAAGAA GACCTCTTCC ATCTTCCATG CAGCTAGCAG TCATCAGGGG AGACACCCTG 
CGGGGCTTTG ATTCTACAGA TCTTTCTCAA AGGAAGCTTA GGACCCGTGG GAATGGCAAA 
ACAGTTCGTC CATCCTCTGT TTATAGAAAT GGAACAGACA TCATCTATAA TTATCATGGG 
ATAGTTTCCT TGAAACTGGA GGATGACAGT TTCCCAACTC ACAAAAGGAA GGCCAAAGTA 
TCCATCATTA GTCAGCCACA AAAGACAATC AAAGTGGCAG AACTGCCTCA AGCAGATAAG 
GTGGAATCCA CAACTGACTC ACACTTCCCC AGACAGGACC AGTTGCCCTC ATTTCCAAAG 
AACTGCACTC TGGAATTAAA GGGACTCTTC CATTTTGAAG AAGGCATCCA GAAGCTGTAT 
CAGTGCAATG GGATCGCCTG GAAAGCCTGG AGTCCCCAAA CCAAGGATGT GGAAGACAAA 
TCCTGTCCAG CCGGGTGGCA CCAGCACTCA GGCTACTGTC ACATCTTGAT CACAGAGCAG 
AAAGGCACCT GGAATGCGGC TGCCCAAGCT TGCAGGGAAC AATACCTGGG CAACCTTGTA 
ACTGTATTCT CCAGGCAGCA CATGCGGTGG CTCTGGGACA TTGGTGGGAG AAAGTCCTTT 
TGGATAGGTT TGAACGACCA AGTGCATGCT GGCCACTGGG AGTGGATCGG TGGTGAACCT 
GTTGCCTTCA CCAATGGGAG AAGAGGGCCC TCTCAACGCT CCAAGCTTGG AAAGAGCTGT 
GTTTTGGTTC AAAGACAAGG GAAATGGCAA ACAAAAGACT GTAGGAGAGC CAAACCTCAT 
AATTATGTGT GTTCCAGAAA ACTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016869809.1
CDS	1194..7760
Translation	MNSLSWGAANAVLLLLLLAWASPTFISINRGVRVMKGHSAFLSGDDLKFAIPKEKDACKVEVVMNEPITQRVGKLTPQVFDCHFLPNEVKYVHNGCPILDEDTVKLRLYRFTERDTFIETFILWVYLLEPDCNIIHMSNNVLEVPEFNGLSQAIDKNLLRFDYDRMASLECTVSLDTARTRLPAHGQMVLGEPRPEEPRGDQPHSFFPESRMMSFNAFLELRAKLKCPGGSCTPGLKKIGSLKVSCEEFLLMGLRYQHLDPPSPNIDYISIQLDLTDTRSKIVYKSESAWLPVYIRAGIPNQIPKAAFMAVFILEVDQFILTSLTTSVLDCEEDETPKPLLVFNITKAPLQGYVTHLLDHTRPISSFTWKDLSDMQIAYQPPNSSHSERRHDEVELEVYDFFFERSAPMTVHISIRTADTNAPRVSWNTGLSLLEGQSRAITWEQFQVVDNDDIGAVRLVTVGGLQHGWLTLRGGKGFLFTVADLQAGVVRYHHDDSDSTKDFVVFRIFDGHHSIRHKFPINVLPKDDSPPFLITNVVIELEEGQTILIQGSMLRASDVDASDDYIFFNITKPPQAGEIMKKPGPGLIGYPVHGFLQRDLFNGIIYYRHFGGEIFEDSFQFVLWDSHEPPNLSVPQVATIHITPVDDQLPKEAPGVSRHLVVKETEVAYITKKQLHFIDSESYDRELVYTITTPPFFSFSHRHLDAGKLFMVDSIPKVVKNPTALELRSFTQHAVNYMKVAYMPPMQDIGPHCRDVQFTFSVSNQHGGTLHGICFNITILPVDNQVPEAFTNPLKVTEGGQSIISTEHILISDADTKLDNIDLSLRELPLHGRVELNGFPLNSGGTFSWGDLHTLKVRYQHDGTEVLQDDLLLEVTDGTNSAEFVLHVEVFPVNDEPPVLKADLMPVMNCSEGGEVVITSEYIFATDVDSDNLKLMFVIAREPQHGVVRRAGVTVDQFSQRDVISEAVTYKHTGGEIGLMPCFDTITLVVSDGEAGPFVNGCCYNGPNPSVPLHASFPVYDLNITVYPVDNQPPSIAIGPVFVVDEGCSTALTVNHLSATDPDTAADDLEFVLVSPPQFGYLENILPSVGFEKSNIGISIDSFQWKDMNAFHINYVQSRHLRIEPTADQFTVYVTDGKHHSLEIPFSIIINPTNDEAPDFVVQNITVCEGQMKELDSSIISAVDLDIPQDALLFSITQKPRHGLLIDRGFSKDFSENKQPANPHQKHAPVHSFSMELLKTGMRLTYMHDDSESLADDFTIQLSDGKHKILKTISVEVIPVNDEKPMLSKKAEIAMNMGETRIISSAILSAIDEDSPREKIYYVFERLPQNGQLQLKIGRDWVPLSPGMKCTQEEVDLNLLRYTHTGAMDSQNQDSFTFYLWDGNNRSPALDCQITIKDMEKGDIVILTKPLVVSKGDRGFLTTTTLLAVDGTDKPEELLYVITSPPRYGQIEYVHYPGVPITNFSQMDVVGQTVCYVHKSKVTVSSDRFRFIISNGLRTEHGVFEITLETVDRALPVVTRNKGLRLAQGAVGLLSPDLLQLTDPDTPAENLTFLLVQLPQHGQLYLWGTGLLQHNFTQQDVDSKNVAYRHSGGDSQTDCFTFMATDGTNQGFIVNGRVWEEPVLFTIQVDQLDKTAPRITLLHSPSQVGLLKNGCYGIYITSRVLKASDPDTEDDQIIFKILQGPKHGHLENTTTGEFIHEKFSQKDLNSKTILYIINPSLEVNSDTVEFQIMDPTGNSATPQILELKWSHIEWSQTEYEVCENVGLLPLEIIRRGYSMDSAFVGIKVNQVSAAVGKDFTVIPSKLIQFDPGMSTKMWNIAITYDGLEEDDEVFEVILNSPVNAVLGTKTKAAVKILDSKGGQCHPSYSSNQSKHSTWEKGIWHLLPPGSSSSTTSGSFHLERRPLPSSMQLAVIRGDTLRGFDSTDLSQRKLRTRGNGKTVRPSSVYRNGTDIIYNYHGIVSLKLEDDSFPTHKRKAKVSIISQPQKTIKVAELPQADKVESTTDSHFPRQDQLPSFPKNCTLELKGLFHFEEGIQKLYQCNGIAWKAWSPQTKDVEDKSCPAGWHQHSGYCHILITEQKGTWNAAAQACREQYLGNLVTVFSRQHMRWLWDIGGRKSFWIGLNDQVHAGHWEWIGGEPVAFTNGRRGPSQRSKLGKSCVLVQRQGKWQTKDCRRAKPHNYVCSRKL

Target ORF information:

RefSeq Version	XM_017014320.1
Organism	Homo sapiens(human)
Definition	Homo sapiens FRAS1 related extracellular matrix 1 (FREM1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017014320.1

ORF Insert Sequence:

ATGAACTCTC TGAGTTGGGG GGCTGCGAAT GCCGTGCTGC TGCTGCTCCT CCTGGCCTGG 
GCCAGCCCCA CCTTCATCAG CATCAACCGC GGGGTGAGGG TGATGAAGGG CCACTCTGCC 
TTCCTGTCAG GAGATGACCT GAAGTTTGCC ATCCCTAAAG AGAAAGATGC CTGCAAAGTG 
GAAGTTGTGA TGAATGAGCC AATAACCCAG AGGGTTGGGA AACTCACTCC ACAGGTCTTT 
GACTGCCATT TCCTTCCCAA CGAAGTCAAG TATGTTCACA ATGGTTGTCC AATTCTTGAT 
GAAGACACAG TGAAGCTCAG ACTTTACAGA TTTACTGAAA GAGATACCTT CATAGAAACT 
TTTATCCTGT GGGTCTATCT CCTGGAACCA GACTGTAACA TCATCCATAT GAGTAACAAT 
GTGCTGGAGG TGCCTGAATT CAATGGCTTG TCCCAAGCGA TTGATAAAAA TCTGCTCAGA 
TTCGATTATG ATAGGATGGC TAGCCTGGAA TGTACCGTCA GCCTGGACAC TGCGAGAACT 
CGGCTGCCAG CCCATGGCCA GATGGTTCTC GGGGAGCCTC GACCAGAAGA ACCTCGTGGA 
GATCAGCCAC ACAGTTTTTT CCCAGAGTCC CGTATGATGT CTTTTAATGC CTTTCTGGAA 
CTGAGAGCAA AACTGAAGTG TCCAGGTGGG AGCTGTACCC CAGGATTAAA GAAAATAGGA 
AGCCTCAAAG TGAGCTGTGA GGAGTTCCTG CTGATGGGCC TTCGTTATCA GCATCTGGAT 
CCCCCTTCAC CCAACATTGA TTATATCTCC ATCCAACTGG ACCTCACAGA TACCAGGAGC 
AAAATTGTGT ACAAGTCAGA GAGTGCGTGG CTGCCTGTCT ATATCAGAGC TGGAATTCCG 
AATCAGATTC CAAAGGCTGC ATTCATGGCC GTGTTTATTC TGGAAGTGGA TCAGTTCATC 
CTGACCTCCT TGACTACATC AGTTCTGGAC TGTGAAGAAG ATGAGACCCC TAAACCCTTG 
CTGGTGTTCA ACATTACTAA AGCCCCGCTC CAGGGCTATG TGACTCACCT GTTGGATCAC 
ACCAGACCAA TCTCCTCATT CACCTGGAAA GATCTCAGTG ACATGCAGAT CGCCTATCAG 
CCACCAAACA GCAGCCATTC TGAGAGGAGA CATGATGAGG TAGAATTGGA GGTATACGAC 
TTCTTCTTTG AAAGGAGTGC ACCTATGACA GTCCACATCT CCATCAGAAC AGCAGATACA 
AATGCCCCCC GTGTATCCTG GAATACAGGT CTGAGTCTCC TTGAGGGGCA GTCTCGAGCC 
ATCACTTGGG AACAGTTTCA GGTTGTCGAC AATGACGACA TTGGTGCTGT CCGGCTAGTC 
ACCGTTGGTG GCCTGCAGCA TGGATGGCTG ACTTTAAGAG GGGGGAAAGG GTTTCTCTTC 
ACCGTGGCTG ACCTCCAGGC TGGAGTTGTT CGCTATCATC ATGATGACAG CGACTCCACC 
AAAGACTTCG TGGTCTTCCG GATATTTGAT GGCCATCACA GCATCCGTCA CAAATTCCCC 
ATCAACGTCT TGCCCAAAGA TGATAGTCCC CCGTTCCTCA TAACCAATGT TGTGATTGAA 
CTGGAGGAGG GGCAGACCAT CCTGATCCAG GGATCCATGC TGCGAGCTTC AGATGTGGAC 
GCCAGTGATG ACTACATCTT CTTCAATATC ACAAAGCCTC CACAGGCTGG GGAGATCATG 
AAGAAGCCAG GGCCAGGACT GATAGGCTAT CCTGTCCATG GCTTCCTTCA GAGGGATTTG 
TTTAATGGAA TCATTTATTA TCGTCATTTT GGTGGAGAAA TCTTTGAAGA TTCTTTTCAA 
TTTGTCCTGT GGGACAGCCA TGAACCTCCA AATCTTTCAG TGCCACAGGT GGCAACAATC 
CATATAACTC CAGTGGATGA CCAGCTTCCA AAAGAGGCTC CTGGAGTTTC TCGGCATTTG 
GTTGTCAAGG AAACTGAGGT GGCCTATATA ACTAAGAAAC AGCTACATTT TATAGATTCA 
GAATCATATG ACAGGGAGCT GGTCTACACA ATAACTACTC CTCCATTTTT CTCCTTCAGC 
CACAGACACT TGGATGCTGG GAAATTATTT ATGGTGGACA GCATACCAAA AGTAGTTAAG 
AATCCTACGG CCCTGGAGCT GAGGTCATTC ACTCAGCATG CTGTGAACTA TATGAAAGTG 
GCCTACATGC CCCCCATGCA AGACATTGGT CCCCATTGCA GAGATGTCCA GTTCACATTT 
TCTGTCAGTA ACCAACATGG CGGTACTTTG CATGGGATCT GCTTTAACAT TACAATCCTC 
CCAGTGGACA ATCAAGTTCC TGAGGCGTTC ACCAACCCTC TGAAAGTGAC TGAGGGAGGT 
CAAAGCATCA TCAGCACAGA GCACATTCTA ATTTCTGATG CAGATACCAA GCTGGACAAT 
ATTGACCTCT CCCTGCGGGA ATTGCCTCTG CACGGAAGGG TGGAGCTGAA TGGATTTCCT 
CTAAATTCAG GGGGCACATT TTCTTGGGGC GATCTCCATA CCTTAAAAGT TAGGTATCAA 
CATGATGGAA CTGAAGTTCT TCAGGATGAC CTACTCTTGG AGGTCACCGA TGGCACAAAT 
TCAGCAGAAT TTGTACTACA TGTTGAGGTA TTCCCTGTCA ACGATGAGCC ACCAGTCTTA 
AAGGCTGACC TCATGCCTGT CATGAATTGC TCAGAGGGAG GAGAGGTGGT CATCACCTCT 
GAATACATTT TTGCTACTGA TGTGGACAGC GATAACTTGA AGTTGATGTT TGTGATTGCT 
CGCGAACCTC AGCATGGGGT GGTGAGGAGA GCTGGAGTCA CAGTGGATCA GTTCTCTCAG 
AGAGATGTTA TCTCAGAGGC CGTGACATAC AAACACACAG GTGGCGAGAT TGGCCTGATG 
CCTTGTTTTG ACACCATTAC ATTGGTGGTT TCGGATGGAG AGGCTGGCCC TTTTGTGAAT 
GGCTGTTGCT ACAATGGACC CAATCCATCT GTTCCACTTC ATGCGTCCTT TCCAGTGTAT 
GATCTCAACA TCACGGTATA CCCAGTAGAC AACCAGCCAC CTTCCATTGC AATAGGTCCC 
GTGTTTGTTG TAGATGAGGG CTGCTCAACA GCCCTTACTG TTAACCATTT GTCCGCCACT 
GACCCTGACA CTGCAGCAGA TGACTTGGAA TTTGTTTTGG TTTCTCCTCC TCAGTTTGGC 
TACCTCGAAA ATATACTCCC TTCTGTGGGT TTTGAAAAAA GCAATATTGG CATAAGTATA 
GATTCATTTC AGTGGAAAGA CATGAACGCT TTTCACATTA ACTATGTGCA GTCCAGGCAT 
CTGAGGATAG AACCAACTGC CGACCAGTTC ACGGTGTACG TCACAGATGG GAAGCATCAC 
TCCTTGGAGA TACCATTTTC TATTATAATC AACCCCACAA ATGATGAAGC TCCTGACTTT 
GTAGTGCAGA ATATTACTGT GTGTGAGGGT CAGATGAAAG AGCTGGACTC TTCCATCATC 
AGCGCTGTGG ACCTGGACAT TCCCCAGGAT GCCCTGCTGT TCAGCATCAC TCAAAAGCCA 
CGCCATGGCC TCCTCATCGA TAGGGGGTTT AGCAAAGACT TCTCTGAGAA TAAGCAGCCA 
GCCAACCCTC ACCAGAAACA TGCACCTGTT CACAGCTTTT CCATGGAACT CCTCAAGACT 
GGAATGAGGT TGACGTACAT GCATGATGAC TCAGAGAGCC TTGCTGATGA TTTTACAATC 
CAATTGTCAG ATGGGAAACA TAAGATACTT AAAACCATTT CAGTAGAGGT CATCCCAGTT 
AATGATGAAA AACCAATGCT GAGCAAGAAG GCTGAAATTG CAATGAATAT GGGTGAAACT 
CGTATTATTT CCAGTGCTAT TCTTTCAGCC ATAGATGAAG ACTCACCCAG GGAGAAGATT 
TACTATGTAT TTGAAAGGCT TCCCCAAAAT GGGCAACTTC AGCTTAAGAT AGGGAGGGAC 
TGGGTTCCTC TCTCCCCTGG CATGAAATGC ACTCAGGAGG AAGTGGATCT GAACTTGCTG 
AGATACACAC ACACCGGGGC AATGGATTCC CAGAATCAAG ATAGCTTCAC CTTCTACCTT 
TGGGATGGCA ACAACAGGTC CCCTGCTCTT GACTGTCAAA TCACCATCAA GGACATGGAA 
AAAGGTGATA TTGTCATCCT TACAAAACCA CTCGTGGTGT CTAAAGGTGA TAGAGGTTTC 
TTAACAACCA CCACGCTCCT GGCTGTGGAC GGAACAGACA AGCCTGAGGA ACTGCTCTAT 
GTCATCACCT CCCCTCCGCG ATATGGCCAG ATCGAATATG TTCACTATCC TGGAGTTCCC 
ATTACAAACT TCAGCCAAAT GGATGTAGTG GGGCAGACAG TTTGCTATGT ACACAAGAGC 
AAGGTGACTG TCTCCAGTGA CAGATTCAGA TTCATCATCA GCAATGGACT GCGGACCGAG 
CACGGGGTGT TTGAGATCAC ACTGGAGACT GTGGACAGAG CCCTGCCTGT GGTAACCAGG 
AACAAGGGGT TGAGACTGGC CCAAGGGGCC GTGGGCCTGC TTTCCCCTGA CCTCCTTCAG 
CTGACCGACC CTGATACACC TGCGGAGAAC CTCACCTTCC TCTTGGTTCA GCTCCCCCAG 
CATGGCCAGC TCTACCTGTG GGGGACAGGG CTACTTCAAC ACAATTTCAC CCAGCAGGAT 
GTGGACAGCA AGAATGTGGC CTATCGGCAC TCAGGAGGGG ACTCCCAGAC TGACTGCTTT 
ACTTTCATGG CCACAGATGG GACAAACCAA GGCTTTATTG TGAATGGGAG AGTGTGGGAA 
GAACCTGTTT TATTCACCAT TCAGGTAGAC CAATTGGACA AAACAGCTCC TCGTATCACA 
CTCTTGCATT CCCCTTCTCA AGTGGGGCTC CTGAAAAATG GCTGCTACGG GATTTACATC 
ACTTCCCGCG TGTTGAAGGC ATCAGACCCT GACACTGAGG ACGATCAGAT CATCTTTAAA 
ATTCTACAAG GCCCAAAACA TGGACATCTG GAGAACACAA CAACAGGTGA ATTTATCCAT 
GAGAAATTTA GCCAAAAGGA CTTAAACAGT AAGACTATTC TTTACATCAT AAACCCATCT 
TTGGAAGTAA ATTCAGATAC CGTGGAATTT CAAATCATGG ACCCCACAGG GAACTCGGCC 
ACTCCTCAAA TTTTGGAACT GAAGTGGTCT CATATTGAAT GGTCACAGAC CGAATATGAA 
GTCTGTGAGA ATGTGGGTTT GTTGCCCTTG GAAATTATCA GAAGGGGATA TTCCATGGAC 
TCGGCCTTTG TGGGTATAAA GGTCAACCAA GTGTCAGCTG CAGTTGGAAA AGATTTCACC 
GTGATTCCAT CTAAACTGAT TCAGTTTGAC CCAGGAATGT CAACTAAGAT GTGGAATATA 
GCAATTACCT ATGACGGATT AGAGGAAGAT GATGAGGTCT TTGAAGTAAT TCTGAACTCC 
CCTGTGAATG CAGTTCTTGG CACAAAGACA AAAGCTGCAG TGAAAATTTT GGACTCAAAA 
GGAGGACAAT GCCATCCTTC ATATTCCTCC AACCAAAGCA AGCACAGCAC ATGGGAGAAG 
GGCATTTGGC ATCTGCTGCC CCCAGGGTCT TCCTCATCCA CCACTTCTGG TTCCTTTCAT 
CTGGAAAGAA GACCTCTTCC ATCTTCCATG CAGCTAGCAG TCATCAGGGG AGACACCCTG 
CGGGGCTTTG ATTCTACAGA TCTTTCTCAA AGGAAGCTTA GGACCCGTGG GAATGGCAAA 
ACAGTTCGTC CATCCTCTGT TTATAGAAAT GGAACAGACA TCATCTATAA TTATCATGGG 
ATAGTTTCCT TGAAACTGGA GGATGACAGT TTCCCAACTC ACAAAAGGAA GGCCAAAGTA 
TCCATCATTA GTCAGCCACA AAAGACAATC AAAGTGGCAG AACTGCCTCA AGCAGATAAG 
GTGGAATCCA CAACTGACTC ACACTTCCCC AGACAGGACC AGTTGCCCTC ATTTCCAAAG 
AACTGCACTC TGGAATTAAA GGGACTCTTC CATTTTGAAG AAGGCATCCA GAAGCTGTAT 
CAGTGCAATG GGATCGCCTG GAAAGCCTGG AGTCCCCAAA CCAAGGATGT GGAAGACAAA 
TCCTGTCCAG CCGGGTGGCA CCAGCACTCA GGCTACTGTC ACATCTTGAT CACAGAGCAG 
AAAGGCACCT GGAATGCGGC TGCCCAAGCT TGCAGGGAAC AATACCTGGG CAACCTTGTA 
ACTGTATTCT CCAGGCAGCA CATGCGGTGG CTCTGGGACA TTGGTGGGAG AAAGTCCTTT 
TGGATAGGTT TGAACGACCA AGTGCATGCT GGCCACTGGG AGTGGATCGG TGGTGAACCT 
GTTGCCTTCA CCAATGGGAG AAGAGGGCCC TCTCAACGCT CCAAGCTTGG AAAGAGCTGT 
GTTTTGGTTC AAAGACAAGG GAAATGGCAA ACAAAAGACT GTAGGAGAGC CAAACCTCAT 
AATTATGTGT GTTCCAGAAA ACTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FREM1 ( XM_017014318.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016869807.1 Homo sapiens FRAS1 related extracellular matrix 1 (FREM1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHu98459
Clone ID Related Accession (Same CDS sequence)	XM_017014320.1 , XM_017014318.1 , XM_017014316.1 , XM_017014316.2 , XM_017014317.1 , XM_017014319.2 , XM_017014321.2 , XM_017014323.1 , XM_017014322.1 , XM_017014321.1 , XM_017014320.2 , XM_017014319.1
Accession Version	XM_017014318.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6567bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	FRAS1-related extracellular matrix protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACTCTC TGAGTTGGGG GGCTGCGAAT GCCGTGCTGC TGCTGCTCCT CCTGGCCTGG 
GCCAGCCCCA CCTTCATCAG CATCAACCGC GGGGTGAGGG TGATGAAGGG CCACTCTGCC 
TTCCTGTCAG GAGATGACCT GAAGTTTGCC ATCCCTAAAG AGAAAGATGC CTGCAAAGTG 
GAAGTTGTGA TGAATGAGCC AATAACCCAG AGGGTTGGGA AACTCACTCC ACAGGTCTTT 
GACTGCCATT TCCTTCCCAA CGAAGTCAAG TATGTTCACA ATGGTTGTCC AATTCTTGAT 
GAAGACACAG TGAAGCTCAG ACTTTACAGA TTTACTGAAA GAGATACCTT CATAGAAACT 
TTTATCCTGT GGGTCTATCT CCTGGAACCA GACTGTAACA TCATCCATAT GAGTAACAAT 
GTGCTGGAGG TGCCTGAATT CAATGGCTTG TCCCAAGCGA TTGATAAAAA TCTGCTCAGA 
TTCGATTATG ATAGGATGGC TAGCCTGGAA TGTACCGTCA GCCTGGACAC TGCGAGAACT 
CGGCTGCCAG CCCATGGCCA GATGGTTCTC GGGGAGCCTC GACCAGAAGA ACCTCGTGGA 
GATCAGCCAC ACAGTTTTTT CCCAGAGTCC CGTATGATGT CTTTTAATGC CTTTCTGGAA 
CTGAGAGCAA AACTGAAGTG TCCAGGTGGG AGCTGTACCC CAGGATTAAA GAAAATAGGA 
AGCCTCAAAG TGAGCTGTGA GGAGTTCCTG CTGATGGGCC TTCGTTATCA GCATCTGGAT 
CCCCCTTCAC CCAACATTGA TTATATCTCC ATCCAACTGG ACCTCACAGA TACCAGGAGC 
AAAATTGTGT ACAAGTCAGA GAGTGCGTGG CTGCCTGTCT ATATCAGAGC TGGAATTCCG 
AATCAGATTC CAAAGGCTGC ATTCATGGCC GTGTTTATTC TGGAAGTGGA TCAGTTCATC 
CTGACCTCCT TGACTACATC AGTTCTGGAC TGTGAAGAAG ATGAGACCCC TAAACCCTTG 
CTGGTGTTCA ACATTACTAA AGCCCCGCTC CAGGGCTATG TGACTCACCT GTTGGATCAC 
ACCAGACCAA TCTCCTCATT CACCTGGAAA GATCTCAGTG ACATGCAGAT CGCCTATCAG 
CCACCAAACA GCAGCCATTC TGAGAGGAGA CATGATGAGG TAGAATTGGA GGTATACGAC 
TTCTTCTTTG AAAGGAGTGC ACCTATGACA GTCCACATCT CCATCAGAAC AGCAGATACA 
AATGCCCCCC GTGTATCCTG GAATACAGGT CTGAGTCTCC TTGAGGGGCA GTCTCGAGCC 
ATCACTTGGG AACAGTTTCA GGTTGTCGAC AATGACGACA TTGGTGCTGT CCGGCTAGTC 
ACCGTTGGTG GCCTGCAGCA TGGATGGCTG ACTTTAAGAG GGGGGAAAGG GTTTCTCTTC 
ACCGTGGCTG ACCTCCAGGC TGGAGTTGTT CGCTATCATC ATGATGACAG CGACTCCACC 
AAAGACTTCG TGGTCTTCCG GATATTTGAT GGCCATCACA GCATCCGTCA CAAATTCCCC 
ATCAACGTCT TGCCCAAAGA TGATAGTCCC CCGTTCCTCA TAACCAATGT TGTGATTGAA 
CTGGAGGAGG GGCAGACCAT CCTGATCCAG GGATCCATGC TGCGAGCTTC AGATGTGGAC 
GCCAGTGATG ACTACATCTT CTTCAATATC ACAAAGCCTC CACAGGCTGG GGAGATCATG 
AAGAAGCCAG GGCCAGGACT GATAGGCTAT CCTGTCCATG GCTTCCTTCA GAGGGATTTG 
TTTAATGGAA TCATTTATTA TCGTCATTTT GGTGGAGAAA TCTTTGAAGA TTCTTTTCAA 
TTTGTCCTGT GGGACAGCCA TGAACCTCCA AATCTTTCAG TGCCACAGGT GGCAACAATC 
CATATAACTC CAGTGGATGA CCAGCTTCCA AAAGAGGCTC CTGGAGTTTC TCGGCATTTG 
GTTGTCAAGG AAACTGAGGT GGCCTATATA ACTAAGAAAC AGCTACATTT TATAGATTCA 
GAATCATATG ACAGGGAGCT GGTCTACACA ATAACTACTC CTCCATTTTT CTCCTTCAGC 
CACAGACACT TGGATGCTGG GAAATTATTT ATGGTGGACA GCATACCAAA AGTAGTTAAG 
AATCCTACGG CCCTGGAGCT GAGGTCATTC ACTCAGCATG CTGTGAACTA TATGAAAGTG 
GCCTACATGC CCCCCATGCA AGACATTGGT CCCCATTGCA GAGATGTCCA GTTCACATTT 
TCTGTCAGTA ACCAACATGG CGGTACTTTG CATGGGATCT GCTTTAACAT TACAATCCTC 
CCAGTGGACA ATCAAGTTCC TGAGGCGTTC ACCAACCCTC TGAAAGTGAC TGAGGGAGGT 
CAAAGCATCA TCAGCACAGA GCACATTCTA ATTTCTGATG CAGATACCAA GCTGGACAAT 
ATTGACCTCT CCCTGCGGGA ATTGCCTCTG CACGGAAGGG TGGAGCTGAA TGGATTTCCT 
CTAAATTCAG GGGGCACATT TTCTTGGGGC GATCTCCATA CCTTAAAAGT TAGGTATCAA 
CATGATGGAA CTGAAGTTCT TCAGGATGAC CTACTCTTGG AGGTCACCGA TGGCACAAAT 
TCAGCAGAAT TTGTACTACA TGTTGAGGTA TTCCCTGTCA ACGATGAGCC ACCAGTCTTA 
AAGGCTGACC TCATGCCTGT CATGAATTGC TCAGAGGGAG GAGAGGTGGT CATCACCTCT 
GAATACATTT TTGCTACTGA TGTGGACAGC GATAACTTGA AGTTGATGTT TGTGATTGCT 
CGCGAACCTC AGCATGGGGT GGTGAGGAGA GCTGGAGTCA CAGTGGATCA GTTCTCTCAG 
AGAGATGTTA TCTCAGAGGC CGTGACATAC AAACACACAG GTGGCGAGAT TGGCCTGATG 
CCTTGTTTTG ACACCATTAC ATTGGTGGTT TCGGATGGAG AGGCTGGCCC TTTTGTGAAT 
GGCTGTTGCT ACAATGGACC CAATCCATCT GTTCCACTTC ATGCGTCCTT TCCAGTGTAT 
GATCTCAACA TCACGGTATA CCCAGTAGAC AACCAGCCAC CTTCCATTGC AATAGGTCCC 
GTGTTTGTTG TAGATGAGGG CTGCTCAACA GCCCTTACTG TTAACCATTT GTCCGCCACT 
GACCCTGACA CTGCAGCAGA TGACTTGGAA TTTGTTTTGG TTTCTCCTCC TCAGTTTGGC 
TACCTCGAAA ATATACTCCC TTCTGTGGGT TTTGAAAAAA GCAATATTGG CATAAGTATA 
GATTCATTTC AGTGGAAAGA CATGAACGCT TTTCACATTA ACTATGTGCA GTCCAGGCAT 
CTGAGGATAG AACCAACTGC CGACCAGTTC ACGGTGTACG TCACAGATGG GAAGCATCAC 
TCCTTGGAGA TACCATTTTC TATTATAATC AACCCCACAA ATGATGAAGC TCCTGACTTT 
GTAGTGCAGA ATATTACTGT GTGTGAGGGT CAGATGAAAG AGCTGGACTC TTCCATCATC 
AGCGCTGTGG ACCTGGACAT TCCCCAGGAT GCCCTGCTGT TCAGCATCAC TCAAAAGCCA 
CGCCATGGCC TCCTCATCGA TAGGGGGTTT AGCAAAGACT TCTCTGAGAA TAAGCAGCCA 
GCCAACCCTC ACCAGAAACA TGCACCTGTT CACAGCTTTT CCATGGAACT CCTCAAGACT 
GGAATGAGGT TGACGTACAT GCATGATGAC TCAGAGAGCC TTGCTGATGA TTTTACAATC 
CAATTGTCAG ATGGGAAACA TAAGATACTT AAAACCATTT CAGTAGAGGT CATCCCAGTT 
AATGATGAAA AACCAATGCT GAGCAAGAAG GCTGAAATTG CAATGAATAT GGGTGAAACT 
CGTATTATTT CCAGTGCTAT TCTTTCAGCC ATAGATGAAG ACTCACCCAG GGAGAAGATT 
TACTATGTAT TTGAAAGGCT TCCCCAAAAT GGGCAACTTC AGCTTAAGAT AGGGAGGGAC 
TGGGTTCCTC TCTCCCCTGG CATGAAATGC ACTCAGGAGG AAGTGGATCT GAACTTGCTG 
AGATACACAC ACACCGGGGC AATGGATTCC CAGAATCAAG ATAGCTTCAC CTTCTACCTT 
TGGGATGGCA ACAACAGGTC CCCTGCTCTT GACTGTCAAA TCACCATCAA GGACATGGAA 
AAAGGTGATA TTGTCATCCT TACAAAACCA CTCGTGGTGT CTAAAGGTGA TAGAGGTTTC 
TTAACAACCA CCACGCTCCT GGCTGTGGAC GGAACAGACA AGCCTGAGGA ACTGCTCTAT 
GTCATCACCT CCCCTCCGCG ATATGGCCAG ATCGAATATG TTCACTATCC TGGAGTTCCC 
ATTACAAACT TCAGCCAAAT GGATGTAGTG GGGCAGACAG TTTGCTATGT ACACAAGAGC 
AAGGTGACTG TCTCCAGTGA CAGATTCAGA TTCATCATCA GCAATGGACT GCGGACCGAG 
CACGGGGTGT TTGAGATCAC ACTGGAGACT GTGGACAGAG CCCTGCCTGT GGTAACCAGG 
AACAAGGGGT TGAGACTGGC CCAAGGGGCC GTGGGCCTGC TTTCCCCTGA CCTCCTTCAG 
CTGACCGACC CTGATACACC TGCGGAGAAC CTCACCTTCC TCTTGGTTCA GCTCCCCCAG 
CATGGCCAGC TCTACCTGTG GGGGACAGGG CTACTTCAAC ACAATTTCAC CCAGCAGGAT 
GTGGACAGCA AGAATGTGGC CTATCGGCAC TCAGGAGGGG ACTCCCAGAC TGACTGCTTT 
ACTTTCATGG CCACAGATGG GACAAACCAA GGCTTTATTG TGAATGGGAG AGTGTGGGAA 
GAACCTGTTT TATTCACCAT TCAGGTAGAC CAATTGGACA AAACAGCTCC TCGTATCACA 
CTCTTGCATT CCCCTTCTCA AGTGGGGCTC CTGAAAAATG GCTGCTACGG GATTTACATC 
ACTTCCCGCG TGTTGAAGGC ATCAGACCCT GACACTGAGG ACGATCAGAT CATCTTTAAA 
ATTCTACAAG GCCCAAAACA TGGACATCTG GAGAACACAA CAACAGGTGA ATTTATCCAT 
GAGAAATTTA GCCAAAAGGA CTTAAACAGT AAGACTATTC TTTACATCAT AAACCCATCT 
TTGGAAGTAA ATTCAGATAC CGTGGAATTT CAAATCATGG ACCCCACAGG GAACTCGGCC 
ACTCCTCAAA TTTTGGAACT GAAGTGGTCT CATATTGAAT GGTCACAGAC CGAATATGAA 
GTCTGTGAGA ATGTGGGTTT GTTGCCCTTG GAAATTATCA GAAGGGGATA TTCCATGGAC 
TCGGCCTTTG TGGGTATAAA GGTCAACCAA GTGTCAGCTG CAGTTGGAAA AGATTTCACC 
GTGATTCCAT CTAAACTGAT TCAGTTTGAC CCAGGAATGT CAACTAAGAT GTGGAATATA 
GCAATTACCT ATGACGGATT AGAGGAAGAT GATGAGGTCT TTGAAGTAAT TCTGAACTCC 
CCTGTGAATG CAGTTCTTGG CACAAAGACA AAAGCTGCAG TGAAAATTTT GGACTCAAAA 
GGAGGACAAT GCCATCCTTC ATATTCCTCC AACCAAAGCA AGCACAGCAC ATGGGAGAAG 
GGCATTTGGC ATCTGCTGCC CCCAGGGTCT TCCTCATCCA CCACTTCTGG TTCCTTTCAT 
CTGGAAAGAA GACCTCTTCC ATCTTCCATG CAGCTAGCAG TCATCAGGGG AGACACCCTG 
CGGGGCTTTG ATTCTACAGA TCTTTCTCAA AGGAAGCTTA GGACCCGTGG GAATGGCAAA 
ACAGTTCGTC CATCCTCTGT TTATAGAAAT GGAACAGACA TCATCTATAA TTATCATGGG 
ATAGTTTCCT TGAAACTGGA GGATGACAGT TTCCCAACTC ACAAAAGGAA GGCCAAAGTA 
TCCATCATTA GTCAGCCACA AAAGACAATC AAAGTGGCAG AACTGCCTCA AGCAGATAAG 
GTGGAATCCA CAACTGACTC ACACTTCCCC AGACAGGACC AGTTGCCCTC ATTTCCAAAG 
AACTGCACTC TGGAATTAAA GGGACTCTTC CATTTTGAAG AAGGCATCCA GAAGCTGTAT 
CAGTGCAATG GGATCGCCTG GAAAGCCTGG AGTCCCCAAA CCAAGGATGT GGAAGACAAA 
TCCTGTCCAG CCGGGTGGCA CCAGCACTCA GGCTACTGTC ACATCTTGAT CACAGAGCAG 
AAAGGCACCT GGAATGCGGC TGCCCAAGCT TGCAGGGAAC AATACCTGGG CAACCTTGTA 
ACTGTATTCT CCAGGCAGCA CATGCGGTGG CTCTGGGACA TTGGTGGGAG AAAGTCCTTT 
TGGATAGGTT TGAACGACCA AGTGCATGCT GGCCACTGGG AGTGGATCGG TGGTGAACCT 
GTTGCCTTCA CCAATGGGAG AAGAGGGCCC TCTCAACGCT CCAAGCTTGG AAAGAGCTGT 
GTTTTGGTTC AAAGACAAGG GAAATGGCAA ACAAAAGACT GTAGGAGAGC CAAACCTCAT 
AATTATGTGT GTTCCAGAAA ACTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016869807.1
CDS	1363..7929
Translation	MNSLSWGAANAVLLLLLLAWASPTFISINRGVRVMKGHSAFLSGDDLKFAIPKEKDACKVEVVMNEPITQRVGKLTPQVFDCHFLPNEVKYVHNGCPILDEDTVKLRLYRFTERDTFIETFILWVYLLEPDCNIIHMSNNVLEVPEFNGLSQAIDKNLLRFDYDRMASLECTVSLDTARTRLPAHGQMVLGEPRPEEPRGDQPHSFFPESRMMSFNAFLELRAKLKCPGGSCTPGLKKIGSLKVSCEEFLLMGLRYQHLDPPSPNIDYISIQLDLTDTRSKIVYKSESAWLPVYIRAGIPNQIPKAAFMAVFILEVDQFILTSLTTSVLDCEEDETPKPLLVFNITKAPLQGYVTHLLDHTRPISSFTWKDLSDMQIAYQPPNSSHSERRHDEVELEVYDFFFERSAPMTVHISIRTADTNAPRVSWNTGLSLLEGQSRAITWEQFQVVDNDDIGAVRLVTVGGLQHGWLTLRGGKGFLFTVADLQAGVVRYHHDDSDSTKDFVVFRIFDGHHSIRHKFPINVLPKDDSPPFLITNVVIELEEGQTILIQGSMLRASDVDASDDYIFFNITKPPQAGEIMKKPGPGLIGYPVHGFLQRDLFNGIIYYRHFGGEIFEDSFQFVLWDSHEPPNLSVPQVATIHITPVDDQLPKEAPGVSRHLVVKETEVAYITKKQLHFIDSESYDRELVYTITTPPFFSFSHRHLDAGKLFMVDSIPKVVKNPTALELRSFTQHAVNYMKVAYMPPMQDIGPHCRDVQFTFSVSNQHGGTLHGICFNITILPVDNQVPEAFTNPLKVTEGGQSIISTEHILISDADTKLDNIDLSLRELPLHGRVELNGFPLNSGGTFSWGDLHTLKVRYQHDGTEVLQDDLLLEVTDGTNSAEFVLHVEVFPVNDEPPVLKADLMPVMNCSEGGEVVITSEYIFATDVDSDNLKLMFVIAREPQHGVVRRAGVTVDQFSQRDVISEAVTYKHTGGEIGLMPCFDTITLVVSDGEAGPFVNGCCYNGPNPSVPLHASFPVYDLNITVYPVDNQPPSIAIGPVFVVDEGCSTALTVNHLSATDPDTAADDLEFVLVSPPQFGYLENILPSVGFEKSNIGISIDSFQWKDMNAFHINYVQSRHLRIEPTADQFTVYVTDGKHHSLEIPFSIIINPTNDEAPDFVVQNITVCEGQMKELDSSIISAVDLDIPQDALLFSITQKPRHGLLIDRGFSKDFSENKQPANPHQKHAPVHSFSMELLKTGMRLTYMHDDSESLADDFTIQLSDGKHKILKTISVEVIPVNDEKPMLSKKAEIAMNMGETRIISSAILSAIDEDSPREKIYYVFERLPQNGQLQLKIGRDWVPLSPGMKCTQEEVDLNLLRYTHTGAMDSQNQDSFTFYLWDGNNRSPALDCQITIKDMEKGDIVILTKPLVVSKGDRGFLTTTTLLAVDGTDKPEELLYVITSPPRYGQIEYVHYPGVPITNFSQMDVVGQTVCYVHKSKVTVSSDRFRFIISNGLRTEHGVFEITLETVDRALPVVTRNKGLRLAQGAVGLLSPDLLQLTDPDTPAENLTFLLVQLPQHGQLYLWGTGLLQHNFTQQDVDSKNVAYRHSGGDSQTDCFTFMATDGTNQGFIVNGRVWEEPVLFTIQVDQLDKTAPRITLLHSPSQVGLLKNGCYGIYITSRVLKASDPDTEDDQIIFKILQGPKHGHLENTTTGEFIHEKFSQKDLNSKTILYIINPSLEVNSDTVEFQIMDPTGNSATPQILELKWSHIEWSQTEYEVCENVGLLPLEIIRRGYSMDSAFVGIKVNQVSAAVGKDFTVIPSKLIQFDPGMSTKMWNIAITYDGLEEDDEVFEVILNSPVNAVLGTKTKAAVKILDSKGGQCHPSYSSNQSKHSTWEKGIWHLLPPGSSSSTTSGSFHLERRPLPSSMQLAVIRGDTLRGFDSTDLSQRKLRTRGNGKTVRPSSVYRNGTDIIYNYHGIVSLKLEDDSFPTHKRKAKVSIISQPQKTIKVAELPQADKVESTTDSHFPRQDQLPSFPKNCTLELKGLFHFEEGIQKLYQCNGIAWKAWSPQTKDVEDKSCPAGWHQHSGYCHILITEQKGTWNAAAQACREQYLGNLVTVFSRQHMRWLWDIGGRKSFWIGLNDQVHAGHWEWIGGEPVAFTNGRRGPSQRSKLGKSCVLVQRQGKWQTKDCRRAKPHNYVCSRKL

Target ORF information:

RefSeq Version	XM_017014318.1
Organism	Homo sapiens(human)
Definition	Homo sapiens FRAS1 related extracellular matrix 1 (FREM1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017014318.1

ORF Insert Sequence:

ATGAACTCTC TGAGTTGGGG GGCTGCGAAT GCCGTGCTGC TGCTGCTCCT CCTGGCCTGG 
GCCAGCCCCA CCTTCATCAG CATCAACCGC GGGGTGAGGG TGATGAAGGG CCACTCTGCC 
TTCCTGTCAG GAGATGACCT GAAGTTTGCC ATCCCTAAAG AGAAAGATGC CTGCAAAGTG 
GAAGTTGTGA TGAATGAGCC AATAACCCAG AGGGTTGGGA AACTCACTCC ACAGGTCTTT 
GACTGCCATT TCCTTCCCAA CGAAGTCAAG TATGTTCACA ATGGTTGTCC AATTCTTGAT 
GAAGACACAG TGAAGCTCAG ACTTTACAGA TTTACTGAAA GAGATACCTT CATAGAAACT 
TTTATCCTGT GGGTCTATCT CCTGGAACCA GACTGTAACA TCATCCATAT GAGTAACAAT 
GTGCTGGAGG TGCCTGAATT CAATGGCTTG TCCCAAGCGA TTGATAAAAA TCTGCTCAGA 
TTCGATTATG ATAGGATGGC TAGCCTGGAA TGTACCGTCA GCCTGGACAC TGCGAGAACT 
CGGCTGCCAG CCCATGGCCA GATGGTTCTC GGGGAGCCTC GACCAGAAGA ACCTCGTGGA 
GATCAGCCAC ACAGTTTTTT CCCAGAGTCC CGTATGATGT CTTTTAATGC CTTTCTGGAA 
CTGAGAGCAA AACTGAAGTG TCCAGGTGGG AGCTGTACCC CAGGATTAAA GAAAATAGGA 
AGCCTCAAAG TGAGCTGTGA GGAGTTCCTG CTGATGGGCC TTCGTTATCA GCATCTGGAT 
CCCCCTTCAC CCAACATTGA TTATATCTCC ATCCAACTGG ACCTCACAGA TACCAGGAGC 
AAAATTGTGT ACAAGTCAGA GAGTGCGTGG CTGCCTGTCT ATATCAGAGC TGGAATTCCG 
AATCAGATTC CAAAGGCTGC ATTCATGGCC GTGTTTATTC TGGAAGTGGA TCAGTTCATC 
CTGACCTCCT TGACTACATC AGTTCTGGAC TGTGAAGAAG ATGAGACCCC TAAACCCTTG 
CTGGTGTTCA ACATTACTAA AGCCCCGCTC CAGGGCTATG TGACTCACCT GTTGGATCAC 
ACCAGACCAA TCTCCTCATT CACCTGGAAA GATCTCAGTG ACATGCAGAT CGCCTATCAG 
CCACCAAACA GCAGCCATTC TGAGAGGAGA CATGATGAGG TAGAATTGGA GGTATACGAC 
TTCTTCTTTG AAAGGAGTGC ACCTATGACA GTCCACATCT CCATCAGAAC AGCAGATACA 
AATGCCCCCC GTGTATCCTG GAATACAGGT CTGAGTCTCC TTGAGGGGCA GTCTCGAGCC 
ATCACTTGGG AACAGTTTCA GGTTGTCGAC AATGACGACA TTGGTGCTGT CCGGCTAGTC 
ACCGTTGGTG GCCTGCAGCA TGGATGGCTG ACTTTAAGAG GGGGGAAAGG GTTTCTCTTC 
ACCGTGGCTG ACCTCCAGGC TGGAGTTGTT CGCTATCATC ATGATGACAG CGACTCCACC 
AAAGACTTCG TGGTCTTCCG GATATTTGAT GGCCATCACA GCATCCGTCA CAAATTCCCC 
ATCAACGTCT TGCCCAAAGA TGATAGTCCC CCGTTCCTCA TAACCAATGT TGTGATTGAA 
CTGGAGGAGG GGCAGACCAT CCTGATCCAG GGATCCATGC TGCGAGCTTC AGATGTGGAC 
GCCAGTGATG ACTACATCTT CTTCAATATC ACAAAGCCTC CACAGGCTGG GGAGATCATG 
AAGAAGCCAG GGCCAGGACT GATAGGCTAT CCTGTCCATG GCTTCCTTCA GAGGGATTTG 
TTTAATGGAA TCATTTATTA TCGTCATTTT GGTGGAGAAA TCTTTGAAGA TTCTTTTCAA 
TTTGTCCTGT GGGACAGCCA TGAACCTCCA AATCTTTCAG TGCCACAGGT GGCAACAATC 
CATATAACTC CAGTGGATGA CCAGCTTCCA AAAGAGGCTC CTGGAGTTTC TCGGCATTTG 
GTTGTCAAGG AAACTGAGGT GGCCTATATA ACTAAGAAAC AGCTACATTT TATAGATTCA 
GAATCATATG ACAGGGAGCT GGTCTACACA ATAACTACTC CTCCATTTTT CTCCTTCAGC 
CACAGACACT TGGATGCTGG GAAATTATTT ATGGTGGACA GCATACCAAA AGTAGTTAAG 
AATCCTACGG CCCTGGAGCT GAGGTCATTC ACTCAGCATG CTGTGAACTA TATGAAAGTG 
GCCTACATGC CCCCCATGCA AGACATTGGT CCCCATTGCA GAGATGTCCA GTTCACATTT 
TCTGTCAGTA ACCAACATGG CGGTACTTTG CATGGGATCT GCTTTAACAT TACAATCCTC 
CCAGTGGACA ATCAAGTTCC TGAGGCGTTC ACCAACCCTC TGAAAGTGAC TGAGGGAGGT 
CAAAGCATCA TCAGCACAGA GCACATTCTA ATTTCTGATG CAGATACCAA GCTGGACAAT 
ATTGACCTCT CCCTGCGGGA ATTGCCTCTG CACGGAAGGG TGGAGCTGAA TGGATTTCCT 
CTAAATTCAG GGGGCACATT TTCTTGGGGC GATCTCCATA CCTTAAAAGT TAGGTATCAA 
CATGATGGAA CTGAAGTTCT TCAGGATGAC CTACTCTTGG AGGTCACCGA TGGCACAAAT 
TCAGCAGAAT TTGTACTACA TGTTGAGGTA TTCCCTGTCA ACGATGAGCC ACCAGTCTTA 
AAGGCTGACC TCATGCCTGT CATGAATTGC TCAGAGGGAG GAGAGGTGGT CATCACCTCT 
GAATACATTT TTGCTACTGA TGTGGACAGC GATAACTTGA AGTTGATGTT TGTGATTGCT 
CGCGAACCTC AGCATGGGGT GGTGAGGAGA GCTGGAGTCA CAGTGGATCA GTTCTCTCAG 
AGAGATGTTA TCTCAGAGGC CGTGACATAC AAACACACAG GTGGCGAGAT TGGCCTGATG 
CCTTGTTTTG ACACCATTAC ATTGGTGGTT TCGGATGGAG AGGCTGGCCC TTTTGTGAAT 
GGCTGTTGCT ACAATGGACC CAATCCATCT GTTCCACTTC ATGCGTCCTT TCCAGTGTAT 
GATCTCAACA TCACGGTATA CCCAGTAGAC AACCAGCCAC CTTCCATTGC AATAGGTCCC 
GTGTTTGTTG TAGATGAGGG CTGCTCAACA GCCCTTACTG TTAACCATTT GTCCGCCACT 
GACCCTGACA CTGCAGCAGA TGACTTGGAA TTTGTTTTGG TTTCTCCTCC TCAGTTTGGC 
TACCTCGAAA ATATACTCCC TTCTGTGGGT TTTGAAAAAA GCAATATTGG CATAAGTATA 
GATTCATTTC AGTGGAAAGA CATGAACGCT TTTCACATTA ACTATGTGCA GTCCAGGCAT 
CTGAGGATAG AACCAACTGC CGACCAGTTC ACGGTGTACG TCACAGATGG GAAGCATCAC 
TCCTTGGAGA TACCATTTTC TATTATAATC AACCCCACAA ATGATGAAGC TCCTGACTTT 
GTAGTGCAGA ATATTACTGT GTGTGAGGGT CAGATGAAAG AGCTGGACTC TTCCATCATC 
AGCGCTGTGG ACCTGGACAT TCCCCAGGAT GCCCTGCTGT TCAGCATCAC TCAAAAGCCA 
CGCCATGGCC TCCTCATCGA TAGGGGGTTT AGCAAAGACT TCTCTGAGAA TAAGCAGCCA 
GCCAACCCTC ACCAGAAACA TGCACCTGTT CACAGCTTTT CCATGGAACT CCTCAAGACT 
GGAATGAGGT TGACGTACAT GCATGATGAC TCAGAGAGCC TTGCTGATGA TTTTACAATC 
CAATTGTCAG ATGGGAAACA TAAGATACTT AAAACCATTT CAGTAGAGGT CATCCCAGTT 
AATGATGAAA AACCAATGCT GAGCAAGAAG GCTGAAATTG CAATGAATAT GGGTGAAACT 
CGTATTATTT CCAGTGCTAT TCTTTCAGCC ATAGATGAAG ACTCACCCAG GGAGAAGATT 
TACTATGTAT TTGAAAGGCT TCCCCAAAAT GGGCAACTTC AGCTTAAGAT AGGGAGGGAC 
TGGGTTCCTC TCTCCCCTGG CATGAAATGC ACTCAGGAGG AAGTGGATCT GAACTTGCTG 
AGATACACAC ACACCGGGGC AATGGATTCC CAGAATCAAG ATAGCTTCAC CTTCTACCTT 
TGGGATGGCA ACAACAGGTC CCCTGCTCTT GACTGTCAAA TCACCATCAA GGACATGGAA 
AAAGGTGATA TTGTCATCCT TACAAAACCA CTCGTGGTGT CTAAAGGTGA TAGAGGTTTC 
TTAACAACCA CCACGCTCCT GGCTGTGGAC GGAACAGACA AGCCTGAGGA ACTGCTCTAT 
GTCATCACCT CCCCTCCGCG ATATGGCCAG ATCGAATATG TTCACTATCC TGGAGTTCCC 
ATTACAAACT TCAGCCAAAT GGATGTAGTG GGGCAGACAG TTTGCTATGT ACACAAGAGC 
AAGGTGACTG TCTCCAGTGA CAGATTCAGA TTCATCATCA GCAATGGACT GCGGACCGAG 
CACGGGGTGT TTGAGATCAC ACTGGAGACT GTGGACAGAG CCCTGCCTGT GGTAACCAGG 
AACAAGGGGT TGAGACTGGC CCAAGGGGCC GTGGGCCTGC TTTCCCCTGA CCTCCTTCAG 
CTGACCGACC CTGATACACC TGCGGAGAAC CTCACCTTCC TCTTGGTTCA GCTCCCCCAG 
CATGGCCAGC TCTACCTGTG GGGGACAGGG CTACTTCAAC ACAATTTCAC CCAGCAGGAT 
GTGGACAGCA AGAATGTGGC CTATCGGCAC TCAGGAGGGG ACTCCCAGAC TGACTGCTTT 
ACTTTCATGG CCACAGATGG GACAAACCAA GGCTTTATTG TGAATGGGAG AGTGTGGGAA 
GAACCTGTTT TATTCACCAT TCAGGTAGAC CAATTGGACA AAACAGCTCC TCGTATCACA 
CTCTTGCATT CCCCTTCTCA AGTGGGGCTC CTGAAAAATG GCTGCTACGG GATTTACATC 
ACTTCCCGCG TGTTGAAGGC ATCAGACCCT GACACTGAGG ACGATCAGAT CATCTTTAAA 
ATTCTACAAG GCCCAAAACA TGGACATCTG GAGAACACAA CAACAGGTGA ATTTATCCAT 
GAGAAATTTA GCCAAAAGGA CTTAAACAGT AAGACTATTC TTTACATCAT AAACCCATCT 
TTGGAAGTAA ATTCAGATAC CGTGGAATTT CAAATCATGG ACCCCACAGG GAACTCGGCC 
ACTCCTCAAA TTTTGGAACT GAAGTGGTCT CATATTGAAT GGTCACAGAC CGAATATGAA 
GTCTGTGAGA ATGTGGGTTT GTTGCCCTTG GAAATTATCA GAAGGGGATA TTCCATGGAC 
TCGGCCTTTG TGGGTATAAA GGTCAACCAA GTGTCAGCTG CAGTTGGAAA AGATTTCACC 
GTGATTCCAT CTAAACTGAT TCAGTTTGAC CCAGGAATGT CAACTAAGAT GTGGAATATA 
GCAATTACCT ATGACGGATT AGAGGAAGAT GATGAGGTCT TTGAAGTAAT TCTGAACTCC 
CCTGTGAATG CAGTTCTTGG CACAAAGACA AAAGCTGCAG TGAAAATTTT GGACTCAAAA 
GGAGGACAAT GCCATCCTTC ATATTCCTCC AACCAAAGCA AGCACAGCAC ATGGGAGAAG 
GGCATTTGGC ATCTGCTGCC CCCAGGGTCT TCCTCATCCA CCACTTCTGG TTCCTTTCAT 
CTGGAAAGAA GACCTCTTCC ATCTTCCATG CAGCTAGCAG TCATCAGGGG AGACACCCTG 
CGGGGCTTTG ATTCTACAGA TCTTTCTCAA AGGAAGCTTA GGACCCGTGG GAATGGCAAA 
ACAGTTCGTC CATCCTCTGT TTATAGAAAT GGAACAGACA TCATCTATAA TTATCATGGG 
ATAGTTTCCT TGAAACTGGA GGATGACAGT TTCCCAACTC ACAAAAGGAA GGCCAAAGTA 
TCCATCATTA GTCAGCCACA AAAGACAATC AAAGTGGCAG AACTGCCTCA AGCAGATAAG 
GTGGAATCCA CAACTGACTC ACACTTCCCC AGACAGGACC AGTTGCCCTC ATTTCCAAAG 
AACTGCACTC TGGAATTAAA GGGACTCTTC CATTTTGAAG AAGGCATCCA GAAGCTGTAT 
CAGTGCAATG GGATCGCCTG GAAAGCCTGG AGTCCCCAAA CCAAGGATGT GGAAGACAAA 
TCCTGTCCAG CCGGGTGGCA CCAGCACTCA GGCTACTGTC ACATCTTGAT CACAGAGCAG 
AAAGGCACCT GGAATGCGGC TGCCCAAGCT TGCAGGGAAC AATACCTGGG CAACCTTGTA 
ACTGTATTCT CCAGGCAGCA CATGCGGTGG CTCTGGGACA TTGGTGGGAG AAAGTCCTTT 
TGGATAGGTT TGAACGACCA AGTGCATGCT GGCCACTGGG AGTGGATCGG TGGTGAACCT 
GTTGCCTTCA CCAATGGGAG AAGAGGGCCC TCTCAACGCT CCAAGCTTGG AAAGAGCTGT 
GTTTTGGTTC AAAGACAAGG GAAATGGCAA ACAAAAGACT GTAGGAGAGC CAAACCTCAT 
AATTATGTGT GTTCCAGAAA ACTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FREM1 ( XM_017014316.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016869805.1 Homo sapiens FRAS1 related extracellular matrix 1 (FREM1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu98459
Clone ID Related Accession (Same CDS sequence)	XM_017014320.1 , XM_017014318.1 , XM_017014316.1 , XM_017014316.2 , XM_017014317.1 , XM_017014319.2 , XM_017014321.2 , XM_017014323.1 , XM_017014322.1 , XM_017014321.1 , XM_017014320.2 , XM_017014319.1
Accession Version	XM_017014316.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6567bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	FRAS1-related extracellular matrix protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACTCTC TGAGTTGGGG GGCTGCGAAT GCCGTGCTGC TGCTGCTCCT CCTGGCCTGG 
GCCAGCCCCA CCTTCATCAG CATCAACCGC GGGGTGAGGG TGATGAAGGG CCACTCTGCC 
TTCCTGTCAG GAGATGACCT GAAGTTTGCC ATCCCTAAAG AGAAAGATGC CTGCAAAGTG 
GAAGTTGTGA TGAATGAGCC AATAACCCAG AGGGTTGGGA AACTCACTCC ACAGGTCTTT 
GACTGCCATT TCCTTCCCAA CGAAGTCAAG TATGTTCACA ATGGTTGTCC AATTCTTGAT 
GAAGACACAG TGAAGCTCAG ACTTTACAGA TTTACTGAAA GAGATACCTT CATAGAAACT 
TTTATCCTGT GGGTCTATCT CCTGGAACCA GACTGTAACA TCATCCATAT GAGTAACAAT 
GTGCTGGAGG TGCCTGAATT CAATGGCTTG TCCCAAGCGA TTGATAAAAA TCTGCTCAGA 
TTCGATTATG ATAGGATGGC TAGCCTGGAA TGTACCGTCA GCCTGGACAC TGCGAGAACT 
CGGCTGCCAG CCCATGGCCA GATGGTTCTC GGGGAGCCTC GACCAGAAGA ACCTCGTGGA 
GATCAGCCAC ACAGTTTTTT CCCAGAGTCC CGTATGATGT CTTTTAATGC CTTTCTGGAA 
CTGAGAGCAA AACTGAAGTG TCCAGGTGGG AGCTGTACCC CAGGATTAAA GAAAATAGGA 
AGCCTCAAAG TGAGCTGTGA GGAGTTCCTG CTGATGGGCC TTCGTTATCA GCATCTGGAT 
CCCCCTTCAC CCAACATTGA TTATATCTCC ATCCAACTGG ACCTCACAGA TACCAGGAGC 
AAAATTGTGT ACAAGTCAGA GAGTGCGTGG CTGCCTGTCT ATATCAGAGC TGGAATTCCG 
AATCAGATTC CAAAGGCTGC ATTCATGGCC GTGTTTATTC TGGAAGTGGA TCAGTTCATC 
CTGACCTCCT TGACTACATC AGTTCTGGAC TGTGAAGAAG ATGAGACCCC TAAACCCTTG 
CTGGTGTTCA ACATTACTAA AGCCCCGCTC CAGGGCTATG TGACTCACCT GTTGGATCAC 
ACCAGACCAA TCTCCTCATT CACCTGGAAA GATCTCAGTG ACATGCAGAT CGCCTATCAG 
CCACCAAACA GCAGCCATTC TGAGAGGAGA CATGATGAGG TAGAATTGGA GGTATACGAC 
TTCTTCTTTG AAAGGAGTGC ACCTATGACA GTCCACATCT CCATCAGAAC AGCAGATACA 
AATGCCCCCC GTGTATCCTG GAATACAGGT CTGAGTCTCC TTGAGGGGCA GTCTCGAGCC 
ATCACTTGGG AACAGTTTCA GGTTGTCGAC AATGACGACA TTGGTGCTGT CCGGCTAGTC 
ACCGTTGGTG GCCTGCAGCA TGGATGGCTG ACTTTAAGAG GGGGGAAAGG GTTTCTCTTC 
ACCGTGGCTG ACCTCCAGGC TGGAGTTGTT CGCTATCATC ATGATGACAG CGACTCCACC 
AAAGACTTCG TGGTCTTCCG GATATTTGAT GGCCATCACA GCATCCGTCA CAAATTCCCC 
ATCAACGTCT TGCCCAAAGA TGATAGTCCC CCGTTCCTCA TAACCAATGT TGTGATTGAA 
CTGGAGGAGG GGCAGACCAT CCTGATCCAG GGATCCATGC TGCGAGCTTC AGATGTGGAC 
GCCAGTGATG ACTACATCTT CTTCAATATC ACAAAGCCTC CACAGGCTGG GGAGATCATG 
AAGAAGCCAG GGCCAGGACT GATAGGCTAT CCTGTCCATG GCTTCCTTCA GAGGGATTTG 
TTTAATGGAA TCATTTATTA TCGTCATTTT GGTGGAGAAA TCTTTGAAGA TTCTTTTCAA 
TTTGTCCTGT GGGACAGCCA TGAACCTCCA AATCTTTCAG TGCCACAGGT GGCAACAATC 
CATATAACTC CAGTGGATGA CCAGCTTCCA AAAGAGGCTC CTGGAGTTTC TCGGCATTTG 
GTTGTCAAGG AAACTGAGGT GGCCTATATA ACTAAGAAAC AGCTACATTT TATAGATTCA 
GAATCATATG ACAGGGAGCT GGTCTACACA ATAACTACTC CTCCATTTTT CTCCTTCAGC 
CACAGACACT TGGATGCTGG GAAATTATTT ATGGTGGACA GCATACCAAA AGTAGTTAAG 
AATCCTACGG CCCTGGAGCT GAGGTCATTC ACTCAGCATG CTGTGAACTA TATGAAAGTG 
GCCTACATGC CCCCCATGCA AGACATTGGT CCCCATTGCA GAGATGTCCA GTTCACATTT 
TCTGTCAGTA ACCAACATGG CGGTACTTTG CATGGGATCT GCTTTAACAT TACAATCCTC 
CCAGTGGACA ATCAAGTTCC TGAGGCGTTC ACCAACCCTC TGAAAGTGAC TGAGGGAGGT 
CAAAGCATCA TCAGCACAGA GCACATTCTA ATTTCTGATG CAGATACCAA GCTGGACAAT 
ATTGACCTCT CCCTGCGGGA ATTGCCTCTG CACGGAAGGG TGGAGCTGAA TGGATTTCCT 
CTAAATTCAG GGGGCACATT TTCTTGGGGC GATCTCCATA CCTTAAAAGT TAGGTATCAA 
CATGATGGAA CTGAAGTTCT TCAGGATGAC CTACTCTTGG AGGTCACCGA TGGCACAAAT 
TCAGCAGAAT TTGTACTACA TGTTGAGGTA TTCCCTGTCA ACGATGAGCC ACCAGTCTTA 
AAGGCTGACC TCATGCCTGT CATGAATTGC TCAGAGGGAG GAGAGGTGGT CATCACCTCT 
GAATACATTT TTGCTACTGA TGTGGACAGC GATAACTTGA AGTTGATGTT TGTGATTGCT 
CGCGAACCTC AGCATGGGGT GGTGAGGAGA GCTGGAGTCA CAGTGGATCA GTTCTCTCAG 
AGAGATGTTA TCTCAGAGGC CGTGACATAC AAACACACAG GTGGCGAGAT TGGCCTGATG 
CCTTGTTTTG ACACCATTAC ATTGGTGGTT TCGGATGGAG AGGCTGGCCC TTTTGTGAAT 
GGCTGTTGCT ACAATGGACC CAATCCATCT GTTCCACTTC ATGCGTCCTT TCCAGTGTAT 
GATCTCAACA TCACGGTATA CCCAGTAGAC AACCAGCCAC CTTCCATTGC AATAGGTCCC 
GTGTTTGTTG TAGATGAGGG CTGCTCAACA GCCCTTACTG TTAACCATTT GTCCGCCACT 
GACCCTGACA CTGCAGCAGA TGACTTGGAA TTTGTTTTGG TTTCTCCTCC TCAGTTTGGC 
TACCTCGAAA ATATACTCCC TTCTGTGGGT TTTGAAAAAA GCAATATTGG CATAAGTATA 
GATTCATTTC AGTGGAAAGA CATGAACGCT TTTCACATTA ACTATGTGCA GTCCAGGCAT 
CTGAGGATAG AACCAACTGC CGACCAGTTC ACGGTGTACG TCACAGATGG GAAGCATCAC 
TCCTTGGAGA TACCATTTTC TATTATAATC AACCCCACAA ATGATGAAGC TCCTGACTTT 
GTAGTGCAGA ATATTACTGT GTGTGAGGGT CAGATGAAAG AGCTGGACTC TTCCATCATC 
AGCGCTGTGG ACCTGGACAT TCCCCAGGAT GCCCTGCTGT TCAGCATCAC TCAAAAGCCA 
CGCCATGGCC TCCTCATCGA TAGGGGGTTT AGCAAAGACT TCTCTGAGAA TAAGCAGCCA 
GCCAACCCTC ACCAGAAACA TGCACCTGTT CACAGCTTTT CCATGGAACT CCTCAAGACT 
GGAATGAGGT TGACGTACAT GCATGATGAC TCAGAGAGCC TTGCTGATGA TTTTACAATC 
CAATTGTCAG ATGGGAAACA TAAGATACTT AAAACCATTT CAGTAGAGGT CATCCCAGTT 
AATGATGAAA AACCAATGCT GAGCAAGAAG GCTGAAATTG CAATGAATAT GGGTGAAACT 
CGTATTATTT CCAGTGCTAT TCTTTCAGCC ATAGATGAAG ACTCACCCAG GGAGAAGATT 
TACTATGTAT TTGAAAGGCT TCCCCAAAAT GGGCAACTTC AGCTTAAGAT AGGGAGGGAC 
TGGGTTCCTC TCTCCCCTGG CATGAAATGC ACTCAGGAGG AAGTGGATCT GAACTTGCTG 
AGATACACAC ACACCGGGGC AATGGATTCC CAGAATCAAG ATAGCTTCAC CTTCTACCTT 
TGGGATGGCA ACAACAGGTC CCCTGCTCTT GACTGTCAAA TCACCATCAA GGACATGGAA 
AAAGGTGATA TTGTCATCCT TACAAAACCA CTCGTGGTGT CTAAAGGTGA TAGAGGTTTC 
TTAACAACCA CCACGCTCCT GGCTGTGGAC GGAACAGACA AGCCTGAGGA ACTGCTCTAT 
GTCATCACCT CCCCTCCGCG ATATGGCCAG ATCGAATATG TTCACTATCC TGGAGTTCCC 
ATTACAAACT TCAGCCAAAT GGATGTAGTG GGGCAGACAG TTTGCTATGT ACACAAGAGC 
AAGGTGACTG TCTCCAGTGA CAGATTCAGA TTCATCATCA GCAATGGACT GCGGACCGAG 
CACGGGGTGT TTGAGATCAC ACTGGAGACT GTGGACAGAG CCCTGCCTGT GGTAACCAGG 
AACAAGGGGT TGAGACTGGC CCAAGGGGCC GTGGGCCTGC TTTCCCCTGA CCTCCTTCAG 
CTGACCGACC CTGATACACC TGCGGAGAAC CTCACCTTCC TCTTGGTTCA GCTCCCCCAG 
CATGGCCAGC TCTACCTGTG GGGGACAGGG CTACTTCAAC ACAATTTCAC CCAGCAGGAT 
GTGGACAGCA AGAATGTGGC CTATCGGCAC TCAGGAGGGG ACTCCCAGAC TGACTGCTTT 
ACTTTCATGG CCACAGATGG GACAAACCAA GGCTTTATTG TGAATGGGAG AGTGTGGGAA 
GAACCTGTTT TATTCACCAT TCAGGTAGAC CAATTGGACA AAACAGCTCC TCGTATCACA 
CTCTTGCATT CCCCTTCTCA AGTGGGGCTC CTGAAAAATG GCTGCTACGG GATTTACATC 
ACTTCCCGCG TGTTGAAGGC ATCAGACCCT GACACTGAGG ACGATCAGAT CATCTTTAAA 
ATTCTACAAG GCCCAAAACA TGGACATCTG GAGAACACAA CAACAGGTGA ATTTATCCAT 
GAGAAATTTA GCCAAAAGGA CTTAAACAGT AAGACTATTC TTTACATCAT AAACCCATCT 
TTGGAAGTAA ATTCAGATAC CGTGGAATTT CAAATCATGG ACCCCACAGG GAACTCGGCC 
ACTCCTCAAA TTTTGGAACT GAAGTGGTCT CATATTGAAT GGTCACAGAC CGAATATGAA 
GTCTGTGAGA ATGTGGGTTT GTTGCCCTTG GAAATTATCA GAAGGGGATA TTCCATGGAC 
TCGGCCTTTG TGGGTATAAA GGTCAACCAA GTGTCAGCTG CAGTTGGAAA AGATTTCACC 
GTGATTCCAT CTAAACTGAT TCAGTTTGAC CCAGGAATGT CAACTAAGAT GTGGAATATA 
GCAATTACCT ATGACGGATT AGAGGAAGAT GATGAGGTCT TTGAAGTAAT TCTGAACTCC 
CCTGTGAATG CAGTTCTTGG CACAAAGACA AAAGCTGCAG TGAAAATTTT GGACTCAAAA 
GGAGGACAAT GCCATCCTTC ATATTCCTCC AACCAAAGCA AGCACAGCAC ATGGGAGAAG 
GGCATTTGGC ATCTGCTGCC CCCAGGGTCT TCCTCATCCA CCACTTCTGG TTCCTTTCAT 
CTGGAAAGAA GACCTCTTCC ATCTTCCATG CAGCTAGCAG TCATCAGGGG AGACACCCTG 
CGGGGCTTTG ATTCTACAGA TCTTTCTCAA AGGAAGCTTA GGACCCGTGG GAATGGCAAA 
ACAGTTCGTC CATCCTCTGT TTATAGAAAT GGAACAGACA TCATCTATAA TTATCATGGG 
ATAGTTTCCT TGAAACTGGA GGATGACAGT TTCCCAACTC ACAAAAGGAA GGCCAAAGTA 
TCCATCATTA GTCAGCCACA AAAGACAATC AAAGTGGCAG AACTGCCTCA AGCAGATAAG 
GTGGAATCCA CAACTGACTC ACACTTCCCC AGACAGGACC AGTTGCCCTC ATTTCCAAAG 
AACTGCACTC TGGAATTAAA GGGACTCTTC CATTTTGAAG AAGGCATCCA GAAGCTGTAT 
CAGTGCAATG GGATCGCCTG GAAAGCCTGG AGTCCCCAAA CCAAGGATGT GGAAGACAAA 
TCCTGTCCAG CCGGGTGGCA CCAGCACTCA GGCTACTGTC ACATCTTGAT CACAGAGCAG 
AAAGGCACCT GGAATGCGGC TGCCCAAGCT TGCAGGGAAC AATACCTGGG CAACCTTGTA 
ACTGTATTCT CCAGGCAGCA CATGCGGTGG CTCTGGGACA TTGGTGGGAG AAAGTCCTTT 
TGGATAGGTT TGAACGACCA AGTGCATGCT GGCCACTGGG AGTGGATCGG TGGTGAACCT 
GTTGCCTTCA CCAATGGGAG AAGAGGGCCC TCTCAACGCT CCAAGCTTGG AAAGAGCTGT 
GTTTTGGTTC AAAGACAAGG GAAATGGCAA ACAAAAGACT GTAGGAGAGC CAAACCTCAT 
AATTATGTGT GTTCCAGAAA ACTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016869805.1
CDS	1065..7631
Translation	MNSLSWGAANAVLLLLLLAWASPTFISINRGVRVMKGHSAFLSGDDLKFAIPKEKDACKVEVVMNEPITQRVGKLTPQVFDCHFLPNEVKYVHNGCPILDEDTVKLRLYRFTERDTFIETFILWVYLLEPDCNIIHMSNNVLEVPEFNGLSQAIDKNLLRFDYDRMASLECTVSLDTARTRLPAHGQMVLGEPRPEEPRGDQPHSFFPESRMMSFNAFLELRAKLKCPGGSCTPGLKKIGSLKVSCEEFLLMGLRYQHLDPPSPNIDYISIQLDLTDTRSKIVYKSESAWLPVYIRAGIPNQIPKAAFMAVFILEVDQFILTSLTTSVLDCEEDETPKPLLVFNITKAPLQGYVTHLLDHTRPISSFTWKDLSDMQIAYQPPNSSHSERRHDEVELEVYDFFFERSAPMTVHISIRTADTNAPRVSWNTGLSLLEGQSRAITWEQFQVVDNDDIGAVRLVTVGGLQHGWLTLRGGKGFLFTVADLQAGVVRYHHDDSDSTKDFVVFRIFDGHHSIRHKFPINVLPKDDSPPFLITNVVIELEEGQTILIQGSMLRASDVDASDDYIFFNITKPPQAGEIMKKPGPGLIGYPVHGFLQRDLFNGIIYYRHFGGEIFEDSFQFVLWDSHEPPNLSVPQVATIHITPVDDQLPKEAPGVSRHLVVKETEVAYITKKQLHFIDSESYDRELVYTITTPPFFSFSHRHLDAGKLFMVDSIPKVVKNPTALELRSFTQHAVNYMKVAYMPPMQDIGPHCRDVQFTFSVSNQHGGTLHGICFNITILPVDNQVPEAFTNPLKVTEGGQSIISTEHILISDADTKLDNIDLSLRELPLHGRVELNGFPLNSGGTFSWGDLHTLKVRYQHDGTEVLQDDLLLEVTDGTNSAEFVLHVEVFPVNDEPPVLKADLMPVMNCSEGGEVVITSEYIFATDVDSDNLKLMFVIAREPQHGVVRRAGVTVDQFSQRDVISEAVTYKHTGGEIGLMPCFDTITLVVSDGEAGPFVNGCCYNGPNPSVPLHASFPVYDLNITVYPVDNQPPSIAIGPVFVVDEGCSTALTVNHLSATDPDTAADDLEFVLVSPPQFGYLENILPSVGFEKSNIGISIDSFQWKDMNAFHINYVQSRHLRIEPTADQFTVYVTDGKHHSLEIPFSIIINPTNDEAPDFVVQNITVCEGQMKELDSSIISAVDLDIPQDALLFSITQKPRHGLLIDRGFSKDFSENKQPANPHQKHAPVHSFSMELLKTGMRLTYMHDDSESLADDFTIQLSDGKHKILKTISVEVIPVNDEKPMLSKKAEIAMNMGETRIISSAILSAIDEDSPREKIYYVFERLPQNGQLQLKIGRDWVPLSPGMKCTQEEVDLNLLRYTHTGAMDSQNQDSFTFYLWDGNNRSPALDCQITIKDMEKGDIVILTKPLVVSKGDRGFLTTTTLLAVDGTDKPEELLYVITSPPRYGQIEYVHYPGVPITNFSQMDVVGQTVCYVHKSKVTVSSDRFRFIISNGLRTEHGVFEITLETVDRALPVVTRNKGLRLAQGAVGLLSPDLLQLTDPDTPAENLTFLLVQLPQHGQLYLWGTGLLQHNFTQQDVDSKNVAYRHSGGDSQTDCFTFMATDGTNQGFIVNGRVWEEPVLFTIQVDQLDKTAPRITLLHSPSQVGLLKNGCYGIYITSRVLKASDPDTEDDQIIFKILQGPKHGHLENTTTGEFIHEKFSQKDLNSKTILYIINPSLEVNSDTVEFQIMDPTGNSATPQILELKWSHIEWSQTEYEVCENVGLLPLEIIRRGYSMDSAFVGIKVNQVSAAVGKDFTVIPSKLIQFDPGMSTKMWNIAITYDGLEEDDEVFEVILNSPVNAVLGTKTKAAVKILDSKGGQCHPSYSSNQSKHSTWEKGIWHLLPPGSSSSTTSGSFHLERRPLPSSMQLAVIRGDTLRGFDSTDLSQRKLRTRGNGKTVRPSSVYRNGTDIIYNYHGIVSLKLEDDSFPTHKRKAKVSIISQPQKTIKVAELPQADKVESTTDSHFPRQDQLPSFPKNCTLELKGLFHFEEGIQKLYQCNGIAWKAWSPQTKDVEDKSCPAGWHQHSGYCHILITEQKGTWNAAAQACREQYLGNLVTVFSRQHMRWLWDIGGRKSFWIGLNDQVHAGHWEWIGGEPVAFTNGRRGPSQRSKLGKSCVLVQRQGKWQTKDCRRAKPHNYVCSRKL

Target ORF information:

RefSeq Version	XM_017014316.1
Organism	Homo sapiens(human)
Definition	Homo sapiens FRAS1 related extracellular matrix 1 (FREM1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017014316.1

ORF Insert Sequence:

ATGAACTCTC TGAGTTGGGG GGCTGCGAAT GCCGTGCTGC TGCTGCTCCT CCTGGCCTGG 
GCCAGCCCCA CCTTCATCAG CATCAACCGC GGGGTGAGGG TGATGAAGGG CCACTCTGCC 
TTCCTGTCAG GAGATGACCT GAAGTTTGCC ATCCCTAAAG AGAAAGATGC CTGCAAAGTG 
GAAGTTGTGA TGAATGAGCC AATAACCCAG AGGGTTGGGA AACTCACTCC ACAGGTCTTT 
GACTGCCATT TCCTTCCCAA CGAAGTCAAG TATGTTCACA ATGGTTGTCC AATTCTTGAT 
GAAGACACAG TGAAGCTCAG ACTTTACAGA TTTACTGAAA GAGATACCTT CATAGAAACT 
TTTATCCTGT GGGTCTATCT CCTGGAACCA GACTGTAACA TCATCCATAT GAGTAACAAT 
GTGCTGGAGG TGCCTGAATT CAATGGCTTG TCCCAAGCGA TTGATAAAAA TCTGCTCAGA 
TTCGATTATG ATAGGATGGC TAGCCTGGAA TGTACCGTCA GCCTGGACAC TGCGAGAACT 
CGGCTGCCAG CCCATGGCCA GATGGTTCTC GGGGAGCCTC GACCAGAAGA ACCTCGTGGA 
GATCAGCCAC ACAGTTTTTT CCCAGAGTCC CGTATGATGT CTTTTAATGC CTTTCTGGAA 
CTGAGAGCAA AACTGAAGTG TCCAGGTGGG AGCTGTACCC CAGGATTAAA GAAAATAGGA 
AGCCTCAAAG TGAGCTGTGA GGAGTTCCTG CTGATGGGCC TTCGTTATCA GCATCTGGAT 
CCCCCTTCAC CCAACATTGA TTATATCTCC ATCCAACTGG ACCTCACAGA TACCAGGAGC 
AAAATTGTGT ACAAGTCAGA GAGTGCGTGG CTGCCTGTCT ATATCAGAGC TGGAATTCCG 
AATCAGATTC CAAAGGCTGC ATTCATGGCC GTGTTTATTC TGGAAGTGGA TCAGTTCATC 
CTGACCTCCT TGACTACATC AGTTCTGGAC TGTGAAGAAG ATGAGACCCC TAAACCCTTG 
CTGGTGTTCA ACATTACTAA AGCCCCGCTC CAGGGCTATG TGACTCACCT GTTGGATCAC 
ACCAGACCAA TCTCCTCATT CACCTGGAAA GATCTCAGTG ACATGCAGAT CGCCTATCAG 
CCACCAAACA GCAGCCATTC TGAGAGGAGA CATGATGAGG TAGAATTGGA GGTATACGAC 
TTCTTCTTTG AAAGGAGTGC ACCTATGACA GTCCACATCT CCATCAGAAC AGCAGATACA 
AATGCCCCCC GTGTATCCTG GAATACAGGT CTGAGTCTCC TTGAGGGGCA GTCTCGAGCC 
ATCACTTGGG AACAGTTTCA GGTTGTCGAC AATGACGACA TTGGTGCTGT CCGGCTAGTC 
ACCGTTGGTG GCCTGCAGCA TGGATGGCTG ACTTTAAGAG GGGGGAAAGG GTTTCTCTTC 
ACCGTGGCTG ACCTCCAGGC TGGAGTTGTT CGCTATCATC ATGATGACAG CGACTCCACC 
AAAGACTTCG TGGTCTTCCG GATATTTGAT GGCCATCACA GCATCCGTCA CAAATTCCCC 
ATCAACGTCT TGCCCAAAGA TGATAGTCCC CCGTTCCTCA TAACCAATGT TGTGATTGAA 
CTGGAGGAGG GGCAGACCAT CCTGATCCAG GGATCCATGC TGCGAGCTTC AGATGTGGAC 
GCCAGTGATG ACTACATCTT CTTCAATATC ACAAAGCCTC CACAGGCTGG GGAGATCATG 
AAGAAGCCAG GGCCAGGACT GATAGGCTAT CCTGTCCATG GCTTCCTTCA GAGGGATTTG 
TTTAATGGAA TCATTTATTA TCGTCATTTT GGTGGAGAAA TCTTTGAAGA TTCTTTTCAA 
TTTGTCCTGT GGGACAGCCA TGAACCTCCA AATCTTTCAG TGCCACAGGT GGCAACAATC 
CATATAACTC CAGTGGATGA CCAGCTTCCA AAAGAGGCTC CTGGAGTTTC TCGGCATTTG 
GTTGTCAAGG AAACTGAGGT GGCCTATATA ACTAAGAAAC AGCTACATTT TATAGATTCA 
GAATCATATG ACAGGGAGCT GGTCTACACA ATAACTACTC CTCCATTTTT CTCCTTCAGC 
CACAGACACT TGGATGCTGG GAAATTATTT ATGGTGGACA GCATACCAAA AGTAGTTAAG 
AATCCTACGG CCCTGGAGCT GAGGTCATTC ACTCAGCATG CTGTGAACTA TATGAAAGTG 
GCCTACATGC CCCCCATGCA AGACATTGGT CCCCATTGCA GAGATGTCCA GTTCACATTT 
TCTGTCAGTA ACCAACATGG CGGTACTTTG CATGGGATCT GCTTTAACAT TACAATCCTC 
CCAGTGGACA ATCAAGTTCC TGAGGCGTTC ACCAACCCTC TGAAAGTGAC TGAGGGAGGT 
CAAAGCATCA TCAGCACAGA GCACATTCTA ATTTCTGATG CAGATACCAA GCTGGACAAT 
ATTGACCTCT CCCTGCGGGA ATTGCCTCTG CACGGAAGGG TGGAGCTGAA TGGATTTCCT 
CTAAATTCAG GGGGCACATT TTCTTGGGGC GATCTCCATA CCTTAAAAGT TAGGTATCAA 
CATGATGGAA CTGAAGTTCT TCAGGATGAC CTACTCTTGG AGGTCACCGA TGGCACAAAT 
TCAGCAGAAT TTGTACTACA TGTTGAGGTA TTCCCTGTCA ACGATGAGCC ACCAGTCTTA 
AAGGCTGACC TCATGCCTGT CATGAATTGC TCAGAGGGAG GAGAGGTGGT CATCACCTCT 
GAATACATTT TTGCTACTGA TGTGGACAGC GATAACTTGA AGTTGATGTT TGTGATTGCT 
CGCGAACCTC AGCATGGGGT GGTGAGGAGA GCTGGAGTCA CAGTGGATCA GTTCTCTCAG 
AGAGATGTTA TCTCAGAGGC CGTGACATAC AAACACACAG GTGGCGAGAT TGGCCTGATG 
CCTTGTTTTG ACACCATTAC ATTGGTGGTT TCGGATGGAG AGGCTGGCCC TTTTGTGAAT 
GGCTGTTGCT ACAATGGACC CAATCCATCT GTTCCACTTC ATGCGTCCTT TCCAGTGTAT 
GATCTCAACA TCACGGTATA CCCAGTAGAC AACCAGCCAC CTTCCATTGC AATAGGTCCC 
GTGTTTGTTG TAGATGAGGG CTGCTCAACA GCCCTTACTG TTAACCATTT GTCCGCCACT 
GACCCTGACA CTGCAGCAGA TGACTTGGAA TTTGTTTTGG TTTCTCCTCC TCAGTTTGGC 
TACCTCGAAA ATATACTCCC TTCTGTGGGT TTTGAAAAAA GCAATATTGG CATAAGTATA 
GATTCATTTC AGTGGAAAGA CATGAACGCT TTTCACATTA ACTATGTGCA GTCCAGGCAT 
CTGAGGATAG AACCAACTGC CGACCAGTTC ACGGTGTACG TCACAGATGG GAAGCATCAC 
TCCTTGGAGA TACCATTTTC TATTATAATC AACCCCACAA ATGATGAAGC TCCTGACTTT 
GTAGTGCAGA ATATTACTGT GTGTGAGGGT CAGATGAAAG AGCTGGACTC TTCCATCATC 
AGCGCTGTGG ACCTGGACAT TCCCCAGGAT GCCCTGCTGT TCAGCATCAC TCAAAAGCCA 
CGCCATGGCC TCCTCATCGA TAGGGGGTTT AGCAAAGACT TCTCTGAGAA TAAGCAGCCA 
GCCAACCCTC ACCAGAAACA TGCACCTGTT CACAGCTTTT CCATGGAACT CCTCAAGACT 
GGAATGAGGT TGACGTACAT GCATGATGAC TCAGAGAGCC TTGCTGATGA TTTTACAATC 
CAATTGTCAG ATGGGAAACA TAAGATACTT AAAACCATTT CAGTAGAGGT CATCCCAGTT 
AATGATGAAA AACCAATGCT GAGCAAGAAG GCTGAAATTG CAATGAATAT GGGTGAAACT 
CGTATTATTT CCAGTGCTAT TCTTTCAGCC ATAGATGAAG ACTCACCCAG GGAGAAGATT 
TACTATGTAT TTGAAAGGCT TCCCCAAAAT GGGCAACTTC AGCTTAAGAT AGGGAGGGAC 
TGGGTTCCTC TCTCCCCTGG CATGAAATGC ACTCAGGAGG AAGTGGATCT GAACTTGCTG 
AGATACACAC ACACCGGGGC AATGGATTCC CAGAATCAAG ATAGCTTCAC CTTCTACCTT 
TGGGATGGCA ACAACAGGTC CCCTGCTCTT GACTGTCAAA TCACCATCAA GGACATGGAA 
AAAGGTGATA TTGTCATCCT TACAAAACCA CTCGTGGTGT CTAAAGGTGA TAGAGGTTTC 
TTAACAACCA CCACGCTCCT GGCTGTGGAC GGAACAGACA AGCCTGAGGA ACTGCTCTAT 
GTCATCACCT CCCCTCCGCG ATATGGCCAG ATCGAATATG TTCACTATCC TGGAGTTCCC 
ATTACAAACT TCAGCCAAAT GGATGTAGTG GGGCAGACAG TTTGCTATGT ACACAAGAGC 
AAGGTGACTG TCTCCAGTGA CAGATTCAGA TTCATCATCA GCAATGGACT GCGGACCGAG 
CACGGGGTGT TTGAGATCAC ACTGGAGACT GTGGACAGAG CCCTGCCTGT GGTAACCAGG 
AACAAGGGGT TGAGACTGGC CCAAGGGGCC GTGGGCCTGC TTTCCCCTGA CCTCCTTCAG 
CTGACCGACC CTGATACACC TGCGGAGAAC CTCACCTTCC TCTTGGTTCA GCTCCCCCAG 
CATGGCCAGC TCTACCTGTG GGGGACAGGG CTACTTCAAC ACAATTTCAC CCAGCAGGAT 
GTGGACAGCA AGAATGTGGC CTATCGGCAC TCAGGAGGGG ACTCCCAGAC TGACTGCTTT 
ACTTTCATGG CCACAGATGG GACAAACCAA GGCTTTATTG TGAATGGGAG AGTGTGGGAA 
GAACCTGTTT TATTCACCAT TCAGGTAGAC CAATTGGACA AAACAGCTCC TCGTATCACA 
CTCTTGCATT CCCCTTCTCA AGTGGGGCTC CTGAAAAATG GCTGCTACGG GATTTACATC 
ACTTCCCGCG TGTTGAAGGC ATCAGACCCT GACACTGAGG ACGATCAGAT CATCTTTAAA 
ATTCTACAAG GCCCAAAACA TGGACATCTG GAGAACACAA CAACAGGTGA ATTTATCCAT 
GAGAAATTTA GCCAAAAGGA CTTAAACAGT AAGACTATTC TTTACATCAT AAACCCATCT 
TTGGAAGTAA ATTCAGATAC CGTGGAATTT CAAATCATGG ACCCCACAGG GAACTCGGCC 
ACTCCTCAAA TTTTGGAACT GAAGTGGTCT CATATTGAAT GGTCACAGAC CGAATATGAA 
GTCTGTGAGA ATGTGGGTTT GTTGCCCTTG GAAATTATCA GAAGGGGATA TTCCATGGAC 
TCGGCCTTTG TGGGTATAAA GGTCAACCAA GTGTCAGCTG CAGTTGGAAA AGATTTCACC 
GTGATTCCAT CTAAACTGAT TCAGTTTGAC CCAGGAATGT CAACTAAGAT GTGGAATATA 
GCAATTACCT ATGACGGATT AGAGGAAGAT GATGAGGTCT TTGAAGTAAT TCTGAACTCC 
CCTGTGAATG CAGTTCTTGG CACAAAGACA AAAGCTGCAG TGAAAATTTT GGACTCAAAA 
GGAGGACAAT GCCATCCTTC ATATTCCTCC AACCAAAGCA AGCACAGCAC ATGGGAGAAG 
GGCATTTGGC ATCTGCTGCC CCCAGGGTCT TCCTCATCCA CCACTTCTGG TTCCTTTCAT 
CTGGAAAGAA GACCTCTTCC ATCTTCCATG CAGCTAGCAG TCATCAGGGG AGACACCCTG 
CGGGGCTTTG ATTCTACAGA TCTTTCTCAA AGGAAGCTTA GGACCCGTGG GAATGGCAAA 
ACAGTTCGTC CATCCTCTGT TTATAGAAAT GGAACAGACA TCATCTATAA TTATCATGGG 
ATAGTTTCCT TGAAACTGGA GGATGACAGT TTCCCAACTC ACAAAAGGAA GGCCAAAGTA 
TCCATCATTA GTCAGCCACA AAAGACAATC AAAGTGGCAG AACTGCCTCA AGCAGATAAG 
GTGGAATCCA CAACTGACTC ACACTTCCCC AGACAGGACC AGTTGCCCTC ATTTCCAAAG 
AACTGCACTC TGGAATTAAA GGGACTCTTC CATTTTGAAG AAGGCATCCA GAAGCTGTAT 
CAGTGCAATG GGATCGCCTG GAAAGCCTGG AGTCCCCAAA CCAAGGATGT GGAAGACAAA 
TCCTGTCCAG CCGGGTGGCA CCAGCACTCA GGCTACTGTC ACATCTTGAT CACAGAGCAG 
AAAGGCACCT GGAATGCGGC TGCCCAAGCT TGCAGGGAAC AATACCTGGG CAACCTTGTA 
ACTGTATTCT CCAGGCAGCA CATGCGGTGG CTCTGGGACA TTGGTGGGAG AAAGTCCTTT 
TGGATAGGTT TGAACGACCA AGTGCATGCT GGCCACTGGG AGTGGATCGG TGGTGAACCT 
GTTGCCTTCA CCAATGGGAG AAGAGGGCCC TCTCAACGCT CCAAGCTTGG AAAGAGCTGT 
GTTTTGGTTC AAAGACAAGG GAAATGGCAA ACAAAAGACT GTAGGAGAGC CAAACCTCAT 
AATTATGTGT GTTCCAGAAA ACTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FREM1 ( XM_017014316.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016869805.1 Homo sapiens FRAS1 related extracellular matrix 1 (FREM1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu98459
Clone ID Related Accession (Same CDS sequence)	XM_017014320.1 , XM_017014318.1 , XM_017014316.1 , XM_017014316.2 , XM_017014317.1 , XM_017014319.2 , XM_017014321.2 , XM_017014323.1 , XM_017014322.1 , XM_017014321.1 , XM_017014320.2 , XM_017014319.1
Accession Version	XM_017014316.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6567bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	FRAS1-related extracellular matrix protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017014316.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACTCTC TGAGTTGGGG GGCTGCGAAT GCCGTGCTGC TGCTGCTCCT CCTGGCCTGG 
GCCAGCCCCA CCTTCATCAG CATCAACCGC GGGGTGAGGG TGATGAAGGG CCACTCTGCC 
TTCCTGTCAG GAGATGACCT GAAGTTTGCC ATCCCTAAAG AGAAAGATGC CTGCAAAGTG 
GAAGTTGTGA TGAATGAGCC AATAACCCAG AGGGTTGGGA AACTCACTCC ACAGGTCTTT 
GACTGCCATT TCCTTCCCAA CGAAGTCAAG TATGTTCACA ATGGTTGTCC AATTCTTGAT 
GAAGACACAG TGAAGCTCAG ACTTTACAGA TTTACTGAAA GAGATACCTT CATAGAAACT 
TTTATCCTGT GGGTCTATCT CCTGGAACCA GACTGTAACA TCATCCATAT GAGTAACAAT 
GTGCTGGAGG TGCCTGAATT CAATGGCTTG TCCCAAGCGA TTGATAAAAA TCTGCTCAGA 
TTCGATTATG ATAGGATGGC TAGCCTGGAA TGTACCGTCA GCCTGGACAC TGCGAGAACT 
CGGCTGCCAG CCCATGGCCA GATGGTTCTC GGGGAGCCTC GACCAGAAGA ACCTCGTGGA 
GATCAGCCAC ACAGTTTTTT CCCAGAGTCC CGTATGATGT CTTTTAATGC CTTTCTGGAA 
CTGAGAGCAA AACTGAAGTG TCCAGGTGGG AGCTGTACCC CAGGATTAAA GAAAATAGGA 
AGCCTCAAAG TGAGCTGTGA GGAGTTCCTG CTGATGGGCC TTCGTTATCA GCATCTGGAT 
CCCCCTTCAC CCAACATTGA TTATATCTCC ATCCAACTGG ACCTCACAGA TACCAGGAGC 
AAAATTGTGT ACAAGTCAGA GAGTGCGTGG CTGCCTGTCT ATATCAGAGC TGGAATTCCG 
AATCAGATTC CAAAGGCTGC ATTCATGGCC GTGTTTATTC TGGAAGTGGA TCAGTTCATC 
CTGACCTCCT TGACTACATC AGTTCTGGAC TGTGAAGAAG ATGAGACCCC TAAACCCTTG 
CTGGTGTTCA ACATTACTAA AGCCCCGCTC CAGGGCTATG TGACTCACCT GTTGGATCAC 
ACCAGACCAA TCTCCTCATT CACCTGGAAA GATCTCAGTG ACATGCAGAT CGCCTATCAG 
CCACCAAACA GCAGCCATTC TGAGAGGAGA CATGATGAGG TAGAATTGGA GGTATACGAC 
TTCTTCTTTG AAAGGAGTGC ACCTATGACA GTCCACATCT CCATCAGAAC AGCAGATACA 
AATGCCCCCC GTGTATCCTG GAATACAGGT CTGAGTCTCC TTGAGGGGCA GTCTCGAGCC 
ATCACTTGGG AACAGTTTCA GGTTGTCGAC AATGACGACA TTGGTGCTGT CCGGCTAGTC 
ACCGTTGGTG GCCTGCAGCA TGGATGGCTG ACTTTAAGAG GGGGGAAAGG GTTTCTCTTC 
ACCGTGGCTG ACCTCCAGGC TGGAGTTGTT CGCTATCATC ATGATGACAG CGACTCCACC 
AAAGACTTCG TGGTCTTCCG GATATTTGAT GGCCATCACA GCATCCGTCA CAAATTCCCC 
ATCAACGTCT TGCCCAAAGA TGATAGTCCC CCGTTCCTCA TAACCAATGT TGTGATTGAA 
CTGGAGGAGG GGCAGACCAT CCTGATCCAG GGATCCATGC TGCGAGCTTC AGATGTGGAC 
GCCAGTGATG ACTACATCTT CTTCAATATC ACAAAGCCTC CACAGGCTGG GGAGATCATG 
AAGAAGCCAG GGCCAGGACT GATAGGCTAT CCTGTCCATG GCTTCCTTCA GAGGGATTTG 
TTTAATGGAA TCATTTATTA TCGTCATTTT GGTGGAGAAA TCTTTGAAGA TTCTTTTCAA 
TTTGTCCTGT GGGACAGCCA TGAACCTCCA AATCTTTCAG TGCCACAGGT GGCAACAATC 
CATATAACTC CAGTGGATGA CCAGCTTCCA AAAGAGGCTC CTGGAGTTTC TCGGCATTTG 
GTTGTCAAGG AAACTGAGGT GGCCTATATA ACTAAGAAAC AGCTACATTT TATAGATTCA 
GAATCATATG ACAGGGAGCT GGTCTACACA ATAACTACTC CTCCATTTTT CTCCTTCAGC 
CACAGACACT TGGATGCTGG GAAATTATTT ATGGTGGACA GCATACCAAA AGTAGTTAAG 
AATCCTACGG CCCTGGAGCT GAGGTCATTC ACTCAGCATG CTGTGAACTA TATGAAAGTG 
GCCTACATGC CCCCCATGCA AGACATTGGT CCCCATTGCA GAGATGTCCA GTTCACATTT 
TCTGTCAGTA ACCAACATGG CGGTACTTTG CATGGGATCT GCTTTAACAT TACAATCCTC 
CCAGTGGACA ATCAAGTTCC TGAGGCGTTC ACCAACCCTC TGAAAGTGAC TGAGGGAGGT 
CAAAGCATCA TCAGCACAGA GCACATTCTA ATTTCTGATG CAGATACCAA GCTGGACAAT 
ATTGACCTCT CCCTGCGGGA ATTGCCTCTG CACGGAAGGG TGGAGCTGAA TGGATTTCCT 
CTAAATTCAG GGGGCACATT TTCTTGGGGC GATCTCCATA CCTTAAAAGT TAGGTATCAA 
CATGATGGAA CTGAAGTTCT TCAGGATGAC CTACTCTTGG AGGTCACCGA TGGCACAAAT 
TCAGCAGAAT TTGTACTACA TGTTGAGGTA TTCCCTGTCA ACGATGAGCC ACCAGTCTTA 
AAGGCTGACC TCATGCCTGT CATGAATTGC TCAGAGGGAG GAGAGGTGGT CATCACCTCT 
GAATACATTT TTGCTACTGA TGTGGACAGC GATAACTTGA AGTTGATGTT TGTGATTGCT 
CGCGAACCTC AGCATGGGGT GGTGAGGAGA GCTGGAGTCA CAGTGGATCA GTTCTCTCAG 
AGAGATGTTA TCTCAGAGGC CGTGACATAC AAACACACAG GTGGCGAGAT TGGCCTGATG 
CCTTGTTTTG ACACCATTAC ATTGGTGGTT TCGGATGGAG AGGCTGGCCC TTTTGTGAAT 
GGCTGTTGCT ACAATGGACC CAATCCATCT GTTCCACTTC ATGCGTCCTT TCCAGTGTAT 
GATCTCAACA TCACGGTATA CCCAGTAGAC AACCAGCCAC CTTCCATTGC AATAGGTCCC 
GTGTTTGTTG TAGATGAGGG CTGCTCAACA GCCCTTACTG TTAACCATTT GTCCGCCACT 
GACCCTGACA CTGCAGCAGA TGACTTGGAA TTTGTTTTGG TTTCTCCTCC TCAGTTTGGC 
TACCTCGAAA ATATACTCCC TTCTGTGGGT TTTGAAAAAA GCAATATTGG CATAAGTATA 
GATTCATTTC AGTGGAAAGA CATGAACGCT TTTCACATTA ACTATGTGCA GTCCAGGCAT 
CTGAGGATAG AACCAACTGC CGACCAGTTC ACGGTGTACG TCACAGATGG GAAGCATCAC 
TCCTTGGAGA TACCATTTTC TATTATAATC AACCCCACAA ATGATGAAGC TCCTGACTTT 
GTAGTGCAGA ATATTACTGT GTGTGAGGGT CAGATGAAAG AGCTGGACTC TTCCATCATC 
AGCGCTGTGG ACCTGGACAT TCCCCAGGAT GCCCTGCTGT TCAGCATCAC TCAAAAGCCA 
CGCCATGGCC TCCTCATCGA TAGGGGGTTT AGCAAAGACT TCTCTGAGAA TAAGCAGCCA 
GCCAACCCTC ACCAGAAACA TGCACCTGTT CACAGCTTTT CCATGGAACT CCTCAAGACT 
GGAATGAGGT TGACGTACAT GCATGATGAC TCAGAGAGCC TTGCTGATGA TTTTACAATC 
CAATTGTCAG ATGGGAAACA TAAGATACTT AAAACCATTT CAGTAGAGGT CATCCCAGTT 
AATGATGAAA AACCAATGCT GAGCAAGAAG GCTGAAATTG CAATGAATAT GGGTGAAACT 
CGTATTATTT CCAGTGCTAT TCTTTCAGCC ATAGATGAAG ACTCACCCAG GGAGAAGATT 
TACTATGTAT TTGAAAGGCT TCCCCAAAAT GGGCAACTTC AGCTTAAGAT AGGGAGGGAC 
TGGGTTCCTC TCTCCCCTGG CATGAAATGC ACTCAGGAGG AAGTGGATCT GAACTTGCTG 
AGATACACAC ACACCGGGGC AATGGATTCC CAGAATCAAG ATAGCTTCAC CTTCTACCTT 
TGGGATGGCA ACAACAGGTC CCCTGCTCTT GACTGTCAAA TCACCATCAA GGACATGGAA 
AAAGGTGATA TTGTCATCCT TACAAAACCA CTCGTGGTGT CTAAAGGTGA TAGAGGTTTC 
TTAACAACCA CCACGCTCCT GGCTGTGGAC GGAACAGACA AGCCTGAGGA ACTGCTCTAT 
GTCATCACCT CCCCTCCGCG ATATGGCCAG ATCGAATATG TTCACTATCC TGGAGTTCCC 
ATTACAAACT TCAGCCAAAT GGATGTAGTG GGGCAGACAG TTTGCTATGT ACACAAGAGC 
AAGGTGACTG TCTCCAGTGA CAGATTCAGA TTCATCATCA GCAATGGACT GCGGACCGAG 
CACGGGGTGT TTGAGATCAC ACTGGAGACT GTGGACAGAG CCCTGCCTGT GGTAACCAGG 
AACAAGGGGT TGAGACTGGC CCAAGGGGCC GTGGGCCTGC TTTCCCCTGA CCTCCTTCAG 
CTGACCGACC CTGATACACC TGCGGAGAAC CTCACCTTCC TCTTGGTTCA GCTCCCCCAG 
CATGGCCAGC TCTACCTGTG GGGGACAGGG CTACTTCAAC ACAATTTCAC CCAGCAGGAT 
GTGGACAGCA AGAATGTGGC CTATCGGCAC TCAGGAGGGG ACTCCCAGAC TGACTGCTTT 
ACTTTCATGG CCACAGATGG GACAAACCAA GGCTTTATTG TGAATGGGAG AGTGTGGGAA 
GAACCTGTTT TATTCACCAT TCAGGTAGAC CAATTGGACA AAACAGCTCC TCGTATCACA 
CTCTTGCATT CCCCTTCTCA AGTGGGGCTC CTGAAAAATG GCTGCTACGG GATTTACATC 
ACTTCCCGCG TGTTGAAGGC ATCAGACCCT GACACTGAGG ACGATCAGAT CATCTTTAAA 
ATTCTACAAG GCCCAAAACA TGGACATCTG GAGAACACAA CAACAGGTGA ATTTATCCAT 
GAGAAATTTA GCCAAAAGGA CTTAAACAGT AAGACTATTC TTTACATCAT AAACCCATCT 
TTGGAAGTAA ATTCAGATAC CGTGGAATTT CAAATCATGG ACCCCACAGG GAACTCGGCC 
ACTCCTCAAA TTTTGGAACT GAAGTGGTCT CATATTGAAT GGTCACAGAC CGAATATGAA 
GTCTGTGAGA ATGTGGGTTT GTTGCCCTTG GAAATTATCA GAAGGGGATA TTCCATGGAC 
TCGGCCTTTG TGGGTATAAA GGTCAACCAA GTGTCAGCTG CAGTTGGAAA AGATTTCACC 
GTGATTCCAT CTAAACTGAT TCAGTTTGAC CCAGGAATGT CAACTAAGAT GTGGAATATA 
GCAATTACCT ATGACGGATT AGAGGAAGAT GATGAGGTCT TTGAAGTAAT TCTGAACTCC 
CCTGTGAATG CAGTTCTTGG CACAAAGACA AAAGCTGCAG TGAAAATTTT GGACTCAAAA 
GGAGGACAAT GCCATCCTTC ATATTCCTCC AACCAAAGCA AGCACAGCAC ATGGGAGAAG 
GGCATTTGGC ATCTGCTGCC CCCAGGGTCT TCCTCATCCA CCACTTCTGG TTCCTTTCAT 
CTGGAAAGAA GACCTCTTCC ATCTTCCATG CAGCTAGCAG TCATCAGGGG AGACACCCTG 
CGGGGCTTTG ATTCTACAGA TCTTTCTCAA AGGAAGCTTA GGACCCGTGG GAATGGCAAA 
ACAGTTCGTC CATCCTCTGT TTATAGAAAT GGAACAGACA TCATCTATAA TTATCATGGG 
ATAGTTTCCT TGAAACTGGA GGATGACAGT TTCCCAACTC ACAAAAGGAA GGCCAAAGTA 
TCCATCATTA GTCAGCCACA AAAGACAATC AAAGTGGCAG AACTGCCTCA AGCAGATAAG 
GTGGAATCCA CAACTGACTC ACACTTCCCC AGACAGGACC AGTTGCCCTC ATTTCCAAAG 
AACTGCACTC TGGAATTAAA GGGACTCTTC CATTTTGAAG AAGGCATCCA GAAGCTGTAT 
CAGTGCAATG GGATCGCCTG GAAAGCCTGG AGTCCCCAAA CCAAGGATGT GGAAGACAAA 
TCCTGTCCAG CCGGGTGGCA CCAGCACTCA GGCTACTGTC ACATCTTGAT CACAGAGCAG 
AAAGGCACCT GGAATGCGGC TGCCCAAGCT TGCAGGGAAC AATACCTGGG CAACCTTGTA 
ACTGTATTCT CCAGGCAGCA CATGCGGTGG CTCTGGGACA TTGGTGGGAG AAAGTCCTTT 
TGGATAGGTT TGAACGACCA AGTGCATGCT GGCCACTGGG AGTGGATCGG TGGTGAACCT 
GTTGCCTTCA CCAATGGGAG AAGAGGGCCC TCTCAACGCT CCAAGCTTGG AAAGAGCTGT 
GTTTTGGTTC AAAGACAAGG GAAATGGCAA ACAAAAGACT GTAGGAGAGC CAAACCTCAT 
AATTATGTGT GTTCCAGAAA ACTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016869805.1
CDS	787..7353
Translation	MNSLSWGAANAVLLLLLLAWASPTFISINRGVRVMKGHSAFLSGDDLKFAIPKEKDACKVEVVMNEPITQRVGKLTPQVFDCHFLPNEVKYVHNGCPILDEDTVKLRLYRFTERDTFIETFILWVYLLEPDCNIIHMSNNVLEVPEFNGLSQAIDKNLLRFDYDRMASLECTVSLDTARTRLPAHGQMVLGEPRPEEPRGDQPHSFFPESRMMSFNAFLELRAKLKCPGGSCTPGLKKIGSLKVSCEEFLLMGLRYQHLDPPSPNIDYISIQLDLTDTRSKIVYKSESAWLPVYIRAGIPNQIPKAAFMAVFILEVDQFILTSLTTSVLDCEEDETPKPLLVFNITKAPLQGYVTHLLDHTRPISSFTWKDLSDMQIAYQPPNSSHSERRHDEVELEVYDFFFERSAPMTVHISIRTADTNAPRVSWNTGLSLLEGQSRAITWEQFQVVDNDDIGAVRLVTVGGLQHGWLTLRGGKGFLFTVADLQAGVVRYHHDDSDSTKDFVVFRIFDGHHSIRHKFPINVLPKDDSPPFLITNVVIELEEGQTILIQGSMLRASDVDASDDYIFFNITKPPQAGEIMKKPGPGLIGYPVHGFLQRDLFNGIIYYRHFGGEIFEDSFQFVLWDSHEPPNLSVPQVATIHITPVDDQLPKEAPGVSRHLVVKETEVAYITKKQLHFIDSESYDRELVYTITTPPFFSFSHRHLDAGKLFMVDSIPKVVKNPTALELRSFTQHAVNYMKVAYMPPMQDIGPHCRDVQFTFSVSNQHGGTLHGICFNITILPVDNQVPEAFTNPLKVTEGGQSIISTEHILISDADTKLDNIDLSLRELPLHGRVELNGFPLNSGGTFSWGDLHTLKVRYQHDGTEVLQDDLLLEVTDGTNSAEFVLHVEVFPVNDEPPVLKADLMPVMNCSEGGEVVITSEYIFATDVDSDNLKLMFVIAREPQHGVVRRAGVTVDQFSQRDVISEAVTYKHTGGEIGLMPCFDTITLVVSDGEAGPFVNGCCYNGPNPSVPLHASFPVYDLNITVYPVDNQPPSIAIGPVFVVDEGCSTALTVNHLSATDPDTAADDLEFVLVSPPQFGYLENILPSVGFEKSNIGISIDSFQWKDMNAFHINYVQSRHLRIEPTADQFTVYVTDGKHHSLEIPFSIIINPTNDEAPDFVVQNITVCEGQMKELDSSIISAVDLDIPQDALLFSITQKPRHGLLIDRGFSKDFSENKQPANPHQKHAPVHSFSMELLKTGMRLTYMHDDSESLADDFTIQLSDGKHKILKTISVEVIPVNDEKPMLSKKAEIAMNMGETRIISSAILSAIDEDSPREKIYYVFERLPQNGQLQLKIGRDWVPLSPGMKCTQEEVDLNLLRYTHTGAMDSQNQDSFTFYLWDGNNRSPALDCQITIKDMEKGDIVILTKPLVVSKGDRGFLTTTTLLAVDGTDKPEELLYVITSPPRYGQIEYVHYPGVPITNFSQMDVVGQTVCYVHKSKVTVSSDRFRFIISNGLRTEHGVFEITLETVDRALPVVTRNKGLRLAQGAVGLLSPDLLQLTDPDTPAENLTFLLVQLPQHGQLYLWGTGLLQHNFTQQDVDSKNVAYRHSGGDSQTDCFTFMATDGTNQGFIVNGRVWEEPVLFTIQVDQLDKTAPRITLLHSPSQVGLLKNGCYGIYITSRVLKASDPDTEDDQIIFKILQGPKHGHLENTTTGEFIHEKFSQKDLNSKTILYIINPSLEVNSDTVEFQIMDPTGNSATPQILELKWSHIEWSQTEYEVCENVGLLPLEIIRRGYSMDSAFVGIKVNQVSAAVGKDFTVIPSKLIQFDPGMSTKMWNIAITYDGLEEDDEVFEVILNSPVNAVLGTKTKAAVKILDSKGGQCHPSYSSNQSKHSTWEKGIWHLLPPGSSSSTTSGSFHLERRPLPSSMQLAVIRGDTLRGFDSTDLSQRKLRTRGNGKTVRPSSVYRNGTDIIYNYHGIVSLKLEDDSFPTHKRKAKVSIISQPQKTIKVAELPQADKVESTTDSHFPRQDQLPSFPKNCTLELKGLFHFEEGIQKLYQCNGIAWKAWSPQTKDVEDKSCPAGWHQHSGYCHILITEQKGTWNAAAQACREQYLGNLVTVFSRQHMRWLWDIGGRKSFWIGLNDQVHAGHWEWIGGEPVAFTNGRRGPSQRSKLGKSCVLVQRQGKWQTKDCRRAKPHNYVCSRKL

Target ORF information:

RefSeq Version	XM_017014316.2
Organism	Homo sapiens(human)
Definition	Homo sapiens FRAS1 related extracellular matrix 1 (FREM1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017014316.2

ORF Insert Sequence:

ATGAACTCTC TGAGTTGGGG GGCTGCGAAT GCCGTGCTGC TGCTGCTCCT CCTGGCCTGG 
GCCAGCCCCA CCTTCATCAG CATCAACCGC GGGGTGAGGG TGATGAAGGG CCACTCTGCC 
TTCCTGTCAG GAGATGACCT GAAGTTTGCC ATCCCTAAAG AGAAAGATGC CTGCAAAGTG 
GAAGTTGTGA TGAATGAGCC AATAACCCAG AGGGTTGGGA AACTCACTCC ACAGGTCTTT 
GACTGCCATT TCCTTCCCAA CGAAGTCAAG TATGTTCACA ATGGTTGTCC AATTCTTGAT 
GAAGACACAG TGAAGCTCAG ACTTTACAGA TTTACTGAAA GAGATACCTT CATAGAAACT 
TTTATCCTGT GGGTCTATCT CCTGGAACCA GACTGTAACA TCATCCATAT GAGTAACAAT 
GTGCTGGAGG TGCCTGAATT CAATGGCTTG TCCCAAGCGA TTGATAAAAA TCTGCTCAGA 
TTCGATTATG ATAGGATGGC TAGCCTGGAA TGTACCGTCA GCCTGGACAC TGCGAGAACT 
CGGCTGCCAG CCCATGGCCA GATGGTTCTC GGGGAGCCTC GACCAGAAGA ACCTCGTGGA 
GATCAGCCAC ACAGTTTTTT CCCAGAGTCC CGTATGATGT CTTTTAATGC CTTTCTGGAA 
CTGAGAGCAA AACTGAAGTG TCCAGGTGGG AGCTGTACCC CAGGATTAAA GAAAATAGGA 
AGCCTCAAAG TGAGCTGTGA GGAGTTCCTG CTGATGGGCC TTCGTTATCA GCATCTGGAT 
CCCCCTTCAC CCAACATTGA TTATATCTCC ATCCAACTGG ACCTCACAGA TACCAGGAGC 
AAAATTGTGT ACAAGTCAGA GAGTGCGTGG CTGCCTGTCT ATATCAGAGC TGGAATTCCG 
AATCAGATTC CAAAGGCTGC ATTCATGGCC GTGTTTATTC TGGAAGTGGA TCAGTTCATC 
CTGACCTCCT TGACTACATC AGTTCTGGAC TGTGAAGAAG ATGAGACCCC TAAACCCTTG 
CTGGTGTTCA ACATTACTAA AGCCCCGCTC CAGGGCTATG TGACTCACCT GTTGGATCAC 
ACCAGACCAA TCTCCTCATT CACCTGGAAA GATCTCAGTG ACATGCAGAT CGCCTATCAG 
CCACCAAACA GCAGCCATTC TGAGAGGAGA CATGATGAGG TAGAATTGGA GGTATACGAC 
TTCTTCTTTG AAAGGAGTGC ACCTATGACA GTCCACATCT CCATCAGAAC AGCAGATACA 
AATGCCCCCC GTGTATCCTG GAATACAGGT CTGAGTCTCC TTGAGGGGCA GTCTCGAGCC 
ATCACTTGGG AACAGTTTCA GGTTGTCGAC AATGACGACA TTGGTGCTGT CCGGCTAGTC 
ACCGTTGGTG GCCTGCAGCA TGGATGGCTG ACTTTAAGAG GGGGGAAAGG GTTTCTCTTC 
ACCGTGGCTG ACCTCCAGGC TGGAGTTGTT CGCTATCATC ATGATGACAG CGACTCCACC 
AAAGACTTCG TGGTCTTCCG GATATTTGAT GGCCATCACA GCATCCGTCA CAAATTCCCC 
ATCAACGTCT TGCCCAAAGA TGATAGTCCC CCGTTCCTCA TAACCAATGT TGTGATTGAA 
CTGGAGGAGG GGCAGACCAT CCTGATCCAG GGATCCATGC TGCGAGCTTC AGATGTGGAC 
GCCAGTGATG ACTACATCTT CTTCAATATC ACAAAGCCTC CACAGGCTGG GGAGATCATG 
AAGAAGCCAG GGCCAGGACT GATAGGCTAT CCTGTCCATG GCTTCCTTCA GAGGGATTTG 
TTTAATGGAA TCATTTATTA TCGTCATTTT GGTGGAGAAA TCTTTGAAGA TTCTTTTCAA 
TTTGTCCTGT GGGACAGCCA TGAACCTCCA AATCTTTCAG TGCCACAGGT GGCAACAATC 
CATATAACTC CAGTGGATGA CCAGCTTCCA AAAGAGGCTC CTGGAGTTTC TCGGCATTTG 
GTTGTCAAGG AAACTGAGGT GGCCTATATA ACTAAGAAAC AGCTACATTT TATAGATTCA 
GAATCATATG ACAGGGAGCT GGTCTACACA ATAACTACTC CTCCATTTTT CTCCTTCAGC 
CACAGACACT TGGATGCTGG GAAATTATTT ATGGTGGACA GCATACCAAA AGTAGTTAAG 
AATCCTACGG CCCTGGAGCT GAGGTCATTC ACTCAGCATG CTGTGAACTA TATGAAAGTG 
GCCTACATGC CCCCCATGCA AGACATTGGT CCCCATTGCA GAGATGTCCA GTTCACATTT 
TCTGTCAGTA ACCAACATGG CGGTACTTTG CATGGGATCT GCTTTAACAT TACAATCCTC 
CCAGTGGACA ATCAAGTTCC TGAGGCGTTC ACCAACCCTC TGAAAGTGAC TGAGGGAGGT 
CAAAGCATCA TCAGCACAGA GCACATTCTA ATTTCTGATG CAGATACCAA GCTGGACAAT 
ATTGACCTCT CCCTGCGGGA ATTGCCTCTG CACGGAAGGG TGGAGCTGAA TGGATTTCCT 
CTAAATTCAG GGGGCACATT TTCTTGGGGC GATCTCCATA CCTTAAAAGT TAGGTATCAA 
CATGATGGAA CTGAAGTTCT TCAGGATGAC CTACTCTTGG AGGTCACCGA TGGCACAAAT 
TCAGCAGAAT TTGTACTACA TGTTGAGGTA TTCCCTGTCA ACGATGAGCC ACCAGTCTTA 
AAGGCTGACC TCATGCCTGT CATGAATTGC TCAGAGGGAG GAGAGGTGGT CATCACCTCT 
GAATACATTT TTGCTACTGA TGTGGACAGC GATAACTTGA AGTTGATGTT TGTGATTGCT 
CGCGAACCTC AGCATGGGGT GGTGAGGAGA GCTGGAGTCA CAGTGGATCA GTTCTCTCAG 
AGAGATGTTA TCTCAGAGGC CGTGACATAC AAACACACAG GTGGCGAGAT TGGCCTGATG 
CCTTGTTTTG ACACCATTAC ATTGGTGGTT TCGGATGGAG AGGCTGGCCC TTTTGTGAAT 
GGCTGTTGCT ACAATGGACC CAATCCATCT GTTCCACTTC ATGCGTCCTT TCCAGTGTAT 
GATCTCAACA TCACGGTATA CCCAGTAGAC AACCAGCCAC CTTCCATTGC AATAGGTCCC 
GTGTTTGTTG TAGATGAGGG CTGCTCAACA GCCCTTACTG TTAACCATTT GTCCGCCACT 
GACCCTGACA CTGCAGCAGA TGACTTGGAA TTTGTTTTGG TTTCTCCTCC TCAGTTTGGC 
TACCTCGAAA ATATACTCCC TTCTGTGGGT TTTGAAAAAA GCAATATTGG CATAAGTATA 
GATTCATTTC AGTGGAAAGA CATGAACGCT TTTCACATTA ACTATGTGCA GTCCAGGCAT 
CTGAGGATAG AACCAACTGC CGACCAGTTC ACGGTGTACG TCACAGATGG GAAGCATCAC 
TCCTTGGAGA TACCATTTTC TATTATAATC AACCCCACAA ATGATGAAGC TCCTGACTTT 
GTAGTGCAGA ATATTACTGT GTGTGAGGGT CAGATGAAAG AGCTGGACTC TTCCATCATC 
AGCGCTGTGG ACCTGGACAT TCCCCAGGAT GCCCTGCTGT TCAGCATCAC TCAAAAGCCA 
CGCCATGGCC TCCTCATCGA TAGGGGGTTT AGCAAAGACT TCTCTGAGAA TAAGCAGCCA 
GCCAACCCTC ACCAGAAACA TGCACCTGTT CACAGCTTTT CCATGGAACT CCTCAAGACT 
GGAATGAGGT TGACGTACAT GCATGATGAC TCAGAGAGCC TTGCTGATGA TTTTACAATC 
CAATTGTCAG ATGGGAAACA TAAGATACTT AAAACCATTT CAGTAGAGGT CATCCCAGTT 
AATGATGAAA AACCAATGCT GAGCAAGAAG GCTGAAATTG CAATGAATAT GGGTGAAACT 
CGTATTATTT CCAGTGCTAT TCTTTCAGCC ATAGATGAAG ACTCACCCAG GGAGAAGATT 
TACTATGTAT TTGAAAGGCT TCCCCAAAAT GGGCAACTTC AGCTTAAGAT AGGGAGGGAC 
TGGGTTCCTC TCTCCCCTGG CATGAAATGC ACTCAGGAGG AAGTGGATCT GAACTTGCTG 
AGATACACAC ACACCGGGGC AATGGATTCC CAGAATCAAG ATAGCTTCAC CTTCTACCTT 
TGGGATGGCA ACAACAGGTC CCCTGCTCTT GACTGTCAAA TCACCATCAA GGACATGGAA 
AAAGGTGATA TTGTCATCCT TACAAAACCA CTCGTGGTGT CTAAAGGTGA TAGAGGTTTC 
TTAACAACCA CCACGCTCCT GGCTGTGGAC GGAACAGACA AGCCTGAGGA ACTGCTCTAT 
GTCATCACCT CCCCTCCGCG ATATGGCCAG ATCGAATATG TTCACTATCC TGGAGTTCCC 
ATTACAAACT TCAGCCAAAT GGATGTAGTG GGGCAGACAG TTTGCTATGT ACACAAGAGC 
AAGGTGACTG TCTCCAGTGA CAGATTCAGA TTCATCATCA GCAATGGACT GCGGACCGAG 
CACGGGGTGT TTGAGATCAC ACTGGAGACT GTGGACAGAG CCCTGCCTGT GGTAACCAGG 
AACAAGGGGT TGAGACTGGC CCAAGGGGCC GTGGGCCTGC TTTCCCCTGA CCTCCTTCAG 
CTGACCGACC CTGATACACC TGCGGAGAAC CTCACCTTCC TCTTGGTTCA GCTCCCCCAG 
CATGGCCAGC TCTACCTGTG GGGGACAGGG CTACTTCAAC ACAATTTCAC CCAGCAGGAT 
GTGGACAGCA AGAATGTGGC CTATCGGCAC TCAGGAGGGG ACTCCCAGAC TGACTGCTTT 
ACTTTCATGG CCACAGATGG GACAAACCAA GGCTTTATTG TGAATGGGAG AGTGTGGGAA 
GAACCTGTTT TATTCACCAT TCAGGTAGAC CAATTGGACA AAACAGCTCC TCGTATCACA 
CTCTTGCATT CCCCTTCTCA AGTGGGGCTC CTGAAAAATG GCTGCTACGG GATTTACATC 
ACTTCCCGCG TGTTGAAGGC ATCAGACCCT GACACTGAGG ACGATCAGAT CATCTTTAAA 
ATTCTACAAG GCCCAAAACA TGGACATCTG GAGAACACAA CAACAGGTGA ATTTATCCAT 
GAGAAATTTA GCCAAAAGGA CTTAAACAGT AAGACTATTC TTTACATCAT AAACCCATCT 
TTGGAAGTAA ATTCAGATAC CGTGGAATTT CAAATCATGG ACCCCACAGG GAACTCGGCC 
ACTCCTCAAA TTTTGGAACT GAAGTGGTCT CATATTGAAT GGTCACAGAC CGAATATGAA 
GTCTGTGAGA ATGTGGGTTT GTTGCCCTTG GAAATTATCA GAAGGGGATA TTCCATGGAC 
TCGGCCTTTG TGGGTATAAA GGTCAACCAA GTGTCAGCTG CAGTTGGAAA AGATTTCACC 
GTGATTCCAT CTAAACTGAT TCAGTTTGAC CCAGGAATGT CAACTAAGAT GTGGAATATA 
GCAATTACCT ATGACGGATT AGAGGAAGAT GATGAGGTCT TTGAAGTAAT TCTGAACTCC 
CCTGTGAATG CAGTTCTTGG CACAAAGACA AAAGCTGCAG TGAAAATTTT GGACTCAAAA 
GGAGGACAAT GCCATCCTTC ATATTCCTCC AACCAAAGCA AGCACAGCAC ATGGGAGAAG 
GGCATTTGGC ATCTGCTGCC CCCAGGGTCT TCCTCATCCA CCACTTCTGG TTCCTTTCAT 
CTGGAAAGAA GACCTCTTCC ATCTTCCATG CAGCTAGCAG TCATCAGGGG AGACACCCTG 
CGGGGCTTTG ATTCTACAGA TCTTTCTCAA AGGAAGCTTA GGACCCGTGG GAATGGCAAA 
ACAGTTCGTC CATCCTCTGT TTATAGAAAT GGAACAGACA TCATCTATAA TTATCATGGG 
ATAGTTTCCT TGAAACTGGA GGATGACAGT TTCCCAACTC ACAAAAGGAA GGCCAAAGTA 
TCCATCATTA GTCAGCCACA AAAGACAATC AAAGTGGCAG AACTGCCTCA AGCAGATAAG 
GTGGAATCCA CAACTGACTC ACACTTCCCC AGACAGGACC AGTTGCCCTC ATTTCCAAAG 
AACTGCACTC TGGAATTAAA GGGACTCTTC CATTTTGAAG AAGGCATCCA GAAGCTGTAT 
CAGTGCAATG GGATCGCCTG GAAAGCCTGG AGTCCCCAAA CCAAGGATGT GGAAGACAAA 
TCCTGTCCAG CCGGGTGGCA CCAGCACTCA GGCTACTGTC ACATCTTGAT CACAGAGCAG 
AAAGGCACCT GGAATGCGGC TGCCCAAGCT TGCAGGGAAC AATACCTGGG CAACCTTGTA 
ACTGTATTCT CCAGGCAGCA CATGCGGTGG CTCTGGGACA TTGGTGGGAG AAAGTCCTTT 
TGGATAGGTT TGAACGACCA AGTGCATGCT GGCCACTGGG AGTGGATCGG TGGTGAACCT 
GTTGCCTTCA CCAATGGGAG AAGAGGGCCC TCTCAACGCT CCAAGCTTGG AAAGAGCTGT 
GTTTTGGTTC AAAGACAAGG GAAATGGCAA ACAAAAGACT GTAGGAGAGC CAAACCTCAT 
AATTATGTGT GTTCCAGAAA ACTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FREM1 ( XM_017014317.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016869806.1 Homo sapiens FRAS1 related extracellular matrix 1 (FREM1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu98459
Clone ID Related Accession (Same CDS sequence)	XM_017014320.1 , XM_017014318.1 , XM_017014316.1 , XM_017014316.2 , XM_017014317.1 , XM_017014319.2 , XM_017014321.2 , XM_017014323.1 , XM_017014322.1 , XM_017014321.1 , XM_017014320.2 , XM_017014319.1
Accession Version	XM_017014317.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6567bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	FRAS1-related extracellular matrix protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACTCTC TGAGTTGGGG GGCTGCGAAT GCCGTGCTGC TGCTGCTCCT CCTGGCCTGG 
GCCAGCCCCA CCTTCATCAG CATCAACCGC GGGGTGAGGG TGATGAAGGG CCACTCTGCC 
TTCCTGTCAG GAGATGACCT GAAGTTTGCC ATCCCTAAAG AGAAAGATGC CTGCAAAGTG 
GAAGTTGTGA TGAATGAGCC AATAACCCAG AGGGTTGGGA AACTCACTCC ACAGGTCTTT 
GACTGCCATT TCCTTCCCAA CGAAGTCAAG TATGTTCACA ATGGTTGTCC AATTCTTGAT 
GAAGACACAG TGAAGCTCAG ACTTTACAGA TTTACTGAAA GAGATACCTT CATAGAAACT 
TTTATCCTGT GGGTCTATCT CCTGGAACCA GACTGTAACA TCATCCATAT GAGTAACAAT 
GTGCTGGAGG TGCCTGAATT CAATGGCTTG TCCCAAGCGA TTGATAAAAA TCTGCTCAGA 
TTCGATTATG ATAGGATGGC TAGCCTGGAA TGTACCGTCA GCCTGGACAC TGCGAGAACT 
CGGCTGCCAG CCCATGGCCA GATGGTTCTC GGGGAGCCTC GACCAGAAGA ACCTCGTGGA 
GATCAGCCAC ACAGTTTTTT CCCAGAGTCC CGTATGATGT CTTTTAATGC CTTTCTGGAA 
CTGAGAGCAA AACTGAAGTG TCCAGGTGGG AGCTGTACCC CAGGATTAAA GAAAATAGGA 
AGCCTCAAAG TGAGCTGTGA GGAGTTCCTG CTGATGGGCC TTCGTTATCA GCATCTGGAT 
CCCCCTTCAC CCAACATTGA TTATATCTCC ATCCAACTGG ACCTCACAGA TACCAGGAGC 
AAAATTGTGT ACAAGTCAGA GAGTGCGTGG CTGCCTGTCT ATATCAGAGC TGGAATTCCG 
AATCAGATTC CAAAGGCTGC ATTCATGGCC GTGTTTATTC TGGAAGTGGA TCAGTTCATC 
CTGACCTCCT TGACTACATC AGTTCTGGAC TGTGAAGAAG ATGAGACCCC TAAACCCTTG 
CTGGTGTTCA ACATTACTAA AGCCCCGCTC CAGGGCTATG TGACTCACCT GTTGGATCAC 
ACCAGACCAA TCTCCTCATT CACCTGGAAA GATCTCAGTG ACATGCAGAT CGCCTATCAG 
CCACCAAACA GCAGCCATTC TGAGAGGAGA CATGATGAGG TAGAATTGGA GGTATACGAC 
TTCTTCTTTG AAAGGAGTGC ACCTATGACA GTCCACATCT CCATCAGAAC AGCAGATACA 
AATGCCCCCC GTGTATCCTG GAATACAGGT CTGAGTCTCC TTGAGGGGCA GTCTCGAGCC 
ATCACTTGGG AACAGTTTCA GGTTGTCGAC AATGACGACA TTGGTGCTGT CCGGCTAGTC 
ACCGTTGGTG GCCTGCAGCA TGGATGGCTG ACTTTAAGAG GGGGGAAAGG GTTTCTCTTC 
ACCGTGGCTG ACCTCCAGGC TGGAGTTGTT CGCTATCATC ATGATGACAG CGACTCCACC 
AAAGACTTCG TGGTCTTCCG GATATTTGAT GGCCATCACA GCATCCGTCA CAAATTCCCC 
ATCAACGTCT TGCCCAAAGA TGATAGTCCC CCGTTCCTCA TAACCAATGT TGTGATTGAA 
CTGGAGGAGG GGCAGACCAT CCTGATCCAG GGATCCATGC TGCGAGCTTC AGATGTGGAC 
GCCAGTGATG ACTACATCTT CTTCAATATC ACAAAGCCTC CACAGGCTGG GGAGATCATG 
AAGAAGCCAG GGCCAGGACT GATAGGCTAT CCTGTCCATG GCTTCCTTCA GAGGGATTTG 
TTTAATGGAA TCATTTATTA TCGTCATTTT GGTGGAGAAA TCTTTGAAGA TTCTTTTCAA 
TTTGTCCTGT GGGACAGCCA TGAACCTCCA AATCTTTCAG TGCCACAGGT GGCAACAATC 
CATATAACTC CAGTGGATGA CCAGCTTCCA AAAGAGGCTC CTGGAGTTTC TCGGCATTTG 
GTTGTCAAGG AAACTGAGGT GGCCTATATA ACTAAGAAAC AGCTACATTT TATAGATTCA 
GAATCATATG ACAGGGAGCT GGTCTACACA ATAACTACTC CTCCATTTTT CTCCTTCAGC 
CACAGACACT TGGATGCTGG GAAATTATTT ATGGTGGACA GCATACCAAA AGTAGTTAAG 
AATCCTACGG CCCTGGAGCT GAGGTCATTC ACTCAGCATG CTGTGAACTA TATGAAAGTG 
GCCTACATGC CCCCCATGCA AGACATTGGT CCCCATTGCA GAGATGTCCA GTTCACATTT 
TCTGTCAGTA ACCAACATGG CGGTACTTTG CATGGGATCT GCTTTAACAT TACAATCCTC 
CCAGTGGACA ATCAAGTTCC TGAGGCGTTC ACCAACCCTC TGAAAGTGAC TGAGGGAGGT 
CAAAGCATCA TCAGCACAGA GCACATTCTA ATTTCTGATG CAGATACCAA GCTGGACAAT 
ATTGACCTCT CCCTGCGGGA ATTGCCTCTG CACGGAAGGG TGGAGCTGAA TGGATTTCCT 
CTAAATTCAG GGGGCACATT TTCTTGGGGC GATCTCCATA CCTTAAAAGT TAGGTATCAA 
CATGATGGAA CTGAAGTTCT TCAGGATGAC CTACTCTTGG AGGTCACCGA TGGCACAAAT 
TCAGCAGAAT TTGTACTACA TGTTGAGGTA TTCCCTGTCA ACGATGAGCC ACCAGTCTTA 
AAGGCTGACC TCATGCCTGT CATGAATTGC TCAGAGGGAG GAGAGGTGGT CATCACCTCT 
GAATACATTT TTGCTACTGA TGTGGACAGC GATAACTTGA AGTTGATGTT TGTGATTGCT 
CGCGAACCTC AGCATGGGGT GGTGAGGAGA GCTGGAGTCA CAGTGGATCA GTTCTCTCAG 
AGAGATGTTA TCTCAGAGGC CGTGACATAC AAACACACAG GTGGCGAGAT TGGCCTGATG 
CCTTGTTTTG ACACCATTAC ATTGGTGGTT TCGGATGGAG AGGCTGGCCC TTTTGTGAAT 
GGCTGTTGCT ACAATGGACC CAATCCATCT GTTCCACTTC ATGCGTCCTT TCCAGTGTAT 
GATCTCAACA TCACGGTATA CCCAGTAGAC AACCAGCCAC CTTCCATTGC AATAGGTCCC 
GTGTTTGTTG TAGATGAGGG CTGCTCAACA GCCCTTACTG TTAACCATTT GTCCGCCACT 
GACCCTGACA CTGCAGCAGA TGACTTGGAA TTTGTTTTGG TTTCTCCTCC TCAGTTTGGC 
TACCTCGAAA ATATACTCCC TTCTGTGGGT TTTGAAAAAA GCAATATTGG CATAAGTATA 
GATTCATTTC AGTGGAAAGA CATGAACGCT TTTCACATTA ACTATGTGCA GTCCAGGCAT 
CTGAGGATAG AACCAACTGC CGACCAGTTC ACGGTGTACG TCACAGATGG GAAGCATCAC 
TCCTTGGAGA TACCATTTTC TATTATAATC AACCCCACAA ATGATGAAGC TCCTGACTTT 
GTAGTGCAGA ATATTACTGT GTGTGAGGGT CAGATGAAAG AGCTGGACTC TTCCATCATC 
AGCGCTGTGG ACCTGGACAT TCCCCAGGAT GCCCTGCTGT TCAGCATCAC TCAAAAGCCA 
CGCCATGGCC TCCTCATCGA TAGGGGGTTT AGCAAAGACT TCTCTGAGAA TAAGCAGCCA 
GCCAACCCTC ACCAGAAACA TGCACCTGTT CACAGCTTTT CCATGGAACT CCTCAAGACT 
GGAATGAGGT TGACGTACAT GCATGATGAC TCAGAGAGCC TTGCTGATGA TTTTACAATC 
CAATTGTCAG ATGGGAAACA TAAGATACTT AAAACCATTT CAGTAGAGGT CATCCCAGTT 
AATGATGAAA AACCAATGCT GAGCAAGAAG GCTGAAATTG CAATGAATAT GGGTGAAACT 
CGTATTATTT CCAGTGCTAT TCTTTCAGCC ATAGATGAAG ACTCACCCAG GGAGAAGATT 
TACTATGTAT TTGAAAGGCT TCCCCAAAAT GGGCAACTTC AGCTTAAGAT AGGGAGGGAC 
TGGGTTCCTC TCTCCCCTGG CATGAAATGC ACTCAGGAGG AAGTGGATCT GAACTTGCTG 
AGATACACAC ACACCGGGGC AATGGATTCC CAGAATCAAG ATAGCTTCAC CTTCTACCTT 
TGGGATGGCA ACAACAGGTC CCCTGCTCTT GACTGTCAAA TCACCATCAA GGACATGGAA 
AAAGGTGATA TTGTCATCCT TACAAAACCA CTCGTGGTGT CTAAAGGTGA TAGAGGTTTC 
TTAACAACCA CCACGCTCCT GGCTGTGGAC GGAACAGACA AGCCTGAGGA ACTGCTCTAT 
GTCATCACCT CCCCTCCGCG ATATGGCCAG ATCGAATATG TTCACTATCC TGGAGTTCCC 
ATTACAAACT TCAGCCAAAT GGATGTAGTG GGGCAGACAG TTTGCTATGT ACACAAGAGC 
AAGGTGACTG TCTCCAGTGA CAGATTCAGA TTCATCATCA GCAATGGACT GCGGACCGAG 
CACGGGGTGT TTGAGATCAC ACTGGAGACT GTGGACAGAG CCCTGCCTGT GGTAACCAGG 
AACAAGGGGT TGAGACTGGC CCAAGGGGCC GTGGGCCTGC TTTCCCCTGA CCTCCTTCAG 
CTGACCGACC CTGATACACC TGCGGAGAAC CTCACCTTCC TCTTGGTTCA GCTCCCCCAG 
CATGGCCAGC TCTACCTGTG GGGGACAGGG CTACTTCAAC ACAATTTCAC CCAGCAGGAT 
GTGGACAGCA AGAATGTGGC CTATCGGCAC TCAGGAGGGG ACTCCCAGAC TGACTGCTTT 
ACTTTCATGG CCACAGATGG GACAAACCAA GGCTTTATTG TGAATGGGAG AGTGTGGGAA 
GAACCTGTTT TATTCACCAT TCAGGTAGAC CAATTGGACA AAACAGCTCC TCGTATCACA 
CTCTTGCATT CCCCTTCTCA AGTGGGGCTC CTGAAAAATG GCTGCTACGG GATTTACATC 
ACTTCCCGCG TGTTGAAGGC ATCAGACCCT GACACTGAGG ACGATCAGAT CATCTTTAAA 
ATTCTACAAG GCCCAAAACA TGGACATCTG GAGAACACAA CAACAGGTGA ATTTATCCAT 
GAGAAATTTA GCCAAAAGGA CTTAAACAGT AAGACTATTC TTTACATCAT AAACCCATCT 
TTGGAAGTAA ATTCAGATAC CGTGGAATTT CAAATCATGG ACCCCACAGG GAACTCGGCC 
ACTCCTCAAA TTTTGGAACT GAAGTGGTCT CATATTGAAT GGTCACAGAC CGAATATGAA 
GTCTGTGAGA ATGTGGGTTT GTTGCCCTTG GAAATTATCA GAAGGGGATA TTCCATGGAC 
TCGGCCTTTG TGGGTATAAA GGTCAACCAA GTGTCAGCTG CAGTTGGAAA AGATTTCACC 
GTGATTCCAT CTAAACTGAT TCAGTTTGAC CCAGGAATGT CAACTAAGAT GTGGAATATA 
GCAATTACCT ATGACGGATT AGAGGAAGAT GATGAGGTCT TTGAAGTAAT TCTGAACTCC 
CCTGTGAATG CAGTTCTTGG CACAAAGACA AAAGCTGCAG TGAAAATTTT GGACTCAAAA 
GGAGGACAAT GCCATCCTTC ATATTCCTCC AACCAAAGCA AGCACAGCAC ATGGGAGAAG 
GGCATTTGGC ATCTGCTGCC CCCAGGGTCT TCCTCATCCA CCACTTCTGG TTCCTTTCAT 
CTGGAAAGAA GACCTCTTCC ATCTTCCATG CAGCTAGCAG TCATCAGGGG AGACACCCTG 
CGGGGCTTTG ATTCTACAGA TCTTTCTCAA AGGAAGCTTA GGACCCGTGG GAATGGCAAA 
ACAGTTCGTC CATCCTCTGT TTATAGAAAT GGAACAGACA TCATCTATAA TTATCATGGG 
ATAGTTTCCT TGAAACTGGA GGATGACAGT TTCCCAACTC ACAAAAGGAA GGCCAAAGTA 
TCCATCATTA GTCAGCCACA AAAGACAATC AAAGTGGCAG AACTGCCTCA AGCAGATAAG 
GTGGAATCCA CAACTGACTC ACACTTCCCC AGACAGGACC AGTTGCCCTC ATTTCCAAAG 
AACTGCACTC TGGAATTAAA GGGACTCTTC CATTTTGAAG AAGGCATCCA GAAGCTGTAT 
CAGTGCAATG GGATCGCCTG GAAAGCCTGG AGTCCCCAAA CCAAGGATGT GGAAGACAAA 
TCCTGTCCAG CCGGGTGGCA CCAGCACTCA GGCTACTGTC ACATCTTGAT CACAGAGCAG 
AAAGGCACCT GGAATGCGGC TGCCCAAGCT TGCAGGGAAC AATACCTGGG CAACCTTGTA 
ACTGTATTCT CCAGGCAGCA CATGCGGTGG CTCTGGGACA TTGGTGGGAG AAAGTCCTTT 
TGGATAGGTT TGAACGACCA AGTGCATGCT GGCCACTGGG AGTGGATCGG TGGTGAACCT 
GTTGCCTTCA CCAATGGGAG AAGAGGGCCC TCTCAACGCT CCAAGCTTGG AAAGAGCTGT 
GTTTTGGTTC AAAGACAAGG GAAATGGCAA ACAAAAGACT GTAGGAGAGC CAAACCTCAT 
AATTATGTGT GTTCCAGAAA ACTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016869806.1
CDS	608..7174
Translation	MNSLSWGAANAVLLLLLLAWASPTFISINRGVRVMKGHSAFLSGDDLKFAIPKEKDACKVEVVMNEPITQRVGKLTPQVFDCHFLPNEVKYVHNGCPILDEDTVKLRLYRFTERDTFIETFILWVYLLEPDCNIIHMSNNVLEVPEFNGLSQAIDKNLLRFDYDRMASLECTVSLDTARTRLPAHGQMVLGEPRPEEPRGDQPHSFFPESRMMSFNAFLELRAKLKCPGGSCTPGLKKIGSLKVSCEEFLLMGLRYQHLDPPSPNIDYISIQLDLTDTRSKIVYKSESAWLPVYIRAGIPNQIPKAAFMAVFILEVDQFILTSLTTSVLDCEEDETPKPLLVFNITKAPLQGYVTHLLDHTRPISSFTWKDLSDMQIAYQPPNSSHSERRHDEVELEVYDFFFERSAPMTVHISIRTADTNAPRVSWNTGLSLLEGQSRAITWEQFQVVDNDDIGAVRLVTVGGLQHGWLTLRGGKGFLFTVADLQAGVVRYHHDDSDSTKDFVVFRIFDGHHSIRHKFPINVLPKDDSPPFLITNVVIELEEGQTILIQGSMLRASDVDASDDYIFFNITKPPQAGEIMKKPGPGLIGYPVHGFLQRDLFNGIIYYRHFGGEIFEDSFQFVLWDSHEPPNLSVPQVATIHITPVDDQLPKEAPGVSRHLVVKETEVAYITKKQLHFIDSESYDRELVYTITTPPFFSFSHRHLDAGKLFMVDSIPKVVKNPTALELRSFTQHAVNYMKVAYMPPMQDIGPHCRDVQFTFSVSNQHGGTLHGICFNITILPVDNQVPEAFTNPLKVTEGGQSIISTEHILISDADTKLDNIDLSLRELPLHGRVELNGFPLNSGGTFSWGDLHTLKVRYQHDGTEVLQDDLLLEVTDGTNSAEFVLHVEVFPVNDEPPVLKADLMPVMNCSEGGEVVITSEYIFATDVDSDNLKLMFVIAREPQHGVVRRAGVTVDQFSQRDVISEAVTYKHTGGEIGLMPCFDTITLVVSDGEAGPFVNGCCYNGPNPSVPLHASFPVYDLNITVYPVDNQPPSIAIGPVFVVDEGCSTALTVNHLSATDPDTAADDLEFVLVSPPQFGYLENILPSVGFEKSNIGISIDSFQWKDMNAFHINYVQSRHLRIEPTADQFTVYVTDGKHHSLEIPFSIIINPTNDEAPDFVVQNITVCEGQMKELDSSIISAVDLDIPQDALLFSITQKPRHGLLIDRGFSKDFSENKQPANPHQKHAPVHSFSMELLKTGMRLTYMHDDSESLADDFTIQLSDGKHKILKTISVEVIPVNDEKPMLSKKAEIAMNMGETRIISSAILSAIDEDSPREKIYYVFERLPQNGQLQLKIGRDWVPLSPGMKCTQEEVDLNLLRYTHTGAMDSQNQDSFTFYLWDGNNRSPALDCQITIKDMEKGDIVILTKPLVVSKGDRGFLTTTTLLAVDGTDKPEELLYVITSPPRYGQIEYVHYPGVPITNFSQMDVVGQTVCYVHKSKVTVSSDRFRFIISNGLRTEHGVFEITLETVDRALPVVTRNKGLRLAQGAVGLLSPDLLQLTDPDTPAENLTFLLVQLPQHGQLYLWGTGLLQHNFTQQDVDSKNVAYRHSGGDSQTDCFTFMATDGTNQGFIVNGRVWEEPVLFTIQVDQLDKTAPRITLLHSPSQVGLLKNGCYGIYITSRVLKASDPDTEDDQIIFKILQGPKHGHLENTTTGEFIHEKFSQKDLNSKTILYIINPSLEVNSDTVEFQIMDPTGNSATPQILELKWSHIEWSQTEYEVCENVGLLPLEIIRRGYSMDSAFVGIKVNQVSAAVGKDFTVIPSKLIQFDPGMSTKMWNIAITYDGLEEDDEVFEVILNSPVNAVLGTKTKAAVKILDSKGGQCHPSYSSNQSKHSTWEKGIWHLLPPGSSSSTTSGSFHLERRPLPSSMQLAVIRGDTLRGFDSTDLSQRKLRTRGNGKTVRPSSVYRNGTDIIYNYHGIVSLKLEDDSFPTHKRKAKVSIISQPQKTIKVAELPQADKVESTTDSHFPRQDQLPSFPKNCTLELKGLFHFEEGIQKLYQCNGIAWKAWSPQTKDVEDKSCPAGWHQHSGYCHILITEQKGTWNAAAQACREQYLGNLVTVFSRQHMRWLWDIGGRKSFWIGLNDQVHAGHWEWIGGEPVAFTNGRRGPSQRSKLGKSCVLVQRQGKWQTKDCRRAKPHNYVCSRKL

Target ORF information:

RefSeq Version	XM_017014317.1
Organism	Homo sapiens(human)
Definition	Homo sapiens FRAS1 related extracellular matrix 1 (FREM1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017014317.1

ORF Insert Sequence:

ATGAACTCTC TGAGTTGGGG GGCTGCGAAT GCCGTGCTGC TGCTGCTCCT CCTGGCCTGG 
GCCAGCCCCA CCTTCATCAG CATCAACCGC GGGGTGAGGG TGATGAAGGG CCACTCTGCC 
TTCCTGTCAG GAGATGACCT GAAGTTTGCC ATCCCTAAAG AGAAAGATGC CTGCAAAGTG 
GAAGTTGTGA TGAATGAGCC AATAACCCAG AGGGTTGGGA AACTCACTCC ACAGGTCTTT 
GACTGCCATT TCCTTCCCAA CGAAGTCAAG TATGTTCACA ATGGTTGTCC AATTCTTGAT 
GAAGACACAG TGAAGCTCAG ACTTTACAGA TTTACTGAAA GAGATACCTT CATAGAAACT 
TTTATCCTGT GGGTCTATCT CCTGGAACCA GACTGTAACA TCATCCATAT GAGTAACAAT 
GTGCTGGAGG TGCCTGAATT CAATGGCTTG TCCCAAGCGA TTGATAAAAA TCTGCTCAGA 
TTCGATTATG ATAGGATGGC TAGCCTGGAA TGTACCGTCA GCCTGGACAC TGCGAGAACT 
CGGCTGCCAG CCCATGGCCA GATGGTTCTC GGGGAGCCTC GACCAGAAGA ACCTCGTGGA 
GATCAGCCAC ACAGTTTTTT CCCAGAGTCC CGTATGATGT CTTTTAATGC CTTTCTGGAA 
CTGAGAGCAA AACTGAAGTG TCCAGGTGGG AGCTGTACCC CAGGATTAAA GAAAATAGGA 
AGCCTCAAAG TGAGCTGTGA GGAGTTCCTG CTGATGGGCC TTCGTTATCA GCATCTGGAT 
CCCCCTTCAC CCAACATTGA TTATATCTCC ATCCAACTGG ACCTCACAGA TACCAGGAGC 
AAAATTGTGT ACAAGTCAGA GAGTGCGTGG CTGCCTGTCT ATATCAGAGC TGGAATTCCG 
AATCAGATTC CAAAGGCTGC ATTCATGGCC GTGTTTATTC TGGAAGTGGA TCAGTTCATC 
CTGACCTCCT TGACTACATC AGTTCTGGAC TGTGAAGAAG ATGAGACCCC TAAACCCTTG 
CTGGTGTTCA ACATTACTAA AGCCCCGCTC CAGGGCTATG TGACTCACCT GTTGGATCAC 
ACCAGACCAA TCTCCTCATT CACCTGGAAA GATCTCAGTG ACATGCAGAT CGCCTATCAG 
CCACCAAACA GCAGCCATTC TGAGAGGAGA CATGATGAGG TAGAATTGGA GGTATACGAC 
TTCTTCTTTG AAAGGAGTGC ACCTATGACA GTCCACATCT CCATCAGAAC AGCAGATACA 
AATGCCCCCC GTGTATCCTG GAATACAGGT CTGAGTCTCC TTGAGGGGCA GTCTCGAGCC 
ATCACTTGGG AACAGTTTCA GGTTGTCGAC AATGACGACA TTGGTGCTGT CCGGCTAGTC 
ACCGTTGGTG GCCTGCAGCA TGGATGGCTG ACTTTAAGAG GGGGGAAAGG GTTTCTCTTC 
ACCGTGGCTG ACCTCCAGGC TGGAGTTGTT CGCTATCATC ATGATGACAG CGACTCCACC 
AAAGACTTCG TGGTCTTCCG GATATTTGAT GGCCATCACA GCATCCGTCA CAAATTCCCC 
ATCAACGTCT TGCCCAAAGA TGATAGTCCC CCGTTCCTCA TAACCAATGT TGTGATTGAA 
CTGGAGGAGG GGCAGACCAT CCTGATCCAG GGATCCATGC TGCGAGCTTC AGATGTGGAC 
GCCAGTGATG ACTACATCTT CTTCAATATC ACAAAGCCTC CACAGGCTGG GGAGATCATG 
AAGAAGCCAG GGCCAGGACT GATAGGCTAT CCTGTCCATG GCTTCCTTCA GAGGGATTTG 
TTTAATGGAA TCATTTATTA TCGTCATTTT GGTGGAGAAA TCTTTGAAGA TTCTTTTCAA 
TTTGTCCTGT GGGACAGCCA TGAACCTCCA AATCTTTCAG TGCCACAGGT GGCAACAATC 
CATATAACTC CAGTGGATGA CCAGCTTCCA AAAGAGGCTC CTGGAGTTTC TCGGCATTTG 
GTTGTCAAGG AAACTGAGGT GGCCTATATA ACTAAGAAAC AGCTACATTT TATAGATTCA 
GAATCATATG ACAGGGAGCT GGTCTACACA ATAACTACTC CTCCATTTTT CTCCTTCAGC 
CACAGACACT TGGATGCTGG GAAATTATTT ATGGTGGACA GCATACCAAA AGTAGTTAAG 
AATCCTACGG CCCTGGAGCT GAGGTCATTC ACTCAGCATG CTGTGAACTA TATGAAAGTG 
GCCTACATGC CCCCCATGCA AGACATTGGT CCCCATTGCA GAGATGTCCA GTTCACATTT 
TCTGTCAGTA ACCAACATGG CGGTACTTTG CATGGGATCT GCTTTAACAT TACAATCCTC 
CCAGTGGACA ATCAAGTTCC TGAGGCGTTC ACCAACCCTC TGAAAGTGAC TGAGGGAGGT 
CAAAGCATCA TCAGCACAGA GCACATTCTA ATTTCTGATG CAGATACCAA GCTGGACAAT 
ATTGACCTCT CCCTGCGGGA ATTGCCTCTG CACGGAAGGG TGGAGCTGAA TGGATTTCCT 
CTAAATTCAG GGGGCACATT TTCTTGGGGC GATCTCCATA CCTTAAAAGT TAGGTATCAA 
CATGATGGAA CTGAAGTTCT TCAGGATGAC CTACTCTTGG AGGTCACCGA TGGCACAAAT 
TCAGCAGAAT TTGTACTACA TGTTGAGGTA TTCCCTGTCA ACGATGAGCC ACCAGTCTTA 
AAGGCTGACC TCATGCCTGT CATGAATTGC TCAGAGGGAG GAGAGGTGGT CATCACCTCT 
GAATACATTT TTGCTACTGA TGTGGACAGC GATAACTTGA AGTTGATGTT TGTGATTGCT 
CGCGAACCTC AGCATGGGGT GGTGAGGAGA GCTGGAGTCA CAGTGGATCA GTTCTCTCAG 
AGAGATGTTA TCTCAGAGGC CGTGACATAC AAACACACAG GTGGCGAGAT TGGCCTGATG 
CCTTGTTTTG ACACCATTAC ATTGGTGGTT TCGGATGGAG AGGCTGGCCC TTTTGTGAAT 
GGCTGTTGCT ACAATGGACC CAATCCATCT GTTCCACTTC ATGCGTCCTT TCCAGTGTAT 
GATCTCAACA TCACGGTATA CCCAGTAGAC AACCAGCCAC CTTCCATTGC AATAGGTCCC 
GTGTTTGTTG TAGATGAGGG CTGCTCAACA GCCCTTACTG TTAACCATTT GTCCGCCACT 
GACCCTGACA CTGCAGCAGA TGACTTGGAA TTTGTTTTGG TTTCTCCTCC TCAGTTTGGC 
TACCTCGAAA ATATACTCCC TTCTGTGGGT TTTGAAAAAA GCAATATTGG CATAAGTATA 
GATTCATTTC AGTGGAAAGA CATGAACGCT TTTCACATTA ACTATGTGCA GTCCAGGCAT 
CTGAGGATAG AACCAACTGC CGACCAGTTC ACGGTGTACG TCACAGATGG GAAGCATCAC 
TCCTTGGAGA TACCATTTTC TATTATAATC AACCCCACAA ATGATGAAGC TCCTGACTTT 
GTAGTGCAGA ATATTACTGT GTGTGAGGGT CAGATGAAAG AGCTGGACTC TTCCATCATC 
AGCGCTGTGG ACCTGGACAT TCCCCAGGAT GCCCTGCTGT TCAGCATCAC TCAAAAGCCA 
CGCCATGGCC TCCTCATCGA TAGGGGGTTT AGCAAAGACT TCTCTGAGAA TAAGCAGCCA 
GCCAACCCTC ACCAGAAACA TGCACCTGTT CACAGCTTTT CCATGGAACT CCTCAAGACT 
GGAATGAGGT TGACGTACAT GCATGATGAC TCAGAGAGCC TTGCTGATGA TTTTACAATC 
CAATTGTCAG ATGGGAAACA TAAGATACTT AAAACCATTT CAGTAGAGGT CATCCCAGTT 
AATGATGAAA AACCAATGCT GAGCAAGAAG GCTGAAATTG CAATGAATAT GGGTGAAACT 
CGTATTATTT CCAGTGCTAT TCTTTCAGCC ATAGATGAAG ACTCACCCAG GGAGAAGATT 
TACTATGTAT TTGAAAGGCT TCCCCAAAAT GGGCAACTTC AGCTTAAGAT AGGGAGGGAC 
TGGGTTCCTC TCTCCCCTGG CATGAAATGC ACTCAGGAGG AAGTGGATCT GAACTTGCTG 
AGATACACAC ACACCGGGGC AATGGATTCC CAGAATCAAG ATAGCTTCAC CTTCTACCTT 
TGGGATGGCA ACAACAGGTC CCCTGCTCTT GACTGTCAAA TCACCATCAA GGACATGGAA 
AAAGGTGATA TTGTCATCCT TACAAAACCA CTCGTGGTGT CTAAAGGTGA TAGAGGTTTC 
TTAACAACCA CCACGCTCCT GGCTGTGGAC GGAACAGACA AGCCTGAGGA ACTGCTCTAT 
GTCATCACCT CCCCTCCGCG ATATGGCCAG ATCGAATATG TTCACTATCC TGGAGTTCCC 
ATTACAAACT TCAGCCAAAT GGATGTAGTG GGGCAGACAG TTTGCTATGT ACACAAGAGC 
AAGGTGACTG TCTCCAGTGA CAGATTCAGA TTCATCATCA GCAATGGACT GCGGACCGAG 
CACGGGGTGT TTGAGATCAC ACTGGAGACT GTGGACAGAG CCCTGCCTGT GGTAACCAGG 
AACAAGGGGT TGAGACTGGC CCAAGGGGCC GTGGGCCTGC TTTCCCCTGA CCTCCTTCAG 
CTGACCGACC CTGATACACC TGCGGAGAAC CTCACCTTCC TCTTGGTTCA GCTCCCCCAG 
CATGGCCAGC TCTACCTGTG GGGGACAGGG CTACTTCAAC ACAATTTCAC CCAGCAGGAT 
GTGGACAGCA AGAATGTGGC CTATCGGCAC TCAGGAGGGG ACTCCCAGAC TGACTGCTTT 
ACTTTCATGG CCACAGATGG GACAAACCAA GGCTTTATTG TGAATGGGAG AGTGTGGGAA 
GAACCTGTTT TATTCACCAT TCAGGTAGAC CAATTGGACA AAACAGCTCC TCGTATCACA 
CTCTTGCATT CCCCTTCTCA AGTGGGGCTC CTGAAAAATG GCTGCTACGG GATTTACATC 
ACTTCCCGCG TGTTGAAGGC ATCAGACCCT GACACTGAGG ACGATCAGAT CATCTTTAAA 
ATTCTACAAG GCCCAAAACA TGGACATCTG GAGAACACAA CAACAGGTGA ATTTATCCAT 
GAGAAATTTA GCCAAAAGGA CTTAAACAGT AAGACTATTC TTTACATCAT AAACCCATCT 
TTGGAAGTAA ATTCAGATAC CGTGGAATTT CAAATCATGG ACCCCACAGG GAACTCGGCC 
ACTCCTCAAA TTTTGGAACT GAAGTGGTCT CATATTGAAT GGTCACAGAC CGAATATGAA 
GTCTGTGAGA ATGTGGGTTT GTTGCCCTTG GAAATTATCA GAAGGGGATA TTCCATGGAC 
TCGGCCTTTG TGGGTATAAA GGTCAACCAA GTGTCAGCTG CAGTTGGAAA AGATTTCACC 
GTGATTCCAT CTAAACTGAT TCAGTTTGAC CCAGGAATGT CAACTAAGAT GTGGAATATA 
GCAATTACCT ATGACGGATT AGAGGAAGAT GATGAGGTCT TTGAAGTAAT TCTGAACTCC 
CCTGTGAATG CAGTTCTTGG CACAAAGACA AAAGCTGCAG TGAAAATTTT GGACTCAAAA 
GGAGGACAAT GCCATCCTTC ATATTCCTCC AACCAAAGCA AGCACAGCAC ATGGGAGAAG 
GGCATTTGGC ATCTGCTGCC CCCAGGGTCT TCCTCATCCA CCACTTCTGG TTCCTTTCAT 
CTGGAAAGAA GACCTCTTCC ATCTTCCATG CAGCTAGCAG TCATCAGGGG AGACACCCTG 
CGGGGCTTTG ATTCTACAGA TCTTTCTCAA AGGAAGCTTA GGACCCGTGG GAATGGCAAA 
ACAGTTCGTC CATCCTCTGT TTATAGAAAT GGAACAGACA TCATCTATAA TTATCATGGG 
ATAGTTTCCT TGAAACTGGA GGATGACAGT TTCCCAACTC ACAAAAGGAA GGCCAAAGTA 
TCCATCATTA GTCAGCCACA AAAGACAATC AAAGTGGCAG AACTGCCTCA AGCAGATAAG 
GTGGAATCCA CAACTGACTC ACACTTCCCC AGACAGGACC AGTTGCCCTC ATTTCCAAAG 
AACTGCACTC TGGAATTAAA GGGACTCTTC CATTTTGAAG AAGGCATCCA GAAGCTGTAT 
CAGTGCAATG GGATCGCCTG GAAAGCCTGG AGTCCCCAAA CCAAGGATGT GGAAGACAAA 
TCCTGTCCAG CCGGGTGGCA CCAGCACTCA GGCTACTGTC ACATCTTGAT CACAGAGCAG 
AAAGGCACCT GGAATGCGGC TGCCCAAGCT TGCAGGGAAC AATACCTGGG CAACCTTGTA 
ACTGTATTCT CCAGGCAGCA CATGCGGTGG CTCTGGGACA TTGGTGGGAG AAAGTCCTTT 
TGGATAGGTT TGAACGACCA AGTGCATGCT GGCCACTGGG AGTGGATCGG TGGTGAACCT 
GTTGCCTTCA CCAATGGGAG AAGAGGGCCC TCTCAACGCT CCAAGCTTGG AAAGAGCTGT 
GTTTTGGTTC AAAGACAAGG GAAATGGCAA ACAAAAGACT GTAGGAGAGC CAAACCTCAT 
AATTATGTGT GTTCCAGAAA ACTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FREM1 ( XM_017014319.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016869808.1 Homo sapiens FRAS1 related extracellular matrix 1 (FREM1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHu98459
Clone ID Related Accession (Same CDS sequence)	XM_017014320.1 , XM_017014318.1 , XM_017014316.1 , XM_017014316.2 , XM_017014317.1 , XM_017014319.2 , XM_017014321.2 , XM_017014323.1 , XM_017014322.1 , XM_017014321.1 , XM_017014320.2 , XM_017014319.1
Accession Version	XM_017014319.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6567bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	FRAS1-related extracellular matrix protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017014319.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACTCTC TGAGTTGGGG GGCTGCGAAT GCCGTGCTGC TGCTGCTCCT CCTGGCCTGG 
GCCAGCCCCA CCTTCATCAG CATCAACCGC GGGGTGAGGG TGATGAAGGG CCACTCTGCC 
TTCCTGTCAG GAGATGACCT GAAGTTTGCC ATCCCTAAAG AGAAAGATGC CTGCAAAGTG 
GAAGTTGTGA TGAATGAGCC AATAACCCAG AGGGTTGGGA AACTCACTCC ACAGGTCTTT 
GACTGCCATT TCCTTCCCAA CGAAGTCAAG TATGTTCACA ATGGTTGTCC AATTCTTGAT 
GAAGACACAG TGAAGCTCAG ACTTTACAGA TTTACTGAAA GAGATACCTT CATAGAAACT 
TTTATCCTGT GGGTCTATCT CCTGGAACCA GACTGTAACA TCATCCATAT GAGTAACAAT 
GTGCTGGAGG TGCCTGAATT CAATGGCTTG TCCCAAGCGA TTGATAAAAA TCTGCTCAGA 
TTCGATTATG ATAGGATGGC TAGCCTGGAA TGTACCGTCA GCCTGGACAC TGCGAGAACT 
CGGCTGCCAG CCCATGGCCA GATGGTTCTC GGGGAGCCTC GACCAGAAGA ACCTCGTGGA 
GATCAGCCAC ACAGTTTTTT CCCAGAGTCC CGTATGATGT CTTTTAATGC CTTTCTGGAA 
CTGAGAGCAA AACTGAAGTG TCCAGGTGGG AGCTGTACCC CAGGATTAAA GAAAATAGGA 
AGCCTCAAAG TGAGCTGTGA GGAGTTCCTG CTGATGGGCC TTCGTTATCA GCATCTGGAT 
CCCCCTTCAC CCAACATTGA TTATATCTCC ATCCAACTGG ACCTCACAGA TACCAGGAGC 
AAAATTGTGT ACAAGTCAGA GAGTGCGTGG CTGCCTGTCT ATATCAGAGC TGGAATTCCG 
AATCAGATTC CAAAGGCTGC ATTCATGGCC GTGTTTATTC TGGAAGTGGA TCAGTTCATC 
CTGACCTCCT TGACTACATC AGTTCTGGAC TGTGAAGAAG ATGAGACCCC TAAACCCTTG 
CTGGTGTTCA ACATTACTAA AGCCCCGCTC CAGGGCTATG TGACTCACCT GTTGGATCAC 
ACCAGACCAA TCTCCTCATT CACCTGGAAA GATCTCAGTG ACATGCAGAT CGCCTATCAG 
CCACCAAACA GCAGCCATTC TGAGAGGAGA CATGATGAGG TAGAATTGGA GGTATACGAC 
TTCTTCTTTG AAAGGAGTGC ACCTATGACA GTCCACATCT CCATCAGAAC AGCAGATACA 
AATGCCCCCC GTGTATCCTG GAATACAGGT CTGAGTCTCC TTGAGGGGCA GTCTCGAGCC 
ATCACTTGGG AACAGTTTCA GGTTGTCGAC AATGACGACA TTGGTGCTGT CCGGCTAGTC 
ACCGTTGGTG GCCTGCAGCA TGGATGGCTG ACTTTAAGAG GGGGGAAAGG GTTTCTCTTC 
ACCGTGGCTG ACCTCCAGGC TGGAGTTGTT CGCTATCATC ATGATGACAG CGACTCCACC 
AAAGACTTCG TGGTCTTCCG GATATTTGAT GGCCATCACA GCATCCGTCA CAAATTCCCC 
ATCAACGTCT TGCCCAAAGA TGATAGTCCC CCGTTCCTCA TAACCAATGT TGTGATTGAA 
CTGGAGGAGG GGCAGACCAT CCTGATCCAG GGATCCATGC TGCGAGCTTC AGATGTGGAC 
GCCAGTGATG ACTACATCTT CTTCAATATC ACAAAGCCTC CACAGGCTGG GGAGATCATG 
AAGAAGCCAG GGCCAGGACT GATAGGCTAT CCTGTCCATG GCTTCCTTCA GAGGGATTTG 
TTTAATGGAA TCATTTATTA TCGTCATTTT GGTGGAGAAA TCTTTGAAGA TTCTTTTCAA 
TTTGTCCTGT GGGACAGCCA TGAACCTCCA AATCTTTCAG TGCCACAGGT GGCAACAATC 
CATATAACTC CAGTGGATGA CCAGCTTCCA AAAGAGGCTC CTGGAGTTTC TCGGCATTTG 
GTTGTCAAGG AAACTGAGGT GGCCTATATA ACTAAGAAAC AGCTACATTT TATAGATTCA 
GAATCATATG ACAGGGAGCT GGTCTACACA ATAACTACTC CTCCATTTTT CTCCTTCAGC 
CACAGACACT TGGATGCTGG GAAATTATTT ATGGTGGACA GCATACCAAA AGTAGTTAAG 
AATCCTACGG CCCTGGAGCT GAGGTCATTC ACTCAGCATG CTGTGAACTA TATGAAAGTG 
GCCTACATGC CCCCCATGCA AGACATTGGT CCCCATTGCA GAGATGTCCA GTTCACATTT 
TCTGTCAGTA ACCAACATGG CGGTACTTTG CATGGGATCT GCTTTAACAT TACAATCCTC 
CCAGTGGACA ATCAAGTTCC TGAGGCGTTC ACCAACCCTC TGAAAGTGAC TGAGGGAGGT 
CAAAGCATCA TCAGCACAGA GCACATTCTA ATTTCTGATG CAGATACCAA GCTGGACAAT 
ATTGACCTCT CCCTGCGGGA ATTGCCTCTG CACGGAAGGG TGGAGCTGAA TGGATTTCCT 
CTAAATTCAG GGGGCACATT TTCTTGGGGC GATCTCCATA CCTTAAAAGT TAGGTATCAA 
CATGATGGAA CTGAAGTTCT TCAGGATGAC CTACTCTTGG AGGTCACCGA TGGCACAAAT 
TCAGCAGAAT TTGTACTACA TGTTGAGGTA TTCCCTGTCA ACGATGAGCC ACCAGTCTTA 
AAGGCTGACC TCATGCCTGT CATGAATTGC TCAGAGGGAG GAGAGGTGGT CATCACCTCT 
GAATACATTT TTGCTACTGA TGTGGACAGC GATAACTTGA AGTTGATGTT TGTGATTGCT 
CGCGAACCTC AGCATGGGGT GGTGAGGAGA GCTGGAGTCA CAGTGGATCA GTTCTCTCAG 
AGAGATGTTA TCTCAGAGGC CGTGACATAC AAACACACAG GTGGCGAGAT TGGCCTGATG 
CCTTGTTTTG ACACCATTAC ATTGGTGGTT TCGGATGGAG AGGCTGGCCC TTTTGTGAAT 
GGCTGTTGCT ACAATGGACC CAATCCATCT GTTCCACTTC ATGCGTCCTT TCCAGTGTAT 
GATCTCAACA TCACGGTATA CCCAGTAGAC AACCAGCCAC CTTCCATTGC AATAGGTCCC 
GTGTTTGTTG TAGATGAGGG CTGCTCAACA GCCCTTACTG TTAACCATTT GTCCGCCACT 
GACCCTGACA CTGCAGCAGA TGACTTGGAA TTTGTTTTGG TTTCTCCTCC TCAGTTTGGC 
TACCTCGAAA ATATACTCCC TTCTGTGGGT TTTGAAAAAA GCAATATTGG CATAAGTATA 
GATTCATTTC AGTGGAAAGA CATGAACGCT TTTCACATTA ACTATGTGCA GTCCAGGCAT 
CTGAGGATAG AACCAACTGC CGACCAGTTC ACGGTGTACG TCACAGATGG GAAGCATCAC 
TCCTTGGAGA TACCATTTTC TATTATAATC AACCCCACAA ATGATGAAGC TCCTGACTTT 
GTAGTGCAGA ATATTACTGT GTGTGAGGGT CAGATGAAAG AGCTGGACTC TTCCATCATC 
AGCGCTGTGG ACCTGGACAT TCCCCAGGAT GCCCTGCTGT TCAGCATCAC TCAAAAGCCA 
CGCCATGGCC TCCTCATCGA TAGGGGGTTT AGCAAAGACT TCTCTGAGAA TAAGCAGCCA 
GCCAACCCTC ACCAGAAACA TGCACCTGTT CACAGCTTTT CCATGGAACT CCTCAAGACT 
GGAATGAGGT TGACGTACAT GCATGATGAC TCAGAGAGCC TTGCTGATGA TTTTACAATC 
CAATTGTCAG ATGGGAAACA TAAGATACTT AAAACCATTT CAGTAGAGGT CATCCCAGTT 
AATGATGAAA AACCAATGCT GAGCAAGAAG GCTGAAATTG CAATGAATAT GGGTGAAACT 
CGTATTATTT CCAGTGCTAT TCTTTCAGCC ATAGATGAAG ACTCACCCAG GGAGAAGATT 
TACTATGTAT TTGAAAGGCT TCCCCAAAAT GGGCAACTTC AGCTTAAGAT AGGGAGGGAC 
TGGGTTCCTC TCTCCCCTGG CATGAAATGC ACTCAGGAGG AAGTGGATCT GAACTTGCTG 
AGATACACAC ACACCGGGGC AATGGATTCC CAGAATCAAG ATAGCTTCAC CTTCTACCTT 
TGGGATGGCA ACAACAGGTC CCCTGCTCTT GACTGTCAAA TCACCATCAA GGACATGGAA 
AAAGGTGATA TTGTCATCCT TACAAAACCA CTCGTGGTGT CTAAAGGTGA TAGAGGTTTC 
TTAACAACCA CCACGCTCCT GGCTGTGGAC GGAACAGACA AGCCTGAGGA ACTGCTCTAT 
GTCATCACCT CCCCTCCGCG ATATGGCCAG ATCGAATATG TTCACTATCC TGGAGTTCCC 
ATTACAAACT TCAGCCAAAT GGATGTAGTG GGGCAGACAG TTTGCTATGT ACACAAGAGC 
AAGGTGACTG TCTCCAGTGA CAGATTCAGA TTCATCATCA GCAATGGACT GCGGACCGAG 
CACGGGGTGT TTGAGATCAC ACTGGAGACT GTGGACAGAG CCCTGCCTGT GGTAACCAGG 
AACAAGGGGT TGAGACTGGC CCAAGGGGCC GTGGGCCTGC TTTCCCCTGA CCTCCTTCAG 
CTGACCGACC CTGATACACC TGCGGAGAAC CTCACCTTCC TCTTGGTTCA GCTCCCCCAG 
CATGGCCAGC TCTACCTGTG GGGGACAGGG CTACTTCAAC ACAATTTCAC CCAGCAGGAT 
GTGGACAGCA AGAATGTGGC CTATCGGCAC TCAGGAGGGG ACTCCCAGAC TGACTGCTTT 
ACTTTCATGG CCACAGATGG GACAAACCAA GGCTTTATTG TGAATGGGAG AGTGTGGGAA 
GAACCTGTTT TATTCACCAT TCAGGTAGAC CAATTGGACA AAACAGCTCC TCGTATCACA 
CTCTTGCATT CCCCTTCTCA AGTGGGGCTC CTGAAAAATG GCTGCTACGG GATTTACATC 
ACTTCCCGCG TGTTGAAGGC ATCAGACCCT GACACTGAGG ACGATCAGAT CATCTTTAAA 
ATTCTACAAG GCCCAAAACA TGGACATCTG GAGAACACAA CAACAGGTGA ATTTATCCAT 
GAGAAATTTA GCCAAAAGGA CTTAAACAGT AAGACTATTC TTTACATCAT AAACCCATCT 
TTGGAAGTAA ATTCAGATAC CGTGGAATTT CAAATCATGG ACCCCACAGG GAACTCGGCC 
ACTCCTCAAA TTTTGGAACT GAAGTGGTCT CATATTGAAT GGTCACAGAC CGAATATGAA 
GTCTGTGAGA ATGTGGGTTT GTTGCCCTTG GAAATTATCA GAAGGGGATA TTCCATGGAC 
TCGGCCTTTG TGGGTATAAA GGTCAACCAA GTGTCAGCTG CAGTTGGAAA AGATTTCACC 
GTGATTCCAT CTAAACTGAT TCAGTTTGAC CCAGGAATGT CAACTAAGAT GTGGAATATA 
GCAATTACCT ATGACGGATT AGAGGAAGAT GATGAGGTCT TTGAAGTAAT TCTGAACTCC 
CCTGTGAATG CAGTTCTTGG CACAAAGACA AAAGCTGCAG TGAAAATTTT GGACTCAAAA 
GGAGGACAAT GCCATCCTTC ATATTCCTCC AACCAAAGCA AGCACAGCAC ATGGGAGAAG 
GGCATTTGGC ATCTGCTGCC CCCAGGGTCT TCCTCATCCA CCACTTCTGG TTCCTTTCAT 
CTGGAAAGAA GACCTCTTCC ATCTTCCATG CAGCTAGCAG TCATCAGGGG AGACACCCTG 
CGGGGCTTTG ATTCTACAGA TCTTTCTCAA AGGAAGCTTA GGACCCGTGG GAATGGCAAA 
ACAGTTCGTC CATCCTCTGT TTATAGAAAT GGAACAGACA TCATCTATAA TTATCATGGG 
ATAGTTTCCT TGAAACTGGA GGATGACAGT TTCCCAACTC ACAAAAGGAA GGCCAAAGTA 
TCCATCATTA GTCAGCCACA AAAGACAATC AAAGTGGCAG AACTGCCTCA AGCAGATAAG 
GTGGAATCCA CAACTGACTC ACACTTCCCC AGACAGGACC AGTTGCCCTC ATTTCCAAAG 
AACTGCACTC TGGAATTAAA GGGACTCTTC CATTTTGAAG AAGGCATCCA GAAGCTGTAT 
CAGTGCAATG GGATCGCCTG GAAAGCCTGG AGTCCCCAAA CCAAGGATGT GGAAGACAAA 
TCCTGTCCAG CCGGGTGGCA CCAGCACTCA GGCTACTGTC ACATCTTGAT CACAGAGCAG 
AAAGGCACCT GGAATGCGGC TGCCCAAGCT TGCAGGGAAC AATACCTGGG CAACCTTGTA 
ACTGTATTCT CCAGGCAGCA CATGCGGTGG CTCTGGGACA TTGGTGGGAG AAAGTCCTTT 
TGGATAGGTT TGAACGACCA AGTGCATGCT GGCCACTGGG AGTGGATCGG TGGTGAACCT 
GTTGCCTTCA CCAATGGGAG AAGAGGGCCC TCTCAACGCT CCAAGCTTGG AAAGAGCTGT 
GTTTTGGTTC AAAGACAAGG GAAATGGCAA ACAAAAGACT GTAGGAGAGC CAAACCTCAT 
AATTATGTGT GTTCCAGAAA ACTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016869808.1
CDS	1262..7828
Translation	MNSLSWGAANAVLLLLLLAWASPTFISINRGVRVMKGHSAFLSGDDLKFAIPKEKDACKVEVVMNEPITQRVGKLTPQVFDCHFLPNEVKYVHNGCPILDEDTVKLRLYRFTERDTFIETFILWVYLLEPDCNIIHMSNNVLEVPEFNGLSQAIDKNLLRFDYDRMASLECTVSLDTARTRLPAHGQMVLGEPRPEEPRGDQPHSFFPESRMMSFNAFLELRAKLKCPGGSCTPGLKKIGSLKVSCEEFLLMGLRYQHLDPPSPNIDYISIQLDLTDTRSKIVYKSESAWLPVYIRAGIPNQIPKAAFMAVFILEVDQFILTSLTTSVLDCEEDETPKPLLVFNITKAPLQGYVTHLLDHTRPISSFTWKDLSDMQIAYQPPNSSHSERRHDEVELEVYDFFFERSAPMTVHISIRTADTNAPRVSWNTGLSLLEGQSRAITWEQFQVVDNDDIGAVRLVTVGGLQHGWLTLRGGKGFLFTVADLQAGVVRYHHDDSDSTKDFVVFRIFDGHHSIRHKFPINVLPKDDSPPFLITNVVIELEEGQTILIQGSMLRASDVDASDDYIFFNITKPPQAGEIMKKPGPGLIGYPVHGFLQRDLFNGIIYYRHFGGEIFEDSFQFVLWDSHEPPNLSVPQVATIHITPVDDQLPKEAPGVSRHLVVKETEVAYITKKQLHFIDSESYDRELVYTITTPPFFSFSHRHLDAGKLFMVDSIPKVVKNPTALELRSFTQHAVNYMKVAYMPPMQDIGPHCRDVQFTFSVSNQHGGTLHGICFNITILPVDNQVPEAFTNPLKVTEGGQSIISTEHILISDADTKLDNIDLSLRELPLHGRVELNGFPLNSGGTFSWGDLHTLKVRYQHDGTEVLQDDLLLEVTDGTNSAEFVLHVEVFPVNDEPPVLKADLMPVMNCSEGGEVVITSEYIFATDVDSDNLKLMFVIAREPQHGVVRRAGVTVDQFSQRDVISEAVTYKHTGGEIGLMPCFDTITLVVSDGEAGPFVNGCCYNGPNPSVPLHASFPVYDLNITVYPVDNQPPSIAIGPVFVVDEGCSTALTVNHLSATDPDTAADDLEFVLVSPPQFGYLENILPSVGFEKSNIGISIDSFQWKDMNAFHINYVQSRHLRIEPTADQFTVYVTDGKHHSLEIPFSIIINPTNDEAPDFVVQNITVCEGQMKELDSSIISAVDLDIPQDALLFSITQKPRHGLLIDRGFSKDFSENKQPANPHQKHAPVHSFSMELLKTGMRLTYMHDDSESLADDFTIQLSDGKHKILKTISVEVIPVNDEKPMLSKKAEIAMNMGETRIISSAILSAIDEDSPREKIYYVFERLPQNGQLQLKIGRDWVPLSPGMKCTQEEVDLNLLRYTHTGAMDSQNQDSFTFYLWDGNNRSPALDCQITIKDMEKGDIVILTKPLVVSKGDRGFLTTTTLLAVDGTDKPEELLYVITSPPRYGQIEYVHYPGVPITNFSQMDVVGQTVCYVHKSKVTVSSDRFRFIISNGLRTEHGVFEITLETVDRALPVVTRNKGLRLAQGAVGLLSPDLLQLTDPDTPAENLTFLLVQLPQHGQLYLWGTGLLQHNFTQQDVDSKNVAYRHSGGDSQTDCFTFMATDGTNQGFIVNGRVWEEPVLFTIQVDQLDKTAPRITLLHSPSQVGLLKNGCYGIYITSRVLKASDPDTEDDQIIFKILQGPKHGHLENTTTGEFIHEKFSQKDLNSKTILYIINPSLEVNSDTVEFQIMDPTGNSATPQILELKWSHIEWSQTEYEVCENVGLLPLEIIRRGYSMDSAFVGIKVNQVSAAVGKDFTVIPSKLIQFDPGMSTKMWNIAITYDGLEEDDEVFEVILNSPVNAVLGTKTKAAVKILDSKGGQCHPSYSSNQSKHSTWEKGIWHLLPPGSSSSTTSGSFHLERRPLPSSMQLAVIRGDTLRGFDSTDLSQRKLRTRGNGKTVRPSSVYRNGTDIIYNYHGIVSLKLEDDSFPTHKRKAKVSIISQPQKTIKVAELPQADKVESTTDSHFPRQDQLPSFPKNCTLELKGLFHFEEGIQKLYQCNGIAWKAWSPQTKDVEDKSCPAGWHQHSGYCHILITEQKGTWNAAAQACREQYLGNLVTVFSRQHMRWLWDIGGRKSFWIGLNDQVHAGHWEWIGGEPVAFTNGRRGPSQRSKLGKSCVLVQRQGKWQTKDCRRAKPHNYVCSRKL

Target ORF information:

RefSeq Version	XM_017014319.2
Organism	Homo sapiens(human)
Definition	Homo sapiens FRAS1 related extracellular matrix 1 (FREM1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017014319.2

ORF Insert Sequence:

ATGAACTCTC TGAGTTGGGG GGCTGCGAAT GCCGTGCTGC TGCTGCTCCT CCTGGCCTGG 
GCCAGCCCCA CCTTCATCAG CATCAACCGC GGGGTGAGGG TGATGAAGGG CCACTCTGCC 
TTCCTGTCAG GAGATGACCT GAAGTTTGCC ATCCCTAAAG AGAAAGATGC CTGCAAAGTG 
GAAGTTGTGA TGAATGAGCC AATAACCCAG AGGGTTGGGA AACTCACTCC ACAGGTCTTT 
GACTGCCATT TCCTTCCCAA CGAAGTCAAG TATGTTCACA ATGGTTGTCC AATTCTTGAT 
GAAGACACAG TGAAGCTCAG ACTTTACAGA TTTACTGAAA GAGATACCTT CATAGAAACT 
TTTATCCTGT GGGTCTATCT CCTGGAACCA GACTGTAACA TCATCCATAT GAGTAACAAT 
GTGCTGGAGG TGCCTGAATT CAATGGCTTG TCCCAAGCGA TTGATAAAAA TCTGCTCAGA 
TTCGATTATG ATAGGATGGC TAGCCTGGAA TGTACCGTCA GCCTGGACAC TGCGAGAACT 
CGGCTGCCAG CCCATGGCCA GATGGTTCTC GGGGAGCCTC GACCAGAAGA ACCTCGTGGA 
GATCAGCCAC ACAGTTTTTT CCCAGAGTCC CGTATGATGT CTTTTAATGC CTTTCTGGAA 
CTGAGAGCAA AACTGAAGTG TCCAGGTGGG AGCTGTACCC CAGGATTAAA GAAAATAGGA 
AGCCTCAAAG TGAGCTGTGA GGAGTTCCTG CTGATGGGCC TTCGTTATCA GCATCTGGAT 
CCCCCTTCAC CCAACATTGA TTATATCTCC ATCCAACTGG ACCTCACAGA TACCAGGAGC 
AAAATTGTGT ACAAGTCAGA GAGTGCGTGG CTGCCTGTCT ATATCAGAGC TGGAATTCCG 
AATCAGATTC CAAAGGCTGC ATTCATGGCC GTGTTTATTC TGGAAGTGGA TCAGTTCATC 
CTGACCTCCT TGACTACATC AGTTCTGGAC TGTGAAGAAG ATGAGACCCC TAAACCCTTG 
CTGGTGTTCA ACATTACTAA AGCCCCGCTC CAGGGCTATG TGACTCACCT GTTGGATCAC 
ACCAGACCAA TCTCCTCATT CACCTGGAAA GATCTCAGTG ACATGCAGAT CGCCTATCAG 
CCACCAAACA GCAGCCATTC TGAGAGGAGA CATGATGAGG TAGAATTGGA GGTATACGAC 
TTCTTCTTTG AAAGGAGTGC ACCTATGACA GTCCACATCT CCATCAGAAC AGCAGATACA 
AATGCCCCCC GTGTATCCTG GAATACAGGT CTGAGTCTCC TTGAGGGGCA GTCTCGAGCC 
ATCACTTGGG AACAGTTTCA GGTTGTCGAC AATGACGACA TTGGTGCTGT CCGGCTAGTC 
ACCGTTGGTG GCCTGCAGCA TGGATGGCTG ACTTTAAGAG GGGGGAAAGG GTTTCTCTTC 
ACCGTGGCTG ACCTCCAGGC TGGAGTTGTT CGCTATCATC ATGATGACAG CGACTCCACC 
AAAGACTTCG TGGTCTTCCG GATATTTGAT GGCCATCACA GCATCCGTCA CAAATTCCCC 
ATCAACGTCT TGCCCAAAGA TGATAGTCCC CCGTTCCTCA TAACCAATGT TGTGATTGAA 
CTGGAGGAGG GGCAGACCAT CCTGATCCAG GGATCCATGC TGCGAGCTTC AGATGTGGAC 
GCCAGTGATG ACTACATCTT CTTCAATATC ACAAAGCCTC CACAGGCTGG GGAGATCATG 
AAGAAGCCAG GGCCAGGACT GATAGGCTAT CCTGTCCATG GCTTCCTTCA GAGGGATTTG 
TTTAATGGAA TCATTTATTA TCGTCATTTT GGTGGAGAAA TCTTTGAAGA TTCTTTTCAA 
TTTGTCCTGT GGGACAGCCA TGAACCTCCA AATCTTTCAG TGCCACAGGT GGCAACAATC 
CATATAACTC CAGTGGATGA CCAGCTTCCA AAAGAGGCTC CTGGAGTTTC TCGGCATTTG 
GTTGTCAAGG AAACTGAGGT GGCCTATATA ACTAAGAAAC AGCTACATTT TATAGATTCA 
GAATCATATG ACAGGGAGCT GGTCTACACA ATAACTACTC CTCCATTTTT CTCCTTCAGC 
CACAGACACT TGGATGCTGG GAAATTATTT ATGGTGGACA GCATACCAAA AGTAGTTAAG 
AATCCTACGG CCCTGGAGCT GAGGTCATTC ACTCAGCATG CTGTGAACTA TATGAAAGTG 
GCCTACATGC CCCCCATGCA AGACATTGGT CCCCATTGCA GAGATGTCCA GTTCACATTT 
TCTGTCAGTA ACCAACATGG CGGTACTTTG CATGGGATCT GCTTTAACAT TACAATCCTC 
CCAGTGGACA ATCAAGTTCC TGAGGCGTTC ACCAACCCTC TGAAAGTGAC TGAGGGAGGT 
CAAAGCATCA TCAGCACAGA GCACATTCTA ATTTCTGATG CAGATACCAA GCTGGACAAT 
ATTGACCTCT CCCTGCGGGA ATTGCCTCTG CACGGAAGGG TGGAGCTGAA TGGATTTCCT 
CTAAATTCAG GGGGCACATT TTCTTGGGGC GATCTCCATA CCTTAAAAGT TAGGTATCAA 
CATGATGGAA CTGAAGTTCT TCAGGATGAC CTACTCTTGG AGGTCACCGA TGGCACAAAT 
TCAGCAGAAT TTGTACTACA TGTTGAGGTA TTCCCTGTCA ACGATGAGCC ACCAGTCTTA 
AAGGCTGACC TCATGCCTGT CATGAATTGC TCAGAGGGAG GAGAGGTGGT CATCACCTCT 
GAATACATTT TTGCTACTGA TGTGGACAGC GATAACTTGA AGTTGATGTT TGTGATTGCT 
CGCGAACCTC AGCATGGGGT GGTGAGGAGA GCTGGAGTCA CAGTGGATCA GTTCTCTCAG 
AGAGATGTTA TCTCAGAGGC CGTGACATAC AAACACACAG GTGGCGAGAT TGGCCTGATG 
CCTTGTTTTG ACACCATTAC ATTGGTGGTT TCGGATGGAG AGGCTGGCCC TTTTGTGAAT 
GGCTGTTGCT ACAATGGACC CAATCCATCT GTTCCACTTC ATGCGTCCTT TCCAGTGTAT 
GATCTCAACA TCACGGTATA CCCAGTAGAC AACCAGCCAC CTTCCATTGC AATAGGTCCC 
GTGTTTGTTG TAGATGAGGG CTGCTCAACA GCCCTTACTG TTAACCATTT GTCCGCCACT 
GACCCTGACA CTGCAGCAGA TGACTTGGAA TTTGTTTTGG TTTCTCCTCC TCAGTTTGGC 
TACCTCGAAA ATATACTCCC TTCTGTGGGT TTTGAAAAAA GCAATATTGG CATAAGTATA 
GATTCATTTC AGTGGAAAGA CATGAACGCT TTTCACATTA ACTATGTGCA GTCCAGGCAT 
CTGAGGATAG AACCAACTGC CGACCAGTTC ACGGTGTACG TCACAGATGG GAAGCATCAC 
TCCTTGGAGA TACCATTTTC TATTATAATC AACCCCACAA ATGATGAAGC TCCTGACTTT 
GTAGTGCAGA ATATTACTGT GTGTGAGGGT CAGATGAAAG AGCTGGACTC TTCCATCATC 
AGCGCTGTGG ACCTGGACAT TCCCCAGGAT GCCCTGCTGT TCAGCATCAC TCAAAAGCCA 
CGCCATGGCC TCCTCATCGA TAGGGGGTTT AGCAAAGACT TCTCTGAGAA TAAGCAGCCA 
GCCAACCCTC ACCAGAAACA TGCACCTGTT CACAGCTTTT CCATGGAACT CCTCAAGACT 
GGAATGAGGT TGACGTACAT GCATGATGAC TCAGAGAGCC TTGCTGATGA TTTTACAATC 
CAATTGTCAG ATGGGAAACA TAAGATACTT AAAACCATTT CAGTAGAGGT CATCCCAGTT 
AATGATGAAA AACCAATGCT GAGCAAGAAG GCTGAAATTG CAATGAATAT GGGTGAAACT 
CGTATTATTT CCAGTGCTAT TCTTTCAGCC ATAGATGAAG ACTCACCCAG GGAGAAGATT 
TACTATGTAT TTGAAAGGCT TCCCCAAAAT GGGCAACTTC AGCTTAAGAT AGGGAGGGAC 
TGGGTTCCTC TCTCCCCTGG CATGAAATGC ACTCAGGAGG AAGTGGATCT GAACTTGCTG 
AGATACACAC ACACCGGGGC AATGGATTCC CAGAATCAAG ATAGCTTCAC CTTCTACCTT 
TGGGATGGCA ACAACAGGTC CCCTGCTCTT GACTGTCAAA TCACCATCAA GGACATGGAA 
AAAGGTGATA TTGTCATCCT TACAAAACCA CTCGTGGTGT CTAAAGGTGA TAGAGGTTTC 
TTAACAACCA CCACGCTCCT GGCTGTGGAC GGAACAGACA AGCCTGAGGA ACTGCTCTAT 
GTCATCACCT CCCCTCCGCG ATATGGCCAG ATCGAATATG TTCACTATCC TGGAGTTCCC 
ATTACAAACT TCAGCCAAAT GGATGTAGTG GGGCAGACAG TTTGCTATGT ACACAAGAGC 
AAGGTGACTG TCTCCAGTGA CAGATTCAGA TTCATCATCA GCAATGGACT GCGGACCGAG 
CACGGGGTGT TTGAGATCAC ACTGGAGACT GTGGACAGAG CCCTGCCTGT GGTAACCAGG 
AACAAGGGGT TGAGACTGGC CCAAGGGGCC GTGGGCCTGC TTTCCCCTGA CCTCCTTCAG 
CTGACCGACC CTGATACACC TGCGGAGAAC CTCACCTTCC TCTTGGTTCA GCTCCCCCAG 
CATGGCCAGC TCTACCTGTG GGGGACAGGG CTACTTCAAC ACAATTTCAC CCAGCAGGAT 
GTGGACAGCA AGAATGTGGC CTATCGGCAC TCAGGAGGGG ACTCCCAGAC TGACTGCTTT 
ACTTTCATGG CCACAGATGG GACAAACCAA GGCTTTATTG TGAATGGGAG AGTGTGGGAA 
GAACCTGTTT TATTCACCAT TCAGGTAGAC CAATTGGACA AAACAGCTCC TCGTATCACA 
CTCTTGCATT CCCCTTCTCA AGTGGGGCTC CTGAAAAATG GCTGCTACGG GATTTACATC 
ACTTCCCGCG TGTTGAAGGC ATCAGACCCT GACACTGAGG ACGATCAGAT CATCTTTAAA 
ATTCTACAAG GCCCAAAACA TGGACATCTG GAGAACACAA CAACAGGTGA ATTTATCCAT 
GAGAAATTTA GCCAAAAGGA CTTAAACAGT AAGACTATTC TTTACATCAT AAACCCATCT 
TTGGAAGTAA ATTCAGATAC CGTGGAATTT CAAATCATGG ACCCCACAGG GAACTCGGCC 
ACTCCTCAAA TTTTGGAACT GAAGTGGTCT CATATTGAAT GGTCACAGAC CGAATATGAA 
GTCTGTGAGA ATGTGGGTTT GTTGCCCTTG GAAATTATCA GAAGGGGATA TTCCATGGAC 
TCGGCCTTTG TGGGTATAAA GGTCAACCAA GTGTCAGCTG CAGTTGGAAA AGATTTCACC 
GTGATTCCAT CTAAACTGAT TCAGTTTGAC CCAGGAATGT CAACTAAGAT GTGGAATATA 
GCAATTACCT ATGACGGATT AGAGGAAGAT GATGAGGTCT TTGAAGTAAT TCTGAACTCC 
CCTGTGAATG CAGTTCTTGG CACAAAGACA AAAGCTGCAG TGAAAATTTT GGACTCAAAA 
GGAGGACAAT GCCATCCTTC ATATTCCTCC AACCAAAGCA AGCACAGCAC ATGGGAGAAG 
GGCATTTGGC ATCTGCTGCC CCCAGGGTCT TCCTCATCCA CCACTTCTGG TTCCTTTCAT 
CTGGAAAGAA GACCTCTTCC ATCTTCCATG CAGCTAGCAG TCATCAGGGG AGACACCCTG 
CGGGGCTTTG ATTCTACAGA TCTTTCTCAA AGGAAGCTTA GGACCCGTGG GAATGGCAAA 
ACAGTTCGTC CATCCTCTGT TTATAGAAAT GGAACAGACA TCATCTATAA TTATCATGGG 
ATAGTTTCCT TGAAACTGGA GGATGACAGT TTCCCAACTC ACAAAAGGAA GGCCAAAGTA 
TCCATCATTA GTCAGCCACA AAAGACAATC AAAGTGGCAG AACTGCCTCA AGCAGATAAG 
GTGGAATCCA CAACTGACTC ACACTTCCCC AGACAGGACC AGTTGCCCTC ATTTCCAAAG 
AACTGCACTC TGGAATTAAA GGGACTCTTC CATTTTGAAG AAGGCATCCA GAAGCTGTAT 
CAGTGCAATG GGATCGCCTG GAAAGCCTGG AGTCCCCAAA CCAAGGATGT GGAAGACAAA 
TCCTGTCCAG CCGGGTGGCA CCAGCACTCA GGCTACTGTC ACATCTTGAT CACAGAGCAG 
AAAGGCACCT GGAATGCGGC TGCCCAAGCT TGCAGGGAAC AATACCTGGG CAACCTTGTA 
ACTGTATTCT CCAGGCAGCA CATGCGGTGG CTCTGGGACA TTGGTGGGAG AAAGTCCTTT 
TGGATAGGTT TGAACGACCA AGTGCATGCT GGCCACTGGG AGTGGATCGG TGGTGAACCT 
GTTGCCTTCA CCAATGGGAG AAGAGGGCCC TCTCAACGCT CCAAGCTTGG AAAGAGCTGT 
GTTTTGGTTC AAAGACAAGG GAAATGGCAA ACAAAAGACT GTAGGAGAGC CAAACCTCAT 
AATTATGTGT GTTCCAGAAA ACTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FREM1 ( XM_017014321.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016869810.1 Homo sapiens FRAS1 related extracellular matrix 1 (FREM1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OHu98459
Clone ID Related Accession (Same CDS sequence)	XM_017014320.1 , XM_017014318.1 , XM_017014316.1 , XM_017014316.2 , XM_017014317.1 , XM_017014319.2 , XM_017014321.2 , XM_017014323.1 , XM_017014322.1 , XM_017014321.1 , XM_017014320.2 , XM_017014319.1
Accession Version	XM_017014321.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6567bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	FRAS1-related extracellular matrix protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017014321.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACTCTC TGAGTTGGGG GGCTGCGAAT GCCGTGCTGC TGCTGCTCCT CCTGGCCTGG 
GCCAGCCCCA CCTTCATCAG CATCAACCGC GGGGTGAGGG TGATGAAGGG CCACTCTGCC 
TTCCTGTCAG GAGATGACCT GAAGTTTGCC ATCCCTAAAG AGAAAGATGC CTGCAAAGTG 
GAAGTTGTGA TGAATGAGCC AATAACCCAG AGGGTTGGGA AACTCACTCC ACAGGTCTTT 
GACTGCCATT TCCTTCCCAA CGAAGTCAAG TATGTTCACA ATGGTTGTCC AATTCTTGAT 
GAAGACACAG TGAAGCTCAG ACTTTACAGA TTTACTGAAA GAGATACCTT CATAGAAACT 
TTTATCCTGT GGGTCTATCT CCTGGAACCA GACTGTAACA TCATCCATAT GAGTAACAAT 
GTGCTGGAGG TGCCTGAATT CAATGGCTTG TCCCAAGCGA TTGATAAAAA TCTGCTCAGA 
TTCGATTATG ATAGGATGGC TAGCCTGGAA TGTACCGTCA GCCTGGACAC TGCGAGAACT 
CGGCTGCCAG CCCATGGCCA GATGGTTCTC GGGGAGCCTC GACCAGAAGA ACCTCGTGGA 
GATCAGCCAC ACAGTTTTTT CCCAGAGTCC CGTATGATGT CTTTTAATGC CTTTCTGGAA 
CTGAGAGCAA AACTGAAGTG TCCAGGTGGG AGCTGTACCC CAGGATTAAA GAAAATAGGA 
AGCCTCAAAG TGAGCTGTGA GGAGTTCCTG CTGATGGGCC TTCGTTATCA GCATCTGGAT 
CCCCCTTCAC CCAACATTGA TTATATCTCC ATCCAACTGG ACCTCACAGA TACCAGGAGC 
AAAATTGTGT ACAAGTCAGA GAGTGCGTGG CTGCCTGTCT ATATCAGAGC TGGAATTCCG 
AATCAGATTC CAAAGGCTGC ATTCATGGCC GTGTTTATTC TGGAAGTGGA TCAGTTCATC 
CTGACCTCCT TGACTACATC AGTTCTGGAC TGTGAAGAAG ATGAGACCCC TAAACCCTTG 
CTGGTGTTCA ACATTACTAA AGCCCCGCTC CAGGGCTATG TGACTCACCT GTTGGATCAC 
ACCAGACCAA TCTCCTCATT CACCTGGAAA GATCTCAGTG ACATGCAGAT CGCCTATCAG 
CCACCAAACA GCAGCCATTC TGAGAGGAGA CATGATGAGG TAGAATTGGA GGTATACGAC 
TTCTTCTTTG AAAGGAGTGC ACCTATGACA GTCCACATCT CCATCAGAAC AGCAGATACA 
AATGCCCCCC GTGTATCCTG GAATACAGGT CTGAGTCTCC TTGAGGGGCA GTCTCGAGCC 
ATCACTTGGG AACAGTTTCA GGTTGTCGAC AATGACGACA TTGGTGCTGT CCGGCTAGTC 
ACCGTTGGTG GCCTGCAGCA TGGATGGCTG ACTTTAAGAG GGGGGAAAGG GTTTCTCTTC 
ACCGTGGCTG ACCTCCAGGC TGGAGTTGTT CGCTATCATC ATGATGACAG CGACTCCACC 
AAAGACTTCG TGGTCTTCCG GATATTTGAT GGCCATCACA GCATCCGTCA CAAATTCCCC 
ATCAACGTCT TGCCCAAAGA TGATAGTCCC CCGTTCCTCA TAACCAATGT TGTGATTGAA 
CTGGAGGAGG GGCAGACCAT CCTGATCCAG GGATCCATGC TGCGAGCTTC AGATGTGGAC 
GCCAGTGATG ACTACATCTT CTTCAATATC ACAAAGCCTC CACAGGCTGG GGAGATCATG 
AAGAAGCCAG GGCCAGGACT GATAGGCTAT CCTGTCCATG GCTTCCTTCA GAGGGATTTG 
TTTAATGGAA TCATTTATTA TCGTCATTTT GGTGGAGAAA TCTTTGAAGA TTCTTTTCAA 
TTTGTCCTGT GGGACAGCCA TGAACCTCCA AATCTTTCAG TGCCACAGGT GGCAACAATC 
CATATAACTC CAGTGGATGA CCAGCTTCCA AAAGAGGCTC CTGGAGTTTC TCGGCATTTG 
GTTGTCAAGG AAACTGAGGT GGCCTATATA ACTAAGAAAC AGCTACATTT TATAGATTCA 
GAATCATATG ACAGGGAGCT GGTCTACACA ATAACTACTC CTCCATTTTT CTCCTTCAGC 
CACAGACACT TGGATGCTGG GAAATTATTT ATGGTGGACA GCATACCAAA AGTAGTTAAG 
AATCCTACGG CCCTGGAGCT GAGGTCATTC ACTCAGCATG CTGTGAACTA TATGAAAGTG 
GCCTACATGC CCCCCATGCA AGACATTGGT CCCCATTGCA GAGATGTCCA GTTCACATTT 
TCTGTCAGTA ACCAACATGG CGGTACTTTG CATGGGATCT GCTTTAACAT TACAATCCTC 
CCAGTGGACA ATCAAGTTCC TGAGGCGTTC ACCAACCCTC TGAAAGTGAC TGAGGGAGGT 
CAAAGCATCA TCAGCACAGA GCACATTCTA ATTTCTGATG CAGATACCAA GCTGGACAAT 
ATTGACCTCT CCCTGCGGGA ATTGCCTCTG CACGGAAGGG TGGAGCTGAA TGGATTTCCT 
CTAAATTCAG GGGGCACATT TTCTTGGGGC GATCTCCATA CCTTAAAAGT TAGGTATCAA 
CATGATGGAA CTGAAGTTCT TCAGGATGAC CTACTCTTGG AGGTCACCGA TGGCACAAAT 
TCAGCAGAAT TTGTACTACA TGTTGAGGTA TTCCCTGTCA ACGATGAGCC ACCAGTCTTA 
AAGGCTGACC TCATGCCTGT CATGAATTGC TCAGAGGGAG GAGAGGTGGT CATCACCTCT 
GAATACATTT TTGCTACTGA TGTGGACAGC GATAACTTGA AGTTGATGTT TGTGATTGCT 
CGCGAACCTC AGCATGGGGT GGTGAGGAGA GCTGGAGTCA CAGTGGATCA GTTCTCTCAG 
AGAGATGTTA TCTCAGAGGC CGTGACATAC AAACACACAG GTGGCGAGAT TGGCCTGATG 
CCTTGTTTTG ACACCATTAC ATTGGTGGTT TCGGATGGAG AGGCTGGCCC TTTTGTGAAT 
GGCTGTTGCT ACAATGGACC CAATCCATCT GTTCCACTTC ATGCGTCCTT TCCAGTGTAT 
GATCTCAACA TCACGGTATA CCCAGTAGAC AACCAGCCAC CTTCCATTGC AATAGGTCCC 
GTGTTTGTTG TAGATGAGGG CTGCTCAACA GCCCTTACTG TTAACCATTT GTCCGCCACT 
GACCCTGACA CTGCAGCAGA TGACTTGGAA TTTGTTTTGG TTTCTCCTCC TCAGTTTGGC 
TACCTCGAAA ATATACTCCC TTCTGTGGGT TTTGAAAAAA GCAATATTGG CATAAGTATA 
GATTCATTTC AGTGGAAAGA CATGAACGCT TTTCACATTA ACTATGTGCA GTCCAGGCAT 
CTGAGGATAG AACCAACTGC CGACCAGTTC ACGGTGTACG TCACAGATGG GAAGCATCAC 
TCCTTGGAGA TACCATTTTC TATTATAATC AACCCCACAA ATGATGAAGC TCCTGACTTT 
GTAGTGCAGA ATATTACTGT GTGTGAGGGT CAGATGAAAG AGCTGGACTC TTCCATCATC 
AGCGCTGTGG ACCTGGACAT TCCCCAGGAT GCCCTGCTGT TCAGCATCAC TCAAAAGCCA 
CGCCATGGCC TCCTCATCGA TAGGGGGTTT AGCAAAGACT TCTCTGAGAA TAAGCAGCCA 
GCCAACCCTC ACCAGAAACA TGCACCTGTT CACAGCTTTT CCATGGAACT CCTCAAGACT 
GGAATGAGGT TGACGTACAT GCATGATGAC TCAGAGAGCC TTGCTGATGA TTTTACAATC 
CAATTGTCAG ATGGGAAACA TAAGATACTT AAAACCATTT CAGTAGAGGT CATCCCAGTT 
AATGATGAAA AACCAATGCT GAGCAAGAAG GCTGAAATTG CAATGAATAT GGGTGAAACT 
CGTATTATTT CCAGTGCTAT TCTTTCAGCC ATAGATGAAG ACTCACCCAG GGAGAAGATT 
TACTATGTAT TTGAAAGGCT TCCCCAAAAT GGGCAACTTC AGCTTAAGAT AGGGAGGGAC 
TGGGTTCCTC TCTCCCCTGG CATGAAATGC ACTCAGGAGG AAGTGGATCT GAACTTGCTG 
AGATACACAC ACACCGGGGC AATGGATTCC CAGAATCAAG ATAGCTTCAC CTTCTACCTT 
TGGGATGGCA ACAACAGGTC CCCTGCTCTT GACTGTCAAA TCACCATCAA GGACATGGAA 
AAAGGTGATA TTGTCATCCT TACAAAACCA CTCGTGGTGT CTAAAGGTGA TAGAGGTTTC 
TTAACAACCA CCACGCTCCT GGCTGTGGAC GGAACAGACA AGCCTGAGGA ACTGCTCTAT 
GTCATCACCT CCCCTCCGCG ATATGGCCAG ATCGAATATG TTCACTATCC TGGAGTTCCC 
ATTACAAACT TCAGCCAAAT GGATGTAGTG GGGCAGACAG TTTGCTATGT ACACAAGAGC 
AAGGTGACTG TCTCCAGTGA CAGATTCAGA TTCATCATCA GCAATGGACT GCGGACCGAG 
CACGGGGTGT TTGAGATCAC ACTGGAGACT GTGGACAGAG CCCTGCCTGT GGTAACCAGG 
AACAAGGGGT TGAGACTGGC CCAAGGGGCC GTGGGCCTGC TTTCCCCTGA CCTCCTTCAG 
CTGACCGACC CTGATACACC TGCGGAGAAC CTCACCTTCC TCTTGGTTCA GCTCCCCCAG 
CATGGCCAGC TCTACCTGTG GGGGACAGGG CTACTTCAAC ACAATTTCAC CCAGCAGGAT 
GTGGACAGCA AGAATGTGGC CTATCGGCAC TCAGGAGGGG ACTCCCAGAC TGACTGCTTT 
ACTTTCATGG CCACAGATGG GACAAACCAA GGCTTTATTG TGAATGGGAG AGTGTGGGAA 
GAACCTGTTT TATTCACCAT TCAGGTAGAC CAATTGGACA AAACAGCTCC TCGTATCACA 
CTCTTGCATT CCCCTTCTCA AGTGGGGCTC CTGAAAAATG GCTGCTACGG GATTTACATC 
ACTTCCCGCG TGTTGAAGGC ATCAGACCCT GACACTGAGG ACGATCAGAT CATCTTTAAA 
ATTCTACAAG GCCCAAAACA TGGACATCTG GAGAACACAA CAACAGGTGA ATTTATCCAT 
GAGAAATTTA GCCAAAAGGA CTTAAACAGT AAGACTATTC TTTACATCAT AAACCCATCT 
TTGGAAGTAA ATTCAGATAC CGTGGAATTT CAAATCATGG ACCCCACAGG GAACTCGGCC 
ACTCCTCAAA TTTTGGAACT GAAGTGGTCT CATATTGAAT GGTCACAGAC CGAATATGAA 
GTCTGTGAGA ATGTGGGTTT GTTGCCCTTG GAAATTATCA GAAGGGGATA TTCCATGGAC 
TCGGCCTTTG TGGGTATAAA GGTCAACCAA GTGTCAGCTG CAGTTGGAAA AGATTTCACC 
GTGATTCCAT CTAAACTGAT TCAGTTTGAC CCAGGAATGT CAACTAAGAT GTGGAATATA 
GCAATTACCT ATGACGGATT AGAGGAAGAT GATGAGGTCT TTGAAGTAAT TCTGAACTCC 
CCTGTGAATG CAGTTCTTGG CACAAAGACA AAAGCTGCAG TGAAAATTTT GGACTCAAAA 
GGAGGACAAT GCCATCCTTC ATATTCCTCC AACCAAAGCA AGCACAGCAC ATGGGAGAAG 
GGCATTTGGC ATCTGCTGCC CCCAGGGTCT TCCTCATCCA CCACTTCTGG TTCCTTTCAT 
CTGGAAAGAA GACCTCTTCC ATCTTCCATG CAGCTAGCAG TCATCAGGGG AGACACCCTG 
CGGGGCTTTG ATTCTACAGA TCTTTCTCAA AGGAAGCTTA GGACCCGTGG GAATGGCAAA 
ACAGTTCGTC CATCCTCTGT TTATAGAAAT GGAACAGACA TCATCTATAA TTATCATGGG 
ATAGTTTCCT TGAAACTGGA GGATGACAGT TTCCCAACTC ACAAAAGGAA GGCCAAAGTA 
TCCATCATTA GTCAGCCACA AAAGACAATC AAAGTGGCAG AACTGCCTCA AGCAGATAAG 
GTGGAATCCA CAACTGACTC ACACTTCCCC AGACAGGACC AGTTGCCCTC ATTTCCAAAG 
AACTGCACTC TGGAATTAAA GGGACTCTTC CATTTTGAAG AAGGCATCCA GAAGCTGTAT 
CAGTGCAATG GGATCGCCTG GAAAGCCTGG AGTCCCCAAA CCAAGGATGT GGAAGACAAA 
TCCTGTCCAG CCGGGTGGCA CCAGCACTCA GGCTACTGTC ACATCTTGAT CACAGAGCAG 
AAAGGCACCT GGAATGCGGC TGCCCAAGCT TGCAGGGAAC AATACCTGGG CAACCTTGTA 
ACTGTATTCT CCAGGCAGCA CATGCGGTGG CTCTGGGACA TTGGTGGGAG AAAGTCCTTT 
TGGATAGGTT TGAACGACCA AGTGCATGCT GGCCACTGGG AGTGGATCGG TGGTGAACCT 
GTTGCCTTCA CCAATGGGAG AAGAGGGCCC TCTCAACGCT CCAAGCTTGG AAAGAGCTGT 
GTTTTGGTTC AAAGACAAGG GAAATGGCAA ACAAAAGACT GTAGGAGAGC CAAACCTCAT 
AATTATGTGT GTTCCAGAAA ACTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016869810.1
CDS	852..7418
Translation	MNSLSWGAANAVLLLLLLAWASPTFISINRGVRVMKGHSAFLSGDDLKFAIPKEKDACKVEVVMNEPITQRVGKLTPQVFDCHFLPNEVKYVHNGCPILDEDTVKLRLYRFTERDTFIETFILWVYLLEPDCNIIHMSNNVLEVPEFNGLSQAIDKNLLRFDYDRMASLECTVSLDTARTRLPAHGQMVLGEPRPEEPRGDQPHSFFPESRMMSFNAFLELRAKLKCPGGSCTPGLKKIGSLKVSCEEFLLMGLRYQHLDPPSPNIDYISIQLDLTDTRSKIVYKSESAWLPVYIRAGIPNQIPKAAFMAVFILEVDQFILTSLTTSVLDCEEDETPKPLLVFNITKAPLQGYVTHLLDHTRPISSFTWKDLSDMQIAYQPPNSSHSERRHDEVELEVYDFFFERSAPMTVHISIRTADTNAPRVSWNTGLSLLEGQSRAITWEQFQVVDNDDIGAVRLVTVGGLQHGWLTLRGGKGFLFTVADLQAGVVRYHHDDSDSTKDFVVFRIFDGHHSIRHKFPINVLPKDDSPPFLITNVVIELEEGQTILIQGSMLRASDVDASDDYIFFNITKPPQAGEIMKKPGPGLIGYPVHGFLQRDLFNGIIYYRHFGGEIFEDSFQFVLWDSHEPPNLSVPQVATIHITPVDDQLPKEAPGVSRHLVVKETEVAYITKKQLHFIDSESYDRELVYTITTPPFFSFSHRHLDAGKLFMVDSIPKVVKNPTALELRSFTQHAVNYMKVAYMPPMQDIGPHCRDVQFTFSVSNQHGGTLHGICFNITILPVDNQVPEAFTNPLKVTEGGQSIISTEHILISDADTKLDNIDLSLRELPLHGRVELNGFPLNSGGTFSWGDLHTLKVRYQHDGTEVLQDDLLLEVTDGTNSAEFVLHVEVFPVNDEPPVLKADLMPVMNCSEGGEVVITSEYIFATDVDSDNLKLMFVIAREPQHGVVRRAGVTVDQFSQRDVISEAVTYKHTGGEIGLMPCFDTITLVVSDGEAGPFVNGCCYNGPNPSVPLHASFPVYDLNITVYPVDNQPPSIAIGPVFVVDEGCSTALTVNHLSATDPDTAADDLEFVLVSPPQFGYLENILPSVGFEKSNIGISIDSFQWKDMNAFHINYVQSRHLRIEPTADQFTVYVTDGKHHSLEIPFSIIINPTNDEAPDFVVQNITVCEGQMKELDSSIISAVDLDIPQDALLFSITQKPRHGLLIDRGFSKDFSENKQPANPHQKHAPVHSFSMELLKTGMRLTYMHDDSESLADDFTIQLSDGKHKILKTISVEVIPVNDEKPMLSKKAEIAMNMGETRIISSAILSAIDEDSPREKIYYVFERLPQNGQLQLKIGRDWVPLSPGMKCTQEEVDLNLLRYTHTGAMDSQNQDSFTFYLWDGNNRSPALDCQITIKDMEKGDIVILTKPLVVSKGDRGFLTTTTLLAVDGTDKPEELLYVITSPPRYGQIEYVHYPGVPITNFSQMDVVGQTVCYVHKSKVTVSSDRFRFIISNGLRTEHGVFEITLETVDRALPVVTRNKGLRLAQGAVGLLSPDLLQLTDPDTPAENLTFLLVQLPQHGQLYLWGTGLLQHNFTQQDVDSKNVAYRHSGGDSQTDCFTFMATDGTNQGFIVNGRVWEEPVLFTIQVDQLDKTAPRITLLHSPSQVGLLKNGCYGIYITSRVLKASDPDTEDDQIIFKILQGPKHGHLENTTTGEFIHEKFSQKDLNSKTILYIINPSLEVNSDTVEFQIMDPTGNSATPQILELKWSHIEWSQTEYEVCENVGLLPLEIIRRGYSMDSAFVGIKVNQVSAAVGKDFTVIPSKLIQFDPGMSTKMWNIAITYDGLEEDDEVFEVILNSPVNAVLGTKTKAAVKILDSKGGQCHPSYSSNQSKHSTWEKGIWHLLPPGSSSSTTSGSFHLERRPLPSSMQLAVIRGDTLRGFDSTDLSQRKLRTRGNGKTVRPSSVYRNGTDIIYNYHGIVSLKLEDDSFPTHKRKAKVSIISQPQKTIKVAELPQADKVESTTDSHFPRQDQLPSFPKNCTLELKGLFHFEEGIQKLYQCNGIAWKAWSPQTKDVEDKSCPAGWHQHSGYCHILITEQKGTWNAAAQACREQYLGNLVTVFSRQHMRWLWDIGGRKSFWIGLNDQVHAGHWEWIGGEPVAFTNGRRGPSQRSKLGKSCVLVQRQGKWQTKDCRRAKPHNYVCSRKL

Target ORF information:

RefSeq Version	XM_017014321.2
Organism	Homo sapiens(human)
Definition	Homo sapiens FRAS1 related extracellular matrix 1 (FREM1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017014321.2

ORF Insert Sequence:

ATGAACTCTC TGAGTTGGGG GGCTGCGAAT GCCGTGCTGC TGCTGCTCCT CCTGGCCTGG 
GCCAGCCCCA CCTTCATCAG CATCAACCGC GGGGTGAGGG TGATGAAGGG CCACTCTGCC 
TTCCTGTCAG GAGATGACCT GAAGTTTGCC ATCCCTAAAG AGAAAGATGC CTGCAAAGTG 
GAAGTTGTGA TGAATGAGCC AATAACCCAG AGGGTTGGGA AACTCACTCC ACAGGTCTTT 
GACTGCCATT TCCTTCCCAA CGAAGTCAAG TATGTTCACA ATGGTTGTCC AATTCTTGAT 
GAAGACACAG TGAAGCTCAG ACTTTACAGA TTTACTGAAA GAGATACCTT CATAGAAACT 
TTTATCCTGT GGGTCTATCT CCTGGAACCA GACTGTAACA TCATCCATAT GAGTAACAAT 
GTGCTGGAGG TGCCTGAATT CAATGGCTTG TCCCAAGCGA TTGATAAAAA TCTGCTCAGA 
TTCGATTATG ATAGGATGGC TAGCCTGGAA TGTACCGTCA GCCTGGACAC TGCGAGAACT 
CGGCTGCCAG CCCATGGCCA GATGGTTCTC GGGGAGCCTC GACCAGAAGA ACCTCGTGGA 
GATCAGCCAC ACAGTTTTTT CCCAGAGTCC CGTATGATGT CTTTTAATGC CTTTCTGGAA 
CTGAGAGCAA AACTGAAGTG TCCAGGTGGG AGCTGTACCC CAGGATTAAA GAAAATAGGA 
AGCCTCAAAG TGAGCTGTGA GGAGTTCCTG CTGATGGGCC TTCGTTATCA GCATCTGGAT 
CCCCCTTCAC CCAACATTGA TTATATCTCC ATCCAACTGG ACCTCACAGA TACCAGGAGC 
AAAATTGTGT ACAAGTCAGA GAGTGCGTGG CTGCCTGTCT ATATCAGAGC TGGAATTCCG 
AATCAGATTC CAAAGGCTGC ATTCATGGCC GTGTTTATTC TGGAAGTGGA TCAGTTCATC 
CTGACCTCCT TGACTACATC AGTTCTGGAC TGTGAAGAAG ATGAGACCCC TAAACCCTTG 
CTGGTGTTCA ACATTACTAA AGCCCCGCTC CAGGGCTATG TGACTCACCT GTTGGATCAC 
ACCAGACCAA TCTCCTCATT CACCTGGAAA GATCTCAGTG ACATGCAGAT CGCCTATCAG 
CCACCAAACA GCAGCCATTC TGAGAGGAGA CATGATGAGG TAGAATTGGA GGTATACGAC 
TTCTTCTTTG AAAGGAGTGC ACCTATGACA GTCCACATCT CCATCAGAAC AGCAGATACA 
AATGCCCCCC GTGTATCCTG GAATACAGGT CTGAGTCTCC TTGAGGGGCA GTCTCGAGCC 
ATCACTTGGG AACAGTTTCA GGTTGTCGAC AATGACGACA TTGGTGCTGT CCGGCTAGTC 
ACCGTTGGTG GCCTGCAGCA TGGATGGCTG ACTTTAAGAG GGGGGAAAGG GTTTCTCTTC 
ACCGTGGCTG ACCTCCAGGC TGGAGTTGTT CGCTATCATC ATGATGACAG CGACTCCACC 
AAAGACTTCG TGGTCTTCCG GATATTTGAT GGCCATCACA GCATCCGTCA CAAATTCCCC 
ATCAACGTCT TGCCCAAAGA TGATAGTCCC CCGTTCCTCA TAACCAATGT TGTGATTGAA 
CTGGAGGAGG GGCAGACCAT CCTGATCCAG GGATCCATGC TGCGAGCTTC AGATGTGGAC 
GCCAGTGATG ACTACATCTT CTTCAATATC ACAAAGCCTC CACAGGCTGG GGAGATCATG 
AAGAAGCCAG GGCCAGGACT GATAGGCTAT CCTGTCCATG GCTTCCTTCA GAGGGATTTG 
TTTAATGGAA TCATTTATTA TCGTCATTTT GGTGGAGAAA TCTTTGAAGA TTCTTTTCAA 
TTTGTCCTGT GGGACAGCCA TGAACCTCCA AATCTTTCAG TGCCACAGGT GGCAACAATC 
CATATAACTC CAGTGGATGA CCAGCTTCCA AAAGAGGCTC CTGGAGTTTC TCGGCATTTG 
GTTGTCAAGG AAACTGAGGT GGCCTATATA ACTAAGAAAC AGCTACATTT TATAGATTCA 
GAATCATATG ACAGGGAGCT GGTCTACACA ATAACTACTC CTCCATTTTT CTCCTTCAGC 
CACAGACACT TGGATGCTGG GAAATTATTT ATGGTGGACA GCATACCAAA AGTAGTTAAG 
AATCCTACGG CCCTGGAGCT GAGGTCATTC ACTCAGCATG CTGTGAACTA TATGAAAGTG 
GCCTACATGC CCCCCATGCA AGACATTGGT CCCCATTGCA GAGATGTCCA GTTCACATTT 
TCTGTCAGTA ACCAACATGG CGGTACTTTG CATGGGATCT GCTTTAACAT TACAATCCTC 
CCAGTGGACA ATCAAGTTCC TGAGGCGTTC ACCAACCCTC TGAAAGTGAC TGAGGGAGGT 
CAAAGCATCA TCAGCACAGA GCACATTCTA ATTTCTGATG CAGATACCAA GCTGGACAAT 
ATTGACCTCT CCCTGCGGGA ATTGCCTCTG CACGGAAGGG TGGAGCTGAA TGGATTTCCT 
CTAAATTCAG GGGGCACATT TTCTTGGGGC GATCTCCATA CCTTAAAAGT TAGGTATCAA 
CATGATGGAA CTGAAGTTCT TCAGGATGAC CTACTCTTGG AGGTCACCGA TGGCACAAAT 
TCAGCAGAAT TTGTACTACA TGTTGAGGTA TTCCCTGTCA ACGATGAGCC ACCAGTCTTA 
AAGGCTGACC TCATGCCTGT CATGAATTGC TCAGAGGGAG GAGAGGTGGT CATCACCTCT 
GAATACATTT TTGCTACTGA TGTGGACAGC GATAACTTGA AGTTGATGTT TGTGATTGCT 
CGCGAACCTC AGCATGGGGT GGTGAGGAGA GCTGGAGTCA CAGTGGATCA GTTCTCTCAG 
AGAGATGTTA TCTCAGAGGC CGTGACATAC AAACACACAG GTGGCGAGAT TGGCCTGATG 
CCTTGTTTTG ACACCATTAC ATTGGTGGTT TCGGATGGAG AGGCTGGCCC TTTTGTGAAT 
GGCTGTTGCT ACAATGGACC CAATCCATCT GTTCCACTTC ATGCGTCCTT TCCAGTGTAT 
GATCTCAACA TCACGGTATA CCCAGTAGAC AACCAGCCAC CTTCCATTGC AATAGGTCCC 
GTGTTTGTTG TAGATGAGGG CTGCTCAACA GCCCTTACTG TTAACCATTT GTCCGCCACT 
GACCCTGACA CTGCAGCAGA TGACTTGGAA TTTGTTTTGG TTTCTCCTCC TCAGTTTGGC 
TACCTCGAAA ATATACTCCC TTCTGTGGGT TTTGAAAAAA GCAATATTGG CATAAGTATA 
GATTCATTTC AGTGGAAAGA CATGAACGCT TTTCACATTA ACTATGTGCA GTCCAGGCAT 
CTGAGGATAG AACCAACTGC CGACCAGTTC ACGGTGTACG TCACAGATGG GAAGCATCAC 
TCCTTGGAGA TACCATTTTC TATTATAATC AACCCCACAA ATGATGAAGC TCCTGACTTT 
GTAGTGCAGA ATATTACTGT GTGTGAGGGT CAGATGAAAG AGCTGGACTC TTCCATCATC 
AGCGCTGTGG ACCTGGACAT TCCCCAGGAT GCCCTGCTGT TCAGCATCAC TCAAAAGCCA 
CGCCATGGCC TCCTCATCGA TAGGGGGTTT AGCAAAGACT TCTCTGAGAA TAAGCAGCCA 
GCCAACCCTC ACCAGAAACA TGCACCTGTT CACAGCTTTT CCATGGAACT CCTCAAGACT 
GGAATGAGGT TGACGTACAT GCATGATGAC TCAGAGAGCC TTGCTGATGA TTTTACAATC 
CAATTGTCAG ATGGGAAACA TAAGATACTT AAAACCATTT CAGTAGAGGT CATCCCAGTT 
AATGATGAAA AACCAATGCT GAGCAAGAAG GCTGAAATTG CAATGAATAT GGGTGAAACT 
CGTATTATTT CCAGTGCTAT TCTTTCAGCC ATAGATGAAG ACTCACCCAG GGAGAAGATT 
TACTATGTAT TTGAAAGGCT TCCCCAAAAT GGGCAACTTC AGCTTAAGAT AGGGAGGGAC 
TGGGTTCCTC TCTCCCCTGG CATGAAATGC ACTCAGGAGG AAGTGGATCT GAACTTGCTG 
AGATACACAC ACACCGGGGC AATGGATTCC CAGAATCAAG ATAGCTTCAC CTTCTACCTT 
TGGGATGGCA ACAACAGGTC CCCTGCTCTT GACTGTCAAA TCACCATCAA GGACATGGAA 
AAAGGTGATA TTGTCATCCT TACAAAACCA CTCGTGGTGT CTAAAGGTGA TAGAGGTTTC 
TTAACAACCA CCACGCTCCT GGCTGTGGAC GGAACAGACA AGCCTGAGGA ACTGCTCTAT 
GTCATCACCT CCCCTCCGCG ATATGGCCAG ATCGAATATG TTCACTATCC TGGAGTTCCC 
ATTACAAACT TCAGCCAAAT GGATGTAGTG GGGCAGACAG TTTGCTATGT ACACAAGAGC 
AAGGTGACTG TCTCCAGTGA CAGATTCAGA TTCATCATCA GCAATGGACT GCGGACCGAG 
CACGGGGTGT TTGAGATCAC ACTGGAGACT GTGGACAGAG CCCTGCCTGT GGTAACCAGG 
AACAAGGGGT TGAGACTGGC CCAAGGGGCC GTGGGCCTGC TTTCCCCTGA CCTCCTTCAG 
CTGACCGACC CTGATACACC TGCGGAGAAC CTCACCTTCC TCTTGGTTCA GCTCCCCCAG 
CATGGCCAGC TCTACCTGTG GGGGACAGGG CTACTTCAAC ACAATTTCAC CCAGCAGGAT 
GTGGACAGCA AGAATGTGGC CTATCGGCAC TCAGGAGGGG ACTCCCAGAC TGACTGCTTT 
ACTTTCATGG CCACAGATGG GACAAACCAA GGCTTTATTG TGAATGGGAG AGTGTGGGAA 
GAACCTGTTT TATTCACCAT TCAGGTAGAC CAATTGGACA AAACAGCTCC TCGTATCACA 
CTCTTGCATT CCCCTTCTCA AGTGGGGCTC CTGAAAAATG GCTGCTACGG GATTTACATC 
ACTTCCCGCG TGTTGAAGGC ATCAGACCCT GACACTGAGG ACGATCAGAT CATCTTTAAA 
ATTCTACAAG GCCCAAAACA TGGACATCTG GAGAACACAA CAACAGGTGA ATTTATCCAT 
GAGAAATTTA GCCAAAAGGA CTTAAACAGT AAGACTATTC TTTACATCAT AAACCCATCT 
TTGGAAGTAA ATTCAGATAC CGTGGAATTT CAAATCATGG ACCCCACAGG GAACTCGGCC 
ACTCCTCAAA TTTTGGAACT GAAGTGGTCT CATATTGAAT GGTCACAGAC CGAATATGAA 
GTCTGTGAGA ATGTGGGTTT GTTGCCCTTG GAAATTATCA GAAGGGGATA TTCCATGGAC 
TCGGCCTTTG TGGGTATAAA GGTCAACCAA GTGTCAGCTG CAGTTGGAAA AGATTTCACC 
GTGATTCCAT CTAAACTGAT TCAGTTTGAC CCAGGAATGT CAACTAAGAT GTGGAATATA 
GCAATTACCT ATGACGGATT AGAGGAAGAT GATGAGGTCT TTGAAGTAAT TCTGAACTCC 
CCTGTGAATG CAGTTCTTGG CACAAAGACA AAAGCTGCAG TGAAAATTTT GGACTCAAAA 
GGAGGACAAT GCCATCCTTC ATATTCCTCC AACCAAAGCA AGCACAGCAC ATGGGAGAAG 
GGCATTTGGC ATCTGCTGCC CCCAGGGTCT TCCTCATCCA CCACTTCTGG TTCCTTTCAT 
CTGGAAAGAA GACCTCTTCC ATCTTCCATG CAGCTAGCAG TCATCAGGGG AGACACCCTG 
CGGGGCTTTG ATTCTACAGA TCTTTCTCAA AGGAAGCTTA GGACCCGTGG GAATGGCAAA 
ACAGTTCGTC CATCCTCTGT TTATAGAAAT GGAACAGACA TCATCTATAA TTATCATGGG 
ATAGTTTCCT TGAAACTGGA GGATGACAGT TTCCCAACTC ACAAAAGGAA GGCCAAAGTA 
TCCATCATTA GTCAGCCACA AAAGACAATC AAAGTGGCAG AACTGCCTCA AGCAGATAAG 
GTGGAATCCA CAACTGACTC ACACTTCCCC AGACAGGACC AGTTGCCCTC ATTTCCAAAG 
AACTGCACTC TGGAATTAAA GGGACTCTTC CATTTTGAAG AAGGCATCCA GAAGCTGTAT 
CAGTGCAATG GGATCGCCTG GAAAGCCTGG AGTCCCCAAA CCAAGGATGT GGAAGACAAA 
TCCTGTCCAG CCGGGTGGCA CCAGCACTCA GGCTACTGTC ACATCTTGAT CACAGAGCAG 
AAAGGCACCT GGAATGCGGC TGCCCAAGCT TGCAGGGAAC AATACCTGGG CAACCTTGTA 
ACTGTATTCT CCAGGCAGCA CATGCGGTGG CTCTGGGACA TTGGTGGGAG AAAGTCCTTT 
TGGATAGGTT TGAACGACCA AGTGCATGCT GGCCACTGGG AGTGGATCGG TGGTGAACCT 
GTTGCCTTCA CCAATGGGAG AAGAGGGCCC TCTCAACGCT CCAAGCTTGG AAAGAGCTGT 
GTTTTGGTTC AAAGACAAGG GAAATGGCAA ACAAAAGACT GTAGGAGAGC CAAACCTCAT 
AATTATGTGT GTTCCAGAAA ACTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FREM1 ( XM_017014323.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016869812.1 Homo sapiens FRAS1 related extracellular matrix 1 (FREM1), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OHu98459
Clone ID Related Accession (Same CDS sequence)	XM_017014320.1 , XM_017014318.1 , XM_017014316.1 , XM_017014316.2 , XM_017014317.1 , XM_017014319.2 , XM_017014321.2 , XM_017014323.1 , XM_017014322.1 , XM_017014321.1 , XM_017014320.2 , XM_017014319.1
Accession Version	XM_017014323.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6567bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	FRAS1-related extracellular matrix protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACTCTC TGAGTTGGGG GGCTGCGAAT GCCGTGCTGC TGCTGCTCCT CCTGGCCTGG 
GCCAGCCCCA CCTTCATCAG CATCAACCGC GGGGTGAGGG TGATGAAGGG CCACTCTGCC 
TTCCTGTCAG GAGATGACCT GAAGTTTGCC ATCCCTAAAG AGAAAGATGC CTGCAAAGTG 
GAAGTTGTGA TGAATGAGCC AATAACCCAG AGGGTTGGGA AACTCACTCC ACAGGTCTTT 
GACTGCCATT TCCTTCCCAA CGAAGTCAAG TATGTTCACA ATGGTTGTCC AATTCTTGAT 
GAAGACACAG TGAAGCTCAG ACTTTACAGA TTTACTGAAA GAGATACCTT CATAGAAACT 
TTTATCCTGT GGGTCTATCT CCTGGAACCA GACTGTAACA TCATCCATAT GAGTAACAAT 
GTGCTGGAGG TGCCTGAATT CAATGGCTTG TCCCAAGCGA TTGATAAAAA TCTGCTCAGA 
TTCGATTATG ATAGGATGGC TAGCCTGGAA TGTACCGTCA GCCTGGACAC TGCGAGAACT 
CGGCTGCCAG CCCATGGCCA GATGGTTCTC GGGGAGCCTC GACCAGAAGA ACCTCGTGGA 
GATCAGCCAC ACAGTTTTTT CCCAGAGTCC CGTATGATGT CTTTTAATGC CTTTCTGGAA 
CTGAGAGCAA AACTGAAGTG TCCAGGTGGG AGCTGTACCC CAGGATTAAA GAAAATAGGA 
AGCCTCAAAG TGAGCTGTGA GGAGTTCCTG CTGATGGGCC TTCGTTATCA GCATCTGGAT 
CCCCCTTCAC CCAACATTGA TTATATCTCC ATCCAACTGG ACCTCACAGA TACCAGGAGC 
AAAATTGTGT ACAAGTCAGA GAGTGCGTGG CTGCCTGTCT ATATCAGAGC TGGAATTCCG 
AATCAGATTC CAAAGGCTGC ATTCATGGCC GTGTTTATTC TGGAAGTGGA TCAGTTCATC 
CTGACCTCCT TGACTACATC AGTTCTGGAC TGTGAAGAAG ATGAGACCCC TAAACCCTTG 
CTGGTGTTCA ACATTACTAA AGCCCCGCTC CAGGGCTATG TGACTCACCT GTTGGATCAC 
ACCAGACCAA TCTCCTCATT CACCTGGAAA GATCTCAGTG ACATGCAGAT CGCCTATCAG 
CCACCAAACA GCAGCCATTC TGAGAGGAGA CATGATGAGG TAGAATTGGA GGTATACGAC 
TTCTTCTTTG AAAGGAGTGC ACCTATGACA GTCCACATCT CCATCAGAAC AGCAGATACA 
AATGCCCCCC GTGTATCCTG GAATACAGGT CTGAGTCTCC TTGAGGGGCA GTCTCGAGCC 
ATCACTTGGG AACAGTTTCA GGTTGTCGAC AATGACGACA TTGGTGCTGT CCGGCTAGTC 
ACCGTTGGTG GCCTGCAGCA TGGATGGCTG ACTTTAAGAG GGGGGAAAGG GTTTCTCTTC 
ACCGTGGCTG ACCTCCAGGC TGGAGTTGTT CGCTATCATC ATGATGACAG CGACTCCACC 
AAAGACTTCG TGGTCTTCCG GATATTTGAT GGCCATCACA GCATCCGTCA CAAATTCCCC 
ATCAACGTCT TGCCCAAAGA TGATAGTCCC CCGTTCCTCA TAACCAATGT TGTGATTGAA 
CTGGAGGAGG GGCAGACCAT CCTGATCCAG GGATCCATGC TGCGAGCTTC AGATGTGGAC 
GCCAGTGATG ACTACATCTT CTTCAATATC ACAAAGCCTC CACAGGCTGG GGAGATCATG 
AAGAAGCCAG GGCCAGGACT GATAGGCTAT CCTGTCCATG GCTTCCTTCA GAGGGATTTG 
TTTAATGGAA TCATTTATTA TCGTCATTTT GGTGGAGAAA TCTTTGAAGA TTCTTTTCAA 
TTTGTCCTGT GGGACAGCCA TGAACCTCCA AATCTTTCAG TGCCACAGGT GGCAACAATC 
CATATAACTC CAGTGGATGA CCAGCTTCCA AAAGAGGCTC CTGGAGTTTC TCGGCATTTG 
GTTGTCAAGG AAACTGAGGT GGCCTATATA ACTAAGAAAC AGCTACATTT TATAGATTCA 
GAATCATATG ACAGGGAGCT GGTCTACACA ATAACTACTC CTCCATTTTT CTCCTTCAGC 
CACAGACACT TGGATGCTGG GAAATTATTT ATGGTGGACA GCATACCAAA AGTAGTTAAG 
AATCCTACGG CCCTGGAGCT GAGGTCATTC ACTCAGCATG CTGTGAACTA TATGAAAGTG 
GCCTACATGC CCCCCATGCA AGACATTGGT CCCCATTGCA GAGATGTCCA GTTCACATTT 
TCTGTCAGTA ACCAACATGG CGGTACTTTG CATGGGATCT GCTTTAACAT TACAATCCTC 
CCAGTGGACA ATCAAGTTCC TGAGGCGTTC ACCAACCCTC TGAAAGTGAC TGAGGGAGGT 
CAAAGCATCA TCAGCACAGA GCACATTCTA ATTTCTGATG CAGATACCAA GCTGGACAAT 
ATTGACCTCT CCCTGCGGGA ATTGCCTCTG CACGGAAGGG TGGAGCTGAA TGGATTTCCT 
CTAAATTCAG GGGGCACATT TTCTTGGGGC GATCTCCATA CCTTAAAAGT TAGGTATCAA 
CATGATGGAA CTGAAGTTCT TCAGGATGAC CTACTCTTGG AGGTCACCGA TGGCACAAAT 
TCAGCAGAAT TTGTACTACA TGTTGAGGTA TTCCCTGTCA ACGATGAGCC ACCAGTCTTA 
AAGGCTGACC TCATGCCTGT CATGAATTGC TCAGAGGGAG GAGAGGTGGT CATCACCTCT 
GAATACATTT TTGCTACTGA TGTGGACAGC GATAACTTGA AGTTGATGTT TGTGATTGCT 
CGCGAACCTC AGCATGGGGT GGTGAGGAGA GCTGGAGTCA CAGTGGATCA GTTCTCTCAG 
AGAGATGTTA TCTCAGAGGC CGTGACATAC AAACACACAG GTGGCGAGAT TGGCCTGATG 
CCTTGTTTTG ACACCATTAC ATTGGTGGTT TCGGATGGAG AGGCTGGCCC TTTTGTGAAT 
GGCTGTTGCT ACAATGGACC CAATCCATCT GTTCCACTTC ATGCGTCCTT TCCAGTGTAT 
GATCTCAACA TCACGGTATA CCCAGTAGAC AACCAGCCAC CTTCCATTGC AATAGGTCCC 
GTGTTTGTTG TAGATGAGGG CTGCTCAACA GCCCTTACTG TTAACCATTT GTCCGCCACT 
GACCCTGACA CTGCAGCAGA TGACTTGGAA TTTGTTTTGG TTTCTCCTCC TCAGTTTGGC 
TACCTCGAAA ATATACTCCC TTCTGTGGGT TTTGAAAAAA GCAATATTGG CATAAGTATA 
GATTCATTTC AGTGGAAAGA CATGAACGCT TTTCACATTA ACTATGTGCA GTCCAGGCAT 
CTGAGGATAG AACCAACTGC CGACCAGTTC ACGGTGTACG TCACAGATGG GAAGCATCAC 
TCCTTGGAGA TACCATTTTC TATTATAATC AACCCCACAA ATGATGAAGC TCCTGACTTT 
GTAGTGCAGA ATATTACTGT GTGTGAGGGT CAGATGAAAG AGCTGGACTC TTCCATCATC 
AGCGCTGTGG ACCTGGACAT TCCCCAGGAT GCCCTGCTGT TCAGCATCAC TCAAAAGCCA 
CGCCATGGCC TCCTCATCGA TAGGGGGTTT AGCAAAGACT TCTCTGAGAA TAAGCAGCCA 
GCCAACCCTC ACCAGAAACA TGCACCTGTT CACAGCTTTT CCATGGAACT CCTCAAGACT 
GGAATGAGGT TGACGTACAT GCATGATGAC TCAGAGAGCC TTGCTGATGA TTTTACAATC 
CAATTGTCAG ATGGGAAACA TAAGATACTT AAAACCATTT CAGTAGAGGT CATCCCAGTT 
AATGATGAAA AACCAATGCT GAGCAAGAAG GCTGAAATTG CAATGAATAT GGGTGAAACT 
CGTATTATTT CCAGTGCTAT TCTTTCAGCC ATAGATGAAG ACTCACCCAG GGAGAAGATT 
TACTATGTAT TTGAAAGGCT TCCCCAAAAT GGGCAACTTC AGCTTAAGAT AGGGAGGGAC 
TGGGTTCCTC TCTCCCCTGG CATGAAATGC ACTCAGGAGG AAGTGGATCT GAACTTGCTG 
AGATACACAC ACACCGGGGC AATGGATTCC CAGAATCAAG ATAGCTTCAC CTTCTACCTT 
TGGGATGGCA ACAACAGGTC CCCTGCTCTT GACTGTCAAA TCACCATCAA GGACATGGAA 
AAAGGTGATA TTGTCATCCT TACAAAACCA CTCGTGGTGT CTAAAGGTGA TAGAGGTTTC 
TTAACAACCA CCACGCTCCT GGCTGTGGAC GGAACAGACA AGCCTGAGGA ACTGCTCTAT 
GTCATCACCT CCCCTCCGCG ATATGGCCAG ATCGAATATG TTCACTATCC TGGAGTTCCC 
ATTACAAACT TCAGCCAAAT GGATGTAGTG GGGCAGACAG TTTGCTATGT ACACAAGAGC 
AAGGTGACTG TCTCCAGTGA CAGATTCAGA TTCATCATCA GCAATGGACT GCGGACCGAG 
CACGGGGTGT TTGAGATCAC ACTGGAGACT GTGGACAGAG CCCTGCCTGT GGTAACCAGG 
AACAAGGGGT TGAGACTGGC CCAAGGGGCC GTGGGCCTGC TTTCCCCTGA CCTCCTTCAG 
CTGACCGACC CTGATACACC TGCGGAGAAC CTCACCTTCC TCTTGGTTCA GCTCCCCCAG 
CATGGCCAGC TCTACCTGTG GGGGACAGGG CTACTTCAAC ACAATTTCAC CCAGCAGGAT 
GTGGACAGCA AGAATGTGGC CTATCGGCAC TCAGGAGGGG ACTCCCAGAC TGACTGCTTT 
ACTTTCATGG CCACAGATGG GACAAACCAA GGCTTTATTG TGAATGGGAG AGTGTGGGAA 
GAACCTGTTT TATTCACCAT TCAGGTAGAC CAATTGGACA AAACAGCTCC TCGTATCACA 
CTCTTGCATT CCCCTTCTCA AGTGGGGCTC CTGAAAAATG GCTGCTACGG GATTTACATC 
ACTTCCCGCG TGTTGAAGGC ATCAGACCCT GACACTGAGG ACGATCAGAT CATCTTTAAA 
ATTCTACAAG GCCCAAAACA TGGACATCTG GAGAACACAA CAACAGGTGA ATTTATCCAT 
GAGAAATTTA GCCAAAAGGA CTTAAACAGT AAGACTATTC TTTACATCAT AAACCCATCT 
TTGGAAGTAA ATTCAGATAC CGTGGAATTT CAAATCATGG ACCCCACAGG GAACTCGGCC 
ACTCCTCAAA TTTTGGAACT GAAGTGGTCT CATATTGAAT GGTCACAGAC CGAATATGAA 
GTCTGTGAGA ATGTGGGTTT GTTGCCCTTG GAAATTATCA GAAGGGGATA TTCCATGGAC 
TCGGCCTTTG TGGGTATAAA GGTCAACCAA GTGTCAGCTG CAGTTGGAAA AGATTTCACC 
GTGATTCCAT CTAAACTGAT TCAGTTTGAC CCAGGAATGT CAACTAAGAT GTGGAATATA 
GCAATTACCT ATGACGGATT AGAGGAAGAT GATGAGGTCT TTGAAGTAAT TCTGAACTCC 
CCTGTGAATG CAGTTCTTGG CACAAAGACA AAAGCTGCAG TGAAAATTTT GGACTCAAAA 
GGAGGACAAT GCCATCCTTC ATATTCCTCC AACCAAAGCA AGCACAGCAC ATGGGAGAAG 
GGCATTTGGC ATCTGCTGCC CCCAGGGTCT TCCTCATCCA CCACTTCTGG TTCCTTTCAT 
CTGGAAAGAA GACCTCTTCC ATCTTCCATG CAGCTAGCAG TCATCAGGGG AGACACCCTG 
CGGGGCTTTG ATTCTACAGA TCTTTCTCAA AGGAAGCTTA GGACCCGTGG GAATGGCAAA 
ACAGTTCGTC CATCCTCTGT TTATAGAAAT GGAACAGACA TCATCTATAA TTATCATGGG 
ATAGTTTCCT TGAAACTGGA GGATGACAGT TTCCCAACTC ACAAAAGGAA GGCCAAAGTA 
TCCATCATTA GTCAGCCACA AAAGACAATC AAAGTGGCAG AACTGCCTCA AGCAGATAAG 
GTGGAATCCA CAACTGACTC ACACTTCCCC AGACAGGACC AGTTGCCCTC ATTTCCAAAG 
AACTGCACTC TGGAATTAAA GGGACTCTTC CATTTTGAAG AAGGCATCCA GAAGCTGTAT 
CAGTGCAATG GGATCGCCTG GAAAGCCTGG AGTCCCCAAA CCAAGGATGT GGAAGACAAA 
TCCTGTCCAG CCGGGTGGCA CCAGCACTCA GGCTACTGTC ACATCTTGAT CACAGAGCAG 
AAAGGCACCT GGAATGCGGC TGCCCAAGCT TGCAGGGAAC AATACCTGGG CAACCTTGTA 
ACTGTATTCT CCAGGCAGCA CATGCGGTGG CTCTGGGACA TTGGTGGGAG AAAGTCCTTT 
TGGATAGGTT TGAACGACCA AGTGCATGCT GGCCACTGGG AGTGGATCGG TGGTGAACCT 
GTTGCCTTCA CCAATGGGAG AAGAGGGCCC TCTCAACGCT CCAAGCTTGG AAAGAGCTGT 
GTTTTGGTTC AAAGACAAGG GAAATGGCAA ACAAAAGACT GTAGGAGAGC CAAACCTCAT 
AATTATGTGT GTTCCAGAAA ACTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016869812.1
CDS	324..6890
Translation	MNSLSWGAANAVLLLLLLAWASPTFISINRGVRVMKGHSAFLSGDDLKFAIPKEKDACKVEVVMNEPITQRVGKLTPQVFDCHFLPNEVKYVHNGCPILDEDTVKLRLYRFTERDTFIETFILWVYLLEPDCNIIHMSNNVLEVPEFNGLSQAIDKNLLRFDYDRMASLECTVSLDTARTRLPAHGQMVLGEPRPEEPRGDQPHSFFPESRMMSFNAFLELRAKLKCPGGSCTPGLKKIGSLKVSCEEFLLMGLRYQHLDPPSPNIDYISIQLDLTDTRSKIVYKSESAWLPVYIRAGIPNQIPKAAFMAVFILEVDQFILTSLTTSVLDCEEDETPKPLLVFNITKAPLQGYVTHLLDHTRPISSFTWKDLSDMQIAYQPPNSSHSERRHDEVELEVYDFFFERSAPMTVHISIRTADTNAPRVSWNTGLSLLEGQSRAITWEQFQVVDNDDIGAVRLVTVGGLQHGWLTLRGGKGFLFTVADLQAGVVRYHHDDSDSTKDFVVFRIFDGHHSIRHKFPINVLPKDDSPPFLITNVVIELEEGQTILIQGSMLRASDVDASDDYIFFNITKPPQAGEIMKKPGPGLIGYPVHGFLQRDLFNGIIYYRHFGGEIFEDSFQFVLWDSHEPPNLSVPQVATIHITPVDDQLPKEAPGVSRHLVVKETEVAYITKKQLHFIDSESYDRELVYTITTPPFFSFSHRHLDAGKLFMVDSIPKVVKNPTALELRSFTQHAVNYMKVAYMPPMQDIGPHCRDVQFTFSVSNQHGGTLHGICFNITILPVDNQVPEAFTNPLKVTEGGQSIISTEHILISDADTKLDNIDLSLRELPLHGRVELNGFPLNSGGTFSWGDLHTLKVRYQHDGTEVLQDDLLLEVTDGTNSAEFVLHVEVFPVNDEPPVLKADLMPVMNCSEGGEVVITSEYIFATDVDSDNLKLMFVIAREPQHGVVRRAGVTVDQFSQRDVISEAVTYKHTGGEIGLMPCFDTITLVVSDGEAGPFVNGCCYNGPNPSVPLHASFPVYDLNITVYPVDNQPPSIAIGPVFVVDEGCSTALTVNHLSATDPDTAADDLEFVLVSPPQFGYLENILPSVGFEKSNIGISIDSFQWKDMNAFHINYVQSRHLRIEPTADQFTVYVTDGKHHSLEIPFSIIINPTNDEAPDFVVQNITVCEGQMKELDSSIISAVDLDIPQDALLFSITQKPRHGLLIDRGFSKDFSENKQPANPHQKHAPVHSFSMELLKTGMRLTYMHDDSESLADDFTIQLSDGKHKILKTISVEVIPVNDEKPMLSKKAEIAMNMGETRIISSAILSAIDEDSPREKIYYVFERLPQNGQLQLKIGRDWVPLSPGMKCTQEEVDLNLLRYTHTGAMDSQNQDSFTFYLWDGNNRSPALDCQITIKDMEKGDIVILTKPLVVSKGDRGFLTTTTLLAVDGTDKPEELLYVITSPPRYGQIEYVHYPGVPITNFSQMDVVGQTVCYVHKSKVTVSSDRFRFIISNGLRTEHGVFEITLETVDRALPVVTRNKGLRLAQGAVGLLSPDLLQLTDPDTPAENLTFLLVQLPQHGQLYLWGTGLLQHNFTQQDVDSKNVAYRHSGGDSQTDCFTFMATDGTNQGFIVNGRVWEEPVLFTIQVDQLDKTAPRITLLHSPSQVGLLKNGCYGIYITSRVLKASDPDTEDDQIIFKILQGPKHGHLENTTTGEFIHEKFSQKDLNSKTILYIINPSLEVNSDTVEFQIMDPTGNSATPQILELKWSHIEWSQTEYEVCENVGLLPLEIIRRGYSMDSAFVGIKVNQVSAAVGKDFTVIPSKLIQFDPGMSTKMWNIAITYDGLEEDDEVFEVILNSPVNAVLGTKTKAAVKILDSKGGQCHPSYSSNQSKHSTWEKGIWHLLPPGSSSSTTSGSFHLERRPLPSSMQLAVIRGDTLRGFDSTDLSQRKLRTRGNGKTVRPSSVYRNGTDIIYNYHGIVSLKLEDDSFPTHKRKAKVSIISQPQKTIKVAELPQADKVESTTDSHFPRQDQLPSFPKNCTLELKGLFHFEEGIQKLYQCNGIAWKAWSPQTKDVEDKSCPAGWHQHSGYCHILITEQKGTWNAAAQACREQYLGNLVTVFSRQHMRWLWDIGGRKSFWIGLNDQVHAGHWEWIGGEPVAFTNGRRGPSQRSKLGKSCVLVQRQGKWQTKDCRRAKPHNYVCSRKL

Target ORF information:

RefSeq Version	XM_017014323.1
Organism	Homo sapiens(human)
Definition	Homo sapiens FRAS1 related extracellular matrix 1 (FREM1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017014323.1

ORF Insert Sequence:

ATGAACTCTC TGAGTTGGGG GGCTGCGAAT GCCGTGCTGC TGCTGCTCCT CCTGGCCTGG 
GCCAGCCCCA CCTTCATCAG CATCAACCGC GGGGTGAGGG TGATGAAGGG CCACTCTGCC 
TTCCTGTCAG GAGATGACCT GAAGTTTGCC ATCCCTAAAG AGAAAGATGC CTGCAAAGTG 
GAAGTTGTGA TGAATGAGCC AATAACCCAG AGGGTTGGGA AACTCACTCC ACAGGTCTTT 
GACTGCCATT TCCTTCCCAA CGAAGTCAAG TATGTTCACA ATGGTTGTCC AATTCTTGAT 
GAAGACACAG TGAAGCTCAG ACTTTACAGA TTTACTGAAA GAGATACCTT CATAGAAACT 
TTTATCCTGT GGGTCTATCT CCTGGAACCA GACTGTAACA TCATCCATAT GAGTAACAAT 
GTGCTGGAGG TGCCTGAATT CAATGGCTTG TCCCAAGCGA TTGATAAAAA TCTGCTCAGA 
TTCGATTATG ATAGGATGGC TAGCCTGGAA TGTACCGTCA GCCTGGACAC TGCGAGAACT 
CGGCTGCCAG CCCATGGCCA GATGGTTCTC GGGGAGCCTC GACCAGAAGA ACCTCGTGGA 
GATCAGCCAC ACAGTTTTTT CCCAGAGTCC CGTATGATGT CTTTTAATGC CTTTCTGGAA 
CTGAGAGCAA AACTGAAGTG TCCAGGTGGG AGCTGTACCC CAGGATTAAA GAAAATAGGA 
AGCCTCAAAG TGAGCTGTGA GGAGTTCCTG CTGATGGGCC TTCGTTATCA GCATCTGGAT 
CCCCCTTCAC CCAACATTGA TTATATCTCC ATCCAACTGG ACCTCACAGA TACCAGGAGC 
AAAATTGTGT ACAAGTCAGA GAGTGCGTGG CTGCCTGTCT ATATCAGAGC TGGAATTCCG 
AATCAGATTC CAAAGGCTGC ATTCATGGCC GTGTTTATTC TGGAAGTGGA TCAGTTCATC 
CTGACCTCCT TGACTACATC AGTTCTGGAC TGTGAAGAAG ATGAGACCCC TAAACCCTTG 
CTGGTGTTCA ACATTACTAA AGCCCCGCTC CAGGGCTATG TGACTCACCT GTTGGATCAC 
ACCAGACCAA TCTCCTCATT CACCTGGAAA GATCTCAGTG ACATGCAGAT CGCCTATCAG 
CCACCAAACA GCAGCCATTC TGAGAGGAGA CATGATGAGG TAGAATTGGA GGTATACGAC 
TTCTTCTTTG AAAGGAGTGC ACCTATGACA GTCCACATCT CCATCAGAAC AGCAGATACA 
AATGCCCCCC GTGTATCCTG GAATACAGGT CTGAGTCTCC TTGAGGGGCA GTCTCGAGCC 
ATCACTTGGG AACAGTTTCA GGTTGTCGAC AATGACGACA TTGGTGCTGT CCGGCTAGTC 
ACCGTTGGTG GCCTGCAGCA TGGATGGCTG ACTTTAAGAG GGGGGAAAGG GTTTCTCTTC 
ACCGTGGCTG ACCTCCAGGC TGGAGTTGTT CGCTATCATC ATGATGACAG CGACTCCACC 
AAAGACTTCG TGGTCTTCCG GATATTTGAT GGCCATCACA GCATCCGTCA CAAATTCCCC 
ATCAACGTCT TGCCCAAAGA TGATAGTCCC CCGTTCCTCA TAACCAATGT TGTGATTGAA 
CTGGAGGAGG GGCAGACCAT CCTGATCCAG GGATCCATGC TGCGAGCTTC AGATGTGGAC 
GCCAGTGATG ACTACATCTT CTTCAATATC ACAAAGCCTC CACAGGCTGG GGAGATCATG 
AAGAAGCCAG GGCCAGGACT GATAGGCTAT CCTGTCCATG GCTTCCTTCA GAGGGATTTG 
TTTAATGGAA TCATTTATTA TCGTCATTTT GGTGGAGAAA TCTTTGAAGA TTCTTTTCAA 
TTTGTCCTGT GGGACAGCCA TGAACCTCCA AATCTTTCAG TGCCACAGGT GGCAACAATC 
CATATAACTC CAGTGGATGA CCAGCTTCCA AAAGAGGCTC CTGGAGTTTC TCGGCATTTG 
GTTGTCAAGG AAACTGAGGT GGCCTATATA ACTAAGAAAC AGCTACATTT TATAGATTCA 
GAATCATATG ACAGGGAGCT GGTCTACACA ATAACTACTC CTCCATTTTT CTCCTTCAGC 
CACAGACACT TGGATGCTGG GAAATTATTT ATGGTGGACA GCATACCAAA AGTAGTTAAG 
AATCCTACGG CCCTGGAGCT GAGGTCATTC ACTCAGCATG CTGTGAACTA TATGAAAGTG 
GCCTACATGC CCCCCATGCA AGACATTGGT CCCCATTGCA GAGATGTCCA GTTCACATTT 
TCTGTCAGTA ACCAACATGG CGGTACTTTG CATGGGATCT GCTTTAACAT TACAATCCTC 
CCAGTGGACA ATCAAGTTCC TGAGGCGTTC ACCAACCCTC TGAAAGTGAC TGAGGGAGGT 
CAAAGCATCA TCAGCACAGA GCACATTCTA ATTTCTGATG CAGATACCAA GCTGGACAAT 
ATTGACCTCT CCCTGCGGGA ATTGCCTCTG CACGGAAGGG TGGAGCTGAA TGGATTTCCT 
CTAAATTCAG GGGGCACATT TTCTTGGGGC GATCTCCATA CCTTAAAAGT TAGGTATCAA 
CATGATGGAA CTGAAGTTCT TCAGGATGAC CTACTCTTGG AGGTCACCGA TGGCACAAAT 
TCAGCAGAAT TTGTACTACA TGTTGAGGTA TTCCCTGTCA ACGATGAGCC ACCAGTCTTA 
AAGGCTGACC TCATGCCTGT CATGAATTGC TCAGAGGGAG GAGAGGTGGT CATCACCTCT 
GAATACATTT TTGCTACTGA TGTGGACAGC GATAACTTGA AGTTGATGTT TGTGATTGCT 
CGCGAACCTC AGCATGGGGT GGTGAGGAGA GCTGGAGTCA CAGTGGATCA GTTCTCTCAG 
AGAGATGTTA TCTCAGAGGC CGTGACATAC AAACACACAG GTGGCGAGAT TGGCCTGATG 
CCTTGTTTTG ACACCATTAC ATTGGTGGTT TCGGATGGAG AGGCTGGCCC TTTTGTGAAT 
GGCTGTTGCT ACAATGGACC CAATCCATCT GTTCCACTTC ATGCGTCCTT TCCAGTGTAT 
GATCTCAACA TCACGGTATA CCCAGTAGAC AACCAGCCAC CTTCCATTGC AATAGGTCCC 
GTGTTTGTTG TAGATGAGGG CTGCTCAACA GCCCTTACTG TTAACCATTT GTCCGCCACT 
GACCCTGACA CTGCAGCAGA TGACTTGGAA TTTGTTTTGG TTTCTCCTCC TCAGTTTGGC 
TACCTCGAAA ATATACTCCC TTCTGTGGGT TTTGAAAAAA GCAATATTGG CATAAGTATA 
GATTCATTTC AGTGGAAAGA CATGAACGCT TTTCACATTA ACTATGTGCA GTCCAGGCAT 
CTGAGGATAG AACCAACTGC CGACCAGTTC ACGGTGTACG TCACAGATGG GAAGCATCAC 
TCCTTGGAGA TACCATTTTC TATTATAATC AACCCCACAA ATGATGAAGC TCCTGACTTT 
GTAGTGCAGA ATATTACTGT GTGTGAGGGT CAGATGAAAG AGCTGGACTC TTCCATCATC 
AGCGCTGTGG ACCTGGACAT TCCCCAGGAT GCCCTGCTGT TCAGCATCAC TCAAAAGCCA 
CGCCATGGCC TCCTCATCGA TAGGGGGTTT AGCAAAGACT TCTCTGAGAA TAAGCAGCCA 
GCCAACCCTC ACCAGAAACA TGCACCTGTT CACAGCTTTT CCATGGAACT CCTCAAGACT 
GGAATGAGGT TGACGTACAT GCATGATGAC TCAGAGAGCC TTGCTGATGA TTTTACAATC 
CAATTGTCAG ATGGGAAACA TAAGATACTT AAAACCATTT CAGTAGAGGT CATCCCAGTT 
AATGATGAAA AACCAATGCT GAGCAAGAAG GCTGAAATTG CAATGAATAT GGGTGAAACT 
CGTATTATTT CCAGTGCTAT TCTTTCAGCC ATAGATGAAG ACTCACCCAG GGAGAAGATT 
TACTATGTAT TTGAAAGGCT TCCCCAAAAT GGGCAACTTC AGCTTAAGAT AGGGAGGGAC 
TGGGTTCCTC TCTCCCCTGG CATGAAATGC ACTCAGGAGG AAGTGGATCT GAACTTGCTG 
AGATACACAC ACACCGGGGC AATGGATTCC CAGAATCAAG ATAGCTTCAC CTTCTACCTT 
TGGGATGGCA ACAACAGGTC CCCTGCTCTT GACTGTCAAA TCACCATCAA GGACATGGAA 
AAAGGTGATA TTGTCATCCT TACAAAACCA CTCGTGGTGT CTAAAGGTGA TAGAGGTTTC 
TTAACAACCA CCACGCTCCT GGCTGTGGAC GGAACAGACA AGCCTGAGGA ACTGCTCTAT 
GTCATCACCT CCCCTCCGCG ATATGGCCAG ATCGAATATG TTCACTATCC TGGAGTTCCC 
ATTACAAACT TCAGCCAAAT GGATGTAGTG GGGCAGACAG TTTGCTATGT ACACAAGAGC 
AAGGTGACTG TCTCCAGTGA CAGATTCAGA TTCATCATCA GCAATGGACT GCGGACCGAG 
CACGGGGTGT TTGAGATCAC ACTGGAGACT GTGGACAGAG CCCTGCCTGT GGTAACCAGG 
AACAAGGGGT TGAGACTGGC CCAAGGGGCC GTGGGCCTGC TTTCCCCTGA CCTCCTTCAG 
CTGACCGACC CTGATACACC TGCGGAGAAC CTCACCTTCC TCTTGGTTCA GCTCCCCCAG 
CATGGCCAGC TCTACCTGTG GGGGACAGGG CTACTTCAAC ACAATTTCAC CCAGCAGGAT 
GTGGACAGCA AGAATGTGGC CTATCGGCAC TCAGGAGGGG ACTCCCAGAC TGACTGCTTT 
ACTTTCATGG CCACAGATGG GACAAACCAA GGCTTTATTG TGAATGGGAG AGTGTGGGAA 
GAACCTGTTT TATTCACCAT TCAGGTAGAC CAATTGGACA AAACAGCTCC TCGTATCACA 
CTCTTGCATT CCCCTTCTCA AGTGGGGCTC CTGAAAAATG GCTGCTACGG GATTTACATC 
ACTTCCCGCG TGTTGAAGGC ATCAGACCCT GACACTGAGG ACGATCAGAT CATCTTTAAA 
ATTCTACAAG GCCCAAAACA TGGACATCTG GAGAACACAA CAACAGGTGA ATTTATCCAT 
GAGAAATTTA GCCAAAAGGA CTTAAACAGT AAGACTATTC TTTACATCAT AAACCCATCT 
TTGGAAGTAA ATTCAGATAC CGTGGAATTT CAAATCATGG ACCCCACAGG GAACTCGGCC 
ACTCCTCAAA TTTTGGAACT GAAGTGGTCT CATATTGAAT GGTCACAGAC CGAATATGAA 
GTCTGTGAGA ATGTGGGTTT GTTGCCCTTG GAAATTATCA GAAGGGGATA TTCCATGGAC 
TCGGCCTTTG TGGGTATAAA GGTCAACCAA GTGTCAGCTG CAGTTGGAAA AGATTTCACC 
GTGATTCCAT CTAAACTGAT TCAGTTTGAC CCAGGAATGT CAACTAAGAT GTGGAATATA 
GCAATTACCT ATGACGGATT AGAGGAAGAT GATGAGGTCT TTGAAGTAAT TCTGAACTCC 
CCTGTGAATG CAGTTCTTGG CACAAAGACA AAAGCTGCAG TGAAAATTTT GGACTCAAAA 
GGAGGACAAT GCCATCCTTC ATATTCCTCC AACCAAAGCA AGCACAGCAC ATGGGAGAAG 
GGCATTTGGC ATCTGCTGCC CCCAGGGTCT TCCTCATCCA CCACTTCTGG TTCCTTTCAT 
CTGGAAAGAA GACCTCTTCC ATCTTCCATG CAGCTAGCAG TCATCAGGGG AGACACCCTG 
CGGGGCTTTG ATTCTACAGA TCTTTCTCAA AGGAAGCTTA GGACCCGTGG GAATGGCAAA 
ACAGTTCGTC CATCCTCTGT TTATAGAAAT GGAACAGACA TCATCTATAA TTATCATGGG 
ATAGTTTCCT TGAAACTGGA GGATGACAGT TTCCCAACTC ACAAAAGGAA GGCCAAAGTA 
TCCATCATTA GTCAGCCACA AAAGACAATC AAAGTGGCAG AACTGCCTCA AGCAGATAAG 
GTGGAATCCA CAACTGACTC ACACTTCCCC AGACAGGACC AGTTGCCCTC ATTTCCAAAG 
AACTGCACTC TGGAATTAAA GGGACTCTTC CATTTTGAAG AAGGCATCCA GAAGCTGTAT 
CAGTGCAATG GGATCGCCTG GAAAGCCTGG AGTCCCCAAA CCAAGGATGT GGAAGACAAA 
TCCTGTCCAG CCGGGTGGCA CCAGCACTCA GGCTACTGTC ACATCTTGAT CACAGAGCAG 
AAAGGCACCT GGAATGCGGC TGCCCAAGCT TGCAGGGAAC AATACCTGGG CAACCTTGTA 
ACTGTATTCT CCAGGCAGCA CATGCGGTGG CTCTGGGACA TTGGTGGGAG AAAGTCCTTT 
TGGATAGGTT TGAACGACCA AGTGCATGCT GGCCACTGGG AGTGGATCGG TGGTGAACCT 
GTTGCCTTCA CCAATGGGAG AAGAGGGCCC TCTCAACGCT CCAAGCTTGG AAAGAGCTGT 
GTTTTGGTTC AAAGACAAGG GAAATGGCAA ACAAAAGACT GTAGGAGAGC CAAACCTCAT 
AATTATGTGT GTTCCAGAAA ACTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FREM1 ( XM_017014322.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016869811.1 Homo sapiens FRAS1 related extracellular matrix 1 (FREM1), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OHu98459
Clone ID Related Accession (Same CDS sequence)	XM_017014320.1 , XM_017014318.1 , XM_017014316.1 , XM_017014316.2 , XM_017014317.1 , XM_017014319.2 , XM_017014321.2 , XM_017014323.1 , XM_017014322.1 , XM_017014321.1 , XM_017014320.2 , XM_017014319.1
Accession Version	XM_017014322.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6567bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	FRAS1-related extracellular matrix protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACTCTC TGAGTTGGGG GGCTGCGAAT GCCGTGCTGC TGCTGCTCCT CCTGGCCTGG 
GCCAGCCCCA CCTTCATCAG CATCAACCGC GGGGTGAGGG TGATGAAGGG CCACTCTGCC 
TTCCTGTCAG GAGATGACCT GAAGTTTGCC ATCCCTAAAG AGAAAGATGC CTGCAAAGTG 
GAAGTTGTGA TGAATGAGCC AATAACCCAG AGGGTTGGGA AACTCACTCC ACAGGTCTTT 
GACTGCCATT TCCTTCCCAA CGAAGTCAAG TATGTTCACA ATGGTTGTCC AATTCTTGAT 
GAAGACACAG TGAAGCTCAG ACTTTACAGA TTTACTGAAA GAGATACCTT CATAGAAACT 
TTTATCCTGT GGGTCTATCT CCTGGAACCA GACTGTAACA TCATCCATAT GAGTAACAAT 
GTGCTGGAGG TGCCTGAATT CAATGGCTTG TCCCAAGCGA TTGATAAAAA TCTGCTCAGA 
TTCGATTATG ATAGGATGGC TAGCCTGGAA TGTACCGTCA GCCTGGACAC TGCGAGAACT 
CGGCTGCCAG CCCATGGCCA GATGGTTCTC GGGGAGCCTC GACCAGAAGA ACCTCGTGGA 
GATCAGCCAC ACAGTTTTTT CCCAGAGTCC CGTATGATGT CTTTTAATGC CTTTCTGGAA 
CTGAGAGCAA AACTGAAGTG TCCAGGTGGG AGCTGTACCC CAGGATTAAA GAAAATAGGA 
AGCCTCAAAG TGAGCTGTGA GGAGTTCCTG CTGATGGGCC TTCGTTATCA GCATCTGGAT 
CCCCCTTCAC CCAACATTGA TTATATCTCC ATCCAACTGG ACCTCACAGA TACCAGGAGC 
AAAATTGTGT ACAAGTCAGA GAGTGCGTGG CTGCCTGTCT ATATCAGAGC TGGAATTCCG 
AATCAGATTC CAAAGGCTGC ATTCATGGCC GTGTTTATTC TGGAAGTGGA TCAGTTCATC 
CTGACCTCCT TGACTACATC AGTTCTGGAC TGTGAAGAAG ATGAGACCCC TAAACCCTTG 
CTGGTGTTCA ACATTACTAA AGCCCCGCTC CAGGGCTATG TGACTCACCT GTTGGATCAC 
ACCAGACCAA TCTCCTCATT CACCTGGAAA GATCTCAGTG ACATGCAGAT CGCCTATCAG 
CCACCAAACA GCAGCCATTC TGAGAGGAGA CATGATGAGG TAGAATTGGA GGTATACGAC 
TTCTTCTTTG AAAGGAGTGC ACCTATGACA GTCCACATCT CCATCAGAAC AGCAGATACA 
AATGCCCCCC GTGTATCCTG GAATACAGGT CTGAGTCTCC TTGAGGGGCA GTCTCGAGCC 
ATCACTTGGG AACAGTTTCA GGTTGTCGAC AATGACGACA TTGGTGCTGT CCGGCTAGTC 
ACCGTTGGTG GCCTGCAGCA TGGATGGCTG ACTTTAAGAG GGGGGAAAGG GTTTCTCTTC 
ACCGTGGCTG ACCTCCAGGC TGGAGTTGTT CGCTATCATC ATGATGACAG CGACTCCACC 
AAAGACTTCG TGGTCTTCCG GATATTTGAT GGCCATCACA GCATCCGTCA CAAATTCCCC 
ATCAACGTCT TGCCCAAAGA TGATAGTCCC CCGTTCCTCA TAACCAATGT TGTGATTGAA 
CTGGAGGAGG GGCAGACCAT CCTGATCCAG GGATCCATGC TGCGAGCTTC AGATGTGGAC 
GCCAGTGATG ACTACATCTT CTTCAATATC ACAAAGCCTC CACAGGCTGG GGAGATCATG 
AAGAAGCCAG GGCCAGGACT GATAGGCTAT CCTGTCCATG GCTTCCTTCA GAGGGATTTG 
TTTAATGGAA TCATTTATTA TCGTCATTTT GGTGGAGAAA TCTTTGAAGA TTCTTTTCAA 
TTTGTCCTGT GGGACAGCCA TGAACCTCCA AATCTTTCAG TGCCACAGGT GGCAACAATC 
CATATAACTC CAGTGGATGA CCAGCTTCCA AAAGAGGCTC CTGGAGTTTC TCGGCATTTG 
GTTGTCAAGG AAACTGAGGT GGCCTATATA ACTAAGAAAC AGCTACATTT TATAGATTCA 
GAATCATATG ACAGGGAGCT GGTCTACACA ATAACTACTC CTCCATTTTT CTCCTTCAGC 
CACAGACACT TGGATGCTGG GAAATTATTT ATGGTGGACA GCATACCAAA AGTAGTTAAG 
AATCCTACGG CCCTGGAGCT GAGGTCATTC ACTCAGCATG CTGTGAACTA TATGAAAGTG 
GCCTACATGC CCCCCATGCA AGACATTGGT CCCCATTGCA GAGATGTCCA GTTCACATTT 
TCTGTCAGTA ACCAACATGG CGGTACTTTG CATGGGATCT GCTTTAACAT TACAATCCTC 
CCAGTGGACA ATCAAGTTCC TGAGGCGTTC ACCAACCCTC TGAAAGTGAC TGAGGGAGGT 
CAAAGCATCA TCAGCACAGA GCACATTCTA ATTTCTGATG CAGATACCAA GCTGGACAAT 
ATTGACCTCT CCCTGCGGGA ATTGCCTCTG CACGGAAGGG TGGAGCTGAA TGGATTTCCT 
CTAAATTCAG GGGGCACATT TTCTTGGGGC GATCTCCATA CCTTAAAAGT TAGGTATCAA 
CATGATGGAA CTGAAGTTCT TCAGGATGAC CTACTCTTGG AGGTCACCGA TGGCACAAAT 
TCAGCAGAAT TTGTACTACA TGTTGAGGTA TTCCCTGTCA ACGATGAGCC ACCAGTCTTA 
AAGGCTGACC TCATGCCTGT CATGAATTGC TCAGAGGGAG GAGAGGTGGT CATCACCTCT 
GAATACATTT TTGCTACTGA TGTGGACAGC GATAACTTGA AGTTGATGTT TGTGATTGCT 
CGCGAACCTC AGCATGGGGT GGTGAGGAGA GCTGGAGTCA CAGTGGATCA GTTCTCTCAG 
AGAGATGTTA TCTCAGAGGC CGTGACATAC AAACACACAG GTGGCGAGAT TGGCCTGATG 
CCTTGTTTTG ACACCATTAC ATTGGTGGTT TCGGATGGAG AGGCTGGCCC TTTTGTGAAT 
GGCTGTTGCT ACAATGGACC CAATCCATCT GTTCCACTTC ATGCGTCCTT TCCAGTGTAT 
GATCTCAACA TCACGGTATA CCCAGTAGAC AACCAGCCAC CTTCCATTGC AATAGGTCCC 
GTGTTTGTTG TAGATGAGGG CTGCTCAACA GCCCTTACTG TTAACCATTT GTCCGCCACT 
GACCCTGACA CTGCAGCAGA TGACTTGGAA TTTGTTTTGG TTTCTCCTCC TCAGTTTGGC 
TACCTCGAAA ATATACTCCC TTCTGTGGGT TTTGAAAAAA GCAATATTGG CATAAGTATA 
GATTCATTTC AGTGGAAAGA CATGAACGCT TTTCACATTA ACTATGTGCA GTCCAGGCAT 
CTGAGGATAG AACCAACTGC CGACCAGTTC ACGGTGTACG TCACAGATGG GAAGCATCAC 
TCCTTGGAGA TACCATTTTC TATTATAATC AACCCCACAA ATGATGAAGC TCCTGACTTT 
GTAGTGCAGA ATATTACTGT GTGTGAGGGT CAGATGAAAG AGCTGGACTC TTCCATCATC 
AGCGCTGTGG ACCTGGACAT TCCCCAGGAT GCCCTGCTGT TCAGCATCAC TCAAAAGCCA 
CGCCATGGCC TCCTCATCGA TAGGGGGTTT AGCAAAGACT TCTCTGAGAA TAAGCAGCCA 
GCCAACCCTC ACCAGAAACA TGCACCTGTT CACAGCTTTT CCATGGAACT CCTCAAGACT 
GGAATGAGGT TGACGTACAT GCATGATGAC TCAGAGAGCC TTGCTGATGA TTTTACAATC 
CAATTGTCAG ATGGGAAACA TAAGATACTT AAAACCATTT CAGTAGAGGT CATCCCAGTT 
AATGATGAAA AACCAATGCT GAGCAAGAAG GCTGAAATTG CAATGAATAT GGGTGAAACT 
CGTATTATTT CCAGTGCTAT TCTTTCAGCC ATAGATGAAG ACTCACCCAG GGAGAAGATT 
TACTATGTAT TTGAAAGGCT TCCCCAAAAT GGGCAACTTC AGCTTAAGAT AGGGAGGGAC 
TGGGTTCCTC TCTCCCCTGG CATGAAATGC ACTCAGGAGG AAGTGGATCT GAACTTGCTG 
AGATACACAC ACACCGGGGC AATGGATTCC CAGAATCAAG ATAGCTTCAC CTTCTACCTT 
TGGGATGGCA ACAACAGGTC CCCTGCTCTT GACTGTCAAA TCACCATCAA GGACATGGAA 
AAAGGTGATA TTGTCATCCT TACAAAACCA CTCGTGGTGT CTAAAGGTGA TAGAGGTTTC 
TTAACAACCA CCACGCTCCT GGCTGTGGAC GGAACAGACA AGCCTGAGGA ACTGCTCTAT 
GTCATCACCT CCCCTCCGCG ATATGGCCAG ATCGAATATG TTCACTATCC TGGAGTTCCC 
ATTACAAACT TCAGCCAAAT GGATGTAGTG GGGCAGACAG TTTGCTATGT ACACAAGAGC 
AAGGTGACTG TCTCCAGTGA CAGATTCAGA TTCATCATCA GCAATGGACT GCGGACCGAG 
CACGGGGTGT TTGAGATCAC ACTGGAGACT GTGGACAGAG CCCTGCCTGT GGTAACCAGG 
AACAAGGGGT TGAGACTGGC CCAAGGGGCC GTGGGCCTGC TTTCCCCTGA CCTCCTTCAG 
CTGACCGACC CTGATACACC TGCGGAGAAC CTCACCTTCC TCTTGGTTCA GCTCCCCCAG 
CATGGCCAGC TCTACCTGTG GGGGACAGGG CTACTTCAAC ACAATTTCAC CCAGCAGGAT 
GTGGACAGCA AGAATGTGGC CTATCGGCAC TCAGGAGGGG ACTCCCAGAC TGACTGCTTT 
ACTTTCATGG CCACAGATGG GACAAACCAA GGCTTTATTG TGAATGGGAG AGTGTGGGAA 
GAACCTGTTT TATTCACCAT TCAGGTAGAC CAATTGGACA AAACAGCTCC TCGTATCACA 
CTCTTGCATT CCCCTTCTCA AGTGGGGCTC CTGAAAAATG GCTGCTACGG GATTTACATC 
ACTTCCCGCG TGTTGAAGGC ATCAGACCCT GACACTGAGG ACGATCAGAT CATCTTTAAA 
ATTCTACAAG GCCCAAAACA TGGACATCTG GAGAACACAA CAACAGGTGA ATTTATCCAT 
GAGAAATTTA GCCAAAAGGA CTTAAACAGT AAGACTATTC TTTACATCAT AAACCCATCT 
TTGGAAGTAA ATTCAGATAC CGTGGAATTT CAAATCATGG ACCCCACAGG GAACTCGGCC 
ACTCCTCAAA TTTTGGAACT GAAGTGGTCT CATATTGAAT GGTCACAGAC CGAATATGAA 
GTCTGTGAGA ATGTGGGTTT GTTGCCCTTG GAAATTATCA GAAGGGGATA TTCCATGGAC 
TCGGCCTTTG TGGGTATAAA GGTCAACCAA GTGTCAGCTG CAGTTGGAAA AGATTTCACC 
GTGATTCCAT CTAAACTGAT TCAGTTTGAC CCAGGAATGT CAACTAAGAT GTGGAATATA 
GCAATTACCT ATGACGGATT AGAGGAAGAT GATGAGGTCT TTGAAGTAAT TCTGAACTCC 
CCTGTGAATG CAGTTCTTGG CACAAAGACA AAAGCTGCAG TGAAAATTTT GGACTCAAAA 
GGAGGACAAT GCCATCCTTC ATATTCCTCC AACCAAAGCA AGCACAGCAC ATGGGAGAAG 
GGCATTTGGC ATCTGCTGCC CCCAGGGTCT TCCTCATCCA CCACTTCTGG TTCCTTTCAT 
CTGGAAAGAA GACCTCTTCC ATCTTCCATG CAGCTAGCAG TCATCAGGGG AGACACCCTG 
CGGGGCTTTG ATTCTACAGA TCTTTCTCAA AGGAAGCTTA GGACCCGTGG GAATGGCAAA 
ACAGTTCGTC CATCCTCTGT TTATAGAAAT GGAACAGACA TCATCTATAA TTATCATGGG 
ATAGTTTCCT TGAAACTGGA GGATGACAGT TTCCCAACTC ACAAAAGGAA GGCCAAAGTA 
TCCATCATTA GTCAGCCACA AAAGACAATC AAAGTGGCAG AACTGCCTCA AGCAGATAAG 
GTGGAATCCA CAACTGACTC ACACTTCCCC AGACAGGACC AGTTGCCCTC ATTTCCAAAG 
AACTGCACTC TGGAATTAAA GGGACTCTTC CATTTTGAAG AAGGCATCCA GAAGCTGTAT 
CAGTGCAATG GGATCGCCTG GAAAGCCTGG AGTCCCCAAA CCAAGGATGT GGAAGACAAA 
TCCTGTCCAG CCGGGTGGCA CCAGCACTCA GGCTACTGTC ACATCTTGAT CACAGAGCAG 
AAAGGCACCT GGAATGCGGC TGCCCAAGCT TGCAGGGAAC AATACCTGGG CAACCTTGTA 
ACTGTATTCT CCAGGCAGCA CATGCGGTGG CTCTGGGACA TTGGTGGGAG AAAGTCCTTT 
TGGATAGGTT TGAACGACCA AGTGCATGCT GGCCACTGGG AGTGGATCGG TGGTGAACCT 
GTTGCCTTCA CCAATGGGAG AAGAGGGCCC TCTCAACGCT CCAAGCTTGG AAAGAGCTGT 
GTTTTGGTTC AAAGACAAGG GAAATGGCAA ACAAAAGACT GTAGGAGAGC CAAACCTCAT 
AATTATGTGT GTTCCAGAAA ACTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016869811.1
CDS	651..7217
Translation	MNSLSWGAANAVLLLLLLAWASPTFISINRGVRVMKGHSAFLSGDDLKFAIPKEKDACKVEVVMNEPITQRVGKLTPQVFDCHFLPNEVKYVHNGCPILDEDTVKLRLYRFTERDTFIETFILWVYLLEPDCNIIHMSNNVLEVPEFNGLSQAIDKNLLRFDYDRMASLECTVSLDTARTRLPAHGQMVLGEPRPEEPRGDQPHSFFPESRMMSFNAFLELRAKLKCPGGSCTPGLKKIGSLKVSCEEFLLMGLRYQHLDPPSPNIDYISIQLDLTDTRSKIVYKSESAWLPVYIRAGIPNQIPKAAFMAVFILEVDQFILTSLTTSVLDCEEDETPKPLLVFNITKAPLQGYVTHLLDHTRPISSFTWKDLSDMQIAYQPPNSSHSERRHDEVELEVYDFFFERSAPMTVHISIRTADTNAPRVSWNTGLSLLEGQSRAITWEQFQVVDNDDIGAVRLVTVGGLQHGWLTLRGGKGFLFTVADLQAGVVRYHHDDSDSTKDFVVFRIFDGHHSIRHKFPINVLPKDDSPPFLITNVVIELEEGQTILIQGSMLRASDVDASDDYIFFNITKPPQAGEIMKKPGPGLIGYPVHGFLQRDLFNGIIYYRHFGGEIFEDSFQFVLWDSHEPPNLSVPQVATIHITPVDDQLPKEAPGVSRHLVVKETEVAYITKKQLHFIDSESYDRELVYTITTPPFFSFSHRHLDAGKLFMVDSIPKVVKNPTALELRSFTQHAVNYMKVAYMPPMQDIGPHCRDVQFTFSVSNQHGGTLHGICFNITILPVDNQVPEAFTNPLKVTEGGQSIISTEHILISDADTKLDNIDLSLRELPLHGRVELNGFPLNSGGTFSWGDLHTLKVRYQHDGTEVLQDDLLLEVTDGTNSAEFVLHVEVFPVNDEPPVLKADLMPVMNCSEGGEVVITSEYIFATDVDSDNLKLMFVIAREPQHGVVRRAGVTVDQFSQRDVISEAVTYKHTGGEIGLMPCFDTITLVVSDGEAGPFVNGCCYNGPNPSVPLHASFPVYDLNITVYPVDNQPPSIAIGPVFVVDEGCSTALTVNHLSATDPDTAADDLEFVLVSPPQFGYLENILPSVGFEKSNIGISIDSFQWKDMNAFHINYVQSRHLRIEPTADQFTVYVTDGKHHSLEIPFSIIINPTNDEAPDFVVQNITVCEGQMKELDSSIISAVDLDIPQDALLFSITQKPRHGLLIDRGFSKDFSENKQPANPHQKHAPVHSFSMELLKTGMRLTYMHDDSESLADDFTIQLSDGKHKILKTISVEVIPVNDEKPMLSKKAEIAMNMGETRIISSAILSAIDEDSPREKIYYVFERLPQNGQLQLKIGRDWVPLSPGMKCTQEEVDLNLLRYTHTGAMDSQNQDSFTFYLWDGNNRSPALDCQITIKDMEKGDIVILTKPLVVSKGDRGFLTTTTLLAVDGTDKPEELLYVITSPPRYGQIEYVHYPGVPITNFSQMDVVGQTVCYVHKSKVTVSSDRFRFIISNGLRTEHGVFEITLETVDRALPVVTRNKGLRLAQGAVGLLSPDLLQLTDPDTPAENLTFLLVQLPQHGQLYLWGTGLLQHNFTQQDVDSKNVAYRHSGGDSQTDCFTFMATDGTNQGFIVNGRVWEEPVLFTIQVDQLDKTAPRITLLHSPSQVGLLKNGCYGIYITSRVLKASDPDTEDDQIIFKILQGPKHGHLENTTTGEFIHEKFSQKDLNSKTILYIINPSLEVNSDTVEFQIMDPTGNSATPQILELKWSHIEWSQTEYEVCENVGLLPLEIIRRGYSMDSAFVGIKVNQVSAAVGKDFTVIPSKLIQFDPGMSTKMWNIAITYDGLEEDDEVFEVILNSPVNAVLGTKTKAAVKILDSKGGQCHPSYSSNQSKHSTWEKGIWHLLPPGSSSSTTSGSFHLERRPLPSSMQLAVIRGDTLRGFDSTDLSQRKLRTRGNGKTVRPSSVYRNGTDIIYNYHGIVSLKLEDDSFPTHKRKAKVSIISQPQKTIKVAELPQADKVESTTDSHFPRQDQLPSFPKNCTLELKGLFHFEEGIQKLYQCNGIAWKAWSPQTKDVEDKSCPAGWHQHSGYCHILITEQKGTWNAAAQACREQYLGNLVTVFSRQHMRWLWDIGGRKSFWIGLNDQVHAGHWEWIGGEPVAFTNGRRGPSQRSKLGKSCVLVQRQGKWQTKDCRRAKPHNYVCSRKL

Target ORF information:

RefSeq Version	XM_017014322.1
Organism	Homo sapiens(human)
Definition	Homo sapiens FRAS1 related extracellular matrix 1 (FREM1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017014322.1

ORF Insert Sequence:

ATGAACTCTC TGAGTTGGGG GGCTGCGAAT GCCGTGCTGC TGCTGCTCCT CCTGGCCTGG 
GCCAGCCCCA CCTTCATCAG CATCAACCGC GGGGTGAGGG TGATGAAGGG CCACTCTGCC 
TTCCTGTCAG GAGATGACCT GAAGTTTGCC ATCCCTAAAG AGAAAGATGC CTGCAAAGTG 
GAAGTTGTGA TGAATGAGCC AATAACCCAG AGGGTTGGGA AACTCACTCC ACAGGTCTTT 
GACTGCCATT TCCTTCCCAA CGAAGTCAAG TATGTTCACA ATGGTTGTCC AATTCTTGAT 
GAAGACACAG TGAAGCTCAG ACTTTACAGA TTTACTGAAA GAGATACCTT CATAGAAACT 
TTTATCCTGT GGGTCTATCT CCTGGAACCA GACTGTAACA TCATCCATAT GAGTAACAAT 
GTGCTGGAGG TGCCTGAATT CAATGGCTTG TCCCAAGCGA TTGATAAAAA TCTGCTCAGA 
TTCGATTATG ATAGGATGGC TAGCCTGGAA TGTACCGTCA GCCTGGACAC TGCGAGAACT 
CGGCTGCCAG CCCATGGCCA GATGGTTCTC GGGGAGCCTC GACCAGAAGA ACCTCGTGGA 
GATCAGCCAC ACAGTTTTTT CCCAGAGTCC CGTATGATGT CTTTTAATGC CTTTCTGGAA 
CTGAGAGCAA AACTGAAGTG TCCAGGTGGG AGCTGTACCC CAGGATTAAA GAAAATAGGA 
AGCCTCAAAG TGAGCTGTGA GGAGTTCCTG CTGATGGGCC TTCGTTATCA GCATCTGGAT 
CCCCCTTCAC CCAACATTGA TTATATCTCC ATCCAACTGG ACCTCACAGA TACCAGGAGC 
AAAATTGTGT ACAAGTCAGA GAGTGCGTGG CTGCCTGTCT ATATCAGAGC TGGAATTCCG 
AATCAGATTC CAAAGGCTGC ATTCATGGCC GTGTTTATTC TGGAAGTGGA TCAGTTCATC 
CTGACCTCCT TGACTACATC AGTTCTGGAC TGTGAAGAAG ATGAGACCCC TAAACCCTTG 
CTGGTGTTCA ACATTACTAA AGCCCCGCTC CAGGGCTATG TGACTCACCT GTTGGATCAC 
ACCAGACCAA TCTCCTCATT CACCTGGAAA GATCTCAGTG ACATGCAGAT CGCCTATCAG 
CCACCAAACA GCAGCCATTC TGAGAGGAGA CATGATGAGG TAGAATTGGA GGTATACGAC 
TTCTTCTTTG AAAGGAGTGC ACCTATGACA GTCCACATCT CCATCAGAAC AGCAGATACA 
AATGCCCCCC GTGTATCCTG GAATACAGGT CTGAGTCTCC TTGAGGGGCA GTCTCGAGCC 
ATCACTTGGG AACAGTTTCA GGTTGTCGAC AATGACGACA TTGGTGCTGT CCGGCTAGTC 
ACCGTTGGTG GCCTGCAGCA TGGATGGCTG ACTTTAAGAG GGGGGAAAGG GTTTCTCTTC 
ACCGTGGCTG ACCTCCAGGC TGGAGTTGTT CGCTATCATC ATGATGACAG CGACTCCACC 
AAAGACTTCG TGGTCTTCCG GATATTTGAT GGCCATCACA GCATCCGTCA CAAATTCCCC 
ATCAACGTCT TGCCCAAAGA TGATAGTCCC CCGTTCCTCA TAACCAATGT TGTGATTGAA 
CTGGAGGAGG GGCAGACCAT CCTGATCCAG GGATCCATGC TGCGAGCTTC AGATGTGGAC 
GCCAGTGATG ACTACATCTT CTTCAATATC ACAAAGCCTC CACAGGCTGG GGAGATCATG 
AAGAAGCCAG GGCCAGGACT GATAGGCTAT CCTGTCCATG GCTTCCTTCA GAGGGATTTG 
TTTAATGGAA TCATTTATTA TCGTCATTTT GGTGGAGAAA TCTTTGAAGA TTCTTTTCAA 
TTTGTCCTGT GGGACAGCCA TGAACCTCCA AATCTTTCAG TGCCACAGGT GGCAACAATC 
CATATAACTC CAGTGGATGA CCAGCTTCCA AAAGAGGCTC CTGGAGTTTC TCGGCATTTG 
GTTGTCAAGG AAACTGAGGT GGCCTATATA ACTAAGAAAC AGCTACATTT TATAGATTCA 
GAATCATATG ACAGGGAGCT GGTCTACACA ATAACTACTC CTCCATTTTT CTCCTTCAGC 
CACAGACACT TGGATGCTGG GAAATTATTT ATGGTGGACA GCATACCAAA AGTAGTTAAG 
AATCCTACGG CCCTGGAGCT GAGGTCATTC ACTCAGCATG CTGTGAACTA TATGAAAGTG 
GCCTACATGC CCCCCATGCA AGACATTGGT CCCCATTGCA GAGATGTCCA GTTCACATTT 
TCTGTCAGTA ACCAACATGG CGGTACTTTG CATGGGATCT GCTTTAACAT TACAATCCTC 
CCAGTGGACA ATCAAGTTCC TGAGGCGTTC ACCAACCCTC TGAAAGTGAC TGAGGGAGGT 
CAAAGCATCA TCAGCACAGA GCACATTCTA ATTTCTGATG CAGATACCAA GCTGGACAAT 
ATTGACCTCT CCCTGCGGGA ATTGCCTCTG CACGGAAGGG TGGAGCTGAA TGGATTTCCT 
CTAAATTCAG GGGGCACATT TTCTTGGGGC GATCTCCATA CCTTAAAAGT TAGGTATCAA 
CATGATGGAA CTGAAGTTCT TCAGGATGAC CTACTCTTGG AGGTCACCGA TGGCACAAAT 
TCAGCAGAAT TTGTACTACA TGTTGAGGTA TTCCCTGTCA ACGATGAGCC ACCAGTCTTA 
AAGGCTGACC TCATGCCTGT CATGAATTGC TCAGAGGGAG GAGAGGTGGT CATCACCTCT 
GAATACATTT TTGCTACTGA TGTGGACAGC GATAACTTGA AGTTGATGTT TGTGATTGCT 
CGCGAACCTC AGCATGGGGT GGTGAGGAGA GCTGGAGTCA CAGTGGATCA GTTCTCTCAG 
AGAGATGTTA TCTCAGAGGC CGTGACATAC AAACACACAG GTGGCGAGAT TGGCCTGATG 
CCTTGTTTTG ACACCATTAC ATTGGTGGTT TCGGATGGAG AGGCTGGCCC TTTTGTGAAT 
GGCTGTTGCT ACAATGGACC CAATCCATCT GTTCCACTTC ATGCGTCCTT TCCAGTGTAT 
GATCTCAACA TCACGGTATA CCCAGTAGAC AACCAGCCAC CTTCCATTGC AATAGGTCCC 
GTGTTTGTTG TAGATGAGGG CTGCTCAACA GCCCTTACTG TTAACCATTT GTCCGCCACT 
GACCCTGACA CTGCAGCAGA TGACTTGGAA TTTGTTTTGG TTTCTCCTCC TCAGTTTGGC 
TACCTCGAAA ATATACTCCC TTCTGTGGGT TTTGAAAAAA GCAATATTGG CATAAGTATA 
GATTCATTTC AGTGGAAAGA CATGAACGCT TTTCACATTA ACTATGTGCA GTCCAGGCAT 
CTGAGGATAG AACCAACTGC CGACCAGTTC ACGGTGTACG TCACAGATGG GAAGCATCAC 
TCCTTGGAGA TACCATTTTC TATTATAATC AACCCCACAA ATGATGAAGC TCCTGACTTT 
GTAGTGCAGA ATATTACTGT GTGTGAGGGT CAGATGAAAG AGCTGGACTC TTCCATCATC 
AGCGCTGTGG ACCTGGACAT TCCCCAGGAT GCCCTGCTGT TCAGCATCAC TCAAAAGCCA 
CGCCATGGCC TCCTCATCGA TAGGGGGTTT AGCAAAGACT TCTCTGAGAA TAAGCAGCCA 
GCCAACCCTC ACCAGAAACA TGCACCTGTT CACAGCTTTT CCATGGAACT CCTCAAGACT 
GGAATGAGGT TGACGTACAT GCATGATGAC TCAGAGAGCC TTGCTGATGA TTTTACAATC 
CAATTGTCAG ATGGGAAACA TAAGATACTT AAAACCATTT CAGTAGAGGT CATCCCAGTT 
AATGATGAAA AACCAATGCT GAGCAAGAAG GCTGAAATTG CAATGAATAT GGGTGAAACT 
CGTATTATTT CCAGTGCTAT TCTTTCAGCC ATAGATGAAG ACTCACCCAG GGAGAAGATT 
TACTATGTAT TTGAAAGGCT TCCCCAAAAT GGGCAACTTC AGCTTAAGAT AGGGAGGGAC 
TGGGTTCCTC TCTCCCCTGG CATGAAATGC ACTCAGGAGG AAGTGGATCT GAACTTGCTG 
AGATACACAC ACACCGGGGC AATGGATTCC CAGAATCAAG ATAGCTTCAC CTTCTACCTT 
TGGGATGGCA ACAACAGGTC CCCTGCTCTT GACTGTCAAA TCACCATCAA GGACATGGAA 
AAAGGTGATA TTGTCATCCT TACAAAACCA CTCGTGGTGT CTAAAGGTGA TAGAGGTTTC 
TTAACAACCA CCACGCTCCT GGCTGTGGAC GGAACAGACA AGCCTGAGGA ACTGCTCTAT 
GTCATCACCT CCCCTCCGCG ATATGGCCAG ATCGAATATG TTCACTATCC TGGAGTTCCC 
ATTACAAACT TCAGCCAAAT GGATGTAGTG GGGCAGACAG TTTGCTATGT ACACAAGAGC 
AAGGTGACTG TCTCCAGTGA CAGATTCAGA TTCATCATCA GCAATGGACT GCGGACCGAG 
CACGGGGTGT TTGAGATCAC ACTGGAGACT GTGGACAGAG CCCTGCCTGT GGTAACCAGG 
AACAAGGGGT TGAGACTGGC CCAAGGGGCC GTGGGCCTGC TTTCCCCTGA CCTCCTTCAG 
CTGACCGACC CTGATACACC TGCGGAGAAC CTCACCTTCC TCTTGGTTCA GCTCCCCCAG 
CATGGCCAGC TCTACCTGTG GGGGACAGGG CTACTTCAAC ACAATTTCAC CCAGCAGGAT 
GTGGACAGCA AGAATGTGGC CTATCGGCAC TCAGGAGGGG ACTCCCAGAC TGACTGCTTT 
ACTTTCATGG CCACAGATGG GACAAACCAA GGCTTTATTG TGAATGGGAG AGTGTGGGAA 
GAACCTGTTT TATTCACCAT TCAGGTAGAC CAATTGGACA AAACAGCTCC TCGTATCACA 
CTCTTGCATT CCCCTTCTCA AGTGGGGCTC CTGAAAAATG GCTGCTACGG GATTTACATC 
ACTTCCCGCG TGTTGAAGGC ATCAGACCCT GACACTGAGG ACGATCAGAT CATCTTTAAA 
ATTCTACAAG GCCCAAAACA TGGACATCTG GAGAACACAA CAACAGGTGA ATTTATCCAT 
GAGAAATTTA GCCAAAAGGA CTTAAACAGT AAGACTATTC TTTACATCAT AAACCCATCT 
TTGGAAGTAA ATTCAGATAC CGTGGAATTT CAAATCATGG ACCCCACAGG GAACTCGGCC 
ACTCCTCAAA TTTTGGAACT GAAGTGGTCT CATATTGAAT GGTCACAGAC CGAATATGAA 
GTCTGTGAGA ATGTGGGTTT GTTGCCCTTG GAAATTATCA GAAGGGGATA TTCCATGGAC 
TCGGCCTTTG TGGGTATAAA GGTCAACCAA GTGTCAGCTG CAGTTGGAAA AGATTTCACC 
GTGATTCCAT CTAAACTGAT TCAGTTTGAC CCAGGAATGT CAACTAAGAT GTGGAATATA 
GCAATTACCT ATGACGGATT AGAGGAAGAT GATGAGGTCT TTGAAGTAAT TCTGAACTCC 
CCTGTGAATG CAGTTCTTGG CACAAAGACA AAAGCTGCAG TGAAAATTTT GGACTCAAAA 
GGAGGACAAT GCCATCCTTC ATATTCCTCC AACCAAAGCA AGCACAGCAC ATGGGAGAAG 
GGCATTTGGC ATCTGCTGCC CCCAGGGTCT TCCTCATCCA CCACTTCTGG TTCCTTTCAT 
CTGGAAAGAA GACCTCTTCC ATCTTCCATG CAGCTAGCAG TCATCAGGGG AGACACCCTG 
CGGGGCTTTG ATTCTACAGA TCTTTCTCAA AGGAAGCTTA GGACCCGTGG GAATGGCAAA 
ACAGTTCGTC CATCCTCTGT TTATAGAAAT GGAACAGACA TCATCTATAA TTATCATGGG 
ATAGTTTCCT TGAAACTGGA GGATGACAGT TTCCCAACTC ACAAAAGGAA GGCCAAAGTA 
TCCATCATTA GTCAGCCACA AAAGACAATC AAAGTGGCAG AACTGCCTCA AGCAGATAAG 
GTGGAATCCA CAACTGACTC ACACTTCCCC AGACAGGACC AGTTGCCCTC ATTTCCAAAG 
AACTGCACTC TGGAATTAAA GGGACTCTTC CATTTTGAAG AAGGCATCCA GAAGCTGTAT 
CAGTGCAATG GGATCGCCTG GAAAGCCTGG AGTCCCCAAA CCAAGGATGT GGAAGACAAA 
TCCTGTCCAG CCGGGTGGCA CCAGCACTCA GGCTACTGTC ACATCTTGAT CACAGAGCAG 
AAAGGCACCT GGAATGCGGC TGCCCAAGCT TGCAGGGAAC AATACCTGGG CAACCTTGTA 
ACTGTATTCT CCAGGCAGCA CATGCGGTGG CTCTGGGACA TTGGTGGGAG AAAGTCCTTT 
TGGATAGGTT TGAACGACCA AGTGCATGCT GGCCACTGGG AGTGGATCGG TGGTGAACCT 
GTTGCCTTCA CCAATGGGAG AAGAGGGCCC TCTCAACGCT CCAAGCTTGG AAAGAGCTGT 
GTTTTGGTTC AAAGACAAGG GAAATGGCAA ACAAAAGACT GTAGGAGAGC CAAACCTCAT 
AATTATGTGT GTTCCAGAAA ACTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FREM1 ( XM_017014321.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016869810.1 Homo sapiens FRAS1 related extracellular matrix 1 (FREM1), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OHu98459
Clone ID Related Accession (Same CDS sequence)	XM_017014320.1 , XM_017014318.1 , XM_017014316.1 , XM_017014316.2 , XM_017014317.1 , XM_017014319.2 , XM_017014321.2 , XM_017014323.1 , XM_017014322.1 , XM_017014321.1 , XM_017014320.2 , XM_017014319.1
Accession Version	XM_017014321.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6567bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	FRAS1-related extracellular matrix protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACTCTC TGAGTTGGGG GGCTGCGAAT GCCGTGCTGC TGCTGCTCCT CCTGGCCTGG 
GCCAGCCCCA CCTTCATCAG CATCAACCGC GGGGTGAGGG TGATGAAGGG CCACTCTGCC 
TTCCTGTCAG GAGATGACCT GAAGTTTGCC ATCCCTAAAG AGAAAGATGC CTGCAAAGTG 
GAAGTTGTGA TGAATGAGCC AATAACCCAG AGGGTTGGGA AACTCACTCC ACAGGTCTTT 
GACTGCCATT TCCTTCCCAA CGAAGTCAAG TATGTTCACA ATGGTTGTCC AATTCTTGAT 
GAAGACACAG TGAAGCTCAG ACTTTACAGA TTTACTGAAA GAGATACCTT CATAGAAACT 
TTTATCCTGT GGGTCTATCT CCTGGAACCA GACTGTAACA TCATCCATAT GAGTAACAAT 
GTGCTGGAGG TGCCTGAATT CAATGGCTTG TCCCAAGCGA TTGATAAAAA TCTGCTCAGA 
TTCGATTATG ATAGGATGGC TAGCCTGGAA TGTACCGTCA GCCTGGACAC TGCGAGAACT 
CGGCTGCCAG CCCATGGCCA GATGGTTCTC GGGGAGCCTC GACCAGAAGA ACCTCGTGGA 
GATCAGCCAC ACAGTTTTTT CCCAGAGTCC CGTATGATGT CTTTTAATGC CTTTCTGGAA 
CTGAGAGCAA AACTGAAGTG TCCAGGTGGG AGCTGTACCC CAGGATTAAA GAAAATAGGA 
AGCCTCAAAG TGAGCTGTGA GGAGTTCCTG CTGATGGGCC TTCGTTATCA GCATCTGGAT 
CCCCCTTCAC CCAACATTGA TTATATCTCC ATCCAACTGG ACCTCACAGA TACCAGGAGC 
AAAATTGTGT ACAAGTCAGA GAGTGCGTGG CTGCCTGTCT ATATCAGAGC TGGAATTCCG 
AATCAGATTC CAAAGGCTGC ATTCATGGCC GTGTTTATTC TGGAAGTGGA TCAGTTCATC 
CTGACCTCCT TGACTACATC AGTTCTGGAC TGTGAAGAAG ATGAGACCCC TAAACCCTTG 
CTGGTGTTCA ACATTACTAA AGCCCCGCTC CAGGGCTATG TGACTCACCT GTTGGATCAC 
ACCAGACCAA TCTCCTCATT CACCTGGAAA GATCTCAGTG ACATGCAGAT CGCCTATCAG 
CCACCAAACA GCAGCCATTC TGAGAGGAGA CATGATGAGG TAGAATTGGA GGTATACGAC 
TTCTTCTTTG AAAGGAGTGC ACCTATGACA GTCCACATCT CCATCAGAAC AGCAGATACA 
AATGCCCCCC GTGTATCCTG GAATACAGGT CTGAGTCTCC TTGAGGGGCA GTCTCGAGCC 
ATCACTTGGG AACAGTTTCA GGTTGTCGAC AATGACGACA TTGGTGCTGT CCGGCTAGTC 
ACCGTTGGTG GCCTGCAGCA TGGATGGCTG ACTTTAAGAG GGGGGAAAGG GTTTCTCTTC 
ACCGTGGCTG ACCTCCAGGC TGGAGTTGTT CGCTATCATC ATGATGACAG CGACTCCACC 
AAAGACTTCG TGGTCTTCCG GATATTTGAT GGCCATCACA GCATCCGTCA CAAATTCCCC 
ATCAACGTCT TGCCCAAAGA TGATAGTCCC CCGTTCCTCA TAACCAATGT TGTGATTGAA 
CTGGAGGAGG GGCAGACCAT CCTGATCCAG GGATCCATGC TGCGAGCTTC AGATGTGGAC 
GCCAGTGATG ACTACATCTT CTTCAATATC ACAAAGCCTC CACAGGCTGG GGAGATCATG 
AAGAAGCCAG GGCCAGGACT GATAGGCTAT CCTGTCCATG GCTTCCTTCA GAGGGATTTG 
TTTAATGGAA TCATTTATTA TCGTCATTTT GGTGGAGAAA TCTTTGAAGA TTCTTTTCAA 
TTTGTCCTGT GGGACAGCCA TGAACCTCCA AATCTTTCAG TGCCACAGGT GGCAACAATC 
CATATAACTC CAGTGGATGA CCAGCTTCCA AAAGAGGCTC CTGGAGTTTC TCGGCATTTG 
GTTGTCAAGG AAACTGAGGT GGCCTATATA ACTAAGAAAC AGCTACATTT TATAGATTCA 
GAATCATATG ACAGGGAGCT GGTCTACACA ATAACTACTC CTCCATTTTT CTCCTTCAGC 
CACAGACACT TGGATGCTGG GAAATTATTT ATGGTGGACA GCATACCAAA AGTAGTTAAG 
AATCCTACGG CCCTGGAGCT GAGGTCATTC ACTCAGCATG CTGTGAACTA TATGAAAGTG 
GCCTACATGC CCCCCATGCA AGACATTGGT CCCCATTGCA GAGATGTCCA GTTCACATTT 
TCTGTCAGTA ACCAACATGG CGGTACTTTG CATGGGATCT GCTTTAACAT TACAATCCTC 
CCAGTGGACA ATCAAGTTCC TGAGGCGTTC ACCAACCCTC TGAAAGTGAC TGAGGGAGGT 
CAAAGCATCA TCAGCACAGA GCACATTCTA ATTTCTGATG CAGATACCAA GCTGGACAAT 
ATTGACCTCT CCCTGCGGGA ATTGCCTCTG CACGGAAGGG TGGAGCTGAA TGGATTTCCT 
CTAAATTCAG GGGGCACATT TTCTTGGGGC GATCTCCATA CCTTAAAAGT TAGGTATCAA 
CATGATGGAA CTGAAGTTCT TCAGGATGAC CTACTCTTGG AGGTCACCGA TGGCACAAAT 
TCAGCAGAAT TTGTACTACA TGTTGAGGTA TTCCCTGTCA ACGATGAGCC ACCAGTCTTA 
AAGGCTGACC TCATGCCTGT CATGAATTGC TCAGAGGGAG GAGAGGTGGT CATCACCTCT 
GAATACATTT TTGCTACTGA TGTGGACAGC GATAACTTGA AGTTGATGTT TGTGATTGCT 
CGCGAACCTC AGCATGGGGT GGTGAGGAGA GCTGGAGTCA CAGTGGATCA GTTCTCTCAG 
AGAGATGTTA TCTCAGAGGC CGTGACATAC AAACACACAG GTGGCGAGAT TGGCCTGATG 
CCTTGTTTTG ACACCATTAC ATTGGTGGTT TCGGATGGAG AGGCTGGCCC TTTTGTGAAT 
GGCTGTTGCT ACAATGGACC CAATCCATCT GTTCCACTTC ATGCGTCCTT TCCAGTGTAT 
GATCTCAACA TCACGGTATA CCCAGTAGAC AACCAGCCAC CTTCCATTGC AATAGGTCCC 
GTGTTTGTTG TAGATGAGGG CTGCTCAACA GCCCTTACTG TTAACCATTT GTCCGCCACT 
GACCCTGACA CTGCAGCAGA TGACTTGGAA TTTGTTTTGG TTTCTCCTCC TCAGTTTGGC 
TACCTCGAAA ATATACTCCC TTCTGTGGGT TTTGAAAAAA GCAATATTGG CATAAGTATA 
GATTCATTTC AGTGGAAAGA CATGAACGCT TTTCACATTA ACTATGTGCA GTCCAGGCAT 
CTGAGGATAG AACCAACTGC CGACCAGTTC ACGGTGTACG TCACAGATGG GAAGCATCAC 
TCCTTGGAGA TACCATTTTC TATTATAATC AACCCCACAA ATGATGAAGC TCCTGACTTT 
GTAGTGCAGA ATATTACTGT GTGTGAGGGT CAGATGAAAG AGCTGGACTC TTCCATCATC 
AGCGCTGTGG ACCTGGACAT TCCCCAGGAT GCCCTGCTGT TCAGCATCAC TCAAAAGCCA 
CGCCATGGCC TCCTCATCGA TAGGGGGTTT AGCAAAGACT TCTCTGAGAA TAAGCAGCCA 
GCCAACCCTC ACCAGAAACA TGCACCTGTT CACAGCTTTT CCATGGAACT CCTCAAGACT 
GGAATGAGGT TGACGTACAT GCATGATGAC TCAGAGAGCC TTGCTGATGA TTTTACAATC 
CAATTGTCAG ATGGGAAACA TAAGATACTT AAAACCATTT CAGTAGAGGT CATCCCAGTT 
AATGATGAAA AACCAATGCT GAGCAAGAAG GCTGAAATTG CAATGAATAT GGGTGAAACT 
CGTATTATTT CCAGTGCTAT TCTTTCAGCC ATAGATGAAG ACTCACCCAG GGAGAAGATT 
TACTATGTAT TTGAAAGGCT TCCCCAAAAT GGGCAACTTC AGCTTAAGAT AGGGAGGGAC 
TGGGTTCCTC TCTCCCCTGG CATGAAATGC ACTCAGGAGG AAGTGGATCT GAACTTGCTG 
AGATACACAC ACACCGGGGC AATGGATTCC CAGAATCAAG ATAGCTTCAC CTTCTACCTT 
TGGGATGGCA ACAACAGGTC CCCTGCTCTT GACTGTCAAA TCACCATCAA GGACATGGAA 
AAAGGTGATA TTGTCATCCT TACAAAACCA CTCGTGGTGT CTAAAGGTGA TAGAGGTTTC 
TTAACAACCA CCACGCTCCT GGCTGTGGAC GGAACAGACA AGCCTGAGGA ACTGCTCTAT 
GTCATCACCT CCCCTCCGCG ATATGGCCAG ATCGAATATG TTCACTATCC TGGAGTTCCC 
ATTACAAACT TCAGCCAAAT GGATGTAGTG GGGCAGACAG TTTGCTATGT ACACAAGAGC 
AAGGTGACTG TCTCCAGTGA CAGATTCAGA TTCATCATCA GCAATGGACT GCGGACCGAG 
CACGGGGTGT TTGAGATCAC ACTGGAGACT GTGGACAGAG CCCTGCCTGT GGTAACCAGG 
AACAAGGGGT TGAGACTGGC CCAAGGGGCC GTGGGCCTGC TTTCCCCTGA CCTCCTTCAG 
CTGACCGACC CTGATACACC TGCGGAGAAC CTCACCTTCC TCTTGGTTCA GCTCCCCCAG 
CATGGCCAGC TCTACCTGTG GGGGACAGGG CTACTTCAAC ACAATTTCAC CCAGCAGGAT 
GTGGACAGCA AGAATGTGGC CTATCGGCAC TCAGGAGGGG ACTCCCAGAC TGACTGCTTT 
ACTTTCATGG CCACAGATGG GACAAACCAA GGCTTTATTG TGAATGGGAG AGTGTGGGAA 
GAACCTGTTT TATTCACCAT TCAGGTAGAC CAATTGGACA AAACAGCTCC TCGTATCACA 
CTCTTGCATT CCCCTTCTCA AGTGGGGCTC CTGAAAAATG GCTGCTACGG GATTTACATC 
ACTTCCCGCG TGTTGAAGGC ATCAGACCCT GACACTGAGG ACGATCAGAT CATCTTTAAA 
ATTCTACAAG GCCCAAAACA TGGACATCTG GAGAACACAA CAACAGGTGA ATTTATCCAT 
GAGAAATTTA GCCAAAAGGA CTTAAACAGT AAGACTATTC TTTACATCAT AAACCCATCT 
TTGGAAGTAA ATTCAGATAC CGTGGAATTT CAAATCATGG ACCCCACAGG GAACTCGGCC 
ACTCCTCAAA TTTTGGAACT GAAGTGGTCT CATATTGAAT GGTCACAGAC CGAATATGAA 
GTCTGTGAGA ATGTGGGTTT GTTGCCCTTG GAAATTATCA GAAGGGGATA TTCCATGGAC 
TCGGCCTTTG TGGGTATAAA GGTCAACCAA GTGTCAGCTG CAGTTGGAAA AGATTTCACC 
GTGATTCCAT CTAAACTGAT TCAGTTTGAC CCAGGAATGT CAACTAAGAT GTGGAATATA 
GCAATTACCT ATGACGGATT AGAGGAAGAT GATGAGGTCT TTGAAGTAAT TCTGAACTCC 
CCTGTGAATG CAGTTCTTGG CACAAAGACA AAAGCTGCAG TGAAAATTTT GGACTCAAAA 
GGAGGACAAT GCCATCCTTC ATATTCCTCC AACCAAAGCA AGCACAGCAC ATGGGAGAAG 
GGCATTTGGC ATCTGCTGCC CCCAGGGTCT TCCTCATCCA CCACTTCTGG TTCCTTTCAT 
CTGGAAAGAA GACCTCTTCC ATCTTCCATG CAGCTAGCAG TCATCAGGGG AGACACCCTG 
CGGGGCTTTG ATTCTACAGA TCTTTCTCAA AGGAAGCTTA GGACCCGTGG GAATGGCAAA 
ACAGTTCGTC CATCCTCTGT TTATAGAAAT GGAACAGACA TCATCTATAA TTATCATGGG 
ATAGTTTCCT TGAAACTGGA GGATGACAGT TTCCCAACTC ACAAAAGGAA GGCCAAAGTA 
TCCATCATTA GTCAGCCACA AAAGACAATC AAAGTGGCAG AACTGCCTCA AGCAGATAAG 
GTGGAATCCA CAACTGACTC ACACTTCCCC AGACAGGACC AGTTGCCCTC ATTTCCAAAG 
AACTGCACTC TGGAATTAAA GGGACTCTTC CATTTTGAAG AAGGCATCCA GAAGCTGTAT 
CAGTGCAATG GGATCGCCTG GAAAGCCTGG AGTCCCCAAA CCAAGGATGT GGAAGACAAA 
TCCTGTCCAG CCGGGTGGCA CCAGCACTCA GGCTACTGTC ACATCTTGAT CACAGAGCAG 
AAAGGCACCT GGAATGCGGC TGCCCAAGCT TGCAGGGAAC AATACCTGGG CAACCTTGTA 
ACTGTATTCT CCAGGCAGCA CATGCGGTGG CTCTGGGACA TTGGTGGGAG AAAGTCCTTT 
TGGATAGGTT TGAACGACCA AGTGCATGCT GGCCACTGGG AGTGGATCGG TGGTGAACCT 
GTTGCCTTCA CCAATGGGAG AAGAGGGCCC TCTCAACGCT CCAAGCTTGG AAAGAGCTGT 
GTTTTGGTTC AAAGACAAGG GAAATGGCAA ACAAAAGACT GTAGGAGAGC CAAACCTCAT 
AATTATGTGT GTTCCAGAAA ACTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016869810.1
CDS	857..7423
Translation	MNSLSWGAANAVLLLLLLAWASPTFISINRGVRVMKGHSAFLSGDDLKFAIPKEKDACKVEVVMNEPITQRVGKLTPQVFDCHFLPNEVKYVHNGCPILDEDTVKLRLYRFTERDTFIETFILWVYLLEPDCNIIHMSNNVLEVPEFNGLSQAIDKNLLRFDYDRMASLECTVSLDTARTRLPAHGQMVLGEPRPEEPRGDQPHSFFPESRMMSFNAFLELRAKLKCPGGSCTPGLKKIGSLKVSCEEFLLMGLRYQHLDPPSPNIDYISIQLDLTDTRSKIVYKSESAWLPVYIRAGIPNQIPKAAFMAVFILEVDQFILTSLTTSVLDCEEDETPKPLLVFNITKAPLQGYVTHLLDHTRPISSFTWKDLSDMQIAYQPPNSSHSERRHDEVELEVYDFFFERSAPMTVHISIRTADTNAPRVSWNTGLSLLEGQSRAITWEQFQVVDNDDIGAVRLVTVGGLQHGWLTLRGGKGFLFTVADLQAGVVRYHHDDSDSTKDFVVFRIFDGHHSIRHKFPINVLPKDDSPPFLITNVVIELEEGQTILIQGSMLRASDVDASDDYIFFNITKPPQAGEIMKKPGPGLIGYPVHGFLQRDLFNGIIYYRHFGGEIFEDSFQFVLWDSHEPPNLSVPQVATIHITPVDDQLPKEAPGVSRHLVVKETEVAYITKKQLHFIDSESYDRELVYTITTPPFFSFSHRHLDAGKLFMVDSIPKVVKNPTALELRSFTQHAVNYMKVAYMPPMQDIGPHCRDVQFTFSVSNQHGGTLHGICFNITILPVDNQVPEAFTNPLKVTEGGQSIISTEHILISDADTKLDNIDLSLRELPLHGRVELNGFPLNSGGTFSWGDLHTLKVRYQHDGTEVLQDDLLLEVTDGTNSAEFVLHVEVFPVNDEPPVLKADLMPVMNCSEGGEVVITSEYIFATDVDSDNLKLMFVIAREPQHGVVRRAGVTVDQFSQRDVISEAVTYKHTGGEIGLMPCFDTITLVVSDGEAGPFVNGCCYNGPNPSVPLHASFPVYDLNITVYPVDNQPPSIAIGPVFVVDEGCSTALTVNHLSATDPDTAADDLEFVLVSPPQFGYLENILPSVGFEKSNIGISIDSFQWKDMNAFHINYVQSRHLRIEPTADQFTVYVTDGKHHSLEIPFSIIINPTNDEAPDFVVQNITVCEGQMKELDSSIISAVDLDIPQDALLFSITQKPRHGLLIDRGFSKDFSENKQPANPHQKHAPVHSFSMELLKTGMRLTYMHDDSESLADDFTIQLSDGKHKILKTISVEVIPVNDEKPMLSKKAEIAMNMGETRIISSAILSAIDEDSPREKIYYVFERLPQNGQLQLKIGRDWVPLSPGMKCTQEEVDLNLLRYTHTGAMDSQNQDSFTFYLWDGNNRSPALDCQITIKDMEKGDIVILTKPLVVSKGDRGFLTTTTLLAVDGTDKPEELLYVITSPPRYGQIEYVHYPGVPITNFSQMDVVGQTVCYVHKSKVTVSSDRFRFIISNGLRTEHGVFEITLETVDRALPVVTRNKGLRLAQGAVGLLSPDLLQLTDPDTPAENLTFLLVQLPQHGQLYLWGTGLLQHNFTQQDVDSKNVAYRHSGGDSQTDCFTFMATDGTNQGFIVNGRVWEEPVLFTIQVDQLDKTAPRITLLHSPSQVGLLKNGCYGIYITSRVLKASDPDTEDDQIIFKILQGPKHGHLENTTTGEFIHEKFSQKDLNSKTILYIINPSLEVNSDTVEFQIMDPTGNSATPQILELKWSHIEWSQTEYEVCENVGLLPLEIIRRGYSMDSAFVGIKVNQVSAAVGKDFTVIPSKLIQFDPGMSTKMWNIAITYDGLEEDDEVFEVILNSPVNAVLGTKTKAAVKILDSKGGQCHPSYSSNQSKHSTWEKGIWHLLPPGSSSSTTSGSFHLERRPLPSSMQLAVIRGDTLRGFDSTDLSQRKLRTRGNGKTVRPSSVYRNGTDIIYNYHGIVSLKLEDDSFPTHKRKAKVSIISQPQKTIKVAELPQADKVESTTDSHFPRQDQLPSFPKNCTLELKGLFHFEEGIQKLYQCNGIAWKAWSPQTKDVEDKSCPAGWHQHSGYCHILITEQKGTWNAAAQACREQYLGNLVTVFSRQHMRWLWDIGGRKSFWIGLNDQVHAGHWEWIGGEPVAFTNGRRGPSQRSKLGKSCVLVQRQGKWQTKDCRRAKPHNYVCSRKL

Target ORF information:

RefSeq Version	XM_017014321.1
Organism	Homo sapiens(human)
Definition	Homo sapiens FRAS1 related extracellular matrix 1 (FREM1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017014321.1

ORF Insert Sequence:

ATGAACTCTC TGAGTTGGGG GGCTGCGAAT GCCGTGCTGC TGCTGCTCCT CCTGGCCTGG 
GCCAGCCCCA CCTTCATCAG CATCAACCGC GGGGTGAGGG TGATGAAGGG CCACTCTGCC 
TTCCTGTCAG GAGATGACCT GAAGTTTGCC ATCCCTAAAG AGAAAGATGC CTGCAAAGTG 
GAAGTTGTGA TGAATGAGCC AATAACCCAG AGGGTTGGGA AACTCACTCC ACAGGTCTTT 
GACTGCCATT TCCTTCCCAA CGAAGTCAAG TATGTTCACA ATGGTTGTCC AATTCTTGAT 
GAAGACACAG TGAAGCTCAG ACTTTACAGA TTTACTGAAA GAGATACCTT CATAGAAACT 
TTTATCCTGT GGGTCTATCT CCTGGAACCA GACTGTAACA TCATCCATAT GAGTAACAAT 
GTGCTGGAGG TGCCTGAATT CAATGGCTTG TCCCAAGCGA TTGATAAAAA TCTGCTCAGA 
TTCGATTATG ATAGGATGGC TAGCCTGGAA TGTACCGTCA GCCTGGACAC TGCGAGAACT 
CGGCTGCCAG CCCATGGCCA GATGGTTCTC GGGGAGCCTC GACCAGAAGA ACCTCGTGGA 
GATCAGCCAC ACAGTTTTTT CCCAGAGTCC CGTATGATGT CTTTTAATGC CTTTCTGGAA 
CTGAGAGCAA AACTGAAGTG TCCAGGTGGG AGCTGTACCC CAGGATTAAA GAAAATAGGA 
AGCCTCAAAG TGAGCTGTGA GGAGTTCCTG CTGATGGGCC TTCGTTATCA GCATCTGGAT 
CCCCCTTCAC CCAACATTGA TTATATCTCC ATCCAACTGG ACCTCACAGA TACCAGGAGC 
AAAATTGTGT ACAAGTCAGA GAGTGCGTGG CTGCCTGTCT ATATCAGAGC TGGAATTCCG 
AATCAGATTC CAAAGGCTGC ATTCATGGCC GTGTTTATTC TGGAAGTGGA TCAGTTCATC 
CTGACCTCCT TGACTACATC AGTTCTGGAC TGTGAAGAAG ATGAGACCCC TAAACCCTTG 
CTGGTGTTCA ACATTACTAA AGCCCCGCTC CAGGGCTATG TGACTCACCT GTTGGATCAC 
ACCAGACCAA TCTCCTCATT CACCTGGAAA GATCTCAGTG ACATGCAGAT CGCCTATCAG 
CCACCAAACA GCAGCCATTC TGAGAGGAGA CATGATGAGG TAGAATTGGA GGTATACGAC 
TTCTTCTTTG AAAGGAGTGC ACCTATGACA GTCCACATCT CCATCAGAAC AGCAGATACA 
AATGCCCCCC GTGTATCCTG GAATACAGGT CTGAGTCTCC TTGAGGGGCA GTCTCGAGCC 
ATCACTTGGG AACAGTTTCA GGTTGTCGAC AATGACGACA TTGGTGCTGT CCGGCTAGTC 
ACCGTTGGTG GCCTGCAGCA TGGATGGCTG ACTTTAAGAG GGGGGAAAGG GTTTCTCTTC 
ACCGTGGCTG ACCTCCAGGC TGGAGTTGTT CGCTATCATC ATGATGACAG CGACTCCACC 
AAAGACTTCG TGGTCTTCCG GATATTTGAT GGCCATCACA GCATCCGTCA CAAATTCCCC 
ATCAACGTCT TGCCCAAAGA TGATAGTCCC CCGTTCCTCA TAACCAATGT TGTGATTGAA 
CTGGAGGAGG GGCAGACCAT CCTGATCCAG GGATCCATGC TGCGAGCTTC AGATGTGGAC 
GCCAGTGATG ACTACATCTT CTTCAATATC ACAAAGCCTC CACAGGCTGG GGAGATCATG 
AAGAAGCCAG GGCCAGGACT GATAGGCTAT CCTGTCCATG GCTTCCTTCA GAGGGATTTG 
TTTAATGGAA TCATTTATTA TCGTCATTTT GGTGGAGAAA TCTTTGAAGA TTCTTTTCAA 
TTTGTCCTGT GGGACAGCCA TGAACCTCCA AATCTTTCAG TGCCACAGGT GGCAACAATC 
CATATAACTC CAGTGGATGA CCAGCTTCCA AAAGAGGCTC CTGGAGTTTC TCGGCATTTG 
GTTGTCAAGG AAACTGAGGT GGCCTATATA ACTAAGAAAC AGCTACATTT TATAGATTCA 
GAATCATATG ACAGGGAGCT GGTCTACACA ATAACTACTC CTCCATTTTT CTCCTTCAGC 
CACAGACACT TGGATGCTGG GAAATTATTT ATGGTGGACA GCATACCAAA AGTAGTTAAG 
AATCCTACGG CCCTGGAGCT GAGGTCATTC ACTCAGCATG CTGTGAACTA TATGAAAGTG 
GCCTACATGC CCCCCATGCA AGACATTGGT CCCCATTGCA GAGATGTCCA GTTCACATTT 
TCTGTCAGTA ACCAACATGG CGGTACTTTG CATGGGATCT GCTTTAACAT TACAATCCTC 
CCAGTGGACA ATCAAGTTCC TGAGGCGTTC ACCAACCCTC TGAAAGTGAC TGAGGGAGGT 
CAAAGCATCA TCAGCACAGA GCACATTCTA ATTTCTGATG CAGATACCAA GCTGGACAAT 
ATTGACCTCT CCCTGCGGGA ATTGCCTCTG CACGGAAGGG TGGAGCTGAA TGGATTTCCT 
CTAAATTCAG GGGGCACATT TTCTTGGGGC GATCTCCATA CCTTAAAAGT TAGGTATCAA 
CATGATGGAA CTGAAGTTCT TCAGGATGAC CTACTCTTGG AGGTCACCGA TGGCACAAAT 
TCAGCAGAAT TTGTACTACA TGTTGAGGTA TTCCCTGTCA ACGATGAGCC ACCAGTCTTA 
AAGGCTGACC TCATGCCTGT CATGAATTGC TCAGAGGGAG GAGAGGTGGT CATCACCTCT 
GAATACATTT TTGCTACTGA TGTGGACAGC GATAACTTGA AGTTGATGTT TGTGATTGCT 
CGCGAACCTC AGCATGGGGT GGTGAGGAGA GCTGGAGTCA CAGTGGATCA GTTCTCTCAG 
AGAGATGTTA TCTCAGAGGC CGTGACATAC AAACACACAG GTGGCGAGAT TGGCCTGATG 
CCTTGTTTTG ACACCATTAC ATTGGTGGTT TCGGATGGAG AGGCTGGCCC TTTTGTGAAT 
GGCTGTTGCT ACAATGGACC CAATCCATCT GTTCCACTTC ATGCGTCCTT TCCAGTGTAT 
GATCTCAACA TCACGGTATA CCCAGTAGAC AACCAGCCAC CTTCCATTGC AATAGGTCCC 
GTGTTTGTTG TAGATGAGGG CTGCTCAACA GCCCTTACTG TTAACCATTT GTCCGCCACT 
GACCCTGACA CTGCAGCAGA TGACTTGGAA TTTGTTTTGG TTTCTCCTCC TCAGTTTGGC 
TACCTCGAAA ATATACTCCC TTCTGTGGGT TTTGAAAAAA GCAATATTGG CATAAGTATA 
GATTCATTTC AGTGGAAAGA CATGAACGCT TTTCACATTA ACTATGTGCA GTCCAGGCAT 
CTGAGGATAG AACCAACTGC CGACCAGTTC ACGGTGTACG TCACAGATGG GAAGCATCAC 
TCCTTGGAGA TACCATTTTC TATTATAATC AACCCCACAA ATGATGAAGC TCCTGACTTT 
GTAGTGCAGA ATATTACTGT GTGTGAGGGT CAGATGAAAG AGCTGGACTC TTCCATCATC 
AGCGCTGTGG ACCTGGACAT TCCCCAGGAT GCCCTGCTGT TCAGCATCAC TCAAAAGCCA 
CGCCATGGCC TCCTCATCGA TAGGGGGTTT AGCAAAGACT TCTCTGAGAA TAAGCAGCCA 
GCCAACCCTC ACCAGAAACA TGCACCTGTT CACAGCTTTT CCATGGAACT CCTCAAGACT 
GGAATGAGGT TGACGTACAT GCATGATGAC TCAGAGAGCC TTGCTGATGA TTTTACAATC 
CAATTGTCAG ATGGGAAACA TAAGATACTT AAAACCATTT CAGTAGAGGT CATCCCAGTT 
AATGATGAAA AACCAATGCT GAGCAAGAAG GCTGAAATTG CAATGAATAT GGGTGAAACT 
CGTATTATTT CCAGTGCTAT TCTTTCAGCC ATAGATGAAG ACTCACCCAG GGAGAAGATT 
TACTATGTAT TTGAAAGGCT TCCCCAAAAT GGGCAACTTC AGCTTAAGAT AGGGAGGGAC 
TGGGTTCCTC TCTCCCCTGG CATGAAATGC ACTCAGGAGG AAGTGGATCT GAACTTGCTG 
AGATACACAC ACACCGGGGC AATGGATTCC CAGAATCAAG ATAGCTTCAC CTTCTACCTT 
TGGGATGGCA ACAACAGGTC CCCTGCTCTT GACTGTCAAA TCACCATCAA GGACATGGAA 
AAAGGTGATA TTGTCATCCT TACAAAACCA CTCGTGGTGT CTAAAGGTGA TAGAGGTTTC 
TTAACAACCA CCACGCTCCT GGCTGTGGAC GGAACAGACA AGCCTGAGGA ACTGCTCTAT 
GTCATCACCT CCCCTCCGCG ATATGGCCAG ATCGAATATG TTCACTATCC TGGAGTTCCC 
ATTACAAACT TCAGCCAAAT GGATGTAGTG GGGCAGACAG TTTGCTATGT ACACAAGAGC 
AAGGTGACTG TCTCCAGTGA CAGATTCAGA TTCATCATCA GCAATGGACT GCGGACCGAG 
CACGGGGTGT TTGAGATCAC ACTGGAGACT GTGGACAGAG CCCTGCCTGT GGTAACCAGG 
AACAAGGGGT TGAGACTGGC CCAAGGGGCC GTGGGCCTGC TTTCCCCTGA CCTCCTTCAG 
CTGACCGACC CTGATACACC TGCGGAGAAC CTCACCTTCC TCTTGGTTCA GCTCCCCCAG 
CATGGCCAGC TCTACCTGTG GGGGACAGGG CTACTTCAAC ACAATTTCAC CCAGCAGGAT 
GTGGACAGCA AGAATGTGGC CTATCGGCAC TCAGGAGGGG ACTCCCAGAC TGACTGCTTT 
ACTTTCATGG CCACAGATGG GACAAACCAA GGCTTTATTG TGAATGGGAG AGTGTGGGAA 
GAACCTGTTT TATTCACCAT TCAGGTAGAC CAATTGGACA AAACAGCTCC TCGTATCACA 
CTCTTGCATT CCCCTTCTCA AGTGGGGCTC CTGAAAAATG GCTGCTACGG GATTTACATC 
ACTTCCCGCG TGTTGAAGGC ATCAGACCCT GACACTGAGG ACGATCAGAT CATCTTTAAA 
ATTCTACAAG GCCCAAAACA TGGACATCTG GAGAACACAA CAACAGGTGA ATTTATCCAT 
GAGAAATTTA GCCAAAAGGA CTTAAACAGT AAGACTATTC TTTACATCAT AAACCCATCT 
TTGGAAGTAA ATTCAGATAC CGTGGAATTT CAAATCATGG ACCCCACAGG GAACTCGGCC 
ACTCCTCAAA TTTTGGAACT GAAGTGGTCT CATATTGAAT GGTCACAGAC CGAATATGAA 
GTCTGTGAGA ATGTGGGTTT GTTGCCCTTG GAAATTATCA GAAGGGGATA TTCCATGGAC 
TCGGCCTTTG TGGGTATAAA GGTCAACCAA GTGTCAGCTG CAGTTGGAAA AGATTTCACC 
GTGATTCCAT CTAAACTGAT TCAGTTTGAC CCAGGAATGT CAACTAAGAT GTGGAATATA 
GCAATTACCT ATGACGGATT AGAGGAAGAT GATGAGGTCT TTGAAGTAAT TCTGAACTCC 
CCTGTGAATG CAGTTCTTGG CACAAAGACA AAAGCTGCAG TGAAAATTTT GGACTCAAAA 
GGAGGACAAT GCCATCCTTC ATATTCCTCC AACCAAAGCA AGCACAGCAC ATGGGAGAAG 
GGCATTTGGC ATCTGCTGCC CCCAGGGTCT TCCTCATCCA CCACTTCTGG TTCCTTTCAT 
CTGGAAAGAA GACCTCTTCC ATCTTCCATG CAGCTAGCAG TCATCAGGGG AGACACCCTG 
CGGGGCTTTG ATTCTACAGA TCTTTCTCAA AGGAAGCTTA GGACCCGTGG GAATGGCAAA 
ACAGTTCGTC CATCCTCTGT TTATAGAAAT GGAACAGACA TCATCTATAA TTATCATGGG 
ATAGTTTCCT TGAAACTGGA GGATGACAGT TTCCCAACTC ACAAAAGGAA GGCCAAAGTA 
TCCATCATTA GTCAGCCACA AAAGACAATC AAAGTGGCAG AACTGCCTCA AGCAGATAAG 
GTGGAATCCA CAACTGACTC ACACTTCCCC AGACAGGACC AGTTGCCCTC ATTTCCAAAG 
AACTGCACTC TGGAATTAAA GGGACTCTTC CATTTTGAAG AAGGCATCCA GAAGCTGTAT 
CAGTGCAATG GGATCGCCTG GAAAGCCTGG AGTCCCCAAA CCAAGGATGT GGAAGACAAA 
TCCTGTCCAG CCGGGTGGCA CCAGCACTCA GGCTACTGTC ACATCTTGAT CACAGAGCAG 
AAAGGCACCT GGAATGCGGC TGCCCAAGCT TGCAGGGAAC AATACCTGGG CAACCTTGTA 
ACTGTATTCT CCAGGCAGCA CATGCGGTGG CTCTGGGACA TTGGTGGGAG AAAGTCCTTT 
TGGATAGGTT TGAACGACCA AGTGCATGCT GGCCACTGGG AGTGGATCGG TGGTGAACCT 
GTTGCCTTCA CCAATGGGAG AAGAGGGCCC TCTCAACGCT CCAAGCTTGG AAAGAGCTGT 
GTTTTGGTTC AAAGACAAGG GAAATGGCAA ACAAAAGACT GTAGGAGAGC CAAACCTCAT 
AATTATGTGT GTTCCAGAAA ACTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FREM1 ( XM_017014320.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016869809.1 Homo sapiens FRAS1 related extracellular matrix 1 (FREM1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OHu98459
Clone ID Related Accession (Same CDS sequence)	XM_017014320.1 , XM_017014318.1 , XM_017014316.1 , XM_017014316.2 , XM_017014317.1 , XM_017014319.2 , XM_017014321.2 , XM_017014323.1 , XM_017014322.1 , XM_017014321.1 , XM_017014320.2 , XM_017014319.1
Accession Version	XM_017014320.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6567bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	FRAS1-related extracellular matrix protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017014320.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACTCTC TGAGTTGGGG GGCTGCGAAT GCCGTGCTGC TGCTGCTCCT CCTGGCCTGG 
GCCAGCCCCA CCTTCATCAG CATCAACCGC GGGGTGAGGG TGATGAAGGG CCACTCTGCC 
TTCCTGTCAG GAGATGACCT GAAGTTTGCC ATCCCTAAAG AGAAAGATGC CTGCAAAGTG 
GAAGTTGTGA TGAATGAGCC AATAACCCAG AGGGTTGGGA AACTCACTCC ACAGGTCTTT 
GACTGCCATT TCCTTCCCAA CGAAGTCAAG TATGTTCACA ATGGTTGTCC AATTCTTGAT 
GAAGACACAG TGAAGCTCAG ACTTTACAGA TTTACTGAAA GAGATACCTT CATAGAAACT 
TTTATCCTGT GGGTCTATCT CCTGGAACCA GACTGTAACA TCATCCATAT GAGTAACAAT 
GTGCTGGAGG TGCCTGAATT CAATGGCTTG TCCCAAGCGA TTGATAAAAA TCTGCTCAGA 
TTCGATTATG ATAGGATGGC TAGCCTGGAA TGTACCGTCA GCCTGGACAC TGCGAGAACT 
CGGCTGCCAG CCCATGGCCA GATGGTTCTC GGGGAGCCTC GACCAGAAGA ACCTCGTGGA 
GATCAGCCAC ACAGTTTTTT CCCAGAGTCC CGTATGATGT CTTTTAATGC CTTTCTGGAA 
CTGAGAGCAA AACTGAAGTG TCCAGGTGGG AGCTGTACCC CAGGATTAAA GAAAATAGGA 
AGCCTCAAAG TGAGCTGTGA GGAGTTCCTG CTGATGGGCC TTCGTTATCA GCATCTGGAT 
CCCCCTTCAC CCAACATTGA TTATATCTCC ATCCAACTGG ACCTCACAGA TACCAGGAGC 
AAAATTGTGT ACAAGTCAGA GAGTGCGTGG CTGCCTGTCT ATATCAGAGC TGGAATTCCG 
AATCAGATTC CAAAGGCTGC ATTCATGGCC GTGTTTATTC TGGAAGTGGA TCAGTTCATC 
CTGACCTCCT TGACTACATC AGTTCTGGAC TGTGAAGAAG ATGAGACCCC TAAACCCTTG 
CTGGTGTTCA ACATTACTAA AGCCCCGCTC CAGGGCTATG TGACTCACCT GTTGGATCAC 
ACCAGACCAA TCTCCTCATT CACCTGGAAA GATCTCAGTG ACATGCAGAT CGCCTATCAG 
CCACCAAACA GCAGCCATTC TGAGAGGAGA CATGATGAGG TAGAATTGGA GGTATACGAC 
TTCTTCTTTG AAAGGAGTGC ACCTATGACA GTCCACATCT CCATCAGAAC AGCAGATACA 
AATGCCCCCC GTGTATCCTG GAATACAGGT CTGAGTCTCC TTGAGGGGCA GTCTCGAGCC 
ATCACTTGGG AACAGTTTCA GGTTGTCGAC AATGACGACA TTGGTGCTGT CCGGCTAGTC 
ACCGTTGGTG GCCTGCAGCA TGGATGGCTG ACTTTAAGAG GGGGGAAAGG GTTTCTCTTC 
ACCGTGGCTG ACCTCCAGGC TGGAGTTGTT CGCTATCATC ATGATGACAG CGACTCCACC 
AAAGACTTCG TGGTCTTCCG GATATTTGAT GGCCATCACA GCATCCGTCA CAAATTCCCC 
ATCAACGTCT TGCCCAAAGA TGATAGTCCC CCGTTCCTCA TAACCAATGT TGTGATTGAA 
CTGGAGGAGG GGCAGACCAT CCTGATCCAG GGATCCATGC TGCGAGCTTC AGATGTGGAC 
GCCAGTGATG ACTACATCTT CTTCAATATC ACAAAGCCTC CACAGGCTGG GGAGATCATG 
AAGAAGCCAG GGCCAGGACT GATAGGCTAT CCTGTCCATG GCTTCCTTCA GAGGGATTTG 
TTTAATGGAA TCATTTATTA TCGTCATTTT GGTGGAGAAA TCTTTGAAGA TTCTTTTCAA 
TTTGTCCTGT GGGACAGCCA TGAACCTCCA AATCTTTCAG TGCCACAGGT GGCAACAATC 
CATATAACTC CAGTGGATGA CCAGCTTCCA AAAGAGGCTC CTGGAGTTTC TCGGCATTTG 
GTTGTCAAGG AAACTGAGGT GGCCTATATA ACTAAGAAAC AGCTACATTT TATAGATTCA 
GAATCATATG ACAGGGAGCT GGTCTACACA ATAACTACTC CTCCATTTTT CTCCTTCAGC 
CACAGACACT TGGATGCTGG GAAATTATTT ATGGTGGACA GCATACCAAA AGTAGTTAAG 
AATCCTACGG CCCTGGAGCT GAGGTCATTC ACTCAGCATG CTGTGAACTA TATGAAAGTG 
GCCTACATGC CCCCCATGCA AGACATTGGT CCCCATTGCA GAGATGTCCA GTTCACATTT 
TCTGTCAGTA ACCAACATGG CGGTACTTTG CATGGGATCT GCTTTAACAT TACAATCCTC 
CCAGTGGACA ATCAAGTTCC TGAGGCGTTC ACCAACCCTC TGAAAGTGAC TGAGGGAGGT 
CAAAGCATCA TCAGCACAGA GCACATTCTA ATTTCTGATG CAGATACCAA GCTGGACAAT 
ATTGACCTCT CCCTGCGGGA ATTGCCTCTG CACGGAAGGG TGGAGCTGAA TGGATTTCCT 
CTAAATTCAG GGGGCACATT TTCTTGGGGC GATCTCCATA CCTTAAAAGT TAGGTATCAA 
CATGATGGAA CTGAAGTTCT TCAGGATGAC CTACTCTTGG AGGTCACCGA TGGCACAAAT 
TCAGCAGAAT TTGTACTACA TGTTGAGGTA TTCCCTGTCA ACGATGAGCC ACCAGTCTTA 
AAGGCTGACC TCATGCCTGT CATGAATTGC TCAGAGGGAG GAGAGGTGGT CATCACCTCT 
GAATACATTT TTGCTACTGA TGTGGACAGC GATAACTTGA AGTTGATGTT TGTGATTGCT 
CGCGAACCTC AGCATGGGGT GGTGAGGAGA GCTGGAGTCA CAGTGGATCA GTTCTCTCAG 
AGAGATGTTA TCTCAGAGGC CGTGACATAC AAACACACAG GTGGCGAGAT TGGCCTGATG 
CCTTGTTTTG ACACCATTAC ATTGGTGGTT TCGGATGGAG AGGCTGGCCC TTTTGTGAAT 
GGCTGTTGCT ACAATGGACC CAATCCATCT GTTCCACTTC ATGCGTCCTT TCCAGTGTAT 
GATCTCAACA TCACGGTATA CCCAGTAGAC AACCAGCCAC CTTCCATTGC AATAGGTCCC 
GTGTTTGTTG TAGATGAGGG CTGCTCAACA GCCCTTACTG TTAACCATTT GTCCGCCACT 
GACCCTGACA CTGCAGCAGA TGACTTGGAA TTTGTTTTGG TTTCTCCTCC TCAGTTTGGC 
TACCTCGAAA ATATACTCCC TTCTGTGGGT TTTGAAAAAA GCAATATTGG CATAAGTATA 
GATTCATTTC AGTGGAAAGA CATGAACGCT TTTCACATTA ACTATGTGCA GTCCAGGCAT 
CTGAGGATAG AACCAACTGC CGACCAGTTC ACGGTGTACG TCACAGATGG GAAGCATCAC 
TCCTTGGAGA TACCATTTTC TATTATAATC AACCCCACAA ATGATGAAGC TCCTGACTTT 
GTAGTGCAGA ATATTACTGT GTGTGAGGGT CAGATGAAAG AGCTGGACTC TTCCATCATC 
AGCGCTGTGG ACCTGGACAT TCCCCAGGAT GCCCTGCTGT TCAGCATCAC TCAAAAGCCA 
CGCCATGGCC TCCTCATCGA TAGGGGGTTT AGCAAAGACT TCTCTGAGAA TAAGCAGCCA 
GCCAACCCTC ACCAGAAACA TGCACCTGTT CACAGCTTTT CCATGGAACT CCTCAAGACT 
GGAATGAGGT TGACGTACAT GCATGATGAC TCAGAGAGCC TTGCTGATGA TTTTACAATC 
CAATTGTCAG ATGGGAAACA TAAGATACTT AAAACCATTT CAGTAGAGGT CATCCCAGTT 
AATGATGAAA AACCAATGCT GAGCAAGAAG GCTGAAATTG CAATGAATAT GGGTGAAACT 
CGTATTATTT CCAGTGCTAT TCTTTCAGCC ATAGATGAAG ACTCACCCAG GGAGAAGATT 
TACTATGTAT TTGAAAGGCT TCCCCAAAAT GGGCAACTTC AGCTTAAGAT AGGGAGGGAC 
TGGGTTCCTC TCTCCCCTGG CATGAAATGC ACTCAGGAGG AAGTGGATCT GAACTTGCTG 
AGATACACAC ACACCGGGGC AATGGATTCC CAGAATCAAG ATAGCTTCAC CTTCTACCTT 
TGGGATGGCA ACAACAGGTC CCCTGCTCTT GACTGTCAAA TCACCATCAA GGACATGGAA 
AAAGGTGATA TTGTCATCCT TACAAAACCA CTCGTGGTGT CTAAAGGTGA TAGAGGTTTC 
TTAACAACCA CCACGCTCCT GGCTGTGGAC GGAACAGACA AGCCTGAGGA ACTGCTCTAT 
GTCATCACCT CCCCTCCGCG ATATGGCCAG ATCGAATATG TTCACTATCC TGGAGTTCCC 
ATTACAAACT TCAGCCAAAT GGATGTAGTG GGGCAGACAG TTTGCTATGT ACACAAGAGC 
AAGGTGACTG TCTCCAGTGA CAGATTCAGA TTCATCATCA GCAATGGACT GCGGACCGAG 
CACGGGGTGT TTGAGATCAC ACTGGAGACT GTGGACAGAG CCCTGCCTGT GGTAACCAGG 
AACAAGGGGT TGAGACTGGC CCAAGGGGCC GTGGGCCTGC TTTCCCCTGA CCTCCTTCAG 
CTGACCGACC CTGATACACC TGCGGAGAAC CTCACCTTCC TCTTGGTTCA GCTCCCCCAG 
CATGGCCAGC TCTACCTGTG GGGGACAGGG CTACTTCAAC ACAATTTCAC CCAGCAGGAT 
GTGGACAGCA AGAATGTGGC CTATCGGCAC TCAGGAGGGG ACTCCCAGAC TGACTGCTTT 
ACTTTCATGG CCACAGATGG GACAAACCAA GGCTTTATTG TGAATGGGAG AGTGTGGGAA 
GAACCTGTTT TATTCACCAT TCAGGTAGAC CAATTGGACA AAACAGCTCC TCGTATCACA 
CTCTTGCATT CCCCTTCTCA AGTGGGGCTC CTGAAAAATG GCTGCTACGG GATTTACATC 
ACTTCCCGCG TGTTGAAGGC ATCAGACCCT GACACTGAGG ACGATCAGAT CATCTTTAAA 
ATTCTACAAG GCCCAAAACA TGGACATCTG GAGAACACAA CAACAGGTGA ATTTATCCAT 
GAGAAATTTA GCCAAAAGGA CTTAAACAGT AAGACTATTC TTTACATCAT AAACCCATCT 
TTGGAAGTAA ATTCAGATAC CGTGGAATTT CAAATCATGG ACCCCACAGG GAACTCGGCC 
ACTCCTCAAA TTTTGGAACT GAAGTGGTCT CATATTGAAT GGTCACAGAC CGAATATGAA 
GTCTGTGAGA ATGTGGGTTT GTTGCCCTTG GAAATTATCA GAAGGGGATA TTCCATGGAC 
TCGGCCTTTG TGGGTATAAA GGTCAACCAA GTGTCAGCTG CAGTTGGAAA AGATTTCACC 
GTGATTCCAT CTAAACTGAT TCAGTTTGAC CCAGGAATGT CAACTAAGAT GTGGAATATA 
GCAATTACCT ATGACGGATT AGAGGAAGAT GATGAGGTCT TTGAAGTAAT TCTGAACTCC 
CCTGTGAATG CAGTTCTTGG CACAAAGACA AAAGCTGCAG TGAAAATTTT GGACTCAAAA 
GGAGGACAAT GCCATCCTTC ATATTCCTCC AACCAAAGCA AGCACAGCAC ATGGGAGAAG 
GGCATTTGGC ATCTGCTGCC CCCAGGGTCT TCCTCATCCA CCACTTCTGG TTCCTTTCAT 
CTGGAAAGAA GACCTCTTCC ATCTTCCATG CAGCTAGCAG TCATCAGGGG AGACACCCTG 
CGGGGCTTTG ATTCTACAGA TCTTTCTCAA AGGAAGCTTA GGACCCGTGG GAATGGCAAA 
ACAGTTCGTC CATCCTCTGT TTATAGAAAT GGAACAGACA TCATCTATAA TTATCATGGG 
ATAGTTTCCT TGAAACTGGA GGATGACAGT TTCCCAACTC ACAAAAGGAA GGCCAAAGTA 
TCCATCATTA GTCAGCCACA AAAGACAATC AAAGTGGCAG AACTGCCTCA AGCAGATAAG 
GTGGAATCCA CAACTGACTC ACACTTCCCC AGACAGGACC AGTTGCCCTC ATTTCCAAAG 
AACTGCACTC TGGAATTAAA GGGACTCTTC CATTTTGAAG AAGGCATCCA GAAGCTGTAT 
CAGTGCAATG GGATCGCCTG GAAAGCCTGG AGTCCCCAAA CCAAGGATGT GGAAGACAAA 
TCCTGTCCAG CCGGGTGGCA CCAGCACTCA GGCTACTGTC ACATCTTGAT CACAGAGCAG 
AAAGGCACCT GGAATGCGGC TGCCCAAGCT TGCAGGGAAC AATACCTGGG CAACCTTGTA 
ACTGTATTCT CCAGGCAGCA CATGCGGTGG CTCTGGGACA TTGGTGGGAG AAAGTCCTTT 
TGGATAGGTT TGAACGACCA AGTGCATGCT GGCCACTGGG AGTGGATCGG TGGTGAACCT 
GTTGCCTTCA CCAATGGGAG AAGAGGGCCC TCTCAACGCT CCAAGCTTGG AAAGAGCTGT 
GTTTTGGTTC AAAGACAAGG GAAATGGCAA ACAAAAGACT GTAGGAGAGC CAAACCTCAT 
AATTATGTGT GTTCCAGAAA ACTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016869809.1
CDS	1189..7755
Translation	MNSLSWGAANAVLLLLLLAWASPTFISINRGVRVMKGHSAFLSGDDLKFAIPKEKDACKVEVVMNEPITQRVGKLTPQVFDCHFLPNEVKYVHNGCPILDEDTVKLRLYRFTERDTFIETFILWVYLLEPDCNIIHMSNNVLEVPEFNGLSQAIDKNLLRFDYDRMASLECTVSLDTARTRLPAHGQMVLGEPRPEEPRGDQPHSFFPESRMMSFNAFLELRAKLKCPGGSCTPGLKKIGSLKVSCEEFLLMGLRYQHLDPPSPNIDYISIQLDLTDTRSKIVYKSESAWLPVYIRAGIPNQIPKAAFMAVFILEVDQFILTSLTTSVLDCEEDETPKPLLVFNITKAPLQGYVTHLLDHTRPISSFTWKDLSDMQIAYQPPNSSHSERRHDEVELEVYDFFFERSAPMTVHISIRTADTNAPRVSWNTGLSLLEGQSRAITWEQFQVVDNDDIGAVRLVTVGGLQHGWLTLRGGKGFLFTVADLQAGVVRYHHDDSDSTKDFVVFRIFDGHHSIRHKFPINVLPKDDSPPFLITNVVIELEEGQTILIQGSMLRASDVDASDDYIFFNITKPPQAGEIMKKPGPGLIGYPVHGFLQRDLFNGIIYYRHFGGEIFEDSFQFVLWDSHEPPNLSVPQVATIHITPVDDQLPKEAPGVSRHLVVKETEVAYITKKQLHFIDSESYDRELVYTITTPPFFSFSHRHLDAGKLFMVDSIPKVVKNPTALELRSFTQHAVNYMKVAYMPPMQDIGPHCRDVQFTFSVSNQHGGTLHGICFNITILPVDNQVPEAFTNPLKVTEGGQSIISTEHILISDADTKLDNIDLSLRELPLHGRVELNGFPLNSGGTFSWGDLHTLKVRYQHDGTEVLQDDLLLEVTDGTNSAEFVLHVEVFPVNDEPPVLKADLMPVMNCSEGGEVVITSEYIFATDVDSDNLKLMFVIAREPQHGVVRRAGVTVDQFSQRDVISEAVTYKHTGGEIGLMPCFDTITLVVSDGEAGPFVNGCCYNGPNPSVPLHASFPVYDLNITVYPVDNQPPSIAIGPVFVVDEGCSTALTVNHLSATDPDTAADDLEFVLVSPPQFGYLENILPSVGFEKSNIGISIDSFQWKDMNAFHINYVQSRHLRIEPTADQFTVYVTDGKHHSLEIPFSIIINPTNDEAPDFVVQNITVCEGQMKELDSSIISAVDLDIPQDALLFSITQKPRHGLLIDRGFSKDFSENKQPANPHQKHAPVHSFSMELLKTGMRLTYMHDDSESLADDFTIQLSDGKHKILKTISVEVIPVNDEKPMLSKKAEIAMNMGETRIISSAILSAIDEDSPREKIYYVFERLPQNGQLQLKIGRDWVPLSPGMKCTQEEVDLNLLRYTHTGAMDSQNQDSFTFYLWDGNNRSPALDCQITIKDMEKGDIVILTKPLVVSKGDRGFLTTTTLLAVDGTDKPEELLYVITSPPRYGQIEYVHYPGVPITNFSQMDVVGQTVCYVHKSKVTVSSDRFRFIISNGLRTEHGVFEITLETVDRALPVVTRNKGLRLAQGAVGLLSPDLLQLTDPDTPAENLTFLLVQLPQHGQLYLWGTGLLQHNFTQQDVDSKNVAYRHSGGDSQTDCFTFMATDGTNQGFIVNGRVWEEPVLFTIQVDQLDKTAPRITLLHSPSQVGLLKNGCYGIYITSRVLKASDPDTEDDQIIFKILQGPKHGHLENTTTGEFIHEKFSQKDLNSKTILYIINPSLEVNSDTVEFQIMDPTGNSATPQILELKWSHIEWSQTEYEVCENVGLLPLEIIRRGYSMDSAFVGIKVNQVSAAVGKDFTVIPSKLIQFDPGMSTKMWNIAITYDGLEEDDEVFEVILNSPVNAVLGTKTKAAVKILDSKGGQCHPSYSSNQSKHSTWEKGIWHLLPPGSSSSTTSGSFHLERRPLPSSMQLAVIRGDTLRGFDSTDLSQRKLRTRGNGKTVRPSSVYRNGTDIIYNYHGIVSLKLEDDSFPTHKRKAKVSIISQPQKTIKVAELPQADKVESTTDSHFPRQDQLPSFPKNCTLELKGLFHFEEGIQKLYQCNGIAWKAWSPQTKDVEDKSCPAGWHQHSGYCHILITEQKGTWNAAAQACREQYLGNLVTVFSRQHMRWLWDIGGRKSFWIGLNDQVHAGHWEWIGGEPVAFTNGRRGPSQRSKLGKSCVLVQRQGKWQTKDCRRAKPHNYVCSRKL

Target ORF information:

RefSeq Version	XM_017014320.2
Organism	Homo sapiens(human)
Definition	Homo sapiens FRAS1 related extracellular matrix 1 (FREM1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017014320.2

ORF Insert Sequence:

ATGAACTCTC TGAGTTGGGG GGCTGCGAAT GCCGTGCTGC TGCTGCTCCT CCTGGCCTGG 
GCCAGCCCCA CCTTCATCAG CATCAACCGC GGGGTGAGGG TGATGAAGGG CCACTCTGCC 
TTCCTGTCAG GAGATGACCT GAAGTTTGCC ATCCCTAAAG AGAAAGATGC CTGCAAAGTG 
GAAGTTGTGA TGAATGAGCC AATAACCCAG AGGGTTGGGA AACTCACTCC ACAGGTCTTT 
GACTGCCATT TCCTTCCCAA CGAAGTCAAG TATGTTCACA ATGGTTGTCC AATTCTTGAT 
GAAGACACAG TGAAGCTCAG ACTTTACAGA TTTACTGAAA GAGATACCTT CATAGAAACT 
TTTATCCTGT GGGTCTATCT CCTGGAACCA GACTGTAACA TCATCCATAT GAGTAACAAT 
GTGCTGGAGG TGCCTGAATT CAATGGCTTG TCCCAAGCGA TTGATAAAAA TCTGCTCAGA 
TTCGATTATG ATAGGATGGC TAGCCTGGAA TGTACCGTCA GCCTGGACAC TGCGAGAACT 
CGGCTGCCAG CCCATGGCCA GATGGTTCTC GGGGAGCCTC GACCAGAAGA ACCTCGTGGA 
GATCAGCCAC ACAGTTTTTT CCCAGAGTCC CGTATGATGT CTTTTAATGC CTTTCTGGAA 
CTGAGAGCAA AACTGAAGTG TCCAGGTGGG AGCTGTACCC CAGGATTAAA GAAAATAGGA 
AGCCTCAAAG TGAGCTGTGA GGAGTTCCTG CTGATGGGCC TTCGTTATCA GCATCTGGAT 
CCCCCTTCAC CCAACATTGA TTATATCTCC ATCCAACTGG ACCTCACAGA TACCAGGAGC 
AAAATTGTGT ACAAGTCAGA GAGTGCGTGG CTGCCTGTCT ATATCAGAGC TGGAATTCCG 
AATCAGATTC CAAAGGCTGC ATTCATGGCC GTGTTTATTC TGGAAGTGGA TCAGTTCATC 
CTGACCTCCT TGACTACATC AGTTCTGGAC TGTGAAGAAG ATGAGACCCC TAAACCCTTG 
CTGGTGTTCA ACATTACTAA AGCCCCGCTC CAGGGCTATG TGACTCACCT GTTGGATCAC 
ACCAGACCAA TCTCCTCATT CACCTGGAAA GATCTCAGTG ACATGCAGAT CGCCTATCAG 
CCACCAAACA GCAGCCATTC TGAGAGGAGA CATGATGAGG TAGAATTGGA GGTATACGAC 
TTCTTCTTTG AAAGGAGTGC ACCTATGACA GTCCACATCT CCATCAGAAC AGCAGATACA 
AATGCCCCCC GTGTATCCTG GAATACAGGT CTGAGTCTCC TTGAGGGGCA GTCTCGAGCC 
ATCACTTGGG AACAGTTTCA GGTTGTCGAC AATGACGACA TTGGTGCTGT CCGGCTAGTC 
ACCGTTGGTG GCCTGCAGCA TGGATGGCTG ACTTTAAGAG GGGGGAAAGG GTTTCTCTTC 
ACCGTGGCTG ACCTCCAGGC TGGAGTTGTT CGCTATCATC ATGATGACAG CGACTCCACC 
AAAGACTTCG TGGTCTTCCG GATATTTGAT GGCCATCACA GCATCCGTCA CAAATTCCCC 
ATCAACGTCT TGCCCAAAGA TGATAGTCCC CCGTTCCTCA TAACCAATGT TGTGATTGAA 
CTGGAGGAGG GGCAGACCAT CCTGATCCAG GGATCCATGC TGCGAGCTTC AGATGTGGAC 
GCCAGTGATG ACTACATCTT CTTCAATATC ACAAAGCCTC CACAGGCTGG GGAGATCATG 
AAGAAGCCAG GGCCAGGACT GATAGGCTAT CCTGTCCATG GCTTCCTTCA GAGGGATTTG 
TTTAATGGAA TCATTTATTA TCGTCATTTT GGTGGAGAAA TCTTTGAAGA TTCTTTTCAA 
TTTGTCCTGT GGGACAGCCA TGAACCTCCA AATCTTTCAG TGCCACAGGT GGCAACAATC 
CATATAACTC CAGTGGATGA CCAGCTTCCA AAAGAGGCTC CTGGAGTTTC TCGGCATTTG 
GTTGTCAAGG AAACTGAGGT GGCCTATATA ACTAAGAAAC AGCTACATTT TATAGATTCA 
GAATCATATG ACAGGGAGCT GGTCTACACA ATAACTACTC CTCCATTTTT CTCCTTCAGC 
CACAGACACT TGGATGCTGG GAAATTATTT ATGGTGGACA GCATACCAAA AGTAGTTAAG 
AATCCTACGG CCCTGGAGCT GAGGTCATTC ACTCAGCATG CTGTGAACTA TATGAAAGTG 
GCCTACATGC CCCCCATGCA AGACATTGGT CCCCATTGCA GAGATGTCCA GTTCACATTT 
TCTGTCAGTA ACCAACATGG CGGTACTTTG CATGGGATCT GCTTTAACAT TACAATCCTC 
CCAGTGGACA ATCAAGTTCC TGAGGCGTTC ACCAACCCTC TGAAAGTGAC TGAGGGAGGT 
CAAAGCATCA TCAGCACAGA GCACATTCTA ATTTCTGATG CAGATACCAA GCTGGACAAT 
ATTGACCTCT CCCTGCGGGA ATTGCCTCTG CACGGAAGGG TGGAGCTGAA TGGATTTCCT 
CTAAATTCAG GGGGCACATT TTCTTGGGGC GATCTCCATA CCTTAAAAGT TAGGTATCAA 
CATGATGGAA CTGAAGTTCT TCAGGATGAC CTACTCTTGG AGGTCACCGA TGGCACAAAT 
TCAGCAGAAT TTGTACTACA TGTTGAGGTA TTCCCTGTCA ACGATGAGCC ACCAGTCTTA 
AAGGCTGACC TCATGCCTGT CATGAATTGC TCAGAGGGAG GAGAGGTGGT CATCACCTCT 
GAATACATTT TTGCTACTGA TGTGGACAGC GATAACTTGA AGTTGATGTT TGTGATTGCT 
CGCGAACCTC AGCATGGGGT GGTGAGGAGA GCTGGAGTCA CAGTGGATCA GTTCTCTCAG 
AGAGATGTTA TCTCAGAGGC CGTGACATAC AAACACACAG GTGGCGAGAT TGGCCTGATG 
CCTTGTTTTG ACACCATTAC ATTGGTGGTT TCGGATGGAG AGGCTGGCCC TTTTGTGAAT 
GGCTGTTGCT ACAATGGACC CAATCCATCT GTTCCACTTC ATGCGTCCTT TCCAGTGTAT 
GATCTCAACA TCACGGTATA CCCAGTAGAC AACCAGCCAC CTTCCATTGC AATAGGTCCC 
GTGTTTGTTG TAGATGAGGG CTGCTCAACA GCCCTTACTG TTAACCATTT GTCCGCCACT 
GACCCTGACA CTGCAGCAGA TGACTTGGAA TTTGTTTTGG TTTCTCCTCC TCAGTTTGGC 
TACCTCGAAA ATATACTCCC TTCTGTGGGT TTTGAAAAAA GCAATATTGG CATAAGTATA 
GATTCATTTC AGTGGAAAGA CATGAACGCT TTTCACATTA ACTATGTGCA GTCCAGGCAT 
CTGAGGATAG AACCAACTGC CGACCAGTTC ACGGTGTACG TCACAGATGG GAAGCATCAC 
TCCTTGGAGA TACCATTTTC TATTATAATC AACCCCACAA ATGATGAAGC TCCTGACTTT 
GTAGTGCAGA ATATTACTGT GTGTGAGGGT CAGATGAAAG AGCTGGACTC TTCCATCATC 
AGCGCTGTGG ACCTGGACAT TCCCCAGGAT GCCCTGCTGT TCAGCATCAC TCAAAAGCCA 
CGCCATGGCC TCCTCATCGA TAGGGGGTTT AGCAAAGACT TCTCTGAGAA TAAGCAGCCA 
GCCAACCCTC ACCAGAAACA TGCACCTGTT CACAGCTTTT CCATGGAACT CCTCAAGACT 
GGAATGAGGT TGACGTACAT GCATGATGAC TCAGAGAGCC TTGCTGATGA TTTTACAATC 
CAATTGTCAG ATGGGAAACA TAAGATACTT AAAACCATTT CAGTAGAGGT CATCCCAGTT 
AATGATGAAA AACCAATGCT GAGCAAGAAG GCTGAAATTG CAATGAATAT GGGTGAAACT 
CGTATTATTT CCAGTGCTAT TCTTTCAGCC ATAGATGAAG ACTCACCCAG GGAGAAGATT 
TACTATGTAT TTGAAAGGCT TCCCCAAAAT GGGCAACTTC AGCTTAAGAT AGGGAGGGAC 
TGGGTTCCTC TCTCCCCTGG CATGAAATGC ACTCAGGAGG AAGTGGATCT GAACTTGCTG 
AGATACACAC ACACCGGGGC AATGGATTCC CAGAATCAAG ATAGCTTCAC CTTCTACCTT 
TGGGATGGCA ACAACAGGTC CCCTGCTCTT GACTGTCAAA TCACCATCAA GGACATGGAA 
AAAGGTGATA TTGTCATCCT TACAAAACCA CTCGTGGTGT CTAAAGGTGA TAGAGGTTTC 
TTAACAACCA CCACGCTCCT GGCTGTGGAC GGAACAGACA AGCCTGAGGA ACTGCTCTAT 
GTCATCACCT CCCCTCCGCG ATATGGCCAG ATCGAATATG TTCACTATCC TGGAGTTCCC 
ATTACAAACT TCAGCCAAAT GGATGTAGTG GGGCAGACAG TTTGCTATGT ACACAAGAGC 
AAGGTGACTG TCTCCAGTGA CAGATTCAGA TTCATCATCA GCAATGGACT GCGGACCGAG 
CACGGGGTGT TTGAGATCAC ACTGGAGACT GTGGACAGAG CCCTGCCTGT GGTAACCAGG 
AACAAGGGGT TGAGACTGGC CCAAGGGGCC GTGGGCCTGC TTTCCCCTGA CCTCCTTCAG 
CTGACCGACC CTGATACACC TGCGGAGAAC CTCACCTTCC TCTTGGTTCA GCTCCCCCAG 
CATGGCCAGC TCTACCTGTG GGGGACAGGG CTACTTCAAC ACAATTTCAC CCAGCAGGAT 
GTGGACAGCA AGAATGTGGC CTATCGGCAC TCAGGAGGGG ACTCCCAGAC TGACTGCTTT 
ACTTTCATGG CCACAGATGG GACAAACCAA GGCTTTATTG TGAATGGGAG AGTGTGGGAA 
GAACCTGTTT TATTCACCAT TCAGGTAGAC CAATTGGACA AAACAGCTCC TCGTATCACA 
CTCTTGCATT CCCCTTCTCA AGTGGGGCTC CTGAAAAATG GCTGCTACGG GATTTACATC 
ACTTCCCGCG TGTTGAAGGC ATCAGACCCT GACACTGAGG ACGATCAGAT CATCTTTAAA 
ATTCTACAAG GCCCAAAACA TGGACATCTG GAGAACACAA CAACAGGTGA ATTTATCCAT 
GAGAAATTTA GCCAAAAGGA CTTAAACAGT AAGACTATTC TTTACATCAT AAACCCATCT 
TTGGAAGTAA ATTCAGATAC CGTGGAATTT CAAATCATGG ACCCCACAGG GAACTCGGCC 
ACTCCTCAAA TTTTGGAACT GAAGTGGTCT CATATTGAAT GGTCACAGAC CGAATATGAA 
GTCTGTGAGA ATGTGGGTTT GTTGCCCTTG GAAATTATCA GAAGGGGATA TTCCATGGAC 
TCGGCCTTTG TGGGTATAAA GGTCAACCAA GTGTCAGCTG CAGTTGGAAA AGATTTCACC 
GTGATTCCAT CTAAACTGAT TCAGTTTGAC CCAGGAATGT CAACTAAGAT GTGGAATATA 
GCAATTACCT ATGACGGATT AGAGGAAGAT GATGAGGTCT TTGAAGTAAT TCTGAACTCC 
CCTGTGAATG CAGTTCTTGG CACAAAGACA AAAGCTGCAG TGAAAATTTT GGACTCAAAA 
GGAGGACAAT GCCATCCTTC ATATTCCTCC AACCAAAGCA AGCACAGCAC ATGGGAGAAG 
GGCATTTGGC ATCTGCTGCC CCCAGGGTCT TCCTCATCCA CCACTTCTGG TTCCTTTCAT 
CTGGAAAGAA GACCTCTTCC ATCTTCCATG CAGCTAGCAG TCATCAGGGG AGACACCCTG 
CGGGGCTTTG ATTCTACAGA TCTTTCTCAA AGGAAGCTTA GGACCCGTGG GAATGGCAAA 
ACAGTTCGTC CATCCTCTGT TTATAGAAAT GGAACAGACA TCATCTATAA TTATCATGGG 
ATAGTTTCCT TGAAACTGGA GGATGACAGT TTCCCAACTC ACAAAAGGAA GGCCAAAGTA 
TCCATCATTA GTCAGCCACA AAAGACAATC AAAGTGGCAG AACTGCCTCA AGCAGATAAG 
GTGGAATCCA CAACTGACTC ACACTTCCCC AGACAGGACC AGTTGCCCTC ATTTCCAAAG 
AACTGCACTC TGGAATTAAA GGGACTCTTC CATTTTGAAG AAGGCATCCA GAAGCTGTAT 
CAGTGCAATG GGATCGCCTG GAAAGCCTGG AGTCCCCAAA CCAAGGATGT GGAAGACAAA 
TCCTGTCCAG CCGGGTGGCA CCAGCACTCA GGCTACTGTC ACATCTTGAT CACAGAGCAG 
AAAGGCACCT GGAATGCGGC TGCCCAAGCT TGCAGGGAAC AATACCTGGG CAACCTTGTA 
ACTGTATTCT CCAGGCAGCA CATGCGGTGG CTCTGGGACA TTGGTGGGAG AAAGTCCTTT 
TGGATAGGTT TGAACGACCA AGTGCATGCT GGCCACTGGG AGTGGATCGG TGGTGAACCT 
GTTGCCTTCA CCAATGGGAG AAGAGGGCCC TCTCAACGCT CCAAGCTTGG AAAGAGCTGT 
GTTTTGGTTC AAAGACAAGG GAAATGGCAA ACAAAAGACT GTAGGAGAGC CAAACCTCAT 
AATTATGTGT GTTCCAGAAA ACTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FREM1 ( XM_017014319.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016869808.1 Homo sapiens FRAS1 related extracellular matrix 1 (FREM1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OHu98459
Clone ID Related Accession (Same CDS sequence)	XM_017014320.1 , XM_017014318.1 , XM_017014316.1 , XM_017014316.2 , XM_017014317.1 , XM_017014319.2 , XM_017014321.2 , XM_017014323.1 , XM_017014322.1 , XM_017014321.1 , XM_017014320.2 , XM_017014319.1
Accession Version	XM_017014319.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6567bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	FRAS1-related extracellular matrix protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACTCTC TGAGTTGGGG GGCTGCGAAT GCCGTGCTGC TGCTGCTCCT CCTGGCCTGG 
GCCAGCCCCA CCTTCATCAG CATCAACCGC GGGGTGAGGG TGATGAAGGG CCACTCTGCC 
TTCCTGTCAG GAGATGACCT GAAGTTTGCC ATCCCTAAAG AGAAAGATGC CTGCAAAGTG 
GAAGTTGTGA TGAATGAGCC AATAACCCAG AGGGTTGGGA AACTCACTCC ACAGGTCTTT 
GACTGCCATT TCCTTCCCAA CGAAGTCAAG TATGTTCACA ATGGTTGTCC AATTCTTGAT 
GAAGACACAG TGAAGCTCAG ACTTTACAGA TTTACTGAAA GAGATACCTT CATAGAAACT 
TTTATCCTGT GGGTCTATCT CCTGGAACCA GACTGTAACA TCATCCATAT GAGTAACAAT 
GTGCTGGAGG TGCCTGAATT CAATGGCTTG TCCCAAGCGA TTGATAAAAA TCTGCTCAGA 
TTCGATTATG ATAGGATGGC TAGCCTGGAA TGTACCGTCA GCCTGGACAC TGCGAGAACT 
CGGCTGCCAG CCCATGGCCA GATGGTTCTC GGGGAGCCTC GACCAGAAGA ACCTCGTGGA 
GATCAGCCAC ACAGTTTTTT CCCAGAGTCC CGTATGATGT CTTTTAATGC CTTTCTGGAA 
CTGAGAGCAA AACTGAAGTG TCCAGGTGGG AGCTGTACCC CAGGATTAAA GAAAATAGGA 
AGCCTCAAAG TGAGCTGTGA GGAGTTCCTG CTGATGGGCC TTCGTTATCA GCATCTGGAT 
CCCCCTTCAC CCAACATTGA TTATATCTCC ATCCAACTGG ACCTCACAGA TACCAGGAGC 
AAAATTGTGT ACAAGTCAGA GAGTGCGTGG CTGCCTGTCT ATATCAGAGC TGGAATTCCG 
AATCAGATTC CAAAGGCTGC ATTCATGGCC GTGTTTATTC TGGAAGTGGA TCAGTTCATC 
CTGACCTCCT TGACTACATC AGTTCTGGAC TGTGAAGAAG ATGAGACCCC TAAACCCTTG 
CTGGTGTTCA ACATTACTAA AGCCCCGCTC CAGGGCTATG TGACTCACCT GTTGGATCAC 
ACCAGACCAA TCTCCTCATT CACCTGGAAA GATCTCAGTG ACATGCAGAT CGCCTATCAG 
CCACCAAACA GCAGCCATTC TGAGAGGAGA CATGATGAGG TAGAATTGGA GGTATACGAC 
TTCTTCTTTG AAAGGAGTGC ACCTATGACA GTCCACATCT CCATCAGAAC AGCAGATACA 
AATGCCCCCC GTGTATCCTG GAATACAGGT CTGAGTCTCC TTGAGGGGCA GTCTCGAGCC 
ATCACTTGGG AACAGTTTCA GGTTGTCGAC AATGACGACA TTGGTGCTGT CCGGCTAGTC 
ACCGTTGGTG GCCTGCAGCA TGGATGGCTG ACTTTAAGAG GGGGGAAAGG GTTTCTCTTC 
ACCGTGGCTG ACCTCCAGGC TGGAGTTGTT CGCTATCATC ATGATGACAG CGACTCCACC 
AAAGACTTCG TGGTCTTCCG GATATTTGAT GGCCATCACA GCATCCGTCA CAAATTCCCC 
ATCAACGTCT TGCCCAAAGA TGATAGTCCC CCGTTCCTCA TAACCAATGT TGTGATTGAA 
CTGGAGGAGG GGCAGACCAT CCTGATCCAG GGATCCATGC TGCGAGCTTC AGATGTGGAC 
GCCAGTGATG ACTACATCTT CTTCAATATC ACAAAGCCTC CACAGGCTGG GGAGATCATG 
AAGAAGCCAG GGCCAGGACT GATAGGCTAT CCTGTCCATG GCTTCCTTCA GAGGGATTTG 
TTTAATGGAA TCATTTATTA TCGTCATTTT GGTGGAGAAA TCTTTGAAGA TTCTTTTCAA 
TTTGTCCTGT GGGACAGCCA TGAACCTCCA AATCTTTCAG TGCCACAGGT GGCAACAATC 
CATATAACTC CAGTGGATGA CCAGCTTCCA AAAGAGGCTC CTGGAGTTTC TCGGCATTTG 
GTTGTCAAGG AAACTGAGGT GGCCTATATA ACTAAGAAAC AGCTACATTT TATAGATTCA 
GAATCATATG ACAGGGAGCT GGTCTACACA ATAACTACTC CTCCATTTTT CTCCTTCAGC 
CACAGACACT TGGATGCTGG GAAATTATTT ATGGTGGACA GCATACCAAA AGTAGTTAAG 
AATCCTACGG CCCTGGAGCT GAGGTCATTC ACTCAGCATG CTGTGAACTA TATGAAAGTG 
GCCTACATGC CCCCCATGCA AGACATTGGT CCCCATTGCA GAGATGTCCA GTTCACATTT 
TCTGTCAGTA ACCAACATGG CGGTACTTTG CATGGGATCT GCTTTAACAT TACAATCCTC 
CCAGTGGACA ATCAAGTTCC TGAGGCGTTC ACCAACCCTC TGAAAGTGAC TGAGGGAGGT 
CAAAGCATCA TCAGCACAGA GCACATTCTA ATTTCTGATG CAGATACCAA GCTGGACAAT 
ATTGACCTCT CCCTGCGGGA ATTGCCTCTG CACGGAAGGG TGGAGCTGAA TGGATTTCCT 
CTAAATTCAG GGGGCACATT TTCTTGGGGC GATCTCCATA CCTTAAAAGT TAGGTATCAA 
CATGATGGAA CTGAAGTTCT TCAGGATGAC CTACTCTTGG AGGTCACCGA TGGCACAAAT 
TCAGCAGAAT TTGTACTACA TGTTGAGGTA TTCCCTGTCA ACGATGAGCC ACCAGTCTTA 
AAGGCTGACC TCATGCCTGT CATGAATTGC TCAGAGGGAG GAGAGGTGGT CATCACCTCT 
GAATACATTT TTGCTACTGA TGTGGACAGC GATAACTTGA AGTTGATGTT TGTGATTGCT 
CGCGAACCTC AGCATGGGGT GGTGAGGAGA GCTGGAGTCA CAGTGGATCA GTTCTCTCAG 
AGAGATGTTA TCTCAGAGGC CGTGACATAC AAACACACAG GTGGCGAGAT TGGCCTGATG 
CCTTGTTTTG ACACCATTAC ATTGGTGGTT TCGGATGGAG AGGCTGGCCC TTTTGTGAAT 
GGCTGTTGCT ACAATGGACC CAATCCATCT GTTCCACTTC ATGCGTCCTT TCCAGTGTAT 
GATCTCAACA TCACGGTATA CCCAGTAGAC AACCAGCCAC CTTCCATTGC AATAGGTCCC 
GTGTTTGTTG TAGATGAGGG CTGCTCAACA GCCCTTACTG TTAACCATTT GTCCGCCACT 
GACCCTGACA CTGCAGCAGA TGACTTGGAA TTTGTTTTGG TTTCTCCTCC TCAGTTTGGC 
TACCTCGAAA ATATACTCCC TTCTGTGGGT TTTGAAAAAA GCAATATTGG CATAAGTATA 
GATTCATTTC AGTGGAAAGA CATGAACGCT TTTCACATTA ACTATGTGCA GTCCAGGCAT 
CTGAGGATAG AACCAACTGC CGACCAGTTC ACGGTGTACG TCACAGATGG GAAGCATCAC 
TCCTTGGAGA TACCATTTTC TATTATAATC AACCCCACAA ATGATGAAGC TCCTGACTTT 
GTAGTGCAGA ATATTACTGT GTGTGAGGGT CAGATGAAAG AGCTGGACTC TTCCATCATC 
AGCGCTGTGG ACCTGGACAT TCCCCAGGAT GCCCTGCTGT TCAGCATCAC TCAAAAGCCA 
CGCCATGGCC TCCTCATCGA TAGGGGGTTT AGCAAAGACT TCTCTGAGAA TAAGCAGCCA 
GCCAACCCTC ACCAGAAACA TGCACCTGTT CACAGCTTTT CCATGGAACT CCTCAAGACT 
GGAATGAGGT TGACGTACAT GCATGATGAC TCAGAGAGCC TTGCTGATGA TTTTACAATC 
CAATTGTCAG ATGGGAAACA TAAGATACTT AAAACCATTT CAGTAGAGGT CATCCCAGTT 
AATGATGAAA AACCAATGCT GAGCAAGAAG GCTGAAATTG CAATGAATAT GGGTGAAACT 
CGTATTATTT CCAGTGCTAT TCTTTCAGCC ATAGATGAAG ACTCACCCAG GGAGAAGATT 
TACTATGTAT TTGAAAGGCT TCCCCAAAAT GGGCAACTTC AGCTTAAGAT AGGGAGGGAC 
TGGGTTCCTC TCTCCCCTGG CATGAAATGC ACTCAGGAGG AAGTGGATCT GAACTTGCTG 
AGATACACAC ACACCGGGGC AATGGATTCC CAGAATCAAG ATAGCTTCAC CTTCTACCTT 
TGGGATGGCA ACAACAGGTC CCCTGCTCTT GACTGTCAAA TCACCATCAA GGACATGGAA 
AAAGGTGATA TTGTCATCCT TACAAAACCA CTCGTGGTGT CTAAAGGTGA TAGAGGTTTC 
TTAACAACCA CCACGCTCCT GGCTGTGGAC GGAACAGACA AGCCTGAGGA ACTGCTCTAT 
GTCATCACCT CCCCTCCGCG ATATGGCCAG ATCGAATATG TTCACTATCC TGGAGTTCCC 
ATTACAAACT TCAGCCAAAT GGATGTAGTG GGGCAGACAG TTTGCTATGT ACACAAGAGC 
AAGGTGACTG TCTCCAGTGA CAGATTCAGA TTCATCATCA GCAATGGACT GCGGACCGAG 
CACGGGGTGT TTGAGATCAC ACTGGAGACT GTGGACAGAG CCCTGCCTGT GGTAACCAGG 
AACAAGGGGT TGAGACTGGC CCAAGGGGCC GTGGGCCTGC TTTCCCCTGA CCTCCTTCAG 
CTGACCGACC CTGATACACC TGCGGAGAAC CTCACCTTCC TCTTGGTTCA GCTCCCCCAG 
CATGGCCAGC TCTACCTGTG GGGGACAGGG CTACTTCAAC ACAATTTCAC CCAGCAGGAT 
GTGGACAGCA AGAATGTGGC CTATCGGCAC TCAGGAGGGG ACTCCCAGAC TGACTGCTTT 
ACTTTCATGG CCACAGATGG GACAAACCAA GGCTTTATTG TGAATGGGAG AGTGTGGGAA 
GAACCTGTTT TATTCACCAT TCAGGTAGAC CAATTGGACA AAACAGCTCC TCGTATCACA 
CTCTTGCATT CCCCTTCTCA AGTGGGGCTC CTGAAAAATG GCTGCTACGG GATTTACATC 
ACTTCCCGCG TGTTGAAGGC ATCAGACCCT GACACTGAGG ACGATCAGAT CATCTTTAAA 
ATTCTACAAG GCCCAAAACA TGGACATCTG GAGAACACAA CAACAGGTGA ATTTATCCAT 
GAGAAATTTA GCCAAAAGGA CTTAAACAGT AAGACTATTC TTTACATCAT AAACCCATCT 
TTGGAAGTAA ATTCAGATAC CGTGGAATTT CAAATCATGG ACCCCACAGG GAACTCGGCC 
ACTCCTCAAA TTTTGGAACT GAAGTGGTCT CATATTGAAT GGTCACAGAC CGAATATGAA 
GTCTGTGAGA ATGTGGGTTT GTTGCCCTTG GAAATTATCA GAAGGGGATA TTCCATGGAC 
TCGGCCTTTG TGGGTATAAA GGTCAACCAA GTGTCAGCTG CAGTTGGAAA AGATTTCACC 
GTGATTCCAT CTAAACTGAT TCAGTTTGAC CCAGGAATGT CAACTAAGAT GTGGAATATA 
GCAATTACCT ATGACGGATT AGAGGAAGAT GATGAGGTCT TTGAAGTAAT TCTGAACTCC 
CCTGTGAATG CAGTTCTTGG CACAAAGACA AAAGCTGCAG TGAAAATTTT GGACTCAAAA 
GGAGGACAAT GCCATCCTTC ATATTCCTCC AACCAAAGCA AGCACAGCAC ATGGGAGAAG 
GGCATTTGGC ATCTGCTGCC CCCAGGGTCT TCCTCATCCA CCACTTCTGG TTCCTTTCAT 
CTGGAAAGAA GACCTCTTCC ATCTTCCATG CAGCTAGCAG TCATCAGGGG AGACACCCTG 
CGGGGCTTTG ATTCTACAGA TCTTTCTCAA AGGAAGCTTA GGACCCGTGG GAATGGCAAA 
ACAGTTCGTC CATCCTCTGT TTATAGAAAT GGAACAGACA TCATCTATAA TTATCATGGG 
ATAGTTTCCT TGAAACTGGA GGATGACAGT TTCCCAACTC ACAAAAGGAA GGCCAAAGTA 
TCCATCATTA GTCAGCCACA AAAGACAATC AAAGTGGCAG AACTGCCTCA AGCAGATAAG 
GTGGAATCCA CAACTGACTC ACACTTCCCC AGACAGGACC AGTTGCCCTC ATTTCCAAAG 
AACTGCACTC TGGAATTAAA GGGACTCTTC CATTTTGAAG AAGGCATCCA GAAGCTGTAT 
CAGTGCAATG GGATCGCCTG GAAAGCCTGG AGTCCCCAAA CCAAGGATGT GGAAGACAAA 
TCCTGTCCAG CCGGGTGGCA CCAGCACTCA GGCTACTGTC ACATCTTGAT CACAGAGCAG 
AAAGGCACCT GGAATGCGGC TGCCCAAGCT TGCAGGGAAC AATACCTGGG CAACCTTGTA 
ACTGTATTCT CCAGGCAGCA CATGCGGTGG CTCTGGGACA TTGGTGGGAG AAAGTCCTTT 
TGGATAGGTT TGAACGACCA AGTGCATGCT GGCCACTGGG AGTGGATCGG TGGTGAACCT 
GTTGCCTTCA CCAATGGGAG AAGAGGGCCC TCTCAACGCT CCAAGCTTGG AAAGAGCTGT 
GTTTTGGTTC AAAGACAAGG GAAATGGCAA ACAAAAGACT GTAGGAGAGC CAAACCTCAT 
AATTATGTGT GTTCCAGAAA ACTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016869808.1
CDS	1267..7833
Translation	MNSLSWGAANAVLLLLLLAWASPTFISINRGVRVMKGHSAFLSGDDLKFAIPKEKDACKVEVVMNEPITQRVGKLTPQVFDCHFLPNEVKYVHNGCPILDEDTVKLRLYRFTERDTFIETFILWVYLLEPDCNIIHMSNNVLEVPEFNGLSQAIDKNLLRFDYDRMASLECTVSLDTARTRLPAHGQMVLGEPRPEEPRGDQPHSFFPESRMMSFNAFLELRAKLKCPGGSCTPGLKKIGSLKVSCEEFLLMGLRYQHLDPPSPNIDYISIQLDLTDTRSKIVYKSESAWLPVYIRAGIPNQIPKAAFMAVFILEVDQFILTSLTTSVLDCEEDETPKPLLVFNITKAPLQGYVTHLLDHTRPISSFTWKDLSDMQIAYQPPNSSHSERRHDEVELEVYDFFFERSAPMTVHISIRTADTNAPRVSWNTGLSLLEGQSRAITWEQFQVVDNDDIGAVRLVTVGGLQHGWLTLRGGKGFLFTVADLQAGVVRYHHDDSDSTKDFVVFRIFDGHHSIRHKFPINVLPKDDSPPFLITNVVIELEEGQTILIQGSMLRASDVDASDDYIFFNITKPPQAGEIMKKPGPGLIGYPVHGFLQRDLFNGIIYYRHFGGEIFEDSFQFVLWDSHEPPNLSVPQVATIHITPVDDQLPKEAPGVSRHLVVKETEVAYITKKQLHFIDSESYDRELVYTITTPPFFSFSHRHLDAGKLFMVDSIPKVVKNPTALELRSFTQHAVNYMKVAYMPPMQDIGPHCRDVQFTFSVSNQHGGTLHGICFNITILPVDNQVPEAFTNPLKVTEGGQSIISTEHILISDADTKLDNIDLSLRELPLHGRVELNGFPLNSGGTFSWGDLHTLKVRYQHDGTEVLQDDLLLEVTDGTNSAEFVLHVEVFPVNDEPPVLKADLMPVMNCSEGGEVVITSEYIFATDVDSDNLKLMFVIAREPQHGVVRRAGVTVDQFSQRDVISEAVTYKHTGGEIGLMPCFDTITLVVSDGEAGPFVNGCCYNGPNPSVPLHASFPVYDLNITVYPVDNQPPSIAIGPVFVVDEGCSTALTVNHLSATDPDTAADDLEFVLVSPPQFGYLENILPSVGFEKSNIGISIDSFQWKDMNAFHINYVQSRHLRIEPTADQFTVYVTDGKHHSLEIPFSIIINPTNDEAPDFVVQNITVCEGQMKELDSSIISAVDLDIPQDALLFSITQKPRHGLLIDRGFSKDFSENKQPANPHQKHAPVHSFSMELLKTGMRLTYMHDDSESLADDFTIQLSDGKHKILKTISVEVIPVNDEKPMLSKKAEIAMNMGETRIISSAILSAIDEDSPREKIYYVFERLPQNGQLQLKIGRDWVPLSPGMKCTQEEVDLNLLRYTHTGAMDSQNQDSFTFYLWDGNNRSPALDCQITIKDMEKGDIVILTKPLVVSKGDRGFLTTTTLLAVDGTDKPEELLYVITSPPRYGQIEYVHYPGVPITNFSQMDVVGQTVCYVHKSKVTVSSDRFRFIISNGLRTEHGVFEITLETVDRALPVVTRNKGLRLAQGAVGLLSPDLLQLTDPDTPAENLTFLLVQLPQHGQLYLWGTGLLQHNFTQQDVDSKNVAYRHSGGDSQTDCFTFMATDGTNQGFIVNGRVWEEPVLFTIQVDQLDKTAPRITLLHSPSQVGLLKNGCYGIYITSRVLKASDPDTEDDQIIFKILQGPKHGHLENTTTGEFIHEKFSQKDLNSKTILYIINPSLEVNSDTVEFQIMDPTGNSATPQILELKWSHIEWSQTEYEVCENVGLLPLEIIRRGYSMDSAFVGIKVNQVSAAVGKDFTVIPSKLIQFDPGMSTKMWNIAITYDGLEEDDEVFEVILNSPVNAVLGTKTKAAVKILDSKGGQCHPSYSSNQSKHSTWEKGIWHLLPPGSSSSTTSGSFHLERRPLPSSMQLAVIRGDTLRGFDSTDLSQRKLRTRGNGKTVRPSSVYRNGTDIIYNYHGIVSLKLEDDSFPTHKRKAKVSIISQPQKTIKVAELPQADKVESTTDSHFPRQDQLPSFPKNCTLELKGLFHFEEGIQKLYQCNGIAWKAWSPQTKDVEDKSCPAGWHQHSGYCHILITEQKGTWNAAAQACREQYLGNLVTVFSRQHMRWLWDIGGRKSFWIGLNDQVHAGHWEWIGGEPVAFTNGRRGPSQRSKLGKSCVLVQRQGKWQTKDCRRAKPHNYVCSRKL

Target ORF information:

RefSeq Version	XM_017014319.1
Organism	Homo sapiens(human)
Definition	Homo sapiens FRAS1 related extracellular matrix 1 (FREM1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017014319.1

ORF Insert Sequence:

ATGAACTCTC TGAGTTGGGG GGCTGCGAAT GCCGTGCTGC TGCTGCTCCT CCTGGCCTGG 
GCCAGCCCCA CCTTCATCAG CATCAACCGC GGGGTGAGGG TGATGAAGGG CCACTCTGCC 
TTCCTGTCAG GAGATGACCT GAAGTTTGCC ATCCCTAAAG AGAAAGATGC CTGCAAAGTG 
GAAGTTGTGA TGAATGAGCC AATAACCCAG AGGGTTGGGA AACTCACTCC ACAGGTCTTT 
GACTGCCATT TCCTTCCCAA CGAAGTCAAG TATGTTCACA ATGGTTGTCC AATTCTTGAT 
GAAGACACAG TGAAGCTCAG ACTTTACAGA TTTACTGAAA GAGATACCTT CATAGAAACT 
TTTATCCTGT GGGTCTATCT CCTGGAACCA GACTGTAACA TCATCCATAT GAGTAACAAT 
GTGCTGGAGG TGCCTGAATT CAATGGCTTG TCCCAAGCGA TTGATAAAAA TCTGCTCAGA 
TTCGATTATG ATAGGATGGC TAGCCTGGAA TGTACCGTCA GCCTGGACAC TGCGAGAACT 
CGGCTGCCAG CCCATGGCCA GATGGTTCTC GGGGAGCCTC GACCAGAAGA ACCTCGTGGA 
GATCAGCCAC ACAGTTTTTT CCCAGAGTCC CGTATGATGT CTTTTAATGC CTTTCTGGAA 
CTGAGAGCAA AACTGAAGTG TCCAGGTGGG AGCTGTACCC CAGGATTAAA GAAAATAGGA 
AGCCTCAAAG TGAGCTGTGA GGAGTTCCTG CTGATGGGCC TTCGTTATCA GCATCTGGAT 
CCCCCTTCAC CCAACATTGA TTATATCTCC ATCCAACTGG ACCTCACAGA TACCAGGAGC 
AAAATTGTGT ACAAGTCAGA GAGTGCGTGG CTGCCTGTCT ATATCAGAGC TGGAATTCCG 
AATCAGATTC CAAAGGCTGC ATTCATGGCC GTGTTTATTC TGGAAGTGGA TCAGTTCATC 
CTGACCTCCT TGACTACATC AGTTCTGGAC TGTGAAGAAG ATGAGACCCC TAAACCCTTG 
CTGGTGTTCA ACATTACTAA AGCCCCGCTC CAGGGCTATG TGACTCACCT GTTGGATCAC 
ACCAGACCAA TCTCCTCATT CACCTGGAAA GATCTCAGTG ACATGCAGAT CGCCTATCAG 
CCACCAAACA GCAGCCATTC TGAGAGGAGA CATGATGAGG TAGAATTGGA GGTATACGAC 
TTCTTCTTTG AAAGGAGTGC ACCTATGACA GTCCACATCT CCATCAGAAC AGCAGATACA 
AATGCCCCCC GTGTATCCTG GAATACAGGT CTGAGTCTCC TTGAGGGGCA GTCTCGAGCC 
ATCACTTGGG AACAGTTTCA GGTTGTCGAC AATGACGACA TTGGTGCTGT CCGGCTAGTC 
ACCGTTGGTG GCCTGCAGCA TGGATGGCTG ACTTTAAGAG GGGGGAAAGG GTTTCTCTTC 
ACCGTGGCTG ACCTCCAGGC TGGAGTTGTT CGCTATCATC ATGATGACAG CGACTCCACC 
AAAGACTTCG TGGTCTTCCG GATATTTGAT GGCCATCACA GCATCCGTCA CAAATTCCCC 
ATCAACGTCT TGCCCAAAGA TGATAGTCCC CCGTTCCTCA TAACCAATGT TGTGATTGAA 
CTGGAGGAGG GGCAGACCAT CCTGATCCAG GGATCCATGC TGCGAGCTTC AGATGTGGAC 
GCCAGTGATG ACTACATCTT CTTCAATATC ACAAAGCCTC CACAGGCTGG GGAGATCATG 
AAGAAGCCAG GGCCAGGACT GATAGGCTAT CCTGTCCATG GCTTCCTTCA GAGGGATTTG 
TTTAATGGAA TCATTTATTA TCGTCATTTT GGTGGAGAAA TCTTTGAAGA TTCTTTTCAA 
TTTGTCCTGT GGGACAGCCA TGAACCTCCA AATCTTTCAG TGCCACAGGT GGCAACAATC 
CATATAACTC CAGTGGATGA CCAGCTTCCA AAAGAGGCTC CTGGAGTTTC TCGGCATTTG 
GTTGTCAAGG AAACTGAGGT GGCCTATATA ACTAAGAAAC AGCTACATTT TATAGATTCA 
GAATCATATG ACAGGGAGCT GGTCTACACA ATAACTACTC CTCCATTTTT CTCCTTCAGC 
CACAGACACT TGGATGCTGG GAAATTATTT ATGGTGGACA GCATACCAAA AGTAGTTAAG 
AATCCTACGG CCCTGGAGCT GAGGTCATTC ACTCAGCATG CTGTGAACTA TATGAAAGTG 
GCCTACATGC CCCCCATGCA AGACATTGGT CCCCATTGCA GAGATGTCCA GTTCACATTT 
TCTGTCAGTA ACCAACATGG CGGTACTTTG CATGGGATCT GCTTTAACAT TACAATCCTC 
CCAGTGGACA ATCAAGTTCC TGAGGCGTTC ACCAACCCTC TGAAAGTGAC TGAGGGAGGT 
CAAAGCATCA TCAGCACAGA GCACATTCTA ATTTCTGATG CAGATACCAA GCTGGACAAT 
ATTGACCTCT CCCTGCGGGA ATTGCCTCTG CACGGAAGGG TGGAGCTGAA TGGATTTCCT 
CTAAATTCAG GGGGCACATT TTCTTGGGGC GATCTCCATA CCTTAAAAGT TAGGTATCAA 
CATGATGGAA CTGAAGTTCT TCAGGATGAC CTACTCTTGG AGGTCACCGA TGGCACAAAT 
TCAGCAGAAT TTGTACTACA TGTTGAGGTA TTCCCTGTCA ACGATGAGCC ACCAGTCTTA 
AAGGCTGACC TCATGCCTGT CATGAATTGC TCAGAGGGAG GAGAGGTGGT CATCACCTCT 
GAATACATTT TTGCTACTGA TGTGGACAGC GATAACTTGA AGTTGATGTT TGTGATTGCT 
CGCGAACCTC AGCATGGGGT GGTGAGGAGA GCTGGAGTCA CAGTGGATCA GTTCTCTCAG 
AGAGATGTTA TCTCAGAGGC CGTGACATAC AAACACACAG GTGGCGAGAT TGGCCTGATG 
CCTTGTTTTG ACACCATTAC ATTGGTGGTT TCGGATGGAG AGGCTGGCCC TTTTGTGAAT 
GGCTGTTGCT ACAATGGACC CAATCCATCT GTTCCACTTC ATGCGTCCTT TCCAGTGTAT 
GATCTCAACA TCACGGTATA CCCAGTAGAC AACCAGCCAC CTTCCATTGC AATAGGTCCC 
GTGTTTGTTG TAGATGAGGG CTGCTCAACA GCCCTTACTG TTAACCATTT GTCCGCCACT 
GACCCTGACA CTGCAGCAGA TGACTTGGAA TTTGTTTTGG TTTCTCCTCC TCAGTTTGGC 
TACCTCGAAA ATATACTCCC TTCTGTGGGT TTTGAAAAAA GCAATATTGG CATAAGTATA 
GATTCATTTC AGTGGAAAGA CATGAACGCT TTTCACATTA ACTATGTGCA GTCCAGGCAT 
CTGAGGATAG AACCAACTGC CGACCAGTTC ACGGTGTACG TCACAGATGG GAAGCATCAC 
TCCTTGGAGA TACCATTTTC TATTATAATC AACCCCACAA ATGATGAAGC TCCTGACTTT 
GTAGTGCAGA ATATTACTGT GTGTGAGGGT CAGATGAAAG AGCTGGACTC TTCCATCATC 
AGCGCTGTGG ACCTGGACAT TCCCCAGGAT GCCCTGCTGT TCAGCATCAC TCAAAAGCCA 
CGCCATGGCC TCCTCATCGA TAGGGGGTTT AGCAAAGACT TCTCTGAGAA TAAGCAGCCA 
GCCAACCCTC ACCAGAAACA TGCACCTGTT CACAGCTTTT CCATGGAACT CCTCAAGACT 
GGAATGAGGT TGACGTACAT GCATGATGAC TCAGAGAGCC TTGCTGATGA TTTTACAATC 
CAATTGTCAG ATGGGAAACA TAAGATACTT AAAACCATTT CAGTAGAGGT CATCCCAGTT 
AATGATGAAA AACCAATGCT GAGCAAGAAG GCTGAAATTG CAATGAATAT GGGTGAAACT 
CGTATTATTT CCAGTGCTAT TCTTTCAGCC ATAGATGAAG ACTCACCCAG GGAGAAGATT 
TACTATGTAT TTGAAAGGCT TCCCCAAAAT GGGCAACTTC AGCTTAAGAT AGGGAGGGAC 
TGGGTTCCTC TCTCCCCTGG CATGAAATGC ACTCAGGAGG AAGTGGATCT GAACTTGCTG 
AGATACACAC ACACCGGGGC AATGGATTCC CAGAATCAAG ATAGCTTCAC CTTCTACCTT 
TGGGATGGCA ACAACAGGTC CCCTGCTCTT GACTGTCAAA TCACCATCAA GGACATGGAA 
AAAGGTGATA TTGTCATCCT TACAAAACCA CTCGTGGTGT CTAAAGGTGA TAGAGGTTTC 
TTAACAACCA CCACGCTCCT GGCTGTGGAC GGAACAGACA AGCCTGAGGA ACTGCTCTAT 
GTCATCACCT CCCCTCCGCG ATATGGCCAG ATCGAATATG TTCACTATCC TGGAGTTCCC 
ATTACAAACT TCAGCCAAAT GGATGTAGTG GGGCAGACAG TTTGCTATGT ACACAAGAGC 
AAGGTGACTG TCTCCAGTGA CAGATTCAGA TTCATCATCA GCAATGGACT GCGGACCGAG 
CACGGGGTGT TTGAGATCAC ACTGGAGACT GTGGACAGAG CCCTGCCTGT GGTAACCAGG 
AACAAGGGGT TGAGACTGGC CCAAGGGGCC GTGGGCCTGC TTTCCCCTGA CCTCCTTCAG 
CTGACCGACC CTGATACACC TGCGGAGAAC CTCACCTTCC TCTTGGTTCA GCTCCCCCAG 
CATGGCCAGC TCTACCTGTG GGGGACAGGG CTACTTCAAC ACAATTTCAC CCAGCAGGAT 
GTGGACAGCA AGAATGTGGC CTATCGGCAC TCAGGAGGGG ACTCCCAGAC TGACTGCTTT 
ACTTTCATGG CCACAGATGG GACAAACCAA GGCTTTATTG TGAATGGGAG AGTGTGGGAA 
GAACCTGTTT TATTCACCAT TCAGGTAGAC CAATTGGACA AAACAGCTCC TCGTATCACA 
CTCTTGCATT CCCCTTCTCA AGTGGGGCTC CTGAAAAATG GCTGCTACGG GATTTACATC 
ACTTCCCGCG TGTTGAAGGC ATCAGACCCT GACACTGAGG ACGATCAGAT CATCTTTAAA 
ATTCTACAAG GCCCAAAACA TGGACATCTG GAGAACACAA CAACAGGTGA ATTTATCCAT 
GAGAAATTTA GCCAAAAGGA CTTAAACAGT AAGACTATTC TTTACATCAT AAACCCATCT 
TTGGAAGTAA ATTCAGATAC CGTGGAATTT CAAATCATGG ACCCCACAGG GAACTCGGCC 
ACTCCTCAAA TTTTGGAACT GAAGTGGTCT CATATTGAAT GGTCACAGAC CGAATATGAA 
GTCTGTGAGA ATGTGGGTTT GTTGCCCTTG GAAATTATCA GAAGGGGATA TTCCATGGAC 
TCGGCCTTTG TGGGTATAAA GGTCAACCAA GTGTCAGCTG CAGTTGGAAA AGATTTCACC 
GTGATTCCAT CTAAACTGAT TCAGTTTGAC CCAGGAATGT CAACTAAGAT GTGGAATATA 
GCAATTACCT ATGACGGATT AGAGGAAGAT GATGAGGTCT TTGAAGTAAT TCTGAACTCC 
CCTGTGAATG CAGTTCTTGG CACAAAGACA AAAGCTGCAG TGAAAATTTT GGACTCAAAA 
GGAGGACAAT GCCATCCTTC ATATTCCTCC AACCAAAGCA AGCACAGCAC ATGGGAGAAG 
GGCATTTGGC ATCTGCTGCC CCCAGGGTCT TCCTCATCCA CCACTTCTGG TTCCTTTCAT 
CTGGAAAGAA GACCTCTTCC ATCTTCCATG CAGCTAGCAG TCATCAGGGG AGACACCCTG 
CGGGGCTTTG ATTCTACAGA TCTTTCTCAA AGGAAGCTTA GGACCCGTGG GAATGGCAAA 
ACAGTTCGTC CATCCTCTGT TTATAGAAAT GGAACAGACA TCATCTATAA TTATCATGGG 
ATAGTTTCCT TGAAACTGGA GGATGACAGT TTCCCAACTC ACAAAAGGAA GGCCAAAGTA 
TCCATCATTA GTCAGCCACA AAAGACAATC AAAGTGGCAG AACTGCCTCA AGCAGATAAG 
GTGGAATCCA CAACTGACTC ACACTTCCCC AGACAGGACC AGTTGCCCTC ATTTCCAAAG 
AACTGCACTC TGGAATTAAA GGGACTCTTC CATTTTGAAG AAGGCATCCA GAAGCTGTAT 
CAGTGCAATG GGATCGCCTG GAAAGCCTGG AGTCCCCAAA CCAAGGATGT GGAAGACAAA 
TCCTGTCCAG CCGGGTGGCA CCAGCACTCA GGCTACTGTC ACATCTTGAT CACAGAGCAG 
AAAGGCACCT GGAATGCGGC TGCCCAAGCT TGCAGGGAAC AATACCTGGG CAACCTTGTA 
ACTGTATTCT CCAGGCAGCA CATGCGGTGG CTCTGGGACA TTGGTGGGAG AAAGTCCTTT 
TGGATAGGTT TGAACGACCA AGTGCATGCT GGCCACTGGG AGTGGATCGG TGGTGAACCT 
GTTGCCTTCA CCAATGGGAG AAGAGGGCCC TCTCAACGCT CCAAGCTTGG AAAGAGCTGT 
GTTTTGGTTC AAAGACAAGG GAAATGGCAA ACAAAAGACT GTAGGAGAGC CAAACCTCAT 
AATTATGTGT GTTCCAGAAA ACTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FREM1 ( XM_017014324.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016869813.1 Homo sapiens FRAS1 related extracellular matrix 1 (FREM1), transcript variant X10, mRNA.

Price & Availability↑

CloneID	OHu98460
Clone ID Related Accession (Same CDS sequence)	XM_017014324.1 , XM_017014324.2
Accession Version	XM_017014324.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6417bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	FRAS1-related extracellular matrix protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACTCTC TGAGTTGGGG GGCTGCGAAT GCCGTGCTGC TGCTGCTCCT CCTGGCCTGG 
GCCAGCCCCA CCTTCATCAG CATCAACCGC GGGGTGAGGG TGATGAAGGG CCACTCTGCC 
TTCCTGTCAG GAGATGACCT GAAGTTTGCC ATCCCTAAAG AGAAAGATGC CTGCAAAGTG 
GAAGTTGTGA TGAATGAGCC AATAACCCAG AGGGTTGGGA AACTCACTCC ACAGGTCTTT 
GACTGCCATT TCCTTCCCAA CGAAGTCAAG TATGTTCACA ATGGTTGTCC AATTCTTGAT 
GAAGACACAG TGAAGCTCAG ACTTTACAGA TTTACTGAAA GAGATACCTT CATAGAAACT 
TTTATCCTGT GGGTCTATCT CCTGGAACCA GACTGTAACA TCATCCATAT GAGTAACAAT 
GTGCTGGAGG TGCCTGAATT CAATGGCTTG TCCCAAGCGA TTGATAAAAA TCTGCTCAGA 
TTCGATTATG ATAGGATGGC TAGCCTGGAA TGTACCGTCA GCCTGGACAC TGCGAGAACT 
CGGCTGCCAG CCCATGGCCA GATGGTTCTC GGGGAGCCTC GACCAGAAGA ACCTCGTGGA 
GATCAGCCAC ACAGTTTTTT CCCAGAGTCC CGTATGATGT CTTTTAATGC CTTTCTGGAA 
CTGAGAGCAA AACTGAAGTG TCCAGGTGGG AGCTGTACCC CAGGATTAAA GAAAATAGGA 
AGCCTCAAAG TGAGCTGTGA GGAGTTCCTG CTGATGGGCC TTCGTTATCA GCATCTGGAT 
CCCCCTTCAC CCAACATTGA TTATATCTCC ATCCAACTGG ACCTCACAGA TACCAGGAGC 
AAAATTGTGT ACAAGTCAGA GAGTGCGTGG CTGCCTGTCT ATATCAGAGC TGGAATTCCG 
AATCAGATTC CAAAGGCTGC ATTCATGGCC GTGTTTATTC TGGAAGTGGA TCAGTTCATC 
CTGACCTCCT TGACTACATC AGTTCTGGAC TGTGAAGAAG ATGAGACCCC TAAACCCTTG 
CTGGTGTTCA ACATTACTAA AGCCCCGCTC CAGGGCTATG TGACTCACCT GTTGGATCAC 
ACCAGACCAA TCTCCTCATT CACCTGGAAA GATCTCAGTG ACATGCAGAT CGCCTATCAG 
CCACCAAACA GCAGCCATTC TGAGAGGAGA CATGATGAGG TAGAATTGGA GGTATACGAC 
TTCTTCTTTG AAAGGAGTGC ACCTATGACA GTCCACATCT CCATCAGAAC AGCAGATACA 
AATGCCCCCC GTGTATCCTG GAATACAGGT CTGAGTCTCC TTGAGGGGCA GTCTCGAGCC 
ATCACTTGGG AACAGTTTCA GGTTGTCGAC AATGACGACA TTGGTGCTGT CCGGCTAGTC 
ACCGTTGGTG GCCTGCAGCA TGGATGGCTG ACTTTAAGAG GGGGGAAAGG GTTTCTCTTC 
ACCGTGGCTG ACCTCCAGGC TGGAGTTGTT CGCTATCATC ATGATGACAG CGACTCCACC 
AAAGACTTCG TGGTCTTCCG GATATTTGAT GGCCATCACA GCATCCGTCA CAAATTCCCC 
ATCAACGTCT TGCCCAAAGA TGATAGTCCC CCGTTCCTCA TAACCAATGT TGTGATTGAA 
CTGGAGGAGG GGCAGACCAT CCTGATCCAG GGATCCATGC TGCGAGCTTC AGATGTGGAC 
GCCAGTGATG ACTACATCTT CTTCAATATC ACAAAGCCTC CACAGGCTGG GGAGATCATG 
AAGAAGCCAG GGCCAGGACT GATAGGCTAT CCTGTCCATG GCTTCCTTCA GAGGGATTTG 
TTTAATGGAA TCATTTATTA TCGTCATTTT GGTGGAGAAA TCTTTGAAGA TTCTTTTCAA 
TTTGTCCTGT GGGACAGCCA TGAACCTCCA AATCTTTCAG TGCCACAGGT GGCAACAATC 
CATATAACTC CAGTGGATGA CCAGCTTCCA AAAGAGGCTC CTGGAGTTTC TCGGCATTTG 
GTTGTCAAGG AAACTGAGGT GGCCTATATA ACTAAGAAAC AGCTACATTT TATAGATTCA 
GAATCATATG ACAGGGAGCT GGTCTACACA ATAACTACTC CTCCATTTTT CTCCTTCAGC 
CACAGACACT TGGATGCTGG GAAATTATTT ATGGTGGACA GCATACCAAA AGTAGTTAAG 
AATCCTACGG CCCTGGAGCT GAGGTCATTC ACTCAGCATG CTGTGAACTA TATGAAAGTG 
GCCTACATGC CCCCCATGCA AGACATTGGT CCCCATTGCA GAGATGTCCA GTTCACATTT 
TCTGTCAGTA ACCAACATGG CGGTACTTTG CATGGGATCT GCTTTAACAT TACAATCCTC 
CCAGTGGACA ATCAAGTTCC TGAGGCGTTC ACCAACCCTC TGAAAGTGAC TGAGGGAGGT 
CAAAGCATCA TCAGCACAGA GCACATTCTA ATTTCTGATG CAGATACCAA GCTGGACAAT 
ATTGACCTCT CCCTGCGGGA ATTGCCTCTG CACGGAAGGG TGGAGCTGAA TGGATTTCCT 
CTAAATTCAG GGGGCACATT TTCTTGGGGC GATCTCCATA CCTTAAAAGT TAGGTATCAA 
CATGATGGAA CTGAAGTTCT TCAGGATGAC CTACTCTTGG AGGTCACCGA TGGCACAAAT 
TCAGCAGAAT TTGTACTACA TGTTGAGGTA TTCCCTGTCA ACGATGAGCC ACCAGTCTTA 
AAGGCTGACC TCATGCCTGT CATGAATTGC TCAGAGGGAG GAGAGGTGGT CATCACCTCT 
GAATACATTT TTGCTACTGA TGTGGACAGC GATAACTTGA AGTTGATGTT TGTGATTGCT 
CGCGAACCTC AGCATGGGGT GGTGAGGAGA GCTGGAGTCA CAGTGGATCA GTTCTCTCAG 
AGAGATGTTA TCTCAGAGGC CGTGACATAC AAACACACAG GTGGCGAGAT TGGCCTGATG 
CCTTGTTTTG ACACCATTAC ATTGGTGGTT TCGGATGGAG AGGCTGGCCC TTTTGTGAAT 
GGCTGTTGCT ACAATGGACC CAATCCATCT GTTCCACTTC ATGCGTCCTT TCCAGTGTAT 
GATCTCAACA TCACGGTATA CCCAGTAGAC AACCAGCCAC CTTCCATTGC AATAGGTCCC 
GTGTTTGTTG TAGATGAGGG CTGCTCAACA GCCCTTACTG TTAACCATTT GTCCGCCACT 
GACCCTGACA CTGCAGCAGA TGACTTGGAA TTTGTTTTGG TTTCTCCTCC TCAGTTTGGC 
TACCTCGAAA ATATACTCCC TTCTGTGGGT TTTGAAAAAA GCAATATTGG CATAAGTATA 
GATTCATTTC AGTGGAAAGA CATGAACGCT TTTCACATTA ACTATGTGCA GTCCAGGCAT 
CTGAGGATAG AACCAACTGC CGACCAGTTC ACGGTGTACG TCACAGATGG GAAGCATCAC 
TCCTTGGAGA TACCATTTTC TATTATAATC AACCCCACAA ATGATGAAGC TCCTGACTTT 
GTAGTGCAGA ATATTACTGT GTGTGAGGGT CAGATGAAAG AGCTGGACTC TTCCATCATC 
AGCGCTGTGG ACCTGGACAT TCCCCAGGAT GCCCTGCTGT TCAGCATCAC TCAAAAGCCA 
CGCCATGGCC TCCTCATCGA TAGGGGGTTT AGCAAAGACT TCTCTGAGAA TAAGCAGCCA 
GCCAACCCTC ACCAGAAACA TGCACCTGTT CACAGCTTTT CCATGGAACT CCTCAAGACT 
GGAATGAGGT TGACGTACAT GCATGATGAC TCAGAGAGCC TTGCTGATGA TTTTACAATC 
CAATTGTCAG ATGGGAAACA TAAGATACTT AAAACCATTT CAGTAGAGGT CATCCCAGTT 
AATGATGAAA AACCAATGCT GAGCAAGAAG GCTGAAATTG CAATGAATAT GGGTGAAACT 
CGTATTATTT CCAGTGCTAT TCTTTCAGCC ATAGATGAAG ACTCACCCAG GGAGAAGATT 
TACTATGTAT TTGAAAGGCT TCCCCAAAAT GGGCAACTTC AGCTTAAGAT AGGGAGGGAC 
TGGGTTCCTC TCTCCCCTGG CATGAAATGC ACTCAGGAGG AAGTGGATCT GAACTTGCTG 
AGATACACAC ACACCGGGGC AATGGATTCC CAGAATCAAG ATAGCTTCAC CTTCTACCTT 
TGGGATGGCA ACAACAGGTC CCCTGCTCTT GACTGTCAAA TCACCATCAA GGACATGGAA 
AAAGGTGATA TTGTCATCCT TACAAAACCA CTCGTGGTGT CTAAAGGTGA TAGAGGTTTC 
TTAACAACCA CCACGCTCCT GGCTGTGGAC GGAACAGACA AGCCTGAGGA ACTGCTCTAT 
GTCATCACCT CCCCTCCGCG ATATGGCCAG ATCGAATATG TTCACTATCC TGGAGTTCCC 
ATTACAAACT TCAGCCAAAT GGATGTAGTG GGGCAGACAG TTTGCTATGT ACACAAGAGC 
AAGGTGACTG TCTCCAGTGA CAGATTCAGA TTCATCATCA GCAATGGACT GCGGACCGAG 
CACGGGGTGT TTGAGATCAC ACTGGAGACT GTGGACAGAG CCCTGCCTGT GGTAACCAGG 
AACAAGGGGT TGAGACTGGC CCAAGGGGCC GTGGGCCTGC TTTCCCCTGA CCTCCTTCAG 
CTGACCGACC CTGATACACC TGCGGAGAAC CTCACCTTCC TCTTGGTTCA GCTCCCCCAG 
CATGGCCAGC TCTACCTGTG GGGGACAGGG CTACTTCAAC ACAATTTCAC CCAGCAGGAT 
GTGGACAGCA AGAATGTGGC CTATCGGCAC TCAGGAGGGG ACTCCCAGAC TGACTGCTTT 
ACTTTCATGG CCACAGATGG GACAAACCAA GGCTTTATTG TGAATGGGAG AGTGTGGGAA 
GAACCTGTTT TATTCACCAT TCAGGTAGAC CAATTGGACA AAACAGCTCC TCGTATCACA 
CTCTTGCATT CCCCTTCTCA AGTGGGGCTC CTGAAAAATG GCTGCTACGG GATTTACATC 
ACTTCCCGCG TGTTGAAGGC ATCAGACCCT GACACTGAGG ACGATCAGAT CATCTTTAAA 
ATTCTACAAG GCCCAAAACA TGGACATCTG GAGAACACAA CAACAGGTGA ATTTATCCAT 
GAGAAATTTA GCCAAAAGGA CTTAAACAGT AAGACTATTC TTTACATCAT AAACCCATCT 
TTGGAAGTAA ATTCAGATAC CGTGGAATTT CAAATCATGG ACCCCACAGG GAACTCGGCC 
ACTCCTCAAA TTTTGGAACT GAAGTGGTCT CATATTGAAT GGTCACAGAC CGAATATGAA 
GTCTGTGAGA ATGTGGGTTT GTTGCCCTTG GAAATTATCA GAAGGGGATA TTCCATGGAC 
TCGGCCTTTG TGGGTATAAA GGTCAACCAA GTGTCAGCTG CAGTTGGAAA AGATTTCACC 
GTGATTCCAT CTAAACTGAT TCAGTTTGAC CCAGGACAAT GCCATCCTTC ATATTCCTCC 
AACCAAAGCA AGCACAGCAC ATGGGAGAAG GGCATTTGGC ATCTGCTGCC CCCAGGGTCT 
TCCTCATCCA CCACTTCTGG TTCCTTTCAT CTGGAAAGAA GACCTCTTCC ATCTTCCATG 
CAGCTAGCAG TCATCAGGGG AGACACCCTG CGGGGCTTTG ATTCTACAGA TCTTTCTCAA 
AGGAAGCTTA GGACCCGTGG GAATGGCAAA ACAGTTCGTC CATCCTCTGT TTATAGAAAT 
GGAACAGACA TCATCTATAA TTATCATGGG ATAGTTTCCT TGAAACTGGA GGATGACAGT 
TTCCCAACTC ACAAAAGGAA GGCCAAAGTA TCCATCATTA GTCAGCCACA AAAGACAATC 
AAAGTGGCAG AACTGCCTCA AGCAGATAAG GTGGAATCCA CAACTGACTC ACACTTCCCC 
AGACAGGACC AGTTGCCCTC ATTTCCAAAG AACTGCACTC TGGAATTAAA GGGACTCTTC 
CATTTTGAAG AAGGCATCCA GAAGCTGTAT CAGTGCAATG GGATCGCCTG GAAAGCCTGG 
AGTCCCCAAA CCAAGGATGT GGAAGACAAA TCCTGTCCAG CCGGGTGGCA CCAGCACTCA 
GGCTACTGTC ACATCTTGAT CACAGAGCAG AAAGGCACCT GGAATGCGGC TGCCCAAGCT 
TGCAGGGAAC AATACCTGGG CAACCTTGTA ACTGTATTCT CCAGGCAGCA CATGCGGTGG 
CTCTGGGACA TTGGTGGGAG AAAGTCCTTT TGGATAGGTT TGAACGACCA AGTGCATGCT 
GGCCACTGGG AGTGGATCGG TGGTGAACCT GTTGCCTTCA CCAATGGGAG AAGAGGGCCC 
TCTCAACGCT CCAAGCTTGG AAAGAGCTGT GTTTTGGTTC AAAGACAAGG GAAATGGCAA 
ACAAAAGACT GTAGGAGAGC CAAACCTCAT AATTATGTGT GTTCCAGAAA ACTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016869813.1
CDS	1065..7481
Translation	MNSLSWGAANAVLLLLLLAWASPTFISINRGVRVMKGHSAFLSGDDLKFAIPKEKDACKVEVVMNEPITQRVGKLTPQVFDCHFLPNEVKYVHNGCPILDEDTVKLRLYRFTERDTFIETFILWVYLLEPDCNIIHMSNNVLEVPEFNGLSQAIDKNLLRFDYDRMASLECTVSLDTARTRLPAHGQMVLGEPRPEEPRGDQPHSFFPESRMMSFNAFLELRAKLKCPGGSCTPGLKKIGSLKVSCEEFLLMGLRYQHLDPPSPNIDYISIQLDLTDTRSKIVYKSESAWLPVYIRAGIPNQIPKAAFMAVFILEVDQFILTSLTTSVLDCEEDETPKPLLVFNITKAPLQGYVTHLLDHTRPISSFTWKDLSDMQIAYQPPNSSHSERRHDEVELEVYDFFFERSAPMTVHISIRTADTNAPRVSWNTGLSLLEGQSRAITWEQFQVVDNDDIGAVRLVTVGGLQHGWLTLRGGKGFLFTVADLQAGVVRYHHDDSDSTKDFVVFRIFDGHHSIRHKFPINVLPKDDSPPFLITNVVIELEEGQTILIQGSMLRASDVDASDDYIFFNITKPPQAGEIMKKPGPGLIGYPVHGFLQRDLFNGIIYYRHFGGEIFEDSFQFVLWDSHEPPNLSVPQVATIHITPVDDQLPKEAPGVSRHLVVKETEVAYITKKQLHFIDSESYDRELVYTITTPPFFSFSHRHLDAGKLFMVDSIPKVVKNPTALELRSFTQHAVNYMKVAYMPPMQDIGPHCRDVQFTFSVSNQHGGTLHGICFNITILPVDNQVPEAFTNPLKVTEGGQSIISTEHILISDADTKLDNIDLSLRELPLHGRVELNGFPLNSGGTFSWGDLHTLKVRYQHDGTEVLQDDLLLEVTDGTNSAEFVLHVEVFPVNDEPPVLKADLMPVMNCSEGGEVVITSEYIFATDVDSDNLKLMFVIAREPQHGVVRRAGVTVDQFSQRDVISEAVTYKHTGGEIGLMPCFDTITLVVSDGEAGPFVNGCCYNGPNPSVPLHASFPVYDLNITVYPVDNQPPSIAIGPVFVVDEGCSTALTVNHLSATDPDTAADDLEFVLVSPPQFGYLENILPSVGFEKSNIGISIDSFQWKDMNAFHINYVQSRHLRIEPTADQFTVYVTDGKHHSLEIPFSIIINPTNDEAPDFVVQNITVCEGQMKELDSSIISAVDLDIPQDALLFSITQKPRHGLLIDRGFSKDFSENKQPANPHQKHAPVHSFSMELLKTGMRLTYMHDDSESLADDFTIQLSDGKHKILKTISVEVIPVNDEKPMLSKKAEIAMNMGETRIISSAILSAIDEDSPREKIYYVFERLPQNGQLQLKIGRDWVPLSPGMKCTQEEVDLNLLRYTHTGAMDSQNQDSFTFYLWDGNNRSPALDCQITIKDMEKGDIVILTKPLVVSKGDRGFLTTTTLLAVDGTDKPEELLYVITSPPRYGQIEYVHYPGVPITNFSQMDVVGQTVCYVHKSKVTVSSDRFRFIISNGLRTEHGVFEITLETVDRALPVVTRNKGLRLAQGAVGLLSPDLLQLTDPDTPAENLTFLLVQLPQHGQLYLWGTGLLQHNFTQQDVDSKNVAYRHSGGDSQTDCFTFMATDGTNQGFIVNGRVWEEPVLFTIQVDQLDKTAPRITLLHSPSQVGLLKNGCYGIYITSRVLKASDPDTEDDQIIFKILQGPKHGHLENTTTGEFIHEKFSQKDLNSKTILYIINPSLEVNSDTVEFQIMDPTGNSATPQILELKWSHIEWSQTEYEVCENVGLLPLEIIRRGYSMDSAFVGIKVNQVSAAVGKDFTVIPSKLIQFDPGQCHPSYSSNQSKHSTWEKGIWHLLPPGSSSSTTSGSFHLERRPLPSSMQLAVIRGDTLRGFDSTDLSQRKLRTRGNGKTVRPSSVYRNGTDIIYNYHGIVSLKLEDDSFPTHKRKAKVSIISQPQKTIKVAELPQADKVESTTDSHFPRQDQLPSFPKNCTLELKGLFHFEEGIQKLYQCNGIAWKAWSPQTKDVEDKSCPAGWHQHSGYCHILITEQKGTWNAAAQACREQYLGNLVTVFSRQHMRWLWDIGGRKSFWIGLNDQVHAGHWEWIGGEPVAFTNGRRGPSQRSKLGKSCVLVQRQGKWQTKDCRRAKPHNYVCSRKL

Target ORF information:

RefSeq Version	XM_017014324.1
Organism	Homo sapiens(human)
Definition	Homo sapiens FRAS1 related extracellular matrix 1 (FREM1), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017014324.1

ORF Insert Sequence:

ATGAACTCTC TGAGTTGGGG GGCTGCGAAT GCCGTGCTGC TGCTGCTCCT CCTGGCCTGG 
GCCAGCCCCA CCTTCATCAG CATCAACCGC GGGGTGAGGG TGATGAAGGG CCACTCTGCC 
TTCCTGTCAG GAGATGACCT GAAGTTTGCC ATCCCTAAAG AGAAAGATGC CTGCAAAGTG 
GAAGTTGTGA TGAATGAGCC AATAACCCAG AGGGTTGGGA AACTCACTCC ACAGGTCTTT 
GACTGCCATT TCCTTCCCAA CGAAGTCAAG TATGTTCACA ATGGTTGTCC AATTCTTGAT 
GAAGACACAG TGAAGCTCAG ACTTTACAGA TTTACTGAAA GAGATACCTT CATAGAAACT 
TTTATCCTGT GGGTCTATCT CCTGGAACCA GACTGTAACA TCATCCATAT GAGTAACAAT 
GTGCTGGAGG TGCCTGAATT CAATGGCTTG TCCCAAGCGA TTGATAAAAA TCTGCTCAGA 
TTCGATTATG ATAGGATGGC TAGCCTGGAA TGTACCGTCA GCCTGGACAC TGCGAGAACT 
CGGCTGCCAG CCCATGGCCA GATGGTTCTC GGGGAGCCTC GACCAGAAGA ACCTCGTGGA 
GATCAGCCAC ACAGTTTTTT CCCAGAGTCC CGTATGATGT CTTTTAATGC CTTTCTGGAA 
CTGAGAGCAA AACTGAAGTG TCCAGGTGGG AGCTGTACCC CAGGATTAAA GAAAATAGGA 
AGCCTCAAAG TGAGCTGTGA GGAGTTCCTG CTGATGGGCC TTCGTTATCA GCATCTGGAT 
CCCCCTTCAC CCAACATTGA TTATATCTCC ATCCAACTGG ACCTCACAGA TACCAGGAGC 
AAAATTGTGT ACAAGTCAGA GAGTGCGTGG CTGCCTGTCT ATATCAGAGC TGGAATTCCG 
AATCAGATTC CAAAGGCTGC ATTCATGGCC GTGTTTATTC TGGAAGTGGA TCAGTTCATC 
CTGACCTCCT TGACTACATC AGTTCTGGAC TGTGAAGAAG ATGAGACCCC TAAACCCTTG 
CTGGTGTTCA ACATTACTAA AGCCCCGCTC CAGGGCTATG TGACTCACCT GTTGGATCAC 
ACCAGACCAA TCTCCTCATT CACCTGGAAA GATCTCAGTG ACATGCAGAT CGCCTATCAG 
CCACCAAACA GCAGCCATTC TGAGAGGAGA CATGATGAGG TAGAATTGGA GGTATACGAC 
TTCTTCTTTG AAAGGAGTGC ACCTATGACA GTCCACATCT CCATCAGAAC AGCAGATACA 
AATGCCCCCC GTGTATCCTG GAATACAGGT CTGAGTCTCC TTGAGGGGCA GTCTCGAGCC 
ATCACTTGGG AACAGTTTCA GGTTGTCGAC AATGACGACA TTGGTGCTGT CCGGCTAGTC 
ACCGTTGGTG GCCTGCAGCA TGGATGGCTG ACTTTAAGAG GGGGGAAAGG GTTTCTCTTC 
ACCGTGGCTG ACCTCCAGGC TGGAGTTGTT CGCTATCATC ATGATGACAG CGACTCCACC 
AAAGACTTCG TGGTCTTCCG GATATTTGAT GGCCATCACA GCATCCGTCA CAAATTCCCC 
ATCAACGTCT TGCCCAAAGA TGATAGTCCC CCGTTCCTCA TAACCAATGT TGTGATTGAA 
CTGGAGGAGG GGCAGACCAT CCTGATCCAG GGATCCATGC TGCGAGCTTC AGATGTGGAC 
GCCAGTGATG ACTACATCTT CTTCAATATC ACAAAGCCTC CACAGGCTGG GGAGATCATG 
AAGAAGCCAG GGCCAGGACT GATAGGCTAT CCTGTCCATG GCTTCCTTCA GAGGGATTTG 
TTTAATGGAA TCATTTATTA TCGTCATTTT GGTGGAGAAA TCTTTGAAGA TTCTTTTCAA 
TTTGTCCTGT GGGACAGCCA TGAACCTCCA AATCTTTCAG TGCCACAGGT GGCAACAATC 
CATATAACTC CAGTGGATGA CCAGCTTCCA AAAGAGGCTC CTGGAGTTTC TCGGCATTTG 
GTTGTCAAGG AAACTGAGGT GGCCTATATA ACTAAGAAAC AGCTACATTT TATAGATTCA 
GAATCATATG ACAGGGAGCT GGTCTACACA ATAACTACTC CTCCATTTTT CTCCTTCAGC 
CACAGACACT TGGATGCTGG GAAATTATTT ATGGTGGACA GCATACCAAA AGTAGTTAAG 
AATCCTACGG CCCTGGAGCT GAGGTCATTC ACTCAGCATG CTGTGAACTA TATGAAAGTG 
GCCTACATGC CCCCCATGCA AGACATTGGT CCCCATTGCA GAGATGTCCA GTTCACATTT 
TCTGTCAGTA ACCAACATGG CGGTACTTTG CATGGGATCT GCTTTAACAT TACAATCCTC 
CCAGTGGACA ATCAAGTTCC TGAGGCGTTC ACCAACCCTC TGAAAGTGAC TGAGGGAGGT 
CAAAGCATCA TCAGCACAGA GCACATTCTA ATTTCTGATG CAGATACCAA GCTGGACAAT 
ATTGACCTCT CCCTGCGGGA ATTGCCTCTG CACGGAAGGG TGGAGCTGAA TGGATTTCCT 
CTAAATTCAG GGGGCACATT TTCTTGGGGC GATCTCCATA CCTTAAAAGT TAGGTATCAA 
CATGATGGAA CTGAAGTTCT TCAGGATGAC CTACTCTTGG AGGTCACCGA TGGCACAAAT 
TCAGCAGAAT TTGTACTACA TGTTGAGGTA TTCCCTGTCA ACGATGAGCC ACCAGTCTTA 
AAGGCTGACC TCATGCCTGT CATGAATTGC TCAGAGGGAG GAGAGGTGGT CATCACCTCT 
GAATACATTT TTGCTACTGA TGTGGACAGC GATAACTTGA AGTTGATGTT TGTGATTGCT 
CGCGAACCTC AGCATGGGGT GGTGAGGAGA GCTGGAGTCA CAGTGGATCA GTTCTCTCAG 
AGAGATGTTA TCTCAGAGGC CGTGACATAC AAACACACAG GTGGCGAGAT TGGCCTGATG 
CCTTGTTTTG ACACCATTAC ATTGGTGGTT TCGGATGGAG AGGCTGGCCC TTTTGTGAAT 
GGCTGTTGCT ACAATGGACC CAATCCATCT GTTCCACTTC ATGCGTCCTT TCCAGTGTAT 
GATCTCAACA TCACGGTATA CCCAGTAGAC AACCAGCCAC CTTCCATTGC AATAGGTCCC 
GTGTTTGTTG TAGATGAGGG CTGCTCAACA GCCCTTACTG TTAACCATTT GTCCGCCACT 
GACCCTGACA CTGCAGCAGA TGACTTGGAA TTTGTTTTGG TTTCTCCTCC TCAGTTTGGC 
TACCTCGAAA ATATACTCCC TTCTGTGGGT TTTGAAAAAA GCAATATTGG CATAAGTATA 
GATTCATTTC AGTGGAAAGA CATGAACGCT TTTCACATTA ACTATGTGCA GTCCAGGCAT 
CTGAGGATAG AACCAACTGC CGACCAGTTC ACGGTGTACG TCACAGATGG GAAGCATCAC 
TCCTTGGAGA TACCATTTTC TATTATAATC AACCCCACAA ATGATGAAGC TCCTGACTTT 
GTAGTGCAGA ATATTACTGT GTGTGAGGGT CAGATGAAAG AGCTGGACTC TTCCATCATC 
AGCGCTGTGG ACCTGGACAT TCCCCAGGAT GCCCTGCTGT TCAGCATCAC TCAAAAGCCA 
CGCCATGGCC TCCTCATCGA TAGGGGGTTT AGCAAAGACT TCTCTGAGAA TAAGCAGCCA 
GCCAACCCTC ACCAGAAACA TGCACCTGTT CACAGCTTTT CCATGGAACT CCTCAAGACT 
GGAATGAGGT TGACGTACAT GCATGATGAC TCAGAGAGCC TTGCTGATGA TTTTACAATC 
CAATTGTCAG ATGGGAAACA TAAGATACTT AAAACCATTT CAGTAGAGGT CATCCCAGTT 
AATGATGAAA AACCAATGCT GAGCAAGAAG GCTGAAATTG CAATGAATAT GGGTGAAACT 
CGTATTATTT CCAGTGCTAT TCTTTCAGCC ATAGATGAAG ACTCACCCAG GGAGAAGATT 
TACTATGTAT TTGAAAGGCT TCCCCAAAAT GGGCAACTTC AGCTTAAGAT AGGGAGGGAC 
TGGGTTCCTC TCTCCCCTGG CATGAAATGC ACTCAGGAGG AAGTGGATCT GAACTTGCTG 
AGATACACAC ACACCGGGGC AATGGATTCC CAGAATCAAG ATAGCTTCAC CTTCTACCTT 
TGGGATGGCA ACAACAGGTC CCCTGCTCTT GACTGTCAAA TCACCATCAA GGACATGGAA 
AAAGGTGATA TTGTCATCCT TACAAAACCA CTCGTGGTGT CTAAAGGTGA TAGAGGTTTC 
TTAACAACCA CCACGCTCCT GGCTGTGGAC GGAACAGACA AGCCTGAGGA ACTGCTCTAT 
GTCATCACCT CCCCTCCGCG ATATGGCCAG ATCGAATATG TTCACTATCC TGGAGTTCCC 
ATTACAAACT TCAGCCAAAT GGATGTAGTG GGGCAGACAG TTTGCTATGT ACACAAGAGC 
AAGGTGACTG TCTCCAGTGA CAGATTCAGA TTCATCATCA GCAATGGACT GCGGACCGAG 
CACGGGGTGT TTGAGATCAC ACTGGAGACT GTGGACAGAG CCCTGCCTGT GGTAACCAGG 
AACAAGGGGT TGAGACTGGC CCAAGGGGCC GTGGGCCTGC TTTCCCCTGA CCTCCTTCAG 
CTGACCGACC CTGATACACC TGCGGAGAAC CTCACCTTCC TCTTGGTTCA GCTCCCCCAG 
CATGGCCAGC TCTACCTGTG GGGGACAGGG CTACTTCAAC ACAATTTCAC CCAGCAGGAT 
GTGGACAGCA AGAATGTGGC CTATCGGCAC TCAGGAGGGG ACTCCCAGAC TGACTGCTTT 
ACTTTCATGG CCACAGATGG GACAAACCAA GGCTTTATTG TGAATGGGAG AGTGTGGGAA 
GAACCTGTTT TATTCACCAT TCAGGTAGAC CAATTGGACA AAACAGCTCC TCGTATCACA 
CTCTTGCATT CCCCTTCTCA AGTGGGGCTC CTGAAAAATG GCTGCTACGG GATTTACATC 
ACTTCCCGCG TGTTGAAGGC ATCAGACCCT GACACTGAGG ACGATCAGAT CATCTTTAAA 
ATTCTACAAG GCCCAAAACA TGGACATCTG GAGAACACAA CAACAGGTGA ATTTATCCAT 
GAGAAATTTA GCCAAAAGGA CTTAAACAGT AAGACTATTC TTTACATCAT AAACCCATCT 
TTGGAAGTAA ATTCAGATAC CGTGGAATTT CAAATCATGG ACCCCACAGG GAACTCGGCC 
ACTCCTCAAA TTTTGGAACT GAAGTGGTCT CATATTGAAT GGTCACAGAC CGAATATGAA 
GTCTGTGAGA ATGTGGGTTT GTTGCCCTTG GAAATTATCA GAAGGGGATA TTCCATGGAC 
TCGGCCTTTG TGGGTATAAA GGTCAACCAA GTGTCAGCTG CAGTTGGAAA AGATTTCACC 
GTGATTCCAT CTAAACTGAT TCAGTTTGAC CCAGGACAAT GCCATCCTTC ATATTCCTCC 
AACCAAAGCA AGCACAGCAC ATGGGAGAAG GGCATTTGGC ATCTGCTGCC CCCAGGGTCT 
TCCTCATCCA CCACTTCTGG TTCCTTTCAT CTGGAAAGAA GACCTCTTCC ATCTTCCATG 
CAGCTAGCAG TCATCAGGGG AGACACCCTG CGGGGCTTTG ATTCTACAGA TCTTTCTCAA 
AGGAAGCTTA GGACCCGTGG GAATGGCAAA ACAGTTCGTC CATCCTCTGT TTATAGAAAT 
GGAACAGACA TCATCTATAA TTATCATGGG ATAGTTTCCT TGAAACTGGA GGATGACAGT 
TTCCCAACTC ACAAAAGGAA GGCCAAAGTA TCCATCATTA GTCAGCCACA AAAGACAATC 
AAAGTGGCAG AACTGCCTCA AGCAGATAAG GTGGAATCCA CAACTGACTC ACACTTCCCC 
AGACAGGACC AGTTGCCCTC ATTTCCAAAG AACTGCACTC TGGAATTAAA GGGACTCTTC 
CATTTTGAAG AAGGCATCCA GAAGCTGTAT CAGTGCAATG GGATCGCCTG GAAAGCCTGG 
AGTCCCCAAA CCAAGGATGT GGAAGACAAA TCCTGTCCAG CCGGGTGGCA CCAGCACTCA 
GGCTACTGTC ACATCTTGAT CACAGAGCAG AAAGGCACCT GGAATGCGGC TGCCCAAGCT 
TGCAGGGAAC AATACCTGGG CAACCTTGTA ACTGTATTCT CCAGGCAGCA CATGCGGTGG 
CTCTGGGACA TTGGTGGGAG AAAGTCCTTT TGGATAGGTT TGAACGACCA AGTGCATGCT 
GGCCACTGGG AGTGGATCGG TGGTGAACCT GTTGCCTTCA CCAATGGGAG AAGAGGGCCC 
TCTCAACGCT CCAAGCTTGG AAAGAGCTGT GTTTTGGTTC AAAGACAAGG GAAATGGCAA 
ACAAAAGACT GTAGGAGAGC CAAACCTCAT AATTATGTGT GTTCCAGAAA ACTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FREM1 ( XM_017014324.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016869813.1 Homo sapiens FRAS1 related extracellular matrix 1 (FREM1), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OHu98460
Clone ID Related Accession (Same CDS sequence)	XM_017014324.1 , XM_017014324.2
Accession Version	XM_017014324.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6417bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	FRAS1-related extracellular matrix protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017014324.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACTCTC TGAGTTGGGG GGCTGCGAAT GCCGTGCTGC TGCTGCTCCT CCTGGCCTGG 
GCCAGCCCCA CCTTCATCAG CATCAACCGC GGGGTGAGGG TGATGAAGGG CCACTCTGCC 
TTCCTGTCAG GAGATGACCT GAAGTTTGCC ATCCCTAAAG AGAAAGATGC CTGCAAAGTG 
GAAGTTGTGA TGAATGAGCC AATAACCCAG AGGGTTGGGA AACTCACTCC ACAGGTCTTT 
GACTGCCATT TCCTTCCCAA CGAAGTCAAG TATGTTCACA ATGGTTGTCC AATTCTTGAT 
GAAGACACAG TGAAGCTCAG ACTTTACAGA TTTACTGAAA GAGATACCTT CATAGAAACT 
TTTATCCTGT GGGTCTATCT CCTGGAACCA GACTGTAACA TCATCCATAT GAGTAACAAT 
GTGCTGGAGG TGCCTGAATT CAATGGCTTG TCCCAAGCGA TTGATAAAAA TCTGCTCAGA 
TTCGATTATG ATAGGATGGC TAGCCTGGAA TGTACCGTCA GCCTGGACAC TGCGAGAACT 
CGGCTGCCAG CCCATGGCCA GATGGTTCTC GGGGAGCCTC GACCAGAAGA ACCTCGTGGA 
GATCAGCCAC ACAGTTTTTT CCCAGAGTCC CGTATGATGT CTTTTAATGC CTTTCTGGAA 
CTGAGAGCAA AACTGAAGTG TCCAGGTGGG AGCTGTACCC CAGGATTAAA GAAAATAGGA 
AGCCTCAAAG TGAGCTGTGA GGAGTTCCTG CTGATGGGCC TTCGTTATCA GCATCTGGAT 
CCCCCTTCAC CCAACATTGA TTATATCTCC ATCCAACTGG ACCTCACAGA TACCAGGAGC 
AAAATTGTGT ACAAGTCAGA GAGTGCGTGG CTGCCTGTCT ATATCAGAGC TGGAATTCCG 
AATCAGATTC CAAAGGCTGC ATTCATGGCC GTGTTTATTC TGGAAGTGGA TCAGTTCATC 
CTGACCTCCT TGACTACATC AGTTCTGGAC TGTGAAGAAG ATGAGACCCC TAAACCCTTG 
CTGGTGTTCA ACATTACTAA AGCCCCGCTC CAGGGCTATG TGACTCACCT GTTGGATCAC 
ACCAGACCAA TCTCCTCATT CACCTGGAAA GATCTCAGTG ACATGCAGAT CGCCTATCAG 
CCACCAAACA GCAGCCATTC TGAGAGGAGA CATGATGAGG TAGAATTGGA GGTATACGAC 
TTCTTCTTTG AAAGGAGTGC ACCTATGACA GTCCACATCT CCATCAGAAC AGCAGATACA 
AATGCCCCCC GTGTATCCTG GAATACAGGT CTGAGTCTCC TTGAGGGGCA GTCTCGAGCC 
ATCACTTGGG AACAGTTTCA GGTTGTCGAC AATGACGACA TTGGTGCTGT CCGGCTAGTC 
ACCGTTGGTG GCCTGCAGCA TGGATGGCTG ACTTTAAGAG GGGGGAAAGG GTTTCTCTTC 
ACCGTGGCTG ACCTCCAGGC TGGAGTTGTT CGCTATCATC ATGATGACAG CGACTCCACC 
AAAGACTTCG TGGTCTTCCG GATATTTGAT GGCCATCACA GCATCCGTCA CAAATTCCCC 
ATCAACGTCT TGCCCAAAGA TGATAGTCCC CCGTTCCTCA TAACCAATGT TGTGATTGAA 
CTGGAGGAGG GGCAGACCAT CCTGATCCAG GGATCCATGC TGCGAGCTTC AGATGTGGAC 
GCCAGTGATG ACTACATCTT CTTCAATATC ACAAAGCCTC CACAGGCTGG GGAGATCATG 
AAGAAGCCAG GGCCAGGACT GATAGGCTAT CCTGTCCATG GCTTCCTTCA GAGGGATTTG 
TTTAATGGAA TCATTTATTA TCGTCATTTT GGTGGAGAAA TCTTTGAAGA TTCTTTTCAA 
TTTGTCCTGT GGGACAGCCA TGAACCTCCA AATCTTTCAG TGCCACAGGT GGCAACAATC 
CATATAACTC CAGTGGATGA CCAGCTTCCA AAAGAGGCTC CTGGAGTTTC TCGGCATTTG 
GTTGTCAAGG AAACTGAGGT GGCCTATATA ACTAAGAAAC AGCTACATTT TATAGATTCA 
GAATCATATG ACAGGGAGCT GGTCTACACA ATAACTACTC CTCCATTTTT CTCCTTCAGC 
CACAGACACT TGGATGCTGG GAAATTATTT ATGGTGGACA GCATACCAAA AGTAGTTAAG 
AATCCTACGG CCCTGGAGCT GAGGTCATTC ACTCAGCATG CTGTGAACTA TATGAAAGTG 
GCCTACATGC CCCCCATGCA AGACATTGGT CCCCATTGCA GAGATGTCCA GTTCACATTT 
TCTGTCAGTA ACCAACATGG CGGTACTTTG CATGGGATCT GCTTTAACAT TACAATCCTC 
CCAGTGGACA ATCAAGTTCC TGAGGCGTTC ACCAACCCTC TGAAAGTGAC TGAGGGAGGT 
CAAAGCATCA TCAGCACAGA GCACATTCTA ATTTCTGATG CAGATACCAA GCTGGACAAT 
ATTGACCTCT CCCTGCGGGA ATTGCCTCTG CACGGAAGGG TGGAGCTGAA TGGATTTCCT 
CTAAATTCAG GGGGCACATT TTCTTGGGGC GATCTCCATA CCTTAAAAGT TAGGTATCAA 
CATGATGGAA CTGAAGTTCT TCAGGATGAC CTACTCTTGG AGGTCACCGA TGGCACAAAT 
TCAGCAGAAT TTGTACTACA TGTTGAGGTA TTCCCTGTCA ACGATGAGCC ACCAGTCTTA 
AAGGCTGACC TCATGCCTGT CATGAATTGC TCAGAGGGAG GAGAGGTGGT CATCACCTCT 
GAATACATTT TTGCTACTGA TGTGGACAGC GATAACTTGA AGTTGATGTT TGTGATTGCT 
CGCGAACCTC AGCATGGGGT GGTGAGGAGA GCTGGAGTCA CAGTGGATCA GTTCTCTCAG 
AGAGATGTTA TCTCAGAGGC CGTGACATAC AAACACACAG GTGGCGAGAT TGGCCTGATG 
CCTTGTTTTG ACACCATTAC ATTGGTGGTT TCGGATGGAG AGGCTGGCCC TTTTGTGAAT 
GGCTGTTGCT ACAATGGACC CAATCCATCT GTTCCACTTC ATGCGTCCTT TCCAGTGTAT 
GATCTCAACA TCACGGTATA CCCAGTAGAC AACCAGCCAC CTTCCATTGC AATAGGTCCC 
GTGTTTGTTG TAGATGAGGG CTGCTCAACA GCCCTTACTG TTAACCATTT GTCCGCCACT 
GACCCTGACA CTGCAGCAGA TGACTTGGAA TTTGTTTTGG TTTCTCCTCC TCAGTTTGGC 
TACCTCGAAA ATATACTCCC TTCTGTGGGT TTTGAAAAAA GCAATATTGG CATAAGTATA 
GATTCATTTC AGTGGAAAGA CATGAACGCT TTTCACATTA ACTATGTGCA GTCCAGGCAT 
CTGAGGATAG AACCAACTGC CGACCAGTTC ACGGTGTACG TCACAGATGG GAAGCATCAC 
TCCTTGGAGA TACCATTTTC TATTATAATC AACCCCACAA ATGATGAAGC TCCTGACTTT 
GTAGTGCAGA ATATTACTGT GTGTGAGGGT CAGATGAAAG AGCTGGACTC TTCCATCATC 
AGCGCTGTGG ACCTGGACAT TCCCCAGGAT GCCCTGCTGT TCAGCATCAC TCAAAAGCCA 
CGCCATGGCC TCCTCATCGA TAGGGGGTTT AGCAAAGACT TCTCTGAGAA TAAGCAGCCA 
GCCAACCCTC ACCAGAAACA TGCACCTGTT CACAGCTTTT CCATGGAACT CCTCAAGACT 
GGAATGAGGT TGACGTACAT GCATGATGAC TCAGAGAGCC TTGCTGATGA TTTTACAATC 
CAATTGTCAG ATGGGAAACA TAAGATACTT AAAACCATTT CAGTAGAGGT CATCCCAGTT 
AATGATGAAA AACCAATGCT GAGCAAGAAG GCTGAAATTG CAATGAATAT GGGTGAAACT 
CGTATTATTT CCAGTGCTAT TCTTTCAGCC ATAGATGAAG ACTCACCCAG GGAGAAGATT 
TACTATGTAT TTGAAAGGCT TCCCCAAAAT GGGCAACTTC AGCTTAAGAT AGGGAGGGAC 
TGGGTTCCTC TCTCCCCTGG CATGAAATGC ACTCAGGAGG AAGTGGATCT GAACTTGCTG 
AGATACACAC ACACCGGGGC AATGGATTCC CAGAATCAAG ATAGCTTCAC CTTCTACCTT 
TGGGATGGCA ACAACAGGTC CCCTGCTCTT GACTGTCAAA TCACCATCAA GGACATGGAA 
AAAGGTGATA TTGTCATCCT TACAAAACCA CTCGTGGTGT CTAAAGGTGA TAGAGGTTTC 
TTAACAACCA CCACGCTCCT GGCTGTGGAC GGAACAGACA AGCCTGAGGA ACTGCTCTAT 
GTCATCACCT CCCCTCCGCG ATATGGCCAG ATCGAATATG TTCACTATCC TGGAGTTCCC 
ATTACAAACT TCAGCCAAAT GGATGTAGTG GGGCAGACAG TTTGCTATGT ACACAAGAGC 
AAGGTGACTG TCTCCAGTGA CAGATTCAGA TTCATCATCA GCAATGGACT GCGGACCGAG 
CACGGGGTGT TTGAGATCAC ACTGGAGACT GTGGACAGAG CCCTGCCTGT GGTAACCAGG 
AACAAGGGGT TGAGACTGGC CCAAGGGGCC GTGGGCCTGC TTTCCCCTGA CCTCCTTCAG 
CTGACCGACC CTGATACACC TGCGGAGAAC CTCACCTTCC TCTTGGTTCA GCTCCCCCAG 
CATGGCCAGC TCTACCTGTG GGGGACAGGG CTACTTCAAC ACAATTTCAC CCAGCAGGAT 
GTGGACAGCA AGAATGTGGC CTATCGGCAC TCAGGAGGGG ACTCCCAGAC TGACTGCTTT 
ACTTTCATGG CCACAGATGG GACAAACCAA GGCTTTATTG TGAATGGGAG AGTGTGGGAA 
GAACCTGTTT TATTCACCAT TCAGGTAGAC CAATTGGACA AAACAGCTCC TCGTATCACA 
CTCTTGCATT CCCCTTCTCA AGTGGGGCTC CTGAAAAATG GCTGCTACGG GATTTACATC 
ACTTCCCGCG TGTTGAAGGC ATCAGACCCT GACACTGAGG ACGATCAGAT CATCTTTAAA 
ATTCTACAAG GCCCAAAACA TGGACATCTG GAGAACACAA CAACAGGTGA ATTTATCCAT 
GAGAAATTTA GCCAAAAGGA CTTAAACAGT AAGACTATTC TTTACATCAT AAACCCATCT 
TTGGAAGTAA ATTCAGATAC CGTGGAATTT CAAATCATGG ACCCCACAGG GAACTCGGCC 
ACTCCTCAAA TTTTGGAACT GAAGTGGTCT CATATTGAAT GGTCACAGAC CGAATATGAA 
GTCTGTGAGA ATGTGGGTTT GTTGCCCTTG GAAATTATCA GAAGGGGATA TTCCATGGAC 
TCGGCCTTTG TGGGTATAAA GGTCAACCAA GTGTCAGCTG CAGTTGGAAA AGATTTCACC 
GTGATTCCAT CTAAACTGAT TCAGTTTGAC CCAGGACAAT GCCATCCTTC ATATTCCTCC 
AACCAAAGCA AGCACAGCAC ATGGGAGAAG GGCATTTGGC ATCTGCTGCC CCCAGGGTCT 
TCCTCATCCA CCACTTCTGG TTCCTTTCAT CTGGAAAGAA GACCTCTTCC ATCTTCCATG 
CAGCTAGCAG TCATCAGGGG AGACACCCTG CGGGGCTTTG ATTCTACAGA TCTTTCTCAA 
AGGAAGCTTA GGACCCGTGG GAATGGCAAA ACAGTTCGTC CATCCTCTGT TTATAGAAAT 
GGAACAGACA TCATCTATAA TTATCATGGG ATAGTTTCCT TGAAACTGGA GGATGACAGT 
TTCCCAACTC ACAAAAGGAA GGCCAAAGTA TCCATCATTA GTCAGCCACA AAAGACAATC 
AAAGTGGCAG AACTGCCTCA AGCAGATAAG GTGGAATCCA CAACTGACTC ACACTTCCCC 
AGACAGGACC AGTTGCCCTC ATTTCCAAAG AACTGCACTC TGGAATTAAA GGGACTCTTC 
CATTTTGAAG AAGGCATCCA GAAGCTGTAT CAGTGCAATG GGATCGCCTG GAAAGCCTGG 
AGTCCCCAAA CCAAGGATGT GGAAGACAAA TCCTGTCCAG CCGGGTGGCA CCAGCACTCA 
GGCTACTGTC ACATCTTGAT CACAGAGCAG AAAGGCACCT GGAATGCGGC TGCCCAAGCT 
TGCAGGGAAC AATACCTGGG CAACCTTGTA ACTGTATTCT CCAGGCAGCA CATGCGGTGG 
CTCTGGGACA TTGGTGGGAG AAAGTCCTTT TGGATAGGTT TGAACGACCA AGTGCATGCT 
GGCCACTGGG AGTGGATCGG TGGTGAACCT GTTGCCTTCA CCAATGGGAG AAGAGGGCCC 
TCTCAACGCT CCAAGCTTGG AAAGAGCTGT GTTTTGGTTC AAAGACAAGG GAAATGGCAA 
ACAAAAGACT GTAGGAGAGC CAAACCTCAT AATTATGTGT GTTCCAGAAA ACTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016869813.1
CDS	787..7203
Translation	MNSLSWGAANAVLLLLLLAWASPTFISINRGVRVMKGHSAFLSGDDLKFAIPKEKDACKVEVVMNEPITQRVGKLTPQVFDCHFLPNEVKYVHNGCPILDEDTVKLRLYRFTERDTFIETFILWVYLLEPDCNIIHMSNNVLEVPEFNGLSQAIDKNLLRFDYDRMASLECTVSLDTARTRLPAHGQMVLGEPRPEEPRGDQPHSFFPESRMMSFNAFLELRAKLKCPGGSCTPGLKKIGSLKVSCEEFLLMGLRYQHLDPPSPNIDYISIQLDLTDTRSKIVYKSESAWLPVYIRAGIPNQIPKAAFMAVFILEVDQFILTSLTTSVLDCEEDETPKPLLVFNITKAPLQGYVTHLLDHTRPISSFTWKDLSDMQIAYQPPNSSHSERRHDEVELEVYDFFFERSAPMTVHISIRTADTNAPRVSWNTGLSLLEGQSRAITWEQFQVVDNDDIGAVRLVTVGGLQHGWLTLRGGKGFLFTVADLQAGVVRYHHDDSDSTKDFVVFRIFDGHHSIRHKFPINVLPKDDSPPFLITNVVIELEEGQTILIQGSMLRASDVDASDDYIFFNITKPPQAGEIMKKPGPGLIGYPVHGFLQRDLFNGIIYYRHFGGEIFEDSFQFVLWDSHEPPNLSVPQVATIHITPVDDQLPKEAPGVSRHLVVKETEVAYITKKQLHFIDSESYDRELVYTITTPPFFSFSHRHLDAGKLFMVDSIPKVVKNPTALELRSFTQHAVNYMKVAYMPPMQDIGPHCRDVQFTFSVSNQHGGTLHGICFNITILPVDNQVPEAFTNPLKVTEGGQSIISTEHILISDADTKLDNIDLSLRELPLHGRVELNGFPLNSGGTFSWGDLHTLKVRYQHDGTEVLQDDLLLEVTDGTNSAEFVLHVEVFPVNDEPPVLKADLMPVMNCSEGGEVVITSEYIFATDVDSDNLKLMFVIAREPQHGVVRRAGVTVDQFSQRDVISEAVTYKHTGGEIGLMPCFDTITLVVSDGEAGPFVNGCCYNGPNPSVPLHASFPVYDLNITVYPVDNQPPSIAIGPVFVVDEGCSTALTVNHLSATDPDTAADDLEFVLVSPPQFGYLENILPSVGFEKSNIGISIDSFQWKDMNAFHINYVQSRHLRIEPTADQFTVYVTDGKHHSLEIPFSIIINPTNDEAPDFVVQNITVCEGQMKELDSSIISAVDLDIPQDALLFSITQKPRHGLLIDRGFSKDFSENKQPANPHQKHAPVHSFSMELLKTGMRLTYMHDDSESLADDFTIQLSDGKHKILKTISVEVIPVNDEKPMLSKKAEIAMNMGETRIISSAILSAIDEDSPREKIYYVFERLPQNGQLQLKIGRDWVPLSPGMKCTQEEVDLNLLRYTHTGAMDSQNQDSFTFYLWDGNNRSPALDCQITIKDMEKGDIVILTKPLVVSKGDRGFLTTTTLLAVDGTDKPEELLYVITSPPRYGQIEYVHYPGVPITNFSQMDVVGQTVCYVHKSKVTVSSDRFRFIISNGLRTEHGVFEITLETVDRALPVVTRNKGLRLAQGAVGLLSPDLLQLTDPDTPAENLTFLLVQLPQHGQLYLWGTGLLQHNFTQQDVDSKNVAYRHSGGDSQTDCFTFMATDGTNQGFIVNGRVWEEPVLFTIQVDQLDKTAPRITLLHSPSQVGLLKNGCYGIYITSRVLKASDPDTEDDQIIFKILQGPKHGHLENTTTGEFIHEKFSQKDLNSKTILYIINPSLEVNSDTVEFQIMDPTGNSATPQILELKWSHIEWSQTEYEVCENVGLLPLEIIRRGYSMDSAFVGIKVNQVSAAVGKDFTVIPSKLIQFDPGQCHPSYSSNQSKHSTWEKGIWHLLPPGSSSSTTSGSFHLERRPLPSSMQLAVIRGDTLRGFDSTDLSQRKLRTRGNGKTVRPSSVYRNGTDIIYNYHGIVSLKLEDDSFPTHKRKAKVSIISQPQKTIKVAELPQADKVESTTDSHFPRQDQLPSFPKNCTLELKGLFHFEEGIQKLYQCNGIAWKAWSPQTKDVEDKSCPAGWHQHSGYCHILITEQKGTWNAAAQACREQYLGNLVTVFSRQHMRWLWDIGGRKSFWIGLNDQVHAGHWEWIGGEPVAFTNGRRGPSQRSKLGKSCVLVQRQGKWQTKDCRRAKPHNYVCSRKL

Target ORF information:

RefSeq Version	XM_017014324.2
Organism	Homo sapiens(human)
Definition	Homo sapiens FRAS1 related extracellular matrix 1 (FREM1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017014324.2

ORF Insert Sequence:

ATGAACTCTC TGAGTTGGGG GGCTGCGAAT GCCGTGCTGC TGCTGCTCCT CCTGGCCTGG 
GCCAGCCCCA CCTTCATCAG CATCAACCGC GGGGTGAGGG TGATGAAGGG CCACTCTGCC 
TTCCTGTCAG GAGATGACCT GAAGTTTGCC ATCCCTAAAG AGAAAGATGC CTGCAAAGTG 
GAAGTTGTGA TGAATGAGCC AATAACCCAG AGGGTTGGGA AACTCACTCC ACAGGTCTTT 
GACTGCCATT TCCTTCCCAA CGAAGTCAAG TATGTTCACA ATGGTTGTCC AATTCTTGAT 
GAAGACACAG TGAAGCTCAG ACTTTACAGA TTTACTGAAA GAGATACCTT CATAGAAACT 
TTTATCCTGT GGGTCTATCT CCTGGAACCA GACTGTAACA TCATCCATAT GAGTAACAAT 
GTGCTGGAGG TGCCTGAATT CAATGGCTTG TCCCAAGCGA TTGATAAAAA TCTGCTCAGA 
TTCGATTATG ATAGGATGGC TAGCCTGGAA TGTACCGTCA GCCTGGACAC TGCGAGAACT 
CGGCTGCCAG CCCATGGCCA GATGGTTCTC GGGGAGCCTC GACCAGAAGA ACCTCGTGGA 
GATCAGCCAC ACAGTTTTTT CCCAGAGTCC CGTATGATGT CTTTTAATGC CTTTCTGGAA 
CTGAGAGCAA AACTGAAGTG TCCAGGTGGG AGCTGTACCC CAGGATTAAA GAAAATAGGA 
AGCCTCAAAG TGAGCTGTGA GGAGTTCCTG CTGATGGGCC TTCGTTATCA GCATCTGGAT 
CCCCCTTCAC CCAACATTGA TTATATCTCC ATCCAACTGG ACCTCACAGA TACCAGGAGC 
AAAATTGTGT ACAAGTCAGA GAGTGCGTGG CTGCCTGTCT ATATCAGAGC TGGAATTCCG 
AATCAGATTC CAAAGGCTGC ATTCATGGCC GTGTTTATTC TGGAAGTGGA TCAGTTCATC 
CTGACCTCCT TGACTACATC AGTTCTGGAC TGTGAAGAAG ATGAGACCCC TAAACCCTTG 
CTGGTGTTCA ACATTACTAA AGCCCCGCTC CAGGGCTATG TGACTCACCT GTTGGATCAC 
ACCAGACCAA TCTCCTCATT CACCTGGAAA GATCTCAGTG ACATGCAGAT CGCCTATCAG 
CCACCAAACA GCAGCCATTC TGAGAGGAGA CATGATGAGG TAGAATTGGA GGTATACGAC 
TTCTTCTTTG AAAGGAGTGC ACCTATGACA GTCCACATCT CCATCAGAAC AGCAGATACA 
AATGCCCCCC GTGTATCCTG GAATACAGGT CTGAGTCTCC TTGAGGGGCA GTCTCGAGCC 
ATCACTTGGG AACAGTTTCA GGTTGTCGAC AATGACGACA TTGGTGCTGT CCGGCTAGTC 
ACCGTTGGTG GCCTGCAGCA TGGATGGCTG ACTTTAAGAG GGGGGAAAGG GTTTCTCTTC 
ACCGTGGCTG ACCTCCAGGC TGGAGTTGTT CGCTATCATC ATGATGACAG CGACTCCACC 
AAAGACTTCG TGGTCTTCCG GATATTTGAT GGCCATCACA GCATCCGTCA CAAATTCCCC 
ATCAACGTCT TGCCCAAAGA TGATAGTCCC CCGTTCCTCA TAACCAATGT TGTGATTGAA 
CTGGAGGAGG GGCAGACCAT CCTGATCCAG GGATCCATGC TGCGAGCTTC AGATGTGGAC 
GCCAGTGATG ACTACATCTT CTTCAATATC ACAAAGCCTC CACAGGCTGG GGAGATCATG 
AAGAAGCCAG GGCCAGGACT GATAGGCTAT CCTGTCCATG GCTTCCTTCA GAGGGATTTG 
TTTAATGGAA TCATTTATTA TCGTCATTTT GGTGGAGAAA TCTTTGAAGA TTCTTTTCAA 
TTTGTCCTGT GGGACAGCCA TGAACCTCCA AATCTTTCAG TGCCACAGGT GGCAACAATC 
CATATAACTC CAGTGGATGA CCAGCTTCCA AAAGAGGCTC CTGGAGTTTC TCGGCATTTG 
GTTGTCAAGG AAACTGAGGT GGCCTATATA ACTAAGAAAC AGCTACATTT TATAGATTCA 
GAATCATATG ACAGGGAGCT GGTCTACACA ATAACTACTC CTCCATTTTT CTCCTTCAGC 
CACAGACACT TGGATGCTGG GAAATTATTT ATGGTGGACA GCATACCAAA AGTAGTTAAG 
AATCCTACGG CCCTGGAGCT GAGGTCATTC ACTCAGCATG CTGTGAACTA TATGAAAGTG 
GCCTACATGC CCCCCATGCA AGACATTGGT CCCCATTGCA GAGATGTCCA GTTCACATTT 
TCTGTCAGTA ACCAACATGG CGGTACTTTG CATGGGATCT GCTTTAACAT TACAATCCTC 
CCAGTGGACA ATCAAGTTCC TGAGGCGTTC ACCAACCCTC TGAAAGTGAC TGAGGGAGGT 
CAAAGCATCA TCAGCACAGA GCACATTCTA ATTTCTGATG CAGATACCAA GCTGGACAAT 
ATTGACCTCT CCCTGCGGGA ATTGCCTCTG CACGGAAGGG TGGAGCTGAA TGGATTTCCT 
CTAAATTCAG GGGGCACATT TTCTTGGGGC GATCTCCATA CCTTAAAAGT TAGGTATCAA 
CATGATGGAA CTGAAGTTCT TCAGGATGAC CTACTCTTGG AGGTCACCGA TGGCACAAAT 
TCAGCAGAAT TTGTACTACA TGTTGAGGTA TTCCCTGTCA ACGATGAGCC ACCAGTCTTA 
AAGGCTGACC TCATGCCTGT CATGAATTGC TCAGAGGGAG GAGAGGTGGT CATCACCTCT 
GAATACATTT TTGCTACTGA TGTGGACAGC GATAACTTGA AGTTGATGTT TGTGATTGCT 
CGCGAACCTC AGCATGGGGT GGTGAGGAGA GCTGGAGTCA CAGTGGATCA GTTCTCTCAG 
AGAGATGTTA TCTCAGAGGC CGTGACATAC AAACACACAG GTGGCGAGAT TGGCCTGATG 
CCTTGTTTTG ACACCATTAC ATTGGTGGTT TCGGATGGAG AGGCTGGCCC TTTTGTGAAT 
GGCTGTTGCT ACAATGGACC CAATCCATCT GTTCCACTTC ATGCGTCCTT TCCAGTGTAT 
GATCTCAACA TCACGGTATA CCCAGTAGAC AACCAGCCAC CTTCCATTGC AATAGGTCCC 
GTGTTTGTTG TAGATGAGGG CTGCTCAACA GCCCTTACTG TTAACCATTT GTCCGCCACT 
GACCCTGACA CTGCAGCAGA TGACTTGGAA TTTGTTTTGG TTTCTCCTCC TCAGTTTGGC 
TACCTCGAAA ATATACTCCC TTCTGTGGGT TTTGAAAAAA GCAATATTGG CATAAGTATA 
GATTCATTTC AGTGGAAAGA CATGAACGCT TTTCACATTA ACTATGTGCA GTCCAGGCAT 
CTGAGGATAG AACCAACTGC CGACCAGTTC ACGGTGTACG TCACAGATGG GAAGCATCAC 
TCCTTGGAGA TACCATTTTC TATTATAATC AACCCCACAA ATGATGAAGC TCCTGACTTT 
GTAGTGCAGA ATATTACTGT GTGTGAGGGT CAGATGAAAG AGCTGGACTC TTCCATCATC 
AGCGCTGTGG ACCTGGACAT TCCCCAGGAT GCCCTGCTGT TCAGCATCAC TCAAAAGCCA 
CGCCATGGCC TCCTCATCGA TAGGGGGTTT AGCAAAGACT TCTCTGAGAA TAAGCAGCCA 
GCCAACCCTC ACCAGAAACA TGCACCTGTT CACAGCTTTT CCATGGAACT CCTCAAGACT 
GGAATGAGGT TGACGTACAT GCATGATGAC TCAGAGAGCC TTGCTGATGA TTTTACAATC 
CAATTGTCAG ATGGGAAACA TAAGATACTT AAAACCATTT CAGTAGAGGT CATCCCAGTT 
AATGATGAAA AACCAATGCT GAGCAAGAAG GCTGAAATTG CAATGAATAT GGGTGAAACT 
CGTATTATTT CCAGTGCTAT TCTTTCAGCC ATAGATGAAG ACTCACCCAG GGAGAAGATT 
TACTATGTAT TTGAAAGGCT TCCCCAAAAT GGGCAACTTC AGCTTAAGAT AGGGAGGGAC 
TGGGTTCCTC TCTCCCCTGG CATGAAATGC ACTCAGGAGG AAGTGGATCT GAACTTGCTG 
AGATACACAC ACACCGGGGC AATGGATTCC CAGAATCAAG ATAGCTTCAC CTTCTACCTT 
TGGGATGGCA ACAACAGGTC CCCTGCTCTT GACTGTCAAA TCACCATCAA GGACATGGAA 
AAAGGTGATA TTGTCATCCT TACAAAACCA CTCGTGGTGT CTAAAGGTGA TAGAGGTTTC 
TTAACAACCA CCACGCTCCT GGCTGTGGAC GGAACAGACA AGCCTGAGGA ACTGCTCTAT 
GTCATCACCT CCCCTCCGCG ATATGGCCAG ATCGAATATG TTCACTATCC TGGAGTTCCC 
ATTACAAACT TCAGCCAAAT GGATGTAGTG GGGCAGACAG TTTGCTATGT ACACAAGAGC 
AAGGTGACTG TCTCCAGTGA CAGATTCAGA TTCATCATCA GCAATGGACT GCGGACCGAG 
CACGGGGTGT TTGAGATCAC ACTGGAGACT GTGGACAGAG CCCTGCCTGT GGTAACCAGG 
AACAAGGGGT TGAGACTGGC CCAAGGGGCC GTGGGCCTGC TTTCCCCTGA CCTCCTTCAG 
CTGACCGACC CTGATACACC TGCGGAGAAC CTCACCTTCC TCTTGGTTCA GCTCCCCCAG 
CATGGCCAGC TCTACCTGTG GGGGACAGGG CTACTTCAAC ACAATTTCAC CCAGCAGGAT 
GTGGACAGCA AGAATGTGGC CTATCGGCAC TCAGGAGGGG ACTCCCAGAC TGACTGCTTT 
ACTTTCATGG CCACAGATGG GACAAACCAA GGCTTTATTG TGAATGGGAG AGTGTGGGAA 
GAACCTGTTT TATTCACCAT TCAGGTAGAC CAATTGGACA AAACAGCTCC TCGTATCACA 
CTCTTGCATT CCCCTTCTCA AGTGGGGCTC CTGAAAAATG GCTGCTACGG GATTTACATC 
ACTTCCCGCG TGTTGAAGGC ATCAGACCCT GACACTGAGG ACGATCAGAT CATCTTTAAA 
ATTCTACAAG GCCCAAAACA TGGACATCTG GAGAACACAA CAACAGGTGA ATTTATCCAT 
GAGAAATTTA GCCAAAAGGA CTTAAACAGT AAGACTATTC TTTACATCAT AAACCCATCT 
TTGGAAGTAA ATTCAGATAC CGTGGAATTT CAAATCATGG ACCCCACAGG GAACTCGGCC 
ACTCCTCAAA TTTTGGAACT GAAGTGGTCT CATATTGAAT GGTCACAGAC CGAATATGAA 
GTCTGTGAGA ATGTGGGTTT GTTGCCCTTG GAAATTATCA GAAGGGGATA TTCCATGGAC 
TCGGCCTTTG TGGGTATAAA GGTCAACCAA GTGTCAGCTG CAGTTGGAAA AGATTTCACC 
GTGATTCCAT CTAAACTGAT TCAGTTTGAC CCAGGACAAT GCCATCCTTC ATATTCCTCC 
AACCAAAGCA AGCACAGCAC ATGGGAGAAG GGCATTTGGC ATCTGCTGCC CCCAGGGTCT 
TCCTCATCCA CCACTTCTGG TTCCTTTCAT CTGGAAAGAA GACCTCTTCC ATCTTCCATG 
CAGCTAGCAG TCATCAGGGG AGACACCCTG CGGGGCTTTG ATTCTACAGA TCTTTCTCAA 
AGGAAGCTTA GGACCCGTGG GAATGGCAAA ACAGTTCGTC CATCCTCTGT TTATAGAAAT 
GGAACAGACA TCATCTATAA TTATCATGGG ATAGTTTCCT TGAAACTGGA GGATGACAGT 
TTCCCAACTC ACAAAAGGAA GGCCAAAGTA TCCATCATTA GTCAGCCACA AAAGACAATC 
AAAGTGGCAG AACTGCCTCA AGCAGATAAG GTGGAATCCA CAACTGACTC ACACTTCCCC 
AGACAGGACC AGTTGCCCTC ATTTCCAAAG AACTGCACTC TGGAATTAAA GGGACTCTTC 
CATTTTGAAG AAGGCATCCA GAAGCTGTAT CAGTGCAATG GGATCGCCTG GAAAGCCTGG 
AGTCCCCAAA CCAAGGATGT GGAAGACAAA TCCTGTCCAG CCGGGTGGCA CCAGCACTCA 
GGCTACTGTC ACATCTTGAT CACAGAGCAG AAAGGCACCT GGAATGCGGC TGCCCAAGCT 
TGCAGGGAAC AATACCTGGG CAACCTTGTA ACTGTATTCT CCAGGCAGCA CATGCGGTGG 
CTCTGGGACA TTGGTGGGAG AAAGTCCTTT TGGATAGGTT TGAACGACCA AGTGCATGCT 
GGCCACTGGG AGTGGATCGG TGGTGAACCT GTTGCCTTCA CCAATGGGAG AAGAGGGCCC 
TCTCAACGCT CCAAGCTTGG AAAGAGCTGT GTTTTGGTTC AAAGACAAGG GAAATGGCAA 
ACAAAAGACT GTAGGAGAGC CAAACCTCAT AATTATGTGT GTTCCAGAAA ACTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FREM1 ( XM_017014325.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016869814.1 Homo sapiens FRAS1 related extracellular matrix 1 (FREM1), transcript variant X11, mRNA.

Price & Availability↑

CloneID	OHu98461
Clone ID Related Accession (Same CDS sequence)	XM_017014325.1 , XM_017014325.2
Accession Version	XM_017014325.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6300bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	FRAS1-related extracellular matrix protein 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACTCTC TGAGTTGGGG GGCTGCGAAT GCCGTGCTGC TGCTGCTCCT CCTGGCCTGG 
GCCAGCCCCA CCTTCATCAG CATCAACCGC GGGGTGAGGG TGATGAAGGG CCACTCTGCC 
TTCCTGTCAG GAGATGACCT GAAGTTTGCC ATCCCTAAAG AGAAAGATGC CTGCAAAGTG 
GAAGTTGTGA TGAATGAGCC AATAACCCAG AGGGTTGGGA AACTCACTCC ACAGGTCTTT 
GACTGCCATT TCCTTCCCAA CGAAGTCAAG TATGTTCACA ATGGTTGTCC AATTCTTGAT 
GAAGACACAG TGAAGCTCAG ACTTTACAGA TTTACTGAAA GAGATACCTT CATAGAAACT 
TTTATCCTGT GGGTCTATCT CCTGGAACCA GACTGTAACA TCATCCATAT GAGTAACAAT 
GTGCTGGAGG TGCCTGAATT CAATGGCTTG TCCCAAGCGA TTGATAAAAA TCTGCTCAGA 
TTCGATTATG ATAGGATGGC TAGCCTGGAA TGTACCGTCA GCCTGGACAC TGCGAGAACT 
CGGCTGCCAG CCCATGGCCA GATGGTTCTC GGGGAGCCTC GACCAGAAGA ACCTCGTGGA 
GATCAGCCAC ACAGTTTTTT CCCAGAGTCC CGTATGATGT CTTTTAATGC CTTTCTGGAA 
CTGAGAGCAA AACTGAAGTG TCCAGGTGGG AGCTGTACCC CAGGATTAAA GAAAATAGGA 
AGCCTCAAAG TGAGCTGTGA GGAGTTCCTG CTGATGGGCC TTCGTTATCA GCATCTGGAT 
CCCCCTTCAC CCAACATTGA TTATATCTCC ATCCAACTGG ACCTCACAGA TACCAGGAGC 
AAAATTGTGT ACAAGTCAGA GAGTGCGTGG CTGCCTGTCT ATATCAGAGC TGGAATTCCG 
AATCAGATTC CAAAGGCTGC ATTCATGGCC GTGTTTATTC TGGAAGTGGA TCAGTTCATC 
CTGACCTCCT TGACTACATC AGTTCTGGAC TGTGAAGAAG ATGAGACCCC TAAACCCTTG 
CTGGTGTTCA ACATTACTAA AGCCCCGCTC CAGGGCTATG TGACTCACCT GTTGGATCAC 
ACCAGACCAA TCTCCTCATT CACCTGGAAA GATCTCAGTG ACATGCAGAT CGCCTATCAG 
CCACCAAACA GCAGCCATTC TGAGAGGAGA CATGATGAGG TAGAATTGGA GGTATACGAC 
TTCTTCTTTG AAAGGAGTGC ACCTATGACA GTCCACATCT CCATCAGAAC AGCAGATACA 
AATGCCCCCC GTGTATCCTG GAATACAGGT CTGAGTCTCC TTGAGGGGCA GTCTCGAGCC 
ATCACTTGGG AACAGTTTCA GGTTGTCGAC AATGACGACA TTGGTGCTGT CCGGCTAGTC 
ACCGTTGGTG GCCTGCAGCA TGGATGGCTG ACTTTAAGAG GGGGGAAAGG GTTTCTCTTC 
ACCGTGGCTG ACCTCCAGGC TGGAGTTGTT CGCTATCATC ATGATGACAG CGACTCCACC 
AAAGACTTCG TGGTCTTCCG GATATTTGAT GGCCATCACA GCATCCGTCA CAAATTCCCC 
ATCAACGTCT TGCCCAAAGA TGATAGTCCC CCGTTCCTCA TAACCAATGT TGTGATTGAA 
CTGGAGGAGG GGCAGACCAT CCTGATCCAG GGATCCATGC TGCGAGCTTC AGATGTGGAC 
GCCAGTGATG ACTACATCTT CTTCAATATC ACAAAGCCTC CACAGGCTGG GGAGATCATG 
AAGAAGCCAG GGCCAGGACT GATAGGCTAT CCTGTCCATG GCTTCCTTCA GAGGGATTTG 
TTTAATGGAA TCATTTATTA TCGTCATTTT GGTGGAGAAA TCTTTGAAGA TTCTTTTCAA 
TTTGTCCTGT GGGACAGCCA TGAACCTCCA AATCTTTCAG TGCCACAGGT GGCAACAATC 
CATATAACTC CAGTGGATGA CCAGCTTCCA AAAGAGGCTC CTGGAGTTTC TCGGCATTTG 
GTTGTCAAGG AAACTGAGGT GGCCTATATA ACTAAGAAAC AGCTACATTT TATAGATTCA 
GAATCATATG ACAGGGAGCT GGTCTACACA ATAACTACTC CTCCATTTTT CTCCTTCAGC 
CACAGACACT TGGATGCTGG GAAATTATTT ATGGTGGACA GCATACCAAA AGTAGTTAAG 
AATCCTACGG CCCTGGAGCT GAGGTCATTC ACTCAGCATG CTGTGAACTA TATGAAAGTG 
GCCTACATGC CCCCCATGCA AGACATTGGT CCCCATTGCA GAGATGTCCA GTTCACATTT 
TCTGTCAGTA ACCAACATGG CGGTACTTTG CATGGGATCT GCTTTAACAT TACAATCCTC 
CCAGTGGACA ATCAAGTTCC TGAGGCGTTC ACCAACCCTC TGAAAGTGAC TGAGGGAGGT 
CAAAGCATCA TCAGCACAGA GCACATTCTA ATTTCTGATG CAGATACCAA GCTGGACAAT 
ATTGACCTCT CCCTGCGGGA ATTGCCTCTG CACGGAAGGG TGGAGCTGAA TGGATTTCCT 
CTAAATTCAG GGGGCACATT TTCTTGGGGC GATCTCCATA CCTTAAAAGT TAGGTATCAA 
CATGATGGAA CTGAAGTTCT TCAGGATGAC CTACTCTTGG AGGTCACCGA TGGCACAAAT 
TCAGCAGAAT TTGTACTACA TGTTGAGGTA TTCCCTGTCA ACGATGAGCC ACCAGTCTTA 
AAGGCTGACC TCATGCCTGT CATGAATTGC TCAGAGGGAG GAGAGGTGGT CATCACCTCT 
GAATACATTT TTGCTACTGA TGTGGACAGC GATAACTTGA AGTTGATGTT TGTGATTGCT 
CGCGAACCTC AGCATGGGGT GGTGAGGAGA GCTGGAGTCA CAGTGGATCA GTTCTCTCAG 
AGAGATGTTA TCTCAGAGGC CGTGACATAC AAACACACAG GTGGCGAGAT TGGCCTGATG 
CCTTGTTTTG ACACCATTAC ATTGGTGGTT TCGGATGGAG AGGCTGGCCC TTTTGTGAAT 
GGCTGTTGCT ACAATGGACC CAATCCATCT GTTCCACTTC ATGCGTCCTT TCCAGTGTAT 
GATCTCAACA TCACGGTATA CCCAGTAGAC AACCAGCCAC CTTCCATTGC AATAGGTCCC 
GTGTTTGTTG TAGATGAGGG CTGCTCAACA GCCCTTACTG TTAACCATTT GTCCGCCACT 
GACCCTGACA CTGCAGCAGA TGACTTGGAA TTTGTTTTGG TTTCTCCTCC TCAGTTTGGC 
TACCTCGAAA ATATACTCCC TTCTGTGGGT TTTGAAAAAA GCAATATTGG CATAAGTATA 
GATTCATTTC AGTGGAAAGA CATGAACGCT TTTCACATTA ACTATGTGCA GTCCAGGCAT 
CTGAGGATAG AACCAACTGC CGACCAGTTC ACGGTGTACG TCACAGATGG GAAGCATCAC 
TCCTTGGAGA TACCATTTTC TATTATAATC AACCCCACAA ATGATGAAGC TCCTGACTTT 
GTAGTGCAGA ATATTACTGT GTGTGAGGGT CAGATGAAAG AGCTGGACTC TTCCATCATC 
AGCGCTGTGG ACCTGGACAT TCCCCAGGAT GCCCTGCTGT TCAGCATCAC TCAAAAGCCA 
CGCCATGGCC TCCTCATCGA TAGGGGGTTT AGCAAAGACT TCTCTGAGAA TAAGCAGCCA 
GCCAACCCTC ACCAGAAACA TGCACCTGTT CACAGCTTTT CCATGGAACT CCTCAAGACT 
GGAATGAGGT TGACGTACAT GCATGATGAC TCAGAGAGCC TTGCTGATGA TTTTACAATC 
CAATTGTCAG ATGGGAAACA TAAGATACTT AAAACCATTT CAGTAGAGGT CATCCCAGTT 
AATGATGAAA AACCAATGCT GAGCAAGAAG GCTGAAATTG CAATGAATAT GGGTGAAACT 
CGTATTATTT CCAGTGCTAT TCTTTCAGCC ATAGATGAAG ACTCACCCAG GGAGAAGATT 
TACTATGTAT TTGAAAGGCT TCCCCAAAAT GGGCAACTTC AGCTTAAGAT AGGGAGGGAC 
TGGGTTCCTC TCTCCCCTGG CATGAAATGC ACTCAGGAGG AAGTGGATCT GAACTTGCTG 
AGATACACAC ACACCGGGGC AATGGATTCC CAGAATCAAG ATAGCTTCAC CTTCTACCTT 
TGGGATGGCA ACAACAGGTC CCCTGCTCTT GACTGTCAAA TCACCATCAA GGACATGGAA 
AAAGGTGATA TTGTCATCCT TACAAAACCA CTCGTGGTGT CTAAAGGTGA TAGAGGTTTC 
TTAACAACCA CCACGCTCCT GGCTGTGGAC GGAACAGACA AGCCTGAGGA ACTGCTCTAT 
GTCATCACCT CCCCTCCGCG ATATGGCCAG ATCGAATATG TTCACTATCC TGGAGTTCCC 
ATTACAAACT TCAGCCAAAT GGATGTAGTG GGGCAGACAG TTTGCTATGT ACACAAGAGC 
AAGGTGACTG TCTCCAGTGA CAGATTCAGA TTCATCATCA GCAATGGACT GCGGACCGAG 
CACGGGGTGT TTGAGATCAC ACTGGAGACT GTGGACAGAG CCCTGCCTGT GGTAACCAGG 
AACAAGGGGT TGAGACTGGC CCAAGGGGCC GTGGGCCTGC TTTCCCCTGA CCTCCTTCAG 
CTGACCGACC CTGATACACC TGCGGAGAAC CTCACCTTCC TCTTGGTTCA GCTCCCCCAG 
CATGGCCAGC TCTACCTGTG GGGGACAGGG CTACTTCAAC ACAATTTCAC CCAGCAGGAT 
GTGGACAGCA AGAATGTGGC CTATCGGCAC TCAGGAGGGG ACTCCCAGAC TGACTGCTTT 
ACTTTCATGG CCACAGATGG GACAAACCAA GGCTTTATTG TGAATGGGAG AGTGTGGGAA 
GAACCTGTTT TATTCACCAT TCAGGTAGAC CAATTGGACA AAACAGCTCC TCGTATCACA 
CTCTTGCATT CCCCTTCTCA AGTGGGGCTC CTGAAAAATG GCTGCTACGG GATTTACATC 
ACTTCCCGCG TGTTGAAGGC ATCAGACCCT GACACTGAGG ACGATCAGAT CATCTTTAAA 
ATTCTACAAG GCCCAAAACA TGGACATCTG GAGAACACAA CAACAGGTGA ATTTATCCAT 
GAGAAATTTA GCCAAAAGGA CTTAAACAGT AAGACTATTC TTTACATCAT AAACCCATCT 
TTGGAAGTAA ATTCAGATAC CGTGGAATTT CAAATCATGG ACCCCACAGG GAACTCGGCC 
ACTCCTCAAA TTTTGGAACT GAAGTGGTCT CATATTGAAT GGTCACAGAC CGAATATGAA 
GTCTGTGAGA ATGTGGGTTT GTTGCCCTTG GAAATTATCA GAAGGGGATA TTCCATGGAC 
TCGGCCTTTG TGGGTATAAA GGTCAACCAA GTGTCAGCTG CAGTTGGAAA AGATTTCACC 
GTGATTCCAT CTAAACTGAT TCAGTTTGAC CCAGGAATGT CAACTAAGAT GTGGAATATA 
GCAATTACCT ATGACGGATT AGAGGAAGAT GATGAGGTCT TTGAAGTAAT TCTGAACTCC 
CCTGTGAATG CAGTTCTTGG CACAAAGACA AAAGCTGCAG TGAAAATTTT GGACTCAAAA 
GGAGGACAAT GCCATCCTTC ATATTCCTCC AACCAAAGCA AGCACAGCAC ATGGGAGAAG 
GGCATTTGGC ATCTGCTGCC CCCAGGGTCT TCCTCATCCA CCACTTCTGG TTCCTTTCAT 
CTGGAAAGAA GACCTCTTCC ATCTTCCATG CAGCTAGCAG TCATCAGGGG AGACACCCTG 
CGGGGCTTTG ATTCTACAGA TCTTTCTCAA AGGAAGCTTA GGACCCGTGG GAATGGCAAA 
ACAGTTCGTC CATCCTCTGT TTATAGAAAT GGAACAGACA TCATCTATAA TTATCATGGG 
ATAGTTTCCT TGAAACTGGA GGATGACAGT TTCCCAACTC ACAAAAGGAA GGCCAAAGTA 
TCCATCATTA GTCAGCCACA AAAGACAATC AAAGTGGCAG AACTGCCTCA AGCAGATAAG 
GTGGAATCCA CAACTGACTC ACACTTCCCC AGACAGGACC AGTTGCCCTC ATTTCCAAAG 
AACTGCACTC TGGAATTAAA GGGACTCTTC CATTTTGAAG AAGGCATCCA GAAGCTGTAT 
CAGTGCAATG GGATCGCCTG GAAAGCCTGG AGTCCCCAAA CCAAGATACC TGGGCAACCT 
TGTAACTGTA TTCTCCAGGC AGCACATGCG GTGGCTCTGG GACATTGGTG GGAGAAAGTC 
CTTTTGGATA GGTTTGAACG ACCAAGTGCA TGCTGGCCAC TGGGAGTGGA TCGGTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016869814.1
CDS	1065..7364
Translation	MNSLSWGAANAVLLLLLLAWASPTFISINRGVRVMKGHSAFLSGDDLKFAIPKEKDACKVEVVMNEPITQRVGKLTPQVFDCHFLPNEVKYVHNGCPILDEDTVKLRLYRFTERDTFIETFILWVYLLEPDCNIIHMSNNVLEVPEFNGLSQAIDKNLLRFDYDRMASLECTVSLDTARTRLPAHGQMVLGEPRPEEPRGDQPHSFFPESRMMSFNAFLELRAKLKCPGGSCTPGLKKIGSLKVSCEEFLLMGLRYQHLDPPSPNIDYISIQLDLTDTRSKIVYKSESAWLPVYIRAGIPNQIPKAAFMAVFILEVDQFILTSLTTSVLDCEEDETPKPLLVFNITKAPLQGYVTHLLDHTRPISSFTWKDLSDMQIAYQPPNSSHSERRHDEVELEVYDFFFERSAPMTVHISIRTADTNAPRVSWNTGLSLLEGQSRAITWEQFQVVDNDDIGAVRLVTVGGLQHGWLTLRGGKGFLFTVADLQAGVVRYHHDDSDSTKDFVVFRIFDGHHSIRHKFPINVLPKDDSPPFLITNVVIELEEGQTILIQGSMLRASDVDASDDYIFFNITKPPQAGEIMKKPGPGLIGYPVHGFLQRDLFNGIIYYRHFGGEIFEDSFQFVLWDSHEPPNLSVPQVATIHITPVDDQLPKEAPGVSRHLVVKETEVAYITKKQLHFIDSESYDRELVYTITTPPFFSFSHRHLDAGKLFMVDSIPKVVKNPTALELRSFTQHAVNYMKVAYMPPMQDIGPHCRDVQFTFSVSNQHGGTLHGICFNITILPVDNQVPEAFTNPLKVTEGGQSIISTEHILISDADTKLDNIDLSLRELPLHGRVELNGFPLNSGGTFSWGDLHTLKVRYQHDGTEVLQDDLLLEVTDGTNSAEFVLHVEVFPVNDEPPVLKADLMPVMNCSEGGEVVITSEYIFATDVDSDNLKLMFVIAREPQHGVVRRAGVTVDQFSQRDVISEAVTYKHTGGEIGLMPCFDTITLVVSDGEAGPFVNGCCYNGPNPSVPLHASFPVYDLNITVYPVDNQPPSIAIGPVFVVDEGCSTALTVNHLSATDPDTAADDLEFVLVSPPQFGYLENILPSVGFEKSNIGISIDSFQWKDMNAFHINYVQSRHLRIEPTADQFTVYVTDGKHHSLEIPFSIIINPTNDEAPDFVVQNITVCEGQMKELDSSIISAVDLDIPQDALLFSITQKPRHGLLIDRGFSKDFSENKQPANPHQKHAPVHSFSMELLKTGMRLTYMHDDSESLADDFTIQLSDGKHKILKTISVEVIPVNDEKPMLSKKAEIAMNMGETRIISSAILSAIDEDSPREKIYYVFERLPQNGQLQLKIGRDWVPLSPGMKCTQEEVDLNLLRYTHTGAMDSQNQDSFTFYLWDGNNRSPALDCQITIKDMEKGDIVILTKPLVVSKGDRGFLTTTTLLAVDGTDKPEELLYVITSPPRYGQIEYVHYPGVPITNFSQMDVVGQTVCYVHKSKVTVSSDRFRFIISNGLRTEHGVFEITLETVDRALPVVTRNKGLRLAQGAVGLLSPDLLQLTDPDTPAENLTFLLVQLPQHGQLYLWGTGLLQHNFTQQDVDSKNVAYRHSGGDSQTDCFTFMATDGTNQGFIVNGRVWEEPVLFTIQVDQLDKTAPRITLLHSPSQVGLLKNGCYGIYITSRVLKASDPDTEDDQIIFKILQGPKHGHLENTTTGEFIHEKFSQKDLNSKTILYIINPSLEVNSDTVEFQIMDPTGNSATPQILELKWSHIEWSQTEYEVCENVGLLPLEIIRRGYSMDSAFVGIKVNQVSAAVGKDFTVIPSKLIQFDPGMSTKMWNIAITYDGLEEDDEVFEVILNSPVNAVLGTKTKAAVKILDSKGGQCHPSYSSNQSKHSTWEKGIWHLLPPGSSSSTTSGSFHLERRPLPSSMQLAVIRGDTLRGFDSTDLSQRKLRTRGNGKTVRPSSVYRNGTDIIYNYHGIVSLKLEDDSFPTHKRKAKVSIISQPQKTIKVAELPQADKVESTTDSHFPRQDQLPSFPKNCTLELKGLFHFEEGIQKLYQCNGIAWKAWSPQTKIPGQPCNCILQAAHAVALGHWWEKVLLDRFERPSACWPLGVDRW

Target ORF information:

RefSeq Version	XM_017014325.1
Organism	Homo sapiens(human)
Definition	Homo sapiens FRAS1 related extracellular matrix 1 (FREM1), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017014325.1

ORF Insert Sequence:

ATGAACTCTC TGAGTTGGGG GGCTGCGAAT GCCGTGCTGC TGCTGCTCCT CCTGGCCTGG 
GCCAGCCCCA CCTTCATCAG CATCAACCGC GGGGTGAGGG TGATGAAGGG CCACTCTGCC 
TTCCTGTCAG GAGATGACCT GAAGTTTGCC ATCCCTAAAG AGAAAGATGC CTGCAAAGTG 
GAAGTTGTGA TGAATGAGCC AATAACCCAG AGGGTTGGGA AACTCACTCC ACAGGTCTTT 
GACTGCCATT TCCTTCCCAA CGAAGTCAAG TATGTTCACA ATGGTTGTCC AATTCTTGAT 
GAAGACACAG TGAAGCTCAG ACTTTACAGA TTTACTGAAA GAGATACCTT CATAGAAACT 
TTTATCCTGT GGGTCTATCT CCTGGAACCA GACTGTAACA TCATCCATAT GAGTAACAAT 
GTGCTGGAGG TGCCTGAATT CAATGGCTTG TCCCAAGCGA TTGATAAAAA TCTGCTCAGA 
TTCGATTATG ATAGGATGGC TAGCCTGGAA TGTACCGTCA GCCTGGACAC TGCGAGAACT 
CGGCTGCCAG CCCATGGCCA GATGGTTCTC GGGGAGCCTC GACCAGAAGA ACCTCGTGGA 
GATCAGCCAC ACAGTTTTTT CCCAGAGTCC CGTATGATGT CTTTTAATGC CTTTCTGGAA 
CTGAGAGCAA AACTGAAGTG TCCAGGTGGG AGCTGTACCC CAGGATTAAA GAAAATAGGA 
AGCCTCAAAG TGAGCTGTGA GGAGTTCCTG CTGATGGGCC TTCGTTATCA GCATCTGGAT 
CCCCCTTCAC CCAACATTGA TTATATCTCC ATCCAACTGG ACCTCACAGA TACCAGGAGC 
AAAATTGTGT ACAAGTCAGA GAGTGCGTGG CTGCCTGTCT ATATCAGAGC TGGAATTCCG 
AATCAGATTC CAAAGGCTGC ATTCATGGCC GTGTTTATTC TGGAAGTGGA TCAGTTCATC 
CTGACCTCCT TGACTACATC AGTTCTGGAC TGTGAAGAAG ATGAGACCCC TAAACCCTTG 
CTGGTGTTCA ACATTACTAA AGCCCCGCTC CAGGGCTATG TGACTCACCT GTTGGATCAC 
ACCAGACCAA TCTCCTCATT CACCTGGAAA GATCTCAGTG ACATGCAGAT CGCCTATCAG 
CCACCAAACA GCAGCCATTC TGAGAGGAGA CATGATGAGG TAGAATTGGA GGTATACGAC 
TTCTTCTTTG AAAGGAGTGC ACCTATGACA GTCCACATCT CCATCAGAAC AGCAGATACA 
AATGCCCCCC GTGTATCCTG GAATACAGGT CTGAGTCTCC TTGAGGGGCA GTCTCGAGCC 
ATCACTTGGG AACAGTTTCA GGTTGTCGAC AATGACGACA TTGGTGCTGT CCGGCTAGTC 
ACCGTTGGTG GCCTGCAGCA TGGATGGCTG ACTTTAAGAG GGGGGAAAGG GTTTCTCTTC 
ACCGTGGCTG ACCTCCAGGC TGGAGTTGTT CGCTATCATC ATGATGACAG CGACTCCACC 
AAAGACTTCG TGGTCTTCCG GATATTTGAT GGCCATCACA GCATCCGTCA CAAATTCCCC 
ATCAACGTCT TGCCCAAAGA TGATAGTCCC CCGTTCCTCA TAACCAATGT TGTGATTGAA 
CTGGAGGAGG GGCAGACCAT CCTGATCCAG GGATCCATGC TGCGAGCTTC AGATGTGGAC 
GCCAGTGATG ACTACATCTT CTTCAATATC ACAAAGCCTC CACAGGCTGG GGAGATCATG 
AAGAAGCCAG GGCCAGGACT GATAGGCTAT CCTGTCCATG GCTTCCTTCA GAGGGATTTG 
TTTAATGGAA TCATTTATTA TCGTCATTTT GGTGGAGAAA TCTTTGAAGA TTCTTTTCAA 
TTTGTCCTGT GGGACAGCCA TGAACCTCCA AATCTTTCAG TGCCACAGGT GGCAACAATC 
CATATAACTC CAGTGGATGA CCAGCTTCCA AAAGAGGCTC CTGGAGTTTC TCGGCATTTG 
GTTGTCAAGG AAACTGAGGT GGCCTATATA ACTAAGAAAC AGCTACATTT TATAGATTCA 
GAATCATATG ACAGGGAGCT GGTCTACACA ATAACTACTC CTCCATTTTT CTCCTTCAGC 
CACAGACACT TGGATGCTGG GAAATTATTT ATGGTGGACA GCATACCAAA AGTAGTTAAG 
AATCCTACGG CCCTGGAGCT GAGGTCATTC ACTCAGCATG CTGTGAACTA TATGAAAGTG 
GCCTACATGC CCCCCATGCA AGACATTGGT CCCCATTGCA GAGATGTCCA GTTCACATTT 
TCTGTCAGTA ACCAACATGG CGGTACTTTG CATGGGATCT GCTTTAACAT TACAATCCTC 
CCAGTGGACA ATCAAGTTCC TGAGGCGTTC ACCAACCCTC TGAAAGTGAC TGAGGGAGGT 
CAAAGCATCA TCAGCACAGA GCACATTCTA ATTTCTGATG CAGATACCAA GCTGGACAAT 
ATTGACCTCT CCCTGCGGGA ATTGCCTCTG CACGGAAGGG TGGAGCTGAA TGGATTTCCT 
CTAAATTCAG GGGGCACATT TTCTTGGGGC GATCTCCATA CCTTAAAAGT TAGGTATCAA 
CATGATGGAA CTGAAGTTCT TCAGGATGAC CTACTCTTGG AGGTCACCGA TGGCACAAAT 
TCAGCAGAAT TTGTACTACA TGTTGAGGTA TTCCCTGTCA ACGATGAGCC ACCAGTCTTA 
AAGGCTGACC TCATGCCTGT CATGAATTGC TCAGAGGGAG GAGAGGTGGT CATCACCTCT 
GAATACATTT TTGCTACTGA TGTGGACAGC GATAACTTGA AGTTGATGTT TGTGATTGCT 
CGCGAACCTC AGCATGGGGT GGTGAGGAGA GCTGGAGTCA CAGTGGATCA GTTCTCTCAG 
AGAGATGTTA TCTCAGAGGC CGTGACATAC AAACACACAG GTGGCGAGAT TGGCCTGATG 
CCTTGTTTTG ACACCATTAC ATTGGTGGTT TCGGATGGAG AGGCTGGCCC TTTTGTGAAT 
GGCTGTTGCT ACAATGGACC CAATCCATCT GTTCCACTTC ATGCGTCCTT TCCAGTGTAT 
GATCTCAACA TCACGGTATA CCCAGTAGAC AACCAGCCAC CTTCCATTGC AATAGGTCCC 
GTGTTTGTTG TAGATGAGGG CTGCTCAACA GCCCTTACTG TTAACCATTT GTCCGCCACT 
GACCCTGACA CTGCAGCAGA TGACTTGGAA TTTGTTTTGG TTTCTCCTCC TCAGTTTGGC 
TACCTCGAAA ATATACTCCC TTCTGTGGGT TTTGAAAAAA GCAATATTGG CATAAGTATA 
GATTCATTTC AGTGGAAAGA CATGAACGCT TTTCACATTA ACTATGTGCA GTCCAGGCAT 
CTGAGGATAG AACCAACTGC CGACCAGTTC ACGGTGTACG TCACAGATGG GAAGCATCAC 
TCCTTGGAGA TACCATTTTC TATTATAATC AACCCCACAA ATGATGAAGC TCCTGACTTT 
GTAGTGCAGA ATATTACTGT GTGTGAGGGT CAGATGAAAG AGCTGGACTC TTCCATCATC 
AGCGCTGTGG ACCTGGACAT TCCCCAGGAT GCCCTGCTGT TCAGCATCAC TCAAAAGCCA 
CGCCATGGCC TCCTCATCGA TAGGGGGTTT AGCAAAGACT TCTCTGAGAA TAAGCAGCCA 
GCCAACCCTC ACCAGAAACA TGCACCTGTT CACAGCTTTT CCATGGAACT CCTCAAGACT 
GGAATGAGGT TGACGTACAT GCATGATGAC TCAGAGAGCC TTGCTGATGA TTTTACAATC 
CAATTGTCAG ATGGGAAACA TAAGATACTT AAAACCATTT CAGTAGAGGT CATCCCAGTT 
AATGATGAAA AACCAATGCT GAGCAAGAAG GCTGAAATTG CAATGAATAT GGGTGAAACT 
CGTATTATTT CCAGTGCTAT TCTTTCAGCC ATAGATGAAG ACTCACCCAG GGAGAAGATT 
TACTATGTAT TTGAAAGGCT TCCCCAAAAT GGGCAACTTC AGCTTAAGAT AGGGAGGGAC 
TGGGTTCCTC TCTCCCCTGG CATGAAATGC ACTCAGGAGG AAGTGGATCT GAACTTGCTG 
AGATACACAC ACACCGGGGC AATGGATTCC CAGAATCAAG ATAGCTTCAC CTTCTACCTT 
TGGGATGGCA ACAACAGGTC CCCTGCTCTT GACTGTCAAA TCACCATCAA GGACATGGAA 
AAAGGTGATA TTGTCATCCT TACAAAACCA CTCGTGGTGT CTAAAGGTGA TAGAGGTTTC 
TTAACAACCA CCACGCTCCT GGCTGTGGAC GGAACAGACA AGCCTGAGGA ACTGCTCTAT 
GTCATCACCT CCCCTCCGCG ATATGGCCAG ATCGAATATG TTCACTATCC TGGAGTTCCC 
ATTACAAACT TCAGCCAAAT GGATGTAGTG GGGCAGACAG TTTGCTATGT ACACAAGAGC 
AAGGTGACTG TCTCCAGTGA CAGATTCAGA TTCATCATCA GCAATGGACT GCGGACCGAG 
CACGGGGTGT TTGAGATCAC ACTGGAGACT GTGGACAGAG CCCTGCCTGT GGTAACCAGG 
AACAAGGGGT TGAGACTGGC CCAAGGGGCC GTGGGCCTGC TTTCCCCTGA CCTCCTTCAG 
CTGACCGACC CTGATACACC TGCGGAGAAC CTCACCTTCC TCTTGGTTCA GCTCCCCCAG 
CATGGCCAGC TCTACCTGTG GGGGACAGGG CTACTTCAAC ACAATTTCAC CCAGCAGGAT 
GTGGACAGCA AGAATGTGGC CTATCGGCAC TCAGGAGGGG ACTCCCAGAC TGACTGCTTT 
ACTTTCATGG CCACAGATGG GACAAACCAA GGCTTTATTG TGAATGGGAG AGTGTGGGAA 
GAACCTGTTT TATTCACCAT TCAGGTAGAC CAATTGGACA AAACAGCTCC TCGTATCACA 
CTCTTGCATT CCCCTTCTCA AGTGGGGCTC CTGAAAAATG GCTGCTACGG GATTTACATC 
ACTTCCCGCG TGTTGAAGGC ATCAGACCCT GACACTGAGG ACGATCAGAT CATCTTTAAA 
ATTCTACAAG GCCCAAAACA TGGACATCTG GAGAACACAA CAACAGGTGA ATTTATCCAT 
GAGAAATTTA GCCAAAAGGA CTTAAACAGT AAGACTATTC TTTACATCAT AAACCCATCT 
TTGGAAGTAA ATTCAGATAC CGTGGAATTT CAAATCATGG ACCCCACAGG GAACTCGGCC 
ACTCCTCAAA TTTTGGAACT GAAGTGGTCT CATATTGAAT GGTCACAGAC CGAATATGAA 
GTCTGTGAGA ATGTGGGTTT GTTGCCCTTG GAAATTATCA GAAGGGGATA TTCCATGGAC 
TCGGCCTTTG TGGGTATAAA GGTCAACCAA GTGTCAGCTG CAGTTGGAAA AGATTTCACC 
GTGATTCCAT CTAAACTGAT TCAGTTTGAC CCAGGAATGT CAACTAAGAT GTGGAATATA 
GCAATTACCT ATGACGGATT AGAGGAAGAT GATGAGGTCT TTGAAGTAAT TCTGAACTCC 
CCTGTGAATG CAGTTCTTGG CACAAAGACA AAAGCTGCAG TGAAAATTTT GGACTCAAAA 
GGAGGACAAT GCCATCCTTC ATATTCCTCC AACCAAAGCA AGCACAGCAC ATGGGAGAAG 
GGCATTTGGC ATCTGCTGCC CCCAGGGTCT TCCTCATCCA CCACTTCTGG TTCCTTTCAT 
CTGGAAAGAA GACCTCTTCC ATCTTCCATG CAGCTAGCAG TCATCAGGGG AGACACCCTG 
CGGGGCTTTG ATTCTACAGA TCTTTCTCAA AGGAAGCTTA GGACCCGTGG GAATGGCAAA 
ACAGTTCGTC CATCCTCTGT TTATAGAAAT GGAACAGACA TCATCTATAA TTATCATGGG 
ATAGTTTCCT TGAAACTGGA GGATGACAGT TTCCCAACTC ACAAAAGGAA GGCCAAAGTA 
TCCATCATTA GTCAGCCACA AAAGACAATC AAAGTGGCAG AACTGCCTCA AGCAGATAAG 
GTGGAATCCA CAACTGACTC ACACTTCCCC AGACAGGACC AGTTGCCCTC ATTTCCAAAG 
AACTGCACTC TGGAATTAAA GGGACTCTTC CATTTTGAAG AAGGCATCCA GAAGCTGTAT 
CAGTGCAATG GGATCGCCTG GAAAGCCTGG AGTCCCCAAA CCAAGATACC TGGGCAACCT 
TGTAACTGTA TTCTCCAGGC AGCACATGCG GTGGCTCTGG GACATTGGTG GGAGAAAGTC 
CTTTTGGATA GGTTTGAACG ACCAAGTGCA TGCTGGCCAC TGGGAGTGGA TCGGTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FREM1 ( XM_017014325.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016869814.1 Homo sapiens FRAS1 related extracellular matrix 1 (FREM1), transcript variant X10, mRNA.

Price & Availability↑

CloneID	OHu98461
Clone ID Related Accession (Same CDS sequence)	XM_017014325.1 , XM_017014325.2
Accession Version	XM_017014325.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6300bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	FRAS1-related extracellular matrix protein 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017014325.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACTCTC TGAGTTGGGG GGCTGCGAAT GCCGTGCTGC TGCTGCTCCT CCTGGCCTGG 
GCCAGCCCCA CCTTCATCAG CATCAACCGC GGGGTGAGGG TGATGAAGGG CCACTCTGCC 
TTCCTGTCAG GAGATGACCT GAAGTTTGCC ATCCCTAAAG AGAAAGATGC CTGCAAAGTG 
GAAGTTGTGA TGAATGAGCC AATAACCCAG AGGGTTGGGA AACTCACTCC ACAGGTCTTT 
GACTGCCATT TCCTTCCCAA CGAAGTCAAG TATGTTCACA ATGGTTGTCC AATTCTTGAT 
GAAGACACAG TGAAGCTCAG ACTTTACAGA TTTACTGAAA GAGATACCTT CATAGAAACT 
TTTATCCTGT GGGTCTATCT CCTGGAACCA GACTGTAACA TCATCCATAT GAGTAACAAT 
GTGCTGGAGG TGCCTGAATT CAATGGCTTG TCCCAAGCGA TTGATAAAAA TCTGCTCAGA 
TTCGATTATG ATAGGATGGC TAGCCTGGAA TGTACCGTCA GCCTGGACAC TGCGAGAACT 
CGGCTGCCAG CCCATGGCCA GATGGTTCTC GGGGAGCCTC GACCAGAAGA ACCTCGTGGA 
GATCAGCCAC ACAGTTTTTT CCCAGAGTCC CGTATGATGT CTTTTAATGC CTTTCTGGAA 
CTGAGAGCAA AACTGAAGTG TCCAGGTGGG AGCTGTACCC CAGGATTAAA GAAAATAGGA 
AGCCTCAAAG TGAGCTGTGA GGAGTTCCTG CTGATGGGCC TTCGTTATCA GCATCTGGAT 
CCCCCTTCAC CCAACATTGA TTATATCTCC ATCCAACTGG ACCTCACAGA TACCAGGAGC 
AAAATTGTGT ACAAGTCAGA GAGTGCGTGG CTGCCTGTCT ATATCAGAGC TGGAATTCCG 
AATCAGATTC CAAAGGCTGC ATTCATGGCC GTGTTTATTC TGGAAGTGGA TCAGTTCATC 
CTGACCTCCT TGACTACATC AGTTCTGGAC TGTGAAGAAG ATGAGACCCC TAAACCCTTG 
CTGGTGTTCA ACATTACTAA AGCCCCGCTC CAGGGCTATG TGACTCACCT GTTGGATCAC 
ACCAGACCAA TCTCCTCATT CACCTGGAAA GATCTCAGTG ACATGCAGAT CGCCTATCAG 
CCACCAAACA GCAGCCATTC TGAGAGGAGA CATGATGAGG TAGAATTGGA GGTATACGAC 
TTCTTCTTTG AAAGGAGTGC ACCTATGACA GTCCACATCT CCATCAGAAC AGCAGATACA 
AATGCCCCCC GTGTATCCTG GAATACAGGT CTGAGTCTCC TTGAGGGGCA GTCTCGAGCC 
ATCACTTGGG AACAGTTTCA GGTTGTCGAC AATGACGACA TTGGTGCTGT CCGGCTAGTC 
ACCGTTGGTG GCCTGCAGCA TGGATGGCTG ACTTTAAGAG GGGGGAAAGG GTTTCTCTTC 
ACCGTGGCTG ACCTCCAGGC TGGAGTTGTT CGCTATCATC ATGATGACAG CGACTCCACC 
AAAGACTTCG TGGTCTTCCG GATATTTGAT GGCCATCACA GCATCCGTCA CAAATTCCCC 
ATCAACGTCT TGCCCAAAGA TGATAGTCCC CCGTTCCTCA TAACCAATGT TGTGATTGAA 
CTGGAGGAGG GGCAGACCAT CCTGATCCAG GGATCCATGC TGCGAGCTTC AGATGTGGAC 
GCCAGTGATG ACTACATCTT CTTCAATATC ACAAAGCCTC CACAGGCTGG GGAGATCATG 
AAGAAGCCAG GGCCAGGACT GATAGGCTAT CCTGTCCATG GCTTCCTTCA GAGGGATTTG 
TTTAATGGAA TCATTTATTA TCGTCATTTT GGTGGAGAAA TCTTTGAAGA TTCTTTTCAA 
TTTGTCCTGT GGGACAGCCA TGAACCTCCA AATCTTTCAG TGCCACAGGT GGCAACAATC 
CATATAACTC CAGTGGATGA CCAGCTTCCA AAAGAGGCTC CTGGAGTTTC TCGGCATTTG 
GTTGTCAAGG AAACTGAGGT GGCCTATATA ACTAAGAAAC AGCTACATTT TATAGATTCA 
GAATCATATG ACAGGGAGCT GGTCTACACA ATAACTACTC CTCCATTTTT CTCCTTCAGC 
CACAGACACT TGGATGCTGG GAAATTATTT ATGGTGGACA GCATACCAAA AGTAGTTAAG 
AATCCTACGG CCCTGGAGCT GAGGTCATTC ACTCAGCATG CTGTGAACTA TATGAAAGTG 
GCCTACATGC CCCCCATGCA AGACATTGGT CCCCATTGCA GAGATGTCCA GTTCACATTT 
TCTGTCAGTA ACCAACATGG CGGTACTTTG CATGGGATCT GCTTTAACAT TACAATCCTC 
CCAGTGGACA ATCAAGTTCC TGAGGCGTTC ACCAACCCTC TGAAAGTGAC TGAGGGAGGT 
CAAAGCATCA TCAGCACAGA GCACATTCTA ATTTCTGATG CAGATACCAA GCTGGACAAT 
ATTGACCTCT CCCTGCGGGA ATTGCCTCTG CACGGAAGGG TGGAGCTGAA TGGATTTCCT 
CTAAATTCAG GGGGCACATT TTCTTGGGGC GATCTCCATA CCTTAAAAGT TAGGTATCAA 
CATGATGGAA CTGAAGTTCT TCAGGATGAC CTACTCTTGG AGGTCACCGA TGGCACAAAT 
TCAGCAGAAT TTGTACTACA TGTTGAGGTA TTCCCTGTCA ACGATGAGCC ACCAGTCTTA 
AAGGCTGACC TCATGCCTGT CATGAATTGC TCAGAGGGAG GAGAGGTGGT CATCACCTCT 
GAATACATTT TTGCTACTGA TGTGGACAGC GATAACTTGA AGTTGATGTT TGTGATTGCT 
CGCGAACCTC AGCATGGGGT GGTGAGGAGA GCTGGAGTCA CAGTGGATCA GTTCTCTCAG 
AGAGATGTTA TCTCAGAGGC CGTGACATAC AAACACACAG GTGGCGAGAT TGGCCTGATG 
CCTTGTTTTG ACACCATTAC ATTGGTGGTT TCGGATGGAG AGGCTGGCCC TTTTGTGAAT 
GGCTGTTGCT ACAATGGACC CAATCCATCT GTTCCACTTC ATGCGTCCTT TCCAGTGTAT 
GATCTCAACA TCACGGTATA CCCAGTAGAC AACCAGCCAC CTTCCATTGC AATAGGTCCC 
GTGTTTGTTG TAGATGAGGG CTGCTCAACA GCCCTTACTG TTAACCATTT GTCCGCCACT 
GACCCTGACA CTGCAGCAGA TGACTTGGAA TTTGTTTTGG TTTCTCCTCC TCAGTTTGGC 
TACCTCGAAA ATATACTCCC TTCTGTGGGT TTTGAAAAAA GCAATATTGG CATAAGTATA 
GATTCATTTC AGTGGAAAGA CATGAACGCT TTTCACATTA ACTATGTGCA GTCCAGGCAT 
CTGAGGATAG AACCAACTGC CGACCAGTTC ACGGTGTACG TCACAGATGG GAAGCATCAC 
TCCTTGGAGA TACCATTTTC TATTATAATC AACCCCACAA ATGATGAAGC TCCTGACTTT 
GTAGTGCAGA ATATTACTGT GTGTGAGGGT CAGATGAAAG AGCTGGACTC TTCCATCATC 
AGCGCTGTGG ACCTGGACAT TCCCCAGGAT GCCCTGCTGT TCAGCATCAC TCAAAAGCCA 
CGCCATGGCC TCCTCATCGA TAGGGGGTTT AGCAAAGACT TCTCTGAGAA TAAGCAGCCA 
GCCAACCCTC ACCAGAAACA TGCACCTGTT CACAGCTTTT CCATGGAACT CCTCAAGACT 
GGAATGAGGT TGACGTACAT GCATGATGAC TCAGAGAGCC TTGCTGATGA TTTTACAATC 
CAATTGTCAG ATGGGAAACA TAAGATACTT AAAACCATTT CAGTAGAGGT CATCCCAGTT 
AATGATGAAA AACCAATGCT GAGCAAGAAG GCTGAAATTG CAATGAATAT GGGTGAAACT 
CGTATTATTT CCAGTGCTAT TCTTTCAGCC ATAGATGAAG ACTCACCCAG GGAGAAGATT 
TACTATGTAT TTGAAAGGCT TCCCCAAAAT GGGCAACTTC AGCTTAAGAT AGGGAGGGAC 
TGGGTTCCTC TCTCCCCTGG CATGAAATGC ACTCAGGAGG AAGTGGATCT GAACTTGCTG 
AGATACACAC ACACCGGGGC AATGGATTCC CAGAATCAAG ATAGCTTCAC CTTCTACCTT 
TGGGATGGCA ACAACAGGTC CCCTGCTCTT GACTGTCAAA TCACCATCAA GGACATGGAA 
AAAGGTGATA TTGTCATCCT TACAAAACCA CTCGTGGTGT CTAAAGGTGA TAGAGGTTTC 
TTAACAACCA CCACGCTCCT GGCTGTGGAC GGAACAGACA AGCCTGAGGA ACTGCTCTAT 
GTCATCACCT CCCCTCCGCG ATATGGCCAG ATCGAATATG TTCACTATCC TGGAGTTCCC 
ATTACAAACT TCAGCCAAAT GGATGTAGTG GGGCAGACAG TTTGCTATGT ACACAAGAGC 
AAGGTGACTG TCTCCAGTGA CAGATTCAGA TTCATCATCA GCAATGGACT GCGGACCGAG 
CACGGGGTGT TTGAGATCAC ACTGGAGACT GTGGACAGAG CCCTGCCTGT GGTAACCAGG 
AACAAGGGGT TGAGACTGGC CCAAGGGGCC GTGGGCCTGC TTTCCCCTGA CCTCCTTCAG 
CTGACCGACC CTGATACACC TGCGGAGAAC CTCACCTTCC TCTTGGTTCA GCTCCCCCAG 
CATGGCCAGC TCTACCTGTG GGGGACAGGG CTACTTCAAC ACAATTTCAC CCAGCAGGAT 
GTGGACAGCA AGAATGTGGC CTATCGGCAC TCAGGAGGGG ACTCCCAGAC TGACTGCTTT 
ACTTTCATGG CCACAGATGG GACAAACCAA GGCTTTATTG TGAATGGGAG AGTGTGGGAA 
GAACCTGTTT TATTCACCAT TCAGGTAGAC CAATTGGACA AAACAGCTCC TCGTATCACA 
CTCTTGCATT CCCCTTCTCA AGTGGGGCTC CTGAAAAATG GCTGCTACGG GATTTACATC 
ACTTCCCGCG TGTTGAAGGC ATCAGACCCT GACACTGAGG ACGATCAGAT CATCTTTAAA 
ATTCTACAAG GCCCAAAACA TGGACATCTG GAGAACACAA CAACAGGTGA ATTTATCCAT 
GAGAAATTTA GCCAAAAGGA CTTAAACAGT AAGACTATTC TTTACATCAT AAACCCATCT 
TTGGAAGTAA ATTCAGATAC CGTGGAATTT CAAATCATGG ACCCCACAGG GAACTCGGCC 
ACTCCTCAAA TTTTGGAACT GAAGTGGTCT CATATTGAAT GGTCACAGAC CGAATATGAA 
GTCTGTGAGA ATGTGGGTTT GTTGCCCTTG GAAATTATCA GAAGGGGATA TTCCATGGAC 
TCGGCCTTTG TGGGTATAAA GGTCAACCAA GTGTCAGCTG CAGTTGGAAA AGATTTCACC 
GTGATTCCAT CTAAACTGAT TCAGTTTGAC CCAGGAATGT CAACTAAGAT GTGGAATATA 
GCAATTACCT ATGACGGATT AGAGGAAGAT GATGAGGTCT TTGAAGTAAT TCTGAACTCC 
CCTGTGAATG CAGTTCTTGG CACAAAGACA AAAGCTGCAG TGAAAATTTT GGACTCAAAA 
GGAGGACAAT GCCATCCTTC ATATTCCTCC AACCAAAGCA AGCACAGCAC ATGGGAGAAG 
GGCATTTGGC ATCTGCTGCC CCCAGGGTCT TCCTCATCCA CCACTTCTGG TTCCTTTCAT 
CTGGAAAGAA GACCTCTTCC ATCTTCCATG CAGCTAGCAG TCATCAGGGG AGACACCCTG 
CGGGGCTTTG ATTCTACAGA TCTTTCTCAA AGGAAGCTTA GGACCCGTGG GAATGGCAAA 
ACAGTTCGTC CATCCTCTGT TTATAGAAAT GGAACAGACA TCATCTATAA TTATCATGGG 
ATAGTTTCCT TGAAACTGGA GGATGACAGT TTCCCAACTC ACAAAAGGAA GGCCAAAGTA 
TCCATCATTA GTCAGCCACA AAAGACAATC AAAGTGGCAG AACTGCCTCA AGCAGATAAG 
GTGGAATCCA CAACTGACTC ACACTTCCCC AGACAGGACC AGTTGCCCTC ATTTCCAAAG 
AACTGCACTC TGGAATTAAA GGGACTCTTC CATTTTGAAG AAGGCATCCA GAAGCTGTAT 
CAGTGCAATG GGATCGCCTG GAAAGCCTGG AGTCCCCAAA CCAAGATACC TGGGCAACCT 
TGTAACTGTA TTCTCCAGGC AGCACATGCG GTGGCTCTGG GACATTGGTG GGAGAAAGTC 
CTTTTGGATA GGTTTGAACG ACCAAGTGCA TGCTGGCCAC TGGGAGTGGA TCGGTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016869814.1
CDS	787..7086
Translation	MNSLSWGAANAVLLLLLLAWASPTFISINRGVRVMKGHSAFLSGDDLKFAIPKEKDACKVEVVMNEPITQRVGKLTPQVFDCHFLPNEVKYVHNGCPILDEDTVKLRLYRFTERDTFIETFILWVYLLEPDCNIIHMSNNVLEVPEFNGLSQAIDKNLLRFDYDRMASLECTVSLDTARTRLPAHGQMVLGEPRPEEPRGDQPHSFFPESRMMSFNAFLELRAKLKCPGGSCTPGLKKIGSLKVSCEEFLLMGLRYQHLDPPSPNIDYISIQLDLTDTRSKIVYKSESAWLPVYIRAGIPNQIPKAAFMAVFILEVDQFILTSLTTSVLDCEEDETPKPLLVFNITKAPLQGYVTHLLDHTRPISSFTWKDLSDMQIAYQPPNSSHSERRHDEVELEVYDFFFERSAPMTVHISIRTADTNAPRVSWNTGLSLLEGQSRAITWEQFQVVDNDDIGAVRLVTVGGLQHGWLTLRGGKGFLFTVADLQAGVVRYHHDDSDSTKDFVVFRIFDGHHSIRHKFPINVLPKDDSPPFLITNVVIELEEGQTILIQGSMLRASDVDASDDYIFFNITKPPQAGEIMKKPGPGLIGYPVHGFLQRDLFNGIIYYRHFGGEIFEDSFQFVLWDSHEPPNLSVPQVATIHITPVDDQLPKEAPGVSRHLVVKETEVAYITKKQLHFIDSESYDRELVYTITTPPFFSFSHRHLDAGKLFMVDSIPKVVKNPTALELRSFTQHAVNYMKVAYMPPMQDIGPHCRDVQFTFSVSNQHGGTLHGICFNITILPVDNQVPEAFTNPLKVTEGGQSIISTEHILISDADTKLDNIDLSLRELPLHGRVELNGFPLNSGGTFSWGDLHTLKVRYQHDGTEVLQDDLLLEVTDGTNSAEFVLHVEVFPVNDEPPVLKADLMPVMNCSEGGEVVITSEYIFATDVDSDNLKLMFVIAREPQHGVVRRAGVTVDQFSQRDVISEAVTYKHTGGEIGLMPCFDTITLVVSDGEAGPFVNGCCYNGPNPSVPLHASFPVYDLNITVYPVDNQPPSIAIGPVFVVDEGCSTALTVNHLSATDPDTAADDLEFVLVSPPQFGYLENILPSVGFEKSNIGISIDSFQWKDMNAFHINYVQSRHLRIEPTADQFTVYVTDGKHHSLEIPFSIIINPTNDEAPDFVVQNITVCEGQMKELDSSIISAVDLDIPQDALLFSITQKPRHGLLIDRGFSKDFSENKQPANPHQKHAPVHSFSMELLKTGMRLTYMHDDSESLADDFTIQLSDGKHKILKTISVEVIPVNDEKPMLSKKAEIAMNMGETRIISSAILSAIDEDSPREKIYYVFERLPQNGQLQLKIGRDWVPLSPGMKCTQEEVDLNLLRYTHTGAMDSQNQDSFTFYLWDGNNRSPALDCQITIKDMEKGDIVILTKPLVVSKGDRGFLTTTTLLAVDGTDKPEELLYVITSPPRYGQIEYVHYPGVPITNFSQMDVVGQTVCYVHKSKVTVSSDRFRFIISNGLRTEHGVFEITLETVDRALPVVTRNKGLRLAQGAVGLLSPDLLQLTDPDTPAENLTFLLVQLPQHGQLYLWGTGLLQHNFTQQDVDSKNVAYRHSGGDSQTDCFTFMATDGTNQGFIVNGRVWEEPVLFTIQVDQLDKTAPRITLLHSPSQVGLLKNGCYGIYITSRVLKASDPDTEDDQIIFKILQGPKHGHLENTTTGEFIHEKFSQKDLNSKTILYIINPSLEVNSDTVEFQIMDPTGNSATPQILELKWSHIEWSQTEYEVCENVGLLPLEIIRRGYSMDSAFVGIKVNQVSAAVGKDFTVIPSKLIQFDPGMSTKMWNIAITYDGLEEDDEVFEVILNSPVNAVLGTKTKAAVKILDSKGGQCHPSYSSNQSKHSTWEKGIWHLLPPGSSSSTTSGSFHLERRPLPSSMQLAVIRGDTLRGFDSTDLSQRKLRTRGNGKTVRPSSVYRNGTDIIYNYHGIVSLKLEDDSFPTHKRKAKVSIISQPQKTIKVAELPQADKVESTTDSHFPRQDQLPSFPKNCTLELKGLFHFEEGIQKLYQCNGIAWKAWSPQTKIPGQPCNCILQAAHAVALGHWWEKVLLDRFERPSACWPLGVDRW

Target ORF information:

RefSeq Version	XM_017014325.2
Organism	Homo sapiens(human)
Definition	Homo sapiens FRAS1 related extracellular matrix 1 (FREM1), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017014325.2

ORF Insert Sequence:

ATGAACTCTC TGAGTTGGGG GGCTGCGAAT GCCGTGCTGC TGCTGCTCCT CCTGGCCTGG 
GCCAGCCCCA CCTTCATCAG CATCAACCGC GGGGTGAGGG TGATGAAGGG CCACTCTGCC 
TTCCTGTCAG GAGATGACCT GAAGTTTGCC ATCCCTAAAG AGAAAGATGC CTGCAAAGTG 
GAAGTTGTGA TGAATGAGCC AATAACCCAG AGGGTTGGGA AACTCACTCC ACAGGTCTTT 
GACTGCCATT TCCTTCCCAA CGAAGTCAAG TATGTTCACA ATGGTTGTCC AATTCTTGAT 
GAAGACACAG TGAAGCTCAG ACTTTACAGA TTTACTGAAA GAGATACCTT CATAGAAACT 
TTTATCCTGT GGGTCTATCT CCTGGAACCA GACTGTAACA TCATCCATAT GAGTAACAAT 
GTGCTGGAGG TGCCTGAATT CAATGGCTTG TCCCAAGCGA TTGATAAAAA TCTGCTCAGA 
TTCGATTATG ATAGGATGGC TAGCCTGGAA TGTACCGTCA GCCTGGACAC TGCGAGAACT 
CGGCTGCCAG CCCATGGCCA GATGGTTCTC GGGGAGCCTC GACCAGAAGA ACCTCGTGGA 
GATCAGCCAC ACAGTTTTTT CCCAGAGTCC CGTATGATGT CTTTTAATGC CTTTCTGGAA 
CTGAGAGCAA AACTGAAGTG TCCAGGTGGG AGCTGTACCC CAGGATTAAA GAAAATAGGA 
AGCCTCAAAG TGAGCTGTGA GGAGTTCCTG CTGATGGGCC TTCGTTATCA GCATCTGGAT 
CCCCCTTCAC CCAACATTGA TTATATCTCC ATCCAACTGG ACCTCACAGA TACCAGGAGC 
AAAATTGTGT ACAAGTCAGA GAGTGCGTGG CTGCCTGTCT ATATCAGAGC TGGAATTCCG 
AATCAGATTC CAAAGGCTGC ATTCATGGCC GTGTTTATTC TGGAAGTGGA TCAGTTCATC 
CTGACCTCCT TGACTACATC AGTTCTGGAC TGTGAAGAAG ATGAGACCCC TAAACCCTTG 
CTGGTGTTCA ACATTACTAA AGCCCCGCTC CAGGGCTATG TGACTCACCT GTTGGATCAC 
ACCAGACCAA TCTCCTCATT CACCTGGAAA GATCTCAGTG ACATGCAGAT CGCCTATCAG 
CCACCAAACA GCAGCCATTC TGAGAGGAGA CATGATGAGG TAGAATTGGA GGTATACGAC 
TTCTTCTTTG AAAGGAGTGC ACCTATGACA GTCCACATCT CCATCAGAAC AGCAGATACA 
AATGCCCCCC GTGTATCCTG GAATACAGGT CTGAGTCTCC TTGAGGGGCA GTCTCGAGCC 
ATCACTTGGG AACAGTTTCA GGTTGTCGAC AATGACGACA TTGGTGCTGT CCGGCTAGTC 
ACCGTTGGTG GCCTGCAGCA TGGATGGCTG ACTTTAAGAG GGGGGAAAGG GTTTCTCTTC 
ACCGTGGCTG ACCTCCAGGC TGGAGTTGTT CGCTATCATC ATGATGACAG CGACTCCACC 
AAAGACTTCG TGGTCTTCCG GATATTTGAT GGCCATCACA GCATCCGTCA CAAATTCCCC 
ATCAACGTCT TGCCCAAAGA TGATAGTCCC CCGTTCCTCA TAACCAATGT TGTGATTGAA 
CTGGAGGAGG GGCAGACCAT CCTGATCCAG GGATCCATGC TGCGAGCTTC AGATGTGGAC 
GCCAGTGATG ACTACATCTT CTTCAATATC ACAAAGCCTC CACAGGCTGG GGAGATCATG 
AAGAAGCCAG GGCCAGGACT GATAGGCTAT CCTGTCCATG GCTTCCTTCA GAGGGATTTG 
TTTAATGGAA TCATTTATTA TCGTCATTTT GGTGGAGAAA TCTTTGAAGA TTCTTTTCAA 
TTTGTCCTGT GGGACAGCCA TGAACCTCCA AATCTTTCAG TGCCACAGGT GGCAACAATC 
CATATAACTC CAGTGGATGA CCAGCTTCCA AAAGAGGCTC CTGGAGTTTC TCGGCATTTG 
GTTGTCAAGG AAACTGAGGT GGCCTATATA ACTAAGAAAC AGCTACATTT TATAGATTCA 
GAATCATATG ACAGGGAGCT GGTCTACACA ATAACTACTC CTCCATTTTT CTCCTTCAGC 
CACAGACACT TGGATGCTGG GAAATTATTT ATGGTGGACA GCATACCAAA AGTAGTTAAG 
AATCCTACGG CCCTGGAGCT GAGGTCATTC ACTCAGCATG CTGTGAACTA TATGAAAGTG 
GCCTACATGC CCCCCATGCA AGACATTGGT CCCCATTGCA GAGATGTCCA GTTCACATTT 
TCTGTCAGTA ACCAACATGG CGGTACTTTG CATGGGATCT GCTTTAACAT TACAATCCTC 
CCAGTGGACA ATCAAGTTCC TGAGGCGTTC ACCAACCCTC TGAAAGTGAC TGAGGGAGGT 
CAAAGCATCA TCAGCACAGA GCACATTCTA ATTTCTGATG CAGATACCAA GCTGGACAAT 
ATTGACCTCT CCCTGCGGGA ATTGCCTCTG CACGGAAGGG TGGAGCTGAA TGGATTTCCT 
CTAAATTCAG GGGGCACATT TTCTTGGGGC GATCTCCATA CCTTAAAAGT TAGGTATCAA 
CATGATGGAA CTGAAGTTCT TCAGGATGAC CTACTCTTGG AGGTCACCGA TGGCACAAAT 
TCAGCAGAAT TTGTACTACA TGTTGAGGTA TTCCCTGTCA ACGATGAGCC ACCAGTCTTA 
AAGGCTGACC TCATGCCTGT CATGAATTGC TCAGAGGGAG GAGAGGTGGT CATCACCTCT 
GAATACATTT TTGCTACTGA TGTGGACAGC GATAACTTGA AGTTGATGTT TGTGATTGCT 
CGCGAACCTC AGCATGGGGT GGTGAGGAGA GCTGGAGTCA CAGTGGATCA GTTCTCTCAG 
AGAGATGTTA TCTCAGAGGC CGTGACATAC AAACACACAG GTGGCGAGAT TGGCCTGATG 
CCTTGTTTTG ACACCATTAC ATTGGTGGTT TCGGATGGAG AGGCTGGCCC TTTTGTGAAT 
GGCTGTTGCT ACAATGGACC CAATCCATCT GTTCCACTTC ATGCGTCCTT TCCAGTGTAT 
GATCTCAACA TCACGGTATA CCCAGTAGAC AACCAGCCAC CTTCCATTGC AATAGGTCCC 
GTGTTTGTTG TAGATGAGGG CTGCTCAACA GCCCTTACTG TTAACCATTT GTCCGCCACT 
GACCCTGACA CTGCAGCAGA TGACTTGGAA TTTGTTTTGG TTTCTCCTCC TCAGTTTGGC 
TACCTCGAAA ATATACTCCC TTCTGTGGGT TTTGAAAAAA GCAATATTGG CATAAGTATA 
GATTCATTTC AGTGGAAAGA CATGAACGCT TTTCACATTA ACTATGTGCA GTCCAGGCAT 
CTGAGGATAG AACCAACTGC CGACCAGTTC ACGGTGTACG TCACAGATGG GAAGCATCAC 
TCCTTGGAGA TACCATTTTC TATTATAATC AACCCCACAA ATGATGAAGC TCCTGACTTT 
GTAGTGCAGA ATATTACTGT GTGTGAGGGT CAGATGAAAG AGCTGGACTC TTCCATCATC 
AGCGCTGTGG ACCTGGACAT TCCCCAGGAT GCCCTGCTGT TCAGCATCAC TCAAAAGCCA 
CGCCATGGCC TCCTCATCGA TAGGGGGTTT AGCAAAGACT TCTCTGAGAA TAAGCAGCCA 
GCCAACCCTC ACCAGAAACA TGCACCTGTT CACAGCTTTT CCATGGAACT CCTCAAGACT 
GGAATGAGGT TGACGTACAT GCATGATGAC TCAGAGAGCC TTGCTGATGA TTTTACAATC 
CAATTGTCAG ATGGGAAACA TAAGATACTT AAAACCATTT CAGTAGAGGT CATCCCAGTT 
AATGATGAAA AACCAATGCT GAGCAAGAAG GCTGAAATTG CAATGAATAT GGGTGAAACT 
CGTATTATTT CCAGTGCTAT TCTTTCAGCC ATAGATGAAG ACTCACCCAG GGAGAAGATT 
TACTATGTAT TTGAAAGGCT TCCCCAAAAT GGGCAACTTC AGCTTAAGAT AGGGAGGGAC 
TGGGTTCCTC TCTCCCCTGG CATGAAATGC ACTCAGGAGG AAGTGGATCT GAACTTGCTG 
AGATACACAC ACACCGGGGC AATGGATTCC CAGAATCAAG ATAGCTTCAC CTTCTACCTT 
TGGGATGGCA ACAACAGGTC CCCTGCTCTT GACTGTCAAA TCACCATCAA GGACATGGAA 
AAAGGTGATA TTGTCATCCT TACAAAACCA CTCGTGGTGT CTAAAGGTGA TAGAGGTTTC 
TTAACAACCA CCACGCTCCT GGCTGTGGAC GGAACAGACA AGCCTGAGGA ACTGCTCTAT 
GTCATCACCT CCCCTCCGCG ATATGGCCAG ATCGAATATG TTCACTATCC TGGAGTTCCC 
ATTACAAACT TCAGCCAAAT GGATGTAGTG GGGCAGACAG TTTGCTATGT ACACAAGAGC 
AAGGTGACTG TCTCCAGTGA CAGATTCAGA TTCATCATCA GCAATGGACT GCGGACCGAG 
CACGGGGTGT TTGAGATCAC ACTGGAGACT GTGGACAGAG CCCTGCCTGT GGTAACCAGG 
AACAAGGGGT TGAGACTGGC CCAAGGGGCC GTGGGCCTGC TTTCCCCTGA CCTCCTTCAG 
CTGACCGACC CTGATACACC TGCGGAGAAC CTCACCTTCC TCTTGGTTCA GCTCCCCCAG 
CATGGCCAGC TCTACCTGTG GGGGACAGGG CTACTTCAAC ACAATTTCAC CCAGCAGGAT 
GTGGACAGCA AGAATGTGGC CTATCGGCAC TCAGGAGGGG ACTCCCAGAC TGACTGCTTT 
ACTTTCATGG CCACAGATGG GACAAACCAA GGCTTTATTG TGAATGGGAG AGTGTGGGAA 
GAACCTGTTT TATTCACCAT TCAGGTAGAC CAATTGGACA AAACAGCTCC TCGTATCACA 
CTCTTGCATT CCCCTTCTCA AGTGGGGCTC CTGAAAAATG GCTGCTACGG GATTTACATC 
ACTTCCCGCG TGTTGAAGGC ATCAGACCCT GACACTGAGG ACGATCAGAT CATCTTTAAA 
ATTCTACAAG GCCCAAAACA TGGACATCTG GAGAACACAA CAACAGGTGA ATTTATCCAT 
GAGAAATTTA GCCAAAAGGA CTTAAACAGT AAGACTATTC TTTACATCAT AAACCCATCT 
TTGGAAGTAA ATTCAGATAC CGTGGAATTT CAAATCATGG ACCCCACAGG GAACTCGGCC 
ACTCCTCAAA TTTTGGAACT GAAGTGGTCT CATATTGAAT GGTCACAGAC CGAATATGAA 
GTCTGTGAGA ATGTGGGTTT GTTGCCCTTG GAAATTATCA GAAGGGGATA TTCCATGGAC 
TCGGCCTTTG TGGGTATAAA GGTCAACCAA GTGTCAGCTG CAGTTGGAAA AGATTTCACC 
GTGATTCCAT CTAAACTGAT TCAGTTTGAC CCAGGAATGT CAACTAAGAT GTGGAATATA 
GCAATTACCT ATGACGGATT AGAGGAAGAT GATGAGGTCT TTGAAGTAAT TCTGAACTCC 
CCTGTGAATG CAGTTCTTGG CACAAAGACA AAAGCTGCAG TGAAAATTTT GGACTCAAAA 
GGAGGACAAT GCCATCCTTC ATATTCCTCC AACCAAAGCA AGCACAGCAC ATGGGAGAAG 
GGCATTTGGC ATCTGCTGCC CCCAGGGTCT TCCTCATCCA CCACTTCTGG TTCCTTTCAT 
CTGGAAAGAA GACCTCTTCC ATCTTCCATG CAGCTAGCAG TCATCAGGGG AGACACCCTG 
CGGGGCTTTG ATTCTACAGA TCTTTCTCAA AGGAAGCTTA GGACCCGTGG GAATGGCAAA 
ACAGTTCGTC CATCCTCTGT TTATAGAAAT GGAACAGACA TCATCTATAA TTATCATGGG 
ATAGTTTCCT TGAAACTGGA GGATGACAGT TTCCCAACTC ACAAAAGGAA GGCCAAAGTA 
TCCATCATTA GTCAGCCACA AAAGACAATC AAAGTGGCAG AACTGCCTCA AGCAGATAAG 
GTGGAATCCA CAACTGACTC ACACTTCCCC AGACAGGACC AGTTGCCCTC ATTTCCAAAG 
AACTGCACTC TGGAATTAAA GGGACTCTTC CATTTTGAAG AAGGCATCCA GAAGCTGTAT 
CAGTGCAATG GGATCGCCTG GAAAGCCTGG AGTCCCCAAA CCAAGATACC TGGGCAACCT 
TGTAACTGTA TTCTCCAGGC AGCACATGCG GTGGCTCTGG GACATTGGTG GGAGAAAGTC 
CTTTTGGATA GGTTTGAACG ACCAAGTGCA TGCTGGCCAC TGGGAGTGGA TCGGTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FREM1 ( XM_017014326.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016869815.1 Homo sapiens FRAS1 related extracellular matrix 1 (FREM1), transcript variant X11, mRNA.

Price & Availability↑

CloneID	OHu98462
Clone ID Related Accession (Same CDS sequence)	XM_017014326.1
Accession Version	XM_017014326.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6159bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	FRAS1-related extracellular matrix protein 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTAACA ATGTGCTGGA GGTGCCTGAA TTCAATGGCT TGTCCCAAGC GATTGATAAA 
AATCTGCTCA GATTCGATTA TGATAGGATG GCTAGCCTGG AATGTACCGT CAGCCTGGAC 
ACTGCGAGAA CTCGGCTGCC AGCCCATGGC CAGATGGTTC TCGGGGAGCC TCGACCAGAA 
GAACCTCGTG GAGATCAGCC ACACAGTTTT TTCCCAGAGT CCCGTATGAT GTCTTTTAAT 
GCCTTTCTGG AACTGAGAGC AAAACTGAAG TGTCCAGGTG GGAGCTGTAC CCCAGGATTA 
AAGAAAATAG GAAGCCTCAA AGTGAGCTGT GAGGAGTTCC TGCTGATGGG CCTTCGTTAT 
CAGCATCTGG ATCCCCCTTC ACCCAACATT GATTATATCT CCATCCAACT GGACCTCACA 
GATACCAGGA GCAAAATTGT GTACAAGTCA GAGAGTGCGT GGCTGCCTGT CTATATCAGA 
GCTGGAATTC CGAATCAGAT TCCAAAGGCT GCATTCATGG CCGTGTTTAT TCTGGAAGTG 
GATCAGTTCA TCCTGACCTC CTTGACTACA TCAGTTCTGG ACTGTGAAGA AGATGAGACC 
CCTAAACCCT TGCTGGTGTT CAACATTACT AAAGCCCCGC TCCAGGGCTA TGTGACTCAC 
CTGTTGGATC ACACCAGACC AATCTCCTCA TTCACCTGGA AAGATCTCAG TGACATGCAG 
ATCGCCTATC AGCCACCAAA CAGCAGCCAT TCTGAGAGGA GACATGATGA GGTAGAATTG 
GAGGTATACG ACTTCTTCTT TGAAAGGAGT GCACCTATGA CAGTCCACAT CTCCATCAGA 
ACAGCAGATA CAAATGCCCC CCGTGTATCC TGGAATACAG GTCTGAGTCT CCTTGAGGGG 
CAGTCTCGAG CCATCACTTG GGAACAGTTT CAGGTTGTCG ACAATGACGA CATTGGTGCT 
GTCCGGCTAG TCACCGTTGG TGGCCTGCAG CATGGATGGC TGACTTTAAG AGGGGGGAAA 
GGGTTTCTCT TCACCGTGGC TGACCTCCAG GCTGGAGTTG TTCGCTATCA TCATGATGAC 
AGCGACTCCA CCAAAGACTT CGTGGTCTTC CGGATATTTG ATGGCCATCA CAGCATCCGT 
CACAAATTCC CCATCAACGT CTTGCCCAAA GATGATAGTC CCCCGTTCCT CATAACCAAT 
GTTGTGATTG AACTGGAGGA GGGGCAGACC ATCCTGATCC AGGGATCCAT GCTGCGAGCT 
TCAGATGTGG ACGCCAGTGA TGACTACATC TTCTTCAATA TCACAAAGCC TCCACAGGCT 
GGGGAGATCA TGAAGAAGCC AGGGCCAGGA CTGATAGGCT ATCCTGTCCA TGGCTTCCTT 
CAGAGGGATT TGTTTAATGG AATCATTTAT TATCGTCATT TTGGTGGAGA AATCTTTGAA 
GATTCTTTTC AATTTGTCCT GTGGGACAGC CATGAACCTC CAAATCTTTC AGTGCCACAG 
GTGGCAACAA TCCATATAAC TCCAGTGGAT GACCAGCTTC CAAAAGAGGC TCCTGGAGTT 
TCTCGGCATT TGGTTGTCAA GGAAACTGAG GTGGCCTATA TAACTAAGAA ACAGCTACAT 
TTTATAGATT CAGAATCATA TGACAGGGAG CTGGTCTACA CAATAACTAC TCCTCCATTT 
TTCTCCTTCA GCCACAGACA CTTGGATGCT GGGAAATTAT TTATGGTGGA CAGCATACCA 
AAAGTAGTTA AGAATCCTAC GGCCCTGGAG CTGAGGTCAT TCACTCAGCA TGCTGTGAAC 
TATATGAAAG TGGCCTACAT GCCCCCCATG CAAGACATTG GTCCCCATTG CAGAGATGTC 
CAGTTCACAT TTTCTGTCAG TAACCAACAT GGCGGTACTT TGCATGGGAT CTGCTTTAAC 
ATTACAATCC TCCCAGTGGA CAATCAAGTT CCTGAGGCGT TCACCAACCC TCTGAAAGTG 
ACTGAGGGAG GTCAAAGCAT CATCAGCACA GAGCACATTC TAATTTCTGA TGCAGATACC 
AAGCTGGACA ATATTGACCT CTCCCTGCGG GAATTGCCTC TGCACGGAAG GGTGGAGCTG 
AATGGATTTC CTCTAAATTC AGGGGGCACA TTTTCTTGGG GCGATCTCCA TACCTTAAAA 
GTTAGGTATC AACATGATGG AACTGAAGTT CTTCAGGATG ACCTACTCTT GGAGGTCACC 
GATGGCACAA ATTCAGCAGA ATTTGTACTA CATGTTGAGG TATTCCCTGT CAACGATGAG 
CCACCAGTCT TAAAGGCTGA CCTCATGCCT GTCATGAATT GCTCAGAGGG AGGAGAGGTG 
GTCATCACCT CTGAATACAT TTTTGCTACT GATGTGGACA GCGATAACTT GAAGTTGATG 
TTTGTGATTG CTCGCGAACC TCAGCATGGG GTGGTGAGGA GAGCTGGAGT CACAGTGGAT 
CAGTTCTCTC AGAGAGATGT TATCTCAGAG GCCGTGACAT ACAAACACAC AGGTGGCGAG 
ATTGGCCTGA TGCCTTGTTT TGACACCATT ACATTGGTGG TTTCGGATGG AGAGGCTGGC 
CCTTTTGTGA ATGGCTGTTG CTACAATGGA CCCAATCCAT CTGTTCCACT TCATGCGTCC 
TTTCCAGTGT ATGATCTCAA CATCACGGTA TACCCAGTAG ACAACCAGCC ACCTTCCATT 
GCAATAGGTC CCGTGTTTGT TGTAGATGAG GGCTGCTCAA CAGCCCTTAC TGTTAACCAT 
TTGTCCGCCA CTGACCCTGA CACTGCAGCA GATGACTTGG AATTTGTTTT GGTTTCTCCT 
CCTCAGTTTG GCTACCTCGA AAATATACTC CCTTCTGTGG GTTTTGAAAA AAGCAATATT 
GGCATAAGTA TAGATTCATT TCAGTGGAAA GACATGAACG CTTTTCACAT TAACTATGTG 
CAGTCCAGGC ATCTGAGGAT AGAACCAACT GCCGACCAGT TCACGGTGTA CGTCACAGAT 
GGGAAGCATC ACTCCTTGGA GATACCATTT TCTATTATAA TCAACCCCAC AAATGATGAA 
GCTCCTGACT TTGTAGTGCA GAATATTACT GTGTGTGAGG GTCAGATGAA AGAGCTGGAC 
TCTTCCATCA TCAGCGCTGT GGACCTGGAC ATTCCCCAGG ATGCCCTGCT GTTCAGCATC 
ACTCAAAAGC CACGCCATGG CCTCCTCATC GATAGGGGGT TTAGCAAAGA CTTCTCTGAG 
AATAAGCAGC CAGCCAACCC TCACCAGAAA CATGCACCTG TTCACAGCTT TTCCATGGAA 
CTCCTCAAGA CTGGAATGAG GTTGACGTAC ATGCATGATG ACTCAGAGAG CCTTGCTGAT 
GATTTTACAA TCCAATTGTC AGATGGGAAA CATAAGATAC TTAAAACCAT TTCAGTAGAG 
GTCATCCCAG TTAATGATGA AAAACCAATG CTGAGCAAGA AGGCTGAAAT TGCAATGAAT 
ATGGGTGAAA CTCGTATTAT TTCCAGTGCT ATTCTTTCAG CCATAGATGA AGACTCACCC 
AGGGAGAAGA TTTACTATGT ATTTGAAAGG CTTCCCCAAA ATGGGCAACT TCAGCTTAAG 
ATAGGGAGGG ACTGGGTTCC TCTCTCCCCT GGCATGAAAT GCACTCAGGA GGAAGTGGAT 
CTGAACTTGC TGAGATACAC ACACACCGGG GCAATGGATT CCCAGAATCA AGATAGCTTC 
ACCTTCTACC TTTGGGATGG CAACAACAGG TCCCCTGCTC TTGACTGTCA AATCACCATC 
AAGGACATGG AAAAAGGTGA TATTGTCATC CTTACAAAAC CACTCGTGGT GTCTAAAGGT 
GATAGAGGTT TCTTAACAAC CACCACGCTC CTGGCTGTGG ACGGAACAGA CAAGCCTGAG 
GAACTGCTCT ATGTCATCAC CTCCCCTCCG CGATATGGCC AGATCGAATA TGTTCACTAT 
CCTGGAGTTC CCATTACAAA CTTCAGCCAA ATGGATGTAG TGGGGCAGAC AGTTTGCTAT 
GTACACAAGA GCAAGGTGAC TGTCTCCAGT GACAGATTCA GATTCATCAT CAGCAATGGA 
CTGCGGACCG AGCACGGGGT GTTTGAGATC ACACTGGAGA CTGTGGACAG AGCCCTGCCT 
GTGGTAACCA GGAACAAGGG GTTGAGACTG GCCCAAGGGG CCGTGGGCCT GCTTTCCCCT 
GACCTCCTTC AGCTGACCGA CCCTGATACA CCTGCGGAGA ACCTCACCTT CCTCTTGGTT 
CAGCTCCCCC AGCATGGCCA GCTCTACCTG TGGGGGACAG GGCTACTTCA ACACAATTTC 
ACCCAGCAGG ATGTGGACAG CAAGAATGTG GCCTATCGGC ACTCAGGAGG GGACTCCCAG 
ACTGACTGCT TTACTTTCAT GGCCACAGAT GGGACAAACC AAGGCTTTAT TGTGAATGGG 
AGAGTGTGGG AAGAACCTGT TTTATTCACC ATTCAGGTAG ACCAATTGGA CAAAACAGCT 
CCTCGTATCA CACTCTTGCA TTCCCCTTCT CAAGTGGGGC TCCTGAAAAA TGGCTGCTAC 
GGGATTTACA TCACTTCCCG CGTGTTGAAG GCATCAGACC CTGACACTGA GGACGATCAG 
ATCATCTTTA AAATTCTACA AGGCCCAAAA CATGGACATC TGGAGAACAC AACAACAGGT 
GAATTTATCC ATGAGAAATT TAGCCAAAAG GACTTAAACA GTAAGACTAT TCTTTACATC 
ATAAACCCAT CTTTGGAAGT AAATTCAGAT ACCGTGGAAT TTCAAATCAT GGACCCCACA 
GGGAACTCGG CCACTCCTCA AATTTTGGAA CTGAAGTGGT CTCATATTGA ATGGTCACAG 
ACCGAATATG AAGTCTGTGA GAATGTGGGT TTGTTGCCCT TGGAAATTAT CAGAAGGGGA 
TATTCCATGG ACTCGGCCTT TGTGGGTATA AAGGTCAACC AAGTGTCAGC TGCAGTTGGA 
AAAGATTTCA CCGTGATTCC ATCTAAACTG ATTCAGTTTG ACCCAGGAAT GTCAACTAAG 
ATGTGGAATA TAGCAATTAC CTATGACGGA TTAGAGGAAG ATGATGAGGT CTTTGAAGTA 
ATTCTGAACT CCCCTGTGAA TGCAGTTCTT GGCACAAAGA CAAAAGCTGC AGTGAAAATT 
TTGGACTCAA AAGGAGGACA ATGCCATCCT TCATATTCCT CCAACCAAAG CAAGCACAGC 
ACATGGGAGA AGGGCATTTG GCATCTGCTG CCCCCAGGGT CTTCCTCATC CACCACTTCT 
GGTTCCTTTC ATCTGGAAAG AAGACCTCTT CCATCTTCCA TGCAGCTAGC AGTCATCAGG 
GGAGACACCC TGCGGGGCTT TGATTCTACA GATCTTTCTC AAAGGAAGCT TAGGACCCGT 
GGGAATGGCA AAACAGTTCG TCCATCCTCT GTTTATAGAA ATGGAACAGA CATCATCTAT 
AATTATCATG GGATAGTTTC CTTGAAACTG GAGGATGACA GTTTCCCAAC TCACAAAAGG 
AAGGCCAAAG TATCCATCAT TAGTCAGCCA CAAAAGACAA TCAAAGTGGC AGAACTGCCT 
CAAGCAGATA AGGTGGAATC CACAACTGAC TCACACTTCC CCAGACAGGA CCAGTTGCCC 
TCATTTCCAA AGAACTGCAC TCTGGAATTA AAGGGACTCT TCCATTTTGA AGAAGGCATC 
CAGAAGCTGT ATCAGTGCAA TGGGATCGCC TGGAAAGCCT GGAGTCCCCA AACCAAGGAT 
GTGGAAGACA AATCCTGTCC AGCCGGGTGG CACCAGCACT CAGGCTACTG TCACATCTTG 
ATCACAGAGC AGAAAGGCAC CTGGAATGCG GCTGCCCAAG CTTGCAGGGA ACAATACCTG 
GGCAACCTTG TAACTGTATT CTCCAGGCAG CACATGCGGT GGCTCTGGGA CATTGGTGGG 
AGAAAGTCCT TTTGGATAGG TTTGAACGAC CAAGTGCATG CTGGCCACTG GGAGTGGATC 
GGTGGTGAAC CTGTTGCCTT CACCAATGGG AGAAGAGGGC CCTCTCAACG CTCCAAGCTT 
GGAAAGAGCT GTGTTTTGGT TCAAAGACAA GGGAAATGGC AAACAAAAGA CTGTAGGAGA 
GCCAAACCTC ATAATTATGT GTGTTCCAGA AAACTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016869815.1
CDS	289..6447
Translation	MSNNVLEVPEFNGLSQAIDKNLLRFDYDRMASLECTVSLDTARTRLPAHGQMVLGEPRPEEPRGDQPHSFFPESRMMSFNAFLELRAKLKCPGGSCTPGLKKIGSLKVSCEEFLLMGLRYQHLDPPSPNIDYISIQLDLTDTRSKIVYKSESAWLPVYIRAGIPNQIPKAAFMAVFILEVDQFILTSLTTSVLDCEEDETPKPLLVFNITKAPLQGYVTHLLDHTRPISSFTWKDLSDMQIAYQPPNSSHSERRHDEVELEVYDFFFERSAPMTVHISIRTADTNAPRVSWNTGLSLLEGQSRAITWEQFQVVDNDDIGAVRLVTVGGLQHGWLTLRGGKGFLFTVADLQAGVVRYHHDDSDSTKDFVVFRIFDGHHSIRHKFPINVLPKDDSPPFLITNVVIELEEGQTILIQGSMLRASDVDASDDYIFFNITKPPQAGEIMKKPGPGLIGYPVHGFLQRDLFNGIIYYRHFGGEIFEDSFQFVLWDSHEPPNLSVPQVATIHITPVDDQLPKEAPGVSRHLVVKETEVAYITKKQLHFIDSESYDRELVYTITTPPFFSFSHRHLDAGKLFMVDSIPKVVKNPTALELRSFTQHAVNYMKVAYMPPMQDIGPHCRDVQFTFSVSNQHGGTLHGICFNITILPVDNQVPEAFTNPLKVTEGGQSIISTEHILISDADTKLDNIDLSLRELPLHGRVELNGFPLNSGGTFSWGDLHTLKVRYQHDGTEVLQDDLLLEVTDGTNSAEFVLHVEVFPVNDEPPVLKADLMPVMNCSEGGEVVITSEYIFATDVDSDNLKLMFVIAREPQHGVVRRAGVTVDQFSQRDVISEAVTYKHTGGEIGLMPCFDTITLVVSDGEAGPFVNGCCYNGPNPSVPLHASFPVYDLNITVYPVDNQPPSIAIGPVFVVDEGCSTALTVNHLSATDPDTAADDLEFVLVSPPQFGYLENILPSVGFEKSNIGISIDSFQWKDMNAFHINYVQSRHLRIEPTADQFTVYVTDGKHHSLEIPFSIIINPTNDEAPDFVVQNITVCEGQMKELDSSIISAVDLDIPQDALLFSITQKPRHGLLIDRGFSKDFSENKQPANPHQKHAPVHSFSMELLKTGMRLTYMHDDSESLADDFTIQLSDGKHKILKTISVEVIPVNDEKPMLSKKAEIAMNMGETRIISSAILSAIDEDSPREKIYYVFERLPQNGQLQLKIGRDWVPLSPGMKCTQEEVDLNLLRYTHTGAMDSQNQDSFTFYLWDGNNRSPALDCQITIKDMEKGDIVILTKPLVVSKGDRGFLTTTTLLAVDGTDKPEELLYVITSPPRYGQIEYVHYPGVPITNFSQMDVVGQTVCYVHKSKVTVSSDRFRFIISNGLRTEHGVFEITLETVDRALPVVTRNKGLRLAQGAVGLLSPDLLQLTDPDTPAENLTFLLVQLPQHGQLYLWGTGLLQHNFTQQDVDSKNVAYRHSGGDSQTDCFTFMATDGTNQGFIVNGRVWEEPVLFTIQVDQLDKTAPRITLLHSPSQVGLLKNGCYGIYITSRVLKASDPDTEDDQIIFKILQGPKHGHLENTTTGEFIHEKFSQKDLNSKTILYIINPSLEVNSDTVEFQIMDPTGNSATPQILELKWSHIEWSQTEYEVCENVGLLPLEIIRRGYSMDSAFVGIKVNQVSAAVGKDFTVIPSKLIQFDPGMSTKMWNIAITYDGLEEDDEVFEVILNSPVNAVLGTKTKAAVKILDSKGGQCHPSYSSNQSKHSTWEKGIWHLLPPGSSSSTTSGSFHLERRPLPSSMQLAVIRGDTLRGFDSTDLSQRKLRTRGNGKTVRPSSVYRNGTDIIYNYHGIVSLKLEDDSFPTHKRKAKVSIISQPQKTIKVAELPQADKVESTTDSHFPRQDQLPSFPKNCTLELKGLFHFEEGIQKLYQCNGIAWKAWSPQTKDVEDKSCPAGWHQHSGYCHILITEQKGTWNAAAQACREQYLGNLVTVFSRQHMRWLWDIGGRKSFWIGLNDQVHAGHWEWIGGEPVAFTNGRRGPSQRSKLGKSCVLVQRQGKWQTKDCRRAKPHNYVCSRKL

Target ORF information:

RefSeq Version	XM_017014326.1
Organism	Homo sapiens(human)
Definition	Homo sapiens FRAS1 related extracellular matrix 1 (FREM1), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017014326.1

ORF Insert Sequence:

ATGAGTAACA ATGTGCTGGA GGTGCCTGAA TTCAATGGCT TGTCCCAAGC GATTGATAAA 
AATCTGCTCA GATTCGATTA TGATAGGATG GCTAGCCTGG AATGTACCGT CAGCCTGGAC 
ACTGCGAGAA CTCGGCTGCC AGCCCATGGC CAGATGGTTC TCGGGGAGCC TCGACCAGAA 
GAACCTCGTG GAGATCAGCC ACACAGTTTT TTCCCAGAGT CCCGTATGAT GTCTTTTAAT 
GCCTTTCTGG AACTGAGAGC AAAACTGAAG TGTCCAGGTG GGAGCTGTAC CCCAGGATTA 
AAGAAAATAG GAAGCCTCAA AGTGAGCTGT GAGGAGTTCC TGCTGATGGG CCTTCGTTAT 
CAGCATCTGG ATCCCCCTTC ACCCAACATT GATTATATCT CCATCCAACT GGACCTCACA 
GATACCAGGA GCAAAATTGT GTACAAGTCA GAGAGTGCGT GGCTGCCTGT CTATATCAGA 
GCTGGAATTC CGAATCAGAT TCCAAAGGCT GCATTCATGG CCGTGTTTAT TCTGGAAGTG 
GATCAGTTCA TCCTGACCTC CTTGACTACA TCAGTTCTGG ACTGTGAAGA AGATGAGACC 
CCTAAACCCT TGCTGGTGTT CAACATTACT AAAGCCCCGC TCCAGGGCTA TGTGACTCAC 
CTGTTGGATC ACACCAGACC AATCTCCTCA TTCACCTGGA AAGATCTCAG TGACATGCAG 
ATCGCCTATC AGCCACCAAA CAGCAGCCAT TCTGAGAGGA GACATGATGA GGTAGAATTG 
GAGGTATACG ACTTCTTCTT TGAAAGGAGT GCACCTATGA CAGTCCACAT CTCCATCAGA 
ACAGCAGATA CAAATGCCCC CCGTGTATCC TGGAATACAG GTCTGAGTCT CCTTGAGGGG 
CAGTCTCGAG CCATCACTTG GGAACAGTTT CAGGTTGTCG ACAATGACGA CATTGGTGCT 
GTCCGGCTAG TCACCGTTGG TGGCCTGCAG CATGGATGGC TGACTTTAAG AGGGGGGAAA 
GGGTTTCTCT TCACCGTGGC TGACCTCCAG GCTGGAGTTG TTCGCTATCA TCATGATGAC 
AGCGACTCCA CCAAAGACTT CGTGGTCTTC CGGATATTTG ATGGCCATCA CAGCATCCGT 
CACAAATTCC CCATCAACGT CTTGCCCAAA GATGATAGTC CCCCGTTCCT CATAACCAAT 
GTTGTGATTG AACTGGAGGA GGGGCAGACC ATCCTGATCC AGGGATCCAT GCTGCGAGCT 
TCAGATGTGG ACGCCAGTGA TGACTACATC TTCTTCAATA TCACAAAGCC TCCACAGGCT 
GGGGAGATCA TGAAGAAGCC AGGGCCAGGA CTGATAGGCT ATCCTGTCCA TGGCTTCCTT 
CAGAGGGATT TGTTTAATGG AATCATTTAT TATCGTCATT TTGGTGGAGA AATCTTTGAA 
GATTCTTTTC AATTTGTCCT GTGGGACAGC CATGAACCTC CAAATCTTTC AGTGCCACAG 
GTGGCAACAA TCCATATAAC TCCAGTGGAT GACCAGCTTC CAAAAGAGGC TCCTGGAGTT 
TCTCGGCATT TGGTTGTCAA GGAAACTGAG GTGGCCTATA TAACTAAGAA ACAGCTACAT 
TTTATAGATT CAGAATCATA TGACAGGGAG CTGGTCTACA CAATAACTAC TCCTCCATTT 
TTCTCCTTCA GCCACAGACA CTTGGATGCT GGGAAATTAT TTATGGTGGA CAGCATACCA 
AAAGTAGTTA AGAATCCTAC GGCCCTGGAG CTGAGGTCAT TCACTCAGCA TGCTGTGAAC 
TATATGAAAG TGGCCTACAT GCCCCCCATG CAAGACATTG GTCCCCATTG CAGAGATGTC 
CAGTTCACAT TTTCTGTCAG TAACCAACAT GGCGGTACTT TGCATGGGAT CTGCTTTAAC 
ATTACAATCC TCCCAGTGGA CAATCAAGTT CCTGAGGCGT TCACCAACCC TCTGAAAGTG 
ACTGAGGGAG GTCAAAGCAT CATCAGCACA GAGCACATTC TAATTTCTGA TGCAGATACC 
AAGCTGGACA ATATTGACCT CTCCCTGCGG GAATTGCCTC TGCACGGAAG GGTGGAGCTG 
AATGGATTTC CTCTAAATTC AGGGGGCACA TTTTCTTGGG GCGATCTCCA TACCTTAAAA 
GTTAGGTATC AACATGATGG AACTGAAGTT CTTCAGGATG ACCTACTCTT GGAGGTCACC 
GATGGCACAA ATTCAGCAGA ATTTGTACTA CATGTTGAGG TATTCCCTGT CAACGATGAG 
CCACCAGTCT TAAAGGCTGA CCTCATGCCT GTCATGAATT GCTCAGAGGG AGGAGAGGTG 
GTCATCACCT CTGAATACAT TTTTGCTACT GATGTGGACA GCGATAACTT GAAGTTGATG 
TTTGTGATTG CTCGCGAACC TCAGCATGGG GTGGTGAGGA GAGCTGGAGT CACAGTGGAT 
CAGTTCTCTC AGAGAGATGT TATCTCAGAG GCCGTGACAT ACAAACACAC AGGTGGCGAG 
ATTGGCCTGA TGCCTTGTTT TGACACCATT ACATTGGTGG TTTCGGATGG AGAGGCTGGC 
CCTTTTGTGA ATGGCTGTTG CTACAATGGA CCCAATCCAT CTGTTCCACT TCATGCGTCC 
TTTCCAGTGT ATGATCTCAA CATCACGGTA TACCCAGTAG ACAACCAGCC ACCTTCCATT 
GCAATAGGTC CCGTGTTTGT TGTAGATGAG GGCTGCTCAA CAGCCCTTAC TGTTAACCAT 
TTGTCCGCCA CTGACCCTGA CACTGCAGCA GATGACTTGG AATTTGTTTT GGTTTCTCCT 
CCTCAGTTTG GCTACCTCGA AAATATACTC CCTTCTGTGG GTTTTGAAAA AAGCAATATT 
GGCATAAGTA TAGATTCATT TCAGTGGAAA GACATGAACG CTTTTCACAT TAACTATGTG 
CAGTCCAGGC ATCTGAGGAT AGAACCAACT GCCGACCAGT TCACGGTGTA CGTCACAGAT 
GGGAAGCATC ACTCCTTGGA GATACCATTT TCTATTATAA TCAACCCCAC AAATGATGAA 
GCTCCTGACT TTGTAGTGCA GAATATTACT GTGTGTGAGG GTCAGATGAA AGAGCTGGAC 
TCTTCCATCA TCAGCGCTGT GGACCTGGAC ATTCCCCAGG ATGCCCTGCT GTTCAGCATC 
ACTCAAAAGC CACGCCATGG CCTCCTCATC GATAGGGGGT TTAGCAAAGA CTTCTCTGAG 
AATAAGCAGC CAGCCAACCC TCACCAGAAA CATGCACCTG TTCACAGCTT TTCCATGGAA 
CTCCTCAAGA CTGGAATGAG GTTGACGTAC ATGCATGATG ACTCAGAGAG CCTTGCTGAT 
GATTTTACAA TCCAATTGTC AGATGGGAAA CATAAGATAC TTAAAACCAT TTCAGTAGAG 
GTCATCCCAG TTAATGATGA AAAACCAATG CTGAGCAAGA AGGCTGAAAT TGCAATGAAT 
ATGGGTGAAA CTCGTATTAT TTCCAGTGCT ATTCTTTCAG CCATAGATGA AGACTCACCC 
AGGGAGAAGA TTTACTATGT ATTTGAAAGG CTTCCCCAAA ATGGGCAACT TCAGCTTAAG 
ATAGGGAGGG ACTGGGTTCC TCTCTCCCCT GGCATGAAAT GCACTCAGGA GGAAGTGGAT 
CTGAACTTGC TGAGATACAC ACACACCGGG GCAATGGATT CCCAGAATCA AGATAGCTTC 
ACCTTCTACC TTTGGGATGG CAACAACAGG TCCCCTGCTC TTGACTGTCA AATCACCATC 
AAGGACATGG AAAAAGGTGA TATTGTCATC CTTACAAAAC CACTCGTGGT GTCTAAAGGT 
GATAGAGGTT TCTTAACAAC CACCACGCTC CTGGCTGTGG ACGGAACAGA CAAGCCTGAG 
GAACTGCTCT ATGTCATCAC CTCCCCTCCG CGATATGGCC AGATCGAATA TGTTCACTAT 
CCTGGAGTTC CCATTACAAA CTTCAGCCAA ATGGATGTAG TGGGGCAGAC AGTTTGCTAT 
GTACACAAGA GCAAGGTGAC TGTCTCCAGT GACAGATTCA GATTCATCAT CAGCAATGGA 
CTGCGGACCG AGCACGGGGT GTTTGAGATC ACACTGGAGA CTGTGGACAG AGCCCTGCCT 
GTGGTAACCA GGAACAAGGG GTTGAGACTG GCCCAAGGGG CCGTGGGCCT GCTTTCCCCT 
GACCTCCTTC AGCTGACCGA CCCTGATACA CCTGCGGAGA ACCTCACCTT CCTCTTGGTT 
CAGCTCCCCC AGCATGGCCA GCTCTACCTG TGGGGGACAG GGCTACTTCA ACACAATTTC 
ACCCAGCAGG ATGTGGACAG CAAGAATGTG GCCTATCGGC ACTCAGGAGG GGACTCCCAG 
ACTGACTGCT TTACTTTCAT GGCCACAGAT GGGACAAACC AAGGCTTTAT TGTGAATGGG 
AGAGTGTGGG AAGAACCTGT TTTATTCACC ATTCAGGTAG ACCAATTGGA CAAAACAGCT 
CCTCGTATCA CACTCTTGCA TTCCCCTTCT CAAGTGGGGC TCCTGAAAAA TGGCTGCTAC 
GGGATTTACA TCACTTCCCG CGTGTTGAAG GCATCAGACC CTGACACTGA GGACGATCAG 
ATCATCTTTA AAATTCTACA AGGCCCAAAA CATGGACATC TGGAGAACAC AACAACAGGT 
GAATTTATCC ATGAGAAATT TAGCCAAAAG GACTTAAACA GTAAGACTAT TCTTTACATC 
ATAAACCCAT CTTTGGAAGT AAATTCAGAT ACCGTGGAAT TTCAAATCAT GGACCCCACA 
GGGAACTCGG CCACTCCTCA AATTTTGGAA CTGAAGTGGT CTCATATTGA ATGGTCACAG 
ACCGAATATG AAGTCTGTGA GAATGTGGGT TTGTTGCCCT TGGAAATTAT CAGAAGGGGA 
TATTCCATGG ACTCGGCCTT TGTGGGTATA AAGGTCAACC AAGTGTCAGC TGCAGTTGGA 
AAAGATTTCA CCGTGATTCC ATCTAAACTG ATTCAGTTTG ACCCAGGAAT GTCAACTAAG 
ATGTGGAATA TAGCAATTAC CTATGACGGA TTAGAGGAAG ATGATGAGGT CTTTGAAGTA 
ATTCTGAACT CCCCTGTGAA TGCAGTTCTT GGCACAAAGA CAAAAGCTGC AGTGAAAATT 
TTGGACTCAA AAGGAGGACA ATGCCATCCT TCATATTCCT CCAACCAAAG CAAGCACAGC 
ACATGGGAGA AGGGCATTTG GCATCTGCTG CCCCCAGGGT CTTCCTCATC CACCACTTCT 
GGTTCCTTTC ATCTGGAAAG AAGACCTCTT CCATCTTCCA TGCAGCTAGC AGTCATCAGG 
GGAGACACCC TGCGGGGCTT TGATTCTACA GATCTTTCTC AAAGGAAGCT TAGGACCCGT 
GGGAATGGCA AAACAGTTCG TCCATCCTCT GTTTATAGAA ATGGAACAGA CATCATCTAT 
AATTATCATG GGATAGTTTC CTTGAAACTG GAGGATGACA GTTTCCCAAC TCACAAAAGG 
AAGGCCAAAG TATCCATCAT TAGTCAGCCA CAAAAGACAA TCAAAGTGGC AGAACTGCCT 
CAAGCAGATA AGGTGGAATC CACAACTGAC TCACACTTCC CCAGACAGGA CCAGTTGCCC 
TCATTTCCAA AGAACTGCAC TCTGGAATTA AAGGGACTCT TCCATTTTGA AGAAGGCATC 
CAGAAGCTGT ATCAGTGCAA TGGGATCGCC TGGAAAGCCT GGAGTCCCCA AACCAAGGAT 
GTGGAAGACA AATCCTGTCC AGCCGGGTGG CACCAGCACT CAGGCTACTG TCACATCTTG 
ATCACAGAGC AGAAAGGCAC CTGGAATGCG GCTGCCCAAG CTTGCAGGGA ACAATACCTG 
GGCAACCTTG TAACTGTATT CTCCAGGCAG CACATGCGGT GGCTCTGGGA CATTGGTGGG 
AGAAAGTCCT TTTGGATAGG TTTGAACGAC CAAGTGCATG CTGGCCACTG GGAGTGGATC 
GGTGGTGAAC CTGTTGCCTT CACCAATGGG AGAAGAGGGC CCTCTCAACG CTCCAAGCTT 
GGAAAGAGCT GTGTTTTGGT TCAAAGACAA GGGAAATGGC AAACAAAAGA CTGTAGGAGA 
GCCAAACCTC ATAATTATGT GTGTTCCAGA AAACTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Human gingiva transcriptome during wound healing.
Journal of clinical periodontology44(4)394-402(2017 Apr)
Wang Y,Tatakis DN

Novel FREM1 mutations in a patient with MOTA syndrome: Clinical findings, mutation update and review of FREM1-related disorders literature.
European journal of medical genetics60(3)190-194(2017 Mar)
Chacon-Camacho OF,Zenker M,Schanze D,Ledesma-Gil J,Zenteno JC

Genome-wide association study of facial morphology reveals novel associations with FREM1 and PARK2.
PloS one12(4)e0176566(2017)
Lee MK,Shaffer JR,Leslie EJ,Orlova E,Carlson JC,Feingold E,Marazita ML,Weinberg SM

Mild recessive mutations in six Fraser syndrome-related genes cause isolated congenital anomalies of the kidney and urinary tract.
Journal of the American Society of Nephrology : JASN25(9)1917-22(2014 Sep)
Kohl S,Hwang DY,Dworschak GC,Hilger AC,Saisawat P,Vivante A,Stajic N,Bogdanovic R,Reutter HM,Kehinde EO,Tasic V,Hildebrandt F

見積依頼

Eメール

japanmarket@genscript.com

電話

03-6811-6572

FAX

03-6811-6573
050-3174-7589(受注専用)

オンラインフォーム

オンライン注文

FREM1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence: